title: A Novel, Poly(Ethyl Ethylene Ether) Inhibitor to Trypsin from Marine Cyanobacteria, Lyngbya confervoides.
abstract: A novel, poly(ethyl ethylene ether) inhibitor to trypsin was purified from marine cyanobacteria, Lyngbya confervoides from the coastal areas of Thalassery, North Kerala. The kinetics and the thermodynamic parameters of its interactions with the enzyme were also studied. It was demonstrated that the substrate binding, catalytic triad of the enzyme could be blocked by the inhibitor, as expressed by molecular simulation studies. The study also showed that the cyanobacterial group could prove to be a potential source of novel enzyme inhibitors for various applications.
authors.affiliation: Inter University Centre for Bioscience and Department of Biotechnology and Microbiology, Kannur University, Thalassery Campus, Palayad, 670661, India.
authors.forename: Ambika
authors.initials: A
authors.lastname: Devi
authors.affiliation: Inter University Centre for Bioscience and Department of Biotechnology and Microbiology, Kannur University, Thalassery Campus, Palayad, 670661, India.
authors.forename: Shankar
authors.initials: S
authors.lastname: Prasanth
authors.affiliation: Centre for Bioinformatics, Department of Bioinformatics, Bharathiar University, Coimbatore, 641046, India.
authors.forename: Easwaran
authors.initials: E
authors.lastname: Murugesh
authors.affiliation: School of Chemical Sciences, Kannur University, Payannur Campus, Edat, 670327, India.
authors.forename: Karickal R
authors.initials: KR
authors.lastname: Haridas
authors.affiliation: Inter University Centre for Bioscience and Department of Biotechnology and Microbiology, Kannur University, Thalassery Campus, Palayad, 670661, India.
authors.forename: Abdulhameed
authors.initials: A
authors.lastname: Sabu
authors.affiliation: Inter University Centre for Bioscience and Department of Biotechnology and Microbiology, Kannur University, Thalassery Campus, Palayad, 670661, India. mharidasm@rediffmail.com.
authors.forename: Madhathilkovilakathu
authors.initials: M
authors.lastname: Haridas
mesh_terms: D002151:Calorimetry; D002851:Chromatography, High Pressure Liquid; D004987:Ethers; D005030:Ethylenes / Q000737:chemistry / Q000494:pharmacology*; D007700:Kinetics; D062105:Molecular Docking Simulation; D054300:Oscillatoria / Q000201:enzymology*; D012623:Seawater / Q000382:microbiology*; D013055:Spectrophotometry, Infrared; D014357:Trypsin / Q000378:metabolism; D014361:Trypsin Inhibitors / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology
chemical_list: D004987:Ethers; D005030:Ethylenes; D014361:Trypsin Inhibitors; D014357:Trypsin
keywords: Cyanobacteria; Lyngbya sp; Poly(ethyl ethylene ether); Trypsin inhibitors