title: Antioxidant Properties of Fish Protein Hydrolysates Prepared from Cod Protein Hydrolysate by Bacillus sp.
abstract: Fermentative protein hydrolysates (FPH) were prepared with a proteolytic bacterium, Bacillus strain exhibiting high proteolytic activity. Three FPH with 1, 2, and 4 % of cod protein hydrolysate (CPH) and 0.5 % of yeast extract in the culture were prepared. The yields achieved varied between 30 and 58 % based on protein content. A general decrease of leucine, isoleucine, valine, alanine, arginine, threonine, proline, and glutamic acid was observed. All FPHs showed higher reducing power and DPPH radical scavenging activity than CPH, but similar ABTS radical scavenging activity. However, FPHs exhibited lower Cu(+2)-chelating activity than CPH. The ACE inhibitory activity of FPHs was not improved relatively to that recorded in CPH. The fermentative process seems to have potential to obtaining hydrolysates with improved biological activities or even to produce protein hydrolysates from native fish proteins.
authors.affiliation: Department of Sea and Marine Resources, Division of Aquaculture and Valorization, Portuguese Institute of Sea and Atmosphere (IPMA, I.P.), Av. Brasília, 1449-006, Lisbon, Portugal.|Enviromental Chemistry Research Unit, Instituto Superior de Agronomia, University of Lisbon, Tapada da Ajuda, 1349-017, Lisbon, Portugal.
authors.forename: I
authors.initials: I
authors.lastname: Godinho
authors.affiliation: Department of Sea and Marine Resources, Division of Aquaculture and Valorization, Portuguese Institute of Sea and Atmosphere (IPMA, I.P.), Av. Brasília, 1449-006, Lisbon, Portugal. cpires@ipma.pt.|Interdisciplinary Center of Marine and Environmental Research (CIIMAR), University of Porto, Rua dos Bragas 289, 4050-123, Porto, Portugal. cpires@ipma.pt.
authors.forename: C
authors.initials: C
authors.lastname: Pires
authors.affiliation: Department of Sea and Marine Resources, Division of Aquaculture and Valorization, Portuguese Institute of Sea and Atmosphere (IPMA, I.P.), Av. Brasília, 1449-006, Lisbon, Portugal.|Interdisciplinary Center of Marine and Environmental Research (CIIMAR), University of Porto, Rua dos Bragas 289, 4050-123, Porto, Portugal.
authors.forename: S
authors.initials: S
authors.lastname: Pedro
authors.affiliation: Department of Sea and Marine Resources, Division of Aquaculture and Valorization, Portuguese Institute of Sea and Atmosphere (IPMA, I.P.), Av. Brasília, 1449-006, Lisbon, Portugal.|Interdisciplinary Center of Marine and Environmental Research (CIIMAR), University of Porto, Rua dos Bragas 289, 4050-123, Porto, Portugal.|Research Unit of Organic Chemistry, Natural and Agro-food Products (QOPNA), Chemistry Department, Aveiro University, Campus Universitário de Santiago, 3810-193, Aveiro, Portugal.
authors.forename: B
authors.initials: B
authors.lastname: Teixeira
authors.affiliation: Department of Sea and Marine Resources, Division of Aquaculture and Valorization, Portuguese Institute of Sea and Atmosphere (IPMA, I.P.), Av. Brasília, 1449-006, Lisbon, Portugal.|Interdisciplinary Center of Marine and Environmental Research (CIIMAR), University of Porto, Rua dos Bragas 289, 4050-123, Porto, Portugal.
authors.forename: R
authors.initials: R
authors.lastname: Mendes
authors.affiliation: Department of Sea and Marine Resources, Division of Aquaculture and Valorization, Portuguese Institute of Sea and Atmosphere (IPMA, I.P.), Av. Brasília, 1449-006, Lisbon, Portugal.|Interdisciplinary Center of Marine and Environmental Research (CIIMAR), University of Porto, Rua dos Bragas 289, 4050-123, Porto, Portugal.
authors.forename: M L
authors.initials: ML
authors.lastname: Nunes
authors.affiliation: Department of Sea and Marine Resources, Division of Aquaculture and Valorization, Portuguese Institute of Sea and Atmosphere (IPMA, I.P.), Av. Brasília, 1449-006, Lisbon, Portugal.|Interdisciplinary Center of Marine and Environmental Research (CIIMAR), University of Porto, Rua dos Bragas 289, 4050-123, Porto, Portugal.
authors.forename: I
authors.initials: I
authors.lastname: Batista
mesh_terms: D000596:Amino Acids / Q000737:chemistry; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001407:Bacillus / Q000378:metabolism*; D002614:Chelating Agents / Q000737:chemistry; D002850:Chromatography, Gel; D003300:Copper / Q000737:chemistry; D029941:Fish Proteins / Q000494:pharmacology*; D048389:Gadus morhua; D006868:Hydrolysis; D007501:Iron / Q000737:chemistry; D008970:Molecular Weight
chemical_list: D000596:Amino Acids; D000975:Antioxidants; D002614:Chelating Agents; D029941:Fish Proteins; D003300:Copper; D007501:Iron
keywords: Antioxidant properties; Bacillus sp.; Fermentative process; Fish protein hydrolysates