title: Combined platelet and plasma derivatives enhance proliferation of stem/progenitor cells maintaining their differentiation potential.
abstract: OBJECTIVE
  Platelet derivatives have been proposed as alternatives to animal sera given that for cell therapy applications, the use of fetal bovine/calf serum (FBS/FCS) is subjected to severe limitations for safety and ethical concerns. We developed a cell culture medium additive obtained by the combination of two blood-derived standardized components.
  
  
  METHODS
  A platelet lysate (PL) and a platelet-poor plasma (PPP) were produced in a lyophilized form. Each component was characterized for its growth factor content (platelet-derived growth factor-BB/vascular endothelial growth factor). PL and PPP were used as single components or in combination in different ratio at cumulative 5% final concentration in the culture medium.
  
  
  RESULTS
  The single components were less effective than the component combination. In primary cell cultures (bone marrow stromal cells, adipose derived adult stem cells, osteoblasts, chondrocytes, umbilical cord-derived mesenchymal stromal cells, lymphocytes), the PL/PPP supplement promoted an increased cell proliferation in respect to the standard FCS culture in a dose-dependent manner, maintaining the cell functionality, clonogenicity, phenotype and differentiative properties throughout the culture. At a different component ratio, the supplement was also used to support proliferation of a cell line (U-937).
  
  
  CONCLUSIONS
  The PL/PPP supplement is an efficient cell culture medium additive that can replace FCS to promote cell proliferation. It can outdo FCS, especially when adopted in primary cultures from tissue biopsies. Moreover, the dual component nature of the supplement allows the researcher to determine the more appropriate ratio of the two components for the nutritional and functional requirements of the cell type of interest.
authors.affiliation: Biorigen S.r.l., Genoa, Italy.
authors.forename: Anita
authors.initials: A
authors.lastname: Muraglia
authors.affiliation: Biorigen S.r.l., Genoa, Italy; DIMES, University of Genoa, Genoa, Italy.
authors.forename: Maria Rosa
authors.initials: MR
authors.lastname: Todeschi
authors.affiliation: DIMES, University of Genoa, Genoa, Italy; IRCCS AOU San Martino-IST Istituto Nazionale per la Ricerca sul Cancro, Genoa, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Papait
authors.affiliation: IRCCS AOU San Martino-IST Istituto Nazionale per la Ricerca sul Cancro, Genoa, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Poggi
authors.affiliation: DIMES, University of Genoa, Genoa, Italy; IRCCS AOU San Martino-IST Istituto Nazionale per la Ricerca sul Cancro, Genoa, Italy.
authors.forename: Raffaele
authors.initials: R
authors.lastname: Spanò
authors.affiliation: IRCCS AOU San Martino-IST Istituto Nazionale per la Ricerca sul Cancro, Genoa, Italy.
authors.forename: Paolo
authors.initials: P
authors.lastname: Strada
authors.affiliation: DIMES, University of Genoa, Genoa, Italy; IRCCS AOU San Martino-IST Istituto Nazionale per la Ricerca sul Cancro, Genoa, Italy.
authors.forename: Ranieri
authors.initials: R
authors.lastname: Cancedda
authors.affiliation: DIMES, University of Genoa, Genoa, Italy; IRCCS AOU San Martino-IST Istituto Nazionale per la Ricerca sul Cancro, Genoa, Italy. Electronic address: maddalena.mastrogiacomo@unige.it.
authors.forename: Maddalena
authors.initials: M
authors.lastname: Mastrogiacomo
mesh_terms: D000328:Adult; D053687:Adult Stem Cells / Q000378:metabolism; D000818:Animals; D000077214:Becaplermin; D001792:Blood Platelets / Q000378:metabolism*; D002417:Cattle; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002457:Cell Extracts / Q000494:pharmacology; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D019902:Chondrocytes / Q000378:metabolism; D016895:Culture Media, Serum-Free / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010006:Osteoblasts / Q000378:metabolism; D010949:Plasma / Q000166:cytology; D020574:Proto-Oncogene Proteins c-sis / Q000378:metabolism; D013601:T-Lymphocytes / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D002457:Cell Extracts; D016895:Culture Media, Serum-Free; D020574:Proto-Oncogene Proteins c-sis; D042461:Vascular Endothelial Growth Factor A; D000077214:Becaplermin
keywords: differentiation; fetal calf serum; platelet lysate; platelet-poor plasma; proliferation; stem progenitor cells