title: Association of CYP1A1 A4889G and T6235C polymorphisms with the risk of sporadic breast cancer in Brazilian women.
abstract: OBJECTIVE
  We examined the influence of CYP1A1 A4889G and T6235C polymorphisms on the risk of sporadic breast cancer.
  
  
  METHODS
  DNA from 742 sporadic breast cancer patients and 742 controls was analyzed using the polymerase chain reaction, followed by the restriction fragment length polymorphism technique.
  
  
  RESULTS
  More patients had the CYP1A1 4889AG+GG genotype compared to controls (29.0% versus 23.2%, p=0.004). The G allele carriers had a 1.50-fold increased risk (95% CI: 1.14-1.97) of sporadic breast cancer compared to the other study participants. The frequency of the 4889AG+GG genotype among the Caucasian patients was higher than in the non-Caucasian patients (30.4% versus 20.2%, p=0.03) and controls (30.4% versus 23.2%, p=0.002). Caucasians and G allele carriers had a 1.61-fold increased risk (95% CI: 1.20-2.15) of sporadic breast cancer compared to other subjects. The CYP1A1 4889AG+GG genotype was more common among patients with a younger median age at first full-term pregnancy than among controls (33.8% versus 23.2%, p=0.001) and subjects whose first full-term pregnancies occurred at an older age (33.8% versus 26.1%, p=0.03). Women with the CYP1A1 4889AG+GG genotype and earlier first full-term pregnancies had a 1.87-fold (95% CI: 1.32-2.67) increased risk of sporadic breast cancer compared to the other study participants. Excess CYP1A1 4889AG+GG (39.8% versus27.1%, p=0.01) and 6235TC+CC (48.4% versus 35.9%, p=0.02) genotypes were also observed in patients with grade I and II tumors compared to patients with grade III tumors and controls (39.8% versus 23.2%, p=0.04; 48.4% versus 38.6%, p=0.04). The G and C allele carriers had a 2.44-fold (95% CI: 1.48-4.02) and 1.67-fold (95% CI: 1.03-2.69) increased risk, respectively, of developing grade I and II tumors compared to other subjects.
  
  
  CONCLUSIONS
  The CYP1A1 A4889G and T6235C polymorphisms may alter the risk of sporadic breast cancer in Brazilian women.
authors.affiliation: Departamento de Clênica Médica, Faculdade de Ciências Médicas, Universidade de Campinas, Campinas, SP, Brazil.
authors.forename: Camila Borges Martins de
authors.initials: CB
authors.lastname: Oliveira
authors.affiliation: Centro de Assistência Integral è Saúde da Mulher, Universidade de Campinas, Campinas, SP, Brazil.
authors.forename: Cássio
authors.initials: C
authors.lastname: Cardoso-Filho
authors.affiliation: Centro de Assistência Integral è Saúde da Mulher, Universidade de Campinas, Campinas, SP, Brazil.
authors.forename: Leonardo Silveira
authors.initials: LS
authors.lastname: Bossi
authors.affiliation: Departamento de Clênica Médica, Faculdade de Ciências Médicas, Universidade de Campinas, Campinas, SP, Brazil.
authors.forename: Gustavo Jacob
authors.initials: GJ
authors.lastname: Lourenço
authors.affiliation: Centro de Assistência Integral è Saúde da Mulher, Universidade de Campinas, Campinas, SP, Brazil.
authors.forename: Maria Salete
authors.initials: MS
authors.lastname: Costa-Gurgel
authors.affiliation: Departamento de Clênica Médica, Faculdade de Ciências Médicas, Universidade de Campinas, Campinas, SP, Brazil.
authors.forename: Carmen Silvia Passos
authors.initials: CS
authors.lastname: Lima
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001938:Brazil; D001943:Breast Neoplasms / Q000235:genetics*; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics*; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D011247:Pregnancy; D012307:Risk Factors; D055815:Young Adult
chemical_list: D019363:Cytochrome P-450 CYP1A1