title: The impact of temperature on microbial diversity and AOA activity in the Tengchong Geothermal Field, China.
abstract: Using a culture-independent method that combines CARD-FISH, qPCR and 16S rDNA, we investigated the abundance, community structure and diversity of microbes along a steep thermal gradient (50-90 °C) in the Tengchong Geothermal Field. We found that Bacteria and Archaea abundance changed markedly with temperature changes and that the number of cells was lowest at high temperatures (90.8 °C). Under low-temperature conditions (52.3-74.6 °C), the microbial communities were dominated by Bacteria, which accounted for 60-80% of the total number of cells. At 74.6 °C, Archaea were dominant, and at 90.8 °C, they accounted for more than 90% of the total number of cells. Additionally, the microbial communities at high temperatures (74.6-90.8 °C) were substantially simpler than those at the low-temperature sites. Only a few genera (e.g., bacterial Caldisericum, Thermotoga and Thermoanaerobacter, archaeal Vulcanisaeta and Hyperthermus) often dominated in high-temperature environments. Additionally, a positive correlation between Ammonia-Oxidizing Archaea (AOA) activity and temperature was detected. AOA activity increased from 17 to 52 pmol of NO2(-) per cell d(-1) with a temperature change from 50 to 70 °C.
authors.affiliation: School of Life Sciences and Technology, Tongji University, Shanghai 200092, China.
authors.forename: Haizhou
authors.initials: H
authors.lastname: Li
authors.affiliation: State Key Laboratory of Marine Geology, School of Ocean and Earth Science, Tongji University, Shanghai 200092, China.
authors.forename: Qunhui
authors.initials: Q
authors.lastname: Yang
authors.affiliation: School of Engineering, Anhui Agricultural University, Hefei 230000, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Li
authors.affiliation: State Key Laboratory of Marine Geology, School of Ocean and Earth Science, Tongji University, Shanghai 200092, China.
authors.forename: Hang
authors.initials: H
authors.lastname: Gao
authors.affiliation: School of Life Sciences and Technology, Tongji University, Shanghai 200092, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.affiliation: State Key Laboratory of Marine Geology, School of Ocean and Earth Science, Tongji University, Shanghai 200092, China.
authors.forename: Huaiyang
authors.initials: H
authors.lastname: Zhou
mesh_terms: D000641:Ammonia / Q000378:metabolism*; D001105:Archaea / Q000235:genetics / Q000378:metabolism*; D044822:Biodiversity; D002681:China; D019766:Genes, Archaeal; D017404:In Situ Hybridization, Fluorescence; D015345:Oligonucleotide Probes / Q000378:metabolism; D010084:Oxidation-Reduction; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology; D013696:Temperature
chemical_list: D015345:Oligonucleotide Probes; D012336:RNA, Ribosomal, 16S; D000641:Ammonia