title: Differential roles of the hemerythrin-like proteins of Mycobacterium smegmatis in hydrogen peroxide and erythromycin susceptibility.
abstract: Hemerythrin-like proteins are oxygen-carrying non-heme di-iron binding proteins and their functions have effect on oxidation-reduction regulation and antibiotic resistance. Recent studies using bioinformatic analyses suggest that multiple hemerythrin-like protein coding sequences might have been acquired by lateral gene transfer and the number of hemerythrin-like proteins varies amongst different species. Mycobacterium smegmatis contains three hemerythrin-like proteins, MSMEG_3312, MSMEG_2415 and MSMEG_6212. In this study, we have systematically analyzed all three hemerythrin-like proteins in M. smegmatis and our results identified and characterized two functional classes: MSMEG_2415 plays an important role in H2O2 susceptibility, and MSMEG_3312 and MSMEG_6212 are associated with erythromycin susceptibility. Phylogenetic analysis indicated that these three proteins have different evolutionary origins, possibly explaining their different physiological functions. Here, combined with biological and phylogenetic analyses, our results provide new insights into the evolutionary divergence of the hemerythrin-like proteins in M. smegmatis.
authors.affiliation: CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, CAS, Beijing 100101, China.
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Li
authors.affiliation: CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, CAS, Beijing 100101, China.|School of Life Sciences, Anhui University, Hefei 230601, Anhui Province, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Li
authors.affiliation: CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, CAS, Beijing 100101, China.
authors.forename: Xinling
authors.initials: X
authors.lastname: Hu
authors.affiliation: CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, CAS, Beijing 100101, China.
authors.forename: Lige
authors.initials: L
authors.lastname: Huang
authors.affiliation: CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, CAS, Beijing 100101, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Xiao
authors.affiliation: Division of Infectious and Diseases, Department of Medicine, Albert Einstein College of Medicine, New York 10461, USA.
authors.forename: John
authors.initials: J
authors.lastname: Chan
authors.affiliation: CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, CAS, Beijing 100101, China.|Beijing Key Laboratory of Microbial Drug Resistance and Resistome, Beijing 100101, China.
authors.forename: Kaixia
authors.initials: K
authors.lastname: Mi
mesh_terms: D000222:Adaptation, Physiological / Q000187:drug effects; D001426:Bacterial Proteins / Q000378:metabolism*; D004917:Erythromycin / Q000494:pharmacology*; D055786:Gene Knockout Techniques; D006422:Hemerythrin / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D008954:Models, Biological; D020102:Mycobacterium smegmatis / Q000187:drug effects* / Q000378:metabolism*; D010802:Phylogeny
chemical_list: D001426:Bacterial Proteins; D006422:Hemerythrin; D004917:Erythromycin; D006861:Hydrogen Peroxide