title: Comparative immune responses against Psoroptes ovis in two cattle breeds with different susceptibility to mange.
abstract: The sheep scab mite, Psoroptes ovis, is a major problem in the beef cattle industry, especially in Belgian Blue (BB) cattle. This breed is naturally more predisposed to psoroptic mange but reasons for this high susceptibility remain unknown. Different immune responses could be a potential cause; thus in this study, the cutaneous immune response and in vitro cellular immune response after antigen re-stimulation were examined in naturally infested BB. Cytokine production in the skin and in circulating re-stimulated peripheral blood mononuclear cells (PBMC) demonstrated a mixed pro-inflammatory Th2/Th17 profile, with transcription of IL-4, IL-13, IL-6 and IL-17. Strong IL-17 up-regulation in the skin of BB was associated with an influx of eosinophils and other immune cells, potentially leading towards more severe symptoms. Virtually no changes in cutaneous IFN-γ transcription were detected, while there was substantial IFN-γ up-regulation in re-stimulated PBMC from infested and uninfested animals, potentially indicating a role of this pro-inflammatory cytokine in the innate immune response. In Holstein-Friesian (HF) cattle, generally more resistant to P. ovis infection, a largely similar immunologic response was observed. Differences between HF and BB were the lack of cutaneous IL-17 response in infested HF and low transcription levels of IFN-γ and high IL-10 transcription in re-stimulated PBMC from both infested and uninfested animals. Further research is needed to identify potential cell sources and biological functions for these cytokines and to fully unravel the basis of this different breed susceptibility to P. ovis.
authors.affiliation: Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium. charlotte.sarre@ugent.be.
authors.forename: Charlotte
authors.initials: C
authors.lastname: Sarre
authors.affiliation: Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium. ana.gonzalez@ugent.be.
authors.forename: Ana
authors.initials: A
authors.lastname: González-Hernández
authors.affiliation: Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium. Stefanie@geka.be.
authors.forename: Stefanie
authors.initials: S
authors.lastname: Van Coppernolle
authors.affiliation: Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium. ggrit@its.jnj.com.
authors.forename: Rika
authors.initials: R
authors.lastname: Grit
authors.affiliation: Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium. korneel.grauwet@ugent.be.
authors.forename: Korneel
authors.initials: K
authors.lastname: Grauwet
authors.affiliation: Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium. frederik.vanmeulder@ugent.be.
authors.forename: Frederik
authors.initials: F
authors.lastname: Van Meulder
authors.affiliation: Department of Pathology, Bacteriology and Poultry Diseases, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium. koen.chiers@ugent.be.
authors.forename: Koen
authors.initials: K
authors.lastname: Chiers
authors.affiliation: Department of Morphology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium. wim.vandenbroeck@ugent.be.
authors.forename: Wim
authors.initials: W
authors.lastname: Van den Broeck
authors.affiliation: Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium. peter.geldhof@ugent.be.
authors.forename: Peter
authors.initials: P
authors.lastname: Geldhof
authors.affiliation: Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium. edwin.claerebout@ugent.be.
authors.forename: Edwin
authors.initials: E
authors.lastname: Claerebout
mesh_terms: D000818:Animals; D001530:Belgium; D001947:Breeding; D002417:Cattle; D002418:Cattle Diseases / Q000235:genetics / Q000276:immunology* / Q000469:parasitology; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D004198:Disease Susceptibility / Q000276:immunology / Q000469:parasitology / Q000662:veterinary*; D005260:Female; D007111:Immunity, Cellular; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008924:Mite Infestations / Q000235:genetics / Q000276:immunology / Q000469:parasitology / Q000662:veterinary*; D040042:Psoroptidae / Q000502:physiology*; D015854:Up-Regulation
chemical_list: D016207:Cytokines