title: Transcriptome Analysis of Syringa oblata Lindl. Inflorescence Identifies Genes Associated with Pigment Biosynthesis and Scent Metabolism.
abstract: Syringa oblata Lindl. is a woody ornamental plant with high economic value and characteristics that include early flowering, multiple flower colors, and strong fragrance. Despite a long history of cultivation, the genetics and molecular biology of S. oblata are poorly understood. Transcriptome and expression profiling data are needed to identify genes and to better understand the biological mechanisms of floral pigments and scents in this species. Nine cDNA libraries were obtained from three replicates of three developmental stages: inflorescence with enlarged flower buds not protruded, inflorescence with corolla lobes not displayed, and inflorescence with flowers fully opened and emitting strong fragrance. Using the Illumina RNA-Seq technique, 319,425,972 clean reads were obtained and were assembled into 104,691 final unigenes (average length of 853 bp), 41.75% of which were annotated in the NCBI non-redundant protein database. Among the annotated unigenes, 36,967 were assigned to gene ontology categories and 19,956 were assigned to eukaryoticorthologous groups. Using the Kyoto Encyclopedia of Genes and Genomes pathway database, 12,388 unigenes were sorted into 286 pathways. Based on these transcriptomic data, we obtained a large number of candidate genes that were differentially expressed at different flower stages and that were related to floral pigment biosynthesis and fragrance metabolism. This comprehensive transcriptomic analysis provides fundamental information on the genes and pathways involved in flower secondary metabolism and development in S. oblata, providing a useful database for further research on S. oblata and other plants of genus Syringa.
authors.affiliation: College of Landscape Architecture, Beijing University of Agriculture, Beijing, 102206, China.|Beijing Engineering Research Center of rural landscape planning and design, Beijing, 102206, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zheng
authors.affiliation: College of Landscape Architecture, Beijing University of Agriculture, Beijing, 102206, China.
authors.forename: Zenghui
authors.initials: Z
authors.lastname: Hu
authors.affiliation: College of Landscape Architecture, Beijing University of Agriculture, Beijing, 102206, China.
authors.forename: Xuelian
authors.initials: X
authors.lastname: Guan
authors.affiliation: College of Landscape Architecture, Beijing University of Agriculture, Beijing, 102206, China.
authors.forename: Dequan
authors.initials: D
authors.lastname: Dou
authors.affiliation: College of Landscape Architecture, Beijing University of Agriculture, Beijing, 102206, China.
authors.forename: Guo
authors.initials: G
authors.lastname: Bai
authors.affiliation: College of Landscape Architecture, Beijing University of Agriculture, Beijing, 102206, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Landscape Architecture, Beijing University of Agriculture, Beijing, 102206, China.
authors.forename: Yingtian
authors.initials: Y
authors.lastname: Guo
authors.affiliation: College of Landscape Architecture and Forestry, Qingdao Agricultural University, Qingdao, 266100, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: College of Landscape Architecture, Beijing University of Agriculture, Beijing, 102206, China.
authors.forename: Pingsheng
authors.initials: P
authors.lastname: Leng
mesh_terms: D035264:Flowers / Q000235:genetics* / Q000254:growth & development; D018506:Gene Expression Regulation, Plant; D059014:High-Throughput Nucleotide Sequencing; D058977:Molecular Sequence Annotation; D010940:Plant Proteins / Q000096:biosynthesis* / Q000235:genetics; D031662:Syringa / Q000235:genetics* / Q000254:growth & development; D059467:Transcriptome / Q000235:genetics*
chemical_list: D010940:Plant Proteins