title: Start2Fold: a database of hydrogen/deuterium exchange data on protein folding and stability.
abstract: Proteins fulfil a wide range of tasks in cells; understanding how they fold into complex three-dimensional (3D) structures and how these structures remain stable while retaining sufficient dynamics for functionality is essential for the interpretation of overall protein behaviour. Since the 1950's, solvent exchange-based methods have been the most powerful experimental means to obtain information on the folding and stability of proteins. Considerable expertise and care were required to obtain the resulting datasets, which, despite their importance and intrinsic value, have never been collected, curated and classified. Start2Fold is an openly accessible database (http://start2fold.eu) of carefully curated hydrogen/deuterium exchange (HDX) data extracted from the literature that is open for new submissions from the community. The database entries contain (i) information on the proteins investigated and the underlying experimental procedures and (ii) the classification of the residues based on their exchange protection levels, also allowing for the instant visualization of the relevant residue groups on the 3D structures of the corresponding proteins. By providing a clear hierarchical framework for the easy sharing, comparison and (re-)interpretation of HDX data, Start2Fold intends to promote a better understanding of how the protein sequence encodes folding and structure as well as the development of new computational methods predicting protein folding and stability.
authors.affiliation: Structural Biology Brussels, Vrije Universiteit Brussel (VUB), Brussels 1050, Belgium Structural Biology Research Center (IB), VIB, Brussels 1050, Belgium rpancsa@mrc-lmb.cam.ac.uk.
authors.forename: Rita
authors.initials: R
authors.lastname: Pancsa
authors.affiliation: Structural Biology Brussels, Vrije Universiteit Brussel (VUB), Brussels 1050, Belgium Structural Biology Research Center (IB), VIB, Brussels 1050, Belgium mvaradi@ebi.ac.uk.
authors.forename: Mihaly
authors.initials: M
authors.lastname: Varadi
authors.affiliation: Structural Biology Brussels, Vrije Universiteit Brussel (VUB), Brussels 1050, Belgium Structural Biology Research Center (IB), VIB, Brussels 1050, Belgium Interuniversity Institute of Bioinformatics in Brussels (IB), ULB-VUB, Brussels 1050, Belgium Institute of Enzymology, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Budapest 1113, Hungary.
authors.forename: Peter
authors.initials: P
authors.lastname: Tompa
authors.affiliation: Structural Biology Brussels, Vrije Universiteit Brussel (VUB), Brussels 1050, Belgium Structural Biology Research Center (IB), VIB, Brussels 1050, Belgium Interuniversity Institute of Bioinformatics in Brussels (IB), ULB-VUB, Brussels 1050, Belgium wvranken@vub.ac.be.
authors.forename: Wim F
authors.identifier: http://orcid.org/0000-0001-7470-4324
authors.initials: WF
authors.lastname: Vranken
mesh_terms: D030562:Databases, Protein; D041961:Deuterium Exchange Measurement; D011487:Protein Conformation; D017510:Protein Folding; D055550:Protein Stability; D020539:Sequence Analysis, Protein