title: Biosensing of glucose in flow injection analysis system based on glucose oxidase-quantum dot modified pencil graphite electrode.
abstract: A novel amperometric glucose biosensor was proposed in flow injection analysis (FIA) system using glucose oxidase (GOD) and Quantum dot (ZnS-CdS) modified Pencil Graphite Electrode (PGE). After ZnS-CdS film was electrochemically deposited onto PGE surface, GOD was immobilized on the surface of ZnS-CdS/PGE through crosslinking with chitosan (CT). A pair of well-defined reversible redox peak of GOD was observed at GOD/CT/ZnS-CdS/PGE based on enzyme electrode by direct electron transfer between the protein and electrode. Further, obtained GOD/CT/ZnS-CdS/PGE offers a disposable, low cost, selective and sensitive electrochemical biosensing of glucose in FIA system based on the decrease of the electrocatalytic response of the reduced form of GOD to dissolved oxygen. Under optimum conditions (flow rate, 1.3mL min(-1); transmission tubing length, 10cm; injection volume, 100μL; and constant applied potential, -500mV vs. Ag/AgCl), the proposed method displayed a linear response to glucose in the range of 0.01-1.0mM with detection limit of 3.0µM. The results obtained from this study would provide the basis for further development of the biosensing using PGE based FIA systems.
authors.affiliation: Çanakkale Onsekiz Mart University, Faculty of Art and Science, Department of Chemistry, Çanakkale, Turkey.
authors.forename: Özlem
authors.initials: Ö
authors.lastname: Sağlam
authors.affiliation: Çanakkale Onsekiz Mart University, Faculty of Marine Sciences and Technology, Çanakkale, Turkey.
authors.forename: Bayram
authors.initials: B
authors.lastname: Kızılkaya
authors.affiliation: Çanakkale Onsekiz Mart University, Faculty of Art and Science, Department of Molecular Biology and Genetics, Çanakkale, Turkey.
authors.forename: Hüseyin
authors.initials: H
authors.lastname: Uysal
authors.affiliation: Çanakkale Onsekiz Mart University, Faculty of Art and Science, Department of Chemistry, Çanakkale, Turkey. Electronic address: ydilgin@yahoo.com.
authors.forename: Yusuf
authors.initials: Y
authors.lastname: Dilgin
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology; D015374:Biosensing Techniques / Q000191:economics / Q000295:instrumentation*; D019187:Cadmium Compounds / Q000737:chemistry; D048271:Chitosan / Q000737:chemistry; D004563:Electrochemistry; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D017022:Flow Injection Analysis; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000737:chemistry* / Q000378:metabolism; D006108:Graphite / Q000737:chemistry*; D057230:Limit of Detection; D045663:Quantum Dots / Q000737:chemistry*; D013440:Sulfides / Q000737:chemistry; D017967:Zinc Compounds / Q000737:chemistry
chemical_list: D019187:Cadmium Compounds; D004800:Enzymes, Immobilized; D013440:Sulfides; D017967:Zinc Compounds; C034939:cadmium sulfide; D006108:Graphite; D048271:Chitosan; D005949:Glucose Oxidase; D005947:Glucose; C031238:zinc sulfide
keywords: Flow injection analysis; Glucose biosensor; Glucose oxidase; Pencil graphite electrode; Quantum dots