title: Single-cell sequencing in cancer research.
abstract: Genome-wide single-cell sequencing investigations have the potential to classify individual cells within a tumor mass. In recent years, various single-cell DNA and RNA quantification techniques have facilitated significant advances in our ability to classify subpopulations of cells within a heterogeneous population. These approaches provide the possibility of unraveling the complex variability in genetic, epigenetic and transcriptional interactions that occur within identical cells in a tumor. This should enhance our knowledge of the underlying biological phenotypes and could have a huge impact in designing more precise anticancer treatments in order to improve outcomes and avoid tumor resistance. In addition, single-cell sequencing analysis has the potential to allow the development of better diagnostic and prognostic biomarkers, and thus aid the delivery of more personalized targeted cancer therapy. Nevertheless, further research is still required to overcome technical, biological and computational problems before clinical application.
authors.affiliation: a Division of Cancer, Department of Surgery & Cancer , Imperial Centre for Translational and Experimental Medicine (ICTEM), Imperial College , Hammersmith Hospital campus, Du Cane Road, London , W12 0NN , UK.
authors.forename: Mireia
authors.initials: M
authors.lastname: Mato Prado
authors.affiliation: a Division of Cancer, Department of Surgery & Cancer , Imperial Centre for Translational and Experimental Medicine (ICTEM), Imperial College , Hammersmith Hospital campus, Du Cane Road, London , W12 0NN , UK.|b HPB Surgical Unit, Department of Surgery & Cancer , Imperial College , Hammersmith Hospital campus, Du Cane Road, London , W12 0HS , UK.
authors.forename: Adam E
authors.initials: AE
authors.lastname: Frampton
authors.affiliation: a Division of Cancer, Department of Surgery & Cancer , Imperial Centre for Translational and Experimental Medicine (ICTEM), Imperial College , Hammersmith Hospital campus, Du Cane Road, London , W12 0NN , UK.
authors.forename: Justin
authors.initials: J
authors.lastname: Stebbing
authors.forename: Jonathan
authors.initials: J
authors.lastname: Krell
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006801:Humans; D009369:Neoplasms / Q000235:genetics*; D057089:Pathology, Molecular; D059010:Single-Cell Analysis / Q000379:methods*
chemical_list: D014408:Biomarkers, Tumor
keywords: biomarkers; cancer; diagnosis; prognosis; single-cell sequencing