title: Nanoporous microscale microbial incubators.
abstract: Reconstruction of phylogenetic trees based on 16S rRNA gene sequencing reveals abundant microbial diversity that has not been cultured in the laboratory. Many attribute this so-called 'great plate count anomaly' to traditional microbial cultivation techniques, which largely facilitate the growth of a single species. Yet, it is widely recognized that bacteria in nature exist in complex communities. One technique to increase the pool of cultivated bacterial species is to co-culture multiple species in a simulated natural environment. Here, we present nanoporous microscale microbial incubators (NMMI) that enable high-throughput screening and real-time observation of multi-species co-culture. The key innovation in NMMI is that they facilitate inter-species communication while maintaining physical isolation between species, which is ideal for genomic analysis. Co-culture of a quorum sensing pair demonstrates that the NMMI can be used to culture multiple species in chemical communication while monitoring the growth dynamics of individual species.
authors.affiliation: Department of Mechanical Engineering, Massachusetts Institute of Technology, USA. crb@mit.edu.
authors.forename: Zhifei
authors.initials: Z
authors.lastname: Ge
authors.affiliation: Department of Organismic and Evolutionary Biology, Harvard University, USA.
authors.forename: Peter R
authors.initials: PR
authors.lastname: Girguis
authors.affiliation: Department of Mechanical Engineering, Massachusetts Institute of Technology, USA. crb@mit.edu.
authors.forename: Cullen R
authors.initials: CR
authors.lastname: Buie
mesh_terms: D019149:Bioreactors; D004926:Escherichia coli / Q000254:growth & development*; D058608:Nanopores; D016062:Porosity