title: [Effects of tumor necrosis factor-α on release of MMP-3, MMP-9, and interleukin-17 in mouse bone marrow-derived mast cells in vitro].
abstract: OBJECTIVE
  To investigate the effect of tumor necrosis factor-α (TNF-α) on the release of matrix metalloproteinase-3 (MMP-3), MMP-9, and interleukin-17 (IL-17) in cultured mouse bone marrow-derived mast cells (BMMCs) in vitro.
  
  
  METHODS
  Primarily cultured mouse BMMCs at 8 weeks were exposed PBS (control) or TNF-α at the concentrations of 2, 10, or 50 ng/mL for 12 or 24 h. Real-time PCR was performed to detect the mRNA expressions of MMP-3, MMP-9, and IL-17 in the exposed cells.
  
  
  RESULTS
  A 12-hour exposure of the BMMCs to TNF-α caused significantly increased expressions of MMP-3, MMP-9, and IL-17 in a concentration-dependent manner (P<0.05). Prolonged exposures of the cells to 2 and 10 TNF-α for 24 h further increased MMP-3, MMP-9, and IL-17 mRNA expressions, but exposure to 50 ng/mL TNF-α for 24 h increased only MMP-3 and MMP-9 expressions but not IL-17 mRNA expression.
  
  
  CONCLUSIONS
  TNF-α treatment of primarily cultured BMMCs can significantly increase the cellular expressions of MMP-3, MMP-9, and IL-17 mRNA in a time- and dose-dependent manner.
authors.affiliation: Department of Rheumatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.E-mail: 578320375@qq.com.
authors.forename: Yujiao
authors.initials: Y
authors.lastname: Chen
authors.forename: Qingqing
authors.initials: Q
authors.lastname: Ouyang
authors.forename: Ran
authors.initials: R
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: Wu
authors.forename: Jinjun
authors.initials: J
authors.lastname: Zhao
authors.forename: Min
authors.initials: M
authors.lastname: Yang
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000378:metabolism; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D020381:Interleukin-17 / Q000378:metabolism*; D008407:Mast Cells / Q000378:metabolism*; D019278:Matrix Metalloproteinase 3 / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D051379:Mice; D012333:RNA, Messenger; D060888:Real-Time Polymerase Chain Reaction; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
chemical_list: D020381:Interleukin-17; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D019278:Matrix Metalloproteinase 3; C511769:Mmp3 protein, mouse; D020780:Matrix Metalloproteinase 9; C501830:Mmp9 protein, mouse