title: IgA and IgG1 reactivities assessed by flow cytometry mirror clinical aspects of infants with ocular congenital toxoplasmosis.
abstract: This study intended to apply the flow cytometric analysis of IgA and IgG reactivity and intracytoplasmic cytokine analysis to understand and decode the clinical aspects of infants with ocular congenital toxoplasmosis. The Toxoplasma gondii-infected infants (TOXO) were subdivided according to their clinical aspects based on the absence (NRL), presence of active (ARL), active/cicatricial (ACRL) or cicatricial retinochoroidal lesions (CRL) and compared to non-infected controls (NI). The reactivity of anti-T. gondii IgG subclasses resembles the clinical aspects of ocular lesions. IgG and IgG1 discriminate infants with cicatricial lesions (ACRL and CRL) from both ARL and NLR. IgG2 and IgG3 are particularly higher in ACRL and CRL as compared to NLR. No differences were observed when IgG4 reactivity was evaluated. Thus, the results indicated that the reactivity patterns of IgA, IgG and IgG subclasses are able to discriminate ARL, ACRL and CRL from NLR or NI. IgA and IgG subclasses are relevant serological biomarkers with diagnostic and prognostic applicability, respectively. Moreover, IgA and IgG1 were closely related to cytokine production by innate/adaptive immunity cells. IgA reactivity was directly associated to TNF-α-derived from neutrophils, monocytes and CD8(+) T-cells, while IgG1 was inversely correlated with IFN-γ-producing CD4(+) and CD8(+) T-cells but positively correlated with IL-10(+) B-cells. These findings provide insights on the relationship between the cytokine production by innate/adaptive immunity and the antibody pattern of infants with ocular congenital toxoplasmosis. In addition, the present study supports the use of flow cytometric serology as a potential tool for the diagnosis and monitoring of ocular lesions in T. gondii-infected infants in the clinical setting.
authors.affiliation: Universidade Federal do Espírito Santo, Vitória, ES, Brazil.
authors.forename: Laura Néspoli Nassar Pansini
authors.initials: LN
authors.lastname: de Jesus
authors.affiliation: Universidade Federal do Espírito Santo, Vitória, ES, Brazil.
authors.forename: Aline de Castro Zacche
authors.initials: Ade C
authors.lastname: Tonini
authors.affiliation: Universidade Federal do Espírito Santo, Vitória, ES, Brazil.
authors.forename: Geisa Baptista
authors.initials: GB
authors.lastname: Barros
authors.affiliation: Centro de Pesquisas René Rachou, FIOCRUZ, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Jordana Grazziela A
authors.initials: JG
authors.lastname: Coelho-dos-Reis
authors.affiliation: Centro de Pesquisas René Rachou, FIOCRUZ, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Samantha Ribeiro
authors.initials: SR
authors.lastname: Béla
authors.affiliation: Centro de Pesquisas René Rachou, FIOCRUZ, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Lis Ribeiro do Valle
authors.initials: LR
authors.lastname: Antonelli
authors.affiliation: Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Anderson Silva
authors.initials: AS
authors.lastname: Machado
authors.affiliation: Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Ana Carolina Aguiar Vasconcelos
authors.initials: AC
authors.lastname: Carneiro
authors.affiliation: Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Gláucia Manzan Queiroz
authors.initials: GM
authors.lastname: Andrade
authors.affiliation: Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Daniel Vitor
authors.initials: DV
authors.lastname: Vasconcelos-Santos
authors.affiliation: Núcleo de Ações e pesquisa em Apoio Diagnóstico (NUPAD), Belo Horizonte, Minas Gerais, Brazil.
authors.forename: José Nélio
authors.initials: JN
authors.lastname: Januário
authors.affiliation: Centro de Pesquisas René Rachou, FIOCRUZ, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Andréa
authors.initials: A
authors.lastname: Teixeira-Carvalho
authors.affiliation: Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Ricardo Wagner Almeida
authors.initials: RW
authors.lastname: Vitor
authors.affiliation: Universidade Federal de Uberlândia, Minas Gerais, Brazil.
authors.forename: Eloísa A V
authors.initials: EA
authors.lastname: Ferro
authors.affiliation: Universidade Federal de Uberlândia, Minas Gerais, Brazil.
authors.forename: José Roberto
authors.initials: JR
authors.lastname: Mineo
authors.affiliation: Universidade Federal do Rio de Janeiro, Macaé, Minas Gerais, Brazil.
authors.forename: Lilian Maria Garcia
authors.initials: LM
authors.lastname: Bahia-Oliveira
authors.affiliation: Centro de Pesquisas René Rachou, FIOCRUZ, Belo Horizonte, Minas Gerais, Brazil. Electronic address: oassismartins@gmail.com.
authors.forename: Olindo Assis
authors.initials: OA
authors.lastname: Martins-Filho
authors.affiliation: Universidade Federal do Espírito Santo, Vitória, ES, Brazil.
authors.forename: Elenice Moreira
authors.initials: EM
authors.lastname: Lemos
mesh_terms: D003430:Cross-Sectional Studies; D016207:Cytokines / Q000276:immunology; D005434:Flow Cytometry; D006801:Humans; D007070:Immunoglobulin A / Q000276:immunology*; D007074:Immunoglobulin G / Q000276:immunology*; D007223:Infant; D011446:Prospective Studies; D014125:Toxoplasmosis, Congenital / Q000175:diagnosis / Q000276:immunology*
chemical_list: D016207:Cytokines; D007070:Immunoglobulin A; D007074:Immunoglobulin G
keywords: Cytokines; IgA; IgG1; Ocular congenital toxoplasmosis; T. gondii