title: Persistent Simian Immunodeficiency Virus Infection Drives Differentiation, Aberrant Accumulation, and Latent Infection of Germinal Center Follicular T Helper Cells.
abstract: CD4(+) follicular T helper (Tfh) cells play a prominent role in humoral immune responses, but the mechanisms of their accumulation and infection in AIDS remain unclear. Here we found that germinal center (GC) Tfh cells, defined here as CXCR5(+) PD-1(HIGH) CD4(+) T cells, do not express the HIV coreceptor CCR5 yet serve as a latent reservoir in GCs. With disease progression, an expansion of GC Tfh cells is accompanied by increases in dysfunctional CD8(+) T cells. In contrast, Tfh precursor (CXCR5(-) CD4(+) T) cells in lymph nodes do express CCR5 and differentiate into GC Tfh cells following interleukin-6 (IL-6) and IL-21 stimulation, and viral DNA is detectable in fully differentiated GC Tfh cells ex vivo. This suggests that SIV-infected GC Tfh cells may be derived from Tfh precursor cell subsets that become infected in marginal zones and then migrate into GCs as fully mature GC Tfh cells that serve as persistent virus reservoirs. These findings suggest that viral persistence in lymph nodes drives compensatory differentiation, aberrant accumulation, and latent infection of GC Tfh cells, resulting in marked impairment of humoral immune responses.
  
  
  OBJECTIVE
  Generation of antibodies that can effectively eliminate viruses requires interactions of B cells with highly specialized T cells in GCs of lymphoid tissues called follicular T helper cells. Here we show that in simian immunodeficiency virus infection, these cells are initially infected in a precursor stage that leads to alterations in their homing, accumulation, and function that may be responsible for the inability of human immunodeficiency virus-infected patients to generate effective antibody responses.
authors.affiliation: Tulane National Primate Research Center, Tulane University School of Medicine, Covington, Louisiana, USA.
authors.forename: Huanbin
authors.initials: H
authors.lastname: Xu
authors.affiliation: Tulane National Primate Research Center, Tulane University School of Medicine, Covington, Louisiana, USA.
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Wang
authors.affiliation: Tulane National Primate Research Center, Tulane University School of Medicine, Covington, Louisiana, USA.
authors.forename: Naomi
authors.initials: N
authors.lastname: Malam
authors.affiliation: Tulane National Primate Research Center, Tulane University School of Medicine, Covington, Louisiana, USA.
authors.forename: Pyone P
authors.initials: PP
authors.lastname: Aye
authors.affiliation: Tulane National Primate Research Center, Tulane University School of Medicine, Covington, Louisiana, USA.
authors.forename: Xavier
authors.initials: X
authors.lastname: Alvarez
authors.affiliation: Tulane National Primate Research Center, Tulane University School of Medicine, Covington, Louisiana, USA.
authors.forename: Andrew A
authors.initials: AA
authors.lastname: Lackner
authors.affiliation: Tulane National Primate Research Center, Tulane University School of Medicine, Covington, Louisiana, USA rveazey@tulane.edu.
authors.forename: Ronald S
authors.initials: RS
authors.lastname: Veazey
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000737:chemistry / Q000821:virology*; D002454:Cell Differentiation; D018858:Germinal Center / Q000821:virology*; D016130:Immunophenotyping; D008253:Macaca mulatta; D061026:Programmed Cell Death 1 Receptor / Q000032:analysis; D054380:Receptors, CXCR5 / Q000032:analysis; D016097:Simian Acquired Immunodeficiency Syndrome / Q000473:pathology / Q000821:virology*; D015302:Simian Immunodeficiency Virus / Q000254:growth & development / Q000502:physiology*; D016176:T-Lymphocyte Subsets / Q000737:chemistry / Q000821:virology*; D006377:T-Lymphocytes, Helper-Inducer / Q000737:chemistry / Q000821:virology*; D017735:Virus Latency
chemical_list: D061026:Programmed Cell Death 1 Receptor; D054380:Receptors, CXCR5