title: The supramammillary nucleus and the claustrum activate the cortex during REM sleep.
abstract: Evidence in humans suggests that limbic cortices are more active during rapid eye movement (REM or paradoxical) sleep than during waking, a phenomenon fitting with the presence of vivid dreaming during this state. In that context, it seemed essential to determine which populations of cortical neurons are activated during REM sleep. Our aim in the present study is to fill this gap by combining gene expression analysis, functional neuroanatomy, and neurochemical lesions in rats. We find in rats that, during REM sleep hypersomnia compared to control and REM sleep deprivation, the dentate gyrus, claustrum, cortical amygdaloid nucleus, and medial entorhinal and retrosplenial cortices are the only cortical structures containing neurons with an increased expression of Bdnf, FOS, and ARC, known markers of activation and/or synaptic plasticity. Further, the dentate gyrus is the only cortical structure containing more FOS-labeled neurons during REM sleep hypersomnia than during waking. Combining FOS staining, retrograde labeling, and neurochemical lesion, we then provide evidence that FOS overexpression occurring in the cortex during REM sleep hypersomnia is due to projections from the supramammillary nucleus and the claustrum. Our results strongly suggest that only a subset of cortical and hippocampal neurons are activated and display plasticity during REM sleep by means of ascending projections from the claustrum and the supramammillary nucleus. Our results pave the way for future studies to identify the function of REM sleep with regard to dreaming and emotional memory processing.
authors.affiliation: UMR 5292 CNRS/U1028 INSERM, Centre de Recherche en Neurosciences de Lyon (CRNL), Team "Physiopathologie des réseaux neuronaux responsables du cycle veille-sommeil," Université Claude Bernard Lyon 1, Faculté de Médecine RTH Laennec, 7 Rue Guillaume Paradin, 69372 Lyon Cedex 08, France. ; College of Medical Sciences, Washington State University, 412 E. Spokane Falls Boulevard, PBS230, Spokane, WA 99202, USA.
authors.forename: Leslie
authors.initials: L
authors.lastname: Renouard
authors.affiliation: UMR 5292 CNRS/U1028 INSERM, Centre de Recherche en Neurosciences de Lyon (CRNL), Team "Physiopathologie des réseaux neuronaux responsables du cycle veille-sommeil," Université Claude Bernard Lyon 1, Faculté de Médecine RTH Laennec, 7 Rue Guillaume Paradin, 69372 Lyon Cedex 08, France.
authors.forename: Francesca
authors.initials: F
authors.lastname: Billwiller
authors.affiliation: UMR 5292 CNRS/U1028 INSERM, Centre de Recherche en Neurosciences de Lyon (CRNL), Team "Physiopathologie des réseaux neuronaux responsables du cycle veille-sommeil," Université Claude Bernard Lyon 1, Faculté de Médecine RTH Laennec, 7 Rue Guillaume Paradin, 69372 Lyon Cedex 08, France.
authors.forename: Keiko
authors.initials: K
authors.lastname: Ogawa
authors.affiliation: UMR 5292 CNRS/U1028 INSERM, Centre de Recherche en Neurosciences de Lyon (CRNL), Team "Physiopathologie des réseaux neuronaux responsables du cycle veille-sommeil," Université Claude Bernard Lyon 1, Faculté de Médecine RTH Laennec, 7 Rue Guillaume Paradin, 69372 Lyon Cedex 08, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Clément
authors.affiliation: UMR 5292 CNRS/U1028 INSERM, Centre de Recherche en Neurosciences de Lyon (CRNL), Team "Physiopathologie des réseaux neuronaux responsables du cycle veille-sommeil," Université Claude Bernard Lyon 1, Faculté de Médecine RTH Laennec, 7 Rue Guillaume Paradin, 69372 Lyon Cedex 08, France.
authors.forename: Nutabi
authors.initials: N
authors.lastname: Camargo
authors.affiliation: UMR 5292 CNRS/U1028 INSERM, Centre de Recherche en Neurosciences de Lyon (CRNL), Team "Physiopathologie des réseaux neuronaux responsables du cycle veille-sommeil," Université Claude Bernard Lyon 1, Faculté de Médecine RTH Laennec, 7 Rue Guillaume Paradin, 69372 Lyon Cedex 08, France.
authors.forename: Mouaadh
authors.initials: M
authors.lastname: Abdelkarim
authors.affiliation: UMR 5292 CNRS/U1028 INSERM, Centre de Recherche en Neurosciences de Lyon (CRNL), Team "Physiopathologie des réseaux neuronaux responsables du cycle veille-sommeil," Université Claude Bernard Lyon 1, Faculté de Médecine RTH Laennec, 7 Rue Guillaume Paradin, 69372 Lyon Cedex 08, France.
authors.forename: Nadine
authors.initials: N
authors.lastname: Gay
authors.affiliation: UMR 5292 CNRS/U1028 INSERM, Centre de Recherche en Neurosciences de Lyon (CRNL), Team "Physiopathologie des réseaux neuronaux responsables du cycle veille-sommeil," Université Claude Bernard Lyon 1, Faculté de Médecine RTH Laennec, 7 Rue Guillaume Paradin, 69372 Lyon Cedex 08, France.
authors.forename: Céline
authors.initials: C
authors.lastname: Scoté-Blachon
authors.affiliation: UMR 5292 CNRS/U1028 INSERM, Centre de Recherche en Neurosciences de Lyon (CRNL), Team "Physiopathologie des réseaux neuronaux responsables du cycle veille-sommeil," Université Claude Bernard Lyon 1, Faculté de Médecine RTH Laennec, 7 Rue Guillaume Paradin, 69372 Lyon Cedex 08, France.
authors.forename: Rouguy
authors.initials: R
authors.lastname: Touré
authors.affiliation: UMR 5292 CNRS/U1028 INSERM, Centre de Recherche en Neurosciences de Lyon (CRNL), Team "Physiopathologie des réseaux neuronaux responsables du cycle veille-sommeil," Université Claude Bernard Lyon 1, Faculté de Médecine RTH Laennec, 7 Rue Guillaume Paradin, 69372 Lyon Cedex 08, France.
authors.forename: Paul-Antoine
authors.initials: PA
authors.lastname: Libourel
authors.affiliation: UMR 5292 CNRS/U1028 INSERM, Centre de Recherche en Neurosciences de Lyon (CRNL), Team "Physiopathologie des réseaux neuronaux responsables du cycle veille-sommeil," Université Claude Bernard Lyon 1, Faculté de Médecine RTH Laennec, 7 Rue Guillaume Paradin, 69372 Lyon Cedex 08, France.
authors.forename: Pascal
authors.initials: P
authors.lastname: Ravassard
authors.affiliation: UMR 5292 CNRS/U1028 INSERM, Centre de Recherche en Neurosciences de Lyon (CRNL), Team "Physiopathologie des réseaux neuronaux responsables du cycle veille-sommeil," Université Claude Bernard Lyon 1, Faculté de Médecine RTH Laennec, 7 Rue Guillaume Paradin, 69372 Lyon Cedex 08, France.
authors.forename: Denise
authors.initials: D
authors.lastname: Salvert
authors.affiliation: UMR 5292 CNRS/U1028 INSERM, Centre de Recherche en Neurosciences de Lyon (CRNL), Team "Physiopathologie des réseaux neuronaux responsables du cycle veille-sommeil," Université Claude Bernard Lyon 1, Faculté de Médecine RTH Laennec, 7 Rue Guillaume Paradin, 69372 Lyon Cedex 08, France.
authors.forename: Christelle
authors.initials: C
authors.lastname: Peyron
authors.affiliation: Service de Radioanalyse, Centre de Médecine nucléaire, 59 Boulevard Pinel, 69677 Bron Cedex, France.
authors.forename: Bruno
authors.initials: B
authors.lastname: Claustrat
authors.affiliation: UMR 5292 CNRS/U1028 INSERM, Centre de Recherche en Neurosciences de Lyon (CRNL), Team "Physiopathologie des réseaux neuronaux responsables du cycle veille-sommeil," Université Claude Bernard Lyon 1, Faculté de Médecine RTH Laennec, 7 Rue Guillaume Paradin, 69372 Lyon Cedex 08, France.
authors.forename: Lucienne
authors.initials: L
authors.lastname: Léger
authors.affiliation: UMR 5292 CNRS/U1028 INSERM, Centre de Recherche en Neurosciences de Lyon (CRNL), Team "Physiopathologie des réseaux neuronaux responsables du cycle veille-sommeil," Université Claude Bernard Lyon 1, Faculté de Médecine RTH Laennec, 7 Rue Guillaume Paradin, 69372 Lyon Cedex 08, France.
authors.forename: Paul
authors.initials: P
authors.lastname: Salin
authors.affiliation: UMR 5292 CNRS/U1028 INSERM, Centre de Recherche en Neurosciences de Lyon (CRNL), Team "Physiopathologie des réseaux neuronaux responsables du cycle veille-sommeil," Université Claude Bernard Lyon 1, Faculté de Médecine RTH Laennec, 7 Rue Guillaume Paradin, 69372 Lyon Cedex 08, France.
authors.forename: Gael
authors.initials: G
authors.lastname: Malleret
authors.affiliation: UMR 5292 CNRS/U1028 INSERM, Centre de Recherche en Neurosciences de Lyon (CRNL), Team "Physiopathologie des réseaux neuronaux responsables du cycle veille-sommeil," Université Claude Bernard Lyon 1, Faculté de Médecine RTH Laennec, 7 Rue Guillaume Paradin, 69372 Lyon Cedex 08, France.
authors.forename: Patrice
authors.initials: P
authors.lastname: Fort
authors.affiliation: UMR 5292 CNRS/U1028 INSERM, Centre de Recherche en Neurosciences de Lyon (CRNL), Team "Physiopathologie des réseaux neuronaux responsables du cycle veille-sommeil," Université Claude Bernard Lyon 1, Faculté de Médecine RTH Laennec, 7 Rue Guillaume Paradin, 69372 Lyon Cedex 08, France.
authors.forename: Pierre-Hervé
authors.initials: PH
authors.lastname: Luppi
keywords: REM sleep; dentate gyrus; hippocampus; immediate early genes; supramammillary nucleus