title: Varying hemin concentrations affect Porphyromonas gingivalis strains differently.
abstract: Porphyromonas gingivalis requires heme to grow, however, heme availability and concentration in the periodontal pockets vary. Fluctuations in heme concentration may affect each P. gingivalis strain differently, however, this was never fully demonstrated. Here, we elucidated the effects of varying hemin concentrations in representative P. gingivalis strains. Throughout this study, representative P. gingivalis strains [FDC381 (type I), MPWIb-01 (type Ib), TDC60 (type II), ATCC49417 (type III), W83 (type IV), and HNA99 (type V)] were used and grown for 24 h in growth media under varying hemin concentrations (5 × , 1 × , 0.5 × , 0.1 × ). Samples were lysed and protein standardized. Arg-gingipain (Rgp), H2O2, and superoxide dismutase (SOD) levels were subsequently measured. We focused our study on 24 h-grown strains which excluded MPWIb-01 and HNA99. Rgp activity among the 4 remaining strains varied with Rgp peaking at: 1 × for FDC381, 5 × for TDC60, 0.5 × for ATCC49417, 5 × and 0.5 × for W83. With regards to H2O2 and SOD amounts: FDC381 had similar H2O2 amounts in all hemin concentrations while SOD levels varied; TDC60 had the lowest H2O2 amount at 1 × while SOD levels became higher in relation to hemin concentration; ATCC49417 also had similar H2O2 amounts in all hemin concentrations while SOD levels were higher at 1 × and 0.5 × ; and W83 had statistically similar H2O2 and SOD amounts regardless of hemin concentration. Our results show that variations in hemin concentration affect each P. gingivalis strain differently.
authors.affiliation: Division of Oral Health Science, Nihon University Graduate School of Dentistry, Tokyo 101-8310 Japan; Department of Microbiology, Nihon University School of Dentistry, Tokyo 101-8310 Japan.
authors.forename: Manabu
authors.initials: M
authors.lastname: Ohya
authors.affiliation: Department of Microbiology, Nihon University School of Dentistry, Tokyo 101-8310 Japan. Electronic address: marni.cueno@nihon-u.ac.jp.
authors.forename: Marni E
authors.initials: ME
authors.lastname: Cueno
authors.affiliation: Department of Microbiology, Nihon University School of Dentistry, Tokyo 101-8310 Japan; Division of Immunology and Pathobiology, Dental Research Center, Nihon University School of Dentistry, Tokyo 101-8310 Japan.
authors.forename: Muneaki
authors.initials: M
authors.lastname: Tamura
authors.affiliation: Department of Microbiology, Nihon University School of Dentistry, Tokyo 101-8310 Japan; Division of Immunology and Pathobiology, Dental Research Center, Nihon University School of Dentistry, Tokyo 101-8310 Japan. Electronic address: ochiai.kuniyasu@nihon-u.ac.jp.
authors.forename: Kuniyasu
authors.initials: K
authors.lastname: Ochiai
mesh_terms: D018829:Adhesins, Bacterial / Q000378:metabolism; D003470:Culture Media; D003546:Cysteine Endopeptidases / Q000378:metabolism; D036781:Fimbriae Proteins / Q000235:genetics / Q000378:metabolism; D005838:Genotype; D000080867:Gingipain Cysteine Endopeptidases; D006427:Hemin / Q000008:administration & dosage*; D006861:Hydrogen Peroxide / Q000378:metabolism; D010510:Periodontal Diseases / Q000382:microbiology; D010514:Periodontal Pocket / Q000378:metabolism / Q000382:microbiology; D016966:Porphyromonas gingivalis / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D013045:Species Specificity; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D018829:Adhesins, Bacterial; D003470:Culture Media; D000080867:Gingipain Cysteine Endopeptidases; C063055:fimbrillin; D036781:Fimbriae Proteins; D006427:Hemin; D006861:Hydrogen Peroxide; D013482:Superoxide Dismutase; D003546:Cysteine Endopeptidases
keywords: FimA genotype; Hemin; Periodontal disease infection; Porphyromonas gingivalis