title: Antidermatophytic activity of hydroalcoholic extracts from Rosmarinus officinalis and Tetradenia riparia.
abstract: Rosmarinus officinalis and Tetradenia riparia are used in folk medicine for the treatment of disease, including infectious diseases and skin disorders. The purpose of this study was to investigate the antifungal activity of hydroalcoholic extracts from R. officinalis and T. riparia against strains of Trichophyton rubrum, T. mentagrophytes and Microsporum gypseum. Hydroalcoholic extracts prepared with dried leaves from R. officinalis, Psidium guajava and T. riparia were assayed against dermatophyte species by the microdilution technique and by microscopy. R. officinalis and T. riparia were the most active against dermatophytes, as determined from the minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC), and were investigated further. Fluorescence microscopy and scanning electron microscopy were used to investigate inhibition of hyphal growth by the two extracts, and showed a strong inhibition and an irregular growth pattern. Both extracts showed good action against dermatophytes, inhibiting fungal growth and causing alterations in their hyphae. Therefore, R. officinalis and T. riparia are potential sources of new compounds for the development of antifungal drugs.
authors.affiliation: Departament of Pharmacy, Universidade Estadual de Maringá, Maringá, PR, Brazil.
authors.forename: E H
authors.initials: EH
authors.lastname: Endo
authors.affiliation: Departament of Pharmacy, Universidade Estadual de Maringá, Maringá, PR, Brazil.
authors.forename: G M
authors.initials: GM
authors.lastname: Costa
authors.affiliation: Department of Health Sciences, Universidade Estadual de Maringá, Maringá, PR, Brazil.
authors.forename: T U
authors.initials: TU
authors.lastname: Nakamura
authors.affiliation: Department of Health Sciences, Universidade Estadual de Maringá, Maringá, PR, Brazil.
authors.forename: C V
authors.initials: CV
authors.lastname: Nakamura
authors.affiliation: Department of Health Sciences, Universidade Estadual de Maringá, Maringá, PR, Brazil. Electronic address: bpdfilho@uem.br.
authors.forename: B P
authors.initials: BP
authors.lastname: Dias Filho
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D003883:Arthrodermataceae / Q000187:drug effects* / Q000254:growth & development / Q000648:ultrastructure; D000431:Ethanol / Q000737:chemistry; D019686:Lamiaceae / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D027542:Rosmarinus / Q000737:chemistry*; D014867:Water / Q000737:chemistry
chemical_list: D000935:Antifungal Agents; D010936:Plant Extracts; D014867:Water; D000431:Ethanol
keywords: Anti-dermatophyte; Antifongique; Dermatophytes; Rosmarinus officinalis; Tetradenia riparia