title: Simultaneous determination of residues of dipyrone metabolites in goat tissues by hydrophilic interaction liquid chromatography tandem mass spectrometry.
abstract: A reliable LC-MS/MS method with high sensitivity was developed and validated for the determination of dipyrone (DIP) metabolites in goat muscle, fat, liver, and kidney samples. Analytes were extracted using acetonitrile mixed with ammonia solution. After dehydration and evaporation to dryness, extracts were purified using an Oasis MAX cartridge. Chromatographic separation was performed on a hydrophilic interaction liquid chromatography column. The analytes were then detected using triple-quadrupole mass spectrometry in positive electrospray ionization and multiple reaction monitoring mode. Calibration plots were constructed using matrix-matched standards and showed good linearity. Limits of quantification for 4-methylaminoantipyrine (MAA), 4-formylaminoantipyrine (FAA), and 4-acetylaminoantipyrone (AAA) ranged from 0.4 μg kg(-1) to 6 μg kg(-1), while those for 4-aminoantipyrone (AA) ranged from 10 μg kg(-1) to 125 μg kg(-1) in all tissues. The developed method was successfully applied in the determination of DIP metabolite residues in actual goat tissues.
authors.affiliation: Key Laboratory of Veterinary Drug Safety Evaluation and Residues Research, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China; Qingdao Vland Biotech Group Co., Ltd, Qingdao 266061, China.
authors.forename: Chong
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Veterinary Drug Safety Evaluation and Residues Research, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China. Electronic address: twinzhang@shvri.ac.cn.
authors.forename: Lifang
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Shandong New Hope Liuhe Group Co., Ltd, Qingdao 266061, China.
authors.forename: Suqing
authors.initials: S
authors.lastname: Cao
authors.affiliation: Key Laboratory of Veterinary Drug Safety Evaluation and Residues Research, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China.
authors.forename: Zhaoling
authors.initials: Z
authors.lastname: Jiang
authors.affiliation: Laboratory of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Wu
authors.affiliation: Laboratory of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Yan
authors.affiliation: Key Laboratory of Veterinary Drug Safety Evaluation and Residues Research, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China.
authors.forename: Xiaoxiao
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Laboratory of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Shanxiang
authors.initials: S
authors.lastname: Jiang
authors.affiliation: Key Laboratory of Veterinary Drug Safety Evaluation and Residues Research, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China. Electronic address: fqxue@shvri.ac.cn.
authors.forename: Feiqun
authors.initials: F
authors.lastname: Xue
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D006041:Goats; D057927:Hydrophobic and Hydrophilic Interactions; D053719:Tandem Mass Spectrometry / Q000379:methods*
keywords: Dipyrone; Goat; Hydrophilic interaction liquid chromatography tandem mass spectrometry; Metabolites; Residues; Tissues