title: Hydrazide functionalized monodispersed silica microspheres: a novel probe with tunable selectivity for a versatile enrichment of phosphopeptides with different numbers of phosphorylation sites in MS analysis.
abstract: Hydrazide functionalized monodispersed silica microspheres (HFMSM) were developed for the enrichment of phosphopeptides for the first time. With the aid of the tunable selectivity of HFMSM, global enrichment or fractionation of phosphopeptides with different numbers of phosphorylation sites could be realized by a simple modulation of the concentrations of formic acid in buffers.
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China. yu.bai@pku.edu.cn.
authors.forename: Linnan
authors.initials: L
authors.lastname: Xu
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China. yu.bai@pku.edu.cn.
authors.forename: Wen
authors.initials: W
authors.lastname: Ma
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China. yu.bai@pku.edu.cn.
authors.forename: Sensen
authors.initials: S
authors.lastname: Shen
authors.affiliation: Department of Chemistry, Capital Normal University, Beijing, 100048, China.
authors.forename: Liping
authors.initials: L
authors.lastname: Li
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China. yu.bai@pku.edu.cn.
authors.forename: Yu
authors.initials: Y
authors.lastname: Bai
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China. yu.bai@pku.edu.cn.
authors.forename: Huwei
authors.initials: H
authors.lastname: Liu
mesh_terms: D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D015335:Molecular Probes; D010748:Phosphopeptides / Q000737:chemistry*; D010766:Phosphorylation; D012822:Silicon Dioxide / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D015335:Molecular Probes; D010748:Phosphopeptides; D012822:Silicon Dioxide