title: South African Herbal Extracts as Potential Chemopreventive Agents: Screening for Anticancer Splicing Activity.
abstract: RT-PCR is an invaluable tool for the detection and characterization of mRNA. Cancer cell lines are treated with crude plant extracts and RNA is extracted and purified with DNase prior to RT-PCR. RT-PCR first-strand cDNA synthesis is done using random primers and can be refrigerated at 4 °C. PCR from the stored cDNA is performed using transcript-specific primers and electrophoresed on a molecular grade agarose gel to separate the splice variants.
authors.affiliation: Research, Innovation & Engagements, Mangosuthu University of Technology, 392, Durban, 4031, South Africa. dlaminiz@mut.ac.za.
authors.forename: Zodwa
authors.initials: Z
authors.lastname: Dlamini
authors.affiliation: Mangosuthu University of Technology Research, Innovation and Engagements Portfolio, Durban, 4031, South Africa.
authors.forename: Zukile
authors.initials: Z
authors.lastname: Mbita
authors.affiliation: Faculty of Medicine and Health Sciences, Division of Pre-clinical Oncology, University of Nottingham, Nottingham, UK.
authors.forename: David O
authors.initials: DO
authors.lastname: Bates
authors.affiliation: Faculty of Medicine and Health Sciences, Division of Pre-clinical Oncology, University of Nottingham, Nottingham, UK.
authors.forename: David
authors.initials: D
authors.lastname: Bates
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D045744:Cell Line, Tumor; D018890:Chemoprevention; D004587:Electrophoresis, Agar Gel; D006801:Humans; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D016133:Polymerase Chain Reaction; D012326:RNA Splicing / Q000187:drug effects*; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification
chemical_list: D000970:Antineoplastic Agents; D010936:Plant Extracts; D012333:RNA, Messenger
keywords: Agarose gel electrophoresis; Anticancer splicing activity; DNase treatment; First-strand cDNA synthesis; Herbal plant extracts; RNA extraction