title: Profiling of Brevibacillus borstelensis transcriptome exposed to high temperature shock.
abstract: To understand the molecular mechanisms underlying the ability of the bacteria to survive at high temperature, gene expression profile of Brevibacillusborstelensis at 55°C during 5 and 10min heat shock period was carried out by high-throughput sequencing technology. A total of 2555 non-redundant transcripts were annotated. A total of 575 genes at 5min and 400 genes at 10min exhibited significant differential expression in response to temperature upshift from 50 to 55°C. Genes up-regulated under heat shock were associated with metabolism (mtnE), membrane transport, signal transduction, transcriptional regulation (ycxD, codY) and folding and sorting (hsp90). A larger number of genes encoding hypothetical proteins were identified. RT-PCR experimental results carried out on genes expressed under heat shock were found to be consistent with transcriptome data. The results enhance our understanding of adaptation strategy of thermophilic bacteria thereby providing a strong background for in depth research in thermophiles.
authors.affiliation: Division of Fish Health Management, Central Institute of Freshwater Aquaculture, Kaushalyaganga, Bhubaneswar 751002, Orissa, India.
authors.forename: S
authors.initials: S
authors.lastname: Tripathy
authors.affiliation: Division of Fish Health Management, Central Institute of Freshwater Aquaculture, Kaushalyaganga, Bhubaneswar 751002, Orissa, India.
authors.forename: S K
authors.initials: SK
authors.lastname: Padhi
authors.affiliation: Division of Fish Health Management, Central Institute of Freshwater Aquaculture, Kaushalyaganga, Bhubaneswar 751002, Orissa, India.
authors.forename: R
authors.initials: R
authors.lastname: Sen
authors.affiliation: Division of Fish Health Management, Central Institute of Freshwater Aquaculture, Kaushalyaganga, Bhubaneswar 751002, Orissa, India.
authors.forename: S
authors.initials: S
authors.lastname: Mohanty
authors.affiliation: Division of Fish Health Management, Central Institute of Freshwater Aquaculture, Kaushalyaganga, Bhubaneswar 751002, Orissa, India.
authors.forename: M
authors.initials: M
authors.lastname: Samanta
authors.affiliation: Division of Fish Health Management, Central Institute of Freshwater Aquaculture, Kaushalyaganga, Bhubaneswar 751002, Orissa, India. Electronic address: maitink@yahoo.co.in.
authors.forename: N K
authors.initials: NK
authors.lastname: Maiti
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D058675:Brevibacillus / Q000235:genetics* / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D018841:HSP90 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D018869:Heat-Shock Response; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D059467:Transcriptome
chemical_list: D001426:Bacterial Proteins; D018841:HSP90 Heat-Shock Proteins; D026901:Membrane Transport Proteins; D014157:Transcription Factors
keywords: B. borstelensis; Gene expression profile; Heat shock; RT-PCR