title: [Searching Radiation Countermeasures using the Model of Prolonged Irradiation of Mice with Low Dose Rate and Evaluation of Their Influence on Heat Shock Protein Genes Expression].
abstract: Different radiomodificators (cytokine betaleukine, antioxidant phenoxan, antigipoksant limontar and nucleoside riboxin) were investigated on mice for evaluating their radiation protective capacity against prolonged (21 h) exposure at a dose of 12.6 Gy at a low dose rate of 10 mGy/min. Bone marrow cellularity and endogenic CFUs were used as evaluation criteria 9 days after exposure. Simultaneously, expression of the heat shock proteins of 25, 70 and 90 kDa in unexposed mice bone marrow was studied 2, 24 and 48 h after injections. Betaleukine only had a positive significant effect in both tests in the variants of 50 mcg/kg and 3 mcg/kg when administered 2 h and 22 h before exposure, correspondingly. Effects of betaleukine HSPs on expression were both stimulating and inhibiting, that was in contradiction with a constant positive effect in 5 experiments on exposed mice for each betaleukine variant. It argues against the vital role of HSPs in the betaleukine antiradiation effect. In 2 experiments with high temperatures betaleukine administered at a dose of 50 mcg/kg evoked a very high HSP-70 gene expression after 24 h, and mice exposed to irradiation at that time in a parallel experiment showed an increased radiation effect. It corresponds to the idea that HSPs serve a stress indicator.
authors.forename: L M
authors.initials: LM
authors.lastname: Rozhdestvensky
authors.forename: V F
authors.initials: VF
authors.lastname: Mikhailov
authors.forename: T G
authors.initials: TG
authors.lastname: Schlyakova
authors.forename: J M
authors.initials: JM
authors.lastname: Shagirova
authors.forename: R A
authors.initials: RA
authors.lastname: Shchegoleva
authors.forename: N F
authors.initials: NF
authors.lastname: Raeva
authors.forename: N I
authors.initials: NI
authors.lastname: Lisina
authors.forename: L V
authors.initials: LV
authors.lastname: Shulenina
authors.forename: V V
authors.initials: VV
authors.lastname: Zorin
authors.forename: A V
authors.initials: AV
authors.lastname: Pchelka
authors.forename: K Y
authors.initials: KY
authors.lastname: Trubitsina
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000187:drug effects* / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D055808:Drug Discovery; D005720:Gamma Rays; D015870:Gene Expression / Q000187:drug effects* / Q000528:radiation effects; D006360:Heat-Shock Proteins / Q000235:genetics*; D008297:Male; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D011829:Radiation Dosage; D011833:Radiation Injuries, Experimental / Q000235:genetics / Q000378:metabolism / Q000517:prevention & control*; D011837:Radiation-Protective Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D060888:Real-Time Polymerase Chain Reaction; D013997:Time Factors; D014916:Whole-Body Irradiation
chemical_list: D006360:Heat-Shock Proteins; D011837:Radiation-Protective Agents