title: Characterization of magnetic nanoparticles from Magnetospirillum Gryphiswaldense as potential theranostics tools.
abstract: We investigated the theranostic properties of magnetosomes (MNs) extracted from magnetotactic bacteria, promising for nanomedicine applications. Besides a physico-chemical characterization, their potentiality as mediators for magnetic fluid hyperthermia and contrast agents for magnetic resonance imaging, both in vitro and in vivo, are here singled out. The MNs, constituted by magnetite nanocrystals arranged in chains, show a superparamagnetic behaviour and a clear evidence of Verwey transition, as signature of magnetite presence. The phospholipid membrane provides a good protection against oxidation and the MNs oxidation state is stable over months. Using an alternate magnetic field, the specific absorption rate was measured, resulting among the highest reported in literature. The MRI contrast efficiency was evaluated by means of the acquisition of complete NMRD profiles. The transverse relaxivity resulted as high as the one of a former commercial contrast agent. The MNs were inoculated into an animal model of tumour and their presence was detected by magnetic resonance images two weeks after the injection in the tumour mass.
authors.affiliation: Department of Physics and INSTM, Università degli Studi di Pavia, Pavia, I-27100, Italy.|Research Group EPR Spectroscopy, Max Planck Institute for Biophysical Chemistry, Göttingen, D-37077, Germany.
authors.forename: T
authors.initials: T
authors.lastname: Orlando
authors.affiliation: Department of Neurological and Movement Science and INSTM, University of Verona, Verona, I-37134, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Mannucci
authors.affiliation: Department of Chemistry, 'Ugo Schiff' University of Florence and INSTM, Sesto Fiorentino (FI), I-50019, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Fantechi
authors.affiliation: Department of Neurological and Movement Science and INSTM, University of Verona, Verona, I-37134, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Conti
authors.affiliation: Department of Neurological and Movement Science and INSTM, University of Verona, Verona, I-37134, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Tambalo
authors.affiliation: Department of Neurological and Movement Science and INSTM, University of Verona, Verona, I-37134, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Busato
authors.affiliation: Department of Chemistry, 'Ugo Schiff' University of Florence and INSTM, Sesto Fiorentino (FI), I-50019, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Innocenti
authors.affiliation: Department of Biotechnology and INSTM, University of Verona, Verona, I-37134, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Ghin
authors.affiliation: Department of Biotechnology and INSTM, University of Verona, Verona, I-37134, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Bassi
authors.affiliation: Department of Physics and INSTM, Università degli Studi di Milano, Milano, I-20133, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Arosio
authors.affiliation: Department of Physics and INSTM, Università degli Studi di Milano, Milano, I-20133, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Orsini
authors.affiliation: CNR-ICCOM and INSTM, Sesto Fiorentino (FI), I-50019, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Sangregorio
authors.affiliation: Department of Physics and INSTM, Università degli Studi di Pavia, Pavia, I-27100, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Corti
authors.affiliation: Department of Chemical and Geological Science and INSTM, University of Cagliari, Monserrato (CA), I-09042, Italy.
authors.forename: M F
authors.initials: MF
authors.lastname: Casula
authors.affiliation: Department of Computer Science and INSTM, University of Verona, Verona, I-37134, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Marzola
authors.affiliation: Department of Physics and INSTM, Università degli Studi di Milano, Milano, I-20133, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Lascialfari
authors.affiliation: Department of Neurological and Movement Science and INSTM, University of Verona, Verona, I-37134, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Sbarbati
mesh_terms: D000818:Animals; D003287:Contrast Media / Q000008:administration & dosage* / Q000737:chemistry; D004195:Disease Models, Animal; D006801:Humans; D008279:Magnetic Resonance Imaging; D058185:Magnetite Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D055944:Magnetosomes; D042201:Magnetospirillum / Q000737:chemistry; D051379:Mice; D009369:Neoplasms / Q000000981:diagnostic imaging* / Q000473:pathology; D000068936:Theranostic Nanomedicine; D023041:Xenograft Model Antitumor Assays
chemical_list: D003287:Contrast Media; D058185:Magnetite Nanoparticles
keywords: MRI, hyperthermia; contrast agent; magnetosomes; relaxometry; theranostics