title: Effects of berberine and pomegranate seed oil on plasma phospholipid metabolites associated with risks of type 2 diabetes mellitus by U-HPLC/Q-TOF-MS.
abstract: A rapid and reliable ultra-performance liquid chromatography coupled with electrospray ionization/quadrupole-time-of-flight mass spectrometry (U-HPLC/Q-TOF-MS) has been firstly used to analyze the changes of plasma phospholipids, in type 2 diabetes mellitus (T2DM) mice after administration of berberine and pomegranate seed oil (PSO). The separation of plasma phospholipids was carried out on an Acquity U-HPLC BEH C18 column (2.1mm×50mm, 1.7μm, Waters) by linear gradient elution using a mobile phase consisting of 10mM ammonium formate in water and acetonitrile: isopropanol (1:1, v/v) mixed solution added by 0.25% water and 10mM ammonium formate. The method demonstrated a good precision and reproducibility. Linear regression analysis showed a good linearity. And potential biomarkers were discovered based on their mass spectra and chemometrics methods. The results demonstrated that the proposed U-HPLC/Q-TOF-MS method was successfully applied to analyze the dynamic changes of phospholipids components in plasma of T2DM mice after drug treatment and could provide a useful data base for meriting further study in humans and investigating pharmacological actions of drugs.
authors.affiliation: Center Laboratory, Guangdong Pharmaceutical University, Guangzhou 510006, People's Republic of China.
authors.forename: Xia
authors.initials: X
authors.lastname: Wu
authors.affiliation: School of Chinese Material Medical, Guangzhou University of Chinese Medicine, Guangzhou 510006, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Center Laboratory, Guangdong Pharmaceutical University, Guangzhou 510006, People's Republic of China.
authors.forename: Qiu
authors.initials: Q
authors.lastname: Wang
authors.affiliation: School of Chinese Material Medical, Guangzhou University of Chinese Medicine, Guangzhou 510006, People's Republic of China. Electronic address: 13925023915@139.com.
authors.forename: Weimin
authors.initials: W
authors.lastname: Li
authors.affiliation: Center Laboratory, Guangdong Pharmaceutical University, Guangzhou 510006, People's Republic of China. Electronic address: yffeng@139.com.
authors.forename: Yifan
authors.initials: Y
authors.lastname: Feng
mesh_terms: D000818:Animals; D001599:Berberine / Q000494:pharmacology*; D015415:Biomarkers / Q000097:blood; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003924:Diabetes Mellitus, Type 2 / Q000097:blood*; D029561:Lythraceae / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods*; D055432:Metabolomics; D051379:Mice; D010743:Phospholipids / Q000097:blood*; D010938:Plant Oils / Q000494:pharmacology*; D015203:Reproducibility of Results; D012307:Risk Factors; D012639:Seeds / Q000737:chemistry*
chemical_list: D015415:Biomarkers; D010743:Phospholipids; D010938:Plant Oils; D001599:Berberine
keywords: Berberine; Biomarkers; Plasma phospholipids; Pomegranate seed oil; U-HPLC/Q-TOF-MS