title: A resource of ribosomal RNA-depleted RNA-Seq data from different normal adult and fetal human tissues.
abstract: Gene expression is the most fundamental level at which the genotype leads to the phenotype of the organism. Enabled by ultra-high-throughput next-generation DNA sequencing, RNA-Seq involves shotgun sequencing of fragmented RNA transcripts by next-generation sequencing followed by in silico assembly, and is rapidly becoming the most popular method for gene expression analysis. Poly[A]+ RNA-Seq analyses of normal human adult tissue samples such as Illumina's Human BodyMap 2.0 Project and the RNA-Seq atlas have provided a useful global resource and framework for comparisons with diseased tissues such as cancer. However, these analyses have failed to provide information on poly[A]-RNA, which is abundant in our cells. The most recent advances in RNA-Seq analyses use ribosomal RNA-depletion to provide information on both poly[A]+ and poly[A]-RNA. In this paper, we describe the use of Illumina's HiSeq 2000 to generate high quality rRNA-depleted RNA-Seq datasets from human fetal and adult tissues. The datasets reported here will be useful in understanding the different expression profiles in different tissues.
authors.affiliation: Cancer Science Institute of Singapore, National University of Singapore , Singapore 117599, Singapore.
authors.forename: Jocelyn Y H
authors.initials: JY
authors.lastname: Choy
authors.affiliation: Cancer Science Institute of Singapore, National University of Singapore , Singapore 117599, Singapore.
authors.forename: Priscilla L S
authors.initials: PL
authors.lastname: Boon
authors.affiliation: Cancer Science Institute of Singapore, National University of Singapore , Singapore 117599, Singapore.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Bertin
authors.affiliation: Cancer Science Institute of Singapore, National University of Singapore , Singapore 117599, Singapore ; School of Biological Sciences, Nanyang Technological University , Singapore 637551, Singapore ; Institute of Molecular and Cell Biology, Agency for Science, Technology and Research (ASTAR) , Singapore 138673, Singapore ; Yale-NUS Liberal Arts College , Singapore 138527, Singapore.
authors.forename: Melissa J
authors.initials: MJ
authors.lastname: Fullwood
mesh_terms: D000328:Adult; D005333:Fetus; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D009928:Organ Specificity; D012335:RNA, Ribosomal / Q000235:genetics*; D017423:Sequence Analysis, RNA
chemical_list: D012335:RNA, Ribosomal
keywords: Development; RNA sequencing; Transcriptomics