title: Phosphoprotein Detection by High-Throughput Flow Cytometry.
abstract: Phospho flow cytometry is a powerful technique for the detection of protein phosphorylation events that, like Western blotting, relies on phospho-epitope-specific antibodies. In contrast to the latter, however, multidimensional and directly quantifiable data is obtained at the single-cell level allowing separate analysis of small cell populations in complex cellular mixtures. Furthermore, up to 30 phospho-specific antibodies or antibodies identifying other posttranslational modifications in combination with cell surface markers can be analyzed in a single experiment. Utilizing a technique called fluorescent cell barcoding that enables combination of up to 64 samples into one tube for multiplex analysis and later data deconvolution, phospho flow cytometry is turned into a medium- to high-throughput technology.
authors.affiliation: Biotechnology Centre, University of Oslo, Oslo, 0318, Norway.|K.G. Jebsen Centre for Cancer Immunotherapy, University of Oslo, Oslo, 0318, Norway.|Centre for Molecular Medicine Norway, Nordic EMBL Partnership, University of Oslo, Oslo, 0318, Norway.
authors.forename: Johannes
authors.initials: J
authors.lastname: Landskron
authors.affiliation: Biotechnology Centre, University of Oslo, Oslo, 0318, Norway. kjetil.tasken@ncmm.uio.no.|K.G. Jebsen Centre for Cancer Immunotherapy, University of Oslo, Oslo, 0318, Norway. kjetil.tasken@ncmm.uio.no.|Centre for Molecular Medicine Norway, Nordic EMBL Partnership, University of Oslo, Oslo, 0318, Norway. kjetil.tasken@ncmm.uio.no.|K.G. Jebsen Inflammation Research Centre, University of Oslo, Oslo, 0317, Norway. kjetil.tasken@ncmm.uio.no.|Department of Infectious Diseases, University of Oslo, 0407, Oslo, Norway. kjetil.tasken@ncmm.uio.no.
authors.forename: Kjetil
authors.initials: K
authors.lastname: Taskén
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D000918:Antibody Specificity; D000939:Epitopes; D005434:Flow Cytometry; D005456:Fluorescent Dyes / Q000737:chemistry; D057166:High-Throughput Screening Assays; D006801:Humans; D008163:Luminescent Measurements; D010750:Phosphoproteins / Q000032:analysis* / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational; D040901:Proteomics / Q000379:methods*; D057188:Workflow
chemical_list: D000906:Antibodies; D000939:Epitopes; D005456:Fluorescent Dyes; D010750:Phosphoproteins
keywords: Flow cytometry; Fluorescent cell barcoding; Phospho flow; Phospho-specific antibody; Signaling