title: Immunophenotype of mouse cerebral hemispheres-derived neural precursor cells.
abstract: Postnatally isolated neural precursor cells (piNPCs) from mouse cerebral tissue have been studied in cell-based therapeutic approaches for Experimental Autoimmune Encephalomyelitis (EAE). Transplantation experiments in EAE rodents revealed that piNPCs manage to integrate into the host tissue and ameliorate clinical symptoms. When cultured in vitro, mouse cerebral piNPCs form neurospheres consisting of immature cells positive for polysialylated neural adhesion molecule (PSA-NCAM) that differentiate mainly towards glial cells, but also neurons. Herein, we have characterized piNPCs immunophenotype, with flow cytometry. NPCs were positive for CD24, CD44, and CD133 though negative for CD15, CD184 and CD49d. This immunophenotype, determined for the first time, among cells isolated from neonates might be useful for the identification of NPC population aiming at the development of transplantation protocols.
authors.affiliation: Laboratory of Experimental Neurology and Neuroimmunology, B' Department of Neurology, AHEPA University Hospital Aristotle University, Thessaloniki 54636, Greece.
authors.forename: Kyriaki-Nefeli
authors.initials: KN
authors.lastname: Poulatsidou
authors.affiliation: Laboratory of Experimental Neurology and Neuroimmunology, B' Department of Neurology, AHEPA University Hospital Aristotle University, Thessaloniki 54636, Greece.
authors.forename: Roza
authors.initials: R
authors.lastname: Lagoudaki
authors.affiliation: Laboratory of Experimental Neurology and Neuroimmunology, B' Department of Neurology, AHEPA University Hospital Aristotle University, Thessaloniki 54636, Greece.
authors.forename: Olga
authors.initials: O
authors.lastname: Touloumi
authors.affiliation: Laboratory of Experimental Neurology and Neuroimmunology, B' Department of Neurology, AHEPA University Hospital Aristotle University, Thessaloniki 54636, Greece.
authors.forename: Evangelia
authors.initials: E
authors.lastname: Kesidou
authors.affiliation: Laboratory of Experimental Neurology and Neuroimmunology, B' Department of Neurology, AHEPA University Hospital Aristotle University, Thessaloniki 54636, Greece.
authors.forename: Marina
authors.initials: M
authors.lastname: Boziki
authors.affiliation: Hasselt University, Biomedical Research Institute/Transnational University Limburg, School of Life Sciences, Diepenbeek 3590, Belgium.
authors.forename: Stylianos
authors.initials: S
authors.lastname: Ravanidis
authors.affiliation: Department of Molecular Biology & Genetics, Democritus University of Thrace, Alexandroupolis 68100, Greece.
authors.forename: Katerina
authors.initials: K
authors.lastname: Chlichlia
authors.affiliation: Department of Molecular Biology & Genetics, Democritus University of Thrace, Alexandroupolis 68100, Greece.
authors.forename: Maria
authors.initials: M
authors.lastname: Grigoriou
authors.affiliation: Laboratory of Experimental Neurology and Neuroimmunology, B' Department of Neurology, AHEPA University Hospital Aristotle University, Thessaloniki 54636, Greece. Electronic address: grigoria@med.auth.gr.
authors.forename: Nikolaos
authors.initials: N
authors.lastname: Grigoriadis
mesh_terms: D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D000954:Antigens, Surface / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D001921:Brain / Q000166:cytology*; D002454:Cell Differentiation; D002478:Cells, Cultured; D016130:Immunophenotyping; D008810:Mice, Inbred C57BL; D039902:Multipotent Stem Cells / Q000166:cytology / Q000276:immunology; D058953:Neural Stem Cells / Q000166:cytology / Q000276:immunology*
chemical_list: D000954:Antigens, Surface; D015415:Biomarkers
keywords: CD molecules; Flow cytometry; Mouse neural precursor cells; Polysialylated neural adhesion molecule