title: Effects of ferrous carbamoyl glycine on iron state and absorption in an iron-deficient rat model.
abstract: An iron-deficient rat model was established and used to determine the effects of different iron sources on iron metabolism and absorption. Iron-deficient rats were assigned to one of three treatment groups, and their diet was supplemented with deionized water (control), Fe-CGly, or FeSO4 for 8 days via intragastric administration. Blood samples were obtained for analysis of iron-related properties, and the small intestine and liver were removed for quantitative reverse transcription PCR of genes related to iron metabolism. The serum total iron-binding capacity (TIBC) levels of rats in Fe-CGly and FeSO4 supplementation groups was lower (P < 0.05) than that of the rats in the control group. The rats in Fe-CGly group exhibited higher (P < 0.05) plasma Fe and ferritin levels and lower (P < 0.05) TIBC levels compared with the rats in FeSO4 groups. The relative expression of liver hepcidin increased (P < 0.05) by tenfold and 80-fold in the Fe-CGly and FeSO4 groups, respectively, whereas divalent metal transporter 1, duodenal cytochrome b, and ferroportin 1 expression decreased (P < 0.05) in the duodenum in both Fe-CGly and FeSO4 group. A comparison between Fe-CGly and FeSO4 group showed that iron regulatory protein 1 (IRP1) and iron regulatory protein (IRP2) expressions were reduced (P < 0.05) in rats administered FeSO4 than in rats administered with Fe-Cgly. These results indicate that Fe-CGly rapidly improves the blood iron status and that IRP1 and IRP2 may play an important role in the intestinal absorption of Fe-CGly.
authors.affiliation: Hunan Provincial Engineering Research Center of Healthy Livestock, Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, Hunan 410125 China.
authors.forename: Yuzhe
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Hunan Provincial Engineering Research Center of Healthy Livestock, Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, Hunan 410125 China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Sun
authors.affiliation: Hunan Provincial Engineering Research Center of Healthy Livestock, Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, Hunan 410125 China.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Xie
authors.affiliation: Hunan Provincial Engineering Research Center of Healthy Livestock, Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, Hunan 410125 China.|State Key Laboratory of Food Science and Technology and College of Life Science and Food Engineering, Nanchang University, Nanchang, 330031 China.|Guangzhou Tanke BIO-TECH Co. Ltd., Guangzhou, 510800 China.
authors.forename: Xugang
authors.initials: X
authors.lastname: Shu
authors.affiliation: Hunan Provincial Engineering Research Center of Healthy Livestock, Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, Hunan 410125 China.|Department of Animal Nutrition, College of Animal Science and Livestock Production, PMB 2240 Abeokuta, Nigeria.
authors.forename: Abimbola Oladele
authors.initials: AO
authors.lastname: Oso
authors.affiliation: State Key Laboratory of Food Science and Technology and College of Life Science and Food Engineering, Nanchang University, Nanchang, 330031 China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Ruan
authors.affiliation: State Key Laboratory of Food Science and Technology and College of Life Science and Food Engineering, Nanchang University, Nanchang, 330031 China.
authors.forename: Ze-Yuan
authors.initials: ZY
authors.lastname: Deng
authors.affiliation: Hunan Provincial Engineering Research Center of Healthy Livestock, Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, Hunan 410125 China.|State Key Laboratory of Food Science and Technology and College of Life Science and Food Engineering, Nanchang University, Nanchang, 330031 China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wu
authors.affiliation: Hunan Provincial Engineering Research Center of Healthy Livestock, Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, Hunan 410125 China.|State Key Laboratory of Food Science and Technology and College of Life Science and Food Engineering, Nanchang University, Nanchang, 330031 China.
authors.forename: Yulong
authors.initials: Y
authors.lastname: Yin
keywords: FeSO4; Ferrous carbamoyl glycine; Hepcidin; IRP1/IRP2; SD rats