title: Pathways related to PMA-differentiated THP1 human monocytic leukemia cells revealed by RNA-Seq.
abstract: Previous analyses have reported that the human monocytic cell line THP1 can be differentiated into cells with macrophage-like characteristics by phorbol 12-myristate 13-acetate (PMA). However, little is known about the mechanism responsible for regulating this differentiation process. Here, we performed high-throughput RNA-Seq analysis to investigate the genes differently expressed in THP1 cells treated with and without PMA and examined those that may be responsible for the PMA-induced differentiation of monocytes into macrophages. We found 3,000 genes to be differentially expressed after PMA treatment. Gene ontology analysis revealed that genes related to cellular processes and regulation of biological processes were significantly enriched. KEGG analysis also demonstrated that the differentially expressed genes (DEGs) were significantly enriched in the PI3K/AKT signaling pathway and phagosome pathway. Importantly, we reveal an important role of the PI3K/AKT pathway in PMA-induced THP1 cell differentiation. The identified DEGs and pathways may facilitate further study of the detailed molecular mechanisms of THP1 differentiation. Thus, our results provide numerous potential therapeutic targets for modulation of the differentiation of this disease.
authors.affiliation: First Affiliated Hospital, Jinan University, Guangzhou, 510632, China.|Institute of Hematology, Medical College, Jinan University, Guangzhou, 510632, China.|Key Laboratory for Regenerative Medicine of Ministry of Education, Jinan University, Guangzhou, 510632, China.
authors.forename: ChengWu
authors.initials: C
authors.lastname: Zeng
authors.affiliation: Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory for Biocontrol, School of Life Science, Sun Yat-sen University, Guangzhou, 510275, China.
authors.forename: WenTao
authors.initials: W
authors.lastname: Wang
authors.affiliation: Key Laboratory for Regenerative Medicine of Ministry of Education, Jinan University, Guangzhou, 510632, China.
authors.forename: XiBao
authors.initials: X
authors.lastname: Yu
authors.affiliation: Institute of Hematology, Medical College, Jinan University, Guangzhou, 510632, China.
authors.forename: LiJian
authors.initials: L
authors.lastname: Yang
authors.affiliation: Institute of Hematology, Medical College, Jinan University, Guangzhou, 510632, China.
authors.forename: ShaoHua
authors.initials: S
authors.lastname: Chen
authors.affiliation: First Affiliated Hospital, Jinan University, Guangzhou, 510632, China. yangqiuli@hotmail.com.|Institute of Hematology, Medical College, Jinan University, Guangzhou, 510632, China. yangqiuli@hotmail.com.|Key Laboratory for Regenerative Medicine of Ministry of Education, Jinan University, Guangzhou, 510632, China. yangqiuli@hotmail.com.
authors.forename: YangQiu
authors.initials: Y
authors.lastname: Li
mesh_terms: D002454:Cell Differentiation / Q000235:genetics*; D045744:Cell Line, Tumor; D016000:Cluster Analysis; D020869:Gene Expression Profiling / Q000379:methods; D063990:Gene Ontology; D006801:Humans; D007938:Leukemia / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D009000:Monocytes / Q000187:drug effects* / Q000378:metabolism; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D017423:Sequence Analysis, RNA / Q000379:methods*; D015398:Signal Transduction / Q000235:genetics*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D059467:Transcriptome / Q000187:drug effects / Q000235:genetics
chemical_list: D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; D013755:Tetradecanoylphorbol Acetate
keywords: PI3K/AKT pathway; RNA sequencing; acute myeloid leukemia; differentiation; macrophage