title: Integrating the selection of PHA storing biomass and nitrogen removal via nitrite in the main wastewater treatment line.
abstract: A novel scheme was developed for the treatment of municipal wastewater integrating nitritation/denitritation with the selection of polyhydroxyalkanoates (PHA) storing biomass under an aerobic/anoxic, feast/famine regime. The process took place in a sequencing batch reactor (SBR) and the subsequent PHA accumulation in a batch reactor. The carbon source added during the selection and accumulation steps consisted of fermentation liquid from the organic fraction of municipal solids waste (OFMSW FL) (Period I) and OFMSW and primary sludge fermentation liquid (Period II). Selection of PHA storing biomass was successful and denitritation was driven by internally stored PHA during the famine phase. Under optimum conditions of SBR operation ammonia removal was 93%, reaching a maximum nitrite removal of 98%. The treated effluent met the nitrogen limits, while PHA-storing biomass was successfully selected. The maximum accumulation of PHA was 10.6% (wt.) since the nutrients present in the carbon source promoted bacterial growth.
authors.affiliation: Department of Chemical Engineering, University of Barcelona, C/Martí i Franquès, 1-11, 08028 Barcelona, Spain. Electronic address: nuria.basset@ub.edu.
authors.forename: N
authors.initials: N
authors.lastname: Basset
authors.affiliation: Department of Mechanical, Aerospace and Civil Engineering, Brunel University, Kingston Lane, UB8 3PH Uxbridge, Middlesex, UK.
authors.forename: E
authors.initials: E
authors.lastname: Katsou
authors.affiliation: Department of Biotechnology, University of Verona, Strada Le Grazie 15, 37134 Verona, Italy.
authors.forename: N
authors.initials: N
authors.lastname: Frison
authors.affiliation: Department of Water Resources and Environmental Engineering, School of Civil Engineering, National Technical University of Athens, 5 Iroon Polytechniou St., 15780 Athens, Greece.
authors.forename: S
authors.initials: S
authors.lastname: Malamis
authors.affiliation: Department of Chemical Engineering, University of Barcelona, C/Martí i Franquès, 1-11, 08028 Barcelona, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Dosta
authors.affiliation: Department of Biotechnology, University of Verona, Strada Le Grazie 15, 37134 Verona, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Fatone
mesh_terms: D064751:Ammonium Compounds / Q000032:analysis; D061249:Batch Cell Culture Techniques; D001673:Biodegradation, Environmental / Q000187:drug effects; D018533:Biomass; D001704:Biopolymers / Q000302:isolation & purification; D019149:Bioreactors / Q000382:microbiology; D002244:Carbon / Q000494:pharmacology; D005232:Fatty Acids, Volatile / Q000032:analysis; D005285:Fermentation / Q000187:drug effects; D009573:Nitrites / Q000302:isolation & purification*; D009584:Nitrogen / Q000302:isolation & purification*; D010100:Oxygen / Q000378:metabolism; D054813:Polyhydroxyalkanoates / Q000378:metabolism*; D012722:Sewage / Q000382:microbiology; D014865:Waste Disposal, Fluid; D062065:Wastewater / Q000737:chemistry*; D018508:Water Purification / Q000379:methods*
chemical_list: D064751:Ammonium Compounds; D001704:Biopolymers; D002244:Carbon; D005232:Fatty Acids, Volatile; D009573:Nitrites; D009584:Nitrogen; D010100:Oxygen; D054813:Polyhydroxyalkanoates; D012722:Sewage; D062065:Wastewater
keywords: Feast/famine; Fermentation liquid; Nitritation/denitritation; Nitrogen removal; Polyhydroxyalkanoates