title: Microfluidic Bioprinting of Heterogeneous 3D Tissue Constructs Using Low-Viscosity Bioink.
abstract: A novel bioink and a dispensing technique for 3D tissue-engineering applications are presented. The technique incorporates a coaxial extrusion needle using a low-viscosity cell-laden bioink to produce highly defined 3D biostructures. The extrusion system is then coupled to a microfluidic device to control the bioink arrangement deposition, demonstrating the versatility of the bioprinting technique. This low-viscosity cell-responsive bioink promotes cell migration and alignment within each fiber organizing the encapsulated cells.
authors.affiliation: Biomaterials Innovation Research Center, Harvard Medical School, Brigham and Women's Hospital, Cambridge, MA, 02139, USA.|Harvard-MIT Division of Health Sciences and Technology, Cambridge, MA, 02139, USA.|Department of Chemistry, Sapienza Università di Roma, Piazzale Aldo Moro 5, Rome, 00185, Italy.
authors.forename: Cristina
authors.initials: C
authors.lastname: Colosi
authors.affiliation: Biomaterials Innovation Research Center, Harvard Medical School, Brigham and Women's Hospital, Cambridge, MA, 02139, USA.|Harvard-MIT Division of Health Sciences and Technology, Cambridge, MA, 02139, USA.|Wyss Institute for Biologically Inspired Engineering, Boston, MA, 02115, USA.
authors.forename: Su Ryon
authors.initials: SR
authors.lastname: Shin
authors.affiliation: Biomaterials Innovation Research Center, Harvard Medical School, Brigham and Women's Hospital, Cambridge, MA, 02139, USA.|Harvard-MIT Division of Health Sciences and Technology, Cambridge, MA, 02139, USA.
authors.forename: Vijayan
authors.initials: V
authors.lastname: Manoharan
authors.affiliation: Biomaterials Innovation Research Center, Harvard Medical School, Brigham and Women's Hospital, Cambridge, MA, 02139, USA.|Harvard-MIT Division of Health Sciences and Technology, Cambridge, MA, 02139, USA.|Programa de Doctorado en Biomedicina, Universidad de los Andes, Santiago, 7620001, Chile.
authors.forename: Solange
authors.initials: S
authors.lastname: Massa
authors.affiliation: Department of Chemistry, Sapienza Università di Roma, Piazzale Aldo Moro 5, Rome, 00185, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Costantini
authors.affiliation: Department of Chemistry, Sapienza Università di Roma, Piazzale Aldo Moro 5, Rome, 00185, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Barbetta
authors.affiliation: Biomaterials Innovation Research Center, Harvard Medical School, Brigham and Women's Hospital, Cambridge, MA, 02139, USA.|Harvard-MIT Division of Health Sciences and Technology, Cambridge, MA, 02139, USA.|Wyss Institute for Biologically Inspired Engineering, Boston, MA, 02115, USA.
authors.forename: Mehmet Remzi
authors.initials: MR
authors.lastname: Dokmeci
authors.affiliation: Department of Chemistry, Sapienza Università di Roma, Piazzale Aldo Moro 5, Rome, 00185, Italy.
authors.forename: Mariella
authors.initials: M
authors.lastname: Dentini
authors.affiliation: Biomaterials Innovation Research Center, Harvard Medical School, Brigham and Women's Hospital, Cambridge, MA, 02139, USA.|Harvard-MIT Division of Health Sciences and Technology, Cambridge, MA, 02139, USA.|Wyss Institute for Biologically Inspired Engineering, Boston, MA, 02115, USA.|Department of Bioindustrial Technologies, College of Animal Bioscience and Technology, Konkuk University, Hwayang-dong, Kwangjin-gu, Seoul, 143-701, Republic of Korea.|Department of Physics, King Abdulaziz University, Jeddah, 21569, Saudi Arabia.
authors.forename: Ali
authors.initials: A
authors.lastname: Khademhosseini
mesh_terms: D000464:Alginates / Q000737:chemistry; D062028:Bioprinting / Q000295:instrumentation / Q000379:methods*; D002470:Cell Survival / Q000528:radiation effects; D005109:Extracellular Matrix / Q000737:chemistry / Q000378:metabolism; D005780:Gelatin / Q000737:chemistry; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D018613:Microscopy, Confocal; D066330:Printing, Three-Dimensional; D023822:Tissue Engineering; D054457:Tissue Scaffolds; D014466:Ultraviolet Rays; D014783:Viscosity
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D020723:Glucuronic Acid; D005780:Gelatin
keywords: 3D tissue engineering; bioinks; bioprinting; microfluidics; vascular tissue engineering