title: Enzyme-Operated DNA-Based Nanodevices.
abstract: Functional molecular nanodevices and nanomachines have attracted a growing interest for their potential use in life science and nanomedicine. In particular, due to their versatility and modularity DNA-based nanodevices appear extremely promising. However, a limitation of such devices is represented by the limited number of molecular stimuli and cues that can be used to control and regulate their function. Here we demonstrate the possibility to rationally control and regulate DNA-based nanodevices using biocatalytic reactions catalyzed by different enzymes. To demonstrate the versatility of our approach, we have employed three model DNA-based systems and three different enzymes (belonging to several classes, i.e., transferases and hydrolases). The possibility to use enzymes and enzymatic substrates as possible cues to operate DNA-based molecular nanodevices will expand the available toolbox of molecular stimuli to be used in the field of DNA nanotechnology and could open the door to many applications including enzyme-induced drug delivery and enzyme-triggered nanostructures assembly.
authors.affiliation: Department of Chemical Science and Technology, University of Rome Tor Vergata , 00133, Rome, Italy.
authors.forename: Erica
authors.initials: E
authors.lastname: Del Grosso
authors.affiliation: Laboratory of Biosensors and Nanomachines, Département de Chimie, Université de Montréal , Québec QC H3T 1J4, Canada.
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Dallaire
authors.affiliation: Laboratory of Biosensors and Nanomachines, Département de Chimie, Université de Montréal , Québec QC H3T 1J4, Canada.
authors.forename: Alexis
authors.initials: A
authors.lastname: Vallée-Bélisle
authors.affiliation: Department of Chemical Science and Technology, University of Rome Tor Vergata , 00133, Rome, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Ricci
mesh_terms: D055162:Biocatalysis; D004247:DNA / Q000737:chemistry*; D036103:Nanotechnology
chemical_list: D004247:DNA
keywords: DNA nanostructures; DNA nanotechnology; enzymes; molecular devices