title: Effect of Endometrial Injury on Secretion of Endometrial Cytokines and IVF Outcomes in Women with Unexplained Subfertility.
abstract: In order to determine the effect of endometrial injury (EI) on in vitro fertilization (IVF) outcomes in women with unexplained subfertility and explore the relationship between EI and endometrial inflammatory cytokines, 66 women with unexplained subfertility undergoing IVF treatment were recruited. 38 patients in the EI group underwent EI in the mid-luteal phase of the cycle and 28 patients in the non-EI (NEI) group. According to the pregnancy outcome, the NEI and EI groups were divided into NEI-nonpregnant (NEI-NP), NEI-pregnant (NEI-P), EI-NP, and EI-P. All patients underwent aspiration of endometrial secretions immediately before embryo transfer. The concentrations of ten mediators were measured using Milliplex Magnetic Bead assay. The clinical pregnancy was significantly higher in the EI than in the NEI group. The concentrations of interleukin- (IL-) 6, IL-8, IL-12 (p70), IL-13, interferon- (IFN-) γ, monocyte chemotactic protein- (MCP-) 1, and vascular endothelial growth factor (VEGF) were significantly higher in the EI than the NEI group. The expression of IFN-γ and VEGF in the EI-P was significantly increased compared to the EI-NP group. These findings suggest that, in women with unexplained subfertility, endometrial injury might be a potential method to improve clinical pregnancy rates by promoting the expression of IFN-γ and VEGF.
authors.affiliation: Assisted Reproduction Center of Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100026, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Liang
authors.affiliation: Institute of Infectious Diseases, Beijing Ditan Hospital, Capital Medical University, Beijing 100011, China ; Beijing Key Laboratory of Emerging Infectious Diseases, Beijing 100011, China.
authors.forename: Junyan
authors.initials: J
authors.lastname: Han
authors.affiliation: Assisted Reproduction Center of Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100026, China.
authors.forename: Chanwei
authors.initials: C
authors.lastname: Jia
authors.affiliation: Assisted Reproduction Center of Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100026, China.
authors.forename: Yanmin
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Assisted Reproduction Center of Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100026, China.
authors.forename: Yonglian
authors.initials: Y
authors.lastname: Lan
authors.affiliation: Assisted Reproduction Center of Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100026, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.affiliation: Assisted Reproduction Center of Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100026, China.
authors.forename: Shuyu
authors.initials: S
authors.lastname: Wang
mesh_terms: D000328:Adult; D016207:Cytokines / Q000378:metabolism*; D004717:Endometrium / Q000276:immunology / Q000293:injuries* / Q000378:metabolism; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D007246:Infertility / Q000276:immunology*; D007371:Interferon-gamma / Q000378:metabolism; D011247:Pregnancy; D011446:Prospective Studies; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D016207:Cytokines; D042461:Vascular Endothelial Growth Factor A; D007371:Interferon-gamma