title: Prognostic Factors for Breast Cancer: an Immunomorphological Update.
abstract: The prognostic variability recorded within homogeneous groups of patients for anatomo-clinical disease stages has led to a more detailed biological characterization of breast cancer. Recently, the attention of the scientific community has focused on the role of tumor-infiltrating lymphocytes (TILs). Therefore, the need of an in-depth immunomorphological characterization of TILs has been emerged. The presence of TILs has been retrospectively investigated in 113 female cases of ductal carcinoma. An immunohistochemical investigation with CD3, CD4, CD8, CD20, CD56, granulysin, perforin-1, granzyme-B and TIA-1 was performed according to the standard procedures on all 17 cases with TILs evidence. TILs consisted of T and B lymphocytes: the prevalent population showed a T immunoprofile with a CD8-immunopositive killer subpopulation (Tk), close-linked to carcinomatous cells, and a CD4-immunopositive helper subpopulation (Th), inside the tumor. A time sequence (firstly T, then B) has been disclosed. Granulysin, perforin, granzyme-B and TIA-1 were expressed by Tk cells. The activated Tk cells secrete these mediators as a result of the binding to the tumor target cell, causing its lytic planned death. The cytotoxicity supported by Tk cells appears an important favorable prognostic factor. Therefore, a graduation system for TILs in breast cancer has been here proposed (absent, non-brisk, brisk).
authors.affiliation: Department of Diagnostic and Clinical Medicine and of Public Health, Section of Pathology, University of Modena and Reggio Emilia, Policlinico Hospital, I-41124, Modena, (MO), Italy. emailmedical@gmail.com.|Provincial Health Care Services, Santa Maria del Carmine Hospital, Institute of Pathology, Rovereto, (TN), Italy. emailmedical@gmail.com.
authors.forename: Luca
authors.initials: L
authors.lastname: Roncati
authors.affiliation: Department of Diagnostic and Clinical Medicine and of Public Health, Section of Pathology, University of Modena and Reggio Emilia, Policlinico Hospital, I-41124, Modena, (MO), Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Barbolini
authors.affiliation: Department of Diagnostic and Clinical Medicine and of Public Health, Section of Oncology, University of Modena and Reggio Emilia, Modena, (MO), Italy.
authors.forename: Federico
authors.initials: F
authors.lastname: Piacentini
authors.affiliation: Provincial Health Care Services, Santa Maria del Carmine Hospital, Institute of Pathology, Rovereto, (TN), Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Piscioli
authors.affiliation: Provincial Health Care Services, Santa Maria del Carmine Hospital, Institute of Pathology, Rovereto, (TN), Italy.
authors.forename: Teresa
authors.initials: T
authors.lastname: Pusiol
authors.affiliation: Department of Diagnostic and Clinical Medicine and of Public Health, Section of Pathology, University of Modena and Reggio Emilia, Policlinico Hospital, I-41124, Modena, (MO), Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Maiorana
mesh_terms: D000328:Adult; D000368:Aged; D000945:Antigens, Differentiation, T-Lymphocyte / Q000378:metabolism; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D001943:Breast Neoplasms / Q000276:immunology* / Q000378:metabolism / Q000473:pathology*; D044584:Carcinoma, Ductal / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D016923:Cell Death / Q000276:immunology; D005260:Female; D053804:Granzymes / Q000378:metabolism; D006801:Humans; D016246:Lymphocytes, Tumor-Infiltrating / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D008875:Middle Aged; D054353:Perforin / Q000378:metabolism; D039102:Poly(A)-Binding Proteins / Q000276:immunology; D011379:Prognosis; D012189:Retrospective Studies; D000074019:T-Cell Intracellular Antigen-1; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism / Q000473:pathology
chemical_list: D000945:Antigens, Differentiation, T-Lymphocyte; C075005:GNLY protein, human; D039102:Poly(A)-Binding Proteins; D000074019:T-Cell Intracellular Antigen-1; C071558:TIA1 protein, human; D054353:Perforin; D053804:Granzymes
keywords: Breast cancer; Granulysin; Histology; Immunohistochemistry; Prognostic factors; Tumor-infiltrating lymphocytes (TILs)