title: CAR-T cells are serial killers.
abstract: Chimeric antigen receptor (CAR) T cells have enjoyed unprecedented clinical success against haematological malignancies in recent years. However, several aspects of CAR T cell biology remain unknown. We recently compared CAR and T cell receptor (TCR)-based killing in the same effector cell and showed that CAR T cells can not only efficiently kill single tumor targets, they can also kill multiple tumor targets in a sequential manner. Single and serial killing events were not sustained long term due to CAR down-regulation after 20 hours.
authors.affiliation: Cancer Immunology Research; Peter MacCallum Cancer Center , East Melbourne, VIC Australia ; Sir Peter MacCallum Department of Oncology; University of Melbourne , Parkville, VIC Australia ; The ACRF Translational Research Laboratory; Royal Melbourne Hospital , Parkville, VIC Australia.
authors.forename: Alexander J
authors.initials: AJ
authors.lastname: Davenport
authors.affiliation: Cancer Immunology Research; Peter MacCallum Cancer Center , East Melbourne, VIC Australia ; Sir Peter MacCallum Department of Oncology; University of Melbourne , Parkville, VIC Australia.
authors.forename: Misty R
authors.initials: MR
authors.lastname: Jenkins
authors.affiliation: Cancer Immunology Research; Peter MacCallum Cancer Center , East Melbourne, VIC Australia ; Sir Peter MacCallum Department of Oncology; University of Melbourne , Parkville, VIC Australia ; The ACRF Translational Research Laboratory; Royal Melbourne Hospital , Parkville, VIC Australia.
authors.forename: David S
authors.initials: DS
authors.lastname: Ritchie
authors.affiliation: Department of Cancer Medicine; Peter MacCallum Cancer Center , East Melbourne, VIC Australia.
authors.forename: H Miles
authors.initials: HM
authors.lastname: Prince
authors.affiliation: Cancer Immunology Research; Peter MacCallum Cancer Center , East Melbourne, VIC Australia ; Sir Peter MacCallum Department of Oncology; University of Melbourne , Parkville, VIC Australia.
authors.forename: Joseph A
authors.initials: JA
authors.lastname: Trapani
authors.affiliation: Cancer Immunology Research; Peter MacCallum Cancer Center , East Melbourne, VIC Australia ; Sir Peter MacCallum Department of Oncology; University of Melbourne , Parkville, VIC Australia.
authors.forename: Michael H
authors.initials: MH
authors.lastname: Kershaw
authors.affiliation: Cancer Immunology Research; Peter MacCallum Cancer Center , East Melbourne, VIC Australia ; Sir Peter MacCallum Department of Oncology; University of Melbourne , Parkville, VIC Australia.
authors.forename: Phillip K
authors.initials: PK
authors.lastname: Darcy
authors.affiliation: Cancer Immunology Research; Peter MacCallum Cancer Center , East Melbourne, VIC Australia ; Sir Peter MacCallum Department of Oncology; University of Melbourne , Parkville, VIC Australia.
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Neeson
keywords: CAR; T cell therapy; serial killing