title: Direct Measurement of Free Estradiol in Human Serum and Plasma by Equilibrium Dialysis-Liquid Chromatography-Tandem Mass Spectrometry.
abstract: We describe a direct method of measurement of free estradiol using equilibrium dialysis followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Serum aliquots and internal standards are extracted by liquid-liquid extraction using methyl-tert-butyl ether (MTBE) followed by derivatization with dansyl chloride. An API 5500 mass spectrometer operated in positive electrospray mode is used for detection.
authors.affiliation: ARUP Institute for Clinical and Experimental Pathology, 500 Chipeta Way, Salt Lake City, UT, 84108, USA. julie.ray@aruplab.com.
authors.forename: Julie A
authors.initials: JA
authors.lastname: Ray
authors.affiliation: ARUP Institute for Clinical and Experimental Pathology, 500 Chipeta Way, Salt Lake City, UT, 84108, USA. kushnmm@aruplab.com.
authors.forename: Mark M
authors.initials: MM
authors.lastname: Kushnir
authors.affiliation: ARUP Institute for Clinical and Experimental Pathology, 500 Chipeta Way, Salt Lake City, UT, 84108, USA. alan.rockwood@aruplab.com.|Department of Pathology, University of Utah School of Medicine, Salt Lake City, UT, USA. alan.rockwood@aruplab.com.
authors.forename: Alan L
authors.initials: AL
authors.lastname: Rockwood
authors.affiliation: ARUP Institute for Clinical and Experimental Pathology, 500 Chipeta Way, Salt Lake City, UT, 84108, USA.|Department of Pathology, University of Utah, Salt Lake City, UT, USA.|Department of Medicine, University of Utah, Salt Lake City, UT, USA.
authors.forename: A Wayne
authors.initials: AW
authors.lastname: Meikle
mesh_terms: D001774:Blood Chemical Analysis / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods*; D004958:Estradiol / Q000097:blood* / Q000737:chemistry / Q000302:isolation & purification; D006801:Humans; D059625:Liquid-Liquid Extraction; D008738:Methyl Ethers / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D008738:Methyl Ethers; C043243:methyl tert-butyl ether; D004958:Estradiol
keywords: Derivatization; Equilibrium dialysis; Free estradiol; LC-MS/MS; SHBG