title: Functional properties of in vitro excitatory cortical neurons derived from human pluripotent stem cells.
abstract: The in vitro derivation of regionally defined human neuron types from patient-derived stem cells is now established as a resource to investigate human development and disease. Characterization of such neurons initially focused on the expression of developmentally regulated transcription factors and neural markers, in conjunction with the development of protocols to direct and chart the fate of differentiated neurons. However, crucial to the understanding and exploitation of this technology is to determine the degree to which neurons recapitulate the key functional features exhibited by their native counterparts, essential for determining their usefulness in modelling human physiology and disease in vitro. Here, we review the emerging data concerning functional properties of human pluripotent stem cell-derived excitatory cortical neurons, in the context of both maturation and regional specificity.
authors.affiliation: Centre for Integrative Physiology, University of Edinburgh, Edinburgh, EH8 9XD, UK.|Euan MacDonald Centre for MND Research, University of Edinburgh, Edinburgh, EH16 4SB, UK.
authors.forename: Matthew R
authors.initials: MR
authors.lastname: Livesey
authors.affiliation: Euan MacDonald Centre for MND Research, University of Edinburgh, Edinburgh, EH16 4SB, UK.|Centre for Clinical Brain Sciences, University of Edinburgh, Edinburgh, EH16 4SB, UK.|MRC Centre for Regenerative Medicine, University of Edinburgh, Edinburgh, EH16 4SB, UK.
authors.forename: Dario
authors.initials: D
authors.lastname: Magnani
authors.affiliation: Centre for Integrative Physiology, University of Edinburgh, Edinburgh, EH8 9XD, UK.|Euan MacDonald Centre for MND Research, University of Edinburgh, Edinburgh, EH16 4SB, UK.
authors.forename: Giles E
authors.initials: GE
authors.lastname: Hardingham
authors.affiliation: Euan MacDonald Centre for MND Research, University of Edinburgh, Edinburgh, EH16 4SB, UK.|Centre for Clinical Brain Sciences, University of Edinburgh, Edinburgh, EH16 4SB, UK.|MRC Centre for Regenerative Medicine, University of Edinburgh, Edinburgh, EH16 4SB, UK.|Centre for Brain Development and Repair, Institute for Stem Cell Biology and Regenerative Medicine, Bangalore, 560065, India.
authors.forename: Siddharthan
authors.initials: S
authors.lastname: Chandran
authors.affiliation: Centre for Integrative Physiology, University of Edinburgh, Edinburgh, EH8 9XD, UK.|Euan MacDonald Centre for MND Research, University of Edinburgh, Edinburgh, EH16 4SB, UK.|Centre for Brain Development and Repair, Institute for Stem Cell Biology and Regenerative Medicine, Bangalore, 560065, India.
authors.forename: David J A
authors.initials: DJ
authors.lastname: Wyllie
mesh_terms: D000200:Action Potentials / Q000502:physiology*; D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D006801:Humans; D009474:Neurons; D039904:Pluripotent Stem Cells / Q000378:metabolism / Q000502:physiology*; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D014157:Transcription Factors