title: Influence of lipidation on the mode of action of a small RW-rich antimicrobial peptide.
abstract: Antimicrobial peptides are a potent class of antibiotics. In the Gram-positive model organism Bacillus subtilis the synthetic peptide RWRWRW-NH2 integrates into the bacterial membrane and delocalizes essential peripheral membrane proteins involved in cell wall biosynthesis and respiration. A lysine residue has been added to the hexapeptide core structure, either C or N-terminally. Lipidation of the lysine residues by a C8-acyl chain significantly improved antibacterial activity against both Gram-positive and Gram-negative bacteria. Here, we report a comparative proteomic study in B. subtilis on the mechanism of action of the lipidated and non-lipidated peptides. All derivatives depolarized the bacterial membrane without forming pores and all affected cell wall integrity. Proteomic profiling of the bacterial stress responses to the small RW-rich antimicrobial peptides was reflective of non-disruptive membrane integration. Overall, our results indicate that antimicrobial peptides can be derivatized with lipid chains enhancing antibacterial activity without significantly altering the mechanism of action. This article is part of a Special Issue entitled: Antimicrobial peptides edited by Karl Lohner and Kai Hilpert.
authors.affiliation: Applied Microbiology, Ruhr University Bochum, Germany.
authors.forename: Michaela
authors.initials: M
authors.lastname: Wenzel
authors.affiliation: Applied Microbiology, Ruhr University Bochum, Germany.
authors.forename: Patrick
authors.initials: P
authors.lastname: Schriek
authors.affiliation: Applied Microbiology, Ruhr University Bochum, Germany.
authors.forename: Pascal
authors.initials: P
authors.lastname: Prochnow
authors.affiliation: Bioinorganic Chemistry, Ruhr University Bochum, Germany.
authors.forename: H Bauke
authors.initials: HB
authors.lastname: Albada
authors.affiliation: Bioinorganic Chemistry, Ruhr University Bochum, Germany.
authors.forename: Nils
authors.initials: N
authors.lastname: Metzler-Nolte
authors.affiliation: Applied Microbiology, Ruhr University Bochum, Germany. Electronic address: julia.bandow@rub.de.
authors.forename: Julia E
authors.initials: JE
authors.lastname: Bandow
mesh_terms: D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D001120:Arginine / Q000737:chemistry / Q000378:metabolism; D001412:Bacillus subtilis / Q000737:chemistry / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002462:Cell Membrane / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D002473:Cell Wall / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D024901:Drug Resistance, Multiple, Bacterial / Q000187:drug effects; D004926:Escherichia coli / Q000737:chemistry / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D013329:Structure-Activity Relationship; D014364:Tryptophan / Q000737:chemistry / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D001426:Bacterial Proteins; D014364:Tryptophan; D001120:Arginine
keywords: Acylation; Antibacterial peptides; Antibiotics; Bacterial membranes; Bacterial stress response; Proteomics