title: Antiviral Activity of Porcine Interferon Regulatory Factor 1 against Swine Viruses in Cell Culture.
abstract: Interferon regulatory factor 1 (IRF1), as an important transcription factor, is abundantly induced upon virus infections and participates in host antiviral immune responses. However, the roles of porcine IRF1 (poIRF1) in host antiviral defense remain poorly understood. In this study, we determined that poIRF1 was upregulated upon infection with viruses and distributed in nucleus in porcine PK-15 cells. Subsequently, we tested the antiviral activities of poIRF1 against several swine viruses in cells. Overexpression of poIRF1 can efficiently suppress the replication of viruses, and knockdown of poIRF1 promotes moderately viral replication. Interestingly, overexpression of poIRF1 enhances dsRNA-induced IFN-β and IFN-stimulated response element (ISRE) promoter activation, whereas knockdown of poIRF1 cannot significantly affect the activation of IFN-β promoter induced by RNA viruses. This study suggests that poIRF1 plays a significant role in cellular antiviral response against swine viruses, but might be dispensable for IFN-β induction triggered by RNA viruses in PK-15 cells. Given these results, poIRF1 plays potential roles in cellular antiviral responses against swine viruses.
authors.affiliation: College of Animal Husbandry &amp; Veterinary Science, Henan Agricultural University, Zhengzhou 450002, China. yongtaole@126.com.
authors.forename: Yongtao
authors.initials: Y
authors.lastname: Li
authors.affiliation: College of Animal Husbandry &amp; Veterinary Science, Henan Agricultural University, Zhengzhou 450002, China. ndcht@163.com.
authors.forename: Hongtao
authors.initials: H
authors.lastname: Chang
authors.affiliation: College of Animal Husbandry &amp; Veterinary Science, Henan Agricultural University, Zhengzhou 450002, China. liyihan2001@163.com.
authors.forename: Xia
authors.initials: X
authors.lastname: Yang
authors.affiliation: College of Animal Husbandry &amp; Veterinary Science, Henan Agricultural University, Zhengzhou 450002, China. zyx0920@163.com.
authors.forename: Yongxiang
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: College of Animal Husbandry &amp; Veterinary Science, Henan Agricultural University, Zhengzhou 450002, China. chenluhau@sina.com.
authors.forename: Lu
authors.initials: L
authors.lastname: Chen
authors.affiliation: College of Animal Husbandry &amp; Veterinary Science, Henan Agricultural University, Zhengzhou 450002, China. xinweicliff@126.com.
authors.forename: Xinwei
authors.initials: X
authors.lastname: Wang
authors.affiliation: College of Animal Husbandry &amp; Veterinary Science, Henan Agricultural University, Zhengzhou 450002, China. ndlhy@163.com.
authors.forename: Hongying
authors.initials: H
authors.lastname: Liu
authors.affiliation: College of Animal Husbandry &amp; Veterinary Science, Henan Agricultural University, Zhengzhou 450002, China. wchuanq@163.com.
authors.forename: Chuanqing
authors.initials: C
authors.lastname: Wang
authors.affiliation: College of Animal Husbandry &amp; Veterinary Science, Henan Agricultural University, Zhengzhou 450002, China. zhaoj@henau.edu.cn.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhao
mesh_terms: D000818:Animals; D002460:Cell Line; D004847:Epithelial Cells / Q000276:immunology* / Q000821:virology*; D005786:Gene Expression Regulation; D054884:Host-Pathogen Interactions; D050856:Interferon Regulatory Factor-1 / Q000378:metabolism*; D013552:Swine; D015854:Up-Regulation; D014779:Virus Replication; D014780:Viruses / Q000276:immunology*
chemical_list: D050856:Interferon Regulatory Factor-1
keywords: IFN-stimulated genes; IFN-β; antiviral activity; porcine IRF1