title: Correlation between endogenous polyamines in human cardiac tissues and clinical parameters in patients with heart failure.
abstract: Polyamines contribute to several physiological and pathological processes, including cardiac hypertrophy in experimental animals. This involves an increase in ornithine decarboxylase (ODC) activity and intracellular polyamines associated with cyclic adenosine monophosphate (cAMP) increases. The aim of the study was to establish the role of these in the human heart in living patients. For this, polyamines (by high performance liquid chromatography) and the activity of ODC and N(1)-acetylpolyamine oxidases (APAO) were determined in the right atrial appendage of 17 patients undergoing extracorporeal circulation to correlate with clinical parameters. There existed enzymatic activity associated with the homeostasis of polyamines. Left atria size was positively associated with ODC (r = 0.661, P = 0.027) and negatively with APAO-N(1) -acetylspermine (r = -0.769, P = 0.026), suggesting that increased levels of polyamines are associated with left atrial hemodynamic overload. Left ventricular ejection fraction (LVEF) and heart rate were positively associated with spermidine (r = 0.690, P = 0.003; r = 0.590, P = 0.021) and negatively with N(1)-acetylspermidine (r = -0.554, P = 0.032; r = -0.644, P = 0.018). LVEF was negatively correlated with cAMP levels (r = -0.835, P = 0.001) and with cAMP/ODC (r = -0.794, P = 0.011), cAMP/spermidine (r = -0.813, P = 0.001) and cAMP/spermine (r = -0.747, P = 0.003) ratios. Abnormal LVEF patients showed decreased ODC activity and spermidine, and increased N(1) -acetylspermidine, and cAMP. Spermine decreased in congestive heart failure patients. The trace amine isoamylamine negatively correlated with septal wall thickness (r = -0.634, P = 0.008) and was increased in cardiac heart failure. The results indicated that modifications in polyamine homeostasis might be associated with cardiac function and remodelling. Increased cAMP might have a deleterious effect on function. Further studies should confirm these findings and the involvement of polyamines in different stages of heart failure.
authors.affiliation: Farmacología, Departamento de Medicina, Universidad de Oviedo, Oviedo, Spain.
authors.forename: Clara
authors.initials: C
authors.lastname: Meana
authors.affiliation: Servicio de Cardiología, Hospital Universitario Central de Asturias, Asturias, Spain.
authors.forename: José Manuel
authors.initials: JM
authors.lastname: Rubín
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Universidad de Oviedo, Oviedo, Spain.|Instituto Universitario de Oncología del Principado de Asturias, Asturias, Spain.
authors.forename: Carmen
authors.initials: C
authors.lastname: Bordallo
authors.affiliation: Instituto Universitario de Oncología del Principado de Asturias, Asturias, Spain.
authors.forename: Lorena
authors.initials: L
authors.lastname: Suárez
authors.affiliation: Farmacología, Departamento de Medicina, Universidad de Oviedo, Oviedo, Spain.|Instituto Universitario de Oncología del Principado de Asturias, Asturias, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: Bordallo
authors.affiliation: Farmacología, Departamento de Medicina, Universidad de Oviedo, Oviedo, Spain.|Instituto Universitario de Oncología del Principado de Asturias, Asturias, Spain.
authors.forename: Manuel
authors.initials: M
authors.lastname: Sánchez
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D006321:Heart / Q000503:physiopathology*; D006333:Heart Failure / Q000378:metabolism* / Q000473:pathology; D006339:Heart Rate / Q000502:physiology; D006801:Humans; D008297:Male; D008875:Middle Aged; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D009955:Ornithine Decarboxylase / Q000378:metabolism; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000378:metabolism; D011073:Polyamines / Q000378:metabolism*
chemical_list: D011073:Polyamines; D000587:Oxidoreductases Acting on CH-NH Group Donors; C501650:N(1)-acetylpolyamine oxidase, human; D009955:Ornithine Decarboxylase
keywords: N1-acetylpolyamine oxidase; atria; cyclic AMP; heart; heart failure; ornithine decarboxylase; polyamines; putrescine; spermidine; spermine