title: Eosinophils from Murine Lamina Propria Induce Differentiation of Naïve T Cells into Regulatory T Cells via TGF-β1 and Retinoic Acid.
abstract: Treg cells play a crucial role in immune tolerance, but mechanisms that induce Treg cells are poorly understood. We here have described eosinophils in lamina propria (LP) that displayed high aldehyde dehydrogenase (ALDH) activity, a rate-limiting step during all-trans retinoic acid (ATRA) synthesis, and expressed TGF-β1 mRNA and high levels of ATRA. Co-incubation assay confirmed that LP eosinophils induced the differentiation of naïve T cells into Treg cells. Differentiation promoted by LP eosinophils were inhibited by blocked either TGF-β1 or ATRA. Peripheral blood (PB) eosinophils did not produce ATRA and could not induce Treg differentiation. These data identifies LP eosinophils as effective inducers of Treg cell differentiation through a mechanism dependent on TGF-β1 and ATRA.
authors.affiliation: Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310003, P.R. China.|Division of Basic Medical Microbiology, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310058, P.R. China.|Department of Medical Microbiology and Parasitology, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310058, P.R. China.|Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, Zhejiang, 310051, P.R.China.
authors.forename: Hong-Hu
authors.initials: HH
authors.lastname: Chen
authors.affiliation: Faculty of Basic Medicine, Zhejiang Medical College, Hangzhou, Zhejiang, 310053, P.R. China.
authors.forename: Ai-Hua
authors.initials: AH
authors.lastname: Sun
authors.affiliation: Health Sciences Research Institute and Molecular Cell Biology, University of California, Merced, California, 95343, United States of America.
authors.forename: David M
authors.initials: DM
authors.lastname: Ojcius
authors.affiliation: Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310003, P.R. China.|Division of Basic Medical Microbiology, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310058, P.R. China.|Department of Medical Microbiology and Parasitology, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310058, P.R. China.
authors.forename: Wei-Lin
authors.initials: WL
authors.lastname: Hu
authors.affiliation: Division of Basic Medical Microbiology, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310058, P.R. China.|Department of Medical Microbiology and Parasitology, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310058, P.R. China.
authors.forename: Yu-Mei
authors.initials: YM
authors.lastname: Ge
authors.affiliation: Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310003, P.R. China.|Division of Basic Medical Microbiology, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310058, P.R. China.|Department of Medical Microbiology and Parasitology, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310058, P.R. China.
authors.forename: Xu'ai
authors.initials: X
authors.lastname: Lin
authors.affiliation: Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310003, P.R. China.|Division of Basic Medical Microbiology, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310058, P.R. China.
authors.forename: Lan-Juan
authors.initials: LJ
authors.lastname: Li
authors.affiliation: Department of Clinical Medicine, School of Medicine, Zhejiang University City College, Hangzhou, Zhejiang, 310015, P.R. China.
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Pan
authors.affiliation: Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310003, P.R. China.|Division of Basic Medical Microbiology, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310058, P.R. China.|Department of Medical Microbiology and Parasitology, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310058, P.R. China.
authors.forename: Jie
authors.initials: J
authors.lastname: Yan
mesh_terms: D000444:Aldehyde Dehydrogenase / Q000096:biosynthesis* / Q000276:immunology; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D002454:Cell Differentiation / Q000276:immunology; D004804:Eosinophils / Q000276:immunology / Q000378:metabolism; D007108:Immune Tolerance; D008213:Lymphocyte Activation / Q000276:immunology; D051379:Mice; D009092:Mucous Membrane / Q000276:immunology / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000166:cytology* / Q000276:immunology; D053773:Transforming Growth Factor beta1 / Q000096:biosynthesis* / Q000276:immunology; D014212:Tretinoin / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D053773:Transforming Growth Factor beta1; D014212:Tretinoin; D000444:Aldehyde Dehydrogenase