title: A novel near-infrared fluorescent platform with good photostability and the application for a reaction-based Cu(2+) probe in living cells.
abstract: Most of the near-infrared (NIR) fluorescent copper ion probes are coordination-based, and the fluorescence enhancement is between 10 and 20 folds. Herein, a novel NIR fluorescent dye named CSCN with excellent photostability and a reaction-based Cu(2+) NIR probe named CSCN-Cu were reported. CSCN exhibited good photostability toward photo irradiation. CSCN-Cu showed lower background fluorescent interference and over 40-fold fluorescence enhancement in NIR region, it also exhibited good selectivity toward Cu(2+) in Hepes solution. Biotic experiments demonstrated that CSCN-Cu possessed low toxicity and successfully imaged Cu(2+) in living cells under the conditions performed.
authors.affiliation: Institute of Fluorescent Probes for Biological Imaging, School of Chemistry and Chemical Engineering, School of Biological Science and Technology, University of Jinan, Jinan, Shandong 250022, PR China.
authors.forename: Keyin
authors.initials: K
authors.lastname: Liu
authors.affiliation: Institute of Fluorescent Probes for Biological Imaging, School of Chemistry and Chemical Engineering, School of Biological Science and Technology, University of Jinan, Jinan, Shandong 250022, PR China.
authors.forename: Huiming
authors.initials: H
authors.lastname: Shang
authors.affiliation: Institute of Fluorescent Probes for Biological Imaging, School of Chemistry and Chemical Engineering, School of Biological Science and Technology, University of Jinan, Jinan, Shandong 250022, PR China.
authors.forename: Fangfang
authors.initials: F
authors.lastname: Meng
authors.affiliation: Institute of Fluorescent Probes for Biological Imaging, School of Chemistry and Chemical Engineering, School of Biological Science and Technology, University of Jinan, Jinan, Shandong 250022, PR China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Institute of Fluorescent Probes for Biological Imaging, School of Chemistry and Chemical Engineering, School of Biological Science and Technology, University of Jinan, Jinan, Shandong 250022, PR China. Electronic address: weiyinglin2013@163.com.
authors.forename: Weiying
authors.initials: W
authors.lastname: Lin
mesh_terms: D000818:Animals; D002264:Carboxylic Acids / Q000737:chemistry; D002470:Cell Survival; D003300:Copper / Q000737:chemistry*; D004355:Drug Stability; D005456:Fluorescent Dyes / Q000737:chemistry*; D007259:Infrared Rays; D051379:Mice; D041681:NIH 3T3 Cells; D055620:Optical Phenomena; D012235:Rhodamines / Q000737:chemistry
chemical_list: D002264:Carboxylic Acids; D005456:Fluorescent Dyes; D012235:Rhodamines; D003300:Copper
keywords: Cell imaging; Cu(2+) ion; Near-infrared fluorescence; Photostability; Reaction-based probe