title: Direct Analysis of Gene Synthesis Reactions Using Solid-State Nanopores.
abstract: Synthetic nucleic acids offer rich potential to understand and engineer new cellular functions, yet an unresolved limitation in their production and usage is deleterious products, which restrict design complexity and add cost. Herein, we employ a solid-state nanopore to differentiate molecules of a gene synthesis reaction into categories of correct and incorrect assemblies. This new method offers a solution that provides information on gene synthesis reactions in near-real time with higher complexity and lower costs. This advance can permit insights into gene synthesis reactions such as kinetics monitoring, real-time tuning, and optimization of factors that drive reaction-to-reaction variations as well as open venues between nanopore-sensing, synthetic biology, and DNA nanotechnology.
authors.affiliation: Department of Physics, Northeastern University , Boston, Massachusetts 02115, United States.
authors.forename: Spencer
authors.initials: S
authors.lastname: Carson
authors.affiliation: Massachusetts Institute of Technology Lincoln Laboratory, Massachusetts Institute of Technology , 244 Wood Street, Lexington, Massachusetts 02420, United States.
authors.forename: Scott T
authors.initials: ST
authors.lastname: Wick
authors.affiliation: Massachusetts Institute of Technology Lincoln Laboratory, Massachusetts Institute of Technology , 244 Wood Street, Lexington, Massachusetts 02420, United States.
authors.forename: Peter A
authors.initials: PA
authors.lastname: Carr
authors.affiliation: Department of Physics, Northeastern University , Boston, Massachusetts 02115, United States.|Department of Chemistry and Chemical Biology, Northeastern University , Boston, Massachusetts 02115, United States.
authors.forename: Meni
authors.initials: M
authors.lastname: Wanunu
authors.affiliation: Massachusetts Institute of Technology Lincoln Laboratory, Massachusetts Institute of Technology , 244 Wood Street, Lexington, Massachusetts 02420, United States.
authors.forename: Carlos A
authors.initials: CA
authors.lastname: Aguilar
mesh_terms: D004247:DNA / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D005796:Genes / Q000235:genetics*; D016333:HIV Protease / Q000235:genetics / Q000378:metabolism; D007700:Kinetics; D058608:Nanopores; D036103:Nanotechnology / Q000379:methods*; D017422:Sequence Analysis, DNA / Q000379:methods*; D058615:Synthetic Biology / Q000379:methods*
chemical_list: D004247:DNA; D016333:HIV Protease
keywords: DNA; HIV protease; single molecule; synthetic biology; translocation