title: Abo1, a conserved bromodomain AAA-ATPase, maintains global nucleosome occupancy and organisation.
abstract: Maintenance of the correct level and organisation of nucleosomes is crucial for genome function. Here, we uncover a role for a conserved bromodomain AAA-ATPase, Abo1, in the maintenance of nucleosome architecture in fission yeast. Cells lacking abo1(+) experience both a reduction and mis-positioning of nucleosomes at transcribed sequences in addition to increased intragenic transcription, phenotypes that are hallmarks of defective chromatin re-establishment behind RNA polymerase II. Abo1 is recruited to gene sequences and associates with histone H3 and the histone chaperone FACT. Furthermore, the distribution of Abo1 on chromatin is disturbed by impaired FACT function. The role of Abo1 extends to some promoters and also to silent heterochromatin. Abo1 is recruited to pericentromeric heterochromatin independently of the HP1 ortholog, Swi6, where it enforces proper nucleosome occupancy. Consequently, loss of Abo1 alleviates silencing and causes elevated chromosome mis-segregation. We suggest that Abo1 provides a histone chaperone function that maintains nucleosome architecture genome-wide.
authors.affiliation: Institute for Cell & Molecular Biosciences, Newcastle University, Newcastle, UK.
authors.forename: Csenge
authors.initials: C
authors.lastname: Gal
authors.affiliation: Institute for Cell & Molecular Biosciences, Newcastle University, Newcastle, UK.
authors.forename: Heather E
authors.initials: HE
authors.lastname: Murton
authors.affiliation: Wellcome Trust Centre for Cell Biology & Institute of Cell Biology, University of Edinburgh, Edinburgh, UK.
authors.forename: Lakxmi
authors.initials: L
authors.lastname: Subramanian
authors.affiliation: Institute for Cell & Molecular Biosciences, Newcastle University, Newcastle, UK.
authors.forename: Alex J
authors.initials: AJ
authors.lastname: Whale
authors.affiliation: Biosciences, College of Life & Environmental Sciences, University of Exeter, Exeter, UK.
authors.forename: Karen M
authors.initials: KM
authors.lastname: Moore
authors.affiliation: Biosciences, College of Life & Environmental Sciences, University of Exeter, Exeter, UK.
authors.forename: Konrad
authors.initials: K
authors.lastname: Paszkiewicz
authors.affiliation: Department of Genetics, Evolution & Environment and UCL Cancer Institute, University College London, London, UK.
authors.forename: Sandra
authors.initials: S
authors.lastname: Codlin
authors.affiliation: Department of Genetics, Evolution & Environment and UCL Cancer Institute, University College London, London, UK.
authors.forename: Jürg
authors.initials: J
authors.lastname: Bähler
authors.affiliation: Department of Pathology, St. Jude Children's Research Hospital, Memphis, TN, USA.
authors.forename: Kevin M
authors.initials: KM
authors.lastname: Creamer
authors.affiliation: Department of Pathology, St. Jude Children's Research Hospital, Memphis, TN, USA.
authors.forename: Janet F
authors.initials: JF
authors.lastname: Partridge
authors.affiliation: Wellcome Trust Centre for Cell Biology & Institute of Cell Biology, University of Edinburgh, Edinburgh, UK.
authors.forename: Robin C
authors.initials: RC
authors.lastname: Allshire
authors.affiliation: Cardiff School of Biosciences, Cardiff University, Cardiff, UK kentn@cardiff.ac.uk simon.whitehall@ncl.ac.uk.
authors.forename: Nicholas A
authors.initials: NA
authors.lastname: Kent
authors.affiliation: Institute for Cell & Molecular Biosciences, Newcastle University, Newcastle, UK kentn@cardiff.ac.uk simon.whitehall@ncl.ac.uk.
authors.forename: Simon K
authors.initials: SK
authors.lastname: Whitehall
mesh_terms: D000251:Adenosine Triphosphatases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002843:Chromatin / Q000235:genetics* / Q000378:metabolism*; D042002:Chromatin Assembly and Disassembly; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism; D020090:Chromosome Segregation; D021901:DNA, Intergenic; D020868:Gene Silencing; D056488:Histone Chaperones / Q000235:genetics / Q000378:metabolism; D006657:Histones / Q000235:genetics / Q000378:metabolism; D009707:Nucleosomes / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D012319:RNA Polymerase II / Q000235:genetics; D012568:Schizosaccharomyces / Q000235:genetics* / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D021901:DNA, Intergenic; D056488:Histone Chaperones; D006657:Histones; D009707:Nucleosomes; D029702:Schizosaccharomyces pombe Proteins; D014157:Transcription Factors; D012319:RNA Polymerase II; D000251:Adenosine Triphosphatases
keywords: Abo1; Schizosaccharomyces pombe; bromodomain AAA‐ATPases; chromatin; nucleosome mapping