title: Fucosyltransferase 8 expression in breast cancer patients: A high throughput tissue microarray analysis.
abstract: The aim of this study was to compare the expression of fucosyltransferase 8 (FUT8) in breast cancer tissue and to investigate the relationship between this marker with tumor progression and its applicability to differential diagnosis. An immunohistochemical study was performed for FUT8 using the tissue microarray technique. In addition, the mRNA and protein levels of FUT8 in the tissue were also tested by real-time PCR and Western blot. There was a significant difference in cytoplasmic expression of FUT8 between breast cancer tissue and matched normal tissue (p<0.001). The percent of FUT8 staining in breast cancer tissues ranging from negative, weak positive, positive and strong positive were 2.7%, 40.2%, 54% and 3.2%, respectively. High FUT8 protein expression correlated with lymphatic metastasis (p=0.008) and with stage status (p=0.039). We detected that reduced FUT8 expression correlated with disease-free survival (p=0.02) and overall survival (p=0.04) of breast cancer patients. Expression of FUT8 can stratify breast cancer tissue and may be considered a prognostic marker for breast cancer patients.
authors.affiliation: College of Pharmacy, Harbin Medical University, Harbin, and Research Institute of Medicine and Pharmacy, Qiqihar Medical University, Qiqihar, P.R. China.
authors.forename: Liling
authors.initials: L
authors.lastname: Yue
authors.affiliation: College of Pharmacy, Harbin Medical University, Harbin, P.R. China.
authors.forename: Cuicui
authors.initials: C
authors.lastname: Han
authors.affiliation: Breast Surgery, the Third Affiliated Hospital of Qiqihaer Medical University, Qiqihar, P.R. China.
authors.forename: Zubin
authors.initials: Z
authors.lastname: Li
authors.affiliation: Breast Surgery, the Third Affiliated Hospital of Qiqihaer Medical University, Qiqihar, P.R. China.
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.affiliation: Research Institute of Medicine and Pharmacy, Qiqihar Medical University, Qiqihar, P.R. China.
authors.forename: Deshui
authors.initials: D
authors.lastname: Liu
authors.affiliation: College of Pharmacy, Harbin Medical University, Harbin, P.R. China.
authors.forename: Shulin
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Biology Genetics, Qiqihar Medical University, Qiqihar, P.R. China. yuhtyuhtyuht@126.com.
authors.forename: Haitao
authors.initials: H
authors.lastname: Yu
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000032:analysis*; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000201:enzymology* / Q000401:mortality / Q000473:pathology; D018270:Carcinoma, Ductal, Breast / Q000201:enzymology* / Q000401:mortality / Q000473:pathology; D018572:Disease-Free Survival; D005260:Female; D005647:Fucosyltransferases / Q000032:analysis / Q000096:biosynthesis*; D057166:High-Throughput Screening Assays; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008875:Middle Aged; D011379:Prognosis; D060888:Real-Time Polymerase Chain Reaction; D046888:Tissue Array Analysis
chemical_list: D014408:Biomarkers, Tumor; D005647:Fucosyltransferases; C020559:Glycoprotein 6-alpha-L-fucosyltransferase