title: Blocking IL-10 signalling at the time of immunization renders the tumour more accessible to T cell infiltration in mice.
abstract: We recently reported that blockade of IL-10 signalling at the time of a human papillomavirus (HPV) long E7 peptide/LPS immunization leads to the regression of established HPV-16 immortalized tumours in mice similar to that induced by long E7 peptide/incomplete Freund's adjuvant (IFA)-based vaccination. In this paper, we demonstrated that blockade of IL-10 signalling at the time of long E7 peptide/LPS could elicit stronger T cells responses and render the tumour more accessible for immune cell infiltration than vaccination with long E7 peptide/IFA. Furthermore, priming with long E7 peptide/LPS and IL10 signalling blockade then boosting with long E7 peptide/IFA elicits stronger CD8+ T cell responses than long E7 peptide/IFA immunization. The results suggest that priming with long E7 peptide/LPS and IL10 signalling inhibitor, then boosting with long E7 peptide/IFA elicits may lead to better HPV infection related tumour regression in clinic.
authors.affiliation: Cancer Research Institute, Foshan First People's Hospital, Foshan, Guangdong 528000, China.
authors.forename: Shu
authors.initials: S
authors.lastname: Chen
authors.affiliation: School of Medical Science, Griffith Health Institute, Griffith University, Gold Coast, QLD 4222, Australia.
authors.forename: Guoying
authors.initials: G
authors.lastname: Ni
authors.affiliation: Cancer Research Institute, Foshan First People's Hospital, Foshan, Guangdong 528000, China.
authors.forename: Xiaolian
authors.initials: X
authors.lastname: Wu
authors.affiliation: Cancer Research Institute, Foshan First People's Hospital, Foshan, Guangdong 528000, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhu
authors.affiliation: Cancer Research Institute, Foshan First People's Hospital, Foshan, Guangdong 528000, China.
authors.forename: Zaowen
authors.initials: Z
authors.lastname: Liao
authors.affiliation: Cancer Research Institute, Foshan First People's Hospital, Foshan, Guangdong 528000, China. Electronic address: wyjian@fsyyy.com.
authors.forename: Yuejian
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Cancer Research Institute, Foshan First People's Hospital, Foshan, Guangdong 528000, China; Inflammation and Healing Research Cluster, School of Health and Sport Sciences, University of Sunshine Coast, Maroochydore DC, QLD 4558, Australia. Electronic address: xiaosongl@yahoo.com.
authors.forename: Xiaosong
authors.initials: X
authors.lastname: Liu
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000818:Animals; D019496:Cancer Vaccines / Q000276:immunology*; D004195:Disease Models, Animal; D058501:Enzyme-Linked Immunospot Assay; D005260:Female; D006801:Humans; D016753:Interleukin-10 / Q000037:antagonists & inhibitors*; D008070:Lipopolysaccharides / Q000276:immunology; D016246:Lymphocytes, Tumor-Infiltrating / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009374:Neoplasms, Experimental / Q000276:immunology* / Q000821:virology*; D050725:Papillomavirus E7 Proteins / Q000276:immunology; D030361:Papillomavirus Infections / Q000150:complications; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D019496:Cancer Vaccines; D008070:Lipopolysaccharides; D050725:Papillomavirus E7 Proteins; C059731:oncogene protein E7, Human papillomavirus type 16; D016753:Interleukin-10
keywords: IL-10 signalling blockade; Immunization; T cell infiltration