title: [Expression of microRNA-100 and its correlation with drug resistance in human ovarian cancer SKOV3/DDP cells].
abstract: OBJECTIVE
  To investigate the expression of microRNA-100(miR-100) and the relationship with cisplatin resistance in human ovarian epithelial cancer SKOV3/DDP cells.
  
  
  METHODS
  The SKOV3/DDP cells were transfected with the mimics or inhibitor of miR-100 or negative control RNA (NC) or inhibitor negative control RNA (inhibitor NC) by lipofectamine 2000. The experiment was divided into six groups: SKOV3 group, SKOV3/DDP group, miR-100 mimices group, NC group, miR-100 inhibitor group and inhibitor NC group. The expression of miR-100 and the cisplatin IC50 were measured by real-time PCR and CCK8 assay respectively.
  
  
  RESULTS
  (1)The cisplatin resistance index of SKOV3/DDP was 2.23; (2)The express level of miR-100 in SKOV3/DDP cells was significantly lower than that in SKOV3 cells (P<0.001); (3)After transfected with miR-100 mimics, SKOV3/DDP cells showed that the level of miR-100 was 38.29 times higher than that in the NC group(P<0.01). The cisplatin IC50 of miR-100 mimices group was significantly lower than that in the NC group (P<0.001); (4) After transfected with miR-100 inhibitor, the level of miR-100 0f SKOV3/DDP was decreased by 97.7%. The cisplatin IC50 of miR-100 inhibitor group was significantly increased as compared with that in the inhibitor NC group (P<0.001).
  
  
  CONCLUSIONS
  The expression of miR-100 is downregulated in SKOV3/DDP cells. Overexpressing miR-100 may effectively increase the sensitivity to cisplatin of human ovarian epithelial cancer SKOV3/DDP cells and may reverse cisplatin-resistance of EOC (epithelial ovarian cancer).
authors.affiliation: Department of Obstetrics & Gynecology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.E-mail: guop0819@163.com.
authors.forename: Peng
authors.initials: P
authors.lastname: Guo
authors.forename: Dongxian
authors.initials: D
authors.lastname: Peng
authors.forename: Xiangpeng
authors.initials: X
authors.lastname: Xiong
authors.forename: Sainan
authors.initials: S
authors.lastname: Zhang
mesh_terms: D000970:Antineoplastic Agents; D000077216:Carcinoma, Ovarian Epithelial; D045744:Cell Line, Tumor; D002945:Cisplatin / Q000494:pharmacology*; D015536:Down-Regulation; D019008:Drug Resistance, Neoplasm; D005260:Female; D006801:Humans; D035683:MicroRNAs / Q000378:metabolism*; D009375:Neoplasms, Glandular and Epithelial / Q000378:metabolism* / Q000473:pathology; D010051:Ovarian Neoplasms / Q000378:metabolism* / Q000473:pathology; D060888:Real-Time Polymerase Chain Reaction; D014162:Transfection
chemical_list: D000970:Antineoplastic Agents; C544266:MIRN100 microRNA, human; D035683:MicroRNAs; D002945:Cisplatin