title: Label-free assessment of replicative senescence in mesenchymal stem cells by Raman microspectroscopy.
abstract: Here, Raman microspectroscopy was employed to assess replicative senescence of mesenchymal stem cells (MSC). A regular spectral change related to the cell senescence was found in the ratio of two peaks at 1157 cm(-1) and 1174 cm(-1), which are assigned to C-C, C-N stretching vibrations in proteins and C-H bending vibrations in tyrosine and phenylalanine, respectively. With the cell aging, the ratio I1157 / I1174 exhibited a monotonic decline and showed small standard deviations, so that it can statistically distinguish between cells having slight changes in terms of aging. We propose that I1157 / I1174 can act as a characteristic spectral signature for label-free assessment of MSC senescence.
authors.affiliation: College of Electronic and Information Engineering, Tianjin Polytechnic University, Tianjin 300387, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Bai
authors.affiliation: College of Electronic and Information Engineering, Tianjin Polytechnic University, Tianjin 300387, China.
authors.forename: Haiyu
authors.initials: H
authors.lastname: Li
authors.affiliation: State Key Laboratory of Experimental Hematology, Institute of Hematology and Hospital of Blood Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300020, China ; zhibohan@163.com.
authors.forename: Zhibo
authors.initials: Z
authors.lastname: Han
authors.affiliation: College of Electronic and Information Engineering, Tianjin Polytechnic University, Tianjin 300387, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Zhang
authors.affiliation: College of Electronic and Information Engineering, Tianjin Polytechnic University, Tianjin 300387, China.
authors.forename: Junfa
authors.initials: J
authors.lastname: Zhao
authors.affiliation: College of Electronic and Information Engineering, Tianjin Polytechnic University, Tianjin 300387, China ; miaochangyun@tjpu.edu.cn.
authors.forename: Changyun
authors.initials: C
authors.lastname: Miao
authors.affiliation: National Engineering Research Center of Cell Products / AmCellGene Co. Ltd, Tianjin 300457, China.
authors.forename: Shulin
authors.initials: S
authors.lastname: Yan
authors.affiliation: National Engineering Research Center of Cell Products / AmCellGene Co. Ltd, Tianjin 300457, China.
authors.forename: Aibin
authors.initials: A
authors.lastname: Mao
authors.affiliation: Tianjin Key Laboratory of Food and Biotechnology, School of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Experimental Hematology, Institute of Hematology and Hospital of Blood Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300020, China ; National Engineering Research Center of Cell Products / AmCellGene Co. Ltd, Tianjin 300457, China ; hanzhongchao@hotmail.com.
authors.forename: Zhongchao
authors.initials: Z
authors.lastname: Han
keywords: (170.0170) Medical optics and biotechnology; (170.5660) Raman spectroscopy