title: Immunohistochemical localization of Nox in mouse circumvallate papillae.
abstract: Recently it has been reported that reactive oxygen species plays an important role in several physiological processes. Reactive oxygen species are generated by reactive oxygen-synthesizing enzymes (Nox). We immunohistochemically investigated expression and localization of the Nox family in a mouse circumvallate papillae. In the epithelium of the circumvallate papilla, Nox 1, 2, 3, and 4, Noxo1, and Noxa1 were expressed. In the circumvallate papilla, Nox2 was more weakly expressed in the lateral than in the upper part, and Nox3 was not expressed. In the taste buds, Nox 1, 2, 3, and 4, Noxo1, and Noxa1 were expressed; the Nox expression pattern varied with the cell type. In type II cells, Nox 1, 3, and 4, Noxo1, and Noxa1 were expressed. In type III cells, Nox2, besides Nox 1, 3, and 4, Noxo1, and Noxa1, were specifically expressed, unlike in other taste bud cells. In the mouse circumvallate papilla, Nox is always expressed, suggesting the generation of reactive oxygen species. Of note, all cells comprising taste buds expressed Nox, with each showing a specific Nox expression pattern.
authors.affiliation: Department of Cell Biology and Oral Histology, Ohu University, Graduate School of Dentistry, 31-1 Misumido, Tomita-machi, Koriyama 963-8611, Japan.
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Kashiwabara
authors.affiliation: Division of Oral Histology, Department of Morphological Biology, Ohu University School of Dentistry, 31-1 Misumido, Tomita-machi, Koriyama 963-8611, Japan.
authors.forename: Kimiharu
authors.initials: K
authors.lastname: Ambe
authors.affiliation: Division of Oral Histology, Department of Morphological Biology, Ohu University School of Dentistry, 31-1 Misumido, Tomita-machi, Koriyama 963-8611, Japan.
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Nakagawa
authors.affiliation: Division of Oral Histology, Department of Morphological Biology, Ohu University School of Dentistry, 31-1 Misumido, Tomita-machi, Koriyama 963-8611, Japan. Electronic address: hi-watanabe@den.ohu-u.ac.jp.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Watanabe
mesh_terms: D000818:Animals; D004848:Epithelium / Q000378:metabolism* / Q000473:pathology; D007150:Immunohistochemistry / Q000379:methods; D008810:Mice, Inbred C57BL; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism*; D019255:NADPH Oxidases / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013650:Taste Buds / Q000378:metabolism* / Q000473:pathology
chemical_list: D017382:Reactive Oxygen Species; D009247:NADH, NADPH Oxidoreductases; D019255:NADPH Oxidases
keywords: Circumvallate papillae; Immunohistochemistry; NADPH oxidase; Nox; Reactive oxygen species; Taste bud