title: Differentiation and Cell-Cell Interactions of Neural Progenitor Cells Transplanted into Intact Adult Brain.
abstract: We studied the behavior and cell-cell interactions of embryonic brain cell from GFP-reporter mice after their transplantation into the intact adult brain. Fragments or cell suspensions of fetal neocortical cells at different stages of development were transplanted into the neocortex and striatum of adult recipients. Even in intact brain, the processes of transplanted neurons formed extensive networks in the striatum and neocortical layers I and V-VI. Processes of transplanted cells at different stages of development attained the rostral areas of the frontal cortex and some of them reached the internal capsule. However, the cells transplanted in suspension had lower process growth potency than cells from tissue fragments. Tyrosine hydroxylase fibers penetrated from the recipient brain into grafts at both early and late stages of development. Our experiments demonstrated the formation of extensive reciprocal networks between the transplanted fetal neural cells and recipient brain neurons even in intact brain.
authors.affiliation: N. K. Kol'tsov Institute of Developmental Biology, Russian Academy of Sciences, Moscow, Russia. transpl@hotmail.com.
authors.forename: K K
authors.initials: KK
authors.lastname: Sukhinich
authors.affiliation: N. K. Kol'tsov Institute of Developmental Biology, Russian Academy of Sciences, Moscow, Russia.|N. I. Pirogov National Russian Research Medical University, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: A V
authors.initials: AV
authors.lastname: Kosykh
authors.affiliation: N. K. Kol'tsov Institute of Developmental Biology, Russian Academy of Sciences, Moscow, Russia.|V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: M A
authors.initials: MA
authors.lastname: Aleksandrova
mesh_terms: D064591:Allografts; D000818:Animals; D001253:Astrocytes / Q000166:cytology; D016380:Brain Tissue Transplantation; D002450:Cell Communication; D003342:Corpus Striatum / Q000601:surgery; D005260:Female; D016332:Fetal Tissue Transplantation; D017930:Genes, Reporter; D005911:Gliosis / Q000209:etiology / Q000473:pathology; D049452:Green Fluorescent Proteins / Q000032:analysis; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D019579:Neocortex / Q000601:surgery; D009419:Nerve Tissue Proteins / Q000032:analysis; D058953:Neural Stem Cells / Q000637:transplantation*; D055495:Neurogenesis; D009474:Neurons / Q000166:cytology / Q000201:enzymology; D011183:Postoperative Complications / Q000209:etiology / Q000473:pathology; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis
chemical_list: D009419:Nerve Tissue Proteins; D049452:Green Fluorescent Proteins; D014446:Tyrosine 3-Monooxygenase
keywords: GFP mouse; cell processes; immunohistochemistry; neurotransplantation; undifferentiated cells