title: TLR2/MyD88/NF-κB signalling pathway regulates IL-8 production in porcine alveolar macrophages infected with porcine circovirus 2.
abstract: Porcine circovirus 2 (PCV2) is the primary cause of post-weaning multisystemic wasting syndrome, in which it stimulates a strong IL-8 response that is associated with chronic inflammation as well as lesions in the lymphoid organs. However, the mechanism underlying PCV2-induced IL-8 production is still unclear. In the present study, we demonstrated that increased IL-8 expression during PCV2 infection depends on Toll-like receptor (TLR2), but not TLR4 or TLR9 signalling pathways in porcine alveolar macrophages. Moreover, we found that impairment of the MyD88/NF-κB signalling pathway by MyD88 knockdown or NF-κB inhibitors markedly decreased PCV2-induced IL-8 secretion. These results suggest that PCV2 induces IL-8 secretion via the TLR2/MyD88/NF-κB signalling pathway. Therefore, it is important to elucidate the molecular mechanisms of the PCV2-induced inflammatory response.
authors.affiliation: College of Veterinary Medicine, China Agricultural University, 2 Yuan-Ming-Yuan West Road, Beijing 100193, PR China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Qin
authors.affiliation: Tianjin Institute of Animal Husbandry and Veterinary Medicine, Jin-Jing Road, Tianjin 300381, PR China.
authors.forename: Haihua
authors.initials: H
authors.lastname: Li
authors.affiliation: Tianjin Institute of Animal Husbandry and Veterinary Medicine, Jin-Jing Road, Tianjin 300381, PR China.
authors.forename: Jiayun
authors.initials: J
authors.lastname: Qiao
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D017925:Circovirus / Q000276:immunology* / Q000502:physiology; D054884:Host-Pathogen Interactions; D016209:Interleukin-8 / Q000096:biosynthesis*; D016676:Macrophages, Alveolar / Q000276:immunology* / Q000821:virology*; D053594:Myeloid Differentiation Factor 88 / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism*; D015398:Signal Transduction; D013552:Swine; D051195:Toll-Like Receptor 2 / Q000378:metabolism*
chemical_list: D016209:Interleukin-8; D053594:Myeloid Differentiation Factor 88; D016328:NF-kappa B; D051195:Toll-Like Receptor 2