title: Plasma proteomic analysis of active and torpid greater mouse-eared bats (Myotis myotis).
abstract: Hibernation is a physiological adaptation to overcome extreme environmental conditions. It is characterized by prolonged periods of torpor interrupted by temporary arousals during winter. During torpor, body functions are suppressed and restored rapidly to almost pre-hibernation levels during arousal. Although molecular studies have been performed on hibernating rodents and bears, it is unclear how generalizable the results are among hibernating species with different physiology such as bats. As targeted blood proteomic analysis are lacking in small hibernators, we investigated the general plasma proteomic profile of European Myotis myotis and hibernation associated changes between torpid and active individuals by two-dimensional gel electrophoresis. Results revealed an alternation of proteins involved in transport, fuel switching, innate immunity and blood coagulation between the two physiological states. The results suggest that metabolic changes during hibernation are associated with plasma proteomic changes. Further characterization of the proteomic plasma profile identified transport proteins, coagulation proteins and complement factors and detected a high abundance of alpha-fetoprotein. We were able to establish for the first time a basic myotid bat plasma proteomic profile and further demonstrated a modulated protein expression during torpor in Myotis myotis, indicating both novel physiological pathways in bats in general, and during hibernation in particular.
authors.affiliation: Leibniz Institute for Zoo and Wildlife Research, Alfred-Kowalke-Straße 17, 10315 Berlin, Germany.
authors.forename: Alexander M
authors.initials: AM
authors.lastname: Hecht
authors.affiliation: Leibniz Institute for Zoo and Wildlife Research, Alfred-Kowalke-Straße 17, 10315 Berlin, Germany.
authors.forename: Beate C
authors.initials: BC
authors.lastname: Braun
authors.affiliation: Leibniz Institute for Molecular Pharmacology, Robert-Rössle-Straße 10, 13125 Berlin, Germany.
authors.forename: Eberhard
authors.initials: E
authors.lastname: Krause
authors.affiliation: Leibniz Institute for Zoo and Wildlife Research, Alfred-Kowalke-Straße 17, 10315 Berlin, Germany.|Department of Animal Behaviour, Freie Universität Berlin, Takustraße 3, 14195 Berlin, Germany.
authors.forename: Christian C
authors.initials: CC
authors.lastname: Voigt
authors.affiliation: Leibniz Institute for Zoo and Wildlife Research, Alfred-Kowalke-Straße 17, 10315 Berlin, Germany.|Department of Veterinary Medicine, Freie Universität Berlin, Oertzenweg 19b, 14163 Berlin, Germany.
authors.forename: Alex D
authors.initials: AD
authors.lastname: Greenwood
authors.affiliation: Leibniz Institute for Zoo and Wildlife Research, Alfred-Kowalke-Straße 17, 10315 Berlin, Germany.
authors.forename: Gábor Á
authors.initials: GÁ
authors.lastname: Czirják
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000378:metabolism*; D002685:Chiroptera / Q000097:blood / Q000378:metabolism*; D002853:Chromatography, Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D006605:Hibernation; D008297:Male; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D053719:Tandem Mass Spectrometry
chemical_list: D001798:Blood Proteins; D020543:Proteome