title: Anti-obesity and anti-diabetic effects of flavonoid derivative (Fla-CN) via microRNA in high fat diet induced obesity mice.
abstract: 3-O-[(E)-4-(4-cyanophenyl)-2-oxobut-3-en-1-yl]kaempferol (Fla-CN), a semi-synthesized flavonoid derivative of tiliroside, reduces whole-body adiposity, ameliorates metabolic lipid disorder, improves insulin sensitivity and benefits other disorders characterized by insulin resistance in high fat diet induced obesity mice. The improvement of insulin sensitivity and the reduction of weight gain are correlated with the changes of leptin and adiponectin levels. As a result, Fla-CN treatment could increase the expressions of pAMPK and miR-27 in the liver and adipose tissues. Meanwhile, we discovered that the expressions of various adipogenesis genes were downregulated, which were target genes of miR-27. This is the first report for the action of miR-27 by flavonoid derivative in rodents. The action of Fla-CN might be through multiple approaches including AMPK activation and enhancement in miR-27 expression.
authors.affiliation: Research Center of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, People's Republic of China; Tianjin Key Laboratory on Technologies Enabling Development Clinical Therapeutics and Diagnostics (Theranostics), School of Pharmacy, Tianjin Medical University, Tianjin 300070, People's Republic of China.
authors.forename: Nan
authors.initials: N
authors.lastname: Qin
authors.affiliation: Research Center of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, People's Republic of China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Tianjin Key Laboratory on Technologies Enabling Development Clinical Therapeutics and Diagnostics (Theranostics), School of Pharmacy, Tianjin Medical University, Tianjin 300070, People's Republic of China.
authors.forename: Mei-Na
authors.initials: MN
authors.lastname: Jin
authors.affiliation: Tianjin Key Laboratory on Technologies Enabling Development Clinical Therapeutics and Diagnostics (Theranostics), School of Pharmacy, Tianjin Medical University, Tianjin 300070, People's Republic of China.
authors.forename: Chang
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Tianjin Key Laboratory on Technologies Enabling Development Clinical Therapeutics and Diagnostics (Theranostics), School of Pharmacy, Tianjin Medical University, Tianjin 300070, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Qiao
authors.affiliation: Tianjin Key Laboratory on Technologies Enabling Development Clinical Therapeutics and Diagnostics (Theranostics), School of Pharmacy, Tianjin Medical University, Tianjin 300070, People's Republic of China.
authors.forename: Xiao-Long
authors.initials: XL
authors.lastname: Yue
authors.affiliation: Research Center of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, People's Republic of China; Tianjin Key Laboratory on Technologies Enabling Development Clinical Therapeutics and Diagnostics (Theranostics), School of Pharmacy, Tianjin Medical University, Tianjin 300070, People's Republic of China. Electronic address: duanhq@tmu.edu.cn.
authors.forename: Hong-Quan
authors.initials: HQ
authors.lastname: Duan
authors.affiliation: Department of Immunology, Key Laboratory of Immune Microenvironment and Disease of the Educational Ministry of China, People's Republic of China. Electronic address: wniu@tmu.edu.cn.
authors.forename: Wen-Yan
authors.initials: WY
authors.lastname: Niu
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000378:metabolism; D000103:Acetyl-CoA Carboxylase / Q000378:metabolism; D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D000818:Animals; D019440:Anti-Obesity Agents / Q000494:pharmacology / Q000627:therapeutic use*; D003920:Diabetes Mellitus / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D059305:Diet, High-Fat; D005419:Flavonoids; D007004:Hypoglycemic Agents / Q000494:pharmacology / Q000627:therapeutic use*; D007333:Insulin Resistance; D044949:Kaempferols / Q000494:pharmacology / Q000627:therapeutic use*; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008297:Male; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000235:genetics; D018482:Muscle, Skeletal / Q000187:drug effects / Q000378:metabolism; D009765:Obesity / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology
chemical_list: C000611534:3-O-(4-(4-cyanophenyl)-2-oxobut-3-en-1-yl)kaempferol; D019440:Anti-Obesity Agents; D005419:Flavonoids; D007004:Hypoglycemic Agents; D044949:Kaempferols; D035683:MicroRNAs; C540674:Mirn27 microRNA, mouse; D055372:AMP-Activated Protein Kinases; D000103:Acetyl-CoA Carboxylase
keywords: AMPK; Anti-diabetes; Anti-obesity; Diet induced obesity; Flavonoid derivative; miR-27