title: Phosphopeptide Enrichment Using Various Magnetic Nanocomposites: An Overview.
abstract: Magnetic nanocomposites are hybrid structures consisting of an iron oxide (Fe3O4/γ-Fe2O3) superparamagnetic core and a coating shell which presents affinity for a specific target molecule. Within the scope of phosphopeptide enrichment, the magnetic core is usually first functionalized with an intermediate layer of silica or carbon to improve dispersibility and increase specific area, and then with an outer layer of a phosphate-affinity material. Fe3O4-coating materials include metal oxides, rare earth metal-based compounds, immobilized-metal ions, polymers, and many others. This chapter provides a generic overview of the different materials that can be found in literature and their advantages and drawbacks.
authors.affiliation: UCIBIO, REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516, Caparica, Portugal. ib348@cam.ac.uk.|Department of Chemical Engineering and Biotechnology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QT, UK. ib348@cam.ac.uk.
authors.forename: Íris L
authors.initials: ÍL
authors.lastname: Batalha
authors.affiliation: UCIBIO, REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516, Caparica, Portugal.
authors.forename: Ana Cecília A
authors.initials: AC
authors.lastname: Roque
mesh_terms: D000537:Aluminum Oxide / Q000737:chemistry; D000818:Animals; D052203:Ferrosoferric Oxide / Q000737:chemistry; D006801:Humans; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology; D010449:Peptide Mapping; D010748:Phosphopeptides / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D010766:Phosphorylation; D011108:Polymers / Q000737:chemistry; D011499:Protein Processing, Post-Translational; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014025:Titanium / Q000737:chemistry; D015040:Zirconium / Q000737:chemistry
chemical_list: D058185:Magnetite Nanoparticles; D010748:Phosphopeptides; D011108:Polymers; C009495:titanium dioxide; D015040:Zirconium; D014025:Titanium; D000537:Aluminum Oxide; C028541:zirconium oxide; D052203:Ferrosoferric Oxide
keywords: Affinity; Hybrid material s; Magnetic nanocomposite s; Phosphopeptide enrich ment