title: Enhancement of hypocotyl elongation by LOV KELCH PROTEIN2 production is mediated by auxin and phytochrome-interacting factors in Arabidopsis thaliana.
abstract: Auxin and two phytochrome-interacting factors, PHYTOCHROME-INTERACTING FACTOR4 (PIF4) and PIF5, play crucial roles in the enhancement of hypocotyl elongation in transgenic Arabidopsis thaliana plants that overproduce LOV KELCH PROTEIN2 (LKP2). LOV KELCH PROTEIN2 (LKP2) is a positive regulator of hypocotyl elongation under white light in Arabidopsis thaliana. In this study, using microarray analysis, we compared the gene expression profiles of hypocotyls of wild-type Arabidopsis (Columbia accession), a transgenic line that produces green fluorescent protein (GFP), and two lines that produce GFP-tagged LKP2 (GFP-LKP2). We found that, in GFP-LKP2 hypocotyls, 775 genes were up-regulated, including 36 auxin-responsive genes, such as 27 SMALL AUXIN UP RNA (SAUR) and 6 AUXIN/INDOLE-3-ACETIC ACID (AUX/IAA) genes, and 21 genes involved in responses to red or far-red light, including PHYTOCHROME-INTERACTING FACTOR4 (PIF4) and PIF5; and 725 genes were down-regulated, including 15 flavonoid biosynthesis genes. Hypocotyls of GFP-LKP2 seedlings, but not cotyledons or roots, contained a higher level of indole-3-acetic acid (IAA) than those of control seedlings. Auxin inhibitors reduced the enhancement of hypocotyl elongation in GFP-LKP2 seedlings by inhibiting the increase in cortical cell number and elongation of the epidermal and cortical cells. The enhancement of hypocotyl elongation was completely suppressed in progeny of the crosses between GFP-LKP2 lines and dominant gain-of-function auxin-resistant mutants (axr2-1 and axr3-1) or loss-of-function mutants pif4, pif5, and pif4 pif5. Our results suggest that the enhancement of hypocotyl elongation in GFP-LKP2 seedlings is due to the elevated level of IAA and to the up-regulated expression of PIF4 and PIF5 in hypocotyls.
authors.affiliation: Department of Life Science, Faculty of Science, Gakushuin University, 1-5-1 Mejiro, Toshima-Ku, Tokyo, 171-8588, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Miyazaki
authors.affiliation: Growth Regulation Research Group, RIKEN Plant Science Center, 1-7-22 Suehiro-cho, Tsurumi, Yokohama, Kanagawa, 230-0045, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Jikumaru
authors.affiliation: Department of Life Science, Faculty of Science, Gakushuin University, 1-5-1 Mejiro, Toshima-Ku, Tokyo, 171-8588, Japan.
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Takase
authors.affiliation: Department of Life Science, Faculty of Science, Gakushuin University, 1-5-1 Mejiro, Toshima-Ku, Tokyo, 171-8588, Japan.
authors.forename: Aya
authors.initials: A
authors.lastname: Saitoh
authors.affiliation: Department of Life Science, Faculty of Science, Gakushuin University, 1-5-1 Mejiro, Toshima-Ku, Tokyo, 171-8588, Japan.
authors.forename: Asuka
authors.initials: A
authors.lastname: Sugitani
authors.affiliation: Growth Regulation Research Group, RIKEN Plant Science Center, 1-7-22 Suehiro-cho, Tsurumi, Yokohama, Kanagawa, 230-0045, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Kamiya
authors.affiliation: Department of Life Science, Faculty of Science, Gakushuin University, 1-5-1 Mejiro, Toshima-Ku, Tokyo, 171-8588, Japan. tomohiro.kiyosue@gakushuin.ac.jp.
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Kiyosue
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D018546:Hypocotyl / Q000254:growth & development* / Q000378:metabolism*; D007210:Indoleacetic Acids / Q000378:metabolism*; D010834:Phytochrome / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D007210:Indoleacetic Acids; D010834:Phytochrome
keywords: Hypocotyl elongation; IAA; LKP2; Microarray; PIF; SAUR