title: Structural characterization and immunomodulating activity of polysaccharide from Dendrobium officinale.
abstract: A neutral heteropolysaccharide (DOP-1-1) consisted by mannose and glucose (5.9:1) with an average molecular weight at about 1.78×10(5) Da was purified from Dendrobium officinale. Based on Fourier transform infrared spectrum (FT-IR) and nuclear magnetic resonance (NMR) spectra, it suggested that partial structure of DOP-1-1 is an O-acetylated glucomannan with β-d configuration in pyranose sugar forms. The immunomodulatory activity of DOP-1-1 was evaluated by secretion level of cytokine (interleukin (IL)-1β and IL-10) and tumor necrosis factor (TNF)-α in vitro. Our results suggested that DOP-1-1 could stimulate cytokine production (TNF-α, IL-1β) in cells. These findings demonstrated that the purified polysaccharide from D. officinale presented significant immune-modulating activities. Furthermore, by Western-blot we can found that the signaling pathways of DOP-1-1 induced immune activities involving ERK1/2 and NF-кB. As to antioxidant activity, DOP-1-1 hadn't showed remarkable scavenging capacity of 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) in contrast with other studies of polysaccharides from D. officinale.
authors.affiliation: College of Food Science and Technology, Yunna Agricultural University, Kunming 650201, China; Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China; Key Laboratory of Pu-er Tea Science, Ministry of Education, Yunnan Agricultural University, Kunming 650201, China.
authors.forename: Tao-Bin
authors.initials: TB
authors.lastname: He
authors.affiliation: College of Food Science and Technology, Yunna Agricultural University, Kunming 650201, China; Key Laboratory of Pu-er Tea Science, Ministry of Education, Yunnan Agricultural University, Kunming 650201, China.
authors.forename: Yan-Ping
authors.initials: YP
authors.lastname: Huang
authors.affiliation: Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China.
authors.forename: Liu
authors.initials: L
authors.lastname: Yang
authors.affiliation: College of Food Science and Technology, Yunna Agricultural University, Kunming 650201, China; Key Laboratory of Pu-er Tea Science, Ministry of Education, Yunnan Agricultural University, Kunming 650201, China.
authors.forename: Ti-Ti
authors.initials: TT
authors.lastname: Liu
authors.affiliation: College of Food Science and Technology, Yunna Agricultural University, Kunming 650201, China; Key Laboratory of Pu-er Tea Science, Ministry of Education, Yunnan Agricultural University, Kunming 650201, China.
authors.forename: Wan-Ying
authors.initials: WY
authors.lastname: Gong
authors.affiliation: College of Food Science and Technology, Yunna Agricultural University, Kunming 650201, China; Key Laboratory of Pu-er Tea Science, Ministry of Education, Yunnan Agricultural University, Kunming 650201, China.
authors.forename: Xuan-Jun
authors.initials: XJ
authors.lastname: Wang
authors.affiliation: College of Food Science and Technology, Yunna Agricultural University, Kunming 650201, China; Key Laboratory of Pu-er Tea Science, Ministry of Education, Yunnan Agricultural University, Kunming 650201, China. Electronic address: shengjunpuer@163.com.
authors.forename: Jun
authors.initials: J
authors.lastname: Sheng
authors.affiliation: Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China. Electronic address: hujiangmiao@mail.kib.ac.cn.
authors.forename: Jiang-Miao
authors.initials: JM
authors.lastname: Hu
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D001713:Biphenyl Compounds / Q000494:pharmacology; D002478:Cells, Cultured; D031667:Dendrobium / Q000737:chemistry*; D006801:Humans; D056747:Immunomodulation / Q000187:drug effects*; D016753:Interleukin-10 / Q000378:metabolism; D053583:Interleukin-1beta / Q000378:metabolism; D008358:Mannose / Q000378:metabolism; D010851:Picrates / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology*; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D053583:Interleukin-1beta; D010851:Picrates; D010936:Plant Extracts; D011134:Polysaccharides; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; C004931:1,1-diphenyl-2-picrylhydrazyl; D008358:Mannose
keywords: Dendrobium officinale; Immunomodulation; Polysaccharide