title: Adaptation of Volvariella volvacea metabolism in high carbon to nitrogen ratio media.
abstract: Increasing glucose concentration (up to 46%) in the culture medium of Volvariella volvacea resulted in a noticeable biomass increase (63.82%) and intracellular polysaccharide (IPS) production (32.84% in absolute and 51.34% in relative values). In high glucose concentrations, decreased biomass yields on glucose consumed were recorded. A non-negligible lipid was synthesized (up to 32.22%, w/w) in the first growth steps. Lipids and IPS were decreased as fermentation proceeded. Fatty acid composition of lipids was not remarkably affected by the C/N ratio imposed, whereas the concentration of neutral lipids increased in the high C/N ratio media. Phospholipid was the richest in saturated fatty acids lipid fraction. The major phospholipid classes, i.e. phosphatidyl-choline and phosphatidyl-inositol, were differentially affected by the high glucose concentrations, as the former increased its proportion and the latter decreased. Linoleic was the predominate fatty acid. C/N ratio and fermentation time affected IPS composition, as glucose increased in a high C/N ratio, while fructose and mannitol decreased.
authors.affiliation: Laboratory of Edible Fungi, Institute of Technology of Agricultural Products (ITAP), Hellenic Agricultural Organization - Demeter, 1 Sofokli Venizelou Street, 14123 - Lykovryssi, Attiki, Greece.
authors.forename: Panagiota
authors.initials: P
authors.lastname: Diamantopoulou
authors.affiliation: Department of Food Science and Technology, Agricultural University of Athens, 75 Iera Odos, 11855 Athens, Greece.
authors.forename: Seraphim
authors.initials: S
authors.lastname: Papanikolaou
authors.affiliation: Unit of Microbiology, Department of Biology, Division of Genetics, Cell Biology and Development, University of Patras, 26500 Patras, Greece.
authors.forename: George
authors.initials: G
authors.lastname: Aggelis
authors.affiliation: Laboratory of Edible Fungi, Institute of Technology of Agricultural Products (ITAP), Hellenic Agricultural Organization - Demeter, 1 Sofokli Venizelou Street, 14123 - Lykovryssi, Attiki, Greece. Electronic address: aphilippoussis@nagref.gr.
authors.forename: Antonios
authors.initials: A
authors.lastname: Philippoussis
mesh_terms: D018533:Biomass; D002244:Carbon / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism*; D010743:Phospholipids; D011134:Polysaccharides; D055367:Volvariella / Q000378:metabolism*
chemical_list: D010743:Phospholipids; D011134:Polysaccharides; D002244:Carbon; D005947:Glucose; D009584:Nitrogen
keywords: Biomass; Fungi; Glucose; Lipids; Polysaccharides