title: Design of a Nanostructured Active Surface against Gram-Positive and Gram-Negative Bacteria through Plasma Activation and in Situ Silver Reduction.
abstract: Nowadays there is an increasing focus for avoiding bacterial colonization in a medical device after implantation. Bacterial infection associated with prosthesis implantation, or even along the lifetime of the implanted prosthesis, entails a serious problem, emphasized with immunocompromised patients. This work shows a new methodology to create highly hydrophobic micro-/nanostructured silver antibacterial surfaces against Gram-positive and Gram-negative bacteria, using low-pressure plasma. PDMS (polydimethylsiloxane) samples, typically used in tracheal prosthesis, are coated with PFM (pentafluorophenyl methacrylate) through PECVD (plasma enhance chemical vapor deposition) technique. PFM thin films offer highly reactive ester groups that allow them to react preferably with amine bearing molecules, such as amine sugar, to create controlled reductive surfaces capable of reducing silver salts to a nanostructured metallic silver. This micro-/nanostructured silver coating shows interesting antibacterial properties combined with an antifouling behavior causing a reduction of Gram-positive and Gram-negative bacteria viability. In addition, these types of silver-coated samples show no apparent cytotoxicity against COS-7 cells.
authors.affiliation: Grup d'Enginyeria de Materials (GEMAT), Institut Químic de Sarrià, Universitat Ramon Llull , Via Augusta 390, 08017 Barcelona, Spain.
authors.forename: Joan
authors.initials: J
authors.lastname: Gilabert-Porres
authors.forename: Sara
authors.initials: S
authors.lastname: Martí
authors.forename: Laura
authors.initials: L
authors.lastname: Calatayud
authors.affiliation: Grup d'Enginyeria de Materials (GEMAT), Institut Químic de Sarrià, Universitat Ramon Llull , Via Augusta 390, 08017 Barcelona, Spain.
authors.forename: Victor
authors.initials: V
authors.lastname: Ramos
authors.forename: Antoni
authors.initials: A
authors.lastname: Rosell
authors.affiliation: Grup d'Enginyeria de Materials (GEMAT), Institut Químic de Sarrià, Universitat Ramon Llull , Via Augusta 390, 08017 Barcelona, Spain.
authors.forename: Salvador
authors.initials: S
authors.lastname: Borrós
mesh_terms: D000818:Animals; D019556:COS Cells; D016923:Cell Death / Q000187:drug effects; D002522:Chlorocebus aethiops; D006090:Gram-Negative Bacteria / Q000187:drug effects*; D006094:Gram-Positive Bacteria / Q000187:drug effects*; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability / Q000187:drug effects; D049329:Nanostructures / Q000737:chemistry*; D010084:Oxidation-Reduction; D058626:Plasma Gases / Q000494:pharmacology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D012834:Silver / Q000494:pharmacology*; D018629:Spectrometry, Mass, Secondary Ion; D013211:Staphylococcus aureus / Q000187:drug effects; D013499:Surface Properties; D014867:Water / Q000737:chemistry
chemical_list: D058626:Plasma Gases; D014867:Water; D012834:Silver
keywords: PECVD; antibacterial; medical device; silver; surface engineering; trachea