title: Transcriptional regulation of PIN genes by FOUR LIPS and MYB88 during Arabidopsis root gravitropism.
abstract: PIN proteins are auxin export carriers that direct intercellular auxin flow and in turn regulate many aspects of plant growth and development including responses to environmental changes. The Arabidopsis R2R3-MYB transcription factor FOUR LIPS (FLP) and its paralogue MYB88 regulate terminal divisions during stomatal development, as well as female reproductive development and stress responses. Here we show that FLP and MYB88 act redundantly but differentially in regulating the transcription of PIN3 and PIN7 in gravity-sensing cells of primary and lateral roots. On the one hand, FLP is involved in responses to gravity stimulation in primary roots, whereas on the other, FLP and MYB88 function complementarily in establishing the gravitropic set-point angles of lateral roots. Our results support a model in which FLP and MYB88 expression specifically determines the temporal-spatial patterns of PIN3 and PIN7 transcription that are closely associated with their preferential functions during root responses to gravity.
authors.affiliation: Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, 20 Nanxincun, Xiangshan (Fragrant Hill), Haidian, Beijing 100093, China.|University of Chinese Academy of Sciences, 19A Yuquan Road, Beijing 100049, China.
authors.forename: Hong-Zhe
authors.initials: HZ
authors.lastname: Wang
authors.affiliation: Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, 20 Nanxincun, Xiangshan (Fragrant Hill), Haidian, Beijing 100093, China.
authors.forename: Ke-Zhen
authors.initials: KZ
authors.lastname: Yang
authors.affiliation: Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, 20 Nanxincun, Xiangshan (Fragrant Hill), Haidian, Beijing 100093, China.
authors.forename: Jun-Jie
authors.initials: JJ
authors.lastname: Zou
authors.affiliation: Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, 20 Nanxincun, Xiangshan (Fragrant Hill), Haidian, Beijing 100093, China.|University of Chinese Academy of Sciences, 19A Yuquan Road, Beijing 100049, China.
authors.forename: Ling-Ling
authors.initials: LL
authors.lastname: Zhu
authors.affiliation: Department of Molecular Genetics, Center for Applied Plant Sciences, The Ohio State University, Columbus, Ohio 43210, USA.
authors.forename: Zi Dian
authors.initials: ZD
authors.lastname: Xie
authors.affiliation: Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan.
authors.forename: Miyo Terao
authors.initials: MT
authors.lastname: Morita
authors.affiliation: Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma 630-0101, Japan.
authors.forename: Masao
authors.initials: M
authors.lastname: Tasaka
authors.affiliation: Institute of Science and Technology of Austria, Am Campus 1, Klosterneuburg 3400, Austria.
authors.forename: Jiří
authors.initials: J
authors.lastname: Friml
authors.affiliation: Department of Molecular Genetics, Center for Applied Plant Sciences, The Ohio State University, Columbus, Ohio 43210, USA.
authors.forename: Erich
authors.initials: E
authors.lastname: Grotewold
authors.affiliation: Department of Plant Systems Biology, VIB, Ghent B-9052, Belgium.|Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent B-9052, Belgium.
authors.forename: Tom
authors.initials: T
authors.lastname: Beeckman
authors.affiliation: Department of Plant Systems Biology, VIB, Ghent B-9052, Belgium.|Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent B-9052, Belgium.
authors.forename: Steffen
authors.identifier: http://orcid.org/0000-0002-9948-9672
authors.initials: S
authors.lastname: Vanneste
authors.affiliation: Department of Botany, University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z4.
authors.forename: Fred
authors.initials: F
authors.lastname: Sack
authors.affiliation: Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, 20 Nanxincun, Xiangshan (Fragrant Hill), Haidian, Beijing 100093, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Le
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000235:genetics*; D047369:Chromatin Immunoprecipitation; D024202:Electrophoretic Mobility Shift Assay; D018506:Gene Expression Regulation, Plant; D005966:Glucuronidase / Q000378:metabolism; D018522:Gravitropism / Q000235:genetics*; D017403:In Situ Hybridization; D030781:Organisms, Genetically Modified; D018517:Plant Roots / Q000254:growth & development* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D029681:Arabidopsis Proteins; C507998:FLP protein, Arabidopsis; C507999:Myb88 protein, Arabidopsis; C449300:PIN3 protein, Arabidopsis; C479554:PIN7 protein, Arabidopsis; D012333:RNA, Messenger; D014157:Transcription Factors; D005966:Glucuronidase