title: Evaluation of the Antimicrobial Effect of Chitosan/Polyvinyl Alcohol Electrospun Nanofibers Containing Mafenide Acetate.
abstract: BACKGROUND
  Chitosan, an important biodegradable and biocompatible polymer, has demonstrated wound-healing and antimicrobial properties.
  
  
  OBJECTIVE
  This study aimed to evaluate the antimicrobial properties of mafenide acetate-loaded nanofibrous films, prepared by the electrospinning technique, using chitosan and polyvinyl alcohol (PVA).
  
  
  METHODS
  A 3(2) full factorial design was used for formulating electrospinning solutions. The chitosan percentage in chitosan/PVA solutions (0%, 10%, and 30%) and the drug content (0%, 20%, and 40%) were chosen as independent variables. The release rate of mafenide acetate from nanofibrous films and their microbial penetration were evaluated. The antimicrobial activity of different nanofibrous film formulations against Staphylococcus aureus and Pseudomonas aeruginosa was studied.
  
  
  RESULTS
  The results indicated that all nanofibrous films, with and without drug, can prevent bacterial penetration. Incorporation of mafenide acetate into chitosan/PVA nanofibers enhanced their antimicrobial activity against P. aeruginosa and S. aureus.
  
  
  CONCLUSIONS
  Overall, the results showed that chitosan/polyvinyl alcohol (PVA) nanofibrous films are applicable for use as a wound dressing with protective, healing, and antimicrobial effects.
authors.affiliation: Nanotechnology Research Center, School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran.
authors.forename: Mohammadreza
authors.initials: M
authors.lastname: Abbaspour
authors.affiliation: Nanotechnology Research Center, School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran.
authors.forename: Behzad
authors.initials: B
authors.lastname: Sharif Makhmalzadeh
authors.affiliation: Nanotechnology Research Center, School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran.
authors.forename: Behjat
authors.initials: B
authors.lastname: Rezaee
authors.affiliation: Department of Microbiology, Infectious and Tropical Diseases Research Center, Faculty of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, IR Iran.
authors.forename: Saeed
authors.initials: S
authors.lastname: Shoja
authors.affiliation: Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University, Ahvaz, IR Iran.
authors.forename: Zohreh
authors.initials: Z
authors.lastname: Ahangari
keywords: Burns; Chitosan; Mafenide; Nanofibers; Polyvinyl Alcohol