title: SPIM-fluid: open source light-sheet based platform for high-throughput imaging.
abstract: Light sheet fluorescence microscopy has recently emerged as the technique of choice for obtaining high quality 3D images of whole organisms/embryos with low photodamage and fast acquisition rates. Here we present an open source unified implementation based on Arduino and Micromanager, which is capable of operating Light Sheet Microscopes for automatized 3D high-throughput imaging on three-dimensional cell cultures and model organisms like zebrafish, oriented to massive drug screening.
authors.affiliation: Advance Imaging Lab, Instituto Gulbenkian de Ciência, Rua da Quinta Grande 6,Oeiras 2780-156, Portugal.
authors.forename: Emilio J
authors.initials: EJ
authors.lastname: Gualda
authors.affiliation: Advance Imaging Lab, Instituto Gulbenkian de Ciência, Rua da Quinta Grande 6,Oeiras 2780-156, Portugal ; iBET - Instituto de Biologia Experimental e Tecnológica, Oeiras 2781-901, Portugal ; Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Oeiras 2780-157, Portugal.
authors.forename: Hugo
authors.initials: H
authors.lastname: Pereira
authors.affiliation: Advance Imaging Lab, Instituto Gulbenkian de Ciência, Rua da Quinta Grande 6,Oeiras 2780-156, Portugal.
authors.forename: Tiago
authors.initials: T
authors.lastname: Vale
authors.affiliation: iBET - Instituto de Biologia Experimental e Tecnológica, Oeiras 2781-901, Portugal ; Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Oeiras 2780-157, Portugal.
authors.forename: Marta Falcão
authors.initials: MF
authors.lastname: Estrada
authors.affiliation: iBET - Instituto de Biologia Experimental e Tecnológica, Oeiras 2781-901, Portugal ; Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Oeiras 2780-157, Portugal.
authors.forename: Catarina
authors.initials: C
authors.lastname: Brito
authors.affiliation: Advance Imaging Lab, Instituto Gulbenkian de Ciência, Rua da Quinta Grande 6,Oeiras 2780-156, Portugal.
authors.forename: Nuno
authors.initials: N
authors.lastname: Moreno
keywords: (110.6880) Three-dimensional image acquisition; (180.2520) Fluorescence microscopy