title: Positive correlation between patency and mRNA levels for cyclooxygenase-2 and prostaglandin E synthase in the uterine cervix of bitches with pyometra.
abstract: Factors involved in patency of uterine cervices in the bitch with pyometra remain to be clarified. This study examined relationship between patency and mRNA levels for inducible nitric oxide synthase (iNOS), cyclooxygenase (COX)-1, COX-2 and prostaglandin E synthase (PGES) in the uterine cervix of bitches with pyometra. Cervical patency was measured by inserting the stainless steel rods with different diameter into cervical canals. Levels of mRNA expression were determined by semi-quantitative reverse transcription-polymerase chain reaction. The cervical patency was positively correlated with mRNA levels for COX-2 and PGES, but not those for iNOS and COX-1. The results suggest that gene expression of COX-2 and PGES may be involved in the regulation of patency in the uterine cervix of bitches with pyometra.
authors.affiliation: Department of Advanced Pathobiology, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Osaka 598-8531, Japan.
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Tamada
authors.forename: Nahoko
authors.initials: N
authors.lastname: Adachi
authors.forename: Noritoshi
authors.initials: N
authors.lastname: Kawate
authors.forename: Toshio
authors.initials: T
authors.lastname: Inaba
authors.forename: Shingo
authors.initials: S
authors.lastname: Hatoya
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Sawada
mesh_terms: D000818:Animals; D002584:Cervix Uteri / Q000201:enzymology* / Q000503:physiopathology; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism*; D004283:Dog Diseases / Q000201:enzymology* / Q000235:genetics; D004285:Dogs; D005260:Female; D015870:Gene Expression; D052247:Nitric Oxide Synthase Type II / Q000235:genetics / Q000378:metabolism; D000071876:Prostaglandin-E Synthases / Q000235:genetics / Q000378:metabolism*; D055112:Pyometra / Q000201:enzymology / Q000235:genetics / Q000503:physiopathology / Q000662:veterinary*; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D052247:Nitric Oxide Synthase Type II; D051546:Cyclooxygenase 2; D000071876:Prostaglandin-E Synthases