title: Acclimation of a marine microbial consortium for efficient Mn(II) oxidation and manganese containing particle production.
abstract: Sediment contamination with metals is a widespread concern in the marine environment. Manganese oxidizing bacteria (MOB) are extensively distributed in various environments, but a marine microbial community containing MOB is rarely reported. In this study, a consortium of marine metal-contaminated sediments was acclimated using Mn(II). The shift in community structure was determined through high-throughput sequencing. In addition, the consortium resisted several harsh conditions, such as toxic metals (1mM Cu(II) and Fe(III)), and exhibited high Mn(II) oxidation capacities even the Mn(II) concentration was up to 5mM. Meanwhile, biogenic Mn containing particles were characterized by scanning electron microscope (SEM), X-ray powder diffraction (XRD), and N2 adsorption/desorption. Dye removal performance of the Mn containing particles was assayed using methylene blue, and 20.8 mg g(-1) adsorption capacity was obtained. Overall, this study revealed several new genera associated with Mn(II) oxidation and rare biogenic Na3MnPO4CO3. Results suggested the complexity of natural microbe-mediated Mn transformation.
authors.affiliation: Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), School of Food and Environment, Dalian University of Technology, Panjin 124221, China. Electronic address: zhouhao@dlut.edu.cn.
authors.forename: Hao
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), School of Food and Environment, Dalian University of Technology, Panjin 124221, China.
authors.forename: Haixia
authors.initials: H
authors.lastname: Pan
authors.affiliation: School of Life Science and Medicine, Dalian University of Technology, Panjin 124221, China.
authors.forename: Jianqiang
authors.initials: J
authors.lastname: Xu
authors.affiliation: Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), School of Food and Environment, Dalian University of Technology, Panjin 124221, China.
authors.forename: Weiping
authors.initials: W
authors.lastname: Xu
authors.affiliation: Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), School of Food and Environment, Dalian University of Technology, Panjin 124221, China.
authors.forename: Lifen
authors.initials: L
authors.lastname: Liu
mesh_terms: D000327:Adsorption; D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D004396:Coloring Agents / Q000737:chemistry; D004269:DNA, Bacterial / Q000032:analysis; D019015:Geologic Sediments / Q000382:microbiology*; D008345:Manganese / Q000737:chemistry / Q000378:metabolism*; D053768:Metal Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D008751:Methylene Blue / Q000737:chemistry; D059013:Microbial Consortia; D008855:Microscopy, Electron, Scanning; D010084:Oxidation-Reduction; D012623:Seawater / Q000382:microbiology
chemical_list: D004396:Coloring Agents; D004269:DNA, Bacterial; D008345:Manganese; D008751:Methylene Blue
keywords: Bioremediation; High-throughput sequencing; Manganese oxidizing bacteria; Microbial consortium