title: Running per se stimulates the dendritic arbor of newborn dentate granule cells in mouse hippocampus in a duration-dependent manner.
abstract: Laboratory rodents provided chronic unlimited access to running wheels display increased neurogenesis in the hippocampal dentate gyrus. In addition, recent studies indicate that such an access to wheels stimulates dendritic arborization in newly formed neurons. However, (i) the presence of the running wheel in the housing environment might also bear intrinsic influences on the number and shape of new neurons and (ii) the dendritic arborization of new neurons might be insensitive to moderate daily running activity (i.e., several hours). In keeping with these uncertainties, we have examined neurogenesis and dendritic arborization in newly formed granular cells in adult C57Bl/6N male mice housed for 3 weeks under standard conditions, with a locked wheel, with a running wheel set free 3 h/day, or with a running wheel set permanently free. The results indicate that the presence of a blocked wheel in the home cage increased cell proliferation, but not the number of new neurons while running increased in a duration-dependent manner the number of newborn neurons, as assessed by DCX labeling. Morphological analyses of the dendritic tree of newborn neurons, as identified by BrdU-DCX co-staining, revealed that although the presence of the wheel stimulated their dendritic architecture, the amplitude of this effect was lower than that elicited by running activity, and was found to be running duration-dependent.
authors.affiliation: Endocannabinoids and NeuroAdaptation Group, Neurocentre Magendie, Bordeaux, France.|Neurogenesis and Physiopathology Group, NeuroCentre Magendie, Bordeaux, France.|Université Bordeaux Segalen, Bordeaux, France.
authors.forename: Sandrine
authors.initials: S
authors.lastname: Dostes
authors.affiliation: Endocannabinoids and NeuroAdaptation Group, Neurocentre Magendie, Bordeaux, France.|Université Bordeaux Segalen, Bordeaux, France.
authors.forename: Sarah
authors.initials: S
authors.lastname: Dubreucq
authors.affiliation: Neurogenesis and Physiopathology Group, NeuroCentre Magendie, Bordeaux, France.|Université Bordeaux Segalen, Bordeaux, France.
authors.forename: Elodie
authors.initials: E
authors.lastname: Ladevèze
authors.affiliation: Endocannabinoids and NeuroAdaptation Group, Neurocentre Magendie, Bordeaux, France.|Université Bordeaux Segalen, Bordeaux, France.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Marsicano
authors.affiliation: Neurogenesis and Physiopathology Group, NeuroCentre Magendie, Bordeaux, France.|Université Bordeaux Segalen, Bordeaux, France.
authors.forename: Djoher N
authors.initials: DN
authors.lastname: Abrous
authors.affiliation: Endocannabinoids and NeuroAdaptation Group, Neurocentre Magendie, Bordeaux, France.|Université Bordeaux Segalen, Bordeaux, France.
authors.forename: Francis
authors.initials: F
authors.lastname: Chaouloff
authors.affiliation: Neurogenesis and Physiopathology Group, NeuroCentre Magendie, Bordeaux, France.|Université Bordeaux Segalen, Bordeaux, France.
authors.forename: Muriel
authors.initials: M
authors.lastname: Koehl
mesh_terms: D000818:Animals; D001973:Bromodeoxyuridine / Q000378:metabolism; D049109:Cell Proliferation / Q000502:physiology*; D003712:Dendrites / Q000502:physiology*; D018891:Dentate Gyrus / Q000166:cytology*; D000089622:Doublecortin Domain Proteins; D000089662:Doublecortin Protein; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D055495:Neurogenesis / Q000502:physiology*; D009473:Neuronal Plasticity / Q000502:physiology; D009474:Neurons / Q000166:cytology* / Q000502:physiology; D009479:Neuropeptides / Q000378:metabolism; D010805:Physical Conditioning, Animal / Q000502:physiology; D012420:Running / Q000502:physiology*; D013997:Time Factors
chemical_list: C000714847:Dcx protein, mouse; D000089622:Doublecortin Domain Proteins; D000089662:Doublecortin Protein; D008869:Microtubule-Associated Proteins; D009479:Neuropeptides; D001973:Bromodeoxyuridine
keywords: adult neurogenesis; enrichment; exercise; hippocampus; mice; running wheel