title: The Induction Effect of Am80 and TSA on ESC Differentiation via Regulation of Stra8 in Chicken.
abstract: Stra8 encodes stimulated by retinoic acid gene 8, a protein that is important for initiation of meiosis in mammals and birds. This study was aimed at identifying the active control area of chicken STRA8 gene core promoter, to screen optimum inducers of the STRA8 gene, thus to enhance the differentiation of embryonic stem cells (ESCs) into spermatogonial stem cells. Fragments of chicken STRA8 gene promoter were cloned into fluorescent reporter plasmids and transfected into DF-1 cells. Then Dual-Luciferase® Reporter Assay System was used to identify the activity of the STRA8 gene under different inducers. Our studies showed that the promoter fragment -1055 bp to +54 bp of Suqin chicken Stra8 revealed the strongest activity. The dual-luciferase® reporter showed that Tamibarotene (Am80) and TrichostatinA (TSA) could significantly enhance STRA8 transcription. The in vitro inductive culture of chicken ESCs demonstrated that spermatogonial stem cells (SSC)-like cells appeared and Integrinβ1 protein was expressed on day 10, indicating that Am80 and TSA can promote ESCs differentiation into SSCs via regulation of Stra8.
authors.affiliation: College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.|Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, Yangzhou 225009, China.
authors.forename: Yani
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.|Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, Yangzhou 225009, China.
authors.forename: Qisheng
authors.initials: Q
authors.lastname: Zuo
authors.affiliation: College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.|Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, Yangzhou 225009, China.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Liu
authors.affiliation: College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.|Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, Yangzhou 225009, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Li
authors.affiliation: College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.|Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, Yangzhou 225009, China.
authors.forename: Beibei
authors.initials: B
authors.lastname: Tang
authors.affiliation: College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.|Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, Yangzhou 225009, China.
authors.forename: Tian-Rong
authors.initials: TR
authors.lastname: Xiao
authors.affiliation: College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.|Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, Yangzhou 225009, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Lian
authors.affiliation: College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.|Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, Yangzhou 225009, China.
authors.forename: Yingjie
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.|Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, Yangzhou 225009, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Jin
authors.affiliation: College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.|Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, Yangzhou 225009, China.
authors.forename: Yilin
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.
authors.forename: Wenhui
authors.initials: W
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.|Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, Yangzhou 225009, China.
authors.forename: Bichun
authors.initials: B
authors.lastname: Li
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D030161:Avian Proteins / Q000235:genetics / Q000378:metabolism*; D001565:Benzoates / Q000494:pharmacology*; D002454:Cell Differentiation; D002642:Chick Embryo; D002645:Chickens; D053595:Embryonic Stem Cells / Q000502:physiology*; D056572:Histone Deacetylase Inhibitors / Q000494:pharmacology*; D006877:Hydroxamic Acids / Q000494:pharmacology*; D011401:Promoter Regions, Genetic; D013764:Tetrahydronaphthalenes / Q000494:pharmacology*; D046509:Tissue Culture Techniques; D015533:Transcriptional Activation / Q000187:drug effects
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D030161:Avian Proteins; D001565:Benzoates; D056572:Histone Deacetylase Inhibitors; D006877:Hydroxamic Acids; D013764:Tetrahydronaphthalenes; C061133:tamibarotene; C012589:trichostatin A