title: Functional diversity and dynamics of bacterial communities in a membrane bioreactor for the treatment of metal-working fluid wastewater.
abstract: An extensive microbiological study has been carried out in a membrane bioreactor fed with activated sludge and metal-working fluids. Functional diversity and dynamics of bacterial communities were studied with different approaches. Functional diversity of culturable bacterial communities was studied with different Biolog™ plates. Structure and dynamics of bacterial communities were studied in culturable and in non-culturable fractions using a 16S rRNA analysis. Among the culturable bacteria, Alphaproteobacteria and Gammaproteobacteria were the predominant classes. However, changes in microbial community structure were detected over time. Culture-independent analysis showed that Betaproteobacteria was the most frequently detected class in the membrane bioreactor (MBR) community with Zoogloea and Acidovorax as dominant genera. Also, among non-culturable bacteria, a process of succession was observed. Longitudinal structural shifts observed were more marked for non-culturable than for culturable bacteria, pointing towards an important role in the MBR performance. Microbial community metabolic abilities assessed with Biolog™ Gram negative, Gram positive and anaerobic plates also showed differences over time for Shannon's diversity index, kinetics of average well colour development, and the intensely used substrates by bacterial community in each plate.
authors.affiliation: Department of Pharmaceutical and Health Sciences, Facultad Farmacia, Universidad San Pablo CEU., Urb. Monteprincipe, Boadilla del Monte, 28668 Madrid, Spain E-mail: alucgar@ceu.es.
authors.forename: Lucía
authors.initials: L
authors.lastname: Grijalbo
authors.affiliation: Department of Ecology and Natural Resources, Soil Microbial Ecology Group, NEIKER-Tecnalia, c/Berreaga 1, E-48160 Derio, Spain.
authors.forename: Carlos
authors.initials: C
authors.lastname: Garbisu
authors.affiliation: Department of Ecology and Natural Resources, Soil Microbial Ecology Group, NEIKER-Tecnalia, c/Berreaga 1, E-48160 Derio, Spain.
authors.forename: Iker
authors.initials: I
authors.lastname: Martín
authors.affiliation: GAIKER Tecnological Centre, IK4 Research Alliance, E-48170 Zamudio, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: Etxebarria
authors.affiliation: Department of Pharmaceutical and Health Sciences, Facultad Farmacia, Universidad San Pablo CEU., Urb. Monteprincipe, Boadilla del Monte, 28668 Madrid, Spain E-mail: alucgar@ceu.es.
authors.forename: F Javier
authors.initials: FJ
authors.lastname: Gutierrez-Mañero
authors.affiliation: Department of Pharmaceutical and Health Sciences, Facultad Farmacia, Universidad San Pablo CEU., Urb. Monteprincipe, Boadilla del Monte, 28668 Madrid, Spain.
authors.forename: Jose Antonio
authors.initials: JA
authors.lastname: Lucas Garcia
mesh_terms: D001419:Bacteria / Q000145:classification*; D019149:Bioreactors / Q000382:microbiology*; D004269:DNA, Bacterial / Q000032:analysis; D008669:Metallurgy; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D062065:Wastewater / Q000382:microbiology*; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D062065:Wastewater