title: MicroRNA-125b suppresses the epithelial-mesenchymal transition and cell invasion by targeting ITGA9 in melanoma.
abstract: Increasing evidence has shown that aberrant miRNAs contribute to the development and progression of human melanoma. Previous studies have shown that miR-125b functions as a suppressor in malignant melanoma. However, the molecular function and mechanism by which miR-125b influences melanoma growth and invasion are still unclear. In this study, we aimed to investigate the role of miR-125b in melanoma progression and metastasis. We found that miR-125b expression is significantly downregulated in primary melanoma, and an even greater downregulation was observed in metastatic invasion. Restored expression of miR-125b in melanoma suppressed cell proliferation and invasion both in vitro and in vivo. Furthermore, our findings demonstrate that upregulating miR-125b significantly inhibits malignant phenotypes by repressing the expression of integrin alpha9 (ITGA9). Finally, our data reveal that upregulated expression of ITGA9 in melanoma tissues is inversely associated with miR-125b levels. Together, our results demonstrate that upregulation of ITGA9 in response to the decrease in miR-125b in metastatic melanoma is responsible for melanoma tumor cell migration and invasion.
authors.affiliation: Department of Plastic Surgery, The First Affiliated Hospital to Nanchang University, No.17 Yongwaizheng Street, Nanchang, Jiangxi, 330006, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Oral and Maxillofacial Surgery, The First Affiliated Hospital to Nanchang University, Nanchang, Jiangxi, 330006, China.
authors.forename: Sijia
authors.initials: S
authors.lastname: Na
authors.affiliation: Department of Plastic Surgery, Changzheng Hospital, Second Military Medical University, Shanghai, 200003, China.
authors.forename: Caiyue
authors.initials: C
authors.lastname: Liu
authors.affiliation: Department of Oral and Maxillofacial Surgery, The First Affiliated Hospital to Nanchang University, Nanchang, Jiangxi, 330006, China.
authors.forename: Shuting
authors.initials: S
authors.lastname: Pan
authors.affiliation: Department of Anesthesiology, The Second Affiliated Hospital to Nanchang University, Nanchang, Jiangxi, 330006, China.
authors.forename: Junying
authors.initials: J
authors.lastname: Cai
authors.affiliation: Department of Oral and Maxillofacial Surgery, The First Affiliated Hospital to Nanchang University, Nanchang, Jiangxi, 330006, China. qjxdoctor@163.com.
authors.forename: Jiaxuan
authors.initials: J
authors.lastname: Qiu
mesh_terms: D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002448:Cell Adhesion / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D004195:Disease Models, Animal; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D016023:Integrins / Q000737:chemistry / Q000235:genetics*; D008545:Melanoma / Q000235:genetics* / Q000473:pathology*; D051379:Mice; D035683:MicroRNAs / Q000737:chemistry / Q000235:genetics*; D009362:Neoplasm Metastasis; D034622:RNA Interference; D023041:Xenograft Model Antitumor Assays
chemical_list: C541973:ITGA9 protein, human; D016023:Integrins; C531888:MIRN125 microRNA, human; D035683:MicroRNAs
keywords: EMT; ITGA9; Invasion; Melanoma; miR-125b