title: Effects of Nogo-A Silencing on TNF-α and IL-6 Secretion and TH Downregulation in Lipopolysaccharide-Stimulated PC12 Cells.
abstract: Parkinson's disease (PD) is a common degenerative disease that lacks efficient treatment. Myelin-associated neurite outgrowth inhibitor A (Nogo-A) is relevant with inhibition of nerve regeneration and may play vital role in pathogenesis of PD. The study aimed to establish the shRNA expression plasmids of Nogo-A gene and explore the regulatory effects of Nogo-A silencing on the expression of inflammation factor tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6) as well as tyrosine hydroxylase (TH) in lipopolysaccharide- (LPS-) stimulated rat PC12 cells. The results showed that both mRNA and protein levels of Nogo-A in pGenesil-nogoA-shRNA group were downregulated. The viabilities of PC12 cells decreased with increase of LPS concentrations. LPS significantly increased the supernatant TNF-alpha and IL-6 concentrations and reduced TH protein expression in PC12 cells, while silencing Nogo-A could block these effects. These results suggested that LPS can activate PC12 cells to secrete inflammatory cytokines and lower the TH expression, which can be regulated by Nogo-A gene silencing. Nogo-A silencing might provide new ideas for PD treatment in the future.
authors.affiliation: Neurology Department, People's Hospital of Zengcheng City (Boji Hospital Affiliated to Sun Yat-sen University), Guangzhou 511300, China.
authors.forename: Jianbin
authors.initials: J
authors.lastname: Zhong
authors.affiliation: Neurology Department, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China.
authors.forename: Shengnuo
authors.initials: S
authors.lastname: Fan
authors.affiliation: Neurology Department, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China.
authors.forename: Zhenwen
authors.initials: Z
authors.lastname: Yan
authors.affiliation: Neurology Department, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China.
authors.forename: Songhua
authors.initials: S
authors.lastname: Xiao
authors.affiliation: Neurology Department, People's Hospital of Zengcheng City (Boji Hospital Affiliated to Sun Yat-sen University), Guangzhou 511300, China.
authors.forename: Limei
authors.initials: L
authors.lastname: Wan
authors.affiliation: Neurology Department, People's Hospital of Zengcheng City (Boji Hospital Affiliated to Sun Yat-sen University), Guangzhou 511300, China.
authors.forename: Chibang
authors.initials: C
authors.lastname: Chen
authors.affiliation: Neurology Department, People's Hospital of Zengcheng City (Boji Hospital Affiliated to Sun Yat-sen University), Guangzhou 511300, China.
authors.forename: Simin
authors.initials: S
authors.lastname: Zhong
authors.affiliation: Neurology Department, People's Hospital of Zengcheng City (Boji Hospital Affiliated to Sun Yat-sen University), Guangzhou 511300, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Liu
authors.affiliation: Neurology Department, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
mesh_terms: D000818:Animals; D005786:Gene Expression Regulation / Q000187:drug effects; D020868:Gene Silencing; D006801:Humans; D007249:Inflammation / Q000139:chemically induced / Q000235:genetics* / Q000473:pathology; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000633:toxicity; D009185:Myelin Proteins / Q000037:antagonists & inhibitors / Q000235:genetics*; D009416:Nerve Regeneration / Q000235:genetics; D000070798:Nogo Proteins; D016716:PC12 Cells; D010300:Parkinson Disease / Q000235:genetics* / Q000473:pathology / Q000628:therapy; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism*; D014446:Tyrosine 3-Monooxygenase / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D015850:Interleukin-6; D008070:Lipopolysaccharides; D009185:Myelin Proteins; D000070798:Nogo Proteins; D012333:RNA, Messenger; C000603199:RTN4 protein, human; C000603197:Rtn4 protein, rat; D014409:Tumor Necrosis Factor-alpha; D014446:Tyrosine 3-Monooxygenase