title: The progressive changes of filamentous actin cytoskeleton in the hippocampal neurons after pilocarpine-induced status epilepticus.
abstract: In a previous study, we reported a persistent reduction of F-actin puncta but a compensating increase in puncta size in the mouse hippocampus at 2 months after pilocarpine-induced status epilepticus (Epilepsy Res. 108 (2014), 379-389). However, the F-actin changes during the period of epileptogenesis remain unknown. This study was designed to examine the temporal and spatial changes of F-actin during the period of epileptogenesis in a pilocarpine-induced epilepsy model, primarily by the histological and TUNEL evaluation of cell loss, phalloidin detection of F-actin, and immunohistochemical analysis of glial reactions. The results demonstrated that F-actin continued to decrease after pilocarpine treatment, which was consistent in its time course with hippocampal neuronal death. Within different hippocampal subfields, the spatial changes of F-actin exhibited similar features. First, the F-actin puncta decreased in number. Thereafter, F-actin was transiently aggregated in dendritic shafts and neuronal cell bodies and eventually was completely lost in the degenerated neurons. The progressive changes of F-actin in the degenerating neurons reported in this study may help to elucidate a cytoskeletal mechanism that may link to the delayed cell loss that occurs during epileptogenesis.
authors.affiliation: Department of Histology and Embryology, College of Basic Medical Sciences, Norman Bethune Health Science Center of Jilin University, Jilin Province 130021, PR China.
authors.forename: Tianqing
authors.initials: T
authors.lastname: Xiong
authors.affiliation: Department of Histology and Embryology, College of Basic Medical Sciences, Norman Bethune Health Science Center of Jilin University, Jilin Province 130021, PR China.
authors.forename: Jiamei
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Histology and Embryology, College of Basic Medical Sciences, Norman Bethune Health Science Center of Jilin University, Jilin Province 130021, PR China.
authors.forename: Gaoyue
authors.initials: G
authors.lastname: Dai
authors.affiliation: Department of Anatomy, College of Basic Medical Sciences, Norman Bethune Health Science Center of Jilin University, Jilin Province 130021, PR China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Hou
authors.affiliation: Laboratory Teaching Center of Basic Medicine, Norman Bethune Health Science Center of Jilin University, Jilin Province 130021, PR China.
authors.forename: Baihong
authors.initials: B
authors.lastname: Tan
authors.affiliation: Pediatric Neurology, First Hospital of Jilin University, Changchun, Jilin Province 130021, PR China.
authors.forename: Yanfeng
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Histology and Embryology, College of Basic Medical Sciences, Norman Bethune Health Science Center of Jilin University, Jilin Province 130021, PR China. Electronic address: lsl@jlu.edu.cn.
authors.forename: Shulei
authors.initials: S
authors.lastname: Li
authors.affiliation: Nursing College, Beihua University, 3999 Huashan Road, Jilin 132013, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Song
authors.affiliation: Department of Anatomy, College of Basic Medical Sciences, Norman Bethune Health Science Center of Jilin University, Jilin Province 130021, PR China. Electronic address: haiyan@jlu.edu.cn.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Neurology, Fourth Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province 150001, PR China.
authors.forename: Yongnan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Histology and Embryology, College of Basic Medical Sciences, Norman Bethune Health Science Center of Jilin University, Jilin Province 130021, PR China. Electronic address: liyanchao@jlu.edu.cn.
authors.forename: Yanchao
authors.initials: Y
authors.lastname: Li
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism*; D000199:Actins / Q000378:metabolism*; D000704:Analysis of Variance; D000818:Animals; D002135:Calcium-Binding Proteins / Q000378:metabolism; D002478:Cells, Cultured; D004195:Disease Models, Animal; D005786:Gene Expression Regulation / Q000187:drug effects; D006624:Hippocampus / Q000187:drug effects / Q000473:pathology*; D020287:In Situ Nick-End Labeling; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008840:Microfilament Proteins / Q000378:metabolism; D018721:Muscarinic Agonists / Q000633:toxicity; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009474:Neurons / Q000187:drug effects / Q000473:pathology*; D010862:Pilocarpine / Q000633:toxicity; D013226:Status Epilepticus / Q000139:chemically induced / Q000473:pathology*; D013997:Time Factors
chemical_list: D000199:Actins; C491049:Aif1 protein, mouse; D002135:Calcium-Binding Proteins; D008840:Microfilament Proteins; D018721:Muscarinic Agonists; D009419:Nerve Tissue Proteins; D010862:Pilocarpine
keywords: Filamentous actin; Hippocampus; Pilocarpine; Status epilepticus