title: Systematic analyses of the ultraviolet radiation resistance-associated gene product (UVRAG) protein interactome by tandem affinity purification.
abstract: Ultraviolet radiation resistance-associated gene product (UVRAG) was originally identified as a protein involved in cellular responses to UV irradiation. Subsequent studies have demonstrated that UVRAG plays as an important role in autophagy, a lysosome-dependent catabolic program, as a part of a pro-autophagy PIK3C3/VPS34 lipid kinase complex. Several recent studies have shown that UVRAG is also involved in autophagy-independent cellular functions, such as DNA repair/stability and vesicular trafficking/fusion. Here, we examined the UVRAG protein interactome to obtain information about its functional network. To this end, we screened UVRAG-interacting proteins using a tandem affinity purification method coupled with MALDI-TOF/MS analysis. Our results demonstrate that UVRAG interacts with various proteins involved in a wide spectrum of cellular functions, including genome stability, protein translational elongation, protein localization (trafficking), vacuole organization, transmembrane transport as well as autophagy. Notably, the interactome list of high-confidence UVRAG-interacting proteins is enriched for proteins involved in the regulation of genome stability. Our systematic UVRAG interactome analysis should provide important clues for understanding a variety of UVRAG functions.
authors.affiliation: Department of Oral Biochemistry and Molecular Biology, School of Dentistry, Research Center for Tooth and Periodontal Tissue Regeneration, Kyung Hee University, Seoul, 130-701, Korea.
authors.forename: Ji-Hye
authors.initials: JH
authors.lastname: Son
authors.affiliation: Department of Chemistry, New York University, New York, NY, 10003, USA.
authors.forename: Eurim C
authors.initials: EC
authors.lastname: Hwang
authors.affiliation: Department of Oral Biochemistry and Molecular Biology, School of Dentistry, Research Center for Tooth and Periodontal Tissue Regeneration, Kyung Hee University, Seoul, 130-701, Korea. jok011@khu.ac.kr.
authors.forename: Joungmok
authors.initials: J
authors.lastname: Kim
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005047:Etoposide / Q000494:pharmacology; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D000072200:Ku Autoantigen / Q000378:metabolism; D051379:Mice; D011485:Protein Binding / Q000187:drug effects; D025941:Protein Interaction Mapping / Q000379:methods*; D011506:Proteins / Q000302:isolation & purification* / Q000378:metabolism*; D025521:Tumor Suppressor Proteins / Q000378:metabolism*
chemical_list: D011506:Proteins; D025521:Tumor Suppressor Proteins; C512859:UVRAG protein, mouse; D005047:Etoposide; C000606512:Xrcc5 protein, mouse; D000072200:Ku Autoantigen
keywords: Interactome analysis; Tandem affinity purification; UVRAG