title: Influence of miR-30b regulating humoral immune response by genetic difference.
abstract: Investigation of genetic difference will be beneficial to researchers to understand the origins and nature of diseases. Previous studies have revealed that L-kynurenine (L-Kyn) level was changed significantly in patient with cancer and that miR-30b play different role in tumor cells and immune cells. Moreover, it has been also conformed that miR-30b involved in the process of L-Kyn-mediated suppression of humoral immune responses induced by lipopolysaccharide (LPS) in human normal B cells separated from volunteers' peripheral blood. Nevertheless, the miR-30b role regulating humoral immune response in B lymphoma cells has been still unclear due to the genetic difference between normal cells and tumor cells. The current study demonstrated that the selected concentration of L-Kyn (100, 1000 μM) significantly reduced the immunoglobulin M secretion induced by LPS when compared with the control group in B lymphoma, CH12.LX, and BCL-1 cells, which had, at least, incomplete dependence on Aryl hydrocarbon receptor, the receptor of L-Kyn. In addition, although L-Kyn (100 μM) significantly attenuated the expression of miR-30b in BCL-1 cells rather than in CH12.LX cells, no significant differences in the strength of L-Kyn-mediated suppression of humoral immune responses induced by LPS were detected by enzyme-linked immunosorbent assay between the LPS (10 μg/ml) + L-Kyn (100 μM) group and the LPS (10 μg/ml) + L-Kyn (100 μM) + miR-30b mimics/miR-30b inhibitor group in CH12.LX and BCL-1 cells, respectively. Further data also showed that mouse Bach2 mRNA was a novel target of miR-30b. These results suggest that genetic difference among cells has a great influence on the miR-30b role in the process of L-Kyn-mediated suppression of humoral immune responses induced by LPS.
authors.affiliation: Shanxi Medical University, 56 South Xinjian Road, Taiyuan, 030001, Shanxi, People's Republic of China. duanzhiq@163.com.|The Second Hospital of Shanxi Medical University, 382 Wuyi Road, Taiyuan, 030001, Shanxi, People's Republic of China. duanzhiq@163.com.
authors.forename: Zhi-Qing
authors.initials: ZQ
authors.lastname: Duan
authors.affiliation: Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, 935 Jiaoling Road, Kunming, 650118, Yunnan, People's Republic of China.
authors.forename: Jian-Dong
authors.initials: JD
authors.lastname: Shi
authors.affiliation: Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, 935 Jiaoling Road, Kunming, 650118, Yunnan, People's Republic of China.
authors.forename: Mei-Ni
authors.initials: MN
authors.lastname: Wu
authors.affiliation: Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, 935 Jiaoling Road, Kunming, 650118, Yunnan, People's Republic of China.
authors.forename: Ning-Zhu
authors.initials: NZ
authors.lastname: Hu
authors.affiliation: Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, 935 Jiaoling Road, Kunming, 650118, Yunnan, People's Republic of China. huyunzhangym@126.com.
authors.forename: Yun-Zhang
authors.initials: YZ
authors.lastname: Hu
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000502:physiology*; D050976:Basic-Leucine Zipper Transcription Factors / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D005786:Gene Expression Regulation; D006801:Humans; D056724:Immunity, Humoral / Q000235:genetics; D007075:Immunoglobulin M / Q000378:metabolism; D007165:Immunosuppression Therapy; D007737:Kynurenine / Q000031:analogs & derivatives / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000378:metabolism; D051379:Mice; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D011110:Polymorphism, Genetic; D018336:Receptors, Aryl Hydrocarbon / Q000378:metabolism
chemical_list: C102109:Bach2 protein, mouse; D050976:Basic-Leucine Zipper Transcription Factors; D007075:Immunoglobulin M; D008070:Lipopolysaccharides; D035683:MicroRNAs; C534715:Mirn30d microRNA, mouse; D018336:Receptors, Aryl Hydrocarbon; D007737:Kynurenine
keywords: B lymphoma cell; Genetic difference; L-Kynurenine; MiR-30b