title: [HRM identification of Chinese medicinal materials Mutong].
abstract: High resolution melting (HRM), an important technology for genotyping and mutation scanning, has broad prospects in the authentification of traditional Chinese medicine. This paper selected universal trnH-psbA primers and used HRM to establish a new methods for identification of Akebia herbs. PCR was conduct at the annealing temperature of 58 degrees C and 35 cycles. The range of the DNA template concentration, the primer concentration and the Mg2+ ion concentration were further analyzed. The results showed the Tm values of Caulis Akebiae was (81.84 ± 0.16), (85.28 ± 0.16) degrees C and Caulis Clematidis Armandii was (83.22 ± 0.19) degrees C and Caulis Aristolochiae manshuriensis was (81.67 ± 0.14) degrees C, (84.24 ± 0.10) degrees C with 5-125 mg - L-' DNA template, 0.4 μmol x L(-1) primer, 2.0 mmol x L(-1) Mg2+. This method can achieve the authentification of Akebia herbs and is simple, fast, high-throughput, visual.
authors.forename: Jun
authors.initials: J
authors.lastname: Hu
authors.forename: Zhi-lai
authors.initials: ZL
authors.lastname: Zhan
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yuan
authors.forename: Lu-qi
authors.initials: LQ
authors.lastname: Huang
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
mesh_terms: D002623:Chemistry Techniques, Analytical / Q000379:methods*; D018744:DNA, Plant / Q000737:chemistry* / Q000235:genetics; D005838:Genotype; D019684:Magnoliopsida / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D044366:Transition Temperature
chemical_list: D018744:DNA, Plant