title: Mitochondrial D-loop Variations in Infertile Women Undergoing in Vitro Fertilization-Embryo Transfer.
abstract: OBJECTIVE
  To show a high frequency of human mitochondrial DNA (mtDNA) D-loop variations present in infertile women undergoing in vitro fertilization-embryo transfer (IVF-ET) and the variation's potential relevance as a predictive marker in the IVF-ET.
  
  
  METHODS
  The entire mtDNA D-loop region was amplified in 3 overlapping polymerase chain reaction fragments, and the variations were evaluated through direct DNA sequencing methods in 156 infertile women undergoing IVF-ET.
  
  
  RESULTS
  A total of 48 variations were found at 47 positions in the D-loop of all patients. The incidence of variations (particularly 16191 C --> T [p = 0.0321, 16519 T --> C [p = 0.0431, 326 A --> G [p = 0.0031, and 432 A --> C [p = 0.042) was significantly higher in patients with top quality embryo rate 41.84% (p = .0.004). However, variations were not significantly associated with the fertilization rate (p = 0.846), cleavage rate (p = 1.000), pregnancy rate (p = 0.839), implantation rate (p = 0.911), or miscarriage rate (p = 0.658).
  
  
  CONCLUSIONS
  Our data suggest that the increase in mtDNA variations in the peripheral blood from infertile women could have a predictive value for the outcomes of infertile women undergoing IVF-ET.
authors.forename: Yue
authors.initials: Y
authors.lastname: Lin
authors.forename: Yizuo
authors.initials: Y
authors.lastname: Chen
authors.forename: Jing
authors.initials: J
authors.lastname: Du
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Huang
authors.forename: Junzhao
authors.initials: J
authors.lastname: Zhao
authors.forename: Xiaoxi
authors.initials: X
authors.lastname: Sun
mesh_terms: D000022:Abortion, Spontaneous / Q000235:genetics; D000328:Adult; D004272:DNA, Mitochondrial / Q000235:genetics*; D010064:Embryo Implantation; D004624:Embryo Transfer / Q000379:methods; D005260:Female; D005306:Fertilization / Q000235:genetics; D005307:Fertilization in Vitro / Q000379:methods; D014644:Genetic Variation; D006801:Humans; D007247:Infertility, Female / Q000235:genetics* / Q000628:therapy; D011247:Pregnancy; D018873:Pregnancy Rate
chemical_list: D004272:DNA, Mitochondrial