title: 1,3-Propanediol production from glycerol with a novel biocatalyst Shimwellia blattae ATCC 33430: Operational conditions and kinetics in batch cultivations.
abstract: Shimwellia blattae ATCC 33430 as biocatalyst in the conversion of 1,3-propanediol from glycerol is herein evaluated. Several operational conditions in batch cultivations, employing pure and raw glycerol as sole carbon source, were studied. Temperature was studied at shaken bottle scale, while pH control strategy, together with the influence of raw glycerol and its impurities during fermentation were studied employing a 2L STBR. Thereafter, fluid dynamic conditions were considered by changing the stirring speed and the gas supply (air or nitrogen) in the same scale-up experiments. The best results were obtained at a temperature of 37°C, an agitation rate of 200rpm, with free pH evolution from 6.9 and subsequent control at 6.5 and no gas supply during the fermentation, employing an initial concentration of 30g/L of raw glycerol. Under these conditions, the biocatalyst is competitive, leading to results in line with other previous works in the literature in batch conditions, reaching a final concentration of 1,3-propanediol of 13.84g/L, with a yield of 0.45g/g and a productivity of 1.19g/(Lh) from raw glycerol.
authors.affiliation: Department of Chemical Engineering, Faculty of Chemistry, University Complutense of Madrid, Spain.
authors.forename: Alberto
authors.initials: A
authors.lastname: Rodriguez
authors.affiliation: Department of Chemical Engineering, Faculty of Chemistry, University Complutense of Madrid, Spain.
authors.forename: Mateusz
authors.initials: M
authors.lastname: Wojtusik
authors.affiliation: Department of Chemical Engineering, Faculty of Chemistry, University Complutense of Madrid, Spain.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Ripoll
authors.affiliation: Department of Chemical Engineering, Faculty of Chemistry, University Complutense of Madrid, Spain. Electronic address: vesantos@ucm.es.
authors.forename: Victoria E
authors.initials: VE
authors.lastname: Santos
authors.affiliation: Department of Chemical Engineering, Faculty of Chemistry, University Complutense of Madrid, Spain.
authors.forename: F
authors.initials: F
authors.lastname: Garcia-Ochoa
mesh_terms: D000332:Aerobiosis; D001419:Bacteria / Q000254:growth & development* / Q000378:metabolism*; D061249:Batch Cell Culture Techniques / Q000379:methods*; D055162:Biocatalysis; D001709:Biotechnology / Q000379:methods*; D005285:Fermentation; D005990:Glycerol / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D011409:Propylene Glycols / Q000378:metabolism*; D013696:Temperature
chemical_list: D011409:Propylene Glycols; C041787:1,3-propanediol; D005990:Glycerol
keywords: 1,3-Propanediol; Batch fermentation; Raw glycerol; Shimwellia blattae CECT 33430