title: Development and Application of a Gel-Based Immunoassay for the Rapid Screening of Salbutamol and Ractopamine Residues in Pork.
abstract: Salbutamol (SAL) and ractopamine (RAC) have been illegally used to promote protein synthesis and to increase the feed conversion rate in livestock. However, the residues of SAL and RAC could cause potential hazards for human health. The Ministry of Agriculture of China banned the use of SAL and RAC as growth promoters. In this paper, we provide detailed information on developing a rapid and sensitive gel-based immunoassay for on-site screening of SAL and RAC residues in pork. The detection time was shortened to 20 min. The limits of detection were 0.5 μg/kg for both SAL and RAC by visual detection, whereas the quantitative gel-based immunoassay enabled the detection of SAL (0.051 μg/kg) and RAC (0.020 μg/kg) in spiked pork samples. The gel-based immunoassay showed promise as a multiplexed immunoassay for on-site surveilling of SAL and RAC residues in pork.
authors.affiliation: Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, Beijing Laboratory for Food Quality and Safety , 100193 Beijing, People's Republic of China.
authors.forename: Chenglong
authors.initials: C
authors.lastname: Li
authors.affiliation: Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, Beijing Laboratory for Food Quality and Safety , 100193 Beijing, People's Republic of China.
authors.forename: Jingya
authors.initials: J
authors.lastname: Li
authors.affiliation: Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, Beijing Laboratory for Food Quality and Safety , 100193 Beijing, People's Republic of China.|The Engineering Laboratory of Synthetic Biology, Key Laboratory of Biomedical Engineering, School of Medicine, Health Science Center, Shenzhen University , 518060 Shenzhen, People's Republic of China.
authors.forename: Wenxiao
authors.initials: W
authors.lastname: Jiang
authors.affiliation: Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, Beijing Laboratory for Food Quality and Safety , 100193 Beijing, People's Republic of China.
authors.forename: Suxia
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, Beijing Laboratory for Food Quality and Safety , 100193 Beijing, People's Republic of China.|National Reference Laboratory for Veterinary Drug Residues , 100193 Beijing, People's Republic of China.
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Shen
authors.affiliation: Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, Beijing Laboratory for Food Quality and Safety , 100193 Beijing, People's Republic of China.|National Reference Laboratory for Veterinary Drug Residues , 100193 Beijing, People's Republic of China.
authors.forename: Kai
authors.initials: K
authors.lastname: Wen
authors.affiliation: Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, Beijing Laboratory for Food Quality and Safety , 100193 Beijing, People's Republic of China.
authors.forename: Zhanhui
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000420:Albuterol / Q000032:analysis*; D000818:Animals; D004350:Drug Residues / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis*; D006133:Growth Substances / Q000032:analysis*; D007118:Immunoassay / Q000379:methods*; D008460:Meat / Q000032:analysis*; D010627:Phenethylamines / Q000032:analysis*; D013552:Swine
chemical_list: D006133:Growth Substances; D010627:Phenethylamines; C060897:ractopamine; D000420:Albuterol
keywords: gel-based multiplexed immunoassay; pork; ractopamine; salbutamol