title: Multifunctional Bioconjugate for Cancer Cell-Targeted Theranostics.
abstract: Cancer cell-targeted imaging and drug delivery remain a challenge for precise cancer theranostics. MUC1 is a large transmembrane glycoprotein that may potentially serve as a target for cancer theranostics. Herein, using a MUC1-targeting aptamer (APT) as the "warhead", we rationally designed and constructed a hybrid nanoparticle 1-NPs-QDs-hAPT (Vehicle) that could be applied for MUC1-targeted cell uptake and imaging. By intercalating different Vehicle amounts with the anticancer drug doxorubicin (DOX), we obtained the multifunctional bioconjugate Vehicle-DOX with a maximized drug payload and DOX fluorescence quenching capability. Confocal microscopy cell imaging indicated that Vehicle-DOX could be used to track MUC1-targeted drug release. A cytotoxicity study indicated that Vehicle-DOX could be applied for MUC1-targeted cytotoxicity. We anticipate that our multifunctional bioconjugate Vehicle-DOX could be applied for in vivo tumor-targeted theranostics.
authors.affiliation: CAS Key Laboratory of Soft Matter Chemistry, National Synchrotron Radiation Laboratory, Department of Chemistry, University of Science and Technology of China , 96 Jinzhai Road, Hefei, Anhui 230026, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Du
authors.affiliation: CAS Key Laboratory of Soft Matter Chemistry, National Synchrotron Radiation Laboratory, Department of Chemistry, University of Science and Technology of China , 96 Jinzhai Road, Hefei, Anhui 230026, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: School of Life Sciences, University of Science and Technology of China , Hefei, Anhui 230027, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
authors.affiliation: CAS Key Laboratory of Soft Matter Chemistry, National Synchrotron Radiation Laboratory, Department of Chemistry, University of Science and Technology of China , 96 Jinzhai Road, Hefei, Anhui 230026, China.
authors.forename: Yusi
authors.initials: Y
authors.lastname: Cui
authors.affiliation: Department of Nuclear Medicine, The First Affiliated Hospital of Anhui Medical University , 218 Jixi Road, Hefei, Anhui 230022, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Nuclear Medicine, The First Affiliated Hospital of Anhui Medical University , 218 Jixi Road, Hefei, Anhui 230022, China.
authors.forename: Huiqin
authors.initials: H
authors.lastname: Xu
authors.affiliation: CAS Key Laboratory of Soft Matter Chemistry, National Synchrotron Radiation Laboratory, Department of Chemistry, University of Science and Technology of China , 96 Jinzhai Road, Hefei, Anhui 230026, China.
authors.forename: Gaolin
authors.initials: G
authors.lastname: Liang
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000008:administration & dosage* / Q000494:pharmacology; D052157:Aptamers, Nucleotide / Q000378:metabolism*; D001940:Breast / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D001943:Breast Neoplasms / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D004317:Doxorubicin / Q000008:administration & dosage* / Q000494:pharmacology; D016503:Drug Delivery Systems; D005260:Female; D006801:Humans; D061986:MCF-7 Cells; D018396:Mucin-1 / Q000378:metabolism*; D053758:Nanoparticles / Q000378:metabolism* / Q000648:ultrastructure; D061848:Optical Imaging; D000068936:Theranostic Nanomedicine
chemical_list: D000903:Antibiotics, Antineoplastic; D052157:Aptamers, Nucleotide; D018396:Mucin-1; D004317:Doxorubicin