title: Oxygen stress: impact on innate immune system, antioxidant defence system and expression of HIF-1α and ATPase 6 genes in Catla catla.
abstract: Catla catla catla (2.28 ± 0.1 g) were exposed to six different levels of dissolved oxygen: 1 (DO-1), 3 (DO-3), 5 (DO-5), 7 (DO-7), 9 (DO-9) and 11 (DO-11) mg/L. DO-5 served as control. In DO-1 and DO-3, the number of red blood cells (RBC), lysozyme, respiratory burst activity and nitric oxide synthase were significantly (p < 0.05) lower compared to the control one. In DO-7 and DO-9, RBC and lysozyme were significantly (p < 0.05) higher compared to the control one. Thiobarbituric acid reactive substances was significantly (p < 0.05) higher in catla exposed at low (1 and 3 mg/L) and high (9 and 11 mg/L) dissolved oxygen compared to others. In muscles and hepatopancreas, reduced glutathione was significantly (p < 0.05) higher in DO-5 and DO-7 and in gills of DO-5 compared to others after 1 h. In muscles, glutathione S-transferase (GST) was significantly (p < 0.05) lower in DO-5 and DO-7 compared to others. In hepatopancreas, GST and glutathione peroxidise (GPx) were significantly (p < 0.05) higher in DO-1 and DO-3 compared to others. In gills, GPx was higher in DO-9 and DO-11 after 48 h. In brain, hypoxia-inducible factor (HIF)-1α mRNA level was induced in DO-1 and DO-3 compared to others after 1 h of exposure. In gills and hepatopancreas, HIF-1α mRNA level was significantly (p < 0.05) higher in DO-1 compared to others after 1 h. The ATPase 6 mRNA level was significantly (p < 0.05) higher in brain and hepatopancreas of DO-1 after 1 h and in gills and hepatopancreas of DO-3 and DO-9, respectively, after 48 h compared to others.
authors.affiliation: Aqua Research Lab, Department of Zoology, University of Delhi, Delhi, 110007, India.
authors.forename: Samar Pal
authors.initials: SP
authors.lastname: Singh
authors.affiliation: Department of Biotechnology, Delhi Technological University, Delhi, 110042, India.
authors.forename: JaiGopal
authors.initials: J
authors.lastname: Sharma
authors.affiliation: Aqua Research Lab, Department of Zoology, University of Delhi, Delhi, 110007, India.
authors.forename: Tauqueer
authors.initials: T
authors.lastname: Ahmad
authors.affiliation: Aqua Research Lab, Department of Zoology, University of Delhi, Delhi, 110007, India. aquaresearchlab@yahoo.co.in.
authors.forename: Rina
authors.initials: R
authors.lastname: Chakrabarti
mesh_terms: D000818:Animals; D003530:Cyprinidae / Q000276:immunology / Q000502:physiology*; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics* / Q000378:metabolism; D007107:Immune System; D007113:Immunity, Innate / Q000502:physiology*; D025261:Mitochondrial Proton-Translocating ATPases / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger; D013312:Stress, Physiological / Q000502:physiology*
chemical_list: D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D012333:RNA, Messenger; D025261:Mitochondrial Proton-Translocating ATPases
keywords: ATPase 6; Antioxidant enzymes; Catla catla; Hypoxia-inducible factor (HIF)-1α; Lysozyme; Nitric oxide synthase