title: Effects of Soy Phytoestrogens and New Zealand Functional Foods on Bone Health.
abstract: New Zealand is a rich source of food components that may have bioactivity on bone. Docosahexaenoic acid (DHA) from fish oil has been shown to maintain bone in ovariectomised (OVX) rats. Kiwifruit, a source of fibre and carotenoids, may also affect bone via a prebiotic as well as direct cell-based mechanisms. We aimed to 1) ascertain the effects of DHA on two cell models, including interactions with soy isoflavones; 2) and investigate the specific effects of carotenoids from kiwifruit as well as whole kiwifruit in cell-based and rodent models as well as in a human study. RAW 264.7 mouse monocytes or mouse bone marrow was used to generate osteoclasts (OC). Cells were exposed to the agents between 5 and 21 d and formation and activity of OC measured, including molecular markers. DHA inhibited OC formation in both cell models, including expression of cathepsin K, NFATc1 as well as actin ring formation. Combination with isoflavones enhanced these effects. In OVX rats and mice fed with kiwifruit for 8 wk, green kiwifruit reduced the rate of bone loss after OVX, and in mice it reduced C-telopeptide of Type 1 collagen (CTX) levels and RANKL expression while in menopausal women, green kiwifruit affected blood lipids and bone markers positively.
authors.affiliation: School of Food and Nutrition, Massey University, New Zealand; Department of Human Nutrition, Faculty of Health Sciences, University of Pretoria.
authors.forename: M C
authors.initials: MC
authors.lastname: Kruger
authors.forename: Y
authors.initials: Y
authors.lastname: Tousen
authors.forename: S
authors.initials: S
authors.lastname: Katsumata
authors.forename: M
authors.initials: M
authors.lastname: Tadaishi
authors.forename: A E
authors.initials: AE
authors.lastname: Kasonga
authors.forename: V
authors.initials: V
authors.lastname: Deepak
authors.forename: M
authors.initials: M
authors.lastname: Coetzee
authors.forename: Y
authors.initials: Y
authors.lastname: Ishimi
mesh_terms: D029042:Actinidia; D000818:Animals; D015519:Bone Density / Q000187:drug effects; D001862:Bone Resorption / Q000378:metabolism / Q000517:prevention & control; D001842:Bone and Bones / Q000187:drug effects* / Q000378:metabolism; D002338:Carotenoids / Q000494:pharmacology / Q000627:therapeutic use; D004032:Diet; D004281:Docosahexaenoic Acids / Q000494:pharmacology / Q000627:therapeutic use*; D005260:Female; D005638:Fruit; D055951:Functional Food; D006801:Humans; D007529:Isoflavones / Q000494:pharmacology / Q000627:therapeutic use; D051379:Mice; D009520:New Zealand; D010024:Osteoporosis / Q000378:metabolism / Q000517:prevention & control*; D048789:Phytoestrogens / Q000494:pharmacology / Q000627:therapeutic use*; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use; D000067996:RAW 264.7 Cells; D051381:Rats; D013025:Glycine max / Q000737:chemistry*
chemical_list: D007529:Isoflavones; D048789:Phytoestrogens; D010936:Plant Extracts; D004281:Docosahexaenoic Acids; D002338:Carotenoids