title: The Correlation between Chitin and Acidic Mammalian Chitinase in Animal Models of Allergic Asthma.
abstract: Asthma is the result of chronic inflammation of the airways which subsequently results in airway hyper-responsiveness and airflow obstruction. It has been shown that an elicited expression of acidic mammalian chitinase (AMCase) may be involved in the pathogenesis of asthma. Our recent study has demonstrated that the specific suppression of elevated AMCase leads to reduced eosinophilia and Th2-mediated immune responses in an ovalbumin (OVA)-sensitized mouse model of allergic asthma. In the current study, we show that the elicited expression of AMCase in the lung tissues of both ovalbumin- and Der P2-induced allergic asthma mouse models. The effects of allergic mediated molecules on AMCase expression were evaluated by utilizing promoter assay in the lung cells. In fact, the exposure of chitin, a polymerized sugar and the fundamental component of the major allergen mite and several of the inflammatory mediators, showed significant enhancement on AMCase expression. Such obtained results contribute to the basis of developing a promising therapeutic strategy for asthma by silencing AMCase expression.
authors.affiliation: Department of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, 259 Wen-Hwa 1st Road, Kweishan, Taoyuan 33302, Taiwan. crshen@mail.cgu.edu.tw.|Graduate Institute of Biomedical Science, College of Medicine, Chang Gung University, 259 Wen-Hwa 1st Road, Kweishan, Taoyuan 33302, Taiwan. crshen@mail.cgu.edu.tw.|Chang Gung Immunology Consortium, Chang Gung Memorial Hospital and Chang Gung University, 5 Fu-Hsing Street, Kweishan, Taoyuan 33375, Taiwan. crshen@mail.cgu.edu.tw.
authors.forename: Chia-Rui
authors.initials: CR
authors.lastname: Shen
authors.affiliation: Graduate Institute of Biomedical Science, College of Medicine, Chang Gung University, 259 Wen-Hwa 1st Road, Kweishan, Taoyuan 33302, Taiwan. hhj143@mail.cgu.edu.tw.|Department of Anatomy, College of Medicine, Chang Gung University, 259 Wen-Hwa 1st Road, Kweishan, Taoyuan 33302, Taiwan. hhj143@mail.cgu.edu.tw.
authors.forename: Horng-Heng
authors.initials: HH
authors.lastname: Juang
authors.affiliation: Department of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, 259 Wen-Hwa 1st Road, Kweishan, Taoyuan 33302, Taiwan. m9805024@stmail.cgu.edu.tw.
authors.forename: Hui-Shan
authors.initials: HS
authors.lastname: Chen
authors.affiliation: Department of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, 259 Wen-Hwa 1st Road, Kweishan, Taoyuan 33302, Taiwan. cjyang@mail.dcb.org.tw.|Department of Chemical Engineering, Ming Chi University of Technology, 84 Gung-Juan Road, Taishan, New Taipei 24301, Taiwan. cjyang@mail.dcb.org.tw.
authors.forename: Ching-Jen
authors.initials: CJ
authors.lastname: Yang
authors.affiliation: Graduate Institute of Biomedical Science, College of Medicine, Chang Gung University, 259 Wen-Hwa 1st Road, Kweishan, Taoyuan 33302, Taiwan. popycute@seed.net.tw.|Department of Microbiology, College of Medicine, Chang Gung University, 259 Wen-Hwa 1st Road, Kweishan, Taoyuan 33302, Taiwan. popycute@seed.net.tw.
authors.forename: Chia-Jen
authors.initials: CJ
authors.lastname: Wu
authors.affiliation: Department of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, 259 Wen-Hwa 1st Road, Kweishan, Taoyuan 33302, Taiwan. b9309033@stmail.cgu.edu.tw.|Graduate Institute of Biomedical Science, College of Medicine, Chang Gung University, 259 Wen-Hwa 1st Road, Kweishan, Taoyuan 33302, Taiwan. b9309033@stmail.cgu.edu.tw.|Department of Microbiology, College of Medicine, Chang Gung University, 259 Wen-Hwa 1st Road, Kweishan, Taoyuan 33302, Taiwan. b9309033@stmail.cgu.edu.tw.
authors.forename: Meng-Hua
authors.initials: MH
authors.lastname: Lee
authors.affiliation: Department of Medicine, College of Medicine, Chang Gung University, 259 Wen-Hwa 1st Road, Kweishan, Taoyuan 33302, Taiwan. ejubibi@cgmh.org.tw.|Department of Ophthalmology, Chang Gung Memorial Hospital, 5 Fu-Hsing Street, Kweishan, Taoyuan 33375, Taiwan. ejubibi@cgmh.org.tw.
authors.forename: Yih-Shiou
authors.initials: YS
authors.lastname: Hwang
authors.affiliation: Graduate Institute of Biomedical Science, College of Medicine, Chang Gung University, 259 Wen-Hwa 1st Road, Kweishan, Taoyuan 33302, Taiwan. mingling@mail.cgu.edu.tw.|Chang Gung Immunology Consortium, Chang Gung Memorial Hospital and Chang Gung University, 5 Fu-Hsing Street, Kweishan, Taoyuan 33375, Taiwan. mingling@mail.cgu.edu.tw.|Department of Microbiology, College of Medicine, Chang Gung University, 259 Wen-Hwa 1st Road, Kweishan, Taoyuan 33302, Taiwan. mingling@mail.cgu.edu.tw.
authors.forename: Ming-Ling
authors.initials: ML
authors.lastname: Kuo
authors.affiliation: Department of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, 259 Wen-Hwa 1st Road, Kweishan, Taoyuan 33302, Taiwan. d9601404@stmail.cgu.edu.tw.|Graduate Institute of Biomedical Science, College of Medicine, Chang Gung University, 259 Wen-Hwa 1st Road, Kweishan, Taoyuan 33302, Taiwan. d9601404@stmail.cgu.edu.tw.
authors.forename: Ya-Shan
authors.initials: YS
authors.lastname: Chen
authors.affiliation: Department of Environment and Biotechnology, Refining and Manufacturing Research Institute, CPC Corporation, Chiayi, 217 Min-Sheng S. Rd, Chiayi 60051, Taiwan. 078450@cpc.com.tw.
authors.forename: Jeen-Kuan
authors.initials: JK
authors.lastname: Chen
authors.affiliation: Department of Chemical Engineering, Ming Chi University of Technology, 84 Gung-Juan Road, Taishan, New Taipei 24301, Taiwan. f2402002@ms16.hinet.net.
authors.forename: Chao-Lin
authors.initials: CL
authors.lastname: Liu
mesh_terms: D000485:Allergens / Q000276:immunology; D000818:Animals; D039741:Antigens, Dermatophagoides / Q000276:immunology; D060829:Arthropod Proteins / Q000276:immunology; D001249:Asthma / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism; D002460:Cell Line; D002686:Chitin / Q000494:pharmacology*; D002688:Chitinases / Q000235:genetics* / Q000378:metabolism; D004195:Disease Models, Animal; D005786:Gene Expression Regulation / Q000187:drug effects; D017930:Genes, Reporter; D008168:Lung / Q000276:immunology / Q000378:metabolism; D051379:Mice; D011401:Promoter Regions, Genetic
chemical_list: D000485:Allergens; D039741:Antigens, Dermatophagoides; D060829:Arthropod Proteins; C451163:Dermatophagoides pteronyssinus antigen p 2; D002686:Chitin; D002688:Chitinases
keywords: allergy; asthma; chitinase; promoter