title: Detection of Neisseria meningitidis using surface plasmon resonance based DNA biosensor.
abstract: Herein, we report the development of a surface plasmon resonance (SPR) based biosensor for the detection of Neisseria meningitidis DNA employing Kretschmann configuration. Highly c-axis oriented ZnO thin film of thickness 200nm was deposited on gold coated glass prisms by RF sputtering technique. Single stranded probe DNA was immobilized on the surface of ZnO thin film by physical adsorption method. SPR reflectance curves were recorded as a function of incident angle of He-Ne laser beam using a laboratory assembled SPR setup. The prepared biosensor exhibits a linear response towards target meningitidis DNA over the concentration range from 10 to 180 ng/μl with a high sensitivity of about 0.03°/(ng/μl) and a low limit of detection of 5 ng/μl. The SPR biosensor demonstrated high specificity and long shelf life thus, pointing towards a promising application in the field of meningitidis diagnosis.
authors.affiliation: Department of Physics and Astrophysics, University of Delhi, Delhi 110007, India.
authors.forename: Gurpreet
authors.initials: G
authors.lastname: Kaur
authors.affiliation: Department of Physics and Astrophysics, University of Delhi, Delhi 110007, India.
authors.forename: Ayushi
authors.initials: A
authors.lastname: Paliwal
authors.affiliation: Physics Department, Miranda House, University of Delhi, Delhi 110007, India.
authors.forename: Monika
authors.initials: M
authors.lastname: Tomar
authors.affiliation: Department of Physics and Astrophysics, University of Delhi, Delhi 110007, India. Electronic address: drguptavinay@gmail.com.
authors.forename: Vinay
authors.initials: V
authors.lastname: Gupta
mesh_terms: D000327:Adsorption; D015374:Biosensing Techniques / Q000379:methods*; D004277:DNA, Single-Stranded / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D006801:Humans; D008585:Meningitis, Meningococcal / Q000175:diagnosis* / Q000382:microbiology; D009345:Neisseria meningitidis / Q000302:isolation & purification* / Q000472:pathogenicity; D020349:Surface Plasmon Resonance; D015034:Zinc Oxide / Q000737:chemistry
chemical_list: D004277:DNA, Single-Stranded; D006046:Gold; D015034:Zinc Oxide
keywords: Biosensor; Neisseria meningitidis; Surface plasmon resonance; Zinc oxide