title: Deep Sequencing of the T-cell Receptor Repertoire Demonstrates Polyclonal T-cell Infiltrates in Psoriasis.
abstract: It is well known that infiltration of pathogenic T-cells plays an important role in psoriasis pathogenesis. However, the antigen specificity of these activated T-cells is relatively unknown. Previous studies using T-cell receptor polymerase chain reaction technology (TCR-PCR) have suggested there are expanded T-cell receptor (TCR) clones in psoriatic skin, suggesting a response to an unknown psoriatic antigen. Here we describe the results of high-throughput deep sequencing of the entire αβ- and γδ- TCR repertoire in normal healthy skin and psoriatic lesional and non-lesional skin. From this study, we were able to determine that there is a significant increase in the abundance of unique β- and γ- TCR sequences in psoriatic lesional skin compared to non-lesional and normal skin, and that the entire T-cell repertoire in psoriasis is polyclonal, with similar diversity to normal and non-lesional skin. Comparison of the αβ- and γδ- TCR repertoire in paired non-lesional and lesional samples showed many common clones within a patient, and these close were often equally abundant in non-lesional and lesional skin, again suggesting a diverse T-cell repertoire. Although there were similar (and low) amounts of shared β-chain sequences between different patient samples, there was significantly increased sequence sharing of the γ-chain in psoriatic skin from different individuals compared to those without psoriasis. This suggests that although the T-cell response in psoriasis is highly polyclonal, particular γδ- T-cell subsets may be associated with this disease. Overall, our findings present the feasibility of this technology to determine the entire αβ- and γδ- T-cell repertoire in skin, and that psoriasis contains polyclonal and diverse αβ- and γδ- T-cell populations.
authors.affiliation: Laboratory for Investigative Dermatology, The Rockefeller University, New York, NY, USA.
authors.forename: Jamie L
authors.initials: JL
authors.lastname: Harden
authors.affiliation: Adaptive Biotechnologies, Seattle, WA, USA.
authors.forename: David
authors.initials: D
authors.lastname: Hamm
authors.affiliation: Laboratory for Investigative Dermatology, The Rockefeller University, New York, NY, USA.
authors.forename: Nicholas
authors.initials: N
authors.lastname: Gulati
authors.affiliation: Division of Dermatology, Montefiore Medical Center, Albert Einstein College of Medicine, Bronx, NY, USA.
authors.forename: Michelle A
authors.initials: MA
authors.lastname: Lowes
authors.affiliation: Laboratory for Investigative Dermatology, The Rockefeller University, New York, NY, USA.
authors.forename: James G
authors.initials: JG
authors.lastname: Krueger
keywords: T-cell receptor (TCR); T-cell, polyclonal; TCR deep-sequencing, psoriasis; αβ-T-cells; γδ- T-cells