title: Exploring the environmental diversity of kinetoplastid flagellates in the high-throughput DNA sequencing era.
abstract: The class Kinetoplastea encompasses both free-living and parasitic species from a wide range of hosts. Several representatives of this group are responsible for severe human diseases and for economic losses in agriculture and livestock. While this group encompasses over 30 genera, most of the available information has been derived from the vertebrate pathogenic genera Leishmaniaand Trypanosoma. Recent studies of the previously neglected groups of Kinetoplastea indicated that the actual diversity is much higher than previously thought. This article discusses the known segment of kinetoplastid diversity and how gene-directed Sanger sequencing and next-generation sequencing methods can help to deepen our knowledge of these interesting protists.
authors.affiliation: Laboratório de Estudos Integrados em Protozoologia, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil.
authors.forename: Claudia Masini
authors.initials: CM
authors.lastname: d'Avila-Levy
authors.affiliation: Laboratório de Estudos Integrados em Protozoologia, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil.
authors.forename: Carolina
authors.initials: C
authors.lastname: Boucinha
authors.affiliation: Life Science Research Centre, University of Ostrava, Ostrava, Czech Republic.
authors.forename: Alexei
authors.initials: A
authors.lastname: Kostygov
authors.affiliation: Laboratório de Estudos Integrados em Protozoologia, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil.
authors.forename: Helena Lúcia Carneiro
authors.initials: HL
authors.lastname: Santos
authors.affiliation: Laboratório de Estudos Integrados em Protozoologia, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil.
authors.forename: Karina Alessandra
authors.initials: KA
authors.lastname: Morelli
authors.affiliation: Life Science Research Centre, University of Ostrava, Ostrava, Czech Republic.
authors.forename: Anastasiia
authors.initials: A
authors.lastname: Grybchuk-Ieremenko
authors.affiliation: Centre National de la Recherche Scientifique, Muséum National d'Histoire Naturelle, Sorbonne Universités, Paris, France.
authors.forename: Linda
authors.initials: L
authors.lastname: Duval
authors.affiliation: Biology Centre, Institute of Parasitology, Czech Academy of Sciences, České Budejovice, Czech Republic.
authors.forename: Jan
authors.initials: J
authors.lastname: Votýpka
authors.affiliation: Life Science Research Centre, University of Ostrava, Ostrava, Czech Republic.
authors.forename: Vyacheslav
authors.initials: V
authors.lastname: Yurchenko
authors.affiliation: Centre National de la Recherche Scientifique, Muséum National d'Histoire Naturelle, Sorbonne Universités, Paris, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Grellier
authors.affiliation: Biology Centre, Institute of Parasitology, Czech Academy of Sciences, České Budejovice, Czech Republic.
authors.forename: Julius
authors.initials: J
authors.lastname: Lukeš
mesh_terms: D044822:Biodiversity; D015415:Biomarkers; D019295:Computational Biology; D058893:DNA Barcoding, Taxonomic / Q000639:trends; D016054:DNA, Protozoan / Q000235:genetics*; D030541:Databases, Genetic; D004777:Environment; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D016830:Kinetoplastida / Q000145:classification / Q000166:cytology / Q000235:genetics*; D056186:Metagenomics / Q000639:trends; D010802:Phylogeny; D016053:RNA, Protozoan / Q000235:genetics*; D012337:RNA, Ribosomal, 18S / Q000235:genetics
chemical_list: D015415:Biomarkers; D016054:DNA, Protozoan; D016053:RNA, Protozoan; D012337:RNA, Ribosomal, 18S