title: The dual aptamer approach: rational design of a high-affinity FAD aptamer.
abstract: A design strategy for high-affinity aptamers of complex biomolecules is presented. We developed an RNA with FAD-binding properties by combining known ATP- and FMN-aptamers. Cooperative binding of FAD was shown by SPR spectroscopy and fluorescence assays. The strategy should be transferable to several other biomolecules.
authors.affiliation: Department of Chemistry and Konstanz Research School Chemical Biology (KoRS-CB), University of Konstanz, Universitätsstr. 10, 78457 Konstanz, Germany. joerg.hartig@uni-konstanz.de.
authors.forename: T
authors.initials: T
authors.lastname: Merkle
authors.forename: I T
authors.initials: IT
authors.lastname: Holder
authors.forename: J S
authors.initials: JS
authors.lastname: Hartig
mesh_terms: D000255:Adenosine Triphosphate / Q000737:chemistry; D052157:Aptamers, Nucleotide / Q000138:chemical synthesis* / Q000737:chemistry*; D015195:Drug Design; D005486:Flavin Mononucleotide / Q000737:chemistry; D005182:Flavin-Adenine Dinucleotide / Q000737:chemistry*; D015394:Molecular Structure; D012313:RNA / Q000138:chemical synthesis / Q000737:chemistry*
chemical_list: D052157:Aptamers, Nucleotide; D005182:Flavin-Adenine Dinucleotide; D012313:RNA; D005486:Flavin Mononucleotide; D000255:Adenosine Triphosphate