title: Efficacy of a 2% climbazole shampoo for reducing Malassezia population sizes on the skin of naturally infected dogs.
abstract: OBJECTIVE
  Shampoo therapy is often recommended for the control of Malassezia overgrowth in dogs. The aim of this study was to evaluate the in vivo activity of a 2% climbazole shampoo against Malassezia pachydermatis yeasts in naturally infected dogs.
  
  
  METHODS
  Eleven research colony Beagles were used.
  
  
  METHODS
  The dogs were distributed randomly into two groups: group A (n=6) and group B (n=5). Group A dogs were washed with a 2% climbazole shampoo, while group B dogs were treated with a physiological shampoo base. The shampoos were applied once weekly for two weeks. The population size of Malassezia yeasts on skin was determined by fungal culture through modified Dixon's medium contact plates pressed on left concave pinna, axillae, groins, perianal area before and after shampoo application. Samples collected were compared by Wilcoxon rank sum test.
  
  
  RESULTS
  Samples collected after 2% climbazole shampoo application showed a significant and rapid reduction of Malassezia population sizes. One hour after the first climbazole shampoo application, Malassezia reduction was already statistically significant and 15 days after the second climbazole shampoo, Malassezia population sizes were still significantly decreased. No significant reduction of Malassezia population sizes was observed in group B dogs.
  
  
  CONCLUSIONS
  The application of a 2% climbazole shampoo significantly reduced Malassezia population sizes on the skin of naturally infected dogs. Application of 2% climbazole shampoo may be useful for the control of Malassezia overgrowth and it may be also proposed as prevention when recurrences are frequent.
authors.affiliation: Department of Parasitology, Mycology and Dermatology, école nationale vétérinaire d'Alfort, UPE, 94704 Maisons-Alfort cedex, France. Electronic address: paolacvet@libero.it.
authors.forename: P
authors.initials: P
authors.lastname: Cavana
authors.affiliation: Dynamyc research group, école nationale vétérinaire d'Alfort, UPEC, 94704 Maisons-Alfort cedex, France; Institut de recherche clinique d'Alfort, école nationale vétérinaire d'Alfort, UPE, 94704 Maisons-Alfort, France.
authors.forename: J-Y
authors.initials: JY
authors.lastname: Petit
authors.affiliation: Institut de recherche clinique d'Alfort, école nationale vétérinaire d'Alfort, UPE, 94704 Maisons-Alfort, France.
authors.forename: S
authors.initials: S
authors.lastname: Perrot
authors.affiliation: Department of Parasitology, Mycology and Dermatology, école nationale vétérinaire d'Alfort, UPE, 94704 Maisons-Alfort cedex, France.
authors.forename: R
authors.initials: R
authors.lastname: Guechi
authors.affiliation: Department of Parasitology, Mycology and Dermatology, école nationale vétérinaire d'Alfort, UPE, 94704 Maisons-Alfort cedex, France.
authors.forename: G
authors.initials: G
authors.lastname: Marignac
authors.affiliation: Inra, école nationale vétérinaire d'Alfort, UMR1198 biologie du développement et reproduction, 94704 Maisons-Alfort cedex, France.
authors.forename: K
authors.initials: K
authors.lastname: Reynaud
authors.affiliation: Department of Parasitology, Mycology and Dermatology, école nationale vétérinaire d'Alfort, UPE, 94704 Maisons-Alfort cedex, France; Dynamyc research group, école nationale vétérinaire d'Alfort, UPEC, 94704 Maisons-Alfort cedex, France.
authors.forename: J
authors.initials: J
authors.lastname: Guillot
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D000935:Antifungal Agents / Q000008:administration & dosage*; D015169:Colony Count, Microbial; D003881:Dermatomycoses / Q000188:drug therapy* / Q000662:veterinary; D004283:Dog Diseases / Q000188:drug therapy*; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D007093:Imidazoles / Q000008:administration & dosage*; D008290:Malassezia / Q000187:drug effects* / Q000254:growth & development; D008297:Male; D012867:Skin / Q000187:drug effects / Q000382:microbiology; D016896:Treatment Outcome
chemical_list: D000935:Antifungal Agents; D007093:Imidazoles; C108286:climbazole
keywords: Chien; Climbazole; Dog; In vivo; Malassezia pachydermatis; Shampoo; Shampooing