title: Glutathione S-transferase pi (GST-pi) inhibition and anti-inflammation activity of the ethyl acetate extract of Streptomyces sp. strain MJM 8637.
abstract: To investigate the anti-cancer properties of soil-borne actinobacteria, MJM 8637, the glutathione S-transferase pi (GST-pi) assay, anti-tumor necrosis factor (TNF)-α assay, the level of antioxidant potential by DPPH radical scavenging activity, NO scavenging activity, and ABTS radical scavenging activity in ethyl acetate extract were determined. The 16S rDNA sequencing analysis revealed that Streptomyces sp. strain MJM 8637, which was isolated from Hambak Mountain, Korea, has 99.5% similarity to Streptomyces atratus strain NBRC 3897. The physiological and the morphological characteristics of the strain MJM 8637 were also identified. The ethyl acetate extract of MJM 8637 inhibited TNF-α production approximately 61.8% at concentration 100 μg/ml. The IC50 value of the strain MJM 8637 extract on GST-pi was identified to be 120.2 ± 1.6 μg/ml. In DPPH, NO, and ABTS radical scavenging assays, the IC50 values of the strain MJM 8637 extract were found to be 977.2 μg/ml, 1143.7 μg/ml, and 454.4 μg/ml, respectively. The ethyl acetate extract of the strain MJM 8637 showed 97.2 ± 1.3% of cell viability at 100 μg/ml in RAW 264.7 cell viability assay. The results obtained from this study suggest that the ethyl acetate extract of Streptomyces sp. strain MJM 8637 could be considered as a potential source of drug for the cancers that have multidrug resistance with its GST-pi inhibition and anti-inflammation activities, and low cytotoxicity.
authors.affiliation: Center for Neutraceutical and Pharmaceutical Materials, Myongji University, Yongin, Republic of Korea.
authors.forename: Sung-Kwon
authors.initials: SK
authors.lastname: Lee
authors.affiliation: Center for Neutraceutical and Pharmaceutical Materials, Myongji University, Yongin, Republic of Korea ; Division of Bioscience and Bioinformatics, Myongji University, Yongin, Republic of Korea.
authors.forename: Dong-Ryung
authors.initials: DR
authors.lastname: Lee
authors.affiliation: Center for Neutraceutical and Pharmaceutical Materials, Myongji University, Yongin, Republic of Korea.
authors.forename: Bong-Keun
authors.initials: BK
authors.lastname: Choi
authors.affiliation: Center for Neutraceutical and Pharmaceutical Materials, Myongji University, Yongin, Republic of Korea ; Interdisciplinary Program of Biomodulation, Myongji University, Yongin, Republic of Korea.
authors.forename: Sasikumar Arunachalam
authors.initials: SA
authors.lastname: Palaniyandi
authors.affiliation: Center for Neutraceutical and Pharmaceutical Materials, Myongji University, Yongin, Republic of Korea ; Interdisciplinary Program of Biomodulation, Myongji University, Yongin, Republic of Korea.
authors.forename: Seung Hwan
authors.initials: SH
authors.lastname: Yang
authors.affiliation: Center for Neutraceutical and Pharmaceutical Materials, Myongji University, Yongin, Republic of Korea ; Division of Bioscience and Bioinformatics, Myongji University, Yongin, Republic of Korea.
authors.forename: Joo-Won
authors.initials: JW
authors.lastname: Suh
keywords: Anti-inflammation activity; GST-pi inhibition activity; Streptomyces sp. strain MJM 8637