title: Cold Temperature Induces the Reprogramming of Proteolytic Pathways in Yeast.
abstract: Despite much evidence of the involvement of the proteasome-ubiquitin signaling system in temperature stress response, the dynamics of the ubiquitylome during cold response has not yet been studied. Here, we have compared quantitative ubiquitylomes from a strain deficient in proteasome substrate recruitment and a reference strain during cold response. We have observed that a large group of proteins showing increased ubiquitylation in the proteasome mutant at low temperature is comprised by reverses suppressor of Ty-phenotype 5 (Rsp5)-regulated plasma membrane proteins. Analysis of internalization and degradation of plasma membrane proteins at low temperature showed that the proteasome becomes determinant for this process, whereas, at 30 °C, the proteasome is dispensable. Moreover, our observations indicate that proteasomes have increased capacity to interact with lysine 63-polyubiquitylated proteins during low temperature in vivo. These unanticipated observations indicate that, during cold response, there is a proteolytic cellular reprogramming in which the proteasome acquires a role in the endocytic-vacuolar pathway.
authors.affiliation: From the Institut de Biologia Molecular de Barcelona, Consejo Superior de Investigaciones Científicas, Barcelona Science Park, Baldiri i Reixac 15-21, 08028 Barcelona, Spain and; Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115.
authors.forename: Marta
authors.initials: M
authors.lastname: Isasa
authors.affiliation: From the Institut de Biologia Molecular de Barcelona, Consejo Superior de Investigaciones Científicas, Barcelona Science Park, Baldiri i Reixac 15-21, 08028 Barcelona, Spain and.
authors.forename: Clara
authors.initials: C
authors.lastname: Suñer
authors.affiliation: From the Institut de Biologia Molecular de Barcelona, Consejo Superior de Investigaciones Científicas, Barcelona Science Park, Baldiri i Reixac 15-21, 08028 Barcelona, Spain and.
authors.forename: Miguel
authors.initials: M
authors.lastname: Díaz
authors.affiliation: From the Institut de Biologia Molecular de Barcelona, Consejo Superior de Investigaciones Científicas, Barcelona Science Park, Baldiri i Reixac 15-21, 08028 Barcelona, Spain and.
authors.forename: Pilar
authors.initials: P
authors.lastname: Puig-Sàrries
authors.affiliation: From the Institut de Biologia Molecular de Barcelona, Consejo Superior de Investigaciones Científicas, Barcelona Science Park, Baldiri i Reixac 15-21, 08028 Barcelona, Spain and.
authors.forename: Alice
authors.initials: A
authors.lastname: Zuin
authors.affiliation: From the Institut de Biologia Molecular de Barcelona, Consejo Superior de Investigaciones Científicas, Barcelona Science Park, Baldiri i Reixac 15-21, 08028 Barcelona, Spain and.
authors.forename: Anne
authors.initials: A
authors.lastname: Bichman
authors.affiliation: Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115.
authors.forename: Steven P
authors.initials: SP
authors.lastname: Gygi
authors.affiliation: From the Institut de Biologia Molecular de Barcelona, Consejo Superior de Investigaciones Científicas, Barcelona Science Park, Baldiri i Reixac 15-21, 08028 Barcelona, Spain and.
authors.forename: Elena
authors.initials: E
authors.lastname: Rebollo
authors.affiliation: From the Institut de Biologia Molecular de Barcelona, Consejo Superior de Investigaciones Científicas, Barcelona Science Park, Baldiri i Reixac 15-21, 08028 Barcelona, Spain and. Electronic address: bernat.crosas@ibmb.csic.es.
authors.forename: Bernat
authors.initials: B
authors.lastname: Crosas
mesh_terms: D003080:Cold Temperature; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D046988:Proteasome Endopeptidase Complex / Q000235:genetics / Q000378:metabolism; D059748:Proteolysis; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D025801:Ubiquitin / Q000378:metabolism
chemical_list: D008565:Membrane Proteins; D029701:Saccharomyces cerevisiae Proteins; D025801:Ubiquitin; D046988:Proteasome Endopeptidase Complex
keywords: Can1; Itr1; Rad23; Rpn10; endocytosis; low temperature; proteasome; protein degradation; ubiquitin; vacuole