title: Changes in Non-Enzymatic Antioxidants in the Blood Following Anaerobic Exercise in Men and Women.
abstract: OBJECTIVE
  The aim of this study was to compare changes in total oxidative status (TOS), total antioxidative capacity (TAC) and the concentration of VitA, VitE, VitC, uric acid (UA), reduced (GSH) and oxidized glutathione (GSSG) in blood within 24 hours following anaerobic exercise (AnEx) among men and women.
  
  
  METHODS
  10 women and 10 men performed a 20-second bicycle sprint (AnEx). Concentrations of oxidative stress indicators were measured before AnEx and 3, 15 and 30 minutes and 1 hour afterwards. UA, GSH and GSSH were also measured 24 hours after AnEx. Lactate and H+ concentrations were measured before and 3 minutes after AnEx.
  
  
  RESULTS
  The increase in lactate and H+ concentrations following AnEx was similar in both sexes. Changes in the concentrations of all oxidative stress indicators were significant and did not differ between men and women. In both sexes, TOS, TAC, TOS/TAC and VitA and VitE concentrations were the highest 3 minutes, VitC concentration was the highest 30 minutes, and UA concentration was the highest 1 hour after AnEx. GSH concentration was significantly lower than the initial concentration from 15 minutes to 24 hour after AnEx. GSSG concentration was significantly higher, while the GSH/GSSG ratio was significantly lower than the initial values 1 hour and 24 hour after AnEx.
  
  
  CONCLUSIONS
  With similar changes in lactate and H+ concentrations, AnEx induces the same changes in TAC, TOS, TOS/TAC and non-enzymatic antioxidants of low molecular weight in men and women. Oxidative stress lasted at least 24 hours after AnEx.
authors.affiliation: Department of Physiology and Biochemistry, Faculty of Physical Education and Sport, University of Physical Education in Krakow, Krakow, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Wiecek
authors.affiliation: Department of Physiology and Biochemistry, Faculty of Physical Education and Sport, University of Physical Education in Krakow, Krakow, Poland.
authors.forename: Marcin
authors.initials: M
authors.lastname: Maciejczyk
authors.affiliation: Department of Clinical Rehabilitation, Faculty of Motor Rehabilitation, University of Physical Education in Krakow, Krakow, Poland.
authors.forename: Jadwiga
authors.initials: J
authors.lastname: Szymura
authors.affiliation: Department of Sports Medicine and Human Nutrition, Faculty of Physical Education and Sport, University of Physical Education in Krakow, Krakow, Poland.
authors.forename: Zbigniew
authors.initials: Z
authors.lastname: Szygula
authors.affiliation: Faculty of Physical Education and Sport, University of Physical Education in Krakow, Krakow, Poland.
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Kantorowicz
mesh_terms: D000328:Adult; D000693:Anaerobiosis; D000886:Anthropometry; D000975:Antioxidants / Q000378:metabolism*; D001205:Ascorbic Acid / Q000097:blood; D015415:Biomarkers / Q000097:blood*; D015444:Exercise / Q000502:physiology*; D005260:Female; D005978:Glutathione / Q000097:blood; D019803:Glutathione Disulfide / Q000097:blood; D006801:Humans; D008297:Male; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D012737:Sex Factors; D013997:Time Factors; D014527:Uric Acid / Q000097:blood; D014801:Vitamin A / Q000097:blood; D014810:Vitamin E / Q000097:blood; D055815:Young Adult
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D014801:Vitamin A; D014810:Vitamin E; D014527:Uric Acid; D005978:Glutathione; D001205:Ascorbic Acid; D019803:Glutathione Disulfide