title: A new genotype of border disease virus with implications for molecular diagnostics.
abstract: Border disease virus (BDV) is a (+) single-stranded RNA pestivirus affecting mainly sheep and goats worldwide. Genetic typing of BDV has led to the identification of at least seven major genotypes. This study reports the detection of a BDV strain from a goat in northwestern Italy during routine investigations. Sequence analysis revealed mutations in the 5'-UTR of the virus with implications for BDV molecular diagnostics. Moreover, subsequent phylogenetic analysis based on the combined 5'-UTR and Npro/partial C genes, showed divergence from known BDV genotypes, revealing the detection of a novel pestivirus group, for which we propose the name BDV genotype 8.
authors.affiliation: Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, via Bologna 148, 10154, Turin (TO), Italy. simone.peletto@izsto.it.
authors.forename: Simone
authors.identifier: http://orcid.org/0000-0002-8684-898X
authors.initials: S
authors.lastname: Peletto
authors.affiliation: Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, via Bologna 148, 10154, Turin (TO), Italy. claudio.caruso@izsto.it.
authors.forename: Claudio
authors.initials: C
authors.lastname: Caruso
authors.affiliation: Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, via Bologna 148, 10154, Turin (TO), Italy. francesco.cerutti@izsto.it.
authors.forename: Francesco
authors.initials: F
authors.lastname: Cerutti
authors.affiliation: Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, via Bologna 148, 10154, Turin (TO), Italy. paola.modesto@izsto.it.
authors.forename: Paola
authors.initials: P
authors.lastname: Modesto
authors.affiliation: Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, via Bologna 148, 10154, Turin (TO), Italy. simona.zoppi@izsto.it.
authors.forename: Simona
authors.initials: S
authors.lastname: Zoppi
authors.affiliation: Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, via Bologna 148, 10154, Turin (TO), Italy. alessandro.dondo@izsto.it.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Dondo
authors.affiliation: Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, via Bologna 148, 10154, Turin (TO), Italy. pierluigi.acutis@izsto.it.
authors.forename: Pier Luigi
authors.initials: PL
authors.lastname: Acutis
authors.affiliation: Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, via Bologna 148, 10154, Turin (TO), Italy. loretta.masoero@izsto.it.
authors.forename: Loretta
authors.initials: L
authors.lastname: Masoero
mesh_terms: D020121:5' Untranslated Regions; D000818:Animals; D001882:Border Disease / Q000175:diagnosis* / Q000821:virology*; D018083:Border disease virus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D016000:Cluster Analysis; D005838:Genotype; D006041:Goats; D007558:Italy; D008969:Molecular Sequence Data; D009154:Mutation; D057089:Pathology, Molecular; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D020121:5' Untranslated Regions; D012367:RNA, Viral
keywords: Border disease; Genotype; Goat; Italy; Pestivirus; Phylogenetic analysis