title: Metagenome Analysis Exploiting High-Throughput Chromosome Conformation Capture (3C) Data.
abstract: Microbial communities are complex and constitute important parts of our environment. Genomic analysis of these populations is a dynamic research area but remains limited by the difficulty in assembling full genomes of individual species. Recently, a new method for metagenome assembly/analysis based on chromosome conformation capture has emerged (meta3C). This approach quantifies the collisions experienced by DNA molecules to identify those sharing the same cellular compartments, allowing the characterization of genomes present within complex mixes of species. The exploitation of these chromosome 3D signatures holds promising perspectives for genome sequencing of discrete species in complex populations. It also has the potential to assign correctly extra-chromosomal elements, such as plasmids, mobile elements and phages, to their host cells.
authors.affiliation: Institut Pasteur, Department of Genomes and Genetics, Groupe Régulation Spatiale des Génomes, 75015 Paris, France; CNRS, UMR 3525, 75015 Paris, France.
authors.forename: Martial
authors.initials: M
authors.lastname: Marbouty
authors.affiliation: Institut Pasteur, Department of Genomes and Genetics, Groupe Régulation Spatiale des Génomes, 75015 Paris, France; CNRS, UMR 3525, 75015 Paris, France. Electronic address: romain.koszul@pasteur.fr.
authors.forename: Romain
authors.initials: R
authors.lastname: Koszul
mesh_terms: D002875:Chromosomes; D057166:High-Throughput Screening Assays; D054892:Metagenome