title: Plasticizer DBP Activates NLRP3 Inflammasome through the P2X7 Receptor in HepG2 and L02 Cells.
abstract: Ditubyl phthalate (DBP), one of the most widely used plasticizers, can migrate out to contaminate our bodies and environment. A number of studies have showed that DBP is closely related to liver pathological changes and diseases. Inflammasomes are multiprotein complexes composed of procaspase and pattern recognition receptors such as Nucleotide oligomerization domain (NOD) like receptor family, pyrin domain containing 3 (NLRP3). Activation of NLRP3 inflammasome is implicated in the pathogeneses of liver damage. The aim of this study was to determine the effects of DBP on NLRP3 inflammasome. We found that DBP triggered the activation of NLRP3 inflammasome in hepatocyte cell lines. By using Ca-074-Me, N-acetylcysteine and KN-62, we observed that the P2X7 receptor participated in the DBP-induced activation of NLRP3 inflammasome. DBP could also trigger the ATP release. In conclusion, we demonstrated that DBP is one of the activator of NLRP3 inflammasome and may play an important role in liver damage.
authors.affiliation: Cardiovascular Research Center, Xi'an Jiaotong University, Xi'an, 710061, People's Republic of China.
authors.forename: Jiahua
authors.initials: J
authors.lastname: Ni
authors.affiliation: Cardiovascular Research Center, Xi'an Jiaotong University, Xi'an, 710061, People's Republic of China.
authors.forename: Zihui
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Cardiovascular Research Center, Xi'an Jiaotong University, Xi'an, 710061, People's Republic of China.
authors.forename: Xiaoqin
authors.initials: X
authors.lastname: Luo
authors.affiliation: Cardiovascular Research Center, Xi'an Jiaotong University, Xi'an, 710061, People's Republic of China. xiaolei.0122@163.com.
authors.forename: Lei
authors.initials: L
authors.lastname: Xiao
authors.affiliation: The Advanced Institute for Medical Sciences, Dalian Medical University, Dalian, 116044, People's Republic of China. nanpingwang2003@yahoo.com.
authors.forename: Nanping
authors.initials: N
authors.lastname: Wang
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D003993:Dibutyl Phthalate / Q000633:toxicity*; D056945:Hep G2 Cells; D006801:Humans; D058847:Inflammasomes / Q000378:metabolism*; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein / Q000378:metabolism*; D010968:Plasticizers / Q000633:toxicity*; D058486:Receptors, Purinergic P2X7 / Q000378:metabolism*
chemical_list: D058847:Inflammasomes; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; C453881:NLRP3 protein, human; D010968:Plasticizers; D058486:Receptors, Purinergic P2X7; D003993:Dibutyl Phthalate; D000255:Adenosine Triphosphate
keywords: DBP; NLRP3 Inflammasome; P2X7 Receptor; Plasticizer