title: Identification of General and Heart-Specific miRNAs in Sheep (Ovis aries).
abstract: MicroRNAs (miRNAs or miRs) are small regulatory RNAs crucial for modulation of signaling pathways in multiple organs. While the link between miRNAs and heart disease has grown more readily apparent over the past three years, these data are primarily limited to small animal models or cell-based systems. Here, we performed a high-throughput RNA sequencing (RNAseq) analysis of left ventricle and other tissue from a pre-clinical ovine model. We identified 172 novel miRNA precursors encoding a total of 264 mature miRNAs. Notably, 84 precursors were detected in both the left ventricle and other tissues. However, 10 precursors, encoding 11 mature sequences, were specific to the left ventricle. Moreover, the total 168 novel miRNA precursors included 22 non-conserved ovine-specific sequences. Our data identify and characterize novel miRNAs in the left ventricle of sheep, providing fundamental new information for our understanding of protein regulation in heart and other tissues.
authors.affiliation: Department of Molecular Virology, Immunology and Medical Genetics, Comprehensive Cancer Center, The Ohio State University, Columbus, OH, United States of America.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Laganà
authors.affiliation: Department of Molecular Virology, Immunology and Medical Genetics, Comprehensive Cancer Center, The Ohio State University, Columbus, OH, United States of America.|Department of Clinical and Molecular Biomedicine, University of Catania, Catania, Italy.
authors.forename: Dario
authors.initials: D
authors.lastname: Veneziano
authors.affiliation: Department of Surgery, The Ohio State University, Columbus, OH, United States of America.
authors.forename: Tyler
authors.initials: T
authors.lastname: Spata
authors.affiliation: Department of Surgery, The Ohio State University, Columbus, OH, United States of America.
authors.forename: Richard
authors.initials: R
authors.lastname: Tang
authors.affiliation: Department of Surgery, The Ohio State University, Columbus, OH, United States of America.
authors.forename: Hua
authors.initials: H
authors.lastname: Zhu
authors.affiliation: The Davis Heart and Lung Research Institute, Departments of Physiology & Cell Biology and Internal Medicine, The Ohio State University Medical Center, Columbus, OH, United States of America.
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Mohler
authors.affiliation: Department of Surgery, The Ohio State University, Columbus, OH, United States of America.
authors.forename: Ahmet
authors.initials: A
authors.lastname: Kilic
mesh_terms: D000818:Animals; D001483:Base Sequence; D016000:Cluster Analysis; D019295:Computational Biology; D015723:Gene Library; D023281:Genomics; D059014:High-Throughput Nucleotide Sequencing; D035683:MicroRNAs / Q000737:chemistry / Q000235:genetics*; D058977:Molecular Sequence Annotation; D008969:Molecular Sequence Data; D009206:Myocardium / Q000378:metabolism*; D009690:Nucleic Acid Conformation; D009928:Organ Specificity / Q000235:genetics; D015203:Reproducibility of Results; D012756:Sheep / Q000235:genetics*
chemical_list: D035683:MicroRNAs