title: Effects of Aluminum Exposure on the Bone Stimulatory Growth Factors in Rats.
abstract: Aluminum (Al) is considered to be a potentially toxic metal and inhibits bone formation. Transforming growth factor β1 (TGF-β1) and bone morphogenetic protein 2 (BMP-2) play an important role in regulating the bone formation. Therefore, this study aimed to investigate the effects of Al on the TGF-β1 and BMP-2 in rats. In this study, Wistar rats were randomly divided into Al-treated group and control group. The Al-treated rats were provided with drinking water containing 100 mg/L AlCl3, and the control rats were given distilled water for 30, 60, and 90 days, respectively. Ten rats were sacrificed in each group every 30 days. The Al-treated rats showed lower body weight and higher serum and bone levels of Al compared with the control rats. The expression levels of TGF-β1 and BMP-2 were also significantly decreased in the Al-treated rats. Serum levels of bone gamma-carboxyglutamic acid protein (BGP), carboxy-terminal propeptide of type I procollagen (PICP), and bone alkaline phosphatase (B-ALP) were markedly lower in the Al-treated groups than in the control group. These results indicate that Al inhibits the expression of TGF-β1 and BMP-2 in bone, which inhibits the activity of osteoblasts and reduces the synthesis of BGP, B-ALP, and type I collagen, thereby inhibiting bone formation.
authors.affiliation: College of Animal Science, Jilin University, 5333 Xi'an Road, Changchun, Jilin, 130062, China.|College of Pharmaceutical Engineering, Jilin Agriculture Science and Technology College, Jilin City, 132000, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Li
authors.affiliation: College of Animal Science, Jilin University, 5333 Xi'an Road, Changchun, Jilin, 130062, China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Luo
authors.affiliation: College of Pharmaceutical Engineering, Jilin Agriculture Science and Technology College, Jilin City, 132000, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.affiliation: College of Animal Science, Jilin University, 5333 Xi'an Road, Changchun, Jilin, 130062, China.|College of Pharmaceutical Engineering, Jilin Agriculture Science and Technology College, Jilin City, 132000, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Zheng
authors.affiliation: College of Pharmaceutical Engineering, Jilin Agriculture Science and Technology College, Jilin City, 132000, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Liu
authors.affiliation: College of Animal Science, Jilin University, 5333 Xi'an Road, Changchun, Jilin, 130062, China. oyhs1966@126.com.
authors.forename: Hongsheng
authors.initials: H
authors.lastname: Ouyang
mesh_terms: D000077410:Aluminum Chloride; D017607:Aluminum Compounds / Q000008:administration & dosage / Q000032:analysis / Q000097:blood / Q000494:pharmacology*; D000818:Animals; D015153:Blotting, Western; D001835:Body Weight / Q000187:drug effects; D055396:Bone Morphogenetic Protein 2 / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D002712:Chlorides / Q000008:administration & dosage / Q000032:analysis / Q000097:blood / Q000494:pharmacology*; D020869:Gene Expression Profiling; D008297:Male; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D053773:Transforming Growth Factor beta1 / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*
chemical_list: D017607:Aluminum Compounds; D055396:Bone Morphogenetic Protein 2; D002712:Chlorides; D012333:RNA, Messenger; D053773:Transforming Growth Factor beta1; D000077410:Aluminum Chloride
keywords: Aluminum exposure; Bone formation; Bone stimulatory growth factors