title: Sensitive and label-free detection of miRNA-145 by triplex formation.
abstract: The development of new strategies for detecting microRNAs (miRNAs) has become a crucial step in the diagnostic field. miRNA profiles depend greatly on the sample and the analytical platform employed, leading sometimes to contradictory results. In this work, we study the use of modified parallel tail-clamps to detect a miRNA sequence involved in tumor suppression by triplex formation. Thermal denaturing curves and circular dichroism (CD) measurements have been performed to confirm that parallel clamps carrying 8-aminoguanine form the most stable triplex structures with their target miRNA. The modified tail-clamps have been tested as bioreceptors in a surface plasmon resonance (SPR) biosensor for the detection of miRNA-145. The detection limit was improved 2.4 times demonstrating that a stable triplex structure is formed between target miRNA and 8-aminoguanine tail-clamp bioreceptor. This new approach is an essential step toward the label-free and reliable detection of miRNA signatures for diagnostic purposes.
authors.affiliation: Department of Chemical and Biomolecular Nanotechnology, Institute for Advanced Chemistry of Catalonia (IQAC), CSIC, c/Jordi Girona 18-26, 08034, Barcelona, Spain.|CIBER-BBN Networking Center on Bioengineering, Biomaterials and Nanomedicine, c/ Jordi Girona 18-26, 08034, Barcelona, Spain.
authors.forename: Anna
authors.initials: A
authors.lastname: Aviñó
authors.affiliation: Catalan Institute of Nanoscience and Nanotechnology (ICN2), CSIC, Edificio ICN2 Building, Campus UAB, Bellaterra, 08193, Barcelona, Spain.|CIBER-BBN Networking Center on Bioengineering, Biomaterials and Nanomedicine, c/ Jordi Girona 18-26, 08034, Barcelona, Spain.
authors.forename: César S
authors.initials: CS
authors.lastname: Huertas
authors.affiliation: Catalan Institute of Nanoscience and Nanotechnology (ICN2), CSIC, Edificio ICN2 Building, Campus UAB, Bellaterra, 08193, Barcelona, Spain.|CIBER-BBN Networking Center on Bioengineering, Biomaterials and Nanomedicine, c/ Jordi Girona 18-26, 08034, Barcelona, Spain.
authors.forename: Laura M
authors.initials: LM
authors.lastname: Lechuga
authors.affiliation: Department of Chemical and Biomolecular Nanotechnology, Institute for Advanced Chemistry of Catalonia (IQAC), CSIC, c/Jordi Girona 18-26, 08034, Barcelona, Spain. recgma@cid.csic.es.|CIBER-BBN Networking Center on Bioengineering, Biomaterials and Nanomedicine, c/ Jordi Girona 18-26, 08034, Barcelona, Spain. recgma@cid.csic.es.
authors.forename: Ramon
authors.initials: R
authors.lastname: Eritja
mesh_terms: D004247:DNA / Q000737:chemistry*; D006801:Humans; D035683:MicroRNAs / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D012680:Sensitivity and Specificity; D020349:Surface Plasmon Resonance / Q000379:methods*
chemical_list: C541010:MIRN145 microRNA, human; D035683:MicroRNAs; C051802:triplex DNA; D004247:DNA
keywords: 8-Amino-2′-deoxyguanosine; Parallel tail-clamp; SPR biosensor; Target miRNA-145; Triplex