title: Harpin Hpa1 Interacts with Aquaporin PIP1;4 to Promote the Substrate Transport and Photosynthesis in Arabidopsis.
abstract: Harpin proteins produced by plant-pathogenic Gram-negative bacteria are the venerable player in regulating bacterial virulence and inducing plant growth and defenses. A major gap in these effects is plant sensing linked to cellular responses, and plant sensor for harpin Hpa1 from rice bacterial blight pathogen points to plasma membrane intrinsic protein (PIP). Here we show that Arabidopsis AtPIP1;4 is a plasma membrane sensor of Hpa1 and plays a dual role in plasma membrane permeability of CO2 and H2O. In particular, AtPIP1;4 mediates CO2 transport with a substantial contribute to photosynthesis and further increases this function upon interacting with Hpa1 at the plasma membrane. As a result, leaf photosynthesis rates are increased and the plant growth is enhanced in contrast to the normal process without Hpa1-AtPIP1;4 interaction. Our findings demonstrate the first case that plant sensing of a bacterial harpin protein is connected with photosynthetic physiology to regulate plant growth.
authors.affiliation: Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
authors.affiliation: Research Group on Plant Biology under Mediterranean Conditions, Departament de Biologia, Universitat de les Illes Balears, Palma de Mallorca, Illes Balears 07122, Spain.
authors.forename: Jorge
authors.initials: J
authors.lastname: Gago
authors.affiliation: Institute of Grassland Science, Northeast Normal University and National Ministry of Education Key Laboratory of Vegetation Ecology, Changchun 130024, China.
authors.forename: Haiying
authors.initials: H
authors.lastname: Cui
authors.affiliation: South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China.
authors.forename: Zhengjiang
authors.initials: Z
authors.lastname: Qian
authors.affiliation: Agro-Meteorology Division, National Institute for Agro-Environmental Sciences, Tsukuba 305-8604, Japan.
authors.forename: Naomi
authors.initials: N
authors.lastname: Kodama
authors.affiliation: Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Hongtao
authors.initials: H
authors.lastname: Ji
authors.affiliation: Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Tian
authors.affiliation: Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Shen
authors.affiliation: Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Yanjuan
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Fengli
authors.initials: F
authors.lastname: Sun
authors.affiliation: Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Zhonglan
authors.initials: Z
authors.lastname: Xia
authors.affiliation: South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Ye
authors.affiliation: Institute of Grassland Science, Northeast Normal University and National Ministry of Education Key Laboratory of Vegetation Ecology, Changchun 130024, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Sun
authors.affiliation: Research Group on Plant Biology under Mediterranean Conditions, Departament de Biologia, Universitat de les Illes Balears, Palma de Mallorca, Illes Balears 07122, Spain.
authors.forename: Jaume
authors.initials: J
authors.lastname: Flexas
authors.affiliation: Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Hansong
authors.initials: H
authors.lastname: Dong
mesh_terms: D020346:Aquaporins / Q000378:metabolism*; D017360:Arabidopsis / Q000254:growth & development / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D001692:Biological Transport; D002245:Carbon Dioxide / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D005816:Genetic Complementation Test; D009154:Mutation / Q000235:genetics; D010788:Photosynthesis; D059828:Plant Cells / Q000378:metabolism; D011485:Protein Binding; D012441:Saccharomyces cerevisiae; D013329:Structure-Activity Relationship; D014867:Water / Q000378:metabolism
chemical_list: D020346:Aquaporins; D029681:Arabidopsis Proteins; D001426:Bacterial Proteins; C000611205:PIP1;4 protein, Arabidopsis; D014867:Water; D002245:Carbon Dioxide