title: Antidiabetic and antihyperlipidemic activities of a novel polyherbal formulation in high fat diet/streptozotocin induced diabetic rat model.
abstract: OBJECTIVE
  To investigate the antidiabetic and antihyperlipidemic activities of polyherbal formulation (PHF) containing hydroalcoholic extracts of four plants namely Salacia oblonga, Salacia roxbhurgii, Garcinia indica and Lagerstroemia parviflora in streptozotocin (STZ)-induced diabetic rats by administering oral doses (200 and 400 mg/kg body weight).
  
  
  METHODS
  Animals were divided into diabetic and nondiabetic groups. Rats were fed with a high-fat diet (HFD) and induced with a single low dose of STZ (35 mg/kg) i.p. Diabetic rats were treated with formulation (200 and 400 mg/kg) and metformin 250 mg/kg. Blood glucose levels were measured using blood glucose test strips with ACCU CHEK glucometer. Lipid profile and gluconeogenic enzymes were determined in normal and STZ-induced diabetic rats after oral administration of the PHF for 28 days. Histopathological changes in diabetic rat organs (pancreas, liver, and kidney) were also observed after PHF treatment.
  
  
  RESULTS
  Treatment of diabetic rats with PHF and metformin decreased plasma glucose and lipid profile levels. Blood glucose level showed significant reduction after 28 days of treatment with formulation at 200 and 400 mg/kg and in metformin. Formulation treated rats showed significant (P < 0.001) decrease in the activities of gluconeogenic enzymes. Histological examination of various organ tissues of normal control, diabetic control, and drug-treated rats revealed significant results. Treatment with PHF reverses the most blood and tissue changes toward the normal level.
  
  
  CONCLUSIONS
  These findings suggested the antihyperglycemic and antihyperlipidemic properties of the PHF and thus help in preventing future complications of diabetes.
authors.affiliation: Interdisciplinary Insitute of Indian System of Medicine, SRM University, Kattankulathur, Kancheepuram, Tamil Nadu, India.
authors.forename: N
authors.initials: N
authors.lastname: Subhasree
authors.affiliation: Interdisciplinary Insitute of Indian System of Medicine, SRM University, Kattankulathur, Kancheepuram, Tamil Nadu, India.
authors.forename: Ananthkumar
authors.initials: A
authors.lastname: Kamella
authors.affiliation: Interdisciplinary Insitute of Indian System of Medicine, SRM University, Kattankulathur, Kancheepuram, Tamil Nadu, India.
authors.forename: Ilango
authors.initials: I
authors.lastname: Kaliappan
authors.affiliation: Department of Kriya Sharir, Faculty of Ayurveda, Institute of Medical Sciences, Varanasi, Uttar Pradesh, India.
authors.forename: Aruna
authors.initials: A
authors.lastname: Agrawal
authors.affiliation: Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India.
authors.forename: Govind Prasad
authors.initials: GP
authors.lastname: Dubey
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001786:Blood Glucose / Q000187:drug effects; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy* / Q000503:physiopathology; D059305:Diet, High-Fat; D004305:Dose-Response Relationship, Drug; D005260:Female; D029760:Garcinia / Q000737:chemistry; D007004:Hypoglycemic Agents / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D000960:Hypolipidemic Agents / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D031563:Lagerstroemia / Q000737:chemistry; D008297:Male; D008687:Metformin / Q000494:pharmacology; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D049918:Salacia / Q000737:chemistry; D013311:Streptozocin
chemical_list: D001786:Blood Glucose; D007004:Hypoglycemic Agents; D000960:Hypolipidemic Agents; D010936:Plant Extracts; D013311:Streptozocin; D008687:Metformin
keywords: Metformin; polyherbal formulation; type 2 diabetes