title: Orange proteomic fingerprinting: From fruit to commercial juices.
abstract: Combinatorial peptide ligand library technology, coupled to mass spectrometry, has been applied to extensively map the proteome of orange pulp and peel and, via this fingerprinting, to detect its presence in commercial orange juices and drinks. The native and denaturing extraction protocols have captured 1109 orange proteins, as identified by LC-MS/MS. This proteomic map has been searched in an orange concentrate, from a Spanish juice manufacturer, as well as in commercial orange juices and soft drinks. The presence of numerous orange proteins in commercial juices has demonstrated the genuineness of these products, prepared by using orange fruits as original ingredients. However, the low number of identified proteins in sparkling beverages has suggested that they were prepared with scarce amounts of fruit extract, thus imparting lower quality to the final products. These findings not only increase the knowledge of the orange proteome but also present a reliable analytical method to assess quality and genuineness of commercial products.
authors.affiliation: Departament de Química Analítica, Facultat de Química, Universitat de València, Dr. Moliner 50, 46100 Burjassot, Spain.
authors.forename: María Jesús
authors.initials: MJ
authors.lastname: Lerma-García
authors.affiliation: Department of Chemistry, Materials and Chemical Engineering "Giulio Natta", Polytechnic of Milan, Via Mancinelli 7, 20131 Milan, Italy.
authors.forename: Alfonsina
authors.initials: A
authors.lastname: D'Amato
authors.affiliation: Departament de Química Analítica, Facultat de Química, Universitat de València, Dr. Moliner 50, 46100 Burjassot, Spain.
authors.forename: Ernesto F
authors.initials: EF
authors.lastname: Simó-Alfonso
authors.affiliation: Department of Chemistry, Materials and Chemical Engineering "Giulio Natta", Polytechnic of Milan, Via Mancinelli 7, 20131 Milan, Italy.
authors.forename: Pier Giorgio
authors.initials: PG
authors.lastname: Righetti
authors.affiliation: Departament de Química Analítica, Facultat de Química, Universitat de València, Dr. Moliner 50, 46100 Burjassot, Spain. Electronic address: elisa.fasoli@polimi.it.
authors.forename: Elisa
authors.initials: E
authors.lastname: Fasoli
mesh_terms: D001628:Beverages / Q000032:analysis*; D032084:Citrus sinensis / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry; D020543:Proteome / Q000737:chemistry; D040901:Proteomics; D053719:Tandem Mass Spectrometry
chemical_list: D010940:Plant Proteins; D020543:Proteome
keywords: Combinatorial peptide ligand library; LC–MS/MS; Orange fruit; Orange juice; Protein; Proteomics