title: Cone-Specific Promoters for Gene Therapy of Achromatopsia and Other Retinal Diseases.
abstract: Adeno-associated viral (AAV) vectors containing cone-specific promoters have rescued cone photoreceptor function in mouse and dog models of achromatopsia, but cone-specific promoters have not been optimized for use in primates. Using AAV vectors administered by subretinal injection, we evaluated a series of promoters based on the human L-opsin promoter, or a chimeric human cone transducin promoter, for their ability to drive gene expression of green fluorescent protein (GFP) in mice and nonhuman primates. Each of these promoters directed high-level GFP expression in mouse photoreceptors. In primates, subretinal injection of an AAV-GFP vector containing a 1.7-kb L-opsin promoter (PR1.7) achieved strong and specific GFP expression in all cone photoreceptors and was more efficient than a vector containing the 2.1-kb L-opsin promoter that was used in AAV vectors that rescued cone function in mouse and dog models of achromatopsia. A chimeric cone transducin promoter that directed strong GFP expression in mouse and dog cone photoreceptors was unable to drive GFP expression in primate cones. An AAV vector expressing a human CNGB3 gene driven by the PR1.7 promoter rescued cone function in the mouse model of achromatopsia. These results have informed the design of an AAV vector for treatment of patients with achromatopsia.
authors.affiliation: 1 Applied Genetic Technologies Corporation , Alachua, Florida.
authors.forename: Guo-Jie
authors.initials: GJ
authors.lastname: Ye
authors.affiliation: 2 Covance Laboratories Inc , Madison, Wisconsin.
authors.forename: Ewa
authors.initials: E
authors.lastname: Budzynski
authors.affiliation: 2 Covance Laboratories Inc , Madison, Wisconsin.
authors.forename: Peter
authors.initials: P
authors.lastname: Sonnentag
authors.affiliation: 3 University of Wisconsin-Madison , Madison, Wisconsin.|4 OSOD (Ocular Services On Demand), LLC , Madison, Wisconsin.
authors.forename: T Michael
authors.initials: TM
authors.lastname: Nork
authors.affiliation: 3 University of Wisconsin-Madison , Madison, Wisconsin.|4 OSOD (Ocular Services On Demand), LLC , Madison, Wisconsin.
authors.forename: Nader
authors.initials: N
authors.lastname: Sheibani
authors.affiliation: 3 University of Wisconsin-Madison , Madison, Wisconsin.
authors.forename: Zafer
authors.initials: Z
authors.lastname: Gurel
authors.affiliation: 5 University of Florida , Gainesville, Florida.
authors.forename: Sanford L
authors.initials: SL
authors.lastname: Boye
authors.affiliation: 5 University of Florida , Gainesville, Florida.
authors.forename: James J
authors.initials: JJ
authors.lastname: Peterson
authors.affiliation: 5 University of Florida , Gainesville, Florida.
authors.forename: Shannon E
authors.initials: SE
authors.lastname: Boye
authors.affiliation: 5 University of Florida , Gainesville, Florida.
authors.forename: William W
authors.initials: WW
authors.lastname: Hauswirth
authors.affiliation: 1 Applied Genetic Technologies Corporation , Alachua, Florida.
authors.forename: Jeffrey D
authors.initials: JD
authors.lastname: Chulay
mesh_terms: D000818:Animals; D003117:Color Vision Defects / Q000235:genetics* / Q000628:therapy; D000229:Dependovirus / Q000235:genetics; D004285:Dogs; D015870:Gene Expression; D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000235:genetics; D006801:Humans; D051379:Mice; D011401:Promoter Regions, Genetic; D017949:Retinal Cone Photoreceptor Cells / Q000378:metabolism* / Q000473:pathology; D012164:Retinal Diseases / Q000235:genetics* / Q000628:therapy; D017299:Rod Opsins / Q000235:genetics
chemical_list: D017299:Rod Opsins; D049452:Green Fluorescent Proteins