title: α-Viniferin and resveratrol induced alteration in the activities of some energy metabolism related enzymes in the cestode parasite Raillietina echinobothrida.
abstract: α-Viniferin (AVF) and its monomer resveratrol (RESV) are natural phytostilbenes produced by several plants in response to injury or under the influence of pathogens such as bacteria or fungi. Our earlier studies have revealed that both the compounds exert anthelmintic activity through alterations of cestode tegument and its associated enzymes. The present study investigates the effects of these phytochemicals on some energy metabolism related enzymes in the fowl tapeworm, Raillietina echinobothrida. The phytostilbenes AVF, RESV and the reference drug praziquantel (PZQ) were tested against some selected enzymes i.e., phosphoenolpyruvate carboxykinase (PEPCK), lactate dehydrogenase (LDH) and malate dehydrogenase (MDH) of R. echinobothrida. Exposure of the tapeworm to AVF, RESV and PZQ causes reduction in activity of PEPCK to the extent of 40.57/41.96, 24.58/23.75 and 41.11/13.47%, respectively, and LDH up to 48.95/16.25, 38.31/38.42 and 45.67/41.87%, respectively, at the time of paralysis. Whereas activity of MDH decreased by 34.22/37.7, 39.1/35.24 and 28.83/19.26%, respectively. Decrease in activities of LDH and MDH was also visible through histochemical observations. The results suggest that both the phytochemicals interfere with the energy transducing pathways by inhibiting the studied energy metabolism related enzymes of the parasite.
authors.affiliation: Parasitology Laboratory, Department of Zoology, North-Eastern Hill University, Shillong, 793022 Meghalaya, India. Electronic address: bishnuroy9@gmail.com.
authors.forename: Bishnupada
authors.initials: B
authors.lastname: Roy
authors.affiliation: Parasitology Laboratory, Department of Zoology, North-Eastern Hill University, Shillong, 793022 Meghalaya, India.
authors.forename: Bikash Ranjan
authors.initials: BR
authors.lastname: Giri
mesh_terms: D000818:Animals; D000871:Anthelmintics / Q000494:pharmacology; D001572:Benzofurans / Q000494:pharmacology*; D002589:Cestoda / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D004734:Energy Metabolism / Q000187:drug effects*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D007770:L-Lactate Dehydrogenase / Q000187:drug effects* / Q000378:metabolism; D008291:Malate Dehydrogenase / Q000187:drug effects* / Q000378:metabolism; D019764:Phosphoenolpyruvate Carboxykinase (ATP) / Q000187:drug effects* / Q000378:metabolism; D011223:Praziquantel / Q000494:pharmacology; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*
chemical_list: D000871:Anthelmintics; D001572:Benzofurans; D004791:Enzyme Inhibitors; D013267:Stilbenes; C064176:alpha-viniferin; D011223:Praziquantel; D007770:L-Lactate Dehydrogenase; D008291:Malate Dehydrogenase; D019764:Phosphoenolpyruvate Carboxykinase (ATP); D000077185:Resveratrol
keywords: Enzymes activities; Raillietina echinobothrida; Resveratrol; α-Viniferin