title: Development of an HPLC Method for Absolute Quantification and QAMS of Flavonoids Components in Psoralea corylifolia L.
abstract: The seeds of Psoralea corylifolia L. (Fabaceae) are a commonly used medicinal herb in eastern Asia with many beneficial effects in clinical therapies. In this study, a simple, sensitive, precise, and specific reverse phase high-performance liquid chromatography (HPLC) method was established for quantification of 9 flavonoids in P. corylifolia, including isobavachin, neobavaisoflavone, bavachin, corylin, bavachalcone, bavachinin, isobavachalcone, corylifol A, and 4'-O-methylbavachalcone. Based on this method, a quantitative analysis of multicomponents by single marker (QAMS) was carried out, and the relative correction factors (RCFs) were calculated for determining the contents of other flavonoids. The accuracy of QAMS method was verified by comparing with the results of external standard method, as well as the feasibility and adaptability of the method applied on quality control of P. corylifolia. The 9 compounds were baseline separated in 60 min with a good linearity of regression coefficient (R (2)) over 0.9991. The accuracies of QAMS were between 92.89% and 109.5%. The RSD values of f in different injection volume were between 2.3% and 3.6%. The results obtained from QAMS suggested that it was a convenient and accurate method to determine multicomponents especially when some authentic standard substances were unavailable. It can be used to control the quality of P. corylifolia.
authors.affiliation: Jiangsu Key Laboratory of Chinese Medicine Processing, Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing 210023, China ; Pharmacy College of Nanjing University of TCM, Nanjing 210023, China.
authors.forename: Cuiping
authors.initials: C
authors.lastname: Yan
authors.affiliation: Jiangsu Key Laboratory of Chinese Medicine Processing, Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing 210023, China ; Pharmacy College of Nanjing University of TCM, Nanjing 210023, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Jiangsu Key Laboratory of Chinese Medicine Processing, Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing 210023, China ; Pharmacy College of Nanjing University of TCM, Nanjing 210023, China.
authors.forename: Zebin
authors.initials: Z
authors.lastname: Weng
authors.affiliation: Jiangsu Key Laboratory of Chinese Medicine Processing, Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing 210023, China ; Pharmacy College of Nanjing University of TCM, Nanjing 210023, China.
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Gao
authors.affiliation: Jiangsu Key Laboratory of Chinese Medicine Processing, Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing 210023, China ; Pharmacy College of Nanjing University of TCM, Nanjing 210023, China.
authors.forename: Guangming
authors.initials: G
authors.lastname: Yang
authors.affiliation: Jiangsu Key Laboratory of Chinese Medicine Processing, Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing 210023, China ; Pharmacy College of Nanjing University of TCM, Nanjing 210023, China.
authors.forename: Zhipeng
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Jiangsu Key Laboratory of Chinese Medicine Processing, Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing 210023, China ; Pharmacy College of Nanjing University of TCM, Nanjing 210023, China.
authors.forename: Baochang
authors.initials: B
authors.lastname: Cai
authors.affiliation: Jiangsu Key Laboratory of Chinese Medicine Processing, Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing 210023, China ; Pharmacy College of Nanjing University of TCM, Nanjing 210023, China.
authors.forename: Weidong
authors.initials: W
authors.lastname: Li