title: Production of a bioactive sweetener steviolbioside via specific hydrolyzing ester linkage of stevioside with a β-galactosidase.
abstract: A β-galactosidase from Kluyveromyces lactis was found to specifically catalyze hydrolysis of the glycosyl ester linkage of stevioside to yield steviolbioside, a rare sweetener that also exists in Stevia rebaudiana leaves. In a packed bed reactor, a reaction coupling separation was realized and a production yield of steviolbioside reached 90% in 6 h. The hydrolysis product steviolbioside presented higher cytoxicity on human normal cells (hepatocytes cell L02 and intestinal epithelial cell T84) than stevioside did. Comparing to the typical chemotherapy agent, 5-fluorouracil (5-FU), steviolbioside presents much lower cytotoxicity on all assayed human normal cells; it presented notable inhibition on human hepatocarcinoma cell Hep3B, human breast cancer cell MDA-MB-231 and human pancreatic cancer cell BxPC-3. The remarkable inhibition on MDA-MB-231 cells makes steviolbioside a potential remedy for human breast cancer, when steviolbioside is served as a natural sweetener.
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Chemical and Materials Engineering, Jiangnan University, Wuxi, Jiangsu 214122, China.
authors.forename: Jun-ming
authors.initials: JM
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Chemical and Materials Engineering, Jiangnan University, Wuxi, Jiangsu 214122, China.
authors.forename: Li
authors.initials: L
authors.lastname: Ding
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Chemical and Materials Engineering, Jiangnan University, Wuxi, Jiangsu 214122, China.
authors.forename: Xiao-chen
authors.initials: XC
authors.lastname: Sui
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Chemical and Materials Engineering, Jiangnan University, Wuxi, Jiangsu 214122, China. Electronic address: ymxia@jiangnan.edu.cn.
authors.forename: Yong-mei
authors.initials: YM
authors.lastname: Xia
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Chemical and Materials Engineering, Jiangnan University, Wuxi, Jiangsu 214122, China.
authors.forename: Hui-da
authors.initials: HD
authors.lastname: Wan
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Chemical and Materials Engineering, Jiangnan University, Wuxi, Jiangsu 214122, China.
authors.forename: Tong
authors.initials: T
authors.lastname: Lu
mesh_terms: D045786:Diterpenes, Kaurane / Q000737:chemistry*; D005960:Glucosides / Q000737:chemistry*; D006801:Humans; D013549:Sweetening Agents / Q000737:chemistry*; D001616:beta-Galactosidase / Q000737:chemistry*
chemical_list: D045786:Diterpenes, Kaurane; D005960:Glucosides; D013549:Sweetening Agents; C074471:steviolbioside; C012043:stevioside; D001616:beta-Galactosidase
keywords: Anti-cancer; Hydrolysis; Reaction coupling separation; Steviolbioside; Stevioside; β-Galactosidase