title: TRIM59 Promotes the Proliferation and Migration of Non-Small Cell Lung Cancer Cells by Upregulating Cell Cycle Related Proteins.
abstract: TRIM protein family is an evolutionarily conserved gene family implicated in a number of critical processes including inflammation, immunity, antiviral and cancer. In an effort to profile the expression patterns of TRIM superfamily in several non-small cell lung cancer (NSCLC) cell lines, we found that the expression of 10 TRIM genes including TRIM3, TRIM7, TRIM14, TRIM16, TRIM21, TRIM22, TRIM29, TRIM59, TRIM66 and TRIM70 was significantly upregulated in NSCLC cell lines compared with the normal human bronchial epithelial (HBE) cell line, whereas the expression of 7 other TRIM genes including TRIM4, TRIM9, TRIM36, TRIM46, TRIM54, TRIM67 and TRIM76 was significantly down-regulated in NSCLC cell lines compared with that in HBE cells. As TRIM59 has been reported to act as a proto-oncogene that affects both Ras and RB signal pathways in prostate cancer models, we here focused on the role of TRIM59 in the regulation of NSCLC cell proliferation and migration. We reported that TRIM59 protein was significantly increased in various NSCLC cell lines. SiRNA-induced knocking down of TRIM59 significantly inhibited the proliferation and migration of NSCLC cell lines by arresting cell cycle in G2 phase. Moreover, TRIM59 knocking down affected the expression of a number of cell cycle proteins including CDC25C and CDK1. Finally, we knocked down TRIM59 and found that p53 protein expression levels did not upregulate, so we proposed that TRIM59 may promote NSCLC cell growth through other pathways but not the p53 signaling pathway.
authors.affiliation: Institute of Translation Medicine, Nanchang University, Nanchang City, Jiangxi, 330031, China.
authors.forename: Weihua
authors.initials: W
authors.lastname: Zhan
authors.affiliation: Institute of Translation Medicine, Nanchang University, Nanchang City, Jiangxi, 330031, China.
authors.forename: Tianyu
authors.initials: T
authors.lastname: Han
authors.affiliation: Institute of Translation Medicine, Nanchang University, Nanchang City, Jiangxi, 330031, China.
authors.forename: Chenfu
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Institute of Translation Medicine, Nanchang University, Nanchang City, Jiangxi, 330031, China.
authors.forename: Caifeng
authors.initials: C
authors.lastname: Xie
authors.affiliation: Institute of Translation Medicine, Nanchang University, Nanchang City, Jiangxi, 330031, China.
authors.forename: Mingxi
authors.initials: M
authors.lastname: Gan
authors.affiliation: Institute of Translation Medicine, Nanchang University, Nanchang City, Jiangxi, 330031, China.
authors.forename: Keyu
authors.initials: K
authors.lastname: Deng
authors.affiliation: Department of Basic Medical Science, School of Medicine, University of Missouri Kansas City, Kansas City, MO, 64108, United States of America.
authors.forename: Mingui
authors.initials: M
authors.lastname: Fu
authors.affiliation: Institute of Translation Medicine, Nanchang University, Nanchang City, Jiangxi, 330031, China.
authors.forename: Jian-Bin
authors.initials: JB
authors.lastname: Wang
mesh_terms: D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000473:pathology; D018797:Cell Cycle Proteins / Q000096:biosynthesis / Q000235:genetics*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins / Q000096:biosynthesis / Q000235:genetics*; D008667:Metalloproteins / Q000096:biosynthesis / Q000235:genetics*; D000090063:Proto-Oncogene Mas; D034741:RNA, Small Interfering; D015398:Signal Transduction / Q000235:genetics; D015533:Transcriptional Activation / Q000235:genetics; D000070584:Tripartite Motif Proteins; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D018797:Cell Cycle Proteins; D047908:Intracellular Signaling Peptides and Proteins; C000715470:MAS1 protein, human; D008565:Membrane Proteins; D008667:Metalloproteins; D000090063:Proto-Oncogene Mas; D034741:RNA, Small Interfering; C495901:TP53 protein, human; C530864:TRIM59 protein, human; D000070584:Tripartite Motif Proteins; D016159:Tumor Suppressor Protein p53