title: Selective and Reversible Approaches Toward Imaging Redox Signaling Using Small-Molecule Probes.
abstract: CONCLUSIONS
  Recent research has identified key roles for reactive oxygen species (ROS)/reactive nitrogen species (RNS) in redox signaling, but much remains to be uncovered. Molecular imaging tools to study these processes must not only be selective to enable identification of the ROS/RNS involved but also reversible to distinguish signaling processes from oxidative stress. Fluorescent sensors offer the potential to image such processes with high spatial and temporal resolution.
  
  
  BACKGROUND
  A broad array of strategies has been developed that enable the selective sensing of ROS/RNS. More recently, attention has turned to the design of reversible small-molecule sensors of global redox state, with a further set of probes capable of reversible sensing of individual ROS/RNS.
  
  
  RESULTS
  In this study, we discuss the key challenges in achieving simultaneous detection of reversible oxidative bursts with unambiguous determination of a particular ROS/RNS.
  
  
  CONCLUSIONS
  We have highlighted key design features of small-molecule probes that show promise in enabling the study of redox signaling, identifying essential parameters that must be assessed for any new probe. Antioxid. Redox Signal. 24, 713-730.
authors.affiliation: School of Chemistry, The University of Sydney , Sydney, New South Wales, Australia .
authors.forename: Jacek L
authors.initials: JL
authors.lastname: Kolanowski
authors.affiliation: School of Chemistry, The University of Sydney , Sydney, New South Wales, Australia .
authors.forename: Amandeep
authors.initials: A
authors.lastname: Kaur
authors.affiliation: School of Chemistry, The University of Sydney , Sydney, New South Wales, Australia .
authors.forename: Elizabeth J
authors.initials: EJ
authors.lastname: New
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods; D006801:Humans; D018384:Oxidative Stress; D026361:Reactive Nitrogen Species / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D015398:Signal Transduction; D054852:Small Molecule Libraries / Q000737:chemistry* / Q000378:metabolism
chemical_list: D026361:Reactive Nitrogen Species; D017382:Reactive Oxygen Species; D054852:Small Molecule Libraries