title: All-plastic, miniature, digital fluorescence microscope for three part white blood cell differential measurements at the point of care.
abstract: Three-part differential white blood cell counts are used for disease diagnosis and monitoring at the point-of-care. A low-cost, miniature achromatic microscope was fabricated for identification of lymphocytes, monocytes, and granulocytes in samples of whole blood stained with acridine orange. The microscope was manufactured using rapid prototyping techniques of diamond turning and 3D printing and is intended for use at the point-of-care in low-resource settings. The custom-designed microscope requires no manual adjustment between samples and was successfully able to classify three white blood cell types (lymphocytes, granulocytes, and monocytes) using samples of peripheral whole blood stained with acridine orange.
authors.affiliation: Department of Bioengineering, Rice University, 6500 Main Street, Houston, TX 77030, USA.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Forcucci
authors.affiliation: Department of Bioengineering, Rice University, 6500 Main Street, Houston, TX 77030, USA.
authors.forename: Michal E
authors.initials: ME
authors.lastname: Pawlowski
authors.affiliation: Department of Bioengineering, Rice University, 6500 Main Street, Houston, TX 77030, USA.
authors.forename: Catherine
authors.initials: C
authors.lastname: Majors
authors.affiliation: Department of Bioengineering, Rice University, 6500 Main Street, Houston, TX 77030, USA.
authors.forename: Rebecca
authors.initials: R
authors.lastname: Richards-Kortum
authors.affiliation: Department of Bioengineering, Rice University, 6500 Main Street, Houston, TX 77030, USA.
authors.forename: Tomasz S
authors.initials: TS
authors.lastname: Tkaczyk
keywords: (170.1470) Blood or tissue constituent monitoring; (170.2520) Fluorescence microscopy; (170.3880) Medical and biological imaging