title: Effects of dibutyryl cyclic adenosine monophosphate and human chorionic gonadotropin on the formation of antral follicle-like structures by bovine cumulus-oocyte complexes.
abstract: This study evaluated the effect of dibutyryl cyclic adenosine monophos-phate (dbcAMP) and human chorionic gonadotropin (hCG) on the formation of antral follicle-like structures (AFLSs) and on the meiotic status of bovine cumulus- oocyte complexes (COCs) embedded in collagen gel. Supplementation with dbcAMP increased the mean diameter of AFLSs during days 4-8 of culture compared with that of control COCs, irrespective of the concentration of dbcAMP used (0.5-2.0 mM). When the embedded COCs were cultured for 8 days with hCG, the diameters of AFLSs after 4 days of culture tended to be lower in the supplemented COCs than in the control COCs without hCG, irrespective of the concentration used (1-100 IU/mL). Supplementation with 10 IU/mL hCG increased the concentrations of anti-Müllerian hormone but not progesterone and oestradiol in the culture medium after 4 days of culture. Almost all oocytes collected from AFLSs had resumed meiosis by the end of culture, irrespective of supplementation of dbcAMP and hCG. These results indicate that although dbcAMP had a positive effect on AFLS formation and development, supplementation with hCG was detrimental. Moreover, hCG supplementation did not influence the luteinisation of granulosa cells in the AFLS for 4 days after the start of culture.
authors.affiliation: Laboratory of Animal Reproduction, The United Graduate School of Veterinary Science, Yamaguchi University , Yamaguchi 753-8515 , Japan.
authors.forename: Tetsushi
authors.initials: T
authors.lastname: Ono
authors.affiliation: Laboratory of Animal Reproduction, The United Graduate School of Veterinary Science, Yamaguchi University , Yamaguchi 753-8515 , Japan.
authors.forename: Asako
authors.initials: A
authors.lastname: Takaoka
authors.affiliation: Laboratory of Animal Reproduction, The United Graduate School of Veterinary Science, Yamaguchi University , Yamaguchi 753-8515 , Japan.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Morita
authors.affiliation: Laboratory of Animal Reproduction, The United Graduate School of Veterinary Science, Yamaguchi University , Yamaguchi 753-8515 , Japan.
authors.forename: Lanh
authors.initials: L
authors.lastname: Do
authors.affiliation: Laboratory of Animal Reproduction, The United Graduate School of Veterinary Science, Yamaguchi University , Yamaguchi 753-8515 , Japan.
authors.forename: Fuminori
authors.initials: F
authors.lastname: Tanihara
authors.affiliation: Laboratory of Animal Reproduction, The United Graduate School of Veterinary Science, Yamaguchi University , Yamaguchi 753-8515 , Japan.
authors.forename: Masayasu
authors.initials: M
authors.lastname: Taniguchi
authors.affiliation: Laboratory of Animal Reproduction, The United Graduate School of Veterinary Science, Yamaguchi University , Yamaguchi 753-8515 , Japan.
authors.forename: Takeshige
authors.initials: T
authors.lastname: Otoi
keywords: Antrum-like structures; cAMP; cumulus cells; follicular formation; gap junction