title: The Role of Circulating Tight Junction Proteins in Evaluating Blood Brain Barrier Disruption following Intracranial Hemorrhage.
abstract: Brain injury after intracranial hemorrhage (ICH) results in significant morbidity and mortality. Blood brain barrier (BBB) disruption is a hallmark of ICH-induced brain injury; however, data mirroring BBB disruption in human ICH are scarce. The aim of this study was to assess the significance of circulating biomarkers in evaluating BBB disruption after ICH. Twenty-two patients with ICH were recruited in this study. Concentrations of the tight junction proteins (TJs) Claudin-5 (CLDN5), Occludin (OCLN), and zonula occludens 1 (ZO-1) and vascular endothelial growth factor (VEGF) and matrix metalloproteinase-9 (MMP-9) were measured by using enzyme-linked immunosorbent assay in serum and cerebrospinal fluid (CSF) samples obtained from patients with ICH. The white blood cell (WBC) count in blood and CSF, albumin (ALB) levels in the CSF (ALBCSF), and the BBB ratio were significantly higher in the ICH than in controls (p < 0.05). Significantly higher levels of CLDN5, OCLN, ZO-1, MMP-9, and VEGF in CSF were observed in the ICH group; these biomarkers were also positively associated with BBB ratio (p < 0.05). Our data revealed that circulating TJs could be considered the potential biomarkers reflecting the integrity of the BBB in ICH.
authors.affiliation: Department of Cell Biology and Genetics, Shantou University Medical College, Guangdong 515041, China.
authors.forename: Xiaoyang
authors.initials: X
authors.lastname: Jiao
authors.affiliation: Shantou University Medical College, Guangdong 515041, China.
authors.forename: Ping
authors.initials: P
authors.lastname: He
authors.affiliation: Department of Cell Biology and Genetics, Shantou University Medical College, Guangdong 515041, China.
authors.forename: Yazhen
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Cell Biology and Genetics, Shantou University Medical College, Guangdong 515041, China.
authors.forename: Zhicheng
authors.initials: Z
authors.lastname: Fan
authors.affiliation: Department of Cell Biology and Genetics, Shantou University Medical College, Guangdong 515041, China.
authors.forename: Mengya
authors.initials: M
authors.lastname: Si
authors.affiliation: Department of Cell Biology and Genetics, Shantou University Medical College, Guangdong 515041, China.
authors.forename: Qingdong
authors.initials: Q
authors.lastname: Xie
authors.affiliation: Department of Cell Biology and Genetics, Shantou University Medical College, Guangdong 515041, China.
authors.forename: Xiaolan
authors.initials: X
authors.lastname: Chang
authors.affiliation: Department of Cell Biology and Genetics, Shantou University Medical College, Guangdong 515041, China.
authors.forename: Dongyang
authors.initials: D
authors.lastname: Huang
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood / Q000134:cerebrospinal fluid; D001812:Blood-Brain Barrier / Q000473:pathology*; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D020300:Intracranial Hemorrhages / Q000097:blood / Q000134:cerebrospinal fluid* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D062725:Tight Junction Proteins / Q000097:blood / Q000134:cerebrospinal fluid*
chemical_list: D015415:Biomarkers; D062725:Tight Junction Proteins