title: Short-term glucagon stimulation test of C-peptide effect on glucose utilization in patients with type 1 diabetes mellitus.
abstract: This work aimed to evaluate the use of a four-point glucagon stimulation test of C-peptide effect on glucose utilization in type 1 diabetic patients using a new mathematical model. A group of 32 type 1 diabetic patients and a group of 10 healthy control subjects underwent a four-point glucagon stimulation test with blood sampling at 0, 6, 15 and 30 min after 1 mg glucagon bolus intravenous administration. Pharmacokinetic and pharmacokinetic/pharmacodynamic models of C-peptide effect on glucose utilization versus area under curve (AUC) were used. A two-sample t test and ANOVA with Bonferroni correction were used to test the significance of differences between parameters. A significant difference between control and patient groups regarding the coefficient of whole-body glucose utilization and AUC C-peptide/AUC glucose ratio (p ≪ 0.001 and p = 0.002, respectively) was observed. The high correlation (r = 0.97) between modeled coefficient of whole-body glucose utilization and numerically calculated AUC C-peptide/AUC glucose ratio related to entire cohort indicated the stability of used method. The short-term four-point glucagon stimulation test allows the numerically calculated AUC C-peptide/AUC glucose ratio and/or the coefficient of whole-body glucose utilization calculated from model to be used to diagnostically identify type 1 diabetic patients.
authors.affiliation: IIIrd Clinic of Internal Medicine, Faculty of Medicine, Comenius University in Bratislava, Limbová 5, 833 05 Bratislava, Slovakia
authors.forename: Viliam
authors.initials: V
authors.lastname: Mojto
authors.affiliation: Institute of Automation, Measurement and Applied Informatics, Faculty of Mechanical Engineering, Slovak University of Technology, Namestie slobody 17, 812 31, Bratislava, Slovakia.
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Rausova
authors.affiliation: Institute of Automation, Measurement and Applied Informatics, Faculty of Mechanical Engineering, Slovak University of Technology, Namestie slobody 17, 812 31, Bratislava, Slovakia. jana.chrenova@google.com.
authors.forename: Jana
authors.initials: J
authors.lastname: Chrenova
authors.affiliation: Institute of Automation, Measurement and Applied Informatics, Faculty of Mechanical Engineering, Slovak University of Technology, Namestie slobody 17, 812 31, Bratislava, Slovakia.
authors.forename: Ladislav
authors.initials: L
authors.lastname: Dedik
mesh_terms: D000328:Adult; D001786:Blood Glucose / Q000378:metabolism*; D002096:C-Peptide / Q000378:metabolism*; D016022:Case-Control Studies; D003922:Diabetes Mellitus, Type 1 / Q000378:metabolism*; D005260:Female; D005934:Glucagon / Q000008:administration & dosage / Q000378:metabolism*; D005951:Glucose Tolerance Test; D006801:Humans; D008297:Male; D015233:Models, Statistical; D055815:Young Adult
chemical_list: D001786:Blood Glucose; D002096:C-Peptide; D005934:Glucagon
keywords: C-peptide; Glucagon stimulation test; Glucose utilization; Pharmacodynamics; Pharmacokinetics