title: Ultrasound assisted intensification of biodiesel production using enzymatic interesterification.
abstract: Ultrasound assisted intensification of synthesis of biodiesel from waste cooking oil using methyl acetate and immobilized lipase obtained from Thermomyces lanuginosus (Lipozyme TLIM) as a catalyst has been investigated in the present work. The reaction has also been investigated using the conventional approach based on stirring so as to establish the beneficial effects obtained due to the use of ultrasound. Effect of operating conditions such as reactant molar ratio (oil and methyl acetate), temperature and enzyme loading on the yield of biodiesel has been investigated. Optimum conditions for the conventional approach (without ultrasound) were established as reactant molar ratio of 1:12 (oil:methyl acetate), enzyme loading of 6% (w/v), temperature of 40 °C and reaction time of 24 h and under these conditions, 90.1% biodiesel yield was obtained. The optimum conditions for the ultrasound assisted approach were oil to methyl acetate molar ratio of 1:9, enzyme loading of 3% (w/v), and reaction time of 3 h and the biodiesel yield obtained under these conditions was 96.1%. Use of ultrasound resulted in significant reduction in the reaction time with higher yields and lower requirement of the enzyme loading. The obtained results have clearly established that ultrasound assisted interesterification was a fast and efficient approach for biodiesel production giving significant benefits, which can help in reducing the costs of production. Reusability studies for the enzyme were also performed but it was observed that reuse of the catalyst under the optimum experimental condition resulted in reduced enzyme activity and biodiesel yield.
authors.affiliation: Chemical Engineering Department, Institute of Chemical Technology, Matunga, Mumbai 400 019, India.
authors.forename: Preeti B
authors.initials: PB
authors.lastname: Subhedar
authors.affiliation: Chemical Engineering Department, Institute of Chemical Technology, Matunga, Mumbai 400 019, India. Electronic address: pr.gogate@ictmumbai.edu.in.
authors.forename: Parag R
authors.initials: PR
authors.lastname: Gogate
mesh_terms: D056804:Biofuels; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D004951:Esterification; D032641:Eurotiales / Q000201:enzymology; D007700:Kinetics; D008049:Lipase / Q000737:chemistry / Q000378:metabolism*; D013499:Surface Properties; D013696:Temperature; D000069453:Ultrasonic Waves
chemical_list: D056804:Biofuels; D004800:Enzymes, Immobilized; D008049:Lipase
keywords: Biodiesel; Immobilized lipase; Intensification; Interesterification; Operating parameters; Ultrasound