title: Evaluation of Biochemical, Genetic and Hematological Biomarkers in a Commercial Catfish Rhamdia quelen Exposed to Diclofenac.
abstract: Juveniles Rhamdia quelen fish species were exposed to diclofenac for 96 h at concentrations of 0.2, 2, and 20 μg/L. Biochemical, genetic, and hematological biomarkers were assessed in the liver, kidney, and blood in order to evaluate the toxic effects. No oxidative stress was observed in liver. In kidney the superoxide dismutase activity increased in all concentrations, suggesting an alteration in the hydrogen peroxide production, but DNA damage and lipid peroxidation were not detected. Diclofenac exposure increased the red blood cells number at concentrations of 0.2 and 2 μg/L, and monocytes and neutrophils at 2 and 20 μg/L, respectively. These results suggest that acute exposure to diclofenac, even at low concentrations, caused hematologic and renal enzymatic alterations in R. quelen.
authors.affiliation: Ecology and Conservation Program of Post-Graduation, Federal University of Paraná, PO Box 19031, Curitiba, Paraná, CEP 81.531-990, Brazil.
authors.forename: Ariane
authors.initials: A
authors.lastname: Ghelfi
authors.affiliation: Department of Pharmacology, Federal University of Paraná, PO Box 19031, Curitiba, Paraná, CEP 81.531-990, Brazil.
authors.forename: João Luiz Coelho
authors.initials: JL
authors.lastname: Ribas
authors.affiliation: Department of Pharmacology, Federal University of Paraná, PO Box 19031, Curitiba, Paraná, CEP 81.531-990, Brazil.
authors.forename: Izonete Cristina
authors.initials: IC
authors.lastname: Guiloski
authors.affiliation: Department of Pharmacology, Federal University of Paraná, PO Box 19031, Curitiba, Paraná, CEP 81.531-990, Brazil.
authors.forename: Franciele Lima
authors.initials: FL
authors.lastname: Bettim
authors.affiliation: Department of Genetics, Federal University of Paraná, PO Box 19031, Curitiba, Paraná, CEP 81.531-990, Brazil.
authors.forename: Laércio Dante Stein
authors.initials: LD
authors.lastname: Piancini
authors.affiliation: Department of Genetics, Federal University of Paraná, PO Box 19031, Curitiba, Paraná, CEP 81.531-990, Brazil.
authors.forename: Marta Margarete
authors.initials: MM
authors.lastname: Cestari
authors.affiliation: Department of Biochemistry, Federal University of Paraná, PO Box 19031, Curitiba, Paraná, CEP 81.531-990, Brazil.
authors.forename: Aramis José
authors.initials: AJ
authors.lastname: Pereira
authors.affiliation: Department of Biochemistry, Federal University of Paraná, PO Box 19031, Curitiba, Paraná, CEP 81.531-990, Brazil.
authors.forename: Guilherme Lanzi
authors.initials: GL
authors.lastname: Sassaki
authors.affiliation: Department of Pharmacology, Federal University of Paraná, PO Box 19031, Curitiba, Paraná, CEP 81.531-990, Brazil. helassis@ufpr.br.
authors.forename: Helena Cristina
authors.initials: HC
authors.lastname: Silva de Assis
mesh_terms: D000818:Animals; D017756:Aquaculture; D015415:Biomarkers / Q000097:blood; D002397:Catfishes / Q000097:blood*; D004249:DNA Damage; D004008:Diclofenac / Q000633:toxicity*; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D017747:Seafood; D023382:Toxicity Tests, Acute; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D015415:Biomarkers; D014874:Water Pollutants, Chemical; D004008:Diclofenac
keywords: Biomarkers; Fish; Hematology; NSAIDs; Pharmaceuticals