title: [VISUALIZATION OF THREE-DIMENSIONAL RELIEF STRUCTURES OF SEROUS MEMBRANES WITH A LIGHT MICROSCOPE].
abstract: Methods for three-dimensional visualization of histological film preparations were developed using traditional flat-field microscopes. Preliminarily, the structures examined acquired the light reflecting ability by the reduction of silver nitrate impregnating them. Microscopy of preparations is carried out in reflected incident tenebrous illumination. The methods of processing of preparations for the study of cell surface relief, limiting membrane of the mesothelium and subsurface collagen fibers. Preparations are stored and examined in the wet state.
authors.forename: R S
authors.initials: RS
authors.lastname: Minigazimov
authors.forename: V Sh
authors.initials: VSh
authors.lastname: Vagapova
authors.forename: G R
authors.initials: GR
authors.lastname: Mukhametshina
mesh_terms: D000818:Animals; D003094:Collagen / Q000648:ultrastructure; D006801:Humans; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008853:Microscopy / Q000379:methods; D061848:Optical Imaging / Q000379:methods*; D012704:Serous Membrane / Q000166:cytology*; D012835:Silver Nitrate / Q000737:chemistry; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D003094:Collagen; D012835:Silver Nitrate