title: Comparison of two enzymatic immunoassays, high resolution mass spectrometry method and radioimmunoassay for the quantification of human plasma histamine.
abstract: Histamine (HA) is one of the main immediate mediators involved in allergic reactions. HA plasma concentration is well correlated with the severity of vascular and respiratory signs of anaphylaxis. Consequently, plasma quantification of HA is useful to comfort the diagnosis of anaphylaxis. Currently, radioimmunoassay (RIA) is the gold standard method to quantify HA due to its high sensitivity, but it is time consuming, implicates specific formations and cautions for technicians, and produces hazardous radioactive wastes. The aim of this study was to compare two enzymatic immunoassays (EIA) and one in-house liquid chromatography high-resolution mass spectrometry method (LC-HRMS) with the gold standard method for HA quantification in plasma samples of patients suspected of anaphylaxis reactions. Ninety-two plasma samples were tested with the 4 methods (RIA, 2 EIA and LC-HRMS) for HA quantification. Fifty-eight samples displayed HA concentrations above the positive cut-off of 10nM evaluated by RIA, including 18 highly positive samples (>100 nM). This study shows that Immunotech(®) EIA and LC-HRMS concentrations were highly correlated with RIA values, in particular for samples with a HA concentration around the positive cut-off. In our hands, plasma concentrations obtained with the Demeditec Diagnostics(®) EIA correlated less with results obtained by RIA, and an underestimation of plasma HA levels led to a lack of sensitivity. In conclusion, this study demonstrates that Immunotech(®) EIA and LC-HRMS method could be used instead of RIA to assess plasma HA in human diagnostic use.
authors.affiliation: Université d'Angers, Faculté de Médecine, 49100 Angers, France; INSERM, Unit 892, 49100 Angers, France; CNRS, Unit 6299, Angers, France; LabEx IGO "ImmunoGraft Oncology", France; CHU Angers, Laboratoire d'Immunologie et d'Allergologie, Pôle Biologie, 49100 Angers, France.
authors.forename: Caroline
authors.initials: C
authors.lastname: Poli
authors.affiliation: CHU Rennes, Laboratoire d'Immunologie, Thérapie Cellulaire et Hématopoïèse, Pôle Biologie, 35033 Rennes, France; CHU Rennes, Laboratoire de Biochimie, Pôle Biologie, 35033 Rennes, France.
authors.forename: Mathieu
authors.initials: M
authors.lastname: Laurichesse
authors.affiliation: CHU Rennes, Laboratoire d'Immunologie, Thérapie Cellulaire et Hématopoïèse, Pôle Biologie, 35033 Rennes, France.
authors.forename: Octavie
authors.initials: O
authors.lastname: Rostan
authors.affiliation: CHU Rennes, Laboratoire d'Hématologie, Pôle Biologie, 35033 Rennes, France.
authors.forename: Delphine
authors.initials: D
authors.lastname: Rossille
authors.affiliation: Université d'Angers, Faculté de Médecine, 49100 Angers, France; INSERM, Unit 892, 49100 Angers, France; CNRS, Unit 6299, Angers, France; LabEx IGO "ImmunoGraft Oncology", France; CHU Angers, Laboratoire d'Immunologie et d'Allergologie, Pôle Biologie, 49100 Angers, France.
authors.forename: Pascale
authors.initials: P
authors.lastname: Jeannin
authors.affiliation: CHU Angers, Unité d'Allergologie générale, Département de Pneumologie, 49100 Angers, France.
authors.forename: Martine
authors.initials: M
authors.lastname: Drouet
authors.affiliation: CHU Angers, Laboratoire d'Immunologie et d'Allergologie, Pôle Biologie, 49100 Angers, France.
authors.forename: Gilles
authors.initials: G
authors.lastname: Renier
authors.affiliation: Université d'Angers, Faculté de Médecine, 49100 Angers, France; INSERM, Unit 892, 49100 Angers, France; CNRS, Unit 6299, Angers, France; LabEx IGO "ImmunoGraft Oncology", France; CHU Angers, Laboratoire d'Immunologie et d'Allergologie, Pôle Biologie, 49100 Angers, France.
authors.forename: Alain
authors.initials: A
authors.lastname: Chevailler
authors.affiliation: LabEx IGO "ImmunoGraft Oncology", France; CHU Rennes, Laboratoire d'Immunologie, Thérapie Cellulaire et Hématopoïèse, Pôle Biologie, 35033 Rennes, France; Université de Rennes 1, Faculté de Médecine, 35043 Rennes, France; INSERM Unité Mixte de Recherche U917, 35043 Rennes, France.
authors.forename: Karin
authors.initials: K
authors.lastname: Tarte
authors.affiliation: CHU Rennes, Laboratoire de Biochimie, Pôle Biologie, 35033 Rennes, France; Université de Rennes 1, Faculté de Médecine, 35043 Rennes, France; INSERM, Unité Mixte de Recherche U991, 35043 Rennes, France.
authors.forename: Claude
authors.initials: C
authors.lastname: Bendavid
authors.affiliation: Université d'Angers, Faculté de Médecine, 49100 Angers, France; INSERM, Unit 892, 49100 Angers, France; CNRS, Unit 6299, Angers, France; LabEx IGO "ImmunoGraft Oncology", France; CHU Angers, Laboratoire d'Immunologie et d'Allergologie, Pôle Biologie, 49100 Angers, France. Electronic address: celine.beauvillain@chu-angers.fr.
authors.forename: Céline
authors.initials: C
authors.lastname: Beauvillain
authors.affiliation: LabEx IGO "ImmunoGraft Oncology", France; CHU Rennes, Laboratoire d'Immunologie, Thérapie Cellulaire et Hématopoïèse, Pôle Biologie, 35033 Rennes, France; Université de Rennes 1, Faculté de Médecine, 35043 Rennes, France; INSERM Unité Mixte de Recherche U917, 35043 Rennes, France. Electronic address: patricia.ame-thomas@chu-rennes.fr.
authors.forename: Patricia
authors.initials: P
authors.lastname: Amé-Thomas
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods / Q000592:standards; D006632:Histamine / Q000097:blood*; D006801:Humans; D007124:Immunoenzyme Techniques; D013058:Mass Spectrometry / Q000379:methods / Q000592:standards; D011863:Radioimmunoassay / Q000379:methods / Q000592:standards; D053719:Tandem Mass Spectrometry / Q000379:methods* / Q000592:standards*
chemical_list: D006632:Histamine
keywords: Enzymatic immunoassay; Histamine; Mass spectrometry; Radioimmunoassay