title: Enhanced production of 3-hydroxypropionic acid from glucose via malonyl-CoA pathway by engineered Escherichia coli.
abstract: In this study, production of 3-HP via malonyl-CoA was investigated by using metabolically engineered Escherichia coli carrying heterogeneous acetyl-CoA carboxylase (Acc) from Corynebacterium glutamicum and codon-optimized malonyl-CoA reductase (MCR) from Chloroflexus aurantiacus. Three engineered E. coli strains with different host-vector systems were constructed and investigated. The results indicated that the combination of E. coli BL21(DE3) and pET28a was the most efficient host-vector system for 3-HP production, and the highest concentration of 3-HP attained in shake flask cultivation reached 1.80g/L by the strain BE-MDA with induction at 0.25mM IPTG and 25°C, and supplementation of NaHCO3 and biotin. In fed-batch fermentation performed in a 5-L reactor, the concentration of 3-HP achieved 10.08g/L in 36h.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China.
authors.forename: Zhuan
authors.initials: Z
authors.lastname: Cheng
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China.
authors.forename: Jiaqi
authors.initials: J
authors.lastname: Jiang
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China; Shanghai Collaborative Innovation Center for Biomanufacturing Technology, 130 Meilong Road, Shanghai 200237, China. Electronic address: lizm@ecust.edu.cn.
authors.forename: Zhimin
authors.initials: Z
authors.lastname: Li
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Ye
mesh_terms: D000085:Acetates / Q000378:metabolism; D000103:Acetyl-CoA Carboxylase / Q000378:metabolism; D061249:Batch Cell Culture Techniques; D019149:Bioreactors / Q000382:microbiology; D001710:Biotin / Q000494:pharmacology; D048230:Corynebacterium glutamicum / Q000187:drug effects / Q000378:metabolism; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D005285:Fermentation / Q000187:drug effects; D005947:Glucose / Q000378:metabolism*; D007544:Isopropyl Thiogalactoside / Q000494:pharmacology; D019344:Lactic Acid / Q000031:analogs & derivatives* / Q000096:biosynthesis; D008316:Malonyl Coenzyme A / Q000378:metabolism*; D060847:Metabolic Engineering / Q000379:methods*; D053858:Metabolic Networks and Pathways / Q000187:drug effects; D010088:Oxidoreductases / Q000378:metabolism; D017693:Sodium Bicarbonate / Q000494:pharmacology; D013997:Time Factors
chemical_list: D000085:Acetates; D019344:Lactic Acid; D007544:Isopropyl Thiogalactoside; D008316:Malonyl Coenzyme A; D001710:Biotin; D017693:Sodium Bicarbonate; C031601:hydracrylic acid; D010088:Oxidoreductases; C454539:malonyl-Coa reductase; D000103:Acetyl-CoA Carboxylase; D005947:Glucose
keywords: 3-Hydroxypropionate; Acetyl-CoA carboxylase; Corynebacterium glutamicum; Escherichia coli; Malonyl-CoA reductase