title: Systemic administration of resveratrol suppress the nociceptive neuronal activity of spinal trigeminal nucleus caudalis in rats.
abstract: Although a modulatory role has been reported for the red wine polyphenol resveratrol on several types of ion channels and excitatory synaptic transmission in the nervous system, the acute effects of resveratrol in vivo, particularly on nociceptive transmission of the trigeminal system, remain to be determined. The aim of the present study was to investigate whether acute intravenous resveratrol administration to rats attenuates the excitability of wide dynamic range (WDR) spinal trigeminal nucleus caudalis (SpVc) neurons in response to nociceptive and non-nociceptive mechanical stimulation in vivo. Extracellular single unit recordings were made from 18 SpVc neurons in response to orofacial mechanical stimulation of pentobarbital-anesthetized rats. Responses to both non-noxious and noxious mechanical stimuli were analyzed in the present study. The mean firing frequency of SpVc WDR neurons in response to both non-noxious and noxious mechanical stimuli was inhibited by resveratrol (0.5-2 mg/kg, i.v.) and maximum inhibition of the discharge frequency of both non-noxious and noxious mechanical stimuli was seen within 10 min. These inhibitory effects were reversed after approximately 20 min. The relative magnitude of inhibition by resveratrol of SpVc WDR neuronal discharge frequency was significantly greater for noxious than non-noxious stimulation. These results suggest that, in the absence of inflammatory or neuropathic pain, acute intravenous resveratrol administration suppresses trigeminal sensory transmission, including nociception, and so resveratrol may be used as a complementary and alternative medicine therapeutic agent for the treatment of trigeminal nociceptive pain, including hyperalgesia.
authors.affiliation: Laboratory of Food and Physiological Sciences, Department of Life and Food Sciences, School of Life and Environmental Sciences, Azabu University, 1-17-71, Fuchinobe, Chuo-ku, Sagamihara, Kanagawa 252-5201, Japan.
authors.forename: Shiori
authors.initials: S
authors.lastname: Takehana
authors.affiliation: Laboratory of Food and Physiological Sciences, Department of Life and Food Sciences, School of Life and Environmental Sciences, Azabu University, 1-17-71, Fuchinobe, Chuo-ku, Sagamihara, Kanagawa 252-5201, Japan.
authors.forename: Kenta
authors.initials: K
authors.lastname: Sekiguchi
authors.affiliation: Laboratory of Physiology II, Department of Veterinary Science, School of Veterinary Medicine, Azabu University, 1-17-71, Fuchinobe, Chuo-ku, Sagamihara, Kanagawa 252-5201, Japan.
authors.forename: Maki
authors.initials: M
authors.lastname: Inoue
authors.affiliation: Fancl Health Science Research Center, Research Institute, FANCL Corporation, 12-13, Kamishinano, Totsuka-ku, Yokohama, Kanagawa 244-0806, Japan.
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Kubota
authors.affiliation: Fancl Health Science Research Center, Research Institute, FANCL Corporation, 12-13, Kamishinano, Totsuka-ku, Yokohama, Kanagawa 244-0806, Japan.
authors.forename: Yukihiko
authors.initials: Y
authors.lastname: Ito
authors.affiliation: Fancl Health Science Research Center, Research Institute, FANCL Corporation, 12-13, Kamishinano, Totsuka-ku, Yokohama, Kanagawa 244-0806, Japan.
authors.forename: Kei
authors.initials: K
authors.lastname: Yui
authors.affiliation: Laboratory of Food and Physiological Sciences, Department of Life and Food Sciences, School of Life and Environmental Sciences, Azabu University, 1-17-71, Fuchinobe, Chuo-ku, Sagamihara, Kanagawa 252-5201, Japan.
authors.forename: Yoshihito
authors.initials: Y
authors.lastname: Shimazu
authors.affiliation: Laboratory of Food and Physiological Sciences, Department of Life and Food Sciences, School of Life and Environmental Sciences, Azabu University, 1-17-71, Fuchinobe, Chuo-ku, Sagamihara, Kanagawa 252-5201, Japan. Electronic address: m-takeda@azabu-u.ac.jp.
authors.forename: Mamoru
authors.initials: M
authors.lastname: Takeda
mesh_terms: D000200:Action Potentials / Q000187:drug effects / Q000502:physiology; D061605:Administration, Intravenous; D000700:Analgesics / Q000008:administration & dosage*; D000818:Animals; D004305:Dose-Response Relationship, Drug; D005145:Face / Q000502:physiology; D008297:Male; D008839:Microelectrodes; D009474:Neurons / Q000187:drug effects* / Q000502:physiology; D059225:Nociception / Q000187:drug effects* / Q000502:physiology; D010812:Physical Stimulation; D017208:Rats, Wistar; D000077185:Resveratrol; D013267:Stilbenes / Q000008:administration & dosage*; D014110:Touch / Q000187:drug effects* / Q000502:physiology; D014275:Trigeminal Caudal Nucleus / Q000187:drug effects* / Q000503:physiopathology
chemical_list: D000700:Analgesics; D013267:Stilbenes; D000077185:Resveratrol
keywords: Alternative medicine; Nociception; Polyphenol; Resveratrol; Single unit recording; Spinal trigeminal nucleus caudalis