title: New ethanol extraction improves the anti-obesity effects of black tea.
abstract: Black tea has been reported to have anti-obesity effects in both rodents and humans. Gallic acid, an active component of black tea, decomposes quickly into pyrogallol in high-temperature solutions. This study introduced a new, aqueous ethanol extraction of black tea, which resulted in extracts with higher concentrations of gallic acid than conventional black tea extracts prepared by hot-water extraction or hot-ethanol extraction. We confirmed that, compared with the hot-water extract of black tea, the cold-ethanol extract of black tea (CE-BTE) had greater effects on reducing body weight and body fat, improving fatty liver, regulating blood glucose, and reducing blood cholesterol in the high-fat diet-induced obese mouse model. Nonetheless, although CE-BTE significantly reduced fat content, it did not reduce peroxisome proliferator-activated receptor (PPARγ) protein in epididymal fat tissue of HFD mice. We also showed that CE-BTE did not inhibit the function of PPARγ protein to drive adipogenesis of mouse 3T3-L1 preadipocytes. Considering that PPARγ is a master transcription factor not only for adipocyte differentiation, but also for adipose tissue function, such as glucose and lipid metabolism and insulin sensitivity, these results suggest that CE-BTE reduced fat mass and body weight without dampening fat cell homeostasis and insulin sensitivity.
authors.affiliation: Department of Life Science, University of Seoul, Siripdae-gil 13, Dongdaemun-gu, Seoul, 130-743, Korea.
authors.forename: Bongju
authors.initials: B
authors.lastname: Park
authors.affiliation: Research Institute, Dong-A ST Co., Ltd., Gyeonggi, 446-905, Korea.|School of Pharmacy, Sungkyunkwan University, Suwon, 440-746, Korea.
authors.forename: Sangjin
authors.initials: S
authors.lastname: Lee
authors.affiliation: Research Institute, Dong-A ST Co., Ltd., Gyeonggi, 446-905, Korea.
authors.forename: Bonggyeong
authors.initials: B
authors.lastname: Lee
authors.affiliation: Department of Life Science, University of Seoul, Siripdae-gil 13, Dongdaemun-gu, Seoul, 130-743, Korea.
authors.forename: Ingyum
authors.initials: I
authors.lastname: Kim
authors.affiliation: Research Institute, Dong-A ST Co., Ltd., Gyeonggi, 446-905, Korea.
authors.forename: Namjoon
authors.initials: N
authors.lastname: Baek
authors.affiliation: Research Institute, Dong-A ST Co., Ltd., Gyeonggi, 446-905, Korea.
authors.forename: Tae Ho
authors.initials: TH
authors.lastname: Lee
authors.affiliation: School of Pharmacy, Sungkyunkwan University, Suwon, 440-746, Korea.
authors.forename: Seok-Yong
authors.initials: SY
authors.lastname: Lee
authors.affiliation: Research Institute, Dong-A ST Co., Ltd., Gyeonggi, 446-905, Korea. mwson@donga.co.kr.
authors.forename: Miwon
authors.initials: M
authors.lastname: Son
authors.affiliation: Department of Life Science, University of Seoul, Siripdae-gil 13, Dongdaemun-gu, Seoul, 130-743, Korea. hspark@uos.ac.kr.
authors.forename: Hyunsung
authors.initials: H
authors.lastname: Park
mesh_terms: D050153:Abdominal Fat / Q000187:drug effects; D017667:Adipocytes / Q000187:drug effects / Q000502:physiology; D000818:Animals; D019440:Anti-Obesity Agents / Q000737:chemistry / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D001835:Body Weight / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D059305:Diet, High-Fat; D004822:Epididymis / Q000378:metabolism; D000431:Ethanol / Q000737:chemistry*; D008099:Liver / Q000033:anatomy & histology / Q000187:drug effects; D008297:Male; D051379:Mice; D009765:Obesity / Q000139:chemically induced / Q000517:prevention & control; D047495:PPAR gamma / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D013662:Tea / Q000737:chemistry*; D014280:Triglycerides / Q000378:metabolism
chemical_list: D019440:Anti-Obesity Agents; D047495:PPAR gamma; D010936:Plant Extracts; D013662:Tea; D014280:Triglycerides; D000431:Ethanol
keywords: Adipogenesis; Anti-obesity; Black tea; PPARγ