title: Detection of the Phosphorylation of the Estrogen Receptor α as an Outcome of GPR30 Activation.
abstract: Phosphorylation of the serine residues in estrogen receptor (ER) α is important in transcriptional activation. Hence, methods to detect such posttranslational modification events are valuable. We describe, in detail, the analysis of the phosphorylated ERα by electrophoretic separation of proteins and subsequent immuno-blotting techniques. In particular, phosphorylation of the ERα is one possible outcome of activation of the putative membrane estrogen receptor (mER), GPR30. Hence, phosphorylation represents a cross talk event between GPR30 and ERα and may be important in estrogen-regulated physiology.
authors.affiliation: Department of Cell and Molecular Biology, Tulane University, 2000 Percival Stern Hall, New Orleans, LA, 70118, USA.
authors.forename: Sara
authors.initials: S
authors.lastname: Clark
authors.affiliation: The Neuroscience Program, Tulane University, New Orleans, LA, 70118, USA.
authors.forename: Kevin
authors.initials: K
authors.lastname: Pollard
authors.affiliation: Department of Cell and Molecular Biology, Tulane University, 2000 Percival Stern Hall, New Orleans, LA, 70118, USA.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Rainville
authors.affiliation: Department of Cell and Molecular Biology, Tulane University, 2000 Percival Stern Hall, New Orleans, LA, 70118, USA. nandini@tulane.edu.|The Neuroscience Program, Tulane University, New Orleans, LA, 70118, USA. nandini@tulane.edu.
authors.forename: Nandini
authors.initials: N
authors.lastname: Vasudevan
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D003517:Cyclopentanes / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D004958:Estradiol / Q000494:pharmacology; D047628:Estrogen Receptor alpha / Q000819:agonists / Q000378:metabolism*; D005260:Female; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D051379:Mice; D009919:Orchiectomy; D010052:Ovariectomy; D010766:Phosphorylation; D011804:Quinolines / Q000494:pharmacology; D020239:Receptor Cross-Talk; D011960:Receptors, Estrogen / Q000378:metabolism*; D043562:Receptors, G-Protein-Coupled / Q000819:agonists / Q000378:metabolism*; D012694:Serine; D015398:Signal Transduction / Q000187:drug effects; D057188:Workflow
chemical_list: C517943:1-(4-(6-bromobenzo(1,3)dioxol-5-yl)-3a,4,5,9b-tetrahydro-3H-cyclopenta(c)quinolin-8-yl)ethanone; D003517:Cyclopentanes; D047628:Estrogen Receptor alpha; C532507:GPER1 protein, mouse; D011804:Quinolines; D011960:Receptors, Estrogen; D043562:Receptors, G-Protein-Coupled; D012694:Serine; D004958:Estradiol
keywords: Antibody; Cell line; ERα; GPR30; Lysate; Nuclear receptor; Phosphorylation; Serine; Tissue