title: Chemical interactions and gel properties of black carp actomyosin affected by MTGase and their relationships.
abstract: Partial least squares regression (PLSR) was applied to evaluate and correlate chemical interactions (-NH2 content, S-S bonds, four non-covalent interactions) with gel properties (dynamic rheological properties and cooking loss (CL)) of black carp actomyosin affected by microbial transglutaminase (MTGase) at suwari and kamaboko stages. The G' and CL were significantly enhanced by MTGase and their values in kamaboko gels were higher than those in suwari gels at the same MTGase concentration. The γ-carboxyamide and amino cross-links, catalyzed by MTGase, were constructed at suwari stage and contributed to the network formation, while disulfide bonds were formed not only in suwari gels but also in kamaboko gels, further enhancing the gel network. PLSR analysis revealed that 86.6-90.3% of the variation of G' and 91.8-94.4% of the variation of CL were best explained by chemical interactions. G' mainly depended on covalent cross-links and gave positive correlation. CL was positively correlated with covalent cross-links, but negatively related to non-covalent bonds, indicating that covalent bonds promoted water extrusion, whereas non-covalent bonds were beneficial for water-holding.
authors.affiliation: College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; The Sub Center (Wuhan) of National Technology and R&D of Staple Freshwater Fish Processing, Wuhan 430070, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Jia
authors.affiliation: College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; The Sub Center (Wuhan) of National Technology and R&D of Staple Freshwater Fish Processing, Wuhan 430070, China. Electronic address: hql@mail.hzau.edu.cn.
authors.forename: Qilin
authors.initials: Q
authors.lastname: Huang
authors.affiliation: College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; The Sub Center (Wuhan) of National Technology and R&D of Staple Freshwater Fish Processing, Wuhan 430070, China. Electronic address: xiongsb@mail.hzau.edu.cn.
authors.forename: Shanbai
authors.initials: S
authors.lastname: Xiong
mesh_terms: D000205:Actomyosin / Q000737:chemistry / Q000378:metabolism*; D000818:Animals; D002347:Carps / Q000378:metabolism*; D029941:Fish Proteins / Q000378:metabolism; D005782:Gels / Q000737:chemistry*; D012212:Rheology; D011503:Transglutaminases / Q000378:metabolism*
chemical_list: D029941:Fish Proteins; D005782:Gels; D000205:Actomyosin; D011503:Transglutaminases
keywords: Black carp actomyosin; Chemical interactions; Gel properties; MTGase; PLSR