title: Genome-wide study on intramuscular fat in Italian Large White pig breed using the PorcineSNP60 BeadChip.
abstract: Genome-wide association study results are presented for intramuscular fat in Italian Large White pig breed. A total of 886 individuals were genotyped with PorcineSNP60 BeadChip. After quality control performed with plink software and in R environment, 49 208 markers remained for the association analysis. The genome-wide association studies was conducted using linear mixed model implemented in GenABEL. We detected seven new SNPs of genes till now not found associated to intramuscular fat (IMF). Three markers map in a wide intergenic region rich of QTL linked to fat traits, one map 388 kb upstream the gene SDK1, one map inside PPP3CA gene, one inside SCPEP1 gene and the last is not mapped in the porcine genome yet. Associations here presented indicate a moderate effect of these genes on IMF. In particular, PPP3CA, that is involved in the oxidative metabolism of skeletal muscle, could be considerated as an interesting candidate gene for IMF content in pigs. However, further studies are needed to clarify the role of these genes on the physiological processes involved in IMF regulation. These results may be useful to control this trait that is important in terms of nutritional, technological and organoleptic characteristics of fresh meat and processed products.
authors.affiliation: Department of Agriculture and Food Sciences DISTAL, Bologna University, Bologna, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Davoli
authors.affiliation: Department of Agriculture and Food Sciences DISTAL, Bologna University, Bologna, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Luise
authors.affiliation: Department of Agriculture and Food Sciences DISTAL, Bologna University, Bologna, Italy.
authors.forename: V
authors.initials: V
authors.lastname: Mingazzini
authors.affiliation: Department of Agriculture and Food Sciences DISTAL, Bologna University, Bologna, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Zambonelli
authors.affiliation: Department of Agriculture and Food Sciences DISTAL, Bologna University, Bologna, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Braglia
authors.affiliation: Dipartimento di Scienze Agrarie, Alimentari e Agro-ambientali, Pisa University, Pisa, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Serra
authors.affiliation: Department of Agriculture and Food Sciences DISTAL, Bologna University, Bologna, Italy.
authors.forename: V
authors.initials: V
authors.lastname: Russo
mesh_terms: D000273:Adipose Tissue / Q000737:chemistry; D000818:Animals; D055106:Genome-Wide Association Study; D000070633:Hamstring Muscles / Q000737:chemistry*; D020641:Polymorphism, Single Nucleotide; D040641:Quantitative Trait Loci; D034421:Sus scrofa / Q000097:blood / Q000145:classification / Q000235:genetics* / Q000254:growth & development
keywords: Candidate genes; Intramuscular fat deposition; meat quality traits; porcine genome