title: Phytoestrogens Activate the Estrogen Receptor in HepG2 Cells.
abstract: Phytoestrogens are popular alternatives to estrogen therapy however their effects on hemostasis in postmenopausal women are unknown. This chapter describes a protocol to determine the effect of the phytoestrogens genistein, daidzein and equol, on the expression of key genes from the hemostatic system in human hepatocyte cell models and to determine the role of estrogen receptors in mediating any response seen using in vitro culture systems and Taqman(®) gene expression analysis.
authors.affiliation: Department of Paediatrics, The Coombe Women and Infants University Hospital & Trinity College, Dublin 8, Ireland. kellyl37@tcd.ie.
authors.forename: Lynne A
authors.initials: LA
authors.lastname: Kelly
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism*; D060754:Equol / Q000494:pharmacology*; D004958:Estradiol / Q000494:pharmacology; D047628:Estrogen Receptor alpha / Q000819:agonists* / Q000235:genetics / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D019833:Genistein / Q000494:pharmacology*; D056945:Hep G2 Cells; D006801:Humans; D007529:Isoflavones / Q000494:pharmacology*; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism*; D048789:Phytoestrogens / Q000494:pharmacology*; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019914:Taq Polymerase / Q000378:metabolism; D014162:Transfection; D057188:Workflow
chemical_list: D047628:Estrogen Receptor alpha; D007529:Isoflavones; D048789:Phytoestrogens; D004958:Estradiol; D060754:Equol; C004742:daidzein; D019833:Genistein; D019914:Taq Polymerase
keywords: 17β-Estradiol; Daidzein; Equol; Estrogen receptor; Genistein; Hep89; HepG2; Phytoestrogens