title: Effect of Circadian Rhythm Disruption and Alcohol on the Oxidative Stress Level in Rat Brain.
abstract: Alcohol abuse is often associated with disrupted circadian rhythms and sleep, and the link seems to be bidirectional. In addition, it has been shown that exposure to constant illumination may increase lipid peroxidation in tissues. In this study, we investigated the effects of circadian rhythm disruption (CRD) and chronic alcohol intake (A) alone and in combination (CRD+A), on the oxidative stress in total rat brain homogenate. Our results demonstrated that lipid peroxidation was increased in the brain samples of all experimental animals compared with the control ones. The oxidative stress levels increased in the order: C<CRD<A<(CRD+A). Furthermore, xanthine oxidase activity changed in the order: C<CRD<A=CRD+A. It was proposed that chronic alcohol intake, if accompanied with CRD, might lead to significantly higher lipid peroxidation in the brain, than if present alone. Our data suggest that ethanol metabolites may be involved in the increase of the brain xanthine oxidase activity in rats with chronic alcohol intake.
authors.affiliation: Departments of 1Pharmacology and Toxicology; and 2Medical Physics and Biophysics, Medical Faculty, Medical University, Sofia, Bulgaria.
authors.forename: Miroslava Georgieva
authors.initials: MG
authors.lastname: Varadinova
authors.forename: Maria Lozanova
authors.initials: ML
authors.lastname: Valcheva-Traykova
authors.forename: Nadka Ivanova
authors.initials: NI
authors.lastname: Boyadjieva
mesh_terms: D000428:Alcohol Drinking / Q000009:adverse effects; D000437:Alcoholism / Q000150:complications*; D000818:Animals; D001921:Brain / Q000378:metabolism / Q000473:pathology; D021081:Chronobiology Disorders / Q000503:physiopathology*; D002940:Circadian Rhythm / Q000502:physiology; D004195:Disease Models, Animal; D000431:Ethanol / Q000008:administration & dosage / Q000378:metabolism / Q000633:toxicity*; D015227:Lipid Peroxidation / Q000502:physiology; D008297:Male; D018384:Oxidative Stress / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D014969:Xanthine Oxidase / Q000378:metabolism
chemical_list: D000431:Ethanol; D014969:Xanthine Oxidase