title: Chip-Based Magnetic Solid-Phase Microextraction Online Coupled with MicroHPLC-ICPMS for the Determination of Mercury Species in Cells.
abstract: Trace mercury speciation in cells is critical to understand its cytotoxicity and cell protection mechanism. In this work, we fabricated a chip-based magnetic solid-phase microextraction (MSPME) system, integrating a cell lysis unit as well as a sample extraction unit, and online combined it with micro high-performance liquid chromatography (microHPLC)-inductively coupled plasma mass spectrometry (ICPMS) for the speciation of mercury in HepG2 cells. Magnetic nanoparticles with sulfhydryl functional group were synthesized and self-assembled in the microchannels for the preconcentration of mercury species in cells under an external magnetic field. The enrichment factors are ca. 10-fold, and the recoveries for the spiked samples are in the range of 98.3-106.5%. The developed method was used to analyze target mercury species in Hg(2+) or MeHg(+) incubated HepG2 cells. The results demonstrated that MeHg(+) entered into the HepG2 cells more easily than Hg(2+), and part of the MeHg(+) might demethylate into Hg(2+) in HepG2 cells. Besides, comprehensive speciation of mercury in incubated cells revealed different detoxification mechanisms of Hg(2+) and MeHg(+) in Hg(2+) or MeHg(+) incubated HepG2 cells.
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine, Ministry of Education, Department of Chemistry, Wuhan University , Wuhan 430072, China.
authors.forename: Han
authors.initials: H
authors.lastname: Wang
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine, Ministry of Education, Department of Chemistry, Wuhan University , Wuhan 430072, China.
authors.forename: Beibei
authors.initials: B
authors.lastname: Chen
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine, Ministry of Education, Department of Chemistry, Wuhan University , Wuhan 430072, China.
authors.forename: Siqi
authors.initials: S
authors.lastname: Zhu
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine, Ministry of Education, Department of Chemistry, Wuhan University , Wuhan 430072, China.
authors.forename: Xiaoxiao
authors.initials: X
authors.lastname: Yu
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine, Ministry of Education, Department of Chemistry, Wuhan University , Wuhan 430072, China.
authors.forename: Man
authors.initials: M
authors.lastname: He
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine, Ministry of Education, Department of Chemistry, Wuhan University , Wuhan 430072, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Hu
mesh_terms: D002851:Chromatography, High Pressure Liquid; D056945:Hep G2 Cells; D006801:Humans; D060526:Magnetic Fields; D013058:Mass Spectrometry; D008628:Mercury / Q000032:analysis*; D052617:Solid Phase Microextraction / Q000295:instrumentation
chemical_list: D008628:Mercury