title: High Frequency of Diarrheagenic Escherichia coli in HIV-Infected Patients and Patients with Thalassemia in Kerman, Iran.
abstract: This study was conducted on patients with thalassemia and HIV-infected patients to determine the frequency of diarrheagenic Escherichia coli in Kerman, Iran. We analyzed 68 and 49 E coli isolates isolated from healthy fecal samples of patients with thalassemia and HIV-infected patients, respectively. The E coli isolates were studied using a multiplex polymerase chain reaction to identify the enterotoxigenic E coli (ETEC), enterohemorrhagic E coli (EHEC), and enteropathogenic E coli (EPEC) groups. Statistical analysis was carried out to determine the correlation of diarrheagenic E coli between HIV-infected patients and patients with thalassemia using Stata 11.2 software. The frequency of having at least 1 diarrheagenic E coli was more common in patients with thalassemia (67.64%) than in HIV-infected patients (57.14%; P = .25), including ETEC (67.64% versus 57.14%), EHEC (33.82% versus 26.53%), and EPEC (19.11% versus 16.32%). The results of this study indicate that ETEC, EHEC, and EPEC pathotypes are widespread among diarrheagenic E coli isolates in patients with thalassemia and HIV-infected patients.
authors.affiliation: 1 Department of Microbiology, Sirjan Faculty of Medical Sciences, Kerman University of Medical Sciences, Kerman, Iran.|2 Regional Knowledge Hub and WHO Collaborating Centre for HIV Surveillance, Institute for Futures Studies in Health, Kerman University of Medical Sciences, Kerman, Iran.
authors.forename: Hesam
authors.initials: H
authors.lastname: Alizade
authors.affiliation: 2 Regional Knowledge Hub and WHO Collaborating Centre for HIV Surveillance, Institute for Futures Studies in Health, Kerman University of Medical Sciences, Kerman, Iran.|3 Department of Food Hygiene and Public Health, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran.
authors.forename: Hamid
authors.initials: H
authors.lastname: Sharifi
authors.affiliation: 4 Department of Molecular Microbiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran.
authors.forename: Zahedeh
authors.initials: Z
authors.lastname: Naderi
authors.affiliation: 4 Department of Molecular Microbiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Ghanbarpour
authors.affiliation: 5 Leishmaniasis Research Center, Kerman University of Medical Sciences, Kerman, Iran.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Bamorovat
authors.affiliation: 6 Research Center for Tropical and Infectious Diseases, Kerman University of Medical Sciences, Kerman, Iran.
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Aflatoonian
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics; D001427:Bacterial Toxins / Q000235:genetics; D060085:Coinfection / Q000453:epidemiology* / Q000382:microbiology; D003430:Cross-Sectional Studies; D003967:Diarrhea / Q000382:microbiology; D054324:Enterohemorrhagic Escherichia coli / Q000235:genetics / Q000302:isolation & purification; D054308:Enteropathogenic Escherichia coli / Q000235:genetics / Q000302:isolation & purification; D054307:Enterotoxigenic Escherichia coli / Q000235:genetics / Q000302:isolation & purification; D004768:Enterotoxins / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000150:complications / Q000453:epidemiology* / Q000382:microbiology*; D029968:Escherichia coli Proteins / Q000235:genetics; D005243:Feces / Q000382:microbiology; D005260:Female; D015658:HIV Infections / Q000453:epidemiology*; D006801:Humans; D007492:Iran / Q000453:epidemiology; D008297:Male; D015995:Prevalence; D022622:Shiga Toxin 1 / Q000235:genetics; D022641:Shiga Toxin 2 / Q000235:genetics; D013789:Thalassemia / Q000150:complications / Q000453:epidemiology*
chemical_list: D018829:Adhesins, Bacterial; D001427:Bacterial Toxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D022622:Shiga Toxin 1; D022641:Shiga Toxin 2; C034151:heat stable toxin (E coli); C073819:eaeA protein, E coli; C027538:heat-labile enterotoxin, E coli
keywords: Escherichia coli; HIV; thalassemia