title: Selective oxidation of UDP-glucose to UDP-glucuronic acid using permeabilized Schizosaccharomyces pombe expressing human UDP-glucose 6-dehydrogenase.
abstract: OBJECTIVE
  To use permeabilized cells of the fission yeast, Schizosaccharomyces pombe, that expresses human UDP-glucose 6-dehydrogenase (UGDH, EC 1.1.1.22), for the production of UDP-glucuronic acid from UDP-glucose.
  
  
  RESULTS
  In cell extracts no activity was detected. Therefore, cells were permeabilized with 0.3 % (v/v) Triton X-100. After washing away all low molecular weight metabolites, the permeabilized cells were directly used as whole cell biocatalyst. Substrates were 5 mM UDP-glucose and 10 mM NAD(+). Divalent cations were not added to the reaction medium as they promoted UDP-glucose hydrolysis. With this reaction system 5 mM UDP-glucose were converted into 5 mM UDP-glucuronic acid within 3 h.
  
  
  CONCLUSIONS
  Recombinant permeabilized cells of S. pombe can be used to synthesize UDP-glucuronic acid with 100 % yield and selectivity.
authors.affiliation: Biochemical Engineering Institute, Saarland University, Campus A1.5, 66123, Saarbrücken, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Weyler
authors.affiliation: School of Pharmaceutical Science and Technology (SPST), Tianjin University, Building 24, 92 Weijin Road, Nankai District, Tianjin, 300072, People's Republic of China.
authors.forename: Matthias
authors.initials: M
authors.lastname: Bureik
authors.affiliation: Biochemical Engineering Institute, Saarland University, Campus A1.5, 66123, Saarbrücken, Germany. e.heinzle@mx.uni-saarland.de.
authors.forename: Elmar
authors.initials: E
authors.lastname: Heinzle
mesh_terms: D003902:Detergents / Q000378:metabolism; D005954:Glucosephosphate Dehydrogenase / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D017830:Octoxynol / Q000378:metabolism; D010084:Oxidation-Reduction; D010539:Permeability / Q000187:drug effects; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012568:Schizosaccharomyces / Q000187:drug effects / Q000378:metabolism*; D014532:Uridine Diphosphate Glucose / Q000378:metabolism*; D014535:Uridine Diphosphate Glucuronic Acid / Q000378:metabolism*
chemical_list: D003902:Detergents; D011994:Recombinant Proteins; D014535:Uridine Diphosphate Glucuronic Acid; D017830:Octoxynol; D005954:Glucosephosphate Dehydrogenase; D014532:Uridine Diphosphate Glucose
keywords: Biocatalysis; Permeabilized cells; Schizosaccharomyces pombe; Selective oxidation; UDP-glucose-6-dehydrogenase; UDP-glucuronate