title: Rv3369 Induces Cytokine Interleukin-1β Production and Enhances Mycobacterium smegmatis Intracellular Survival.
abstract: The pathogenesis of tuberculosis, caused by Mycobacterium tuberculosis, is largely because of the pathogen's successful entry and survival within macrophages. We predicted that rv3369, a gene encoding a conserved protein, might play a role in the interactions with host cells. To test this, rv3369 gene was heterologously expressed in a nonpathogenic fast-growing Mycobacterium smegmatis strain. The recombinant strain survives better than the control within macrophages, accompanied by more host cell death and a marked higher secretion of interleukin-1β (IL-1β). Furthermore, pharmacological inhibition experiments showed that the NF-κB and ERK pathways were involved in the Rv3369-triggered IL-1β changes. These results provided evidence for the engagement of Rv3369 in the interaction between mycobacteria and host.
authors.affiliation: 1 Institute of Modern Biopharmaceuticals, State Key Laboratory Breeding Base of Eco-Environment and Bio-Resource of the Three Gorges Area, Key Laboratory of Ministry of Education Eco-Environment of the Three Gorges Reservoir Region, School of Life Sciences, Southwest University , Chongqing, China .|2 College of Life Sciences, Neijiang Normal University , Neijiang, China .
authors.forename: Wu
authors.initials: W
authors.lastname: Li
authors.affiliation: 1 Institute of Modern Biopharmaceuticals, State Key Laboratory Breeding Base of Eco-Environment and Bio-Resource of the Three Gorges Area, Key Laboratory of Ministry of Education Eco-Environment of the Three Gorges Reservoir Region, School of Life Sciences, Southwest University , Chongqing, China .
authors.forename: Minqiang
authors.initials: M
authors.lastname: Liu
authors.affiliation: 1 Institute of Modern Biopharmaceuticals, State Key Laboratory Breeding Base of Eco-Environment and Bio-Resource of the Three Gorges Area, Key Laboratory of Ministry of Education Eco-Environment of the Three Gorges Reservoir Region, School of Life Sciences, Southwest University , Chongqing, China .
authors.forename: Jianping
authors.initials: J
authors.lastname: Xie
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002470:Cell Survival / Q000276:immunology; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis; D048049:Extracellular Signal-Regulated MAP Kinases; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D053583:Interleukin-1beta / Q000096:biosynthesis*; D008264:Macrophages / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D050296:Microbial Viability; D009165:Mycobacterium Infections, Nontuberculous / Q000378:metabolism / Q000382:microbiology; D020102:Mycobacterium smegmatis / Q000502:physiology*; D016328:NF-kappa B / Q000378:metabolism; D020298:U937 Cells
chemical_list: D001426:Bacterial Proteins; D016207:Cytokines; D053583:Interleukin-1beta; D016328:NF-kappa B; D048049:Extracellular Signal-Regulated MAP Kinases