title: Synthesis of silver nanoparticles on the basis of low and high molar mass exopolysaccharides of Bradyrhizobium japonicum 36 and its antimicrobial activity against some pathogens.
abstract: Silver nanoparticles (SNPs) were synthesized on the basis of exopolysaccharides (low and high molar mass) of diazotrophic Bradyrhizobium japonicum 36 strain. The synthesis of SNPs was carried out by direct reduction of silver nitrate with ethanol-insoluble (high molar mass, HMW) and ethanol-soluble (low molar mass, LMW) fractions of exopolysaccharides (EPS), produced by diazotrophic strain B. japonicum 36. SNPs were characterized using UV-vis spectroscopy, transmission electron microscopy (TEM), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR). SNPs synthesized on the basis of LMW EPS absorbed radiation in the visible regions of 420 nm, whereas SNPs based on the HMW EPS have a wavelength maximum at 450 nm because of the strong SPR transition. Moreover, the antibacterial and antifungal activities of the SNPs were examined in vitro against Escherichia coli, Staphylococcus aureus, and Candida albicans. SNPs synthesized on the basis of LMW EPS were active than those synthesized on the basis of HMW EPS. Besides, UV-visible spectroscopic evaluation confirmed that SNPs synthesized on the basis of LMW EPS were far more stable than those obtained on the basis of HMW EPS.
authors.affiliation: Institute of Genetics and Plant Experimental Biology, Uzbekistan Academy of Sciences, Kibray District, Yukori Yuz, 111126, Tashkent, Uzbekistan.|Key Laboratory of Plant Resources and Chemistry in Arid Regions, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, South Beijing Road 40-1, Urumqi, Xinjiang, 830011, People's Republic of China.
authors.forename: Bakhtiyor
authors.initials: B
authors.lastname: Rasulov
authors.affiliation: Key Laboratory of Plant Resources and Chemistry in Arid Regions, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, South Beijing Road 40-1, Urumqi, Xinjiang, 830011, People's Republic of China.|Department of Biology, Samarkand State University, University Boulevard 15, Samarkand, 703004, Uzbekistan.
authors.forename: Nigora
authors.initials: N
authors.lastname: Rustamova
authors.affiliation: Key Laboratory of Plant Resources and Chemistry in Arid Regions, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, South Beijing Road 40-1, Urumqi, Xinjiang, 830011, People's Republic of China.
authors.forename: Abulimiti
authors.initials: A
authors.lastname: Yili
authors.affiliation: Key Laboratory of Plant Resources and Chemistry in Arid Regions, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, South Beijing Road 40-1, Urumqi, Xinjiang, 830011, People's Republic of China.
authors.forename: Hai-Qing
authors.initials: HQ
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Plant Resources and Chemistry in Arid Regions, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, South Beijing Road 40-1, Urumqi, Xinjiang, 830011, People's Republic of China. haji@ms.xjb.ac.cn.
authors.forename: Haji A
authors.initials: HA
authors.lastname: Aisa
mesh_terms: D000890:Anti-Infective Agents / Q000378:metabolism* / Q000494:pharmacology*; D020369:Bradyrhizobium / Q000378:metabolism*; D002176:Candida albicans / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D010084:Oxidation-Reduction; D011135:Polysaccharides, Bacterial / Q000378:metabolism; D012834:Silver / Q000378:metabolism* / Q000494:pharmacology*; D013053:Spectrophotometry; D017550:Spectroscopy, Fourier Transform Infrared; D013211:Staphylococcus aureus; D014961:X-Ray Diffraction
chemical_list: D000890:Anti-Infective Agents; D011135:Polysaccharides, Bacterial; D012834:Silver