title: [Identification of C(2)M interacting proteins by yeast two-hybrid screening].
abstract: The synaptonemal complex (SC) is a huge structure which assembles between the homologous chromosomes during meiotic prophase I. Drosophila germ cell-specific nucleoprotein C(2)M clustering at chromosomes can induce SC formation. To further study the molecular function and mechanism of C(2)M in meiosis, we constructed a bait vector for C(2)M and used the yeast two-hybrid system to identify C(2)M interacting proteins. Forty interacting proteins were obtained, including many DNA and histone binding proteins, ATP synthases and transcription factors. Gene silencing assays in Drosophila showed that two genes, wech and Psf1, may delay the disappearance of SC. These results indicate that Wech and Psf1 may form a complex with C(2)M to participate in the formation or stabilization of the SC complex.
authors.affiliation: 1. School of Life Sciences, Anhui University, Anhui 230601, China; 2. State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China;
authors.forename: Shan-shan
authors.initials: SS
authors.lastname: Yue
authors.affiliation: School of Life Sciences, Anhui University, Anhui 230601, China.
authors.forename: Lai-xin
authors.initials: LX
authors.lastname: Xia
mesh_terms: D001483:Base Sequence; D018797:Cell Cycle Proteins / Q000235:genetics / Q000502:physiology*; D019295:Computational Biology; D029721:Drosophila Proteins / Q000235:genetics / Q000502:physiology*; D008969:Molecular Sequence Data; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D013573:Synaptonemal Complex / Q000502:physiology; D020798:Two-Hybrid System Techniques
chemical_list: C499556:C(2)M protein, Drosophila; D018797:Cell Cycle Proteins; D029721:Drosophila Proteins