title: [The cardiac injury effect of microwave radiation on rabbit and its mechanism].
abstract: OBJECTIVE
  To investigate the cardiac injury effect of different intensities microwave radiation on rabbits and its possible mechanism.
  
  
  METHODS
  Rabbits were radiated by intensity of 50, 100, 150 and 200 mW/cm2 2450 MHz microwave for 20 min. 6 h after microwave radiation, the heart tissue was taken. ATP and mitochondria complex IV and V were measured in myocardial cells. The changes of myocardial tissue were observed by light microscopic. The expression of Caspase-3 and HSP 70 were detected by western blotting.
  
  
  RESULTS
  The activity of ATP and mitochondria complex IV and V decreased significantly compared with normal control in cardiac tissue. 100, 150 and 200 mW/cm2 microwave radiation group vs. control group (P <0. 05). The HE staining result showed that myocardial cell appears edema, muscle fiber malalignment, cells appeared obvious injury. Results of western blotting showed that the expression of Caspase-3 and HSP 70 protein increased significantly in different dosage radiation group (P <0. 05).
  
  
  CONCLUSIONS
  Microwave radiation has injury effect on rabbit heart. The possible mechanism may be related with inducing cell apoptosis by changing of stress level in myocardial cell.
authors.forename: Wenhe
authors.initials: W
authors.lastname: Zhu
authors.forename: Nan
authors.initials: N
authors.lastname: Shen
authors.forename: Xiuhong
authors.initials: X
authors.lastname: Zhong
authors.forename: Jianchen
authors.initials: J
authors.lastname: Hou
authors.forename: Shijie
authors.initials: S
authors.lastname: Lü
authors.forename: Jianhui
authors.initials: J
authors.lastname: Cai
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000528:radiation effects*; D015153:Blotting, Western; D053148:Caspase 3 / Q000528:radiation effects*; D018840:HSP70 Heat-Shock Proteins / Q000528:radiation effects*; D006335:Heart Injuries; D008872:Microwaves / Q000009:adverse effects*; D011506:Proteins; D011817:Rabbits
chemical_list: D018840:HSP70 Heat-Shock Proteins; D011506:Proteins; D053148:Caspase 3