title: [Effect of cyclosporine A on TGF-β/Smad3 signaling in rat gingival fibroblasts].
abstract: OBJECTIVE
  The aim of the study was to investigate the effect of cyclosporine A (CsA) on TGF-β/Smad3 signaling in rat gingival fibroblasts and to explore the mechanism of CsA induced gingival overgrowth.
  
  
  METHODS
  Healthy Sprague-Dawley rat gingival fibroblasts were cultured with different concentrations of CsA and the cell proliferations were assessed with CCK-8 assay. The mRNA levels of TGF-β1, Smad3 and collagen I were measured by real-time PCR. The protein level of TGF-β1, Smad3, p-Smad3 and collagen I were determined using western blot and immumofluorescence. Cell migration ability was detected by cell wound scratch assay. The data was analyzed with SPSS 20.0 software package.
  
  
  RESULTS
  The use of 200 ng/mL CsA stimulated proliferation and migration of gingival fibroblasts. The mRNA levels of TGF-β1 and collagen I were significantly promoted after CsA exposure. The protein syntheses of TGF-β1, p-Smad3 and Collagen I were also increased by CsA stimulation.
  
  
  CONCLUSIONS
  CsA may activate TGF-β/Smad3 signaling pathway, thus promoting the proliferation and migration of rat gingival fibroblasts as well as collagen accumulation, which eventually lead to gingival overgrowth.
authors.affiliation: Department of Stomatology, Zhongshan Hospital, Fudan University. Shanghai 200032, China.E-mail:12211210043@fudan.edu.cn.
authors.forename: Jia-lu
authors.initials: JL
authors.lastname: Chen
authors.forename: You-cheng
authors.initials: YC
authors.lastname: Yu
authors.forename: Yi-ming
authors.initials: YM
authors.lastname: Gong
authors.forename: Fei
authors.initials: F
authors.lastname: Yang
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D003094:Collagen; D016572:Cyclosporine / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005347:Fibroblasts / Q000378:metabolism*; D005881:Gingiva / Q000378:metabolism*; D019214:Gingival Overgrowth; D012333:RNA, Messenger; D051381:Rats; D017207:Rats, Sprague-Dawley; D051900:Smad3 Protein / Q000378:metabolism*; D016212:Transforming Growth Factor beta / Q000378:metabolism*
chemical_list: D004791:Enzyme Inhibitors; D012333:RNA, Messenger; D051900:Smad3 Protein; C497787:Smad3 protein, rat; D016212:Transforming Growth Factor beta; D016572:Cyclosporine; D003094:Collagen