title: Chelator-Free Labeling of Layered Double Hydroxide Nanoparticles for in Vivo PET Imaging.
abstract: Layered double hydroxide (LDH) nanomaterial has emerged as a novel delivery agent for biomedical applications due to its unique structure and properties. However, in vivo positron emission tomography (PET) imaging with LDH nanoparticles has not been achieved. The aim of this study is to explore chelator-free labeling of LDH nanoparticles with radioisotopes for in vivo PET imaging. Bivalent cation (64)Cu(2+) and trivalent cation (44)Sc(3+) were found to readily label LDH nanoparticles with excellent labeling efficiency and stability, whereas tetravalent cation (89)Zr(4+) could not label LDH since it does not fit into the LDH crystal structure. PET imaging shows that prominent tumor uptake was achieved in 4T1 breast cancer with (64)Cu-LDH-BSA via passive targeting alone (7.7 ± 0.1%ID/g at 16 h post-injection; n = 3). These results support that LDH is a versatile platform that can be labeled with various bivalent and trivalent radiometals without comprising the native properties, highly desirable for PET image-guided drug delivery.
authors.affiliation: Materials Science Program, University of Wisconsin-Madison, WI, USA.
authors.forename: Sixiang
authors.initials: S
authors.lastname: Shi
authors.affiliation: Department of Radiology, University of Wisconsin-Madison, WI, USA.
authors.forename: Brianne C
authors.initials: BC
authors.lastname: Fliss
authors.affiliation: Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, QLD, Australia.
authors.forename: Zi
authors.initials: Z
authors.lastname: Gu
authors.affiliation: Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, QLD, Australia.
authors.forename: Yian
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Department of Radiology, University of Wisconsin-Madison, WI, USA.
authors.forename: Hao
authors.initials: H
authors.lastname: Hong
authors.affiliation: Department of Medical Physics, University of Wisconsin-Madison, WI, USA.
authors.forename: Hector F
authors.initials: HF
authors.lastname: Valdovinos
authors.affiliation: Department of Medical Physics, University of Wisconsin-Madison, WI, USA.
authors.forename: Reinier
authors.initials: R
authors.lastname: Hernandez
authors.affiliation: Materials Science Program, University of Wisconsin-Madison, WI, USA.
authors.forename: Shreya
authors.initials: S
authors.lastname: Goel
authors.affiliation: Department of Radiology, University of Wisconsin-Madison, WI, USA.
authors.forename: Haiming
authors.initials: H
authors.lastname: Luo
authors.affiliation: Department of Radiology, University of Wisconsin-Madison, WI, USA.
authors.forename: Feng
authors.initials: F
authors.lastname: Chen
authors.affiliation: Department of Medical Physics, University of Wisconsin-Madison, WI, USA.
authors.forename: Todd E
authors.initials: TE
authors.lastname: Barnhart
authors.affiliation: Department of Medical Physics, University of Wisconsin-Madison, WI, USA.
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Nickles
authors.affiliation: Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, QLD, Australia.
authors.forename: Zhi Ping
authors.initials: ZP
authors.lastname: Xu
authors.affiliation: Materials Science Program, University of Wisconsin-Madison, WI, USA.|Department of Radiology, University of Wisconsin-Madison, WI, USA.|Department of Medical Physics, University of Wisconsin-Madison, WI, USA.|University of Wisconsin Carbone Cancer Center, Madison, WI, USA.
authors.forename: Weibo
authors.initials: W
authors.lastname: Cai
mesh_terms: D000818:Animals; D002417:Cattle; D045744:Cell Line, Tumor; D002614:Chelating Agents / Q000737:chemistry; D003301:Copper Radioisotopes / Q000737:chemistry; D005260:Female; D006878:Hydroxides / Q000737:chemistry* / Q000493:pharmacokinetics; D007553:Isotope Labeling / Q000379:methods*; D008325:Mammary Neoplasms, Experimental / Q000000981:diagnostic imaging* / Q000378:metabolism / Q000473:pathology; D008807:Mice, Inbred BALB C; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D049268:Positron-Emission Tomography / Q000379:methods*; D011868:Radioisotopes / Q000737:chemistry; D012538:Scandium / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000493:pharmacokinetics; D014018:Tissue Distribution; D015040:Zirconium / Q000737:chemistry
chemical_list: D002614:Chelating Agents; D003301:Copper Radioisotopes; D006878:Hydroxides; D011868:Radioisotopes; D012710:Serum Albumin, Bovine; D015040:Zirconium; D012538:Scandium