title: Analysis of 30 insertion-deletion polymorphisms in the Japanese population using the Investigator DIPplex® kit.
abstract: Allele frequencies and forensic parameters for 30 insertion-deletion polymorphisms (INDELs) were investigated in a sample of 251 unrelated Japanese individuals using the Investigator DIPplex® kit (QIAGEN). The frequency distributions showed no deviations from Hardy-Weinberg equilibrium expectations. The combined powers of discrimination and match probability for the 30 INDELs were 0.9999999998 and 2.67×10(-11), respectively. To assess the effectiveness of the kit in typing degraded DNA, an ancient bone sample of a Jomon skeleton was analyzed; most of 30 INDELs and amelogenin were typed successfully. We concluded that the kit offers considerable potential for personal identification from degraded DNA samples due to the small amplicon length and high degree of polymorphisms.
authors.affiliation: Department of Legal Medicine, Shinshu University School of Medicine, Asahi 3-1-1, Matsumoto, Nagano 390-8621, Japan.
authors.forename: Miya
authors.initials: M
authors.lastname: Nunotani
authors.affiliation: Department of Legal Medicine, Shinshu University School of Medicine, Asahi 3-1-1, Matsumoto, Nagano 390-8621, Japan.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Shiozaki
authors.affiliation: Department of Legal Medicine, Shinshu University School of Medicine, Asahi 3-1-1, Matsumoto, Nagano 390-8621, Japan.
authors.forename: Noriko
authors.initials: N
authors.lastname: Sato
authors.affiliation: Department of Legal Medicine, Shinshu University School of Medicine, Asahi 3-1-1, Matsumoto, Nagano 390-8621, Japan.
authors.forename: Sayako
authors.initials: S
authors.lastname: Kamei
authors.affiliation: Department of Legal Medicine, Shinshu University School of Medicine, Asahi 3-1-1, Matsumoto, Nagano 390-8621, Japan.
authors.forename: Kanae
authors.initials: K
authors.lastname: Takatsu
authors.affiliation: Department of Legal Medicine, Shinshu University School of Medicine, Asahi 3-1-1, Matsumoto, Nagano 390-8621, Japan.
authors.forename: Tokutaro
authors.initials: T
authors.lastname: Hayashi
authors.affiliation: Department of Legal Medicine, Shinshu University School of Medicine, Asahi 3-1-1, Matsumoto, Nagano 390-8621, Japan.
authors.forename: Masao
authors.initials: M
authors.lastname: Ota
authors.affiliation: Department of Legal Medicine, Shinshu University School of Medicine, Asahi 3-1-1, Matsumoto, Nagano 390-8621, Japan. Electronic address: asamura@shinshu-u.ac.jp.
authors.forename: Hideki
authors.initials: H
authors.lastname: Asamura
mesh_terms: D005260:Female; D017353:Gene Deletion; D005828:Genetics, Population / Q000295:instrumentation*; D006801:Humans; D007564:Japan; D008297:Male; D011110:Polymorphism, Genetic
keywords: Degraded DNA; Insertion–deletion polymorphisms (INDELs); Investigator DIPplex® kit; Japanese population