title: Dynamic changes of histone H3 lysine 9 following trimethylation in bovine oocytes and pre-implantation embryos.
abstract: OBJECTIVE
  We have examined dynamic changes of histone H3 lysine 9 following trimethylation (H3K9me3), the mRNA expression levels of SUV39H1 and SUV39H2 in bovine oocytes and the role in the development of in vitro fertilization (IVF) pre-implantation embryos.
  
  
  RESULTS
  There were strong H3K9me3 signals in germinal vesicle (GV) oocytes but no signals in MII oocytes. H3K9me3 signals were maintained during IVF pre-implantation embryo development. SUV39H1 and SUV39H2 showed significantly higher mRNA expression levels in GV oocytes than MII oocytes (P < 0.01). SUV39H1 showed high mRNA expression level in two-cell embryos, however, SUV39H2 showed high mRNA expression level in four-cell embryos. In other development stage, SUV39H1 and SUV39H2 showed low expression levels.
  
  
  CONCLUSIONS
  Bovine IVF pre-implantation embryos maintain strong H3K9me3 signals and SUV39H1 and SUV39H2 are highly expressed at the early development stage of pre-implantation embryos.
authors.affiliation: College of Animal Science, Jilin University, Changchun, Jilin, 130062, China. lncyzhangsheng@126.com.
authors.forename: Sheng
authors.initials: S
authors.lastname: Zhang
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun, Jilin, 130062, China. 769890578@qq.com.
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun, Jilin, 130062, China. 13604420125@163.com.
authors.forename: Congli
authors.initials: C
authors.lastname: Fan
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun, Jilin, 130062, China. tang_bo@jlu.edu.cn.
authors.forename: Bo
authors.initials: B
authors.lastname: Tang
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun, Jilin, 130062, China. zhangxuem@jlu.edu.cn.
authors.forename: Xueming
authors.initials: X
authors.lastname: Zhang
authors.affiliation: College of Animal Science, Jilin University, Changchun, Jilin, 130062, China. ziyi@jlu.edu.cn.|State & Local Joint Engineering Laboratory for Animal Models of Human Diseases, Academy of Translational Medicine, First Hospital, Jilin University, Changchun, Jilin, 130061, China. ziyi@jlu.edu.cn.
authors.forename: Ziyi
authors.initials: Z
authors.lastname: Li
mesh_terms: D000818:Animals; D002417:Cattle; D004622:Embryo, Mammalian; D047108:Embryonic Development; D005307:Fertilization in Vitro; D020869:Gene Expression Profiling; D011495:Histone-Lysine N-Methyltransferase / Q000096:biosynthesis*; D006657:Histones / Q000096:biosynthesis* / Q000378:metabolism; D008745:Methylation; D009865:Oocytes; D011499:Protein Processing, Post-Translational
chemical_list: D006657:Histones; D011495:Histone-Lysine N-Methyltransferase
keywords: Bovine oocytes; Germinal vesicle oocytes; Histone H3 lysine 9 trimethylation; In vitro fertilization; Pre-implantation embryos; SUV39H1; SUV39H2