title: Aptamer induced assembly of fluorescent nitrogen-doped carbon dots on gold nanoparticles for sensitive detection of AFB1.
abstract: Novel fluorescent nitrogen-doped carbon dots (N,C-dots) were synthesized and assembled on aptamer modified gold nanoparticles (Aptamer/AuNPs) for the super sensitive detection of aflatoxin B1 (AFB1). Positively charged N,C-dots were synthesized by the hydrothermal treatment of pancreatin. The prepared N,C-dots were assembled on aptamer/AuNPs by electrostatic interactions. The fluorescence of the N,C-dots was efficiently quenched. When AFB1 was added to the assay solution, specific interactions between AFB1 and the aptamer caused release of the N,C-dots. The fluorescence of the N,C-dots recovered and the intensity increase could be used to calculate the amount of AFB1 added. The assay exhibits super-high sensitivity with a detection limit of 5 pg/mL (16 pM) and a wide range of linear response of 5 pg/mL to 2.00 ng/mL. A novel aptasensor is thus successfully constructed, it provides an efficient way for sensitive AFB1 sensing as well as a new technique for aptamer based novel sensor construction.
authors.affiliation: Institute for Clean Energy & Advanced Materials, Faculty of Materials and Energy, Southwest University, Chongqing 400715, China; Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Wuhan University, Wuhan 430072, China; Chongqing Key Laboratory for Advanced Materials and Technologies of Clean Energies, Chongqing 400715, China; Chongqing Engineering Research Center for Rapid diagnosis of Fatal Diseases, Chongqing 400715, China. Electronic address: bwang@swu.edu.cn.
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.affiliation: Institute for Clean Energy & Advanced Materials, Faculty of Materials and Energy, Southwest University, Chongqing 400715, China; Chongqing Key Laboratory for Advanced Materials and Technologies of Clean Energies, Chongqing 400715, China; Chongqing Engineering Research Center for Rapid diagnosis of Fatal Diseases, Chongqing 400715, China.
authors.forename: Yanfen
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Institute for Clean Energy & Advanced Materials, Faculty of Materials and Energy, Southwest University, Chongqing 400715, China; Chongqing Key Laboratory for Advanced Materials and Technologies of Clean Energies, Chongqing 400715, China; Chongqing Engineering Research Center for Rapid diagnosis of Fatal Diseases, Chongqing 400715, China.
authors.forename: Yuanya
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Institute for Clean Energy & Advanced Materials, Faculty of Materials and Energy, Southwest University, Chongqing 400715, China; Chongqing Key Laboratory for Advanced Materials and Technologies of Clean Energies, Chongqing 400715, China; Chongqing Engineering Research Center for Rapid diagnosis of Fatal Diseases, Chongqing 400715, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Weng
authors.affiliation: Institute for Clean Energy & Advanced Materials, Faculty of Materials and Energy, Southwest University, Chongqing 400715, China; Chongqing Key Laboratory for Advanced Materials and Technologies of Clean Energies, Chongqing 400715, China; Chongqing Engineering Research Center for Rapid diagnosis of Fatal Diseases, Chongqing 400715, China.
authors.forename: Yingshuai
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Institute for Clean Energy & Advanced Materials, Faculty of Materials and Energy, Southwest University, Chongqing 400715, China; Chongqing Key Laboratory for Advanced Materials and Technologies of Clean Energies, Chongqing 400715, China; Chongqing Engineering Research Center for Rapid diagnosis of Fatal Diseases, Chongqing 400715, China.
authors.forename: Zhisong
authors.initials: Z
authors.lastname: Lu
authors.affiliation: Institute for Clean Energy & Advanced Materials, Faculty of Materials and Energy, Southwest University, Chongqing 400715, China; Chongqing Key Laboratory for Advanced Materials and Technologies of Clean Energies, Chongqing 400715, China; Chongqing Engineering Research Center for Rapid diagnosis of Fatal Diseases, Chongqing 400715, China.
authors.forename: Chang Ming
authors.initials: CM
authors.lastname: Li
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry Chinese Academy of Sciences, Changchun 130022, China. Electronic address: congyu@ciac.ac.cn.
authors.forename: Cong
authors.initials: C
authors.lastname: Yu
mesh_terms: D016604:Aflatoxin B1 / Q000737:chemistry / Q000302:isolation & purification*; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D010367:Arachis / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D002244:Carbon / Q000737:chemistry; D055664:Electrochemical Techniques / Q000379:methods*; D005453:Fluorescence; D006046:Gold / Q000737:chemistry; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry; D009584:Nitrogen / Q000737:chemistry; D003313:Zea mays / Q000737:chemistry
chemical_list: D052157:Aptamers, Nucleotide; D002244:Carbon; D006046:Gold; D016604:Aflatoxin B1; D009584:Nitrogen
keywords: AFB(1); Aptamer; AuNPs; Carbon dots; Fluorescence