title: Triggering co-stimulation directly in melanoma tumor fragments drives CD8+tumor-infiltrating lymphocyte expansion with improved effector-memory properties.
abstract: TIL from solid tumors can express activation/co-stimulatory molecules like 4-1BB/CD137, a sign of recent antigenic stimulation in the tumor microenvironment (TME). This activated state can be exploitedex vivoto enhance the expansion of tumor-reactive CD8+TIL for adoptive cell therapy through direct addition of immunomodulators to tumor fragments in culture.
authors.affiliation: Ovarian Cancer Research Center; The University of Pennsylvania ; Philadelphia, PA USA.
authors.forename: Jessica Ann
authors.initials: JA
authors.lastname: Chacon
authors.affiliation: Donald A Adam Comprehensive Melanoma Research Center; H. Lee Moffitt Cancer Center ; Tampa, FL USA.
authors.forename: Amod A
authors.initials: AA
authors.lastname: Sarnaik
authors.affiliation: Donald A Adam Comprehensive Melanoma Research Center; H. Lee Moffitt Cancer Center ; Tampa, FL USA.
authors.forename: Shari
authors.initials: S
authors.lastname: Pilon-Thomas
authors.affiliation: Department of Immunology; H. Lee Moffitt Cancer Center ; Tampa, FL USA ; Lion Biotechnologies ; Tampa, FL USA.
authors.forename: Laszlo
authors.initials: L
authors.lastname: Radvanyi
keywords: 4–1BB; Tumor-Infiltrating Lymphocytes; adoptive T-cell therapy; co-stimulation