title: Rapid Identification and Classification of Listeria spp. and Serotype Assignment of Listeria monocytogenes Using Fourier Transform-Infrared Spectroscopy and Artificial Neural Network Analysis.
abstract: The use of Fourier Transform-Infrared Spectroscopy (FT-IR) in conjunction with Artificial Neural Network software NeuroDeveloper™ was examined for the rapid identification and classification of Listeria species and serotyping of Listeria monocytogenes. A spectral library was created for 245 strains of Listeria spp. to give a biochemical fingerprint from which identification of unknown samples were made. This technology was able to accurately distinguish the Listeria species with 99.03% accuracy. Eleven serotypes of Listeria monocytogenes including 1/2a, 1/2b, and 4b were identified with 96.58% accuracy. In addition, motile and non-motile forms of Listeria were used to create a more robust model for identification. FT-IR coupled with NeuroDeveloper™ appear to be a more accurate and economic choice for rapid identification of pathogenic Listeria spp. than current methods.
authors.affiliation: Department of Biological Sciences, California State University East Bay, Hayward, CA, United States of America.|United States Department of Agriculture, Agricultural Research Service, Western Regional Research Center, Albany, CA, United States of America.
authors.forename: K F
authors.initials: KF
authors.lastname: Romanolo
authors.affiliation: United States Department of Agriculture, Agricultural Research Service, Western Regional Research Center, Albany, CA, United States of America.
authors.forename: L
authors.initials: L
authors.lastname: Gorski
authors.affiliation: Bruker Optics Inc, Fremont, CA, United States of America.
authors.forename: S
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Biological Sciences, California State University East Bay, Hayward, CA, United States of America.
authors.forename: C R
authors.initials: CR
authors.lastname: Lauzon
mesh_terms: D008089:Listeria monocytogenes / Q000145:classification* / Q000302:isolation & purification*; D016571:Neural Networks, Computer; D012703:Serotyping; D013045:Species Specificity; D017550:Spectroscopy, Fourier Transform Infrared