title: Secretome profiling of cytokines and growth factors reveals that neuro-glial differentiation is associated with the down-regulation of Chemokine Ligand 2 (MCP-1/CCL2) in amniotic fluid derived-mesenchymal progenitor cells.
abstract: Secreted cytokines and growth factors play a key role in the modulation of stem cell proliferation, differentiation and survival. To investigate the interplay between the changes in their expression levels, we used the newly characterized human amniotic fluid derived-mesenchymal progenitor MePR-2B cell line differentiated to a neuro-glial phenotype and exploited the very high sensitivity and versatility of magnetic beads-based immunoassays. We found that a sub-set of proteins, including the cytokines IL-6, TNFα, IL-15, IFNγ, IL-8, IL-1ra, MCP-1/CCL2, RANTES and the growth factor PDGFbb, underwent a significant down-regulation following neuro-glial differentiation, whereas the expression levels of IL-12 p70, IL-5, IL-7, bFGF, VEGF and G-CSF were increased. The role of MCP-1/CCL2, previously identified as a regulator of neural progenitor stem cell differentiation, has been further investigated at transcriptional level, revealing that both the chemokine and its receptor are co-expressed in MePR-2B cells and that are regulated upon differentiation, suggesting the presence of an autocrine and paracrine loop in differentiating cells. Moreover, we demonstrated that exogenous CCL2 is capable to affect neuro-glial differentiation in MePR-2B cells, thus providing novel evidences for the potential involvement of chemokine-mediated signaling in progenitor/stem cells differentiation processes and fate specification.
authors.affiliation: Dipartimento di Biochimica, Biofisica e Patologia Generale, Seconda Università di Napoli, Napoli, Italy.|Istituto di Genetica e Biofisica 'Adriano Buzzati Traverso' IGB, CNR, Napoli, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Miceli
authors.affiliation: Dipartimento di Biochimica, Biofisica e Patologia Generale, Seconda Università di Napoli, Napoli, Italy.|Istituto di Genetica e Biofisica 'Adriano Buzzati Traverso' IGB, CNR, Napoli, Italy.
authors.forename: Carmela
authors.initials: C
authors.lastname: Dell'Aversana
authors.affiliation: Dipartimento di Scienze e Tecnologie Ambientali, Biologiche e Farmaceutiche, Seconda Università degli Studi di Napoli, Caserta, Italy.
authors.forename: Rosita
authors.initials: R
authors.lastname: Russo
authors.affiliation: Dipartimento di Scienze e Tecnologie Ambientali, Biologiche e Farmaceutiche, Seconda Università degli Studi di Napoli, Caserta, Italy.
authors.forename: Camilla
authors.initials: C
authors.lastname: Rega
authors.affiliation: Dipartimento di Biochimica, Biofisica e Patologia Generale, Seconda Università di Napoli, Napoli, Italy.|Istituto di Genetica e Biofisica 'Adriano Buzzati Traverso' IGB, CNR, Napoli, Italy.
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Cupelli
authors.affiliation: Istituto di Biostrutture e Bioimmagini, IBB, CNR, Napoli, Italy.
authors.forename: Menotti
authors.initials: M
authors.lastname: Ruvo
authors.affiliation: Dipartimento di Biochimica, Biofisica e Patologia Generale, Seconda Università di Napoli, Napoli, Italy.|Istituto di Genetica e Biofisica 'Adriano Buzzati Traverso' IGB, CNR, Napoli, Italy.
authors.forename: Lucia
authors.initials: L
authors.lastname: Altucci
authors.affiliation: Dipartimento di Scienze e Tecnologie Ambientali, Biologiche e Farmaceutiche, Seconda Università degli Studi di Napoli, Caserta, Italy.|IRCCS, Multimedica, Milano, Italy.
authors.forename: Angela
authors.initials: A
authors.lastname: Chambery
mesh_terms: D000653:Amniotic Fluid / Q000166:cytology*; D002454:Cell Differentiation; D002460:Cell Line; D018932:Chemokine CCL2 / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D016207:Cytokines / Q000032:analysis* / Q000378:metabolism; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000032:analysis* / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D009457:Neuroglia / Q000166:cytology* / Q000378:metabolism
chemical_list: D018932:Chemokine CCL2; D016207:Cytokines; D036341:Intercellular Signaling Peptides and Proteins
keywords: Biomedicine; Cytokines profiling; Multiplexed immunoassay; Neuro-glial differentiation; Secretome; Stem cells