title: In vitro evaluation of anti-diabetic activity and cytotoxicity of chemically analysed Ocimum basilicum extracts.
abstract: The aim of this study was to evaluate the role of glucose transporter-4 (GLUT4) in the anti-diabetic effects of methanol, hexane and dichloromethane extracts of the aerial parts of Ocimum basilicum (OB) and to analyze their phytochemical composition. Phytochemical analysis of the three extracts by GC/MS using the silylation derivatization technique revealed 53 compounds, 17 of them were found for the first time in OB. Cytotoxic and anti-diabetic properties of the extracts were evaluated using L6-GLUT4myc muscle cells stably expressing myc epitope at the exofacial loop (GLUT4). No cytotoxic effects were observed in treated cells up to 0.25 mg/ml extract as measured with MTT and LDH-leakage assays. GLUT4 translocation to the plasma membrane was elevated by 3.5 and 7 folds (-/+ insulin) after treatment with OB extracts for 20 h. Our findings suggest that the observed anti-diabetic properties of OB extracts are possibly mediated in part through one or more of the 17 new identified compound.
authors.affiliation: Qasemi Research Center - Al-Qasemi Academy, P.O. Box 124, Baqa El-Gharbia 30100, Israel; Casali Center for Applied Chemistry, Institute of Chemistry, The Hebrew University of Jerusalem, Edmond J. Safra Campus, Jerusalem 91904, Israel.
authors.forename: Sleman
authors.initials: S
authors.lastname: Kadan
authors.affiliation: Qasemi Research Center - Al-Qasemi Academy, P.O. Box 124, Baqa El-Gharbia 30100, Israel; Faculty of Arts and Sciences, Arab American University Jenin, P.O. Box 240, Jenin, Palestine. Electronic address: bashar@qsm.ac.il.
authors.forename: Bashar
authors.initials: B
authors.lastname: Saad
authors.affiliation: Casali Center for Applied Chemistry, Institute of Chemistry, The Hebrew University of Jerusalem, Edmond J. Safra Campus, Jerusalem 91904, Israel.
authors.forename: Yoel
authors.initials: Y
authors.lastname: Sasson
authors.affiliation: Qasemi Research Center - Al-Qasemi Academy, P.O. Box 124, Baqa El-Gharbia 30100, Israel; Faculty of Arts and Sciences, Arab American University Jenin, P.O. Box 240, Jenin, Palestine. Electronic address: hilalz@qsm.ac.il.
authors.forename: Hilal
authors.initials: H
authors.lastname: Zaid
mesh_terms: D003920:Diabetes Mellitus / Q000188:drug therapy*; D051275:Glucose Transporter Type 4; D006801:Humans; D066298:In Vitro Techniques; D007328:Insulin / Q000378:metabolism*; D008958:Models, Molecular; D018649:Ocimum basilicum / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*
chemical_list: D051275:Glucose Transporter Type 4; D007328:Insulin; D010936:Plant Extracts; C495692:SLC2A4 protein, human
keywords: Chemical composition; GC/MS; GLUT4; Ocimum basilicum