title: High Sensitivity Measurement of Pancreatic Polypeptide and Its Variant in Serum and Plasma by LC-MS/MS.
abstract: Aliquots of serum or plasma samples are combined with stable isotope labeled internal standard. Pancreatic polypeptide (PP) and its truncated variant PP3-36 are enriched by incubation with anti-PP antibody conjugated to magnetic beads. Peptides are eluted from beads in acidic buffer and the samples analyzed using liquid chromatography coupled with tandem mass spectrometry. Instrumental analysis of PP and PP3-36 is performed using electrospray ionization ESI in positive ion mode and multiple reaction monitoring (MRM) acquisition.
authors.affiliation: ARUP Institute for Clinical and Experimental Pathology, ARUP Laboratories, 200 2nd St. SW, Salt Lake City, UT, 55905, USA.
authors.forename: Hernando
authors.initials: H
authors.lastname: Escobar
authors.affiliation: ARUP Institute for Clinical and Experimental Pathology, ARUP Laboratories, 200 2nd St. SW, Salt Lake City, UT, 55905, USA.
authors.forename: Mark M
authors.initials: MM
authors.lastname: Kushnir
authors.affiliation: ARUP Institute for Clinical and Experimental Pathology, ARUP Laboratories, 200 2nd St. SW, Salt Lake City, UT, 55905, USA. alan.rockwood@aruplab.com.|Department of Pathology, University of Utah School of Medicine, Salt Lake City, UT, USA. alan.rockwood@aruplab.com.
authors.forename: Alan L
authors.initials: AL
authors.lastname: Rockwood
authors.affiliation: ARUP Institute for Clinical and Experimental Pathology, ARUP Laboratories, 200 2nd St. SW, Salt Lake City, UT, 55905, USA.|Department of Pathology, University of Utah School of Medicine, Salt Lake City, UT, USA.|Department of Medicine, University of Utah School of Medicine, Salt Lake City, UT, USA.
authors.forename: A Wayne
authors.initials: AW
authors.lastname: Meikle
mesh_terms: D001774:Blood Chemical Analysis / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D057230:Limit of Detection; D010191:Pancreatic Polypeptide / Q000097:blood* / Q000737:chemistry; D010446:Peptide Fragments / Q000097:blood*; D013223:Statistics as Topic; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D010446:Peptide Fragments; D010191:Pancreatic Polypeptide
keywords: Clinic biomarker; Mass spectrometry; Pancreatic polypeptide; Peptide hormone; Tandem mass spectrometry