title: Sensitive and Rapid UHPLC-MS/MS for the Analysis of Tomato Phenolics in Human Biological Samples.
abstract: An UHPLC-MS/MS method for the quantification of tomato phenolic metabolites in human fluids was optimized and validated, and then applied in a pilot dietary intervention study with healthy volunteers. A 5-fold gain in speed (3.5 min of total run); 7-fold increase in MS sensitivity and 2-fold greater efficiency (50% peak width reduction) were observed when comparing the proposed method with the reference-quality HPLC-MS/MS system, whose assay performance has been previously documented. The UHPLC-MS/MS method led to an overall improvement in the limits of detection (LOD) and quantification (LOQ) for all the phenolic compounds studied. The recoveries ranged between 68% and 100% in urine and 61% and 100% in plasma. The accuracy; intra- and interday precision; and stability met with the acceptance criteria of the AOAC International norms. Due to the improvements in the analytical method; the total phenolic metabolites detected in plasma and urine in the pilot intervention study were 3 times higher than those detected by HPLC-MS/MS. Comparing with traditional methods; which require longer time of analysis; the methodology described is suitable for the analysis of phenolic compounds in a large number of plasma and urine samples in a reduced time frame.
authors.affiliation: Department of Nutrition and Food Science-XARTA-INSA, School of Pharmacy, University of Barcelona, Barcelona 08028, Spain. mmartinezh@ub.edu.|Centre for Biomedical Network Research on the Pathophysiology of Obesity and Nutrition (CIBEROBN). Carlos III Health Institute, Madrid 28029, Spain. mmartinezh@ub.edu.
authors.forename: Miriam
authors.initials: M
authors.lastname: Martínez-Huélamo
authors.affiliation: Biomedical Research Institute (IBIMA), Service of Endocrinology and Nutrition, Hospital Virgen de la Victoria, Teatinos Campus, University of Malaga, Malaga 29010, Spain. sara.tulipani@gmail.com.
authors.forename: Sara
authors.initials: S
authors.lastname: Tulipani
authors.affiliation: Scientific and Technological Centers of the University of Barcelona (CCiTUB), Barcelona 08028, Spain. ojauregui@ccit.ub.edu.
authors.forename: Olga
authors.initials: O
authors.lastname: Jáuregui
authors.affiliation: Centre for Biomedical Network Research on the Pathophysiology of Obesity and Nutrition (CIBEROBN). Carlos III Health Institute, Madrid 28029, Spain. palmi.vm@gmail.com.|Department of Internal Medicine, Hospital Clinic, Institute of Biomedical Investigation August Pi i Sunyer (IDIBAPS), University of Barcelona, Barcelona 08036, Spain. palmi.vm@gmail.com.
authors.forename: Palmira
authors.initials: P
authors.lastname: Valderas-Martinez
authors.affiliation: Centre for Biomedical Network Research on the Pathophysiology of Obesity and Nutrition (CIBEROBN). Carlos III Health Institute, Madrid 28029, Spain. avallverdu@ub.edu.|INRA, UMR1083 Sciences for Oenology, 2 place Pierre Viala, Montpellier Cedex 34060, France. avallverdu@ub.edu.
authors.forename: Anna
authors.initials: A
authors.lastname: Vallverdú-Queralt
authors.affiliation: Centre for Biomedical Network Research on the Pathophysiology of Obesity and Nutrition (CIBEROBN). Carlos III Health Institute, Madrid 28029, Spain. restruch@clinic.ub.es.|Department of Internal Medicine, Hospital Clinic, Institute of Biomedical Investigation August Pi i Sunyer (IDIBAPS), University of Barcelona, Barcelona 08036, Spain. restruch@clinic.ub.es.
authors.forename: Ramón
authors.initials: R
authors.lastname: Estruch
authors.affiliation: Department of Nutrition and Food Science-XARTA-INSA, School of Pharmacy, University of Barcelona, Barcelona 08028, Spain. xaviertorrado@ub.edu.
authors.forename: Xavier
authors.initials: X
authors.lastname: Torrado
authors.affiliation: Department of Nutrition and Food Science-XARTA-INSA, School of Pharmacy, University of Barcelona, Barcelona 08028, Spain. lamuela@ub.edu.|Centre for Biomedical Network Research on the Pathophysiology of Obesity and Nutrition (CIBEROBN). Carlos III Health Institute, Madrid 28029, Spain. lamuela@ub.edu.
authors.forename: Rosa M
authors.initials: RM
authors.lastname: Lamuela-Raventós
mesh_terms: D000328:Adult; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D057230:Limit of Detection; D018551:Solanum lycopersicum / Q000737:chemistry*; D010636:Phenols / Q000737:chemistry* / Q000493:pharmacokinetics*; D053147:Plant Exudates / Q000737:chemistry* / Q000493:pharmacokinetics*; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry; D055815:Young Adult
chemical_list: D010636:Phenols; D053147:Plant Exudates
keywords: biosamples; blood; metabolites; microbiota; polyphenol metabolites; sauce; tomato; urine; validation