title: Effect of prevascularization on in vivo vascularization of poly(propylene fumarate)/fibrin scaffolds.
abstract: The importance of vascularization in the field of bone tissue engineering has been established by previous studies. The present work proposes a novel poly(propylene fumarate) (PPF)/fibrin composite scaffold for the development of vascularized neobone tissue. The effect of prevascularization (i.e., in vitro pre-culture prior to implantation) with human mesenchymal stem cells (hMSCs) and human umbilical vein endothelial cells (HUVECs) on in vivo vascularization of scaffolds was determined. Five conditions were studied: no pre-culture (NP), 1 week pre-culture (1P), 2 week pre-culture (2P), 3 week pre-culture (3P), and scaffolds without cells (control, C). Scaffolds were implanted subcutaneously in a severe combined immunodeficiency (SCID) mouse model for 9 days. During in vitro studies, CD31 staining showed a significant increase in vascular network area over 3 weeks of culture. Vascular density was significantly higher in vivo when comparing the NP and 3P groups. Immunohistochemical staining of human CD-31 expression indicated spreading of vascular networks with increasing pre-culture time. These vascular networks were perfused with mouse blood indicated by perfused lectin staining in human CD-31 positive vessels. Our results demonstrate that in vitro prevascularization supports in vivo vascularization in PPF/fibrin scaffolds.
authors.affiliation: Department of Plastic Surgery, The Ohio State University, Columbus, OH, USA.
authors.forename: Ruchi
authors.initials: R
authors.lastname: Mishra
authors.affiliation: Department of Biomedical Engineering, Illinois Institute of Technology, Chicago, IL, USA; Research Service, Edward Hines Jr. V.A. Hospital, Hines, IL, USA.
authors.forename: Brianna M
authors.initials: BM
authors.lastname: Roux
authors.affiliation: Department of Plastic Surgery, The Ohio State University, Columbus, OH, USA.
authors.forename: Megan
authors.initials: M
authors.lastname: Posukonis
authors.affiliation: Department of Plastic Surgery, The Ohio State University, Columbus, OH, USA.
authors.forename: Emily
authors.initials: E
authors.lastname: Bodamer
authors.affiliation: Department of Biomedical Engineering, Illinois Institute of Technology, Chicago, IL, USA; Research Service, Edward Hines Jr. V.A. Hospital, Hines, IL, USA.
authors.forename: Eric M
authors.initials: EM
authors.lastname: Brey
authors.affiliation: Fischell Department of Bioengineering, University of Maryland, College Park, MD, USA.
authors.forename: John P
authors.initials: JP
authors.lastname: Fisher
authors.affiliation: Department of Plastic Surgery, The Ohio State University, Columbus, OH, USA. Electronic address: David.Dean@osumc.edu.
authors.forename: David
authors.initials: D
authors.lastname: Dean
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D001842:Bone and Bones; D002196:Capillaries / Q000254:growth & development*; D002478:Cells, Cultured; D005337:Fibrin / Q000737:chemistry*; D005650:Fumarates / Q000737:chemistry*; D064593:Heterografts; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D020100:Hydrogels / Q000737:chemistry; D051379:Mice; D016513:Mice, SCID; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D009940:Organoids / Q000098:blood supply*; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000096:biosynthesis; D011126:Polypropylenes / Q000737:chemistry*; D066330:Printing, Three-Dimensional; D018874:Spheroids, Cellular; D013997:Time Factors; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials; D005650:Fumarates; D020100:Hydrogels; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D011126:Polypropylenes; C097850:poly(propylene fumarate); D005337:Fibrin
keywords: Fibrinogen; Human umbilical vein endothelial cells (HUVECs); Mesenchymal stem cells (MSCs); Poly(propylene fumarate) (PPF); Thrombin