title: The Induction of Growth Inhibition and Apoptosis in HeLa and MCF-7 Cells by Teucrium sandrasicum, Having Effective Antioxidant Properties.
abstract: The hidromethanolic (Met/W), ethyl acetate (EA(EA/W)), and water (W(EA/W)) extracts from Teucrium sandrasicum leaves (L) and flowers (F) were investigated for antioxidant properties and antiproliferative effects on HeLa, MCF-7, and L929. The highest DPPH scavenging, metal chelating capacities, and total phenolic and flavonoid contents were observed in Met/WL. The highest hydroxyl scavenging and reducing power capacities were found in EA(EA/W)L. Met/WL, EA(EA/W)L and EA(EA/W)F inhibited cancer cell growths, while they did not show significant cytotoxicity on L929. While the reactive oxygen species (ROS) levels were generally close to controls in HeLa, they were induced in MCF-7 with the treatment of Met/WL, EA(EA/W)L, and EA(EA/W)F and acted as antioxidant for L929. The highest apoptosis inductions were observed in Met/WL-treated HeLa and EA(EA/W)L-treated MCF-7, which were supported with the changes in mitochondrial membrane potentials. The highest caspase-9 activities were found in Met/WL-treated HeLa and EA(EA/W)F-treated MCF-7. Caspase-3 activity was only induced in EA(EA/W)F-treated HeLa.
authors.affiliation: Faculty of Science, Department of Chemistry, University of Dokuz Eylul, Izmir, Turkey. leman.tarhan@deu.edu.tr.
authors.forename: Leman
authors.initials: L
authors.lastname: Tarhan
authors.affiliation: Faculty of Science, Department of Biology, University of Dokuz Eylul, Izmir, Turkey.
authors.forename: Mahmure
authors.initials: M
authors.lastname: Nakipoğlu
authors.affiliation: Faculty of Science, Department of Chemistry, University of Dokuz Eylul, Izmir, Turkey.|The Graduate School of Natural and Applied Sciences, Izmir, Turkey.
authors.forename: Berna
authors.initials: B
authors.lastname: Kavakcıoğlu
authors.affiliation: The Graduate School of Natural and Applied Sciences, Izmir, Turkey.
authors.forename: Burcu
authors.initials: B
authors.lastname: Tongul
authors.affiliation: Faculty of Engineering, Department of Bioengineering, Ege University, Izmir, Turkey.
authors.forename: Ayşe
authors.initials: A
authors.lastname: Nalbantsoy
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D006367:HeLa Cells; D006801:Humans; D061986:MCF-7 Cells; D010936:Plant Extracts / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D031367:Teucrium / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D017382:Reactive Oxygen Species
keywords: Antioxidant; Apoptotic cell death; HeLa; Intracellular ROS levels; MCF-7; Teucrium sandrasicum