title: The Level of ALR is Regulated by the Quantity of Mitochondrial DNA.
abstract: Augmenter of liver regeneration (ALR) contributes to mitochondrial biogenesis, maintenance and to the physiological operation of mitochondria. The depletion of ALR has been widely studied and had serious consequences on the mitochondrial functions. However the inverse direction, the effect of the depletion of mitochondrial electron transfer chain and mtDNA on ALR expression has not been investigated yet. Thus mtDNA depleted, ρ(0) cell line was prepared to investigate the role of mitochondrial electron transfer chain and mtDNA on ALR expression. The depletion of mtDNA has not caused any difference at mRNA level, but at protein level the expression of ALR has been markedly increased. The regulatory role of ATP and ROS levels could be ruled out because the treatment of the parental cell line with different respiratory inhibitors and uncoupling agent could not provoke any changes in the protein level of ALR. The effect of mtDNA depletion on the protein level of ALR has been proved not to be liver specific, since the phenomenon could be observed in the case of two other, non-hepatic cell lines. It seems the level of mtDNA and/or its products may have regulatory role on the protein level of ALR. The up-regulation of ALR can be a part of the adaptive response in ρ(0) cells that preserves the structural integrity and the transmembrane potential despite the absence of protein components encoded by the mtDNA.
authors.affiliation: Department of Applied Biotechnology and Food Science, Laboratory of Biochemistry and Molecular Biology, Budapest University of Technology and Economics, Szent Gellért tér 4, Budapest, 1111, Hungary.
authors.forename: Tibor
authors.initials: T
authors.lastname: Balogh
authors.affiliation: Department of Applied Biotechnology and Food Science, Laboratory of Biochemistry and Molecular Biology, Budapest University of Technology and Economics, Szent Gellért tér 4, Budapest, 1111, Hungary.
authors.forename: Tamás
authors.initials: T
authors.lastname: Lőrincz
authors.affiliation: Pathobiochemistry Research Group of Hungarian Academy of Sciences, Semmelweis University, P.O. Box 260, Budapest, 1444, Hungary.
authors.forename: Ibolya
authors.initials: I
authors.lastname: Stiller
authors.affiliation: Department of Medical Chemistry, Molecular Biology and Pathobiochemistry Pathobiochemistry, Semmelweis University, POB 260, Budapest, 1444, Hungary.
authors.forename: József
authors.initials: J
authors.lastname: Mandl
authors.affiliation: Department of Medical Chemistry, Molecular Biology and Pathobiochemistry Pathobiochemistry, Semmelweis University, POB 260, Budapest, 1444, Hungary.
authors.forename: Gábor
authors.initials: G
authors.lastname: Bánhegyi
authors.affiliation: Department of Applied Biotechnology and Food Science, Laboratory of Biochemistry and Molecular Biology, Budapest University of Technology and Economics, Szent Gellért tér 4, Budapest, 1111, Hungary. szarka@mail.bme.hu.|Pathobiochemistry Research Group of Hungarian Academy of Sciences, Semmelweis University, P.O. Box 260, Budapest, 1444, Hungary. szarka@mail.bme.hu.
authors.forename: András
authors.initials: A
authors.lastname: Szarka
mesh_terms: D015153:Blotting, Western; D049109:Cell Proliferation; D004272:DNA, Mitochondrial / Q000235:genetics*; D004579:Electron Transport; D006801:Humans; D008115:Liver Regeneration / Q000502:physiology*; D008928:Mitochondria / Q000378:metabolism* / Q000473:pathology; D009369:Neoplasms / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
chemical_list: D004272:DNA, Mitochondrial; D012333:RNA, Messenger
keywords: Augmenter of liver regeneration; Mitochondrial DNA; Regulation; ρ0 cells