title: [Background Level of γH2AX Foci in Human Cells as a Factor of Individual Radiosensitivity].
abstract: The paper analyzes the effects of spontaneous level of yH2AX and 53BP1 foci on a frequency of radiation-induced centromere-negative and centromere-positive micronuclei in peripheral blood lymphocytes of 54 healthy individuals after exposure to 2 Gy of ionizing radiation in vitro. An inverse correlation was found between the level of spontaneous yH2AX foci and the frequency of centromere-negative micronuclei after irradiation. The corresponding correlations between the spontaneous level of protein 53BP1 foci and the frequency of centromere-negative micronuclei were not statistically significant. In addition, cells of the individuals with a high frequency of radiation-induced micronuclei were also characterized by a low proliferative activity. It was suggested that DNA double strand break repair works less efficiently in cells of the individuals with low levels of spontaneous yH2AX foci, and a greater number of DNA double strand breaks after exposure to ionizing radiation remains unrepaired, thus leading to a cell cycle block and an increase of the frequency of centromere-negative micronuclei.
authors.forename: S A
authors.initials: SA
authors.lastname: Vasilyev
authors.forename: A I
authors.initials: AI
authors.lastname: Velichevskaya
authors.forename: T V
authors.initials: TV
authors.lastname: Vishnevskaya
authors.forename: A A
authors.initials: AA
authors.lastname: Belenko
authors.forename: O V
authors.initials: OV
authors.lastname: Gribova
authors.forename: M B
authors.initials: MB
authors.lastname: Plaksin
authors.forename: Zh A
authors.initials: ZhA
authors.lastname: Startseva
authors.forename: I N
authors.initials: IN
authors.lastname: Lebedev
mesh_terms: D000328:Adult; D000368:Aged; D002478:Cells, Cultured; D053903:DNA Breaks, Double-Stranded; D004260:DNA Repair; D005720:Gamma Rays / Q000009:adverse effects; D006657:Histones / Q000235:genetics*; D006801:Humans; D008214:Lymphocytes / Q000473:pathology / Q000528:radiation effects*; D008297:Male; D048629:Micronuclei, Chromosome-Defective / Q000528:radiation effects*; D015162:Micronucleus Tests; D008875:Middle Aged; D011836:Radiation Tolerance / Q000235:genetics*; D055815:Young Adult
chemical_list: C468783:H2AX protein, human; D006657:Histones