title: Vitamin E analysis by ultra-performance convergence chromatography and structural elucidation of novel α-tocodienol by high-resolution mass spectrometry.
abstract: We have developed a method for analysing vitamin E using ultra-performance convergence chromatography with a chromatographic runtime of 5.5 min. A well-resolved chromatogram with excellent precision in retention time revealed seven vitamin E components in the palm oil derived tocotrienol-rich fraction. The major vitamin E components were α-tocopherol, α-tocotrienol, γ-tocotrienol and δ-tocotrienol whereas the minor vitamin E components were α-tocomonoenol, β-tocotrienol and an unreported trace component. The new component was positively identified by high-resolution mass spectrometry as 2-methyl-2(4',8',12'-trimethyltrideca-7',11'-dienyl)5,7,8-trimethylchroman-6-ol or α-tocodienol.
authors.affiliation: Palm Nutraceuticals Sdn. Bhd., Batu 7, Jalan Mawai, 81900 Kota Tinggi, Johor, Malaysia. Electronic address: geept07@yahoo.com.
authors.forename: Ping Tou
authors.initials: PT
authors.lastname: Gee
authors.affiliation: Research Instruments Sdn. Bhd., D7-1-G, Pusat Perdagangan Dana 1, Jalan PJU1A/46, 47301 Petaling Jaya, Selangor, Malaysia.
authors.forename: Chen Yee
authors.initials: CY
authors.lastname: Liew
authors.affiliation: Matrix Analytical Technologies Sdn. Bhd., 43-45B, Jalan SS 25/2, Taman Bukit Emas, 47301 Petaling Jaya, Selangor, Malaysia.
authors.forename: Meng Chil
authors.initials: MC
authors.lastname: Thong
authors.affiliation: Waters Pacific Pte Ltd., 1 Science Park Road, #02-08 The Capricorn, Singapore Science Park II, Singapore 117528.
authors.forename: Melvin C L
authors.initials: MC
authors.lastname: Gay
mesh_terms: D002839:Chromans / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D000073878:Palm Oil; D010938:Plant Oils / Q000737:chemistry; D024508:Tocotrienols / Q000032:analysis; D014810:Vitamin E / Q000031:analogs & derivatives* / Q000032:analysis* / Q000737:chemistry*; D024502:alpha-Tocopherol / Q000032:analysis
chemical_list: D002839:Chromans; D010938:Plant Oils; D024508:Tocotrienols; C000608693:alpha-tocodienol; D014810:Vitamin E; C082097:tocotrienol, delta; C013649:plastochromanol 8; D000073878:Palm Oil; C082032:tocotrienol, alpha; D024502:alpha-Tocopherol
keywords: High-resolution mass spectrometry; Tocopherol; Tocotrienol; Ultra-performance convergence chromatography; α-Tocodienol