title: Evaluating the effect of 20-hydroxyecdysone (20HE) on mechanistic target of rapamycin complex 1 (mTORC1) signaling in the skeletal muscle and liver of rats.
abstract: Phytoecdysteroids such as 20-hydroxyecdysone (20HE) are nutritional supplements marketed as enhancers of lean body mass. In this study the impact of 20HE ingestion on protein kinase B/Akt-mechanistic target of rapamycin complex 1 signaling in the skeletal muscle and liver of male rats was found to be limited. Bioavailability of 20HE, whether consumed alone or with leucine, also remained low at all doses ingested. Additional work is necessary to clarify 20HE mechanism of action in vivo.
authors.affiliation: a Department of Nutritional Sciences, Rutgers University, New Brunswick, NJ 08901, USA.
authors.forename: Tracy G
authors.initials: TG
authors.lastname: Anthony
authors.affiliation: a Department of Nutritional Sciences, Rutgers University, New Brunswick, NJ 08901, USA.
authors.forename: Emily T
authors.initials: ET
authors.lastname: Mirek
authors.affiliation: a Department of Nutritional Sciences, Rutgers University, New Brunswick, NJ 08901, USA.
authors.forename: Albert Raouf
authors.initials: AR
authors.lastname: Bargoud
authors.affiliation: a Department of Nutritional Sciences, Rutgers University, New Brunswick, NJ 08901, USA.
authors.forename: Lindsey
authors.initials: L
authors.lastname: Phillipson-Weiner
authors.affiliation: a Department of Nutritional Sciences, Rutgers University, New Brunswick, NJ 08901, USA.
authors.forename: Christopher M
authors.initials: CM
authors.lastname: DeOliveira
authors.affiliation: a Department of Nutritional Sciences, Rutgers University, New Brunswick, NJ 08901, USA.
authors.forename: Berish
authors.initials: B
authors.lastname: Wetstein
authors.affiliation: b Department of Plant Biology and Pathology, Rutgers University, New Brunswick, NJ 08901, USA.
authors.forename: Brittany L
authors.initials: BL
authors.lastname: Graf
authors.affiliation: b Department of Plant Biology and Pathology, Rutgers University, New Brunswick, NJ 08901, USA.
authors.forename: Peter E
authors.initials: PE
authors.lastname: Kuhn
authors.affiliation: b Department of Plant Biology and Pathology, Rutgers University, New Brunswick, NJ 08901, USA.
authors.forename: Ilya
authors.initials: I
authors.lastname: Raskin
mesh_terms: D000818:Animals; D001682:Biological Availability; D019587:Dietary Supplements; D004305:Dose-Response Relationship, Drug; D004441:Ecdysterone / Q000493:pharmacokinetics / Q000494:pharmacology*; D007930:Leucine / Q000494:pharmacology; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D000076222:Mechanistic Target of Rapamycin Complex 1; D046912:Multiprotein Complexes / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000201:enzymology; D010766:Phosphorylation; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000187:drug effects; D058570:TOR Serine-Threonine Kinases / Q000378:metabolism*
chemical_list: D046912:Multiprotein Complexes; D004441:Ecdysterone; D000076222:Mechanistic Target of Rapamycin Complex 1; D051057:Proto-Oncogene Proteins c-akt; D058570:TOR Serine-Threonine Kinases; D007930:Leucine
keywords: 4E-BP1; PKB/Akt; leucine; mTOR; phytoecdysteroids; phytoecdystéroïdes