title: Effects of genetic modifications and fermentation conditions on 2,3-butanediol production by alkaliphilic Bacillus subtilis.
abstract: Two recombinants of alkaliphilic Bacillus subtilis LOCK 1086, constructed via different strategies such as cloning the gene encoding bacterial hemoglobin from Vitreoscilla stercoraria (vhb) and overexpression of the gene encoding acetoin reductase/2,3-butanediol dehydrogenase (bdhA) from B. subtilis LOCK 1086, did not produce more 2,3-butanediol (2,3-BD) than the parental strain. In batch fermentations, this strain synthesized 9.46 g/L in 24 h and 12.80 g/L 2,3-BD in 46 h from sugar beet molasses and an apple pomace hydrolysate, respectively. 2,3-BD production by B. subtilis LOCK 1086 was significantly enhanced in fed-batch fermentations. The highest 2,3-BD concentration (75.73 g/L in 114 h, productivity of 0.66 g/L × h) was obtained in the sugar beet molasses-based medium with four feedings with glucose. In a medium based on the apple pomace hydrolysate with three feedings with sucrose, B. subtilis LOCK 1086 produced up to 51.53 g/L 2,3-BD (in 120 h, productivity of 0.43 g/L × h).
authors.affiliation: Institute of Technical Biochemistry, Lodz University of Technology, Stefanowskiego Street 4/10, 90-924, Lodz, Poland. aneta.bialkowska@p.lodz.pl.
authors.forename: Aneta M
authors.initials: AM
authors.lastname: Białkowska
authors.affiliation: Institute of Technical Biochemistry, Lodz University of Technology, Stefanowskiego Street 4/10, 90-924, Lodz, Poland.
authors.forename: Marzena
authors.initials: M
authors.lastname: Jędrzejczak-Krzepkowska
authors.affiliation: Institute of Technical Biochemistry, Lodz University of Technology, Stefanowskiego Street 4/10, 90-924, Lodz, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Gromek
authors.affiliation: Institute of Technical Biochemistry, Lodz University of Technology, Stefanowskiego Street 4/10, 90-924, Lodz, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Krysiak
authors.affiliation: Institute of Technical Biochemistry, Lodz University of Technology, Stefanowskiego Street 4/10, 90-924, Lodz, Poland.
authors.forename: Barbara
authors.initials: B
authors.lastname: Sikora
authors.affiliation: Institute of Technical Biochemistry, Lodz University of Technology, Stefanowskiego Street 4/10, 90-924, Lodz, Poland.
authors.forename: Halina
authors.initials: H
authors.lastname: Kalinowska
authors.affiliation: Institute of Technical Biochemistry, Lodz University of Technology, Stefanowskiego Street 4/10, 90-924, Lodz, Poland.
authors.forename: Celina
authors.initials: C
authors.lastname: Kubik
authors.affiliation: Thünen Institute of Wood Research, Leuschnerstrasse 91, 21031, Hamburg, Germany.
authors.forename: Fokko
authors.initials: F
authors.lastname: Schütt
authors.affiliation: Institute of Technical Biochemistry, Lodz University of Technology, Stefanowskiego Street 4/10, 90-924, Lodz, Poland.
authors.forename: Marianna
authors.initials: M
authors.lastname: Turkiewicz
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics* / Q000378:metabolism*; D027461:Beta vulgaris / Q000378:metabolism; D002072:Butylene Glycols / Q000378:metabolism*; D005285:Fermentation; D015870:Gene Expression; D007220:Industrial Waste; D027845:Malus / Q000378:metabolism; D060847:Metabolic Engineering / Q000379:methods*; D053858:Metabolic Networks and Pathways / Q000235:genetics*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D011995:Recombination, Genetic; D020589:Vitreoscilla / Q000201:enzymology / Q000235:genetics
chemical_list: D002072:Butylene Glycols; D007220:Industrial Waste; D011994:Recombinant Proteins; C026978:2,3-butylene glycol
keywords: 2,3-Butanediol; Acetoin reductase/2,3-butanediol dehydrogenase (bdhA); B. subtilis; Fermentation; Vitreoscilla stercoraria hemoglobin (vhb)