title: Optimization of ultrasound-assisted extraction of phenolic compounds from Cimicifugae rhizoma with response surface methodology.
abstract: BACKGROUND
  Cimicifugae rhizoma was a Ranunculaceae herb belonging to the composite family, and the roots of C. rhizoma have been widely used in tradition Chinese medicine.
  
  
  METHODS
  Ultrasound-assisted extraction (UAE) of phenolic compounds from C. rhizoma. Caffeic acid (CA), isoferulic acid (IA), ferulic acid (FA), and total phenols were quantified by high-performance liquid chromatography-diode array detection and ultraviolet-visible spectrophotometer. Effects of several experimental parameters, such as ultrasonic power (W), extraction temperature (°C), and ethanol concentration (%) on extraction efficiencies of phenolic compounds from C. rhizoma were evaluated.
  
  
  RESULTS
  The results showed that the optimal UAE condition was obtained with ultrasonic power of 377.35 W, extraction temperature of 70°C, and ethanol concentration of 58.37% for total phenols, and ultrasonic power of 318.28 W, extraction temperature of 59.65°C, and ethanol concentration of 64.43% for combination of CA, IA, FA.
  
  
  CONCLUSIONS
  The experimental values under optimal conditions were in good consistent with the predicted values, which suggested UAE is more efficient for the extraction of phenolic compounds from plant materials.
authors.affiliation: Department of Pharmacy, Dahua Hospital, Xuhui District, Shanghai 200237, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Liu
authors.affiliation: Engineering Research Center of Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing 210029, China ; Nanjing Haichang Chinese Medicine Group Co. Ltd, Nanjing 210061, China ; Key Laboratory of State Administration of TCM for Standardization of Chinese Medicine Processing, Nanjing 210023, PR China, China.
authors.forename: Bao-Jia
authors.initials: BJ
authors.lastname: Shen
authors.affiliation: Department of Pharmacy, Dahua Hospital, Xuhui District, Shanghai 200237, China.
authors.forename: Dong-Hao
authors.initials: DH
authors.lastname: Xie
authors.affiliation: Engineering Research Center of Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing 210029, China ; Nanjing Haichang Chinese Medicine Group Co. Ltd, Nanjing 210061, China ; Key Laboratory of State Administration of TCM for Standardization of Chinese Medicine Processing, Nanjing 210023, PR China, China.
authors.forename: Bao-Chang
authors.initials: BC
authors.lastname: Cai
authors.affiliation: Engineering Research Center of Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing 210029, China ; Nanjing Haichang Chinese Medicine Group Co. Ltd, Nanjing 210061, China ; Key Laboratory of State Administration of TCM for Standardization of Chinese Medicine Processing, Nanjing 210023, PR China, China.
authors.forename: Kun-Ming
authors.initials: KM
authors.lastname: Qin
authors.affiliation: Engineering Research Center of Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing 210029, China ; Key Laboratory of State Administration of TCM for Standardization of Chinese Medicine Processing, Nanjing 210023, PR China, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Cai
keywords: Cimicifugae Rhizome; phenolic compounds; response surface methodology; ultrasound-assisted extraction