title: Evaluation of Oxidative Damage and Antioxidant Mechanisms in COPD, Lung Cancer, and Obstructive Sleep Apnea Syndrome.
abstract: BACKGROUND
  Oxidative damage is a major contributing factor to carcinogenesis and obstructive disorders in lungs. Current evidence suggests that the inflammatory processes yield to oxidative mechanisms, which underlie COPD, lung cancer, and obstructive sleep apnea syndrome (OSAS). This study aimed to evaluate the oxidative damage in these diseases by evaluating the oxidative and antioxidant biomarkers.
  
  
  METHODS
  Malondialdehyde, 8-oxo-7,8-dihydro-2'-deoxyguanosine, and coenzyme Q10 levels were evaluated in the blood samples of subjects with COPD, lung cancer, and OSAS by high-pressure liquid chromatography.
  
  
  RESULTS
  A total of 111 participants (35 females, 76 males) with OSAS (n = 29), COPD (n = 26), and lung cancer (n = 28) and healthy controls (n = 28) were included in the study. The malondialdehyde and coenzyme Q10 levels were significantly higher in all 3 diseases when compared with controls (P < .01), whereas 8-oxo-7,8-dihydro-2'-deoxyguanosine levels were only significantly higher than in healthy controls in subjects with lung cancer (P = .005). The highest levels of malondialdehyde and coenzyme Q10 were determined in subjects with OSAS and lung cancer, respectively. The highest 8-oxo-7,8-dihydro-2'-deoxyguanosine levels were also observed in subjects with lung cancer, but the differences of this biomarker with other diagnoses were not statistically significant (P = .56).
  
  
  CONCLUSIONS
  Oxidative damage was observed in all 3 diagnoses, and, as a response to oxidative stress, antioxidant mechanisms were also active in these diseases. Malondialdehyde and 8-oxo-7,8-dihydro-2'-deoxyguanosine were found to be efficiently usable in the evaluation of oxidative damage in chronic respiratory diseases. (ClinicalTrials.gov registration NCT02406053.).
authors.affiliation: Department of Chest Diseases, Yuzuncu Yil University Medical Faculty.
authors.forename: Aysel
authors.initials: A
authors.lastname: Sunnetcioglu
authors.affiliation: Department of Biochemistry, School of Medicine, Yuzuncu Yil University, Van, Turkey.
authors.forename: Hamit H
authors.initials: HH
authors.lastname: Alp
authors.affiliation: Department of Chest Diseases, Yuzuncu Yil University Medical Faculty.
authors.forename: Bunyamin
authors.initials: B
authors.lastname: Sertogullarından
authors.affiliation: Department of Biochemistry, School of Medicine, Yuzuncu Yil University, Van, Turkey.
authors.forename: Ragip
authors.initials: R
authors.lastname: Balaharoglu
authors.affiliation: Department of Chest Diseases, Yuzuncu Yil University Medical Faculty.
authors.forename: Hulya
authors.initials: H
authors.lastname: Gunbatar
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000368:Aged; D000975:Antioxidants / Q000378:metabolism*; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000097:blood; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000097:blood*; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008875:Middle Aged; D018384:Oxidative Stress; D011446:Prospective Studies; D029424:Pulmonary Disease, Chronic Obstructive / Q000097:blood*; D020181:Sleep Apnea, Obstructive / Q000097:blood*; D014451:Ubiquinone / Q000031:analogs & derivatives / Q000097:blood
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D014451:Ubiquinone; D008315:Malondialdehyde; D000080242:8-Hydroxy-2'-Deoxyguanosine; C024989:coenzyme Q10; D003849:Deoxyguanosine
keywords: 8-oxo-7,8-dihydro-2′-deoxyguanosine; COPD; OSAS; antioxidants; coenzyme Q10; lung cancer; malondialdehyde; oxidative damage