title: [Effect of spvB/spvC gene on Salmonella virulence and the host immune function].
abstract: OBJECTIVE
  To study the effect of spvB/spvC gene on Salmonella virulence and the Host immune.
  
  
  METHODS
  STM.211, STM.211-Delta;spvB, STM.211-Delta;spvC, STM.211-Delta;spvB.spvC and PBS were infected with 0.2 mL 10(5) CFU corresponding strain respectively by intraperitoneal. We observed the mental status, movement, diarrhea, weight, pelage changed hair of the infected mouse. Then the level of IL-10, IL-12, IFN-γ were detected by ELISA. Finally, we observe the pathological changes of liver and spleen with the general view and the microscope.
  
  
  RESULTS
  Infection symptoms of STM.211, STM.211-Delta;spvB and STM.211-Delta;spvC were significantly worse than PBS group, but there was no significant difference between STM.211-spvB.spvC group and PBS group. The secretion of IFN-γ and IL-12 of STM.211, STM.211-Delta;spvB, STM.211-Delta;spvC group were significantly lower than those in the STM.211-Delta;spvB.spvC group (P<0.05), but IL-10 secretion was significantly higher than STM.211-Delta;spvB.spvC group (P<0.05). There were no statistical significance among the STM.211, STM.211-Delta;SpvB, STM.211-Delta;spvC groups (P>0.05).
  
  
  CONCLUSIONS
  Salmonella virulence can be affected obviously by spvB combined with spvC gene, but not by spvB or spvC. spvB/spvC gene can inhibit the TH1 cytokines (IFN-γ and IL-12) secretion but promote the TH2 cytokines (IL-10) expression, leading immune response trend to TH2 shift. It shows that spvB/spvC gene can help the bacteria evade the host immune defenses, leading to aggravation of infection.
authors.affiliation: Medical Board of Nanchang University's Graduate Institute, Nanchang 330006, China.E-mail: 805953689@qq.com.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Liu
authors.forename: Qiang
authors.initials: Q
authors.lastname: Chen
authors.forename: Hong
authors.initials: H
authors.lastname: Li
authors.forename: Chunhui
authors.initials: C
authors.lastname: Zhu
authors.forename: Chunxue
authors.initials: C
authors.lastname: Wu
authors.forename: Wenxing
authors.initials: W
authors.lastname: Wang
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Yu
mesh_terms: D000818:Animals; D016207:Cytokines / Q000276:immunology; D018664:Interleukin-12; D051379:Mice; D012475:Salmonella / Q000235:genetics / Q000472:pathogenicity*; D012480:Salmonella Infections / Q000276:immunology*; D014774:Virulence; D037521:Virulence Factors / Q000235:genetics*
chemical_list: D016207:Cytokines; D037521:Virulence Factors; D018664:Interleukin-12