title: Development and in vivo testing of a high frequency endoscopic Raman spectroscopy system for potential applications in the detection of early colonic neoplasia.
abstract: The objective of this study was to build and test an adjunct system to a colonoscope for in vivo measurement of Raman spectra from colon tissue for potentially improving the detection of early cancers. The novelty of this system was that low cost fibre optic probes were used, without the addition of expensive optical filters. Good quality in vivo Raman spectra were successfully obtained with a 1 s integration time in the high frequency (HF) range from normal tissue and polyps of patients during a colonoscopy. The polyps were subsequently removed, and their pathology determined. The acquired in vivo Raman spectra showed clear changes between tissue with normal and tubular adenoma pathology. Further clinical study with this low cost HF Raman probe is warranted to fully test its clinical utility.
authors.affiliation: Imaging Unit - Integrative Oncology Department, BC Cancer Agency Research Centre, 675 West 10th Avenue, Vancouver, B.C., Canada, V5Z 1L3.
authors.forename: Michael A
authors.initials: MA
authors.lastname: Short
authors.affiliation: Imaging Unit - Integrative Oncology Department, BC Cancer Agency Research Centre, 675 West 10th Avenue, Vancouver, B.C., Canada, V5Z 1L3.
authors.forename: Wenbo
authors.initials: W
authors.lastname: Wang
authors.affiliation: Division of Gastroenterology, University of British Columbia and Genome Sciences Centre, BC Cancer Agency Research Centre, 675 West 10th Avenue, Vancouver, B.C., Canada, V5Z 1L3.
authors.forename: Isabella T
authors.initials: IT
authors.lastname: Tai
authors.affiliation: Imaging Unit - Integrative Oncology Department, BC Cancer Agency Research Centre, 675 West 10th Avenue, Vancouver, B.C., Canada, V5Z 1L3. hzeng@bccrc.ca.
authors.forename: Haishan
authors.initials: H
authors.lastname: Zeng
mesh_terms: D003110:Colonic Neoplasms / Q000175:diagnosis*; D055088:Early Detection of Cancer / Q000295:instrumentation / Q000379:methods*; D004724:Endoscopy; D006801:Humans; D013059:Spectrum Analysis, Raman / Q000295:instrumentation / Q000379:methods*
keywords: Cancer Diagnosis; Colon; In-Vivo Raman spectroscopy; Raman probe; high frequency Raman