title: [Effect of RbAp48 knockdown on migration and invasion of human cervical cancer cell line MS751 in vitro].
abstract: OBJECTIVE
  To investigate the effect of RbAp48 knockdown on the migration and invasion of human cervical cancer cells and explore the mechanism.
  
  
  METHODS
  A small interference RNA (siRNA) was used to knock down the expression of RbAp48 in MS751 cells. The changes in cell migration and invasion were evaluated using wound healing assay and Transwell assay, respectively, and the expressions of RbAp48, vimentin, N-cadherin, E-cadherin, Snail, Twist, MMP-2 and TIMP-2 were determined with Western blotting.
  
  
  RESULTS
  After siRNA-mediated RbAp48 knockdown, MS751 cells showed a significantly reduced expression of RbAp48 with significantly suppressed cell migration and invasion (P<0.01). RbAp48 knockdown induced obvious down-regulation of the expressions of interstitial cell phenotype proteins vimentin, N-cadherin, and MMP-2 and up-regulation of epithelial cell phenotype proteins E-cadherin and TIMP-2, suggesting the inhibition of epithelial- mesenchymal transition of the cells. The expressions of Snail and Twist were significantly down-regulated in the cells following RbAp48 knockdown.
  
  
  CONCLUSIONS
  Knockdown of RbAp48 can significantly inhibit epithelial-mesenchymal transition and suppress the migration and invasion of cervical cancer cell line MS751, the mechanism of which may involve the down-regulation of Snail and Twist expressions.
authors.affiliation: School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, China.E-mail: 731745385@qq.com.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Zhong
authors.forename: Xurui
authors.initials: X
authors.lastname: Yang
authors.forename: Meiqing
authors.initials: M
authors.lastname: Mai
authors.forename: Dandan
authors.initials: D
authors.lastname: Wang
authors.forename: Lin
authors.initials: L
authors.lastname: Lv
authors.forename: Jinjun
authors.initials: J
authors.lastname: Rao
mesh_terms: D015703:Antigens, CD / Q000378:metabolism; D015820:Cadherins / Q000378:metabolism; D045744:Cell Line, Tumor; D002465:Cell Movement; D015536:Down-Regulation; D058750:Epithelial-Mesenchymal Transition; D005260:Female; D055785:Gene Knockdown Techniques; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D009361:Neoplasm Invasiveness; D009687:Nuclear Proteins / Q000378:metabolism; D034741:RNA, Small Interfering; D056505:Retinoblastoma-Binding Protein 4 / Q000235:genetics*; D000071250:Snail Family Transcription Factors; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism; D051879:Twist-Related Protein 1 / Q000378:metabolism; D015854:Up-Regulation; D002583:Uterine Cervical Neoplasms / Q000473:pathology*; D014746:Vimentin / Q000378:metabolism
chemical_list: D015703:Antigens, CD; C513384:CDH1 protein, human; C050361:CDH2 protein, human; D015820:Cadherins; D009687:Nuclear Proteins; C082637:RBBP4 protein, human; D034741:RNA, Small Interfering; D056505:Retinoblastoma-Binding Protein 4; D000071250:Snail Family Transcription Factors; C560823:TIMP2 protein, human; C103173:TWIST1 protein, human; D014157:Transcription Factors; D051879:Twist-Related Protein 1; D014746:Vimentin; D019716:Tissue Inhibitor of Metalloproteinase-2; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2