title: ERIC-PCR Genotyping of Some Campylobacter jejuni Isolates of Chicken and Human Origin in Egypt.
abstract: The public health importance of the genus Campylobacter is attributed to several species causing diarrhea in consumers. Poultry and their meat are considered the most important sources of human campylobacteriosis. In this study, 287 samples from chicken (131 cloacal swabs, 39 chicken skin, 78 chicken meat, and 39 cecal parts) obtained from retail outlets as well as 246 stool swabs from gastroenteritis patients were examined. A representative number of the biochemically identified Campylobacter jejuni isolates were identified by real-time PCR, confirming the identification of the isolates as C. jejuni. Genotyping of the examined isolates (n = 31) by enterobacterial repetitive intergenic consensus PCR (ERIC-PCR) revealed a high discriminatory index of ERIC-PCR (D = 0.948), dividing C. jejuni isolates of chicken and human origins into 18 profiles and four clusters. The 18 profiles obtained indicated the heterogeneity of C. jejuni. Dendrogram analysis showed that four clusters were generated; all human isolates fell into clusters I and III. These observations further support the existence of a genetic relationship between human and poultry isolates examined in the present study. In conclusion, the results obtained support the speculation that poultry and poultry meat have an important role as sources of infection in the acquisition of Campylobacter infection in humans.
authors.affiliation: 1 Zoonoses Department, Faculty of Veterinary Medicine, Zagazig University , Zagazig, Egypt .
authors.forename: Heba A
authors.initials: HA
authors.lastname: Ahmed
authors.affiliation: 2 Bacteriology, Immunology, and Mycology Department, Faculty of Veterinary Medicine, Benha University , Banha, Egypt.
authors.forename: Fatma I
authors.initials: FI
authors.lastname: El Hofy
authors.affiliation: 3 Bacteriology, Immunology, and Mycology Department, Faculty of Veterinary Medicine, Zagazig University , Zagazig, Egypt .
authors.forename: Ahmed M
authors.initials: AM
authors.lastname: Ammar
authors.affiliation: 2 Bacteriology, Immunology, and Mycology Department, Faculty of Veterinary Medicine, Benha University , Banha, Egypt.
authors.forename: Ashraf A
authors.initials: AA
authors.lastname: Abd El Tawab
authors.affiliation: 4 Veterinary Hospital, Faculty of Veterinary Medicine, Zagazig University , Zagazig, Egypt .
authors.forename: Ahmed A
authors.initials: AA
authors.lastname: Hefny
mesh_terms: D000818:Animals; D002169:Campylobacter Infections / Q000453:epidemiology / Q000382:microbiology*; D016123:Campylobacter jejuni / Q000235:genetics* / Q000302:isolation & purification; D002645:Chickens / Q000382:microbiology*; D016000:Cluster Analysis; D016384:Consensus Sequence / Q000235:genetics; D021901:DNA, Intergenic / Q000235:genetics; D004534:Egypt / Q000453:epidemiology; D004755:Enterobacteriaceae / Q000235:genetics; D005838:Genotype; D006801:Humans; D011200:Poultry / Q000382:microbiology; D011201:Poultry Diseases / Q000453:epidemiology / Q000382:microbiology*; D060888:Real-Time Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D015047:Zoonoses
chemical_list: D021901:DNA, Intergenic
keywords: Campylobacter jejuni; Chicken; ERIC-PCR; Egypt; Genotyping; Human