title: Angiostatin prevents IL-1β-induced down-regulation of eNOS expression by inhibiting the NF-κB cascade.
abstract: This study aimed to elucidate the protective potential of angiostatin in inflamed endothelial cells in culture. We assessed the effect of angiostatin on the expression of ICAM-1 and eNOS. Angiostatin prevented IL-1β-induced down-regulation of eNOS expression, but produced no significant changes on IL-1β-induced up-regulation of ICAM-1. We then explored the effect of angiostatin on IL-1β-mediated inflammatory signaling and found that angiostatin inhibited IL-1β-mediated nuclear translocation of NF-κB. Thus, our results suggest that angiostatin prevents IL-1β-induced down-regulation of eNOS expression via inhibition of the NF-κB cascade; this may be the anti-inflammatory mechanism of angiostatin.
authors.affiliation: Department of Health Chemistry, School of Pharmacy, Iwate Medical University, Nishitokuta 2-1-1, Yahaba, Shiwa-gun, Iwate 028-3694, Japan. Electronic address: ykawasak@iwate-med.ac.jp.
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Kawasaki
authors.affiliation: Department of Health Chemistry, School of Pharmacy, Iwate Medical University, Nishitokuta 2-1-1, Yahaba, Shiwa-gun, Iwate 028-3694, Japan.
authors.forename: Eriko
authors.initials: E
authors.lastname: Yokobayashi
authors.affiliation: Department of Health Chemistry, School of Pharmacy, Iwate Medical University, Nishitokuta 2-1-1, Yahaba, Shiwa-gun, Iwate 028-3694, Japan.
authors.forename: Kentaro
authors.initials: K
authors.lastname: Sakamoto
authors.affiliation: Department of Health Chemistry, School of Pharmacy, Iwate Medical University, Nishitokuta 2-1-1, Yahaba, Shiwa-gun, Iwate 028-3694, Japan.
authors.forename: Eri
authors.initials: E
authors.lastname: Tenma
authors.affiliation: Department of Health Chemistry, School of Pharmacy, Iwate Medical University, Nishitokuta 2-1-1, Yahaba, Shiwa-gun, Iwate 028-3694, Japan.
authors.forename: Haruka
authors.initials: H
authors.lastname: Takaki
authors.affiliation: Department of Health Chemistry, School of Pharmacy, Iwate Medical University, Nishitokuta 2-1-1, Yahaba, Shiwa-gun, Iwate 028-3694, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Chiba
authors.affiliation: Department of Health Chemistry, School of Pharmacy, Iwate Medical University, Nishitokuta 2-1-1, Yahaba, Shiwa-gun, Iwate 028-3694, Japan.
authors.forename: Tomomi
authors.initials: T
authors.lastname: Otashiro
authors.affiliation: Department of Health Chemistry, School of Pharmacy, Iwate Medical University, Nishitokuta 2-1-1, Yahaba, Shiwa-gun, Iwate 028-3694, Japan.
authors.forename: Mai
authors.initials: M
authors.lastname: Ishihara
authors.affiliation: Department of Health Chemistry, School of Pharmacy, Iwate Medical University, Nishitokuta 2-1-1, Yahaba, Shiwa-gun, Iwate 028-3694, Japan.
authors.forename: Sei
authors.initials: S
authors.lastname: Yonezawa
authors.affiliation: Department of Health Chemistry, School of Pharmacy, Iwate Medical University, Nishitokuta 2-1-1, Yahaba, Shiwa-gun, Iwate 028-3694, Japan.
authors.forename: Akinori
authors.initials: A
authors.lastname: Sugiyama
authors.affiliation: Department of Health Chemistry, School of Pharmacy, Iwate Medical University, Nishitokuta 2-1-1, Yahaba, Shiwa-gun, Iwate 028-3694, Japan.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Natori
mesh_terms: D021581:Active Transport, Cell Nucleus / Q000187:drug effects; D043168:Angiostatins / Q000494:pharmacology*; D002478:Cells, Cultured; D003864:Depression, Chemical; D015536:Down-Regulation / Q000187:drug effects*; D061307:Human Umbilical Vein Endothelial Cells / Q000378:metabolism; D006801:Humans; D053583:Interleukin-1beta / Q000009:adverse effects*; D016328:NF-kappa B / Q000378:metabolism*; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D053583:Interleukin-1beta; D016328:NF-kappa B; D043168:Angiostatins; C496314:NOS3 protein, human; D052250:Nitric Oxide Synthase Type III
keywords: Angiostatin; Anti-inflammatory; Endothelial nitric oxide synthase (eNOS)