title: Molecular cloning and functional characterization of duck mitochondrial antiviral-signaling protein (MAVS).
abstract: Mitochondrial antiviral-signaling protein (MAVS), also called IPS-1/VISA/Cardif, is an important molecule involved in host defense and triggers a signal for producing type I IFN. Currently the function of MAVS in ducks (duMAVS) remains largely unclear while significant progress has been made in mammals. In this study, the full-length duMAVS cDNA was cloned from duck embryo fibroblasts (DEFs) for the first time. Tissue specificity analysis showed duMAVS was universally expressed in all detected tissues. DEFs transfected with duMAVS were able to induce interferon-β (IFN-β) expression through activating interferon regulatory factor 1 (IRF1) and nuclear factor kappa B (NF-κB). Both the CARD-like domain and transmembrane domain were required for duMAVS signaling via deletion mutant analysis. In addition, poly(I:C)- or Sendai virus (SeV)-induced IFN-β expression in DEFs were significantly decreased by knock-down of duMAVS with siRNA. Altogether, these results indicate that MAVS is a critical immunoregulator in duck innate immune system.
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Huilin
authors.initials: H
authors.lastname: Li
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Yajun
authors.initials: Y
authors.lastname: Zhai
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Yufang
authors.initials: Y
authors.lastname: Fan
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Huanchun
authors.initials: H
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Anding
authors.initials: A
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Jin
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. Electronic address: luorui@mail.hzau.edu.cn.
authors.forename: Rui
authors.initials: R
authors.lastname: Luo
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000737:chemistry / Q000276:immunology*; D000595:Amino Acid Sequence; D000818:Animals; D030161:Avian Proteins / Q000737:chemistry / Q000276:immunology*; D001715:Bird Diseases / Q000276:immunology* / Q000821:virology; D004372:Ducks; D004625:Embryo, Nonmammalian / Q000166:cytology; D005347:Fibroblasts / Q000276:immunology; D050856:Interferon Regulatory Factor-1 / Q000378:metabolism; D016899:Interferon-beta / Q000276:immunology; D008969:Molecular Sequence Data; D016328:NF-kappa B / Q000378:metabolism; D009928:Organ Specificity; D010802:Phylogeny; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment; D014158:Transcription, Genetic; D014777:Virus Diseases / Q000276:immunology / Q000662:veterinary* / Q000821:virology
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D030161:Avian Proteins; D050856:Interferon Regulatory Factor-1; D016328:NF-kappa B; D016899:Interferon-beta
keywords: Duck; Interferon-β; MAVS