title: Increased fat cell size: a major phenotype of subcutaneous white adipose tissue in non-obese individuals with type 2 diabetes.
abstract: OBJECTIVE
  We aimed to elucidate the impact of fat cell size and inflammatory status of adipose tissue on the development of type 2 diabetes in non-obese individuals.
  
  
  METHODS
  We characterised subcutaneous abdominal adipose tissue by examining stromal cell populations by 13 colour flow cytometry, measuring expression of adipogenesis genes in the progenitor cell fraction and determining lipolysis and adipose secretion of inflammatory proteins in 14 non-obese men with type 2 diabetes and 13 healthy controls matched for age, sex, body weight and total fat mass.
  
  
  RESULTS
  Individuals with diabetes had larger fat cells than the healthy controls but stromal cell population frequencies, adipose lipolysis and secretion of inflammatory proteins did not differ between the two groups. However, in the entire cohort fat cell size correlated positively with the ratio of M1/M2 macrophages, TNF-α secretion, lipolysis and insulin resistance. Expression of genes encoding regulators of adipogenesis and adipose morphology (BMP4, CEBPα [also known as CEBPA], PPARγ [also known as PPARG] and EBF1) correlated negatively with fat cell size.
  
  
  CONCLUSIONS
  We show that a major phenotype of white adipose tissue in non-obese individuals with type 2 diabetes is adipocyte hypertrophy, which may be mediated by an impaired adipogenic capacity in progenitor cells. Consequently, this could have an impact on adipose tissue inflammation, release of fatty acids, ectopic fat deposition and insulin sensitivity.
authors.affiliation: Lipid laboratory, Novum, D4, Department of Medicine Huddinge, Karolinska Institutet, Hälsovägen 7, 14186, Stockholm, Sweden.
authors.forename: Juan R
authors.initials: JR
authors.lastname: Acosta
authors.affiliation: Center of Hematology and Regenerative Medicine, Novum, D4, Department of Medicine Huddinge, Karolinska Institutet, Stockholm, Sweden.
authors.forename: Iyadh
authors.initials: I
authors.lastname: Douagi
authors.affiliation: Lipid laboratory, Novum, D4, Department of Medicine Huddinge, Karolinska Institutet, Hälsovägen 7, 14186, Stockholm, Sweden.
authors.forename: Daniel P
authors.initials: DP
authors.lastname: Andersson
authors.affiliation: Lipid laboratory, Novum, D4, Department of Medicine Huddinge, Karolinska Institutet, Hälsovägen 7, 14186, Stockholm, Sweden.
authors.forename: Jesper
authors.initials: J
authors.lastname: Bäckdahl
authors.affiliation: Lipid laboratory, Novum, D4, Department of Medicine Huddinge, Karolinska Institutet, Hälsovägen 7, 14186, Stockholm, Sweden.
authors.forename: Mikael
authors.initials: M
authors.lastname: Rydén
authors.affiliation: Lipid laboratory, Novum, D4, Department of Medicine Huddinge, Karolinska Institutet, Hälsovägen 7, 14186, Stockholm, Sweden.
authors.forename: Peter
authors.initials: P
authors.lastname: Arner
authors.affiliation: Lipid laboratory, Novum, D4, Department of Medicine Huddinge, Karolinska Institutet, Hälsovägen 7, 14186, Stockholm, Sweden. Jurga.Laurencikiene@ki.se.
authors.forename: Jurga
authors.initials: J
authors.lastname: Laurencikiene
mesh_terms: D017667:Adipocytes / Q000378:metabolism* / Q000473:pathology*; D052436:Adipose Tissue, White / Q000378:metabolism* / Q000473:pathology*; D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism* / Q000473:pathology*; D005260:Female; D006801:Humans; D007328:Insulin / Q000378:metabolism; D007333:Insulin Resistance / Q000502:physiology; D008066:Lipolysis / Q000502:physiology; D008264:Macrophages / Q000378:metabolism / Q000502:physiology; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000378:metabolism / Q000473:pathology; D050151:Subcutaneous Fat / Q000378:metabolism* / Q000473:pathology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D007328:Insulin; D014409:Tumor Necrosis Factor-alpha
keywords: Adipocyte hypertrophy; Non-obese; Subcutaneous adipose; Type 2 diabetes