title: Bufavirus Protoparvovirus in feces of wild rats in China.
abstract: Bufavirus (BuV) was first discovered from feces of children with acute diarrhea. It was subsequently detected from several animal species including shrews, bats, and nonhuman primates. In this study, we identified a novel Protoparvovirus, designated RatBuV, from the intestinal contents of wild rats using viral metagenomics. The near complete genome was 4643 nt encoding NS1, VP1, and VP2 proteins. Phylogenetic analysis over the complete genome showed that RatBuV clustered with Mpulungu BuV from shrews. Sequence analysis indicated that the putative protein sequences of NS1, VP1, and VP2 of RatBuV shared identities of 50.6-77.2, 48.3-77.3, and 47.1-78.3 %, respectively, with those of human BuVs, MpBuV, and WUHARV parvovirus, suggesting RatBuV belongs to a new species of Protoparvovirus. Our epidemiologic study indicated that the prevalence rate of RatBuV in the cohort of 40 wild rats is 12.5 % (5/40), which is higher than that of BuV in humans in a previous study.
authors.affiliation: School of Medicine, Jiangsu University, Zhenjiang, People's Republic of China.
authors.forename: Shixing
authors.initials: S
authors.lastname: Yang
authors.affiliation: College of Biology and the Environment, Nanjing Forestry University, Nanjing, People's Republic of China.
authors.forename: Dawei
authors.initials: D
authors.lastname: Liu
authors.affiliation: School of Medicine, Jiangsu University, Zhenjiang, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Interventional Medicine, Yantai Affiliated Hospital of Binzhou Medical University, Yantai, People's Republic of China.
authors.forename: Fanyong
authors.initials: F
authors.lastname: Qu
authors.affiliation: Division of Acute Infectious Disease Prevention and Control, Taizhou Center for Disease Control and Prevention, Taizhou, People's Republic of China.
authors.forename: Yilin
authors.initials: Y
authors.lastname: He
authors.affiliation: School of Medicine, Jiangsu University, Zhenjiang, People's Republic of China.
authors.forename: Zixuan
authors.initials: Z
authors.lastname: Sun
authors.affiliation: School of Medicine, Jiangsu University, Zhenjiang, People's Republic of China.
authors.forename: Quan
authors.initials: Q
authors.lastname: Shen
authors.affiliation: School of Medicine, Jiangsu University, Zhenjiang, People's Republic of China.
authors.forename: Wang
authors.initials: W
authors.lastname: Li
authors.affiliation: School of Medicine, Jiangsu University, Zhenjiang, People's Republic of China.
authors.forename: Xingli
authors.initials: X
authors.lastname: Fu
authors.affiliation: Department of Laboratory Medicine, Blood Systems Research Institute, University of California San Francisco, San Francisco, CA, USA.
authors.forename: Xutao
authors.initials: X
authors.lastname: Deng
authors.affiliation: School of Medicine, Jiangsu University, Zhenjiang, People's Republic of China. z0216wen@yahoo.com.
authors.forename: Wen
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Laboratory Medicine, Blood Systems Research Institute, University of California San Francisco, San Francisco, CA, USA.
authors.forename: Eric
authors.initials: E
authors.lastname: Delwart
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000835:Animals, Wild / Q000821:virology; D002681:China; D005243:Feces / Q000821:virology; D016679:Genome, Viral; D007422:Intestines / Q000821:virology; D008969:Molecular Sequence Data; D010322:Parvoviridae Infections / Q000821:virology; D019219:Parvovirinae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D010802:Phylogeny; D051381:Rats / Q000821:virology*; D016415:Sequence Alignment; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D014764:Viral Proteins
keywords: Bufavirus; Genome structure; Phylogentic analysis; Wild rats