title: Effects of Liposomal Compositions with Oxidized Dextrans on Functional Activity of U937 Macrophage-Like Cells In Vitro.
abstract: We studied the effects of liposomal pharmaceutical compositions with oxidized dextrans on functional activity of U937 monocyte/macrophage-like cells. Liposomes in the emulsion contained oxidized dextran with a molecular weights of 40 kDa or 70 kDa or isonicotinic acid hydrazide (INAH) conjugated with oxidized dextran (40 kDa). Cell viability was evaluated by MTT test; mitochondrial transmembrane potential and production of superoxide anion and H2O2 were studied by fluorescent methods. The studied compositions exhibited no cytotoxic effect and even improved cell viability and mitochondrial respiration. Liposomes with oxidized 40 kDa dextran, including those with INAH-conjugated dextran, inhibited production of superoxide anion, but increased H2O2 generation.
authors.affiliation: Research Institute of Experimental and Clinical Medicine, Novosibirsk, Russia.
authors.forename: P M
authors.initials: PM
authors.lastname: Kozhin
authors.affiliation: Research Institute of Experimental and Clinical Medicine, Novosibirsk, Russia.
authors.forename: A V
authors.initials: AV
authors.lastname: Chechushkov
authors.affiliation: Research Institute of Experimental and Clinical Medicine, Novosibirsk, Russia.
authors.forename: N S
authors.initials: NS
authors.lastname: Zaitseva
authors.affiliation: Research Institute of Experimental and Clinical Medicine, Novosibirsk, Russia.
authors.forename: A E
authors.initials: AE
authors.lastname: Lemza
authors.affiliation: Research Institute of Experimental and Clinical Medicine, Novosibirsk, Russia. lemen@centercem.ru.
authors.forename: E B
authors.initials: EB
authors.lastname: Men'shchikova
authors.affiliation: Research Institute of Experimental and Clinical Medicine, Novosibirsk, Russia.
authors.forename: A V
authors.initials: AV
authors.lastname: Troitskii
authors.affiliation: Research Institute of Experimental and Clinical Medicine, Novosibirsk, Russia.|Novosibirsk State Medical University, Ministry of Health of the Russian Federation, Novosibirsk, Russia.
authors.forename: V A
authors.initials: VA
authors.lastname: Shkurupy
mesh_terms: D000995:Antitubercular Agents / Q000494:pharmacology*; D002470:Cell Survival; D003911:Dextrans / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004655:Emulsions; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D007538:Isoniazid / Q000494:pharmacology*; D008081:Liposomes; D008264:Macrophages / Q000187:drug effects*; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects; D008970:Molecular Weight; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects; D010713:Phosphatidylcholines; D013481:Superoxides / Q000378:metabolism; D020298:U937 Cells
chemical_list: D000995:Antitubercular Agents; D003911:Dextrans; D004655:Emulsions; D008081:Liposomes; D010713:Phosphatidylcholines; D013481:Superoxides; D006861:Hydrogen Peroxide; D007538:Isoniazid
keywords: isonicotinic acid hydrazide; macrophages; oxidized dextran; reactive oxygen species; viability