title: Early differences in metabolic flexibility between obesity-resistant and obesity-prone mice.
abstract: Decreased metabolic flexibility, i.e. a compromised ability to adjust fuel oxidation to fuel availability supports development of adverse consequences of obesity. The aims of this study were (i) to learn whether obesity-resistant A/J and obesity-prone C57BL/6J mice differ in their metabolic flexibility right after weaning; and (ii) to characterize possible differences in control of glucose homeostasis in these animals using glucose tolerance tests (GTT). A/J and C57BL/6J mice of both genders were maintained at 20 °C and weaned to standard low-fat diet at 30 days of age. During the first day after weaning, using several separate animal cohorts, (i) GTT was performed using 1 or 3 mg glucose/g body weight (BW), while glucose was administered either orally (OGTT) or intraperitoneally (IPGTT) at 20 °C; and (ii) indirect calorimetry (INCA) was performed, either in a combination with oral gavage of 1 or 7.5 mg glucose/g BW, or during a fasting/re-feeding transition. INCA was conducted either at 20 °C or 34 °C. Results of both OGTT and IPGTT using 1 mg glucose/g BW at 20 °C, and INCA using 7.5 mg glucose/g BW at 34 °C, indicated higher glucose tolerance and higher metabolic flexibility to glucose, respectively, and lower fasting glycemia in A/J mice as compared with C57BL/6J mice. Thus, control of whole body glucose metabolism between A/J and C57BL/6J mice represents a phenotypic feature differentiating between the strains right after weaning.
authors.affiliation: Department of Adipose Tissue Biology, Institute of Physiology of the Czech Academy of Sciences, Videnska 1083, 142 20 Prague, Czech Republic. Electronic address: kristina.bardova@fgu.cas.cz.
authors.forename: Kristina
authors.initials: K
authors.lastname: Bardova
authors.affiliation: Department of Adipose Tissue Biology, Institute of Physiology of the Czech Academy of Sciences, Videnska 1083, 142 20 Prague, Czech Republic. Electronic address: olga.horakova@fgu.cas.cz.
authors.forename: Olga
authors.initials: O
authors.lastname: Horakova
authors.affiliation: Department of Adipose Tissue Biology, Institute of Physiology of the Czech Academy of Sciences, Videnska 1083, 142 20 Prague, Czech Republic. Electronic address: petra.janovska@fgu.cas.cz.
authors.forename: Petra
authors.initials: P
authors.lastname: Janovska
authors.affiliation: Department of Adipose Tissue Biology, Institute of Physiology of the Czech Academy of Sciences, Videnska 1083, 142 20 Prague, Czech Republic. Electronic address: jana.hansikova@fgu.cas.cz.
authors.forename: Jana
authors.initials: J
authors.lastname: Hansikova
authors.affiliation: Department of Adipose Tissue Biology, Institute of Physiology of the Czech Academy of Sciences, Videnska 1083, 142 20 Prague, Czech Republic. Electronic address: vladimir.kus1@gmail.com.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Kus
authors.affiliation: Human and Animal Physiology, Wageningen University, De Elst 1, 6708 WD Wageningen, The Netherlands. Electronic address: evert.vanschothorst@wur.nl.
authors.forename: Evert M
authors.initials: EM
authors.lastname: van Schothorst
authors.affiliation: Human and Animal Physiology, Wageningen University, De Elst 1, 6708 WD Wageningen, The Netherlands. Electronic address: hoevenaars@vumc.nl.
authors.forename: Femke P M
authors.initials: FPM
authors.lastname: Hoevenaars
authors.affiliation: Human and Animal Physiology, Wageningen University, De Elst 1, 6708 WD Wageningen, The Netherlands. Electronic address: melissa.uil@gmail.com.
authors.forename: Melissa
authors.initials: M
authors.lastname: Uil
authors.affiliation: Department of Adipose Tissue Biology, Institute of Physiology of the Czech Academy of Sciences, Videnska 1083, 142 20 Prague, Czech Republic. Electronic address: hensler.michal@gmail.com.
authors.forename: Michal
authors.initials: M
authors.lastname: Hensler
authors.affiliation: Human and Animal Physiology, Wageningen University, De Elst 1, 6708 WD Wageningen, The Netherlands. Electronic address: jaap.keijer@wur.nl.
authors.forename: Jaap
authors.initials: J
authors.lastname: Keijer
authors.affiliation: Department of Adipose Tissue Biology, Institute of Physiology of the Czech Academy of Sciences, Videnska 1083, 142 20 Prague, Czech Republic. Electronic address: kopecky@biomed.cas.cz.
authors.forename: Jan
authors.initials: J
authors.lastname: Kopecky
mesh_terms: D000818:Animals; D005260:Female; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology; D005951:Glucose Tolerance Test; D008297:Male; D051379:Mice; D009765:Obesity / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D013045:Species Specificity
chemical_list: D005947:Glucose
keywords: A/J mice; C57BL/6J mice; Glucose tolerance; Indirect calorimetry; Metabolic flexibility; Weaning