title: Widespread dispersion of the resistance element tet(B)::ISCR2 in XDR Acinetobacter baumannii isolates.
abstract: Acinetobacter baumannii is a significant nosocomial pathogen often associated with extreme drug resistance (XDR). In Argentina, isolates of A. baumannii resistant to tetracyclines have accounted for more than 40% of drug-resistant isolates in some hospitals. We have previously reported the dispersion of the tet(B) resistance element associated with the ISCR2 transposase in epidemiologically unrelated A. baumannii isolates recovered from 1983 to 2011. This study extends this surveillance to 77 recent (2009-2013) XDR A. baumannii isolates with different levels of minocycline susceptibility. Isolates were examined by a pan-PCR assay, which showed six different amplification patterns, and specific PCRs were used for the confirmation of the the ΔISCR2-tet(B)-tet(R)-ISCR2 element. The tet(B) gene was present in 66 isolates and the ISCR2 element in 68 isolates; the tet(B) gene was associated with ISCR2 in all tet(B)-positive isolates. We conclude that this element is widespread in XDR A. baumannii isolates from Argentina and could be responsible for the emergence of tetracycline resistance in recent years.
authors.affiliation: Instituto de Microbiología y Parasitología Médica (IMPaM, UBA-CONICET),Facultad de Medicina,Universidad de Buenos Aires,Argentina.
authors.forename: E
authors.identifier: 0000-0002-3528-6497
authors.initials: E
authors.lastname: Vilacoba
authors.affiliation: Laboratorio de Bacteriología, Hospital Interzonal de Agudos Eva Peron,San Martin,Buenos Aires,Argentina.
authors.forename: M
authors.initials: M
authors.lastname: Almuzara
authors.affiliation: Instituto de Microbiología y Parasitología Médica (IMPaM, UBA-CONICET),Facultad de Medicina,Universidad de Buenos Aires,Argentina.
authors.forename: L
authors.initials: L
authors.lastname: Gulone
authors.affiliation: Instituto de Microbiología y Parasitología Médica (IMPaM, UBA-CONICET),Facultad de Medicina,Universidad de Buenos Aires,Argentina.
authors.forename: G M
authors.initials: GM
authors.lastname: Traglia
authors.affiliation: Instituto de Microbiología y Parasitología Médica (IMPaM, UBA-CONICET),Facultad de Medicina,Universidad de Buenos Aires,Argentina.
authors.forename: S
authors.initials: S
authors.lastname: Montaña
authors.affiliation: Laboratorio de Bacteriología del Hospital de Clínicas,Buenos Aires,Argentina.
authors.forename: H
authors.initials: H
authors.lastname: Rodríguez
authors.affiliation: Servicio Antimicrobianos,INEI-ANLIS 'Dr. Carlos G. Malbrán',Buenos Aires,Argentina.
authors.forename: F
authors.initials: F
authors.lastname: Pasteran
authors.affiliation: Unidad Microbiología,Stamboulian,Buenos Aires,Argentina.
authors.forename: M
authors.initials: M
authors.lastname: Pennini
authors.affiliation: Unidad Microbiología,Stamboulian,Buenos Aires,Argentina.
authors.forename: A
authors.initials: A
authors.lastname: Sucari
authors.affiliation: Hospital General de Agudos 'Dr. Cosme Argerich',Buenos Aires,Argentina.
authors.forename: N
authors.initials: N
authors.lastname: Gómez
authors.affiliation: Laboratorio de Microbiología,Hospital Universitario Fundación Favaloro,Buenos Aires,Argentina.
authors.forename: A
authors.initials: A
authors.lastname: Fernández
authors.affiliation: Instituto de Microbiología y Parasitología Médica (IMPaM, UBA-CONICET),Facultad de Medicina,Universidad de Buenos Aires,Argentina.
authors.forename: D
authors.initials: D
authors.lastname: Centrón
authors.affiliation: Instituto de Microbiología y Parasitología Médica (IMPaM, UBA-CONICET),Facultad de Medicina,Universidad de Buenos Aires,Argentina.
authors.forename: M S
authors.initials: MS
authors.lastname: Ramírez
mesh_terms: D000151:Acinetobacter Infections / Q000188:drug therapy / Q000453:epidemiology* / Q000382:microbiology; D040981:Acinetobacter baumannii / Q000235:genetics* / Q000302:isolation & purification; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001118:Argentina / Q000453:epidemiology; D001426:Bacterial Proteins / Q000235:genetics; D006761:Hospitals; D006801:Humans; D008911:Minocycline / Q000494:pharmacology*; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013753:Tetracycline Resistance / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D008911:Minocycline
keywords: Antibiotic resistance; genetics; microbiology