title: Stability of Curcuma longa rhizome lectin: Role of N-linked glycosylation.
abstract: Curcuma longa rhizome lectin, a mannose-binding protein of non-seed portions of turmeric, is known to have antifungal, antibacterial and α-glucosidase inhibitory activities. We studied the role of complex-type glycans attached to asparagine (Asn) 66 and Asn 110 to elucidate the role of carbohydrates in lectin activity and stability. Apart from the native lectin, the characteristics of a deglycosylated Escherichia coli expressed lectin, high-mannose oligosaccharides at both asparagines and its glycosylation mutants N66Q and N110Q expressed in Pichia pastoris, were compared to understand the relationship between glycosylation and activity. Far UV circular dichroism (CD) spectra, fluorescence emission maximum, hemagglutination assay show no change in secondary or tertiary structures or sugar-binding properties between wild-type and aforementioned recombinant lectins under physiological pH. But reduced agglutination activity and loss of tertiary structure are observed in the acidic pH range for the deglycosylated and the N110Q protein. In thermal and guanidine hydrochloride (GdnCl)-induced unfolding, the wild-type and high-mannose lectins possess higher stability compared with the deglycosylated recombinant lectin and both mutants, as measured by a higher Tm of denaturation or a greater free energy change, respectively. Reversibility experiments after thermal denaturation reveal that deglycosylated proteins tend to aggregate during thermal inactivation but the wild type shows a much greater recovery to the native state upon refolding. These results suggest that N-glycosylation in turmeric lectin is important for the maintenance of its proper folding upon changes in pH, and that the oligosaccharides help in maintaining the active conformation and prevent aggregation in unfolded or partially folded molecules.
authors.affiliation: Department of Biochemistry, Bose Institute, Calcutta 700054, India.
authors.forename: Himadri
authors.initials: H
authors.lastname: Biswas
authors.affiliation: Department of Biochemistry, Bose Institute, Calcutta 700054, India raja@jcbose.ac.in.
authors.forename: Rajagopal
authors.initials: R
authors.lastname: Chattopadhyaya
mesh_terms: D002241:Carbohydrates / Q000737:chemistry; D002942:Circular Dichroism; D030024:Curcuma / Q000737:chemistry*; D004926:Escherichia coli / Q000235:genetics; D005786:Gene Expression Regulation; D006031:Glycosylation; D037102:Lectins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D037601:Mannose-Binding Lectin / Q000737:chemistry* / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism; D027343:Rhizome / Q000737:chemistry*
chemical_list: D002241:Carbohydrates; D037102:Lectins; D037601:Mannose-Binding Lectin; D011134:Polysaccharides
keywords: GC–MS; MALDI-MS; agglutination; cloning; unfolding