title: The distinct distribution and phylogenetic characteristics of dengue virus serotypes/genotypes during the 2013 outbreak in Yunnan, China: Phylogenetic characteristics of 2013 dengue outbreak in Yunnan, China.
abstract: Since 2000, sporadic imported cases of dengue fever were documented almost every year in Yunnan Province, China. Unexpectedly, a large-scale outbreak of dengue virus (DENV) infection occurred from August to December 2013, with 1538 documented cases. In the current study, 81 dengue-positive patient samples were collected from Xishuangbanna, the southernmost prefecture of the Yunnan province, and 23 from Dehong, the westernmost prefecture of the Yunnan province. The full-length envelope genes were amplified and sequenced. Phylogenetic analysis revealed that nine strains (39.1%) and 14 strains (60.9%) from the Dehong prefecture were classified as genotype I of DENV-1 and Asian I genotype of DENV-2, respectively. All strains from Xishuangbanna were identified as genotype II of DENV-3. Bayesian coalescent analysis indicates that the outbreak originated from bordering southeastern Asian countries. These three epidemic genotypes were predicted to originate in Thailand and then migrate into Yunnan through different routes.
authors.affiliation: Faculty of Life Science and Technology, Center for Molecular Medicine in Yunnan Province, Kunming University of Science and Technology, Yunnan, PR China. Electronic address: binghuiwang@aliyun.com.
authors.forename: Binghui
authors.initials: B
authors.lastname: Wang
authors.affiliation: Yunnan Research Institute of Parasitic Disease Control, Yunnan, PR China. Electronic address: yanghenglin@yipd.org.
authors.forename: Henglin
authors.initials: H
authors.lastname: Yang
authors.affiliation: Faculty of Life Science and Technology, Center for Molecular Medicine in Yunnan Province, Kunming University of Science and Technology, Yunnan, PR China. Electronic address: fyky2005@163.com.
authors.forename: Yue
authors.initials: Y
authors.lastname: Feng
authors.affiliation: Yunnan Research Institute of Parasitic Disease Control, Yunnan, PR China. Electronic address: zhouhn66@163.com.
authors.forename: Hongning
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Institute of Molecular Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Yunnan, PR China. Electronic address: djj@imbcams.com.cn.
authors.forename: Jiejie
authors.initials: J
authors.lastname: Dai
authors.affiliation: Institute of Molecular Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Yunnan, PR China. Electronic address: huyunzhang@imbcams.com.cn.
authors.forename: Yunzhang
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Faculty of Life Science and Technology, Center for Molecular Medicine in Yunnan Province, Kunming University of Science and Technology, Yunnan, PR China. Electronic address: 253091425@qq.com.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Faculty of Life Science and Technology, Center for Molecular Medicine in Yunnan Province, Kunming University of Science and Technology, Yunnan, PR China. Electronic address: yajuanwang1990@163.com.
authors.forename: Yajuan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Faculty of Life Science and Technology, Center for Molecular Medicine in Yunnan Province, Kunming University of Science and Technology, Yunnan, PR China. Electronic address: znbalooch@yahoo.com.
authors.forename: Zulqarnain
authors.initials: Z
authors.lastname: Baloch
authors.affiliation: Faculty of Life Science and Technology, Center for Molecular Medicine in Yunnan Province, Kunming University of Science and Technology, Yunnan, PR China. Electronic address: oliverxia2000@aliyun.com.
authors.forename: Xueshan
authors.initials: X
authors.lastname: Xia
mesh_terms: D001499:Bayes Theorem; D002681:China / Q000453:epidemiology; D003715:Dengue / Q000097:blood / Q000453:epidemiology* / Q000821:virology*; D003716:Dengue Virus / Q000145:classification* / Q000235:genetics*; D004196:Disease Outbreaks; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D010802:Phylogeny; D058974:Phylogeography; D065288:Serogroup; D014759:Viral Envelope Proteins / Q000235:genetics*
chemical_list: D014759:Viral Envelope Proteins
keywords: Bayesian analysis; Dengue; Imported infection; Outbreak; Phylogenetic analysis