title: Relationship between the blood perfusion values determined by laser speckle imaging and laser Doppler imaging in normal skin and port wine stains.
abstract: OBJECTIVE
  Laser Doppler imaging (LDI) and laser speckle imaging (LSI) are two major optical techniques aiming at non-invasively imaging the skin blood perfusion. However, the relationship between perfusion values determined by LDI and LSI has not been fully explored.
  
  
  METHODS
  8 healthy volunteers and 13 PWS patients were recruited. The perfusions in normal skin on the forearm of 8 healthy volunteers were simultaneously measured by both LDI and LSI during post-occlusive reactive hyperemia (PORH). Furthermore, the perfusions of port wine stains (PWS) lesions and contralateral normal skin of 10 PWS patients were also determined. In addition, the perfusions for PWS lesions from 3 PWS patients were successively monitored at 0, 10 and 20min during vascular-targeted photodynamic therapy (V-PDT). The average perfusion values determined by LSI were compared with those of LDI for each subject.
  
  
  RESULTS
  In the normal skin during PORH, power function provided better fits of perfusion values than linear function: powers for individual subjects go from 1.312 to 1.942 (R(2)=0.8967-0.9951). There was a linear relationship between perfusion values determined by LDI and LSI in PWS and contralateral normal skin (R(2)=0.7308-0.9623), and in PWS during V-PDT (R(2)=0.8037-0.9968).
  
  
  CONCLUSIONS
  The perfusion values determined by LDI and LSI correlate closely in normal skin and PWS over a broad range of skin perfusion. However, it still suggests that perfusion range and characteristics of the measured skin should be carefully considered if LDI and LSI measures are compared.
authors.affiliation: School of Information and Electronics, Beijing Institute of Technology, Beijing 100081, China.
authors.forename: Defu
authors.initials: D
authors.lastname: Chen
authors.affiliation: Department of Laser Medicine, Chinese People's Liberation Army General Hospital, Beijing 100853, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Ren
authors.affiliation: Department of Laser Medicine, Chinese People's Liberation Army General Hospital, Beijing 100853, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Laser Medicine, Chinese People's Liberation Army General Hospital, Beijing 100853, China.
authors.forename: Hongyou
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Optoelectronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory for Photonics Technology, Fujian Normal University, Fujian 350007, China.
authors.forename: Buhong
authors.initials: B
authors.lastname: Li
authors.affiliation: Department of Laser Medicine, Chinese People's Liberation Army General Hospital, Beijing 100853, China. Electronic address: guyinglaser301@163.com.
authors.forename: Ying
authors.initials: Y
authors.lastname: Gu
mesh_terms: D000293:Adolescent; D000328:Adult; D001783:Blood Flow Velocity; D002648:Child; D005260:Female; D006801:Humans; D017078:Laser-Doppler Flowmetry / Q000379:methods*; D008297:Male; D055420:Perfusion Imaging / Q000379:methods*; D019339:Port-Wine Stain / Q000000981:diagnostic imaging / Q000503:physiopathology*; D012016:Reference Values; D012031:Refractometry / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012867:Skin / Q000098:blood supply / Q000000981:diagnostic imaging / Q000503:physiopathology*; D055815:Young Adult
keywords: Laser Doppler imaging; Laser speckle imaging; Port wine stains; Post-occlusive reactive hyperemia; Skin blood perfusion