title: Effects of antihistamine on up-regulation of histamine H1 receptor mRNA in the nasal mucosa of patients with pollinosis induced by controlled cedar pollen challenge in an environmental exposure unit.
abstract: In the present study, we examined the effects of antihistamine on the up-regulation of H1R mRNA in the nasal mucosa of patients with pollinosis induced by controlled exposure to pollen using an environmental exposure unit. Out of 20 patients, we designated 14 responders, whose levels of H1R mRNA in the nasal mucosa were increased after the first pollen exposure and excluded 6 non-responders. Accordingly, the first exposure to pollen without treatment significantly induced both nasal symptoms and the up-regulation of H1R mRNA in the nasal mucosa of the responders. Subsequently, prophylactic administration of antihistamine prior to the second pollen exposure significantly inhibited both of the above effects in the responders. Moreover, the nasal expression of H1R mRNA before the second pollen exposure in the responders pretreated with antihistamine was significantly decreased, as compared with that before the first pollen exposure without treatment. These findings suggest that antihistamines suppressed histamine-induced transcriptional activation of H1R gene in the nasal mucosa, in addition to their blocking effect against histamine on H1R, resulting in a decrease of nasal symptoms. These findings further suggest that by their inverse agonistic activity, antihistamines suppress the basal transcription of nasal H1R in the absence of histamine in responders.
authors.affiliation: Department of Otolaryngology, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, Japan. Electronic address: ykitamura@tokushima-u.ac.jp.
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Kitamura
authors.affiliation: Department of Otolaryngology, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, Japan.
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Nakagawa
authors.affiliation: Department of Otolaryngology, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, Japan.
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Fujii
authors.affiliation: Department of Otolaryngology, Rinku General Medical Center, Osaka, Japan.
authors.forename: Takema
authors.initials: T
authors.lastname: Sakoda
authors.affiliation: NPO Japan Health Promotion Supporting Network, Wakayama, Japan.
authors.forename: Tadao
authors.initials: T
authors.lastname: Enomoto
authors.affiliation: Department of Molecular Pharmacology, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Mizuguchi
authors.affiliation: Department of Molecular Studies for Incurable Diseases, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Fukui
authors.affiliation: Department of Otolaryngology, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, Japan.
authors.forename: Noriaki
authors.initials: N
authors.lastname: Takeda
mesh_terms: D000328:Adult; D000393:Air Pollutants / Q000276:immunology*; D032485:Cryptomeria / Q000276:immunology*; D004781:Environmental Exposure; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D006633:Histamine Antagonists / Q000494:pharmacology* / Q000627:therapeutic use*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009297:Nasal Mucosa / Q000276:immunology* / Q000378:metabolism*; D011058:Pollen / Q000276:immunology*; D012333:RNA, Messenger / Q000378:metabolism*; D011969:Receptors, Histamine H1 / Q000235:genetics* / Q000378:metabolism*; D006255:Rhinitis, Allergic, Seasonal / Q000188:drug therapy* / Q000276:immunology* / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects* / Q000276:immunology*
chemical_list: D000393:Air Pollutants; D006633:Histamine Antagonists; D012333:RNA, Messenger; D011969:Receptors, Histamine H1
keywords: Allergic rhinitis; Antihistamines; Cedar pollinosis; Enviromental exposure unit; Histamine H(1) receptor