title: Enhancement of naphthalene tolerance in transgenic Arabidopsis plants overexpressing the ferredoxin-like protein (ADI1) from rice.
abstract: The ADI1 Arabidopsis plants enhanced tolerance and degradation efficiency to naphthalene and had great potential for phytoremediation of naphthalene in the plant material before composting or harvesting and removal. Naphthalene is a global environmental concern, because this substance is assumed to contribute considerably to human cancer risk. Cleaning up naphthalene contamination in the environment is crucial. Phytoremediation is an efficient technology to clean up contaminants. However, no gene that can efficiently degrade exogenous recalcitrant naphthalene in plants has yet been discovered. Ferredoxin (Fd) is a key player of biological electron transfer reaction in the PAH degradation process. The biochemical pathway for bacterial degradation of naphthalene has been well investigated. In this study, a rice gene, ADI1, which codes for a putative photosynthetic-type Fd, has been transformed into Arabidopsis thaliana. The transgenic Arabidopsis plants enhanced tolerance and degradation efficiency of naphthalene. Compared with wild-type plants, transgenic plants assimilated naphthalene from the culture media faster and removed more of this substance. When taken together, our findings suggest that breeding plants with overexpressed ADI1 gene is an effective strategy to degrade naphthalene in the environment.
authors.affiliation: Shanghai Key Laboratory of Agricultural Genetics and Breeding, Agro-Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, 2901 Beidi Road, Shanghai, 201106, People's Republic of China.
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Fu
authors.affiliation: Shanghai Key Laboratory of Agricultural Genetics and Breeding, Agro-Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, 2901 Beidi Road, Shanghai, 201106, People's Republic of China.
authors.forename: Bo
authors.initials: B
authors.lastname: Zhu
authors.affiliation: Shanghai Key Laboratory of Agricultural Genetics and Breeding, Agro-Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, 2901 Beidi Road, Shanghai, 201106, People's Republic of China.
authors.forename: Hong-Juan
authors.initials: HJ
authors.lastname: Han
authors.affiliation: Shanghai Key Laboratory of Agricultural Genetics and Breeding, Agro-Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, 2901 Beidi Road, Shanghai, 201106, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhao
authors.affiliation: Shanghai Key Laboratory of Agricultural Genetics and Breeding, Agro-Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, 2901 Beidi Road, Shanghai, 201106, People's Republic of China.
authors.forename: Yong-Sheng
authors.initials: YS
authors.lastname: Tian
authors.affiliation: Shanghai Key Laboratory of Agricultural Genetics and Breeding, Agro-Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, 2901 Beidi Road, Shanghai, 201106, People's Republic of China. pengrihe69@yahoo.com.
authors.forename: Ri-He
authors.initials: RH
authors.lastname: Peng
authors.affiliation: Shanghai Key Laboratory of Agricultural Genetics and Breeding, Agro-Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, 2901 Beidi Road, Shanghai, 201106, People's Republic of China. yaoquanhong_sh@yahoo.com.cn.
authors.forename: Quan-Hong
authors.initials: QH
authors.lastname: Yao
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000502:physiology*; D001673:Biodegradation, Environmental; D005288:Ferredoxins / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D009281:Naphthalenes / Q000378:metabolism*; D012275:Oryza / Q000235:genetics*; D010788:Photosynthesis; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified
chemical_list: D005288:Ferredoxins; D009281:Naphthalenes; D010940:Plant Proteins; C031721:naphthalene
keywords: ADI1 gene; Naphthalene; Phytoremediation; Transgenic Arabidopsis