title: Effect of luteolin on gene expression in mouse H22 hepatoma cells.
abstract: The purpose of our study was to observe the effects of luteolin on the expression of the genes ICAM-1, LFA-3, and PCNA in H22 hepatoma tissue. Sixty ICR (Institute of Cancer Research) mice with H22 hepatoma were randomly divided into five groups: a normal saline control group, low-, medium-, and high-dose luteolin groups, and a cyclophosphamide group. The mice were euthanized the day after administration withdrawal and subcutaneous tumor tissue was extracted. Quantitative fluorescence RT-PCR was used to detect the expression of ICAM-1, LFA-3, and PCNA in H22 hepatoma tissue in the mice. Luteolin was found to up-regulate the expression of ICAM-1 in H22 hepatoma tissue, of which the middle-dose group had the most obvious effect, showing a significant difference (P < 0.01) as compared to the normal saline group. Each dose group of luteolin significantly down-regulated the expression of LFA-3 in H22 hepatoma tissue, showing significant differences as compared to the saline control group (P < 0.01). The medium- and high-dose luteolin groups significantly reduced the expression of PCNA in H22 hepatoma tissue of ICR mice, where the effect of the high-dose group was the most obvious, and the difference between the two luteolin groups and the normal saline group was statistically significant (P < 0.01). Luteolin may inhibit tumor angiogenesis and tumor cell proliferation by down-regulation of LFA- 3 and PCNA and up-regulation of ICAM-1 in tumor tissue of tumor-bearing mice, thereby achieving its anti-tumor effect.
authors.affiliation: Department of General Surgery, Affiliated Hospital of Inner Mongolia Medical University, Hohhot, China.
authors.forename: J X
authors.initials: JX
authors.lastname: Niu
authors.affiliation: Department of Physical Examination, Binzhou People's Hospital, Shandong, China.
authors.forename: H P
authors.initials: HP
authors.lastname: Guo
authors.affiliation: Intensive Care Unit, Binzhou People's Hospital, Shandong, China.
authors.forename: H M
authors.initials: HM
authors.lastname: Gan
authors.affiliation: Department of Pharmacy, Affiliated Hospital of Inner Mongolia Medical University, Hohhot, China.
authors.forename: L D
authors.initials: LD
authors.lastname: Bao
authors.affiliation: Department of General Surgery, Affiliated Hospital of Inner Mongolia Medical University, Hohhot, China renjianjun237@163.com.
authors.forename: J J
authors.initials: JJ
authors.lastname: Ren
mesh_terms: D000818:Animals; D018968:CD58 Antigens / Q000096:biosynthesis* / Q000235:genetics; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D005472:Fluorouracil / Q000008:administration & dosage; D015972:Gene Expression Regulation, Neoplastic; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis* / Q000235:genetics; D008113:Liver Neoplasms / Q000188:drug therapy / Q000235:genetics* / Q000473:pathology; D047311:Luteolin / Q000008:administration & dosage; D051379:Mice; D009389:Neovascularization, Pathologic / Q000188:drug therapy / Q000235:genetics* / Q000473:pathology; D018809:Proliferating Cell Nuclear Antigen / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D018968:CD58 Antigens; D018809:Proliferating Cell Nuclear Antigen; D018799:Intercellular Adhesion Molecule-1; D047311:Luteolin; D005472:Fluorouracil