title: Studies of Highly-Ordered Heterodiantennary Mannose/Glucose-Functionalized Polymers and Concanavalin A Protein Interactions Using Isothermal Titration Calorimetry.
abstract: Preparations of the highly ordered monoantennary, homofunctional diantennary, and heterofunctional diantennary neoglycopolymers of α-d-mannose and β-d-glucose residues were achieved via ring-opening metathesis polymerization. Isothermal titration calorimetry measurements of these synthetic neoglycopolymers with Concanavalin A (Con A), revealed that heterofunctional diantennary architectures bearing both α-mannose and nonbinding β-glucose units, poly(Man-Glc), binds to Con A (Ka = 16.1 × 10(6) M(-1)) comparably to homofunctional diantennary neoglycopolymer (Ka = 30 × 10(6) M(-1)) bearing only α-mannose unit, poly(Man-Man). In addition, poly(Man-Glc) neoglycopolymer shows a nearly 5-fold increasing in binding affinity compared to monoantennary neoglycopolymer, poly(Man). Although the exact mechanism for the high binding affinity of poly(Man-Glc) to Con A is unclear, we hypothesize that the α-mannose bound to Con A might facilitate interaction of β-glucose with the extended binding site of Con A due to the close proximity of β-glucose to α-mannose residues in the designed polymerizable scaffold.
authors.affiliation: Department of Chemistry, University of Iowa, Iowa 52242, United States.
authors.forename: Ravi S
authors.initials: RS
authors.lastname: Loka
authors.affiliation: Department of Chemistry, University of Iowa, Iowa 52242, United States.
authors.forename: Matthew S
authors.initials: MS
authors.lastname: McConnell
authors.affiliation: Department of Chemistry, University of Iowa, Iowa 52242, United States.
authors.forename: Hien M
authors.initials: HM
authors.lastname: Nguyen
mesh_terms: D001665:Binding Sites; D002151:Calorimetry / Q000379:methods; D002236:Carbohydrate Conformation; D003208:Concanavalin A / Q000737:chemistry*; D005947:Glucose / Q000737:chemistry*; D007700:Kinetics; D008358:Mannose / Q000737:chemistry*; D058105:Polymerization
chemical_list: D003208:Concanavalin A; D005947:Glucose; D008358:Mannose