title: Brevibacillus gelatinisp. nov., isolated from a hot spring.
abstract: Two Gram-stain-positive, moderately thermophilic, endospore-forming, rod-shaped, motile bacteria designated PDF4Tand PDF10, were isolated from Camkoy hot spring in the provinces of Aydın, Turkey and were characterized in order to determine their phylogenetic position. 16S rRNA gene sequence analysis revealed that the two strains belonged to the genusBrevibacillus. Strain PDF4Tshowed highest 16S rRNA gene sequence similarity to strain PDF10 (99.5 %),Brevibacillus brevisDSM 30T(98.9 %),Brevibacillus parabrevisDSM 8376T(98.6 %) andBrevibacillus formosusDSM 9885T(98.5 %); similarities to other species of the genusBrevibacilluswere less than 98.5 %. The predominant fatty acids of strain PDF4Twere anteiso-C15 : 0(60.0 %) and iso-C15 : 0(22.3 %). The polar lipids of strain PDF4Tconsisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, an unknown phospholipid, two unknown lipid, an unknown aminophospholipid and two unknown aminolipids. MK-7 was detected as a sole respiratory quinone, and the cell wall of strain PDF4Tcontainedmeso-diaminopimelic acid. The DNA G+C content of strain PDF4Twas 51.7 mol%. DNA-DNA hybridization showed less than 60 % relatedness between strain PDF4Tand type strains of the most closely related species given above. Based on these data, the two strains are considered to represent a novel species of the genusBrevibacillus, for which the nameBrevibacillus gelatinisp. nov. is proposed. The type strain is PDF4T( = NCCB 100559T= DSM 100115T).
authors.affiliation: Karadeniz Technical University Faculty of Sciences Department of Molecular Biology and Genetic, 61080 Trabzon, Turkey.
authors.forename: Kadriye
authors.initials: K
authors.lastname: Inan
authors.affiliation: Karadeniz Technical University Faculty of Sciences Department of Biology, 61080 Trabzon, Turkey.
authors.forename: Aysegul
authors.initials: A
authors.lastname: Ozer
authors.affiliation: Artvin Coruh University Faculty of Science and Art Department of Biology, 08000 Artvin, Turkey.
authors.forename: Halil
authors.initials: H
authors.lastname: Ibrahim Guler
authors.affiliation: Karadeniz Technical University Faculty of Sciences Department of Biology, 61080 Trabzon, Turkey.
authors.forename: Ali
authors.initials: A
authors.lastname: Osman Belduz
authors.affiliation: Karadeniz Technical University Faculty of Sciences Department of Biology, 61080 Trabzon, Turkey.
authors.forename: Sabriye
authors.initials: S
authors.lastname: Canakci