title: 3,3'-Diindolylmethane: A Promising Sensitizer of γ-Irradiation.
abstract: OBJECTIVE
  Radiotherapy is an effective treatment modality in the clinical treatment of breast cancer. The present work investigated the effect of 3,3'-diindolylmethane (DIM) on γ-irradiation sensitizing human breast carcinoma.
  
  
  METHODS
  Cell survival, intracellular ROS levels, cell cycle distribution, cell apoptosis, and expression of proteins related to apoptosis were measured with MTT assays, flow cytometry, and Western blot analysis, respectively.
  
  
  RESULTS
  In vitro DIM plus γ-irradiation arrested the activity of G2/M phase cell cycle, increased intracellular ROS level, significantly suppressed PARP (poly ADP-ribose polymerase), and enhanced γ-irradiation-induced apoptosis, thereby inhibiting the proliferation of MCF-7 cells.
  
  
  CONCLUSIONS
  These data provide a rationale for the use of DIM as a promising sensitizer of γ-irradiation.
authors.affiliation: Department of Blood Biopharmaceuticals and Viral Detection, Institute of Transfusion Medicine, The Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Blood Biopharmaceuticals and Viral Detection, Institute of Transfusion Medicine, The Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Maomin
authors.initials: M
authors.lastname: Lv
authors.affiliation: Department of Blood Biopharmaceuticals and Viral Detection, Institute of Transfusion Medicine, The Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Chaoji
authors.initials: C
authors.lastname: Huangfu
authors.affiliation: Department of Blood Biopharmaceuticals and Viral Detection, Institute of Transfusion Medicine, The Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.affiliation: Department of Blood Biopharmaceuticals and Viral Detection, Institute of Transfusion Medicine, The Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Jingang
authors.initials: J
authors.lastname: Zhang
mesh_terms: D017209:Apoptosis / Q000187:drug effects* / Q000528:radiation effects*; D002470:Cell Survival / Q000187:drug effects* / Q000528:radiation effects*; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays / Q000627:therapeutic use*; D006801:Humans; D007211:Indoles / Q000008:administration & dosage*; D061986:MCF-7 Cells; D011836:Radiation Tolerance / Q000187:drug effects; D011838:Radiation-Sensitizing Agents / Q000008:administration & dosage; D011878:Radiotherapy / Q000379:methods
chemical_list: D007211:Indoles; D011838:Radiation-Sensitizing Agents; C016392:3,3'-diindolylmethane