title: Influence of heat-labile serum components in the presence of OmpA on the outer membrane of Salmonella gallinarum.
abstract: Salmonella gallinarum is the causative agent of fowl typhoid. Being a Gram-negative bacteria, its outer membrane proteins (OMP) can be regulated by different microenvironments. S. gallinarum was cultured under the following conditions: nutrient broth (NB), NB supplemented with serum from specific pathogen-free birds (NBS) and NB with serum incubated at 56 °C prior to incubation with the bacteria (NBSD); OMP were subsequently extracted. Several changes were observed in the apparent expression of OMP, mainly a decrease in an OMP with a size of 30 kDa, approximately, under the NBS condition. In contrast, the same event was not observed in NB and NBSD when using one- and two-dimensional polyacrylamide gels (SDS-PAGE). Using the OMP with a size of 30 kDa, approximately, as antigen in indirect ELISA, we were able to differentiate serum from healthy and vaccinated birds, as well as birds infected with S. gallinarum and S. enteritidis. The amino-terminal of this protein was sequenced, showing 100 % identity with OmpA of S. typhimurium. Subsequently, we designed primers to amplify the gene by PCR. The partial sequence of the amplified gene showed 100 % identity with OmpA of S. gallinarum. (1) Heat-labile serum components influence the presence of OmpA in the OM of S. gallinarum; (2) by the way of ELISA, OmpA allows to specifically differentiate healthy from diseased birds.
authors.affiliation: Facultad de Agronomía y Veterinaria, Universidad Autónoma de San Luis Potosí, San Luis Potosí, SLP, Mexico.
authors.forename: X
authors.initials: X
authors.lastname: Vega-Manriquez
authors.affiliation: Laboratorio de Microbiología Molecular, Departamento de Microbiología e Inmunología, Facultad de Medicina Veterinaria y Zootencnia, Universidad Nacional Autónoma de México, Circuito exterior S/N, Ciudad Universitaria, UNAM CU, Coyoacan México, 04510, México City, Mexico.
authors.forename: L
authors.initials: L
authors.lastname: Huerta-Ascencio
authors.affiliation: Departamento de Producción Agrícola, Universidad Autónoma Metropolitana-Unidad Xochimilco, México City, Mexico.
authors.forename: D
authors.initials: D
authors.lastname: Martínez-Gómez
authors.affiliation: Programa de Inmunología Molecular Microbiana, Facultad de Medicina, Universidad Nacional Autónoma de México, México City, Mexico.
authors.forename: Y
authors.initials: Y
authors.lastname: López-Vidal
authors.affiliation: Laboratorio de Microbiología Molecular, Departamento de Microbiología e Inmunología, Facultad de Medicina Veterinaria y Zootencnia, Universidad Nacional Autónoma de México, Circuito exterior S/N, Ciudad Universitaria, UNAM CU, Coyoacan México, 04510, México City, Mexico. antoverduro@hotmail.com.
authors.forename: A
authors.initials: A
authors.lastname: Verdugo-Rodríguez
mesh_terms: D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D002645:Chickens; D004591:Electrophoresis, Polyacrylamide Gel; D006358:Hot Temperature; D016133:Polymerase Chain Reaction; D012475:Salmonella / Q000235:genetics / Q000254:growth & development; D044967:Serum / Q000737:chemistry*
chemical_list: D001425:Bacterial Outer Membrane Proteins
keywords: ELISA; OmpA; S. gallinarum