title: Click Chemistry Mediated Functionalization of Vertical Nanowires for Biological Applications.
abstract: Semiconductor nanowires (NWs) are gaining significant importance in various biological applications, such as biosensing and drug delivery. Efficient and controlled immobilization of biomolecules on the NW surface is crucial for many of these applications. Here, we present for the first time the use of the Cu(I) -catalyzed alkyne-azide cycloaddition and its strain-promoted variant for the covalent functionalization of vertical NWs with peptides and proteins. The potential of the approach was demonstrated in two complementary applications of measuring enzyme activity and protein binding, which is of general interest for biological studies. The attachment of a peptide substrate provided NW arrays for the detection of protease activity. In addition, green fluorescent protein was immobilized in a site-specific manner and recognized by antibody binding to demonstrate the proof-of-concept for the use of covalently modified NWs for diagnostic purposes using minute amounts of material.
authors.affiliation: Center for Evolutionary Chemical Biology, Department of Chemistry, University of Copenhagen, Universitetsparken 5, 2100 Copenhagen (Denmark).
authors.forename: Surendra
authors.initials: S
authors.lastname: Vutti
authors.affiliation: Center for Evolutionary Chemical Biology, Department of Chemistry, University of Copenhagen, Universitetsparken 5, 2100 Copenhagen (Denmark). sanne@chem.ku.dk.
authors.forename: Sanne
authors.initials: S
authors.lastname: Schoffelen
authors.affiliation: Nano-science Center and Center for Quantum Devices, Niels Bohr Institute, University of Copenhagen, Universitetsparken 5, 2100 Copenhagen (Denmark).
authors.forename: Jessica
authors.initials: J
authors.lastname: Bolinsson
authors.affiliation: Bionanotechnology and Nanobiomedicine Laboratory, Department of Chemistry, University of Copenhagen, Universitetsparken 5, 2100 Copenhagen (Denmark).
authors.forename: Nina
authors.initials: N
authors.lastname: Buch-Månson
authors.affiliation: Nano-Science Center, Department of Chemistry, University of Copenhagen, Universitetsparken 5, 2100 Copenhagen (Denmark).
authors.forename: Nicolas
authors.initials: N
authors.lastname: Bovet
authors.affiliation: Nano-science Center and Center for Quantum Devices, Niels Bohr Institute, University of Copenhagen, Universitetsparken 5, 2100 Copenhagen (Denmark).
authors.forename: Jesper
authors.initials: J
authors.lastname: Nygård
authors.affiliation: Bionanotechnology and Nanobiomedicine Laboratory, Department of Chemistry, University of Copenhagen, Universitetsparken 5, 2100 Copenhagen (Denmark).
authors.forename: Karen L
authors.initials: KL
authors.lastname: Martinez
authors.affiliation: Center for Evolutionary Chemical Biology, Department of Chemistry, University of Copenhagen, Universitetsparken 5, 2100 Copenhagen (Denmark). meldal@nano.ku.dk.
authors.forename: Morten
authors.initials: M
authors.lastname: Meldal
mesh_terms: D000480:Alkynes / Q000737:chemistry*; D001386:Azides / Q000737:chemistry*; D005075:Biological Evolution; D002384:Catalysis; D057930:Click Chemistry; D003300:Copper / Q000737:chemistry*; D061565:Cycloaddition Reaction; D005456:Fluorescent Dyes / Q000737:chemistry*; D049452:Green Fluorescent Proteins / Q000737:chemistry* / Q000378:metabolism; D053770:Nanowires / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D011485:Protein Binding
chemical_list: D000480:Alkynes; D001386:Azides; D005456:Fluorescent Dyes; D010455:Peptides; D049452:Green Fluorescent Proteins; D003300:Copper
keywords: alkyne-azide cycloaddition; biosensors; diazotransfer; nanowires; protein immobilization