title: Estrogen Receptor-α Knockout Mice.
abstract: Tissue specific knockout mice are valuable tools to study gene function in vivo. The method uses the Cre/loxP system in which loxP sites are cloned into the genome surrounding one or more exons of a gene and the targeted exon(s) are deleted when the Cre enzyme is expressed. Mouse lines that are prepared for the generation of knockout ERα mice have been developed independently by many research groups and the number of available transgenic mouse lines that express Cre under tissue specific promoters is large. Here, we describe how tissue specific ERα knockout mice are generated.
authors.affiliation: Department of Biosciences and Nutrition, Karolinska Institutet, Novum, SE-141 83, Huddinge, Sweden. per.antonson@ki.se.
authors.forename: Per
authors.initials: P
authors.lastname: Antonson
authors.affiliation: Department of Biosciences and Nutrition, Karolinska Institutet, Novum, SE-141 83, Huddinge, Sweden.
authors.forename: Patricia
authors.initials: P
authors.lastname: Humire
authors.affiliation: Department of Biosciences and Nutrition, Karolinska Institutet, Novum, SE-141 83, Huddinge, Sweden.|Center for Nuclear Receptors and Cell Signaling, Department of Biology and Biochemistry, University of Houston, Houston, TX, 77204, USA.
authors.forename: Jan-Åke
authors.initials: JÅ
authors.lastname: Gustafsson
mesh_terms: D000818:Animals; D015536:Down-Regulation; D047628:Estrogen Receptor alpha / Q000172:deficiency* / Q000235:genetics; D005091:Exons; D055785:Gene Knockdown Techniques; D005838:Genotype; D019426:Integrases / Q000235:genetics; D018345:Mice, Knockout; D010641:Phenotype; D057188:Workflow
chemical_list: D047628:Estrogen Receptor alpha; C045073:Cre recombinase; D019426:Integrases
keywords: Cre enzyme; Estrogen receptor; Floxed; Gene target ing; Genotyping; Knockout mice; Tissue specific; Transgenic; loxP