title: An optical sensing composite for cysteine detection using up-conversion nanoparticles and a rhodamine-derived chemosensor: Construction, characterization, photophysical feature and sensing performance.
abstract: In this paper, we reported an optical sensing composite for cysteine detection. A chemosensor derived from rhodamine 6G was synthesized and characterized. To minimize its photobleaching, up-conversion nanocrystals β-NaYF4:Yb(3+)/Er(3+) were prepared and modified with α-cyclodextrin, serving as excitation host. Under 980nm laser excitation, emission of these up-conversion nanocrystals overlapped well with the absorption of our chemosensor. Energy transfer between them was analyzed and confirmed by emission decay analysis. Job's analysis suggested that the complexation equilibrium between our chemosensor and cysteine was a simple one with binding stoichiometry of 1:1. A sensing system was constructed with up-conversion nanocrystals (modified with α-cyclodextrin) and this chemosensor. Emission "turn-on" effect was observed only for cysteine but immune to other competing amino acids and thiols, showing a good selectivity.
authors.affiliation: School of Life Science, Changchun Normal University, Changchun 130032, China.
authors.forename: Song
authors.initials: S
authors.lastname: Kai
authors.affiliation: School of Life Science, Changchun Normal University, Changchun 130032, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Cheng-Wen
authors.affiliation: School of Life Science, Changchun Normal University, Changchun 130032, China.
authors.forename: Ding
authors.initials: D
authors.lastname: Yi-Nan
authors.affiliation: School of Life Science, Changchun Normal University, Changchun 130032, China.
authors.forename: Luan
authors.initials: L
authors.lastname: Tian
authors.affiliation: School of Life Science, Changchun Normal University, Changchun 130032, China.
authors.forename: Wang
authors.initials: W
authors.lastname: Guang-Ye
authors.affiliation: School and Life Science, Northeast Normal University, Changchun 130024, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Jing-Mei
authors.affiliation: School and Life Science, Northeast Normal University, Changchun 130024, China; Key Laboratory of Grain and Oil Processing of Jilin Province, Jilin Business and Technology College, Changchun 130507, China. Electronic address: guolq948@163.com.
authors.forename: Guo
authors.initials: G
authors.lastname: Li-Quan
mesh_terms: D003545:Cysteine / Q000032:analysis*; D004871:Erbium / Q000737:chemistry*; D005459:Fluorides / Q000737:chemistry*; D049408:Luminescent Agents / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D012235:Rhodamines / Q000737:chemistry*; D015018:Ytterbium / Q000737:chemistry*; D015019:Yttrium / Q000737:chemistry*; D047391:alpha-Cyclodextrins / Q000737:chemistry
chemical_list: D049408:Luminescent Agents; D012235:Rhodamines; D047391:alpha-Cyclodextrins; C525362:sodium yttriumtetrafluoride; C026188:rhodamine 6G; D015019:Yttrium; D004871:Erbium; D003545:Cysteine; D015018:Ytterbium; D005459:Fluorides; C032613:alpha-cyclodextrin
keywords: Cysteine; Rhodamine chemosensor; Sensing; Up-conversion nanoparticles