title: Complete genome sequence of Novosphingobium pentaromativorans US6-1(T).
abstract: Novosphingobium pentaromativorans US6-1(T) is a species in the family Sphingomonadaceae. According to the phylogenetic analysis based on 16S rRNA gene sequence of the N. pentaromativorans US6-1(T) and nine genome-sequenced strains in the genus Novosphingobium, the similarity ranged from 93.9 to 99.9 % and the highest similarity was found with Novosphingobium sp. PP1Y (99.9 %), whereas the ANI value based on genomes ranged from 70.9 to 93 % and the highest value was 93 %. This microorganism was isolated from muddy coastal bay sediments where the environment is heavily polluted by polycyclic aromatic hydrocarbons (PAHs). It was previously shown to be capable of degrading multiple PAHs, including benzo[a]pyrene. To further understand the PAH biodegradation pathways the previous draft genome of this microorganism was revised to obtain a complete genome using Illumina MiSeq and PacBio platform. The genome of strain US6-1(T) consists of 5,457,578 bp, which includes the 3,979,506 bp chromosome and five megaplasmids. It comprises 5110 protein-coding genes and 82 RNA genes. Here, we provide an analysis of the complete genome sequence which enables the identification of new characteristics of this strain.
authors.affiliation: Marine Biotechnology Research Division, Korea Institute of Ocean Science and Technology, Ansan, 426-744 Republic of Korea.
authors.forename: Dong Hee
authors.initials: DH
authors.lastname: Choi
authors.affiliation: Marine Biotechnology Research Division, Korea Institute of Ocean Science and Technology, Ansan, 426-744 Republic of Korea.
authors.forename: Yong Min
authors.initials: YM
authors.lastname: Kwon
authors.affiliation: Marine Biotechnology Research Division, Korea Institute of Ocean Science and Technology, Ansan, 426-744 Republic of Korea.|Major of Marine Biotechnology, University of Science and Technology, Daejeon, 305-350 Republic of Korea.
authors.forename: Kae Kyoung
authors.initials: KK
authors.lastname: Kwon
authors.affiliation: Marine Biotechnology Research Division, Korea Institute of Ocean Science and Technology, Ansan, 426-744 Republic of Korea.|Major of Marine Biotechnology, University of Science and Technology, Daejeon, 305-350 Republic of Korea.|National Marine Biodiversity Institute of Korea, Seocheon, 325-902 Republic of Korea.
authors.forename: Sang-Jin
authors.initials: SJ
authors.lastname: Kim
keywords: Extradiol dioxygenase; Megaplasmids; Novosphingobium; Polycyclic aromatic hydrocarbon