title: Lipidomics of tobacco leaf and cigarette smoke.
abstract: Detailed lipidomics experiments were performed on the extracts of cured tobacco leaf and of cigarette smoke condensate (CSC) using high-resolution liquid chromatography coupled to quadrupole time-of-flight mass spectrometry (LC-Q-TOF MS). Following automated solid-phase extraction (SPE) fractionation of the lipid extracts, over 350 lipids could be annotated. From a large-scale study on 22 different leaf samples, it was determined that differentiation based on curing type was possible for both the tobacco leaf and the CSC extracts. Lipids responsible for the classification were identified and the findings were correlated to proteomics data acquired from the same tobacco leaf samples. Prediction models were constructed based on the lipid profiles observed in the 22 leaf samples and successfully allowed for curing type classification of new tobacco leaves. A comparison of the leaf and CSC data provided insight into the lipidome changes that occur during the smoking process. It was determined that lipids which survive the smoking process retain the same curing type trends in both the tobacco leaf and CSC data.
authors.affiliation: Research Institute for Chromatography (RIC), President Kennedypark 26, 8500 Kortrijk, Belgium.
authors.forename: Melissa N
authors.initials: MN
authors.lastname: Dunkle
authors.affiliation: Japan Tobacco Inc., 6-2 Umegaoka Aoba-ku Yokohama-shi, Kanagawa 227-8512, Japan.
authors.forename: Yuta
authors.initials: Y
authors.lastname: Yoshimura
authors.affiliation: Research Institute for Chromatography (RIC), President Kennedypark 26, 8500 Kortrijk, Belgium.
authors.forename: Ruben
authors.initials: R
authors.lastname: T Kindt
authors.affiliation: Research Institute for Chromatography (RIC), President Kennedypark 26, 8500 Kortrijk, Belgium.
authors.forename: Alexia
authors.initials: A
authors.lastname: Ortiz
authors.affiliation: Japan Tobacco Inc., 6-2 Umegaoka Aoba-ku Yokohama-shi, Kanagawa 227-8512, Japan.
authors.forename: Eri
authors.initials: E
authors.lastname: Masugi
authors.affiliation: Japan Tobacco Inc., 6-2 Umegaoka Aoba-ku Yokohama-shi, Kanagawa 227-8512, Japan.
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Mitsui
authors.affiliation: Research Institute for Chromatography (RIC), President Kennedypark 26, 8500 Kortrijk, Belgium.
authors.forename: Frank
authors.initials: F
authors.lastname: David
authors.affiliation: Research Institute for Chromatography (RIC), President Kennedypark 26, 8500 Kortrijk, Belgium.
authors.forename: Pat
authors.initials: P
authors.lastname: Sandra
authors.affiliation: Research Institute for Chromatography (RIC), President Kennedypark 26, 8500 Kortrijk, Belgium. Electronic address: koen.sandra@richrom.com.
authors.forename: Koen
authors.initials: K
authors.lastname: Sandra
mesh_terms: D002853:Chromatography, Liquid; D008055:Lipids / Q000032:analysis*; D013058:Mass Spectrometry; D018515:Plant Leaves / Q000737:chemistry; D020543:Proteome / Q000032:analysis; D012906:Smoke / Q000032:analysis*; D012907:Smoking; D052616:Solid Phase Extraction; D062789:Tobacco Products
chemical_list: D008055:Lipids; D020543:Proteome; D012906:Smoke
keywords: Lipidomics; Liquid chromatography; Mass spectrometry; Proteomics; Tobacco leaf/smoke