title: The steroid receptor RNA activator protein (SRAP) controls cancer cell migration/motility.
abstract: The steroid receptor RNA activator gene (SRA1) produces both a functional RNA (SRA) and a protein (SRAP), whose exact physiological roles remain unknown. To identify cellular processes regulated by SRAP we compared the transcriptome of Hela and MDA-MB-231 cancer cells upon depletion of the SRA/SRAP transcripts or overexpression of the SRAP protein. RNA-seq and Ontology analyses pinpointed cellular movement as potentially regulated by SRAP. Using live cell imaging, we found that SRA/SRAP depletion and SRAP overexpression lead respectively to a decrease and increase in cancer cell motility. Our results highlight for the first time a link existing between SRA1 gene expression and cell motility.
authors.affiliation: Manitoba Institute of Cell Biology, 675 McDermot Ave., R3E0V9 Winnipeg, Manitoba, Canada; Department of Biochemistry and Medical Genetics, University of Manitoba, 770 Bannatyne Avenue, R3E0W3 Winnipeg, Manitoba, Canada.
authors.forename: Yi
authors.initials: Y
authors.lastname: Yan
authors.affiliation: Manitoba Institute of Cell Biology, 675 McDermot Ave., R3E0V9 Winnipeg, Manitoba, Canada.
authors.forename: Charlton
authors.initials: C
authors.lastname: Cooper
authors.affiliation: Manitoba Institute of Cell Biology, 675 McDermot Ave., R3E0V9 Winnipeg, Manitoba, Canada; Department of Biochemistry and Medical Genetics, University of Manitoba, 770 Bannatyne Avenue, R3E0W3 Winnipeg, Manitoba, Canada.
authors.forename: Mohammad K
authors.initials: MK
authors.lastname: Hamedani
authors.affiliation: Manitoba Institute of Cell Biology, 675 McDermot Ave., R3E0V9 Winnipeg, Manitoba, Canada; Department of Biochemistry and Medical Genetics, University of Manitoba, 770 Bannatyne Avenue, R3E0W3 Winnipeg, Manitoba, Canada.
authors.forename: Brent
authors.initials: B
authors.lastname: Guppy
authors.affiliation: Manitoba Institute of Cell Biology, 675 McDermot Ave., R3E0V9 Winnipeg, Manitoba, Canada.
authors.forename: Wayne
authors.initials: W
authors.lastname: Xu
authors.affiliation: Manitoba Institute of Cell Biology, 675 McDermot Ave., R3E0V9 Winnipeg, Manitoba, Canada.
authors.forename: Deborah
authors.initials: D
authors.lastname: Tsuyuki
authors.affiliation: Department of Immunology, University of Manitoba, 413 Apotex Center, 750 McDermot Ave., R3E0T5 Winnipeg, Manitoba, Canada.
authors.forename: Christine
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Manitoba Institute of Cell Biology, 675 McDermot Ave., R3E0V9 Winnipeg, Manitoba, Canada.
authors.forename: Zoann
authors.initials: Z
authors.lastname: Nugent
authors.affiliation: Manitoba Institute of Cell Biology, 675 McDermot Ave., R3E0V9 Winnipeg, Manitoba, Canada; Department of Physiology, University of Manitoba, 770 Bannatyne Avenue, R3E0W3 Winnipeg, Manitoba, Canada.
authors.forename: Anne
authors.initials: A
authors.lastname: Blanchard
authors.affiliation: Manitoba Institute of Cell Biology, 675 McDermot Ave., R3E0V9 Winnipeg, Manitoba, Canada; Department of Biochemistry and Medical Genetics, University of Manitoba, 770 Bannatyne Avenue, R3E0W3 Winnipeg, Manitoba, Canada.
authors.forename: James R
authors.initials: JR
authors.lastname: Davie
authors.affiliation: Manitoba Institute of Cell Biology, 675 McDermot Ave., R3E0V9 Winnipeg, Manitoba, Canada; Department of Biochemistry and Medical Genetics, University of Manitoba, 770 Bannatyne Avenue, R3E0W3 Winnipeg, Manitoba, Canada.
authors.forename: Kirk
authors.initials: K
authors.lastname: McManus
authors.affiliation: Manitoba Institute of Cell Biology, 675 McDermot Ave., R3E0V9 Winnipeg, Manitoba, Canada; Department of Biochemistry and Medical Genetics, University of Manitoba, 770 Bannatyne Avenue, R3E0W3 Winnipeg, Manitoba, Canada.
authors.forename: Leigh C
authors.initials: LC
authors.lastname: Murphy
authors.affiliation: Manitoba Institute of Cell Biology, 675 McDermot Ave., R3E0V9 Winnipeg, Manitoba, Canada; Department of Physiology, University of Manitoba, 770 Bannatyne Avenue, R3E0W3 Winnipeg, Manitoba, Canada.
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Myal
authors.affiliation: Manitoba Institute of Cell Biology, 675 McDermot Ave., R3E0V9 Winnipeg, Manitoba, Canada; Department of Biochemistry and Medical Genetics, University of Manitoba, 770 Bannatyne Avenue, R3E0W3 Winnipeg, Manitoba, Canada. Electronic address: etienne.leygue@umanitoba.ca.
authors.forename: Etienne
authors.initials: E
authors.lastname: Leygue
mesh_terms: D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology; D002352:Carrier Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D002465:Cell Movement; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D018715:Microscopy, Video; D009361:Neoplasm Invasiveness; D009363:Neoplasm Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D012334:RNA, Neoplasm / Q000037:antagonists & inhibitors / Q000378:metabolism*; D034741:RNA, Small Interfering; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism; D059010:Single-Cell Analysis; D002583:Uterine Cervical Neoplasms / Q000378:metabolism* / Q000473:pathology
chemical_list: D002352:Carrier Proteins; D008164:Luminescent Proteins; D009363:Neoplasm Proteins; D012334:RNA, Neoplasm; D034741:RNA, Small Interfering; D011993:Recombinant Fusion Proteins; C548898:steroid receptor RNA activator, human
keywords: Cancer; Cell motility; Functional RNA; Non-coding RNA; Steroid receptor RNA activator; Steroid receptor RNA protein