title: Ferritin is associated with neural differentiation of bone marrow-derived mesenchymal stem cells under extremely low-frequency electromagnetic field.
abstract: Extremely low-frequency electromagnetic field (ELFEF) is a well-known mechanical stimulation that induces neural differentiation. It is potentially an effective treatment for neurodegenerative diseases. In a previous study, ferritin light chain was upregulated in ELFEF-exposed human bone marrow-derived mesenchymal stem cells (BM-MSCs). Ferritin light chain is a component of ferritin, a highly conserved iron-binding protein. In this study, to identify molecules associated with ferritin during neural differentiation of BM-MSCs, we performed reverse transcription polymerase chain reaction (RT-PCR), western blotting, and ATP analysis. Our data indicated that ELFEF triggers the upregulation of ferritin light chain (FLC) and ferritin heavy chain (FHC) in BM-MSCs. The elevated levels of FLC and FHC correlated positively with the differentiation of BM-MSCs into neural cells. Moreover ELFEF induced the activation of iron regulatory protein-1 (IRP-1) and cofilin, which are downstream targets of ferritin. These results suggest that ELFEF induces neural differentiation through activation of a ferritin-regulated mechanism.
authors.affiliation: Korea University Department of Biotechnology, College of Life Sciences and Biotechnology Seoul Korea.
authors.forename: H-N
authors.initials: HN
authors.lastname: Lee
authors.affiliation: Korea University Department of Biotechnology, College of Life Sciences and Biotechnology Seoul Korea.
authors.forename: K-N
authors.initials: KN
authors.lastname: Ko
authors.affiliation: Korea University Department of Biotechnology, College of Life Sciences and Biotechnology Seoul Korea.
authors.forename: H-J
authors.initials: HJ
authors.lastname: Kim
authors.affiliation: Korea University Department of Biotechnology, College of Life Sciences and Biotechnology Seoul Korea.
authors.forename: A
authors.initials: A
authors.lastname: Rosebud Aikins
authors.affiliation: Korea University Department of Biotechnology, College of Life Sciences and Biotechnology Seoul Korea cwkim@korea.ac.kr.
authors.forename: C-W
authors.initials: CW
authors.lastname: Kim
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D003676:Deferoxamine / Q000494:pharmacology; D004574:Electromagnetic Fields; D005293:Ferritins / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D009474:Neurons / Q000166:cytology / Q000502:physiology
chemical_list: D000255:Adenosine Triphosphate; D005293:Ferritins; D003676:Deferoxamine