title: [Effect of aralosides to contraction function and calcium transient of ischemia/reperfusion myocardial cells].
abstract: To discuss the protective effect of aralosides (AS) on I/R-induced rat myocardial injury. The adult rat ventricular myocyte ischemia model was established through perfusion with sodium lactate perfusate and reperfusion with Ca(2+) -containing Tyrode's solution simulation. The cell contraction and ion concentration synchronization determination system was applied to detect the effect of AS on single I/R cell contraction and Ca2+ transients. According to the findings, AS could increase resting sarcomere length, contraction amplitude, ± dL/dt(max), calcium transient amplitude and speed of post-reperfusion myocardial cells (P < 0.05, P < 0.01), and decrease in time for achieving 90.0% of maximum relaxation, time for achieving peak value, resting calcium ratio, contraction period [Ca2+] i, time for achieving 50.0% of maximum relaxation and attenuation rate of intracellular calcium transient (P < 0.05, P < 0.01). Therefore, it is suggested that AS improved the post-reperfusion cell contraction and injury of calcium homeostasis.
authors.forename: Miao-di
authors.initials: MD
authors.lastname: Zhang
authors.forename: Gui-bo
authors.initials: GB
authors.lastname: Sun
authors.forename: Hui-bo
authors.initials: HB
authors.lastname: Xu
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.forename: Xiao-bo
authors.initials: XB
authors.lastname: Sun
mesh_terms: D000818:Animals; D027983:Aralia / Q000737:chemistry*; D001692:Biological Transport / Q000187:drug effects; D002118:Calcium / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D006801:Humans; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D017202:Myocardial Ischemia / Q000188:drug therapy* / Q000378:metabolism / Q000503:physiopathology / Q000601:surgery; D015425:Myocardial Reperfusion; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012503:Saponins / Q000008:administration & dosage*
chemical_list: D004365:Drugs, Chinese Herbal; D012503:Saponins; D002118:Calcium