title: In vitro fermentation of juçara pulp (Euterpe edulis) by human colonic microbiota.
abstract: This study was carried out to investigate the potential fermentation properties of juçara pulp, using pH-controlled anaerobic batch cultures reflective of the distal region of the human large intestine. Effects upon major groups of the microbiota were monitored over 24h incubations by fluorescence in situ hybridisation (FISH). Short-chain fatty acids (SCFA) were measured by HPLC. Phenolic compounds, during an in vitro simulated digestion and fermentation, were also analysed. Juçara pulp can modulate the intestinal microbiota in vitro, promoting changes in the relevant microbial populations and shifts in the production of SCFA. Fermentation of juçara pulp resulted in a significant increase in numbers of bifidobacteria after a 24h fermentation compared to a negative control. After in vitro digestion, 46% of total phenolic content still remained. This is the first study reporting the potential prebiotic effect of juçara pulp; however, human studies are necessary to prove its efficacy.
authors.affiliation: Department of Food and Nutrition Sciences, The University of Reading, RG6 6AP Reading, UK; Department of Food Science and Technology, State University of Londrina, Celso Garcia Cid Road, Km 380, C.P.:6001, 86051-970 Londrina, Brazil. Electronic address: karla2901@gmail.com.
authors.forename: Karla Bigetti
authors.initials: KB
authors.lastname: Guergoletto
authors.affiliation: Department of Food and Nutrition Sciences, The University of Reading, RG6 6AP Reading, UK; Health Sciences Research Centre, Life Sciences Department, Whitelands College, University of Roehampton, London, UK.
authors.forename: Adele
authors.initials: A
authors.lastname: Costabile
authors.affiliation: Consejo Superior de Investigaciones Científicas - CSIC, Madrid, Spain.
authors.forename: Gema
authors.initials: G
authors.lastname: Flores
authors.affiliation: Department of Food Science and Technology, State University of Londrina, Celso Garcia Cid Road, Km 380, C.P.:6001, 86051-970 Londrina, Brazil.
authors.forename: Sandra
authors.initials: S
authors.lastname: Garcia
authors.affiliation: Department of Food and Nutrition Sciences, The University of Reading, RG6 6AP Reading, UK.
authors.forename: Glenn R
authors.initials: GR
authors.lastname: Gibson
mesh_terms: D065748:Euterpe / Q000737:chemistry*; D005243:Feces / Q000382:microbiology*; D005285:Fermentation; D005638:Fruit / Q000737:chemistry*; D000069196:Gastrointestinal Microbiome / Q000502:physiology*; D006801:Humans; D066298:In Vitro Techniques
keywords: Batch culture fermentation system; Faecal microbiota; Juçara; Phenolic compounds