title: Lipoprotein Profiles in Class III Obese Caucasian and African American Women with Nonalcoholic Fatty Liver Disease.
abstract: Triglyceride content in the liver is regulated by the uptake, production and elimination of lipoproteins, and derangements in these processes contribute to nonalcoholic fatty liver disease (NAFLD). Previous studies show a direct relationship between intrahepatic fat and production of apolipoprotein B100 (apoB100) containing particles, VLDL and LDL, but little consensus exists regarding changes in lipoprotein production in the development of simple steatosis (SS) versus nonalcoholic steatohepatitis (NASH). Further, ethnic variations in lipoproteins among SS and NASH are unknown as is how such variations might contribute to the differential prevalence of disease among Caucasians versus African Americans. In this study, we assessed plasma lipoprotein profiles by nuclear magnetic resonance (NMR) spectroscopy in 70 non-diabetic class III obese females recruited from the surgical weight loss clinic. Of these, 51 females were stratified by biopsy-staged NAFLD severity (histologically normal, SS, or NASH). NASH females displayed increased circulating triglycerides and increased VLDL particle number and size relative to those with histologically normal livers, while total and large LDL concentration decreased in SS versus NASH and correlated with increased insulin resistance (via HOMA2-IR). When Caucasian women were examined alone (n = 41), VLDL and triglycerides increased between normal and SS, while total LDL and apoB100 decreased between SS and NASH along with increased insulin resistance. Compared to Caucasians with SS, African American women with SS displayed reduced triglycerides, VLDL, and small LDL and a more favorable small to large HDL ratio despite having increased BMI and HOMA2-IR. These findings suggest that ApoB100 and lipoprotein subclass particle number and size can delineate steatosis from NASH in obese Caucasian females, but should be interpreted with caution in other ethnicities as African Americans with SS display relatively improved lipoprotein profiles. This may reflect variation in the relationship between dyslipidemia and NAFLD progression across gender and ethnicity.
authors.affiliation: Department of Surgery, Vanderbilt University Medical Center, Nashville, Tennessee, United States of America.
authors.forename: Anna E
authors.initials: AE
authors.lastname: Garcia
authors.affiliation: Le Bonheur Children's Foundation Research Center, Memphis, Tennessee 38103, United States of America.
authors.forename: Nader
authors.initials: N
authors.lastname: Kasim
authors.affiliation: Department of Surgery, Vanderbilt University Medical Center, Nashville, Tennessee, United States of America.
authors.forename: Robyn A
authors.initials: RA
authors.lastname: Tamboli
authors.affiliation: Department of Pathology, Vanderbilt University Medical Center, Nashville, Tennessee, United States of America.
authors.forename: Raul S
authors.initials: RS
authors.lastname: Gonzalez
authors.affiliation: Department of Surgery, Vanderbilt University Medical Center, Nashville, Tennessee, United States of America.
authors.forename: Joseph
authors.initials: J
authors.lastname: Antoun
authors.affiliation: Department of Surgery, Vanderbilt University Medical Center, Nashville, Tennessee, United States of America.
authors.forename: Emily A
authors.initials: EA
authors.lastname: Eckert
authors.affiliation: Department of Surgery, Vanderbilt University Medical Center, Nashville, Tennessee, United States of America.
authors.forename: Pamela A
authors.initials: PA
authors.lastname: Marks-Shulman
authors.affiliation: Department of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee, United States of America.
authors.forename: Julia
authors.initials: J
authors.lastname: Dunn
authors.affiliation: Columbia University College of Physicians and Surgeons, New York, New York, United States of America.
authors.forename: Julia
authors.initials: J
authors.lastname: Wattacheril
authors.affiliation: Central Michigan University, College of Medicine, Mount Pleasant, Michigan, United States of America.
authors.forename: Taylor
authors.initials: T
authors.lastname: Wallen
authors.affiliation: Department of Surgery, Vanderbilt University Medical Center, Nashville, Tennessee, United States of America.
authors.forename: Naji N
authors.initials: NN
authors.lastname: Abumrad
authors.affiliation: Department of Surgery, Vanderbilt University Medical Center, Nashville, Tennessee, United States of America.
authors.forename: Charles Robb
authors.initials: CR
authors.lastname: Flynn
mesh_terms: D000328:Adult; D001741:Black or African American; D000886:Anthropometry; D053299:Apolipoprotein B-100 / Q000378:metabolism; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D018450:Disease Progression; D005260:Female; D006801:Humans; D007333:Insulin Resistance; D008074:Lipoproteins / Q000097:blood*; D009682:Magnetic Resonance Spectroscopy; D008875:Middle Aged; D065626:Non-alcoholic Fatty Liver Disease / Q000097:blood / Q000150:complications* / Q000208:ethnology; D009765:Obesity / Q000097:blood / Q000150:complications* / Q000208:ethnology; D015995:Prevalence; D011446:Prospective Studies; D014280:Triglycerides / Q000097:blood; D044465:White People; D055815:Young Adult
chemical_list: C569471:APOB protein, human; D053299:Apolipoprotein B-100; D015415:Biomarkers; D008074:Lipoproteins; D014280:Triglycerides