title: Preparation and evaluation of a molecularly imprinted sol-gel material as the solid-phase extraction adsorbents for the specific recognition of cloxacilloic acid in cloxacillin.
abstract: Highly selective molecularly imprinted polymers on the surface of silica gels were prepared by a sol-gel process and used as solid-phase extraction adsorbents for the specific recognition, enrichment and detection of cloxacilloic acid in cloxacillin. The obtained polymers were characterized by scanning electron microscopy, FTIR spectroscopy, nitrogen adsorption and desorption, elemental analysis and thermogravimetric analysis. The imprinted polymers not only possessed high adsorption capacity (6.5 μg/mg), but also exhibited fast adsorption kinetics (they adsorb 80% of the maximum amount within 20 min) and excellent selectivity (the imprinted factor was 3.6). A method using the imprinted polymers as solid-phase extraction adsorbents coupled with high-performance liquid chromatography was established with good specificity, linearity (r = 0.9962), precision (ranging from 0.5 to 6.7%), accuracy (ranging from 93.9 to 97.7%) and extraction recoveries (ranging from 78.8 to 89.8%). The limits of detection and quantification were 0.07 and 0.25 mg/g, respectively. This work could provide a promising method in the enrichment, extraction and detection of allergenic impurities in the manufacture, storage and application of cloxacillin.
authors.affiliation: School of Pharmacy, Xi'an Jiaotong University, Xi'an, China.|Department of Pharmacy, Tianjin Huanhu Hospital, Tianjin, China.
authors.forename: Kangli
authors.initials: K
authors.lastname: Du
authors.affiliation: School of Pharmacy, Xi'an Jiaotong University, Xi'an, China.
authors.forename: Zhimin
authors.initials: Z
authors.lastname: Luo
authors.affiliation: School of Pharmacy, Xi'an Jiaotong University, Xi'an, China.
authors.forename: Pengqi
authors.initials: P
authors.lastname: Guo
authors.affiliation: School of Pharmacy, Xi'an Jiaotong University, Xi'an, China.|Department of Pharmacy, Hospital of Stomatology Attached Xi'an Jiaotong University, Xi'an, China.
authors.forename: Weili
authors.initials: W
authors.lastname: Tang
authors.affiliation: School of Pharmacy, Xi'an Jiaotong University, Xi'an, China.|Department of Pharmacy, Xi'an First Hospital, Xi'an, China.
authors.forename: Ningli
authors.initials: N
authors.lastname: Wu
authors.affiliation: School of Pharmacy, Xi'an Jiaotong University, Xi'an, China.
authors.forename: Penglei
authors.initials: P
authors.lastname: Zheng
authors.affiliation: School of Pharmacy, Xi'an Jiaotong University, Xi'an, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Du
authors.affiliation: School of Pharmacy, Xi'an Jiaotong University, Xi'an, China.
authors.forename: Aiguo
authors.initials: A
authors.lastname: Zeng
authors.affiliation: School of Pharmacy, Xi'an Jiaotong University, Xi'an, China.
authors.forename: Wanghui
authors.initials: W
authors.lastname: Jing
authors.affiliation: School of Pharmacy, Xi'an Jiaotong University, Xi'an, China.
authors.forename: Chun
authors.initials: C
authors.lastname: Chang
authors.affiliation: School of Pharmacy, Xi'an Jiaotong University, Xi'an, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Fu
mesh_terms: D000327:Adsorption; D003023:Cloxacillin / Q000737:chemistry*; D005069:Evaluation Studies as Topic; D006801:Humans; D008855:Microscopy, Electron, Scanning; D054802:Molecular Imprinting; D052616:Solid Phase Extraction
chemical_list: D003023:Cloxacillin
keywords: Allergenic impurities; Cloxacillin; Molecularly imprinted polymers; Sol-gel process; Solid-phase extraction