title: Construction of Plasmonic Core-Satellite Nanostructures on Substrates Based on DNA-Directed Self-Assembly as a Sensitive and Reproducible Biosensor.
abstract: We report the successful construction of plasmonic core-satellite nanostructured assemblies on two-dimensional substrates, based on a strategy of combining DNA-functionalized plasmonic nanoparticles (NPs) with the specific recognition ability toward target to enable satellite NPs to self-assemble around the core immobilized on substrates. A strongly coupled plasmonic resonance band was observed because of the close proximity between core and satellite NPs, which presented significant red-shift and enhanced extinction with respect to the local surface plasmon resonance (LSPR) band of individual core NPs on the substrate. The functionality of this core-satellite nanostructured assembly as a biosensor was further explored, and the changes in extinction intensity and the peak shift of the plasmonic coupling resonance band arising from the probe-target DNA binding event all proved to be useful criteria for target DNA detection. Moreover, high selectivity down to single-base mismatched DNA was achieved using this strongly coupled plasmonic core-satellite nanostructured assembly on a substrate. Such substrate-based detection was advantageous, and its reusability and high cycle stability were demonstrated after five cycles of disassembly and reassembly. Our work demonstrates the biosensing capacity of this DNA-functionalized plasmonic nanoassembly model system on two-dimensional substrate, which is also applicable to the detection of numerous DNA-recognized biomolecules. Likewise, the presented construction method can be extended to fabricate other compositional core-satellite nanoassemblies.
authors.affiliation: State Key Laboratory of Chemical Resource Engineering & Key Laboratory of Carbon Fiber and Functional Polymers, Ministry of Education, College of Materials Science & Engineering, Beijing University of Chemical Technology , Beijing, 100029, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Chemical Resource Engineering & Key Laboratory of Carbon Fiber and Functional Polymers, Ministry of Education, College of Materials Science & Engineering, Beijing University of Chemical Technology , Beijing, 100029, China.
authors.forename: He
authors.initials: H
authors.lastname: Li
authors.affiliation: State Key Laboratory of Chemical Resource Engineering & Key Laboratory of Carbon Fiber and Functional Polymers, Ministry of Education, College of Materials Science & Engineering, Beijing University of Chemical Technology , Beijing, 100029, China.
authors.forename: Shengwei
authors.initials: S
authors.lastname: Hou
authors.affiliation: College of Chemistry and Materials Engineering, Wenzhou University , Wenzhou, 325027, China.
authors.forename: Youqing
authors.initials: Y
authors.lastname: Dong
authors.affiliation: State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, College of Chemistry, Jilin University , Changchun, 130012, China.
authors.forename: Guangsheng
authors.initials: G
authors.lastname: Pang
authors.affiliation: State Key Laboratory of Chemical Resource Engineering & Key Laboratory of Carbon Fiber and Functional Polymers, Ministry of Education, College of Materials Science & Engineering, Beijing University of Chemical Technology , Beijing, 100029, China.
authors.forename: Yingwei
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry*; D020349:Surface Plasmon Resonance
chemical_list: D004247:DNA
keywords: biosensor; core−satellite nanostructure; local surface plasmon resonance; plasmon coupling; reproducibility; self-assembly