title: Glutathione production by recombinant Escherichia coli expressing bifunctional glutathione synthetase.
abstract: Glutathione (GSH) is an important bioactive substance applied widely in pharmaceutical and food industries. Due to the strong product inhibition in the GSH biosynthetic pathway, high levels of intracellular content, yield and productivity of GSH are difficult to achieve. Recently, a novel bifunctional GSH synthetase was identified to be less sensitive to GSH. A recombinant Escherichia coli strain expressing gshF encoding the bifunctional glutathione synthetase of Streptococcus thermophilus was constructed for GSH production. In this study, efficient GSH production using this engineered strain was investigated. The cultivation process was optimized by controlling dissolved oxygen (DO), amino acid addition and glucose feeding. 36.8 mM (11.3 g/L) GSH were formed at a productivity of 2.06 mM/h when the amino acid precursors (75 mM each) were added and glucose was supplied as the sole carbon and energy source.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, 200237, Shanghai, China.
authors.forename: Dezheng
authors.initials: D
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, 200237, Shanghai, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, 200237, Shanghai, China. hwu@ecust.edu.cn.
authors.forename: Hui
authors.initials: H
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, 200237, Shanghai, China. lizm@ecust.edu.cn.|Shanghai Collaborative Innovation Center for Biomanufacturing Technology, 130 Meilong Road, 200237, Shanghai, China. lizm@ecust.edu.cn.
authors.forename: Zhimin
authors.initials: Z
authors.lastname: Li
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, 200237, Shanghai, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Ye
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D053898:Biosynthetic Pathways; D004734:Energy Metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D005978:Glutathione / Q000096:biosynthesis*; D005981:Glutathione Synthase / Q000235:genetics / Q000378:metabolism*; D064251:Multifunctional Enzymes / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism; D048249:Streptococcus thermophilus / Q000201:enzymology
chemical_list: D000596:Amino Acids; D064251:Multifunctional Enzymes; D005981:Glutathione Synthase; D005978:Glutathione; D005947:Glucose; D010100:Oxygen
keywords: Bifunctional glutathione synthetase; Energy regeneration; Escherichia coli; Glutathione; One-strain system