title: Stereocontrol within polyketide assembly lines.
abstract: Most of the stereocenters of polyketide natural products are established during assembly line biosynthesis. The body of knowledge for how stereocenters are set is now large enough to begin constructing physical models of key reactions. Interactions between stereocenter-forming enzymes and polyketide intermediates are examined here at atomic resolution, drawing from the most current structural and functional information of ketosynthases (KSs), ketoreductases (KRs), dehydratases (DHs), enoylreductases (ERs), and related enzymes. While many details remain to be experimentally determined, our understanding of the chemical and physical mechanisms utilized by the chirality-molding enzymes of modular PKSs is rapidly advancing.
authors.affiliation: Department of Molecular Biosciences, The University of Texas at Austin, 2506 Speedway Stop A5000, Austin, TX 78712, USA. adriankc@utexas.edu and Department of Chemistry, The University of Texas at Austin, 105 E 24th St. Stop A5300, Austin, TX 78712, USA.
authors.forename: Adrian T
authors.initials: AT
authors.lastname: Keatinge-Clay
mesh_terms: D001688:Biological Products / Q000737:chemistry / Q000378:metabolism*; D015394:Molecular Structure; D061065:Polyketides / Q000737:chemistry / Q000378:metabolism*; D011487:Protein Conformation; D013237:Stereoisomerism
chemical_list: D001688:Biological Products; D061065:Polyketides