title: Variation in extracellular matrix genes is associated with weight regain after weight loss in a sex-specific manner.
abstract: The extracellular matrix (ECM) of adipocytes is important for body weight regulation. Here, we investigated whether genetic variation in ECM-related genes is associated with weight regain among participants of the European DiOGenes study. Overweight and obese subjects (n = 469, 310 females, 159 males) were on an 8-week low-calorie diet with a 6-month follow-up. Body weight was measured before and after the diet, and after follow-up. Weight maintenance scores (WMS, regained weight as percentage of lost weight) were calculated based on the weight data. Genotype data were retrieved for 2903 SNPs corresponding to 124 ECM-related genes. Regression analyses provided us with six significant SNPs associated with the WMS in males: 3 SNPs in the POSTN gene and a SNP in the LAMB1, COL23A1, and FBLN5 genes. For females, 1 SNP was found in the FN1 gene. The risk of weight regain was increased by: the C/C genotype for POSTN in a co-dominant model (OR 8.25, 95 % CI 2.85-23.88) and the T/C-C/C genotype in a dominant model (OR 4.88, 95 % CI 2.35-10.16); the A/A genotype for LAMB1 both in a co-dominant model (OR 18.43, 95 % CI 2.35-144.63) and in a recessive model (OR 16.36, 95 % CI 2.14-124.9); the G/A genotype for COL23A1 in a co-dominant model (OR 3.94, 95 % CI 1.28-12.10), or the A-allele in a dominant model (OR 2.86, 95 % CI 1.10-7.49); the A/A genotype for FBLN5 in a co-dominant model (OR 13.00, 95 % CI 1.61-104.81); and the A/A genotype for FN1 in a recessive model (OR 2.81, 95 % CI 1.40-5.63). Concluding, variants of ECM genes are associated with weight regain after weight loss in a sex-specific manner.
authors.affiliation: Department of Human Biology, NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University, Universiteitssingel 40, 6200 MD, Maastricht, The Netherlands. n.roumans@maastrichtuniversity.nl.
authors.forename: Nadia J T
authors.initials: NJ
authors.lastname: Roumans
authors.affiliation: Department of Human Biology, NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University, Universiteitssingel 40, 6200 MD, Maastricht, The Netherlands.
authors.forename: Roel G
authors.initials: RG
authors.lastname: Vink
authors.affiliation: Department of Complex Genetics, NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University, Universiteitssingel 40, 6200 MD, Maastricht, The Netherlands.
authors.forename: Marij
authors.initials: M
authors.lastname: Gielen
authors.affiliation: Department of Complex Genetics, NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University, Universiteitssingel 40, 6200 MD, Maastricht, The Netherlands.
authors.forename: Maurice P
authors.initials: MP
authors.lastname: Zeegers
authors.affiliation: Centre for Health and Society, Institute of Preventive Medicine, Copenhagen University Hospital, Hovedvejen 5, Frederiksberg, 2000, Copenhagen, Denmark.
authors.forename: Claus
authors.initials: C
authors.lastname: Holst
authors.affiliation: Department of Human Biology, NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University, Universiteitssingel 40, 6200 MD, Maastricht, The Netherlands.|Laboratory of Biochemical Genetics, Department of Clinical Genetics, Maastricht University Medical Centre, University Hospital Maastricht, P. Debyelaan 25, 6229 HX, Maastricht, The Netherlands.
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.affiliation: Department of Nutrition, Exercise and Sports, Faculty of Science, University of Copenhagen, Nørre Allé 51, 2200, Copenhagen, Copenhagen, Denmark.
authors.forename: Arne
authors.initials: A
authors.lastname: Astrup
authors.affiliation: Department of Human Biology, NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University, Universiteitssingel 40, 6200 MD, Maastricht, The Netherlands.
authors.forename: Wim H
authors.initials: WH
authors.lastname: Saris
authors.affiliation: Nestlé Institute of Health Sciences, EPFL Innovation Park, 1015, Lausanne, Switzerland.
authors.forename: Armand
authors.initials: A
authors.lastname: Valsesia
authors.affiliation: Nestlé Institute of Health Sciences, EPFL Innovation Park, 1015, Lausanne, Switzerland.
authors.forename: Jörg
authors.initials: J
authors.lastname: Hager
authors.affiliation: Department of Human Biology, NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University, Universiteitssingel 40, 6200 MD, Maastricht, The Netherlands.
authors.forename: Marleen A
authors.initials: MA
authors.lastname: van Baak
authors.affiliation: Department of Human Biology, NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University, Universiteitssingel 40, 6200 MD, Maastricht, The Netherlands.
authors.forename: Edwin C M
authors.initials: EC
authors.lastname: Mariman
keywords: Adipocytes; Extracellular matrix; SNPs; Weight regain