title: Use of an Enrichment Broth Improves Detection of Extended-Spectrum-Beta-Lactamase-Producing Enterobacteriaceae in Clinical Stool Samples.
abstract: This study evaluated the impact of preenrichment on the detection of extended-spectrum beta-lactamase-producing Enterobacteriaceae (ESBL-E) in clinical stool samples. ESBL-E were detected in 41 of 343 patients (12.0%). As 31.7% of the ESBL-E carriers were identified by preenrichment, only this additional diagnostic step significantly improved the detection of ESBL-E.
authors.affiliation: Institute for Medical Microbiology, Immunology and Hygiene, University of Cologne, Cologne, Germany German Centre for Infection Research (DZIF), Partner Site Bonn-Cologne, Cologne, Germany nathalie.jazmati@uk-koeln.de.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Jazmati
authors.affiliation: Institute of Medical Statistics, Informatics and Epidemiology, University of Cologne, Cologne, Germany.
authors.forename: Rebecca
authors.initials: R
authors.lastname: Hein
authors.affiliation: Institute for Medical Microbiology, Immunology and Hygiene, University of Cologne, Cologne, Germany German Centre for Infection Research (DZIF), Partner Site Bonn-Cologne, Cologne, Germany.
authors.forename: Axel
authors.initials: A
authors.lastname: Hamprecht
mesh_terms: D058491:Bacterial Load; D003470:Culture Media; D004755:Enterobacteriaceae / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification*; D004756:Enterobacteriaceae Infections / Q000175:diagnosis* / Q000382:microbiology*; D005243:Feces / Q000382:microbiology*; D006801:Humans; D008826:Microbial Sensitivity Tests; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D001618:beta-Lactamases / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D003470:Culture Media; D001618:beta-Lactamases