title: Biological Evaluation (In Vitro and In Vivo) of Bilayered Collagenous Coated (Nano Electrospun and Solid Wall) Chitosan Membrane for Periodontal Guided Bone Regeneration.
abstract: The application of barrier membranes in guided bone regeneration (GBR) has become a commonly used surgical technique in periodontal research. The objectives of this study were to evaluate the in vitro biocompatibility and osteogenic differentiation of mesenchymal stem cells (MSCs) on two different collagenous coatings (nano electrospun fibrous vs. solid wall) of bilayered collagen/chitosan membrane and their histological evaluation on bone regeneration in rabbit calvarial defects. It was found that chitosan-nano electrospun collagen (CNC) membranes had higher proliferation/metabolic activity compared to the chitosan-collagen (CC) and pristine chitosan membranes. The qRT-PCR analysis demonstrated the CNC membranes induced significant expression of osteogenic genes (Osteocalcin, RUNX2 and Col-α1) in MSCs. Moreover, higher calcium content and alkaline phosphatase activity of MSCs were observed compared to the other groups. Histologic and histomorphometric evaluations were performed on the uncovered (negative control) as well as covered calvarial defects of ten adult white rabbits with different membranes (CNC, CC, BioGide (BG, positive control)) at 1 and 2 months after surgery. More bone formation was detected in the defects covered with CNC and BG membranes than those covered by CC and the negative control. No inflammation and residual biomaterial particles were observed on the membrane surface or in the surrounding tissues in the surgical areas. These results suggest that bilayer CNC membrane can have the potential for use as a GBR membrane material facilitating bone formation.
authors.affiliation: Dental Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.|Department of Periodontology, Dental School, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Ghogha
authors.initials: G
authors.lastname: Lotfi
authors.affiliation: National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Mohammad Ali
authors.initials: MA
authors.lastname: Shokrgozar
authors.affiliation: Dental Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.|Department of Periodontology, Dental School, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Rasoul
authors.initials: R
authors.lastname: Mofid
authors.affiliation: Department of Pathology of Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Fatemeh Mashhadi
authors.initials: FM
authors.lastname: Abbas
authors.affiliation: Dental Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Farzin
authors.initials: F
authors.lastname: Ghanavati
authors.affiliation: Department of Basic Sciences, Proteomics Research Center, School of Rehabilitation Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Alireza Akbarzadeh
authors.initials: AA
authors.lastname: Baghban
authors.affiliation: National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Seyedeh Kimia
authors.initials: SK
authors.lastname: Yavari
authors.affiliation: Department of Chemical Engineering, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, G. C., Evin, 1983963113, Tehran, Iran. pajoumsh@cec.sc.edu.|Department of Chemical Engineering, University of South Carolina, Columbia, SC, USA. pajoumsh@cec.sc.edu.
authors.forename: Seyedramin
authors.initials: S
authors.lastname: Pajoumshariati
mesh_terms: D000818:Animals; D000943:Antigens, Differentiation / Q000096:biosynthesis; D001861:Bone Regeneration; D048271:Chitosan / Q000737:chemistry / Q000494:pharmacology; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000494:pharmacology; D008297:Male; D008422:Materials Testing; D008567:Membranes, Artificial; D010519:Periodontium / Q000293:injuries*; D011817:Rabbits
chemical_list: D000943:Antigens, Differentiation; D020099:Coated Materials, Biocompatible; D008567:Membranes, Artificial; D048271:Chitosan
keywords: Chitosan; Collagen; Electrospun nanofiber; Guided bone regeneration membrane; MSC differentiation; Solid wall