title: Chikungunya Virus Replication in Salivary Glands of the Mosquito Aedes albopictus.
abstract: Chikungunya virus (CHIKV) is an emerging arbovirus transmitted to humans by mosquitoes such as Aedes albopictus. To be transmitted, CHIKV must replicate in the mosquito midgut, then disseminate in the hemocele and infect the salivary glands before being released in saliva. We have developed a standardized protocol to visualize viral particles in the mosquito salivary glands using transmission electron microscopy. Here we provide direct evidence for CHIKV replication and storage in Ae. albopictus salivary glands.
authors.affiliation: Arboviruses and Insect Vectors Unit, Department of Virology, Institute Pasteur, 25-28 rue du Docteur Roux, 75724 Paris cedex 15, France. anubis.vega-rua@pasteur.fr.
authors.forename: Anubis
authors.initials: A
authors.lastname: Vega-Rúa
authors.affiliation: Ultrapole, Center for Innovation and Technology Research, Institute Pasteur, 25-28 rue du Docteur Roux, 75724 Paris cedex 15, France. christine.schmitt@pasteur.fr.
authors.forename: Christine
authors.initials: C
authors.lastname: Schmitt
authors.affiliation: Ultrapole, Center for Innovation and Technology Research, Institute Pasteur, 25-28 rue du Docteur Roux, 75724 Paris cedex 15, France. isabelle.bonne@pasteur.fr.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Bonne
authors.affiliation: Ultrapole, Center for Innovation and Technology Research, Institute Pasteur, 25-28 rue du Docteur Roux, 75724 Paris cedex 15, France. jacomina.krijnse-locker@pasteur.fr.
authors.forename: Jacomine
authors.initials: J
authors.lastname: Krijnse Locker
authors.affiliation: Arboviruses and Insect Vectors Unit, Department of Virology, Institute Pasteur, 25-28 rue du Docteur Roux, 75724 Paris cedex 15, France. anna-bella.failloux@pasteur.fr.
authors.forename: Anna-Bella
authors.initials: AB
authors.lastname: Failloux
mesh_terms: D000330:Aedes / Q000821:virology*; D000818:Animals; D002646:Chikungunya virus / Q000254:growth & development* / Q000502:physiology; D005260:Female; D046529:Microscopy, Electron, Transmission; D012469:Salivary Glands / Q000821:virology; D014771:Virion / Q000648:ultrastructure; D014779:Virus Replication
keywords: Aedes albopictus; Chikungunya virus; budding; replication; salivary glands; transmission electron microscopy