title: Development of an immunoaffinity chromatography and HPLC-UV method for determination of 16 sulfonamides in feed.
abstract: A novel and simple method for detecting 16 sulfonamides (SAs) in animal feed using high performance liquid chromatography equipped with a photo-diode array detector (HPLC/PDA) and immunoaffinity chromatography was developed. The chromatographic peaks of the 16 SAs were successfully identified by comparing their retention times and UV spectra with reference standards. Method validation was performed with linearity, sensitivity, selectivity, accuracy and precision. The limits of detection (LODs) for the instrument used to study sulfonamides ranged from 14.1 to 45.0 μg/kg, and the limits of quantification (LOQs) ranged from 46.9 to 150.0 μg/kg. Average recoveries of the 16 SAs ranged from 78.2% to 105.2%. Method replication resulted in intraday and interday peak area variation of <5.5%. The developed method was specific and reliable and is suited for the routine analysis of SAs in animal feed.
authors.affiliation: National Agricultural Products Quality Management Service, Kimchun 740-871, Republic of Korea; School of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University, Daegu 702-701, Republic of Korea.
authors.forename: Ho Jin
authors.initials: HJ
authors.lastname: Kim
authors.affiliation: National Agricultural Products Quality Management Service, Kimchun 740-871, Republic of Korea.
authors.forename: Min Hee
authors.initials: MH
authors.lastname: Jeong
authors.affiliation: National Agricultural Products Quality Management Service, Kimchun 740-871, Republic of Korea.
authors.forename: Hye Jin
authors.initials: HJ
authors.lastname: Park
authors.affiliation: School of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University, Daegu 702-701, Republic of Korea.
authors.forename: Won Chan
authors.initials: WC
authors.lastname: Kim
authors.affiliation: School of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University, Daegu 702-701, Republic of Korea. Electronic address: jekim@knu.ac.kr.
authors.forename: Jang Eok
authors.initials: JE
authors.lastname: Kim
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D002846:Chromatography, Affinity / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D057230:Limit of Detection; D015203:Reproducibility of Results; D013449:Sulfonamides / Q000032:analysis*
chemical_list: D013449:Sulfonamides
keywords: Animal feed; Immunoaffinity chromatography; Sulfonamide