title: Cartilage oligomeric matrix protein and matrix metalloproteinase-3 expression in the serum and joint fluid of a reversible osteoarthritis rabbit model.
abstract: The main pathological characteristic of osteoarthritis (OA) is cartilage damage. We explored cartilage oligomeric matrix protein (COMP) and matrix metalloproteinase-3 (MMP-3) changes during articular cartilage injury and repair. Rabbits were randomly divided into the following: a blank control group; groups M1, M2, and M3, in which breaking was performed for 2, 4, and 6 weeks, respectively; and groups L1, L2, and L3, in which breaking was discontinued for 2, 4, and 6 weeks, respectively, following a 4-week recovery period. There are 7 rabbits in each group. The degree of cartilage damage in each group was scored (OA score). An enzyme-linked immunosorbent assay was used to detect COMP and MMP-3 levels in serum and joint fluid. The OA scores were 3.89 ± 2.31, 7.21 ± 2.31, and 10.88 ± 2.08 points in groups M1, M2, and M3, respectively (P < 0.05). COMP and MMP-3 levels were significantly higher in groups M1, M2, and M3 than in C. The OA score improved significantly following the 4-week recovery period (P < 0.05). COMP and MMP-3 levels began to decrease as the time following discontinuation of breaking increased, but were higher than in the control (P < 0.05). MMP- 3 and COMP levels were correlated with OA score (r > 0.7, P < 0.05). COMP and MMP-3 levels were correlated between joint fluid and serum (r = 0.899, r = 0.874, P < 0.05, respectively). Long-term joint breaking can cause articular cartilage damage. Doing some activites after the process can promote self-repair of articular cartilage. COMP and MMP-3 levels were associated with articular cartilage destruction and repair.
authors.affiliation: Department of Orthopaedics, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan, China.
authors.forename: X Q
authors.initials: XQ
authors.lastname: Chu
authors.affiliation: Department of Neurosurgery, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan, China.
authors.forename: J J
authors.initials: JJ
authors.lastname: Wang
authors.affiliation: Department of Orthopaedics, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan, China.
authors.forename: L D
authors.initials: LD
authors.lastname: Dou
authors.affiliation: Department of Orthopaedics, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan, China zhaoganglife88@126.com.
authors.forename: G
authors.initials: G
authors.lastname: Zhao
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D064236:Cartilage Oligomeric Matrix Protein / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D002358:Cartilage, Articular / Q000201:enzymology / Q000378:metabolism / Q000473:pathology; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D006023:Glycoproteins / Q000097:blood / Q000378:metabolism; D008297:Male; D019278:Matrix Metalloproteinase 3 / Q000096:biosynthesis* / Q000097:blood / Q000235:genetics; D020370:Osteoarthritis, Knee / Q000097:blood / Q000235:genetics; D011817:Rabbits; D013582:Synovial Fluid / Q000201:enzymology / Q000378:metabolism*; D059467:Transcriptome
chemical_list: D015415:Biomarkers; D064236:Cartilage Oligomeric Matrix Protein; D006023:Glycoproteins; D019278:Matrix Metalloproteinase 3