title: MiR-203 Determines Poor Outcome and Suppresses Tumor Growth by Targeting TBK1 in Osteosarcoma.
abstract: OBJECTIVE
  Increasing evidence has shown that miR-203 plays important role in human cancer progression. However, little is known about the function of miR-203 in osteosarcoma (OS).
  
  
  METHODS
  The expression of miR-203 in OS tissues and cell lines were examined by qRT-PCR. The biological role of miR-20 in OS cell proliferation was examined in vitro and in vivo. The targets of miR-203 were identified by a luciferase reporter gene assay.
  
  
  RESULTS
  miR-203 was down regulated in OS tissues and cell lines; decreased miR-203 was associated with a poor overall survival in OS patients. Restoration of miR-203 expression reduced cell growth in vitro and suppressed tumorigenicity in vivo. In contrast, inhibition of miR-203 stimulated OS cell growth both in vitro and in vivo. In addition, TANK binding kinase 1 (TBK1) was identified as a direct target of miR-203; overexpression of TBK1 partly reversed the suppressive effects of miR-203. Furthermore, TBK1 was found up-regulated and inversely correlated with miR-203 in OS tissues.
  
  
  CONCLUSIONS
  Taken together, these findings suggest that miR-203 acts as a tumor suppressor via regulation of TBK1 expression in OS progression, and miR-203 may be a promising therapeutic target for OS.
authors.forename: Shiping
authors.initials: S
authors.lastname: Liu
authors.forename: Peng
authors.initials: P
authors.lastname: Feng
mesh_terms: D020413:3' Untranslated Regions; D000293:Adolescent; D000328:Adult; D000818:Animals; D001483:Base Sequence; D001859:Bone Neoplasms / Q000235:genetics / Q000401:mortality / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002648:Child; D015536:Down-Regulation; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D016376:Oligonucleotides, Antisense / Q000378:metabolism; D012516:Osteosarcoma / Q000235:genetics / Q000401:mortality / Q000473:pathology*; D017346:Protein Serine-Threonine Kinases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D015996:Survival Rate; D014183:Transplantation, Heterologous; D015854:Up-Regulation
chemical_list: D020413:3' Untranslated Regions; C546703:MIRN203 microRNA, human; D035683:MicroRNAs; D016376:Oligonucleotides, Antisense; D017346:Protein Serine-Threonine Kinases; C403191:TBK1 protein, human