title: Regulation of the TMEPAI promoter by TCF7L2: the C-terminal tail of TCF7L2 is essential to activate the TMEPAI gene.
abstract: We previously found that TCF7L2 could activate the TMEPAI gene efficiently, whereas LEF1 could not nearly augment its transcription. When we comprehended the functional difference(s) between TCF7L2 and LEF1 with respect to the activation of the TMEPAI gene, the C-terminal tail of TCF7L2 was needed to reveal its transcriptional activity as well as its interaction with Smad3. Consistently, both TCF7/TCF7L2 and LEF1/TCF7L2 chimeric proteins exhibited an activity similar to TCF7L2 in transcription and Smad3 binding in contrast with LEF1 and TCF7. Our data elaborated on the diverse activity among TCF/LEF family members with respect to the transcriptional regulation of the TMEPAI gene.
authors.affiliation: Laboratory of Biochemistry, Showa Pharmaceutical University, 3-3165 Higashi-Tamagawagakuen, Machida, Tokyo 194-8543, Japan; Laboratory of Experimental Pathology, Graduate School of Comprehensive Human Sciences and Faculty of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8577, Japan.
authors.forename: Naoko
authors.initials: N
authors.lastname: Nakano
authors.affiliation: Laboratory of Experimental Pathology, Graduate School of Comprehensive Human Sciences and Faculty of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8577, Japan.
authors.forename: Mitsuyasu
authors.initials: M
authors.lastname: Kato
authors.affiliation: Laboratory of Biochemistry, Showa Pharmaceutical University, 3-3165 Higashi-Tamagawagakuen, Machida, Tokyo 194-8543, Japan; sitoh@ac.shoyaku.ac.jp.
authors.forename: Susumu
authors.initials: S
authors.lastname: Itoh
mesh_terms: D024742:HMG-Box Domains; D056945:Hep G2 Cells; D006801:Humans; D051821:Lymphoid Enhancer-Binding Factor 1 / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D020218:Response Elements; D051900:Smad3 Protein / Q000378:metabolism*; D058118:Transcription Factor 7-Like 2 Protein / Q000378:metabolism*; D015533:Transcriptional Activation; D060449:Wnt Signaling Pathway
chemical_list: C497573:LEF1 protein, human; D051821:Lymphoid Enhancer-Binding Factor 1; D008565:Membrane Proteins; C412868:PMEPA1 protein, human; C497790:SMAD3 protein, human; D051900:Smad3 Protein; C544894:TCF7L2 protein, human; D058118:Transcription Factor 7-Like 2 Protein
keywords: C-clamp; LEF family; Smad3; TCF; TGF-β/TMEPAI