title: Quantitative Analysis of Tissue Samples by Combining iTRAQ Isobaric Labeling with Selected/Multiple Reaction Monitoring (SRM/MRM).
abstract: Mass spectrometry-based phosphoproteomics is an indispensible technique used in the discovery and quantification of phosphorylation events on proteins in biological samples. The application of this technique to tissue samples is especially useful for the discovery of biomarkers as well as biological studies. We herein describe the application of a large-scale phosphoproteome analysis and SRM/MRM-based quantitation to develop a strategy for the systematic discovery and validation of biomarkers using tissue samples.
authors.affiliation: Laboratory for Synthetic Biology, RIKEN Quantitative Biology Center, Osaka, Japan.|Laboratory of Proteome Research, National Institutes of Biomedical Innovation, Health and Nutrition, Osaka, Japan.
authors.forename: Ryohei
authors.initials: R
authors.lastname: Narumi
authors.affiliation: Laboratory of Proteome Research, National Institutes of Biomedical Innovation, Health and Nutrition, Osaka, Japan. tomonaga@nibiohn.go.jp.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Tomonaga
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D006801:Humans; D007553:Isotope Labeling; D013058:Mass Spectrometry; D010449:Peptide Mapping; D010750:Phosphoproteins / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D040901:Proteomics / Q000379:methods*; D013379:Substrate Specificity; D057188:Workflow
chemical_list: D015415:Biomarkers; D010750:Phosphoproteins; D011494:Protein Kinases
keywords: IMAC; MRM; Phosphoproteome; SRM; iTRAQ