title: Vaginal Protection by H2O2-Producing Lactobacilli.
abstract: BACKGROUND
  Peroxide-producing lactobacilli provide protection from infection for the female reproductive tract. However, in vitro studies demonstrated that H2O2-produced by Lactobacillus is not the cause of inhibition of pathogens. It is not exactly known how H2O2-producing lactobacilli are involved in the protection of the vaginal environment.
  
  
  OBJECTIVE
  This study aimed to evaluate the importance of the interaction between H2O2-producing lactobacilli and their host for the resistance of the vaginal biotope.
  
  
  METHODS
  In this study, we used vaginal lactobacilli (11 H2O2-roducing strains and 11 non-H2O2-producing strains). The influence of epithelial cells on the growth and antibacterial activity of lactobacilli were evaluated. The effects of lactobacilli on the antibacterial activity of the epithelial cells, muramidase and lactoferrin were also determined.
  
  
  RESULTS
  Vaginal epithelial cells stimulated the growth and antibacterial activity of H2O2-producing lactobacilli in a greater extent than that of the non-H2O2-producing lactobacilli. Mainly, the H2O2-producing lactobacilli were capable of increasing the activity of the host antimicrobial peptides (muramidase and lactoferrin) as well as the antibacterial activity of the epithelial cells.
  
  
  CONCLUSIONS
  The involvement of the peroxide-producing lactobacilli in the protection of vagina was due to their ability to effectively interact with the host. This is expressed on one side to stimulate the growth and antagonistic activity of lactobacilli and on the other side to increase the antibacterial activity of the host defense factors (muramidase, lactoferrin and metabolites of epithelial cells).
authors.affiliation: Institute of Cellular and Intracellular Symbiosis, Orenburg, Russia.
authors.forename: Andrey
authors.initials: A
authors.lastname: V Sgibnev
authors.affiliation: Institute of Cellular and Intracellular Symbiosis, Orenburg, Russia.
authors.forename: Elena
authors.initials: E
authors.lastname: A Kremleva
keywords: Epithelial Cells; Host-Pathogen Interactions; Hydrogen Peroxide; Immunity; Innate; Lactobacillus; Lactoferrin; Muramidase; Vagina