title: Identification and characterization of a novel chitinase with antifungal activity from 'Baozhu' pear (Pyrus ussuriensis Maxim.).
abstract: A novel chitinase from the 'Baozhu' pear was found, purified, and characterized in this report. This chitinase was a monomer with a molecular mass of 28.9 kDa. Results of the internal peptide sequence analyses classify this chitinase as a class III chitinase. In the enzymatic hydrolytic assay, this chitinase could hydrolyze chitin derivatives into di-N-acetylchitobiose (GlcNAc2) as a major product in the initial phase, as well as hydrolyze GlcNAc2 into N-acetylglucosamine (GlcNAc), which represents both chitobiosidase and β-N-acetylglucosaminase activity. Biological analyses showed that this chitinase exhibits strong antifungal activity toward agricultural pathogenic fungi. In total, chitinase from 'Baozhu' pear is a novel bifunctional chitinase that could be a potential fungicide in the biological control of plant diseases.
authors.affiliation: Yunnan Institute of Food Safety, Kunming University of Science and Technology, Kunming, Yunnan 650500, China. Electronic address: hanpeng320@Kmust.edu.cn.
authors.forename: Peng
authors.initials: P
authors.lastname: Han
authors.affiliation: Yunnan Institute of Food Safety, Kunming University of Science and Technology, Kunming, Yunnan 650500, China.
authors.forename: Chengcheng
authors.initials: C
authors.lastname: Yang
authors.affiliation: Yunnan Institute of Food Safety, Kunming University of Science and Technology, Kunming, Yunnan 650500, China.
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Liang
authors.affiliation: Yunnan Institute of Food Safety, Kunming University of Science and Technology, Kunming, Yunnan 650500, China.
authors.forename: Lirong
authors.initials: L
authors.lastname: Li
mesh_terms: D000935:Antifungal Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D002686:Chitin / Q000737:chemistry / Q000378:metabolism; D002688:Chitinases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D004187:Disaccharides / Q000378:metabolism; D005658:Fungi / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D010935:Plant Diseases / Q000382:microbiology; D010940:Plant Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D031989:Pyrus / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics; D013696:Temperature
chemical_list: D000935:Antifungal Agents; D004187:Disaccharides; D010940:Plant Proteins; D002686:Chitin; C010913:N,N-diacetylchitobiose; D002688:Chitinases; C110892:chitinase, class III
keywords: Antifungal activity; Bifunctional enzyme; Chitinase; Purification; ʻBaozhu’ pear