title: Single Site Discrimination of Cytosine, 5-Methylcytosine, and 5-Hydroxymethylcytosine in Target DNA Using Anthracene-Tagged Fluorescent Probes.
abstract: The ability to discriminate between epigenetic variants in DNA is a necessary tool if we are to increase our understanding of the roles that they play in various biological processes and medical conditions. Herein, it is demonstrated how a simple two-step fluorescent probe assay can be used to differentiate all three major epigenetic variants of cytosine at a single locus site in a target strand of DNA.
authors.affiliation: School of Chemistry, University of Birmingham , Edgbaston, Birmingham, B15 2TT, United Kingdom.
authors.forename: Jean-Louis H A
authors.initials: JL
authors.lastname: Duprey
authors.affiliation: School of Chemistry, University of Birmingham , Edgbaston, Birmingham, B15 2TT, United Kingdom.
authors.forename: Gemma A
authors.initials: GA
authors.lastname: Bullen
authors.affiliation: School of Chemistry, University of Birmingham , Edgbaston, Birmingham, B15 2TT, United Kingdom.
authors.forename: Zheng-yun
authors.initials: ZY
authors.lastname: Zhao
authors.affiliation: Univ. Bordeaux , CNRS, ISM UMR 5255 351, Cours de la Libération, 33400 Talence, France.
authors.forename: Dario M
authors.initials: DM
authors.lastname: Bassani
authors.affiliation: School of Chemistry, University of Birmingham , Edgbaston, Birmingham, B15 2TT, United Kingdom.
authors.forename: Anna F A
authors.initials: AF
authors.lastname: Peacock
authors.affiliation: School of Chemistry, University of Birmingham , Edgbaston, Birmingham, B15 2TT, United Kingdom.
authors.forename: John
authors.initials: J
authors.lastname: Wilkie
authors.affiliation: School of Chemistry, University of Birmingham , Edgbaston, Birmingham, B15 2TT, United Kingdom.
authors.forename: James H R
authors.initials: JH
authors.lastname: Tucker
mesh_terms: D044503:5-Methylcytosine / Q000737:chemistry* / Q000031:analogs & derivatives; D000873:Anthracenes / Q000737:chemistry*; D003596:Cytosine / Q000031:analogs & derivatives* / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D009690:Nucleic Acid Conformation
chemical_list: D000873:Anthracenes; D005456:Fluorescent Dyes; C011865:5-hydroxymethylcytosine; D044503:5-Methylcytosine; D003596:Cytosine; D004247:DNA; C034020:anthracene