title: RNA 1 and RNA 2 Genomic Segments of Chronic Bee Paralysis Virus Are Infectious and Induce Chronic Bee Paralysis Disease.
abstract: Chronic bee paralysis virus (CBPV) causes an infectious and contagious disease of adult honeybees. Its segmented genome is composed of two major positive single-stranded RNAs, RNA 1 (3,674 nt) and RNA 2 (2,305 nt). Three minor RNAs (about 1,000 nt each) have been described earlier but they were not detected by sequencing of CBPV genome. In this study, the results of in vivo inoculation of the two purified CBPV major RNAs are presented and demonstrate that RNA 1 and RNA 2 are infectious. Honeybees inoculated with 10(9) RNA copies per bee developed paralysis symptoms within 6 days after inoculation. The number of CBPV RNA copies increased significantly throughout the infection. Moreover, the negative strand of CBPV RNA was detected by RT-PCR, and CBPV particles were visualized by electronic microscopy in inoculated honeybees. Taken together, these results show that CBPV RNA 1 and CBPV RNA 2 segments can induce virus replication and produce CBPV virus particles. Therefore, the three minor RNAs described in early studies are not essential for virus replication. These data are crucial for the development of a reverse genetic system for CBPV.
authors.affiliation: Anses Sophia Antipolis, Unit of Bee Pathology, 105 route des Chappes, CS 20111, 06902 Sophia Antipolis, France ; Architecture et Fonction des Macromolécules Biologiques (AFMB), Aix Marseille University, CNRS, UMR 7257, Case 932, Campus de Luminy, 163 avenue de Luminy, 13288 Marseille Cedex 09, France.
authors.forename: Ibrahim
authors.initials: I
authors.lastname: Youssef
authors.affiliation: Anses Sophia Antipolis, Unit of Bee Pathology, 105 route des Chappes, CS 20111, 06902 Sophia Antipolis, France.
authors.forename: Frank
authors.initials: F
authors.lastname: Schurr
authors.affiliation: Architecture et Fonction des Macromolécules Biologiques (AFMB), Aix Marseille University, CNRS, UMR 7257, Case 932, Campus de Luminy, 163 avenue de Luminy, 13288 Marseille Cedex 09, France.
authors.forename: Adeline
authors.initials: A
authors.lastname: Goulet
authors.affiliation: Anses Sophia Antipolis, Unit of Bee Pathology, 105 route des Chappes, CS 20111, 06902 Sophia Antipolis, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Cougoule
authors.affiliation: Anses Sophia Antipolis, Unit of Bee Pathology, 105 route des Chappes, CS 20111, 06902 Sophia Antipolis, France.
authors.forename: Magali
authors.initials: M
authors.lastname: Ribière-Chabert
authors.affiliation: Architecture et Fonction des Macromolécules Biologiques (AFMB), Aix Marseille University, CNRS, UMR 7257, Case 932, Campus de Luminy, 163 avenue de Luminy, 13288 Marseille Cedex 09, France.
authors.forename: Hervé
authors.initials: H
authors.lastname: Darbon
authors.affiliation: Anses Sophia Antipolis, Unit of Bee Pathology, 105 route des Chappes, CS 20111, 06902 Sophia Antipolis, France.
authors.forename: Richard
authors.initials: R
authors.lastname: Thiéry
authors.affiliation: Anses Sophia Antipolis, Unit of Bee Pathology, 105 route des Chappes, CS 20111, 06902 Sophia Antipolis, France.
authors.forename: Eric
authors.initials: E
authors.lastname: Dubois
mesh_terms: D000818:Animals; D001516:Bees / Q000821:virology*; D016679:Genome, Viral; D007304:Insect Viruses / Q000235:genetics* / Q000472:pathogenicity* / Q000648:ultrastructure; D010802:Phylogeny; D012328:RNA Viruses / Q000235:genetics* / Q000472:pathogenicity*; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics*; D059386:Reverse Genetics; D014779:Virus Replication
chemical_list: D012367:RNA, Viral