title: Oxidative stress and spermatogenesis suppression in the testis of cadmium-treated Bombyx mori larvae.
abstract: Bombyx mori L. (B. mori) were exposed to cadmium chloride (CdCl2) incorporated in an artificial diet (0, 6.25, 12.5, 25, and 50 mg kg(-1)) throughout the larval stage. Changes in malondialdehyde (MDA) and reduced glutathione (GSH) contents and activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px), as well as their corresponding messenger RNA (mRNA) levels in the testes of the fifth instar larvae were evaluated. Additionally, spermatozoon deformation in the testes was examined. Upon Cd treatment, the MDA content in the testes was significantly increased in a concentration-dependent manner. Cd-exposed larvae had increased levels of glutathione. Pearson's correlation analysis revealed that SOD and CAT activities were positively correlated (R (2) = 0.605, P = 0.017). The changing trends in the mRNA levels of these enzymes were not always consistent with those of enzymatic activities. Alterations in GSH-Px activities and mRNA levels were positively correlated (R (2) = 0.771, P < 0.01). Morphological analysis revealed that Cd deformed and affected the maturation of spermatozoa. Our results collectively support a relationship between Cd and alterations in the levels of antioxidant enzymes in B. mori testes.
authors.affiliation: School of Chemistry Biology and Material Engineering, Jiangsu Key Laboratory for Environment Functional Materials, Suzhou University of Science and Technology, Suzhou, Jiangsu, 215009, China. yuanhx@mail.usts.edu.cn.|Suzhou National New & Hi-tech Industrial Development Zone, Kerui Road 1, Suzhou, 215009, China. yuanhx@mail.usts.edu.cn.
authors.forename: Hongxia
authors.initials: H
authors.lastname: Yuan
authors.affiliation: School of Chemistry Biology and Material Engineering, Jiangsu Key Laboratory for Environment Functional Materials, Suzhou University of Science and Technology, Suzhou, Jiangsu, 215009, China.
authors.forename: Fenjv
authors.initials: F
authors.lastname: Qin
authors.affiliation: School of Chemistry Biology and Material Engineering, Jiangsu Key Laboratory for Environment Functional Materials, Suzhou University of Science and Technology, Suzhou, Jiangsu, 215009, China.
authors.forename: Weiqiang
authors.initials: W
authors.lastname: Guo
authors.affiliation: School of Chemistry Biology and Material Engineering, Jiangsu Key Laboratory for Environment Functional Materials, Suzhou University of Science and Technology, Suzhou, Jiangsu, 215009, China.
authors.forename: Huajie
authors.initials: H
authors.lastname: Gu
authors.affiliation: School of Chemistry Biology and Material Engineering, Jiangsu Key Laboratory for Environment Functional Materials, Suzhou University of Science and Technology, Suzhou, Jiangsu, 215009, China.
authors.forename: Aihua
authors.initials: A
authors.lastname: Shao
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D012831:Bombyx / Q000187:drug effects*; D019256:Cadmium Chloride / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D007814:Larva / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress; D013091:Spermatogenesis / Q000187:drug effects*; D013482:Superoxide Dismutase / Q000378:metabolism; D013737:Testis
chemical_list: D000975:Antioxidants; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione; D019256:Cadmium Chloride
keywords: Antioxidant enzyme; Bombyx mori; Cadmium; Testes; mRNA expression