title: Properties of Polyhydroxyalkanoate Granules and Bioemulsifiers from Pseudomonas sp. and Burkholderia sp. Isolates Growing on Glucose.
abstract: A Burkholderia and Pseudomonas species designated as AB4 and AS1, respectively, were isolated from soil containing decomposing straw or sugar cane bagasse collected from Brazil. This study sought to evaluate the capacities of culture media, cell-free medium, and crude lysate preparations (containing PHB inclusion bodies) from bacterial cell cultures to stabilize emulsions with several hydrophobic compounds. Four conditions showed good production of bioemulsifiers (E24 ≥ 50 %), headed by substantially cell-free media from bacterial cell cultures in which bacterial isolates from Burkholderia sp. strain AB4 and Pseudomonas sp. strain AS1 were grown. Our results revealed that the both isolates (AB4 and AS1 strains) exhibited high emulsification indices (indicating usefulness in bioremediation) and good stabilities.
authors.affiliation: Departamento de Tecnologia, Faculdade de Ciências Agrárias e Veterinárias, UNESP-Univ Estadual Paulista, Rod. Prof. Paulo Donato Castellane km 5, CEP 14884-900, Jaboticabal, SP, Brazil.
authors.forename: Laís Postai
authors.initials: LP
authors.lastname: Sacco
authors.affiliation: Departamento de Tecnologia, Faculdade de Ciências Agrárias e Veterinárias, UNESP-Univ Estadual Paulista, Rod. Prof. Paulo Donato Castellane km 5, CEP 14884-900, Jaboticabal, SP, Brazil. teluque@yahoo.com.br.
authors.forename: Tereza Cristina Luque
authors.initials: TC
authors.lastname: Castellane
authors.affiliation: Departamento de Tecnologia, Faculdade de Ciências Agrárias e Veterinárias, UNESP-Univ Estadual Paulista, Rod. Prof. Paulo Donato Castellane km 5, CEP 14884-900, Jaboticabal, SP, Brazil.
authors.forename: Erica Mendes
authors.initials: EM
authors.lastname: Lopes
authors.affiliation: Departamento de Tecnologia, Faculdade de Ciências Agrárias e Veterinárias, UNESP-Univ Estadual Paulista, Rod. Prof. Paulo Donato Castellane km 5, CEP 14884-900, Jaboticabal, SP, Brazil.
authors.forename: Eliana Gertrudes
authors.initials: EG
authors.lastname: de Macedo Lemos
authors.affiliation: Departamento de Tecnologia, Faculdade de Ciências Agrárias e Veterinárias, UNESP-Univ Estadual Paulista, Rod. Prof. Paulo Donato Castellane km 5, CEP 14884-900, Jaboticabal, SP, Brazil. lmcalves@fcav.unesp.br.
authors.forename: Lúcia Maria Carareto
authors.initials: LM
authors.lastname: Alves
mesh_terms: D018533:Biomass; D019117:Burkholderia / Q000254:growth & development / Q000378:metabolism*; D004655:Emulsions; D005947:Glucose / Q000378:metabolism*; D054813:Polyhydroxyalkanoates / Q000378:metabolism*; D011549:Pseudomonas / Q000254:growth & development / Q000378:metabolism*
chemical_list: D004655:Emulsions; D054813:Polyhydroxyalkanoates; D005947:Glucose
keywords: Bioemulsifier; Biopolymers; Burkholderia sp.; Emulsifying activity; Polyhydroxybutyrate; Pseudomonas sp.