title: Genotypic characterisation of human papillomavirus infections among persons living with HIV infection; a case-control study in Kumasi, Ghana.
abstract: OBJECTIVE
  The objective of this study is to describe the burden of human papillomavirus (HPV) infection among women living with HIV and non-infected women in Ghana.
  
  
  METHODS
  A case-control study was conducted involving 107 women living with HIV aged between 18 and 59 years (cases) and 100 non-HIV-infected apparently healthy women (controls) who were recruited from the Kumasi South Hospital, from July to December, 2014. Cervicovaginal swabs were taken from study participants to characterise 28 high- and low-risk HPV genotypes using a multiplex real-time PCR.
  
  
  RESULTS
  The overall mean age for the participants was 40.10 ± 9.76 years. The prevalence of high-risk (hr)-HPV genotypes was significantly higher among the cases than the controls (77.4% vs. 41.6%, P < 0.0001). Overall, HPV 58 and 54 were the most predominant high-risk (18.8%) and low-risk (15.0%) genotypes detected. The two most common hr-HPV genotype isolates were 58 (18.8%) and 35 (15.9%) with 58 being the most prevalent among age group 35-44 years compared with hr-HPV 16, 18, 35 and 45, found predominantly among 18-34 age group.
  
  
  CONCLUSIONS
  Significant variations exist in HPV genotypes among HIV-infected and uninfected women.
authors.affiliation: Kumasi Centre for Collaborative Research in Tropical Medicine, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
authors.forename: Denis Dekugmen
authors.initials: DD
authors.lastname: Yar
authors.affiliation: Kumasi Centre for Collaborative Research in Tropical Medicine, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.|Department of Biochemistry and Biotechnology, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
authors.forename: Samson Pandam
authors.initials: SP
authors.lastname: Salifu
authors.affiliation: Kumasi Centre for Collaborative Research in Tropical Medicine, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
authors.forename: Samuel Nkansah
authors.initials: SN
authors.lastname: Darko
authors.affiliation: Kumasi Centre for Collaborative Research in Tropical Medicine, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
authors.forename: Augustina Angelina
authors.initials: AA
authors.lastname: Annan
authors.affiliation: Ghana Health Service, Kumasi South Hospital, Ashanti Region, Ghana.
authors.forename: Akosua Adumea
authors.initials: AA
authors.lastname: Gyimah
authors.affiliation: Department of Clinical and Social Pharmacy, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
authors.forename: Kwame Ohene
authors.initials: KO
authors.lastname: Buabeng
authors.affiliation: Kumasi Centre for Collaborative Research in Tropical Medicine, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.|School of Public Health, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
authors.forename: Ellis
authors.initials: E
authors.lastname: Owusu-Dabo
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D005838:Genotype; D005869:Ghana / Q000453:epidemiology; D015658:HIV Infections / Q000150:complications*; D006801:Humans; D008875:Middle Aged; D027383:Papillomaviridae / Q000235:genetics*; D030361:Papillomavirus Infections / Q000150:complications / Q000453:epidemiology* / Q000821:virology; D015995:Prevalence; D060888:Real-Time Polymerase Chain Reaction; D002583:Uterine Cervical Neoplasms / Q000821:virology; D055815:Young Adult
chemical_list: D004279:DNA, Viral
keywords: Genotipos; Genotypes; Ghana; HIV; VIH; génotypes; human papillomavirus (HPV); virus del papiloma humano (VPH); virus du papillome humain (VPH)