title: IL-9 antibody injection suppresses the inflammation in colitis mice.
abstract: Diverse T help (Th) cells play a crucial role in the processing and maintaining of chronic inflammation as seen in ulcerative colitis (UC). Th9, a novel subset of Th cells that primarily produces interleukin (IL)-9, has recently been associated with the development of inflammatory diseases. In this study, we evaluated the presentation of Th9 cells in inflamed tissues of human and experimental mouse UC, and examined the therapeutic efficiency of anti Th9 cytokine IL-9 in the experimental mouse UC. Using immunohistochemistry (IHC), we evaluated the presentation of Th9 cells labelled by transcriptional factor PU.1 in both human and dextran sulfate sodium (DSS) induced mouse colitis biopsies. The results showed that increased PU.1 positive Th9 cells were mainly located in the lamina propria in relative with the controls, intraepithelial Th9 cells can also be observed but at low density. Double IHCs revealed that most of PU.1 positive cells were CD3 positive lymphocytes in human UC specimens. Anti-IL-9 antibody injection for 2 weeks reduced the severity of inflammation in DSS induced colitis mice. Our results suggest that The Th9/IL-9 is involved in the pathogenesis of UC.
authors.affiliation: Laboratory of Molecular Cell Biology, Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, Ås, Norway; Research Group of Gastrointestinal Diseases, The Second Affiliated Hospital of Zhengzhou University, Henan, China.
authors.forename: Aping
authors.initials: A
authors.lastname: Yuan
authors.affiliation: Research Group of Gastrointestinal Diseases, The Second Affiliated Hospital of Zhengzhou University, Henan, China.
authors.forename: Hang
authors.initials: H
authors.lastname: Yang
authors.affiliation: Research Group of Gastrointestinal Diseases, The Second Affiliated Hospital of Zhengzhou University, Henan, China.
authors.forename: Haili
authors.initials: H
authors.lastname: Qi
authors.affiliation: Research Group of Gastrointestinal Diseases, The Second Affiliated Hospital of Zhengzhou University, Henan, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Cui
authors.affiliation: Research Group of Gastrointestinal Diseases, The Second Affiliated Hospital of Zhengzhou University, Henan, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Hua
authors.affiliation: Research Group of Gastrointestinal Diseases, The Second Affiliated Hospital of Zhengzhou University, Henan, China.
authors.forename: Can
authors.initials: C
authors.lastname: Li
authors.affiliation: Research Group of Gastrointestinal Diseases, The Second Affiliated Hospital of Zhengzhou University, Henan, China.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Pang
authors.affiliation: Research Group of Gastrointestinal Diseases, The Second Affiliated Hospital of Zhengzhou University, Henan, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zheng
authors.affiliation: Research Group of Gastrointestinal Diseases, The Second Affiliated Hospital of Zhengzhou University, Henan, China; Faculty of Health, Nord University at Levanger, Norway. Electronic address: guanglin.cui@yahoo.com.
authors.forename: Guanglin
authors.initials: G
authors.lastname: Cui
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage; D000911:Antibodies, Monoclonal / Q000008:administration & dosage*; D002460:Cell Line; D003092:Colitis / Q000276:immunology* / Q000517:prevention & control*; D003106:Colon / Q000187:drug effects / Q000276:immunology*; D005260:Female; D006801:Humans; D007267:Injections; D007274:Injections, Intraperitoneal; D016906:Interleukin-9 / Q000037:antagonists & inhibitors / Q000276:immunology*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008875:Middle Aged; D006377:T-Lymphocytes, Helper-Inducer / Q000187:drug effects / Q000276:immunology*; D016896:Treatment Outcome
chemical_list: D000893:Anti-Inflammatory Agents; D000911:Antibodies, Monoclonal; D016906:Interleukin-9
keywords: IL-9; Inflammation; Th9; Ulcerative colitis