title: Total and high molecular weight adiponectin levels in the rat model of post-myocardial infarction heart failure.
abstract: Adiponectin is a protein secreted primarily by adipose tissue. It has been suggested that adiponectin plays a protective role in the early phase following myocardial infarction. Our primary aim was to investigate the effects of post-myocardial infarction heart failure well-characterized by left ventricular hemodynamic parameters on the total and high molecular weight adiponectin concentrations in plasma, fat and cardiac tissue. Eight weeks after myocardial infarction or sham operation, total and high molecular weight adiponectin concentrations in plasma, fat, and cardiac tissues were assayed in rats. In addition, hemodynamic parameters and expression of the genes encoding atrial natriuretic peptide and brain natriuretic peptide in left ventricle were evaluated. Atrial natriuretic peptide and brain natriuretic peptide mRNA levels in left ventricle tissue were higher in rats with myocardial infarction-induced heart failure compared with the controls. Similarly, total adiponectin concentration was increased in left ventricle (but not in right ventricle) in rats with post-myocardial infarction heart failure. In contrast, adiponectin levels in plasma and cardiac adipose tissue in rats with post-myocardial infarction heart failure were lower than in sham-operated animals. Furthermore, there were no significant differences in levels of high molecular weight adiponectin in plasma, cardiac tissue or adipose tissue between these two groups. We conclude that in the rat model of post-myocardial infarction heart failure, adiponectin level is increased in left ventricle tissue. This is accompanied by decreased adiponectin levels in plasma and cardiac adipose tissue.
authors.affiliation: Department of Neuroendocrinology, Centre of Postgraduate Medical Education, Warsaw, Poland. mkalisz@cmkp.edu.pl.
authors.forename: M
authors.initials: M
authors.lastname: Kalisz
authors.affiliation: Department of Neurology, Second Faculty of Medicine, Medical University of Warsaw, Warsaw, Poland.
authors.forename: B
authors.initials: B
authors.lastname: Baranowska
authors.affiliation: Department of Neuroendocrinology, Centre of Postgraduate Medical Education, Warsaw, Poland.
authors.forename: E
authors.initials: E
authors.lastname: Wolinska-Witort
authors.affiliation: Department of Clinical Physiology, Centre of Postgraduate Medical Education, Warsaw, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Maczewski
authors.affiliation: Department of Clinical Physiology, Centre of Postgraduate Medical Education, Warsaw, Poland.
authors.forename: U
authors.initials: U
authors.lastname: Mackiewicz
authors.affiliation: Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland.
authors.forename: D
authors.initials: D
authors.lastname: Tulacz
authors.affiliation: Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Gora
authors.affiliation: Department of Neuroendocrinology, Centre of Postgraduate Medical Education, Warsaw, Poland.
authors.forename: L
authors.initials: L
authors.lastname: Martynska
authors.affiliation: Department of Neuroendocrinology, Centre of Postgraduate Medical Education, Warsaw, Poland.
authors.forename: W
authors.initials: W
authors.lastname: Bik
mesh_terms: D052242:Adiponectin / Q000378:metabolism*; D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000235:genetics; D004195:Disease Models, Animal; D006333:Heart Failure / Q000209:etiology / Q000503:physiopathology*; D006352:Heart Ventricles / Q000378:metabolism; D006439:Hemodynamics; D008297:Male; D008970:Molecular Weight; D009203:Myocardial Infarction / Q000150:complications*; D020097:Natriuretic Peptide, Brain / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
chemical_list: D052242:Adiponectin; D012333:RNA, Messenger; D020097:Natriuretic Peptide, Brain; D009320:Atrial Natriuretic Factor