title: Effect of low-level mechanical vibration on osteogenesis and osseointegration of porous titanium implants in the repair of long bone defects.
abstract: Emerging evidence substantiates the potential of porous titanium alloy (pTi) as an ideal bone-graft substitute because of its excellent biocompatibility and structural properties. However, it remains a major clinical concern for promoting high-efficiency and high-quality osseointegration of pTi, which is beneficial for securing long-term implant stability. Accumulating evidence demonstrates the capacity of low-amplitude whole-body vibration (WBV) in preventing osteopenia, whereas the effects and mechanisms of WBV on osteogenesis and osseointegration of pTi remain unclear. Our present study shows that WBV enhanced cellular attachment and proliferation, and induced well-organized cytoskeleton of primary osteoblasts in pTi. WBV upregulated osteogenesis-associated gene and protein expression in primary osteoblasts, including OCN, Runx2, Wnt3a, Lrp6 and β-catenin. In vivo findings demonstrate that 6-week and 12-week WBV stimulated osseointegration, bone ingrowth and bone formation rate of pTi in rabbit femoral bone defects via μCT, histological and histomorphometric analyses. WBV induced higher ALP, OCN, Runx2, BMP2, Wnt3a, Lrp6 and β-catenin, and lower Sost and RANKL/OPG gene expression in rabbit femora. Our findings demonstrate that WBV promotes osteogenesis and osseointegration of pTi via its anabolic effect and potential anti-catabolic activity, and imply the promising potential of WBV for enhancing the repair efficiency and quality of pTi in osseous defects.
authors.affiliation: Department of Biomedical Engineering, Fourth Military Medical University, Xi'an, China.
authors.forename: Da
authors.initials: D
authors.lastname: Jing
authors.affiliation: Department of Biomedical Engineering, Fourth Military Medical University, Xi'an, China.
authors.forename: Shichao
authors.initials: S
authors.lastname: Tong
authors.affiliation: Department of Biomedical Engineering, Fourth Military Medical University, Xi'an, China.
authors.forename: Mingming
authors.initials: M
authors.lastname: Zhai
authors.affiliation: Institute of Orthopaedics, Xijing hospital, Fourth Military Medical University, Xi'an, China.
authors.forename: Xiaokang
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Endocrinology, Xijing hospital, Fourth Military Medical University, Xi'an, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Cai
authors.affiliation: Institute of Orthopaedics, Xijing hospital, Fourth Military Medical University, Xi'an, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Biomedical Engineering, Fourth Military Medical University, Xi'an, China.
authors.forename: Guanghao
authors.initials: G
authors.lastname: Shen
authors.affiliation: Department of Biomedical Engineering, Fourth Military Medical University, Xi'an, China.
authors.forename: Xuhui
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Nursing, Fourth Military Medical University, Xi'an, China.
authors.forename: Qiaoling
authors.initials: Q
authors.lastname: Xu
authors.affiliation: Institute of Orthopaedics, Xijing hospital, Fourth Military Medical University, Xi'an, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Guo
authors.affiliation: Department of Biomedical Engineering, Fourth Military Medical University, Xi'an, China.
authors.forename: Erping
authors.initials: E
authors.lastname: Luo
mesh_terms: D000497:Alloys / Q000737:chemistry*; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D001847:Bone Diseases / Q000628:therapy; D018786:Bone Substitutes / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D005260:Female; D005269:Femur / Q000000981:diagnostic imaging / Q000378:metabolism / Q000473:pathology; D015870:Gene Expression; D060488:Low Density Lipoprotein Receptor-Related Protein-6 / Q000235:genetics / Q000378:metabolism; D016348:Osseointegration / Q000187:drug effects*; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects*; D016062:Porosity; D019736:Prostheses and Implants; D011817:Rabbits; D011859:Radiography; D014025:Titanium / Q000737:chemistry*; D015854:Up-Regulation / Q000187:drug effects; D014732:Vibration; D051176:beta Catenin / Q000235:genetics / Q000378:metabolism
chemical_list: D000497:Alloys; D001672:Biocompatible Materials; D018786:Bone Substitutes; D060488:Low Density Lipoprotein Receptor-Related Protein-6; D051176:beta Catenin; D014025:Titanium