title: [Effect of Shenxiong injection on inflammation injury of ischemia-reperfusion injury rats].
abstract: To investigate the effect of Shenxiong injection on the inflammation injury of ischemia-reperfusion injury senile rats. Totally 84 Sprague-Dawley (SD) rats were randomly divided into six groups: the sham operation group, the model group, the Nimodipine group and the Shenxiong injection(low, middle, and high dosage) groups. The rat cerebral ischemia-reperfusion model was established through intraperitoneal injection for 3 d and middle cerebral artery occlusion (MCAO). Ater the reperfusion for 24 h, efforts were made to give neurological score, collect brains for TTC staining, detect tumor necrosis factor-α(TNF-α) and interleukin-1β(IL-1β) content in serum by enzyme-linked immunosorbent assay (ELISA) method and measure IL-1β, ICAM-1 and MMP-9 mRNA expressions in hippocampal area by Real-time PCR (RT-PCR). According to the results, Shenxiong injection could decrease the cerebral infarction volume, greatly improved the neurological function and reduce IL-1β, TNF-α, ICAM-1 and MMP-9 mRNA expressions and IL-1β and TNF-α contents. In conclusion, Shenxiong injection shows the significant protective effect on ischemia-reperfusion injury in rats. Its mechanism may be related to the inhibition of inflammatory factor expression.
authors.forename: Hui-fen
authors.initials: HF
authors.lastname: Zhou
authors.forename: Jin-chao
authors.initials: JC
authors.lastname: Al
authors.forename: Hai-tong
authors.initials: HT
authors.lastname: Wan
authors.forename: Yu
authors.initials: Y
authors.lastname: He
authors.forename: Yu-yan
authors.initials: YY
authors.lastname: Zhang
authors.forename: Tao
authors.initials: T
authors.lastname: Zhao
authors.forename: Wei
authors.initials: W
authors.lastname: Fu
authors.forename: Jie-hong
authors.initials: JH
authors.lastname: Yang
mesh_terms: D000818:Animals; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics / Q000276:immunology; D016898:Interferon-alpha / Q000235:genetics / Q000276:immunology; D053583:Interleukin-1beta / Q000235:genetics / Q000276:immunology; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000276:immunology; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000235:genetics / Q000276:immunology*
chemical_list: D004365:Drugs, Chinese Herbal; D016898:Interferon-alpha; D053583:Interleukin-1beta; D018799:Intercellular Adhesion Molecule-1; D020780:Matrix Metalloproteinase 9