title: Chitosan-collagen porous scaffold and bone marrow mesenchymal stem cell transplantation for ischemic stroke.
abstract: In this study, we successfully constructed a composite of bone marrow mesenchymal stem cells and a chitosan-collagen scaffold in vitro, transplanted either the composite or bone marrow mesenchymal stem cells alone into the ischemic area in animal models, and compared their effects. At 14 days after co-transplantation of bone marrow mesenchymal stem cells and the hitosan-collagen scaffold, neurological function recovered noticeably. Vascular endothelial growth factor expression and nestin-labeled neural precursor cells were detected in the ischemic area, surrounding tissue, hippocampal dentate gyrus and subventricular zone. Simultaneously, a high level of expression of glial fibrillary acidic protein and a low level of expression of neuron-specific enolase were visible in BrdU-labeled bone marrow mesenchymal stem cells. These findings suggest that transplantation of a composite of bone marrow mesenchymal stem cells and a chitosan-collagen scaffold has a neuroprotective effect following ischemic stroke.
authors.affiliation: Department of Neurosurgery, the Third Affiliated Hospital of Xi'an Jiaotong University; Shaanxi Provincial People's Hospital, Xi'an, Shaanxi Province, China ; Department of Neurosurgery, the Fourth People's Hospital of Shaanxi, Xi'an, Shaanxi Province, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Yan
authors.affiliation: Department of Neurology, Tianjin Huanhu Hospital, Tianjin, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Yue
authors.affiliation: Department of Neurosurgery, the Third Affiliated Hospital of Xi'an Jiaotong University; Shaanxi Provincial People's Hospital, Xi'an, Shaanxi Province, China.
authors.forename: Yue-Lin
authors.identifier: 0000-0001-8000-5810
authors.initials: YL
authors.lastname: Zhang
authors.affiliation: Department of Neurosurgery, the Third Affiliated Hospital of Xi'an Jiaotong University; Shaanxi Provincial People's Hospital, Xi'an, Shaanxi Province, China.
authors.forename: Guo-Chao
authors.initials: GC
authors.lastname: Mao
authors.affiliation: Department of Neurosurgery, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi Province, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Gao
authors.affiliation: Department of Neurosurgery, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi Province, China.
authors.forename: Zhen-Xing
authors.initials: ZX
authors.lastname: Zuo
authors.affiliation: Department of Neurology, Tianjin Huanhu Hospital, Tianjin, China.
authors.forename: Ya-Jing
authors.initials: YJ
authors.lastname: Zhang
authors.affiliation: Department of Neurology, Tianjin Huanhu Hospital, Tianjin, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Lu
keywords: bone marrow mesenchymal stem cells; cell differentiation; cell transplantation; chitosan-collagen scaffold; ischemic stroke; nerve regeneration; neural regeneration; neurological function