title: Mobility Measurements Probe Conformational Changes in Membrane Proteins due to Tension.
abstract: The function of membrane-embedded proteins such as ion channels depends crucially on their conformation. We demonstrate how conformational changes in asymmetric membrane proteins may be inferred from measurements of their diffusion. Such proteins cause local deformations in the membrane, which induce an extra hydrodynamic drag on the protein. Using membrane tension to control the magnitude of the deformations, and hence the drag, measurements of diffusivity can be used to infer-via an elastic model of the protein-how conformation is changed by tension. Motivated by recent experimental results [Quemeneur et al., Proc. Natl. Acad. Sci. U.S.A. 111, 5083 (2014)], we focus on KvAP, a voltage-gated potassium channel from Aeropyrum pernix. The conformation of KvAP is found to change considerably due to tension, with its "walls," where the protein meets the membrane, undergoing significant angular strains. The torsional stiffness is determined to be 26.8k(B)T per radian at room temperature. This has implications for both the structure and the function of such proteins in the environment of a tension-bearing membrane.
authors.affiliation: Department of Physics and Centre for Complexity Science, University of Warwick, Coventry CV4 7AL, United Kingdom.
authors.forename: Richard G
authors.initials: RG
authors.lastname: Morris
authors.affiliation: Department of Physics and Centre for Complexity Science, University of Warwick, Coventry CV4 7AL, United Kingdom.
authors.forename: Matthew S
authors.initials: MS
authors.lastname: Turner
mesh_terms: D041522:Aeropyrum / Q000737:chemistry / Q000378:metabolism; D008956:Models, Chemical; D015336:Molecular Probe Techniques; D015335:Molecular Probes / Q000737:chemistry; D024642:Potassium Channels, Voltage-Gated / Q000737:chemistry* / Q000378:metabolism; D011487:Protein Conformation; D013816:Thermodynamics; D019415:Torque
chemical_list: D015335:Molecular Probes; D024642:Potassium Channels, Voltage-Gated