title: "Dilute-and-shoot" rapid-separation liquid chromatography tandem mass spectrometry method for fast detection of thiodiglycolic acid in urine.
abstract: A new sensitive rapid-separation liquid chromatography tandem mass spectrometry approach for the determination of thiodiglycolic acid (TDGA) in urine has been developed. The use of the "dilute-and-shoot" method helps to shorten the sample preparation stage and provides a sensitive and direct approach for TDGA determination in urine. Chromatographic separation of the analyte and other urine compounds was achieved using a reverse-phase liquid chromatography column with mobile phases consisting of 0.1% formic acid in water and acetonitrile in a gradient elution mode. For the identification and quantification of TDGA electrospray ionization-tandem mass spectrometry monitoring, two precursor-to-product ion transitions were used. The method demonstrates good linearity and has a detection limit of 50 ng mL⁻¹ in urine.
authors.affiliation: Chemistry Department, Lomonosov Moscow State University, 119991 Moscow, Russia. timurbaychem@gmail.com.
authors.forename: Timur
authors.initials: T
authors.lastname: Baygildiev
authors.affiliation: Chemistry Department, Lomonosov Moscow State University, 119991 Moscow, Russia. Kostroma State Technological University, 156005, Kostroma, Russia. avbraun@yandex.ru.
authors.forename: Arcady
authors.initials: A
authors.lastname: Braun
authors.affiliation: Chemistry Department, Lomonosov Moscow State University, 119991 Moscow, Russia. Kostroma State Technological University, 156005, Kostroma, Russia. stavrianidi.andrey@gmail.com.
authors.forename: Andrey
authors.initials: A
authors.lastname: Stavrianidi
authors.affiliation: Chemistry Department, Lomonosov Moscow State University, 119991 Moscow, Russia. igorrodin@yandex.ru.
authors.forename: Igor
authors.initials: I
authors.lastname: Rodin
authors.affiliation: Chemistry Department, Lomonosov Moscow State University, 119991 Moscow, Russia. shpiguno@yandex.ru.
authors.forename: Oleg
authors.initials: O
authors.lastname: Shpigun
authors.affiliation: Kostroma State Technological University, 156005, Kostroma, Russia. rivrus@mail.ru.
authors.forename: Igor
authors.initials: I
authors.lastname: Rybalchenko
authors.affiliation: Chemistry Department, Lomonosov Moscow State University, 119991 Moscow, Russia. irishan@mail.ru.
authors.forename: Irina
authors.initials: I
authors.lastname: Ananieva
mesh_terms: D056148:Chromatography, Reverse-Phase / Q000379:methods*; D006801:Humans; D057230:Limit of Detection; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013864:Thioglycolates / Q000652:urine*
chemical_list: D013864:Thioglycolates; C010518:thiodiacetic acid