title: Development, characterization and optimization of a new suspension chicken-induced pluripotent cell line for the production of Newcastle disease vaccine.
abstract: Traditionally, substrates for production of viral poultry vaccines have been embryonated eggs or adherent primary cell cultures. The difficulties and cost involved in scaling up these substrates in cases of increased demand have been a limitation for vaccine production. Here, we assess the ability of a newly developed chicken-induced pluripotent cell line, BA3, to support replication and growth of Newcastle disease virus (NDV) LaSota vaccine strain. The characteristics and growth profile of the cells were also investigated. BA3 cells could grow in suspension in different media to a high density of up to 7.0 × 10(6) cells/mL and showed rapid proliferation with doubling time of 21 h. Upon infection, a high virus titer of 1.02 × 10(8) EID50/mL was obtained at 24 h post infection using a multiplicity of infection (MOI) of 5. In addition, the cell line was shown to be free of endogenous and exogenous Avian Leukosis viruses, Reticuloendotheliosis virus, Fowl Adenovirus, Marek's disease virus, and several Mycoplasma species. In conclusion, BA3 cell line is potentially an excellent candidate for vaccine production due to its highly desirable industrially friendly characteristics of growing to high cell density and capability of growth in serum free medium.
authors.affiliation: Exotic and Emerging Avian Viral Diseases Research Unit, Southeast Poultry Research Laboratory, Athens, GA 30605, USA.
authors.forename: Ismaila
authors.initials: I
authors.lastname: Shittu
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Animal Reproduction Institute, Guangxi University, Nanning, Guangxi, China; Regenerative Bioscience Center, University of Georgia, Athens, GA 30602, USA; Department of Animal and Dairy Science, University of Georgia, Athens, GA 30602, USA.
authors.forename: Ziying
authors.initials: Z
authors.lastname: Zhu
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Animal Reproduction Institute, Guangxi University, Nanning, Guangxi, China; Regenerative Bioscience Center, University of Georgia, Athens, GA 30602, USA; Department of Animal and Dairy Science, University of Georgia, Athens, GA 30602, USA.
authors.forename: Yangqing
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Regenerative Bioscience Center, University of Georgia, Athens, GA 30602, USA; Department of Animal and Dairy Science, University of Georgia, Athens, GA 30602, USA.
authors.forename: Jessica M
authors.initials: JM
authors.lastname: Hutcheson
authors.affiliation: Regenerative Bioscience Center, University of Georgia, Athens, GA 30602, USA; Department of Animal and Dairy Science, University of Georgia, Athens, GA 30602, USA.
authors.forename: Steven L
authors.initials: SL
authors.lastname: Stice
authors.affiliation: Regenerative Bioscience Center, University of Georgia, Athens, GA 30602, USA; Department of Animal and Dairy Science, University of Georgia, Athens, GA 30602, USA.
authors.forename: Franklin D
authors.initials: FD
authors.lastname: West
authors.affiliation: GALVmed, Pentlands Science Park, Edinburgh EH26 0PZ, UK.
authors.forename: Meritxell
authors.initials: M
authors.lastname: Donadeu
authors.affiliation: GALVmed, Pentlands Science Park, Edinburgh EH26 0PZ, UK.
authors.forename: Baptiste
authors.initials: B
authors.lastname: Dungu
authors.affiliation: Avian Disease and Oncology Laboratory, U.S. Department of Agriculture, Agricultural Research Service, 4279 East Mount Hope Road, East Lansing, MI 48823, USA.
authors.forename: Aly M
authors.initials: AM
authors.lastname: Fadly
authors.affiliation: Poultry Diagnostic and Research Center, Department of Population Health, University of Georgia, Athens, GA 30602, USA.
authors.forename: Guillermo
authors.initials: G
authors.lastname: Zavala
authors.affiliation: Poultry Diagnostic and Research Center, Department of Population Health, University of Georgia, Athens, GA 30602, USA.
authors.forename: Naola
authors.initials: N
authors.lastname: Ferguson-Noel
authors.affiliation: Exotic and Emerging Avian Viral Diseases Research Unit, Southeast Poultry Research Laboratory, Athens, GA 30605, USA. Electronic address: claudio.afonso@ars.usda.gov.
authors.forename: Claudio L
authors.initials: CL
authors.lastname: Afonso
mesh_terms: D000818:Animals; D002460:Cell Line; D002642:Chick Embryo; D002645:Chickens; D057026:Induced Pluripotent Stem Cells / Q000378:metabolism / Q000821:virology; D009521:Newcastle Disease / Q000517:prevention & control*; D009522:Newcastle disease virus; D014765:Viral Vaccines / Q000096:biosynthesis*
chemical_list: D014765:Viral Vaccines
keywords: Chicken-induced pluripotent cell; Newcastle disease virus; Serum free medium; Suspension cell culture; Vaccine production