title: Serum from patients with ankylosing spondylitis can increase PPARD, fra-1, MMP7, OPG and RANKL expression in MG63 cells.
abstract: OBJECTIVE
  To explore the effects of serum from patients with ankylosing spondylitis on the canonical Wnt/β-catenin pathway and to assess whether the serum has an osteogenic effect in MG63 cells.
  
  
  METHODS
  MG63 cells were cultured with serum from 45 ankylosing spondylitis patients, 30 healthy controls, or 45 rheumatoid arthritis patients. The relative PPARD, fra-1, MMP7, OPG and RANKL mRNA levels were measured using quantitative real-time polymerase chain reaction. Associations between gene expression and patient demographics and clinical assessments were then analyzed.
  
  
  RESULTS
  MG63 cells treated with serum from ankylosing spondylitis patients had higher PPARD, fra-1, MMP7 and OPG gene expression than did cells treated with serum from controls or rheumatoid arthritis patients (all p<0.05). RANKL expression was higher in MG63 cells treated with serum from patients with ankylosing spondylitis or rheumatoid arthritis than in those treated with serum from controls (both p<0.05). The OPG/RANKL ratio was also higher in MG63 cells treated with serum from ankylosing spondylitis patients than in those treated with serum from controls (p<0.05). No associations were found between the expression of the five genes and the patient demographics and clinical assessments (all p>0.05).
  
  
  CONCLUSIONS
  Serum from ankylosing spondylitis patients increases PPARD, fra-1, MMP7, OPG and RANKL expression and the OPG/RANKL ratio in MG63 cells; these effects may be due to the stimulatory effect of the serum on the Wnt pathway.
authors.affiliation: Department of Rheumatology, Sun Yat-Sen University, Guangzhou, China.
authors.forename: Zaiying
authors.initials: Z
authors.lastname: Hu
authors.affiliation: Department of Rheumatology, Sun Yat-Sen University, Guangzhou, China.
authors.forename: Dongfang
authors.initials: D
authors.lastname: Lin
authors.affiliation: Department of Rheumatology, Sun Yat-Sen University, Guangzhou, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Qi
authors.affiliation: Department of Rheumatology, Sun Yat-Sen University, Guangzhou, China.
authors.forename: Minli
authors.initials: M
authors.lastname: Qiu
authors.affiliation: Department of Rheumatology, Sun Yat-Sen University, Guangzhou, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Lv
authors.affiliation: Department of Rheumatology, Sun Yat-Sen University, Guangzhou, China.
authors.forename: Qiuxia
authors.initials: Q
authors.lastname: Li
authors.affiliation: Department of Rheumatology, Sun Yat-Sen University, Guangzhou, China.
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Lin
authors.affiliation: Department of Rheumatology, Sun Yat-Sen University, Guangzhou, China.
authors.forename: Zetao
authors.initials: Z
authors.lastname: Liao
authors.affiliation: Department of Rheumatology, Sun Yat-Sen University, Guangzhou, China.
authors.forename: Yunfeng
authors.initials: Y
authors.lastname: Pan
authors.affiliation: Department of Rheumatology, Sun Yat-Sen University, Guangzhou, China.
authors.forename: Ou
authors.initials: O
authors.lastname: Jin
authors.affiliation: Department of Rheumatology, Sun Yat-Sen University, Guangzhou, China.
authors.forename: Yuqiong
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Rheumatology, Sun Yat-Sen University, Guangzhou, China.
authors.forename: Jieruo
authors.initials: J
authors.lastname: Gu
mesh_terms: D000328:Adult; D001172:Arthritis, Rheumatoid / Q000097:blood*; D002478:Cells, Cultured; D003470:Culture Media; D016207:Cytokines / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D006801:Humans; D008297:Male; D020783:Matrix Metalloproteinase 7 / Q000378:metabolism; D008875:Middle Aged; D010006:Osteoblasts / Q000378:metabolism*; D047494:PPAR delta / Q000378:metabolism; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism; D053245:RANK Ligand / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D044967:Serum; D013167:Spondylitis, Ankylosing / Q000097:blood*; D060449:Wnt Signaling Pathway / Q000235:genetics; D055815:Young Adult; D051176:beta Catenin / Q000235:genetics; D000099169:Fos-Related Antigen 1
chemical_list: D003470:Culture Media; D016207:Cytokines; D020783:Matrix Metalloproteinase 7; D047494:PPAR delta; D016760:Proto-Oncogene Proteins c-fos; D053245:RANK Ligand; D051176:beta Catenin; D000099169:Fos-Related Antigen 1; C080494:osteoclastpoietic factor; C060932:MMP7 protein, human