title: Comparison of bacterial growth in sonication fluid cultures with periprosthetic membranes and with cultures of biopsies for diagnosing periprosthetic joint infection.
abstract: Total joint arthroplasty is a common operation worldwide with infection rates between 1% and 3%. In cases of suspected periprosthetic joint infection, it is very challenging to rule out the causative microorganisms. In this study, we compared the appearance of periprosthetic membranes with the microbiological results obtained from cultures of sonication fluid and the correlation between classical microbiological cultures and cultures of sonication fluid. The results confirmed a strong correlation of bacterial growth in sonication fluid cultures with bacterial growth in classical microbiological cultures. Most importantly, however, our study documented a highly significant correlation of periprosthetic membranes typical for periprosthetic joint infection (PJI) with bacterial growth in sonication fluid. Sonication fluid cultures yielded a better sensitivity than tissue cultures (72.34-60.87%). These 3 methods are useful tools in diagnosing PJIs, and even more, sonication fluid cultures should be included in the diagnostic path of PJI.
authors.affiliation: Institute of Medical Microbiology, Immunology and Parasitology, University Hospital Bonn, Sigmund-Freud-Str. 25, D-53127 Bonn, Germany. Electronic address: hischebeth@microbiology-bonn.de.
authors.forename: Gunnar T R
authors.initials: GT
authors.lastname: Hischebeth
authors.affiliation: Department of Orthopaedics and Trauma Surgery, University Hospital Bonn, Sigmund-Freud-Str. 25, D-53127 Bonn, Germany.
authors.forename: Thomas M
authors.initials: TM
authors.lastname: Randau
authors.affiliation: Institute of Medical Microbiology, Immunology and Parasitology, University Hospital Bonn, Sigmund-Freud-Str. 25, D-53127 Bonn, Germany.
authors.forename: Ernst
authors.initials: E
authors.lastname: Molitor
authors.affiliation: Department of Orthopaedics and Trauma Surgery, University Hospital Bonn, Sigmund-Freud-Str. 25, D-53127 Bonn, Germany.
authors.forename: Matthias D
authors.initials: MD
authors.lastname: Wimmer
authors.affiliation: Institute of Medical Microbiology, Immunology and Parasitology, University Hospital Bonn, Sigmund-Freud-Str. 25, D-53127 Bonn, Germany.
authors.forename: Achim
authors.initials: A
authors.lastname: Hoerauf
authors.affiliation: Institute of Medical Microbiology, Immunology and Parasitology, University Hospital Bonn, Sigmund-Freud-Str. 25, D-53127 Bonn, Germany; Division of Microbiology, Paul-Ehrlich-Institute, Paul-Ehrlich-Str 51-59, D-63225 Langen, Germany.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Bekeredjian-Ding
authors.affiliation: Department of Orthopaedics and Trauma Surgery, University Hospital Bonn, Sigmund-Freud-Str. 25, D-53127 Bonn, Germany.
authors.forename: Sascha
authors.initials: S
authors.lastname: Gravius
mesh_terms: D001424:Bacterial Infections / Q000175:diagnosis*; D001431:Bacteriological Techniques / Q000379:methods; D001706:Biopsy; D006801:Humans; D007596:Joints / Q000382:microbiology*; D016459:Prosthesis-Related Infections / Q000175:diagnosis*; D012189:Retrospective Studies; D012680:Sensitivity and Specificity; D013010:Sonication; D013048:Specimen Handling / Q000379:methods*; D013582:Synovial Fluid / Q000382:microbiology*
keywords: Histopathology; PJI; Periprosthetic membranes; Sonication