title: A reversible fluorescence probe for detection of ClO(-)/AA redox cycle in aqueous solution and in living cells.
abstract: Based on the reversible redox reaction of 4-amino-2,2,6,6-tetramethyl-piperidin-1-ol with oxidative ClO(-) and reductive ascorbic acid (AA), a reversible fluorescent probe, incorporating 4-amino-2,2,6,6-tetramethyl-piperidin-1-ol group into a 7-nitrobenzoxadiazole (NBD) fluorophore, was synthesized and studied for the cyclic detection of ClO(-)/AA in aqueous solution. The NBD-based probe displayed sensitive and selective On-Off-On fluorescent responses to ClO(-)/AA and was successfully applied to monitor the ClO(-)/AA redox cycle in living HeLa cells under physiological conditions.
authors.affiliation: Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
authors.forename: Jiamin
authors.initials: J
authors.lastname: Wang
authors.affiliation: Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Ni
authors.affiliation: Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, PR China. Electronic address: sjshao@licp.cas.cn.
authors.forename: Shijun
authors.initials: S
authors.lastname: Shao
mesh_terms: D001205:Ascorbic Acid / Q000032:analysis* / Q000737:chemistry / Q000494:pharmacology; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D006997:Hypochlorous Acid / Q000032:analysis* / Q000737:chemistry / Q000494:pharmacology; D061848:Optical Imaging; D010069:Oxadiazoles / Q000737:chemistry*; D010084:Oxidation-Reduction; D010880:Piperidines / Q000737:chemistry*; D012996:Solutions
chemical_list: C000590808:2,2',6,6'-tetramethylpiperidin-1-ol; C526864:7-nitrobenz-2-oxa-1,3-diazole; D005456:Fluorescent Dyes; D010069:Oxadiazoles; D010880:Piperidines; D012996:Solutions; D006997:Hypochlorous Acid; D001205:Ascorbic Acid
keywords: 7-Nitrobenzoxadiazole (NBD); Bioimaging; ClO(−); Fluorescent probe; Redox cycle