title: Identification and Functional Analysis of the Nocardithiocin Gene Cluster in Nocardia pseudobrasiliensis.
abstract: Nocardithiocin is a thiopeptide compound isolated from the opportunistic pathogen Nocardia pseudobrasiliensis. It shows a strong activity against acid-fast bacteria and is also active against rifampicin-resistant Mycobacterium tuberculosis. Here, we report the identification of the nocardithiocin gene cluster in N. pseudobrasiliensis IFM 0761 based on conserved thiopeptide biosynthesis gene sequence and the whole genome sequence. The predicted gene cluster was confirmed by gene disruption and complementation. As expected, strains containing the disrupted gene did not produce nocardithiocin while gene complementation restored nocardithiocin production in these strains. The predicted cluster was further analyzed using RNA-seq which showed that the nocardithiocin gene cluster contains 12 genes within a 15.2-kb region. This finding will promote the improvement of nocardithiocin productivity and its derivatives production.
authors.affiliation: Medical Mycology Research Center, Chiba University, Chiba, Japan.
authors.forename: Kanae
authors.initials: K
authors.lastname: Sakai
authors.affiliation: Biological Resource Center, National Institute of Technology and Evaluation, Chiba, Japan.
authors.forename: Hisayuki
authors.initials: H
authors.lastname: Komaki
authors.affiliation: Medical Mycology Research Center, Chiba University, Chiba, Japan.
authors.forename: Tohru
authors.initials: T
authors.lastname: Gonoi
mesh_terms: D000900:Anti-Bacterial Agents / Q000096:biosynthesis; D017931:DNA Primers; D005816:Genetic Complementation Test; D016680:Genome, Bacterial; D013058:Mass Spectrometry; D005810:Multigene Family; D009161:Mycobacterium / Q000187:drug effects; D009615:Nocardia / Q000235:genetics* / Q000378:metabolism; D010455:Peptides / Q000737:chemistry; D010456:Peptides, Cyclic / Q000235:genetics* / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D017423:Sequence Analysis, RNA; D013045:Species Specificity; D013056:Spectrophotometry, Ultraviolet; D013844:Thiazoles / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D017931:DNA Primers; D010455:Peptides; D010456:Peptides, Cyclic; D013844:Thiazoles; C545886:nocardithiocin