title: RETRACTED: pNaKtide inhibits Na/K-ATPase reactive oxygen species amplification and attenuates adipogenesis.
abstract: Obesity has become a worldwide epidemic and is a major risk factor for metabolic syndrome. Oxidative stress is known to play a role in the generation and maintenance of an obesity phenotype in both isolated adipocytes and intact animals. Because we had identified that the Na/K-ATPase can amplify oxidant signaling, we speculated that a peptide designed to inhibit this pathway, pNaKtide, might ameliorate an obesity phenotype. To test this hypothesis, we first performed studies in isolated murine preadipocytes (3T3L1 cells) and found that pNaKtide attenuated oxidant stress and lipid accumulation in a dose-dependent manner. Complementary experiments in C57Bl6 mice fed a high-fat diet corroborated our in vitro observations. Administration of pNaKtide in these mice reduced body weight gain, restored systemic redox and inflammatory milieu, and, crucially, improved insulin sensitivity. Thus, we propose that inhibition of Na/K-ATPase amplification of oxidative stress may ultimately be a novel way to combat obesity, insulin resistance, and metabolic syndrome.
authors.affiliation: Departments of Medicine, Pharmacology, and Surgery, Joan C. Edwards School of Medicine, Marshall University, Huntington, WV 25701, USA.
authors.forename: Komal
authors.initials: K
authors.lastname: Sodhi
authors.affiliation: Departments of Medicine, Pharmacology, and Surgery, Joan C. Edwards School of Medicine, Marshall University, Huntington, WV 25701, USA.
authors.forename: Kyle
authors.initials: K
authors.lastname: Maxwell
authors.affiliation: Departments of Medicine, Pharmacology, and Surgery, Joan C. Edwards School of Medicine, Marshall University, Huntington, WV 25701, USA.
authors.forename: Yanling
authors.initials: Y
authors.lastname: Yan
authors.affiliation: Departments of Medicine, Pharmacology, and Surgery, Joan C. Edwards School of Medicine, Marshall University, Huntington, WV 25701, USA.
authors.forename: Jiang
authors.initials: J
authors.lastname: Liu
authors.affiliation: Departments of Medicine, Pharmacology, and Surgery, Joan C. Edwards School of Medicine, Marshall University, Huntington, WV 25701, USA.
authors.forename: Muhammad A
authors.initials: MA
authors.lastname: Chaudhry
authors.affiliation: Departments of Medicine, Pharmacology, and Surgery, Joan C. Edwards School of Medicine, Marshall University, Huntington, WV 25701, USA.
authors.forename: Morghan
authors.initials: M
authors.lastname: Getty
authors.affiliation: Departments of Medicine, Pharmacology, and Surgery, Joan C. Edwards School of Medicine, Marshall University, Huntington, WV 25701, USA.
authors.forename: Zijian
authors.initials: Z
authors.lastname: Xie
authors.affiliation: Departments of Medicine, Pharmacology, and Surgery, Joan C. Edwards School of Medicine, Marshall University, Huntington, WV 25701, USA. ; Department of Medicine, New York Medical College, Valhalla, NY 10595, USA.
authors.forename: Nader G
authors.initials: NG
authors.lastname: Abraham
authors.affiliation: Departments of Medicine, Pharmacology, and Surgery, Joan C. Edwards School of Medicine, Marshall University, Huntington, WV 25701, USA.
authors.forename: Joseph I
authors.initials: JI
authors.lastname: Shapiro
keywords: Na/K-ATPase; adipogenesis; high-fat diet; metabolic syndrome; oxidative stress; pNaKtide peptide