title: Confocal multiview light-sheet microscopy.
abstract: Selective-plane illumination microscopy has proven to be a powerful imaging technique due to its unsurpassed acquisition speed and gentle optical sectioning. However, even in the case of multiview imaging techniques that illuminate and image the sample from multiple directions, light scattering inside tissues often severely impairs image contrast. Here we combine multiview light-sheet imaging with electronic confocal slit detection implemented on modern camera sensors. In addition to improved imaging quality, the electronic confocal slit detection doubles the acquisition speed in multiview setups with two opposing illumination directions allowing simultaneous dual-sided illumination. Confocal multiview light-sheet microscopy eliminates the need for specimen-specific data fusion algorithms, streamlines image post-processing, easing data handling and storage.
authors.affiliation: European Molecular Biology Laboratory, Cell Biology and Biophysics Unit, Meyerhofstrasse 1, 69117, Heidelberg, Germany.
authors.forename: Gustavo
authors.initials: G
authors.lastname: de Medeiros
authors.affiliation: European Molecular Biology Laboratory, Cell Biology and Biophysics Unit, Meyerhofstrasse 1, 69117, Heidelberg, Germany.|European Molecular Biology Laboratory, Developmental Biology Unit, Meyerhofstrasse 1, 69117, Heidelberg, Germany.
authors.forename: Nils
authors.initials: N
authors.lastname: Norlin
authors.affiliation: European Molecular Biology Laboratory, Cell Biology and Biophysics Unit, Meyerhofstrasse 1, 69117, Heidelberg, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Gunther
authors.affiliation: European Molecular Biology Laboratory, Developmental Biology Unit, Meyerhofstrasse 1, 69117, Heidelberg, Germany.
authors.forename: Marvin
authors.initials: M
authors.lastname: Albert
authors.affiliation: European Molecular Biology Laboratory, Developmental Biology Unit, Meyerhofstrasse 1, 69117, Heidelberg, Germany.
authors.forename: Laura
authors.initials: L
authors.lastname: Panavaite
authors.affiliation: European Molecular Biology Laboratory, Cell Biology and Biophysics Unit, Meyerhofstrasse 1, 69117, Heidelberg, Germany.
authors.forename: Ulla-Maj
authors.initials: UM
authors.lastname: Fiuza
authors.affiliation: European Molecular Biology Laboratory, Developmental Biology Unit, Meyerhofstrasse 1, 69117, Heidelberg, Germany.
authors.forename: Francesca
authors.initials: F
authors.lastname: Peri
authors.affiliation: European Molecular Biology Laboratory, Developmental Biology Unit, Meyerhofstrasse 1, 69117, Heidelberg, Germany.
authors.forename: Takashi
authors.initials: T
authors.lastname: Hiiragi
authors.affiliation: European Molecular Biology Laboratory, Cell Biology and Biophysics Unit, Meyerhofstrasse 1, 69117, Heidelberg, Germany.
authors.forename: Uros
authors.initials: U
authors.lastname: Krzic
authors.affiliation: European Molecular Biology Laboratory, Cell Biology and Biophysics Unit, Meyerhofstrasse 1, 69117, Heidelberg, Germany.
authors.forename: Lars
authors.initials: L
authors.lastname: Hufnagel