title: Estimation of outcrossing rates in interspecific backcross plants of Jatropha curcas (L.) using AFLP and SSR markers.
abstract: In this paper, we report the estimates of outcrossing rates using open-pollinated progeny arrays of 40 BC1 individuals of Jatropha developed as a result of interspecific hybridization between J. curcas and J. integerrima. For analysis PCR-based dominant AFLP and codominant SSR markers were used. The multilocus outcrossing rate (tm) estimated from AFLP markers (0.892 ± 0.112) are almost in the same range with SSR (0.884 ± 0.293) markers which indicate a high level of heterozygosity. A low value of inbreeding coefficient (F) also points out to the fact that outcrossing was the prevalent mode of reproduction in Jatropha and suggests maintenance of adequate genetic variability within families.
authors.affiliation: The Energy and Resources Institute, IHC Complex Lodhi Road, New Delhi, 110 003 India.
authors.forename: Pratima
authors.initials: P
authors.lastname: Sinha
authors.affiliation: The Energy and Resources Institute, IHC Complex Lodhi Road, New Delhi, 110 003 India.
authors.forename: Md Aminul
authors.initials: MA
authors.lastname: Islam
authors.affiliation: The Energy and Resources Institute, IHC Complex Lodhi Road, New Delhi, 110 003 India.
authors.forename: Madan Singh
authors.initials: MS
authors.lastname: Negi
authors.affiliation: The Energy and Resources Institute, IHC Complex Lodhi Road, New Delhi, 110 003 India.
authors.forename: Shashi Bhushan
authors.initials: SB
authors.lastname: Tripathi
keywords: AFLP; Backcross; Biodiesel; Jatropha; Outcrossing; SSR