title: Preparation of iron bound succinylated milk protein concentrate and evaluation of its stability.
abstract: Major problems associated with the fortification of soluble iron salts include chemical reactivity and incompatibility with other components. Milk protein concentrate (MPC) are able to bind significant amount of iron due to the presence of both casein and whey protein. MPC in its native state possess very poor solubility, therefore, succinylated derivatives of MPC (succ. MPC) were also used for the preparation of protein-iron complex. Preparation of the complex involved centrifugation (to remove insoluble iron), ultrafiltration (to remove unbound iron) and lyophilisation (to attain in dry form). Iron binding ability of MPC enhanced significantly (P<0.05) upon succinylation. Stability of bound iron from both varieties of complexes was monitored under different conditions encountered during processing. Higher stability (P<0.05) of bound iron was observed in succ. MPC-iron complex than native protein complex. This method could be adopted for the production of stable iron enriched protein, an organic iron source.
authors.affiliation: Dairy Chemistry Division, National Dairy Research Institute, Karnal, Haryana 132001, India. Electronic address: shilpashreebg14@gmail.com.
authors.forename: B G
authors.initials: BG
authors.lastname: Shilpashree
authors.affiliation: Dairy Chemistry Division, National Dairy Research Institute, Karnal, Haryana 132001, India. Electronic address: sumitak123@gmail.com.
authors.forename: Sumit
authors.initials: S
authors.lastname: Arora
authors.affiliation: Dairy Chemistry Division, National Dairy Research Institute, Karnal, Haryana 132001, India. Electronic address: vishk12000@yahoo.com.
authors.forename: Vivek
authors.initials: V
authors.lastname: Sharma
authors.affiliation: Dairy Chemistry Division, National Dairy Research Institute, Karnal, Haryana 132001, India. Electronic address: rbajaj1375@gmail.com.
authors.forename: Rajesh Kumar
authors.initials: RK
authors.lastname: Bajaj
authors.affiliation: Dairy Microbiology Division, National Dairy Research Institute, Karnal, Haryana 132001, India. Electronic address: sudhirndri@gmail.com.
authors.forename: S K
authors.initials: SK
authors.lastname: Tomar
mesh_terms: D000818:Animals; D002364:Caseins / Q000737:chemistry; D002417:Cattle; D006863:Hydrogen-Ion Concentration; D007501:Iron / Q000737:chemistry*; D008894:Milk Proteins / Q000737:chemistry*; D055550:Protein Stability; D014462:Ultrafiltration; D000067816:Whey Proteins / Q000737:chemistry
chemical_list: D002364:Caseins; D008894:Milk Proteins; D000067816:Whey Proteins; D007501:Iron
keywords: MPC–iron complex; Milk protein concentrate; Scanning electron microscope; Stability; Succinylation; Ultrafiltration