title: Detection and analysis of tupaia hepatocytes via mAbs against tupaia serum albumin.
abstract: On the basis of its close phylogenetic relationship with primates, the development of Tupaia belangeri as an infection animal model and drug metabolism model could provide a new option for preclinical studies, especially in hepatitis virus research. As a replacement for primary human hepatocytes (PHHs), primary tupaia hepatocytes (PTHs) have been widely used. Similar to human serum albumin, tupaia serum albumin (TSA) is the most common liver synthesis protein and is an important biomarker for PTHs and liver function. However, no detection or quantitative method for TSA has been reported. In this study, mouse monoclonal antibodies (mAbs) 4G5 and 9H3 against TSA were developed to recognize PTHs, and they did not show cross-reactivity with serum albumin from common experimental animals, such as the mouse, rat, cow, rabbit, goat, monkey, and chicken. The two mAbs also exhibited good performance in fluorescence activated cell sorting (FACS) analysis and immunofluorescence (IF) detection of PTHs. A chemiluminescent enzyme immune assay method using the two mAbs, with a linear range from 96.89 pg/ml to 49,609.38 pg/ml, was developed for the quantitative detection of TSA. The mAbs and the CLEIA method provide useful tools for research on TSA and PTHs.
authors.affiliation: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in Infectious Diseases, Xiamen University, Xiamen, 361102, P.R. China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Liu
authors.forename: Lunzhi
authors.initials: L
authors.lastname: Yuan
authors.forename: Quan
authors.initials: Q
authors.lastname: Yuan
authors.forename: Yali
authors.initials: Y
authors.lastname: Zhang
authors.forename: Kun
authors.initials: K
authors.lastname: Wu
authors.forename: Tianying
authors.initials: T
authors.lastname: Zhang
authors.forename: Yong
authors.initials: Y
authors.lastname: Wu
authors.forename: Wangheng
authors.initials: W
authors.lastname: Hou
authors.forename: Tengyun
authors.initials: T
authors.lastname: Wang
authors.forename: Pingguo
authors.initials: P
authors.lastname: Liu
authors.forename: James Wai Kuo
authors.initials: JW
authors.lastname: Shih
authors.forename: Tong
authors.initials: T
authors.lastname: Cheng
authors.forename: Ningshao
authors.initials: N
authors.lastname: Xia
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D015415:Biomarkers / Q000097:blood; D004195:Disease Models, Animal; D005434:Flow Cytometry; D022781:Hepatocytes; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods*; D008163:Luminescent Measurements / Q000379:methods*; D051379:Mice; D023421:Models, Animal; D012709:Serum Albumin / Q000032:analysis* / Q000276:immunology*; D014417:Tupaia
chemical_list: D000911:Antibodies, Monoclonal; D015415:Biomarkers; D012709:Serum Albumin