title: C- and O-glycosyl flavonoids in Sanguinello and Tarocco blood orange (Citrus sinensis (L.) Osbeck) juice: Identification and influence on antioxidant properties and acetylcholinesterase activity.
abstract: Sanguinello and Tarocco are the blood orange (Citrus sinensis (L.) Osbeck) cultivars most diffused worldwide. Reversed phase liquid chromatography coupled with MS-MS analysis showed that these two varieties have a similar chromatographic pattern, characterised by the presence of C- and O-glycosyl flavonoids. Of the two, Sanguinello was found to be far richer in flavonoids than Tarocco. In the juices, twelve individual components were identified for the first time, namely, four C-glycosyl flavones (lucenin-2, vicenin-2, stellarin-2, lucenin-2 4'-methyl ether and scoparin), three flavonol derivatives (quercetin-3-O-(2-rhamnosyl)-rutinoside, quercetin-3-O-hexoside, quercetin 3-hydroxy-3-methylglutaryl-glycoside), an O-triglycosyl flavanone (narirutin 4'-O-glucoside) and a flavone O-glycosides (chrysoeriol 7-O-neoesperidoside). Moreover, the influence of the identified C- and O-glycosyl flavonoids on the antioxidant and acetylcholinesterase activity of these juices has been evaluated.
authors.affiliation: Dipartimento di Scienze Chimiche, Università di Messina, Viale F. Stagno d'Alcontres 31, 98166 Messina, Italy.
authors.forename: Davide
authors.initials: D
authors.lastname: Barreca
authors.affiliation: Dipartimento di Scienze Chimiche, Università di Messina, Viale F. Stagno d'Alcontres 31, 98166 Messina, Italy. Electronic address: ggattuso@unime.it.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Gattuso
authors.affiliation: Dipartimento di Scienze Chimiche, Università di Messina, Viale F. Stagno d'Alcontres 31, 98166 Messina, Italy.
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Laganà
authors.affiliation: Dipartimento di Scienze Chimiche, Università di Messina, Viale F. Stagno d'Alcontres 31, 98166 Messina, Italy.
authors.forename: Ugo
authors.initials: U
authors.lastname: Leuzzi
authors.affiliation: Dipartimento di Scienze Chimiche, Università di Messina, Viale F. Stagno d'Alcontres 31, 98166 Messina, Italy.
authors.forename: Ersilia
authors.initials: E
authors.lastname: Bellocco
mesh_terms: D000110:Acetylcholinesterase / Q000032:analysis; D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification; D001628:Beverages / Q000032:analysis*; D002800:Cholinesterase Inhibitors / Q000737:chemistry* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D032084:Citrus sinensis / Q000737:chemistry*; D005419:Flavonoids / Q000737:chemistry* / Q000302:isolation & purification; D005638:Fruit / Q000737:chemistry; D006801:Humans; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification; D053719:Tandem Mass Spectrometry
chemical_list: D000975:Antioxidants; D002800:Cholinesterase Inhibitors; D005419:Flavonoids; D010936:Plant Extracts; D000110:Acetylcholinesterase
keywords: Acetylcholinesterase activity; Antioxidant activity; Citrus sinensis (L.) Osbeck; Flavonoids; HPLC–DAD–ESI-MS-MS; Sanguinello and Tarocco blood orange