title: Biogenic selenium nanoparticles inhibit Staphylococcus aureus adherence on different surfaces.
abstract: The global issue of nosocomial infection is owing to bacterial colonization and biofilm formation on medical devices which primarily affects critically ill and/or immuno-compromised patients and also leads to malfunctioning of the devices. Therefore, it is desirable to prevent bacterial colonization on these devices by coating with a non toxic antimicrobial agent or bacterial adherence inhibitor. Here we have shown Bacillus licheniformis JS2 derived selenium nanoparticles (SeNPs) inhibit Staphylococcus aureus adherence and micro-colony formation on polystyrene, glass, and catheter surface. Results indicated that, the coating of these non toxic biogenic SeNPs, at a concentration of 0.5 mgSe/ml, prohibits bacterial load to more than 60% on glass and catheter surface, when incubated at 4 °C for 24h in phosphate buffered saline. Furthermore, confocal and electron microscopic observations strongly suggested the inhibition of biofilm and micro-colony formation on SeNP coated glass and catheter surfaces when cultured at 37 °C for 72 h in a nutrient rich medium. The study suggests that coating of biogenic SeNPs on medical devices could be an alternative approach for prevention of biofilm related infections.
authors.affiliation: Institute of Microbial Technology, Sector 39 A, Chandigarh 160036, India.
authors.forename: Praveen
authors.initials: P
authors.lastname: Sonkusre
authors.affiliation: Institute of Microbial Technology, Sector 39 A, Chandigarh 160036, India. Electronic address: ssc@imtech.res.in.
authors.forename: Swaranjit
authors.initials: S
authors.lastname: Singh Cameotra
mesh_terms: D001422:Bacterial Adhesion / Q000187:drug effects*; D003470:Culture Media; D053768:Metal Nanoparticles; D018613:Microscopy, Confocal; D012643:Selenium / Q000737:chemistry*; D013211:Staphylococcus aureus / Q000166:cytology / Q000187:drug effects*; D013499:Surface Properties
chemical_list: D003470:Culture Media; D012643:Selenium
keywords: Adherence; Bacillus licheniformis JS2; Biofilm formation; Micro-colony; Selenium nanoparticles; Staphylococcus aureus