title: Comparative Characterization of Vibrio cholerae O1 from Five Sub-Saharan African Countries Using Various Phenotypic and Genotypic Techniques.
abstract: We used standardized methodologies to characterize Vibrio cholerae O1 isolates from Guinea, Democratic Republic of the Congo (DRC), Togo, Côte d'Ivoire and Mozambique. We investigated 257 human isolates collected in 2010 to 2013. DRC isolates serotyped O1 Inaba, while isolates from other countries serotyped O1 Ogawa. All isolates were biotype El Tor and positive for cholera toxin. All isolates showed multidrug resistance but lacked ciprofloxacin resistance. Antimicrobial susceptibility profiles of isolates varied between countries. In particular, the susceptibility profile of isolates from Mozambique (East-Africa) included resistance to ceftriaxone and was distinctly different to the susceptibility profiles of isolates from countries located in West- and Central-Africa. Molecular subtyping of isolates using pulsed-field gel electrophoresis (PFGE) analysis showed a complex relationship among isolates. Some PFGE patterns were unique to particular countries and clustered by country; while other PFGE patterns were shared by isolates from multiple countries, indicating that the same genetic lineage is present in multiple countries. Our data add to a better understanding of cholera epidemiology in Africa.
authors.affiliation: Centre for Enteric Diseases, National Institute for Communicable Diseases, Division in the National Health Laboratory Service (NHLS), Johannesburg, South Africa.|Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa.
authors.forename: Anthony M
authors.initials: AM
authors.lastname: Smith
authors.affiliation: Agence de Médecine Préventive (AMP), Paris, France.
authors.forename: Berthe-Marie
authors.initials: BM
authors.lastname: Njanpop-Lafourcade
authors.affiliation: Agence de Médecine Préventive (AMP), Paris, France.
authors.forename: Martin A
authors.initials: MA
authors.lastname: Mengel
authors.affiliation: Agence de Médecine Préventive (AMP), Paris, France.
authors.forename: Bradford D
authors.initials: BD
authors.lastname: Gessner
authors.affiliation: Agence de Médecine Préventive (AMP), Paris, France.|Institut National d'Hygiène, Lomé, Togo.
authors.forename: Delphine
authors.initials: D
authors.lastname: Sauvageot
authors.affiliation: Institut National d'Hygiène, Lomé, Togo.
authors.forename: Bawimodom
authors.initials: B
authors.lastname: Bidjada
authors.affiliation: Institut National de Recherche Biomédicale, Kinshasa, Democratic Republic of Congo.
authors.forename: Berthe N
authors.initials: BN
authors.lastname: Miwanda
authors.affiliation: Institut National de Santé Publique, Conakry, Guinea.
authors.forename: Diallo M
authors.initials: DM
authors.lastname: Saliou
authors.affiliation: Institut Pasteur de Côte d'Ivoire, Abidjan, Côte d'Ivoire.
authors.forename: Adèle Kacou
authors.initials: AK
authors.lastname: N'Douba
authors.affiliation: National Institute of Health, Maputo, Mozambique.
authors.forename: José P
authors.initials: JP
authors.lastname: Langa
authors.affiliation: Centre for Enteric Diseases, National Institute for Communicable Diseases, Division in the National Health Laboratory Service (NHLS), Johannesburg, South Africa.|Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa.
authors.forename: Husna
authors.initials: H
authors.lastname: Ismail
authors.affiliation: Centre for Enteric Diseases, National Institute for Communicable Diseases, Division in the National Health Laboratory Service (NHLS), Johannesburg, South Africa.|Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa.
authors.forename: Nomsa
authors.initials: N
authors.lastname: Tau
authors.affiliation: Centre for Enteric Diseases, National Institute for Communicable Diseases, Division in the National Health Laboratory Service (NHLS), Johannesburg, South Africa.
authors.forename: Arvinda
authors.initials: A
authors.lastname: Sooka
authors.affiliation: Centre for Enteric Diseases, National Institute for Communicable Diseases, Division in the National Health Laboratory Service (NHLS), Johannesburg, South Africa.|Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa.
authors.forename: Karen H
authors.initials: KH
authors.lastname: Keddy
mesh_terms: D000293:Adolescent; D000328:Adult; D017954:Africa South of the Sahara / Q000453:epidemiology; D002771:Cholera / Q000453:epidemiology* / Q000382:microbiology*; D005260:Female; D005838:Genotype; D005843:Geography; D006801:Humans; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D010802:Phylogeny; D011159:Population Surveillance; D041241:Vibrio cholerae O1 / Q000145:classification* / Q000235:genetics*; D055815:Young Adult