title: Cytotoxic and anti-inflammatory activities of phenanthrenes from the medullae of Juncus effusus L.
abstract: Bioactivity guided phytochemical investigation of the ethanol extract of the medullae of Juncus effusus resulted in the isolation of two new phenanthrenes, 8-hydroxymethyl-2-hydroxyl-1-methyl-5-vinyl-9,10-dihydrophenanthrene (1), and 5-(1-methoxyethyl)-1-methyl-phenanthren-2,7-diol (2) together with 15 known phenanthrenoids (3-17). The chemical structures of 1 and 2 were established by a combination of spectroscopic techniques. Compounds 1-15 and 17 were evaluated for their cytotoxic activities against five human cancer cell lines (SHSY-5Y, SMMC-7721, HepG-2, Hela and MCF-7) by CCK-8 assay, and their anti-inflammatory activities were also evaluated by inhibition on NO production in LPS-activated murine macrophage RAW 264.7 cells.
authors.affiliation: School of Pharmacy, Anhui Medical University, 81 Meishan Road, Hefei, 230032, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Ma
authors.affiliation: School of Pharmacy, Anhui Medical University, 81 Meishan Road, Hefei, 230032, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: School of Pharmacy, Anhui Medical University, 81 Meishan Road, Hefei, 230032, China.
authors.forename: Yun-Yun
authors.initials: YY
authors.lastname: Ding
authors.affiliation: School of Pharmacy, Anhui Medical University, 81 Meishan Road, Hefei, 230032, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Liu
authors.affiliation: School of Pharmacy, Anhui Medical University, 81 Meishan Road, Hefei, 230032, China. ln0110@sina.com.
authors.forename: Ning
authors.initials: N
authors.lastname: Li
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D006367:HeLa Cells; D056945:Hep G2 Cells; D006801:Humans; D020128:Inhibitory Concentration 50; D061986:MCF-7 Cells; D008262:Macrophage Activation / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D019684:Magnoliopsida / Q000737:chemistry*; D051379:Mice; D015394:Molecular Structure; D009369:Neoplasms / Q000188:drug therapy* / Q000473:pathology; D009569:Nitric Oxide / Q000378:metabolism; D010616:Phenanthrenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal; D000067996:RAW 264.7 Cells; D013329:Structure-Activity Relationship
chemical_list: D000893:Anti-Inflammatory Agents; D000972:Antineoplastic Agents, Phytogenic; D010616:Phenanthrenes; D010936:Plant Extracts; D009569:Nitric Oxide
keywords: Anti-inflammatory activity; Cytotoxicity; Juncaceae; Juncus effuses; Phenanthrene