title: Polycaprolactone foam functionalized with chitosan microparticles - a suitable scaffold for cartilage regeneration.
abstract: For biodegradable porous scaffolds to have a potential application in cartilage regeneration, they should enable cell growth and differentiation and should have adequate mechanical properties. In this study, our aim was to prepare biocompatible scaffolds with improved biomechanical properties. To this end, we have developed foam scaffolds from poly-epsilon-caprolactone (PCL) with incorporated chitosan microparticles. The scaffolds were prepared by a salt leaching technique from either 10 or 15 wt% PCL solutions containing 0, 10 and 20 wt% chitosan microparticles, where the same amount and size of NaCl was used as a porogen in all the cases. PCL scaffolds without and with low amounts of chitosan (0 and 10 wt% chitosan) showed higher DNA content than scaffolds with high amounts of chitosan during a 22-day experiment. 10 wt% PCL with 10 and 20 wt% chitosan showed significantly increased viscoelastic properties compared to 15 wt% PCL scaffolds with 0 and 10 wt% chitosan. Thus, 10 wt% PCL scaffolds with 0 wt% and 10 wt% chitosan are potential scaffolds for cartilage regeneration.
authors.affiliation: Faculty of Biomedical Engineering, Czech Technical University in Prague, Kladno, Czech Republic, Institute of Experimental Medicine of the Czech Academy of Sciences, Prague, Czech Republic. evafil@biomed.cas.cz.
authors.forename: E
authors.initials: E
authors.lastname: Filová
authors.forename: B
authors.initials: B
authors.lastname: Jakubcová
authors.forename: I
authors.initials: I
authors.lastname: Danilová
authors.forename: E
authors.initials: E
authors.lastname: KuŽelová Košťáková
authors.forename: T
authors.initials: T
authors.lastname: Jarošíková
authors.forename: O
authors.initials: O
authors.lastname: Chernyavskiy
authors.forename: J
authors.initials: J
authors.lastname: Hejda
authors.forename: M
authors.initials: M
authors.lastname: Handl
authors.forename: J
authors.initials: J
authors.lastname: Beznoska
authors.forename: A
authors.initials: A
authors.lastname: Nečas
authors.forename: J
authors.initials: J
authors.lastname: Rosina
authors.forename: E
authors.initials: E
authors.lastname: Amler
mesh_terms: D001672:Biocompatible Materials / Q000008:administration & dosage* / Q000737:chemistry; D002356:Cartilage / Q000166:cytology / Q000502:physiology*; D049109:Cell Proliferation / Q000187:drug effects / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D048091:Guided Tissue Regeneration / Q000379:methods*; D006801:Humans; D008863:Microspheres; D011091:Polyesters / Q000008:administration & dosage* / Q000737:chemistry; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials; D011091:Polyesters; C016240:polycaprolactone