title: Sodium glycididazole enhances the radiosensitivity of laryngeal cancer cells through downregulation of ATM signaling pathway.
abstract: The purpose of this study was to evaluate the radiation-enhancing effect of sodium glycididazole, and the corresponding mechanisms of action on laryngeal cancer cells. Two laryngeal cancer cell lines (Hep-2 and UT-SCC-19A) were irradiated with X-rays in the presence or absence of sodium glycididazole. Cell survival, DNA damage and repair, cell apoptosis, cell cycle distribution, expression of proteins related to cell cycle checkpoint, and apoptosis were measured. Significantly increased DNA damages, decreased cells in the G1 phase, arrested cells at G2/M phase, decreased DNA repair protein XRCC1 foci formation, and enhanced cell apoptosis were observed in laryngeal cell lines treated by sodium glycididazole combined with irradiation compared with the irradiation alone. The combined treatment downregulated the protein expressions of ataxia-telangiectasia mutated (ATM), p-ATM, CHK2, and P53 but upregulated the protein expressions of MDM2 and Cdk2. This study indicates that sodium glycididazole enhances the radiosensitivity of laryngeal cancer cells through downregulation of ATM signaling pathway in vitro and in vivo.
authors.affiliation: Department of Medical Oncology, Cancer Center, Shengjing Hospital of China Medical University, 39 Huaxiang Road, Shenyang, 110022, China.
authors.forename: Yue-Can
authors.initials: YC
authors.lastname: Zeng
authors.affiliation: Department of Medical Oncology, Cancer Center, Shengjing Hospital of China Medical University, 39 Huaxiang Road, Shenyang, 110022, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Xing
authors.affiliation: Department of Radiation Oncology, University of Washington School of Medicine, Seattle, WA, USA.
authors.forename: Jing
authors.initials: J
authors.lastname: Zeng
authors.affiliation: Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical University, 39 Huaxiang Road, Shenyang, 110022, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Xue
authors.affiliation: Department of Medical Oncology, Cancer Center, Shengjing Hospital of China Medical University, 39 Huaxiang Road, Shenyang, 110022, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Chi
authors.affiliation: Cancer Institute, No.1 Hospital of China Medical University, Shenyang, 110001, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Xin
authors.affiliation: Department of Otorhinolaryngology, Harbin First Hospital, 151, Diduan Street, Harbin, 150010, China.
authors.forename: Guo-Liang
authors.initials: GL
authors.lastname: Fan
authors.affiliation: Department of Radiation Oncology, Nanfang Hospital of Southern Medical University, Guangzhou, 510515, China.
authors.forename: Hong-Mei
authors.initials: HM
authors.lastname: Wang
authors.affiliation: Department of Medical Oncology, Cancer Center, Shengjing Hospital of China Medical University, 39 Huaxiang Road, Shenyang, 110022, China.
authors.forename: Qiong-Yu
authors.initials: QY
authors.lastname: Duan
authors.affiliation: Department of Medical Oncology, Cancer Center, Shengjing Hospital of China Medical University, 39 Huaxiang Road, Shenyang, 110022, China.
authors.forename: Yu-Nan
authors.initials: YN
authors.lastname: Sun
authors.affiliation: Department of Medical Oncology, Cancer Center, Shengjing Hospital of China Medical University, 39 Huaxiang Road, Shenyang, 110022, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Niu
authors.affiliation: Department of Medical Oncology, Cancer Center, Shengjing Hospital of China Medical University, 39 Huaxiang Road, Shenyang, 110022, China. wellyy2005@hotmail.com.
authors.forename: Rong
authors.initials: R
authors.lastname: Wu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D064007:Ataxia Telangiectasia Mutated Proteins / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000378:metabolism; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004260:DNA Repair / Q000187:drug effects; D004195:Disease Models, Animal; D056945:Hep G2 Cells; D006801:Humans; D000860:Hypoxia / Q000188:drug therapy / Q000378:metabolism; D007093:Imidazoles / Q000494:pharmacology*; D007822:Laryngeal Neoplasms / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects*; D047368:Tumor Burden / Q000187:drug effects / Q000528:radiation effects; D023041:Xenograft Model Antitumor Assays
chemical_list: D014408:Biomarkers, Tumor; D007093:Imidazoles; D011838:Radiation-Sensitizing Agents; C000603632:sodium glycididazole; D064007:Ataxia Telangiectasia Mutated Proteins
keywords: ATM signaling pathway; Laryngeal cancer; Radiation-enhancing effect; Sodium glycididazole