title: Outline of a bacterial filter-based assay to detect beta-lactamases.
abstract: We describe a new phenotypic test to detect beta-lactamases. This assay is based on diffusion of beta-lactam/beta-lactamase through a bacterial filter. Beta-lactam hydrolysis on (the other side of) the filter leads to a change in antibiotic susceptibility, which can be measured by disc diffusion tests. We illustrate its ease of use to detect beta-lactamases of different classes.
authors.affiliation: Department of Medical Microbiology and Infection Control, VU University Medical Center, Amsterdam, The Netherlands.
authors.forename: B B
authors.initials: BB
authors.lastname: Wintermans
authors.affiliation: Department of Medical Microbiology and Infection Control, VU University Medical Center, Amsterdam, The Netherlands.
authors.forename: C M J E
authors.initials: CM
authors.lastname: Vandenbroucke-Grauls
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D001426:Bacterial Proteins / Q000032:analysis / Q000737:chemistry*; D001431:Bacteriological Techniques; D052978:Disk Diffusion Antimicrobial Tests; D008826:Microbial Sensitivity Tests / Q000379:methods; D010641:Phenotype; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000032:analysis / Q000737:chemistry*; D047090:beta-Lactams / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D047090:beta-Lactams; D001618:beta-Lactamases; C063912:carbapenemase
keywords: Beta-lactamase; Carbapenemase; ESBL; Phenotypic method; Resistance