title: [Inhibitory effects of butyl alcohol extract of Baitouweng decoction on virulence factors of Candida tropicalis].
abstract: OBJECTIVE
  To investigate the effects of butyl alcohol extract of baitouweng decoction (BAEB) on the fungal cell surface hydrophobicity (CSH), filamentation and biofilm formation of Candida tropicalis.
  
  
  METHODS
  Gradual dilution method was used to determine the MIC. XTT assay was applied to determine the SMIC80. Time-Kill assay was employed to draw the Time-Kill curve. The water-hydrocarbon two-phase assay was used to measure the cell surface hydrophobicity. Scanning electron microscopy (SEM) was applied to observe the morphological changes of the biofilm. Confocal laser scanning microscopy (CLSM) was applied to determine the thickness of the biofilm. The quantification real-time PCR (qRT-PCR) was used to detect expression changes of releated genes (UME6, ALST3 and NRG1). result: The MICs of BAEB against C. tropicalis strains are determined as 64-128 mg x L(-1). The SMIC80 s of BAEB against the biofilm of Candida tropicalis strains are determined as 256-512 mg x L(-1). Time-Kill curve results indicate that BAEB has a promise fungicidal effect at 256 and 512 mg x L(-1). SEM results shows that 512 mg x L(-1) BAEB can inhibit the formation of C. tropicalis biofilm on Silicone catheter, and the morphology of biofilm is also affected by BAEB. The thickness of C. tropicalis biofilm is reduced by BAEB according to CLSM results. Furthermore, qRT-PCR results indicate that expression of UME6 and ALST3 are significantly down-regulated by BAEB 256,512 mg x L(-1), and NRG1 is not affected by BAEB.
  
  
  CONCLUSIONS
  BAEB inhibits effectively the CSH, filamentation and biofilm formation of VVC strains of C. tropicalis.
authors.forename: Gui-ming
authors.initials: GM
authors.lastname: Yan
authors.forename: Meng-xiang
authors.initials: MX
authors.lastname: Zhang
authors.forename: Dan
authors.initials: D
authors.lastname: Xia
authors.forename: Ke-qiao
authors.initials: KQ
authors.lastname: Lu
authors.forename: Jing
authors.initials: J
authors.lastname: Shao
authors.forename: Tian-ming
authors.initials: TM
authors.lastname: Wang
authors.forename: Chang-zhong
authors.initials: CZ
authors.lastname: Wang
mesh_terms: D000935:Antifungal Agents / Q000737:chemistry / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects; D041022:Candida tropicalis / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D002177:Candidiasis / Q000382:microbiology; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology*; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects; D006801:Humans; D037521:Virulence Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D000935:Antifungal Agents; D004365:Drugs, Chinese Herbal; D005656:Fungal Proteins; D037521:Virulence Factors