title: Screening of HER2 Overexpressed Breast Cancer Subtype In Vivo by the Validation of High-Performance, Long-Term, and Noninvasive Fluorescence Tracer.
abstract: The high-performance and noninvasive screening of heterogeneous tumor subtypes in vivo is particularly desirable for the diagnosis and symptomatic treatment of cancer. Therefore, we report a near-infrared (NIR) fluorescence tracer "smartly identified HER2" (SI-HER2) for rapid, accurate, and highly specific screening of HER2 overexpressed breast cancer. An antibody against HER2 protein receptor, EP1045Y, was conjugated with NIR emitting CdSeTe/CdS/ZnS QDs via polyhistidine-driven self-assembly approach. The further adsorption of black hole quencher 3 on antibody enabled a "turn on" fluorescence response of the fluorescence tracer to HER2 protein receptor. Aside from the capability of differentiating the HER2 overexpressed MCF-7 cells from its counterparts, the fluorescence tracer can also accurately and rapidly identify the HER2 overexpressed breast tumor subtype in two tumors-bearing mouse model, providing a platform for the investigation of advanced pathways to distinguish the different breast cancer subtypes.
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, Collaborative Innovation of Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University , Nanjing 210093, China.|China-America Cancer Research Institute, Guangdong Medical University , Dongguan, Guangdong 523808, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Ding
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, Collaborative Innovation of Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University , Nanjing 210093, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: School of Medical Imaging, Xuzhou Medical College , Xuzhou 221004, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Li
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, Collaborative Innovation of Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University , Nanjing 210093, China.
authors.forename: Liping
authors.initials: L
authors.lastname: Jiang
authors.affiliation: China-America Cancer Research Institute, Guangdong Medical University , Dongguan, Guangdong 523808, China.
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: He
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, Collaborative Innovation of Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University , Nanjing 210093, China.
authors.forename: Jun-Jie
authors.initials: JJ
authors.lastname: Zhu
mesh_terms: D000818:Animals; D001943:Breast Neoplasms / Q000175:diagnosis* / Q000378:metabolism*; D002470:Cell Survival; D004195:Disease Models, Animal; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D061986:MCF-7 Cells; D008325:Mammary Neoplasms, Experimental / Q000175:diagnosis / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D018613:Microscopy, Confocal; D045663:Quantum Dots; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000096:biosynthesis*; D013050:Spectrometry, Fluorescence; D014407:Tumor Cells, Cultured
chemical_list: D018719:Erb-b2 Receptor Tyrosine Kinases; C508053:ERBB2 protein, human