title: CmWRKY15 Facilitates Alternaria tenuissima Infection of Chrysanthemum.
abstract: Abscisic acid (ABA) has an important role in the responses of plants to pathogens due to its ability to induce stomatal closure and interact with salicylic acid (SA) and jasmonic acid (JA). WRKY transcription factors serve as antagonistic or synergistic regulators in the response of plants to a variety of pathogens. Here, we demonstrated that CmWRKY15, a group IIa WRKY family member, was not transcriptionally activated in yeast cells. Subcellular localization experiments in which onion epidermal cells were transiently transfected with CmWRKY15 indicated that CmWRKY15 localized to the nucleus in vivo. The expression of CmWRKY15 could be markedly induced by the presence of Alternaria tenuissima inoculum in chrysanthemum. Furthermore, the disease severity index (DSI) data of CmWRKY15-overexpressing plants indicated that CmWRKY15 overexpression enhanced the susceptibility of chrysanthemum to A. tenuissima infection compared to controls. To illustrate the mechanisms by which CmWRKY15 regulates the response to A. tenuissima inoculation, the expression levels of ABA-responsive and ABA signaling genes, such as ABF4, ABI4, ABI5, MYB2, RAB18, DREB1A, DREB2A, PYL2, PP2C, RCAR1, SnRK2.2, SnRK2.3, NCED3A, NCED3B, GTG1, AKT1, AKT2, KAT1, KAT2, and KC1were compared between transgenic plants and controls. In summary, our data suggest that CmWRKY15 might facilitate A. tenuissima infection by antagonistically regulating the expression of ABA-responsive genes and genes involved in ABA signaling, either directly or indirectly.
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing, China.|Jiangsu Province Engineering Lab for Modern Facility Agriculture Technology & Equipment, Nanjing, China.
authors.forename: Qingqing
authors.initials: Q
authors.lastname: Fan
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing, China.
authors.forename: Aiping
authors.initials: A
authors.lastname: Song
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Xin
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing, China.
authors.forename: Sumei
authors.initials: S
authors.lastname: Chen
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing, China.
authors.forename: Jiafu
authors.initials: J
authors.lastname: Jiang
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing, China.
authors.forename: Yinjie
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing, China.
authors.forename: Xiran
authors.initials: X
authors.lastname: Li
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing, China.|Jiangsu Province Engineering Lab for Modern Facility Agriculture Technology & Equipment, Nanjing, China.
authors.forename: Fadi
authors.initials: F
authors.lastname: Chen
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology; D000528:Alternaria; D031202:Chrysanthemum / Q000145:classification / Q000187:drug effects / Q000235:genetics* / Q000382:microbiology*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D020022:Genetic Predisposition to Disease; D042541:Intracellular Space; D010641:Phenotype; D010802:Phylogeny; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology*; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified; D021381:Protein Transport; D013312:Stress, Physiological / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D015533:Transcriptional Activation
chemical_list: D010940:Plant Proteins; D014157:Transcription Factors; D000040:Abscisic Acid