title: FgNoxR, a regulatory subunit of NADPH oxidases, is required for female fertility and pathogenicity in Fusarium graminearum.
abstract: Fusarium graminearum is a filamentous fungal pathogen that causes wheat Fusarium head blight. In this study, we identified FgNoxR, a regulatory subunit of NADPH oxidases (Nox) in F. graminearum, and found that it plays an important role in the pathogenicity of F. graminearum. FgNoxR is localized on punctate structures throughout the cytoplasm in aerial hyphae while these structures tend to accumulate at or near the plasma membrane, septa and hyphal tips in germinated conidia. Deletion of the FgNOXR gene results in reduced conidiation and germination. Importantly, sexual development is totally abolished in the FgNOXR deletion mutant. In addition, the disease lesion of FgNOXR deletion mutant is limited to the inoculated spikelets of wheat heads. Finally, FgNoxR interacts with FgRac1 and FgNoxA, and all three proteins are required for female fertility. Taken together, our data indicate that FgNoxR contributes to conidiation, sexual reproduction and pathogenesis in F. graminearum.
authors.affiliation: Fujian Province Key Laboratory of Pathogenic Fungi and Mycotoxins, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
authors.forename: Chengkang
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Fujian Province Key Laboratory of Pathogenic Fungi and Mycotoxins, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
authors.forename: Yahong
authors.initials: Y
authors.lastname: Lin
authors.affiliation: Fujian University Key Laboratory for Functional Genomics of Plant Fungal Pathogens, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, China.
authors.forename: Jianqiang
authors.initials: J
authors.lastname: Wang
authors.affiliation: Fujian University Key Laboratory for Functional Genomics of Plant Fungal Pathogens, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Fujian University Key Laboratory for Functional Genomics of Plant Fungal Pathogens, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, China.
authors.forename: Miaoping
authors.initials: M
authors.lastname: Chen
authors.affiliation: Fujian Province Key Laboratory of Pathogenic Fungi and Mycotoxins, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
authors.forename: Justice
authors.initials: J
authors.lastname: Norvienyeku
authors.affiliation: Fujian University Key Laboratory for Functional Genomics of Plant Fungal Pathogens, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, China.
authors.forename: Guangpu
authors.initials: G
authors.lastname: Li
authors.affiliation: Fujian Province Key Laboratory of Pathogenic Fungi and Mycotoxins, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China wenyingyu2004@126.com.
authors.forename: Wenying
authors.initials: W
authors.lastname: Yu
authors.affiliation: Fujian Province Key Laboratory of Pathogenic Fungi and Mycotoxins, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China Fujian University Key Laboratory for Functional Genomics of Plant Fungal Pathogens, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, China.
authors.forename: Zonghua
authors.initials: Z
authors.lastname: Wang
mesh_terms: D005670:Fusarium / Q000235:genetics / Q000254:growth & development / Q000472:pathogenicity*; D017353:Gene Deletion; D005809:Genes, Regulator; D025301:Hyphae / Q000254:growth & development; D008828:Microbiological Techniques; D008853:Microscopy; D009249:NADP / Q000235:genetics / Q000378:metabolism*; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D010935:Plant Diseases / Q000382:microbiology*; D025941:Protein Interaction Mapping; D013172:Spores, Fungal / Q000254:growth & development; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014908:Triticum / Q000382:microbiology; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D014157:Transcription Factors; D037521:Virulence Factors; D009249:NADP; D010088:Oxidoreductases
keywords: Fusarium graminearum; NADPH oxidase; Rac1; pathogenicity; sexual development