title: Urinary Sex Steroids and Anthropometric Markers of Puberty - A Novel Approach to Characterising Within-Person Changes of Puberty Hormones.
abstract: OBJECTIVE
  The longitudinal relationships of within-individual hormone and anthropometric changes during puberty have not ever been fully described. The objectives of this study were to demonstrate that 3 monthly urine collection was feasible in young adolescents and to utilise liquid chromatography-tandem mass spectrometry assay methods for serum and urine testosterone (T), estradiol (E2) and luteinizing hormone (LH) in adolescents by relating temporal changes in urine and serum hormones over 12 months to standard measures of pubertal development.
  
  
  METHODS
  A community sample of 104 adolescents (57 female) was studied over 12 months with annual anthropometric assessment, blood sampling and self-rated Tanner staging and urine collected every 3 months. Serum and urine sex steroids (T, E2) were measured by liquid chromatography-tandem mass spectrometry (LC-MS/MS) and LH by immunoassay.
  
  
  RESULTS
  A high proportion (92%) of scheduled samples were obtained with low attrition rate of 6.7% over the 12 months. Urine hormone measurements correlated cross-sectionally and longitudinally with age, anthropometry and Tanner stage.
  
  
  CONCLUSIONS
  We have developed a feasible and valid sampling methodology and measurements for puberty hormones in urine, which allows a sampling frequency by which individual pubertal progression in adolescents can be described in depth.
authors.affiliation: ANZAC Research Institute, University of Sydney, Camperdown, NSW, Australia.|Faculty of Pharmacy, Universiti Teknologi MARA, Selangor, Malaysia.
authors.forename: Gurmeet K S
authors.initials: GK
authors.lastname: Singh
authors.affiliation: Academic Department of Adolescent Medicine, Children's Hospital at Westmead, Westmead, NSW, Australia.|Discipline of Paediatrics and Child Health, Sydney Medical School, University of Sydney, Camperdown, NSW, Australia.
authors.forename: Ben W R
authors.initials: BW
authors.lastname: Balzer
authors.affiliation: Sydney School of Public Health, Sydney Medical School, University of Sydney, Camperdown, NSW, Australia.
authors.forename: Patrick J
authors.initials: PJ
authors.lastname: Kelly
authors.affiliation: School of Rural Health, Sydney Medical School, University of Sydney, Camperdown, NSW, Australia.
authors.forename: Karen
authors.initials: K
authors.lastname: Paxton
authors.affiliation: School of Rural Health, Sydney Medical School, University of Sydney, Camperdown, NSW, Australia.
authors.forename: Catherine I
authors.initials: CI
authors.lastname: Hawke
authors.affiliation: ANZAC Research Institute, University of Sydney, Camperdown, NSW, Australia.
authors.forename: David J
authors.initials: DJ
authors.lastname: Handelsman
authors.affiliation: Academic Department of Adolescent Medicine, Children's Hospital at Westmead, Westmead, NSW, Australia.|Discipline of Paediatrics and Child Health, Sydney Medical School, University of Sydney, Camperdown, NSW, Australia.
authors.forename: Katharine S
authors.initials: KS
authors.lastname: Steinbeck
mesh_terms: D000293:Adolescent; D002648:Child; D002853:Chromatography, Liquid; D005260:Female; D005640:Follicle Stimulating Hormone / Q000652:urine; D006801:Humans; D007986:Luteinizing Hormone / Q000652:urine; D008297:Male; D011627:Puberty / Q000652:urine*; D012741:Sexual Maturation / Q000502:physiology; D053719:Tandem Mass Spectrometry; D013739:Testosterone / Q000652:urine
chemical_list: D013739:Testosterone; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone