title: Protective effect of butyrate against ethanol-induced gastric ulcers in mice by promoting the anti-inflammatory, anti-oxidant and mucosal defense mechanisms.
abstract: Gastric ulcers (GUs) are a common type of peptic ulcer. Alcohol overdose is one of the main causes of GU, which is difficult to prevent. Although the protective effect of butyrate on inflammation-related diseases is well understood, its effect on GUs has not been reported. We investigated the protective effects of butyrate against ethanol-induced lesions to the gastric mucosa in mice and the underlying mechanisms. BALB/c mice were orally pretreated with butyrate for 30min prior to the establishment of the GU model by challenge with absolute ethanol. Ethanol administration produced apparent mucosal injuries with morphological and histological damage, whereas butyrate pretreatment reduced the gastric mucosal injuries in a dose-dependent manner. Butyrate pretreatment also significantly ameliorated contents of malondialdehyde (MDA) and carbonyl proteins, and decreased levels of IL-1β, TNF-α and IL-6. The Western blot results consistently demonstrated that butyrate pretreatment attenuated the phosphorylation of NF-κB p65, p38 MAPK and ERKs in the gastric tissues. Additionally, gastric wall mucus (GWM), a parameter reflecting mucosal defense, was clearly increased by butyrate pretreatment. Butyrate pretreatment protects the gastric mucosa against ethanol-induced lesions by strengthening the mucosal defense and anti-oxidant and anti-inflammatory activities. As a necessary substance for the body, butyrate may be applied to the prevention and treatment of GUs.
authors.affiliation: School of Environmental Science and Public Health, Wenzhou Medical University, Wenzhou 325035, Zhejiang Province, China.
authors.forename: Jiaming
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Pathophysiology, Wenzhou Medical University, Wenzhou 325035, Zhejiang Province, China.
authors.forename: Fangyan
authors.initials: F
authors.lastname: Wang
authors.affiliation: Key Laboratory of Transcriptomics and Proteomics of Ministry of Education of China and Key Laboratory of Proteomics of Guangdong Province, Southern Medical University, Guangzhou 510515, China.
authors.forename: Haihua
authors.initials: H
authors.lastname: Luo
authors.affiliation: Key Laboratory of Transcriptomics and Proteomics of Ministry of Education of China and Key Laboratory of Proteomics of Guangdong Province, Southern Medical University, Guangzhou 510515, China.
authors.forename: Aihua
authors.initials: A
authors.lastname: Liu
authors.affiliation: Key Laboratory of Transcriptomics and Proteomics of Ministry of Education of China and Key Laboratory of Proteomics of Guangdong Province, Southern Medical University, Guangzhou 510515, China.
authors.forename: Kangxin
authors.initials: K
authors.lastname: Li
authors.affiliation: Key Laboratory of Transcriptomics and Proteomics of Ministry of Education of China and Key Laboratory of Proteomics of Guangdong Province, Southern Medical University, Guangzhou 510515, China.
authors.forename: Cui
authors.initials: C
authors.lastname: Li
authors.affiliation: Key Laboratory of Transcriptomics and Proteomics of Ministry of Education of China and Key Laboratory of Proteomics of Guangdong Province, Southern Medical University, Guangzhou 510515, China. Electronic address: jiang48231@163.com.
authors.forename: Yong
authors.initials: Y
authors.lastname: Jiang
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage*; D000975:Antioxidants / Q000008:administration & dosage*; D002087:Butyrates / Q000008:administration & dosage*; D016207:Cytokines / Q000378:metabolism; D000431:Ethanol / Q000008:administration & dosage / Q000009:adverse effects; D006801:Humans; D018928:Immunity, Mucosal / Q000187:drug effects; D018836:Inflammation Mediators / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D016328:NF-kappa B / Q000378:metabolism; D013276:Stomach Ulcer / Q000139:chemically induced / Q000517:prevention & control*; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D002087:Butyrates; D016207:Cytokines; D018836:Inflammation Mediators; D016328:NF-kappa B; D000431:Ethanol; D008315:Malondialdehyde; D048051:p38 Mitogen-Activated Protein Kinases
keywords: Butyrate; Cytokine; Gastric ulcer; Inflammation; Stress