title: Identification and Biological Characterization of Leishmania (Viannia) guyanensis Isolated from a Patient with Tegumentary Leishmaniasis in Goiás, a Nonendemic Area for This Species in Brazil.
abstract: The aim of this study was to characterize clinical field isolates of Leishmania spp. obtained from patients with American Tegumentary Leishmaniasis (ATL) who live in Goiás state, Brazil. The presumed areas of infection were in Goiás, Tocantins, and Pará states. Three isolates of parasites were identified as L. (Viannia) braziliensis and one as L. (V.) guyanensis. The in vitro growth profiles were found to be similar for all parasites. Nevertheless, in C57BL/6 mice, L. (V.) guyanensis infection was better controlled than L. (V.) braziliensis. Yet in C57BL/6 mice deficient in interferon gamma, L. (V.) guyanensis lesions developed faster than those caused by L. (V.) braziliensis isolates. In BALB/c mice, the development of lesions was similar for isolates from both species; however, on the 11th week of infection, amastigotes could not be observed in macrophages from L. (V.) guyanensis-infected mice. Thus, L. (V.) guyanensis can be circulating in Goiás, a state where autochthonous cases of this species had not yet been reported. Considering the difficulties to differentiate L. (V.) guyanensis from L. (V.) braziliensis at the molecular, morphological, and clinical (human and murine models) levels, the presence of L. (V.) guyanensis infections is possibly underestimated in several regions of Brazil.
authors.affiliation: Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Rua 235 S/N, Setor Universitário, 74605-050 Goiânia, GO, Brazil.
authors.forename: Alause da Silva
authors.initials: Ada S
authors.lastname: Pires
authors.affiliation: Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Rua 235 S/N, Setor Universitário, 74605-050 Goiânia, GO, Brazil.
authors.forename: Arissa Felipe
authors.initials: AF
authors.lastname: Borges
authors.affiliation: Instituto de Ciências Biomédicas, Universidade de São Paulo, SP, Brazil.
authors.forename: Adriano
authors.initials: A
authors.lastname: Cappellazzo Coelho
authors.affiliation: Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Rua 235 S/N, Setor Universitário, 74605-050 Goiânia, GO, Brazil.
authors.forename: Miriam Leandro
authors.initials: ML
authors.lastname: Dorta
authors.affiliation: Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Rua 235 S/N, Setor Universitário, 74605-050 Goiânia, GO, Brazil.
authors.forename: Ruy de Souza
authors.initials: Rde S
authors.lastname: Lino Junior
authors.affiliation: Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Rua 235 S/N, Setor Universitário, 74605-050 Goiânia, GO, Brazil.
authors.forename: Ledice Inacia de Araújo
authors.initials: LI
authors.lastname: Pereira
authors.affiliation: Instituto Goiano de Oncologia e Hematologia e Faculdade de Medicina, Universidade Federal de Goiás, GO, Brazil.
authors.forename: Sebastião Alves
authors.initials: SA
authors.lastname: Pinto
authors.affiliation: Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Rua 235 S/N, Setor Universitário, 74605-050 Goiânia, GO, Brazil.
authors.forename: Milton Adriano
authors.initials: MA
authors.lastname: Pelli de Oliveira
authors.affiliation: Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Rua 235 S/N, Setor Universitário, 74605-050 Goiânia, GO, Brazil.
authors.forename: Grazzielle Guimarães
authors.initials: GG
authors.lastname: de Matos
authors.affiliation: Universidade de São Paulo, SP, Brazil.
authors.forename: Ises A
authors.initials: IA
authors.lastname: Abrahamsohn
authors.affiliation: Instituto de Ciências Biomédicas, Universidade de São Paulo, SP, Brazil.
authors.forename: Silvia Reni B
authors.initials: SR
authors.lastname: Uliana
authors.affiliation: Universidade de São Paulo, SP, Brazil.
authors.forename: Glória Maria Collet de Araújo
authors.initials: GM
authors.lastname: Lima
authors.affiliation: Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Rua 235 S/N, Setor Universitário, 74605-050 Goiânia, GO, Brazil.
authors.forename: Fátima
authors.initials: F
authors.lastname: Ribeiro-Dias
mesh_terms: D000818:Animals; D001938:Brazil; D006801:Humans; D007892:Leishmania braziliensis / Q000302:isolation & purification / Q000472:pathogenicity*; D018334:Leishmania guyanensis / Q000302:isolation & purification / Q000472:pathogenicity*; D016773:Leishmaniasis, Cutaneous / Q000469:parasitology* / Q000473:pathology; D051379:Mice