title: Inhibition of tyrosinase activity and melanine pigmentation by 2-hydroxytyrosol.
abstract: 2-Hydroxytyrosol (2-HT), originally reported as a synthetic compound, was isolated for the first time as a fungal metabolite. 2-HT was found to inhibit mushroom tyrosinase with an IC50 value of 13.0 µmol/L. Furthermore, 2-HT dose-dependently inhibited tyrosinase activity (IC50, 32.5 µmol/L) in the cell-free extract of B16 melanoma cells and α-melanocyte stimulating hormone (α-MSH)-stimulated melanin formation in intact B16 melanoma cells.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Kitasato University, Tokyo 108-8641, Japan ; School of Pharmacy, Kitasato University, Tokyo 108-8641, Japan.
authors.forename: Ryuji
authors.initials: R
authors.lastname: Uchida
authors.affiliation: Graduate School of Pharmaceutical Sciences, Kitasato University, Tokyo 108-8641, Japan.
authors.forename: Seiko
authors.initials: S
authors.lastname: Ishikawa
authors.affiliation: Graduate School of Pharmaceutical Sciences, Kitasato University, Tokyo 108-8641, Japan ; School of Pharmacy, Kitasato University, Tokyo 108-8641, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tomoda
keywords: 2-Hydroxytyrosol; B16 melanoma cells; Melanine formation; Metarhizium sp.; Tyrosinase inhibitor