title: Type specific Real time PCR for detection of human herpes virus 6 in schizophrenia and bipolar patients: a case control study.
abstract: BACKGROUND
  Schizophrenia (SC) and bipolar disorder (BD) are among the most devastating diseases worldwide. There are several lines of evidence suggesting that viruses may play significant roles in the etiology of these mental disorders. The aim of this study was the detection of HHV-6A/B in the peripheral blood mononuclear cells (PBMC) of SC and BD patients versus the healthy control (HC) subjects using a new method of type-specific Real time PCR analysis.
  
  
  METHODS
  A type-specific Real time PCR was performed for simultaneous detection and typing of HHV-6A/B in the PBMCs of 120 SC and BD patients and 75 HCs.
  
  
  RESULTS
  Only one case of HHV-6B out of 120 (0.8 %) SC and BD patients and two cases of HHV-6A (2.7 %) in 75 HCs were detected.
  
  
  CONCLUSIONS
  The low levels of HHV-6 detection in PBMCs, severely limited the capacity of this study to investigate the association between the presence of HHV-6 and BD or SC in this population, thus no conclusions can be drawn in this regard. Meanwhile this study introduces a Real time PCR based method for type specific detection of HHV-6A/B in clinical samples.
authors.affiliation: Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. yavarian@tums.ac.ir.
authors.forename: Jila
authors.initials: J
authors.lastname: Yavarian
authors.affiliation: Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. shatizadeh@razi.tums.ac.ir.
authors.forename: Somaye
authors.initials: S
authors.lastname: Shatizadeh Malekshahi
authors.affiliation: Department of Psychiatry, Medical School, Urmia University of Medical Sciences, Urmia, West Azarbayejan, Iran. royayavarian93@gmail.com.
authors.forename: Roya
authors.initials: R
authors.lastname: Yavarian
authors.affiliation: Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. yazdani.vrs2@gmail.com.
authors.forename: Shaghayegh
authors.initials: S
authors.lastname: Yazdani
authors.affiliation: Department of Biostatistics, School of Public Health, Iran University of Medical Sciences, Tehran, Iran. Leila_janani@yahoo.com.
authors.forename: Leila
authors.initials: L
authors.lastname: Janani
authors.affiliation: Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. nz-shafiei@tums.ac.ir.
authors.forename: Nazanin Zahra
authors.initials: NZ
authors.lastname: Shafiei Jandaghi
authors.affiliation: Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. jalalkeyani@yahoo.com.
authors.forename: Seyed Jalal
authors.initials: SJ
authors.lastname: Kiani
authors.affiliation: Department of Psychiatry, Medical School, Iran University of Medical Sciences, Tehran, Iran. dr_ahmadkhaniha@yahoo.com.
authors.forename: HamidReza
authors.initials: H
authors.lastname: Ahamadkhaniha
mesh_terms: D000328:Adult; D001714:Bipolar Disorder / Q000821:virology*; D016022:Case-Control Studies; D004279:DNA, Viral / Q000302:isolation & purification; D005260:Female; D064368:Healthy Volunteers; D015654:Herpesvirus 6, Human / Q000302:isolation & purification*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000821:virology; D008297:Male; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D019349:Roseolovirus Infections / Q000175:diagnosis* / Q000523:psychology; D012559:Schizophrenia / Q000821:virology*
chemical_list: D004279:DNA, Viral