title: Biotechnological promises of Fe-filled CNTs for cell shepherding and magnetic fluid hyperthermia applications.
abstract: Fe-filled carbon nanotubes (Fe@CNTs) recently emerged as an effective class of hybrid nanoparticles for biotechnological applications, such as magnetic cell sorting and magnetic fluid hyperthermia. Aiming at studying the effects of both the Fe loading and the magnetocrystalline characteristics in these applications, we describe herein the preparation of Fe@CNTs containing different Fe phases that, upon functionalization with the antibody Cetuximab (Ctxb), allow the targeting of cancer cells. Our experimental findings reveal that an optimal Ctxb/Fe weight ratio of 1.2 is needed for efficient magnetic cell shepherding, whereas enhanced MFH-induced mortality (70 vs. 15%) can be reached with hybrids enriched in the coercive Fe(3)C phase. These results suggest that a synergistic effect between the Ab loading and the Fe distribution in each nanotube exists, for which the maximum shepherding and hyperthermia effects are observed when higher densities of Fe@CNTs featuring the more coercive phase are interfaced with the cells.
authors.affiliation: Department of Chemistry and Namur Research College (NARC), University of Namur, Rue de Bruxelles 61, Namur, 5000, Belgium. davide.bonifazi@unamur.be.
authors.forename: Florent
authors.initials: F
authors.lastname: Pineux
authors.affiliation: Department of Chemistry and Namur Research College (NARC), University of Namur, Rue de Bruxelles 61, Namur, 5000, Belgium. davide.bonifazi@unamur.be.
authors.forename: Riccardo
authors.initials: R
authors.lastname: Marega
authors.affiliation: Department of Chemistry and Namur Research College (NARC), University of Namur, Rue de Bruxelles 61, Namur, 5000, Belgium. davide.bonifazi@unamur.be.
authors.forename: Antoine
authors.initials: A
authors.lastname: Stopin
authors.affiliation: School of Chemistry, University of Nottingham, University Park, Nottingham, NG7 2RD, UK.
authors.forename: Alessandro
authors.initials: A
authors.lastname: La Torre
authors.affiliation: Institute of Condensed Matter and Nanosciences, Molecules, Solids and Reactivity (IMCN/MOST), Université Catholique de Louvain, Place L. Pasteur 1, Louvain-la-Neuve, 1348, Belgium.
authors.forename: Yann
authors.initials: Y
authors.lastname: Garcia
authors.affiliation: Institute for Advanced Materials, Physicochemical Processes, Nanotechnology and Microsystems, NCSR 'Demokritos', Aghia Paraskevi, 15310 Athens, Greece.
authors.forename: Eamonn
authors.initials: E
authors.lastname: Devlin
authors.affiliation: Unité de Recherche en Biologie Cellulaire (URBC) and NARILIS, University of Namur, Rue de Bruxelles 61, Namur, 5000, Belgium.
authors.forename: Carine
authors.initials: C
authors.lastname: Michiels
authors.affiliation: School of Chemistry, University of Nottingham, University Park, Nottingham, NG7 2RD, UK.
authors.forename: Andrei N
authors.initials: AN
authors.lastname: Khlobystov
authors.affiliation: Department of Chemistry and Namur Research College (NARC), University of Namur, Rue de Bruxelles 61, Namur, 5000, Belgium. davide.bonifazi@unamur.be and Department of Chemical and Pharmaceutical Sciences and INSTM UdR Trieste, University of Trieste, Piazzale Europa, 34127, Trieste, Italy.
authors.forename: Davide
authors.initials: D
authors.lastname: Bonifazi
mesh_terms: D006979:Hyperthermia, Induced; D007501:Iron / Q000737:chemistry* / Q000627:therapeutic use; D055909:Magnetic Field Therapy; D060526:Magnetic Fields; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure
chemical_list: D037742:Nanotubes, Carbon; D007501:Iron