title: Analysis of the reaction products from micro-vial pyrolysis of the mixture glucose/proline and of a tobacco leaf extract:Search for Amadori intermediates.
abstract: Micro-vial pyrolysis (PyroVial) was used to study the production of compounds important for the aroma of heat-treated natural products such as tobacco. Firstly, a mixture of glucose and proline was pyrolyzed as model, as this sugar and amino acid are also abundant in tobacco leaf (Nicotiana tobacum L.). The pyrolysate was analyzed using headspace-GC–MS, liquid injection GC–MS and LC–MS. Next, micro-vial pyrolysis in combination with LC–MS was applied to tobacco leaf extract. Using MS deconvolution, molecular feature extraction and differential analysis it was possible to identify Amadori intermediates of the Maillard reaction in the tobacco leaf extract. The intermediate disappeared as was the case for 1-deoxy-1-prolino-β-d-fructose or the concentration decreased in the pyrolysate compared to the original extract such as for the 1-deoxy-1-[2-(3-pyridyl)-1-pyrrolidinyl]-β-d-fructose isomers indicating that Amadori intermediates are important precursors for aroma compound formation.
authors.affiliation: Japan Tobacco Inc., Tobacco Science Research Center, 6-2 Umegaoka, Aoba-ku, 227-8512 Yokohama, Japan; The United Graduate School of Agricultural Sciences, Ehime University, 3-5-7 Tarumi, 790-8566 Matsuyama, Japan. Electronic address: kazuhisa.mitsui@jt.com.
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Mitsui
authors.affiliation: Research Institute for Chromatography, 26 President Kennedypark, 8500 Kortrijk, Belgium.
authors.forename: Frank
authors.initials: F
authors.lastname: David
authors.affiliation: Research Institute for Chromatography, 26 President Kennedypark, 8500 Kortrijk, Belgium.
authors.forename: Bart
authors.initials: B
authors.lastname: Tienpont
authors.affiliation: Research Institute for Chromatography, 26 President Kennedypark, 8500 Kortrijk, Belgium.
authors.forename: Koen
authors.initials: K
authors.lastname: Sandra
authors.affiliation: Gerstel K.K., 1-3-1 Nakane, Meguro-ku, 152-0031 Tokyo, Japan.
authors.forename: Nobuo
authors.initials: N
authors.lastname: Ochiai
authors.affiliation: The United Graduate School of Agricultural Sciences, Ehime University, 3-5-7 Tarumi, 790-8566 Matsuyama, Japan; Department of Applied Biological Science, Faculty of Agriculture, Kagawa University, 2393 Miki-cho, 761-0795 Kagawa, Japan.
authors.forename: Hirotoshi
authors.initials: H
authors.lastname: Tamura
authors.affiliation: Research Institute for Chromatography, 26 President Kennedypark, 8500 Kortrijk, Belgium.
authors.forename: Pat
authors.initials: P
authors.lastname: Sandra
mesh_terms: D005632:Fructose / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry; D005947:Glucose / Q000378:metabolism*; D015416:Maillard Reaction; D010936:Plant Extracts / Q000737:chemistry*; D011392:Proline / Q000378:metabolism*; D014026:Nicotiana / Q000737:chemistry*
chemical_list: D010936:Plant Extracts; D005632:Fructose; D011392:Proline; D005947:Glucose