title: Mir-302c mediates influenza A virus-induced IFNβ expression by targeting NF-κB inducing kinase.
abstract: Little is known about the role of microRNA during influenza A virus (IAV) infection. We observed that NIK 3'UTR luciferase activity was elevated during IAV infection. Further studies demonstrated that miR-302c reduced NIK expression, resulting in the reduction of IFNβ mRNA expression. We found that miR-302c prevented the translocation of NF-κB from the cytosol to the nucleus. Furthermore, IAV infection downregulated miR-302c expression, leading to the activation of IFNβ expression and the inhibition of viral replication. Compared to miR-302c, miR-520e cannot promote viral replication and production, although the two microRNAs target the same site of the NIK 3'UTR. Collectively, our work defines a novel signaling pathway implicated in the control of IFNβ mRNA expression during IAV infection.
authors.affiliation: State Key Laboratory of Virology and College of Life Sciences, Wuhan University, Wuhan 430072, China. Electronic address: guishulin2013@163.com.
authors.forename: Shulin
authors.initials: S
authors.lastname: Gui
authors.affiliation: State Key Laboratory of Virology and College of Life Sciences, Wuhan University, Wuhan 430072, China. Electronic address: chenxueyuan@whu.edu.cn.
authors.forename: Xueyuan
authors.initials: X
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Virology and College of Life Sciences, Wuhan University, Wuhan 430072, China. Electronic address: 913955307@qq.com.
authors.forename: Mo
authors.initials: M
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Virology and College of Life Sciences, Wuhan University, Wuhan 430072, China. Electronic address: fxz111@case.edu.
authors.forename: Fanpeng
authors.initials: F
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Virology and College of Life Sciences, Wuhan University, Wuhan 430072, China. Electronic address: yswan@whu.edu.cn.
authors.forename: Yushun
authors.initials: Y
authors.lastname: Wan
authors.affiliation: State Key Laboratory of Virology and College of Life Sciences, Wuhan University, Wuhan 430072, China. Electronic address: fflashddd@163.com.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Virology and College of Life Sciences, Wuhan University, Wuhan 430072, China. Electronic address: jmdsqs@gmail.com.
authors.forename: Gang
authors.initials: G
authors.lastname: Xu
authors.affiliation: Animal Biosafety Level III Laboratory at the Center for Animal Experiment, State Key Laboratory of Virology, Wuhan University, Wuhan 430071, China. Electronic address: zhouli_jerry@whu.edu.cn.
authors.forename: Li
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Clinical Research Center, Wuhan Children's Hospital, Wuhan 430016, China. Electronic address: bioyuexin@126.com.
authors.forename: Xin
authors.initials: X
authors.lastname: Yue
authors.affiliation: State Key Laboratory of Virology and College of Life Sciences, Wuhan University, Wuhan 430072, China. Electronic address: yingzhu@whu.edu.cn.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Virology and College of Life Sciences, Wuhan University, Wuhan 430072, China. Electronic address: liushi_liushi@whu.edu.cn.
authors.forename: Shi
authors.initials: S
authors.lastname: Liu
mesh_terms: D020413:3' Untranslated Regions; D021581:Active Transport, Cell Nucleus; D045744:Cell Line, Tumor; D004794:Enzyme Repression; D005786:Gene Expression Regulation; D054884:Host-Pathogen Interactions; D006801:Humans; D007113:Immunity, Innate; D018928:Immunity, Mucosal; D053122:Influenza A Virus, H3N2 Subtype / Q000276:immunology* / Q000502:physiology; D050838:Interferon Regulatory Factor-3 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D050839:Interferon Regulatory Factor-7 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D016899:Interferon-beta / Q000819:agonists / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D035683:MicroRNAs / Q000378:metabolism*; D016328:NF-kappa B / Q000819:agonists / Q000037:antagonists & inhibitors / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D020545:Respiratory Mucosa / Q000201:enzymology / Q000276:immunology* / Q000378:metabolism / Q000821:virology; D020218:Response Elements; D015398:Signal Transduction; D014779:Virus Replication; D000097782:NF-kappaB-Inducing Kinase
chemical_list: D020413:3' Untranslated Regions; D050838:Interferon Regulatory Factor-3; D050839:Interferon Regulatory Factor-7; D016899:Interferon-beta; D035683:MicroRNAs; D016328:NF-kappa B; D017346:Protein Serine-Threonine Kinases; D012333:RNA, Messenger; D000097782:NF-kappaB-Inducing Kinase; C494232:IRF3 protein, human; C494234:IRF7 protein, human; C532169:MIRN302A microRNA, human; C575048:MIRN520 microRNA, human
keywords: Influenza A virus; Innate immune; MiR-302c; NF-κB-inducing kinase; Type I IFN