title: Acetylation of an NB-LRR Plant Immune-Effector Complex Suppresses Immunity.
abstract: Modifications of plant immune complexes by secreted pathogen effectors can trigger strong immune responses mediated by the action of nucleotide binding-leucine-rich repeat immune receptors. Although some strains of the pathogen Pseudomonas syringae harbor effectors that individually can trigger immunity, the plant's response may be suppressed by other virulence factors. This work reveals a robust strategy for immune suppression mediated by HopZ3, an effector in the YopJ family of acetyltransferases. The suppressing HopZ3 effector binds to and can acetylate multiple members of the RPM1 immune complex, as well as two P. syringae effectors that together activate the RPM1 complex. These acetylations modify serine, threonine, lysine, and/or histidine residues in the targets. Through HopZ3-mediated acetylation, it is possible that the whole effector-immune complex is inactivated, leading to increased growth of the pathogen.
authors.affiliation: Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, IL 60637, USA.
authors.forename: Jiyoung
authors.initials: J
authors.lastname: Lee
authors.affiliation: Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, IL 60637, USA.
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Manning
authors.affiliation: Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, IL 60637, USA.
authors.forename: Donald
authors.initials: D
authors.lastname: Wolfgeher
authors.affiliation: Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, IL 60637, USA.
authors.forename: Joanna
authors.initials: J
authors.lastname: Jelenska
authors.affiliation: The Genome Center & Department of Plant Sciences, University of California, Davis, Davis, CA 95616, USA.
authors.forename: Keri A
authors.initials: KA
authors.lastname: Cavanaugh
authors.affiliation: The Genome Center & Department of Plant Sciences, University of California, Davis, Davis, CA 95616, USA.
authors.forename: Huaqin
authors.initials: H
authors.lastname: Xu
authors.affiliation: Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, IL 60637, USA.
authors.forename: Sandra M
authors.initials: SM
authors.lastname: Fernandez
authors.affiliation: The Genome Center & Department of Plant Sciences, University of California, Davis, Davis, CA 95616, USA.
authors.forename: Richard W
authors.initials: RW
authors.lastname: Michelmore
authors.affiliation: Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, IL 60637, USA.
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Kron
authors.affiliation: Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, IL 60637, USA. Electronic address: jgreenbe@uchicago.edu.
authors.forename: Jean T
authors.initials: JT
authors.lastname: Greenberg
mesh_terms: D000107:Acetylation; D000123:Acetyltransferases / Q000276:immunology / Q000378:metabolism; D000596:Amino Acids / Q000276:immunology / Q000378:metabolism; D000936:Antigen-Antibody Complex / Q000276:immunology* / Q000378:metabolism*; D010935:Plant Diseases / Q000276:immunology / Q000382:microbiology; D057865:Plant Immunity / Q000276:immunology*; D010940:Plant Proteins / Q000276:immunology* / Q000378:metabolism*; D044224:Pseudomonas syringae / Q000276:immunology; D037521:Virulence Factors / Q000276:immunology
chemical_list: D000596:Amino Acids; D000936:Antigen-Antibody Complex; D010940:Plant Proteins; D037521:Virulence Factors; D000123:Acetyltransferases