title: Stability of aspartame and neotame in pasteurized and in-bottle sterilized flavoured milk.
abstract: Analytical high performance liquid chromatography (HPLC) conditions were standardized along with the isolation procedure for separation of aspartame and neotame in flavoured milk (pasteurized and in-bottle sterilized flavoured milk). The recovery of the method was approximately 98% for both aspartame and neotame. The proposed HPLC method can be successfully used for the routine determination of aspartame and neotame in flavoured milk. Pasteurization (90 °C/20 min) resulted in approximately 40% loss of aspartame and only 8% of neotame was degraded. On storage (4-7°C/7 days) aspartame and neotame content decreased significantly (P<0.05) from 59.70% to 44.61% and 91.78% to 87.18%, respectively. Sterilization (121 °C/15 min) resulted in complete degradation of aspartame; however, 50.50% of neotame remained intact. During storage (30 °C/60 days) neotame content decreased significantly (P<0.05) from 50.36% to 8.67%. Results indicated that neotame exhibited better stability than aspartame in both pasteurized and in-bottle sterilized flavoured milk.
authors.affiliation: Dairy Chemistry Division, National Dairy Research Institute, Karnal, Haryana 132001, India. Electronic address: anu.ndri@gmail.com.
authors.forename: Anuradha
authors.initials: A
authors.lastname: Kumari
authors.affiliation: Dairy Chemistry Division, National Dairy Research Institute, Karnal, Haryana 132001, India. Electronic address: sonikachoudhary15@gmail.com.
authors.forename: Sonika
authors.initials: S
authors.lastname: Choudhary
authors.affiliation: Dairy Chemistry Division, National Dairy Research Institute, Karnal, Haryana 132001, India. Electronic address: sumitak123@gmail.com.
authors.forename: Sumit
authors.initials: S
authors.lastname: Arora
authors.affiliation: Dairy Chemistry Division, National Dairy Research Institute, Karnal, Haryana 132001, India. Electronic address: vishk12000@yahoo.com.
authors.forename: Vivek
authors.initials: V
authors.lastname: Sharma
mesh_terms: D000818:Animals; D001218:Aspartame / Q000737:chemistry*; D004151:Dipeptides / Q000737:chemistry*; D004355:Drug Stability; D005421:Flavoring Agents / Q000032:analysis; D061353:Food Storage; D006863:Hydrogen-Ion Concentration; D008892:Milk / Q000737:chemistry*; D061352:Pasteurization; D013242:Sterilization
chemical_list: D004151:Dipeptides; D005421:Flavoring Agents; C404525:neotame; D001218:Aspartame
keywords: Aspartame; Comparative stability; In-bottle sterilized flavoured milk; Neotame; Pasteurized flavoured milk