title: Determination of mesalazine, a low bioavailability olsalazine metabolite in human plasma by UHPLC-MS/MS: Application to a pharmacokinetic study.
abstract: Olsalazine sodium, salicylate derivative (prodrug) is effectively bioconverted to mesalazine (5-aminosalicylic acid; 5-ASA), which has an anti-inflammatory activity in ulcerative colitis. In this article, a novel highly sensitive and selective method was developed and validated to determine mesalazine in human plasma using a derivatization technique to enhance the signal intensity by using ultra- high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS) with an electrospray ionization interface. The sample preparation consisted of a derivatization with propionyl anhydride followed by liquid liquid extraction (LLE) to remove the interference and minimize the matrix effect of human plasma. The multiple reaction monitoring (MRM) mode of the negative ion was performed and the transitions of m/z 208.1→107.0 and m/z 211.1→110.1 were used to measure the derivative of mesalazine and mesalazine-d3. The chromatographic separation was achieved using kinetex XB-C18 (100×4.6mm 2.6μ) analytical column with 0.1% formic acid in water and acetonitrile as mobile phase with a gradient elution. Nominal retention times of mesalazine and IS were 3.08 and 3.07min, respectively. Absolute recovery was found to be between 82-95% for analyte and about 78% for IS. The standard curves was linear (r(2)>0.995) in the concentration range 0.10 to 12.0ng/mL with lower limit of quantification (LLOQ) in human plasma was 0.10ng/mL. The average intra-day/inter-day precision values (%CV) were in the range from 0.6-2.9 % and 1.3-3.8 %, respectively, while the average accuracy value was 103.8-107.2%. This method has been successfully applied to the human pharmacokinetics of olsalazine sodium 250mg capsules following single oral administration.
authors.affiliation: Bioanalytical Department, QPS Bioserve India Pvt. Ltd, Balanagar, Hyderabad 500 037, India. Electronic address: banda_jagadeesh@yahoo.co.in.
authors.forename: Jagadeesh
authors.initials: J
authors.lastname: Banda
authors.affiliation: Bioanalytical Department, QPS Bioserve India Pvt. Ltd, Balanagar, Hyderabad 500 037, India.
authors.forename: Ramalingam
authors.initials: R
authors.lastname: Lakshmanan
authors.affiliation: Bioanalytical Department, QPS Bioserve India Pvt. Ltd, Balanagar, Hyderabad 500 037, India.
authors.forename: Ramesh Babu
authors.initials: RB
authors.lastname: Katepalli
authors.affiliation: Bioanalytical Department, QPS Bioserve India Pvt. Ltd, Balanagar, Hyderabad 500 037, India.
authors.forename: Uday Kumar
authors.initials: UK
authors.lastname: Reddy Venati
authors.affiliation: Bioanalytical Department, QPS Bioserve India Pvt. Ltd, Balanagar, Hyderabad 500 037, India.
authors.forename: Ramesh
authors.initials: R
authors.lastname: Koppula
authors.affiliation: Bioanalytical Department, QPS Bioserve India Pvt. Ltd, Balanagar, Hyderabad 500 037, India.
authors.forename: V V S
authors.initials: VVS
authors.lastname: Shiva Prasad
mesh_terms: D000293:Adolescent; D000328:Adult; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000097:blood* / Q000493:pharmacokinetics; D001682:Biological Availability; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D008297:Male; D019804:Mesalamine / Q000097:blood* / Q000493:pharmacokinetics; D008875:Middle Aged; D053719:Tandem Mass Spectrometry / Q000379:methods*; D055815:Young Adult
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D019804:Mesalamine
keywords: Derivatization; Human plasma; LC–MS/MS; Mesalazine; Method validation; Pharmacokinetics