title: The Methylated DNA Immunoprecipitation [MeDIP] to Investigate the Epigenetic Remodeling in Cell Fate Determination and Cancer Development.
abstract: Epigenetic mechanisms such as DNA methylation, posttranslational modifications of histone proteins, remodeling of nucleosomes, and the expression of noncoding RNAs contribute to the regulation of gene expression for the cell fate determination and tissue development. The disruption of these epigenetic mechanisms, in conjunction with genetic alterations, is a decisive element for cancer development and progression. The cancer phenotype is characterized by global DNA hypomethylation and gene-specific hypermethylation. The methylated DNA immunoprecipitation [MeDIP] is a useful approach currently used to clarify the functional consequences of DNA methylation on cell fate determination and cancer development.
authors.affiliation: Section of Histology & Medical Embryology, Department of Anatomical, Histological, Forensic & Orthopaedic Sciences, Sapienza University of Rome, Via A. Scarpa, 14-16, 00161, Rome, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Masciarelli
authors.affiliation: Section of Histology & Medical Embryology, Department of Anatomical, Histological, Forensic & Orthopaedic Sciences, Sapienza University of Rome, Via A. Scarpa, 14-16, 00161, Rome, Italy.
authors.forename: Teresa
authors.initials: T
authors.lastname: Bellissimo
authors.affiliation: Section of Histology & Medical Embryology, Department of Anatomical, Histological, Forensic & Orthopaedic Sciences, Sapienza University of Rome, Via A. Scarpa, 14-16, 00161, Rome, Italy.
authors.forename: Ilaria
authors.initials: I
authors.lastname: Iosue
authors.affiliation: Section of Histology & Medical Embryology, Department of Anatomical, Histological, Forensic & Orthopaedic Sciences, Sapienza University of Rome, Via A. Scarpa, 14-16, 00161, Rome, Italy. francesco.fazi@uniroma1.it.
authors.forename: Francesco
authors.initials: F
authors.lastname: Fazi
mesh_terms: D063646:Carcinogenesis / Q000235:genetics*; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D019175:DNA Methylation; D044127:Epigenesis, Genetic; D006801:Humans; D047468:Immunoprecipitation / Q000379:methods*; D009369:Neoplasms / Q000235:genetics* / Q000473:pathology*; D016133:Polymerase Chain Reaction
chemical_list: D004247:DNA
keywords: 5-Methyl-cytosine; Cancer chemoprevention; Chromatin immunoprecipitation; CpG island; DNA methylation; Epigenetic remodeling; MeDIP