title: Dual Action of Lysophosphatidate-Functionalised Titanium: Interactions with Human (MG63) Osteoblasts and Methicillin Resistant Staphylococcus aureus.
abstract: Titanium (Ti) is a widely used material for surgical implants; total joint replacements (TJRs), screws and plates for fixing bones and dental implants are forged from Ti. Whilst Ti integrates well into host tissue approximately 10% of TJRs will fail in the lifetime of the patient through a process known as aseptic loosening. These failures necessitate revision arthroplasties which are more complicated and costly than the initial procedure. Finding ways of enhancing early (osseo)integration of TJRs is therefore highly desirable and continues to represent a research priority in current biomaterial design. One way of realising improvements in implant quality is to coat the Ti surface with small biological agents known to support human osteoblast formation and maturation at Ti surfaces. Lysophosphatidic acid (LPA) and certain LPA analogues offer potential solutions as Ti coatings in reducing aseptic loosening. Herein we present evidence for the successful bio-functionalisation of Ti using LPA. This modified Ti surface heightened the maturation of human osteoblasts, as supported by increased expression of alkaline phosphatase. These functionalised surfaces also deterred the attachment and growth of Staphylococcus aureus, a bacterium often associated with implant failures through sepsis. Collectively we provide evidence for the fabrication of a dual-action Ti surface finish, a highly desirable feature towards the development of next-generation implantable devices.
authors.affiliation: Department of Systems Biology, Technical University of Denmark, Kgs. Lyngby, Denmark.|Department for Infection and Microbiology Control, Statens Serum Institut, Copenhagen S, Denmark.
authors.forename: Mette Elena
authors.initials: ME
authors.lastname: Skindersoe
authors.affiliation: Department for Infection and Microbiology Control, Statens Serum Institut, Copenhagen S, Denmark.
authors.forename: Karen A
authors.initials: KA
authors.lastname: Krogfelt
authors.affiliation: Musculoskeletal Research Unit, University of Bristol, Southmead Hospital, Bristol, BS10 5NB, United Kingdom.
authors.forename: Ashley
authors.initials: A
authors.lastname: Blom
authors.affiliation: Department of Medicinal Chemistry, The University of Utah, Salt Lake City, Utah, United States of America.
authors.forename: Jianxing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Medicinal Chemistry, The University of Utah, 419 Wakara Way, Suite 205, Salt Lake City, Utah 84108, United States of America.
authors.forename: Guowei
authors.initials: G
authors.lastname: Jiang
authors.affiliation: Department of Medicinal Chemistry, The University of Utah, 419 Wakara Way, Suite 205, Salt Lake City, Utah 84108, United States of America.
authors.forename: Glenn D
authors.initials: GD
authors.lastname: Prestwich
authors.affiliation: Department of Biological, Biomedical & Analytical Sciences, University of the West of England, Frenchay Campus, Bristol, BS16 1QY, United Kingdom.
authors.forename: Jason Peter
authors.initials: JP
authors.lastname: Mansell
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D001422:Bacterial Adhesion / Q000187:drug effects; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D002460:Cell Line; D006801:Humans; D008246:Lysophospholipids / Q000737:chemistry / Q000494:pharmacology; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism* / Q000648:ultrastructure; D010006:Osteoblasts / Q000378:metabolism*; D014025:Titanium / Q000737:chemistry / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D008246:Lysophospholipids; D014025:Titanium; C032881:lysophosphatidic acid