title: Protective Effect of Thalidomide on Liver Injury in Rats with Acute Pancreatitis via Inhibition of Oxidative Stress.
abstract: This study was designed to investigate the preventive effect of thalidomide on acute pancreatitis-associated liver injury in the rat and analyze its relationship with oxidative stress. The acute pancreatitis of rats was induced by the retrograde injection of 5% sodium taurocholate into the biliopancreatic duct. Thalidomide (100 mg/kg) was given daily via the intragastric route for 8 days before this injection. The levels of oxidative stress parameters including superoxide dismutase (SOD), glutathione peroxidase (GSHpx), and malondialdehyde (MDA) in the liver were detected by biochemical assay. Nuclear factor-κB p65 (NF-κBp65), tumor necrosis factor α (TNF-α), and intercellular adhesion molecule-1 (ICAM-1) protein and mRNA levels in the liver were detected using western blots and reverse transcriptase polymerase chain reaction, respectively. Compared with the untreated model group, liver histopathology, SOD, GSHpx, MDA levels, NF-κBp65, TNF-α, ICAM-1 protein, and mRNA levels in the liver of rats given thalidomide were improved significantly. Results demonstrate that thalidomide may exert its effects on oxidative stress to attenuate the progression of acute pancreatitis-associated liver injury in rats.
authors.affiliation: Department of Gastroenterology, Jining First People's Hospital, Jining, China jnlvpeng@163.com.
authors.forename: Peng
authors.initials: P
authors.lastname: Lv
authors.affiliation: Department of Gastroenterology, Jining First People's Hospital, Jining, China.
authors.forename: Li-Juan
authors.initials: LJ
authors.lastname: Fan
authors.affiliation: Department of Gastroenterology, Jining First People's Hospital, Jining, China.
authors.forename: Hong-Yun
authors.initials: HY
authors.lastname: Li
authors.affiliation: Department of Gastroenterology, Jining First People's Hospital, Jining, China.
authors.forename: Qing-Shun
authors.initials: QS
authors.lastname: Meng
authors.affiliation: Department of Gastroenterology, Jining First People's Hospital, Jining, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics / Q000378:metabolism; D008107:Liver Diseases / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D010195:Pancreatitis / Q000150:complications* / Q000503:physiopathology; D020011:Protective Agents / Q000494:pharmacology; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism; D013792:Thalidomide / Q000494:pharmacology*; D051996:Transcription Factor RelA / Q000235:genetics / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism
chemical_list: D015415:Biomarkers; D020011:Protective Agents; C498145:Rela protein, rat; D051996:Transcription Factor RelA; D014409:Tumor Necrosis Factor-alpha; D018799:Intercellular Adhesion Molecule-1; D008315:Malondialdehyde; D013792:Thalidomide; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase
keywords: acute pancreatitis; liver injury; nuclear factor-κB; oxidative stress; thalidomide