title: Genetic Polymorphisms of Multidrug Resistance Gene-1 (MDR1/ABCB1) and Glutathione S-Transferase Gene and the Risk of Inflammatory Bowel Disease among Moroccan Patients.
abstract: Inflammatory bowel diseases (IBD) are multifactorial disorders resulting from environmental and genetic factors. Polymorphisms in MDR1 and GSTs genes might explain individual differences in susceptibility to IBD. We carried out a case-control study to examine the association of MDR1 (C1236T and C3435T), GSTT1, and GSTM1 polymorphisms with the risk of IBD. Subjects were genotyped using PCR-RFLP for MDR1 gene and multiplex PCR for GSTT1 and GSTM1. Meta-analysis was performed to test the association of variant allele carriage with IBD risk. We report that GSTT1 null genotype is significantly associated with the risk of CD (OR: 2.5, CI: 1.2-5, P = 0.013) and UC (OR: 3.5, CI: 1.5-8.5, P = 0.004) and can influence Crohn's disease behavior. The interaction between GSTT1 and GSTM1 genes showed that the combined null genotypes were associated with the risk of UC (OR: 3.1, CI: 1.1-9, P = 0.049). Furthermore, when compared to combined 1236CC/CT genotypes, the 1236TT genotype of MDR1 gene was associated with the risk of UC (OR: 3.7, CI: 1.3-10.7, P = 0.03). Meta-analysis demonstrated significantly higher frequencies of 3435T carriage in IBD patients. Our results show that GSTT1 null and MDR1 polymorphisms could play a role in susceptibility to IBD.
authors.affiliation: Laboratory of Genetics and Molecular Pathologies, Faculty of Medicine and Pharmacy of Casablanca, Casablanca, Morocco.
authors.forename: Nezha
authors.initials: N
authors.lastname: Senhaji
authors.affiliation: Laboratory of Genetics and Molecular Pathologies, Faculty of Medicine and Pharmacy of Casablanca, Casablanca, Morocco.
authors.forename: Yaya
authors.initials: Y
authors.lastname: Kassogue
authors.affiliation: Gastroenterology Department, CHU Ibn Rochd, Casablanca, Morocco.
authors.forename: Mina
authors.initials: M
authors.lastname: Fahimi
authors.affiliation: Laboratory of Genetics and Molecular Pathologies, Faculty of Medicine and Pharmacy of Casablanca, Casablanca, Morocco.
authors.forename: Nadia
authors.initials: N
authors.lastname: Serbati
authors.affiliation: Gastroenterology Department, CHU Ibn Rochd, Casablanca, Morocco.
authors.forename: Wafaa
authors.initials: W
authors.lastname: Badre
authors.affiliation: Laboratory of Genetics and Molecular Pathologies, Faculty of Medicine and Pharmacy of Casablanca, Casablanca, Morocco.
authors.forename: Sellama
authors.initials: S
authors.lastname: Nadifi
mesh_terms: D018435:ATP Binding Cassette Transporter, Subfamily B / Q000235:genetics; D016022:Case-Control Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D015212:Inflammatory Bowel Diseases / Q000209:etiology / Q000235:genetics*; D011110:Polymorphism, Genetic; D012306:Risk
chemical_list: D018435:ATP Binding Cassette Transporter, Subfamily B; D005982:Glutathione Transferase; C513055:ABCB1 protein, human; C413545:glutathione S-transferase T1