title: Expression of B7-H3 in cancer tissue during osteosarcoma progression in nude mice.
abstract: Immune cells might participate in the ontogenesis of osteosarcoma. B7-H3 is a new discovered T cell co-stimulatory molecule that was found to be overexpressed in malignant tumors. We aimed to investigate the dynamic expression level of B7-H3 in nude mice with osteosarcoma. A nude mouse osteosarcoma model was successfully established. B7-H3 expression and distribution changes in the early, middle, and late phases of osteosarcoma formation after tumor implantation were observed. Reverse transcription-polymerase chain reaction and western blot analyses were applied to measure the B7-H3 mRNA and protein dynamic changes. Confocal microscopy and immunohistochemistry were used to determine B7-H3 localization and CD3+ T cell expression, respectively, in osteosarcoma tissue. B7-H3 mRNA and protein levels fluctuated during the process of osteosarcoma formation in the nude mouse model. Expression levels were lower in the early and middle phases, while B7-H3 mRNA and protein were overexpressed in the late stage. Accordingly, CD3+ T cell numbers in the early, middle, and late phases in osteosarcoma tissue were 93 ± 13, 92 ± 12, and 46 ± 15, respectively; they can be seen to have decreased significantly in the late stage (P < 0.05). Overall, our results indicated that the B7-H3 expression level is correlated with tumor volume and severity; therefore, it might serve as a tumor biomarker for osteosarcoma.
authors.affiliation: Department of Spine Surgery, The People's Hospital of Shouguang, Shouguang, Shandong Province, China.
authors.forename: S J
authors.initials: SJ
authors.lastname: Yin
authors.affiliation: Department of Spine Surgery, The People's Hospital of Shouguang, Shouguang, Shandong Province, China.
authors.forename: W J
authors.initials: WJ
authors.lastname: Wang
authors.affiliation: Department of Spine Surgery, The People's Hospital of Shouguang, Shouguang, Shandong Province, China.
authors.forename: J Y
authors.initials: JY
authors.lastname: Zhang
mesh_terms: D000818:Animals; D060887:B7 Antigens / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D014408:Biomarkers, Tumor / Q000096:biosynthesis / Q000235:genetics; D001859:Bone Neoplasms / Q000235:genetics / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D018450:Disease Progression; D015972:Gene Expression Regulation, Neoplastic; D051379:Mice; D008819:Mice, Nude; D012516:Osteosarcoma / Q000235:genetics / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D060887:B7 Antigens; D014408:Biomarkers, Tumor; C486038:Cd276 protein, mouse; D012333:RNA, Messenger