title: Quantification of Metanephrine and Normetanephrine in Urine Using Liquid Chromatography-Tandem Mass Spectrometry.
abstract: Measuring urinary metanephrines aides in the diagnosis of pheochromocytomas-catecholamine producing tumors. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) allows for greater sensitivity and simpler sample preparation as compared with other techniques. Here we describe a simple LC-MS/MS method for measuring metanephrines in urine. Each urine sample was treated with diphenylboronic acid to create boronate complexes, and then applied to a Bond-Elut Plexa cartridge. After solid phase extraction, samples were concentrated and analyzed on an Atlantis T3 column with chromatographic run time totaling 8.5 min. MS/MS was set in positive electrospray ionization mode with multiple reaction monitoring for data collection. The assay was linear from 0.2 to 27.4 μmol/L and 0.3 to 14.6 μmol/L for metanephrine and normetanephrine, respectively. Intra-assay and total precision at three concentration levels over 10 days were <5 % for metanephrine and <10 % for normetanephrine.
authors.affiliation: ThermoFisher Scientific, West Palm Beach, FL, USA. Jessica.fares@thermofisher.com.
authors.forename: Jessica
authors.initials: J
authors.lastname: Gabler
authors.affiliation: Department of Laboratory Medicine, Cleveland Clinic, Cleveland, OH, USA. wangs2@ccf.org.
authors.forename: Sihe
authors.initials: S
authors.lastname: Wang
mesh_terms: D053000:Analytic Sample Preparation Methods; D001897:Boronic Acids / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005561:Formates / Q000737:chemistry; D006801:Humans; D008676:Metanephrine / Q000737:chemistry / Q000302:isolation & purification / Q000652:urine*; D009647:Normetanephrine / Q000737:chemistry / Q000302:isolation & purification / Q000652:urine*; D052616:Solid Phase Extraction; D053719:Tandem Mass Spectrometry / Q000379:methods*; D016482:Urinalysis / Q000379:methods*
chemical_list: D001897:Boronic Acids; D005561:Formates; D009647:Normetanephrine; C030544:formic acid; D008676:Metanephrine
keywords: Liquid chromatography; Mass spectrometry; Metanephrine; Normetanephrine; Pheochromocytoma; Urine