title: Use of Buffy Coat miRNA Profiling for Breast Cancer Prediction in Healthy Women.
abstract: MicroRNAs are key regulators of different biological processes and their deregulation is associated with the occurrence of many diseases among which cancer. Due to the higher stability of microRNAs and to the easiness in their detection both in organs than in biological fluids, many studies are turned toward potential use of this small molecules as biomarkers for the prediction and diagnosis of different types of cancer. Here we describe the experiment protocol that we have used for microRNA profiling analysis in buffy coat samples of women who developed breast cancer versus women who remained healthy during a 20 year follow-up period, with the aim to identify predictive microRNAs of breast cancer occurrence.
authors.affiliation: Translational Oncogenomics Unit, Regina Elena Italian National Cancer Institute, Rome, Italy.
authors.forename: Sara
authors.initials: S
authors.lastname: Donzelli
authors.affiliation: Laboratory of Translational Oncogenomics, Molecular Medicine Department, Regina Elena National Cancer Institute, Rome, Italy.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Blandino
authors.affiliation: Department of Oncology, Faculty of Health Science, McMaster University, 1280 Main Street West, Hamilton, ON, Canada. muti@mcmaster.ca.
authors.forename: Paola
authors.initials: P
authors.lastname: Muti
mesh_terms: D058621:Blood Buffy Coat / Q000378:metabolism*; D001943:Breast Neoplasms / Q000097:blood*; D019295:Computational Biology / Q000379:methods*; D005260:Female; D006262:Health; D006801:Humans; D007962:Leukocytes / Q000166:cytology; D035683:MicroRNAs / Q000097:blood* / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis
chemical_list: D035683:MicroRNAs
keywords: Breast cancer; Buffy coat; RNA extraction; microRNA; microRNA profiling