title: Oxygen-glucose deprivation regulates BACE1 expression through induction of autophagy in Neuro-2a/APP695 cells.
abstract: Our previous findings have demonstrated that autophagy regulation can alleviate the decline of learning and memory by eliminating deposition of extracellular beta-amyloid peptide (Aβ) in the brain after stroke, but the exact mechanism is unclear. It is presumed that the regulation of beta-site APP-cleaving enzyme 1 (BACE1), the rate-limiting enzyme in metabolism of Aβ, would be a key site. Neuro-2a/amyloid precursor protein 695 (APP695) cell models of cerebral ischemia were established by oxygen-glucose deprivation to investigate the effects of Rapamycin (an autophagy inducer) or 3-methyladenine (an autophagy inhibitor) on the expression of BACE1. Either oxygen-glucose deprivation or Rapamycin down-regulated the expression of BACE1 while 3-methyladenine up-regulated BACE1 expression. These results confirm that oxygen-glucose deprivation down-regulates BACE1 expression in Neuro-2a/APP695 cells through the introduction of autophagy.
authors.affiliation: Department of Neurology, Sixth People's Hospital Affiliated to Shanghai Jiao Tong University, Shanghai, China.
authors.forename: Rong-Fu
authors.initials: RF
authors.lastname: Chen
authors.affiliation: Department of Neurology, Sixth People's Hospital Affiliated to Shanghai Jiao Tong University, Shanghai, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Department of Neurology, Sixth People's Hospital Affiliated to Shanghai Jiao Tong University, Shanghai, China.
authors.forename: Yin-Yi
authors.initials: YY
authors.lastname: Sun
authors.affiliation: Department of Neurology, Sixth People's Hospital Affiliated to Shanghai Jiao Tong University, Shanghai, China.
authors.forename: Ya-Meng
authors.initials: YM
authors.lastname: Sun
authors.affiliation: Department of Neurology, Sixth People's Hospital Affiliated to Shanghai Jiao Tong University, Shanghai, China.
authors.forename: Wen-Qi
authors.initials: WQ
authors.lastname: Chen
authors.affiliation: Zhoupu Hospital, Pudong New District, Shanghai, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Shi
authors.affiliation: Department of Neurology, Sixth People's Hospital Affiliated to Shanghai Jiao Tong University, Shanghai, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Shen
authors.affiliation: Zhoupu Hospital, Pudong New District, Shanghai, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Neurology, Sixth People's Hospital Affiliated to Shanghai Jiao Tong University, Shanghai, China ; Zhoupu Hospital, Pudong New District, Shanghai, China.
authors.forename: Kang-Yong
authors.initials: KY
authors.lastname: Liu
authors.affiliation: Department of Neurology, Sixth People's Hospital Affiliated to Shanghai Jiao Tong University, Shanghai, China.
authors.forename: Xiao-Jiang
authors.identifier: 0000-0003-2061-7262
authors.initials: XJ
authors.lastname: Sun
keywords: 3-methyladenine (3-MA); Rapamycin; autophagy; beta-amyloid peptide; beta-site APP-cleaving enzyme 1 (BACE1); brain Injury; cerebral ischemia; nerve regeneration; neural regeneration; oxygen-glucose deprivation; stroke