title: Simultaneous determination of 3-chlorotyrosine and 3-nitrotyrosine in human plasma by direct analysis in real time-tandem mass spectrometry.
abstract: A novel method for the simultaneous determination of 3-nitrotyrosine (NT) and 3-chlorotyrosine (CT) in human plasma has been developed based on direct analysis in real time-tandem mass spectrometry (DART-MS/MS). Analysis was performed in the positive ionization mode using multiple reaction monitoring (MRM) of the ion transitions at m/z 216.2/170.1 for CT, m/z 227.2/181.1 for NT and m/z 230.2/184.2 for the internal standard, d (3)-NT. The assay was linear in the ranges 0.5-100 μg/mL for CT and 4-100 μg/mL for NT with corresponding limits of detection of 0.2 and 2 μg/mL. Intra- and inter-day precisions and accuracies were respectively <15% and ±15%. Matrix effects were also evaluated. The method is potentially useful for high throughput analysis although sensitivity needs to be improved before it can be applied in clinical research.
authors.affiliation: Medical Experiment and Analysis Center of PLA General Hospital, Beijing 100853, China.
authors.forename: Yuqiao
authors.initials: Y
authors.lastname: Song
authors.affiliation: Medical Experiment and Analysis Center of PLA General Hospital, Beijing 100853, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Liao
authors.affiliation: Medical Experiment and Analysis Center of PLA General Hospital, Beijing 100853, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Zha
authors.affiliation: Medical Experiment and Analysis Center of PLA General Hospital, Beijing 100853, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.affiliation: ASPEC Technologies Limited Beijing, Beijing, 100102, China.
authors.forename: Charles C
authors.initials: CC
authors.lastname: Liu
keywords: 3-Chlorotyrosine; 3-Nitrotyrosine; DART–MS/MS; Determintion; Human plasma