title: Folic acid-conjugated fluorescent polymer for up-regulation folate receptor expression study via targeted imaging of tumor cells.
abstract: Thoroughly investigation of folate receptor (FR) expression related to targeting drug delivery in tumor cells has been intensively pursued in recent years. Herein, a simple and versatile strategy for determination of FR expression based on targeted imaging of tumor cells with fluorescent nano-conjugates was developed. The fluorescent nano-conjugates were composed of poly 2-vinyl-4,4-dimethyl azlactone (PVDMA) as the linker, folic acid as the targeting unit and amino-Rhodamine B as the fluorescent ligand. Owing to possessing dimethyl azlactone groups in polymer framework, PVDMA could easily reacted with amines or alcohols, and form water soluble materials. Fluorescent imaging studies indicated that the prepared nano-conjugates could specifically target tumor cells and monitor the over expressing of FR. Moreover, the FR expression up-regulation in HeLa cells through medicines regulation has been further explored. This new protocol opens an effective way through synthesis and design of novel fluorescent nano-conjugates for FR expression investigation in tumor cells via targeted imaging, showing great potential in drug delivery mechanism study and cancer therapy.
authors.affiliation: Beijing National Laboratory for Molecular Sciences; Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, 100190 Beijing, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Qiao
authors.affiliation: Research Centre of Plastic Surgery Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, 100144 Beijing, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Dong
authors.affiliation: Beijing National Laboratory for Molecular Sciences; Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, 100190 Beijing, China; Graduate School, University of Chinese Academy of Sciences, 100049 Beijing, China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Mu
authors.affiliation: Beijing National Laboratory for Molecular Sciences; Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, 100190 Beijing, China. Electronic address: qili@iccas.ac.cn.
authors.forename: Li
authors.initials: L
authors.lastname: Qi
authors.affiliation: Research Centre of Plastic Surgery Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, 100144 Beijing, China.
authors.forename: Ran
authors.initials: R
authors.lastname: Xiao
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry; D058975:Folate Receptor 1 / Q000096:biosynthesis / Q000302:isolation & purification*; D005492:Folic Acid / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D007783:Lactones / Q000737:chemistry; D049329:Nanostructures / Q000737:chemistry*; D011145:Polyvinyls / Q000737:chemistry; D012235:Rhodamines / Q000737:chemistry
chemical_list: D005456:Fluorescent Dyes; D058975:Folate Receptor 1; D007783:Lactones; D011145:Polyvinyls; D012235:Rhodamines; C539182:poly(2-vinyl-4,4-dimethyl azlactone); D005492:Folic Acid; C029773:rhodamine B
keywords: Folate receptor expression; Polymer-based fluorescent nano-conjugates; Targeted imaging; Up-regulation folate receptor