title: Downregulated Expression of E-cadherin and TP53 in Patients with Gastric Diseases: the Involvement of H. pylori Infection and Its Virulence Markers.
abstract: OBJECTIVE
  Gastritis caused by infection with Helicobacter pylori is characterized by chronic inflammation and damage in gastric tissue, which is a main risk factor for gastric cancer. Associated with H. pylori, the TP53 gene tumor suppressor and the cell adhesion glycoprotein epithelial cadherin develop a relevant role in the integrity and carcinogenesis of the epithelium. We aimed to detection of H. pylori and its main virulence markers and measured the messenger RNA (mRNA) expression levels of E-cadherin and TP53 genes.
  
  
  METHODS
  The detection of H. pylori and its virulence markers, as well as the mRNA expression levels of E-cadherin and TP53 genes, were obtained for 161 samples of gastric biopsies including 37 with normal gastric tissue, 70 with gastritis, 24 from neoplastic tissue, and 27 from adjacent non-neoplastic by means of a quantitative real-time polymerase chain reaction.
  
  
  RESULTS
  The mRNA expression levels of E-cadherin and TP53 were found to be decreased in patients with gastritis, independently of H. pylori infection. In samples from gastric patients, the neoplastic tissue showed an accentuated decrease of expression; on the other hand, the expression of E-cadherin was normal in adjacent non-neoplastic.
  
  
  CONCLUSIONS
  No evidence was found of the involvement of the cagA and vacA genes in the decreased expression of E-cadherin and TP53. The process of carcinogenesis is complex, and the decrease of the E-cadherin gene expression and TP53 gene expression appears to contribute significantly.
authors.affiliation: Pró-Reitoria de Pesquisa e Pós-Graduação, Universidade do Sagrado Coração, Rua Irmã Arminda, 10-50, Bauru, 17011160, Brazil.
authors.forename: Mariane Avante
authors.initials: MA
authors.lastname: Ferraz
authors.affiliation: Pró-Reitoria de Pesquisa e Pós-Graduação, Universidade do Sagrado Coração, Rua Irmã Arminda, 10-50, Bauru, 17011160, Brazil.
authors.forename: Luanna Munhoz
authors.initials: LM
authors.lastname: Zabaglia
authors.affiliation: Pró-Reitoria de Pesquisa e Pós-Graduação, Universidade do Sagrado Coração, Rua Irmã Arminda, 10-50, Bauru, 17011160, Brazil.
authors.forename: Weendelly Nayara
authors.initials: WN
authors.lastname: Pereira
authors.affiliation: Pró-Reitoria de Pesquisa e Pós-Graduação, Universidade do Sagrado Coração, Rua Irmã Arminda, 10-50, Bauru, 17011160, Brazil.
authors.forename: Wilson Aparecido
authors.initials: WA
authors.lastname: Orcini
authors.affiliation: Departamento de Genética e Biologia Molecular, Hemocentro, Faculdade de Medicina de Marília, Rua Monte Carmelo, 800, Marília, 17519050, Brazil.
authors.forename: Roger Willian
authors.initials: RW
authors.lastname: de Labio
authors.affiliation: Departamento de Genética e Biologia Molecular, Hemocentro, Faculdade de Medicina de Marília, Rua Monte Carmelo, 800, Marília, 17519050, Brazil.
authors.forename: Agostinho
authors.initials: A
authors.lastname: Caleman Neto
authors.affiliation: Disciplina de Genética, Departamento de Morfologia e Genética, Universidade Federal de São Paulo, Rua Botucatu, 740, São Paulo, 04023900, Brazil.
authors.forename: Fernanda
authors.initials: F
authors.lastname: Wisnieski
authors.affiliation: Disciplina de Genética, Departamento de Morfologia e Genética, Universidade Federal de São Paulo, Rua Botucatu, 740, São Paulo, 04023900, Brazil.
authors.forename: Danielle Queiroz
authors.initials: DQ
authors.lastname: Calcagno
authors.affiliation: Disciplina de Genética, Departamento de Morfologia e Genética, Universidade Federal de São Paulo, Rua Botucatu, 740, São Paulo, 04023900, Brazil.
authors.forename: Leonardo Caires
authors.initials: LC
authors.lastname: Santos
authors.affiliation: Núcleo de Pesquisa em Oncologia, Hospital João de Barros Barreto, Universidade Federal do Pará, Avenida Mundurucus, 4487, Belém, 66073000, Brazil.
authors.forename: Paulo Pimentel
authors.initials: PP
authors.lastname: Assumpção
authors.affiliation: Laboratório de Citogenética Humana, Instituto de Ciências Biológicas, Universidade Federal do Pará, Rua Augusto Correia, 01, Belém, 66075110, Brazil.
authors.forename: Rommel Rodriguez
authors.initials: RR
authors.lastname: Burbano
authors.affiliation: Departamento de Patologia, Universidade Federal de São Paulo, Rua Botucatu, 740, São Paulo, 04023000, Brazil.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Artigiani
authors.affiliation: Disciplina de Genética, Departamento de Morfologia e Genética, Universidade Federal de São Paulo, Rua Botucatu, 740, São Paulo, 04023900, Brazil.
authors.forename: Marilia de Arruda Cardoso
authors.initials: Mde A
authors.lastname: Smith
authors.affiliation: Pró-Reitoria de Pesquisa e Pós-Graduação, Universidade do Sagrado Coração, Rua Irmã Arminda, 10-50, Bauru, 17011160, Brazil.
authors.forename: Spencer Luiz Marques
authors.initials: SL
authors.lastname: Payão
authors.affiliation: Pró-Reitoria de Pesquisa e Pós-Graduação, Universidade do Sagrado Coração, Rua Irmã Arminda, 10-50, Bauru, 17011160, Brazil. lucasrasmussen@gmail.com.
authors.forename: Lucas T
authors.initials: LT
authors.lastname: Rasmussen
mesh_terms: D000328:Adult; D000942:Antigens, Bacterial / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D015415:Biomarkers / Q000032:analysis*; D015820:Cadherins / Q000235:genetics*; D016022:Case-Control Studies; D004279:DNA, Viral / Q000235:genetics; D015536:Down-Regulation; D005260:Female; D005500:Follow-Up Studies; D005753:Gastric Mucosa / Q000378:metabolism / Q000473:pathology; D005756:Gastritis / Q000235:genetics / Q000473:pathology / Q000821:virology; D016481:Helicobacter Infections / Q000235:genetics* / Q000378:metabolism / Q000473:pathology / Q000821:virology; D016480:Helicobacter pylori; D006801:Humans; D008297:Male; D009367:Neoplasm Staging; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology / Q000821:virology; D015996:Survival Rate; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*; D014774:Virulence / Q000235:genetics*
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D015415:Biomarkers; D015820:Cadherins; D004279:DNA, Viral; D012333:RNA, Messenger; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; C074689:VacA protein, Helicobacter pylori; C080866:cagA protein, Helicobacter pylori
keywords: E-cadherin; Gastric cancer; Gene expression; Helicobacter pylori; TP53