title: Chromatin Immunoprecipitation with Estrogen Receptor 1 and the Promoter of Greb1 in TM4 Sertoli Cells.
abstract: Chromatin immunoprecipitation (ChIP) is a technique to investigate the interaction between proteins and DNA within the natural chromatin context of the cell. There are two previously identified two canonical estrogen response elements (ERE1 and ERE2) present in the 5'-flanking region of the Greb1 gene which is a known estrogen-responsive gene. ChIP results showed the physical interaction between estrogen receptor I (ESR1) and EREs in the Greb1 promoter in TM4 mouse Sertoli cells. This chapter describes the protocol for chromatin immunoprecipitation applied to the estrogen response elements in the Greb1 promoter.
authors.affiliation: Department of OB/GYN & Women's Health, University of Louisville School of Medicine, Louisville, KY, 40292, USA.
authors.forename: Jing
authors.initials: J
authors.lastname: Lin
authors.affiliation: Department of OB/GYN & Women's Health, University of Louisville School of Medicine, Louisville, KY, 40292, USA. zhenmin.lei@louisville.edu.|Department of OB/GYN & Women's Health, University of Louisville Health Sciences Center, 412 A Building, 500 South Preston Street, Louisville, KY, 40202, USA. zhenmin.lei@louisville.edu.
authors.forename: Zhenmin
authors.initials: Z
authors.lastname: Lei
mesh_terms: D024506:5' Flanking Region; D000818:Animals; D001665:Binding Sites; D002460:Cell Line; D047369:Chromatin Immunoprecipitation; D047628:Estrogen Receptor alpha / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D008297:Male; D008565:Membrane Proteins; D051379:Mice; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D020218:Response Elements; D012708:Sertoli Cells / Q000378:metabolism*; D057188:Workflow
chemical_list: D047628:Estrogen Receptor alpha; C404494:KIAA0575 protein, mouse; D008565:Membrane Proteins; D011506:Proteins
keywords: Chromatin immunoprecipitation; Estrogen receptor; Estrogen response element; Greb1; Sertoli cell