title: Simultaneous determination of nineteen major components in Qi She Pill by ultra-high-performance liquid chromatography-tandem mass spectrometry.
abstract: Qi She Pill (QSP) is a traditional Chinese medicine (TCM) prescription that has been used in treating cervical spondylosis radiculopathy for many years. In this study, a simple and sensitive method using ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) on a reverse-phase C18 column was developed for the simultaneous determination of the 19 major components in QSP. We found that the optimum mobile phase for gradient elution was 0.1% formic acid and methanol. The correlation coefficients of all calibration curves were greater than 0.99. Recoveries measured at three concentration levels varied from 95.43% to 102.35%. Relative standard deviations of intra- and inter-day precisions were less than 4.45%. After successfully validating our method, we then applied it to the quantification of 19 components in QSP products to show that this method provides a new standard in quality assessment of TCM prescriptions containing multiple bioactive components.
authors.affiliation: Experimental Center for Science and Technology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
authors.forename: Zhongliang
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Experimental Center for Science and Technology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Li
authors.affiliation: Experimental Center for Science and Technology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
authors.forename: Qiufen
authors.initials: Q
authors.lastname: Li
authors.affiliation: Experimental Center for Science and Technology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
authors.forename: Simiao
authors.initials: S
authors.lastname: Du
authors.affiliation: Experimental Center for Science and Technology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
authors.forename: Yongquan
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Experimental Center for Science and Technology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
authors.forename: Chunming
authors.initials: C
authors.lastname: Lv
authors.affiliation: Experimental Center for Science and Technology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Institute of Spine, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China.
authors.forename: Yongjun
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Experimental Center for Science and Technology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Zhang
keywords: 5-O-M, 5-O-methylvisammioside; AST-III, astragaloside III; AST-IV, astragaloside IV; Active components; BER, berberine; CA, cholic acid; CCS, calycosin; CCSG, calycosin-7-O-β-d-glucoside; CE, collision energy; CSR, cervical spondylosis radiculopathy; DAI, daidzein; FAN, fangchinoline; FOR, formononetin; GA, gallic acid; ONO, ononin; PAL, palmatine; QSP, Qi She Pill; Qi She Pill (QSP); RA, rheumatoid arthritis; SEA, senkyunolide A; SEI, senkyunolide I; SIN, sinomenine; SRM, selective reaction monitoring; TCM, traditional Chinese medicine; TET, (+)-tetrandrine; THP, tetrahydropalmatine; THPB, tetrahydroepiberberine; Traditional Chinese medicine; UHPLC–MS/MS