title: Fecal pollution source tracking in waters intended for human supply based on archaeal and bacterial genetic markers.
abstract: The determination of fecal pollution sources in aquatic ecosystems is essential to estimate associated health risks. In this study, we evaluate eight microbial source tracking (MST) markers including host-specific Bacteroidales and Methanobrevibacter spp. for discrimination between human, bovine, equine, and swine fecal contamination in waters intended for human supply. Overall, the novel host-specific archaeal and bacterial primers proposed in this study demonstrated high sensitivity and specificity. Markers for the Archaea domain were more prevalent in the fecal and water samples studied. We conclude that the investigations regarding the sources of fecal pollution in public water supplies can contribute to improve the quality of human health. To our knowledge, this is the first analysis using both archaeal and bacterial fecal MST markers on tropical water bodies of Rio de Janeiro city, Brazil.
authors.affiliation: Instituto Nacional de Controle de Qualidade em Saúde, INCQS/FIOCRUZ, Av. Brasil, 4365 - Manguinhos, Rio de Janeiro, RJ, Brazil E-mail: maysa.mandetta@incqs.fiocruz.br.
authors.forename: Kayo
authors.initials: K
authors.lastname: Bianco
authors.affiliation: Instituto Nacional de Controle de Qualidade em Saúde, INCQS/FIOCRUZ, Av. Brasil, 4365 - Manguinhos, Rio de Janeiro, RJ, Brazil E-mail: maysa.mandetta@incqs.fiocruz.br.
authors.forename: Camila
authors.initials: C
authors.lastname: Barreto
authors.affiliation: Instituto Nacional de Controle de Qualidade em Saúde, INCQS/FIOCRUZ, Av. Brasil, 4365 - Manguinhos, Rio de Janeiro, RJ, Brazil E-mail: maysa.mandetta@incqs.fiocruz.br.
authors.forename: Samara Sant'Anna
authors.initials: SS
authors.lastname: Oliveira
authors.affiliation: Centro Biomédico do Hospital Universitário Antônio Pedro, Universidade Estadual do Rio de Janeiro, UERJ, Rua Felipe Camarão, 58 - Vila Isabel, Rio de Janeiro, RJ, Brazil.
authors.forename: Leonardo Henriques
authors.initials: LH
authors.lastname: Pinto
authors.affiliation: Centro Biomédico do Hospital Universitário Antônio Pedro, Universidade Estadual do Rio de Janeiro, UERJ, Rua Felipe Camarão, 58 - Vila Isabel, Rio de Janeiro, RJ, Brazil.
authors.forename: Rodolpho Mattos
authors.initials: RM
authors.lastname: Albano
authors.affiliation: Instituto Nacional de Controle de Qualidade em Saúde, INCQS/FIOCRUZ, Av. Brasil, 4365 - Manguinhos, Rio de Janeiro, RJ, Brazil E-mail: maysa.mandetta@incqs.fiocruz.br.
authors.forename: Catia Chaia
authors.initials: CC
authors.lastname: Miranda
authors.affiliation: Instituto Nacional de Controle de Qualidade em Saúde, INCQS/FIOCRUZ, Av. Brasil, 4365 - Manguinhos, Rio de Janeiro, RJ, Brazil E-mail: maysa.mandetta@incqs.fiocruz.br.
authors.forename: Maysa Mandetta
authors.initials: MM
authors.lastname: Clementino
mesh_terms: D000818:Animals; D000829:Animals, Domestic / Q000382:microbiology; D041963:Bacteroidetes / Q000302:isolation & purification*; D015415:Biomarkers / Q000032:analysis; D001938:Brazil; D060766:Drinking Water / Q000382:microbiology*; D005243:Feces / Q000382:microbiology*; D006801:Humans; D057230:Limit of Detection; D041581:Methanobrevibacter / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D012680:Sensitivity and Specificity
chemical_list: D015415:Biomarkers; D060766:Drinking Water; D012336:RNA, Ribosomal, 16S