title: Isolation of Chemoresistant Cell Subpopulations.
abstract: Chemoresistance is a major challenge for cancer therapy and drives tumor relapse. The emergence, within the treated tumor mass, of specific cancer cell subpopulations endowed with high tolerance to the microenvironment stress induced by therapy is being growingly recognized as a mechanism of tumor progression. To obtain detailed information with regard to the pathways underlying survival, expansion, and microenvironmental cross talk of such chemoresistant cell subpopulations may be instrumental for cancer chemoprevention. Additionally, the obtained cell subpopulations may be used for direct screening of cancer chemopreventive compounds, in appropriate experimental settings. Here we report detailed experimental procedures that we and others have setup in order to obtain cell cultures enriched for chemoresistant cells from both malignant pleural mesothelioma specimens and primary cell cultures. We provide indications for the purification and characterization of those chemoresistant cell populations and to generally validate the obtained enriched cell populations for their chemoresistance.
authors.affiliation: Division of Thoracic Surgery, Department of Cardiothoracic Surgery, Langone Medical Center, New York University, 550 First Avenue, New York, NY, 10016, USA.
authors.forename: Claudia
authors.initials: C
authors.lastname: Canino
authors.affiliation: Division of Thoracic Surgery, Department of Cardiothoracic Surgery, Langone Medical Center, New York University, 550 First Avenue, New York, NY, 10016, USA. Mario.Cioce@nyumc.org.
authors.forename: Mario
authors.initials: M
authors.lastname: Cioce
mesh_terms: D018929:Cell Culture Techniques; D015925:Cryopreservation; D019008:Drug Resistance, Neoplasm; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology*; D008654:Mesothelioma / Q000473:pathology*; D000086002:Mesothelioma, Malignant
keywords: ALDH; Chemoresistance; Cisplatin; FACS; Mesothelioma; Pemetrexed; Primary culture