title: Comparison of the Effects of the Selective Estrogen Receptor Modulators Ospemifene, Raloxifene, and Tamoxifen on Breast Tissue in Ex Vivo Culture.
abstract: Explant tissue culture provides a model for studying the direct effects of steroid hormones, their analogs, and novel hormonally active compounds on normal freshly isolated human breast tissues (HBTs). For this purpose, pre- and postmenopausal HBTs can be maintained in this culture system. The results demonstrate that the morphological integrity of HBT explants can be maintained in tissue culture up to 2 weeks and expression of differentiation markers, steroid hormone receptors, proliferation and apoptosis ratios can be evaluated as a response to hormonal stimulation. This chapter describes an ex vivo culture model that we have applied to study the effects of various hormonally active substances, including 17β-estradiol and selective estrogen receptor modulators (SERMs), on normal human breast tissues.
authors.affiliation: Department of Cell Biology and Anatomy, Institute of Biomedicine, University of Turku, Kiinamyllynkatu 10, Turku, 20520, Finland. nateig@utu.fi.
authors.forename: Natalija
authors.initials: N
authors.lastname: Eigeliene
authors.affiliation: Department of Obstetrics and Gynecology, Turku University Central Hospital, 20520, Turku, Finland.
authors.forename: Risto
authors.initials: R
authors.lastname: Erkkola
authors.affiliation: Department of Cell Biology and Anatomy, Institute of Biomedicine, University of Turku, Kiinamyllynkatu 10, Turku, 20520, Finland.
authors.forename: Pirkko
authors.initials: P
authors.lastname: Härkönen
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D015415:Biomarkers / Q000378:metabolism; D001940:Breast / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology / Q000601:surgery; D049109:Cell Proliferation / Q000187:drug effects; D004958:Estradiol / Q000494:pharmacology; D047628:Estrogen Receptor alpha / Q000187:drug effects* / Q000378:metabolism; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D016612:Paraffin Embedding; D020849:Raloxifene Hydrochloride / Q000494:pharmacology*; D020845:Selective Estrogen Receptor Modulators / Q000494:pharmacology*; D013629:Tamoxifen / Q000031:analogs & derivatives* / Q000494:pharmacology*; D013997:Time Factors; D046509:Tissue Culture Techniques; D016707:Tissue Fixation
chemical_list: D015415:Biomarkers; C506487:ESR1 protein, human; D047628:Estrogen Receptor alpha; D020845:Selective Estrogen Receptor Modulators; D013629:Tamoxifen; D020849:Raloxifene Hydrochloride; D004958:Estradiol; C119141:Ospemifene
keywords: Estradiol; Estrogen receptor alpha; Explant tissue culture; Human breast tissue; SERMs