title: Paecilomyces niveus Stolk & Samson, 1971 (Ascomycota: Thermoascaceae) as a pathogen of Nasonovia ribisnigri (Mosley, 1841) (Hemiptera, Aphididae) in Brazil.
abstract: Nasonovia ribisnigri is a key pest of lettuce (Lactuca sativa L.) in Brazil that requires alternative control methods to synthetic pesticides. We report, for the first time, the occurrence of Paecilomyces niveus as an entomopathogen of the aphid Nasonovia ribisnigri in Pinhais, Paraná, Brazil. Samples of mummified aphids were collected from lettuce crops. The fungus P. niveus (PaePR) was isolated from the insect bodies and identified by macro and micromorphology. The species was confirmed by sequencing Internal Transcribed Spacer (ITS) rDNA. We obtained a sequence of 528 bp (accession number HQ441751), which aligned with Byssochlamys nivea strains (100% identities). In a bioassay, 120 h after inoculation of N. ribisnigri with pathogenic P. niveus had an average mortality of 74%. The presence of P. niveus as a natural pathogen of N. ribisnigri in Brazil suggests that it may be possible to employ P. niveus to minimize the use of chemical insecticides.
authors.affiliation: Departamento de Patologia Básica, Universidade Federal do Paraná, Curitiba, PR, Brazil.
authors.forename: M A C
authors.initials: MA
authors.lastname: Zawadneak
authors.affiliation: Departamento de Patologia Básica, Universidade Federal do Paraná, Curitiba, PR, Brazil.
authors.forename: I C
authors.initials: IC
authors.lastname: Pimentel
authors.affiliation: Departamento de Patologia Básica, Universidade Federal do Paraná, Curitiba, PR, Brazil.
authors.forename: D
authors.initials: D
authors.lastname: Robl
authors.affiliation: Departamento de Patologia Básica, Universidade Federal do Paraná, Curitiba, PR, Brazil.
authors.forename: P
authors.initials: P
authors.lastname: Dalzoto
authors.affiliation: Departamento de Patologia Básica, Universidade Federal do Paraná, Curitiba, PR, Brazil.
authors.forename: V
authors.initials: V
authors.lastname: Vicente
authors.affiliation: Laboratório de Entomologia, Embrapa Soja, Londrina, PR, Brazil.
authors.forename: D R
authors.initials: DR
authors.lastname: Sosa-Gómez
authors.affiliation: Departamento de Patologia Básica, Universidade Federal do Paraná, Curitiba, PR, Brazil.
authors.forename: M
authors.initials: M
authors.lastname: Porsani
authors.affiliation: Departamento de Fitotecnia e Fitossanitarismo, Universidade Federal do Paraná, Curitiba, PR, Brazil.
authors.forename: F L
authors.initials: FL
authors.lastname: Cuquel
mesh_terms: D000818:Animals; D001042:Aphids / Q000254:growth & development / Q000382:microbiology*; D001938:Brazil; D055323:Byssochlamys / Q000145:classification / Q000302:isolation & purification / Q000502:physiology; D004271:DNA, Fungal; D008969:Molecular Sequence Data; D009758:Nymph / Q000254:growth & development / Q000382:microbiology; D010143:Paecilomyces / Q000145:classification / Q000302:isolation & purification / Q000502:physiology*; D012337:RNA, Ribosomal, 18S; D017422:Sequence Analysis, DNA
chemical_list: D004271:DNA, Fungal; D012337:RNA, Ribosomal, 18S