title: Calibration transfer from powder mixtures to intact tablets: A new use in pharmaceutical analysis for a known tool.
abstract: Calibration transfer is commonly used for spectra obtained in different spectrometers or other conditions. This paper proposed the use of calibration transfer between spectra recorded for the same samples in different physical forms. A new method was developed for the direct determination of nevirapine in solid pharmaceutical formulations based on diffuse reflectance near infrared spectroscopy (NIRS) and partial least squares (PLS). This method was developed with 50 powder mixtures and then, successfully extended to the quantification in intact tablets by using calibration transfer with double window piecewise direct standardization (DWPDS). This chemometric strategy provided good results with a small number of tablet transfer samples, only seven, prepared out of the narrow range of active principle ingredients (API) content around the nominal value of the formulation (100%). The method was fully validated in the working range of 83.0-113.9% of nevirapine and the use of DWPDS allowed to significantly decreasing the root mean square error of prediction (RMSEP) from 4.8% (tablets predicted by a model built with only powder samples) to 2.6%. The range of relative errors decreased from -5.1/8.7% to -4.6/3.3%. Considering that the amount of raw materials demanded for preparing tablets is up to ten times higher than for powder mixtures, this type of application is of particular interest in pharmaceutical analysis. In the context of process analytical technology (PAT), the use of the same multivariate model in different steps of the production is very advantageous, saving time and labor.
authors.affiliation: Departamento de Química, ICEx, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, MG, Brazil.
authors.forename: Leandro S A
authors.initials: LS
authors.lastname: Pereira
authors.affiliation: Fundação Ezequiel Dias (FUNED), 30510-010 Belo Horizonte, MG, Brazil.
authors.forename: Maíra F
authors.initials: MF
authors.lastname: Carneiro
authors.affiliation: Departamento de Química, ICEx, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, MG, Brazil.
authors.forename: Bruno G
authors.initials: BG
authors.lastname: Botelho
authors.affiliation: Departamento de Química, ICEx, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, MG, Brazil; Instituto Nacional de Ciência e Tecnologia em Bioanalítica, 13083-970 Campinas, SP, Brazil. Electronic address: marcsen@ufmg.br.
authors.forename: Marcelo M
authors.initials: MM
authors.lastname: Sena
mesh_terms: D002138:Calibration; D015999:Multivariate Analysis; D019829:Nevirapine / Q000032:analysis* / Q000737:chemistry; D011208:Powders; D019265:Spectroscopy, Near-Infrared / Q000379:methods*; D013607:Tablets
chemical_list: D011208:Powders; D013607:Tablets; D019829:Nevirapine
keywords: Calibration transfer; Double window piecewise direct standardization; Intact tablets; Near-infrared spectroscopy; Nevirapine; Pharmaceutical quality control