title: Isolation and molecular characterization of bovine enteroviruses in Egypt.
abstract: Enteroviruses belong to the Picornaviridae family and infect a wide range of mammals including cattle. Bovine enterovirus (BEV) has recently been reclassified into E and F serotypes. BEV was first isolated in Egypt in 1966 although it has been known in other countries since the 1950s. In this study, BEV-F2 was isolated from calves with severe diarrhea and the isolated viruses were subjected to molecular characterization. Illumina sequencing of one of the isolates revealed the presence of a complete BEV-F genome sequence. The phylogenetic analysis revealed nucleotide substitutions along the genome in comparison with other known strains of BEV-F (HQ663846, AY508697 and DQ092795). Two primer sets were designed from the 3D and 5'NTR regions and used for the examination of the remaining isolates, which were confirmed to be of the BEV-F2 serotype. The availability of the complete genome sequence of this virus adds to the sequence database of the members of Picornaviridae and should be useful in future molecular studies of BEV.
authors.affiliation: Department of Animal Medicine, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Sharkia 44511, Egypt.
authors.forename: N M
authors.initials: NM
authors.lastname: Sobhy
authors.affiliation: Department of Veterinary Population Medicine, University of Minnesota, St. Paul, MN 55108, USA.
authors.forename: S K
authors.initials: SK
authors.lastname: Mor
authors.affiliation: Department of Animal Medicine, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Sharkia 44511, Egypt.
authors.forename: M E M
authors.initials: ME
authors.lastname: Mohammed
authors.affiliation: Department of Virology, Animal Health Research Institute, Dokki, Giza 12618, Egypt.
authors.forename: I M
authors.initials: IM
authors.lastname: Bastawecy
authors.affiliation: Veterinary Serum and Vaccine Research Institute, East of Cairo 131, Egypt.
authors.forename: H M
authors.initials: HM
authors.lastname: Fakhry
authors.affiliation: Department of Microbiology, Faculty of Pharmacy, Zagazig University, Zagazig, Sharkia 44519, Egypt.
authors.forename: C R B
authors.initials: CR
authors.lastname: Youssef
authors.affiliation: Department of Animal Medicine, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Sharkia 44511, Egypt.
authors.forename: N Z
authors.initials: NZ
authors.lastname: Abouzeid
authors.affiliation: Department of Veterinary Population Medicine, University of Minnesota, St. Paul, MN 55108, USA. Electronic address: goyal001@umn.edu.
authors.forename: S M
authors.initials: SM
authors.lastname: Goyal
mesh_terms: D000818:Animals; D002417:Cattle; D003967:Diarrhea / Q000662:veterinary / Q000821:virology; D004534:Egypt; D004769:Enterovirus Infections / Q000662:veterinary* / Q000821:virology; D029650:Enterovirus, Bovine / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D005243:Feces / Q000821:virology; D016679:Genome, Viral; D010802:Phylogeny
keywords: Bovine enterovirus; Cattle; Complete sequence; Egypt; Phylogenetic analysis