title: Affinity Chromatography of Native and Recombinant Proteins from Receptors for Insulin and IGF-I to Recombinant Single Chain Antibodies.
abstract: Affinity chromatography is an efficient method to isolate proteins by taking advantage of their affinities for specific molecules such as substrates, inhibitors, antigens, ligands, antibodies, and other interacting molecules, including subunits. Nowadays, we take the effectiveness and excellence of this technology for granted. This essay will mainly cover the use of affinity chromatography based on my experience.
authors.affiliation: Department of Diabetes Complications and Metabolism, Beckman Research Institute of City of Hope , Duarte, CA , USA.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Fujita-Yamaguchi
keywords: IGF-I; affinity chromatography; insulin; receptors; single-chain antibodies