title: Suppression of OsKu80 results in defects in developmental growth and increased telomere length in rice (Oryza sativa L.).
abstract: The Ku70-Ku80 heterodimer plays a critical role in the maintenance of genomic stability in humans and yeasts. In this report, we identified and characterized OsKu80 in rice, a model monocot crop. OsKu80 forms a heterodimer with OsKu70 in yeast and plant cells, as demonstrated by yeast two-hybrid, in vivo co-immunoprecipitation, and bimolecular fluorescence complementation assays. RNAi-mediated knock-down T3 transgenic rice plants (Ubi:RNAi-OsKu80) displayed a retarded growth phenotype at the post-germination stage. In addition, the Ubi:RNAi-OsKu80 knock-down progeny exhibited noticeably increased telomere length as compared to wild-type rice. These results are discussed with the idea that OsKu80 plays a role in developmental growth and telomere length regulation in rice plants.
authors.affiliation: Department of Systems Biology, College of Life Science and Biotechnology, Yonsei University, Seoul 120-749, South Korea.
authors.forename: Mi Young
authors.initials: MY
authors.lastname: Byun
authors.affiliation: Department of Systems Biology, College of Life Science and Biotechnology, Yonsei University, Seoul 120-749, South Korea.
authors.forename: Li Hua
authors.initials: LH
authors.lastname: Cui
authors.affiliation: Department of Systems Biology, College of Life Science and Biotechnology, Yonsei University, Seoul 120-749, South Korea. Electronic address: wtkim@yonsei.ac.kr.
authors.forename: Woo Taek
authors.initials: WT
authors.lastname: Kim
mesh_terms: D029681:Arabidopsis Proteins / Q000235:genetics*; D004265:DNA Helicases / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D042822:Genomic Instability / Q000502:physiology; D012275:Oryza / Q000235:genetics* / Q000254:growth & development*; D030821:Plants, Genetically Modified / Q000502:physiology; D059505:Telomere Homeostasis / Q000235:genetics*
chemical_list: D029681:Arabidopsis Proteins; D004265:DNA Helicases; C469228:Ku80 protein, Arabidopsis
keywords: Developmental growth; Genome stability; Ku70/Ku80 heterodimer; Ku80; Rice (Oryza sativa L.); Telomere