title: Mannose-displaying fluorescent framboidal nanoparticles containing phenylboronic acid groups as a potential drug carrier for macrophage targeting.
abstract: Functional polymeric nanoparticles have been used for various applications in the biomaterials field. Recently, we reported phenylboronic acid-containing nanoparticles (PBA NPs) having an unique framboidal morphology, prepared in a single-step by the aqueous dispersion polymerization of N-acryloyl-3-aminophenylboronic acid (PBAAM) in the presence of poly(ethylene glycol) acrylamide (PEGAM) as a polymerizable dispersant and N,N'-methylenebisacrylamide (MBAM) as a crosslinker. In this study, we prepared mannosylated and fluorescent PBA NPs that could be used for different applications such as drug delivery and bioimaging. Fluorescent PBA NPs were synthesized by including the fluorescent Nile Blue acrylamide monomer in the reaction mixture during the dispersion polymerization of PBAAM. By using a carboxyl group-bearing PEGAM dispersant, carboxyl group-bearing PBA NPs were prepared that were modified with mannosamine to yield mannosylated PBA NPs. Cellular uptake studies showed that the mannosylated PBA NPs were selectively taken up by murine RAW264.7 macrophages. These results show that PBA NPs allow for flexible modification with various functionalities and could therefore be a potential platform for targeted delivery of drugs to macrophages.
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan; Frontier Research Base for Global Young Researchers, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan. Electronic address: urara.hasegawa@chem.eng.osaka-u.ac.jp.
authors.forename: Urara
authors.initials: U
authors.lastname: Hasegawa
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Inubushi
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Uyama
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan; Frontier Research Base for Global Young Researchers, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Taro
authors.initials: T
authors.lastname: Uematsu
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Susumu
authors.initials: S
authors.lastname: Kuwabata
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan; Frontier Research Center, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: André J
authors.initials: AJ
authors.lastname: van der Vlies
mesh_terms: D000818:Animals; D001897:Boronic Acids / Q000008:administration & dosage / Q000737:chemistry*; D002460:Cell Line; D004337:Drug Carriers; D005456:Fluorescent Dyes / Q000737:chemistry*; D008264:Macrophages / Q000187:drug effects*; D008358:Mannose / Q000737:chemistry*; D051379:Mice; D053758:Nanoparticles
chemical_list: D001897:Boronic Acids; D004337:Drug Carriers; D005456:Fluorescent Dyes; C010686:benzeneboronic acid; D008358:Mannose
keywords: Aqueous dispersion polymerization; Drug delivery; Fluorescence; Framboidal nanoparticle; Mannosylation