title: Bioactive borate glass promotes the repair of radius segmental bone defects by enhancing the osteogenic differentiation of BMSCs.
abstract: Bioactive borate glass (BG) has emerged as a promising alternative for bone regeneration due to its high osteoinductivity, osteoconductivity, compressive strength, and biocompatibility. However, the role of BG in large segmental bone repair is unclear and little is known about the underlying mechanism of BG's osteoinductivity. In this study, we demonstrated that BG possessed pro-osteogenic effects in an experimental model of critical-sized radius defects. Transplanting BG to radius defects resulted in better repair of bone defects as compared to widely used β-TCP. Histological and morphological analysis indicated that BG significantly enhanced new bone formation. Furthermore, the degradation rate of the BG was faster than that of β-TCP, which matched the higher bone regeneration rate. In addition, ions from BG enhanced cell viability, ALP activity, and osteogenic-related genes expression. Mechanistically, the critical genes Smad1/5 and Dlx5 in the BMP pathway and p-Smad1/5 proteins were significantly elevated after BG transplantation, and these effects could be blocked by the BMP/Smad specific inhibitor. Taken together, our findings suggest that BG could repair large segmental bone defects through activating the BMP/Smad pathway and osteogenic differentiation in BMSCs.
authors.affiliation: Institute of Microsurgery on Extremities, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai 200233, People's Republic of China. Department of Orthopedics, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai 200233, People's Republic of China. These authors contributed equally.
authors.forename: Jieyuan
authors.initials: J
authors.lastname: Zhang
authors.forename: Junjie
authors.initials: J
authors.lastname: Guan
authors.forename: Changqing
authors.initials: C
authors.lastname: Zhang
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.forename: Wenhai
authors.initials: W
authors.lastname: Huang
authors.forename: Shangchun
authors.initials: S
authors.lastname: Guo
authors.forename: Xin
authors.initials: X
authors.lastname: Niu
authors.forename: Zongping
authors.initials: Z
authors.lastname: Xie
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
mesh_terms: D020341:Absorbable Implants; D000328:Adult; D000368:Aged; D000818:Animals; D018786:Bone Substitutes / Q000138:chemical synthesis*; D002454:Cell Differentiation; D019245:Compressive Strength; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005260:Female; D005898:Glass / Q000737:chemistry*; D006801:Humans; D008297:Male; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology; D008875:Middle Aged; D010012:Osteogenesis / Q000502:physiology*; D011817:Rabbits; D011885:Radius Fractures / Q000473:pathology / Q000628:therapy*; D013314:Stress, Mechanical; D054457:Tissue Scaffolds; D016896:Treatment Outcome
chemical_list: D018786:Bone Substitutes