title: Tigriopus fulvus: The interlaboratory comparison of the acute toxicity test.
abstract: The paper reports the results of an interlaboratory comparison involving 11 laboratories, with the objectives of apply and validate a new standardized ecotoxicological method on marine crustacean Tigriopus fulvus. Copper was chosen as reference toxicant as indicated in the official method. The results of two independent tests performed by all the participants, demonstrated that the new method is simple, fast and easy to learn. This is confirmed even by the values of z-score index calculated for each laboratory and the relative coefficient of variation (CV) which are 6.32% after 24h, 6.56 after 48h and 35.3% after 96h, mentioned in the ISO standards for the precision of interlaboratory assays. Therefore its use could be recommended in environmental studies and monitoring.
authors.affiliation: ISPRA - Institute for Environmental Protection and Research, Via Brancati, 48, 00144 Rome, Italy. Electronic address: olga.faraponova@isprambiente.it.
authors.forename: Olga
authors.initials: O
authors.lastname: Faraponova
authors.affiliation: University of Genoa, DISTAV - Department of Earth, Environment and Life Sciences, Corso Europa, 26, 16132 Genoa, Italy. Electronic address: elisabettagiacco@yahoo.it.
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Giacco
authors.affiliation: CNR - IAMC Institute for the Coastal Marine Environment, National Research Council, Via Roma, 3, 74100 Taranto, Italy. Electronic address: francesca.biandolino@iamc.cnr.it.
authors.forename: Francesca
authors.initials: F
authors.lastname: Biandolino
authors.affiliation: CNR - IAMC Institute for the Coastal Marine Environment, National Research Council, Via Roma, 3, 74100 Taranto, Italy. Electronic address: linda.prato@iamc.cnr.it.
authors.forename: Ermelinda
authors.initials: E
authors.lastname: Prato
authors.affiliation: University of Naples Federico II, Department of Biological Sciences, Via Mezzocannone 16, 80134 Naple, Italy. Electronic address: fdelprete78@libero.it.
authors.forename: Francesco
authors.initials: F
authors.lastname: Del Prete
authors.affiliation: ARPAM - Regional Agency for Environmental Protection of the Marche Region, Via Federico II, 41, 62010 Macerata, Italy. Electronic address: alessandra.valenti@ambiente.marche.it.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Valenti
authors.affiliation: ARPAM - Regional Agency for Environmental Protection of the Marche Region, Via Federico II, 41, 62010 Macerata, Italy. Electronic address: stefania.sarcina@ambiente.marche.it.
authors.forename: Stefania
authors.initials: S
authors.lastname: Sarcina
authors.affiliation: University of Bologna, CIRSA - Interdepartmental Research Centre for Environmental Sciences, Via S. Alberto 163, 48123 Ravenna, Italy. Electronic address: andrea.pasteris@unibo.it.
authors.forename: Andrea
authors.initials: A
authors.lastname: Pasteris
authors.affiliation: University of Bologna, CIRSA - Interdepartmental Research Centre for Environmental Sciences, Via S. Alberto 163, 48123 Ravenna, Italy. Electronic address: adele.montecavalli@gmail.com.
authors.forename: Adele
authors.initials: A
authors.lastname: Montecavalli
authors.affiliation: ARPAV - Regional Agency for Environmental Protection of the Veneto Region, Via Lissa, 6, 30174 Venezia Mestre, Italy. Electronic address: scomin@arpa.veneto.it.
authors.forename: Stefano
authors.initials: S
authors.lastname: Comin
authors.affiliation: ARPAV - Regional Agency for Environmental Protection of the Veneto Region, Via Lissa, 6, 30174 Venezia Mestre, Italy.
authors.forename: Claudia
authors.initials: C
authors.lastname: Cesca
authors.affiliation: Shoreline Soc.Coop, Padriciano, 99, 34149 Trieste Italy. Electronic address: marco.francese@shoreline.it.
authors.forename: Marco
authors.initials: M
authors.lastname: Francese
authors.affiliation: Shoreline Soc.Coop, Padriciano, 99, 34149 Trieste Italy.
authors.forename: Monica
authors.initials: M
authors.lastname: Cigar
authors.affiliation: CNR-ISMAR-Istitute of Marine Sciences - Genova, Via De Marini, 6, 16149 Genova, Italy. Electronic address: veronica.piazza@gismar.cnr.it.
authors.forename: Veronica
authors.initials: V
authors.lastname: Piazza
authors.affiliation: ISS - Istituto Superiore di Sanità, Department of Environment and Primary Prevent, Unit of Soil and Waste, Ecotoxicology Laboratory, Viale Regina Elena, 299, 00161 Rome, Italy. Electronic address: fabrizio.falleni@iss.it.
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Falleni
authors.affiliation: ISS - Istituto Superiore di Sanità, Department of Environment and Primary Prevent, Unit of Soil and Waste, Ecotoxicology Laboratory, Viale Regina Elena, 299, 00161 Rome, Italy. Electronic address: ines.lacchetti@iss.it.
authors.forename: Ines
authors.initials: I
authors.lastname: Lacchetti
mesh_terms: D000818:Animals; D033342:Copepoda / Q000187:drug effects; D003300:Copper / Q000633:toxicity; D054750:Ecotoxicology / Q000592:standards; D005260:Female; D007753:Laboratories / Q000706:statistics & numerical data; D007814:Larva; D023382:Toxicity Tests, Acute / Q000592:standards* / Q000706:statistics & numerical data
chemical_list: D003300:Copper
keywords: Assigned value; Interlaboratory comparisons; Reference toxicant; Synchronised nauplii