title: Effect of miR-503 Down-Regulation on Growth and Invasion of Esophagus Carcinoma and Related Immune Function.
abstract: BACKGROUND MicroRNA (miR) has been proved to be an important biomarker for tumors because it can regulate occurrence, progression, invasion, and metastasis of cancer. A previous study has shown the involvement of miR-503 in multiple gastrointestinal tumors. Its detailed role and immune regulatory function in esophagus carcinoma, however, remains unknown. This study thus investigated the effect of miR-503 in regulating growth, proliferation, and invasion of esophagus cancer and its influence on cytokine secretion. MATERIAL AND METHODS Esophagus carcinoma cell line EC9706 and normal esophageal epithelial cell line HEEC were transfected with miR-503 inhibitor. MTT assay was used to quantify the cell proliferation, and a Transwell chamber was used to evaluate cell invasion. Release of cytokines, including interleukin-2 (IL-2), IL-4, IL-10, and interferon-γ (IFN-γ), was measured by enzyme-linked immunosorbent assay (ELISA). RESULTS MiR-503 expression was significantly elevated in esophagus carcinoma cells (p<0.05). The specific inhibition of miR-503 expression remarkably suppressed proliferation and invasion of tumor cells. It can also down-regulated IL-2 and IFN-γ expression and facilitate secretion of IL-4 and IL-10 when compared to the control group (p<0.05 in all ceases). CONCLUSIONS The inhibition of miR-503 can effectively inhibit tumor progression and improve immune function, suggesting its potency as a novel drug target for esophagus cancer treatment.
authors.affiliation: Department of Thoracic Surgery, The Central Hospital of Wuhan, Wuhan, Hubei, China (mainland).
authors.forename: Ke
authors.initials: K
authors.lastname: Zhao
authors.affiliation: Department of Thoracic Surgery, The Central Hospital of Wuhan, Wuhan, Hubei, China (mainland).
authors.forename: Bao-Jun
authors.initials: BJ
authors.lastname: Chen
authors.affiliation: Department of Thoracic Surgery, The Central Hospital of Wuhan, Wuhan, Hubei, China (mainland).
authors.forename: Zhi-Guo
authors.initials: ZG
authors.lastname: Chen
authors.affiliation: Department of Thoracic Surgery, The Central Hospital of Wuhan, Wuhan, Hubei, China (mainland).
authors.forename: Yong-Jian
authors.initials: YJ
authors.lastname: Zhang
authors.affiliation: Department of Thoracic Surgery, The Central Hospital of Wuhan, Wuhan, Hubei, China (mainland).
authors.forename: Di
authors.initials: D
authors.lastname: Xu
authors.affiliation: Department of Thoracic Surgery, The Central Hospital of Wuhan, Wuhan, Hubei, China (mainland).
authors.forename: Qi
authors.initials: Q
authors.lastname: Liu
mesh_terms: D018267:Carcinoma, Acinar Cell / Q000235:genetics; D016022:Case-Control Studies; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D016207:Cytokines / Q000276:immunology / Q000378:metabolism; D015536:Down-Regulation / Q000187:drug effects; D004938:Esophageal Neoplasms / Q000235:genetics* / Q000276:immunology / Q000378:metabolism / Q000628:therapy*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007371:Interferon-gamma / Q000276:immunology / Q000378:metabolism; D007378:Interleukins / Q000276:immunology / Q000378:metabolism; D035683:MicroRNAs / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D009841:Oligonucleotides / Q000008:administration & dosage / Q000235:genetics; D011897:Random Allocation; D053300:Tumor Necrosis Factor Ligand Superfamily Member 13 / Q000378:metabolism
chemical_list: D016207:Cytokines; D007378:Interleukins; C545473:MIRN503 microRNA, human; D035683:MicroRNAs; D009841:Oligonucleotides; D053300:Tumor Necrosis Factor Ligand Superfamily Member 13; D007371:Interferon-gamma