title: AtCNGC2 is involved in jasmonic acid-induced calcium mobilization.
abstract: Calcium (Ca(2+)) mobilization is a central theme in various plant signal transduction pathways. We demonstrate that Arabidopsis thaliana cyclic nucleotide-gated channel 2 (AtCNGC2) is involved in jasmonic acid (JA)-induced apoplastic Ca(2+) influx in Arabidopsis epidermal cells. Ca(2+) imaging results showed that JA can induce an elevation in the cytosolic cAMP concentration ([cAMP]cyt), reaching a maximum within 3 min. Dibutyryl cAMP (db-cAMP), a cell membrane-permeable analogue of cAMP, induced an increase in the cytosolic Ca(2+) concentration ([Ca(2+)]cyt), with a peak at 4 min. This [Ca(2+)]cyt increase was triggered by the JA-induced increase in [cAMP]cyt. W-7[N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide], an antagonist of calmodulin, positively modulated the JA-induced increase in [Ca(2+)]cyt, while W-5[N-(6-aminohexyl)-1-naphthalenesulfonamide], an inactive antagonist of calmodulin, had no apparent effect. db-cAMP and JA positively induced the expression of primary (i.e. JAZ1 and MYC2) and secondary (i.e. VSP1) response genes in the JA signalling pathway in wild-type Arabidopsis thaliana, whereas they had no significant effect in the AtCNGC2 mutant 'defense, no death (dnd1) plants. These data provide evidence that JA first induces the elevation of cAMP, and cAMP, as an activating ligand, activates the AtCNGC2 channel, resulting in apoplastic Ca(2+) influx through AtCNGC2.
authors.affiliation: Plant Science and Technology College, Beijing University of Agriculture, Beijing 102206, China.
authors.forename: Min
authors.initials: M
authors.lastname: Lu
authors.affiliation: Plant Science and Technology College, Beijing University of Agriculture, Beijing 102206, China.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Plant Science and Technology College, Beijing University of Agriculture, Beijing 102206, China.
authors.forename: Shikun
authors.initials: S
authors.lastname: Tang
authors.affiliation: Plant Science and Technology College, Beijing University of Agriculture, Beijing 102206, China.
authors.forename: Jinbao
authors.initials: J
authors.lastname: Pan
authors.affiliation: College of Biological Science and Engineering, Beijing University of Agriculture, Beijing 102206, China.
authors.forename: Yongkun
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Plant Science and Technology College, Beijing University of Agriculture, Beijing 102206, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Han
authors.affiliation: Plant Science and Technology College, Beijing University of Agriculture, Beijing 102206, China School of Life Sciences, Shandong Normal University, Jinan 250014, China.
authors.forename: Yangyang
authors.initials: Y
authors.lastname: Li
authors.affiliation: School of Life Sciences, Shandong Normal University, Jinan 250014, China.
authors.forename: Xihua
authors.initials: X
authors.lastname: Du
authors.affiliation: Plant Science and Technology College, Beijing University of Agriculture, Beijing 102206, China.
authors.forename: Zhangjie
authors.initials: Z
authors.lastname: Nan
authors.affiliation: Plant Science and Technology College, Beijing University of Agriculture, Beijing 102206, China sunqp@bac.edu.cn.
authors.forename: Qingpeng
authors.initials: Q
authors.lastname: Sun
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D003994:Bucladesine / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D002147:Calmodulin / Q000037:antagonists & inhibitors / Q000378:metabolism; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D054815:Cyclic Nucleotide-Gated Cation Channels / Q000235:genetics / Q000378:metabolism*; D003517:Cyclopentanes / Q000494:pharmacology*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D008564:Membrane Potentials / Q000187:drug effects; D008954:Models, Biological; D054883:Oxylipins / Q000494:pharmacology*; D018515:Plant Leaves / Q000187:drug effects / Q000378:metabolism; D018517:Plant Roots / Q000033:anatomy & histology / Q000187:drug effects / Q000254:growth & development; D054046:Plant Stomata / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D013449:Sulfonamides / Q000494:pharmacology
chemical_list: D029681:Arabidopsis Proteins; C518228:CNGC2 protein, Arabidopsis; D002147:Calmodulin; D054815:Cyclic Nucleotide-Gated Cation Channels; D003517:Cyclopentanes; D054883:Oxylipins; D013449:Sulfonamides; D003994:Bucladesine; C017967:W 7; C011006:jasmonic acid; C034700:N-(6-aminohexyl)-1-naphthalenesulfonamide; D002118:Calcium
keywords: Arabidopsis thaliana; CNGC; Calcium influx; Cytosolic Ca2+; Jasmonic acid.; cAMP