title: Cytoprotective and antioxidant effects of human lactoferrin against H2O2-induced oxidative stress in human umbilical vein endothelial cells.
abstract: BACKGROUND
  Lactoferrin (LF) is an iron-binding glycoprotein with antioxidant, anti-inflammatory and nitric oxide-dependent vasodilatory properties. In the present study, we investigated the protective and antioxidant effects of LF on H2O2-induced oxidative stress in human umbilical vein endothelial cells (HUVECs).
  
  
  METHODS
  HUVECs were pretreated by (6.25-100 μg/ml) LF for 24 h and then exposed to 0.5 mM H2O2 for 2 h. Cell viability was assessed by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The intra- and extra-cellular hydroperoxides concentration and ferric reducing antioxidant power (FRAP) were determined in pretreated cells.
  
  
  RESULTS
  Pretreatment of HUVECs with LF at the concentrations of 25-100 μg/ml significantly reduced the cytotoxicity of H2O2 in a concentration-dependent manner using MTT assay. LF pretreatment at different concentration ranges also decreased the hydroperoxides level and augmented the FRAP value in both intra-and extra-cellular assay.
  
  
  CONCLUSIONS
  These findings revealed antioxidant and cytoprotective effects of LF against H2O2-induced oxidative stress in HUVECs. With regard to the beneficial vascular activity of LF, further investigations are suggested for understanding its clinical value in human endothelial dysfunction and prevention and/or treatment of CVDs.
authors.affiliation: Department of Pharmacology and Toxicology, Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Leila
authors.initials: L
authors.lastname: Safaeian
authors.affiliation: Department of Physiology, Applied Physiology Research Center, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Shaghayegh Haghjoo
authors.initials: SH
authors.lastname: Javanmard
authors.affiliation: Department of Pharmacology and Toxicology, Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Yaser
authors.initials: Y
authors.lastname: Mollanoori
authors.affiliation: Department of Physiology, Applied Physiology Research Center, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Nasim
authors.initials: N
authors.lastname: Dana
keywords: Antioxidant; human umbilical vein endothelial cells; lactoferrin; oxidative stress