title: Highly selective enrichment of baicalin in rat plasma by boronic acid-functionalized core-shell magnetic microspheres: Validation and application to a pharmacokinetic study.
abstract: To the best of our knowledge, this study is the first to successfully apply a novel, highly selective enrichment technique based on boronic acid-functionalized core-shell magnetic microspheres (BA-Fe3O4@SiO2-Au@mSiO2) with a large surface area and uniform pore size, to determine the baicalin concentration in rat plasma by HPLC. By taking advantage of the special interaction between boronic acid and baicalin under alkaline conditions, as well as the microspheres' size exclusion ability, baicalin was selectively extracted from protein-rich biosamples, such as plasma, without any other pretreatment procedure except for a 10-min vortexing step. BA-Fe3O4@SiO2-Au@mSiO2 microsphere-adsorbed baicalin was straightforwardly and rapidly isolated from the matrix using a magnet. Baicalin was subsequently eluted from the microspheres under acidic conditions for 2min for further HPLC analysis. The extraction conditions, such as the amount of microspheres added, adsorption time, adsorption pH, and elution time and pH, were also determined. Furthermore, method validation, including the linear range, detection limit, precision, accuracy, and recovery, were determined. This newly developed method based on BA-Fe3O4@SiO2-Au@mSiO2 microspheres is a simple, accurate, selective, and green analytical preparatory technique for analyzing baicalin in rat plasma. This study will be further novel research on the analysis of complex plasma samples and the pharmacokinetics of drugs similar to baicalin.
authors.affiliation: Department of Pharmacy, Eye Ear Nose Throat Hospital of Fudan University, No. 83, Fenyang Road, Shanghai 200031, China. Electronic address: taominhuang@126.com.
authors.forename: Taomin
authors.initials: T
authors.lastname: Huang
authors.affiliation: Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, No. 220, Handan Road, Shanghai 200433, China.
authors.forename: Ya
authors.initials: Y
authors.lastname: Xiong
authors.affiliation: Department of Pharmacy, Eye Ear Nose Throat Hospital of Fudan University, No. 83, Fenyang Road, Shanghai 200031, China.
authors.forename: Nianzu
authors.initials: N
authors.lastname: Chen
authors.affiliation: Department of Pharmacy, Eye Ear Nose Throat Hospital of Fudan University, No. 83, Fenyang Road, Shanghai 200031, China.
authors.forename: Donglei
authors.initials: D
authors.lastname: Wang
authors.affiliation: Department of Pharmacy, Eye Ear Nose Throat Hospital of Fudan University, No. 83, Fenyang Road, Shanghai 200031, China.
authors.forename: Yonghua
authors.initials: Y
authors.lastname: Lai
authors.affiliation: Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, No. 220, Handan Road, Shanghai 200433, China. Electronic address: chdeng@fudan.edu.cn.
authors.forename: Chunhui
authors.initials: C
authors.lastname: Deng
mesh_terms: D000327:Adsorption; D000818:Animals; D001897:Boronic Acids / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D052203:Ferrosoferric Oxide / Q000737:chemistry*; D005419:Flavonoids / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D006046:Gold / Q000737:chemistry*; D055772:Green Chemistry Technology; D060328:Magnetic Phenomena; D008297:Male; D008863:Microspheres; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D001897:Boronic Acids; D005419:Flavonoids; C038044:baicalin; D006046:Gold; D012822:Silicon Dioxide; D052203:Ferrosoferric Oxide
keywords: Baicalin; Boronic acid; Core–shell magnetic microsphere; High-performance liquid chromatography; Pharmacokinetics