title: [Transglycosylation of neolignans by enzymatic synthesis and evaluation of their antitumor activity].
abstract: OBJECTIVE
  To improve the water solubility and biological activity of neoligans (magnolol and honokiol) and test the antitumor activity of the modified compounds.
  
  
  METHODS
  The glycosylated products of magnolol and honokiol were obtained by enzymatic synthesis using a UDP-glycosyltransferase (YjiC) from Bacillus. The products were characterized by high-performance liquid chromatography (HPLC), liquid chromatography-mass spectrometry (LC-MS), and nuclear magnetic resonance (NMR) analysis. MTT assay was used to detect the growth inhibition of 4 human cancer cell lines induced by the compounds.
  
  
  RESULTS
  We obtained two glucosides of neolignans (magnolol and honokiol) for the first time by enzymatic synthesis using a UDP-glycosyltransferase. Based on the spectroscopic data, the glucosides were identified as magnolol-2- O-β-D-glucopyranoside (1) and honokiol-4'-O-β-D-glucopyranoside (2). Compounds 1-4 exhibited moderate anti-proliferative activities against the 4 human cancer cell lines, with IC50 values ranging from 9.41 to 111.21 µmol/L.
  
  
  CONCLUSIONS
  The glycoslated products show enhanced water solubility and drug sensitivity against SMMC7721 cells, suggesting their value as potential therapeutic drugs.
authors.affiliation: Faculty of Pharmacy, Bengbu Medical College, Bengbu 233030, China.E-mail: athongmei @foxmail.com.
authors.forename: Hongmei
authors.initials: H
authors.lastname: Li
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Wei
authors.initials: W
authors.lastname: Jin
authors.forename: Xing
authors.initials: X
authors.lastname: Guo
authors.forename: Nan
authors.initials: N
authors.lastname: Li
authors.forename: Tao
authors.initials: T
authors.lastname: Ma
authors.forename: Qiang
authors.initials: Q
authors.lastname: Huo
authors.forename: ChengZhu
authors.initials: C
authors.lastname: Wu
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry*; D001713:Biphenyl Compounds / Q000737:chemistry*; D045744:Cell Line, Tumor; D002851:Chromatography, High Pressure Liquid; D005960:Glucosides / Q000737:chemistry; D006031:Glycosylation; D006801:Humans; D017705:Lignans / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D000498:Allyl Compounds; D010636:Phenols
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D001713:Biphenyl Compounds; D005960:Glucosides; D017705:Lignans; C005498:magnolol; C005499:honokiol; D000498:Allyl Compounds; D010636:Phenols