title: Agrimonia eupatoria L. and Cynara cardunculus L. Water Infusions: Phenolic Profile and Comparison of Antioxidant Activities.
abstract: Reactive oxygen species (ROS) are highly considered in the ethiopathogenesis of different pathological conditions because they may cause significant damage to cells and tissues. In this paper, we focused on potential antioxidant properties of two medical plants such as the Agrimonia eupatoria L. and Cynara cardunculus L. Both plants have previously been studied for their pharmacological activities, especially as hepatoprotective and hypoglycemic activities. It has been suggested, that their effects are related to the antioxidant properties of polyphenols, which are dominant compounds of the plants' extracts. In the present study HPLC-MS analysis of water infusion was performed allowing the identification of several phenolic constituents. Furthermore, antioxidant effects of the two extracts were compared showing higher effects for agrimony extract compared to artichoke. Thus, agrimony was selected for the in vivo study using the skin flap viability model. In conclusion, our results provide evidence that the A. eupatoria extract may be a valuable source of polyphenols to be studied for the future development of supplements useful in the prevention of diseases linked to oxidative stress.
authors.affiliation: Department of Pharmacognosy and Botany, Faculty of Pharmacy, Comenius University, Odbojárov 10, 832 32 Bratislava, Slovakia. kuczmannova@fpharm.uniba.com.
authors.forename: Anika
authors.initials: A
authors.lastname: Kuczmannová
authors.affiliation: Department of Pharmacognosy and Botany, Faculty of Pharmacy, Comenius University, Odbojárov 10, 832 32 Bratislava, Slovakia. mucaji@fpharm.uniba.sk.|Department of Pharmacology, Faculty of Medicine, Pavol Jozef Šafárik University, Trieda SNP 1, 040 11 Košice, Slovakia. mucaji@fpharm.uniba.sk.|Department for Biomedical Research, East-Slovak Institute of Cardiovascular Diseases, Inc., Ondavská 8, 040 11 Košice, Slovakia. mucaji@fpharm.uniba.sk.|Institute of Anatomy, 1st Faculty of Medicine, Charles University, U nemocnice 2, 128 00 Prague, Czech Republic. mucaji@fpharm.uniba.sk.
authors.forename: Peter
authors.initials: P
authors.lastname: Gál
authors.affiliation: Department of Pharmacology, Faculty of Medicine, Pavol Jozef Šafárik University, Trieda SNP 1, 040 11 Košice, Slovakia. lenka.varinska@upjs.sk.|Department for Biomedical Research, East-Slovak Institute of Cardiovascular Diseases, Inc., Ondavská 8, 040 11 Košice, Slovakia. lenka.varinska@upjs.sk.
authors.forename: Lenka
authors.initials: L
authors.lastname: Varinská
authors.affiliation: Department of Molecular Biology and Pharmaceutical Biotechnology, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences, Palackého 1-3, 612 42 Brno, Czech Republic. tremlj@vfu.cz.
authors.forename: Jakub
authors.initials: J
authors.lastname: Treml
authors.affiliation: Department for Biomedical Research, East-Slovak Institute of Cardiovascular Diseases, Inc., Ondavská 8, 040 11 Košice, Slovakia. ivankovac.kovi@gmail.com.|2nd Department of Surgery, Pavol Jozef Šafárik University and Louise Pasteur University Hospital, 041 90 Košice, Slovakia. ivankovac.kovi@gmail.com.
authors.forename: Ivan
authors.initials: I
authors.lastname: Kováč
authors.affiliation: Department for Biomedical Research, East-Slovak Institute of Cardiovascular Diseases, Inc., Ondavská 8, 040 11 Košice, Slovakia. martin.novotny.dr@gmail.com.|Department of Infectology and Travel Medicine, Pavol Jozef Šafárik University and Louise Pasteur University Hospital, 041 90 Košice, Slovakia. martin.novotny.dr@gmail.com.
authors.forename: Martin
authors.initials: M
authors.lastname: Novotný
authors.affiliation: Department for Biomedical Research, East-Slovak Institute of Cardiovascular Diseases, Inc., Ondavská 8, 040 11 Košice, Slovakia. tomasvasilenko@gmail.com.|Department of Surgery, Pavol Jozef Šafárik University and Košice-Šaca Hospital, 040 15 Košice-Šaca, Slovakia. tomasvasilenko@gmail.com.
authors.forename: Tomáš
authors.initials: T
authors.lastname: Vasilenko
authors.affiliation: Department of Pharmaceutical and Pharmacological Sciences, University of Padova, Via F. Marzolo 5, 351 31 Padova, Italy. stefano.dallacqua@unipd.it.
authors.forename: Stefano
authors.initials: S
authors.lastname: Dall'Acqua
authors.affiliation: Department of Pharmacognosy and Botany, Faculty of Pharmacy, Comenius University, Odbojárov 10, 832 32 Bratislava, Slovakia. nagy@fpharm.uniba.sk.
authors.forename: Milan
authors.initials: M
authors.lastname: Nagy
authors.affiliation: Department of Pharmacognosy and Botany, Faculty of Pharmacy, Comenius University, Odbojárov 10, 832 32 Bratislava, Slovakia. pgal@vusch.sk.
authors.forename: Pavel
authors.initials: P
authors.lastname: Mučaji
mesh_terms: D031981:Agrimonia / Q000737:chemistry*; D000818:Animals; D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology; D002374:Catalase / Q000378:metabolism; D002460:Cell Line / Q000187:drug effects / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D031942:Cynara / Q000737:chemistry*; D004249:DNA Damage / Q000187:drug effects; D006801:Humans; D018384:Oxidative Stress / Q000187:drug effects; D010636:Phenols / Q000737:chemistry* / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D010636:Phenols; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D002374:Catalase; D013482:Superoxide Dismutase
keywords: ROS; agrimony; antioxidants; artichoke; oxidative stress