title: Gene Expression and Correlation of Pten and Fabp4 in Liver, Muscle, and Adipose Tissues of Type 2 Diabetes Rats.
abstract: BACKGROUND
  The aim of this work was to study the Fabp4 and Pten gene expression and correlation in the liver, muscle, and adipose tissues of type 2 diabetes mellitus (T2DM) rats.
  
  
  METHODS
  Male Wistar rats (8 weeks old) were randomly divided into 2 groups (n=12/group): a control group fed a normal diet for 8 weeks and an experimental group fed a high-fat, high-sugar diet for 8 weeks and that received 25 mg/kg streptozotocin by intraperitoneal injection to induce T2DM. The random blood glucose, fasting blood glucose, and fasting insulin levels were measured. The expression of Pten and Fabp4 in the liver, muscle, and epididymal adipose tissues was estimated by real-time quantitative PCR. Pearson correlation coefficient analysis was used to investigate the expression correlation between Pten and Fabp4 in T2DM rats.
  
  
  RESULTS
  The gene expressions of Pten and Fabp4 in the liver, muscle, and adipose tissues of T2DM rats were all significantly higher than those in the control group (P<0.05). Pten was highly expressed in the muscles and Fabp4 was highly expressed in muscle and adipose tissues. Furthermore, expressions of Fabp4 and Pten in the muscle and adipose tissues of T2DM rats were positively correlated (P<0.05), but not in the liver.
  
  
  CONCLUSIONS
  The increased expression of PTEN and FABP4 in the adipose and muscles of T2DM rats may play an important role in the insulin resistance of T2DM. However, the mechanism by which these 2 genes function in T2DM needs further study.
authors.affiliation: Department of Endocrinology, The Affiliated Hospital of Inner Mongolia Medical University, Huhhot, Inner Mongolia, China (mainland).
authors.forename: Di
authors.initials: D
authors.lastname: Su
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan, China (mainland).
authors.forename: Chuan-Ling
authors.initials: CL
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Molecular Pathology, Inner Mongolia Medical University, Huhhot, Inner Mongolia, China (mainland).
authors.forename: Ying-Chun
authors.initials: YC
authors.lastname: Gao
authors.affiliation: Department of Gynaecology and Obstetrics, The Affiliated Hospital of Inner Mongolia Medical University, Huhhot, Inner Mongolia, China (mainland).
authors.forename: Xiao-Ying
authors.initials: XY
authors.lastname: Liu
authors.affiliation: Department of Endocrinology, The Affiliated Hospital of Inner Mongolia Medical University, Huhhot, Inner Mongolia, China (mainland).
authors.forename: Cai-Ping
authors.initials: CP
authors.lastname: Li
authors.affiliation: Department of Endocrinology, Affiliated Hospital of Inner Mongolia Medical University, Huhhot, Inner Mongolia, China (mainland).
authors.forename: Jian
authors.initials: J
authors.lastname: Huangfu
authors.affiliation: Key Laboratory of Molecular Pathology, Inner Mongolia Medical University, Huhhot, Inner Mongolia, China (mainland).
authors.forename: Rui
authors.initials: R
authors.lastname: Xiao
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism; D050154:Adiposity; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000235:genetics / Q000378:metabolism; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics* / Q000378:metabolism; D050556:Fatty Acid-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D007328:Insulin / Q000097:blood / Q000378:metabolism; D007333:Insulin Resistance; D008099:Liver / Q000378:metabolism; D008297:Male; D009132:Muscles / Q000378:metabolism; D009765:Obesity / Q000378:metabolism; D051059:PTEN Phosphohydrolase / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
chemical_list: D001786:Blood Glucose; C524662:FABP4 protein, rat; D050556:Fatty Acid-Binding Proteins; D007328:Insulin; D051059:PTEN Phosphohydrolase