title: LRP1B, BRD2 and CACNA1D: new candidate genes in fetal metabolic programming of newborns exposed to maternal hyperglycemia.
abstract: OBJECTIVE
  To assess the associations between gestational diabetes mellitus (GDM) and DNA methylation levels at genes related to energy metabolism.
  
  
  METHODS
  Ten loci were selected from our recent epigenome-wide association study on GDM. DNA methylation levels were quantified by bisulfite pyrosequencing in 80 placenta and cord blood samples (20 exposed to GDM) from an independent birth cohort (Gen3G).
  
  
  RESULTS
  We did not replicate association between DNA methylation and GDM. However, in normoglycemic women, glucose levels were associated with DNA methylation changes at LRP1B and BRD2 and at CACNA1D and LRP1B gene loci in placenta and cord blood, respectively.
  
  
  CONCLUSIONS
  These results suggest that maternal glucose levels, within the normal range, are associated with DNA methylation changes at genes related to energy metabolism and previously associated with GDM. Maternal glycemia might thus be involved in fetal metabolic programming.
authors.affiliation: Department of Biochemistry, Université de Sherbrooke, Sherbrooke, QC, Canada.|ECOGENE-21 & Clinical Research Center & Lipid Clinic, Chicoutimi Hospital, Saguenay, QC, Canada.
authors.forename: Andrée-Anne
authors.initials: AA
authors.lastname: Houde
authors.affiliation: Department of Biochemistry, Université de Sherbrooke, Sherbrooke, QC, Canada.|ECOGENE-21 & Clinical Research Center & Lipid Clinic, Chicoutimi Hospital, Saguenay, QC, Canada.
authors.forename: Stephanie-May
authors.initials: SM
authors.lastname: Ruchat
authors.affiliation: Department of Medicine, Division of Endocrinology, Université de Sherbrooke, Sherbrooke, QC, Canada.
authors.forename: Catherine
authors.initials: C
authors.lastname: Allard
authors.affiliation: Department of Medicine, Division of Endocrinology, Université de Sherbrooke, Sherbrooke, QC, Canada.
authors.forename: Jean-Patrice
authors.initials: JP
authors.lastname: Baillargeon
authors.affiliation: ECOGENE-21 & Clinical Research Center & Lipid Clinic, Chicoutimi Hospital, Saguenay, QC, Canada.|Department of Pediatrics, Chicoutimi Hospital, Saguenay, QC, Canada.|Department of Health Sciences, Université du Québec à Chicoutimi, Saguenay, QC, Canada.
authors.forename: Julie
authors.initials: J
authors.lastname: St-Pierre
authors.affiliation: ECOGENE-21 & Clinical Research Center & Lipid Clinic, Chicoutimi Hospital, Saguenay, QC, Canada.|Department of Medicine, Division of Endocrinology, Université de Sherbrooke, Sherbrooke, QC, Canada.
authors.forename: Patrice
authors.initials: P
authors.lastname: Perron
authors.affiliation: ECOGENE-21 & Clinical Research Center & Lipid Clinic, Chicoutimi Hospital, Saguenay, QC, Canada.|Department of Medicine, Université de Montréal, Montréal, QC, Canada.
authors.forename: Daniel
authors.initials: D
authors.lastname: Gaudet
authors.affiliation: ECOGENE-21 & Clinical Research Center & Lipid Clinic, Chicoutimi Hospital, Saguenay, QC, Canada.|Department of Medicine, Université de Montréal, Montréal, QC, Canada.
authors.forename: Diane
authors.initials: D
authors.lastname: Brisson
authors.affiliation: Department of Medicine, Division of Endocrinology, Université de Sherbrooke, Sherbrooke, QC, Canada.|Department of Population Medicine, Harvard Pilgrim Health Care Institute, Boston, MA, USA.|General Medicine Division, Massachusetts General Hospital, Boston, MA, USA.
authors.forename: Marie-France
authors.initials: MF
authors.lastname: Hivert
authors.affiliation: Department of Biochemistry, Université de Sherbrooke, Sherbrooke, QC, Canada.|ECOGENE-21 & Clinical Research Center & Lipid Clinic, Chicoutimi Hospital, Saguenay, QC, Canada.
authors.forename: Luigi
authors.initials: L
authors.lastname: Bouchard
mesh_terms: D000328:Adult; D001724:Birth Weight; D001786:Blood Glucose / Q000378:metabolism; D020746:Calcium Channels, L-Type / Q000235:genetics* / Q000378:metabolism; D015331:Cohort Studies; D019175:DNA Methylation; D016640:Diabetes, Gestational / Q000097:blood / Q000235:genetics* / Q000503:physiopathology; D004734:Energy Metabolism / Q000235:genetics; D044127:Epigenesis, Genetic; D005260:Female; D005312:Fetal Blood / Q000378:metabolism; D047109:Fetal Development / Q000235:genetics; D005333:Fetus; D006801:Humans; D006943:Hyperglycemia / Q000097:blood / Q000235:genetics* / Q000503:physiopathology; D007231:Infant, Newborn; D008297:Male; D010920:Placenta / Q000378:metabolism; D011247:Pregnancy; D017346:Protein Serine-Threonine Kinases / Q000235:genetics* / Q000378:metabolism; D011973:Receptors, LDL / Q000235:genetics* / Q000378:metabolism; D014157:Transcription Factors; D000092468:Bromodomain Containing Proteins
chemical_list: D001786:Blood Glucose; D020746:Calcium Channels, L-Type; D017346:Protein Serine-Threonine Kinases; D011973:Receptors, LDL; D014157:Transcription Factors; C098415:BRD2 protein, human; D000092468:Bromodomain Containing Proteins; C472073:CACNA1D protein, human; C434476:LRP1B protein, human
keywords: DNA methylation; cord blood; epigenetics; gestational diabetes; placenta