title: Evaluation of anti-microbial activity of spore powder of Ganoderma lucidum on clinical isolates of Prevotella intermedia: A pilot study.
abstract: OBJECTIVE
  This study aimed at evaluating the anti-microbial activity of spore powder of Ganoderma lucidum on Prevotella intermedia isolated from subgingival plaque from chronic periodontitis patients.
  
  
  METHODS
  Written informed consent was obtained from each subject enrolled in the study. The Institutional Ethics Committee granted the ethical clearance for the study.
  
  
  METHODS
  This study included 20 patients diagnosed with chronic periodontitis. Pooled subgingival plaque samples were collected using sterile curettes from the deepest sites of periodontal pockets. The collected samples were then transported in 1 mL of reduced transport fluid. The organisms were cultured and confirmed. These organisms were then used for minimum inhibitory concentration (MIC) procedure.
  
  
  METHODS
  Mean of the MIC value obtained was calculated.
  
  
  RESULTS
  Thirteen out of the 20 clinical samples were tested that showed sensitivity at various concentrations. Five samples showed sensitivity at all concentrations. Twelve samples showed sensitivity at 8 mcg/ml. Eleven samples showed sensitivity at 4 mcg/ml, 8 samples showed sensitivity at 2 mcg/ml, and 5 samples showed sensitivity even at 1 mcg/ml. Mean MIC value of G. lucidum spore powder for P. intermedia obtained was 3.62 mcg/ml.
  
  
  CONCLUSIONS
  G. lucidum with its multipotential bioactivity could be used as an anti-microbial, in conjunction with conventional therapy in periodontal disease.
authors.affiliation: Department of Periodontology, Maratha Mandal's Nathajirao G. Halgekar Institute of Dental Sciences and Research Center, Belgaum, Karnataka, India.
authors.forename: Ranganath N
authors.initials: RN
authors.lastname: Nayak
authors.affiliation: Department of Periodontology, Maratha Mandal's Nathajirao G. Halgekar Institute of Dental Sciences and Research Center, Belgaum, Karnataka, India.
authors.forename: P T
authors.initials: PT
authors.lastname: Dixitraj
authors.affiliation: Department of Periodontology, Maratha Mandal's Nathajirao G. Halgekar Institute of Dental Sciences and Research Center, Belgaum, Karnataka, India.
authors.forename: Aarati
authors.initials: A
authors.lastname: Nayak
authors.affiliation: Department of Periodontology, Maratha Mandal's Nathajirao G. Halgekar Institute of Dental Sciences and Research Center, Belgaum, Karnataka, India.
authors.forename: Kishore
authors.initials: K
authors.lastname: Bhat
keywords: Anaerobic bacteria; Ganoderma lucidum; Gram-negative bacteria; Prevotella intermedia; anti-microbial agents; periodontitis