title: Identification and Characterization of Lineage(-)CD45(-)Sca-1(+) VSEL Phenotypic Cells Residing in Adult Mouse Bone Tissue.
abstract: Murine bone marrow (BM)-derived very small embryonic-like stem cells (BM VSELs), defined by a lineage-negative (Lin(-)), CD45-negative (CD45(-)), Sca-1-positive (Sca-1(+)) immunophenotype, were previously reported as postnatal pluripotent stem cells (SCs). We developed a highly efficient method for isolating Lin(-)CD45(-)Sca-1(+) small cells using enzymatic treatment of murine bone. We designated these cells as bone-derived VSELs (BD VSELs). The incidences of BM VSELs in the BM-derived nucleated cells and that of BD VSELs in bone-derived nucleated cells were 0.002% and 0.15%, respectively. These BD VSELs expressed a variety of hematopoietic stem cell (HSC), mesenchymal stem cell (MSC), and endothelial cell markers. The gene expression profile of the BD VSELs was clearly distinct from those of HSCs, MSCs, and ES cells. In the steady state, the BD VSELs proliferated slowly, however, the number of BD VSELs significantly increased in the bone after acute liver injury. Moreover, green fluorescent protein-mouse derived BD VSELs transplanted via tail vein injection after acute liver injury were detected in the liver parenchyma of recipient mice. Immunohistological analyses suggested that these BD VSELs might transdifferentiate into hepatocytes. This study demonstrated that the majority of the Lin(-)CD45(-)Sca-1(+) VSEL phenotypic cells reside in the bone rather than the BM. However, the immunophenotype and the gene expression profile of BD VSELs were clearly different from those of other types of SCs, including BM VSELs, MSCs, HSCs, and ES cells. Further studies will therefore be required to elucidate their cellular and/or SC characteristics and the potential relationship between BD VSELs and BM VSELs.
authors.affiliation: 1 Department of Stem Cell Biology and Regenerative Medicine, Graduate School of Medical Science, Kansai Medical University , Hirakata, Japan .
authors.forename: Ryusuke
authors.initials: R
authors.lastname: Nakatsuka
authors.affiliation: 1 Department of Stem Cell Biology and Regenerative Medicine, Graduate School of Medical Science, Kansai Medical University , Hirakata, Japan .|2 Department of Surgery, Kansai Medical University , Hirakata, Japan .
authors.forename: Ryuji
authors.initials: R
authors.lastname: Iwaki
authors.affiliation: 1 Department of Stem Cell Biology and Regenerative Medicine, Graduate School of Medical Science, Kansai Medical University , Hirakata, Japan .
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Matsuoka
authors.affiliation: 1 Department of Stem Cell Biology and Regenerative Medicine, Graduate School of Medical Science, Kansai Medical University , Hirakata, Japan .
authors.forename: Keisuke
authors.initials: K
authors.lastname: Sumide
authors.affiliation: 1 Department of Stem Cell Biology and Regenerative Medicine, Graduate School of Medical Science, Kansai Medical University , Hirakata, Japan .|3 Department of Orthopedic Surgery, Kansai Medical University , Hirakata, Japan .
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kawamura
authors.affiliation: 1 Department of Stem Cell Biology and Regenerative Medicine, Graduate School of Medical Science, Kansai Medical University , Hirakata, Japan .
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Fujioka
authors.affiliation: 1 Department of Stem Cell Biology and Regenerative Medicine, Graduate School of Medical Science, Kansai Medical University , Hirakata, Japan .
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Sasaki
authors.affiliation: 4 Division of Cancer Immunotherapy, Exploratory Oncology Research and Clinical Trial Center National Cancer Center , Chiba, Japan .
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Uemura
authors.affiliation: 5 School of Nursing, Kyoto Prefectural University of Medicine , Kyoto, Japan .
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Asano
authors.affiliation: 2 Department of Surgery, Kansai Medical University , Hirakata, Japan .
authors.forename: A-Hon
authors.initials: AH
authors.lastname: Kwon
authors.affiliation: 1 Department of Stem Cell Biology and Regenerative Medicine, Graduate School of Medical Science, Kansai Medical University , Hirakata, Japan .
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Sonoda
mesh_terms: D000375:Aging / Q000473:pathology; D000818:Animals; D000950:Antigens, Ly / Q000378:metabolism*; D001842:Bone and Bones / Q000166:cytology* / Q000378:metabolism; D019070:Cell Lineage; D002469:Cell Separation / Q000379:methods*; D048429:Cell Size; D002478:Cells, Cultured; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism; D005260:Female; D017493:Leukocyte Common Antigens / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D010641:Phenotype
chemical_list: D000950:Antigens, Ly; C074650:Ly6a protein, mouse; D008565:Membrane Proteins; D017493:Leukocyte Common Antigens; C516770:Ptprc protein, mouse