title: Enzyme-Assisted Preparation of Furcellaran-Like κ-/β-Carrageenan.
abstract: Carrageenans are sulfated galactans that are widely used in industrial applications for their thickening and gelling properties, which vary according to the amount and distribution of ester sulfate groups along the galactan backbone. To determine and direct the sulfation of κ-carrageenan moieties, we purified an endo-κ-carrageenan sulfatase (Q15XH1 accession in UniprotKB) from Pseudoalteromonas atlantica T6c extracts. Based on sequence analyses and exploration of the genomic environment of Q15XH1, we discovered and characterized a second endo-κ-carrageenan sulfatase (Q15XG7 accession in UniprotKB). Both enzymes convert κ-carrageenan into a hybrid, furcellaran-like κ-/β-carrageenan. We compared the protein sequences of these two new κ-carrageenan sulfatases and that of a previously reported ι-carrageenan sulfatase with other predicted sulfatases in the P. atlantica genome, revealing the existence of additional new carrageenan sulfatases.
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06, UMR 8227, Integrative Biology of Marine Models, Station Biologique de Roscoff, 29680, Roscoff, France.|CNRS, UMR 8227, Integrative Biology of Marine Models, Station Biologique de Roscoff, 29680, Roscoff, France.
authors.forename: Aurélie
authors.initials: A
authors.lastname: Préchoux
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06, UMR 8227, Integrative Biology of Marine Models, Station Biologique de Roscoff, 29680, Roscoff, France.|CNRS, UMR 8227, Integrative Biology of Marine Models, Station Biologique de Roscoff, 29680, Roscoff, France.
authors.forename: Sabine
authors.initials: S
authors.lastname: Genicot
authors.affiliation: INRA, Biopolymers Interactions Assemblies, 44316, Nantes, France.
authors.forename: Hélène
authors.initials: H
authors.lastname: Rogniaux
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06, UMR 8227, Integrative Biology of Marine Models, Station Biologique de Roscoff, 29680, Roscoff, France. helbert@cermav.cnrs.fr.|CNRS, UMR 8227, Integrative Biology of Marine Models, Station Biologique de Roscoff, 29680, Roscoff, France. helbert@cermav.cnrs.fr.|Centre de Recherches sur les Macromolécules Végétales (CERMAV, UPR-CNRS 5301), Affiliated with the Université Joseph Fourier (UJF), BP53, 38041, Grenoble Cedex 9, France. helbert@cermav.cnrs.fr.|Institut de Chimie Moléculaire de Grenoble (ICMG, FR-CNRS 2607), Grenoble Cedex 9, France. helbert@cermav.cnrs.fr.
authors.forename: William
authors.initials: W
authors.lastname: Helbert
mesh_terms: D000464:Alginates / Q000138:chemical synthesis*; D000595:Amino Acid Sequence; D002351:Carrageenan / Q000138:chemical synthesis*; D004789:Enzyme Activation; D008969:Molecular Sequence Data; D053149:Plant Gums / Q000138:chemical synthesis*; D031161:Pseudoalteromonas / Q000145:classification / Q000201:enzymology*; D013045:Species Specificity; D013329:Structure-Activity Relationship; D013429:Sulfatases / Q000737:chemistry*
chemical_list: D000464:Alginates; D053149:Plant Gums; D002351:Carrageenan; C012985:furcellaran; D013429:Sulfatases
keywords: Biotransformation; Carrageenan; Pseudoalteromonas atlantica; Sulfatase