title: Expression Patterns and Potential Biological Roles of Dip2a.
abstract: Disconnected (disco)-interacting protein 2 homolog A is a member of the DIP2 protein family encoded by Dip2a gene. Dip2a expression pattern has never been systematically studied. Functions of Dip2a in embryonic development and adult are not known. To investigate Dip2a gene expression and function in embryo and adult, a Dip2a-LacZ mouse model was generated by insertion of β-Gal cDNA after Dip2a promoter using CRISPR/Cas9 technology. Dip2a-LacZ mouse was designed to be a lacZ reporter mouse as well as a Dip2a knockout mouse. Heterozygous mice were used to study endogenous Dip2a expression and homozygotes to study DIP2A-associated structure and function. LacZ staining indicated that Dip2a is broadly expressed in neuronal, reproductive and vascular tissues, as well as in heart, kidney, liver and lung. Results demonstrate that Dip2a is expressed in ectoderm-derived tissues in developing embryos. Adult tissues showed rich staining in neurons, mesenchymal, endothelial, smooth muscle cells and cardiomyocytes by cell types. The expression pattern highly overlaps with FSTL1 and supports previous report that DIP2A to be potential receptor of FSTL1 and its protective roles of cardiomyocytes. Broad and intense embryonic and adult expression of Dip2a has implied their multiple structural and physiological roles.
authors.affiliation: Transgenic Research Center, School of Life Sciences, Northeast Normal University, Changchun, China.|Key Laboratory of Molecular Epigenetics of Ministry of Education, Northeast Normal University, Changchun, China.
authors.forename: Luqing
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Transgenic Research Center, School of Life Sciences, Northeast Normal University, Changchun, China.
authors.forename: Humphrey A
authors.initials: HA
authors.lastname: Mabwi
authors.affiliation: Transgenic Research Center, School of Life Sciences, Northeast Normal University, Changchun, China.
authors.forename: Norberto J
authors.initials: NJ
authors.lastname: Palange
authors.affiliation: Transgenic Research Center, School of Life Sciences, Northeast Normal University, Changchun, China.
authors.forename: Ruirui
authors.initials: R
authors.lastname: Jia
authors.affiliation: Transgenic Research Center, School of Life Sciences, Northeast Normal University, Changchun, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Ma
authors.affiliation: Transgenic Research Center, School of Life Sciences, Northeast Normal University, Changchun, China.
authors.forename: Fatoumata Binta
authors.initials: FB
authors.lastname: Bah
authors.affiliation: Transgenic Research Center, School of Life Sciences, Northeast Normal University, Changchun, China.
authors.forename: Rajiv Kumar
authors.initials: RK
authors.lastname: Sah
authors.affiliation: Transgenic Research Center, School of Life Sciences, Northeast Normal University, Changchun, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Li
authors.affiliation: Transgenic Research Center, School of Life Sciences, Northeast Normal University, Changchun, China.
authors.forename: Daji
authors.initials: D
authors.lastname: Wang
authors.affiliation: Transgenic Research Center, School of Life Sciences, Northeast Normal University, Changchun, China.
authors.forename: Fatoumata Binta Maci
authors.initials: FB
authors.lastname: Bah
authors.affiliation: Transgenic Research Center, School of Life Sciences, Northeast Normal University, Changchun, China.
authors.forename: Jacques
authors.initials: J
authors.lastname: Togo
authors.affiliation: Transgenic Research Center, School of Life Sciences, Northeast Normal University, Changchun, China.
authors.forename: Honghong
authors.initials: H
authors.lastname: Jin
authors.affiliation: Transgenic Research Center, School of Life Sciences, Northeast Normal University, Changchun, China.
authors.forename: Luying
authors.initials: L
authors.lastname: Ban
authors.affiliation: Key Laboratory of Molecular Epigenetics of Ministry of Education, Northeast Normal University, Changchun, China.
authors.forename: Xuechao
authors.initials: X
authors.lastname: Feng
authors.affiliation: Transgenic Research Center, School of Life Sciences, Northeast Normal University, Changchun, China.|Key Laboratory of Molecular Epigenetics of Ministry of Education, Northeast Normal University, Changchun, China.
authors.forename: Yaowu
authors.initials: Y
authors.lastname: Zheng
mesh_terms: D000818:Animals; D004622:Embryo, Mammalian; D005260:Female; D015870:Gene Expression; D005786:Gene Expression Regulation; D018507:Gene Expression Regulation, Developmental; D017930:Genes, Reporter; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D009687:Nuclear Proteins; D009928:Organ Specificity / Q000235:genetics; D011956:Receptors, Cell Surface / Q000235:genetics* / Q000378:metabolism*; D001616:beta-Galactosidase / Q000235:genetics
chemical_list: C554848:Dip2a protein, mouse; D009687:Nuclear Proteins; D011956:Receptors, Cell Surface; D001616:beta-Galactosidase