title: Three supplementary methods for analyzing cytotoxicity of Escherichia coli O157:H7.
abstract: Escherichia coli O157:H7 is an enterohemorrhagic E. coli (EHEC) strain and a major food-borne pathogen, causing severe disease in humans worldwide. Multiple sensitive, accurate, and quantitative methods are needed to provide a comprehensive analysis of cell damage caused by O157:H7. However, the current, universally adopted methods for O157:H7 virulence assessment fail to investigate the interactive effects of O157:H7 and its host cells, neglect the effects of infection of host cells by O157:H7, and fail to comprehensively and accurately reflect the true pathogenicity of O157:H7. In this study, three different accurate, sensitive, and quantifiable methods were supplementary to provide standard operating procedures to analyze the cytotoxicity of O157:H7. This set of methods can be applied to toxicity studies of newly discovered O157:H7 clinical isolates and used to study how a clinical isolate's toxicity correlates with its pathogenicity. These methods can also be used in future studies of latent virulence factors and to explore the pathogenic mechanisms of O157:H7.
authors.affiliation: Beijing Institute of Biotechnology, Beijing 100071, China; School of Life Sciences, Anhui University, Hefei 230601, Anhui, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Beijing Institute of Biotechnology, Beijing 100071, China.
authors.forename: Yuxia
authors.initials: Y
authors.lastname: Li
authors.affiliation: Beijing Institute of Biotechnology, Beijing 100071, China.
authors.forename: Bingjuan
authors.initials: B
authors.lastname: Li
authors.affiliation: Beijing Institute of Biotechnology, Beijing 100071, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Mao
authors.affiliation: Beijing Institute of Biotechnology, Beijing 100071, China.
authors.forename: Xun
authors.initials: X
authors.lastname: Wu
authors.affiliation: Beijing Institute of Biotechnology, Beijing 100071, China.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Zou
authors.affiliation: Beijing Institute of Biotechnology, Beijing 100071, China; School of Life Sciences, Anhui University, Hefei 230601, Anhui, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Gao
authors.affiliation: Beijing Institute of Biotechnology, Beijing 100071, China.
authors.forename: Hexin
authors.initials: H
authors.lastname: Yan
authors.affiliation: Beijing Institute of Biotechnology, Beijing 100071, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Yang
authors.affiliation: Beijing Institute of Biotechnology, Beijing 100071, China. Electronic address: lingyanbeijing@163.com.
authors.forename: Yan
authors.initials: Y
authors.lastname: Ling
authors.affiliation: Beijing Institute of Biotechnology, Beijing 100071, China. Electronic address: chenhp0909@163.com.
authors.forename: Huipeng
authors.initials: H
authors.lastname: Chen
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D002462:Cell Membrane / Q000473:pathology; D004927:Escherichia coli Infections / Q000382:microbiology* / Q000473:pathology; D019453:Escherichia coli O157 / Q000302:isolation & purification* / Q000378:metabolism / Q000472:pathogenicity*; D029968:Escherichia coli Proteins / Q000378:metabolism; D005434:Flow Cytometry; D019073:HT29 Cells; D006367:HeLa Cells; D054884:Host-Pathogen Interactions; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008856:Microscopy, Fluorescence; D014774:Virulence; D037521:Virulence Factors / Q000378:metabolism
chemical_list: D029968:Escherichia coli Proteins; D037521:Virulence Factors; D007770:L-Lactate Dehydrogenase
keywords: Cytotoxicity; EHEC; O157:H7; Pathogenicity; Virulence