title: Analyses of a satiety factor NUCB2/nesfatin-1; gene expressions and modulation by different dietary components in dogs.
abstract: Nesfatin-1 is an anorexic peptide derived from a precursor, nucleobindin-2 (NUCB2), which is distributed in various organs, coexists with ghrelin in the gastric X/A-like cells and closely relates to an appetite control in rodents and humans. Nesfatin-1 may be a significant factor addressing the satiety also in veterinary medicine, however, there are few reports about nesfatin-1 in dogs. In the present study, we detected canine NUCB2/nesfatin-1 mRNA in various tissues, especially abundant in pancreas, gastrointestinal tracts, testis and cerebellum. We examined circulating nesfatin-1 concentrations and NUCB2/nesfatin-1 mRNA expressions in upper gastrointestinal tracts (gastric corpus, pyloric antrum and duodenum) in dogs fed on different types of diets. Plasma nesfatin-1 concentrations in the dogs were approximately 4 ng/ml and they did not change after feeding through the study, however, NUCB2/nesfatin-1 mRNA expressions in pyloric antrum were 1.84-fold higher in the dogs fed on a High fiber/High protein diet (P<0.001), 1.48-fold higher in the dogs fed on a High fat/Low protein diet (P<0.05) and 1.02-fold higher in the dogs fed on a Low fat/High carbohydrate diet (not significant) comparing to those on a control diet. It was concluded that High fiber/High protein and High fat/Low protein diets increased NUCB2/nesfatin-1 production in canine gastrointestinal tracts. These results may set the stage for further investigations of canine NUCB2/nesfatin-1, which may relate to satiety effects in dogs.
authors.affiliation: Department of Veterinary Nursing, School of Veterinary Nursing and Technology, Faculty of Veterinary Science, Nippon Veterinary and Life Science University, 1-7-1 Kyonan-cho, Musashino-shi, Tokyo 180-8602, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Nozawa
authors.forename: Tomoko
authors.initials: T
authors.lastname: Kimura
authors.forename: Miyuki
authors.initials: M
authors.lastname: Kurishima
authors.forename: Kana
authors.initials: K
authors.lastname: Mimura
authors.forename: Kaori
authors.initials: K
authors.lastname: Saeki
authors.forename: Yohei
authors.initials: Y
authors.lastname: Miki
authors.forename: Hitomi
authors.initials: H
authors.lastname: Oda
authors.forename: Akihiro
authors.initials: A
authors.lastname: Mori
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Momota
authors.forename: Daigo
authors.initials: D
authors.lastname: Azakami
authors.forename: Katsumi
authors.initials: K
authors.lastname: Ishioka
mesh_terms: D000818:Animals; D002135:Calcium-Binding Proteins / Q000097:blood / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000097:blood / Q000235:genetics*; D004032:Diet; D004285:Dogs / Q000235:genetics*; D005753:Gastric Mucosa / Q000378:metabolism; D041981:Gastrointestinal Tract / Q000378:metabolism; D015870:Gene Expression; D009419:Nerve Tissue Proteins / Q000097:blood / Q000235:genetics*; D000080866:Nucleobindins; D010179:Pancreas / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D012528:Satiety Response
chemical_list: D002135:Calcium-Binding Proteins; D004268:DNA-Binding Proteins; C000630650:NUCB2 protein, human; D009419:Nerve Tissue Proteins; D000080866:Nucleobindins; D012333:RNA, Messenger