title: Retrospective testing and case series study of porcine delta coronavirus in U.S. swine herds.
abstract: Porcine deltacoronavirus (PDCoV) was first reported in the United States (US) in February 2014. This was the second novel swine enteric coronavirus detected in the US since May 2013. In this study, we conducted retrospective testing of samples submitted to three veterinary diagnostic laboratories where qualifying biological samples were derived from previously submitted diagnostic case submissions from US commercial swine farms with a clinical history of enteric disease or from cases that had been previously tested for transmissible gastroenteritis virus, PEDV, or rotavirus. Overall, 2286 banked samples were tested from 27 States. Samples were collected in 3 separate years and in 17 different months. Test results revealed 4 positive samples, 3 collected in August 2013 and 1 collected in October 2013. In addition, a case series including 42 operations in 10 States was conducted through administration of a survey. Survey data collected included information on characteristics of swine operations that had experienced PDCoV clinical signs. Special emphasis was placed on obtaining descriptive estimates of biosecurity practices and disease status over time of each operation. Clinical signs of PDCoV were reported to be similar to those of PEDV. The average number of animals on each operation exhibiting clinical signs (morbidity) and the average number of case fatalities was greatest for suckling and weaned pigs. Average operation-level weaned pig morbidity was greatest in the first week of the outbreak while average operation-level suckling pig case fatality was greatest in the second week of the outbreak. The survey included questions regarding biosecurity practices for visitors and operation employees; trucks, equipment and drivers; and feed sources. These questions attempted to identify a likely pathway of introduction of PDCoV onto the operations surveyed.
authors.affiliation: USDA, Animal and Plant Health Inspection Service, Veterinary Services, United States. Electronic address: brian.j.mccluskey@aphis.usda.gov.
authors.forename: Brian J
authors.initials: BJ
authors.lastname: McCluskey
authors.affiliation: USDA, Animal and Plant Health Inspection Service, Veterinary Services, United States.
authors.forename: Charles
authors.initials: C
authors.lastname: Haley
authors.affiliation: Veterinary Diagnostic Laboratory, College of Veterinary Medicine, University of Minnesota, United States.
authors.forename: Albert
authors.initials: A
authors.lastname: Rovira
authors.affiliation: Veterinary Diagnostic Laboratory, College of Veterinary Medicine, Iowa State University, United States.
authors.forename: Rodger
authors.initials: R
authors.lastname: Main
authors.affiliation: Ohio Department of Agriculture, Animal Disease Diagnostic Laboratory, United States.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Veterinary Diagnostic Laboratory, College of Veterinary Medicine, University of Minnesota, United States.
authors.forename: Sunny
authors.initials: S
authors.lastname: Barder
mesh_terms: D000818:Animals; D018352:Coronavirus Infections / Q000453:epidemiology / Q000401:mortality / Q000662:veterinary* / Q000821:virology; D003967:Diarrhea / Q000453:epidemiology / Q000401:mortality / Q000662:veterinary* / Q000821:virology; D009017:Morbidity; D053485:Porcine epidemic diarrhea virus / Q000302:isolation & purification*; D015995:Prevalence; D012189:Retrospective Studies; D012307:Risk Factors; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology* / Q000401:mortality / Q000821:virology; D014481:United States / Q000453:epidemiology
keywords: Case series; Porcine deltacoronavirus; Swine enteric coronaviruses