title: The neural elements in the lining of the ventricular-subventricular zone: making an old story new by high-resolution scanning electron microscopy.
abstract: The classical description of the neural elements that compose the lining of brain ventricles introduces us to the single layer of ependymal cells. However, new findings, especially in the lateral ventricle (LV)-the major niche for the generation of new neurons in the adult brain-have provided information about additional cell elements that influence the organization of this part of the ventricular system and produce important contributions to neurogenesis. To complement the cell neurochemistry findings, we present a three-dimensional in situ description that demonstrates the anatomical details of the different types of ciliated cells and the innervation of these elements. After processing adult rat brains for ultrastructural analysis by high-resolution scanning electron microscopy (HRSEM) and transmission electron microscopy, we observed a heterogeneous pattern of cilia distribution at the different poles of the LV surface. Furthermore, we describe the particular three-dimensional aspects of the ciliated cells of the LV, in addition the fiber bundles and varicose axons surrounding these cells. Therefore, we provide a unique ultrastructural description of the three-dimensional in situ organization of the LV surface, highlighting its innervation, to corroborate the available neurochemical and functional findings regarding the factors that regulate this neurogenic niche.
authors.affiliation: Laboratory of Ultrastructure of Cells and Tissues, Department of Anatomy, Institute of Biomedical Sciences, University of São Paulo São Paulo, Brazil.
authors.forename: Carlos Alexandre Dos Santos
authors.initials: CA
authors.lastname: Haemmerle
authors.affiliation: Laboratory of Neurosciences, Department of Anatomy, Institute of Biomedical Sciences, University of São Paulo São Paulo, Brazil ; Institute of Psychology, Center for Neuroscience and Behavior, University of São Paulo São Paulo, Brazil.
authors.forename: Maria Inês
authors.initials: MI
authors.lastname: Nogueira
authors.affiliation: Laboratory of Ultrastructure of Cells and Tissues, Department of Anatomy, Institute of Biomedical Sciences, University of São Paulo São Paulo, Brazil.
authors.forename: Ii-Sei
authors.initials: IS
authors.lastname: Watanabe
keywords: axon; ependymal cell; lateral ventricle; neurogenesis; olfactory bulb; primary cilium; subventricular zone