title: Characterization of siRNAs derived from cucumber green mottle mosaic virus in infected cucumber plants.
abstract: Virus-derived small interfering RNAs (vsiRNAs) of cucumber green mottle mosaic virus (CGMMV), a member of the genus Tobamovirus, were characterised in cucumber plants by deep sequencing. CGMMV vsiRNAs of 21-22 nt in length predominated, suggesting that there might be a conserved mechanism of DCL2 and DCL4 involvement in the biogenesis of vsiRNAs, as well as a common RNA silencing pathway in CGMMV-infected cucumber plants. The 5'-terminal base of vsiRNAs was biased towards C/A/U, suggesting that CGMMV vsiRNAs might be loaded into diverse AGO-containing RISCs to disturb the gene expression of host plants. Possible targets for some of the vsiRNAs were also predicted.
authors.affiliation: College of Plant Science and Technology, Beijing University of Agriculture, Beijing, 102206, China. lyq047@163.com.
authors.forename: Yongqiang
authors.initials: Y
authors.lastname: Li
authors.affiliation: Plant Laboratory of Beijing Entry-Exit Inspection and Quarantine Bureau, Beijing, 101312, China.
authors.forename: Congliang
authors.initials: C
authors.lastname: Deng
authors.affiliation: College of Plant Science and Technology, Beijing University of Agriculture, Beijing, 102206, China.
authors.forename: Qiaoxia
authors.initials: Q
authors.lastname: Shang
authors.affiliation: Plant Laboratory of Beijing Entry-Exit Inspection and Quarantine Bureau, Beijing, 101312, China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Plant Laboratory of Beijing Entry-Exit Inspection and Quarantine Bureau, Beijing, 101312, China.
authors.forename: Xingliang
authors.initials: X
authors.lastname: Liu
authors.affiliation: Chinese Society of Inspection and Quarantine, Beijing, 100029, China. zhouq@bjciq.gov.cn.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhou
mesh_terms: D018553:Cucumis sativus / Q000821:virology*; D005786:Gene Expression Regulation; D059014:High-Throughput Nucleotide Sequencing; D054884:Host-Pathogen Interactions; D010935:Plant Diseases / Q000821:virology*; D034741:RNA, Small Interfering / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*; D017898:Tobamovirus / Q000235:genetics*
chemical_list: D034741:RNA, Small Interfering; D012367:RNA, Viral