title: Thai Silk Fibroin/Gelatin Sponges for the Dual Controlled Release of Curcumin and Docosahexaenoic Acid for Anticancer Treatment.
abstract: In this study, curcumin and/or docosahexaenoic acid (DHA) were encapsulated in Thai silk fibroin/gelatin (SF/G) sponges, prepared at different blending ratios, aimed to be applied as a controlled release system for localized cancer therapy. The SF/G sponges were fabricated by freeze-drying and glutaraldehyde cross-linking techniques. Physicochemical properties of the SF/G sponges were characterized. Then, curcumin and/or DHA were loaded in the sponges by physical adsorption. The encapsulation efficiency and the in vitro release of curcumin and/or DHA from the sponges were evaluated. SF/G sponges could encapsulate curcumin and/or DHA at high encapsulation efficiency. The highly cross-linked and slowly degrading SF/G (50/50) sponge released curcumin and/or DHA at the slowest rate. The in vitro cytotoxicity of the sponges against noncancer cells (L929 mouse fibroblast) and anticancer of curcumin and/or DHA released from the sponges against cervical cancer cells (CaSki) were tested. All sponges were not toxic to L929 mouse fibroblast. The mixed curcumin–DHA at the ratio of 1:4 had the highest inhibiting effect on the growth of CaSki, comparing with the release of curcumin or DHA alone. SF/G sponges could be a potential carrier for dual release of curcumin and DHA for anticancer effect.
authors.forename: Kantarat
authors.initials: K
authors.lastname: Lerdchai
authors.forename: Jutarat
authors.initials: J
authors.lastname: Kitsongsermthon
authors.forename: Juthamas
authors.initials: J
authors.lastname: Ratanavaraporn
authors.forename: Sorada
authors.initials: S
authors.lastname: Kanokpanont
authors.forename: Siriporn
authors.initials: S
authors.lastname: Damrongsakkul
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage* / Q000627:therapeutic use* / Q000633:toxicity; D063566:Biodegradable Plastics / Q000737:chemistry*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003432:Cross-Linking Reagents; D003474:Curcumin / Q000008:administration & dosage* / Q000627:therapeutic use* / Q000633:toxicity; D003692:Delayed-Action Preparations; D004281:Docosahexaenoic Acids / Q000008:administration & dosage* / Q000627:therapeutic use*; D005347:Fibroblasts; D005349:Fibroins / Q000737:chemistry*; D005612:Freeze Drying; D005780:Gelatin / Q000737:chemistry*; D005976:Glutaral; D051379:Mice; D009369:Neoplasms / Q000188:drug therapy*; D016062:Porosity; D047011:Silk / Q000737:chemistry*; D013785:Thailand
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D063566:Biodegradable Plastics; D003432:Cross-Linking Reagents; D003692:Delayed-Action Preparations; D047011:Silk; D004281:Docosahexaenoic Acids; D005780:Gelatin; D005349:Fibroins; D003474:Curcumin; D005976:Glutaral