title: Fungal Community Assembly in the Amazonian Dark Earth.
abstract: Here, we compare the fungal community composition and diversity in Amazonian Dark Earth (ADE) and the respective non-anthropogenic origin adjacent (ADJ) soils from four different sites in Brazilian Central Amazon using pyrosequencing of 18S ribosomal RNA (rRNA) gene. Fungal community composition in ADE soils were more similar to each other than their ADJ soils, except for only one site. Phosphorus and aluminum saturation were the main soil chemical factors contributing to ADE and ADJ fungal community dissimilarities. Differences in fungal richness were not observed between ADE and ADJ soil pairs regarding to the most sites. In general, the most dominant subphyla present in the soils were Pezizomycotina, Agaricomycotina, and Mortierellomycotina. The most abundant operational taxonomic units (OTUs) in ADE showed similarities with the entomopathogenic fungus Cordyceps confragosa and the saprobes Fomitopsis pinicola, Acremonium vitellinum, and Mortierellaceae sp., whereas OTUs similar to Aspergillus niger, Lithothelium septemseptatum, Heliocephala gracillis, and Pestalosphaeria sp. were more abundant in ADJ soils. Differences in fungal community composition were associated to soil chemical factors in ADE (P, Ca, Zn, Mg, organic matter, sum of bases, and base saturation) and ADJ (Al, potential acidity, Al saturation, B, and Fe) soils. These results contribute to a deeper view of the fungi communities in ADE and open new perspectives for entomopathogenic fungi studies.
authors.affiliation: Department of Microbial Ecology, Netherlands Institute of Ecology (NIOO/KNAW), Droevendaalsesteeg 10, Wageningen, 6708 PB, The Netherlands.
authors.forename: Adriano Reis
authors.initials: AR
authors.lastname: Lucheta
authors.affiliation: Centro de Energia Nuclear na Agricultura (CENA), Universidade de São Paulo (USP), Piracicaba, Brazil.
authors.forename: Fabiana de Souza
authors.initials: Fde S
authors.lastname: Cannavan
authors.affiliation: Centro Interdisciplinar de Pesquisas em Biotecnologia (CIP-Biotec), Universidade Federal do Pampa, São Gabriel, Brazil.
authors.forename: Luiz Fernando Wurdig
authors.initials: LF
authors.lastname: Roesch
authors.affiliation: Centro de Energia Nuclear na Agricultura (CENA), Universidade de São Paulo (USP), Piracicaba, Brazil.
authors.forename: Siu Mui
authors.initials: SM
authors.lastname: Tsai
authors.affiliation: Department of Microbial Ecology, Netherlands Institute of Ecology (NIOO/KNAW), Droevendaalsesteeg 10, Wageningen, 6708 PB, The Netherlands. e.kuramae@nioo.knaw.nl.
authors.forename: Eiko Eurya
authors.initials: EE
authors.lastname: Kuramae
mesh_terms: D044822:Biodiversity; D001938:Brazil; D004463:Ecology; D005658:Fungi / Q000145:classification / Q000235:genetics / Q000254:growth & development*; D010802:Phylogeny; D012337:RNA, Ribosomal, 18S; D017422:Sequence Analysis, DNA; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology
chemical_list: D012337:RNA, Ribosomal, 18S; D012987:Soil
keywords: 18S rRNA; Anthrosols; Biochar; Microbial ecology; Pre-Columbian soil; Pyrosequencing