title: Molecular characterization of Urocleidoides cuiabai and U. malabaricusi (Monogenea: Dactylogyridae) from the trahira fish Hoplias aff. malabaricus in the Paraná River, Brazil.
abstract: Urocleidoides ectoparasites are mainly found on fish of the neotropical regions. Although molecular research on monogeneans is available, no genetic data exist characterizing species in the Urocleidoides genus. Some DNA sequences have been efficacious in systematic studies and in the reconstruction of phylogenies of fish parasites. Relevant roles have been given to the sequence of the mitochondrial gene of cytochrome c oxidase I (COI). This study characterized COI sequences of the parasites Urocleidoides malabaricusi and U. cuiabai in trahira fish Hoplias aff. malabaricus of the flood plain of the Upper River Paraná, Brazil. The two species under analysis were distinguished by sequencing and analysing a 420-bp fragment of the COI gene, which suggested the existence of the cryptic species U. malabaricusi.
authors.affiliation: Instituto de Ciências Biológicas, Médicas e da Saúde, Universidade Paranaense,Praça Mascarenhas de Moraes 4282,Umuarama,PR,CEP 87502-210,Brazil.
authors.forename: L S
authors.initials: LS
authors.lastname: Gasques
authors.affiliation: Programa de pós graduação em Biologia Comparada, Centro de Ciências Biológicas, Universidade Estadual de Maringá,Av. Colombo, 5790, Jardim Universitário,Maringá,PR,CEP 87020-900,Brazil.
authors.forename: R J
authors.initials: RJ
authors.lastname: Graça
authors.affiliation: Núcleo de Pesquisas em Limnologia, Ictiologia e Aquicultura (Nupélia), Universidade Estadual de Maringá,Av. Colombo 5790, Bloco G-90,Maringá,PR,CEP 87020-900,Brazil.
authors.forename: S M A P
authors.initials: SM
authors.lastname: Prioli
authors.affiliation: Núcleo de Pesquisas em Limnologia, Ictiologia e Aquicultura (Nupélia), Universidade Estadual de Maringá,Av. Colombo 5790, Bloco G-90,Maringá,PR,CEP 87020-900,Brazil.
authors.forename: R M
authors.initials: RM
authors.lastname: Takemoto
authors.affiliation: Núcleo de Pesquisas em Limnologia, Ictiologia e Aquicultura (Nupélia), Universidade Estadual de Maringá,Av. Colombo 5790, Bloco G-90,Maringá,PR,CEP 87020-900,Brazil.
authors.forename: A J
authors.initials: AJ
authors.lastname: Prioli
mesh_terms: D000818:Animals; D001938:Brazil; D002590:Cestode Infections / Q000469:parasitology / Q000662:veterinary*; D060125:Characiformes / Q000469:parasitology*; D016000:Cluster Analysis; D003576:Electron Transport Complex IV / Q000235:genetics; D014644:Genetic Variation; D010802:Phylogeny; D010986:Platyhelminths / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D045483:Rivers; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
chemical_list: D003576:Electron Transport Complex IV