title: Photodynamic therapy via FRET following bioorthogonal click reaction in cancer cells.
abstract: Longer wavelength light (650-800nm) is desired to treat large tumors in photodynamic therapy (PDT). However, shorter wavelength light is needed in PDT for thin tumors, not to cause undesirable local side effects. We proposed a strategy for stepwise optical imaging and PDT using a bioorthogonal click chemistry and fluorescence resonance energy transfer (FRET). We prepared azidyl rhodamine (Rh-N3, clickable FD) and cyclooctynyl phthalocyanine [Pc-(DIBAC), clickable PS], with which, here, we demonstrate that the non-catalytic click chemistry is rapid and efficient in cancer cells and FRET from a fluorescence dye (FD) to a photosensitizer (PS) is sufficient to generate enough singlet oxygen killing cancer cells by using shorter wavelength light.
authors.affiliation: Department of Pharmaceutical Sciences, College of Pharmacy, University of Oklahoma, Oklahoma City, OK 73117, United States.
authors.forename: Moses
authors.initials: M
authors.lastname: Bio
authors.affiliation: Department of Pharmaceutical Sciences, College of Pharmacy, University of Oklahoma, Oklahoma City, OK 73117, United States.
authors.forename: Pallavi
authors.initials: P
authors.lastname: Rajaputra
authors.affiliation: Department of Pharmaceutical Sciences, College of Pharmacy, University of Oklahoma, Oklahoma City, OK 73117, United States; Department of Chemistry and Biochemistry, University of Oklahoma, Norman, OK 73019, United States.
authors.forename: Youngjae
authors.initials: Y
authors.lastname: You
mesh_terms: D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D057930:Click Chemistry; D004305:Dose-Response Relationship, Drug; D031541:Fluorescence Resonance Energy Transfer; D006801:Humans; D015394:Molecular Structure; D009369:Neoplasms / Q000473:pathology*; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D013329:Structure-Activity Relationship
chemical_list: D017319:Photosensitizing Agents
keywords: Bioorthogonal click reaction; Fluorescence resonance energy transfer; Non-muscle invasive bladder cancer; Photodynamic therapy