title: Effect of electronic stimulation at Neiguan (PC 6) acupoint on gene expression of adenosine triphosphate-sensitive potassium channel and protein kinases in rats with myocardial ischemia.
abstract: OBJECTIVE
  To investigate the effects of electronic stimulation at acupoints Neiguan (PC 6) and Lieque (LU 7) on the gene expression of the adenosine triphosphate (ATP)-Sensitive potassium channel (KATP: Kir6.1, Kir6.2, SUR2A, and SUR2B) and protein kinases (PKA, PKG, and PKCβ2) in myocardial cells of rats with myocardial ischemia (MI) induced by isoproterenol (ISO).
  
  
  METHODS
  Rats were randomly divided into a control, model, Neiguan (PC 6), Lieque (LU 7), and non-acupoint groups. The MI model was established by injecting rats with ISO. Electro-acupuncture treatment was given to the acupuncture groups, once a day for 7 days. Gene expression was analyzed with real-time PCR.
  
  
  RESULTS
  The gene expression of KATP and protein kinases in the model group was higher than those in the control group (P < 0.05). After acupuncture treatment, the KATP and protein kinase expression levels were significantly lower in the Neiguan (PC 6) and Lieque (LU 7) groups compared with the mod- el group (P < 0.05). The Neiguan (PC 6) group lowered these levels significantly more than that of the Lieque (LU 7) group (P < 0.05). No significant differences were observed between the model and non-acupoint groups (P> 0.05).
  
  
  CONCLUSIONS
  Our findings suggest that electronic needling of Neiguan (PC 6) can both reduce the gene expression of KATP and protein kinases in rats with ISO-induced MI.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Jiquan
authors.initials: J
authors.lastname: Li
authors.forename: Xiangyu
authors.initials: X
authors.lastname: Meng
authors.forename: Yiguo
authors.initials: Y
authors.lastname: Chen
mesh_terms: D015669:Acupuncture Points; D015670:Acupuncture Therapy; D000818:Animals; D015870:Gene Expression; D006801:Humans; D054086:KATP Channels / Q000235:genetics* / Q000378:metabolism; D008297:Male; D017202:Myocardial Ischemia / Q000235:genetics / Q000378:metabolism / Q000628:therapy*; D032383:Myocytes, Cardiac / Q000378:metabolism; D024661:Potassium Channels, Inwardly Rectifying / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D000099161:Kcnj11 Channel
chemical_list: D054086:KATP Channels; D024661:Potassium Channels, Inwardly Rectifying; D000099161:Kcnj11 Channel; C092520:uK-ATP-1 potassium channel