title: Development of a panel of DNA Aptamers with High Affinity for Pancreatic Ductal Adenocarcinoma.
abstract: Pancreatic cancer costs nearly 40,000 lives in the U.S. each year and has one of the lowest survival rates among cancers. Effective treatment of pancreatic ductal adenocarcinoma is hindered by lack of a reliable biomarker. To address this challenge, aptamers were selected by cell-SELEX (Systematic Evolution of Ligands by EXponential enrichment) targeting human pancreatic ductal adenocarcinoma (PL45). Five promising aptamers presenting low Kd values and good specificity were generated. Among these five aptamers, one was tailored into a nanostructure carrying a high drug payload for specific drug delivery. The results show a viability of almost 80% for negative cells while only 50% of the target cells remained alive after 48 h incubation. These results lead to the conclusion that further research could reveal protein biomarkers specific to pancreatic adenocarcinoma, with probes available for early detection.
authors.affiliation: Department of Chemistry, Department of Biochemistry and Molecular Biology, Center for Research at the Bio/Nano Interface, Health Cancer Center, UF Genetics Institute and McKnight Brain Institute, University of Florida, Gainesville, Florida, USA.
authors.forename: Carole
authors.initials: C
authors.lastname: Champanhac
authors.affiliation: Department of Chemistry, Department of Biochemistry and Molecular Biology, Center for Research at the Bio/Nano Interface, Health Cancer Center, UF Genetics Institute and McKnight Brain Institute, University of Florida, Gainesville, Florida, USA.
authors.forename: I-Ting
authors.initials: IT
authors.lastname: Teng
authors.affiliation: Department of Chemistry, Department of Biochemistry and Molecular Biology, Center for Research at the Bio/Nano Interface, Health Cancer Center, UF Genetics Institute and McKnight Brain Institute, University of Florida, Gainesville, Florida, USA.
authors.forename: Sena
authors.initials: S
authors.lastname: Cansiz
authors.affiliation: Department of Chemistry, Department of Biochemistry and Molecular Biology, Center for Research at the Bio/Nano Interface, Health Cancer Center, UF Genetics Institute and McKnight Brain Institute, University of Florida, Gainesville, Florida, USA.
authors.forename: Liqin
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Molecular Science and Biomedicine Laboratory, State Key Laboratory for Chemo/Bio Sensing and Chemometrics, College of Biology, College of Chemistry and Chemical Engineering and Collaborative Research Center of Molecular Engineering for Theranostics, Hunan University, Changsha, China.
authors.forename: Xiaoqiu
authors.initials: X
authors.lastname: Wu
authors.affiliation: Molecular Science and Biomedicine Laboratory, State Key Laboratory for Chemo/Bio Sensing and Chemometrics, College of Biology, College of Chemistry and Chemical Engineering and Collaborative Research Center of Molecular Engineering for Theranostics, Hunan University, Changsha, China.
authors.forename: Zilong
authors.initials: Z
authors.lastname: Zhoa
authors.affiliation: Molecular Science and Biomedicine Laboratory, State Key Laboratory for Chemo/Bio Sensing and Chemometrics, College of Biology, College of Chemistry and Chemical Engineering and Collaborative Research Center of Molecular Engineering for Theranostics, Hunan University, Changsha, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Fu
authors.affiliation: Department of Chemistry, Department of Biochemistry and Molecular Biology, Center for Research at the Bio/Nano Interface, Health Cancer Center, UF Genetics Institute and McKnight Brain Institute, University of Florida, Gainesville, Florida, USA.|Molecular Science and Biomedicine Laboratory, State Key Laboratory for Chemo/Bio Sensing and Chemometrics, College of Biology, College of Chemistry and Chemical Engineering and Collaborative Research Center of Molecular Engineering for Theranostics, Hunan University, Changsha, China.
authors.forename: Weihong
authors.initials: W
authors.lastname: Tan
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000737:chemistry / Q000494:pharmacology; D052157:Aptamers, Nucleotide / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D001483:Base Sequence; D021441:Carcinoma, Pancreatic Ductal / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004317:Doxorubicin / Q000737:chemistry / Q000494:pharmacology; D004337:Drug Carriers / Q000737:chemistry*; D005434:Flow Cytometry; D006801:Humans; D049329:Nanostructures / Q000737:chemistry*; D010190:Pancreatic Neoplasms / Q000378:metabolism / Q000473:pathology; D052156:SELEX Aptamer Technique
chemical_list: D000903:Antibiotics, Antineoplastic; D052157:Aptamers, Nucleotide; D004337:Drug Carriers; D004317:Doxorubicin