title: CD4+ T cells from patients with acute myeloid leukemia inhibit the proliferation of bone marrow-derived mesenchymal stem cells by secretion of miR-10a.
abstract: BACKGROUND
  The abnormality of bone marrow-derived mesenchymal stem cells (BM-MSCs) has been reported to contribute to the pathogenesis of acute myeloid leukemia (AML). T cell immunodeficiencies play important roles in the progression of leukemia. This study investigated the effect of CD4+ T cells from AML patients on the proliferation of BM-MSCs.
  
  
  METHODS
  The growth rate of BM-MSCs from AML patients and healthy donor was compared. CD4+ T cells were separated and identified from AML patients. Through co-culturing CD4+ T cells from AML patients and BM-MSCs from healthy, we detected the proliferation of BM-MSCs from healthy by MTT assay. qRT-PCR was performed to examine the expression of miR-10a. Luciferase reporter assay was used to analyze the regulation of miR-10a on the expression of BCL6.
  
  
  RESULTS
  Here, we observed that BM-MSC from AML patients grew slower than that from healthy. CD4+ T cells from AML patients inhibited the proliferation of BM-MSCs through secreting miR-10a. In addition, miR-10a was found to target BCL6 and regulated its expression in transcription and translation levels. Correlation analysis revealed that the level of miR-10a in serum of AML patients was negatively correlated with BCL6 in BM-MSC.
  
  
  CONCLUSIONS
  This study provides evidence that CD4+ T cells from AML patients suppress the proliferation of BM-MSCs via secreting miR-10a.
authors.affiliation: Department of Pediatrics, Qilu Hospital, Shandong University, 107 Wen Hua Xi Road, Jinan, Shandong, 250012, People's Republic of China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Yu
authors.affiliation: Department of Pediatrics, Qilu Hospital, Shandong University, 107 Wen Hua Xi Road, Jinan, Shandong, 250012, People's Republic of China.
authors.forename: Dong
authors.initials: D
authors.lastname: Li
authors.affiliation: Department of Pediatrics, Qilu Hospital, Shandong University, 107 Wen Hua Xi Road, Jinan, Shandong, 250012, People's Republic of China. jxiuli2015@163.com.
authors.forename: Xiu-li
authors.initials: XL
authors.lastname: Ju
mesh_terms: D000328:Adult; D000368:Aged; D015153:Blotting, Western; D001854:Bone Marrow Cells / Q000378:metabolism*; D015496:CD4-Positive T-Lymphocytes; D002454:Cell Differentiation; D049109:Cell Proliferation; D018920:Coculture Techniques; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005260:Female; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D008297:Male; D059630:Mesenchymal Stem Cells; D035683:MicroRNAs / Q000378:metabolism*; D008875:Middle Aged; D051560:Proto-Oncogene Proteins c-bcl-6; D060888:Real-Time Polymerase Chain Reaction; D014162:Transfection
chemical_list: C084103:BCL6 protein, human; D004268:DNA-Binding Proteins; C547183:MIRN10 microRNA, human; D035683:MicroRNAs; D051560:Proto-Oncogene Proteins c-bcl-6
keywords: Acute myeloid leukemia; BCL6; Bone marrow mesenchymal stromal cells; Proliferation; T cells; miR-10a