title: Enhancing tetrabromobisphenol A biodegradation in river sediment microcosms and understanding the corresponding microbial community.
abstract: In situ remediation of contaminated sediment using microbes is a promising environmental treatment method. This study used bioaugmentation to investigate the biodegradation of tetrabromobisphenol A (TBBPA) in sediment microcosms collected from an electronic-waste recycling site. Treatments included adding possible biodegradation intermediates of TBBPA, including 2,4-dibromophenol (2,4-DBP), 2,4,6-tribromophenol (TBP), and bisphenol A (BPA) as co-substrates. Bioaugmentation was done with Ochrobactrum sp. T (TBBPA-degrader) and a mixed culture of Ochrobactrum sp. T, Bacillus sp. GZT (TBP-degrader) and Bacillus sp. GZB (BPA-degrader). Results showed that bioaugmentation with Ochrobactrum sp. T significantly improved TBBPA degradation efficiencies in sediment microcosms (P < 0.01); aerobic conditions increased the microbes' degradation activities. Co-substrates 2,4-DBP, TBP and BPA inhibited biodegradation of TBBPA. A metagenomic analysis of total 16S rRNA genes from the treated sediment microcosms showed that the following dominant genera: Ochrobactrum, Parasegetibacter, Thermithiobacillus, Phenylobacterium and Sphingomonas. The genus level of Ochrobactrum increased with increased degradation time, within 10-week of incubation. Microbes from genus Ochrobactrum are mainly linked to enhance the TBBPA biodegradation.
authors.affiliation: State Key Laboratory of Organic Geochemistry and Guangdong Key Laboratory of Environmental Resources Utilization and Protection, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China.
authors.forename: Guiying
authors.initials: G
authors.lastname: Li
authors.affiliation: State Key Laboratory of Organic Geochemistry and Guangdong Key Laboratory of Environmental Resources Utilization and Protection, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Jukun
authors.initials: J
authors.lastname: Xiong
authors.affiliation: School of Life Sciences, The Chinese University of Hong Kong, Shatin, NT, Hong Kong Special Administrative Region.
authors.forename: Po Keung
authors.initials: PK
authors.lastname: Wong
authors.affiliation: State Key Laboratory of Organic Geochemistry and Guangdong Key Laboratory of Environmental Resources Utilization and Protection, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China. Electronic address: antc99@gig.ac.cn.
authors.forename: Taicheng
authors.initials: T
authors.lastname: An
mesh_terms: D001407:Bacillus / Q000378:metabolism; D001559:Benzhydryl Compounds / Q000737:chemistry; D001673:Biodegradation, Environmental / Q000187:drug effects; D044822:Biodiversity; D059029:Electronic Waste / Q000032:analysis; D019015:Geologic Sediments / Q000737:chemistry* / Q000382:microbiology; D042041:Ochrobactrum / Q000378:metabolism; D010636:Phenols / Q000737:chemistry; D011075:Polybrominated Biphenyls / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S; D045483:Rivers / Q000737:chemistry / Q000382:microbiology*; D020578:Sphingomonas; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D010636:Phenols; D011075:Polybrominated Biphenyls; D012336:RNA, Ribosomal, 16S; C020806:tetrabromobisphenol A; C013930:2,4-dibromophenol; C006780:bisphenol A; D000098826:Bisphenol A Compounds; C004554:2,4,6-tribromophenol
keywords: Bioaugmentation; Brominated flame retardants; Microbial community evolution; Ochrobactrum sp. T