title: Post-transcriptional modulation of interleukin 8 by CNOT6L regulates skeletal muscle differentiation.
abstract: CNOT6L is a deadenylase subunit belonging to the CCR4-NOT complex, a major deadenylase complex in eukaryotes involved at multiple levels in regulation of gene expression. While CNOT6L is expressed in skeletal muscle cells, its specific functions in this tissue are still largely unknown. Our previous work highlighted the functional of CNOT6L in skeletal muscle cell differentiation. To further explore how CNOT6L regulates myogenesis, we used here gene expression analysis to identify CNOT6L mRNA targets in human myoblasts. Among these novel targets, IL-8 (interleukin 8) mRNA was the most upregulated in CNOT6L knock-down (KD) cells. Biochemical approaches and poly (A) tail length assays showed that IL-8 mRNA is a direct target of CNOT6L, and further investigations by loss- and gain-of-function assays pointed out that IL-8 is an important effector of myogenesis. Therefore, we have characterized CNOT6L-IL-8 as a new signaling axis that regulates myogenesis.
authors.affiliation: Institute for Integrative Biology of the Cell (I2BC), IBiTecs, CEA, CNRS, Univ Paris Saclay, Gif-sur-Yvette F-91198, France.
authors.forename: Anna
authors.initials: A
authors.lastname: Polesskaya
authors.affiliation: Institute for Integrative Biology of the Cell (I2BC), IBiTecs, CEA, CNRS, Univ Paris Saclay, Gif-sur-Yvette F-91198, France.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Pinna
authors.affiliation: Institute of Pharmacology and Toxicology, Technische Universitaet Muenchen (TUM), Biedersteiner Strasse 29, 80802 Munich, Germany.
authors.forename: Yassine
authors.initials: Y
authors.lastname: Sassi
authors.affiliation: Institute for Integrative Biology of the Cell (I2BC), IBiTecs, CEA, CNRS, Univ Paris Saclay, Gif-sur-Yvette F-91198, France.
authors.forename: Marie
authors.initials: M
authors.lastname: Vandamme
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06, INSERM UMRS974, CNRS FRE3617, Center for Research in Myology, 47 Boulevard de l'hôpital, 75013 Paris, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Bigot
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06, INSERM UMRS974, CNRS FRE3617, Center for Research in Myology, 47 Boulevard de l'hôpital, 75013 Paris, France.
authors.forename: Vincent
authors.initials: V
authors.lastname: Mouly
authors.affiliation: Institute for Integrative Biology of the Cell (I2BC), IBiTecs, CEA, CNRS, Univ Paris Saclay, Gif-sur-Yvette F-91198, France.
authors.forename: Nadya
authors.initials: N
authors.lastname: Morozova
authors.affiliation: Institute for Integrative Biology of the Cell (I2BC), IBiTecs, CEA, CNRS, Univ Paris Saclay, Gif-sur-Yvette F-91198, France.
authors.forename: Annick
authors.initials: A
authors.lastname: Harel-Bellan
authors.affiliation: Institute for Integrative Biology of the Cell (I2BC), IBiTecs, CEA, CNRS, Univ Paris Saclay, Gif-sur-Yvette F-91198, France. Electronic address: cindy.degerny@u-psud.fr.
authors.forename: Cindy
authors.initials: C
authors.lastname: Degerny
mesh_terms: D000328:Adult; D000818:Animals; D015153:Blotting, Western; D002454:Cell Differentiation / Q000235:genetics*; D002460:Cell Line; D002478:Cells, Cultured; D020869:Gene Expression Profiling; D006801:Humans; D016209:Interleukin-8 / Q000235:genetics* / Q000378:metabolism; D008856:Microscopy, Fluorescence; D024510:Muscle Development / Q000235:genetics; D018485:Muscle Fibers, Skeletal / Q000166:cytology / Q000378:metabolism; D018482:Muscle, Skeletal / Q000166:cytology / Q000378:metabolism*; D032446:Myoblasts / Q000166:cytology / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012260:Ribonucleases / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: C508606:CXCL8 protein, human; D016209:Interleukin-8; D012333:RNA, Messenger; C521732:CNOT6L protein, human; D012260:Ribonucleases
keywords: CNOT6L; Differentiation; IL-8; Myogenesis; Post-transcriptional regulation