title: [Chemical constituents from stems of Hedyotis hedyotidea and their immunosuppressive activity].
abstract: Hedyotis hedyotidea has been traditionally used for the treatment of arthritis, cold, cough, gastro-enteritis, headstroke, etc. But few studies have screened the active compounds from extracts of H. hedyotidea. In this study, the structure of the chemical constituents from stems of H. hedyotidea were determined and the immunosuppressive activity of the compounds was evaluated. The compounds were separated and purified with silica gel, gel column chromatographies and preparative HPLC, and their structures were identified by spectral methods such as MS and NMR. Eleven compounds were obtained and identified as(6S,9S) -vomifoliol (1), betulonic acid (2), betulinic acid (3), betulin(4), 3-epi-betulinic acid (5), ursolic acid (6), β-sitosterol (7), stigmast-4-en-3-one (8), 7β-hydroxysitosterol (9), (3β,7β) -7-methoxystigmast-5-en-3-ol (10) and morindacin (11). This is the first report of compounds 1, 2, 4, 8, 9, 10 and 11 from H. hedyotidea. Compounds 1, 2 and 8-11 were firstly isolated from the genus Hedyotis, and compounds 9 and 10 were isolated from the family Rubiaceae for the first time. The immunosuppressive activity of these compounds was tested using the lymphocyte transsormationtest. Compounds 4, 6 and 9 showed significant immunosuppressive activity.
authors.forename: Tian-tian
authors.initials: TT
authors.lastname: Zhang
authors.forename: Sha-sha
authors.initials: SS
authors.lastname: Gao
authors.forename: Jun-jie
authors.initials: JJ
authors.lastname: Hou
authors.forename: Yong-qin
authors.initials: YQ
authors.lastname: Zhou
authors.forename: Jie-wen
authors.initials: JW
authors.lastname: Zhou
authors.forename: Xiao-gang
authors.initials: XG
authors.lastname: Wang
authors.forename: Nan
authors.initials: N
authors.lastname: Qin
authors.forename: Jia-chun
authors.initials: JC
authors.lastname: Chen
authors.forename: Hong-quan
authors.initials: HQ
authors.lastname: Duan
authors.forename: Jin-bo
authors.initials: JB
authors.lastname: Fang
mesh_terms: D000818:Animals; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D032064:Hedyotis / Q000737:chemistry*; D007166:Immunosuppressive Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology; D008297:Male; D013058:Mass Spectrometry; D051379:Mice; D008810:Mice, Inbred C57BL; D015394:Molecular Structure; D018547:Plant Stems / Q000737:chemistry*
chemical_list: D004365:Drugs, Chinese Herbal; D007166:Immunosuppressive Agents