title: Quality and fertilizing capacity of boar spermatozoa during liquid storage in extender supplemented with different antibiotics.
abstract: The aim of the study was to determine the effect of antibiotics on quality parameters and fertilizing capacity of boar sperm during liquid preservation. In the first experiment, semen was diluted in an extender containing 200 μg/mL of gentamicin as a control and diluted in a modified extenders: Ext I (contained 200 μg/mL florfenicol), Ext II (contained 200 μg/mL polymyxin B), Ext III (contained 100 μg/mL gentamicin and 100 μg/mL florfenicol) and Ext IV (contained 100 μg/mL gentamicin and 100 μg/mL polymyxin B). The semen was stored for ten days. Sperm quality was evaluated based on the motility (CASA; TM: total motility; PM: progressive motility), membrane integrity (YO-PRO-1/PI assay), mitochondrial activity (JC-1) and DNA integrity (TUNEL). The highest PM% (62.5 ± 9.6) was observed in Ext III at Day 6 of storage. The highest sperm viability and mitochondrial transmembrane potential was noticed at the end of the storage period in Ext III. Long-term storage did not induce DNA fragmentation in the extenders analyzed. In the second experiment, semen diluted in the control extender and in the extender providing the highest quality spermatozoa on Day 10 (Ext III) was used for artificial insemination (AI) of synchronized gilts. Our studies showed that the highest reproductive performance of inseminated gilts (pregnant gilts: 97.0%, litter size: 11.4 ± 1.2) occurred with Ext III semen dilution. The combination of 100 μg/mL gentamicin and 100 μg/mL florfenicol in the extender maintained sperm motility, membrane integrity and mitochondrial activity and enhanced the higher reproduction success.
authors.affiliation: National Research Institute of Animal Production, Department of Animal Reproduction, 32-083 Balice/Kraków, Poland. Electronic address: magdalena.bryla@izoo.krakow.pl.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Bryła
authors.affiliation: National Research Institute of Animal Production, Department of Animal Reproduction, 32-083 Balice/Kraków, Poland.
authors.forename: Monika
authors.initials: M
authors.lastname: Trzcińska
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D005260:Female; D005298:Fertility; D007315:Insemination, Artificial / Q000662:veterinary; D008297:Male; D055101:Semen Analysis / Q000662:veterinary*; D012662:Semen Preservation; D013094:Spermatozoa / Q000502:physiology*; D013552:Swine
chemical_list: D000900:Anti-Bacterial Agents
keywords: Antibiotics; Apoptotic-like changes; Boar spermatozoa; Gilts; Long-term storage; Reproductive performance