title: Parviterribacter kavangonensisgen. nov., sp. nov. andParviterribacter multiflagellatussp. nov., novel members ofParviterribacteraceaefam. nov. within the orderSolirubrobacterales, and emended descriptions of the classesThermoleophiliaandRubrobacteriaand their orders and families.
abstract: Two Gram-type-positive, non-spore-forming bacteria, strains D16/0/H6Tand A22/0/F9_1T, were isolated from Namibian semiarid savannah soils. 16S rRNA gene sequence analysis revealed 96.6 % identity between the two strains and placed them within the orderSolirubrobacteralesof the classThermoleophilia. The closest phylogenetic relatives with validly published names were several strains of the genusSolirubrobacterand the speciesConexibacter arvalis, with pairwise sequence similarities of ≤ 94.0 %. Cells of strain D16/0/H6Twere ovoid to rod-shaped, whereas strain A22/0/F9_1Tformed regular rods. Cells of both strains were motile and divided by binary fission. Colonies were pink and white to pale yellowish/brownish, respectively. Strains D16/0/H6Tand A22/0/F9_1Twere aerobic, chemoheterotrophic mesophiles with broad temperature (13-43 and 17-43 °C, respectively) and pH (pH 4.5-8.5 and 5.0-9.5) ranges for growth. Complex proteinaceous substrates and glucose were the preferred carbon and energy sources. Strain A22/0/F9_1Talso grew on various carboxylic acids. For both strains, the peptidoglycan diamino acid wasmeso-2,6-diaminopimelic acid. The major quinone was MK-8. As a minor compound, MK-7 occurred in strain D16/0/H6T; strain A22/0F9_1Talso contained MK-7, MK-7(H2) and MK-8(H2). Major fatty acids of strain D16/0/H6Twere 10-methyl C17 : 0, iso-C16 : 0and C18 : 1ω9c. Strain A22/0F9_1Tcontained C18 : 1ω9c, C17 : 1ω8c, C17 : 1ω6cand iso-C16 : 0as major components. The DNA G+C contents of strains D16/0/H6Tand A22/0/F9_1Twere 72.8 and 74.0 mol%, respectively. Based on these characteristics, the two isolates are assigned to novel species of the new genusParviterribactergen. nov., the type speciesParviterribacter kavangonensissp. nov. (type strain D16/0/H6T= DSM 25205T= LMG 26950T) and a second speciesParviterribacter multiflagellatussp. nov. (type strain A22/0/F9_1T= DSM 25204T= LMG 26949T). As the novel genus and species cannot be clearly assigned to an established family within the orderSolirubrobacterales, the novel familyParviterribacteraceaefam. nov. is proposed. Emended descriptions of the classesThermoleophiliaandRubrobacteriaand their orders and families are also provided.
authors.affiliation: Department of Microbial Ecology and Diversity Research, Leibniz Institut DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen, Braunschweig, Germany.
authors.forename: Bärbel U
authors.initials: BU
authors.lastname: Foesel
authors.affiliation: Department of Microbial Ecology and Diversity Research, Leibniz Institut DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen, Braunschweig, Germany.
authors.forename: Alicia
authors.initials: A
authors.lastname: Geppert
authors.affiliation: Central Facility for Microscopy, Helmholtz Centre for Infection Research, Braunschweig, Germany.
authors.forename: Manfred
authors.initials: M
authors.lastname: Rohde
authors.affiliation: Department of Microbial Ecology and Diversity Research, Leibniz Institut DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen, Braunschweig, Germany.|Technische Universität Braunschweig, Braunschweig, Germany.
authors.forename: Jörg
authors.initials: J
authors.lastname: Overmann