title: Enrichment of Circulating and Mucosal Cytotoxic CD8+ T Cells Is Associated with Postoperative Endoscopic Recurrence in Patients with Crohn's Disease.
abstract: OBJECTIVE
  Evidence from mouse colitis models indicates that cytotoxic CD8+ T cells [CTL] play a key role in the initiation of gut lesions. We investigated whether changes in CD8+ CTL in blood or lamina propria [LP] of the neoterminal ileum were associated with postoperative endoscopic recurrence of Crohn's disease [CD].
  
  
  METHODS
  A total of 37 CD patients with ileocolonic resection were endoscopically followed up at 6 and 12 months post-surgery. CD8+ T cells were analysed by flow cytometry in blood and ileal LP.
  
  
  RESULTS
  Granzyme B- and perforin-producing CD8+ T cells were significantly increased at 6 months in blood and in ileum LP in patients with endoscopic recurrence, as compared with those in remission. At a cutoff point of 45% of CD8+ CTL, the overall accuracies of the frequency of blood granzyme B+ or perforin+ CD8+ T cells to identify patients with postoperative endoscopic recurrence were 77% and 83%, respectively. Interestingly, patients with endoscopic recurrence at 12 months were those showing the highest mucosal CD8+ CTL frequency at 6 months, while still in remission.
  
  
  CONCLUSIONS
  Enrichment of cytotoxic CD8+ T cells in blood and ileal mucosa coincides with CD postoperative endoscopic recurrence. This underscores that CD8 CTL may play a pathophysiological role in the initiation of gut lesions during CD.
authors.affiliation: International Center for Infectiology Research, Université de Lyon, Lyon, France INSERM U1111, Team Immunity, Vaccination and Biotherapy, Lyon, France Université Claude Bernard Lyon 1, Lyon, France Department of Gastroenterology, Centre Hospitalier Lyon Sud, Pierre-Bénite, France.
authors.forename: Gilles
authors.initials: G
authors.lastname: Boschetti
authors.affiliation: International Center for Infectiology Research, Université de Lyon, Lyon, France INSERM U1111, Team Immunity, Vaccination and Biotherapy, Lyon, France Université Claude Bernard Lyon 1, Lyon, France Department of Gastroenterology, Centre Hospitalier Lyon Sud, Pierre-Bénite, France.
authors.forename: Stephane
authors.initials: S
authors.lastname: Nancey
authors.affiliation: Department of Gastroenterology, Centre Hospitalier Lyon Sud, Pierre-Bénite, France.
authors.forename: Driffa
authors.initials: D
authors.lastname: Moussata
authors.affiliation: Department of Surgery, Centre Hospitalier Lyon Sud, Pierre-Bénite, France.
authors.forename: Eddy
authors.initials: E
authors.lastname: Cotte
authors.affiliation: Department of Surgery, Centre Hospitalier Lyon Sud, Pierre-Bénite, France.
authors.forename: Yves
authors.initials: Y
authors.lastname: Francois
authors.affiliation: International Center for Infectiology Research, Université de Lyon, Lyon, France INSERM U1111, Team Immunity, Vaccination and Biotherapy, Lyon, France Université Claude Bernard Lyon 1, Lyon, France Department of Gastroenterology, Centre Hospitalier Lyon Sud, Pierre-Bénite, France.
authors.forename: Bernard
authors.initials: B
authors.lastname: Flourié
authors.affiliation: International Center for Infectiology Research, Université de Lyon, Lyon, France INSERM U1111, Team Immunity, Vaccination and Biotherapy, Lyon, France Université Claude Bernard Lyon 1, Lyon, France.
authors.forename: Dominique
authors.initials: D
authors.lastname: Kaiserlian
mesh_terms: D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D003082:Colectomy; D003113:Colonoscopy; D003424:Crohn Disease / Q000000981:diagnostic imaging / Q000276:immunology* / Q000601:surgery*; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007082:Ileum / Q000276:immunology* / Q000601:surgery; D007413:Intestinal Mucosa / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies; D012008:Recurrence; D013602:T-Lymphocytes, Cytotoxic / Q000378:metabolism*; D016896:Treatment Outcome
keywords: CD8+ cytotoxic T cells; Crohn’s disease; Postoperative recurrence