title: Profiling of the Early Nitrogen Stress Response in the Diatom Phaeodactylum tricornutum Reveals a Novel Family of RING-Domain Transcription Factors.
abstract: Diatoms often inhabit highly variable habitats where they are confronted with a wide variety of stresses, frequently including starvation of nutrients such as nitrogen. In this study, the transcriptome of the model diatom Phaeodactylum tricornutum was profiled during the onset of nitrogen starvation by RNA sequencing, and overrepresented motifs were determined in promoters of genes that were early and strongly up-regulated during the nitrogen stress response. One of these motifs could be bound by a nitrogen starvation-inducible RING-domain protein termed RING-GAF-Gln-containing protein (RGQ1), which was shown to act as a transcription factor and belongs to a previously uncharacterized family that is conserved in heterokont algae.
authors.affiliation: Department of Plant Systems Biology (M.M., M.F., A.G.) and Inflammation Research Center (S.B.), VIB, B-9052 Gent, Belgium; Department of Plant Biotechnology and Bioinformatics (M.M., M.F., A.G.) and Department of Biomedical Molecular Biology (S.B.), Ghent University, B-9052 Gent, Belgium; and Department of Biology, Laboratory of Protistology and Aquatic Ecology, Ghent University, B-9000 Gent, Belgium (M.M., M.F., W.V.).
authors.forename: Michiel
authors.initials: M
authors.lastname: Matthijs
authors.affiliation: Department of Plant Systems Biology (M.M., M.F., A.G.) and Inflammation Research Center (S.B.), VIB, B-9052 Gent, Belgium; Department of Plant Biotechnology and Bioinformatics (M.M., M.F., A.G.) and Department of Biomedical Molecular Biology (S.B.), Ghent University, B-9052 Gent, Belgium; and Department of Biology, Laboratory of Protistology and Aquatic Ecology, Ghent University, B-9000 Gent, Belgium (M.M., M.F., W.V.).
authors.forename: Michele
authors.initials: M
authors.lastname: Fabris
authors.affiliation: Department of Plant Systems Biology (M.M., M.F., A.G.) and Inflammation Research Center (S.B.), VIB, B-9052 Gent, Belgium; Department of Plant Biotechnology and Bioinformatics (M.M., M.F., A.G.) and Department of Biomedical Molecular Biology (S.B.), Ghent University, B-9052 Gent, Belgium; and Department of Biology, Laboratory of Protistology and Aquatic Ecology, Ghent University, B-9000 Gent, Belgium (M.M., M.F., W.V.).
authors.forename: Stefan
authors.initials: S
authors.lastname: Broos
authors.affiliation: Department of Plant Systems Biology (M.M., M.F., A.G.) and Inflammation Research Center (S.B.), VIB, B-9052 Gent, Belgium; Department of Plant Biotechnology and Bioinformatics (M.M., M.F., A.G.) and Department of Biomedical Molecular Biology (S.B.), Ghent University, B-9052 Gent, Belgium; and Department of Biology, Laboratory of Protistology and Aquatic Ecology, Ghent University, B-9000 Gent, Belgium (M.M., M.F., W.V.).
authors.forename: Wim
authors.initials: W
authors.lastname: Vyverman
authors.affiliation: Department of Plant Systems Biology (M.M., M.F., A.G.) and Inflammation Research Center (S.B.), VIB, B-9052 Gent, Belgium; Department of Plant Biotechnology and Bioinformatics (M.M., M.F., A.G.) and Department of Biomedical Molecular Biology (S.B.), Ghent University, B-9052 Gent, Belgium; and Department of Biology, Laboratory of Protistology and Aquatic Ecology, Ghent University, B-9000 Gent, Belgium (M.M., M.F., W.V.) algoo@psb.vib-ugent.be.
authors.forename: Alain
authors.identifier: http://orcid.org/0000-0002-1599-551X
authors.initials: A
authors.lastname: Goossens
mesh_terms: D017377:Diatoms / Q000502:physiology*; D020869:Gene Expression Profiling; D005810:Multigene Family; D009584:Nitrogen / Q000378:metabolism; D011401:Promoter Regions, Genetic; D017434:Protein Structure, Tertiary; D013312:Stress, Physiological; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism*; D059467:Transcriptome
chemical_list: D014157:Transcription Factors; D009584:Nitrogen