title: Antibacterial efficacy of a cetylpyridinium chloride-based mouthrinse against Fusobacterium nucleatum and in vitro plaques.
abstract: OBJECTIVE
  To assess the antimicrobial effects of a fluoride-free and alcohol-free mouthrinse containing 0.075% CPC (test rinse, TR) compared with an otherwise-identical CPC-free control rinse (CR).
  
  
  METHODS
  Activity against laboratory cultures of Fusobacterium nucleatum, a bacterium associated with gingival disease, was determined using viable counting following 30-second exposures to TR and CR. Effects against intact saliva-derived plaque biofilms were quantified using confocal microscopy coupled with three-dimensional image analyses (viability profiling).
  
  
  RESULTS
  Short exposures to TR caused significant inactivation of F. nucleatum, as determined by viable counting (c. 3 log reduction compared to the control rinse, P < 0.05). Confocal microscopy revealed extensive inactivation of complex oral biofilms following treatment with TR; biofilms were significantly less viable than those exposed to CR and three-dimensional images revealed extensive zones of dead bacteria even within plaque depths. In conclusion, this investigation demonstrates that the CPC-containing mouthrinse has significant antibacterial efficacy against oral bacteria associated with gingival disease and significantly inactivated plaque biofilm in comparison to a relevant control.
authors.forename: Joe
authors.initials: J
authors.lastname: Latimer
authors.forename: Jodie L
authors.initials: JL
authors.lastname: Munday
authors.forename: Kara M
authors.initials: KM
authors.lastname: Buzza
authors.forename: Prem K
authors.initials: PK
authors.lastname: Sreenivasan
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: McBain
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002594:Cetylpyridinium / Q000494:pharmacology*; D015169:Colony Count, Microbial; D003773:Dental Plaque / Q000517:prevention & control*; D016967:Fusobacterium nucleatum / Q000187:drug effects*; D006801:Humans; D066298:In Vitro Techniques; D008826:Microbial Sensitivity Tests; D009067:Mouthwashes
chemical_list: D000900:Anti-Bacterial Agents; D009067:Mouthwashes; D002594:Cetylpyridinium