title: Colorimetric Detection of Some Highly Hydrophobic Flavonoids Using Polydiacetylene Liposomes Containing Pentacosa-10,12-diynoyl Succinoglycan Monomers.
abstract: Flavonoids are a group of plant secondary metabolites including polyphenolic molecules, and they are well known for antioxidant, anti-allergic, anti-inflammatory and anti-viral propertied. In general, flavonoids are detected with various non-colorimetric detection methods such as column liquid chromatography, thin-layer chromatography, and electrochemical analysis. For the first time, we developed a straightforward colorimetric detection system allowing recognition of some highly hydrophobic flavonoids such as alpha-naphthoflavone and beta-naphthoflavone, visually using 10,12-pentacosadiynoic acid (PCDA) derivatized with succinoglycan monomers isolated from Sinorhizobium meliloti. Besides changes in visible spectrum, we also demonstrate fluorescence changes using our detection system in the presence of those flavonoids. The succinoglycan monomers attached to PCDA molecules may function as an unstructured molecular capturer for some highly hydrophobic flavonoids by hydrophobic interactions, and transmit their molecular interactions as a color change throughout the PCDA liposome.
authors.affiliation: Department of Bioscience and Biotechnology, Microbial Carbohydrate Resource Bank (MCRB) & Center for Biotechnology Research in UBITA (CBRU), Konkuk University, Seoul, South Korea.
authors.forename: Deokgyu
authors.initials: D
authors.lastname: Yun
authors.affiliation: Department of Bioscience and Biotechnology, Microbial Carbohydrate Resource Bank (MCRB) & Center for Biotechnology Research in UBITA (CBRU), Konkuk University, Seoul, South Korea.
authors.forename: Daham
authors.initials: D
authors.lastname: Jeong
authors.affiliation: Institute for Ubiquitous Information Technology and Applications (UBITA) & Center for Biotechnology Research in UBITA (CBRU), Konkuk University, Seoul, South Korea.
authors.forename: Eunae
authors.initials: E
authors.lastname: Cho
authors.affiliation: Department of Bioscience and Biotechnology, Microbial Carbohydrate Resource Bank (MCRB) & Center for Biotechnology Research in UBITA (CBRU), Konkuk University, Seoul, South Korea.
authors.forename: Seunho
authors.initials: S
authors.lastname: Jung
mesh_terms: D003124:Colorimetry / Q000379:methods*; D005231:Fatty Acids, Unsaturated / Q000737:chemistry; D005419:Flavonoids / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D008081:Liposomes / Q000737:chemistry*; D000078789:Polyacetylene Polymer; D011108:Polymers / Q000737:chemistry*; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D053279:Polyynes / Q000737:chemistry*
chemical_list: C493048:10, 12-pentacosadiynoic acid; D005231:Fatty Acids, Unsaturated; D005419:Flavonoids; D008081:Liposomes; D000078789:Polyacetylene Polymer; D011108:Polymers; D011135:Polysaccharides, Bacterial; D053279:Polyynes; C082361:polydiacetylene; C056779:succinoglycan