title: Decreased production of proinflammatory cytokines by monocytes from individuals presenting Candida-associated denture stomatitis.
abstract: Candida-associated denture stomatitis (DS) is the most frequent lesion among denture wearers, especially the elderly. DS is strongly associated with Candida albicans, as well as local and systemic factors, such as impaired immune response. Monocytes are important in the protective immune response against the fungus by the production of cytokines that recruit and activate leukocytes. There are functional changes in these cells with age, and individual alterations involving monocyte response may predispose the host to developing infections by Candida spp. In this study, our aim was to evaluate the production of TNF-α, IL-6, CXCL8, IL-1β, MCP-1 and IL-10 by monocytes from elderly denture wearers with/without DS and elderly or young non-denture wearers. We detected that monocytes from elderly denture wearers with Candida-related denture stomatitis produced lower levels of CXCL-8, IL-6 and MCP-1. This imbalance in cytokine levels was observed in spontaneous or LPS-stimulated production. Therefore, our data suggested that inherent aspects of the host, such as changes in cytokine production by monocytes, might be associated with the development and the persistence of DS irrespective of aging.
authors.affiliation: Department of Stomatology, Bauru School of Dentistry, University of São Paulo: Al. Dr. Octávio Pinheiro Brisola, 9-75, Vila Universitária, 17012-901 Bauru, SP, Brazil.
authors.forename: Karen Henriette
authors.initials: KH
authors.lastname: Pinke
authors.affiliation: Department of Stomatology, Bauru School of Dentistry, University of São Paulo: Al. Dr. Octávio Pinheiro Brisola, 9-75, Vila Universitária, 17012-901 Bauru, SP, Brazil.
authors.forename: Patrícia
authors.initials: P
authors.lastname: Freitas
authors.affiliation: Clinical Analysis Laboratory, Hospital for Rehabilitation of Craniofacial Anomalies, University of São Paulo, Bauru, SP, Brazil.
authors.forename: Narciso Almeida
authors.initials: NA
authors.lastname: Viera
authors.affiliation: Department of Pediatric Dentistry, Orthodontics and Public Health, Bauru School of Dentistry, University of São Paulo: Al. Dr. Octávio Pinheiro Brisola, 9-75, Vila Universitária, 17012-901 Bauru, SP, Brazil.
authors.forename: Heitor Marques
authors.initials: HM
authors.lastname: Honório
authors.affiliation: Department of Prosthodontics, Bauru School of Dentistry, University of São Paulo: Al. Dr. Octávio Pinheiro Brisola, 9-75, Vila Universitária, 17012-901 Bauru, SP, Brazil.
authors.forename: Vinicius Carvalho
authors.initials: VC
authors.lastname: Porto
authors.affiliation: Department of Stomatology, Bauru School of Dentistry, University of São Paulo: Al. Dr. Octávio Pinheiro Brisola, 9-75, Vila Universitária, 17012-901 Bauru, SP, Brazil. Electronic address: vanessa@fob.usp.br.
authors.forename: Vanessa Soares
authors.initials: VS
authors.lastname: Lara
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002175:Candida / Q000145:classification / Q000276:immunology* / Q000502:physiology; D002176:Candida albicans / Q000276:immunology / Q000502:physiology; D041022:Candida tropicalis / Q000276:immunology / Q000502:physiology; D002177:Candidiasis / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology; D018932:Chemokine CCL2 / Q000276:immunology / Q000378:metabolism; D016207:Cytokines / Q000276:immunology* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D054884:Host-Pathogen Interactions / Q000276:immunology; D006801:Humans; D018836:Inflammation Mediators / Q000276:immunology* / Q000378:metabolism; D015850:Interleukin-6 / Q000276:immunology / Q000378:metabolism; D016209:Interleukin-8 / Q000276:immunology / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology; D013045:Species Specificity; D013282:Stomatitis, Denture / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology
chemical_list: C111835:CCL2 protein, human; C508606:CXCL8 protein, human; D018932:Chemokine CCL2; D016207:Cytokines; D018836:Inflammation Mediators; D015850:Interleukin-6; D016209:Interleukin-8
keywords: Candida-associated denture stomatitis; Cytokines; Elderly; Monocytes