title: MiR130b-Regulation of PPARγ Coactivator- 1α Suppresses Fat Metabolism in Goat Mammary Epithelial Cells.
abstract: Fat metabolism is a complicated process regulated by a series of factors. microRNAs (miRNAs) are a class of negative regulator of proteins and play crucial roles in many biological processes; including fat metabolism. Although there have been some researches indicating that miRNAs could influence the milk fat metabolism through targeting some factors, little is known about the effect of miRNAs on goat milk fat metabolism. Here we utilized an improved miRNA detection assay, S-Poly-(T), to profile the expression of miRNAs in the goat mammary gland in different periods, and found that miR-130b was abundantly and differentially expressed in goat mammary gland. Additionally, overexpressing miR-130b impaired adipogenesis while inhibiting miR-130b enhanced adipogenesis in goat mammary epithelial cells. Utilizing 3'-UTR assay and Western Blot analusis, the protein peroxisome proliferator-activated receptor coactivator-1α (PGC1α), a major regulator of fat metabolism, was demonstrated to be a potential target of miR-130b. Interestingly, miR-130b potently repressed PGC1α expression by targeting both the PGC1α mRNA coding and 3' untranslated regions. These findings have some insight of miR-130b in mediating adipocyte differentiation by repressing PGC1α expression and this contributes to further understanding about the functional significance of miRNAs in milk fat synthesis.
authors.affiliation: Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Luo
authors.affiliation: College of Life Sciences, Shenzhen University, Shenzhen, Guangzhou, China.
authors.forename: LiuAn
authors.initials: L
authors.lastname: Ma
authors.affiliation: Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.affiliation: Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: WenTing
authors.initials: W
authors.lastname: Cao
authors.affiliation: Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: HuiFei
authors.initials: H
authors.lastname: Xu
authors.affiliation: Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: JiangJiang
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: YuTing
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.affiliation: Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: DaWei
authors.initials: D
authors.lastname: Yao
authors.affiliation: College of Medicine, Shenzhen University, Shenzhen, Guangdong, China.
authors.forename: Kang
authors.initials: K
authors.lastname: Kang
authors.affiliation: College of Life Sciences, Shenzhen University, Shenzhen, Guangzhou, China.
authors.forename: Deming
authors.initials: D
authors.lastname: Gou
mesh_terms: D050156:Adipogenesis / Q000235:genetics*; D000818:Animals; D002454:Cell Differentiation; D004847:Epithelial Cells / Q000378:metabolism*; D005260:Female; D018507:Gene Expression Regulation, Developmental; D006041:Goats / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D050356:Lipid Metabolism / Q000235:genetics*; D008321:Mammary Glands, Animal / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism; D008892:Milk / Q000378:metabolism; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: C554019:MIRN130 microRNA, human; D035683:MicroRNAs; C486497:PPARGC1A protein, human; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; D014157:Transcription Factors