title: Use of Reporter Genes to Analyze Estrogen Response: The Transgenic Zebrafish Model.
abstract: In vivo models to detect estrogenic compounds are very valuable for screening for endocrine disruptors. Here we describe the use of transgenic estrogen reporter zebrafish as an in vivo model for identification of estrogenic properties of compounds. Live imaging of these transgenic fish provides knowledge of estrogen receptor specificity of different ligands as well as dynamics of estrogen signaling. Coupled to image analysis, the model can provide quantitative dose-response information on estrogenic activity of chemical compounds.
authors.affiliation: Department of Pharmacology and Toxicology, University of Alabama at Birmingham, Birmingham, AL, USA.
authors.forename: Daniel A
authors.initials: DA
authors.lastname: Gorelick
authors.affiliation: Center for Nuclear Receptors and Cell Signaling, Department of Biology and Biochemistry, University of Houston, 4800 Calhoun Rd., Houston, TX, 77004, USA.
authors.forename: Caroline Lucia
authors.initials: CL
authors.lastname: Pinto
authors.affiliation: Center for Molecular Toxicology and Carcinogenesis, Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, PA, USA.|DuPont Haskell Global Centers for Health and Environmental Sciences, Newark, DE, USA.
authors.forename: Ruixin
authors.initials: R
authors.lastname: Hao
authors.affiliation: Center for Nuclear Receptors and Cell Signaling, Department of Biology and Biochemistry, University of Houston, 4800 Calhoun Rd., Houston, TX, 77004, USA. mbondessonbolin@uh.edu.
authors.forename: Maria
authors.initials: M
authors.lastname: Bondesson
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D004305:Dose-Response Relationship, Drug; D004625:Embryo, Nonmammalian / Q000187:drug effects / Q000378:metabolism; D052244:Endocrine Disruptors / Q000494:pharmacology*; D004965:Estrogen Antagonists / Q000494:pharmacology; D004967:Estrogens / Q000494:pharmacology*; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics* / Q000378:metabolism; D008024:Ligands; D008856:Microscopy, Fluorescence; D011960:Receptors, Estrogen / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D020218:Response Elements; D015398:Signal Transduction / Q000187:drug effects; D013997:Time Factors; D057188:Workflow; D015027:Zebrafish / Q000196:embryology / Q000235:genetics* / Q000378:metabolism
chemical_list: D052244:Endocrine Disruptors; D004965:Estrogen Antagonists; D004967:Estrogens; D008024:Ligands; D011960:Receptors, Estrogen; D049452:Green Fluorescent Proteins
keywords: Estrogen; Fluorescence microscopy; Image analysis; Transgenic zebrafish; Xenoestrogens