title: In Situ Hybridization of Estrogen Receptors α and β and GPER in the Human Testis.
abstract: In situ hybridization (ISH) is an excellent method for detecting RNA in histological sections, both to detect gene expression and to assign gene expression to a distinct cell population. Therefore, ISH may be used in basic cell biology to detect the expression of certain genes within a tissue containing various cell populations. Here, we describe the detection and cellular localization of three estrogen receptors, both isoforms of the genomic estrogen receptor (ERα and ERβ) as well as the membrane-bound G-protein-coupled estrogen receptor 1 (GPER) in the human testis.
authors.affiliation: Institute for Veterinary Anatomy, Histology and Embryology, Justus Liebig University Giessen, Frankfurter Straße 98, 35392, Gießen, Germany. daniela.fietz@vetmed.uni-giessen.de.
authors.forename: Daniela
authors.initials: D
authors.lastname: Fietz
authors.affiliation: Institute for Veterinary Anatomy, Histology and Embryology, Justus Liebig University Giessen, Frankfurter Straße 98, 35392, Gießen, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Bergmann
authors.affiliation: Institute for Veterinary Anatomy, Histology and Embryology, Justus Liebig University Giessen, Frankfurter Straße 98, 35392, Gießen, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Hartmann
mesh_terms: D047628:Estrogen Receptor alpha / Q000235:genetics*; D047629:Estrogen Receptor beta / Q000235:genetics*; D005786:Gene Expression Regulation; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D016133:Polymerase Chain Reaction; D015347:RNA Probes; D011960:Receptors, Estrogen / Q000235:genetics*; D043562:Receptors, G-Protein-Coupled / Q000235:genetics*; D013737:Testis / Q000737:chemistry*; D057188:Workflow
chemical_list: C506487:ESR1 protein, human; C000600034:ESR2 protein, human; D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; C109584:GPER1 protein, human; D015347:RNA Probes; D011960:Receptors, Estrogen; D043562:Receptors, G-Protein-Coupled
keywords: Cellular localization; Estrogen receptor s; Gene expression; In situ hybridization; Molecular biology; Testis