title: Hierarchical micro/nanostructured titanium with balanced actions to bacterial and mammalian cells for dental implants.
abstract: A versatile strategy to endow dental implants with long-term antibacterial ability without compromising the cytocompatibility is highly desirable to combat implant-related infection. Silver nanoparticles (Ag NPs) have been utilized as a highly effective and broad-spectrum antibacterial agent for surface modification of biomedical devices. However, the high mobility and subsequent hazardous effects of the particles on mammalian cells may limit its practical applications. Thus, Ag NPs were immobilized on the surface of sand-blasted, large grit, and acid-etched (SLA) titanium by manipulating the atomic-scale heating effect of silver plasma immersion ion implantation. The silver plasma immersion ion implantation-treated SLA surface gave rise to both good antibacterial activity and excellent compatibility with mammalian cells. The antibacterial activity rendered by the immobilized Ag NPs was assessed using Fusobacterium nucleatum and Staphylococcus aureus, commonly suspected pathogens for peri-implant disease. The immobilized Ag NPs offered a good defense against multiple cycles of bacteria attack in both F. nucleatum and S. aureus, and the mechanism was independent of silver release. F. nucleatum showed a higher susceptibility to Ag NPs than S. aureus, which might be explained by the presence of different wall structures. Moreover, the immobilized Ag NPs had no apparent toxic influence on the viability, proliferation, and differentiation of rat bone marrow mesenchymal stem cells. These results demonstrated that good bactericidal activity could be obtained with very small quantities of immobilized Ag NPs, which were not detrimental to the mammalian cells involved in the osseointegration process, and promising for titanium-based dental implants with commercial SLA surfaces.
authors.affiliation: Department of Oral and Maxillofacial Implantology, Shanghai Key Laboratory of Stomatology, Shanghai Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, People's Republic of China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai University of Engineering Science, Shanghai, People's Republic of China.
authors.forename: Huiliang
authors.initials: H
authors.lastname: Cao
authors.affiliation: Department of Oral and Maxillofacial Implantology, Shanghai Key Laboratory of Stomatology, Shanghai Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, People's Republic of China.
authors.forename: Shichong
authors.initials: S
authors.lastname: Qiao
authors.affiliation: State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai University of Engineering Science, Shanghai, People's Republic of China ; School of Materials Engineering, Shanghai University of Engineering Science, Shanghai, People's Republic of China.
authors.forename: Manle
authors.initials: M
authors.lastname: Wang
authors.affiliation: Department of Oral and Maxillofacial Implantology, Shanghai Key Laboratory of Stomatology, Shanghai Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, People's Republic of China.
authors.forename: Yingxin
authors.initials: Y
authors.lastname: Gu
authors.affiliation: Department of Oral and Maxillofacial Implantology, Shanghai Key Laboratory of Stomatology, Shanghai Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, People's Republic of China.
authors.forename: Huiwen
authors.initials: H
authors.lastname: Luo
authors.affiliation: State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai University of Engineering Science, Shanghai, People's Republic of China.
authors.forename: Fanhao
authors.initials: F
authors.lastname: Meng
authors.affiliation: State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai University of Engineering Science, Shanghai, People's Republic of China.
authors.forename: Xuanyong
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Oral and Maxillofacial Implantology, Shanghai Key Laboratory of Stomatology, Shanghai Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, People's Republic of China.
authors.forename: Hongchang
authors.initials: H
authors.lastname: Lai
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects / Q000648:ultrastructure; D018441:Biofilms / Q000187:drug effects; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects; D002478:Cells, Cultured; D015921:Dental Implants; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D008856:Microscopy, Fluorescence; D053758:Nanoparticles / Q000737:chemistry*; D056951:Photoelectron Spectroscopy; D051381:Rats; D012834:Silver / Q000737:chemistry; D013211:Staphylococcus aureus / Q000187:drug effects; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D015921:Dental Implants; D012834:Silver; D014025:Titanium; D000469:Alkaline Phosphatase
keywords: antibacterial effects; cytotoxicity; plasma immersion ion implantation; silver nanoparticles; surface modification