title: Short Chemical Ischemia Triggers Phosphorylation of eIF2α and Death of SH-SY5Y Cells but not Proteasome Stress and Heat Shock Protein Response in both SH-SY5Y and T98G Cells.
abstract: Both translation arrest and proteasome stress associated with accumulation of ubiquitin-conjugated protein aggregates were considered as a cause of delayed neuronal death after transient global brain ischemia; however, exact mechanisms as well as possible relationships are not fully understood. The aim of this study was to compare the effect of chemical ischemia and proteasome stress on cellular stress responses and viability of neuroblastoma SH-SY5Y and glioblastoma T98G cells. Chemical ischemia was induced by transient treatment of the cells with sodium azide in combination with 2-deoxyglucose. Proteasome stress was induced by treatment of the cells with bortezomib. Treatment of SH-SY5Y cells with sodium azide/2-deoxyglucose for 15 min was associated with cell death observed 24 h after treatment, while glioblastoma T98G cells were resistant to the same treatment. Treatment of both SH-SY5Y and T98G cells with bortezomib was associated with cell death, accumulation of ubiquitin-conjugated proteins, and increased expression of Hsp70. These typical cellular responses to proteasome stress, observed also after transient global brain ischemia, were not observed after chemical ischemia. Finally, chemical ischemia, but not proteasome stress, was in SH-SY5Y cells associated with increased phosphorylation of eIF2α, another typical cellular response triggered after transient global brain ischemia. Our results showed that short chemical ischemia of SH-SY5Y cells is not sufficient to induce both proteasome stress associated with accumulation of ubiquitin-conjugated proteins and stress response at the level of heat shock proteins despite induction of cell death and eIF2α phosphorylation.
authors.affiliation: Department of Medical Biochemistry and Division Neuroscience of Biomedical Center, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Mala Hora 4D, Martin, SK-03601, Slovak Republic.
authors.forename: Katarina
authors.initials: K
authors.lastname: Klacanova
authors.affiliation: Department of Medical Biochemistry and Division Neuroscience of Biomedical Center, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Mala Hora 4D, Martin, SK-03601, Slovak Republic.
authors.forename: Ivana
authors.initials: I
authors.lastname: Pilchova
authors.affiliation: Department of Medical Biochemistry and Division Neuroscience of Biomedical Center, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Mala Hora 4D, Martin, SK-03601, Slovak Republic.
authors.forename: Katarina
authors.initials: K
authors.lastname: Klikova
authors.affiliation: Department of Medical Biochemistry and Division Neuroscience of Biomedical Center, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Mala Hora 4D, Martin, SK-03601, Slovak Republic. racay@jfmed.uniba.sk.
authors.forename: Peter
authors.initials: P
authors.lastname: Racay
mesh_terms: D016923:Cell Death; D015687:Cell Hypoxia; D045744:Cell Line, Tumor; D059865:Endoplasmic Reticulum Stress; D015852:Eukaryotic Initiation Factor-2 / Q000378:metabolism*; D006360:Heat-Shock Proteins / Q000378:metabolism*; D006801:Humans; D009474:Neurons / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism*; D010766:Phosphorylation; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism*; D056811:Unfolded Protein Response
chemical_list: D015852:Eukaryotic Initiation Factor-2; D006360:Heat-Shock Proteins; D046988:Proteasome Endopeptidase Complex; D010100:Oxygen
keywords: Cell death; Heat shock proteins; Ischemia; Proteasome; Ubiquitin; Unfolded protein response