title: Detection of single amino acid mutation in human breast cancer by disordered plasmonic self-similar chain.
abstract: Control of the architecture and electromagnetic behavior of nanostructures offers the possibility of designing and fabricating sensors that, owing to their intrinsic behavior, provide solutions to new problems in various fields. We show detection of peptides in multicomponent mixtures derived from human samples for early diagnosis of breast cancer. The architecture of sensors is based on a matrix array where pixels constitute a plasmonic device showing a strong electric field enhancement localized in an area of a few square nanometers. The method allows detection of single point mutations in peptides composing the BRCA1 protein. The sensitivity demonstrated falls in the picomolar (10(-12) M) range. The success of this approach is a result of accurate design and fabrication control. The residual roughness introduced by fabrication was taken into account in optical modeling and was a further contributing factor in plasmon localization, increasing the sensitivity and selectivity of the sensors. This methodology developed for breast cancer detection can be considered a general strategy that is applicable to various pathologies and other chemical analytical cases where complex mixtures have to be resolved in their constitutive components.
authors.affiliation: Bio-Nanotechnology and Engineering for Medicine (BIONEM), Department of Experimental and Clinical Medicine, University of Magna Graecia Viale Europa, Germaneto, Catanzaro 88100, Italy.
authors.forename: Maria Laura
authors.initials: ML
authors.lastname: Coluccio
authors.affiliation: Bio-Nanotechnology and Engineering for Medicine (BIONEM), Department of Experimental and Clinical Medicine, University of Magna Graecia Viale Europa, Germaneto, Catanzaro 88100, Italy. ; Department of Electrical Engineering and Information Technology, University of Naples, Naples 80125, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Gentile
authors.affiliation: Physical Sciences and Engineering (PSE) and Biological and Environment Science and Engineering Divisions (BESE), King Abdullah University of Science and Technology, Thuwal 23955-6900, Kingdom of Saudi Arabia.
authors.forename: Gobind
authors.initials: G
authors.lastname: Das
authors.affiliation: Bio-Nanotechnology and Engineering for Medicine (BIONEM), Department of Experimental and Clinical Medicine, University of Magna Graecia Viale Europa, Germaneto, Catanzaro 88100, Italy.
authors.forename: Annalisa
authors.initials: A
authors.lastname: Nicastri
authors.affiliation: Bio-Nanotechnology and Engineering for Medicine (BIONEM), Department of Experimental and Clinical Medicine, University of Magna Graecia Viale Europa, Germaneto, Catanzaro 88100, Italy.
authors.forename: Angela Mena
authors.initials: AM
authors.lastname: Perri
authors.affiliation: Bio-Nanotechnology and Engineering for Medicine (BIONEM), Department of Experimental and Clinical Medicine, University of Magna Graecia Viale Europa, Germaneto, Catanzaro 88100, Italy.
authors.forename: Patrizio
authors.initials: P
authors.lastname: Candeloro
authors.affiliation: Bio-Nanotechnology and Engineering for Medicine (BIONEM), Department of Experimental and Clinical Medicine, University of Magna Graecia Viale Europa, Germaneto, Catanzaro 88100, Italy.
authors.forename: Gerardo
authors.initials: G
authors.lastname: Perozziello
authors.affiliation: Nanostructures, Istituto Italiano di Tecnologia, via Morego 30, Genova 16163, Italy.
authors.forename: Remo
authors.initials: R
authors.lastname: Proietti Zaccaria
authors.affiliation: PRIMALIGHT, Electrical Engineering Division, King Abdullah University of Science and Technology, Thuwal 23955-6900, Kingdom of Saudi Arabia.
authors.forename: Juan Sebastian Totero
authors.initials: JS
authors.lastname: Gongora
authors.affiliation: Physical Sciences and Engineering (PSE) and Biological and Environment Science and Engineering Divisions (BESE), King Abdullah University of Science and Technology, Thuwal 23955-6900, Kingdom of Saudi Arabia.
authors.forename: Salma
authors.initials: S
authors.lastname: Alrasheed
authors.affiliation: PRIMALIGHT, Electrical Engineering Division, King Abdullah University of Science and Technology, Thuwal 23955-6900, Kingdom of Saudi Arabia.
authors.forename: Andrea
authors.initials: A
authors.lastname: Fratalocchi
authors.affiliation: Physical Sciences and Engineering (PSE) and Biological and Environment Science and Engineering Divisions (BESE), King Abdullah University of Science and Technology, Thuwal 23955-6900, Kingdom of Saudi Arabia.
authors.forename: Tania
authors.initials: T
authors.lastname: Limongi
authors.affiliation: Bio-Nanotechnology and Engineering for Medicine (BIONEM), Department of Experimental and Clinical Medicine, University of Magna Graecia Viale Europa, Germaneto, Catanzaro 88100, Italy.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Cuda
authors.affiliation: Bio-Nanotechnology and Engineering for Medicine (BIONEM), Department of Experimental and Clinical Medicine, University of Magna Graecia Viale Europa, Germaneto, Catanzaro 88100, Italy. ; Physical Sciences and Engineering (PSE) and Biological and Environment Science and Engineering Divisions (BESE), King Abdullah University of Science and Technology, Thuwal 23955-6900, Kingdom of Saudi Arabia.
authors.forename: Enzo
authors.initials: E
authors.lastname: Di Fabrizio
keywords: BRCA1 protein; nano lens; plasmonics; self-similar chain device; single molecule detection; single point mutation detection