title: H3S10 phosphorylation-mediated transcriptional regulation by Aurora kinase A.
abstract: Histone H3S10 phosphorylation has been known as a cell cycle-specific marker and has a role in transcriptional activation. Various kinases phosphorylate H3S10 in different species, however, the role of the mitotic serine/threonine protein kinase Aurora A (AURKA) is largely unknown. Here we present evidence that AURKA phosphorylates H3S10 and activates target gene transcription. We show that down-regulation of AURKA level during leukemia cell differentiation results in decreased H3S10 phosphorylation level. We further show that AURKA is recruited to target gene promoters and activates transcription via H3S10 phosphorylation. Furthermore, this recruitment can be disrupted by the AURKA inhibitor Alisertib and results in H3K9-me2 recruitment by G9a.
authors.affiliation: Department of Life Science, College of Natural Sciences, Chung-Ang University, Seoul 06974, South Korea.
authors.forename: Se-Ryeon
authors.initials: SR
authors.lastname: Kim
authors.affiliation: Department of Life Science, College of Natural Sciences, Chung-Ang University, Seoul 06974, South Korea.
authors.forename: Kee-Beom
authors.initials: KB
authors.lastname: Kim
authors.affiliation: Department of Life Science, College of Natural Sciences, Chung-Ang University, Seoul 06974, South Korea.
authors.forename: Yun-Cheol
authors.initials: YC
authors.lastname: Chae
authors.affiliation: Department of Life Science, College of Natural Sciences, Chung-Ang University, Seoul 06974, South Korea.
authors.forename: Jin Woo
authors.initials: JW
authors.lastname: Park
authors.affiliation: Department of Life Science, College of Natural Sciences, Chung-Ang University, Seoul 06974, South Korea. Electronic address: sangbs@cau.ac.kr.
authors.forename: Sang-Beom
authors.initials: SB
authors.lastname: Seo
mesh_terms: D064096:Aurora Kinase A / Q000235:genetics*; D045744:Cell Line, Tumor; D005786:Gene Expression Regulation / Q000235:genetics; D018922:HL-60 Cells; D042421:Histone Code / Q000235:genetics*; D006657:Histones / Q000235:genetics*; D006801:Humans; D009374:Neoplasms, Experimental / Q000235:genetics*; D010766:Phosphorylation; D011401:Promoter Regions, Genetic / Q000235:genetics*; D014158:Transcription, Genetic / Q000235:genetics; D015533:Transcriptional Activation / Q000235:genetics*
chemical_list: D006657:Histones; C576917:AURKA protein, human; D064096:Aurora Kinase A
keywords: Aurora kinase A; G9a; H3S10 phosphorylation; Leukemia cell differentiation; Transcription