title: Effect of Chicoric Acid on Mast Cell-Mediated Allergic Inflammation in Vitro and in Vivo.
abstract: Chicoric acid (dicaffeoyl-tartaric acid), is a natural phenolic compound found in a number of plants, such as chicory (Cichorium intybus) and Echinacea (Echinacea purpurea), which possesses antioxidant, anti-inflammatory, antiviral, and analgesic activities. Although these biological effects of chicoric acid have been investigated, there are no reports of its antiallergic-related anti-inflammatory effects in human mast cells (HMC)-1 or anaphylactic activity in a mouse model. Therefore, we investigated the antiallergic-related anti-inflammatory effect of chicoric acid and its underlying mechanisms of action using phorbol-12-myristate 13-acetate plus calcium ionophore A23187 (PMACI)-stimulated HMC-1 cells. Chicoric acid decreased the mRNA expression of pro-inflammatory cytokines, such as tumor necrosis factor (TNF)-α, interleukin (IL)-6, and IL-1β. We studied the inhibitory effects of chicoric acid on the nuclear translocation of nuclear factor kappa B (NF-κB) and activation of caspase-1. However, mitogen-activated protein kinase (MAPK) activation was not sufficient to abrogate the stimulus. In addition, we investigated the ability of chicoric acid to inhibit compound 48/80-induced systemic anaphylaxis in vivo. Oral administration of chicoric acid at 20 mg/kg inhibited histamine release and protected mice against compound 48/80-induced anaphylactic mortality. These results suggest that chicoric acid has an antiallergic-related anti-inflammatory effect that involves modulating mast cell-mediated allergic responses. Therefore, chicoric acid could be an efficacious agent for allergy-related inflammatory disorders.
authors.affiliation: Department of Pharmacology, College of Oriental Medicine, Sangji University , Wonju-si, Gangwon-do 220-702, Republic of Korea.|Department of Herbal Medicine Resources, Chonbuk National University , Iksan 570-752, Republic of Korea.
authors.forename: Na Young
authors.initials: NY
authors.lastname: Lee
authors.affiliation: Department of Pharmacology, College of Oriental Medicine, Sangji University , Wonju-si, Gangwon-do 220-702, Republic of Korea.
authors.forename: Kyung-Sook
authors.initials: KS
authors.lastname: Chung
authors.affiliation: Department of Herbal Medicine Resources, Chonbuk National University , Iksan 570-752, Republic of Korea.
authors.forename: Jong Sik
authors.initials: JS
authors.lastname: Jin
authors.forename: Keuk Soo
authors.initials: KS
authors.lastname: Bang
authors.affiliation: Department of Oriental Ophthalmology & Otolaryngology & Dermatology Medicine, College of Oriental Medicine, Sangji University , Wonju-si, Gangwon-do 220-702, Republic of Korea.
authors.forename: Ye-Jin
authors.initials: YJ
authors.lastname: Eom
authors.affiliation: Department of Oriental Ophthalmology & Otolaryngology & Dermatology Medicine, College of Oriental Medicine, Sangji University , Wonju-si, Gangwon-do 220-702, Republic of Korea.
authors.forename: Chul-Hee
authors.initials: CH
authors.lastname: Hong
authors.affiliation: Department of Agroindustrial Technology, Lambung Mangkurat University , Banjarbaru, Indonesia.
authors.forename: Agung
authors.initials: A
authors.lastname: Nugroho
authors.affiliation: Department of Pharmaceutical Engineering, College of Health Sciences, Sangji University , Wonju, Republic of Korea.
authors.forename: Hee-Jun
authors.initials: HJ
authors.lastname: Park
authors.affiliation: Department of Pharmacology, College of Oriental Medicine, Sangji University , Wonju-si, Gangwon-do 220-702, Republic of Korea.
authors.forename: Hyo-Jin
authors.initials: HJ
authors.lastname: An
mesh_terms: D000818:Animals; D018926:Anti-Allergic Agents / Q000494:pharmacology*; D002109:Caffeic Acids / Q000494:pharmacology*; D020170:Caspase 1 / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D006636:Histamine Release / Q000187:drug effects; D006801:Humans; D007249:Inflammation / Q000188:drug therapy*; D053583:Interleukin-1beta / Q000037:antagonists & inhibitors; D008297:Male; D008407:Mast Cells / Q000187:drug effects*; D051379:Mice; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D015394:Molecular Structure; D016328:NF-kappa B / Q000037:antagonists & inhibitors; D010703:Phorbol Esters / Q000494:pharmacology; D013386:Succinates / Q000494:pharmacology*; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: D018926:Anti-Allergic Agents; D002109:Caffeic Acids; D016207:Cytokines; D053583:Interleukin-1beta; D016328:NF-kappa B; D010703:Phorbol Esters; D013386:Succinates; C119222:phorbol-12-myristate; D003189:p-Methoxy-N-methylphenethylamine; D020928:Mitogen-Activated Protein Kinases; D048051:p38 Mitogen-Activated Protein Kinases; D020170:Caspase 1; C100435:chicoric acid