title: [Differential expressions of microRNAs and their predicted targets in liver transplant recipients with long-term stable survival].
abstract: OBJECTIVE
  To determine whether the miRNA expression profile in peripheral blood mononuclear cells (PBMCs) differs between liver transplant recipients with long-term stable survival and those with acute rejection.
  
  
  METHODS
  Twenty-nine liver transplant recipients with long-term stable survival (STA) group, 10 recipients with acute rejection (RJ group), and 17 healthy subjects (control group) were recruited for genome-wide microarray analysis of miRNA expressions in the PBMCs. The differentially expressed miRNAs among the 3 groups were validated by real-time PCR, and the targets of these miRNAs were predicted.
  
  
  RESULTS
  Compared with the RJ group, the STA group showed down-regulation of 13 miRNAs in the PBMCs. Of these down-regulated miRNAs, miRNA-18b, miRNA-340 and miRNA-106b were validated by real-time PCR, and the latter two miRNAs were predicted to target the TGF-β pathway.
  
  
  CONCLUSIONS
  The differentially expressed miRNAs in liver transplant recipients with long-term stable survival, namely miRNA-18b, miRNA-340 and miRNA-106b, can be potential clinical biomarkers to predict the outcomes of liver transplantation.
authors.affiliation: Department of Transplantation Center, First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China.E-mail: zhong_kb@126.com.
authors.forename: Kebo
authors.initials: K
authors.lastname: Zhong
authors.forename: Peng
authors.initials: P
authors.lastname: Zhang
authors.forename: Xiaoshun
authors.initials: X
authors.lastname: He
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Zhu
mesh_terms: D015415:Biomarkers / Q000378:metabolism; D016022:Case-Control Studies; D015536:Down-Regulation; D006084:Graft Rejection; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D016031:Liver Transplantation; D035683:MicroRNAs / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D060888:Real-Time Polymerase Chain Reaction; D015996:Survival Rate; D016212:Transforming Growth Factor beta
chemical_list: D015415:Biomarkers; C542541:MIRN106 microRNA, human; C581632:MIRN18 microRNA, human; C570046:MIRN340 microRNA, human; D035683:MicroRNAs; D016212:Transforming Growth Factor beta