title: PDL-1/PDL-2 blockade in mice dendritic cells by RNAi techniques to induce antitumor immunity.
abstract: OBJECTIVE
  We provided potent dendritic cells (DCs) for induction of stronger antitumor immune responses.
  
  
  METHODS
  Using siRNA and shRNA systems, PDL-1 and PDL-2 were knocked down and then DC in vitro and in vivo properties were evaluated.
  
  
  RESULTS
  Mild suppression of PDL-1/PDL-2 molecules was accompanied by appropriate expression of DCs co-stimulatory molecules and release of proinflammatory cytokines. In vitro T-cell engagement induced the proliferation and secretion of Th1 cytokines. Injection of DCs to a 4T1 mice model induced intratumor CD8(+) infiltrating lymphocytes, splenocytes expansion, Th1 cytokine profile shift, and a mild drift to tumor growth inhibition and mice survival.
  
  
  CONCLUSIONS
  Manipulated DCs induced significant antitumor immunity, but this subject needs further evaluation in different animals.
authors.affiliation: Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
authors.forename: Saeed
authors.initials: S
authors.lastname: Daneshmandi
authors.affiliation: Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
authors.forename: Ali Akbar
authors.initials: AA
authors.lastname: Pourfathollah
authors.affiliation: Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Mohammad Hossein
authors.initials: MH
authors.lastname: Karimi
authors.affiliation: Department of Genetics, Shahrekord University, Shahrekord, Iran.|Research institute of Biotechnology, Shahrekord University, Shahrekord, Iran.
authors.forename: Modjtaba
authors.initials: M
authors.lastname: Emadi-Baygi
mesh_terms: D000818:Animals; D060890:B7-H1 Antigen / Q000235:genetics / Q000378:metabolism*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002277:Carcinoma / Q000628:therapy*; D045744:Cell Line, Tumor; D002465:Cell Movement; D003713:Dendritic Cells / Q000502:physiology* / Q000637:transplantation; D005260:Female; D015316:Genetic Therapy; D057809:HEK293 Cells; D006801:Humans; D016219:Immunotherapy, Adoptive / Q000379:methods*; D008213:Lymphocyte Activation / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009374:Neoplasms, Experimental; D061027:Programmed Cell Death 1 Ligand 2 Protein / Q000235:genetics / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000235:genetics; D018417:Th1 Cells / Q000276:immunology*; D047368:Tumor Burden; D019139:Tumor Escape / Q000235:genetics
chemical_list: D060890:B7-H1 Antigen; D061027:Programmed Cell Death 1 Ligand 2 Protein; D034741:RNA, Small Interfering
keywords: PDL-1; PDL-2; dendritic cell; shRNA; siRNA