title: Three-dimensional direct cell patterning in collagen hydrogels with near-infrared femtosecond laser.
abstract: We report a methodology for three-dimensional (3D) cell patterning in a hydrogel in situ. Gold nanorods within a cell-encapsulating collagen hydrogel absorb a focused near-infrared femtosecond laser beam, locally denaturing the collagen and forming channels, into which cells migrate, proliferate, and align in 3D. Importantly, pattern resolution is tunable based on writing speed and laser power, and high cell viability (>90%) is achieved using higher writing speeds and lower laser intensities. Overall, this patterning technique presents a flexible direct-write method that is applicable in tissue engineering systems where 3D alignment is critical (such as vascular, neural, cardiac, and muscle tissue).
authors.affiliation: Department of NanoEngineering, University of California San Diego, La Jolla, CA, 92093-0448.
authors.forename: Kolin C
authors.initials: KC
authors.lastname: Hribar
authors.affiliation: Department of NanoEngineering, University of California San Diego, La Jolla, CA, 92093-0448.
authors.forename: Kyle
authors.initials: K
authors.lastname: Meggs
authors.affiliation: Materials Science and Engineering Program, University of California San Diego, La Jolla, CA.
authors.forename: Justin
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of NanoEngineering, University of California San Diego, La Jolla, CA, 92093-0448.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhu
authors.affiliation: Department of NanoEngineering, University of California San Diego, La Jolla, CA, 92093-0448.
authors.forename: Xin
authors.initials: X
authors.lastname: Qu
authors.affiliation: Department of NanoEngineering, University of California San Diego, La Jolla, CA, 92093-0448.
authors.forename: Shaochen
authors.initials: S
authors.lastname: Chen
mesh_terms: D000818:Animals; D002460:Cell Line; D002465:Cell Movement / Q000528:radiation effects; D002470:Cell Survival / Q000528:radiation effects; D003094:Collagen / Q000737:chemistry*; D006046:Gold / Q000737:chemistry; D020100:Hydrogels / Q000737:chemistry*; D007834:Lasers; D051379:Mice; D043942:Nanotubes / Q000737:chemistry; D013997:Time Factors
chemical_list: D020100:Hydrogels; D006046:Gold; D003094:Collagen