title: Impact of preformed and de novo anti-HLA DP antibodies in renal allograft survival.
abstract: The influence of antibodies against HLA-DP antigens detected with solid-phase assays on graft survival after kidney transplantation (KT) is uncertain. We evaluated with Luminex® the prevalence of pre- and posttransplant DP antibodies in 440 KT patients and their impact on graft survival. For 291 patients with available pretransplant samples, DP antibodies were present in 39.7% KT with pretransplant HLA antibodies and 47.7% with DSA. Graft survival of KT with pretransplant class-II DSA was worse than with non-DSA (p=0.01). DP antibodies did not influence graft survival. Of 346 patients monitored post-KT, 17.1% had HLA class-II antibodies, 56% with DP antibodies. Class-II DSA was detected in 39%, 60.9% of them had DP antibodies. Graft survival was worse in patients with class-II DSA (p=0.022). DP antibodies did not change these results. The presence of isolated DP antibodies was a rare event both pre- and posttransplantation (1.03 and 0.86%). The presence of pretransplant and posttransplant DSA is associated with a negative impact on graft survival. However, the presence of DP antibodies does not modify this impact significantly.
authors.affiliation: Department of Nephrology, Institute Mar for Medical Research, Hospital del Mar, Barcelona, Spain.
authors.forename: Dolores
authors.initials: D
authors.lastname: Redondo-Pachón
authors.affiliation: Department of Nephrology, Institute Mar for Medical Research, Hospital del Mar, Barcelona, Spain. Electronic address: julpascual@gmail.com.
authors.forename: Julio
authors.initials: J
authors.lastname: Pascual
authors.affiliation: Department of Nephrology, Institute Mar for Medical Research, Hospital del Mar, Barcelona, Spain.
authors.forename: María J
authors.initials: MJ
authors.lastname: Pérez-Sáez
authors.affiliation: Laboratori de Referencia de Catalunya, Barcelona, Spain.
authors.forename: Carmen
authors.initials: C
authors.lastname: García
authors.affiliation: Laboratori de Referencia de Catalunya, Barcelona, Spain.
authors.forename: Juan José
authors.initials: JJ
authors.lastname: Hernández
authors.affiliation: Department of Pathology, Hospital del Mar, Barcelona, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: Gimeno
authors.affiliation: Department of Nephrology, Institute Mar for Medical Research, Hospital del Mar, Barcelona, Spain.
authors.forename: Marisa
authors.initials: M
authors.lastname: Mir
authors.affiliation: Department of Nephrology, Institute Mar for Medical Research, Hospital del Mar, Barcelona, Spain.
authors.forename: Marta
authors.initials: M
authors.lastname: Crespo
mesh_terms: D000328:Adult; D000368:Aged; D064591:Allografts / Q000276:immunology; D005260:Female; D006084:Graft Rejection / Q000276:immunology*; D006085:Graft Survival / Q000276:immunology; D006682:HLA-DP Antigens / Q000276:immunology / Q000378:metabolism*; D006650:Histocompatibility Testing; D006801:Humans; D007518:Isoantibodies / Q000097:blood; D007668:Kidney / Q000276:immunology; D016030:Kidney Transplantation; D008297:Male; D008875:Middle Aged; D014184:Transplantation, Homologous
chemical_list: D006682:HLA-DP Antigens; D007518:Isoantibodies
keywords: Graft survival; HLA-DP antibodies; Renal transplant