title: Collagen Induced Arthritis in DBA/1J Mice Associates with Oxylipin Changes in Plasma.
abstract: Oxylipins play important roles in various biological processes and are considered as mediators of inflammation for a wide range of diseases such as rheumatoid arthritis (RA). The purpose of this research was to study differences in oxylipin levels between a widely used collagen induced arthritis (CIA) mice model and healthy control (Ctrl) mice. DBA/1J male mice (age: 6-7 weeks) were selected and randomly divided into two groups, namely, a CIA and a Ctrl group. The CIA mice were injected intraperitoneally (i.p.) with the joint cartilage component collagen type II (CII) and an adjuvant injection of lipopolysaccharide (LPS). Oxylipin metabolites were extracted from plasma for each individual sample using solid phase extraction (SPE) and were detected with high performance liquid chromatography/tandem mass spectrometry (HPLC-ESI-MS/MS), using dynamic multiple reaction monitoring (dMRM). Both univariate and multivariate statistical analyses were applied. The results in univariate Student's t-test revealed 10 significantly up- or downregulated oxylipins in CIA mice, which were supplemented by another 6 additional oxylipins, contributing to group clustering upon multivariate analysis. The dysregulation of these oxylipins revealed the presence of ROS-generated oxylipins and an increase of inflammation in CIA mice. The results also suggested that the collagen induced arthritis might associate with dysregulation of apoptosis, possibly inhibited by activated NF-κB because of insufficient PPAR-γ ligands.
authors.affiliation: Analytical BioSciences, LACDR, Leiden University, P.O. Box 9502, 2300 RA Leiden, Netherlands ; Sino-Dutch Centre for Preventive and Personalized Medicine, Leiden University, P.O. Box 9502, 2300 RA Leiden, Netherlands.
authors.forename: Min
authors.initials: M
authors.lastname: He
authors.affiliation: Analytical BioSciences, LACDR, Leiden University, P.O. Box 9502, 2300 RA Leiden, Netherlands ; Sino-Dutch Centre for Preventive and Personalized Medicine, Leiden University, P.O. Box 9502, 2300 RA Leiden, Netherlands.
authors.forename: Eduard
authors.initials: E
authors.lastname: van Wijk
authors.affiliation: Analytical BioSciences, LACDR, Leiden University, P.O. Box 9502, 2300 RA Leiden, Netherlands.
authors.forename: Ruud
authors.initials: R
authors.lastname: Berger
authors.affiliation: Analytical BioSciences, LACDR, Leiden University, P.O. Box 9502, 2300 RA Leiden, Netherlands ; Sino-Dutch Centre for Preventive and Personalized Medicine, Leiden University, P.O. Box 9502, 2300 RA Leiden, Netherlands ; SU Biomedicine, Utrechtseweg 48, 3700 AJ Zeist, Netherlands.
authors.forename: Mei
authors.initials: M
authors.lastname: Wang
authors.affiliation: Analytical BioSciences, LACDR, Leiden University, P.O. Box 9502, 2300 RA Leiden, Netherlands.
authors.forename: Katrin
authors.initials: K
authors.lastname: Strassburg
authors.affiliation: Analytical BioSciences, LACDR, Leiden University, P.O. Box 9502, 2300 RA Leiden, Netherlands.
authors.forename: Johannes C
authors.initials: JC
authors.lastname: Schoeman
authors.affiliation: Analytical BioSciences, LACDR, Leiden University, P.O. Box 9502, 2300 RA Leiden, Netherlands ; Discovery Sciences, Janssen Research & Development, Turnhoutseweg 30, 2340 Beerse, Belgium.
authors.forename: Rob J
authors.initials: RJ
authors.lastname: Vreeken
authors.affiliation: Analytical BioSciences, LACDR, Leiden University, P.O. Box 9502, 2300 RA Leiden, Netherlands ; Sino-Dutch Centre for Preventive and Personalized Medicine, Leiden University, P.O. Box 9502, 2300 RA Leiden, Netherlands ; TNO Innovation for Life, P.O. Box 360, 3700 AJ Zeist, Netherlands.
authors.forename: Herman
authors.initials: H
authors.lastname: van Wietmarschen
authors.affiliation: Analytical BioSciences, LACDR, Leiden University, P.O. Box 9502, 2300 RA Leiden, Netherlands.
authors.forename: Amy C
authors.initials: AC
authors.lastname: Harms
authors.affiliation: Tohoku Institute of Technology, Sendai 982-8577, Japan.
authors.forename: Masaki
authors.initials: M
authors.lastname: Kobayashi
authors.affiliation: Analytical BioSciences, LACDR, Leiden University, P.O. Box 9502, 2300 RA Leiden, Netherlands ; Sino-Dutch Centre for Preventive and Personalized Medicine, Leiden University, P.O. Box 9502, 2300 RA Leiden, Netherlands.
authors.forename: Thomas
authors.initials: T
authors.lastname: Hankemeier
authors.affiliation: Analytical BioSciences, LACDR, Leiden University, P.O. Box 9502, 2300 RA Leiden, Netherlands ; Sino-Dutch Centre for Preventive and Personalized Medicine, Leiden University, P.O. Box 9502, 2300 RA Leiden, Netherlands ; TNO Innovation for Life, P.O. Box 360, 3700 AJ Zeist, Netherlands.
authors.forename: Jan
authors.initials: J
authors.lastname: van der Greef
mesh_terms: D000818:Animals; D001169:Arthritis, Experimental / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D008297:Male; D051379:Mice; D008811:Mice, Inbred DBA; D016328:NF-kappa B / Q000502:physiology; D054883:Oxylipins / Q000097:blood*; D017382:Reactive Oxygen Species / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
chemical_list: D016328:NF-kappa B; D054883:Oxylipins; D017382:Reactive Oxygen Species