title: Genetic engineering of Ganoderma lucidum for the efficient production of ganoderic acids.
abstract: Ganoderma lucidum is a well-known traditional medicinal mushroom that produces ganoderic acids with numerous interesting bioactivities. Genetic engineering is an efficient approach to improve ganoderic acid biosynthesis. However, reliable genetic transformation methods and appropriate genetic manipulation strategies remain underdeveloped and thus should be enhanced. We previously established a homologous genetic transformation method for G. lucidum; we also applied the established method to perform the deregulated overexpression of a homologous 3-hydroxy-3-methyl-glutaryl coenzyme A reductase gene in G. lucidum. Engineered strains accumulated more ganoderic acids than wild-type strains. In this report, the genetic transformation systems of G. lucidum are described; current trends are also presented to improve ganoderic acid production through the genetic manipulation of G. lucidum.
authors.affiliation: a Faculty of Life Science and Technology; Kunming University of Science and Technology ; Kunming , China.
authors.forename: Jun-Wei
authors.initials: JW
authors.lastname: Xu
authors.affiliation: b State Key Lab. of Microbial Metabolism; Joint International Research Laboratory of Metabolic & Developmental Sciences and School of Life Sciences & Biotechnology; Shanghai Jiao Tong University ; Shanghai , China.
authors.forename: Jian-Jiang
authors.initials: JJ
authors.lastname: Zhong
mesh_terms: D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D005800:Genes, Fungal; D005818:Genetic Engineering / Q000379:methods*; D006903:Hydroxymethylglutaryl CoA Reductases / Q000235:genetics / Q000378:metabolism; D008516:Medicine, Chinese Traditional; D060847:Metabolic Engineering / Q000379:methods; D020940:Reishi / Q000235:genetics* / Q000378:metabolism*; D014170:Transformation, Genetic; D014315:Triterpenes / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D005656:Fungal Proteins; D014315:Triterpenes; C556862:ganoderic acid; D006903:Hydroxymethylglutaryl CoA Reductases
keywords: Ganoderm lucidum; ganoderic acid production; genetic engineering; genetic transformation; medicinal mushroom