title: Serum Biomarkers of (Anti)Oxidant Status for Epidemiological Studies.
abstract: In this review, we disclose a selection of serum/plasma biomarkers of (anti)oxidant status related to nutrition, which can be used for measurements in large-scale epidemiological studies. From personal experience, we have come to the following proposal of a set of biomarkers for nutritional intake, (anti)oxidant status, and redox status. We have selected the individual antioxidant vitamins E and A, and the carotenoids which can be measured in large series by HPLC. In addition, vitamin C was selected, which can be measured by an auto-analyzer or HPLC. As a biomarker for oxidative stress, the ROM assay (reactive oxygen metabolites) was selected; for the redox status, the total thiol assay; and for the total antioxidant status the BAP assay (biological antioxidant potential). All of these biomarkers can be measured in large quantities by an auto-analyzer. Critical points in biomarker validation with respect to blood sampling, storage conditions, and measurements are discussed. With the selected biomarkers, a good set is presented for use in the risk assessment between nutrition and (chronic) diseases in large-scale epidemiological studies. Examples of the successful application of these biomarkers in large international studies are presented.
authors.affiliation: Centre for Health Protection, National Institute for Public Health and the Environment, PO Box 1, 3720 BA Bilthoven, The Netherlands. eugene.jansen@rivm.nl.
authors.forename: Eugène
authors.initials: E
authors.lastname: Jansen
authors.affiliation: Faculty of Medical Sciences, Goce Delcev University, 2000 Stip, Macedonia. tatjana.ruskovska@ugd.edu.mk.
authors.forename: Tatjana
authors.initials: T
authors.lastname: Ruskovska
mesh_terms: D000975:Antioxidants; D015415:Biomarkers / Q000097:blood*; D016021:Epidemiologic Studies; D006801:Humans; D015227:Lipid Peroxidation; D009749:Nutrition Surveys; D016877:Oxidants / Q000097:blood*; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D062486:Public Health Surveillance; D014815:Vitamins / Q000097:blood
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D016877:Oxidants; D014815:Vitamins
keywords: antioxidant status; biomarkers; epidemiology; nutrition; oxidative stress; redox status; vitamins