title: Development and validation of an LC/MS/MS method for simultaneous determination of shionone and epi-friedelinol in rat plasma for pharmacokinetic study after oral administration of Aster tataricus extract.
abstract: A rapid and sensitive liquid chromatography tandem mass spectrometry (LC/MS/MS) method was developed and validated using spinasterol as the internal standard (IS) for the simultaneous determination of shionone and epi-friedelinol in rat plasma. Plasma samples were pretreated using liquid-liquid extraction with ethyl ether. Chromatographic separation was achieved on a C18 column (100 × 2.1 mm, 5 μm) with an isocratic elution consisting of acetonitrile-0.1% formic acid water (75:25, v/v) at a flow rate of 0.30 mL/min. Detection was performed under the selected reaction monitoring scan using an electrospray ionization in the positive ion mode. The mass transitions were as follows: m/z 427.4 → 95.1 for shionone, m/z 411.4 → 205.2 for epi-friedelinol and m/z 395.3 → 105.2 for IS. All calibration curves exhibited good linearity (r > 0.995) over the concentration range for both components. The intra- and inter-day precisions at three QC and lower limit of quantitation levels were both <10.21% in terms of relative standard deviation, and the accuracy ranged from -7.13 to 8.02% in terms of relative error. The extraction recoveries of the compounds ranged from 82.07 to 89.81%. The developed method was successfully applied to the pharmacokinetic study of shionone and epi-friedelinol after oral administration of Aster tataricus extract to rats. Copyright © 2015 John Wiley & Sons, Ltd.
authors.affiliation: Department of Emergency Medicine, Affiliated Hospital of Luzhou Medical College, Luzhou, 646000, China.
authors.forename: De-Feng
authors.initials: DF
authors.lastname: Yin
authors.affiliation: Department of Emergency Medicine, Affiliated Hospital of Luzhou Medical College, Luzhou, 646000, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Zhou
authors.affiliation: Department of Emergency Medicine, Affiliated Hospital of Luzhou Medical College, Luzhou, 646000, China.
authors.forename: Ji-Tao
authors.initials: JT
authors.lastname: Liu
authors.affiliation: Department of Emergency Medicine, Affiliated Hospital of Luzhou Medical College, Luzhou, 646000, China.
authors.forename: Li
authors.initials: L
authors.lastname: Hu
authors.affiliation: Department of Emergency Medicine, Affiliated Hospital of Luzhou Medical College, Luzhou, 646000, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Respiratory Medicine, Affiliated Hospital of Luzhou Medical College, Luzhou, 646000, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Deng
authors.affiliation: Department of Respiratory Medicine, Affiliated Hospital of Luzhou Medical College, Luzhou, 646000, China.
authors.forename: Song-Ping
authors.initials: SP
authors.lastname: Wang
authors.affiliation: Department of Respiratory Medicine, Affiliated Hospital of Luzhou Medical College, Luzhou, 646000, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Xiong
authors.affiliation: Department of Emergency Medicine, Affiliated Hospital of Luzhou Medical College, Luzhou, 646000, China.
authors.forename: Wu
authors.initials: W
authors.lastname: Zhong
mesh_terms: D000284:Administration, Oral; D000818:Animals; D019659:Asteraceae / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods*; D057230:Limit of Detection; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics; D010936:Plant Extracts / Q000008:administration & dosage*; D051381:Rats; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014315:Triterpenes / Q000097:blood* / Q000493:pharmacokinetics
chemical_list: D010936:Plant Extracts; D014315:Triterpenes; C055671:shionone; C055670:friedelinol; D009828:Oleanolic Acid
keywords: aster tataricus extract; epi-friedelinol; pharmacokinetic study; shionone