title: Estrogen Receptor Agonists and Antagonists in the Yeast Estrogen Bioassay.
abstract: Cell-based bioassays can be used to predict the eventual biological activity of a substance on a living organism. In vitro reporter gene bioassays are based on recombinant vertebrate cell lines or yeast strains and especially the latter are easy-to-handle, cheap, and fast. Moreover, yeast cells do not express estrogen, androgen, progesterone or glucocorticoid receptors, and are thus powerful tools in the development of specific reporter gene systems that are devoid of crosstalk from other hormone pathways. This chapter describes our experience with an in-house developed RIKILT yeast estrogen bioassay for testing estrogen receptor agonists and antagonists, focusing on the applicability of the latter.
authors.affiliation: Business Unit of Toxicology & Bioassays, RIKILT-Institute of Food Safety, Wageningen University and Research Centre, Akkermaalsbos 2, 6708 WB, Wageningen, The Netherlands.
authors.forename: Si
authors.initials: S
authors.lastname: Wang
authors.affiliation: Business Unit of Toxicology & Bioassays, RIKILT-Institute of Food Safety, Wageningen University and Research Centre, Akkermaalsbos 2, 6708 WB, Wageningen, The Netherlands. toine.bovee@wur.nl.
authors.forename: Toine F H
authors.initials: TFH
authors.lastname: Bovee
mesh_terms: D001681:Biological Assay / Q000379:methods*; D004965:Estrogen Antagonists / Q000494:pharmacology*; D047628:Estrogen Receptor alpha / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D004967:Estrogens / Q000494:pharmacology*; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008024:Ligands; D020218:Response Elements; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D057188:Workflow
chemical_list: C506487:ESR1 protein, human; D004965:Estrogen Antagonists; D047628:Estrogen Receptor alpha; D004967:Estrogens; D008024:Ligands; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins
keywords: Estrogen antagonist; Estrogen receptor; In vitro; Yeast estrogen bioassay