title: Serological cross-reactivity between Strongyloides venezuelensis and Syphacia muris in Wistar rats (Rattus norvegicus).
abstract: One of the problems frequently faced in laboratory facilities is the possibility of the natural parasitic infection of lab animals, which can interfere with biomedical research results. The present study aimed to evaluate cross-reactivity among serum samples from Wistar rats (Rattus norvegicus) naturally infected with Syphacia muris and experimentally infected with Strongyloides venezuelensis. Forty rats were divided into four groups of ten animals each. Parasite load was evaluated by quantifying the adult worms from both helminthes species recovered from the intestines and the S. venezuelensis eggs eliminated in feces. Serological cross-reactivity by parasite-specific IgG detection was tested via enzyme linked immunosorbent assay (ELISA), immunofluorescence antibody test (IFAT) and immunoblotting. The results demonstrated that the quantity of S. venezuelensis eliminated eggs and parthenogenetic females decreased significantly in cases of co-infection with S. muris. ELISA revealed 100% cross-reactivity of serum samples from both species against the opposing antigen. IgG cross-reactivity was confirmed by IFAT using tissue sections of S. venezuelensis larvae and adult S. muris. Immunoblotting showed that IgG antibodies from the sera of animals infected with S. muris recognized eight antigenic bands from S. venezuelensis saline extract and that IgG antibodies from the sera of animals infected with S. venezuelensis recognized seven bands from S. muris saline extract. These results demonstrate the serological cross-reactivity between S. muris and S. venezuelensis in infected rats.
authors.affiliation: Laboratório de Diagnóstico de Parasitoses, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Av. Amazonas s/n, bloco 4C, Uberlândia, Minas Gerais 38400-902, Brazil.
authors.forename: José Eduardo N
authors.initials: JE
authors.lastname: de Sousa
authors.affiliation: Laboratório de Diagnóstico de Parasitoses, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Av. Amazonas s/n, bloco 4C, Uberlândia, Minas Gerais 38400-902, Brazil.
authors.forename: Edson Fernando G
authors.initials: EF
authors.lastname: de Carvalho
authors.affiliation: Laboratório de Diagnóstico de Parasitoses, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Av. Amazonas s/n, bloco 4C, Uberlândia, Minas Gerais 38400-902, Brazil.
authors.forename: Marcelo A
authors.initials: MA
authors.lastname: Levenhagen
authors.affiliation: Laboratório de Diagnóstico de Parasitoses, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Av. Amazonas s/n, bloco 4C, Uberlândia, Minas Gerais 38400-902, Brazil.
authors.forename: Lucas S
authors.initials: LS
authors.lastname: de Faria
authors.affiliation: Laboratório de Diagnóstico de Parasitoses, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Av. Amazonas s/n, bloco 4C, Uberlândia, Minas Gerais 38400-902, Brazil.
authors.forename: Maria do R F
authors.initials: Mdo R
authors.lastname: Gonçalves-Pires
authors.affiliation: Laboratório de Diagnóstico de Parasitoses, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Av. Amazonas s/n, bloco 4C, Uberlândia, Minas Gerais 38400-902, Brazil. Electronic address: costa-cruz@ufu.br.
authors.forename: Julia M
authors.initials: JM
authors.lastname: Costa-Cruz
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology*; D060085:Coinfection; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000469:parasitology; D005260:Female; D015151:Immunoblotting; D007074:Immunoglobulin G / Q000097:blood*; D007422:Intestines / Q000469:parasitology; D007814:Larva; D010123:Oxyuriasis / Q000150:complications / Q000276:immunology* / Q000469:parasitology / Q000662:veterinary; D010124:Oxyuroidea / Q000276:immunology*; D059208:Parasite Load; D017208:Rats, Wistar; D012698:Serologic Tests; D013321:Strongyloides / Q000276:immunology*; D013322:Strongyloidiasis / Q000150:complications / Q000276:immunology* / Q000469:parasitology
chemical_list: D000947:Antigens, Helminth; D007074:Immunoglobulin G
keywords: Cross-reactivity; Serology; Strongyloides venezuelensis; Syphacia muris