title: Structural studies of novel glycoconjugates from polymerized allergens (allergoids) and mannans as allergy vaccines.
abstract: Immunotherapy for treating IgE-mediated allergies requires high doses of the corresponding allergen. This may result in undesired side effects and, to avoid them, hypoallergenic allergens (allergoids) polymerized with glutaraldehyde are commonly used. Targeting allergoids to dendritic cells to enhance cell uptake may result in a more effective immunotherapy. Allergoids coupled to yeast mannan, as source of polymannoses, would be suitable for this purpose, since mannose-binding receptors are expressed on these cells. Conventional conjugation procedures of mannan to proteins use oxidized mannan to release reactive aldehydes able to bind to free amino groups in the protein; yet, allergoids lack these latter because their previous treatment with glutaraldehyde. The aim of this study was to obtain allergoids conjugated to mannan by an alternative approach based on just glutaraldehyde treatment, taking advantage of the mannoprotein bound to the polymannose backbone. Allergoid-mannan glycoconjugates were produced in a single step by treating with glutaraldehyde a defined mixture of allergens derived from Phleum pratense grass pollen and native mannan (non-oxidized) from Saccharomyces cerevisae. Analytical and structural studies, including 2D-DOSY and (1)H-(13)C HSQC nuclear magnetic resonance spectra, demonstrated the feasibility of such an approach. The glycoconjugates obtained were polymers of high molecular weight showing a higher stability than the native allergen or the conventional allergoid without mannan. The allergoid-mannan glycoconjugates were hypoallergenic as detected by the IgE reactivity with sera from grass allergic patients, even with lower reactivity than conventional allergoid without mannan. Thus, stable hypoallergenic allergoids conjugated to mannan suitable for using in immunotherapy can be achieved using glutaraldehyde. In contrast to mannan oxidation, the glutaraldehyde approach allows to preserve mannoses with their native geometry, which may be functionally important for its receptor-mediated recognition.
authors.affiliation: Centro de Investigaciones Biológicas, Madrid, Spain.
authors.forename: Ana I
authors.initials: AI
authors.lastname: Manzano
authors.affiliation: Centro de Investigaciones Biológicas, Madrid, Spain.
authors.forename: F
authors.initials: F
authors.lastname: Javier Cañada
authors.affiliation: Inmunotek SL, Punto Mobi 5, Alcalá de Henares, Madrid, Spain.
authors.forename: Bárbara
authors.initials: B
authors.lastname: Cases
authors.affiliation: Facultad de Químicas, Universidad Complutense, Madrid, Spain.
authors.forename: Sofia
authors.initials: S
authors.lastname: Sirvent
authors.affiliation: Inmunotek SL, Punto Mobi 5, Alcalá de Henares, Madrid, Spain.
authors.forename: Irene
authors.initials: I
authors.lastname: Soria
authors.affiliation: Facultad de Químicas, Universidad Complutense, Madrid, Spain.
authors.forename: Oscar
authors.initials: O
authors.lastname: Palomares
authors.affiliation: Inmunotek SL, Punto Mobi 5, Alcalá de Henares, Madrid, Spain.
authors.forename: Enrique
authors.initials: E
authors.lastname: Fernández-Caldas
authors.affiliation: Inmunotek SL, Punto Mobi 5, Alcalá de Henares, Madrid, Spain.
authors.forename: Miguel
authors.initials: M
authors.lastname: Casanovas
authors.affiliation: Centro de Investigaciones Biológicas, Madrid, Spain.
authors.forename: Jesús
authors.initials: J
authors.lastname: Jiménez-Barbero
authors.affiliation: Inmunotek SL, Punto Mobi 5, Alcalá de Henares, Madrid, Spain. jlsubiza@inmunotek.com.
authors.forename: José L
authors.initials: JL
authors.lastname: Subiza
mesh_terms: D000485:Allergens / Q000737:chemistry* / Q000276:immunology; D000937:Antigen-Antibody Reactions; D062610:Fungal Polysaccharides / Q000737:chemistry* / Q000276:immunology; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology; D006109:Poaceae; D011058:Pollen / Q000737:chemistry* / Q000276:immunology; D012441:Saccharomyces cerevisiae / Q000737:chemistry; D014612:Vaccines / Q000276:immunology
chemical_list: D000485:Allergens; D062610:Fungal Polysaccharides; D014612:Vaccines; D007073:Immunoglobulin E
keywords: Allergen; Allergoid; Conjugation; Glutaraldehyde; Mannan; Vaccine