title: Enhanced Tracking of Nosocomial Transmission of Endemic Sequence Type 22 Methicillin-Resistant Staphylococcus aureus Type IV Isolates among Patients and Environmental Sites by Use of Whole-Genome Sequencing.
abstract: Whole-genome sequencing (WGS) of 41 patient and environmental sequence type 22 methicillin-resistant Staphylococcus aureus staphylococcal cassette chromosome mec type IV (ST22-MRSA-IV) isolates recovered over 6 weeks in one acute hospital ward in Dublin, Ireland, where ST22-MRSA IV is endemic, revealed 228 pairwise combinations differing by <40 single nucleotide variants corresponding to potential cross-transmission events (CTEs). In contrast, 15 pairwise combinations of isolates representing five CTEs were previously identified by conventional molecular epidemiological typing. WGS enhanced ST22-MRSA-IV tracking and highlighted potential transmission of MRSA via the hospital environment.
authors.affiliation: Microbiology Research Unit, Dublin Dental University Hospital, Trinity College Dublin, University of Dublin, Dublin, Ireland.
authors.forename: Peter M
authors.initials: PM
authors.lastname: Kinnevey
authors.affiliation: Microbiology Research Unit, Dublin Dental University Hospital, Trinity College Dublin, University of Dublin, Dublin, Ireland Department of Clinical Microbiology, School of Medicine, Trinity College Dublin, St. James's Hospital, Dublin, Ireland.
authors.forename: Anna C
authors.initials: AC
authors.lastname: Shore
authors.affiliation: Department of Clinical Microbiology, School of Medicine, Trinity College Dublin, St. James's Hospital, Dublin, Ireland.
authors.forename: Micheál
authors.initials: M
authors.lastname: Mac Aogáin
authors.affiliation: Department of Microbiology, Beaumont Hospital, Dublin, Ireland.
authors.forename: Eilish
authors.initials: E
authors.lastname: Creamer
authors.affiliation: Microbiology Research Unit, Dublin Dental University Hospital, Trinity College Dublin, University of Dublin, Dublin, Ireland National MRSA Reference Laboratory, St. James's Hospital, Dublin, Ireland.
authors.forename: Gráinne I
authors.initials: GI
authors.lastname: Brennan
authors.affiliation: Department of Microbiology, Beaumont Hospital, Dublin, Ireland Department of Clinical Microbiology, Royal College of Surgeons in Ireland Education and Research Centre, Beaumont Hospital, Dublin, Ireland.
authors.forename: Hilary
authors.initials: H
authors.lastname: Humphreys
authors.affiliation: Department of Clinical Microbiology, School of Medicine, Trinity College Dublin, St. James's Hospital, Dublin, Ireland.
authors.forename: Thomas R
authors.initials: TR
authors.lastname: Rogers
authors.affiliation: Department of Clinical Microbiology, School of Medicine, Trinity College Dublin, St. James's Hospital, Dublin, Ireland National MRSA Reference Laboratory, St. James's Hospital, Dublin, Ireland.
authors.forename: Brian
authors.initials: B
authors.lastname: O'Connell
authors.affiliation: Microbiology Research Unit, Dublin Dental University Hospital, Trinity College Dublin, University of Dublin, Dublin, Ireland david.coleman@dental.tcd.ie.
authors.forename: David C
authors.identifier: http://orcid.org/0000-0003-1797-2888
authors.initials: DC
authors.lastname: Coleman
mesh_terms: D003428:Cross Infection; D004783:Environmental Microbiology; D016680:Genome, Bacterial; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D055624:Methicillin-Resistant Staphylococcus aureus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D017720:Molecular Epidemiology; D058889:Molecular Typing; D013203:Staphylococcal Infections / Q000453:epidemiology / Q000382:microbiology* / Q000635:transmission