title: [On the Effect of α-Tocopherol on Protein Kinase C Activity in vitro].
abstract: The effect of the antioxidant α-tocopherol on rat brain protein kinase C activity as a model to study bimodal dose-dependent effect has been investigated. Enzyme activity has been monitored photometrically with a luciferase reporter assay that measures ADP produced by posphorylation. The inhibition of protein kinase C activity by α-tocopherol was found at the concentration range from 10(-3) to 10(-6) M with no effect of ultra low doses of the antioxidant (below. 10(-12) M). The absence of bimodal dose-dependent effect may be associated with the enzyme source.
authors.forename: N E
authors.initials: NE
authors.lastname: Krassova
authors.forename: S V
authors.initials: SV
authors.lastname: Ugraitskaya
authors.forename: N V
authors.initials: NV
authors.lastname: Penkov
authors.forename: E E
authors.initials: EE
authors.lastname: Fesenko
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage*; D001921:Brain / Q000187:drug effects / Q000201:enzymology; D009131:Muscle, Smooth, Vascular / Q000378:metabolism; D010766:Phosphorylation; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D051381:Rats; D024502:alpha-Tocopherol / Q000008:administration & dosage*
chemical_list: D000975:Antioxidants; D011493:Protein Kinase C; D024502:alpha-Tocopherol