title: New findings about trichodysplasia spinulosa-associated polyomavirus (TSPyV)--novel qPCR detects TSPyV-DNA in blood samples.
abstract: A new real-time PCR assay for trichodysplasia spinulosa-associated polyomavirus (TSPyV) DNA detection was designed, and blood samples from kidney transplant recipients and healthy individuals were screened. TSPyV-DNA was not detected in blood from healthy individuals, but 26.8% of kidney recipients presented TSPyV-DNA. This is the first report of TSPyV viremia.
authors.affiliation: São Paulo Institute of Tropical Medicine, São Paulo, Brazil.
authors.forename: Paulo R
authors.initials: PR
authors.lastname: Urbano
authors.affiliation: São Paulo Institute of Tropical Medicine, São Paulo, Brazil.
authors.forename: Luiz H S
authors.initials: LH
authors.lastname: Nali
authors.affiliation: University of São Paulo, São Paulo, Brazil.
authors.forename: Camila S
authors.initials: CS
authors.lastname: Bicalho
authors.affiliation: University of São Paulo, São Paulo, Brazil.
authors.forename: Ligia C
authors.initials: LC
authors.lastname: Pierrotti
authors.affiliation: University of São Paulo, São Paulo, Brazil.
authors.forename: Elias
authors.initials: E
authors.lastname: David-Neto
authors.affiliation: São Paulo Institute of Tropical Medicine, São Paulo, Brazil.
authors.forename: Cláudio S
authors.initials: CS
authors.lastname: Pannuti
authors.affiliation: São Paulo Institute of Tropical Medicine, São Paulo, Brazil. Electronic address: cmromano@usp.br.
authors.forename: Camila M
authors.initials: CM
authors.lastname: Romano
mesh_terms: D000328:Adult; D000368:Aged; D001769:Blood / Q000821:virology*; D004279:DNA, Viral / Q000097:blood*; D006201:Hair Diseases / Q000821:virology*; D006801:Humans; D016030:Kidney Transplantation / Q000009:adverse effects; D008875:Middle Aged; D027382:Polyomaviridae / Q000235:genetics / Q000302:isolation & purification*; D027601:Polyomavirus Infections / Q000821:virology*; D060888:Real-Time Polymerase Chain Reaction; D066027:Transplant Recipients; D014766:Viremia / Q000175:diagnosis
chemical_list: D004279:DNA, Viral
keywords: Blood; Immunocompromised patient; Polyomavirus; Real-time PCR; TSPyV