title: Combinatorial Approach to Nanoarchitectonics for Nonviral Delivery of Nucleic Acids.
abstract: Nanoparticles based on cationic polymers, lipids or lipidoids are of great interest in the field of gene delivery applications. The research on these nanosystems is rapidly growing as they hold promise to treat wide variety of human diseases ranging from viral infections to genetic disorders and cancer. Recently, combinatorial design principles have been adopted for rapid generation of large numbers of chemically diverse polymers and lipids capable of forming multifunctional nanocarriers for the use in gene delivery applications. At the same time, current high-throughput screening systems as well as convenient cell assays and readout techniques allow for fast evaluation of cell transfection efficiencies and toxicities of libraries of novel gene delivery agents. This allows for a rapid evaluation of structure-function relationship as well as identification of novel efficient nanocarriers for cell transfection and gene therapy. Here, the recent contribution of high-throughput synthesis to the development of novel nanocarriers for gene delivery applications is described.
authors.affiliation: Institute of Toxicology and Genetics, Karlsruhe Institute of Technology (KIT), Hermann-von-Helmholtz Platz 1, 76344, Eggenstein-Leopoldshafen, Germany.
authors.forename: Mijanur Rahaman
authors.initials: MR
authors.lastname: Molla
authors.affiliation: Institute of Toxicology and Genetics, Karlsruhe Institute of Technology (KIT), Hermann-von-Helmholtz Platz 1, 76344, Eggenstein-Leopoldshafen, Germany.|University of Heidelberg, Department of Applied Physical Chemistry, 69120, Heidelberg, Germany.
authors.forename: Pavel A
authors.initials: PA
authors.lastname: Levkin
mesh_terms: D000818:Animals; D002412:Cations; D020650:Combinatorial Chemistry Techniques; D004247:DNA / Q000737:chemistry; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000737:chemistry; D057809:HEK293 Cells; D006801:Humans; D008055:Lipids / Q000737:chemistry; D051379:Mice; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry; D036103:Nanotechnology / Q000379:methods*; D009696:Nucleic Acids; D011108:Polymers / Q000737:chemistry; D034741:RNA, Small Interfering / Q000378:metabolism; D014162:Transfection
chemical_list: D002412:Cations; D008055:Lipids; D009696:Nucleic Acids; D011108:Polymers; D034741:RNA, Small Interfering; D049452:Green Fluorescent Proteins; D004247:DNA
keywords: cell transfection; combinatorial synthesis; gene delivery; high-throughput; lipids; polymers