title: Identification of Candidate Genes for Seed Glucosinolate Content Using Association Mapping in Brassica napus L.
abstract: Rapeseed contains glucosinolates, a toxic group of sulfur-containing glucosides, which play critical roles in defense against herbivores and microbes. However, the presence of glucosinolates in rapeseed reduces the value of the meal as feed for livestock. We performed association mapping of seed glucosinolate (GS) content using the 60K Brassica Infinium single nucleotide polymorphism (SNP) array in 520 oilseed rape accessions. A total of 11 peak SNPs significantly associated with GS content were detected in growing seasons of 2013 and 2014 and were located on B. napus chromosomes A08, A09, C03, and C09, respectively. Two associated regions of GS content covered by these markers were further verified, and three B. napus homologous genes involved in the biosynthesis and accumulation of GS were identified. These genes were multigene family members and were distributed on different chromosomes. Moreover, two genes (BnGRT2 and BnMYB28) associated with GS content were validated by the qRT-PCR analysis of their expression profiles. The further identification and functionalization of these genes will provide useful insight into the mechanism underlying GS biosynthesis and allocation in B. napus, and the associated SNPs markers could be helpful for molecular maker-assisted breeding for low seed GS in B. napus.
authors.affiliation: Chongqing Engineering Research Center for Rapeseed, College of Agronomy and Biotechnology, Southwest University, Tiansheng Road 2, Beibei, Chongqing 400716, China. qucunmin@swu.edu.cn.|Engineering Research Center of South Upland Agriculture of Ministry of Education, Southwest University, Beibei, Chongqing 400716, China. qucunmin@swu.edu.cn.|Food and Bioproduct Science, University of Saskatchewan, 51 Campus Drive, Saskatoon, SK S7N 5A8, Canada. qucunmin@swu.edu.cn.
authors.forename: Cun-Min
authors.initials: CM
authors.lastname: Qu
authors.affiliation: Chongqing Engineering Research Center for Rapeseed, College of Agronomy and Biotechnology, Southwest University, Tiansheng Road 2, Beibei, Chongqing 400716, China. li_shimeng90@163.com.|Engineering Research Center of South Upland Agriculture of Ministry of Education, Southwest University, Beibei, Chongqing 400716, China. li_shimeng90@163.com.
authors.forename: Shi-Meng
authors.initials: SM
authors.lastname: Li
authors.affiliation: Chongqing Engineering Research Center for Rapeseed, College of Agronomy and Biotechnology, Southwest University, Tiansheng Road 2, Beibei, Chongqing 400716, China. duanxiujian9077@163.com.|Engineering Research Center of South Upland Agriculture of Ministry of Education, Southwest University, Beibei, Chongqing 400716, China. duanxiujian9077@163.com.
authors.forename: Xiu-Jian
authors.initials: XJ
authors.lastname: Duan
authors.affiliation: Chongqing Engineering Research Center for Rapeseed, College of Agronomy and Biotechnology, Southwest University, Tiansheng Road 2, Beibei, Chongqing 400716, China. lingfeng9511@126.com.|Engineering Research Center of South Upland Agriculture of Ministry of Education, Southwest University, Beibei, Chongqing 400716, China. lingfeng9511@126.com.
authors.forename: Jin-Hua
authors.initials: JH
authors.lastname: Fan
authors.affiliation: Chongqing Engineering Research Center for Rapeseed, College of Agronomy and Biotechnology, Southwest University, Tiansheng Road 2, Beibei, Chongqing 400716, China. jiald2014@foxmail.com.|Engineering Research Center of South Upland Agriculture of Ministry of Education, Southwest University, Beibei, Chongqing 400716, China. jiald2014@foxmail.com.
authors.forename: Le-Dong
authors.initials: LD
authors.lastname: Jia
authors.affiliation: Chongqing Engineering Research Center for Rapeseed, College of Agronomy and Biotechnology, Southwest University, Tiansheng Road 2, Beibei, Chongqing 400716, China. 13639767407@163.com.|Engineering Research Center of South Upland Agriculture of Ministry of Education, Southwest University, Beibei, Chongqing 400716, China. 13639767407@163.com.
authors.forename: Hui-Yan
authors.initials: HY
authors.lastname: Zhao
authors.affiliation: Chongqing Engineering Research Center for Rapeseed, College of Agronomy and Biotechnology, Southwest University, Tiansheng Road 2, Beibei, Chongqing 400716, China. drlukun@swu.edu.cn.|Engineering Research Center of South Upland Agriculture of Ministry of Education, Southwest University, Beibei, Chongqing 400716, China. drlukun@swu.edu.cn.
authors.forename: Kun
authors.initials: K
authors.lastname: Lu
authors.affiliation: Chongqing Engineering Research Center for Rapeseed, College of Agronomy and Biotechnology, Southwest University, Tiansheng Road 2, Beibei, Chongqing 400716, China. ljn1950@swu.edu.cn.|Engineering Research Center of South Upland Agriculture of Ministry of Education, Southwest University, Beibei, Chongqing 400716, China. ljn1950@swu.edu.cn.
authors.forename: Jia-Na
authors.initials: JN
authors.lastname: Li
authors.affiliation: Chongqing Engineering Research Center for Rapeseed, College of Agronomy and Biotechnology, Southwest University, Tiansheng Road 2, Beibei, Chongqing 400716, China. xinfuxu@126.com.|Engineering Research Center of South Upland Agriculture of Ministry of Education, Southwest University, Beibei, Chongqing 400716, China. xinfuxu@126.com.
authors.forename: Xin-Fu
authors.initials: XF
authors.lastname: Xu
authors.affiliation: Chongqing Engineering Research Center for Rapeseed, College of Agronomy and Biotechnology, Southwest University, Tiansheng Road 2, Beibei, Chongqing 400716, China. ruiwang71@163.com.|Engineering Research Center of South Upland Agriculture of Ministry of Education, Southwest University, Beibei, Chongqing 400716, China. ruiwang71@163.com.
authors.forename: Rui
authors.initials: R
authors.lastname: Wang
keywords: Brassica napus L.; association mapping; seed glucosinolate (GS) content; single nucleotide polymorphism (SNP)