title: Attenuation of quorum sensing-mediated virulence of Acinetobacter baumannii by Glycyrrhiza glabra flavonoids.
abstract: OBJECTIVE
  To develop an alternative quorum quenching therapy against multidrug-resistant Acinetobacter baumannii.
  
  
  RESULTS
  Activity-guided partially purified fraction (F1) from Glycyrrhiza glabra significantly (p < 0.05) reduced quorum sensing regulated virulence factors of A. baumannii viz. motility, biofilm formation and production of antioxidant enzymes. Mechanistically, F1 downregulated the expression of autoinducer synthase gene, abaI, and consequently reduced (92%) the production of 3-OH-C12-HSL as determined by ESI-MS. Q-TOF and Q-TRAP analyses suggested the presence of flavonoids viz. licoricone, glycyrin and glyzarin as the active ingredients.
  
  
  CONCLUSIONS
  This is the first report on quorum quenching activity of G. glabra linked to its flavonoids that downregulated the expression of abaI and attenuated quorum sensing regulated virulence of A. baumannii.
authors.affiliation: Department of Biotechnology, Panjab University, Chandigarh 160014, India.
authors.forename: Nidhi
authors.initials: N
authors.lastname: Bhargava
authors.affiliation: NSW Department of Primary Industries, Central Coast Primary Industries Centre, Locked Bag 26, Gosford NSW 2250, Australia.
authors.forename: Sukhvinder P
authors.initials: SP
authors.lastname: Singh
authors.affiliation: University Institute of Pharmaceutical Sciences, Panjab University, Chandigarh 160014, India.
authors.forename: Anupam
authors.initials: A
authors.lastname: Sharma
authors.affiliation: Department of Microbiology, Panjab University, Chandigarh 160014, India.
authors.forename: Prince
authors.initials: P
authors.lastname: Sharma
authors.affiliation: Department of Biotechnology, Panjab University, Chandigarh 160014, India.
authors.forename: Neena
authors.initials: N
authors.lastname: Capalash
mesh_terms: D040981:Acinetobacter baumannii / Q000187:drug effects* / Q000502:physiology; D000900:Anti-Bacterial Agents / Q000302:isolation & purification* / Q000494:pharmacology*; D005419:Flavonoids / Q000302:isolation & purification* / Q000494:pharmacology*; D006035:Glycyrrhiza / Q000737:chemistry*; D053038:Quorum Sensing / Q000187:drug effects*; D014774:Virulence / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D005419:Flavonoids
keywords: Acinetobacter baumannii; Glycyrrhiza glabra; biofilm; catalase; flavonoids; motility; quorum sensing inhibition; superoxide dismutase