title: Morphological Criteria of Cell Differentiation Stages in Experimental Hepatocarcinoma and Evaluation of Antitumor Drug Efficiency.
abstract: Structural polymorphism of 5 cell differentiation stages of hepatocarcinoma-29 from ascitic fluid is detected and the morphological criteria for identification of these stages are defined on the base of optic and electron microscopy findings, cytofluorometry, and DNA cytometry. The percentage of cells at differentiation stages 4 and 5 in the tumor structure increases after hepatocarcinoma cell inoculation into the hip. Injection of a cell cycle-modulating substance to animals with tumor growth shifts the proportion of cells with various differentiation stages. The morphological criteria of 5 stages of hepatocarcinoma-29 cell differentiation can be used for prospective drug testing.
authors.affiliation: Research Institute of Clinical and Experimental Lymphology, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia. n_bgatova@ngs.ru.
authors.forename: N P
authors.initials: NP
authors.lastname: Bgatova
authors.affiliation: Institute of Molecular and Cellular Biology, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia.
authors.forename: L V
authors.initials: LV
authors.lastname: Omel'yanchuk
authors.affiliation: Research Institute of Clinical and Experimental Lymphology, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia.
authors.forename: A A
authors.initials: AA
authors.lastname: Pozhidaeva
authors.affiliation: Institute of Molecular and Cellular Biology, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia.
authors.forename: V F
authors.initials: VF
authors.lastname: Semeshin
authors.affiliation: Research Institute of Clinical and Experimental Lymphology, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia.
authors.forename: A P
authors.initials: AP
authors.lastname: Lykov
authors.affiliation: Research Institute of Clinical and Experimental Lymphology, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia.
authors.forename: O V
authors.initials: OV
authors.lastname: Poveshchenko
authors.affiliation: Research Institute of Clinical and Experimental Lymphology, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia.
authors.forename: O P
authors.initials: OP
authors.lastname: Makarova
authors.affiliation: Research Institute of Clinical and Experimental Lymphology, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia.
authors.forename: L N
authors.initials: LN
authors.lastname: Rachkovskaya
authors.affiliation: Research Institute of Clinical and Experimental Lymphology, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia.
authors.forename: Yu I
authors.initials: YI
authors.lastname: Borodin
authors.affiliation: Research Institute of Clinical and Experimental Lymphology, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia.
authors.forename: V I
authors.initials: VI
authors.lastname: Konenkov
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use; D017209:Apoptosis; D001202:Ascitic Fluid / Q000473:pathology; D002453:Cell Cycle / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002467:Cell Nucleus / Q000648:ultrastructure; D048429:Cell Size; D002951:Citrates / Q000494:pharmacology; D004273:DNA, Neoplasm / Q000032:analysis; D004354:Drug Screening Assays, Antitumor; D008114:Liver Neoplasms, Experimental / Q000188:drug therapy / Q000473:pathology*; D051379:Mice; D008808:Mice, Inbred CBA; D009368:Neoplasm Transplantation; D011003:Ploidies
chemical_list: D000970:Antineoplastic Agents; D002951:Citrates; D004273:DNA, Neoplasm; C070669:lithium citrate
keywords: differentiation stages; hepatocarcinoma-29; morphological criteria