title: Endothelial Ca 2+ oscillations reflect VEGFR signaling-regulated angiogenic capacity in vivo.
abstract: Sprouting angiogenesis is a well-coordinated process controlled by multiple extracellular inputs, including vascular endothelial growth factor (VEGF). However, little is known about when and how individual endothelial cell (EC) responds to angiogenic inputs in vivo. Here, we visualized endothelial Ca(2+) dynamics in zebrafish and found that intracellular Ca(2+) oscillations occurred in ECs exhibiting angiogenic behavior. Ca(2+) oscillations depended upon VEGF receptor-2 (Vegfr2) and Vegfr3 in ECs budding from the dorsal aorta (DA) and posterior cardinal vein, respectively. Thus, visualizing Ca(2+) oscillations allowed us to monitor EC responses to angiogenic cues. Vegfr-dependent Ca(2+) oscillations occurred in migrating tip cells as well as stalk cells budding from the DA. We investigated how Dll4/Notch signaling regulates endothelial Ca(2+) oscillations and found that it was required for the selection of single stalk cell as well as tip cell. Thus, we captured spatio-temporal Ca(2+) dynamics during sprouting angiogenesis, as a result of cellular responses to angiogenic inputs.
authors.affiliation: Department of Cell Biology, National Cerebral and Cardiovascular Center Research Institute, Suita, Japan.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Yokota
authors.affiliation: Department of Cell Biology, National Cerebral and Cardiovascular Center Research Institute, Suita, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Nakajima
authors.affiliation: Department of Cell Biology, National Cerebral and Cardiovascular Center Research Institute, Suita, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Wakayama
authors.affiliation: Division of Molecular and Developmental Biology, National Institute of Genetics, Mishima, Japan.|Department of Genetics, SOKENDAI (The Graduate University for Advanced Studies), National Institute of Genetics, Mishima, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Muto
authors.affiliation: Division of Molecular and Developmental Biology, National Institute of Genetics, Mishima, Japan.|Department of Genetics, SOKENDAI (The Graduate University for Advanced Studies), National Institute of Genetics, Mishima, Japan.
authors.forename: Koichi
authors.initials: K
authors.lastname: Kawakami
authors.affiliation: Department of Cell Biology, National Cerebral and Cardiovascular Center Research Institute, Suita, Japan.
authors.forename: Shigetomo
authors.initials: S
authors.lastname: Fukuhara
authors.affiliation: Department of Cell Biology, National Cerebral and Cardiovascular Center Research Institute, Suita, Japan.|AMED-CREST, Japan Agency for Medical Research and Development, Suita, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Mochizuki
mesh_terms: D000818:Animals; D020013:Calcium Signaling; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000502:physiology*; D018919:Neovascularization, Physiologic; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000378:metabolism*; D040321:Vascular Endothelial Growth Factor Receptor-3 / Q000378:metabolism*; D015027:Zebrafish
chemical_list: D040301:Vascular Endothelial Growth Factor Receptor-2; D040321:Vascular Endothelial Growth Factor Receptor-3
keywords: VEGF; angiogenesis; calcium; developmental biology; imaging; stem cells; zebrafish