title: Glaciimonas frigoris sp. nov., a psychrophilic bacterium isolated from ancient Siberian permafrost sediment, and emended description of the genusGlaciimonas.
abstract: The bacterial strain N1-38Twas isolated from ancient Siberian permafrost sediment. The strain was Gram-reaction-negative, motile by gliding, rod-shaped and psychrophilic, and showed good growth over a temperature range of - 5 to 25 °C. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain N1-38Twas most closely related to members of the genusGlaciimonasand shared the highest 16S rRNA gene sequence similarities with the type strains ofGlaciimonas alpina(99.3 %),Glaciimonas immobilis(98.9 %) andGlaciimonas singularis(96.5 %). The predominant cellular fatty acids of strain N1-38Twere summed feature 3 (C16 : 1ω7cand/or iso-C15 : 02-OH), C16 : 0and C18 : 1ω7c. The major respiratory quinone was ubiquinone 8 and the major polar lipids were phosphatidylethanolamine and diphosphatidylglycerol. The genomic DNA G+C content was 53.0 mol%. Combined data of phenotypic, phylogenetic and DNA-DNA relatedness studies demonstrated that strain N1-38Trepresents a novel species of the genusGlaciimonas, for which the nameGlaciimonas frigorissp. nov. is proposed. The type strain is N1-38T( = LMG 28868T= CCOS 838T). An emended description of the genusGlaciimonasis also provided.
authors.affiliation: Institute of Microbiology, University of Innsbruck, Technikerstrasse 25, 6020 Innsbruck, Austria.
authors.forename: Rosa
authors.initials: R
authors.lastname: Margesin
authors.affiliation: Institute of Oceanology, Chinese Academy of Sciences, 266071, Qingdao, PRChina.
authors.forename: De-Chao
authors.initials: DC
authors.lastname: Zhang
authors.affiliation: Institute of Biotechnology, Zurich University of Applied Sciences, Life Sciences & Facility Management, 8820 Waedenswil, Switzerland.
authors.forename: David
authors.initials: D
authors.lastname: Frasson
authors.affiliation: Moscow University, Geology Faculty, 1 Leninskye Gory, Moscow, 119899, Russia.
authors.forename: Anatoli
authors.initials: A
authors.lastname: Brouchkov