title: Site-Directed RNA Editing in Vivo Can Be Triggered by the Light-Driven Assembly of an Artificial Riboprotein.
abstract: Site-directed RNA editing allows for the manipulation of RNA and protein function by reprogramming genetic information at the RNA level. For this we assemble artificial RNA-guided editases and demonstrate their transcript repair activity in cells and in developing embryos of the annelid Platynereis dumerilii. A hallmark of our assembly strategy is the covalent attachment of guideRNA and editing enzyme by applying the SNAP-tag technology, a process that we demonstrate here to be readily triggered by light in vitro, in mammalian cell culture, and also in P. dumerilii. Lacking both sophisticated chemistry and extensive genetic engineering, this technology provides a convenient route for the light-dependent switching of protein isoforms. The presented strategy may also serve as a blue-print for the engineering of addressable machineries that apply tailored nucleic acid analogues to manipulate RNA or DNA site-specifically in living organisms.
authors.affiliation: Interfaculty Institute of Biochemistry, University of Tübingen , Auf der Morgenstelle 15, 72076 Tübingen, Germany.
authors.forename: Alfred
authors.initials: A
authors.lastname: Hanswillemenke
authors.affiliation: Interfaculty Institute of Biochemistry, University of Tübingen , Auf der Morgenstelle 15, 72076 Tübingen, Germany.
authors.forename: Tahsin
authors.initials: T
authors.lastname: Kuzdere
authors.affiliation: Interfaculty Institute of Biochemistry, University of Tübingen , Auf der Morgenstelle 15, 72076 Tübingen, Germany.
authors.forename: Paul
authors.initials: P
authors.lastname: Vogel
authors.affiliation: Max-Planck-Institute for Developmental Biology , Spemannstraße 35, 72076 Tübingen, Germany.
authors.forename: Gáspár
authors.initials: G
authors.lastname: Jékely
authors.affiliation: Interfaculty Institute of Biochemistry, University of Tübingen , Auf der Morgenstelle 15, 72076 Tübingen, Germany.
authors.forename: Thorsten
authors.initials: T
authors.lastname: Stafforst
mesh_terms: D000818:Animals; D000845:Annelida / Q000196:embryology; D008027:Light; D017393:RNA Editing; D012269:Ribosomal Proteins / Q000737:chemistry*
chemical_list: D012269:Ribosomal Proteins