title: Influence of age on rat bone-marrow mesenchymal stem cells potential.
abstract: Mesenchymal stem cells promising role in cell-based therapies and tissue engineering appears to be limited due to a decline of their regenerative potential with increasing donor age. Six age groups from bone marrow mesenchymal stem cells of Wistar rats were studied (newborn, infant, young, pre-pubertal, pubertal and adult). Quantitative proteomic assay was performance by iTRAQ using an 8-plex iTRAQ labeling and the proteins differentially expressed were grouped in pluripotency, proliferative and metabolism processes. Proliferation makers, CD117 and Ki67 were measure by flow cytometry assay. Real time polymerase chain reaction analysis of pluripotency markers Rex1, Oct4, Sox2 and Nanog were done. Biological differentiation was realized using specific mediums for 14 days to induce osteogenesis, adipogenesis or chondrogenesis and immunostain analysis of differentiated cell resulting were done. Enzimoimmunoassay analysis of several enzymes as L-lactate dehydrogenase and glucose-6-phosphate isomerase were also done to validate iTRAQ data. Taking together these results indicate for the first time that mesenchymal stem cells have significant differences in their proliferative, pluripotency and metabolism profiles and those differences are age depending.
authors.affiliation: Grupo de Terapia Celular y Medicina Regenerativa (TCMR-CHUAC). CIBER-BBN/ISCIII. Servicio de Reumatología. Instituto de Investigación Biomédica de A Coruña (INIBIC). Complexo Hospitalario Universitario de A Coruña (CHUAC). SERGAS. Departamento de Medicina. Facultade de Oza. Universidade de A Coruña (UDC). As Xubias, 15006. A Coruña, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Fafián-Labora
authors.affiliation: Grupo de Terapia Celular y Medicina Regenerativa (TCMR-CHUAC). CIBER-BBN/ISCIII. Servicio de Reumatología. Instituto de Investigación Biomédica de A Coruña (INIBIC). Complexo Hospitalario Universitario de A Coruña (CHUAC). SERGAS. Departamento de Medicina. Facultade de Oza. Universidade de A Coruña (UDC). As Xubias, 15006. A Coruña, Spain.
authors.forename: P
authors.initials: P
authors.lastname: Fernández-Pernas
authors.affiliation: Grupo de Terapia Celular y Medicina Regenerativa (TCMR-CHUAC). CIBER-BBN/ISCIII. Servicio de Reumatología. Instituto de Investigación Biomédica de A Coruña (INIBIC). Complexo Hospitalario Universitario de A Coruña (CHUAC). SERGAS. Departamento de Medicina. Facultade de Oza. Universidade de A Coruña (UDC). As Xubias, 15006. A Coruña, Spain.
authors.forename: I
authors.initials: I
authors.lastname: Fuentes
authors.affiliation: Grupo de Terapia Celular y Medicina Regenerativa (TCMR-CHUAC). CIBER-BBN/ISCIII. Servicio de Reumatología. Instituto de Investigación Biomédica de A Coruña (INIBIC). Complexo Hospitalario Universitario de A Coruña (CHUAC). SERGAS. Departamento de Medicina. Facultade de Oza. Universidade de A Coruña (UDC). As Xubias, 15006. A Coruña, Spain.
authors.forename: J
authors.initials: J
authors.lastname: De Toro
authors.affiliation: Grupo de Proteómica-PBR2-ProteoRed/ISCIII-Servicio de Reumatologia. Instituto de Investigación Biomédica de A Coruña (INIBIC), Complexo Hospitalario Universitario de A Coruña (CHUAC), Sergas. Universidade da Coruña (UDC). As Xubias, 15006. A Coruña, España.
authors.forename: N
authors.initials: N
authors.lastname: Oreiro
authors.affiliation: Grupo Fisiopatología Endocrina, Nutricional y Médica (FENM-CHUAC). Instituto de Investigación Biomédica de A Coruña (INIBIC). Complexo Hospitalario Universitario de A Coruña (CHUAC). SERGAS. Departamento de Medicina. Facultade de Oza. Universidade de A Coruña (UDC). As Xubias, 15006. A Coruña, Spain.
authors.forename: S
authors.initials: S
authors.lastname: Sangiao-Alvarellos
authors.affiliation: Grupo de Terapia Celular y Medicina Regenerativa (TCMR-CHUAC). CIBER-BBN/ISCIII. Servicio de Reumatología. Instituto de Investigación Biomédica de A Coruña (INIBIC). Complexo Hospitalario Universitario de A Coruña (CHUAC). SERGAS. Departamento de Medicina. Facultade de Oza. Universidade de A Coruña (UDC). As Xubias, 15006. A Coruña, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Mateos
authors.affiliation: Grupo de Terapia Celular y Medicina Regenerativa (TCMR-CHUAC). CIBER-BBN/ISCIII. Servicio de Reumatología. Instituto de Investigación Biomédica de A Coruña (INIBIC). Complexo Hospitalario Universitario de A Coruña (CHUAC). SERGAS. Departamento de Medicina. Facultade de Oza. Universidade de A Coruña (UDC). As Xubias, 15006. A Coruña, Spain.
authors.forename: M C
authors.initials: MC
authors.lastname: Arufe
mesh_terms: D000375:Aging / Q000502:physiology*; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D049109:Cell Proliferation; D007553:Isotope Labeling; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D039904:Pluripotent Stem Cells / Q000166:cytology; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results; D015398:Signal Transduction; D058570:TOR Serine-Threonine Kinases / Q000378:metabolism
chemical_list: D058570:TOR Serine-Threonine Kinases