title: Design and synthesis of fluorescence-labeled nucleotide with a cleavable azo linker for DNA sequencing.
abstract: A cleavable azo linker was synthesized and reacted with 5-(6)-carboxytetramethyl rhodamine succinimidyl ester, followed by further reactions with di(N-succinimidyl) carbonate and 5-(3-amino-1-propynyl)-2'-deoxyuridine 5'-triphosphate [dUTP(AP3)] to obtain the terminal product dUTP-azo linker-TAMRA as a potential reversible terminator for DNA sequencing by synthesis with no need for 3'-OH blocking.
authors.affiliation: Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine, Shanghai Jiao Tong University, Shanghai 200240, China. yumeishen@sjtu.edu.cn.
authors.forename: Lianjiang
authors.initials: L
authors.lastname: Tan
authors.affiliation: Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine, Shanghai Jiao Tong University, Shanghai 200240, China. yumeishen@sjtu.edu.cn and Key Laboratory for Advanced Materials and Institute of Fine Chemicals, School of Chemistry & Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China.
authors.forename: Yazhi
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine, Shanghai Jiao Tong University, Shanghai 200240, China. yumeishen@sjtu.edu.cn.
authors.forename: Qinglai
authors.initials: Q
authors.lastname: Yang
authors.affiliation: Bio-ID Center, School of Bio-medical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Li
authors.affiliation: Key Laboratory for Advanced Materials and Institute of Fine Chemicals, School of Chemistry & Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China.
authors.forename: Xin-Yan
authors.initials: XY
authors.lastname: Wu
authors.affiliation: College of Chemistry, Beijing Normal University, Beijing 100875, China and Department of Chemistry, University at Buffalo, State University of New York, Buffalo, NY 14260, USA.
authors.forename: Bing
authors.initials: B
authors.lastname: Gong
authors.affiliation: Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine, Shanghai Jiao Tong University, Shanghai 200240, China. yumeishen@sjtu.edu.cn.
authors.forename: Yu-Mei
authors.initials: YM
authors.lastname: Shen
authors.affiliation: Bio-ID Center, School of Bio-medical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Zhifeng
authors.initials: Z
authors.lastname: Shao
mesh_terms: D001391:Azo Compounds / Q000737:chemistry*; D015195:Drug Design; D005453:Fluorescence; D015394:Molecular Structure; D009711:Nucleotides / Q000138:chemical synthesis* / Q000737:chemistry*; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D001391:Azo Compounds; D009711:Nucleotides