title: Therapy of Chronic Cardiosclerosis in WAG Rats Using Cultures of Cardiovascular Cells Enriched with Cardiac Stem Cell.
abstract: We developed a protocol for preparing cardiac cell culture from rat heart enriched with regional stem cells based on clonogenic properties and proliferation in culture in a medium with low serum content. Experiments on WAG rats with experimental ischemic myocardial damage showed that implantation of autologous regional stem cells into the left ventricle reduced the volume of cicatricial tissue, promoted angiogenesis in the damaged zone, and prevented the risk of heart failure development.
authors.affiliation: Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Sciences, Moscow, Russia.|Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Moscow, Russia.|E. N. Meshalkin Research Institute of Circulatory Pathology, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: E V
authors.initials: EV
authors.lastname: Chepeleva
authors.affiliation: Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Sciences, Moscow, Russia.|Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Moscow, Russia.|E. N. Meshalkin Research Institute of Circulatory Pathology, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: S V
authors.initials: SV
authors.lastname: Pavlova
authors.affiliation: Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Sciences, Moscow, Russia.|Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Moscow, Russia.|E. N. Meshalkin Research Institute of Circulatory Pathology, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: A A
authors.initials: AA
authors.lastname: Malakhova
authors.affiliation: E. N. Meshalkin Research Institute of Circulatory Pathology, Ministry of Health of the Russian Federation, Moscow, Russia.|Novosibirsk National Research State University, Novosibirsk, Russia.
authors.forename: E A
authors.initials: EA
authors.lastname: Milevskaya
authors.affiliation: E. N. Meshalkin Research Institute of Circulatory Pathology, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: Ya L
authors.initials: YL
authors.lastname: Rusakova
authors.affiliation: E. N. Meshalkin Research Institute of Circulatory Pathology, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: N A
authors.initials: NA
authors.lastname: Podkhvatilina
authors.affiliation: E. N. Meshalkin Research Institute of Circulatory Pathology, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: D S
authors.initials: DS
authors.lastname: Sergeevichev
authors.affiliation: E. N. Meshalkin Research Institute of Circulatory Pathology, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: E A
authors.initials: EA
authors.lastname: Pokushalov
authors.affiliation: E. N. Meshalkin Research Institute of Circulatory Pathology, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: A M
authors.initials: AM
authors.lastname: Karaskov
authors.affiliation: V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: G T
authors.initials: GT
authors.lastname: Sukhikh
authors.affiliation: Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Sciences, Moscow, Russia. zakian@bionet.nsc.ru.|Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Moscow, Russia. zakian@bionet.nsc.ru.|E. N. Meshalkin Research Institute of Circulatory Pathology, Ministry of Health of the Russian Federation, Moscow, Russia. zakian@bionet.nsc.ru.|Novosibirsk National Research State University, Novosibirsk, Russia. zakian@bionet.nsc.ru.
authors.forename: S M
authors.initials: SM
authors.lastname: Zakiyan
mesh_terms: D000818:Animals; D000943:Antigens, Differentiation / Q000032:analysis / Q000235:genetics; D002454:Cell Differentiation; D002455:Cell Division; D002478:Cells, Cultured; D003470:Culture Media / Q000494:pharmacology; D004195:Disease Models, Animal; D005312:Fetal Blood; D017930:Genes, Reporter; D006325:Heart Atria / Q000166:cytology; D006333:Heart Failure / Q000517:prevention & control; D006352:Heart Ventricles; D037102:Lectins / Q000378:metabolism; D008077:Lipoproteins, LDL / Q000378:metabolism; D008297:Male; D039902:Multipotent Stem Cells / Q000166:cytology / Q000187:drug effects; D017202:Myocardial Ischemia / Q000601:surgery*; D009206:Myocardium / Q000473:pathology; D032383:Myocytes, Cardiac / Q000166:cytology; D032389:Myocytes, Smooth Muscle / Q000166:cytology; D058628:Myofibroblasts / Q000166:cytology; D018919:Neovascularization, Physiologic; D020286:Pericytes / Q000166:cytology; D051381:Rats; D011919:Rats, Inbred Strains; D012598:Sclerosis; D033581:Stem Cell Transplantation
chemical_list: D000943:Antigens, Differentiation; D003470:Culture Media; D037102:Lectins; D008077:Lipoproteins, LDL; C032629:acetyl-LDL
keywords: cardiac stem cells; cell therapy; myocardial infarction