title: SPO73 and SPO71 Function Cooperatively in Prospore Membrane Elongation During Sporulation in Saccharomyces cerevisiae.
abstract: In the yeast Saccharomyces cerevisiae, cells undergoing sporulation form prospore membranes to surround their meiotic nuclei. The prospore membranes ultimately become the plasma membranes of the new cells. The putative phospholipase Spo1 and the tandem Pleckstrin Homology domain protein Spo71 have previously been shown to be required for prospore membrane development, along with the constitutively expressed Vps13 involved in vacuolar sorting. Here, we utilize genetic analysis, and find that SPO73 is required for proper prospore membrane shape and, like SPO71, is necessary for prospore membrane elongation. Additionally, similar to SPO71, loss of SPO73 partially suppresses spo1Δ. Spo73 localizes to prospore membranes and complexes with Spo71. We also find that phosphatidylserine localizes to the prospore membrane. Our results suggest a model where SPO71 and SPO73 act in opposition to SPO1 to form and elongate prospore membranes, while VPS13 plays a distinct role in prospore membrane development.
authors.affiliation: Department of Biology, University of Massachusetts Boston, Boston, Massachusetts, United States of America.
authors.forename: Emily M
authors.initials: EM
authors.lastname: Parodi
authors.affiliation: Department of Biology, University of Massachusetts Boston, Boston, Massachusetts, United States of America.
authors.forename: Joseph M
authors.initials: JM
authors.lastname: Roesner
authors.affiliation: Department of Biology, University of Massachusetts Boston, Boston, Massachusetts, United States of America.
authors.forename: Linda S
authors.initials: LS
authors.lastname: Huang
mesh_terms: D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism*; D055785:Gene Knockdown Techniques; D008245:Lysophospholipase / Q000235:genetics / Q000378:metabolism; D046912:Multiprotein Complexes / Q000378:metabolism; D009154:Mutation; D010641:Phenotype; D010718:Phosphatidylserines / Q000378:metabolism; D011485:Protein Binding; D021381:Protein Transport; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D013172:Spores, Fungal
chemical_list: D002352:Carrier Proteins; D046912:Multiprotein Complexes; D010718:Phosphatidylserines; C578370:SPO71 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; C110276:VPS13 protein, S cerevisiae; D008245:Lysophospholipase; C102293:SPO1 protein, S cerevisiae