title: Large-Scale Glycomics of Livestock: Discovery of Highly Sensitive Serum Biomarkers Indicating an Environmental Stress Affecting Immune Responses and Productivity of Holstein Dairy Cows.
abstract: Because various stresses strongly influence the food productivity of livestock, biomarkers to indicate unmeasurable environmental stress in domestic animals are of increasing importance. Thermal comfort is one of the basic principles of dairy cow welfare that enhances productivity. To discover sensitive biomarkers that monitor such environmental stresses in dairy cows, we herein performed, for the first time, large-scale glycomics on 336 lactating Holstein cow serum samples over 9 months between February and October. Glycoblotting combined with MALDI-TOF/MS and DMB/HPLC allowed for comprehensive glycomics of whole serum glycoproteins. The results obtained revealed seasonal alterations in serum N-glycan levels and their structural characteristics, such as an increase in high-mannose type N-glycans in spring, the occurrence of di/triantennary complex type N-glycans terminating with two or three Neu5Gc residues in summer and autumn, and N-glycans in winter dominantly displaying Neu5Ac. A multivariate analysis revealed a correlation between the serum expression levels of these season-specific glycoforms and productivity.
authors.affiliation: Faculty of Advanced Life Science and Graduate School of Life Science, Hokkaido University , N21, W11, Kita-ku, Sapporo 001-0021, Japan.|Animal Behaviour and Management Department, Faculty of Veterinary Medicine, South Valley University , Qena 83523, Egypt.
authors.forename: Ibrahim F
authors.initials: IF
authors.lastname: Rehan
authors.affiliation: Animal Production System, Graduate School of Agriculture, Hokkaido University , N9, W9, Sapporo 060-8589, Japan.
authors.forename: Koichiro
authors.initials: K
authors.lastname: Ueda
authors.affiliation: Animal Production System, Graduate School of Agriculture, Hokkaido University , N9, W9, Sapporo 060-8589, Japan.
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Mitani
authors.affiliation: Faculty of Advanced Life Science and Graduate School of Life Science, Hokkaido University , N21, W11, Kita-ku, Sapporo 001-0021, Japan.
authors.forename: Maho
authors.initials: M
authors.lastname: Amano
authors.affiliation: Faculty of Advanced Life Science and Graduate School of Life Science, Hokkaido University , N21, W11, Kita-ku, Sapporo 001-0021, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hinou
authors.affiliation: Faculty of Advanced Life Science and Graduate School of Life Science, Hokkaido University , N21, W11, Kita-ku, Sapporo 001-0021, Japan.|Medicinal Chemistry Pharmaceuticals Co., Ltd., N21, W12, Kita-ku, Sapporo 001-0021, Japan.
authors.forename: Tetsu
authors.initials: T
authors.lastname: Ohashi
authors.affiliation: Animal Production System, Graduate School of Agriculture, Hokkaido University , N9, W9, Sapporo 060-8589, Japan.
authors.forename: Seiji
authors.initials: S
authors.lastname: Kondo
authors.affiliation: Faculty of Advanced Life Science and Graduate School of Life Science, Hokkaido University , N21, W11, Kita-ku, Sapporo 001-0021, Japan.|Medicinal Chemistry Pharmaceuticals Co., Ltd., N21, W12, Kita-ku, Sapporo 001-0021, Japan.
authors.forename: Shin-Ichiro
authors.initials: S
authors.lastname: Nishimura
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood*; D002417:Cattle / Q000097:blood* / Q000235:genetics / Q000276:immunology / Q000502:physiology; D002851:Chromatography, High Pressure Liquid; D005260:Female; D054794:Glycomics; D006023:Glycoproteins / Q000097:blood* / Q000276:immunology; D007774:Lactation; D058751:Livestock / Q000097:blood / Q000235:genetics / Q000276:immunology / Q000502:physiology; D008892:Milk / Q000378:metabolism; D053719:Tandem Mass Spectrometry
chemical_list: D015415:Biomarkers; D006023:Glycoproteins
keywords: Holstein dairy cow welfare; N-glycan structure; environmental stress; glycoblotting; immune responses; serum biomarker; serum glycoproteins; sialic acids