title: Increased Contractile Response to Noradrenaline Induced By Factors Associated with the Metabolic Syndrome in Cultured Small Mesenteric Arteries.
abstract: This study investigated the effect of the metabolic syndrome associated risk factors hyperglycemia (glucose [Glc]), hyperinsulinemia (insulin [Ins]) and low-grade inflammation (tumor necrosis factor α [TNFα]) on the vasomotor responses of resistance arteries. Isolated small mesenteric arteries from 3-month-old Sprague-Dawley rats, were suspended for 21-23 h in tissue cultures containing either elevated Glc (30 mmol/l), Ins (100 nmol/l), TNFα (100 ng/ml) or combinations thereof. After incubation, the vascular response to noradrenaline (NA), phenylephrine, isoprenaline and NA in the presence of propranolol (10 µmol/l) was measured by wire myography.
  
  
  RESULTS
  Arteries exposed only to combinations of the risk factors showed a significant 1.6-fold increase in the contractile NA sensitivity, which suggests that complex combinations of metabolic risk factors might lead to changes in vascular tone.
authors.affiliation: Diabetes Biology, Novo Nordisk A/S, Novo Nordisk Park, Mx00E5;lx00F8;v, Denmark.
authors.forename: Martin
authors.initials: M
authors.lastname: Blædel
authors.forename: Anette
authors.initials: A
authors.lastname: Sams
authors.forename: Harrie C M
authors.initials: HC
authors.lastname: Boonen
authors.forename: Majid
authors.initials: M
authors.lastname: Sheykhzade
mesh_terms: D000818:Animals; D005947:Glucose / Q000494:pharmacology*; D007328:Insulin / Q000494:pharmacology*; D007545:Isoproterenol / Q000494:pharmacology; D008297:Male; D008638:Mesenteric Arteries / Q000187:drug effects*; D024821:Metabolic Syndrome / Q000503:physiopathology; D009119:Muscle Contraction / Q000187:drug effects*; D009213:Myography; D009638:Norepinephrine / Q000494:pharmacology*; D010656:Phenylephrine / Q000494:pharmacology; D011433:Propranolol / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
chemical_list: D007328:Insulin; D014409:Tumor Necrosis Factor-alpha; D010656:Phenylephrine; D011433:Propranolol; D005947:Glucose; D007545:Isoproterenol; D009638:Norepinephrine