title: The Arabidopsis a zinc finger domain protein ARS1 is essential for seed germination and ROS homeostasis in response to ABA and oxidative stress.
abstract: The phytohormone abscisic acid (ABA) induces accumulation of reactive oxygen species (ROS), which can disrupt seed dormancy and plant development. Here, we report the isolation and characterization of an Arabidopsis thaliana mutant called ars1 (aba and ros sensitive 1) that showed hypersensitivity to ABA during seed germination and to methyl viologen (MV) at the seedling stage. ARS1 encodes a nuclear protein with one zinc finger domain, two nuclear localization signal (NLS) domains, and one nuclear export signal (NES). The ars1 mutants showed reduced expression of a gene for superoxide dismutase (CSD3) and enhanced accumulation of ROS after ABA treatment. Transient expression of ARS1 in Arabidopsis protoplasts strongly suppressed ABA-mediated ROS production. Interestingly, nuclear-localized ARS1 translocated to the cytoplasm in response to treatment with ABA, H2O2, or MV. Taken together, these results suggest that ARS1 modulates seed germination and ROS homeostasis in response to ABA and oxidative stress in plants.
authors.affiliation: Division of Applied Life Science (BK21 Plus Program), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University Jinju, South Korea.
authors.forename: Dongwon
authors.initials: D
authors.lastname: Baek
authors.affiliation: Division of Applied Life Science (BK21 Plus Program), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University Jinju, South Korea.
authors.forename: Joon-Yung
authors.initials: JY
authors.lastname: Cha
authors.affiliation: Division of Applied Life Science (BK21 Plus Program), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University Jinju, South Korea.
authors.forename: Songhwa
authors.initials: S
authors.lastname: Kang
authors.affiliation: Division of Applied Life Science (BK21 Plus Program), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University Jinju, South Korea.
authors.forename: Bokyung
authors.initials: B
authors.lastname: Park
authors.affiliation: Division of Applied Life Science (BK21 Plus Program), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University Jinju, South Korea.
authors.forename: Hyo-Jung
authors.initials: HJ
authors.lastname: Lee
authors.affiliation: Division of Applied Life Science (BK21 Plus Program), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University Jinju, South Korea.
authors.forename: Hyewon
authors.initials: H
authors.lastname: Hong
authors.affiliation: Division of Applied Life Science (BK21 Plus Program), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University Jinju, South Korea.
authors.forename: Hyun Jin
authors.initials: HJ
authors.lastname: Chun
authors.affiliation: College of Life Science and Natural Resources, Dong-A University Busan, South Korea.
authors.forename: Doh Hoon
authors.initials: DH
authors.lastname: Kim
authors.affiliation: Division of Applied Life Science (BK21 Plus Program), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University Jinju, South Korea.
authors.forename: Min Chul
authors.initials: MC
authors.lastname: Kim
authors.affiliation: Division of Applied Life Science (BK21 Plus Program), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University Jinju, South Korea.
authors.forename: Sang Yeol
authors.initials: SY
authors.lastname: Lee
authors.affiliation: Division of Applied Life Science (BK21 Plus Program), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University Jinju, South Korea.
authors.forename: Dae-Jin
authors.initials: DJ
authors.lastname: Yun
keywords: Arabidopsis; C2H2 zinc finger; abiotic stress; abscisic acid; reactive oxygen species; redox