title: Transcriptome Profiling of Louisiana iris Root and Identification of Genes Involved in Lead-Stress Response.
abstract: Louisiana iris is tolerant to and accumulates the heavy metal lead (Pb). However, there is limited knowledge of the molecular mechanisms behind this feature. We describe the transcriptome of Louisiana iris using Illumina sequencing technology. The root transcriptome of Louisiana iris under control and Pb-stress conditions was sequenced. Overall, 525,498 transcripts representing 313,958 unigenes were assembled using the clean raw reads. Among them, 43,015 unigenes were annotated and their functions classified using the euKaryotic Orthologous Groups (KOG) database. They were divided into 25 molecular families. In the Gene Ontology (GO) database, 50,174 unigenes were categorized into three GO trees (molecular function, cellular component and biological process). After analysis of differentially expressed genes, some Pb-stress-related genes were selected, including biosynthesis genes of chelating compounds, metal transporters, transcription factors and antioxidant-related genes. This study not only lays a foundation for further studies on differential genes under Pb stress, but also facilitates the molecular breeding of Louisiana iris.
authors.affiliation: Institute of Botany, Jiangsu Province and Chinese Academy of Science, Nanjing 210014, China. 2012204030@njau.edu.cn.|Suzhou Polytechnical Institute of Agriculture, Suzhou 215008, China. 2012204030@njau.edu.cn.
authors.forename: Songqing
authors.initials: S
authors.lastname: Tian
authors.affiliation: Institute of Botany, Jiangsu Province and Chinese Academy of Science, Nanjing 210014, China. chunsungu@cnbg.net.
authors.forename: Chunsun
authors.initials: C
authors.lastname: Gu
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing 210014, China. 2013104113@njau.edu.cn.
authors.forename: Liangqin
authors.initials: L
authors.lastname: Liu
authors.affiliation: Institute of Botany, Jiangsu Province and Chinese Academy of Science, Nanjing 210014, China. 2010204026@njau.edu.cn.|Suzhou Polytechnical Institute of Agriculture, Suzhou 215008, China. 2010204026@njau.edu.cn.
authors.forename: Xudong
authors.initials: X
authors.lastname: Zhu
authors.affiliation: Institute of Botany, Jiangsu Province and Chinese Academy of Science, Nanjing 210014, China. 2014216028@njau.edu.cn.
authors.forename: Yanhai
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Institute of Botany, Jiangsu Province and Chinese Academy of Science, Nanjing 210014, China. hsz1959@cnbg.net.
authors.forename: Suzhen
authors.initials: S
authors.lastname: Huang
mesh_terms: D019295:Computational Biology / Q000379:methods; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D063990:Gene Ontology; D059014:High-Throughput Nucleotide Sequencing; D027621:Iridaceae / Q000187:drug effects* / Q000235:genetics*; D007854:Lead / Q000633:toxicity*; D058977:Molecular Sequence Annotation; D018517:Plant Roots / Q000235:genetics*; D015203:Reproducibility of Results; D013312:Stress, Physiological / Q000235:genetics*; D059467:Transcriptome
chemical_list: D007854:Lead
keywords: Iris; Lead; RT-qPCR; transcriptome