title: Characterization of oil-palm trunk residue degradation enzymes derived from the isolated fungus, Penicillium rolfsii c3-2(1) IBRL.
abstract: This study characterizes crude enzymes derived from Penicillium rolfsii c3-2(1) IBRL, a mesophilic fungus isolated from the local soil of Malaysia. Prior to enzyme activity evaluation, P. rolfsii c3-2(1) IBRL was inoculated into a broth medium containing oil-palm trunk residues for the preparation of crude enzymes. Oil-palm trunk residues were optimally hydrolysed at pH5.0 and 50°C. P. rolfsii c3-2(1) IBRL-derived crude enzymes displayed higher thermal stability compared with the commercial enzymes, Celluclast 1.5 L and Acellerase 1500. Moreover, the hydrolysing activities of the P. rolfsii c3-2(1) IBRL-derived crude enzymes (xylan, arabinan, and laminarin) were superior compared to that of Celluclast 1.5 L and Acellerase 1500, and exhibit 2- to 3-fold and 3- to 4-fold higher oil-palm trunk residues-hydrolysing specific activity, respectively. This higher hydrolysis efficiency may be attributed to the weak 'lignin-binding' ability of the P. rolfsii c3-2(1) IBRL-derived enzymes compared to the commercial enzymes.
authors.affiliation: a Department of Biological Science, Faculty of Science , Universiti Tunku Abdul Rahman, Jalan Universiti , Kampar , Malaysia.
authors.forename: Kok Chang
authors.initials: KC
authors.lastname: Lee
authors.affiliation: b Japan International Research Center for Agricultural Sciences (JIRCAS) 1-1 , Tsukuba City , Japan.
authors.forename: Takamitsu
authors.initials: T
authors.lastname: Arai
authors.affiliation: c Industrial Biotechnology Research Laboratory (IBRL), School of Biological Sciences , Universiti Sains Malaysia , Minden , Malaysia.
authors.forename: Darah
authors.initials: D
authors.lastname: Ibrahim
authors.affiliation: b Japan International Research Center for Agricultural Sciences (JIRCAS) 1-1 , Tsukuba City , Japan.
authors.forename: Lan
authors.initials: L
authors.lastname: Deng
authors.affiliation: b Japan International Research Center for Agricultural Sciences (JIRCAS) 1-1 , Tsukuba City , Japan.
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Murata
authors.affiliation: b Japan International Research Center for Agricultural Sciences (JIRCAS) 1-1 , Tsukuba City , Japan.
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Mori
authors.affiliation: b Japan International Research Center for Agricultural Sciences (JIRCAS) 1-1 , Tsukuba City , Japan.
authors.forename: Akihiko
authors.initials: A
authors.lastname: Kosugi
mesh_terms: D028023:Arecaceae / Q000737:chemistry*; D018533:Biomass; D044602:Cellulases / Q000302:isolation & purification / Q000378:metabolism; D005656:Fungal Proteins / Q000302:isolation & purification / Q000378:metabolism; D006868:Hydrolysis; D008031:Lignin / Q000737:chemistry / Q000378:metabolism*; D010407:Penicillium / Q000201:enzymology*; D012037:Refuse Disposal; D013997:Time Factors
chemical_list: D005656:Fungal Proteins; D008031:Lignin; D044602:Cellulases
keywords: Penicillium rolfsii c3-2(1) IBRL; commercial enzymes; lignin; lignocellulosic biomass; oil-palm trunk residues