title: Diffusion coefficients and dissociation constants of enhanced green fluorescent protein binding to free standing membranes.
abstract: Recently, a new and versatile assay to determine the partitioning coefficient [Formula: see text] as a measure for the affinity of peripheral membrane proteins for lipid bilayers was presented in the research article entitled, "Introducing a fluorescence-based standard to quantify protein partitioning into membranes" [1]. Here, the well-characterized binding of hexahistidine-tag (His6) to NTA(Ni) was utilized. Complementarily, this data article reports the average diffusion coefficient [Formula: see text] of His6-tagged enhanced green fluorescent protein (eGFP-His6) and the fluorescent lipid analog ATTO-647N-DOPE in giant unilamellar vesicles (GUVs) containing different amounts of NTA(Ni) lipids. In addition, dissociation constants [Formula: see text] of the NTA(Ni)/eGFP-His6 system are reported. Further, a conversion between [Formula: see text] and [Formula: see text] is provided.
authors.affiliation: Max Planck Institute of Biochemistry, Am Klopferspitz 18, D-82152 Martinsried, Germany.
authors.forename: Franziska A
authors.initials: FA
authors.lastname: Thomas
authors.affiliation: Max Planck Institute of Biochemistry, Am Klopferspitz 18, D-82152 Martinsried, Germany.
authors.forename: Ilaria
authors.initials: I
authors.lastname: Visco
authors.affiliation: Max Planck Institute of Biochemistry, Am Klopferspitz 18, D-82152 Martinsried, Germany ; Graz University of Technology, Institute of Biotechnology and Biochemical Engineering, Petersgasse 10-12/I, A-8010 Graz, Austria.
authors.forename: Zdeněk
authors.initials: Z
authors.lastname: Petrášek
authors.affiliation: Max Planck Institute of Biochemistry, Am Klopferspitz 18, D-82152 Martinsried, Germany ; Roche Diagnostics, Nonnenwald 2, D-82377 Penzberg, Germany.
authors.forename: Fabian
authors.initials: F
authors.lastname: Heinemann
authors.affiliation: Max Planck Institute of Biochemistry, Am Klopferspitz 18, D-82152 Martinsried, Germany.
authors.forename: Petra
authors.initials: P
authors.lastname: Schwille