title: Quantitation of Aldosterone in Serum or Plasma Using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS).
abstract: Accurate determination of serum and plasma aldosterone is essential for screening, diagnosis, and subtype classification of primary aldosteronism (PA). Its measurement is also used in the investigation of adrenal incidentaloma, adrenal carcinoma, Addison's disease, congenital adrenal hyperplasia, renal artery stenosis, and renal tubular channelopathies. We describe a simple and robust method for the accurate and precise measurement of aldosterone in serum or plasma using liquid chromatography and tandem mass spectrometry (LC-MS/MS). After addition of internal standard, aldosterone is extracted from serum samples using supported liquid extraction (SLE) with methyl-t-butyl ether (MtBE). The MtBE is evaporated to dryness and sample is reconstituted with mobile phase before injection onto the LC-MS/MS and quantitation using an 8-point calibration curve. The assay calibration range is approximately 50-6500 pmol/L (0.16-234 ng/dL) with total imprecision between 6.8 and 4.1 % for concentrations between about 50 and 1000 pmol/L respectively.
authors.affiliation: Department of Pathology and Laboratory Medicine, University of British Columbia, Rm. G227 - 2211 Wesbrook Mall, Vancouver, BC, V6T 2B5, Canada.|Department of Pathology and Laboratory Medicine, St. Paul's Hospital, 1081 Burrard St., Vancouver, BC, V6Z 1Y6, Canada.
authors.forename: J Grace
authors.initials: JG
authors.lastname: Van Der Gugten
authors.affiliation: Department of Pathology and Laboratory Medicine, University of British Columbia, Rm. G227 - 2211 Wesbrook Mall, Vancouver, BC, V6T 2B5, Canada. dtholmes@mail.ubc.ca.|Department of Pathology and Laboratory Medicine, St. Paul's Hospital, 1081 Burrard St., Vancouver, BC, V6Z 1Y6, Canada. dtholmes@mail.ubc.ca.
authors.forename: Daniel T
authors.initials: DT
authors.lastname: Holmes
mesh_terms: D000450:Aldosterone / Q000097:blood*; D053000:Analytic Sample Preparation Methods; D001774:Blood Chemical Analysis / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000450:Aldosterone
keywords: Aldosterone; Hypokalemia; Mass spectrometry; Mineralocorticoid hypertension; Plasma renin activity; Primary aldosteronism; Secondary hypertension