title: Pulsed electric field improves the bioprotective capacity of purées for different coloured carrot cultivars against H2O2-induced oxidative damage.
abstract: This research aimed to study the effect of pulsed electric field (PEF) processing on the bioprotective capacity of carrot purée for White Belgian, Yellow Solar, Nantes, Nutri Red and Purple Haze cultivars against H2O2-induced oxidative damage. The bioprotective capacity was determined using cell viability, membrane integrity and nitric oxide (NO) production in a human Caco-2 cell culture assay. Total carotenoids, total anthocyanins, total vitamin C and total phenolics were also evaluated. Compared to the untreated purée, Purple Haze and Nutri Red processed at 303 kJ/kg completely increased Caco-2 cells resistance towards oxidative damage by recovering the cell viability and inhibiting NO production. For cultivar with low carotenoid levels, i.e. Yellow Solar, the application of 0.8 kV/cm resulted in a higher total carotenoid content in the purée than its untreated counterpart, leading to an improved bioprotective effect. This study clearly shows that PEF could add value to carrots by maximising bioprotective effects.
authors.affiliation: Department of Food Science, University of Otago, Dunedin, New Zealand; Department of Botany, University of Otago, Dunedin, New Zealand.
authors.forename: Sze Ying
authors.initials: SY
authors.lastname: Leong
authors.affiliation: Department of Food Science, University of Otago, Dunedin, New Zealand. Electronic address: indrawati.oey@otago.ac.nz.
authors.forename: Indrawati
authors.initials: I
authors.lastname: Oey
authors.affiliation: Department of Botany, University of Otago, Dunedin, New Zealand.
authors.forename: David John
authors.initials: DJ
authors.lastname: Burritt
mesh_terms: D000872:Anthocyanins / Q000737:chemistry / Q000378:metabolism; D001205:Ascorbic Acid / Q000737:chemistry / Q000378:metabolism; D018938:Caco-2 Cells; D002338:Carotenoids / Q000737:chemistry / Q000378:metabolism; D002470:Cell Survival; D018552:Daucus carota / Q000737:chemistry* / Q000145:classification / Q000378:metabolism; D004560:Electricity; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism*; D018384:Oxidative Stress; D010636:Phenols / Q000737:chemistry / Q000378:metabolism; D028321:Plant Preparations / Q000737:chemistry / Q000378:metabolism*; D014815:Vitamins / Q000737:chemistry / Q000378:metabolism
chemical_list: D000872:Anthocyanins; D010636:Phenols; D028321:Plant Preparations; D014815:Vitamins; D002338:Carotenoids; D006861:Hydrogen Peroxide; D001205:Ascorbic Acid
keywords: Anthocyanins; Bioprotective capacity; Carotenoids; Carrot; Cell viability; Cultivar; Oxidative stress; Pulsed electric fields