title: Development of transcript-associated microsatellite markers in Ancherythoculter nigrocauda and cross-amplification in Culter alburnus.
abstract: Twenty-eight polymorphic microsatellite markers were developed from the transcriptome of Ancherythoculter nigrocauda. These loci were used to characterize the genotypes of 48 individuals. The observed number of alleles per locus ranged from 5 to 11, with an average of 7.7. Expected and observed heterozygosities ranged from 0.437 to 0.978 and from 0.373 to 1.000, respectively. Four of these polymorphic microsatellite loci (HWB14, HWB18, HWB24, and HWB30) deviated significantly from the Hardy-Weinberg equilibrium after use of the sequential Bonferroni correction (P < 0.05). Twenty of the 28 loci could be successfully amplified in Culter alburnus. These novel markers will be useful for germplasm resource conservation and management of A. nigrocauda and C. alburnus.
authors.affiliation: Wuhan Aquaculture Science Research Institute, Wuhan, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Wuhan Aquaculture Science Research Institute, Wuhan, China xfsckj@163.com.
authors.forename: Q
authors.initials: Q
authors.lastname: Li
authors.affiliation: Wuhan Aquaculture Science Research Institute, Wuhan, China.
authors.forename: G
authors.initials: G
authors.lastname: Wang
authors.affiliation: Wuhan Aquaculture Science Research Institute, Wuhan, China.
authors.forename: D
authors.initials: D
authors.lastname: Zhu
authors.affiliation: Wuhan Aquaculture Science Research Institute, Wuhan, China.
authors.forename: J
authors.initials: J
authors.lastname: Chen
authors.affiliation: Wuhan Aquaculture Science Research Institute, Wuhan, China.
authors.forename: P
authors.initials: P
authors.lastname: Li
mesh_terms: D000483:Alleles; D000818:Animals; D003530:Cyprinidae / Q000235:genetics*; D056426:Genetic Loci; D006579:Heterozygote; D018895:Microsatellite Repeats; D011110:Polymorphism, Genetic; D059467:Transcriptome