title: Three Peptides from Soy Glycinin Modulate Glucose Metabolism in Human Hepatic HepG2 Cells.
abstract: Ile-Ala-Val-Pro-Gly-Glu-Val-Ala (IAVPGEVA), Ile-Ala-Val-Pro-Thr-Gly-Val-Ala (IAVPTGVA) and Leu-Pro-Tyr-Pro (LPYP), three peptides deriving from soy glycinin hydrolysis, are known to regulate cholesterol metabolism in human hepatic HepG2 cells. We have recently demonstrated that the mechanism of action involves the activation of adenosine monophosphate-activated protein kinase (AMPK). This fact suggested a potential activity of the same peptides on glucose metabolism that prompted us to also investigate this aspect in the same cells. After treatment with IAVPGEVA, IAVPTGVA and LPYP, HepG2 cells were analyzed using a combination of molecular techniques, including western blot analysis, glucose uptake experiments and fluorescence microscopy evaluation. The results showed that these peptides are indeed able to enhance the capacity of HepG2 cells to uptake glucose, via glucose transporter 1 GLUT1 and glucose transporter 4 GLUT4 activation, through the stimulation of protein kinase B Akt and adenosine monophosphate-activated protein kinase AMPK pathways, both involved in glucose metabolism.
authors.affiliation: Department of Pharmaceutical Sciences, University of Milan, Mangiagalli Street 25, 20133 Milan, Italy. carmen.lammi@unimi.it.
authors.forename: Carmen
authors.initials: C
authors.lastname: Lammi
authors.affiliation: Department of Pharmaceutical Sciences, University of Milan, Mangiagalli Street 25, 20133 Milan, Italy. chiara.zanoni1@unimi.it.
authors.forename: Chiara
authors.initials: C
authors.lastname: Zanoni
authors.affiliation: Department of Pharmaceutical Sciences, University of Milan, Mangiagalli Street 25, 20133 Milan, Italy. anna.arnoldi@unimi.it.
authors.forename: Anna
authors.initials: A
authors.lastname: Arnoldi
mesh_terms: D050260:Carbohydrate Metabolism / Q000187:drug effects*; D005916:Globulins / Q000737:chemistry*; D005947:Glucose / Q000378:metabolism*; D051246:Glucose Transport Proteins, Facilitative / Q000378:metabolism; D038362:Glycogen Synthase Kinase 3 / Q000378:metabolism; D056945:Hep G2 Cells; D006801:Humans; D008954:Models, Biological; D010446:Peptide Fragments / Q000737:chemistry / Q000494:pharmacology*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D030262:Soybean Proteins / Q000737:chemistry*
chemical_list: D005916:Globulins; D051246:Glucose Transport Proteins, Facilitative; D010446:Peptide Fragments; D030262:Soybean Proteins; C011474:glycinin; D051057:Proto-Oncogene Proteins c-akt; D038362:Glycogen Synthase Kinase 3; D005947:Glucose
keywords: HepG2 cell line; bioactive peptides; functional foods; glucose uptake; plant proteins