title: A Simple and Safe Protocol for Preparing Brucella Samples for Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry Analysis.
abstract: We describe a simple protocol to inactivate the biosafety level 3 (BSL3) pathogens Brucella prior to their analysis by matrix-assisted laser desorption ionization-time of flight mass spectrometry. This method is also effective for several other bacterial pathogens and allows storage, and eventually shipping, of inactivated samples; therefore, it might be routinely applied to unidentified bacteria, for the safety of laboratory workers.
authors.affiliation: INSERM, U1047, UFR de Médecine, Nîmes, France Université de Montpellier, U1047, Nîmes, France.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Mesureur
authors.affiliation: INSERM, U1047, UFR de Médecine, Nîmes, France Université de Montpellier, U1047, Nîmes, France.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Ranaldi
authors.affiliation: bioMérieux, R&D Microbiology, La Balme-les-Grottes, France.
authors.forename: Valérie
authors.initials: V
authors.lastname: Monnin
authors.affiliation: bioMérieux, R&D Microbiology, La Balme-les-Grottes, France.
authors.forename: Victoria
authors.initials: V
authors.lastname: Girard
authors.affiliation: bioMérieux, R&D Microbiology, La Balme-les-Grottes, France.
authors.forename: Sandrine
authors.initials: S
authors.lastname: Arend
authors.affiliation: bioMérieux, R&D Microbiology, La Balme-les-Grottes, France.
authors.forename: Martin
authors.initials: M
authors.lastname: Welker
authors.affiliation: INSERM, U1047, UFR de Médecine, Nîmes, France Université de Montpellier, U1047, Nîmes, France.
authors.forename: David
authors.initials: D
authors.lastname: O'Callaghan
authors.affiliation: INSERM, U1047, UFR de Médecine, Nîmes, France Université de Montpellier, U1047, Nîmes, France Service de Microbiologie, CHU Caremeau, Nîmes, France.
authors.forename: Jean-Philippe
authors.identifier: http://orcid.org/0000-0002-9484-0304
authors.initials: JP
authors.lastname: Lavigne
authors.affiliation: INSERM, U1047, UFR de Médecine, Nîmes, France Université de Montpellier, U1047, Nîmes, France anne.keriel@inserm.fr.
authors.forename: Anne
authors.initials: A
authors.lastname: Keriel
mesh_terms: D002002:Brucella / Q000187:drug effects; D002006:Brucellosis / Q000175:diagnosis* / Q000382:microbiology*; D006801:Humans; D050296:Microbial Viability / Q000187:drug effects; D012997:Solvents; D013048:Specimen Handling / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
chemical_list: D012997:Solvents