title: Dual effects of heme oxygenase-1 on astrocyte injury induced by hemin in vitro.
abstract: OBJECTIVE
  The purpose of this study was to investigate the effects of heme oxygenase-1 (HO-1) on astrocyte injury induced by hemin.
  
  
  METHODS
  Primary astrocytes were isolated from Sprague Dawley rat pups and cultured in vitro. The expression of HO-1 was induced by hemin in a quantitative fashion and the effects of HO-1 on hemin-induced astrocyte injury were estimated by cell viability, cell membrane permeability and apoptosis.
  
  
  METHODS
  Astrocytes were divided into control group, hemin 5 μM group, hemin 5 μM + Zn-PPIX group, hemin 30 μM group and hemin 30 μM + Zn-PPIX group. Survival quality of astrocyte was measured by WST-8 assay, LDH assay, Hoechst 33258 Staining and annexin V-FITC/PI assay and apoptotic-related proteins were measured using Western blotting.
  
  
  RESULTS
  Hemin could dose-dependently up-regulate the expression of HO-1. HO-1 exerted a protective role on astrocyte damage induced by 5 μM hemin, including increased cell survival rate and anti-apoptotic proteins expression (Bcl-2 and p-AKT), as well as decreased LDH release, apoptosis ratio and apoptotic protein expression (Bax, p-ERK and cleaved-caspase3). However, the effect of HO-1 on astrocyte injury between 30 μM hemin-treated groups was opposite of the protective role in 5 μM hemin-treated groups.
  
  
  CONCLUSIONS
  There were dual effects of HO-1 in 5 μM and 30 μM hemin-induced astrocyte injuries.
authors.affiliation: a Department of Neurology , Affiliated Hospital of Xuzhou Medical College , Xuzhou, Jiangsu Province , P.R. China .
authors.forename: Hao
authors.initials: H
authors.lastname: Chen
authors.affiliation: b School of Life Science , Nanjing , Jiangsu Province , P.R. China , and.
authors.forename: Xingqi
authors.initials: X
authors.lastname: Wang
authors.affiliation: c Medical School, Nanjing University , Nanjing , Jiangsu Province , P.R. China.
authors.forename: Qiuchen
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: a Department of Neurology , Affiliated Hospital of Xuzhou Medical College , Xuzhou, Jiangsu Province , P.R. China .
authors.forename: Zuohui
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: a Department of Neurology , Affiliated Hospital of Xuzhou Medical College , Xuzhou, Jiangsu Province , P.R. China .
authors.forename: Xinchun
authors.initials: X
authors.lastname: Ye
authors.affiliation: a Department of Neurology , Affiliated Hospital of Xuzhou Medical College , Xuzhou, Jiangsu Province , P.R. China .
authors.forename: Fang
authors.initials: F
authors.lastname: Hua
authors.affiliation: a Department of Neurology , Affiliated Hospital of Xuzhou Medical College , Xuzhou, Jiangsu Province , P.R. China .
authors.forename: Guiyun
authors.initials: G
authors.lastname: Cui
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001253:Astrocytes / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology*; D002463:Cell Membrane Permeability / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004790:Enzyme Induction / Q000187:drug effects; D051547:Heme Oxygenase-1 / Q000096:biosynthesis* / Q000378:metabolism; D006427:Hemin / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D006427:Hemin; D051547:Heme Oxygenase-1
keywords: Heme oxygenase-1; ZnPP-IX; apoptosis; hemin