title: Structural insights into YfiR sequestering by YfiB in Pseudomonas aeruginosa PAO1.
abstract: YfiBNR is a tripartite signalling system in Pseudomonas aeruginosa that modulates intracellular c-di-GMP levels in response to signals received in the periplasm. YfiB is an outer membrane lipoprotein and presumed sensor protein that sequesters the repressor protein YfiR. To provide insights into YfiBNR function, we have determined three-dimensional crystal structures of YfiB and YfiR from P. aeruginosa PAO1 alone and as a 1:1 complex. A YfiB(27-168) construct is predominantly dimeric, whereas a YfiB(59-168) is monomeric, indicating that YfiB can dimerize via its N-terminal region. YfiR forms a stable complex with YfiB(59-168), while the YfiR binding interface is obstructed by the N-terminal region in YfiB(27-168). The YfiB-YfiR complex reveals a conserved interaction surface on YfiR that overlaps with residues predicted to interact with the periplasmic PAS domain of YfiN. Comparison of native and YfiR-bound structures of YfiB suggests unwinding of the N-terminal linker region for attachment to the outer membrane. A model is thus proposed for YfiR sequestration at the outer membrane by YfiB. Our work provides the first detailed insights into the interaction between YfiB and YfiR at the molecular level and is a valuable starting point for further functional and mechanistic studies of the YfiBNR signalling system.
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin, China.|College of Life Sciences, Nankai University, Tianjin, China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Li
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin, China.|College of Life Sciences, Nankai University, Tianjin, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Li
authors.affiliation: College of Life Sciences, Nankai University, Tianjin, China.
authors.forename: Yueyang
authors.initials: Y
authors.lastname: Xu
authors.affiliation: College of Life Sciences, Nankai University, Tianjin, China.
authors.forename: Qionglin
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: College of Life Sciences, Nankai University, Tianjin, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin, China.|College of Life Sciences, Nankai University, Tianjin, China.
authors.forename: Shiyou
authors.initials: S
authors.lastname: Che
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin, China.|College of Life Sciences, Nankai University, Tianjin, China.
authors.forename: Ruihua
authors.initials: R
authors.lastname: Liu
authors.affiliation: Key Laboratory of Pollution Processes and Environmental Criteria (Ministry of Education), College of Environmental Science &Engineering, Nankai University, Tianjin, China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin, China.|College of Life Sciences, Nankai University, Tianjin, China.
authors.forename: Mark
authors.initials: M
authors.lastname: Bartlam
mesh_terms: D020816:Amino Acid Motifs; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D001665:Binding Sites; D003001:Cloning, Molecular; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D015964:Gene Expression Regulation, Bacterial; D057927:Hydrophobic and Hydrophilic Interactions; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009154:Mutation; D019897:Periplasm / Q000737:chemistry / Q000378:metabolism*; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding; D055503:Protein Multimerization; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011550:Pseudomonas aeruginosa / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D001426:Bacterial Proteins; D011994:Recombinant Proteins