title: Biosynthesis of CdS nanoparticles: A fluorescent sensor for sulfate-reducing bacteria detection.
abstract: CdS nanoparticles were synthesized with an environmentally friendly method by taking advantage of the characteristic metabolic process of sulfate-reducing bacteria (SRB), and used as fluorescence labels for SRB detection. The presence of CdS nanoparticles was observed within and immediately surrounded bacterial cells, indicating CdS nanoparticles were synthesized both intracellularly and extracellularly. Moreover, fluorescent properties of microbial synthesized CdS nanoparticles were evaluated for SRB detection, and a linear relationship between fluorescence intensity and the logarithm of bacterial concentration was obtained in the range of from 1.0×10(2) to 1.0×10(7)cfu mL(-1). The proposed SRB detection method avoided the use of biological bio-recognition elements which are easy to lose their specific recognizing abilities, and the bacterial detection time was greatly shortened compared with the widely used MPN method which would take up to 15 days to accomplish the detection process.
authors.affiliation: Key Laboratory of Marine Environmental Corrosion and Bio-fouling, Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao 266071, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Qi
authors.affiliation: Key Laboratory of Marine Environmental Corrosion and Bio-fouling, Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao 266071, China. Electronic address: zhangdun@qdio.ac.cn.
authors.forename: Dun
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Marine Environmental Corrosion and Bio-fouling, Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao 266071, China; University of the Chinese Academy of Sciences, 19 (Jia) Yuquan Road, Beijing 100039, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zeng
authors.affiliation: Key Laboratory of Marine Environmental Corrosion and Bio-fouling, Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao 266071, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wan
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D019187:Cadmium Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D003901:Desulfovibrio / Q000737:chemistry / Q000302:isolation & purification*; D005456:Fluorescent Dyes / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry*; D013440:Sulfides / Q000138:chemical synthesis / Q000737:chemistry*
chemical_list: D019187:Cadmium Compounds; D005456:Fluorescent Dyes; D013440:Sulfides; C034939:cadmium sulfide
keywords: Biosynthesis; CdS nanoparticles; Detection; Sulfate-reducing bacteria