title: Synergistic action of histone acetyltransferase GCN5 and receptor CLAVATA1 negatively affects ethylene responses in Arabidopsis thaliana.
abstract: GENERAL CONTROL NON-REPRESSIBLE 5 (GCN5) is a histone acetyltransferase (HAT) and the catalytic subunit of several multicomponent HAT complexes that acetylate lysine residues of histone H3. Mutants in AtGCN5 display pleiotropic developmental defects including aberrant meristem function. Shoot apical meristem (SAM) maintenance is regulated by CLAVATA1 (CLV1), a receptor kinase that controls the size of the shoot and floral meristems. Upon activation through CLV3 binding, CLV1 signals to the transcription factor WUSCHEL (WUS), restricting WUS expression and thus the meristem size. We hypothesized that GCN5 and CLV1 act together to affect SAM function. Using genetic and molecular approaches, we generated and characterized clv gcn5 mutants. Surprisingly, the clv1-1 gcn5-1 double mutant exhibited constitutive ethylene responses, suggesting that GCN5 and CLV signaling act synergistically to inhibit ethylene responses in Arabidopsis. This genetic and molecular interaction was mediated by ETHYLENE INSENSITIVE 3/ EIN3-LIKE1 (EIN3/EIL1) transcription factors. Our data suggest that signals from the CLV transduction pathway reach the GCN5-containing complexes in the nucleus and alter the histone acetylation status of ethylene-responsive genes, thus translating the CLV information to transcriptional activity and uncovering a link between histone acetylation and SAM maintenance in the complex mode of ethylene signaling.
authors.affiliation: Department of Botany, School of Biology, Faculty of Science, Aristotle University of Thessaloniki, Thessaloniki 54124, Greece.
authors.forename: Stylianos
authors.initials: S
authors.lastname: Poulios
authors.affiliation: Department of Botany, School of Biology, Faculty of Science, Aristotle University of Thessaloniki, Thessaloniki 54124, Greece kvlachon@bio.auth.gr.
authors.forename: Konstantinos E
authors.initials: KE
authors.lastname: Vlachonasios
mesh_terms: D000107:Acetylation / Q000187:drug effects; D017360:Arabidopsis / Q000187:drug effects* / Q000201:enzymology* / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D004843:Epistasis, Genetic / Q000187:drug effects; D005030:Ethylenes / Q000494:pharmacology*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant; D051548:Histone Acetyltransferases / Q000235:genetics / Q000378:metabolism*; D006657:Histones / Q000378:metabolism; D007210:Indoleacetic Acids / Q000494:pharmacology; D008954:Models, Biological; D009154:Mutation / Q000235:genetics; D010641:Phenotype; D017346:Protein Serine-Threonine Kinases; D020794:Receptor Protein-Tyrosine Kinases / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects; D014158:Transcription, Genetic / Q000187:drug effects; D000099224:p300-CBP-Associated Factor
chemical_list: D029681:Arabidopsis Proteins; D005030:Ethylenes; D051548:Histone Acetyltransferases; D006657:Histones; D007210:Indoleacetic Acids; D017346:Protein Serine-Threonine Kinases; D020794:Receptor Protein-Tyrosine Kinases; C036216:ethylene; C474636:GCN5 protein, Arabidopsis; D000099224:p300-CBP-Associated Factor; C118166:CLV1 protein, Arabidopsis
keywords: Arabidopsis; CLV1; EIN3; GCN5; auxin; ethylene; histone acetylation; triple response.