title: Extraction, partial purification and determination of some biochemical properties of β-glucosidase from Tea Leaves (Camellia sinensis L.).
abstract: This research was carried out to determine biochemical properties of β-glucosidase (β-D-glucoside glucohydrolase, EC 3.2.1.21) isolated from Turkish tea leaves. Two protein peaks containing β-glucosidase activity were recovered and characterized, which were denoted as isoenzyme A and isoenzyme B. Their pH optimum, thermal resistances, affinity towards p-nitrophenyl-β-D-glucopyranoside differed markedly. They both displayed maximal activity at pH 5.0. The effects of the inhibitors tested varied in a dose dependent manner.
authors.affiliation: Faculty of Engineering, Department of Food Engineering, Altinsehir, 02040 Adiyaman, Turkey.
authors.forename: Aysun
authors.initials: A
authors.lastname: Şener
keywords: Kinetics; Purification; Tea leaves; Thermal inactivation; Thermal stability; β-glucosidase