title: Case Report Associated with Aspergillosis and Hepatitis E Virus Coinfection in Himalayan Griffons.
abstract: This study involved a death which occurred in four Himalayan griffons housed in Beijing zoo, China. Based on pathogen identification and the pathological changes observed, we did characterize the fungi and Hepatitis E virus (HEV) in four dead Himalayan griffons. Pathological changes were severe. Membranous-like material was observed on the surface of the internal organs. Spleen was necrotic. Focal lymphocyte infiltration in the liver and many sunflower-like fungi nodules were evident in the tissues, especially in the kidney. PCR was used to identify the pathogen. Based on the 18SrRNA genomic sequence of known fungi, the results confirmed that all four dead Himalayan griffons were infected with Aspergillus. At the same time the detection of HEV also showed positive results. To the best of our knowledge, this work appears to be the first report of concurrent presence of Aspergillosis and Hepatitis E virus in rare avian species.
authors.affiliation: Laboratory of Veterinary Pathology and Public Health, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
authors.forename: Heng
authors.initials: H
authors.lastname: Li
authors.affiliation: Laboratory of Veterinary Pathology and Public Health, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
authors.forename: Rining
authors.initials: R
authors.lastname: Zhu
authors.affiliation: Laboratory of Veterinary Pathology and Public Health, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
authors.forename: Ruiping
authors.initials: R
authors.lastname: She
authors.affiliation: Beijing Zoo, Beijing, China.
authors.forename: Chenglin
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Laboratory of Veterinary Pathology and Public Health, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
authors.forename: Ruihan
authors.initials: R
authors.lastname: Shi
authors.affiliation: Laboratory of Veterinary Pathology and Public Health, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: Laboratory of Veterinary Pathology and Public Health, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Du
authors.affiliation: Laboratory of Veterinary Pathology and Public Health, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
authors.forename: Qiaoxing
authors.initials: Q
authors.lastname: Wu
authors.affiliation: Laboratory of Veterinary Pathology and Public Health, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
authors.forename: Fengjiao
authors.initials: F
authors.lastname: Hu
authors.affiliation: Laboratory of Veterinary Pathology and Public Health, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Laboratory of Veterinary Pathology and Public Health, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
authors.forename: Majid Hussain
authors.initials: MH
authors.lastname: Soomro
authors.affiliation: Beijing Zoo, Beijing, China.
authors.forename: Changming
authors.initials: C
authors.lastname: Zheng
mesh_terms: D000818:Animals; D001228:Aspergillosis / Q000662:veterinary / Q000821:virology; D001715:Bird Diseases / Q000382:microbiology / Q000821:virology; D002681:China; D060085:Coinfection; D046093:Falconiformes; D016751:Hepatitis E / Q000382:microbiology / Q000662:veterinary; D052678:Hepevirus