title: Quantitation of Parathyroid Hormone in Serum or Plasma by Liquid Chromatography-Tandem Mass Spectrometry.
abstract: Parathyroid hormone (PTH), an 84 amino acid peptide hormone, is an important regulator of calcium homeostasis. Quantitation of PTH in serum is useful for the diagnosis of primary hyperparathyroidism, hypoparathyroidism, and for monitoring osteodystrophy in patients with renal failure. The biological activity of PTH arises from binding of PTH (N terminus) to its target receptor (D'Amour et al., Kidney Int 68: 998-1007, 2005). Several C-terminal and N-terminal fragments circulate in normal subjects. Recent studies have demonstrated that accurate quantitation of PTH fragments may be of clinical value. In this chapter a mass spectrometry based method for quantitation of PTH(1-84) is described. This method involves immunoaffinity capture of PTH followed by trypsinization and quantitation of PTH-specific tryptic peptides by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The N-terminal tryptic peptide, PTH(1-13) as surrogate of 1-84 PTH, is used for quantitation.
authors.affiliation: Departement of Pathology, University of Michigan Health System, Ann Arbor, MN, 48109-5054, USA.
authors.forename: Hemamalini
authors.initials: H
authors.lastname: Ketha
authors.affiliation: Department of Laboratory Medicine and Pathology, Mayo Clinic, 200 2nd Street SW, Rochester, MN, 55905, USA. Singh.Ravinder@mayo.edu.
authors.forename: Ravinder J
authors.initials: RJ
authors.lastname: Singh
mesh_terms: D053000:Analytic Sample Preparation Methods; D055910:Antibodies, Immobilized / Q000737:chemistry / Q000276:immunology; D001774:Blood Chemical Analysis / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D008863:Microspheres; D010281:Parathyroid Hormone / Q000097:blood* / Q000276:immunology / Q000302:isolation & purification*; D011137:Polystyrenes / Q000737:chemistry; D013223:Statistics as Topic; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D055910:Antibodies, Immobilized; D010281:Parathyroid Hormone; D011137:Polystyrenes
keywords: Immunocapture LC-MS/MS; LC-MS/MS; Parathyroid hormone quantitation