title: Size-dependent effects of layered double hydroxide nanoparticles on cellular functions of mouse embryonic stem cells.
abstract: OBJECTIVE
  Layered double hydroxide nanoparticles (LDH NPs) are promising for stem cell research and applications. In this study, we investigated the size-dependent interactions of LDH NPs with mouse embryonic stem cells (mESCs).
  
  
  METHODS
  LDH NPs with diameters of 100 and 50 nm were synthesized and characterized. mESCs were cultured to undergo spontaneous differentiation. After incubation with LDH NPs, cytotoxicity, cellular uptake, pluripotency, differentiation and epithelial-mesenchymal transition process of mESCs were assessed.
  
  
  RESULTS
  LDH NPs with the size of 50 nm induced higher cellular uptake and more outstandingly inhibited spontaneous differentiation and epithelial-mesenchymal transition process.
  
  
  CONCLUSIONS
  Our research demonstrated the size-dependent effects of LDH NPs on controlling fate and cellular functions of mESCs.
authors.affiliation: Tenth People's Hospital, School of Life Science and Technology, Tongji University, Shanghai 200092, PR China.
authors.forename: Youjun
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Tenth People's Hospital, School of Life Science and Technology, Tongji University, Shanghai 200092, PR China.
authors.forename: Rongrong
authors.initials: R
authors.lastname: Zhu
authors.affiliation: Tenth People's Hospital, School of Life Science and Technology, Tongji University, Shanghai 200092, PR China.
authors.forename: Xin
authors.initials: X
authors.lastname: Ge
authors.affiliation: Tenth People's Hospital, School of Life Science and Technology, Tongji University, Shanghai 200092, PR China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Sun
authors.affiliation: Tenth People's Hospital, School of Life Science and Technology, Tongji University, Shanghai 200092, PR China.
authors.forename: Zhaoqi
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Tenth People's Hospital, School of Life Science and Technology, Tongji University, Shanghai 200092, PR China.
authors.forename: Wenrui
authors.initials: W
authors.lastname: Wang
authors.affiliation: Tenth People's Hospital, School of Life Science and Technology, Tongji University, Shanghai 200092, PR China.
authors.forename: Mei
authors.initials: M
authors.lastname: Wang
authors.affiliation: Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200438, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Liu
authors.affiliation: Tenth People's Hospital, School of Life Science and Technology, Tongji University, Shanghai 200092, PR China.
authors.forename: Shilong
authors.initials: S
authors.lastname: Wang
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D058750:Epithelial-Mesenchymal Transition / Q000187:drug effects; D006878:Hydroxides / Q000737:chemistry*; D051379:Mice; D000066450:Mouse Embryonic Stem Cells / Q000166:cytology; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size
chemical_list: D006878:Hydroxides; C031356:hydroxide ion
keywords: embryonic stem cells; epithelial–mesenchymal transition; layered double hydroxide nanoparticles; size-dependence; spontaneous differentiation