title: Photoinhibition of Phaeocystis globosa resulting from oxidative stress induced by a marine algicidal bacterium Bacillus sp. LP-10.
abstract: Harmful algal blooms caused by Phaeocystis globosa have resulted in staggering losses to coastal countries because of their world-wide distribution. Bacteria have been studied for years to control the blooms of harmful alga, however, the action mechanism of them against harmful algal cells is still not well defined. Here, a previously isolated algicidal bacterium Bacillus sp. LP-10 was used to elucidate the potential mechanism involved in the dysfunction of P. globosa algal cells at physiological and molecular levels. Our results showed Bacillus sp. LP-10 induced an obvious rise of reactive oxygen species (ROS), which was supposed to be major reason for algal cell death. Meanwhile, the results revealed a significant decrease of photosynthetic physiological indexes and apparent down-regulated of photosynthesis-related genes (psbA and rbcS) and protein (PSII reaction center protein D1), after treated by Bacillus sp. LP-10 filtrates, suggesting photoinhibition occurred in the algal cells. Furthermore, our results indicated that light played important roles in the algal cell death. Our work demonstrated that the major lethal reason of P. globosa cells treated by the algicidal bacterium was the photoinhibition resulted from oxidative stress induced by Bacillus sp. LP-10.
authors.affiliation: State Key Laboratory of Marine Environmental Science and Key Laboratory of Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen 361102, China.|Tobacco Science Institute of Jiangxi Province, Nanchang 330025, China.
authors.forename: Chengwei
authors.initials: C
authors.lastname: Guan
authors.affiliation: State Key Laboratory of Marine Environmental Science and Key Laboratory of Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen 361102, China.|School of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361102, China.
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Marine Environmental Science and Key Laboratory of Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen 361102, China.|College of Life Sciences, Henan Normal University, Xinxiang 453007, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Li
authors.affiliation: State Key Laboratory of Marine Environmental Science and Key Laboratory of Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen 361102, China.
authors.forename: Huajun
authors.initials: H
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Marine Environmental Science and Key Laboratory of Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen 361102, China.
authors.forename: Xueqian
authors.initials: X
authors.lastname: Lei
authors.affiliation: State Key Laboratory of Marine Environmental Science and Key Laboratory of Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen 361102, China.
authors.forename: Guanjing
authors.initials: G
authors.lastname: Cai
authors.affiliation: State Key Laboratory of Marine Environmental Science and Key Laboratory of Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen 361102, China.
authors.forename: Jiajia
authors.initials: J
authors.lastname: Guo
authors.affiliation: Key Laboratory of Marine Ecology and Environmental Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Marine Environmental Science and Key Laboratory of Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen 361102, China.
authors.forename: Tianling
authors.initials: T
authors.lastname: Zheng
mesh_terms: D020418:Algal Proteins / Q000235:genetics / Q000378:metabolism; D000975:Antioxidants / Q000032:analysis; D001407:Bacillus / Q000502:physiology*; D002734:Chlorophyll / Q000032:analysis; D058087:Haptophyta / Q000254:growth & development* / Q000378:metabolism / Q000528:radiation effects; D008027:Light; D008315:Malondialdehyde / Q000032:analysis; D018384:Oxidative Stress / Q000502:physiology*; D010788:Photosynthesis; D045332:Photosystem II Protein Complex / Q000235:genetics / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D013050:Spectrometry, Fluorescence
chemical_list: D020418:Algal Proteins; D000975:Antioxidants; D045332:Photosystem II Protein Complex; D017382:Reactive Oxygen Species; D002734:Chlorophyll; D008315:Malondialdehyde