title: Multidisciplinary assessment of pesticide mitigation in soil amended with vermicomposted agroindustrial wastes.
abstract: Soil organic amendment affects biotic and abiotic processes that control the fate of pesticides, but the treatment history of the soil is also relevant. These processes were assessed in a multidisciplinary study with the aim of optimizing pesticide mitigation in soils. Soil microcosms pre-treated (E2) or not with diuron (E1) were amended with either winery (W) or olive waste (O) vermicomposts. Herbicide dissipation followed a double first-order model in E1 microcosms, but a single first-order model in E2. Also, diuron persistence was longer in E1 than in E2 (E1-DT50>200 day(-1), E2-DT50<16 day(-1)). The genetic structure of the bacterial community was modified by both diuron exposure and amendment. O-vermicompost increased enzymatic activities in both experiments, but diuron-degrading genetic potential (puhB) was quantified only in E2 microcosms in accordance with reduced diuron persistence. Therefore, O-vermicompost addition favoured the proliferation of diuron degraders, increasing the soil diuron-depuration capability.
authors.affiliation: Department of Environmental Protection, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Cientificas (EEZ-CSIC), C/Profesor Albareda 1, 18008 Granada, Spain. Electronic address: jeanmanuel.castillo04@gmail.com.
authors.forename: Jean Manuel
authors.initials: JM
authors.lastname: Castillo
authors.affiliation: French National Institute for Agricultural Research-INRA, UMR 1347 Agroécologie, 17 rue Sully, B P 86510, 21065 Dijon Cedex, France.
authors.forename: Jérèmie
authors.initials: J
authors.lastname: Beguet
authors.affiliation: French National Institute for Agricultural Research-INRA, UMR 1347 Agroécologie, 17 rue Sully, B P 86510, 21065 Dijon Cedex, France.
authors.forename: Fabrice
authors.initials: F
authors.lastname: Martin-Laurent
authors.affiliation: Department of Environmental Protection, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Cientificas (EEZ-CSIC), C/Profesor Albareda 1, 18008 Granada, Spain.
authors.forename: Esperanza
authors.initials: E
authors.lastname: Romero
mesh_terms: D000327:Adsorption; D001426:Bacterial Proteins / Q000235:genetics; D004237:Diuron / Q000737:chemistry / Q000378:metabolism; D019649:Food Industry; D005798:Genes, Bacterial / Q000235:genetics; D007220:Industrial Waste; D000069463:Olive Oil; D010088:Oxidoreductases / Q000378:metabolism; D010575:Pesticides / Q000737:chemistry / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012987:Soil; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000737:chemistry / Q000378:metabolism; D014510:Urease / Q000378:metabolism; D014920:Wine
chemical_list: D001426:Bacterial Proteins; D007220:Industrial Waste; D000069463:Olive Oil; D010575:Pesticides; D012336:RNA, Ribosomal, 16S; D012987:Soil; D012989:Soil Pollutants; D004237:Diuron; D010088:Oxidoreductases; D014510:Urease
keywords: ARISA fingerprinting; Biodegradation; Diuron; Phenylurea hydrolase genes; Vermicompost