title: Expression Profiles of Estrogen-Regulated MicroRNAs in Breast Cancer Cells.
abstract: Molecular signaling through both estrogen and microRNAs are critical for breast cancer development and growth. The activity of estrogen is mediated by transcription factors, the estrogen receptors. Here we describe a method for robust characterization of estrogen-regulated microRNA profiles. The method details how to prepare cells for optimal estrogen response, directions for estrogen treatment, RNA extraction, microRNA large-scale profiling, and subsequent confirmations.
authors.affiliation: Center for Nuclear Receptors and Cell Signaling, Department of Biology and Biochemistry, University of Houston, Houston, TX, 77204-5056, USA.
authors.forename: Anne
authors.initials: A
authors.lastname: Katchy
authors.affiliation: Department of Biology and Biochemistry, Center for Nuclear Receptors and Cell Signaling, University of Houston, Houston, TX, USA. cecilia.williams@scilifelab.se.|SciLifeLab, Department of Proteomics and Nanobiotechnology, KTH-Royal Institute of Technology, Solna, Sweden. cecilia.williams@scilifelab.se.
authors.forename: Cecilia
authors.initials: C
authors.lastname: Williams
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics* / Q000378:metabolism; D004958:Estradiol / Q000494:pharmacology*; D047628:Estrogen Receptor alpha / Q000819:agonists / Q000378:metabolism; D004967:Estrogens / Q000494:pharmacology*; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006801:Humans; D061986:MCF-7 Cells; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results; D013997:Time Factors; D057188:Workflow
chemical_list: C506487:ESR1 protein, human; D047628:Estrogen Receptor alpha; D004967:Estrogens; D035683:MicroRNAs; D004958:Estradiol
keywords: Breast cancer; Estrogen; Estrogen receptor; MicroRNA; Microarray; qPCR