title: Antiretroviral Therapy Fails to Restore Levels of HIV-1 Restriction miRNAs in PBMCs of HIV-1-infected MSM.
abstract: A number of cellular microRNAs (miRNAs) have been identified to have the ability to inhibit HIV-1 replication. In this study, we examined the impact of combination antiretroviral therapy (cART) on the expression of HIV-1 restriction miRNAs in peripheral blood mononuclear cells of HIV-1-infected men who have sex with men (MSM). Compared with male healthy donors, HIV-infected MSM had significantly lower levels of 9 HIV-1 restriction miRNAs. The treatment of HIV-1-infected MSM with cART, however, failed to restore the levels of these miRNAs in peripheral blood mononuclear cells. These observations suggest that the suppression of the cellular restriction miRNAs by HIV-1 may attribute to the virus latency during cART.
authors.affiliation: From the Department of Virology, Wuhan Centers for Disease Prevention & Control (M-QL, W-HK, J-SP, FW, Z-RZ, LT, WZ); Wuhan Institute of Dermatology and Venereology, Wuhan (MZ, H-YQ); Central Laboratory of the Fourth Affiliated Hospital in Xiangyang, College of Basic Medical Sciences, Hubei Key Laboratory of Wudang Local Chinese Medicine Research, Hubei University of Medicine, Shiyan (MS); State Key Laboratory of Virology, Wuhan University, Wuhan, China (J-GW, W-ZH); and Department of Pathology and Laboratory Medicine, Temple University School of Medicine, Philadelphia, PA (W-ZH).
authors.forename: Man-Qing
authors.initials: MQ
authors.lastname: Liu
authors.forename: Min
authors.initials: M
authors.lastname: Zhao
authors.forename: Wen-Hua
authors.initials: WH
authors.lastname: Kong
authors.forename: Jin-Song
authors.initials: JS
authors.lastname: Peng
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.forename: Hong-Yan
authors.initials: HY
authors.lastname: Qiu
authors.forename: Ze-Rong
authors.initials: ZR
authors.lastname: Zhu
authors.forename: Li
authors.initials: L
authors.lastname: Tang
authors.forename: Ming
authors.initials: M
authors.lastname: Sang
authors.forename: Jian-Guo
authors.initials: JG
authors.lastname: Wu
authors.forename: Wen-Zhe
authors.initials: WZ
authors.lastname: Ho
authors.forename: Wang
authors.initials: W
authors.lastname: Zhou
mesh_terms: D000328:Adult; D019380:Anti-HIV Agents / Q000494:pharmacology* / Q000627:therapeutic use; D023241:Antiretroviral Therapy, Highly Active; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D015658:HIV Infections / Q000097:blood / Q000188:drug therapy* / Q000276:immunology / Q000821:virology; D015497:HIV-1 / Q000502:physiology; D018451:Homosexuality, Male; D006801:Humans; D007113:Immunity, Innate / Q000187:drug effects; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D035683:MicroRNAs / Q000097:blood* / Q000276:immunology; D008875:Middle Aged; D016896:Treatment Outcome; D014779:Virus Replication / Q000235:genetics
chemical_list: D019380:Anti-HIV Agents; D015415:Biomarkers; D035683:MicroRNAs