title: Osteoinductive peptide-functionalized nanofibers with highly ordered structure as biomimetic scaffolds for bone tissue engineering.
abstract: The construction of functional biomimetic scaffolds that recapitulate the topographical and biochemical features of bone tissue extracellular matrix is now of topical interest in bone tissue engineering. In this study, a novel surface-functionalized electrospun polycaprolactone (PCL) nanofiber scaffold with highly ordered structure was developed to simulate the critical features of native bone tissue via a single step of catechol chemistry. Specially, under slightly alkaline aqueous solution, polydopamine (pDA) was coated on the surface of aligned PCL nanofibers after electrospinning, followed by covalent immobilization of bone morphogenetic protein-7-derived peptides onto the pDA-coated nanofiber surface. Contact angle measurement, Raman spectroscopy, and X-ray photoelectron spectroscopy confirmed the presence of pDA and peptides on PCL nanofiber surface. Our results demonstrated that surface modification with osteoinductive peptides could improve cytocompatibility of nanofibers in terms of cell adhesion, spreading, and proliferation. Most importantly, Alizarin Red S staining, quantitative real-time polymerase chain reaction, immunostaining, and Western blot revealed that human mesenchymal stem cells cultured on aligned nanofibers with osteoinductive peptides exhibited enhanced osteogenic differentiation potential than cells on randomly oriented nanofibers. Furthermore, the aligned nanofibers with osteoinductive peptides could direct osteogenic differentiation of human mesenchymal stem cells even in the absence of osteoinducting factors, suggesting superior osteogenic efficacy of biomimetic design that combines the advantages of osteoinductive peptide signal and highly ordered nanofibers on cell fate decision. The presented peptide-decorated bone-mimic nanofiber scaffolds hold a promising potential in the context of bone tissue engineering.
authors.affiliation: College of Stomatology, Chongqing Medical University, Chongqing, Beijing, People's Republic of China ; Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing Medical University, Chongqing, Beijing, People's Republic of China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Gao
authors.affiliation: Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, People's Republic of China.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Zhang
authors.affiliation: College of Stomatology, Chongqing Medical University, Chongqing, Beijing, People's Republic of China ; Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing Medical University, Chongqing, Beijing, People's Republic of China.
authors.forename: Jinlin
authors.initials: J
authors.lastname: Song
authors.affiliation: Department of Oral and Maxillofacial Surgery, Laboratory of Interdisciplinary Studies, Peking University School and Hospital of Stomatology, Beijing, People's Republic of China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Xu
authors.affiliation: College of Stomatology, Chongqing Medical University, Chongqing, Beijing, People's Republic of China.
authors.forename: Anxiu
authors.initials: A
authors.lastname: Xu
authors.affiliation: Department of Oral and Maxillofacial Surgery, Laboratory of Interdisciplinary Studies, Peking University School and Hospital of Stomatology, Beijing, People's Republic of China.
authors.forename: Mengke
authors.initials: M
authors.lastname: Wang
authors.affiliation: College of Stomatology, Chongqing Medical University, Chongqing, Beijing, People's Republic of China.
authors.forename: Bingwu
authors.initials: B
authors.lastname: Xie
authors.affiliation: Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing Medical University, Chongqing, Beijing, People's Republic of China.
authors.forename: Enyi
authors.initials: E
authors.lastname: Huang
authors.affiliation: College of Stomatology, Chongqing Medical University, Chongqing, Beijing, People's Republic of China ; Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing Medical University, Chongqing, Beijing, People's Republic of China.
authors.forename: Feng
authors.initials: F
authors.lastname: Deng
authors.affiliation: Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing Medical University, Chongqing, Beijing, People's Republic of China ; Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, People's Republic of China ; Department of Oral and Maxillofacial Surgery, Laboratory of Interdisciplinary Studies, Peking University School and Hospital of Stomatology, Beijing, People's Republic of China.
authors.forename: Shicheng
authors.initials: S
authors.lastname: Wei
mesh_terms: D000595:Amino Acid Sequence; D040761:Biomimetic Materials / Q000494:pharmacology*; D001842:Bone and Bones / Q000187:drug effects / Q000502:physiology*; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D022001:Focal Adhesions / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008969:Molecular Sequence Data; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D016348:Osseointegration / Q000187:drug effects*; D010012:Osteogenesis / Q000187:drug effects; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology*; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D010455:Peptides
keywords: biomimetic; bone tissue engineering; nanofiber; peptide