title: The anticancer effects of biodegradable nanomagnetic dual natural components on the leptin gene expression in lung cancer.
abstract: Lung cancer is an invasive and progressive, fatal disease with few treatment choices and poor overall survival rates in nonsurgical stages. Leptin (LEP), an adipocyte derivative cytokine, participates in carcinogenesis. Increased amounts of systemic and pulmonary LEP indicate lung cancer. Curcumin (CUR) and silibinin (SIL) are herbal compounds which have many anticancer properties, but they have hydrophobic structures and low solubility in water. In this study, evaluated CUR-SIL dual drug-loaded poly (ɛ-caprolactone) [PCL]-co-poly ethylene glycol (PEG) magnetic nanoparticles (MNPs) were used to determine the inhibitory effect on LEP gene expression. The physicochemical properties of free and CUR-SIL-loaded PCL-PEG were fully characterized. The cytotoxic effect of CUR-SIL-loaded PCL-PEG magnetic nanoparticles was determined by MTT assay. Afterward, the inhibition of LEP gene expression was specified through real-time PCR. Results indicated that CUR-SIL cytotoxicity is time- and dose-dependent. CUR-SIL loaded MNPs showed the IC50 limit in lower concentrations in comparison to net CUR-SIL. CUR-SIL loaded MNPs reduced LEP expression more than net CUR-SIL. These results revealed the possibilities of CUR-SIL-loaded MNPs as a natural and effective antitumor drug delivery system to fight lung tumors.
authors.affiliation: a Drug Applied Research Center, Tabriz University of Medical Sciences , Tabriz , Iran.|b Department of Medical Biotechnology , Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences , Tabriz , Iran.|c Aras International Branch of Tabriz University of Medical Sciences , Tabriz Iran.
authors.forename: Shivadokht
authors.initials: S
authors.lastname: Askari
authors.affiliation: a Drug Applied Research Center, Tabriz University of Medical Sciences , Tabriz , Iran.|d Department of Medical Nanotechnology , Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences , Tabriz , Iran.
authors.forename: Roya
authors.initials: R
authors.lastname: Salehi
authors.affiliation: b Department of Medical Biotechnology , Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences , Tabriz , Iran.
authors.forename: Nosratallah
authors.initials: N
authors.lastname: Zarghami
authors.affiliation: d Department of Medical Nanotechnology , Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences , Tabriz , Iran.
authors.forename: Abolfazl
authors.initials: A
authors.lastname: Akbarzadeh
authors.affiliation: a Drug Applied Research Center, Tabriz University of Medical Sciences , Tabriz , Iran.|b Department of Medical Biotechnology , Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences , Tabriz , Iran.|c Aras International Branch of Tabriz University of Medical Sciences , Tabriz Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Rahmati-Yamchi
mesh_terms: D045744:Cell Line, Tumor; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000494:pharmacology; D003474:Curcumin / Q000737:chemistry / Q000494:pharmacology; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006801:Humans; D020738:Leptin / Q000096:biosynthesis*; D008175:Lung Neoplasms / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D011091:Polyesters / Q000737:chemistry / Q000494:pharmacology; D011092:Polyethylene Glycols / Q000737:chemistry / Q000494:pharmacology; D000077385:Silybin; D012838:Silymarin / Q000737:chemistry / Q000494:pharmacology
chemical_list: D020099:Coated Materials, Biocompatible; D020738:Leptin; D058185:Magnetite Nanoparticles; D009363:Neoplasm Proteins; D011091:Polyesters; D012838:Silymarin; C016240:polycaprolactone; D011092:Polyethylene Glycols; D000077385:Silybin; D003474:Curcumin; C000595209:polyethylene glycol 1000
keywords: Curcumin; dual drug-loaded; magnetic nanoparticles; real-time PCR; silibinin