title: Inhibitory effect of sesquiterpene lactones and the sesquiterpene alcohol aromadendrane-4β,10α-diol on memory impairment in a mouse model of Alzheimer.
abstract: Alzheimer's disease (AD), a progressive neurodegenerative disorder of the aged brain with no known cause or cures, has become a major medical and social problem for industrialized countries. Cerebral deposition of amyloid-β peptide (Aβ) is a critical feature of AD. The use of medicinal plants as an alternative form of prevention, or even as a possible treatment of AD, is therefore interesting areas of research. Sesquiterpene lactones and a sesquiterpene alcohol are compounds found in H. brasiliense that have several anti-oxidative and anti-inflammatory effects. In the present study, we investigated whether these compounds have neuroprotective effects in an amyloid-β peptide-induced Alzheimer's disease mouse model. Mice were injected with Aβ1-42 peptide intracerebroventricularly and were subsequently injected (i.c.v.) with 1µg/site of IGM-A (15-acetoxy-isogermafurenolide), IGM-H (15-hydroxy-isogermafurenolide), PDA (Podoandin), EHP (1,2-epoxy-10α-hydroxy-podoandin), HDS (13-hydroxy-8,9-dehydroshizukanolide), and ARD (aromadendrane-4β,10α-diol). Seven days after treatments the animals had their memory tested in the inhibitory avoidance. After the behavioral testing of animals the brains were removed and subjected to biochemical tests for oxidative stress. The results showed that ARD, HDS and PDA significantly ameliorated the Aβ1-42 peptide-induced memory impairment in the passive avoidance task (P<0.05). In addition, GSH activity was increased while the TBARS levels were decreased by treatment with these compounds. These results suggest that these compounds inhibit the cognitive deficit of animals induced peptide amyloid and may be potential candidates for Alzheimer's disease therapy.
authors.affiliation: Programa de Pós Graduação em Farmácia, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil.
authors.forename: Solomon K S
authors.initials: SK
authors.lastname: Amoah
authors.affiliation: Núcleo de Investigações Químico Farmacêuticas (NIQFAR), Universidade do Vale do Itajaí, UNIVALI, Itajaí, Santa Catarina, Brazil.
authors.forename: Maria Tereza
authors.initials: MT
authors.lastname: Dalla Vecchia
authors.affiliation: Núcleo de Investigações Químico Farmacêuticas (NIQFAR), Universidade do Vale do Itajaí, UNIVALI, Itajaí, Santa Catarina, Brazil.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Pedrini
authors.affiliation: Núcleo de Investigações Químico Farmacêuticas (NIQFAR), Universidade do Vale do Itajaí, UNIVALI, Itajaí, Santa Catarina, Brazil.
authors.forename: Gabriela Lazzarotto
authors.initials: GL
authors.lastname: Carnhelutti
authors.affiliation: Programa de Pós Graduação em Ciências Farmacêuticas, Universidade do Vale do Itajaí , UNIVALI, Itajaí, SC, Brazil.
authors.forename: Ana Elisa
authors.initials: AE
authors.lastname: Gonçalves
authors.affiliation: Programa de Pós Graduação em Ciências Farmacêuticas, Universidade do Vale do Itajaí , UNIVALI, Itajaí, SC, Brazil.
authors.forename: Diogo Adolfo
authors.initials: DA
authors.lastname: Dos Santos
authors.affiliation: Programa de Pós Graduação em Farmácia, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil. Electronic address: maique.biavatti@ufsc.br.
authors.forename: Maique W
authors.initials: MW
authors.lastname: Biavatti
authors.affiliation: Programa de Pós Graduação em Ciências Farmacêuticas, Universidade do Vale do Itajaí , UNIVALI, Itajaí, SC, Brazil.
authors.forename: Márcia Maria
authors.initials: MM
authors.lastname: de Souza
mesh_terms: D000544:Alzheimer Disease / Q000150:complications*; D016229:Amyloid beta-Peptides / Q000378:metabolism; D000818:Animals; D001362:Avoidance Learning / Q000187:drug effects; D001522:Behavior, Animal / Q000187:drug effects; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D004195:Disease Models, Animal; D019684:Magnoliopsida / Q000737:chemistry; D008297:Male; D018782:Maze Learning / Q000187:drug effects; D008569:Memory Disorders / Q000150:complications* / Q000188:drug therapy* / Q000378:metabolism / Q000503:physiopathology; D051379:Mice; D018696:Neuroprotective Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D018384:Oxidative Stress / Q000187:drug effects; D010446:Peptide Fragments / Q000378:metabolism; D018515:Plant Leaves / Q000737:chemistry; D012717:Sesquiterpenes / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D045789:Sesquiterpenes, Guaiane
chemical_list: D016229:Amyloid beta-Peptides; D018696:Neuroprotective Agents; D010446:Peptide Fragments; D012717:Sesquiterpenes; D045789:Sesquiterpenes, Guaiane; C075222:amyloid beta-protein (1-42); C519959:aromadendrane; C543650:aromadendranediol
keywords: Alzheimer; Hedyosmum brasiliense; Sesquiterpene alcohol; Sesquiterpene lactones