title: Study on size effect of the silica nanospheres with solid core and mesoporous shell on cellular uptake.
abstract: The properties of mesoporous silica nanoparticles including large surface area, large pore volume, easy surface functionalization and control of structure and pore size has made them promising drug carriers. In this study, the effect of different diameters (50 nm, 70 nm, 90 nm, 110 nm and 140 nm) of silica nanospheres with a solid core and mesoporous shell (mSiO2/SiO2) on cellular internalization in mouse fibroblast cells (L929) was evaluated. The physical properties of the nanostructures were characterized with various methods, such as transmission electron microscopy with x-ray dispersion spectroscopy, thermogravimetric analysis, Fourier transform infrared spectroscopy and zeta potential. In order to define the cellular uptake, the nanostructures were labelled with fluorescent dye Alexa647, and imaging and quantitative methods were applied: laser scanning confocal microscopy, flow cytometry and thermogravimetry. Our results indicate that cellular uptake of the studied nanospheres is size-dependent, and nanospheres of 90 nm in diameter showed the most efficient cell internalization. Thus, particle size is an important parameter that determines cellular uptake of nanoparticles and should be considered in designing drug delivery carriers.
authors.affiliation: Department of Experimental & Clinical Pharmacology, Pomeranian Medical University, Powstancow Wlkp. 72, 70-111 Szczecin, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Peruzynska
authors.forename: K
authors.initials: K
authors.lastname: Cendrowski
authors.forename: M
authors.initials: M
authors.lastname: Barylak
authors.forename: D
authors.initials: D
authors.lastname: Roginska
authors.forename: M
authors.initials: M
authors.lastname: Tarnowski
authors.forename: M
authors.initials: M
authors.lastname: Tkacz
authors.forename: M
authors.initials: M
authors.lastname: Kurzawski
authors.forename: B
authors.initials: B
authors.lastname: Machalinski
authors.forename: E
authors.initials: E
authors.lastname: Mijowska
authors.forename: M
authors.initials: M
authors.lastname: Drozdzik
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis*; D002460:Cell Line; D004058:Diffusion; D005347:Fibroblasts / Q000737:chemistry*; D008422:Materials Testing; D051379:Mice; D058608:Nanopores / Q000648:ultrastructure*; D054118:Nanospheres / Q000737:chemistry* / Q000648:ultrastructure*; D010316:Particle Size; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D012822:Silicon Dioxide