title: Bacterial colonization status of cystic fibrosis children's toothbrushes: A pilot study.
abstract: BACKGROUND
  Pseudomonas aeruginosa and Staphylococcus aureus toothbrush contamination in cystic fibrosis (CF) is unknown. This pilot study aimed to determine their prevalence and the potential involvement of toothbrushes in pulmonary infection.
  
  
  METHODS
  Toothbrush bacteriological analysis for children aged 8-18 years was conducted on 27 CF patients, 15 healthy siblings, and 15 healthy children from the general population.
  
  
  RESULTS
  S. aureus was detected on 22% of the patients' toothbrushes, and 13% of healthy children's toothbrushes and P. aeruginosa on 15% of patients' toothbrushes and 0-13% of healthy children's toothbrushes. There was no statistical correlation between pulmonary colonization and toothbrush contamination. P. aeruginosa genotyping showed two identical clones on the patients' toothbrushes and in their sputum, and between one patient's sputum and his sibling's toothbrush.
  
  
  CONCLUSIONS
  S. aureus and P. aeruginosa can colonize CF patients' toothbrushes. The impact on pulmonary colonization remains unknown. Toothbrush decontamination methods need to consider these bacteria in CF patients.
authors.affiliation: CRCM pédiatrique, service de pneumologie, hôpital des Enfants, 330, avenue de Grande-Bretagne, TSA 70034, 31059 Toulouse cedex 9, France.
authors.forename: A
authors.initials: A
authors.lastname: Genevois
authors.affiliation: Laboratoire de génie chimique, UMR5503, faculté des sciences pharmaceutiques, université Toulouse III, 35, chemin des Maraîchers, 31062 Toulouse cedex 4, France.
authors.forename: C
authors.initials: C
authors.lastname: Roques
authors.affiliation: Laboratoire de bactériologie-hygiène, pôle biologie, institut fédératif de biologie (IFB), 330, avenue de Grande-Bretagne, TSA 40031, 31059 Toulouse cedex 9, France.
authors.forename: C
authors.initials: C
authors.lastname: Segonds
authors.affiliation: Laboratoire de bactériologie-hygiène, pôle biologie, institut fédératif de biologie (IFB), 330, avenue de Grande-Bretagne, TSA 40031, 31059 Toulouse cedex 9, France.
authors.forename: L
authors.initials: L
authors.lastname: Cavalié
authors.affiliation: CRCM pédiatrique, service de pneumologie, hôpital des Enfants, 330, avenue de Grande-Bretagne, TSA 70034, 31059 Toulouse cedex 9, France.
authors.forename: F
authors.initials: F
authors.lastname: Brémont
authors.affiliation: Centre de recherche en management, UMR CNRS 5303, IAE de l'université Toulouse I, 2, rue du Doyen Gabriel-Marty, 31042 Toulouse cedex 9, France; Université François-Rabelais de Tours, université d'Orléans, Vallorem EA6296, 50, avenue Jean-Portalis, 37200 Tours, France.
authors.forename: L
authors.initials: L
authors.lastname: Maubisson
authors.affiliation: CRCM pédiatrique, service de gastroentérologie-hépatologie, hôpital des enfants, 330, avenue de Grande-Bretagne, TSA 70034, 31059 Toulouse cedex 9, France.
authors.forename: E
authors.initials: E
authors.lastname: Mas
authors.affiliation: CRCM pédiatrique, service de pneumologie, hôpital des Enfants, 330, avenue de Grande-Bretagne, TSA 70034, 31059 Toulouse cedex 9, France. Electronic address: mittaine.m@chu-toulouse.fr.
authors.forename: M
authors.initials: M
authors.lastname: Mittaine
mesh_terms: D000293:Adolescent; D002648:Child; D003550:Cystic Fibrosis; D003742:Dental Devices, Home Care / Q000382:microbiology*; D004866:Equipment Contamination; D005260:Female; D006801:Humans; D008168:Lung / Q000382:microbiology; D008297:Male; D010865:Pilot Projects; D011550:Pseudomonas aeruginosa / Q000302:isolation & purification*; D013183:Sputum / Q000382:microbiology; D013211:Staphylococcus aureus / Q000302:isolation & purification*