title: Oral brush biopsy and melanoma-associated antigens A (MAGE-A) staining in clinically suspicious lesions.
abstract: OBJECTIVE
  In oral cancer and in other tumor entities, melanoma-associated antigens are present. These antigens contribute to tumor progression and poor prognosis, and reduce the cytotoxicity of antineoplastic drugs. The aim of this study was to investigate the diagnostic potential of these antigens in combination with oral brush biopsies.
  
  
  METHODS
  We analyzed 72 oral brush biopsy specimens for melanoma-associated antigens A (MAGE-A) expression by immunocytologic staining with the MAGE-A 57B antibody. A total of 24 healthy specimens, 15 lichen ruber cases, 18 leukoplakia cases, and 15 invasive carcinomas were studied. Incisional biopsy served as the gold standard.
  
  
  RESULTS
  In total, 66 of 72 specimens (91.6%) could be assessed. Twelve of 15 (80%) carcinomas stained positive for MAGE-A. MAGE-A staining was detected in four of 51 nonmalignant specimens, resulting in a false-positive rate of 7.8%. However, MAGE-A positive staining was significantly correlated with oral squamous cell carcinoma (p < 0.0005). Sensitivity and specificity for MAGE-A staining and carcinoma were 80% and 92.2%, respectively. The diagnostic accuracy was 89.4%.
  
  
  CONCLUSIONS
  Our results indicate that oral brush biopsy with MAGE-A staining serves as an additional tool for use in oral cancer diagnosis. These findings might help to facilitate an easier and more representative surveillance of the mucosa, particularly for large areas of altered mucosa.
authors.affiliation: Department of Oral and Maxillofacial Plastic Surgery (Head: A. C. Kübler), University Hospital Würzburg, Pleicherwall 2, 97070 Würzburg, Germany. Electronic address: hartmann_s2@ukw.de.
authors.forename: Stefan
authors.initials: S
authors.lastname: Hartmann
authors.affiliation: Department of Oral and Maxillofacial Plastic Surgery (Head: A. C. Kübler), University Hospital Würzburg, Pleicherwall 2, 97070 Würzburg, Germany.
authors.forename: Romy U N
authors.initials: RU
authors.lastname: Kipke
authors.affiliation: Institute of Pathology (Head: A. Rosenwald), University Würzburg, Josef-Schneider-Straße 2, 97080 Würzburg, Germany; Comprehensive Cancer Center Mainfranken (Head: R. Bargou), Josef-Schneider-Straße 6, 97080 Würzburg, Germany.
authors.forename: Stephan
authors.initials: S
authors.lastname: Rauthe
authors.affiliation: Institute of Pathology (Head: A. Rosenwald), University Würzburg, Josef-Schneider-Straße 2, 97080 Würzburg, Germany; Comprehensive Cancer Center Mainfranken (Head: R. Bargou), Josef-Schneider-Straße 6, 97080 Würzburg, Germany.
authors.forename: Grit
authors.initials: G
authors.lastname: Mutzbauer
authors.affiliation: Department of Oral and Maxillofacial Plastic Surgery (Head: A. C. Kübler), University Hospital Würzburg, Pleicherwall 2, 97070 Würzburg, Germany; Comprehensive Cancer Center Mainfranken (Head: R. Bargou), Josef-Schneider-Straße 6, 97080 Würzburg, Germany.
authors.forename: Roman C
authors.initials: RC
authors.lastname: Brands
authors.affiliation: Center for Oral Pathology, Friedrich-Ebert-Str. 33-34, 14469 Potsdam, Germany.
authors.forename: Harald
authors.initials: H
authors.lastname: Ebhardt
authors.affiliation: Department of Oral and Maxillofacial Plastic Surgery (Head: A. C. Kübler), University Hospital Würzburg, Pleicherwall 2, 97070 Würzburg, Germany.
authors.forename: Alexander C
authors.initials: AC
authors.lastname: Kübler
authors.affiliation: Department of Oral and Maxillofacial Plastic Surgery (Head: A. C. Kübler), University Hospital Würzburg, Pleicherwall 2, 97070 Würzburg, Germany.
authors.forename: Urs D A
authors.initials: UD
authors.lastname: Müller-Richter
mesh_terms: D001706:Biopsy; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis / Q000276:immunology*; D006801:Humans; D058950:Melanoma-Specific Antigens / Q000032:analysis / Q000276:immunology*; D009062:Mouth Neoplasms / Q000175:diagnosis / Q000276:immunology*; D009363:Neoplasm Proteins; D013194:Staining and Labeling
chemical_list: D058950:Melanoma-Specific Antigens; D009363:Neoplasm Proteins
keywords: Brush biopsy; HNSCC; MAGE-A; Melanoma-associated antigens; Oral cancer