title: Characterization of thrombopoietin kinetics within 60 days after allogeneic hematopoietic stem cell transplantation and its correlation with megakaryocyte ploidy distribution.
abstract: Thrombopoietin (TPO) has been identified as a key cytokine for both megakaryogenesis and thrombopoiesis. We attempt to characterize the kinetics of TPO and its correlation with megakaryocytes (MKs) ploidy distribution pattern within 60 d after allogeneic hematopoietic stem cell transplantation (allo-HSCT). Forty-six consecutive patients undergoing allo-HSCT from October 2008 to December 2008 were included. TPO levels and ploidy distribution patterns of MKs were measured using ELISA and flow cytometric analysis, respectively. The results indicated that TPO levels and the platelet count followed opposite trends after allo-HSCT. The preconditioning TPO levels and the number of transplanted CD34(+) cells were significant predisposing factors for rapid platelet engraftment (p = 0.010 and 0.007, respectively) by multivariate analysis. There was a reduction of ploidy and an increase in immature MKs in patients with higher endogenous TPO levels (>250 pg/mL) on day 60 after allo-HSCT. Moreover, lower TPO levels (≤250 pg/mL) on day 60 after allo-HSCT were associated with significantly improved five-yr overall survival (p = 0.021) and reduced transplant-related mortality (p = 0.033). In conclusion, endogenous TPO levels may be associated with platelet recovery and have prognostic significance during allo-HSCT.
authors.affiliation: Peking University People's Hospital, Institute of Hematology, Beijing, China.
authors.forename: Haixia
authors.initials: H
authors.lastname: Fu
authors.affiliation: Peking University People's Hospital, Institute of Hematology, Beijing, China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Peking University People's Hospital, Institute of Hematology, Beijing, China.
authors.forename: Lanping
authors.initials: L
authors.lastname: Xu
authors.affiliation: Peking University People's Hospital, Institute of Hematology, Beijing, China.
authors.forename: Kaiyan
authors.initials: K
authors.lastname: Liu
authors.affiliation: Peking University People's Hospital, Institute of Hematology, Beijing, China.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Huang
mesh_terms: D000293:Adolescent; D000328:Adult; D001324:Autoantigens / Q000378:metabolism*; D001792:Blood Platelets / Q000473:pathology; D016022:Case-Control Studies; D002454:Cell Differentiation; D002648:Child; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005434:Flow Cytometry; D005500:Follow-Up Studies; D006084:Graft Rejection / Q000097:blood / Q000209:etiology*; D006085:Graft Survival; D019337:Hematologic Neoplasms / Q000628:therapy; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D007453:Iodide Peroxidase / Q000378:metabolism*; D033862:Iron-Binding Proteins / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D008533:Megakaryocytes / Q000166:cytology* / Q000378:metabolism; D008875:Middle Aged; D011003:Ploidies; D011379:Prognosis; D012307:Risk Factors; D015996:Survival Rate; D013921:Thrombocytopenia / Q000097:blood / Q000209:etiology*; D014184:Transplantation, Homologous; D055815:Young Adult
chemical_list: D001324:Autoantigens; D033862:Iron-Binding Proteins; C056658:TPO protein, human; D007453:Iodide Peroxidase
keywords: allogeneic; hematopoietic stem cell transplantation; kinetics; megakaryocyte; ploidy; thrombocytopenia; thrombopoietin