title: Arabidopsis non-specific phospholipase C1: characterization and its involvement in response to heat stress.
abstract: The Arabidopsis non-specific phospholipase C (NPC) protein family is encoded by the genes NPC1 - NPC6. It has been shown that NPC4 and NPC5 possess phospholipase C activity; NPC3 has lysophosphatidic acid phosphatase activity. NPC3, 4 and 5 play roles in the responses to hormones and abiotic stresses. NPC1, 2 and 6 has not been studied functionally yet. We found that Arabidopsis NPC1 expressed in Escherichia coli possesses phospholipase C activity in vitro. This protein was able to hydrolyse phosphatidylcholine to diacylglycerol. NPC1-green fluorescent protein was localized to secretory pathway compartments in Arabidopsis roots. In the knock out T-DNA insertion line NPC1 (npc1) basal thermotolerance was impaired compared with wild-type (WT); npc1 exhibited significant decreases in survival rate and chlorophyll content at the seventh day after heat stress (HS). Conversely, plants overexpressing NPC1 (NPC1-OE) were more resistant to HS compared with WT. These findings suggest that NPC1 is involved in the plant response to heat.
authors.affiliation: Institute of Experimental Botany, The Czech Academy of Sciences Prague, Czech Republic ; Department of Biochemistry and Microbiology, University of Chemistry and Technology, Prague Prague, Czech Republic.
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Krčková
authors.affiliation: Institute of Experimental Botany, The Czech Academy of Sciences Prague, Czech Republic.
authors.forename: Jitka
authors.initials: J
authors.lastname: Brouzdová
authors.affiliation: Institute of Experimental Botany, The Czech Academy of Sciences Prague, Czech Republic.
authors.forename: Michal
authors.initials: M
authors.lastname: Daněk
authors.affiliation: Institute of Experimental Botany, The Czech Academy of Sciences Prague, Czech Republic.
authors.forename: Daniela
authors.initials: D
authors.lastname: Kocourková
authors.affiliation: 1ERL Inserm U1157/UMR7203, Faculté de Medecine Pierre et Marie Curie Paris, France.
authors.forename: Dominique
authors.initials: D
authors.lastname: Rainteau
authors.affiliation: CNRS, UMR7618, Institut d'Ecologie et des Sciences de l'Environnement de Paris Créteil, France ; Université Paris Est, Institut d'Ecologie et des Sciences de l'Environnement de Paris, UPEC Créteil, France.
authors.forename: Eric
authors.initials: E
authors.lastname: Ruelland
authors.affiliation: Department of Biochemistry and Microbiology, University of Chemistry and Technology, Prague Prague, Czech Republic.
authors.forename: Olga
authors.initials: O
authors.lastname: Valentová
authors.affiliation: Institute of Experimental Botany, The Czech Academy of Sciences Prague, Czech Republic.
authors.forename: Přemysl
authors.initials: P
authors.lastname: Pejchar
authors.affiliation: Institute of Experimental Botany, The Czech Academy of Sciences Prague, Czech Republic.
authors.forename: Jan
authors.initials: J
authors.lastname: Martinec
keywords: Arabidopsis thaliana; diacylglycerol; heat stress; non-specific phospholipase C; phospholipids