title: Identification of novel putative adipomyokines by a cross-species annotation of secretomes and expression profiles.
abstract: Adipose tissue and skeletal muscle are organs that respond strongly to obesity and physical activity exhibiting high secretory activity. To identify novel putative adipomyokines, comparative expression studies of skeletal muscle and adipose tissue of lean (C57BL/6J) and obese (C57BL/6J on a high-fat diet and NZO) mice, of sedentary and endurance trained C57BL/6J mice and of cattle characterized by different amounts of intramuscular fat were combined with human secretome data and scored. In highly regulated transcripts, we identified 119 myokines, 79 adipokines and 22 adipomyokines. Network analysis of these candidates revealed remodelling of extracellular matrix and tissue fibrosis as relevant functions of several of these candidates. Given the pathophysiogical relevance of fibrosis for adipose-muscle-cross-talk in obesity and type 2 diabetes and its physiological role in exercise adaptation and meat quality of farm animals, they represent interesting candidates for further investigations in different research areas and species.
authors.affiliation: a Institute for Muscle Biology and Growth, Leibniz Institute for Farm Animal Biology , Dummerstorf , Germany .
authors.forename: Lisa
authors.initials: L
authors.lastname: Schering
authors.affiliation: b Division of Clinical Chemistry and Pathobiochemistry , Department of Internal Medicine IV, University Hospital Tübingen , Tübingen , Germany .
authors.forename: Miriam
authors.initials: M
authors.lastname: Hoene
authors.affiliation: c Department of Experimental Diabetology , German Institute of Human Nutrition Potsdam-Rehbruecke, Nuthetal, Germany and German Center for Diabetes Research (DZD) , Neuherberg , Germany .|d German Center for Diabetes Research (DZD) , Neuherberg , Germany .
authors.forename: Timo
authors.initials: T
authors.lastname: Kanzleiter
authors.affiliation: c Department of Experimental Diabetology , German Institute of Human Nutrition Potsdam-Rehbruecke, Nuthetal, Germany and German Center for Diabetes Research (DZD) , Neuherberg , Germany .|d German Center for Diabetes Research (DZD) , Neuherberg , Germany .
authors.forename: Markus
authors.initials: M
authors.lastname: Jähnert
authors.affiliation: e Institute for Genome Biology, Leibniz Institute for Farm Animal Biology , Dummerstorf , Germany .
authors.forename: Klaus
authors.initials: K
authors.lastname: Wimmers
authors.affiliation: f Group of Energy Metabolism, German Institute of Human Nutrition Potsdam-Rehbruecke , Nuthetal , Germany , and.
authors.forename: Susanne
authors.initials: S
authors.lastname: Klaus
authors.affiliation: d German Center for Diabetes Research (DZD) , Neuherberg , Germany .|g Paul-Langerhans-Group for Integrative Physiology, German Diabetes Center , Düsseldorf , Germany.
authors.forename: Jürgen
authors.initials: J
authors.lastname: Eckel
authors.affiliation: b Division of Clinical Chemistry and Pathobiochemistry , Department of Internal Medicine IV, University Hospital Tübingen , Tübingen , Germany .|d German Center for Diabetes Research (DZD) , Neuherberg , Germany .
authors.forename: Cora
authors.initials: C
authors.lastname: Weigert
authors.affiliation: c Department of Experimental Diabetology , German Institute of Human Nutrition Potsdam-Rehbruecke, Nuthetal, Germany and German Center for Diabetes Research (DZD) , Neuherberg , Germany .|d German Center for Diabetes Research (DZD) , Neuherberg , Germany .
authors.forename: Annette
authors.initials: A
authors.lastname: Schürmann
authors.affiliation: a Institute for Muscle Biology and Growth, Leibniz Institute for Farm Animal Biology , Dummerstorf , Germany .
authors.forename: Steffen
authors.initials: S
authors.lastname: Maak
authors.affiliation: c Department of Experimental Diabetology , German Institute of Human Nutrition Potsdam-Rehbruecke, Nuthetal, Germany and German Center for Diabetes Research (DZD) , Neuherberg , Germany .|d German Center for Diabetes Research (DZD) , Neuherberg , Germany .
authors.forename: Wenke
authors.initials: W
authors.lastname: Jonas
authors.affiliation: d German Center for Diabetes Research (DZD) , Neuherberg , Germany .|g Paul-Langerhans-Group for Integrative Physiology, German Diabetes Center , Düsseldorf , Germany.
authors.forename: Henrike
authors.initials: H
authors.lastname: Sell
mesh_terms: D054392:Adipokines / Q000235:genetics / Q000378:metabolism*; D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D059305:Diet, High-Fat / Q000009:adverse effects; D004195:Disease Models, Animal; D053263:Gene Regulatory Networks; D006801:Humans; D007473:Ion Channels / Q000502:physiology; D008297:Male; D024821:Metabolic Syndrome / Q000503:physiopathology; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D024101:Mitochondrial Proteins / Q000502:physiology; D018482:Muscle, Skeletal / Q000166:cytology / Q000378:metabolism*; D009765:Obesity / Q000209:etiology / Q000378:metabolism*; D010805:Physical Conditioning, Animal; D020543:Proteome; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013045:Species Specificity; D059467:Transcriptome; D000071256:Uncoupling Protein 1
chemical_list: D054392:Adipokines; D016207:Cytokines; D007473:Ion Channels; D024101:Mitochondrial Proteins; D020543:Proteome; D012333:RNA, Messenger; D000071256:Uncoupling Protein 1
keywords: Adipokine; exercise; fibrosis; myokine; obesity