title: Evaluation of the effect of post-translational modification toward protein structure: Chemical synthesis of glycosyl crambins having either a high mannose-type or a complex-type oligosaccharide.
abstract: Glycoproteins are assembled and folded in the endoplasmic reticulum (ER) and transported to the Golgi for further processing of their oligosaccharides. During these processes, two types of oligosaccharides are used: that is, high mannose-type oligosaccharide in the ER and complex-type oligosaccharide in the Golgi. We were interested to know how two different types of oligosaccharides could influence the folding pathway or the final three-dimensional structure of the glycoproteins. For this purpose, we synthesized a new glycosyl crambin having complex-type oligosaccharide and evaluated the folding process, the final protein structure analyzed by NMR, and compared the CD spectra with previously synthesized glycosyl crambin bearing high mannose-type oligosaccharides. From our analysis, we found that the two different oligosaccharides do not influence the folding pathway in vitro and the final structure of the small glycoproteins. © 2015 Wiley Periodicals, Inc. Biopolymers (Pept Sci) 106: 446-452, 2016.
authors.affiliation: Japan Science and Technology Agency (JST), ERATO, Ito Glycotrilogy Project, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan.
authors.forename: Simone
authors.initials: S
authors.lastname: Dedola
authors.affiliation: Department of Chemistry, Graduate School of Science, Osaka University, 1-1 Machikaneyama-Cho, Toyonaka, Osaka, 560-0043, Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Izumi
authors.affiliation: Japan Science and Technology Agency (JST), ERATO, Ito Glycotrilogy Project, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan.
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Makimura
authors.affiliation: Japan Science and Technology Agency (JST), ERATO, Ito Glycotrilogy Project, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan.|RIKEN Advanced Science Institute, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan.
authors.forename: Yukishige
authors.initials: Y
authors.lastname: Ito
authors.affiliation: Japan Science and Technology Agency (JST), ERATO, Ito Glycotrilogy Project, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan.|Department of Chemistry, Graduate School of Science, Osaka University, 1-1 Machikaneyama-Cho, Toyonaka, Osaka, 560-0043, Japan.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Kajihara
mesh_terms: D006023:Glycoproteins / Q000096:biosynthesis / Q000737:chemistry; D008358:Mannose / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D009844:Oligosaccharides / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D010940:Plant Proteins / Q000096:biosynthesis / Q000737:chemistry; D011499:Protein Processing, Post-Translational
chemical_list: D006023:Glycoproteins; D009844:Oligosaccharides; D010940:Plant Proteins; C024934:crambin protein, Crambe abyssinica; D008358:Mannose
keywords: glycopeptide; glycoprotein; protein conformation