title: [Suppression of Kiss-1 gene inhibits HCT116 human colorectal carcinoma cell migration in vitro via nuclear factor-κB signaling pathway].
abstract: OBJECTIVE
  To investigate the effect of Kiss-1 gene suppression on the metastatic capacity of HCT116 human colorectal carcinoma cells in vitro and the involvement of nuclear factor-κB (NF-κB) signaling pathway.
  
  
  METHODS
  A recombinant lentiviral vector of Kiss-1 gene pGC-LV-Kiss-1-EGFP or the empty vector was transfected in HCT116 cells. Cell Counting Kit-8 (CCK8) and Transwell chamber assay were used to detect the changes in cell proliferation, invasion and migration ability after the transfection. Western blotting was used to detect the expression of I-κB, the inhibitive protein of NF-κB signal pathway, and the expression of the downstream effector MMP-9 before and after transfection.
  
  
  RESULTS
  In cells over-expressing Kiss-1, I-κB expression increased and MMP-9 expression decreased significantly compared to those in the blank control and vector-transfected cells (P<0.05). Kiss-1 gene over-expression resulted in significant inhibition of HCT116 cell proliferation, invasion, and migration as compared to the control cells (P<0.05).
  
  
  CONCLUSIONS
  Lentivirus-mediated Kiss-1 gene over-expression can inhibit the proliferation, invasion, and migration of HCT116 cells via the NF-B signaling pathway.
authors.affiliation: The First Affiliated Hospital of Fujian Medical University,Fuzhou 350005, China.E-mail: chenshaoqin1613@163.com.
authors.forename: Shaoqin
authors.initials: S
authors.lastname: Chen
authors.forename: Xiaobao
authors.initials: X
authors.lastname: Su
authors.forename: Ji
authors.initials: J
authors.lastname: Gao
authors.forename: Hongjing
authors.initials: H
authors.lastname: Han
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Chen
authors.forename: Suyong
authors.initials: S
authors.lastname: Lin
mesh_terms: D002465:Cell Movement; D049109:Cell Proliferation; D015179:Colorectal Neoplasms / Q000473:pathology*; D005822:Genetic Vectors; D045325:HCT116 Cells; D006801:Humans; D051550:I-kappa B Kinase / Q000378:metabolism; D059648:Kisspeptins / Q000235:genetics*; D016086:Lentivirus; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D015398:Signal Transduction; D014162:Transfection
chemical_list: C106628:KISS1 protein, human; D059648:Kisspeptins; D016328:NF-kappa B; D051550:I-kappa B Kinase; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9