title: Effect of acetone extract from stem bark of Acacia species (A. dealbata, A. ferruginea and A. leucophloea) on antioxidant enzymes status in hydrogen peroxide-induced HepG2 cells.
abstract: Acacia species are multipurpose trees, widely used in the traditional systems of medicine to treat various ailments. The major objective of the present study was to determine the gene expression of enzymatic antioxidants by acetone extract from the stem bark of three Acacia species (Acacia dealbata, Acacia ferruginea and Acacia leucophloea) in hydrogen peroxide (H2O2)-induced human hepatoma (HepG2) cells. The expression of antioxidant enzymes such as superoxide dismutase containing copper-zinc (CuZnSOD)/manganese (MnSOD), catalase (CAT) and glutathione peroxidase (GPx) in HepG2 cells was evaluated by real-time PCR. The results of antioxidant enzyme expression in real-time PCR study revealed that the H2O2 (200 μM) challenged HepG2 cells reduced the expression of enzymes such as SOD, GPx and CAT. However, the cells pre-treated with acetone extracts of all the three Acacia species significantly (P > 0.05) up-regulated the expression of antioxidant enzymes in a concentration dependent manner (25, 50 and 75 μg/mL). In conclusion, the findings of our study demonstrated that the acetone extract of Acacia species effectively inhibited H2O2 mediated oxidative stress and may be useful as a therapeutic agent in preventing oxidative stress mediated diseases.
authors.affiliation: Department of Process and Food Engineering, Faculty of Engineering, Universiti Putra Malaysia, Serdang, Selangor 43400, Malaysia.
authors.forename: Kandhasamy
authors.initials: K
authors.lastname: Sowndhararajan
authors.affiliation: Department of Animal Products and Food Science, Kangwon National University, Chuncheon, Gangwon 200-701, Republic of Korea.
authors.forename: Sunghyun
authors.initials: S
authors.lastname: Hong
authors.affiliation: Department of Animal Products and Food Science, Kangwon National University, Chuncheon, Gangwon 200-701, Republic of Korea.
authors.forename: Jin-Woo
authors.initials: JW
authors.lastname: Jhoo
authors.affiliation: Department of Biological Environment, Kangwon National University, Chuncheon, Gangwon 200-701, Republic of Korea.
authors.forename: Songmun
authors.initials: S
authors.lastname: Kim
authors.affiliation: Department of Process and Food Engineering, Faculty of Engineering, Universiti Putra Malaysia, Serdang, Selangor 43400, Malaysia.
authors.forename: Nyuk Ling
authors.initials: NL
authors.lastname: Chin
keywords: Acacia; Antioxidant enzyme; Catalase; HepG2 cells; Superoxide dismutase