title: IMP3 Predicts Invasion and Prognosis in Human Lung Adenocarcinoma.
abstract: OBJECTIVE
  Insulin-like growth factor II mRNA-binding protein 3 (IMP3) is an oncofetal protein associated with several aggressive and advanced cancers. Whether IMP3 can predict invasion, and prognosis in patients with human lung adenocarcinoma (LAC) remains unclear.
  
  
  METHODS
  Ninety-five LAC and 75 non-tumor lung tissue samples were included in a tissue microarray. IMP3 expression was assessed by immunohistochemical examination. Correlation between IMP3 expression levels, clinicopathological characteristics, and overall prognosis was evaluated. In a separate in vitro study, RNA interference method was applied for knockdown of IMP3 gene in human LAC cell lines. Invasive potential of LAC cells was then evaluated by transwell migration assay.
  
  
  RESULTS
  IMP3 immunoreactivity was observed in 39 out of 95 (41.1 %) LAC patients, but not in non-tumor lung tissues. IMP3 expression levels were closely associated with histological grade (P = 0.037), TNM stage (P = 0.034), and lymph node metastasis (P = 0.011). Patients presenting with positive IMP3 expression (P = 0.000), an advanced TNM stage (P = 0.000), and lymph node metastasis (P = 0.001) had a worse overall survival, compared to those lacking these characteristics. Both IMP3 expression (hazard ratio [HR], 2.310; 95 % confidence interval [CI] 1.192-4.476; P = 0.013) and TNM stage (HR 2.338; 95 % CI 1.393-3.925; P = 0.001) were independent predictors of poor prognosis. The invasive potential of LAC cells was significantly inhibited by IMP3 knockdown.
  
  
  CONCLUSIONS
  IMP3 appears to play an important role in tumor invasion in patients with LAC and may serve as a useful prognostic biomarker in these patients.
authors.affiliation: Department of Pathology, School of Basic Medical Science, Southern Medical University, Guangzhou, China.|Department of Pathology, Guangdong General Hospital, Guangdong Academy of Medical Sciences, Guangzhou, China.
authors.forename: Jinhai
authors.initials: J
authors.lastname: Yan
authors.affiliation: Department of Pathology, The Third Affiliated Hospital of Southern Medical University, Zhongshan Dadaoxi, Street No. 183, Guangzhou, 510630, China.
authors.forename: Qingzhu
authors.initials: Q
authors.lastname: Wei
authors.affiliation: Department of Pathology, School of Basic Medical Science, Southern Medical University, Guangzhou, China.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Jian
authors.affiliation: Department of Pathology, School of Basic Medical Science, Southern Medical University, Guangzhou, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Qiu
authors.affiliation: Department of Pathology, School of Basic Medical Science, Southern Medical University, Guangzhou, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wen
authors.affiliation: Department of Pathology, The Third Affiliated Hospital of Southern Medical University, Zhongshan Dadaoxi, Street No. 183, Guangzhou, 510630, China.
authors.forename: Jianghuan
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Pathology, The Third Affiliated Hospital of Southern Medical University, Zhongshan Dadaoxi, Street No. 183, Guangzhou, 510630, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Fu
authors.affiliation: Department of Pathology, School of Basic Medical Science, Southern Medical University, Guangzhou, China.
authors.forename: Xinhua
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Department of Pathology, The Third Affiliated Hospital of Southern Medical University, Zhongshan Dadaoxi, Street No. 183, Guangzhou, 510630, China. zhaotongketizu@126.com.
authors.forename: Tong
authors.initials: T
authors.lastname: Zhao
mesh_terms: D000230:Adenocarcinoma / Q000737:chemistry* / Q000556:secondary*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D005260:Female; D055785:Gene Knockdown Techniques; D006801:Humans; D008168:Lung / Q000737:chemistry; D008175:Lung Neoplasms / Q000737:chemistry* / Q000473:pathology*; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D060787:Neoplasm Grading; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D011379:Prognosis; D016601:RNA-Binding Proteins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D015996:Survival Rate; D055815:Young Adult
chemical_list: C498780:IGF2BP3 protein, human; D016601:RNA-Binding Proteins
keywords: IMP3; Invasion; Lung adenocarcinoma; Prognosis; RNA interference