title: Ultrasensitive Cytosensor Based on Self-Enhanced Electrochemiluminescent Ruthenium-Silica Composite Nanoparticles for Efficient Drug Screening with Cell Apoptosis Monitoring.
abstract: The self-enhanced electrochemiluminescence (ECL) with high sensitivity could be an effective method for anticancer drug screening with cell apoptosis monitoring. Here we reported an ultrasensitive ECL cytosensor for cell apoptosis monitoring by using self-enhanced electrochemiluminescent ruthenium-silica composite nanoparticles (Ru-N-SiNPs) labeled annexin V as signal probes. The Ru-N-SiNPs were first synthesized through simple hydrolysis of a novel precursor containing luminescent and intracoreactant groups in one molecule, which presented higher emission efficiency and enhanced ECL intensity due to the shorter electron-transfer path and less energy loss. Moreover, the as-proposed ECL cytosensor was successfully used to investigate efficiency of paclitaxel toward MDA-MB-231 breast cancer cell in the range from 1 nM to 200 nM with a detection limit of 0.3 nM and a correlation coefficient of 0.9917. The improved accuracy and excellent dynamic range revealed the potential applications in biomolecules diagnostics and cells detections, especially in living and complex systems.
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University , Chongqing 400715, PR China.|Department of Clinical Biochemistry, Laboratory Sciences, Southwest Hospital, Third Military Medical University , 30 Gaotanyan Street, Shapingba District, Chongqing 400038, PR China.
authors.forename: Wenbin
authors.initials: W
authors.lastname: Liang
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University , Chongqing 400715, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhuo
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University , Chongqing 400715, PR China.
authors.forename: Chengyi
authors.initials: C
authors.lastname: Xiong
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University , Chongqing 400715, PR China.
authors.forename: Yingning
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University , Chongqing 400715, PR China.
authors.forename: Yaqin
authors.initials: Y
authors.lastname: Chai
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University , Chongqing 400715, PR China.
authors.forename: Ruo
authors.initials: R
authors.lastname: Yuan
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D015374:Biosensing Techniques; D001943:Breast Neoplasms / Q000188:drug therapy / Q000473:pathology; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D055664:Electrochemical Techniques; D005260:Female; D006801:Humans; D008163:Luminescent Measurements; D053758:Nanoparticles / Q000737:chemistry*; D012428:Ruthenium / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*; D014407:Tumor Cells, Cultured
chemical_list: D000970:Antineoplastic Agents; D012822:Silicon Dioxide; D012428:Ruthenium