title: Chemical Profiles and Protective Effect of Hedyotis diffusa Willd in Lipopolysaccharide-Induced Renal Inflammation Mice.
abstract: Protective effect of Hedyotis diffusa (H. diffusa) Willd against lipopolysaccharide (LPS)-induced renal inflammation was evaluated by the productions of cytokines and chemokine, and the bioactive constituents of H. diffusa were detected by the ultra-fast liquid chromatography-diode array detector-quadrupole-time of flight mass spectrometry (UFLC-DAD-Q-TOF-MS/MS) method. As the results showed, water extract of H. diffusa (equal to 5.0 g/kg body weight) obviously protected renal tissues, significantly suppressed the productions of tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, IL-6, and monocyte chemoattractant protein (MCP)-1, as well as significantly promoted the production of IL-10 in serum and renal tissues. According the chemical profiles of H. diffusa, flavonoids, iridoid glycosides and anthraquinones were greatly detected in serum from H. diffusa extract treatment mice. Two main chemotypes, including eight flavonoids and four iridoid glycosides were found in renal tissues from H. diffusa extract treatment mice. The results demonstrated that water extract of H. diffusa had protective effect on renal inflammation, which possibly resulted from the bioactive constituents consisting of flavonoids, iridoids and anthraquinones.
authors.affiliation: Department of Endocrinology, Foshan Hospital of Traditional Chinese Medicine, Foshan 528000, China. yejianhong2005@163.com.
authors.forename: Jian-Hong
authors.initials: JH
authors.lastname: Ye
authors.affiliation: Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, China. menghua_liu@hotmail.com.|State Key Laboratory of Organ Failure Research, Guangdong Provincial Institute of Nephrology, Southern Medical University, Guangzhou 510515, China. menghua_liu@hotmail.com.
authors.forename: Meng-Hua
authors.initials: MH
authors.lastname: Liu
authors.affiliation: Bioengineering Research Center, Guangzhou Institute of Advanced Technology, Chinese Academy of Sciences, Guangzhou 511458, China. zxl_lxz@live.com.
authors.forename: Xu-Lin
authors.initials: XL
authors.lastname: Zhang
authors.affiliation: Bioengineering Research Center, Guangzhou Institute of Advanced Technology, Chinese Academy of Sciences, Guangzhou 511458, China. jy.he@giat.ac.cn.
authors.forename: Jing-Yu
authors.initials: JY
authors.lastname: He
mesh_terms: D000818:Animals; D018925:Chemokines / Q000096:biosynthesis; D002851:Chromatography, High Pressure Liquid; D016207:Cytokines / Q000096:biosynthesis; D032064:Hedyotis / Q000737:chemistry*; D008070:Lipopolysaccharides / Q000009:adverse effects; D008264:Macrophages / Q000378:metabolism / Q000473:pathology; D051379:Mice; D009393:Nephritis / Q000139:chemically induced / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D020011:Protective Agents / Q000737:chemistry / Q000494:pharmacology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry
chemical_list: D018925:Chemokines; D016207:Cytokines; D008070:Lipopolysaccharides; D010936:Plant Extracts; D020011:Protective Agents
keywords: Hedyotis diffusa Willd; anthraquinones; chemical profiles; flavonoids; iridoids; renal inflammation