title: Sequence-specific 1H, 13C and 15N backbone resonance assignments of the plakin repeat domain of human envoplakin.
abstract: The plakin repeat domain is a distinctive hallmark of the plakin superfamily of proteins, which are found within all epithelial tissues. Plakin repeat domains mediate the interactions of these proteins with the cell cytoskeleton and are critical for the maintenance of tissue integrity. Despite their biological importance, no solution state resonance assignments are available for any homologue. Here we report the essentially complete (1)H, (13)C and (15)N backbone chemical shift assignments of the singular 22 kDa plakin repeat domain of human envoplakin, providing the means to investigate its interactions with ligands including intermediate filaments.
authors.affiliation: Henry Wellcome Building for Biomolecular NMR Spectroscopy, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK.
authors.forename: Mark
authors.initials: M
authors.lastname: Jeeves
authors.affiliation: Henry Wellcome Building for Biomolecular NMR Spectroscopy, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK.
authors.forename: Claudia
authors.initials: C
authors.lastname: Fogl
authors.affiliation: MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge, CB2 0QH, UK.|School of Cancer Sciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK.
authors.forename: Caezar
authors.initials: C
authors.lastname: Al-Jassar
authors.affiliation: School of Cancer Sciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK.
authors.forename: Martyn
authors.initials: M
authors.lastname: Chidgey
authors.affiliation: Henry Wellcome Building for Biomolecular NMR Spectroscopy, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK. overduin@ualberta.ca.|Department of Biochemistry, Faculty of Medicine and Dentistry, University of Alberta, 474 Medical Sciences Building, Edmonton, AB, T6G 2H7, Canada. overduin@ualberta.ca.
authors.forename: Michael
authors.initials: M
authors.lastname: Overduin
mesh_terms: D000595:Amino Acid Sequence; D006801:Humans; D008565:Membrane Proteins / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular; D051179:Plakins / Q000737:chemistry*; D011498:Protein Precursors / Q000737:chemistry*; D020449:Repetitive Sequences, Amino Acid
chemical_list: D008565:Membrane Proteins; D051179:Plakins; D011498:Protein Precursors; C100754:envoplakin
keywords: Backbone resonance assignment; Cornified envelope; Cytoskeleton; Envoplakin; Plakin; Plakin repeat domain