title: Downregulation of microRNA-586 Inhibits Proliferation, Invasion and Metastasis and Promotes Apoptosis in Human Osteosarcoma U2-OS Cell Line.
abstract: In this study, we aim to examine the association of microRNA-586 (miR-586) with osteosarcoma (OS) cell proliferation, apoptosis, invasion, and metastasis. U2-OS cell lines were divided into 4 groups: an miR-586 group, anti-miR-586 group, control group (empty plasmid) and blank group (no plasmid). qRT-PCR was used to detect miR-586 expression, cell counting kit-8 and EdU assays to detect cell proliferation, flow cytometry to detect cell cycle distribution, Annexin V/PI double staining to detect cell apoptosis, and the Transwell assay to detect cell invasion and metastasis. miR-586 expression was significantly higher in the miR-586 group but significantly lower in the anti-miR-586 group compared with the control and blank groups. Cell proliferation at 2-5 days after cell transfection and the EdU-positive cell number increased obviously in the miR-586 group but decreased clearly in the anti-miR-586 group. In the miR-586 group, cells at G0/G1 stage and apoptosis cells significantly decreased, while cells at G2/M and S stages and invasive and metastatic cells significantly increased compared to the control and blank groups; however, opposite trends were found in the anti-miR-586 group. Downregulation of miR-586 expression in OS may inhibit cell proliferation, invasion and metastasis, and promote cell apoptosis.
authors.affiliation: Department of Radiology, Jilin Cancer Hospital, Changchun, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Yang
authors.forename: Zong-Ming
authors.initials: ZM
authors.lastname: Liu
authors.forename: Yan-Wei
authors.initials: YW
authors.lastname: Rao
authors.forename: Shao-Qian
authors.initials: SQ
authors.lastname: Cui
authors.forename: Huan
authors.initials: H
authors.lastname: Wang
authors.forename: Xiao-Jing
authors.initials: XJ
authors.lastname: Jia
mesh_terms: D017209:Apoptosis / Q000235:genetics*; D001859:Bone Neoplasms / Q000235:genetics / Q000473:pathology*; D002453:Cell Cycle; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics*; D005434:Flow Cytometry; D005822:Genetic Vectors; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D009361:Neoplasm Invasiveness / Q000235:genetics*; D009362:Neoplasm Metastasis / Q000235:genetics*; D012516:Osteosarcoma / Q000235:genetics / Q000473:pathology*; D014162:Transfection
chemical_list: C000607475:MIRN586 microRNA, human; D035683:MicroRNAs