title: Newly isolated alkalophilic Advenella species bioaugmented in activated sludge for high p-cresol removal.
abstract: In this work, an alkalophilic bacterium (LVX-4) capable of using p-cresol as sole source of carbon and energy was screened and isolated from soil polluted by used oil. Phylogenetic (16S rRNA) and phenotypic characterization using Biolog GN microplates and API 20NE strips indicated that LVX-4 strain is a new Advenella species. It showed both the capability to degrade of p-cresol at high concentrations (750 mg/L) and to use p-cresol for growth in a pH from 7 to 10, although the optimum pH was 9. Moreover bioaugmentation of activated sludge with this strain lead to the complete removal of p-cresol in less than 100 h. This is the first study that shows the potential of Advenella sp. to be bioaugmented in activated sludge system for p-cresol biodegradation.
authors.affiliation: Cyprus University of Technology, Department of Environmental Science and Technology, 95 Eirinis Str., P.O. BOX 50329, 3603 Lemesos, Cyprus.
authors.forename: Eleni
authors.initials: E
authors.lastname: Xenofontos
authors.affiliation: University of Bucharest, Department of Genetics, Intrarea Postocalelor nr. 1-3, OP 060101 Bucharest, Romania; University of Agronomic Science and Veterinary Medicine, Faculty of Biotechnologies, Bulevardul Marasti nr. 59, Bucharest, cod: 011464, Romania.
authors.forename: Ana-Maria
authors.initials: AM
authors.lastname: Tanase
authors.affiliation: University of Bucharest, Department of Genetics, Intrarea Postocalelor nr. 1-3, OP 060101 Bucharest, Romania.
authors.forename: Ileana
authors.initials: I
authors.lastname: Stoica
authors.affiliation: Cyprus University of Technology, Department of Environmental Science and Technology, 95 Eirinis Str., P.O. BOX 50329, 3603 Lemesos, Cyprus. Electronic address: Ioannis.vyrides@cut.ac.cy.
authors.forename: Ioannis
authors.initials: I
authors.lastname: Vyrides
mesh_terms: D042401:Alcaligenaceae / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism*; D001673:Biodegradation, Environmental; D003408:Cresols / Q000378:metabolism*; D059392:Petroleum Pollution; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012722:Sewage / Q000382:microbiology*; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*
chemical_list: D003408:Cresols; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D012989:Soil Pollutants; C032538:4-cresol