title: Effect of oxidative phosphorylation signaling pathway on silkworm midgut following exposure to phoxim.
abstract: Organophosphate pesticides are applied widely in the world for agricultural purposes, and their exposures often resulted in non-cocooning of Bombyx mori in China. Silkworm midgut is the major organ for digestion and nutrient absorption, importantly it is also a barrier against foreign substances and chemical pesticides. The purpose of this study was to determine the mechanism of oxidative injury in silkworm midgut with phoxim induction. The results showed that the transcription level of oxidative phosphorylation signaling pathway genes of midgut under phoxim stress. Digital gene expression (DGE) analysis revealed that 24 electron transport chain (ETC)-related genes were upregulated. Quantitative real time polymerase chain reaction results indicated that the ETC the genes encoding NADH-CoQ1, Succinic-Q, cyt c reductase-S, cyt c oxidase-S, cytochrome c oxidase polypeptide IV, ATP synthase, and vacuolar H+ ATP synthase were all significantly up-regulated by 1.50-, 1.31-, 1.42-, 1.44-, 1.70-, 2.03- and 1.43-fold, respectively. Phoxim induction enhanced the activity of ETC complex in mitochondria, and induced the accumulation of ROS in midgut. These results indicated that trace phoxim enhanced respiration in midgut, and the imbalance between the activity changes of ETC may led to reactive oxygen species accumulation. The ETC of mitochondria may be potential biomarkers of midgut toxicity in B. mori caused by phoxim exposure. © 2015 Wiley Periodicals, Inc. Environ Toxicol 32: 167-175, 2017.
authors.affiliation: School of Basic Medicine and Biological Sciences, Soochow University, Suzhou, Jiangsu, 215123, People's Republic of China.
authors.forename: Fanchi
authors.initials: F
authors.lastname: Li
authors.affiliation: School of Basic Medicine and Biological Sciences, Soochow University, Suzhou, Jiangsu, 215123, People's Republic of China.
authors.forename: Kaizun
authors.initials: K
authors.lastname: Xu
authors.affiliation: School of Basic Medicine and Biological Sciences, Soochow University, Suzhou, Jiangsu, 215123, People's Republic of China.
authors.forename: Min
authors.initials: M
authors.lastname: Ni
authors.affiliation: School of Basic Medicine and Biological Sciences, Soochow University, Suzhou, Jiangsu, 215123, People's Republic of China.
authors.forename: Binbin
authors.initials: B
authors.lastname: Wang
authors.affiliation: School of Basic Medicine and Biological Sciences, Soochow University, Suzhou, Jiangsu, 215123, People's Republic of China.
authors.forename: Zhiya
authors.initials: Z
authors.lastname: Gu
authors.affiliation: School of Basic Medicine and Biological Sciences, Soochow University, Suzhou, Jiangsu, 215123, People's Republic of China.|National Engineering Laboratory for Modern Silk, Soochow University, Suzhou, Jiangsu, 215123, People's Republic of China.
authors.forename: Weide
authors.initials: W
authors.lastname: Shen
authors.affiliation: School of Basic Medicine and Biological Sciences, Soochow University, Suzhou, Jiangsu, 215123, People's Republic of China.|National Engineering Laboratory for Modern Silk, Soochow University, Suzhou, Jiangsu, 215123, People's Republic of China.
authors.forename: Bing
authors.initials: B
authors.lastname: Li
mesh_terms: D000818:Animals; D015415:Biomarkers; D012831:Bombyx / Q000187:drug effects*; D041981:Gastrointestinal Tract / Q000187:drug effects* / Q000201:enzymology; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D006128:Growth / Q000187:drug effects; D007306:Insecticides / Q000633:toxicity*; D007814:Larva / Q000187:drug effects / Q000254:growth & development; D008928:Mitochondria / Q000187:drug effects / Q000201:enzymology; D009946:Organothiophosphorus Compounds / Q000633:toxicity*; D010085:Oxidative Phosphorylation / Q000187:drug effects*; D018384:Oxidative Stress / Q000187:drug effects; D015398:Signal Transduction / Q000187:drug effects*
chemical_list: D015415:Biomarkers; D007306:Insecticides; D009946:Organothiophosphorus Compounds; C003135:phoxim
keywords: Bombyx mori; ROS; digital gene expression; oxidative phosphorylation; phoxim