title: Cnuibacter physcomitrellaegen. nov., sp. nov., a novel member of the familyMicrobacteriaceaeisolated from the moss ofPhyscomitrella patens.
abstract: A Gram-stain-positive, strictly aerobic and rod-shaped bacterium, designated strain XAT, was isolated from the moss,Physcomitrella patens, and characterized by using a polyphasic taxonomic approach. The novel strain was non-spore-forming, non-motile, catalase-positive and oxidase-negative. Its optimal temperature for growth occurred at 28-30 °C and the optimum pH for growth was 7.0-7.5. The major fatty acids comprised anteiso-C15 : 0, iso-C16 : 0, ω-cyclohexyl-C17 : 0and anteiso-C17 : 0. The polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, seven unidentified glycolipids, one unidentified phospholipid and one unidentified lipid. The major menaquinone was MK-11, followed by MK-10 and MK-12. The peptidoglycan type was B2γ and contained the amino acids 2,4-diaminobutyric acid, alanine, glycine, 3-hydroxy-glutamic acid and small amounts of glutamic acid. The genomic DNA G+C content of strain XATwas 68.2 mol%. Strain XATexhibited highest 16S rRNA gene sequence similarity withLeifsonia lichenia2SbT(96.3 %) andHumibacter antriD7-27T(96.2 %). However, phylogenetic analyses showed that strain XATdid not cluster with any species of the generaLeifsonia,Humibacteror other genera of the familyMicrobacteriaceaeand the phylumActinobacteria. Based on 16S rRNA gene sequence analyses, and the phenotypic and chemotaxonomic data, strain XATis considered to represent a novel species of a novel genus, for which the nameCnuibacter physcomitrellaegen. nov., sp. nov. is proposed. The type strain is XAT( = CGMCC 1.15041T= DSM 29843T).
authors.affiliation: College of Life Science, Capital Normal University, Beijing 100048, PRChina.
authors.forename: Xun
authors.initials: X
authors.lastname: Zhou
authors.affiliation: College of Life Science, Capital Normal University, Beijing 100048, PRChina.
authors.forename: Guan
authors.initials: G
authors.lastname: Nan Guo
authors.affiliation: College of Life Science, Capital Normal University, Beijing 100048, PRChina.
authors.forename: Le
authors.initials: L
authors.lastname: Qi Wang
authors.affiliation: College of Life Science, Capital Normal University, Beijing 100048, PRChina.
authors.forename: Su
authors.initials: S
authors.lastname: Lan Bai
authors.affiliation: College of Life Science, Capital Normal University, Beijing 100048, PRChina.
authors.forename: Yan
authors.initials: Y
authors.lastname: Hong Li