title: Novel analytical procedure using a combination of hollow fiber supported liquid membrane and dispersive liquid-liquid microextraction for the determination of aflatoxins in soybean juice by high performance liquid chromatography - Fluorescence detector.
abstract: This study describes a combination between hollow fiber membrane and dispersive liquid-liquid microextraction for determination of aflatoxins in soybean juice by HPLC. The main advantage of this approach is the use of non-chlorinated solvent and small amounts of organic solvents. The optimum extraction conditions were 1-octanol as immobilized solvent; toluene and acetone at 1:5 ratio as extraction and disperser solvents (100 μL), NaCl at 2% of the sample volume and extraction time of 60 min. The optimal condition for the liquid desorption was 150 μL acetonitrile:water (50:50 v/v) and desorption time of 20 min. The linear range varied from 0.03 to 21 μg L(-1), with R(2) coefficients ranging from 0.9940 to 0.9995. The limits of detection and quantification ranged from 0.01 μg L(-1) to 0.03 μg L(-1) and from 0.03 μg L(-1) to 0.1 μg L(-1), respectively. Recovery tests ranged from 72% to 117% and accuracy between 12% and 18%.
authors.affiliation: Departamento de Química, Universidade Federal de Santa Catarina, Florianópolis, Santa Catarina, Brazil.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Simão
authors.affiliation: Departamento de Química, Universidade Federal de Santa Catarina, Florianópolis, Santa Catarina, Brazil.
authors.forename: Josias
authors.initials: J
authors.lastname: Merib
authors.affiliation: Departamento de Química, Universidade Federal de Santa Catarina, Florianópolis, Santa Catarina, Brazil.
authors.forename: Adriana N
authors.initials: AN
authors.lastname: Dias
authors.affiliation: Departamento de Química, Universidade Federal de Santa Catarina, Florianópolis, Santa Catarina, Brazil. Electronic address: eduardo.carasek@ufsc.br.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Carasek
mesh_terms: D000348:Aflatoxins / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005453:Fluorescence; D059627:Liquid Phase Microextraction / Q000379:methods*; D013025:Glycine max / Q000737:chemistry*
chemical_list: D000348:Aflatoxins
keywords: Aflatoxins; Dispersive liquid–liquid microextraction; Hollow fiber supported liquid membrane; Sample preparation; Soybean juice