title: [Investigational Study of Mesenchymal Stem Cells on Lung Cancer Cell Proliferation and Invasion].
abstract: OBJECTIVE
  Mesenchymal stem cells (MSC) are adult stem cells derived from mesoderm. Evidence has shown that MSC could migrate towards tumor tissue and differentiate into tumor associated fibroblast in tumor microenvironment, which influences tumor growth and metastasis. However, the reports of MSC in non-small cell lung cancer (NSCLC) are few and controversial. The aim of this study is to explore the chemotaxis of MSC towards NSCLC and to test the effects of MSC on the proliferation and invasion ability of NSCLC.
  
  
  METHODS
  Transwell assay was used to test MSC and NSCLC migration and invasion, and Thymidine incorporation assay was adopted to measure NSCLC cells proliferation. The expression of interleukin-6 (IL-6), insulinlike growth factor (IGF-1), vascular endothelial growth factor (VEGF) and dickkopf-related protein 1 (DKK1) of MSCs were determined by real time PCR. A549 lung cancer xenograft animal tumor model was set up to evaluate the MSC effect in vivo.
  
  
  RESULTS
  Lung cancer cells could attract MSC tropism. MSC conditioned medium favored lung cancer cell proliferation and lung cancer cells stimulated the expression of IL-6, IGF-1, VEGF and DKK1 on MSCs. In vivo animal study showed that the tumor with MSC injection grew much faster compared to control group.
  
  
  CONCLUSIONS
  MSCs could migrate towards NSCLC cells and favor tumor growth. In turn, NSCLC cells could stimulate the overexpression of cytokines on MSCs which are essential for the tumor growth.
authors.affiliation: Department of Lung Cancer Surgery, Tianjin Medical University General Hospital, Tianjin 300052, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Lung Cancer Surgery, Tianjin Medical University General Hospital, Tianjin 300052, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Lung Cancer Surgery, Tianjin Medical University General Hospital, Tianjin 300052, China.
authors.forename: Renwang
authors.initials: R
authors.lastname: Liu
authors.affiliation: Tianjin Key Laboratory of Lung Cancer Metastasis and Tumor Microenvironment, Tianjin Lung Cancer Institute, Tianjin Medical University General Hospital, Tianjin 300052, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Guo
authors.affiliation: Tianjin Medical University Cancer  Hospital, Tianjin 300060, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Xu
authors.affiliation: Department of Lung Cancer Surgery, Tianjin Medical University General Hospital, Tianjin 300052, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Lung Cancer Surgery, Tianjin Medical University General Hospital, Tianjin 300052, China.
authors.forename: Song
authors.initials: S
authors.lastname: Xu
mesh_terms: D000818:Animals; D002289:Carcinoma, Non-Small-Cell Lung / Q000276:immunology / Q000473:pathology*; D002465:Cell Movement; D049109:Cell Proliferation; D006801:Humans; D015850:Interleukin-6 / Q000032:analysis; D008175:Lung Neoplasms / Q000276:immunology / Q000473:pathology*; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D051379:Mice; D009361:Neoplasm Invasiveness; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis
chemical_list: D015850:Interleukin-6; D042461:Vascular Endothelial Growth Factor A