title: Significance of twist and iNOS expression in human breast carcinoma.
abstract: Twist is a basic helix-loop-helix transcription factor family normally expressed during embryonic development and apparently activated in variety of tumours. Overexpression of twist is correlated with uncontrolled cell proliferation, differentiation, invasion and metastasis. Twist expression is associated with oestrogen receptor (ER); however, the molecular mechanism behind involvement of twist in progression of breast cancer is still unclear. Nitric oxide synthases (NOSs) which cause damage to the cellular DNA are also shown to be involved in cancer progression. The present study involves total number of n = 85 breast biopsies, which include 19 non-cancer and 66 cancerous lesions. We analysed twist, iNOS and ER expression pattern in human breast carcinomas by RT-PCR and also analysed twist cellular localisation by immunohistochemical analysis. iNOS expression pattern was correlated with different stages of breast carcinoma. Twist expression was significantly increased in cancer lesions when compared to the non-cancer. The breast cancer lesions positive to ER showed positivity to twist (72%) as well. The higher stages of cancer lesions showed a significant expression of twist localised in cytoplasm of the cancer cells. Collectively these data indicate that up-regulation of twist is correlated with the ER presenting breast cancer, and iNOS expression was positively correlated with tumour-node metastasis (TNM) staging of breast cancer. These findings suggest that expression of twist and iNOS may have a functional role in cancer progression.
authors.affiliation: Department of Biochemistry, University of Madras, Guindy campus, Chennai, Tamil Nadu, India. santhalaxmi@gmail.com.
authors.forename: Santhalakshmi
authors.initials: S
authors.lastname: Ranganathan
authors.affiliation: Department of Surgery, Madras Medical College, Chennai, Tamil Nadu, India.
authors.forename: Arunkumar
authors.initials: A
authors.lastname: Krishnan
authors.affiliation: Department of Biochemistry, University of Madras, Guindy campus, Chennai, Tamil Nadu, India.
authors.forename: Niranjali Devaraj
authors.initials: ND
authors.lastname: Sivasithambaram
mesh_terms: D000328:Adult; D000368:Aged; D001943:Breast Neoplasms / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D008875:Middle Aged; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism*; D051879:Twist-Related Protein 1 / Q000378:metabolism*; D055815:Young Adult
chemical_list: D009687:Nuclear Proteins; C103173:TWIST1 protein, human; D051879:Twist-Related Protein 1; D052247:Nitric Oxide Synthase Type II
keywords: Breast cancer; Oestrogen receptor; Twist; iNOS