title: Double-Layer Magnetic Nanoparticle-Embedded Silica Particles for Efficient Bio-Separation.
abstract: Superparamagnetic Fe3O4 nanoparticles (NPs) based nanomaterials have been exploited in various biotechnology fields including biomolecule separation. However, slow accumulation of Fe3O4 NPs by magnets may limit broad applications of Fe3O4 NP-based nanomaterials. In this study, we report fabrication of Fe3O4 NPs double-layered silica nanoparticles (DL MNPs) with a silica core and highly packed Fe3O4 NPs layers. The DL MNPs had a superparamagnetic property and efficient accumulation kinetics under an external magnetic field. Moreover, the magnetic field-exposed DL MNPs show quantitative accumulation, whereas Fe3O4 NPs single-layered silica nanoparticles (SL MNPs) and silica-coated Fe3O4 NPs produced a saturated plateau under full recovery of the NPs. DL MNPs are promising nanomaterials with great potential to separate and analyze biomolecules.
authors.affiliation: School of Chemical and Biological Engineering, Seoul National University, Seoul, Korea.
authors.forename: San
authors.initials: S
authors.lastname: Kyeong
authors.affiliation: School of Chemical and Biological Engineering, Seoul National University, Seoul, Korea.
authors.forename: Cheolhwan
authors.initials: C
authors.lastname: Jeong
authors.affiliation: Nano Systems Institute and Interdisciplinary Program in Nano-Science and Technology, Seoul National University, Seoul, Korea.
authors.forename: Homan
authors.initials: H
authors.lastname: Kang
authors.affiliation: Center for Theragnosis, Korea Institute of Science and Technology, Seoul, Korea.
authors.forename: Hong-Jun
authors.initials: HJ
authors.lastname: Cho
authors.affiliation: School of Chemical and Biological Engineering, Seoul National University, Seoul, Korea.
authors.forename: Sung-Jun
authors.initials: SJ
authors.lastname: Park
authors.affiliation: School of Chemical and Biological Engineering, Seoul National University, Seoul, Korea.
authors.forename: Jin-Kyoung
authors.initials: JK
authors.lastname: Yang
authors.affiliation: Center for Theragnosis, Korea Institute of Science and Technology, Seoul, Korea.
authors.forename: Sehoon
authors.initials: S
authors.lastname: Kim
authors.affiliation: Department of Bioscience and Biotechnology, Konkuk University, Seoul, Korea.
authors.forename: Hyung-Mo
authors.initials: HM
authors.lastname: Kim
authors.affiliation: Department of Bioscience and Biotechnology, Konkuk University, Seoul, Korea.
authors.forename: Bong-Hyun
authors.initials: BH
authors.lastname: Jun
authors.affiliation: School of Chemical and Biological Engineering, Seoul National University, Seoul, Korea.
authors.forename: Yoon-Sik
authors.initials: YS
authors.lastname: Lee
mesh_terms: D052203:Ferrosoferric Oxide / Q000737:chemistry*; D007700:Kinetics; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D058185:Magnetite Nanoparticles; D012822:Silicon Dioxide; D052203:Ferrosoferric Oxide