title: Interactions of two cytotoxic organoruthenium(II) complexes with G-quadruplex.
abstract: Two previously isolated cytotoxic complexes [(η(6)-p-cymene)Ru(κ(2)-CF3COCHCOC5H3O)L](n+) (L=Cl (1); n=0, pta (2) (pta=1,3,5-triaza-7-phosphaadamantane); n=1) were investigated for their selectivity and ability to interact with DNA G-quadruplex adopted by d[G3ATG3ACACAG4ACG3] (3) whose topology exhibits diagonal, edge-type and double-chain reversal loops. Structural changes were followed using high-resolution NMR techniques in the presence of 1 and 2. Results showed weak interaction between the organoruthenium complexes and G-quadruplex. Moreover, no significant changes in thermal stability were confirmed by a UV-melting assay for both 1 and 2. These findings emphasize that anticancer activity of Ru(II) complexes may not be correlated with binding to nucleic acid like G-quadruplex.
authors.affiliation: Faculty of Chemistry and Chemical Technology, University of Ljubljana, Večna pot 113, SI-1000 Ljubljana, Slovenia.
authors.forename: Sara
authors.initials: S
authors.lastname: Seršen
authors.affiliation: EN-FIST Center of Excellence, SI-1000 Ljubljana, Slovenia; Slovenian NMR Center, National Institute of Chemistry, Hajdrihova 19, SI-1000 Ljubljana, Slovenia.
authors.forename: Primož
authors.initials: P
authors.lastname: Šket
authors.affiliation: Faculty of Chemistry and Chemical Technology, University of Ljubljana, Večna pot 113, SI-1000 Ljubljana, Slovenia; EN-FIST Center of Excellence, SI-1000 Ljubljana, Slovenia; Slovenian NMR Center, National Institute of Chemistry, Hajdrihova 19, SI-1000 Ljubljana, Slovenia.
authors.forename: Janez
authors.initials: J
authors.lastname: Plavec
authors.affiliation: Faculty of Chemistry and Chemical Technology, University of Ljubljana, Večna pot 113, SI-1000 Ljubljana, Slovenia; EN-FIST Center of Excellence, SI-1000 Ljubljana, Slovenia. Electronic address: iztok.turel@fkkt.uni-lj.si.
authors.forename: Iztok
authors.initials: I
authors.lastname: Turel
mesh_terms: D000218:Adamantane / Q000031:analogs & derivatives* / Q000737:chemistry; D000970:Antineoplastic Agents / Q000737:chemistry*; D001665:Binding Sites; D056831:Coordination Complexes / Q000737:chemistry*; D018360:Crystallography, X-Ray; D000081007:Cymenes; D003903:Deuterium; D054856:G-Quadruplexes; D009682:Magnetic Resonance Spectroscopy; D008968:Molecular Conformation; D039821:Monoterpenes / Q000737:chemistry*; D009691:Nucleic Acid Denaturation; D009942:Organometallic Compounds / Q000737:chemistry*; D009943:Organophosphorus Compounds / Q000737:chemistry*; D012428:Ruthenium / Q000737:chemistry*; D014466:Ultraviolet Rays
chemical_list: C501763:1,3,5-triaza-7-phosphaadamantane; D000970:Antineoplastic Agents; D056831:Coordination Complexes; D000081007:Cymenes; D039821:Monoterpenes; D009942:Organometallic Compounds; D009943:Organophosphorus Compounds; C007210:4-cymene; D012428:Ruthenium; D003903:Deuterium; D000218:Adamantane
keywords: Anticancer activity; G-quadruplex; NMR study; Organoruthenium complex; UV melting