title: Production and structural elucidation of exopolysaccharide from endophytic Pestalotiopsis sp. BC55.
abstract: There is a little information on exopolysaccharide production by endophytic fungi. In this investigation endophytic Pestalotiopsis sp. BC55 was used for optimization of exopolysaccharide production. One variable at a time method and response surface methodology were adopted to find out the best culture conditions and medium compositions for maximum exopolysaccharide production. The organism produced maximum exopolysaccharide (4.320 ± 0.022 g/l EPS) in 250 ml Erlenmeyer flask containing 75 ml potato dextrose broth supplemented with (g%/l) glucose, 7.66; urea, 0.29; CaCl2, 0.05 with medium pH 6.93; after 3.76 days of incubation at 24°C. Exopolysaccharide [EPS (EP-I)] produced by this organism have Mw ∼2×10(5)Da with a melting point range of 122-124°C. Structural elucidation of the EPS (PS-I) was carried out after a series of experiments. Result indicated the presence of only (1→3)-linked β-d-glucopyranosyl moiety. The structure of the repeating unit was established as - →3)-β-d-Glcp-(1→.
authors.affiliation: Microbiology Laboratory, Department of Botany and Forestry, Vidyasagar University, Midnapore, 721 102 West Bengal, India.
authors.forename: Subhadip
authors.initials: S
authors.lastname: Mahapatra
authors.affiliation: Microbiology Laboratory, Department of Botany and Forestry, Vidyasagar University, Midnapore, 721 102 West Bengal, India. Electronic address: debu33@yahoo.com.
authors.forename: Debdulal
authors.initials: D
authors.lastname: Banerjee
mesh_terms: D001203:Ascomycota / Q000737:chemistry* / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D002845:Chromatography / Q000379:methods; D005285:Fermentation; D062610:Fungal Polysaccharides / Q000096:biosynthesis* / Q000737:chemistry* / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D008970:Molecular Weight; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D062610:Fungal Polysaccharides
keywords: Characterization; Endophytic Pestalotiopsis sp. BC55; Exopolysaccharide; Optimization; Purification