title: Cholesterol Flux Is Required for Endosomal Progression of African Swine Fever Virions during the Initial Establishment of Infection.
abstract: African swine fever virus (ASFV) is a major threat for porcine production that has been slowly spreading in Eastern Europe since its first appearance in the Caucasus in 2007. ASFV enters the cell by endocytosis and gains access to the cytosol to start replication from late endosomes and multivesicular bodies. Cholesterol associated with low-density lipoproteins entering the cell by endocytosis also follows a trafficking pathway similar to that of ASFV. Here we show that cholesterol plays an essential role in the establishment of infection as the virus traffics through the endocytic pathway. In contrast to the case for other DNA viruses, such as vaccinia virus or adenovirus 5, cholesterol efflux from endosomes is required for ASFV release/entry to the cytosol. Accumulation of cholesterol in endosomes impairs fusion, resulting in retention of virions inside endosomes. ASFV also remodels intracellular cholesterol by increasing its cellular uptake and redistributes free cholesterol to viral replication sites. Our analysis reveals that ASFV manipulates cholesterol dynamics to ensure an appropriate lipid flux to establish productive infection.
  
  
  OBJECTIVE
  Since its appearance in the Caucasus in 2007, African swine fever (ASF) has been spreading westwards to neighboring European countries, threatening porcine production. Due to the lack of an effective vaccine, ASF control relies on early diagnosis and widespread culling of infected animals. We investigated early stages of ASFV infection to identify potential cellular targets for therapeutic intervention against ASF. The virus enters the cell by endocytosis, and soon thereafter, viral decapsidation occurs in the acid pH of late endosomes. We found that ASFV infection requires and reorganizes the cellular lipid cholesterol. ASFV requires cholesterol to exit the endosome to gain access to the cytoplasm to establish productive replication. Our results indicate that there is a differential requirement for cholesterol efflux for vaccinia virus or adenovirus 5 compared to ASFV.
authors.affiliation: Department of Biotechnology, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Madrid, Spain.
authors.forename: Miguel Ángel
authors.initials: MÁ
authors.lastname: Cuesta-Geijo
authors.affiliation: Department of Structure of Macromolecules, Centro Nacional de Biotecnología, Universidad Autónoma de Madrid, Madrid, Spain.
authors.forename: Michele
authors.initials: M
authors.lastname: Chiappi
authors.affiliation: Department of Biotechnology, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Madrid, Spain.
authors.forename: Inmaculada
authors.initials: I
authors.lastname: Galindo
authors.affiliation: Department of Biotechnology, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Madrid, Spain.
authors.forename: Lucía
authors.initials: L
authors.lastname: Barrado-Gil
authors.affiliation: Department of Biotechnology, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Madrid, Spain.
authors.forename: Raquel
authors.initials: R
authors.lastname: Muñoz-Moreno
authors.affiliation: Department of Structure of Macromolecules, Centro Nacional de Biotecnología, Universidad Autónoma de Madrid, Madrid, Spain.
authors.forename: José L
authors.initials: JL
authors.lastname: Carrascosa
authors.affiliation: Department of Biotechnology, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Madrid, Spain calonso@inia.es.
authors.forename: Covadonga
authors.identifier: http://orcid.org/0000-0002-0862-6177
authors.initials: C
authors.lastname: Alonso
mesh_terms: D000358:African Swine Fever Virus / Q000502:physiology*; D000818:Animals; D002522:Chlorocebus aethiops; D002784:Cholesterol / Q000378:metabolism*; D011992:Endosomes / Q000378:metabolism* / Q000821:virology*; D006863:Hydrogen-Ion Concentration; D064688:Metabolic Flux Analysis; D014709:Vero Cells; D053586:Virus Internalization
chemical_list: D002784:Cholesterol