title: Absence of myeloperoxidase and CD8 positive cells in colorectal cancer infiltrates identifies patients with severe prognosis.
abstract: Colorectal cancer (CRC) infiltration by cells expressing myeloperoxidase (MPO) or CD8 positive T lymphocytes has been shown to be independently associated with favorable prognosis. We explored the relationship occurring between CD8+ and MPO+ cell CRC infiltration, its impact on clinical-pathological features and its prognostic significance in a tissue microarray (TMA) including 1,162 CRC. We observed that CRC showing high MPO+ cell infiltration are characterized by a prognosis as favorable as that of cancers with high CD8+ T cell infiltration. However, MPO+ and CD8+ CRC infiltrating cells did not synergize in determining a more favorable outcome, as compared with cancers showing MPOhigh/CD8lowor MPOlow/CD8highinfiltrates. Most importantly, we identified a subgroup of CRC with MPOlow/CD8lowtumor infiltration characterized by a particularly severe prognosis. Intriguingly, although MPO+ and CD8+ cells did not co-localize in CRC infiltrates, an increased expression of TIA-1 and granzyme-B was detectable in T cells infiltrating CRC with high MPO+ cell density.
authors.affiliation: Department of Surgery; University Hospital Basel ; Basel, Switzerland ; Institute of Surgical Research and Hospital Management, Department of Biomedicine; University of Basel , Basel, Switzerland.
authors.forename: Silvio
authors.initials: S
authors.lastname: Däster
authors.affiliation: Institute of Pathology; University Hospital Basel ; Basel, Switzerland.
authors.forename: Serenella
authors.initials: S
authors.lastname: Eppenberger-Castori
authors.affiliation: Department of Surgery; University Hospital Basel ; Basel, Switzerland ; Institute of Surgical Research and Hospital Management, Department of Biomedicine; University of Basel , Basel, Switzerland.
authors.forename: Christian
authors.initials: C
authors.lastname: Hirt
authors.affiliation: Department of Surgery; University Hospital Basel ; Basel, Switzerland ; Institute of Surgical Research and Hospital Management, Department of Biomedicine; University of Basel , Basel, Switzerland.
authors.forename: Savas D
authors.initials: SD
authors.lastname: Soysal
authors.affiliation: Department of Surgery; University Hospital Basel ; Basel, Switzerland.
authors.forename: Tarik
authors.initials: T
authors.lastname: Delko
authors.affiliation: Department of Surgery; University Hospital Basel ; Basel, Switzerland ; Institute of Surgical Research and Hospital Management, Department of Biomedicine; University of Basel , Basel, Switzerland.
authors.forename: Christian A
authors.initials: CA
authors.lastname: Nebiker
authors.affiliation: Department of Surgery; University Hospital Basel ; Basel, Switzerland.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Weixler
authors.affiliation: Institute of Surgical Research and Hospital Management, Department of Biomedicine; University of Basel , Basel, Switzerland.
authors.forename: Francesca
authors.initials: F
authors.lastname: Amicarella
authors.affiliation: Institute of Surgical Research and Hospital Management, Department of Biomedicine; University of Basel , Basel, Switzerland.
authors.forename: Giandomenica
authors.initials: G
authors.lastname: Iezzi
authors.affiliation: Institute of Surgical Research and Hospital Management, Department of Biomedicine; University of Basel , Basel, Switzerland.
authors.forename: Valeria
authors.initials: V
authors.lastname: Governa
authors.affiliation: Institute of Surgical Research and Hospital Management, Department of Biomedicine; University of Basel , Basel, Switzerland.
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Padovan
authors.affiliation: Institute of Surgical Research and Hospital Management, Department of Biomedicine; University of Basel , Basel, Switzerland.
authors.forename: Valentina
authors.initials: V
authors.lastname: Mele
authors.affiliation: Institute of Translational Pharmacology, Department of Biomedicine; National Research Council , Rome, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Sconocchia
authors.affiliation: Institute of Surgical Research and Hospital Management, Department of Biomedicine; University of Basel , Basel, Switzerland.
authors.forename: Michael
authors.initials: M
authors.lastname: Heberer
authors.affiliation: Institute of Pathology; University Hospital Basel ; Basel, Switzerland.
authors.forename: Luigi
authors.initials: L
authors.lastname: Terracciano
authors.affiliation: Department of Surgery; University Hospital Basel ; Basel, Switzerland.
authors.forename: Christoph
authors.initials: C
authors.lastname: Kettelhack
authors.affiliation: Department of Surgery; University Hospital Basel ; Basel, Switzerland.
authors.forename: Daniel
authors.initials: D
authors.lastname: Oertli
authors.affiliation: Institute of Surgical Research and Hospital Management, Department of Biomedicine; University of Basel , Basel, Switzerland.
authors.forename: Giulio C
authors.initials: GC
authors.lastname: Spagnoli
authors.affiliation: Department of Surgery; University Hospital Basel ; Basel, Switzerland ; Indiana University School of Medicine South Bend, Indiana University Health Goshen Center for Cancer Care , Goshen, IN, USA.
authors.forename: Urs
authors.initials: U
authors.lastname: von Holzen
authors.affiliation: Institute of Pathology; University Hospital Basel ; Basel, Switzerland.
authors.forename: Luigi
authors.initials: L
authors.lastname: Tornillo
authors.affiliation: Department of Surgery; University Hospital Basel ; Basel, Switzerland ; Institute of Surgical Research and Hospital Management, Department of Biomedicine; University of Basel , Basel, Switzerland.
authors.forename: Raoul A
authors.initials: RA
authors.lastname: Droeser
keywords: CD8+; human colorectal cancer; myeloperoxidase; prognostic markers; tissue microarray