title: Aberrant sodium influx causes cardiomyopathy and atrial fibrillation in mice.
abstract: Increased sodium influx via incomplete inactivation of the major cardiac sodium channel Na(V)1.5 is correlated with an increased incidence of atrial fibrillation (AF) in humans. Here, we sought to determine whether increased sodium entry is sufficient to cause the structural and electrophysiological perturbations that are required to initiate and sustain AF. We used mice expressing a human Na(V)1.5 variant with a mutation in the anesthetic-binding site (F1759A-Na(V)1.5) and demonstrated that incomplete Na+ channel inactivation is sufficient to drive structural alterations, including atrial and ventricular enlargement, myofibril disarray, fibrosis and mitochondrial injury, and electrophysiological dysfunctions that together lead to spontaneous and prolonged episodes of AF in these mice. Using this model, we determined that the increase in a persistent sodium current causes heterogeneously prolonged action potential duration and rotors, as well as wave and wavelets in the atria, and thereby mimics mechanistic theories that have been proposed for AF in humans. Acute inhibition of the sodium-calcium exchanger, which targets the downstream effects of enhanced sodium entry, markedly reduced the burden of AF and ventricular arrhythmias in this model, suggesting a potential therapeutic approach for AF. Together, our results indicate that these mice will be important for assessing the cellular mechanisms and potential effectiveness of antiarrhythmic therapies.
authors.forename: Elaine
authors.initials: E
authors.lastname: Wan
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Abrams
authors.forename: Richard L
authors.initials: RL
authors.lastname: Weinberg
authors.forename: Alexander N
authors.initials: AN
authors.lastname: Katchman
authors.forename: Joseph
authors.initials: J
authors.lastname: Bayne
authors.forename: Sergey I
authors.initials: SI
authors.lastname: Zakharov
authors.forename: Lin
authors.initials: L
authors.lastname: Yang
authors.forename: John P
authors.initials: JP
authors.lastname: Morrow
authors.forename: Hasan
authors.initials: H
authors.lastname: Garan
authors.forename: Steven O
authors.initials: SO
authors.lastname: Marx
mesh_terms: D000818:Animals; D001281:Atrial Fibrillation / Q000209:etiology*; D002118:Calcium / Q000378:metabolism; D009202:Cardiomyopathies / Q000209:etiology*; D004562:Electrocardiography; D051379:Mice; D062554:NAV1.5 Voltage-Gated Sodium Channel / Q000502:physiology*; D012964:Sodium / Q000378:metabolism*
chemical_list: D062554:NAV1.5 Voltage-Gated Sodium Channel; C568321:Scn5a protein, mouse; D012964:Sodium; D002118:Calcium