title: Developing Potential Candidates of Preclinical Preeclampsia.
abstract: The potential for developing molecules of interest in preclinical preeclampsia from candidate genes that were discovered on gene expression microarray analysis has been challenged by limited access to additional first trimester trophoblast and decidual tissues. The question of whether these candidates encode secreted proteins that may be detected in maternal circulation early in pregnancy has been investigated using various proteomic methods. Pilot studies utilizing mass spectrometry based proteomic assays, along with enzyme linked immunosorbent assays (ELISAs), and Western immunoblotting in first trimester samples are reported. The novel targeted mass spectrometry methods led to robust multiple reaction monitoring assays. Despite detection of several candidates in early gestation, challenges persist. Future antibody-based studies may lead to a novel multiplex protein panel for screening or detection to prevent or mitigate preeclampsia.
authors.affiliation: School of Nursing and Magee-Womens Research Institute, University of Pittsburgh, 3500 Victoria St. 448 VB, Pittsburgh, PA 15261, USA. foundss@pitt.edu.
authors.forename: Sandra
authors.initials: S
authors.lastname: Founds
authors.affiliation: Biomedical Mass Spectrometry Center Schools of the Health Sciences, University of Pittsburgh, Biomedical Science Tower 3, 3501 Fifth Avenue, Pittsburgh, PA 15261, USA. xuz2@pitt.edu.
authors.forename: Xuemei
authors.initials: X
authors.lastname: Zeng
authors.affiliation: Magee-Womens Research Institute, University of Pittsburgh, 204 Craft Avenue Pittsburgh, PA 15213, USA. lykinsd@upmc.edu.
authors.forename: David
authors.initials: D
authors.lastname: Lykins
authors.affiliation: School of Medicine, Graduate School of Public Health and Magee-Womens Research Institute, University of Pittsburgh, 204 Craft Avenue Pittsburgh, PA 15213, USA. jroberts@mwri.magee.edu.
authors.forename: James M
authors.initials: JM
authors.lastname: Roberts
mesh_terms: D015415:Biomarkers; D016022:Case-Control Studies; D002853:Chromatography, Liquid; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006801:Humans; D010455:Peptides / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D011225:Pre-Eclampsia / Q000235:genetics / Q000378:metabolism*; D011247:Pregnancy; D020543:Proteome; D040901:Proteomics / Q000379:methods; D053719:Tandem Mass Spectrometry
chemical_list: D015415:Biomarkers; D010455:Peptides; D020543:Proteome
keywords: biomarker development; candidate gene; candidate protein; preeclampsia; prevention