title: Multiresidue analysis of multiclass pesticides and polyaromatic hydrocarbons in fatty fish by gas chromatography tandem mass spectrometry and evaluation of matrix effect.
abstract: This paper reports a selective and sensitive method for multiresidue determination of 119 chemical residues including pesticides and polyaromatic hydrocarbons (PAH) in high fatty fish matrix. The novel sample preparation method involved extraction of the target analytes from homogenized fish meat (5 g) in acetonitrile (15 mL, 1% acetic acid) after three-phase partitioning with hexane (2 mL) and the remaining aqueous layer. An aliquot (1.5 mL) of the acetonitrile layer was aspirated and subjected to two-stage dispersive solid phase extraction (dSPE) cleanup and the residues were finally estimated by gas chromatography mass spectrometry with selected reaction monitoring (GC-MS/MS). The co-eluted matrix components were identified on the basis of their accurate mass by GC with quadrupole time of flight MS. Addition of hexane during extraction and optimized dSPE cleanup significantly minimized the matrix effects. Recoveries at 10, 25 and 50 μg/kg were within 60-120% with associated precision, RSD<11%.
authors.affiliation: ICAR-Central Institute of Fisheries Technology, CIFT Junction, Matsyapuri P.O., Willingdon Island, Cochin 682029, India.
authors.forename: Niladri S
authors.initials: NS
authors.lastname: Chatterjee
authors.affiliation: National Referral Laboratory, ICAR-National Research Centre for Grapes, P.O. No. 3, Manjri Farm, Pune 412 307, India.
authors.forename: Sagar
authors.initials: S
authors.lastname: Utture
authors.affiliation: National Referral Laboratory, ICAR-National Research Centre for Grapes, P.O. No. 3, Manjri Farm, Pune 412 307, India. Electronic address: kbgrape@yahoo.com.
authors.forename: Kaushik
authors.initials: K
authors.lastname: Banerjee
authors.affiliation: National Referral Laboratory, ICAR-National Research Centre for Grapes, P.O. No. 3, Manjri Farm, Pune 412 307, India.
authors.forename: T P
authors.initials: TP
authors.lastname: Ahammed Shabeer
authors.affiliation: National Referral Laboratory, ICAR-National Research Centre for Grapes, P.O. No. 3, Manjri Farm, Pune 412 307, India.
authors.forename: Narayan
authors.initials: N
authors.lastname: Kamble
authors.affiliation: ICAR-Central Institute of Fisheries Technology, CIFT Junction, Matsyapuri P.O., Willingdon Island, Cochin 682029, India.
authors.forename: Suseela
authors.initials: S
authors.lastname: Mathew
authors.affiliation: ICAR-Central Institute of Fisheries Technology, CIFT Junction, Matsyapuri P.O., Willingdon Island, Cochin 682029, India.
authors.forename: K
authors.initials: K
authors.lastname: Ashok Kumar
mesh_terms: D000818:Animals; D005399:Fishes / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006838:Hydrocarbons / Q000032:analysis*; D010573:Pesticide Residues / Q000032:analysis / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000379:methods
chemical_list: D006838:Hydrocarbons; D010573:Pesticide Residues
keywords: Fatty fish; Gas chromatography mass spectrometry; Matrix effect; Pesticide residues; Polyaromatic hydrocarbons; Sample preparation