title: Uptake of clinical yeast isolates by human epithelial cell line.
abstract: OBJECTIVE
  The occurrence of yeast infections in humans has increased, with the species belonging to genus Candida still being the most common cause of infection. Nevertheless, infections caused by less common yeasts have been widely reported in recent years. The main objective of this study was to assess the potential of these less common saprophytic yeasts to invade the host cell, which is essential for causing systemic infections.
  
  
  METHODS
  Various yeast isolates were identified by DNA sequence information of PCR amplified ITS region. The purported saprophytic yeasts were characterized for internalization by mammalian cells in vitro, by staining the F-actin.
  
  
  CONCLUSIONS
  The identification of different yeast isolates from various patients revealed that 70% of the isolates belonged to the genus Candida, while remaining 30% of the isolates were yeasts not belonging to genus Candida. These non-Candida clinical isolates, either in yeast or hyphal forms, were efficiently internalized by human epithelial cells. The internalization was marked by a process of actin polymerization surrounding the invading yeast. Such uptake by epithelial cells signifies traversal of cell barrier by yeast cells during infection in vivo.
authors.affiliation: National Centre for Cell Science, Savitribai Phule Pune University Campus, Ganesh Khind, Pune 411007, India.
authors.forename: A N
authors.initials: AN
authors.lastname: Atre
authors.affiliation: National Centre for Cell Science, Savitribai Phule Pune University Campus, Ganesh Khind, Pune 411007, India.
authors.forename: A
authors.initials: A
authors.lastname: Mehta
authors.affiliation: National Centre for Cell Science, Savitribai Phule Pune University Campus, Ganesh Khind, Pune 411007, India.
authors.forename: P R
authors.initials: PR
authors.lastname: Chandorkar
authors.affiliation: National Centre for Cell Science, Savitribai Phule Pune University Campus, Ganesh Khind, Pune 411007, India. Electronic address: patole@nccs.res.in.
authors.forename: M S
authors.initials: MS
authors.lastname: Patole
authors.affiliation: M.E.S. Abasaheb Garware College, Karve Road, Pune 411004, India.
authors.forename: S S
authors.initials: SS
authors.lastname: Diwanay
authors.affiliation: Bharati Vidyapeeth's Medical College, Pune 411043, India.
authors.forename: S R
authors.initials: SR
authors.lastname: Shah
authors.affiliation: Bharati Vidyapeeth's Medical College, Pune 411043, India.
authors.forename: M S
authors.initials: MS
authors.lastname: Modak
mesh_terms: D000199:Actins / Q000378:metabolism; D002175:Candida / Q000472:pathogenicity / Q000502:physiology; D002460:Cell Line; D004705:Endocytosis / Q000502:physiology*; D004847:Epithelial Cells / Q000378:metabolism / Q000382:microbiology*; D006801:Humans; D009181:Mycoses / Q000382:microbiology*; D015003:Yeasts / Q000472:pathogenicity / Q000502:physiology*
chemical_list: D000199:Actins
keywords: Actin polymerization; Cell line; Epithelial cells; Fungal infection; Infection fongique; Internalisation; Internalization; La lignée cellulaire; Les cellules épithéliales; Levure saprophyte; Polymérisation de l’actine; Saprophytic yeast