title: Enhanced biocatalytic activity of immobilized Pseudomonas cepacia lipase under sonicated condition.
abstract: The present work reports the use of biocatalyst and ultrasound for greener synthesis of cinnamyl propionate. The lipase Pseudomonas cepacia was immobilized on a copolymer of hydroxypropyl methyl cellulose and polyvinyl alcohol. This biocatalyst was u sed for ultrasound-assisted synthesis of cinnamyl propionate with the detailed optimization of various reaction parameters. Besides this, protocol was extended to synthesize various industrially important propionate esters. In addition to this, different enzyme-kinetic parameters such as r max and K m(vinyl propionate), K m(cinnamyl alcohol) and K i(cinnamyl alcohol) were studied which presented ordered bi-bi mechanism with an inhibition by cinnamyl alcohol. The developed biocatalyst demonstrated enhancement in catalytic activity and recyclability up to five recycles. Moreover, the biocatalyst was tested to investigate the effects of sonication via various characterization techniques such as scanning electron microscopy, thermogravimetry, and water content analysis.
authors.affiliation: Department of Chemistry, Institute of Chemical Technology, Matunga, Mumbai, 400 019, India.
authors.forename: Kirtikumar C
authors.initials: KC
authors.lastname: Badgujar
authors.affiliation: Department of Chemistry, Institute of Chemical Technology, Matunga, Mumbai, 400 019, India.
authors.forename: Poorna A
authors.initials: PA
authors.lastname: Pai
authors.affiliation: Department of Chemistry, Institute of Chemical Technology, Matunga, Mumbai, 400 019, India. bm.bhanage@gmail.com.
authors.forename: Bhalchandra M
authors.initials: BM
authors.lastname: Bhanage
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry*; D016956:Burkholderia cepacia / Q000201:enzymology*; D002384:Catalysis; D008049:Lipase / Q000737:chemistry*; D000069453:Ultrasonic Waves
chemical_list: D001426:Bacterial Proteins; D008049:Lipase
keywords: Enhanced activity; Immobilization; Lipase; Sonicated condition