title: Curcumin Delivery by Poly(Lactide)-Based Co-Polymeric Micelles: An In Vitro Anticancer Study.
abstract: OBJECTIVE
  This work describes the synthesis of block co-polymeric micelles, methoxy-poly(ethylene glycol)-poly(D,L-lactide) (mPEG-PLA) to encapsulate Curcumin (CUR), thereby improving the dispersibility and chemical stability of curcumin, prolonging its cellular uptake and enhancing its bioavailability.
  
  
  METHODS
  CUR-mPEG-PLA micelles, was prepared using the thin-film hydration method and evaluated in vitro. The preparation process was optimized with a central composite design (CCD). Micelles were characterized by size, transmission electron microscopy, loading capacity, and critical micelle concentration (CMC). The cytotoxicity of CUR-mPEG-PLA micelles was investigated against murine melanoma cells, B16F10 and human breast cancer cells, MDA-MB-231.
  
  
  RESULTS
  The average size of the CUR-mPEG-PLA micelles was 110 ± 5 nm with polydispersity index in the range of 0.15-0.31, and the encapsulating efficiency for CUR was 91.89 ± 1.2, and 11.06 ± 0.8% for drug-loading. Sustained release of CUR from micelles was observed with 9.73% CUR release from micelles compared to 64.24% release of free curcumin in first 6 h under sink condition. The CUR-mPEG-PLA was efficiently taken up by the cancer cells, B16F10 and MDA-MB-231. Following 24 h incubation, CUR-mPEG-PLA induced higher cytotoxicity compared to free CUR in MDA-MB-231 cell lines indicating exposure of higher dose of free CUR to cells lead to up-regulation of drug efflux mechanisms leading to decreased cell death in case of free CUR administration.
  
  
  CONCLUSIONS
  Our results indicate that the proposed micellar system has the potential to serve as an efficient carrier for CUR by effectively solubilizing, stabilizing and delivering the drug in a controlled manner to the cancer cells.
authors.affiliation: Department of Pharmacy, BITS-Pilani, Hyderabad Campus, Shameerpet, Hyderabad, 500078, Telangana, India.
authors.forename: Preeti
authors.initials: P
authors.lastname: Kumari
authors.affiliation: Department of Pharmacy, BITS-Pilani, Hyderabad Campus, Shameerpet, Hyderabad, 500078, Telangana, India.
authors.forename: Muddineti Omkara
authors.initials: MO
authors.lastname: Swami
authors.affiliation: Department of Pharmacy, BITS-Pilani, Hyderabad Campus, Shameerpet, Hyderabad, 500078, Telangana, India.
authors.forename: Sravan Kumar
authors.initials: SK
authors.lastname: Nadipalli
authors.affiliation: Department of Pharmacy, BITS-Pilani, Hyderabad Campus, Shameerpet, Hyderabad, 500078, Telangana, India.
authors.forename: Srividya
authors.initials: S
authors.lastname: Myneni
authors.affiliation: Department of Pharmacy, BITS-Pilani, Hyderabad Campus, Shameerpet, Hyderabad, 500078, Telangana, India.
authors.forename: Balaram
authors.initials: B
authors.lastname: Ghosh
authors.affiliation: Department of Pharmacy, BITS-Pilani, Hyderabad Campus, Shameerpet, Hyderabad, 500078, Telangana, India. swati.biswas@hyderabad.bits-pilani.ac.in.
authors.forename: Swati
authors.initials: S
authors.lastname: Biswas
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000494:pharmacology*; D001940:Breast / Q000187:drug effects / Q000473:pathology; D001943:Breast Neoplasms / Q000188:drug therapy / Q000473:pathology; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D003474:Curcumin / Q000008:administration & dosage* / Q000494:pharmacology*; D004337:Drug Carriers / Q000737:chemistry*; D005260:Female; D006801:Humans; D008545:Melanoma / Q000188:drug therapy / Q000473:pathology; D051379:Mice; D008823:Micelles; D011091:Polyesters / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D004337:Drug Carriers; D008823:Micelles; D011091:Polyesters; C492425:methoxy poly(ethylene glycol)-poly(lactide); D011092:Polyethylene Glycols; D003474:Curcumin
keywords: cancer; curcumin; mPEG-PLA; polymeric micelles