title: Adenylate cyclase toxin-mediated delivery of the S1 subunit of pertussis toxin into mammalian cells.
abstract: The adenylate cyclase toxin (ACT) of Bordetella pertussis internalizes its catalytic domain into target cells. ACT can function as a tool for delivering foreign protein antigen moieties into immune effector cells to induce a cytotoxic T lymphocyte response. In this study, we replaced the catalytic domain of ACT with an enzymatically active protein moiety, the S1 (ADP-ribosyltransferase) subunit of pertussis toxin (PT). The S1 moiety was successfully internalized independent of endocytosis into sheep erythrocytes. The introduced polypeptide exhibited ADP-ribosyltransferase activity in CHO cells and induced clustering typical to PT. The results indicate that ACT can act as a vehicle for not only epitopes but also enzymatically active peptides to mammalian cells.
authors.affiliation: Department of Bacteriology II, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama-shi, Tokyo 208-0011, Japan miwaki@nih.go.jp.
authors.forename: Masaaki
authors.initials: M
authors.lastname: Iwaki
authors.affiliation: Department of Bacteriology II, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama-shi, Tokyo 208-0011, Japan.
authors.forename: Toshifumi
authors.initials: T
authors.lastname: Konda
mesh_terms: D037361:Adenylate Cyclase Toxin / Q000378:metabolism*; D000818:Animals; D001886:Bordetella pertussis / Q000378:metabolism*; D016466:CHO Cells; D003412:Cricetulus; D004847:Epithelial Cells / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism*; D037342:Pertussis Toxin / Q000378:metabolism*; D021381:Protein Transport; D012756:Sheep
chemical_list: D037361:Adenylate Cyclase Toxin; C083388:pertussis toxin, S1 subunit; D037342:Pertussis Toxin
keywords: ADP-ribosylation; Bordetella pertussis; CHO cell clustering; CyaA; cell invasion; sheep erythrocytes