title: Fibrinogen Demonstration in Oral Lichen Planus: An Immunofluorescence Study on Archival Tissues.
abstract: Lichen planus is a premalignant condition with minimal diagnostic aids. This study is an attempt to use paraffin embedded sections of lichen planus with immunofluorescein stain and to evaluate the immunofluorescent sections to establish pattern of fibrinogen deposition.
  
  
  
  Thirty-five paraffin embedded sections of old and new cases of oral lichen planus (study group) and five normal oral mucosa (control group) were chosen. Two sections of each (H & E) case were taken, one was stained with hematoxylin and eosin and another with fluorescein isothiocynate conjugate (FITC) polyclonal rabbit antibody against fibrinogen. Fluorescent findings were examined with a fluorescent microscope.
  
  
  
  A high statistical significant correlation was found in respect to fluorescence positivity, intensity of fluorescence and distribution of fluorescence each with p < 0.0001 and fluorescence at blood vessel walls (p = 0.0003).
  
  
  
  This study suggested that paraffin embedded sections can be successfully used in direct immunofluorescence staining in routine set up where only formalin fixed tissues are received.
  
  
  
  Paraffin embedded sections can be successfully used in direct immunofluorescence staining when only formalin fixed tissues are received.
authors.affiliation: Reader, Department of Oral Pathology, MA Rangoonwala College of Dental Sciences & Research Centre, Pune, Maharashtra, India, Phone: 9970133664, e-mail: pdshirol@yahoo.com.
authors.forename: Pallavi D
authors.initials: PD
authors.lastname: Shirol
authors.affiliation: Department of Oral Pathology, KLES Institute of Dental Sciences, Belgaum, Karnataka, India.
authors.forename: Veena
authors.initials: V
authors.lastname: Naik
authors.affiliation: Department of Oral Pathology, KLES Institute of Dental Sciences, Belgaum, Karnataka, India.
authors.forename: Alka
authors.initials: A
authors.lastname: Kale
mesh_terms: D005340:Fibrinogen; D005455:Fluorescent Antibody Technique; D006801:Humans; D017676:Lichen Planus, Oral; D009061:Mouth Mucosa; D013048:Specimen Handling
chemical_list: D005340:Fibrinogen
keywords: Direct immunofluorescence; Fibrinogen; Oral lichen planus; Paraffin sections