title: The biofilm property and its correlationship with high-molecular-weight polyacrylamide degradation in a water injection pipeline of Daqing oilfield.
abstract: Biofilms increase dragging force for liquid transportation, cause power consumption, and result in equipment corrosion in polymer-flooding oilfields. To reveal the responsible microorganisms for biofilm formation and stability of high-molecular-weight polyacrylamide (PAM), a biofilm, developed on the sieve of a piston plunger pump in a water transport and injection pipeline with partial hydrolyzed polyacrylamide (HPAM) in Daqing Oilfield, was collected and analyzed by molecular microbiology, chemical and physical methods. Diverse bacterial groups (11 families) were detected in the biofilm, including Pseudomonadaceae, Rhodocyclaceae, Desulfobulbaceae, Alcaligenaceae, Comamonadaceae, Oxalobacteraceae, Bacteriovoracaceae, Campylobacteraceae, Flavobacteriaceae, Clostridiales Incertae Sedis XIII and Moraxellaceae. Three archaeal orders of methanogens including Methanomicrobiales, Methanosarcinales and Thermoplasmatales were also detected separately. HPAM was degraded into lower molecular weight polymers and organic fragments with its amide groups hydrolyzed into carboxylic groups by the microorganisms. The microenvironment of the biofilm contained diverse bacterial and archaeal communities, correlating with the extracellular polymeric substance (EPS) and HPAM biodegradation. The results are helpful to provide information for biofilm control in oil fields.
authors.affiliation: State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, PR China. Electronic address: licaiyun@mail.ecust.edu.cn.
authors.forename: Cai-Yun
authors.initials: CY
authors.lastname: Li
authors.affiliation: The fourth oil production Company of Daqing Oilfield Limited Company, PetroChina, Daqing 163511, Heilongjiang, PR China. Electronic address: dzhang@petrochina.com.cn.
authors.forename: Dong
authors.initials: D
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, PR China. Electronic address: Lixiaoxiao@mail.ecust.edu.cn.
authors.forename: Xiao-Xiao
authors.initials: XX
authors.lastname: Li
authors.affiliation: State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, PR China. Electronic address: smmbadinga@ecust.edu.cn.
authors.forename: Serge Maurice
authors.initials: SM
authors.lastname: Mbadinga
authors.affiliation: State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, PR China. Electronic address: meor@ecust.edu.cn.
authors.forename: Shi-Zhong
authors.initials: SZ
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, PR China. Electronic address: ljf@ecust.edu.cn.
authors.forename: Jin-Feng
authors.initials: JF
authors.lastname: Liu
authors.affiliation: School of Biological Sciences, The University of Hong Kong, Pokfulam Road, Hong Kong SAR, PR China. Electronic address: jdgu@hku.hk.
authors.forename: Ji-Dong
authors.initials: JD
authors.lastname: Gu
authors.affiliation: State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, PR China; Shanghai Collaborative Innovation Center of Biomanufacturing Technology, Shanghai 200237, PR China. Electronic address: bzmu@ecust.edu.cn.
authors.forename: Bo-Zhong
authors.initials: BZ
authors.lastname: Mu
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry / Q000378:metabolism*; D001105:Archaea / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D001673:Biodegradation, Environmental; D018441:Biofilms; D001704:Biopolymers / Q000378:metabolism; D008970:Molecular Weight; D061329:Oil and Gas Fields; D019642:RNA, Archaeal / Q000235:genetics; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D014867:Water; D000099166:Polyacrylamides
chemical_list: D000180:Acrylic Resins; D001704:Biopolymers; D019642:RNA, Archaeal; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D014867:Water; D000099166:Polyacrylamides
keywords: Biodegradation; Biofilm; EPS; Microbial diversity; Polyacrylamide