title: Core-shell-corona doxorubicin-loaded superparamagnetic Fe3O4 nanoparticles for cancer theranostics.
abstract: Superparamagnetic iron oxide magnetic nanoparticles (MNPs) are successfully used as contrast agents in magnetic-resonance imaging. They can be easily functionalized for drug delivery functions, demonstrating great potential for both imaging and therapeutic applications. Here we developed new pH-responsive theranostic core-shell-corona nanoparticles consisting of superparamagentic Fe3O4 core that displays high T2 relaxivity, bovine serum albumin (BSA) shell that binds anticancer drug, doxorubicin (Dox) and poly(ethylene glycol) (PEG) corona that increases stability and biocompatibility. The nanoparticles were produced by adsorption of the BSA shell onto the Fe3O4 core followed by crosslinking of the protein layer and subsequent grafting of the PEG corona using monoamino-terminated PEG via carbodiimide chemistry. The hydrodynamic diameter, zeta-potential, composition and T2 relaxivity of the resulting nanoparticles were characterized using transmission electron microscopy, dynamic light scattering, thermogravimetric analysis and T2-relaxometry. Nanoparticles were shown to absorb Dox molecules, possibly through a combination of electrostatic and hydrophobic interactions. The loading capacity (LC) of the nanoparticles was 8 wt.%. The Dox loaded nanoparticles release the drug at a higher rate at pH 5.5 compared to pH 7.4 and display similar cytotoxicity against C6 and HEK293 cells as the free Dox.
authors.affiliation: Pirogov Russian National Research Medical University, Ostrovitianov 1, 117997 Moscow, Russia.
authors.forename: A
authors.initials: A
authors.lastname: Semkina
authors.affiliation: Pirogov Russian National Research Medical University, Ostrovitianov 1, 117997 Moscow, Russia; Lomonosov Moscow State University, Lenin Hills 1, 119991 Moscow, Russia. Electronic address: abakumov1988@gmail.com.
authors.forename: M
authors.initials: M
authors.lastname: Abakumov
authors.affiliation: Serbsky Federal Medical Research Centre of Psychiatry and Narcology, Kropotkinskiy 23, 119991 Moscow, Russia.
authors.forename: N
authors.initials: N
authors.lastname: Grinenko
authors.affiliation: Electron Microscopy for Materials Science (EMAT), University of Antwerp, Groenenborgerlaan 171, 2020 Antwerp, Belgium.
authors.forename: A
authors.initials: A
authors.lastname: Abakumov
authors.affiliation: Lomonosov Moscow State University, Lenin Hills 1, 119991 Moscow, Russia.
authors.forename: A
authors.initials: A
authors.lastname: Skorikov
authors.affiliation: Federal State Institution of Science Institute of Theoretical and Experimental Biophysics, Russian Academy of Science, Institutskaya 3, 142290 Pushchino, Russia.
authors.forename: E
authors.initials: E
authors.lastname: Mironova
authors.affiliation: Federal State Institution of Science Institute of Theoretical and Experimental Biophysics, Russian Academy of Science, Institutskaya 3, 142290 Pushchino, Russia.
authors.forename: G
authors.initials: G
authors.lastname: Davydova
authors.affiliation: Lomonosov Moscow State University, Lenin Hills 1, 119991 Moscow, Russia.
authors.forename: A G
authors.initials: AG
authors.lastname: Majouga
authors.affiliation: Lomonosov Moscow State University, Lenin Hills 1, 119991 Moscow, Russia; Serbsky Federal Medical Research Centre of Psychiatry and Narcology, Kropotkinskiy 23, 119991 Moscow, Russia.
authors.forename: N
authors.initials: N
authors.lastname: Nukolova
authors.affiliation: Lomonosov Moscow State University, Lenin Hills 1, 119991 Moscow, Russia; University of North Carolina at Chapel Hill, Center for Nanotechnology in Drug Delivery, 125 Mason Farm Road, Chapel Hill 27599-7362 NC, USA.
authors.forename: A
authors.initials: A
authors.lastname: Kabanov
authors.affiliation: Pirogov Russian National Research Medical University, Ostrovitianov 1, 117997 Moscow, Russia; Serbsky Federal Medical Research Centre of Psychiatry and Narcology, Kropotkinskiy 23, 119991 Moscow, Russia.
authors.forename: V
authors.initials: V
authors.lastname: Chekhonin
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000008:administration & dosage*; D045744:Cell Line, Tumor; D004317:Doxorubicin / Q000008:administration & dosage*; D052203:Ferrosoferric Oxide / Q000737:chemistry*; D006801:Humans; D053768:Metal Nanoparticles; D046529:Microscopy, Electron, Transmission; D009369:Neoplasms / Q000175:diagnosis* / Q000628:therapy*; D000068936:Theranostic Nanomedicine
chemical_list: D000903:Antibiotics, Antineoplastic; D004317:Doxorubicin; D052203:Ferrosoferric Oxide
keywords: Anticancer therapy; Doxorubicin; Drug delivery; MRI-contrast agents; Magnetic nanoparticles