title: S1PR1 expression correlates with inflammatory responses to Newcastle disease virus infection.
abstract: Newcastle disease virus (NDV) is the causative agent of Newcastle disease, which is characterized by inflammatory pathological changes in the organs of chickens. The inflammatory response to this disease has not been well characterized. Previous reports showed that the sphingosine-1-phosphate-1 receptor (S1PR1), a G protein-coupled receptor, is important to the activation of inflammatory responses. To understand better the viral pathogenesis and host inflammatory response, we analyzed S1PR1 expression during NDV infection. We observed a direct correlation between chicken embryo fibroblast (CEF) cellular inflammatory responses and S1PR1 expression. Virulent NDV-infected CEF cells also had elevated levels of pro-inflammatory cytokines (IL-1β, IL-6 and IL-18). When S1PR1 was inhibited by using the specific antagonist W146, pro-inflammatory cytokine production declined. Overexpression of S1PR1 resulted in increased virus-induced IL-1β production. S1PR1 expression levels did not impact significantly NDV replication. These findings highlight the important role of S1PR1 in inflammatory responses in NDV infection.
authors.affiliation: National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control, China; Key Laboratory of Animal Vaccine Development, Ministry of Agriculture, China; College of Veterinary Medicine, South China Agricultural University, 483 Wushan Road, Guangzhou 510642, China.
authors.forename: Yaling
authors.initials: Y
authors.lastname: Li
authors.affiliation: National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control, China; Key Laboratory of Animal Vaccine Development, Ministry of Agriculture, China; College of Veterinary Medicine, South China Agricultural University, 483 Wushan Road, Guangzhou 510642, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Xie
authors.affiliation: National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control, China; Key Laboratory of Animal Vaccine Development, Ministry of Agriculture, China; College of Veterinary Medicine, South China Agricultural University, 483 Wushan Road, Guangzhou 510642, China.
authors.forename: Minhua
authors.initials: M
authors.lastname: Sun
authors.affiliation: National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control, China; Key Laboratory of Animal Vaccine Development, Ministry of Agriculture, China; College of Veterinary Medicine, South China Agricultural University, 483 Wushan Road, Guangzhou 510642, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Xiang
authors.affiliation: National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control, China; Key Laboratory of Animal Vaccine Development, Ministry of Agriculture, China; College of Veterinary Medicine, South China Agricultural University, 483 Wushan Road, Guangzhou 510642, China.
authors.forename: Yinfeng
authors.initials: Y
authors.lastname: Kang
authors.affiliation: National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control, China; Key Laboratory of Animal Vaccine Development, Ministry of Agriculture, China; College of Veterinary Medicine, South China Agricultural University, 483 Wushan Road, Guangzhou 510642, China.
authors.forename: Pei
authors.initials: P
authors.lastname: Gao
authors.affiliation: National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control, China; Key Laboratory of Animal Vaccine Development, Ministry of Agriculture, China; College of Veterinary Medicine, South China Agricultural University, 483 Wushan Road, Guangzhou 510642, China.
authors.forename: Wenxian
authors.initials: W
authors.lastname: Zhu
authors.affiliation: National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control, China; Key Laboratory of Animal Vaccine Development, Ministry of Agriculture, China; College of Veterinary Medicine, South China Agricultural University, 483 Wushan Road, Guangzhou 510642, China. Electronic address: ningzhyong@scau.edu.cn.
authors.forename: Zhangyong
authors.initials: Z
authors.lastname: Ning
authors.affiliation: National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control, China; Key Laboratory of Animal Vaccine Development, Ministry of Agriculture, China; College of Veterinary Medicine, South China Agricultural University, 483 Wushan Road, Guangzhou 510642, China. Electronic address: rentao6868@126.com.
authors.forename: Tao
authors.initials: T
authors.lastname: Ren
mesh_terms: D000813:Anilides / Q000494:pharmacology; D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D016207:Cytokines / Q000235:genetics* / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000276:immunology / Q000821:virology; D009521:Newcastle Disease / Q000235:genetics / Q000276:immunology* / Q000821:virology; D009522:Newcastle disease virus / Q000472:pathogenicity; D063065:Organophosphonates / Q000494:pharmacology; D011201:Poultry Diseases / Q000235:genetics / Q000276:immunology / Q000821:virology*; D049349:Receptors, Lysosphingolipid / Q000235:genetics* / Q000378:metabolism; D015854:Up-Regulation
chemical_list: C513113:3-amino-4-(3-hexylphenylamino)-4-oxobutylphosphonic acid; D000813:Anilides; D016207:Cytokines; D063065:Organophosphonates; D049349:Receptors, Lysosphingolipid
keywords: Cytokines; Inflammatory response; Newcastle disease virus (NDV); S1PR1