title: Embryonic oxidative stress results in reproductive impairment for adult zebrafish.
abstract: Exposure to environmental stressors during embryo development can have long-term effects on the adult organism. This study used the thioredoxin reductase inhibitor auranofin to investigate the consequences of oxidative stress during zebrafish development. Auranofin at low doses triggered upregulation of the antioxidant genes gstp1 and prdx1. As the dose was increased, acute developmental abnormalities, including cerebral hemorrhaging and jaw malformation, were observed. To determine whether transient disruption of redox homeostasis during development could have long-term consequences, zebrafish embryos were exposed to a low dose of auranofin from 6-24 hours post fertilization, and then raised to adulthood. The adult fish were outwardly normal in their appearance with no gross physical differences compared to the control group. However, these adult fish had reduced odds of breeding and a lower incidence of egg fertilization. This study shows that a suboptimal early life environment can reduce the chances of reproductive success in adulthood.
authors.affiliation: Department of Pathology, University of Otago, Dunedin 9016, New Zealand.
authors.forename: Trent A C
authors.initials: TAC
authors.lastname: Newman
authors.affiliation: Sir John Walsh Research Institute, University of Otago, Dunedin 9016, New Zealand.
authors.forename: Catherine R
authors.initials: CR
authors.lastname: Carleton
authors.affiliation: Sir John Walsh Research Institute, University of Otago, Dunedin 9016, New Zealand.
authors.forename: Bryony
authors.initials: B
authors.lastname: Leeke
authors.affiliation: Centre for Free Radical Research, Department of Pathology, University of Otago, Christchurch 8011, New Zealand; Maurice Wilkins Centre for Molecular Biodiscovery, New Zealand.
authors.forename: Mark B
authors.initials: MB
authors.lastname: Hampton
authors.affiliation: Department of Pathology, University of Otago, Dunedin 9016, New Zealand; Maurice Wilkins Centre for Molecular Biodiscovery, New Zealand. Electronic address: julia.horsfield@otago.ac.nz.
authors.forename: Julia A
authors.initials: JA
authors.lastname: Horsfield
mesh_terms: D000818:Animals; D001310:Auranofin / Q000633:toxicity; D004625:Embryo, Nonmammalian / Q000378:metabolism*; D047108:Embryonic Development; D005260:Female; D005298:Fertility; D008297:Male; D053858:Metabolic Networks and Pathways; D018384:Oxidative Stress; D010641:Phenotype; D012098:Reproduction; D013880:Thioredoxin-Disulfide Reductase / Q000037:antagonists & inhibitors; D015027:Zebrafish; D029961:Zebrafish Proteins / Q000037:antagonists & inhibitors
chemical_list: D029961:Zebrafish Proteins; D001310:Auranofin; D013880:Thioredoxin-Disulfide Reductase
keywords: Auranofin; Longitudinal cohort; Oxidative stress; Reproductive toxicity; Zebrafish