title: [The effect of estrogen on adipogenic differentiation of rat bone mesenchymal stem cells].
abstract: OBJECTIVE
  In this study, 10⁻⁹ mol/L 17 β-estradiol (E2) was applied in the adipogenic differentiation of rat bone mesenchymal stem cells (rBMSCs) and the effect of E2 was explored.
  
  
  METHODS
  Rat BMSCs were obtained from the femurs and tibias of SD rats. 10⁻⁹ mol/L E2 was involved in the adipogenic differentiation of rBMSCs. Oil red staining, real time PCR and Western blot were carried out to detect the effect of 10⁻⁹ mol/L E2 on adipogenic differentiation of rBMSCs. The data was statistically analyzed using SPSS 19.0 software package.
  
  
  RESULTS
  The use of 10⁻⁹ mol/L E2 decreased the amount of lipid droplets in rBMSCs and weakened the expression of adipogenic related genes and proteins like C/EBP α, C/EBP β, PPAR γ, aP2, and ARDP, which were significantly lower than the adipogenic induced group.
  
  
  CONCLUSIONS
  The use of 10⁻⁹ mol/L E2 inhibited adipogenic differentiation of rBMSCs significantly in vitro.
authors.affiliation: Department of Oral and Craniomaxillofacial Science, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine; Shanghai Key Lab of Stomatology. Shanghai 200011, China.E-mail:jadewong19881113@hotmail.com.
authors.forename: Jie
authors.initials: J
authors.lastname: Wang
authors.forename: Ning-juan
authors.initials: NJ
authors.lastname: Ouyang
authors.forename: Peng
authors.initials: P
authors.lastname: Zhang
authors.forename: Qing-gang
authors.initials: QG
authors.lastname: Dai
authors.forename: Bing
authors.initials: B
authors.lastname: Fang
authors.forename: Ling-yong
authors.initials: LY
authors.lastname: Jiang
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells; D002454:Cell Differentiation; D002478:Cells, Cultured; D004958:Estradiol; D004967:Estrogens / Q000378:metabolism*; D059630:Mesenchymal Stem Cells; D047495:PPAR gamma; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D004967:Estrogens; D047495:PPAR gamma; D004958:Estradiol