title: Structure characterization of phospholipids and lipid A of Pseudomonas putida KT2442.
abstract: Pseudomonas putida KT2442 is an important bacterium for producing various types of polyhydroxyalkanoate polymers. Phospholipids and lipid A in membranes of P. putida play important roles in stress responses, but detailed structural information of these lipids is not known. In this study, phospholipids and lipid A were isolated from P. putida KT2442, and their structures were analyzed using thin layer chromatography, high performance liquid chromatography, and electrospray ionization/mass spectrometry. Major phospholipids in P. putida KT2442 were phosphatidylethanolamine (79.9%), phosphatidylglycero1 (12.7%), and cardiolipin (7.4%), with C16:1 and/or C18:1 acyl chains. Four lipid A species were found in P. putida KT2442: two are hexa-acylated, and the other two are penta-acylated. Compared with lipid A of P. aeruginosa, P. putida lipid A has less hydroxylation on the secondary acyl chains and less modification. Therefore, P. putida lipid A could be used as a base structure to investigate lipid A modification of P. aeruginosa for understanding its pathogenesis.
authors.affiliation: State Key Laboratory of Food Science and Technology, and School of Biotechnology, Jiangnan University, 1800 Lihu Avenue, Wuxi 21422, China. yugianw@126.com.
authors.forename: Yuqian
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Food Science and Technology, and School of Biotechnology, Jiangnan University, 1800 Lihu Avenue, Wuxi 21422, Chinaof Biotechnology, State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi 21422, China. jndxwjl@126.com.
authors.forename: Jianli
authors.initials: J
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Food Science and Technology, and School of Biotechnology, Jiangnan University, 1800 Lihu Avenue, Wuxi 21422, Chinaotechnology, State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi 21422, China. liye386@sina.com.
authors.forename: Ye
authors.initials: Y
authors.lastname: Li
authors.affiliation: State Key Laboratory of Food Science and Technology, and School of Biotechnology, Jiangnan University, 1800 Lihu Avenue, Wuxi 21422, Chinaotechnology, State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi 21422, China. biwenwang@hotmail.com.
authors.forename: Biwen
authors.initials: B
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Food Science and Technology, and School of Biotechnology, Jiangnan University, 1800 Lihu Avenue, Wuxi 21422, Chinaotechnology, State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi 21422, China. taogj@sina.com.
authors.forename: Guanjun
authors.initials: G
authors.lastname: Tao
authors.affiliation: State Key Laboratory of Food Science and Technology, and School of Biotechnology, Jiangnan University, 1800 Lihu Avenue, Wuxi 21422, Chinaechnology, State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi 21422, China. xiaoyuanwang@hotmail.com.
authors.forename: Xiaoyuan
authors.initials: X
authors.lastname: Wang
mesh_terms: D000643:Ammonium Chloride / Q000737:chemistry; D002308:Cardiolipins / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D006868:Hydrolysis; D006900:Hydroxylation; D008050:Lipid A / Q000737:chemistry*; D015394:Molecular Structure; D010714:Phosphatidylethanolamines / Q000032:analysis; D010743:Phospholipids / Q000032:analysis / Q000737:chemistry*; D016958:Pseudomonas putida / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D002308:Cardiolipins; D008050:Lipid A; D010714:Phosphatidylethanolamines; D010743:Phospholipids; D000643:Ammonium Chloride; C483858:phosphatidylethanolamine