title: Cancer Gastric Chemoprevention: Isolation of Gastric Tumor-Initiating Cells.
abstract: Gastric cancer is an important healthcare problem and represents the second leading cause of death for malignant disease worldwide. In the Western world, the diagnosis is done at late stage when treatments can be only palliative. Searches for new therapeutic regimens as well as for new biomarkers are in progress. To reduce cancer mortality is crucial the prevention of the lesion at earlier stages. Therefore, new bullets to prevention are needed. Nowadays, studies relating to different kinds of tumor are unanimous in considering cancer stem cells (CSCs) as "the core" of the tumor and the responsible of tumor chemoresistance and relapse. This chapter aims to provide the instructions to (1) isolate, (2) grow, and (3) validate, both in vivo and in vitro, the gastric CSC subpopulation.
authors.affiliation: Molecular Chemoprevention Unit, Molecular Medicine Department, Regina Elena National Cancer Institute, via Elio Chianesi, 53, 00144, Rome, Italy. f.mori@ifo.it.
authors.forename: Federica
authors.initials: F
authors.lastname: Mori
authors.affiliation: Translational Oncogenomic Unit, Molecular Medicine Department, Regina Elena National Cancer Institute, Rome, Italy.
authors.forename: Valeria
authors.initials: V
authors.lastname: Canu
authors.affiliation: Surgical and Medical Department of Translational Medicine, Faculty of Medicine and Psychology, Sant'Andrea Hospital, University of Rome "La Sapienza", Rome, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Lorenzon
authors.affiliation: Department of GI Surgery, Regina Elena National Cancer Institute, Rome, Italy.
authors.forename: Alfredo
authors.initials: A
authors.lastname: Garofalo
authors.affiliation: Laboratory of Translational Oncogenomics, Molecular Medicine Department, Regina Elena National Cancer Institute, Rome, Italy.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Blandino
authors.affiliation: Molecular Cancer Chemoprevention Unit, Molecular Medicine Department, Regina Elena National Cancer Institute, via Elio Chianesi, 53, 00144, Rome, Italy.|Department of Oncology, Juravinski Cancer Center-McMaster University Hamilton, Hamilton, ON, Italy.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Strano
mesh_terms: D000818:Animals; D063646:Carcinogenesis / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002469:Cell Separation / Q000379:methods*; D018890:Chemoprevention; D019008:Drug Resistance, Neoplasm; D005260:Female; D006801:Humans; D051379:Mice; D014411:Neoplastic Stem Cells / Q000187:drug effects / Q000473:pathology*; D013274:Stomach Neoplasms / Q000473:pathology* / Q000517:prevention & control*
keywords: Cancer stem cells; Chemoprevention; Chemoresistance; Gastric cancer; Patient-derived xenograft