title: Development of neuropeptide Y-mediated heart innervation in rats.
abstract: Neuropeptide Y (NPY) plays a trophic role in the nervous and vascular systems and in cardiac hypertrophy. However, there is no report concerning the expression of NPY and its receptors in the heart during postnatal development. In the current study, immunohistochemistry and Western blot analysis was used to label NPY, and Y1R, Y2R, and Y5R receptors in the heart tissue and intramural cardiac ganglia from rats of different ages (newborn, 10 days old, 20 days old, 30 days old, 60 days old, 1 year old, and 2 years old).The obtained data suggest age-dependent changes of NPY-mediated heart innervation. The density of NPY-immunoreactive (IR) fibers was the least in newborn animals and increased in the first 20 days of life. In the atria of newborn and 10-day-old rats, NPY-IR fibers were more abundant compared with the ventricles. The vast majority of NPY-IR fibers also contained tyrosine hydroxylase, a key enzyme in catecholamine synthesis.The expression of Y1R increased between 10 and 20 days of life. Faint Y2R immunoreactivity was observed in the atria and ventricles of 20-day-old and older rats. In contrast, the highest level of the expression of Y5R was found in newborn pups comparing with more adult rats. All intramural ganglionic neurons were also Y1R-IR and Y5R-IR and Y2R-negative in all studied animals.Thus, the increasing of density of NPY-containing nerve fibers accompanies changes in relation of different subtypes of NPY receptors in the heart during development.
authors.affiliation: Department of Normal Physiology, Yaroslavl State Medical University, ul. Revolucionnaya, 5, Yaroslavl 150000, Russia. Electronic address: mpm@yma.ac.ru.
authors.forename: Petr M
authors.initials: PM
authors.lastname: Masliukov
authors.affiliation: Department of Normal Physiology, Yaroslavl State Medical University, ul. Revolucionnaya, 5, Yaroslavl 150000, Russia.
authors.forename: Konstantin
authors.initials: K
authors.lastname: Moiseev
authors.affiliation: Department of Normal Physiology, Yaroslavl State Medical University, ul. Revolucionnaya, 5, Yaroslavl 150000, Russia.
authors.forename: Andrey I
authors.initials: AI
authors.lastname: Emanuilov
authors.affiliation: Department of Anatomy, Physiology and Healthcare, Kazan (Volga Region) Federal University, Kremlyovskaya St. 18, Kazan 420008, Russia.
authors.forename: Tatyana A
authors.initials: TA
authors.lastname: Anikina
authors.affiliation: Department of Anatomy, Physiology and Healthcare, Kazan (Volga Region) Federal University, Kremlyovskaya St. 18, Kazan 420008, Russia.
authors.forename: Alexey A
authors.initials: AA
authors.lastname: Zverev
authors.affiliation: Department of Physiology, Saint Petersburg State University, Universitetskaya nab., 7-9, Saint-Petersburg 199034, Russia.
authors.forename: Alexandr D
authors.initials: AD
authors.lastname: Nozdrachev
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D005728:Ganglia, Sympathetic / Q000378:metabolism; D006321:Heart / Q000294:innervation*; D007150:Immunohistochemistry / Q000379:methods; D009474:Neurons / Q000378:metabolism; D009478:Neuropeptide Y / Q000378:metabolism*; D017208:Rats, Wistar; D017476:Receptors, Neuropeptide Y / Q000378:metabolism; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
chemical_list: D009478:Neuropeptide Y; D017476:Receptors, Neuropeptide Y; D014446:Tyrosine 3-Monooxygenase
keywords: Autonomic nervous system; Development; Heart innervation; Immunohistochemistry; Intramural ganglia; Neuropeptide Y; Receptors; Western blot analysis