title: A Graphene and Aptamer Based Liquid Gated FET-Like Electrochemical Biosensor to Detect Adenosine Triphosphate.
abstract: Here we report successful demonstration of a FET-like electrochemical nano-biosensor to accurately detect ultralow concentrations of adenosine triphosphate. As a 2D material, graphene is a promising candidate due to its large surface area, biocompatibility, and demonstrated surface binding chemistries and has been employed as the conducting channel. A short 20-base DNA aptamer is used as the sensing element to ensure that the interaction between the analyte and the aptamer occurs within the Debye length of the electrolyte (PBS). Significant increase in the drain current with progressive addition of ATP is observed whereas for control experiments, no distinct change in the drain current occurs. The sensor is found to be highly sensitive in the nanomolar (nM) to micromolar ( μM) range with a high sensitivity of 2.55 μA (mM) (-1), a detection limit as low as 10 pM, and it has potential application in medical and biological settings to detect low traces of ATP. This simplistic design strategy can be further extended to efficiently detect a broad range of other target analytes.
authors.forename: Souvik
authors.initials: S
authors.lastname: Mukherjee
authors.forename: Xenia
authors.initials: X
authors.lastname: Meshik
authors.forename: Min
authors.initials: M
authors.lastname: Choi
authors.forename: Sidra
authors.initials: S
authors.lastname: Farid
authors.forename: Debopam
authors.initials: D
authors.lastname: Datta
authors.forename: Yi
authors.initials: Y
authors.lastname: Lan
authors.forename: Shripriya
authors.initials: S
authors.lastname: Poduri
authors.forename: Ketaki
authors.initials: K
authors.lastname: Sarkar
authors.forename: Undarmaa
authors.initials: U
authors.lastname: Baterdene
authors.forename: Ching-En
authors.initials: CE
authors.lastname: Huang
authors.forename: Yung Yu
authors.initials: YY
authors.lastname: Wang
authors.forename: Peter
authors.initials: P
authors.lastname: Burke
authors.forename: Mitra
authors.initials: M
authors.lastname: Dutta
authors.forename: Michael A
authors.initials: MA
authors.lastname: Stroscio
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis* / Q000737:chemistry; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation*; D004867:Equipment Design; D006108:Graphite / Q000737:chemistry*; D036103:Nanotechnology / Q000295:instrumentation*; D014173:Transistors, Electronic
chemical_list: D052157:Aptamers, Nucleotide; D006108:Graphite; D000255:Adenosine Triphosphate