title: Evaluation of the Proliferative Effects Induced by Low-Level Laser Therapy in Bone Marrow Stem Cell Culture.
abstract: OBJECTIVE
  The objective of this study was to evaluate the effect of laser irradiation on dog bone marrow stem cells.
  
  
  BACKGROUND
  Low doses of low-level red laser positively affect the viability of mesenchymal stem cells, and also increase proliferation.
  
  
  METHODS
  Low-level laser (wavelength, 660 nm; power output, 50 mW), was applied to dog bone marrow stem cell cultures (DBMSC). The energy densities delivered varied from 1 to 12J/cm(2). The effect of the laser irradiation was evaluated on cell proliferation measured with the MTT colorimetric test, cell cycle phase, and on lipidic peroxidation (free radical production).
  
  
  RESULTS
  The results indicate that laser irradiation to DBMSC did not change the morphology of the cells, but significantly increased their viability and the number of cells at the G2/M phase with 6, 10, and 12 J/cm(2). On the other hand, malonaldehyde production was significantly enhanced with 8 J/cm(2).
  
  
  CONCLUSIONS
  The parameters used to irradiate DBMSC increased significantly proliferation without producing high levels of reactive oxygen species (ROS).
authors.affiliation: 1 Postgraduate Department, Cruzeiro do Sul University São Paulo , SP, Brasil .|2 UMR-7365, Faculté de Médecine, CNRS-Université de Lorraine , Vandoeuvre-lés-Nancy, France .
authors.forename: Marcos Fernando Xisto Braga
authors.initials: MF
authors.lastname: Cavalcanti
authors.affiliation: 3 Biochemistry and Biophysics Laboratory of Institute Butantan São Paulo , SP, Brasil .
authors.forename: Durvanei A
authors.initials: DA
authors.lastname: Maria
authors.affiliation: 2 UMR-7365, Faculté de Médecine, CNRS-Université de Lorraine , Vandoeuvre-lés-Nancy, France .
authors.forename: Natalia
authors.initials: N
authors.lastname: de Isla
authors.affiliation: 4 Center for Research and Innovation in Laser , São Paulo, SP, Brasil .
authors.forename: Ernesto Cesar Pinto
authors.initials: EC
authors.lastname: Leal-Junior
authors.affiliation: 5 Institute for Physiotherapy, Bergen University College , Bergen Norway.
authors.forename: Jon
authors.initials: J
authors.lastname: Joensen
authors.affiliation: 6 Phisiotherapy Research Group, Department of Global and Public Health, University of Bergen , Bergen Norway.
authors.forename: Jan Magnus
authors.initials: JM
authors.lastname: Bjordal
authors.affiliation: 7 Biomedicine Engineering, Mogi das Cruzes University , Mogi das Cruzes, SP, Brasil .
authors.forename: Rodrigo A M B
authors.initials: RA
authors.lastname: Lopes-Martins
authors.affiliation: 8 Department of Medical, Oral and Biotechnological Sciences, "G. d'Annunzio" University , Chieti, Italy .
authors.forename: Francesca
authors.initials: F
authors.lastname: Diomede
authors.affiliation: 8 Department of Medical, Oral and Biotechnological Sciences, "G. d'Annunzio" University , Chieti, Italy .
authors.forename: Oriana
authors.initials: O
authors.lastname: Trubiani
authors.affiliation: 1 Postgraduate Department, Cruzeiro do Sul University São Paulo , SP, Brasil .
authors.forename: Lucio
authors.initials: L
authors.lastname: Frigo
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000528:radiation effects*; D002478:Cells, Cultured; D004285:Dogs; D006801:Humans; D028022:Low-Level Light Therapy; D059630:Mesenchymal Stem Cells / Q000528:radiation effects*