title: The Effects of Thyroid Hormones on Gene Expression of Acyl-Coenzyme A Thioesterases in Adipose Tissue and Liver of Mice.
abstract: BACKGROUND
  Thyroid hormones (TH) exert pleiotropic effects on glucose and lipid homeostasis. However, it is as yet unclear how TH regulate lipid storage and utilization in order to adapt to metabolic needs. Acyl-CoA thioesterases (ACOTs) have been proposed to play a regulatory role in the metabolism of fatty acids.
  
  
  OBJECTIVE
  We investigated the interaction between thyroid dysfunction and Acot expression in adipose tissues and livers of thyrotoxic and hypothyroid mice.
  
  
  METHODS
  Ten-week-old female C57BL/6NTac mice (n = 10/group) were made hyperthyroid by the application of L-thyroxine (2 µg/ml in drinking water) for 4 weeks. Hypothyroidism was induced in 10-week-old mice by feeding an iodine-free chow supplemented with 0.15% PTU for 4 weeks. We measured mRNA expression levels of Acot8, 11 and 13 in the liver and epididymal and inguinal white and brown adipose tissues (BAT). Furthermore, we investigated hepatic Acot gene expression in TRα- and TRβ-deficient mice.
  
  
  RESULTS
  We showed that the expression of Acot8, 11 and 13 is predominantly stimulated by a thyrotoxic state in the epididymal white adipose tissue. In contrast, hypothyroidism predominantly induces the expression of Acot8 in BAT in comparison with BAT of thyrotoxic and euthyroid mice (p < 0.01). However, no significant changes in Acot expression were observed in inguinal white adipose tissue. In liver, Acot gene expression is collectively elicited by a thyrotoxic state.
  
  
  CONCLUSIONS
  These data suggest that ACOTs are targets of TH and are likely to influence 3,5,3'-triiodo-L-thyronine-orchestrated mechanisms of lipid uptake, storage and utilization to adapt the regulation of metabolic demands.
authors.affiliation: Division of Endocrinology and Nephrology, Department of Medicine, Leipzig, Germany.
authors.forename: Kerstin
authors.initials: K
authors.lastname: Krause
authors.affiliation: Division of Endocrinology and Nephrology, Department of Medicine, Leipzig, Germany.
authors.forename: Juliane
authors.initials: J
authors.lastname: Weiner
authors.affiliation: Department of Endocrinology and Metabolism, University of Duisburg-Essen, Essen, Germany.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Hönes
authors.affiliation: Division of Endocrinology and Nephrology, Department of Medicine, Leipzig, Germany ; IFB Adiposity Diseases, Leipzig University Medical Centre, Leipzig, Germany.
authors.forename: Nora
authors.initials: N
authors.lastname: Klöting
authors.affiliation: Institute for Experimental Endocrinology, Charité-Universitätsmedizin Berlin, Berlin, Germany.
authors.forename: Eddy
authors.initials: E
authors.lastname: Rijntjes
authors.affiliation: Division of Endocrinology and Nephrology, Department of Medicine, Leipzig, Germany.
authors.forename: John T
authors.initials: JT
authors.lastname: Heiker
authors.affiliation: Division of Endocrinology and Nephrology, Department of Medicine, Leipzig, Germany.
authors.forename: Claudia
authors.initials: C
authors.lastname: Gebhardt
authors.affiliation: Institute for Experimental Endocrinology, Charité-Universitätsmedizin Berlin, Berlin, Germany.
authors.forename: Josef
authors.initials: J
authors.lastname: Köhrle
authors.affiliation: Department of Endocrinology and Metabolism, University of Duisburg-Essen, Essen, Germany.
authors.forename: Dagmar
authors.initials: D
authors.lastname: Führer
authors.affiliation: Department of Nuclear Medicine, University of Leipzig, Leipzig, Germany.
authors.forename: Karen
authors.initials: K
authors.lastname: Steinhoff
authors.affiliation: Department of Nuclear Medicine, University of Leipzig, Leipzig, Germany ; IFB Adiposity Diseases, Leipzig University Medical Centre, Leipzig, Germany.
authors.forename: Swen
authors.initials: S
authors.lastname: Hesse
authors.affiliation: Department of Endocrinology and Metabolism, University of Duisburg-Essen, Essen, Germany.
authors.forename: Lars C
authors.initials: LC
authors.lastname: Moeller
authors.affiliation: Division of Endocrinology and Nephrology, Department of Medicine, Leipzig, Germany.
authors.forename: Anke
authors.initials: A
authors.lastname: Tönjes
keywords: Acyl-CoA thioesterases; Brown adipose tissue; Fatty acids; Thyroid hormones