title: [FUNCTIONAL STATE OF SCORPION FISH SCORPAENA PORCUS L. NUCLEATED ERYTHROCYTES UNDER DOSED HYPOXIA].
abstract: Hypoxia is a wide spread phenomena throughout the world's aquatic systems. This paper investigates the functional state of scorpion fish red blood cells under acute hypoxia between 0.57-8.17 mg O2/l (in vitro) by assessing of the fluorescence of Rhodamine 123 (R123) and Fluorescein diacetate (FDA). Acute hypoxia induced a significant increase in the fluorescence of both fluorochromes (R123 - 12-60, FDA - 30-184%). This reaction is based on a probable decrease in erythrocyte membrane permeability. The reduction of functional ionic channels in red blood cells delays FDA in the cytoplasm and lowers the energy costs required for transmembrane cationic gradient maintenance, which reflects an intensification of R123 fluorescence.
authors.forename: A Yu
authors.initials: AY
authors.lastname: Andreyeva
authors.forename: A A
authors.initials: AA
authors.lastname: Soldatov
authors.forename: V S
authors.initials: VS
authors.lastname: Mukhanov
mesh_terms: D000818:Animals; D015687:Cell Hypoxia; D002463:Cell Membrane Permeability; D004900:Erythroblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D004910:Erythrocyte Membrane / Q000187:drug effects* / Q000378:metabolism; D005434:Flow Cytometry; D005452:Fluoresceins / Q000737:chemistry / Q000378:metabolism; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism; D017136:Ion Transport; D010100:Oxygen / Q000494:pharmacology*; D010473:Perciformes; D061251:Primary Cell Culture; D020112:Rhodamine 123 / Q000737:chemistry / Q000378:metabolism
chemical_list: D005452:Fluoresceins; D005456:Fluorescent Dyes; D020112:Rhodamine 123; D010100:Oxygen; C018506:diacetylfluorescein