title: Alteration of the Metabolome Profile in Endothelial Cells by Overexpression of miR-143/145.
abstract: Communication between endothelial cells (ECs) and smooth muscle cells (SMCs) via miR-143/145 clusters is vital to vascular stability. Previous research demonstrates that miR-143/145 released from ECs can regulate SMC proliferation and migration. In addition, a recent study has found that SMCs also have the capability of manipulating EC function via miR-143/145. In the present study, we artificially increased the expression of miR-143/145 in ECs, to mimic a similar change caused by miR-143/145 released by SMCs, and applied untargeted metabolomics analysis, aimed at investigating the consequential effect of miR-143/145 overexpression. Our results showed that miR-143/145 overexpression alters the levels of metabolites involved in energy production, DNA methylation, and oxidative stress. These changed metabolites indicate that metabolic pathways, such as the SAM cycle and TCA cycle, exhibit significant differences from the norm with miR-143/145 overexpression.
authors.affiliation: Department of Cardiac Surgery, Zhongshan Hospital of Fudan University and Shanghai Institute of Cardiovascular Diseases, Shanghai 200032, P.R. China.
authors.forename: Wenshuo
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Cardiac Surgery, Zhongshan Hospital of Fudan University and Shanghai Institute of Cardiovascular Diseases, Shanghai 200032, P.R. China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Cardiothoracic Surgery, Huashan Hospital of Fudan University, Shanghai 200040, P.R. China.
authors.forename: Yiqing
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Cardiothoracic Surgery, Huashan Hospital of Fudan University, Shanghai 200040, P.R. China.
authors.forename: Liewen
authors.initials: L
authors.lastname: Pang
authors.affiliation: Department of Cardiothoracic Surgery, Huashan Hospital of Fudan University, Shanghai 200040, P.R. China.
authors.forename: Jiechun
authors.initials: J
authors.lastname: Huang
authors.affiliation: Department of Radiology, Huashan Hospital of Fudan University, Shanghai 200040, P.R. China.
authors.forename: Hongyue
authors.initials: H
authors.lastname: Tao
authors.affiliation: Department of Cardiothoracic Surgery, Huashan Hospital of Fudan University, Shanghai 200040, P.R. China.
authors.forename: Xiaotian
authors.initials: X
authors.lastname: Sun
authors.affiliation: Department of Cardiac Surgery, Zhongshan Hospital of Fudan University and Shanghai Institute of Cardiovascular Diseases, Shanghai 200032, P.R. China.
authors.forename: Chen
authors.initials: C
authors.lastname: Liu
mesh_terms: D019175:DNA Methylation; D042783:Endothelial Cells / Q000378:metabolism*; D015870:Gene Expression; D006801:Humans; D055442:Metabolome; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D018384:Oxidative Stress
chemical_list: C542359:MIRN143 microRNA, human; C541010:MIRN145 microRNA, human; D035683:MicroRNAs
keywords: Cellular communication; MicroRNA; Smooth muscle cell; Untargeted metabolomics; Vascular biology