title: Leptin Genes in Blunt Snout Bream: Cloning, Phylogeny and Expression Correlated to Gonads Development.
abstract: To investigate the leptin related genes expression patterns and their possible function during the gonadal development in fish, the cDNA and genomic sequences of leptin, leptin receptor (leptinR), and leptin receptor overlapping transcript like-1 (leprotl1) were cloned and their expression levels were quantified in the different gonadal development stages of Megalobrama amblycephala. The results showed that the full length cDNA sequences of leptin, leptinR and leprotl1 were 953, 3432 and 1676 bp, coding 168, 1082, and 131 amino acid polypeptides, and the genomic sequences were 1836, 28,528 and 5480 bp, which respectively had 3, 15 and 4 exons, respectively. The phylogenetic analysis revealed that three genes were relatively conserved in fish species. Quantitative real-time PCR results showed that the three genes were ubiquitously expressed in all examined tissues during the different gonadal development stages. The leptin and leptinR took part in the onset of puberty, especially in female M. amblycephala, by increasing the expression levels in brain during the stage I to III of ovary. The expression levels of leptin and leptinR had significant differences between male and female in hypothalamic-pituitary-gonadal (HPG) axis tissues (p < 0.05). The leptinR had the same variation tendency with leptin, but the opposite changes of expression levels were found in leprotl1, which may resist the expression of leptinR for inhibiting the function of leptin in target organ. These findings revealed details about the possible role of these genes in regulating gonadal maturation in fish species.
authors.affiliation: College of Fisheries, Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education/Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, China. zhao253091640@hotmail.com.|Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan 430070, China. zhao253091640@hotmail.com.
authors.forename: Honghao
authors.initials: H
authors.lastname: Zhao
authors.affiliation: College of Fisheries, Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education/Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, China. Congzeng@live.cn.
authors.forename: Cong
authors.initials: C
authors.lastname: Zeng
authors.affiliation: College of Fisheries, Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education/Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, China. yishaokui@foxmail.com.|Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan 430070, China. yishaokui@foxmail.com.
authors.forename: Shaokui
authors.initials: S
authors.lastname: Yi
authors.affiliation: College of Fisheries, Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education/Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, China. wanshiming@webmail.hzau.edu.cn.|Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan 430070, China. wanshiming@webmail.hzau.edu.cn.
authors.forename: Shiming
authors.initials: S
authors.lastname: Wan
authors.affiliation: College of Fisheries, Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education/Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, China. ChenBX@haid.com.cn.|Hubei Bai Rong Improved Aquatic Seed Co., Ltd., Huanggang 438800, China. ChenBX@haid.com.cn.
authors.forename: Boxiang
authors.initials: B
authors.lastname: Chen
authors.affiliation: College of Fisheries, Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education/Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, China. gaozexia@hotmail.com.|Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan 430070, China. gaozexia@hotmail.com.
authors.forename: Zexia
authors.initials: Z
authors.lastname: Gao
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D019143:Evolution, Molecular; D005260:Female; D005399:Fishes / Q000145:classification / Q000235:genetics*; D015870:Gene Expression; D018507:Gene Expression Regulation, Developmental; D006066:Gonads / Q000378:metabolism; D020738:Leptin / Q000737:chemistry / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D038081:Organogenesis / Q000235:genetics; D010802:Phylogeny
chemical_list: D018076:DNA, Complementary; D020738:Leptin
keywords: Megalobrama amblycephala; cloning; gene expression; gonad development; leptin genes