title: Involvement of AMPK/SIRT1 pathway in anti-allodynic effect of troxerutin in CCI-induced neuropathic pain.
abstract: Neuropathic pain was regarded as a main form of chronic pain condition that remains difficult to treat. Conventional pharmacotherapy for neuropathic pain responsed vary and side effects limited their compliance. These prompted us to find new alternatives. In this study, we investigated the effect of troxerutin on treatment of CCI-induced neuropathic pain. Results showed that troxerutin significantly reversed mechanical allodynia and thermal hyperalgesia. In L4-6 spinal cord, troxerutin reduced the expression of INF-γ, IL-1β, TNF-α, and activation of NF-κB(p65). Immunofluorescence results showed that troxerutin significantly inhibited microglia activation induced by CCI surgery. Further, troxerutin treatment significantly induced AMPK activation and inhibited CCI-induced SIRT1 decrease. However, AMPK inhibitor compound C and SIRT1 inhibitor EX527 inhibited analgesic effect of troxerutin in CCI mice. This demonstrated the involvement of AMPK/SIRT1 pathway in anti-allodynic effect of troxerutin in CCI mice. Troxerutin could be developed as a potential therapeutic agent for neuropathic pain.
authors.affiliation: Department of Anesthesiology, Hunan Provincial Maternal and Child Health Hospital, Changsha 410000, Hunan Province, China. Electronic address: gyl0307@163.com.
authors.forename: Yulong
authors.initials: Y
authors.lastname: Gui
authors.affiliation: Department of Anesthesiology, Hunan Provincial Maternal and Child Health Hospital, Changsha 410000, Hunan Province, China.
authors.forename: Aiyuan
authors.initials: A
authors.lastname: Li
authors.affiliation: Department of Anesthesiology, Hunan Provincial Maternal and Child Health Hospital, Changsha 410000, Hunan Province, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Chen
authors.affiliation: Department of Radiology, the First Affiliated Hospital of Nanhua University, Hengyang 421001, Hunan Province, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Department of Ultrasound, the First Affiliated Hospital of Nanhua University, Hengyang 421001, Hunan Province, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Tang
authors.affiliation: Department of Anesthesiology, Hunan Provincial Maternal and Child Health Hospital, Changsha 410000, Hunan Province, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Anesthesiology, Hunan Provincial Maternal and Child Health Hospital, Changsha 410000, Hunan Province, China.
authors.forename: Shuxian
authors.initials: S
authors.lastname: Chen
authors.affiliation: Department of Endocrinology, Yongzhou Center Hospital, Yongzhou 425000, Hunan Province, China.
authors.forename: Shunshan
authors.initials: S
authors.lastname: Duan
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000378:metabolism*; D000818:Animals; D003250:Constriction; D005786:Gene Expression Regulation / Q000187:drug effects; D006896:Hydroxyethylrutoside / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use; D006930:Hyperalgesia / Q000150:complications / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D007371:Interferon-gamma / Q000378:metabolism; D053583:Interleukin-1beta / Q000378:metabolism; D008297:Male; D051379:Mice; D017628:Microglia / Q000187:drug effects / Q000473:pathology; D009437:Neuralgia / Q000150:complications* / Q000209:etiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000293:injuries; D015398:Signal Transduction / Q000187:drug effects*; D056565:Sirtuin 2 / Q000378:metabolism*; D051996:Transcription Factor RelA / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D006896:Hydroxyethylrutoside; D053583:Interleukin-1beta; D051996:Transcription Factor RelA; D014409:Tumor Necrosis Factor-alpha; C005865:troxerutin; D007371:Interferon-gamma; D055372:AMP-Activated Protein Kinases; C418743:Sirt2 protein, mouse; D056565:Sirtuin 2
keywords: AMPK; CCI(chronic constriction injury); Neuropathic pain; SIRT1; Troxerutin