title: Creating Ribo-T: (Design, Build, Test)n.
abstract: Engineering biology is especially challenging given our relatively poor ability to rationally design within life's complex design landscape. Thus, moving through the engineering "design, build, test" cycle multiple times accumulates system knowledge and hopefully yields a successful design. Here I discuss the engineering process behind our recently published work creating a ribosome with tethered subunits, Ribo-T.
authors.affiliation: Department of Chemical and Biological Engineering, Northwestern University , 2145 Sheridan Road, Tech E-136, Evanston, Illinois 60208, United States.|Chemistry of Life Processes Institute, Northwestern University , 2170 Campus Drive, Evanston, Illinois 60208, United States.
authors.forename: Erik D
authors.initials: ED
authors.lastname: Carlson
mesh_terms: D012270:Ribosomes / Q000737:chemistry / Q000378:metabolism*; D058615:Synthetic Biology