title: Introducing Cytology-Based Theranostics in Oral Squamous Cell Carcinoma: A Pilot Program.
abstract: We aimed to evaluate the feasibility and reliability of brush cytology in the biomarker expression profiling of oral squamous cell carcinomas within the concept of theranostics, and to correlate this biomarker profile with patient measurable outcomes. Markers representative of prognostic gene expression changes in oral squamous cell carcinoma was selected. These markers were also selected to involve pathways for which commercially available or investigational agents exist for clinical application. A set of 7 markers were analysed by immunocytochemistry on the archival primary tumour material of 99 oral squamous cell carcinoma patients. We confirmed the feasibility of the technique for the expression profiling of oral squamous cell carcinomas. Furthermore, our results affirm the prognostic significance of the epidermal growth factor receptor (EGFR) family and the angiogenic pathway in oral squamous cell carcinoma, confirming their interest for targeted therapy. Brush cytology appears feasible and applicable for the expression profiling of oral squamous cell carcinoma within the concept of theranostics, according to sample availability.
authors.affiliation: Department of Cancer Medicine, Gustave Roussy, Cancer Campus, Grand Paris, 114 rue Edouard Vaillant, 94805, Villejuif, France. anna.patrikidou@gustaveroussy.fr.
authors.forename: Anna
authors.initials: A
authors.lastname: Patrikidou
authors.affiliation: Department of Cytopathology, "Theagenio" Cancer Hospital, Thessaloniki, Greece.
authors.forename: Rosalia Maria
authors.initials: RM
authors.lastname: Valeri
authors.affiliation: Department of Forestry and Management of the Environment and Natural Resources, School of Agricultural Sciences and Forestry, Democritus University, Alexandroupoli, Greece.
authors.forename: Kyriaki
authors.initials: K
authors.lastname: Kitikidou
authors.affiliation: Department of Cytopathology, "Theagenio" Cancer Hospital, Thessaloniki, Greece.
authors.forename: Charikleia
authors.initials: C
authors.lastname: Destouni
authors.affiliation: Department of Oral and Maxillofacial Surgery, "Theagenio" Cancer Hospital, Thessaloniki, Greece.
authors.forename: Konstantinos
authors.initials: K
authors.lastname: Vahtsevanos
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000235:genetics*; D001706:Biopsy; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis* / Q000235:genetics; D003581:Cytodiagnosis / Q000379:methods*; D005260:Female; D005500:Follow-Up Studies; D020869:Gene Expression Profiling; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000175:diagnosis* / Q000235:genetics; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D010865:Pilot Projects; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015996:Survival Rate; D000068936:Theranostic Nanomedicine
chemical_list: D014408:Biomarkers, Tumor; D012333:RNA, Messenger
keywords: Angiogenesis; Biomarker; Cytology; Oral squamous cell carcinoma; Theranostics