title: Patient-Derived Human Induced Pluripotent Stem Cells From Gingival Fibroblasts Composited With Defined Nanohydroxyapatite/Chitosan/Gelatin Porous Scaffolds as Potential Bone Graft Substitutes.
abstract: Human embryonic stem cells and adult stem cells have always been the cell source for bone tissue engineering. However, their limitations are obvious, including ethical concerns and/or a short lifespan. The use of human induced pluripotent stem cells (hiPSCs) could avoid these problems. Nanohydroxyapatite (nHA) is an important component of natural bone and bone tissue engineering scaffolds. However, its regulation on osteogenic differentiation with hiPSCs from human gingival fibroblasts (hGFs) is unknown. The purpose of the present study was to investigate the osteogenic differentiation of hiPSCs from patient-derived hGFs regulated by nHA/chitosan/gelatin (HCG) scaffolds with different nHA ratios, such as HCG-111 (1 wt/vol% nHA) and HCG-311 (3 wt/vol% nHA). First, hGFs were reprogrammed into hiPSCs, which have enhanced osteogenic differentiation capability. Second, HCG-111 and HCG-311 scaffolds were successfully synthesized. Finally, hiPSC/HCG complexes were cultured in vitro or subcutaneously transplanted into immunocompromised mice in vivo. The osteogenic differentiation effects of two types of HCG scaffolds on hiPSCs were assessed for up to 12 weeks. The results showed that HCG-311 increased osteogenic-related gene expression of hiPSCs in vitro proved by quantitative real-time polymerase chain reaction, and hiPSC/HCG-311 complexes formed much bone-like tissue in vivo, indicated by cone-beam computed tomography imaging, H&E staining, Masson staining, and RUNX-2, OCN immunohistochemistry staining. In conclusion, our study has shown that osteogenic differentiation of hiPSCs from hGFs was improved by HCG-311. The mechanism might be that the nHA addition stimulates osteogenic marker expression of hiPSCs from hGFs. Our work has provided an innovative autologous cell-based bone tissue engineering approach with soft tissues such as clinically abundant gingiva.
  
  
  CONCLUSIONS
  The present study focused on patient-personalized bone tissue engineering. Human induced pluripotent stem cells (hiPSCs) were established from clinically easily derived human gingival fibroblasts (hGFs) and defined nanohydroxyapatite/chitosan/gelatin (HCG) scaffolds. hiPSCs derived from hGFs had better osteogenesis capability than that of hGFs. More interestingly, osteogenic differentiation of hiPSCs from hGFs was elevated significantly when composited with HCG-311 scaffolds in vitro and in vivo. The present study has uncovered the important role of different nHA ratios in HCG scaffolds in osteogenesis induction of hiPSCs derived from hGFs. This technique could serve as a potential innovative approach for bone tissue engineering, especially large bone regeneration clinically.
authors.affiliation: Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, People's Republic of China Nanjing Key Laboratory, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, People's Republic of China.
authors.forename: Jun
authors.initials: J
authors.lastname: Ji
authors.affiliation: Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, People's Republic of China.
authors.forename: Xin
authors.initials: X
authors.lastname: Tong
authors.affiliation: Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, People's Republic of China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, People's Republic of China.
authors.forename: Junfeng
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, People's Republic of China Nanjing Key Laboratory, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, People's Republic of China haiyanandrew@163.com qghu@nju.edu.cn.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Qin
authors.affiliation: Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, People's Republic of China haiyanandrew@163.com qghu@nju.edu.cn.
authors.forename: Qingang
authors.initials: Q
authors.lastname: Hu
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D018786:Bone Substitutes / Q000494:pharmacology*; D048271:Chitosan / Q000494:pharmacology*; D017886:Durapatite / Q000494:pharmacology*; D005260:Female; D005347:Fibroblasts / Q000378:metabolism / Q000473:pathology / Q000637:transplantation; D005780:Gelatin / Q000494:pharmacology*; D005881:Gingiva / Q000378:metabolism / Q000473:pathology; D064593:Heterografts; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000378:metabolism / Q000473:pathology / Q000637:transplantation; D008297:Male; D051379:Mice; D008819:Mice, Nude; D010012:Osteogenesis / Q000187:drug effects; D016062:Porosity; D054457:Tissue Scaffolds
chemical_list: D018786:Bone Substitutes; D005780:Gelatin; D048271:Chitosan; D017886:Durapatite
keywords: Autologous stem cell transplantation; Differentiation; Induced pluripotent stem cells; Osteoblast; Reprogramming; Tissue regeneration