title: Hsa_circ_0001649: A circular RNA and potential novel biomarker for hepatocellular carcinoma.
abstract: BACKGROUND
  It has been shown that circular RNA (circRNA) is associated with human cancers, however, few studies have been reported in hepatocellular carcinoma (HCC).
  
  
  OBJECTIVE
  To estimate clinical values of a circular RNA, Hsa_circ_0001649, in HCC.
  
  
  METHODS
  Expression level of hsa_circ_0001649 was detected in HCC and paired adjacent liver tissues by real-time quantitative reverse transcription-polymerase chain reactions (qRT-PCRs). Differences in expression level of hsa_circ_0001649 were analyzed using the paired t-test. Tests were performed between clinical information and hsa_circ_0001649 expression level by analysis of variance (ANOVA) or welch t-test and a receiver operating characteristics (ROC) curve was established to estimate the value of hsa_circ_0001649 expression as a biomarker in HCC.
  
  
  RESULTS
  hsa_circ_0001649 expression was significantly downregulated in HCC tissues (p = 0.0014) based on an analysis of 89 paired samples of HCC and adjacent liver tissues and the area under the ROC curve (AUC) was 0.63. Furthermore, hsa_circ_0001649 expression was correlated with tumor size (p = 0.045) and the occurrence of tumor embolus (p = 0.017) in HCC.
  
  
  CONCLUSIONS
  We first found hsa_circ_0001649 was significantly downregulated in HCC. Our findings indicate hsa_circ_0001649 might serve as a novel potential biomarker for HCC and may function in tumorigenesis and metastasis of HCC.
authors.affiliation: Shanghai Medical College of Fudan University, Shanghai, China.|State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Meilin
authors.initials: M
authors.lastname: Qin
authors.affiliation: Key Laboratory of Systems Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, China Academy of Scieneces, Shanghai, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Xisong
authors.initials: X
authors.lastname: Huo
authors.affiliation: State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Xuemei
authors.initials: X
authors.lastname: Tao
authors.affiliation: Shanghai Medical College of Fudan University, Shanghai, China.
authors.forename: Xiaomeng
authors.initials: X
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Zhouhong
authors.initials: Z
authors.lastname: Ge
authors.affiliation: Medical College of Nantong University, Nantong, Jiangsu, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Yang
authors.affiliation: Liver Cancer Institute, Zhongshan Hospital and Shanghai Medical College, Fudan University, Key Laboratory for Carcinogenesis and Cancer Invasion, the Chinese Ministry of Education, Shanghai, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Fan
authors.affiliation: Shanghai Medical College of Fudan University, Shanghai, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Wenxin
authors.initials: W
authors.lastname: Qin
mesh_terms: D000328:Adult; D000368:Aged; D015415:Biomarkers; D014408:Biomarkers, Tumor; D006528:Carcinoma, Hepatocellular / Q000175:diagnosis / Q000235:genetics*; D045744:Cell Line, Tumor; D004265:DNA Helicases / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008113:Liver Neoplasms / Q000175:diagnosis / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging; D012313:RNA / Q000737:chemistry / Q000235:genetics*; D000079962:RNA, Circular; D012372:ROC Curve; D017422:Sequence Analysis, DNA; D044767:Ubiquitin-Protein Ligases / Q000235:genetics
chemical_list: D015415:Biomarkers; D014408:Biomarkers, Tumor; D000079962:RNA, Circular; D012313:RNA; C516226:SHPRH protein, human; D044767:Ubiquitin-Protein Ligases; D004265:DNA Helicases
keywords: Circular RNA; biomarker; hepatocellular carcinoma; hsa_circ_0001649; hsa_circ_001599