title: Polydimethylsiloxane microfluidic chemiluminescence immunodevice with the signal amplification strategy for sensitive detection of human immunoglobin G.
abstract: A polydimethylsiloxane (PDMS) microfluidic chemiluminescence (CL) immunodevice for sensitive detection of human immunoglobin G (IgG) with the signal amplification strategy was developed in this work. The immunodevice was prepared by covalently immobilizing capture antibodies (Abs) on the silanized microchannel of microfluidic chip. Gold nanoparticles (AuNPs) functionalized with a high molar ratio of horseradish peroxidase (HRP) were used as an Ab label for signal amplification. Using a sandwich immunoassay, the multi-HRP conjugated AuNPs can catalyze the luminol-H2O2 CL system to achieve the high sensitivity. In addition, the double spiral flow-channel was adopted here, which can still contribute to the high sensitivity. Based on signal amplification strategy, the performance of human IgG tests revealed a lower detection limit (DL) of 0.03ng/mL and showed an increase of 7.4-fold in detection sensitivity compared to a commercial Ab-HRP conjugation. This microfluidic immunodevice can provide an alternative approach for sensitive detection of human IgG in the field of clinic diagnostic and therapeutic.
authors.affiliation: Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an, 710062, China.
authors.forename: Huifang
authors.initials: H
authors.lastname: Li
authors.affiliation: Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an, 710062, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an, 710062, China. Electronic address: weiliu@126.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an, 710062, China.
authors.forename: Weiru
authors.initials: W
authors.lastname: Chu
authors.affiliation: Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an, 710062, China.
authors.forename: Yumei
authors.initials: Y
authors.lastname: Guo
mesh_terms: D055910:Antibodies, Immobilized / Q000032:analysis* / Q000737:chemistry; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D006046:Gold / Q000737:chemistry; D006735:Horseradish Peroxidase / Q000737:chemistry; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis* / Q000737:chemistry; D056656:Lab-On-A-Chip Devices; D049449:Luminescence; D053768:Metal Nanoparticles / Q000737:chemistry
chemical_list: D055910:Antibodies, Immobilized; D004129:Dimethylpolysiloxanes; D007074:Immunoglobulin G; C013830:baysilon; D006046:Gold; D006735:Horseradish Peroxidase
keywords: Chemiluminescence; Microfluidic immunodevice; Multi-HRP AuNPs amplification; PDMS; human IgG