title: NK cell compartment in the peripheral blood and spleen in adult patients with primary immune thrombocytopenia.
abstract: Immune thrombocytopenic purpura (ITP) is a disease characterized by antibody-mediated platelet destruction. The T- and B-cell subsets have been extensively studied in primary ITP, but the NK cell compartment has been less thoroughly explored. We investigated the NK cell receptor repertoire and the functionality of NK cells in the peripheral blood and spleen in patients with primary ITP. An immunophenotypic analysis of peripheral blood lymphocytes from patients revealed that the numbers of CD19+B lymphocytes, CD4+and CD8+T lymphocytes and CD3-CD56+NK cells were within the normal range. No major alteration to the expression of distinct inhibitory or activating NK cell receptors was observed. The functionality of NK cells, as evaluated by their ability to degranulate in conditions of natural cytotoxicity or antibody-dependent cell cytotoxicity (ADCC), was preserved in these patients. By contrast, these stimuli induced lower levels of IFNγ production by the NK cells of ITP patients than by those of healthy controls. We then compared the splenic NK cell functions of ITP patients with those of cadaveric heart-beating donors (CHBD) as controls. The splenic NK cells of ITP patients tended to be less efficient in natural cytotoxicity conditions and more efficient in ADCC conditions than control splenic NK cells. Finally, we found that infusions of intravenous immunoglobulin led to the inhibition of NK cell activation through the modulation of the interface between target cells and NK cells.
authors.affiliation: Département de Médecine Interne, Hôpital Timone Adulte, Assistance Publique - Hôpitaux de Marseille, Aix-Marseille Université, 13005 Marseille, France.
authors.forename: M
authors.initials: M
authors.lastname: Ebbo
authors.affiliation: Immunologie, Hôpital de la Conception, Assistance Publique - Hôpitaux de Marseille, Aix-Marseille Université, 13005 Marseille, France.
authors.forename: S
authors.initials: S
authors.lastname: Audonnet
authors.affiliation: Département de Médecine Interne, Hôpital Timone Adulte, Assistance Publique - Hôpitaux de Marseille, Aix-Marseille Université, 13005 Marseille, France.
authors.forename: A
authors.initials: A
authors.lastname: Grados
authors.affiliation: Département de Médecine Interne, Hôpital Timone Adulte, Assistance Publique - Hôpitaux de Marseille, Aix-Marseille Université, 13005 Marseille, France.
authors.forename: L
authors.initials: L
authors.lastname: Benarous
authors.affiliation: Department of Internal Medicine, National Referral Center For Adult Immune Cytopenias, Henri-Mondor University Hospital, Assistance Publique - Hôpitaux de Paris, Université Paris-Est Créteil, 51 Av. du Mal de Lattre de Tassigny, 94010 Créteil cedex, France.
authors.forename: M
authors.initials: M
authors.lastname: Mahevas
authors.affiliation: Department of Internal Medicine, National Referral Center For Adult Immune Cytopenias, Henri-Mondor University Hospital, Assistance Publique - Hôpitaux de Paris, Université Paris-Est Créteil, 51 Av. du Mal de Lattre de Tassigny, 94010 Créteil cedex, France.
authors.forename: B
authors.initials: B
authors.lastname: Godeau
authors.affiliation: Département de Médecine Interne, CHU de Bordeaux-GH Sud, Hôpital Haut-Lévêque 1 avenue Magellan, 33604 Pessac cedex, France.
authors.forename: J F
authors.initials: JF
authors.lastname: Viallard
authors.affiliation: Immunologie, Hôpital de la Conception, Assistance Publique - Hôpitaux de Marseille, Aix-Marseille Université, 13005 Marseille, France.
authors.forename: C
authors.initials: C
authors.lastname: Piperoglou
authors.affiliation: Immunologie, Hôpital de la Conception, Assistance Publique - Hôpitaux de Marseille, Aix-Marseille Université, 13005 Marseille, France.
authors.forename: C
authors.initials: C
authors.lastname: Cognet
authors.affiliation: Immunologie, Hôpital de la Conception, Assistance Publique - Hôpitaux de Marseille, Aix-Marseille Université, 13005 Marseille, France.
authors.forename: C
authors.initials: C
authors.lastname: Farnarier
authors.affiliation: Département de Médecine Interne, Hôpital Timone Adulte, Assistance Publique - Hôpitaux de Marseille, Aix-Marseille Université, 13005 Marseille, France.
authors.forename: J R
authors.initials: JR
authors.lastname: Harlé
authors.affiliation: Département de Médecine Interne, Hôpital Timone Adulte, Assistance Publique - Hôpitaux de Marseille, Aix-Marseille Université, 13005 Marseille, France.
authors.forename: N
authors.initials: N
authors.lastname: Schleinitz
authors.affiliation: Centre d'Immunologie de Marseille-Luminy, Aix-Marseille University UM2, Inserm, U1104, CNRS UMR7280, 13288 Marseille, France; Immunologie, Hôpital de la Conception, Assistance Publique - Hôpitaux de Marseille, Aix-Marseille Université, 13005 Marseille, France. Electronic address: vely@ciml.univ-mrs.fr.
authors.forename: F
authors.initials: F
authors.lastname: Vély
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D016756:Immunoglobulins, Intravenous / Q000494:pharmacology / Q000627:therapeutic use; D007155:Immunologic Factors / Q000494:pharmacology / Q000627:therapeutic use; D007371:Interferon-gamma / Q000097:blood / Q000276:immunology; D020014:K562 Cells; D007694:Killer Cells, Natural / Q000187:drug effects / Q000276:immunology*; D007963:Leukocytes, Mononuclear; D008297:Male; D051379:Mice; D008875:Middle Aged; D016553:Purpura, Thrombocytopenic, Idiopathic / Q000097:blood / Q000188:drug therapy / Q000276:immunology*; D013154:Spleen / Q000166:cytology / Q000276:immunology; D055815:Young Adult
chemical_list: D016756:Immunoglobulins, Intravenous; D007155:Immunologic Factors; D007371:Interferon-gamma