title: The human decapping scavenger enzyme DcpS modulates microRNA turnover.
abstract: The decapping scavenger enzyme DcpS is known for its role in hydrolyzing the cap structure following mRNA degradation. Recently, we discovered a new function in miRNA degradation activation for the ortholog of DcpS in C. elegans. Here we show that human DcpS conserves its role in miRNA turnover. In human cells, DcpS is a nucleocytoplasmic shuttling protein that activates miRNA degradation independently of its scavenger decapping activity in the cytoplasmic compartment. We also demonstrate that this new function for DcpS requires the contribution of the 5'-3' exonuclease Xrn2. Our findings support a conserved role of DcpS as a modulator of miRNA turnover in animals.
authors.affiliation: St-Patrick Research Group in Basic Oncology, Centre Hospitalier Universitaire de Québec-Université Laval (Hôtel-Dieu de Québec), Laval University Cancer Research Centre, Quebec City, Québec, Canada.
authors.forename: Oussama
authors.initials: O
authors.lastname: Meziane
authors.affiliation: St-Patrick Research Group in Basic Oncology, Centre Hospitalier Universitaire de Québec-Université Laval (Hôtel-Dieu de Québec), Laval University Cancer Research Centre, Quebec City, Québec, Canada.
authors.forename: Sandra
authors.initials: S
authors.lastname: Piquet
authors.affiliation: St-Patrick Research Group in Basic Oncology, Centre Hospitalier Universitaire de Québec-Université Laval (Hôtel-Dieu de Québec), Laval University Cancer Research Centre, Quebec City, Québec, Canada.
authors.forename: Gabriel D
authors.initials: GD
authors.lastname: Bossé
authors.affiliation: Laboratoire de génomique fonctionnelle du développement embryonnaire, Centre de recherche en biologie de la reproduction, Pavillon Comtois, Faculté des sciences de l'agriculture et de l'alimentation, Université Laval, Quebec City, Québec, Canada.
authors.forename: Dominic
authors.initials: D
authors.lastname: Gagné
authors.affiliation: St-Patrick Research Group in Basic Oncology, Centre Hospitalier Universitaire de Québec-Université Laval (Hôtel-Dieu de Québec), Laval University Cancer Research Centre, Quebec City, Québec, Canada.
authors.forename: Eric
authors.initials: E
authors.lastname: Paquet
authors.affiliation: Laboratoire de génomique fonctionnelle du développement embryonnaire, Centre de recherche en biologie de la reproduction, Pavillon Comtois, Faculté des sciences de l'agriculture et de l'alimentation, Université Laval, Quebec City, Québec, Canada.
authors.forename: Claude
authors.initials: C
authors.lastname: Robert
authors.affiliation: Rare Disease Research Unit, Pfizer, 610 Main Street, Cambridge, Massachusetts, USA.
authors.forename: Michael A
authors.initials: MA
authors.lastname: Tones
authors.affiliation: St-Patrick Research Group in Basic Oncology, Centre Hospitalier Universitaire de Québec-Université Laval (Hôtel-Dieu de Québec), Laval University Cancer Research Centre, Quebec City, Québec, Canada.
authors.forename: Martin J
authors.initials: MJ
authors.lastname: Simard
mesh_terms: D021581:Active Transport, Cell Nucleus; D000595:Amino Acid Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D002467:Cell Nucleus / Q000378:metabolism*; D004722:Endoribonucleases / Q000235:genetics / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D020869:Gene Expression Profiling / Q000379:methods; D057809:HEK293 Cells; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis; D012315:RNA Caps / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid
chemical_list: D035683:MicroRNAs; D012315:RNA Caps; D004722:Endoribonucleases; C485332:DcpS protein, human