title: Cerebrospinal fluid from rats given hypoxic preconditioning protects neurons from oxygen-glucose deprivation-induced injury.
abstract: Hypoxic preconditioning activates endogenous mechanisms that protect against cerebral ischemic and hypoxic injury. To better understand these protective mechanisms, adult rats were housed in a hypoxic environment (8% O2/92% N2) for 3 hours, and then in a normal oxygen environment for 12 hours. Their cerebrospinal fluid was obtained to culture cortical neurons from newborn rats for 1 day, and then the neurons were exposed to oxygen-glucose deprivation for 1.5 hours. The cerebrospinal fluid from rats subjected to hypoxic preconditioning reduced oxygen-glucose deprivation-induced injury, increased survival rate, upregulated Bcl-2 expression and downregulated Bax expression in the cultured cortical neurons, compared with control. These results indicate that cerebrospinal fluid from rats given hypoxic preconditioning protects against oxygen-glucose deprivation-induced injury by affecting apoptosis-related protein expression in neurons from newborn rats.
authors.affiliation: Department of Neurology, Affiliated Hospital of Taishan Medical University, Taian, Shandong Province, China.
authors.forename: Yan-Bo
authors.identifier: 0000-0002-0829-9815
authors.initials: YB
authors.lastname: Zhang
authors.affiliation: Department of Endocrinology, Affiliated Hospital of Taishan Medical University, Taian, Shandong Province, China.
authors.forename: Zheng-Dong
authors.initials: ZD
authors.lastname: Guo
authors.affiliation: Department of Neurology, Shandong Taishan Chronic Disease Hospital, Taian, Shandong Province, China.
authors.forename: Mei-Yi
authors.initials: MY
authors.lastname: Li
authors.affiliation: Hypoxia Medical Institute, Xuanwu Hospital, Capital Medical University, Beijing, China.
authors.forename: Si-Jie
authors.initials: SJ
authors.lastname: Li
authors.affiliation: Department of Neurology, Affiliated Hospital of Taishan Medical University, Taian, Shandong Province, China.
authors.forename: Jing-Zhong
authors.initials: JZ
authors.lastname: Niu
authors.affiliation: Department of Neurology, Affiliated Hospital of Taishan Medical University, Taian, Shandong Province, China.
authors.forename: Ming-Feng
authors.initials: MF
authors.lastname: Yang
authors.affiliation: Hypoxia Medical Institute, Xuanwu Hospital, Capital Medical University, Beijing, China.
authors.forename: Xun-Ming
authors.initials: XM
authors.lastname: Ji
authors.affiliation: Hypoxia Medical Institute, Xuanwu Hospital, Capital Medical University, Beijing, China.
authors.forename: Guo-Wei
authors.initials: GW
authors.lastname: Lv
keywords: Bcl-2/Bax; apoptosis; cerebral cortex; cerebrospinal fluid; hypoxic preconditioning; nerve regeneration; neural regeneration; neurons; oxygen-glucose deprivation