title: The involvement of hematopoietic pre-B cell leukemia transcription factor-interacting protein in regulating epithelial-mesenchymal transition of human spinal glioblastoma.
abstract: To date, hematopoietic pre-B cell leukemia transcription factor-interacting protein (HPIP), a co-repressor for the transcription factor PBX, has been involved into the initiation and onset in a wide variety of cancers. However, the molecular mechanisms underlying HPIP-induced epithelial-mesenchymal transition (EMT) in the spinal glioblastoma have been under investigation. In the present study, spinal glioblastoma tissues, U87, and U251 cell lines were used and subjected to in vitro assays, such as RT-PCR, and Western blot. Here, in vitro assays revealed that HPIP mRNA and protein were highly expressed in five cases of spinal glioblastoma tissues, compared with non-tumor tissues. Subsequently, in vitro experiments demonstrated HPIP promoted the U87 and U251 cell growth and regulated the G1/S phase transitions in U87 and U251 cell cycle, respectively, accompanied by the increased expression of cyclin A2, cyclin B1, and cyclin D1. Furthermore, HPIP increased the expression of N-cadherin, Slug, and MMP2, and decreased the expression of E-cadherin. By contrast, knockdown of HPIP reversed HPIP-induced EMT biomarkers, migration, and invasion in U87 and U251 cells. In conclusion, our findings identified HPIP plays an important role in the progression and EMT of spinal glioblastoma, by which cell growth is improved. Thus, HPIP gene or protein could act as a useful target in the clinical practice.
authors.affiliation: Department of Neurosurgery, Shandong University Hospital of Traditional Chinese Medicine, No.16369, Jingshi Road, Jinan, 250014, Shandong, China.
authors.forename: Deliang
authors.initials: D
authors.lastname: Wang
authors.affiliation: Department of Gynaecology, Shandong University Hospital of Traditional Chinese Medicine, Jinan, 250014, Shandong, China.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, Shandong University Hospital of Traditional Chinese Medicine, No.16369, Jingshi Road, Jinan, 250014, Shandong, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Department of Neurosurgery, Shandong University Hospital of Traditional Chinese Medicine, No.16369, Jingshi Road, Jinan, 250014, Shandong, China.
authors.forename: Xinjun
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Department of Neurosurgery, Shandong University Hospital of Traditional Chinese Medicine, No.16369, Jingshi Road, Jinan, 250014, Shandong, China. xionghtcm@126.com.
authors.forename: Hui
authors.initials: H
authors.lastname: Xiong
mesh_terms: D000293:Adolescent; D000328:Adult; D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D056970:Co-Repressor Proteins; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics*; D005260:Female; D015870:Gene Expression; D055785:Gene Knockdown Techniques; D005909:Glioblastoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D008279:Magnetic Resonance Imaging; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D013125:Spinal Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D055815:Young Adult
chemical_list: D056970:Co-Repressor Proteins; C413449:PBXIP1 protein, human; D014157:Transcription Factors
keywords: EMT; Glioblastoma; HPIP; Regulation