title: Increased expression of circulating miRNA-93 in women with polycystic ovary syndrome may represent a novel, non-invasive biomarker for diagnosis.
abstract: MicroRNAs (miRNA) are a novel class of small noncoding single-stranded RNA molecules that regulate gene expression. There is increasing evidence of their importance in polycystic ovary syndrome (PCOS). The objective was to determine if miRNA-93 and miRNA-223 are differentially expressed in the circulation of women with PCOS compared to age matched women. A case-control study comparing women with PCOS (n = 25) to age and weight matched controls (n = 24) without PCOS was performed. MiRNA-93 and miRNA-223 were determined by total RNA reverse transcription. Both miRNA-93 and miRNA-223 were significantly increased relative to the control group (p < 0.01, p = 0.029 respectively). In both groups there was no correlation of either miRNA-93 or miRNA-223 with insulin, HOMA-IR, HOMA-β or testosterone levels. The area under the receiver operator characteristic curve for miR-223 and miR-93 was 0.66 and 0.72 respectively, suggesting miR-93 is a more efficient biomarker than miR-223 for diagnosis of PCOS. The combination of the two miRNAs together, tested using multiple logistic regression analysis, did not improve the diagnostic potential. In conclusion, circulating miRNA-93 and miRNA-223 were higher in women with PCOS compared to age and weight matched controls independent of insulin resistance and testosterone levels, and miR-93 may represent a novel diagnostic biomarker for PCOS.
authors.affiliation: Academic Diabetes, Endocrinology and Metabolism, Hull York Medical School, University of Hull, UK.|Faculty of Medicine, Department of Surgery &Cancer, Imperial College, London, UK.
authors.forename: T
authors.initials: T
authors.lastname: Sathyapalan
authors.affiliation: Faculty of Medicine, Department of Surgery &Cancer, Imperial College, London, UK.
authors.forename: R
authors.initials: R
authors.lastname: David
authors.affiliation: Faculty of Medicine, Department of Surgery &Cancer, Imperial College, London, UK.
authors.forename: N J
authors.initials: NJ
authors.lastname: Gooderham
authors.affiliation: Weill Cornell Medical College Qatar, Education City PO Box 24144, Doha, Qatar.
authors.forename: S L
authors.initials: SL
authors.lastname: Atkin
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D000328:Adult; D014408:Biomarkers, Tumor / Q000097:blood* / Q000235:genetics* / Q000378:metabolism; D015992:Body Mass Index; D016022:Case-Control Studies; D003710:Demography; D005260:Female; D006801:Humans; D035683:MicroRNAs / Q000097:blood* / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D011085:Polycystic Ovary Syndrome / Q000097:blood* / Q000175:diagnosis / Q000235:genetics*; D012372:ROC Curve; D012984:Software; D055815:Young Adult
chemical_list: D020413:3' Untranslated Regions; D014408:Biomarkers, Tumor; D035683:MicroRNAs; C525067:MIR223, human; C543350:MIRN93 microRNA, human