title: Glycine Decarboxylase Expression Increased in p53-Mutated B Cell Lymphoma Mice.
abstract: BACKGROUND
  p53 gene mutations are associated with human tumors, and are among the most common genetic abnormalities. To understand the relationship between p53 mutations and glycine decarboxylase (GLDC) expression in B cell lymphoma, we established B cell lymphoma animal models to study GLDC expression in B cell lymphoma mice.
  
  
  METHODS
  Based on immunohistochemical staining results, BALB/c nude mice were divided into a p53 protein-positive group and a p53 protein-negative group. GLDC mRNA expression was determined by real-time polymerase chain reaction, and GLDC protein expression was determined by Western blot. We designed a GLDC-specific interference fragment siRNA-transfected human B cell lymphoma cell line (Raji) to establish a B cell lymphoma animal model.
  
  
  RESULTS
  The results showed both GLDC mRNA and protein expression increased in the B cell lymphoma tissue of the p53 protein-positive group compared with the p53 protein-negative group. The proliferation ability of GLDC siRNA-transfected cells decreased significantly compared with the negative-control siRNA group and the blank control group (p < 0.05), which showed that the GLDC gene can promote cell proliferation in p53-mutated B cell lymphoma.
  
  
  CONCLUSIONS
  These studies support a direct relationship between p53 mutations and GLDC expression in B cell lymphoma. GLDC can induce dramatic changes in glycolysis and glycine/serine metabolism, leading to changes in pyrimidine metabolism and tumor development.
authors.affiliation: Department of Clinical Laboratory Science, Shandong University Affiliated Qianfoshan Hospital, Jinan, China.
authors.forename: Xiangdong
authors.initials: X
authors.lastname: Li
authors.forename: Chaoyang
authors.initials: C
authors.lastname: Cui
authors.forename: Yuan
authors.initials: Y
authors.lastname: Guo
authors.forename: Guang
authors.initials: G
authors.lastname: Yang
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D050841:Glycine Dehydrogenase (Decarboxylating) / Q000378:metabolism*; D006801:Humans; D016393:Lymphoma, B-Cell / Q000201:enzymology* / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009154:Mutation / Q000235:genetics; D016159:Tumor Suppressor Protein p53 / Q000235:genetics* / Q000378:metabolism*
chemical_list: D016159:Tumor Suppressor Protein p53; D050841:Glycine Dehydrogenase (Decarboxylating)