title: A surface acoustic wave biosensor for interrogation of single tumour cells in microcavities.
abstract: In this study, biological cells are sensed and characterized with surface acoustic wave (SAW) devices utilising microcavities. After tumour cells in media are transported to and trapped in microcavities, the proposed platform uses SAW interaction between the substrate and the cells to extract their mechanical stiffness based on the ultrasound velocity. Finite element method (FEM) analysis and experimental results show that output phase information is an indicator of the stiffness modulus of the trapped cells. Small populations of various types of cells such as MCF7, MDA-MB-231, SKBR3, and JJ012 were characterized and characteristic moduli were estimated for each cell population. Results show that high frequency stiffness modulus is a possible biomarker for aggressiveness of the tumour and that microcavity coupled SAW devices are a good candidate for non-invasive interrogation of single cells.
authors.affiliation: Electrical and Computer Engineering Department, University of Miami, Coral Gables, FL 33146, USA. senveli@umiami.edu tigli@miami.edu.
authors.forename: Sukru U
authors.initials: SU
authors.lastname: Senveli
authors.forename: Zheng
authors.initials: Z
authors.lastname: Ao
authors.forename: Siddarth
authors.initials: S
authors.lastname: Rawal
authors.forename: Ram H
authors.initials: RH
authors.lastname: Datar
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Cote
authors.forename: Onur
authors.initials: O
authors.lastname: Tigli
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation; D045744:Cell Line, Tumor; D020342:Finite Element Analysis; D006801:Humans; D055617:Micro-Electrical-Mechanical Systems / Q000295:instrumentation; D010316:Particle Size; D059010:Single-Cell Analysis / Q000295:instrumentation / Q000379:methods*; D013016:Sound; D013499:Surface Properties