title: Apoptotic Effects of Cordycepin Through the Extrinsic Pathway and p38 MAPK Activation in Human Glioblastoma U87MG Cells.
abstract: We first demonstrated that cordycepin inhibited cell growth and triggered apoptosis in U87MG cells with wild-type p53, but not in T98G cells with mutant-type p53. Western blot data revealed that the levels of procaspase-8, -3, and Bcl-2 were downregulated in cordycepintreated U87MG cells, whereas the levels of Fas, FasL, Bak, cleaved caspase-3, -8, and cleaved PARP were upregulated, indicating that cordycepin induces apoptosis by activating the death receptor-mediated pathway in U87MG cells. Cordycepin-induced apoptosis could be suppressed by only SB203580, a p38 MAPK-specific inhibitor. These results suggest that cordycepin triggered apoptosis in U87MG cells through p38 MAPK activation and inhibition of the Akt survival pathway.
authors.affiliation: Department of Medicinal Biotechnology, College of Health Sciences, Dong-A University, Busan 49315, Republic of Korea.|Department of Clinical Research, Dongnam Institute of Radiological & Medical Sciences Research Center, Busan 46033, Republic of Korea.
authors.forename: Ji-Sue
authors.initials: JS
authors.lastname: Baik
authors.affiliation: Department of Medicinal Biotechnology, College of Health Sciences, Dong-A University, Busan 49315, Republic of Korea.
authors.forename: Seo-Won
authors.initials: SW
authors.lastname: Mun
authors.affiliation: Department of Medicinal Biotechnology, College of Health Sciences, Dong-A University, Busan 49315, Republic of Korea.
authors.forename: Kyoung-Sook
authors.initials: KS
authors.lastname: Kim
authors.affiliation: Department of Medicinal Biotechnology, College of Health Sciences, Dong-A University, Busan 49315, Republic of Korea.|Department of Clinical Research, Dongnam Institute of Radiological & Medical Sciences Research Center, Busan 46033, Republic of Korea.
authors.forename: Shin-Ji
authors.initials: SJ
authors.lastname: Park
authors.affiliation: Department of Medicinal Biotechnology, College of Health Sciences, Dong-A University, Busan 49315, Republic of Korea.
authors.forename: Hyun-Kyoung
authors.initials: HK
authors.lastname: Yoon
authors.affiliation: Department of Medicinal Biotechnology, College of Health Sciences, Dong-A University, Busan 49315, Republic of Korea.
authors.forename: Dong-Hyun
authors.initials: DH
authors.lastname: Kim
authors.affiliation: Department of Clinical Pharmacology & Therapeutics, College of Medicine, Dong-A University, Busan 49201, Republic of Korea.
authors.forename: Min-Kyu
authors.initials: MK
authors.lastname: Park
authors.affiliation: Molecular and Cellular Glycobiology Unit, Department of Biological Sciences, SungKyunKwan University, Kyunggi-Do 16419, Republic of Korea.
authors.forename: Cheorl-Ho
authors.initials: CH
authors.lastname: Kim
authors.affiliation: Department of Medicinal Biotechnology, College of Health Sciences, Dong-A University, Busan 49315, Republic of Korea.
authors.forename: Young-Choon
authors.initials: YC
authors.lastname: Lee
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D053181:Caspase 8 / Q000378:metabolism; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects*; D003839:Deoxyadenosines / Q000494:pharmacology*; D053222:Fas Ligand Protein / Q000378:metabolism; D005909:Glioblastoma; D006801:Humans; D015398:Signal Transduction; D051028:bcl-2-Associated X Protein / Q000235:genetics / Q000378:metabolism; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: D000935:Antifungal Agents; D003839:Deoxyadenosines; C505661:FASLG protein, human; D053222:Fas Ligand Protein; D051028:bcl-2-Associated X Protein; D048051:p38 Mitogen-Activated Protein Kinases; D053148:Caspase 3; D053181:Caspase 8; C058120:cordycepin
keywords: Cordycepin; U87MG cell; apoptosis; extrinsic pathway; p38 MAPK