title: Synthesis and characterization of a novel pH-thermo dual responsive hydrogel based on salecan and poly(N,N-diethylacrylamide-co-methacrylic acid).
abstract: Salecan is a water-soluble microbial polysaccharide produced by Agrobacterium sp. ZX09, a salt-tolerant strain isolated from a soil sample in our laboratory. Previous work inspired us salecan is a good candidate to fabricate hydrogels. Poly(N,N-diethylacrylamide) is one type of thermo sensitive polymer which is not investigated extremely as poly(N-isopropylacrylamide). Here, we report a novel pH-thermo dual responsive hydrogel based on salecan and poly(N,N-diethylacrylamide-co-methacrylic acid) semi-interpenetrating polymer networks (semi-IPNs). The physicochemical property of this hydrogel was investigated by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analyses (TGA), rheological test and Scanning Electron Microscopy (SEM). It was interesting that the storage modulus (G') and pore size of the hydrogel could be tuned by adjusting the content of salecan and crosslinker. The pH-thermo dual responsive property was demonstrated by swelling behavior test: the swelling ratio of the hydrogel decreased continuously as the temperature increased from 25 °C to 37 °C, while it was pH-dependent as well. Especially, when exposed to a higher temperature (37 °C) and acidic environment (pH 4.0), drug-loaded hydrogel would have a quick release. Finally, the cytotoxicity of drug-free hydrogels was investigated on A549 and HepG2 cells, results showed that it was non-toxic while the DOX released from hydrogels had comparable cytotoxicity with respect to free DOX. In conclusion, the novel salecan/poly(N,N-diethylacrylamide-co-methacrylic acid) semi-interpenetrating polymer network hydrogels were pH-thermo dual responsive and may be a promising candidate for drug delivery system.
authors.affiliation: Center for Molecular Metabolism, Nanjing University of Science & Technology, Nanjing 210094, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wei
authors.affiliation: Center for Molecular Metabolism, Nanjing University of Science & Technology, Nanjing 210094, China.
authors.forename: Xiaoliang
authors.initials: X
authors.lastname: Qi
authors.affiliation: Center for Molecular Metabolism, Nanjing University of Science & Technology, Nanjing 210094, China.
authors.forename: Yucheng
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Center for Molecular Metabolism, Nanjing University of Science & Technology, Nanjing 210094, China.
authors.forename: Junjian
authors.initials: J
authors.lastname: Li
authors.affiliation: Center for Molecular Metabolism, Nanjing University of Science & Technology, Nanjing 210094, China.
authors.forename: Xinyu
authors.initials: X
authors.lastname: Hu
authors.affiliation: Center for Molecular Metabolism, Nanjing University of Science & Technology, Nanjing 210094, China.
authors.forename: Gancheng
authors.initials: G
authors.lastname: Zuo
authors.affiliation: Center for Molecular Metabolism, Nanjing University of Science & Technology, Nanjing 210094, China.
authors.forename: Jianfa
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Center for Molecular Metabolism, Nanjing University of Science & Technology, Nanjing 210094, China. Electronic address: weidong@njust.edu.cn.
authors.forename: Wei
authors.initials: W
authors.lastname: Dong
mesh_terms: D000178:Acrylamides / Q000737:chemistry*; D020100:Hydrogels; D006863:Hydrogen-Ion Concentration; D008689:Methacrylates / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D012212:Rheology; D017550:Spectroscopy, Fourier Transform Infrared; D013818:Thermogravimetry; D014961:X-Ray Diffraction; D047071:beta-Glucans / Q000737:chemistry*
chemical_list: D000178:Acrylamides; D020100:Hydrogels; D008689:Methacrylates; D011108:Polymers; D047071:beta-Glucans; C428028:poly(N,N-diethylacrylamide); C577623:salecan
keywords: Doxorubicin; Drug delivery; Poly(N,N-Diethylacrylamide); Salecan; Semi-IPN hydrogels