title: Generation-dependent effect of PAMAM dendrimers on human insulin fibrillation and thermal stability.
abstract: We have studied the effect of polyamidoamine (PAMAM) dendrimers of various generations on the thermal stability and fibrillation of human insulin. Thermostability of human insulin used differential scanning calorimetry (DSC), which showed two phase-transitions for insulin at 60 and 82°C. After adding dendrimers at 0.6 μmol/l, the first peaks disappeared and the second peaks were higher. We posited that, in the presence of dendrimers, the dimers in the solution were transformed into hexamers. The effect of dendrimers on insulin fibrillation was monitored by measuring ThT fluorescence, and visualization of insulin fibrils by transmission electron microscopy (TEM) and atomic force microscopy (AFM). The effect of PAMAM dendrimers on insulin fibrillation was strongly dependent on the dendrimers generation and dendrimer:protein ratio.
authors.affiliation: Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, 141/143 Pomorska St., 90-236 Lodz, Poland.
authors.forename: Olga
authors.initials: O
authors.lastname: Nowacka
authors.affiliation: Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, 141/143 Pomorska St., 90-236 Lodz, Poland. Electronic address: milowska@biol.uni.lodz.pl.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Milowska
authors.affiliation: Department of Physical Chemistry, University of Lodz, 165 Pomorska St., 90-236, Poland.
authors.forename: Sylwia
authors.initials: S
authors.lastname: Belica-Pacha
authors.affiliation: Department of Physical Chemistry, University of Lodz, 165 Pomorska St., 90-236, Poland.
authors.forename: Bartlomiej
authors.initials: B
authors.lastname: Palecz
authors.affiliation: Department of Biophysics, Institute of Experimental Physics, Slovak Academy of Sciences, 47 Watsonova St., 040 01 Kosice, Slovakia.
authors.forename: Katarina
authors.initials: K
authors.lastname: Šipošová
authors.affiliation: Department of Biophysics, Institute of Experimental Physics, Slovak Academy of Sciences, 47 Watsonova St., 040 01 Kosice, Slovakia.
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Gazova
authors.affiliation: Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, 141/143 Pomorska St., 90-236 Lodz, Poland.
authors.forename: Maria
authors.initials: M
authors.lastname: Bryszewska
mesh_terms: D000682:Amyloid / Q000737:chemistry* / Q000648:ultrastructure; D002152:Calorimetry, Differential Scanning; D050091:Dendrimers / Q000737:chemistry*; D006801:Humans; D007328:Insulin / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D011108:Polymers / Q000737:chemistry; D066329:Protein Aggregates; D011485:Protein Binding; D055550:Protein Stability
chemical_list: D000682:Amyloid; D050091:Dendrimers; D007328:Insulin; C104700:PAMAM Starburst; D011108:Polymers; D066329:Protein Aggregates
keywords: DSC; Insulin; PAMAM dendrimers