title: Mir-181b Functions as Anti-Apoptotic Gene in Post-Status Epilepticus via Modulation of Nrarp and Notch Signaling Pathway.
abstract: BACKGROUND
  The expression pattern and function of miRs in the post-status epilepticus (SE) rat are still unclear. This study aimed to investigate the role and mechanism of miR-181b in the post- SE rat.
  
  
  METHODS
  The lithium-pilocarpine-induced SE model was established in Sprague-Dawley rats. The expression of miR-181b in rat blood and hippocampus was confirmed by RT-PCR at 24 hous and 7 days post-SE. The expression of Nrarp, Hes-1, and Bcl-2 was detected by RT-PCR and western blot. After the rat model was treated with LV-rno-mir181b and LV-anti-181b-5p, the expression change of miR-181b, Nrarp, Hes-1, and Bcl-2 was examined again. The effects of altering the expression of miR-181b on neurone cell apoptosis post-SE were assessed.
  
  
  RESULTS
  The expression of miR-181b significantly decreased both in post-SE rat hippocampus and peripheral blood at 24 hours and 7 days (p<0.05). Nrarp is up-regulated, however, Hes-1 and Bcl-2 are down-regulated (p<0.05). LV-miR-181b treatment induced down-regulation of Nrarp and up-regulation of Hes-1 and Bcl-2 (p<0.05). Neurone cell apoptosis decreased in miR-181b group and post-SE rat hippocampal (p<0.05).
  
  
  CONCLUSIONS
  Our study showed the low-expression of miR-181b in the hippocampus in a post-SE rat model. MiR-181b negatively regulated Nrarp as an anti-apoptotic gene via Notch signaling pathway.
authors.affiliation: Department of Neurology, Third Xiangya Hospital, Central South University, Changsha, China zhangchen1208@hotmail.com.
authors.forename: Chen
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Neurology, Third Xiangya Hospital, Central South University, Changsha, China.
authors.forename: Deren
authors.initials: D
authors.lastname: Hou
authors.affiliation: Department of Neurology, Third Xiangya Hospital, Central South University, Changsha, China.
authors.forename: Xialu
authors.initials: X
authors.lastname: Feng
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D004195:Disease Models, Animal; D005786:Gene Expression Regulation; D006624:Hippocampus / Q000473:pathology / Q000502:physiology*; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D009474:Neurons / Q000473:pathology / Q000502:physiology; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D051880:Receptors, Notch / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000235:genetics; D013226:Status Epilepticus / Q000235:genetics* / Q000378:metabolism / Q000473:pathology
chemical_list: C581717:MIRN181 microRNA, rat; D035683:MicroRNAs; C000611230:Nrarp protein, rat; D011506:Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D051880:Receptors, Notch
keywords: Apoptosis; Hippocampus; MiRNA; Status epilepticus