title: Correlation of long-chain fatty acid oxidation with oxidative stress and inflammation in pre-eclampsia-like mouse models.
abstract: BACKGROUND
  Pre-eclampsia has been further recognized as a syndrome during pregnancy. Recent studies have found that long-chain fatty acid oxidation (FAO) disorders may be associated with some of pre-eclampsia. However, the mechanism remains unclear. In this study, we investigated the role of FAO and its relationship with oxidative stress and inflammatory signaling pathways in the pathogenesis of pre-eclampsia.
  
  
  METHODS
  PE-like groups included ApoC3 transgenic mice with abnormal fatty acid metabolism, classical PE-like models with injection of Nω-nitro-l-arginine-methyl ester (L-NA) or lipopolysaccharide (LPS), and antiphospholipid syndrome (APS) mouse model with β2GPI injection. The control group included wild-type mice with normal saline injection. Serum FFA was compared and placental and hepatic LCHAD, p47phox and NF-κB mRNA and protein were detected using real-time quantitative PCR and western blot.
  
  
  RESULTS
  FFA levels were significantly increased and were positively correlated with P47phox and NF-κB mRNA and protein expression in liver of all groups (p < 0.05), except LPS group (p < 0.05) as compared to control. LCHAD mRNA and protein expression in the liver and placenta was significantly increased in ApoC3+NS, ApoC3+L-NA, and β2GPI group, whereas decreased in L-NA group (p < 0.05) as compared to the control group. P47phox mRNA, NF-κB mRNA, and protein expression in the liver of all groups, except in LPS and in the placenta of β2GPI and L-NA groups, significantly increased (p < 0.05).
  
  
  CONCLUSIONS
  FAO disorders were involved in the pathogenesis of pre-eclampsia through oxidative stress and inflammatory endothelial cell injury.
authors.affiliation: Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing 100191, PR China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Ding
authors.affiliation: Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing 100191, PR China. Electronic address: zi_yang2005@126.com.
authors.forename: Zi
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing 100191, PR China.
authors.forename: Yiwei
authors.initials: Y
authors.lastname: Han
authors.affiliation: Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing 100191, PR China.
authors.forename: Huan
authors.initials: H
authors.lastname: Yu
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005227:Fatty Acids / Q000378:metabolism*; D005260:Female; D007249:Inflammation / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D018384:Oxidative Stress / Q000502:physiology*; D010920:Placenta / Q000378:metabolism*; D011225:Pre-Eclampsia / Q000378:metabolism*; D011247:Pregnancy
chemical_list: D005227:Fatty Acids
keywords: Inflammation; Long-chain fatty acid; Oxidative stress; Pre-eclampsia