title: The impact of silica encapsulated cobalt zinc ferrite nanoparticles on DNA, lipids and proteins of rat bone marrow mesenchymal stem cells.
abstract: Nanomaterials are currently the subject of intense research due to their wide variety of potential applications in the biomedical, optical and electronic fields. We prepared and tested cobalt zinc ferrite nanoparticles (Co0.5Zn0.5Fe2O4+γ [CZF-NPs]) encapsulated by amorphous silica in order to find a safe contrast agent and magnetic label for tracking transplanted cells within an organism using magnetic resonance imaging (MRI). Rat mesenchymal stem cells (rMSCs) were labeled for 48 h with a low, medium or high dose of CZF-NPs (0.05; 0.11 or 0.55 mM); silica NPs (Si-NPs; 0.11 mM) served as a positive control. The internalization of NPs into cells was verified by transmission electron microscopy. Biological effects were analyzed at the end of exposure and after an additional 72 h of cell growth without NPs. Compared to untreated cells, Annexin V/Propidium Iodide labeling revealed no significant cytotoxicity for any group of treated cells and only a high dose of CZF-NPs slowed down cell proliferation and induced DNA damage, manifested as a significant increase of DNA-strand breaks and oxidized DNA bases. This was accompanied by high concentrations of 15-F2t-isoprostane and carbonyl groups, demonstrating oxidative injury to lipids and proteins, respectively. No harmful effects were detected in cells exposed to the low dose of CZF-NPs. Nevertheless, the labeled cells still exhibited an adequate relaxation rate for MRI in repeated experiments and ICP-MS confirmed sufficient magnetic label concentrations inside the cells. The results suggest that the silica-coated CZF-NPs, when applied at a non-toxic dose, represent a promising contrast agent for cell labeling.
authors.affiliation: a Department of Genetic Ecotoxicology , Institute of Experimental Medicine AS CR , v.v.i., Prague , Czech Republic .
authors.forename: Bozena
authors.initials: B
authors.lastname: Novotna
authors.affiliation: a Department of Genetic Ecotoxicology , Institute of Experimental Medicine AS CR , v.v.i., Prague , Czech Republic .
authors.forename: Karolina
authors.initials: K
authors.lastname: Turnovcova
authors.affiliation: b Institute of Physics, AS CR, v.v.i. , Prague , Czech Republic .
authors.forename: Pavel
authors.initials: P
authors.lastname: Veverka
authors.affiliation: a Department of Genetic Ecotoxicology , Institute of Experimental Medicine AS CR , v.v.i., Prague , Czech Republic .
authors.forename: Pavel
authors.initials: P
authors.lastname: Rössner
authors.affiliation: a Department of Genetic Ecotoxicology , Institute of Experimental Medicine AS CR , v.v.i., Prague , Czech Republic .
authors.forename: Yana
authors.initials: Y
authors.lastname: Bagryantseva
authors.affiliation: c Institute for Clinical and Experimental Medicine , Prague , Czech Republic .
authors.forename: Vit
authors.initials: V
authors.lastname: Herynek
authors.affiliation: d Zentiva, k.s. , Prague , Czech Republic .
authors.forename: Pavel
authors.initials: P
authors.lastname: Zvatora
authors.affiliation: e University of Chemistry and Technology , Prague , Czech Republic , and.
authors.forename: Magda
authors.initials: M
authors.lastname: Vosmanska
authors.affiliation: f New Technologies - Research Centre, University of West Bohemia , Pilsen , Czech Republic.
authors.forename: Mariana
authors.initials: M
authors.lastname: Klementova
authors.affiliation: a Department of Genetic Ecotoxicology , Institute of Experimental Medicine AS CR , v.v.i., Prague , Czech Republic .
authors.forename: Eva
authors.initials: E
authors.lastname: Sykova
authors.affiliation: a Department of Genetic Ecotoxicology , Institute of Experimental Medicine AS CR , v.v.i., Prague , Czech Republic .
authors.forename: Pavla
authors.initials: P
authors.lastname: Jendelova
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003035:Cobalt / Q000737:chemistry / Q000633:toxicity; D003287:Contrast Media / Q000737:chemistry / Q000633:toxicity*; D004249:DNA Damage; D015237:Dinoprost / Q000031:analogs & derivatives; D004305:Dose-Response Relationship, Drug; D005290:Ferric Compounds / Q000737:chemistry / Q000633:toxicity; D028421:Isoprostanes / Q000378:metabolism; D050356:Lipid Metabolism / Q000187:drug effects*; D008279:Magnetic Resonance Imaging; D059630:Mesenchymal Stem Cells / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry / Q000633:toxicity*; D050050:Protein Carbonylation / Q000187:drug effects*; D051381:Rats; D012822:Silicon Dioxide / Q000737:chemistry / Q000633:toxicity*; D013194:Staining and Labeling; D013499:Surface Properties; D017967:Zinc Compounds / Q000737:chemistry / Q000633:toxicity
chemical_list: D003287:Contrast Media; D005290:Ferric Compounds; D028421:Isoprostanes; D017967:Zinc Compounds; C075750:8-epi-prostaglandin F2alpha; D003035:Cobalt; D012822:Silicon Dioxide; D015237:Dinoprost
keywords: Comet assay; cytotoxicity; genotoxicity; lipid peroxidation; protein oxidative damage