title: NMR-Based Metabolic Snapshot from Minibronchoalveolar Lavage Fluid: An Approach To Unfold Human Respiratory Metabolomics.
abstract: The utility of mini bronchoalveolar lavage (mBAL) and its applicability in metabolomics has not been explored in the field of human respiratory disease. mBAL, "an archetype" of the local lung environment, ensures a potent technique to get the snapshot of the epithelial lining fluid afflicted to human lung disorders. Characterization of the mBAL fluid has potential to help in elucidating the composition of the alveoli and airways in the diseased state, yielding diagnostic information on clinical applicability. In this study, one of the first attempts has been made to comprehensively assign and detect metabolites in mBAL fluid, extracted from human lungs, by the composite use of 800 MHz 1D and 2D NMR, J-resolved homonuclear spectroscopy, COSY, TOCSY, and heteronuclear HSQC correlation methods. A foremost all-inclusive sketch of the 50 metabolites has been corroborated and assigned, which can be a resourceful archive to further lung-directed metabolomics, prognosis, and diagnosis. Thus, NMR-based mBALF studies, as proposed in this article, will leverage many more prospective respiratory researches for routine clinical application and prove to be a viable approach to mirror the key predisposing factors contributing to the onset of lung disease.
authors.affiliation: Centre of Biomedical Research , SGPGIMS Campus, Raebarelly Road, Lucknow 226014, India.|Faculty of Engineering and Technology, Dr. A. P. J Abdul Kalam Technical University , Lucknow 226021, India.
authors.forename: Akhila
authors.initials: A
authors.lastname: Viswan
authors.affiliation: Centre of Biomedical Research , SGPGIMS Campus, Raebarelly Road, Lucknow 226014, India.
authors.forename: Raj Kumar
authors.initials: RK
authors.lastname: Sharma
authors.affiliation: Department of Critical Care Medicine, Sanjay Gandhi Postgraduate Institute of Medical Sciences , Lucknow 226014, India.
authors.forename: Afzal
authors.initials: A
authors.lastname: Azim
authors.affiliation: Centre of Biomedical Research , SGPGIMS Campus, Raebarelly Road, Lucknow 226014, India.
authors.forename: Neeraj
authors.initials: N
authors.lastname: Sinha
mesh_terms: D015415:Biomarkers / Q000737:chemistry / Q000378:metabolism; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D055442:Metabolome; D055432:Metabolomics; D012128:Respiratory Distress Syndrome / Q000175:diagnosis / Q000378:metabolism*
chemical_list: D015415:Biomarkers
keywords: assignment; biofluids; lung disease; mini bronchoalveolar lavage fluid; two-dimensional NMR