title: Reverse Watson-Crick G-G base pair in G-quadruplex formation.
abstract: A stable intermediate dimeric G-rich form as a precursor of tetrameric G-quadruplex structures has been detected via MALDI-TOF spectrometry. Molecular dynamics simulation offered detailed insights at the atomic level, assigning reverse Watson-Crick G-G base pairing (not Hoogsteen) in the G-rich dimer. In support of this, cisplatin formed a stable adduct by binding to the dimeric G-rich structure, eliminating the possibility of G-G Hoogsteen hydrogen bond formation.
authors.affiliation: Bose Institute, Centenary Campus, Department of Biophysics, P-1/12 CIT Scheme VIIM, Kankurgachi, Kolkata-54, India. subhro_c@jcbose.ac.in.
authors.forename: Soma
authors.initials: S
authors.lastname: Mondal
authors.forename: Jyotsna
authors.initials: J
authors.lastname: Bhat
authors.forename: Jagannath
authors.initials: J
authors.lastname: Jana
authors.forename: Meghomukta
authors.initials: M
authors.lastname: Mukherjee
authors.forename: Subhrangsu
authors.initials: S
authors.lastname: Chatterjee
mesh_terms: D020029:Base Pairing; D054856:G-Quadruplexes; D006860:Hydrogen Bonding; D008958:Models, Molecular; D019906:Nuclear Magnetic Resonance, Biomolecular; D009690:Nucleic Acid Conformation; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization