title: ITS-2 sequences-based identification of Trichogramma species in South America.
abstract: ITS2 (Internal transcribed spacer 2) sequences have been used in systematic studies and proved to be useful in providing a reliable identification of Trichogramma species. DNAr sequences ranged in size from 379 to 632 bp. In eleven T. pretiosum lines Wolbachia-induced parthenogenesis was found for the first time. These thelytokous lines were collected in Peru (9), Colombia (1) and USA (1). A dichotomous key for species identification was built based on the size of the ITS2 PCR product and restriction analysis using three endonucleases (EcoRI, MseI and MaeI). This molecular technique was successfully used to distinguish among seventeen native/introduced Trichogramma species collected in South America.
authors.affiliation: Centro Nacional de Pesquisa de Algodão, Empresa Brasileira de Pesquisa Agropecuária, Campina Grande, PB, Brazil.
authors.forename: R P
authors.initials: RP
authors.lastname: Almeida
authors.affiliation: Department of Entomology, University of California, Riverside, CA, USA.
authors.forename: R
authors.initials: R
authors.lastname: Stouthamer
mesh_terms: D000818:Animals; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D008969:Molecular Sequence Data; D010312:Parthenogenesis; D017422:Sequence Analysis, DNA; D013020:South America; D014863:Wasps / Q000145:classification* / Q000235:genetics / Q000382:microbiology / Q000502:physiology*; D020577:Wolbachia / Q000502:physiology
chemical_list: D021903:DNA, Ribosomal Spacer