title: Bone marrow mesenchymal stem cell aggregate: an optimal cell therapy for full-layer cutaneous wound vascularization and regeneration.
abstract: Cutaneous wounds are among the most common soft tissue injuries. Wounds involving dermis suffer more from outside influence and higher risk of chronic inflammation. Therefore the appearance and function restoration has become an imperative in tissue engineering research. In this study, cell-aggregates constructed with green fluorescent protein-expressing (GFP(+)) rat bone marrow mesenchymal stem cells (BMMSCs) were applied to rat acute full-layer cutaneous wound model to confirm its pro-regeneration ability and compare its regenerative efficacy with the currently thriving subcutaneous and intravenous stem cell administration strategy, with a view to sensing the advantages, disadvantages and the mechanism behind. According to results, cell-aggregates cultured in vitro enjoyed higher expression of several pro-healing genes than adherent cultured cells. Animal experiments showed better vascularization along with more regular dermal collagen deposition for cell-aggregate transplanted models. Immunofluorescence staining on inflammatory cells indicated a shorter inflammatory phase for cell-aggregate group, which was backed up by further RT-PCR. The in situ immunofluorescence staining manifested a higher GFP(+)-cell engraftment for cell-aggregate transplanted models versus cell administered ones. Thus it is safe to say the BMMSCs aggregate could bring superior cutaneous regeneration for full layer cutaneous wound to BMMSCs administration, both intravenous and subcutaneous.
authors.affiliation: State Key Laboratory of Military Stomatology, Center of Tissue Engineering, School of Stomatology, The Fourth Military Medical University, No. 145 Changlexi Road, Xi'an, Shaanxi 710032, China.|Research and Development Center for Tissue Engineering, Fourth Military Medical University, No. 145 Changlexi Road, Xi'an, Shaanxi 710032, China.|Zhen Jiang Entry-Exit Inspection And Quarantine Bureau, No. 84 Dongwu Road, Zhen Jiang, Jiang Su 212000, China.
authors.forename: Yulin
authors.initials: Y
authors.lastname: An
authors.affiliation: State Key Laboratory of Military Stomatology, Center of Tissue Engineering, School of Stomatology, The Fourth Military Medical University, No. 145 Changlexi Road, Xi'an, Shaanxi 710032, China.|Research and Development Center for Tissue Engineering, Fourth Military Medical University, No. 145 Changlexi Road, Xi'an, Shaanxi 710032, China.|State Key Laboratory of Military Stomatology, Department of Periodontology, School of Stomatology, The Fourth Military Medical University, No. 145 Changlexi Road, Xi'an 710032, China.
authors.forename: Wei
authors.initials: W
authors.lastname: wei
authors.affiliation: State Key Laboratory of Military Stomatology, Center of Tissue Engineering, School of Stomatology, The Fourth Military Medical University, No. 145 Changlexi Road, Xi'an, Shaanxi 710032, China.|Research and Development Center for Tissue Engineering, Fourth Military Medical University, No. 145 Changlexi Road, Xi'an, Shaanxi 710032, China.
authors.forename: Huan
authors.initials: H
authors.lastname: Jing
authors.affiliation: State Key Laboratory of Military Stomatology, Center of Tissue Engineering, School of Stomatology, The Fourth Military Medical University, No. 145 Changlexi Road, Xi'an, Shaanxi 710032, China.|Research and Development Center for Tissue Engineering, Fourth Military Medical University, No. 145 Changlexi Road, Xi'an, Shaanxi 710032, China.
authors.forename: Leiguo
authors.initials: L
authors.lastname: Ming
authors.affiliation: State Key Laboratory of Military Stomatology, Center of Tissue Engineering, School of Stomatology, The Fourth Military Medical University, No. 145 Changlexi Road, Xi'an, Shaanxi 710032, China.|Research and Development Center for Tissue Engineering, Fourth Military Medical University, No. 145 Changlexi Road, Xi'an, Shaanxi 710032, China.
authors.forename: Shiyu
authors.initials: S
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Military Stomatology, Center of Tissue Engineering, School of Stomatology, The Fourth Military Medical University, No. 145 Changlexi Road, Xi'an, Shaanxi 710032, China.|Research and Development Center for Tissue Engineering, Fourth Military Medical University, No. 145 Changlexi Road, Xi'an, Shaanxi 710032, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Jin
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000502:physiology; D002449:Cell Aggregation; D064987:Cell- and Tissue-Based Therapy / Q000379:methods*; D024042:Collagen Type I / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology; D018919:Neovascularization, Physiologic; D051381:Rats; D017207:Rats, Sprague-Dawley; D055647:Rats, Transgenic; D012867:Skin / Q000293:injuries; D016212:Transforming Growth Factor beta / Q000235:genetics / Q000378:metabolism; D014945:Wound Healing / Q000502:physiology*; D014949:Wounds, Nonpenetrating / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000628:therapy*
chemical_list: D024042:Collagen Type I; D016212:Transforming Growth Factor beta; D049452:Green Fluorescent Proteins