title: MAE4, an eLtaS monoclonal antibody, blocks Staphylococcus aureus virulence.
abstract: Staphylococcus aureus causes a wide range of infectious diseases. Treatment of these infections has become increasingly difficult due to the widespread emergence of antibiotic-resistant strains; therefore, it is essential to explore effective alternatives to antibiotics. A secreted protein of S. aureus, known as eLtaS, is an extracellular protein released from the bacterial membrane protein, LtaS. However, the role of eLtaS in S. aureus pathogenesis remains largely unknown. Here we show eLtaS dramatically aggravates S. aureus infection by binding to C3b and then inhibiting the phagocytosis of C3b-deposited S. aureus. Furthermore, we developed a monoclonal antibody against eLtaS, MAE4, which neutralizes the activity of eLtaS and blocks staphylococcal evasion of phagocytosis. Consequently, MAE4 is capable of protecting mice from lethal S. aureus infection. Our findings reveal that targeting of eLtaS by MAE4 is a potential therapeutic strategy for the treatment of infectious diseases caused by S. aureus.
authors.affiliation: Beijing Institute of Basic Medical Sciences, Beijing, China.|State key Laboratory of Toxicology and Medical Countermeasures, Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Beijing Institute of Basic Medical Sciences, Beijing, China.|State key Laboratory of Toxicology and Medical Countermeasures, Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China.
authors.forename: Jiannan
authors.initials: J
authors.lastname: Feng
authors.affiliation: Beijing Institute of Basic Medical Sciences, Beijing, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Lu
authors.affiliation: Institutes of Biomedical Sciences, Fudan University, Shanghai, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Beijing Institute of Basic Medical Sciences, Beijing, China.|State key Laboratory of Toxicology and Medical Countermeasures, Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China.
authors.forename: Yaping
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Beijing Institute of Basic Medical Sciences, Beijing, China.|State key Laboratory of Toxicology and Medical Countermeasures, Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Yan
authors.affiliation: Beijing Institute of Basic Medical Sciences, Beijing, China.
authors.forename: Chunhua
authors.initials: C
authors.lastname: Mu
authors.affiliation: People's Armed Police Corps General Hospital, Beijing, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Hei
authors.affiliation: Beijing Institute of Basic Medical Sciences, Beijing, China.|State key Laboratory of Toxicology and Medical Countermeasures, Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Lv
authors.affiliation: Beijing Institute of Basic Medical Sciences, Beijing, China.|State key Laboratory of Toxicology and Medical Countermeasures, Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China.
authors.forename: Gencheng
authors.initials: G
authors.lastname: Han
authors.affiliation: Beijing Institute of Basic Medical Sciences, Beijing, China.|State key Laboratory of Toxicology and Medical Countermeasures, Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China.
authors.forename: Guojiang
authors.initials: G
authors.lastname: Chen
authors.affiliation: Beijing Institute of Basic Medical Sciences, Beijing, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Jin
authors.affiliation: Institutes of Biomedical Sciences, Fudan University, Shanghai, China.
authors.forename: Weiguo
authors.initials: W
authors.lastname: Hu
authors.affiliation: Beijing Institute of Basic Medical Sciences, Beijing, China.|State key Laboratory of Toxicology and Medical Countermeasures, Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China.
authors.forename: Beifen
authors.initials: B
authors.lastname: Shen
authors.affiliation: Beijing Institute of Basic Medical Sciences, Beijing, China.|State key Laboratory of Toxicology and Medical Countermeasures, Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China.
authors.forename: Guang
authors.initials: G
authors.lastname: Yang
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000494:pharmacology / Q000627:therapeutic use; D001426:Bacterial Proteins / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D003179:Complement C3b / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009504:Neutrophils / Q000166:cytology / Q000276:immunology; D010587:Phagocytosis / Q000187:drug effects; D011485:Protein Binding; D013203:Staphylococcal Infections / Q000188:drug therapy / Q000401:mortality / Q000662:veterinary; D013211:Staphylococcus aureus / Q000378:metabolism / Q000472:pathogenicity*; D015996:Survival Rate; D014774:Virulence / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; D003179:Complement C3b