title: Mitochondrial Respiration Chain Enzymatic Activities in the Human Brain: Methodological Implications for Tissue Sampling and Storage.
abstract: Mitochondrial respiratory chain complexes enzymatic (MRCCE) activities were successfully evaluated in frozen brain samples. Epilepsy surgery offers an ethical opportunity to study human brain tissue surgically removed to treat drug resistant epilepsies. Epilepsy surgeries are done with hemodynamic and laboratory parameters to maintain physiology, but there are no studies analyzing the association among these parameters and MRCCE activities in the human brain tissue. We determined the intra-operative parameters independently associated with MRCCE activities in middle temporal neocortex (Cx), amygdala (AMY) and head of hippocampus (HIP) samples of patients (n = 23) who underwent temporal lobectomy using multiple linear regressions. MRCCE activities in Cx, AMY and HIP are differentially associated to trans-operative mean arterial blood pressure, O2 saturation, hemoglobin, and anesthesia duration to time of tissue sampling. The time-course between the last seizure occurrence and tissue sampling as well as the sample storage to biochemical assessments were also associated with enzyme activities. Linear regression models including these variables explain 13-17 % of MRCCE activities and show a moderate to strong effect (r = 0.37-0.82). Intraoperative hemodynamic and laboratory parameters as well as the time from last seizure to tissue sampling and storage time are associated with MRCCE activities in human samples from the Cx, AMYG and HIP. Careful control of these parameters is required to minimize confounding biases in studies using human brain samples collected from elective neurosurgery.
authors.affiliation: Centro de Neurociências Aplicadas, Hospital Universitário (HU), Universidade Federal de Santa Catarina (UFSC), Florianópolis, SC, Brazil.
authors.forename: Marcelo Fernando
authors.initials: MF
authors.lastname: Ronsoni
authors.affiliation: Laboratório de Bioenergética e Estresse Oxidativo, Departamento de Bioquímica, Universidade Federal de Santa Catarina (UFSC), Florianópolis, SC, Brazil.
authors.forename: Aline Pertile
authors.initials: AP
authors.lastname: Remor
authors.affiliation: Laboratório de Transdução de Sinal no Sistema Nervoso Central, Departamento de Bioquímica, Universidade Federal de Santa Catarina (UFSC), Florianópolis, SC, Brazil.
authors.forename: Mark William
authors.initials: MW
authors.lastname: Lopes
authors.affiliation: Centro de Neurociências Aplicadas, Hospital Universitário (HU), Universidade Federal de Santa Catarina (UFSC), Florianópolis, SC, Brazil.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Hohl
authors.affiliation: Centro de Neurociências Aplicadas, Hospital Universitário (HU), Universidade Federal de Santa Catarina (UFSC), Florianópolis, SC, Brazil.
authors.forename: Iris H Z
authors.initials: IH
authors.lastname: Troncoso
authors.affiliation: Centro de Neurociências Aplicadas, Hospital Universitário (HU), Universidade Federal de Santa Catarina (UFSC), Florianópolis, SC, Brazil.|Laboratório de Transdução de Sinal no Sistema Nervoso Central, Departamento de Bioquímica, Universidade Federal de Santa Catarina (UFSC), Florianópolis, SC, Brazil.
authors.forename: Rodrigo Bainy
authors.initials: RB
authors.lastname: Leal
authors.affiliation: Centro de Ensino e Treinamento Integrado de Anestesiologia, Hospital Governador Celso Ramos (HGCR), Florianópolis, SC, Brazil.
authors.forename: Gustavo Luchi
authors.initials: GL
authors.lastname: Boos
authors.affiliation: Centro de Neurociências Aplicadas, Hospital Universitário (HU), Universidade Federal de Santa Catarina (UFSC), Florianópolis, SC, Brazil.|Divisão de Neurocirurgia, Departamento de Cirurgia, Hospital Universitário (HU), Universidade Federal de Santa Catarina (UFSC), Florianópolis, SC, Brazil.
authors.forename: Charles
authors.initials: C
authors.lastname: Kondageski
authors.affiliation: Centro de Neurociências Aplicadas, Hospital Universitário (HU), Universidade Federal de Santa Catarina (UFSC), Florianópolis, SC, Brazil.|Laboratório de Neuropatologia, Serviço de Patologia, Hospital Universitário (HU), Universidade Federal de Santa Catarina (UFSC), Florianópolis, SC, Brazil.
authors.forename: Jean Costa
authors.initials: JC
authors.lastname: Nunes
authors.affiliation: Centro de Neurociências Aplicadas, Hospital Universitário (HU), Universidade Federal de Santa Catarina (UFSC), Florianópolis, SC, Brazil.|Serviço de Cirurgia de Epilepsia, Hospital Governador Celso Ramos (HGCR), Florianópolis, SC, Brazil.
authors.forename: Marcelo Neves
authors.initials: MN
authors.lastname: Linhares
authors.affiliation: Centro de Neurociências Aplicadas, Hospital Universitário (HU), Universidade Federal de Santa Catarina (UFSC), Florianópolis, SC, Brazil.|Serviço de Neurologia, Departamento de Clínica Médica, Hospital Universitário, 3 andar, Universidade Federal de Santa Catarina (UFSC), Florianópolis, SC, 88.040-970, Brazil.
authors.forename: Kátia
authors.initials: K
authors.lastname: Lin
authors.affiliation: Centro de Neurociências Aplicadas, Hospital Universitário (HU), Universidade Federal de Santa Catarina (UFSC), Florianópolis, SC, Brazil.|Laboratório de Bioenergética e Estresse Oxidativo, Departamento de Bioquímica, Universidade Federal de Santa Catarina (UFSC), Florianópolis, SC, Brazil.
authors.forename: Alexandra Susana
authors.initials: AS
authors.lastname: Latini
authors.affiliation: Centro de Neurociências Aplicadas, Hospital Universitário (HU), Universidade Federal de Santa Catarina (UFSC), Florianópolis, SC, Brazil. rogerwalz@hotmail.com.|Serviço de Neurologia, Departamento de Clínica Médica, Hospital Universitário, 3 andar, Universidade Federal de Santa Catarina (UFSC), Florianópolis, SC, 88.040-970, Brazil. rogerwalz@hotmail.com.
authors.forename: Roger
authors.initials: R
authors.lastname: Walz
mesh_terms: D000328:Adult; D038481:Anterior Temporal Lobectomy; D001921:Brain / Q000201:enzymology* / Q000473:pathology / Q000601:surgery; D042967:Electron Transport Complex I / Q000378:metabolism*; D042963:Electron Transport Complex II / Q000378:metabolism*; D003576:Electron Transport Complex IV / Q000378:metabolism*; D004827:Epilepsy / Q000201:enzymology* / Q000473:pathology / Q000601:surgery; D005260:Female; D005615:Freezing; D006801:Humans; D008297:Male; D013048:Specimen Handling / Q000379:methods; D013385:Succinate Dehydrogenase / Q000378:metabolism
chemical_list: D042963:Electron Transport Complex II; D013385:Succinate Dehydrogenase; D003576:Electron Transport Complex IV; D042967:Electron Transport Complex I
keywords: Amygdala; Hippocampus; Mitochondria respiration; Predictor model