title: High-fat Diet-induced Intestinal Hyperpermeability is Associated with Increased Bile Acids in the Large Intestine of Mice.
abstract: Metabolic syndrome is characterized by low-grade chronic systemic inflammation, which is associated with intestinal hyperpermeability. This study examined the effects of 3 high-fat diets (HFDs) composed of different fat sources (soybean oil and lard) on the intestinal permeability, tight junction (TJ) protein expression, and cecal bile acid (BA) concentrations in mice, and then analyzed their interrelations. C57/BL6 mice were fed the control diet, HFD (soybean oil), HFD (lard), and HFD (mix; containing equal concentrations of soybean oil and lard) for 8 wk. Glucose tolerance, intestinal permeability, TJ protein expression, and cecal BA concentration were evaluated. Feeding with the 3 HDFs similarly increased body weight, liver weight, and fat pad weight, and induced glucose intolerance and intestinal hyperpermeability. The expression of TJ proteins, zonula occludens-2 and junctional adhesion molecule-A, were lower in the colons of the 3 HFD groups than in the control group (P < 0.05), and these changes appeared to be related to intestinal hyperpermeability. Feeding with HFDs increased total secondary BA (SBA) and total BA concentrations along with increases in some individual BAs in the cecum. Significant positive correlations between intestinal permeability and the concentrations of most SBAs, such as deoxycholic acid and ω-muricholic acids, were detected (P < 0.05). These results suggest that the HFD-induced intestinal hyperpermeability is associated with increased BA secretion. The abundance of SBAs in the large intestine may be responsible for the hyperpermeability.
authors.affiliation: Dept. of Biofunctional Science and Technology, Graduate School of Biosphere Science, Hiroshima Univ, Higashi-Hiroshima, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Murakami
authors.affiliation: Dept. of Biofunctional Science and Technology, Graduate School of Biosphere Science, Hiroshima Univ, Higashi-Hiroshima, Japan.
authors.forename: Soichi
authors.initials: S
authors.lastname: Tanabe
authors.affiliation: Dept. of Biofunctional Science and Technology, Graduate School of Biosphere Science, Hiroshima Univ, Higashi-Hiroshima, Japan.
authors.forename: Takuya
authors.initials: T
authors.lastname: Suzuki
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D001647:Bile Acids and Salts / Q000378:metabolism*; D002432:Cecum / Q000378:metabolism; D002793:Cholic Acids / Q000378:metabolism; D003106:Colon / Q000378:metabolism; D003840:Deoxycholic Acid / Q000378:metabolism; D059305:Diet, High-Fat / Q000009:adverse effects*; D004041:Dietary Fats / Q000008:administration & dosage / Q000009:adverse effects*; D007249:Inflammation / Q000378:metabolism; D007410:Intestinal Diseases / Q000209:etiology* / Q000378:metabolism; D007420:Intestine, Large / Q000378:metabolism / Q000473:pathology*; D062726:Junctional Adhesion Molecules / Q000378:metabolism; D008099:Liver; D008297:Male; D024821:Metabolic Syndrome / Q000473:pathology*; D008810:Mice, Inbred C57BL; D013024:Soybean Oil; D019108:Tight Junctions / Q000378:metabolism*; D015430:Weight Gain; D062827:Zonula Occludens-2 Protein / Q000378:metabolism
chemical_list: D001647:Bile Acids and Salts; D002793:Cholic Acids; D004041:Dietary Fats; D062726:Junctional Adhesion Molecules; D062827:Zonula Occludens-2 Protein; D003840:Deoxycholic Acid; C004821:muricholic acid; D013024:Soybean Oil; C029310:lard
keywords: bile acid; high-fat diet; intestinal permeability; mice; tight junction