title: Proteomic research on diapause-related proteins in the female ladybird, Coccinella septempunctata L.
abstract: In the experiments reported here, we used the female ladybird Coccinella septempunctata L. as a model to identify diapause-associated proteins using proteomics technology. Our results indicated that protein expression patterns of diapausing and nondiapausing individuals were highly differentiated. A total of 58 spots showed significant differences in abundance (Ratio > 2 and P < 0.05) according to two-dimensional electrophoresis and GE Image Scanner III analysis. Sixteen protein spots were further investigated using mass spectrometry. Eight proteins were characterized, including chaperones and proteins involved in glucose metabolism, lipid metabolism, and the tricarboxylic acid cycle. Among these proteins, five proteins were upregulated in diapausing female adults, including a chaperone (Symbionin symL), malate dehydrogenase (putative), two proteins linked to lipid metabolism (unknown and conserved hypothetical protein) and phosphoglyceromutase (partial). By contrast, isocitrate dehydrogenase (RH49423p), fumarylacetoacetate hydrolase (AGAP001942-PA), and a putative medium chain acyl-CoA dehydrogenase were downregulated. These results contribute to the understanding of diapause mechanisms of the ladybird C. septempunctata and may suggest methods for improving the application of this natural enemy insect.
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences;Sino-American Biological Control Laboratory,USDA-ARS,Beijing 100081,P.R. China.
authors.forename: X Y
authors.initials: XY
authors.lastname: Ren
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences;Sino-American Biological Control Laboratory,USDA-ARS,Beijing 100081,P.R. China.
authors.forename: L S
authors.initials: LS
authors.lastname: Zhang
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences;Sino-American Biological Control Laboratory,USDA-ARS,Beijing 100081,P.R. China.
authors.forename: Y H
authors.initials: YH
authors.lastname: Han
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences;Sino-American Biological Control Laboratory,USDA-ARS,Beijing 100081,P.R. China.
authors.forename: T
authors.initials: T
authors.lastname: An
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences;Sino-American Biological Control Laboratory,USDA-ARS,Beijing 100081,P.R. China.
authors.forename: Y
authors.initials: Y
authors.lastname: Liu
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences;Sino-American Biological Control Laboratory,USDA-ARS,Beijing 100081,P.R. China.
authors.forename: Y Y
authors.initials: YY
authors.lastname: Li
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences;Sino-American Biological Control Laboratory,USDA-ARS,Beijing 100081,P.R. China.
authors.forename: H Y
authors.initials: HY
authors.lastname: Chen
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D002952:Citric Acid Cycle; D001517:Coleoptera / Q000737:chemistry* / Q000502:physiology; D064666:Diapause, Insect / Q000502:physiology; D015536:Down-Regulation; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D005947:Glucose / Q000378:metabolism; D006867:Hydrolases / Q000378:metabolism; D007091:Image Processing, Computer-Assisted; D019476:Insect Proteins / Q000378:metabolism*; D007521:Isocitrate Dehydrogenase / Q000378:metabolism; D050356:Lipid Metabolism; D008291:Malate Dehydrogenase / Q000378:metabolism; D018832:Molecular Chaperones / Q000378:metabolism; D040901:Proteomics; D021241:Spectrometry, Mass, Electrospray Ionization; D015854:Up-Regulation
chemical_list: D019476:Insect Proteins; D018832:Molecular Chaperones; D008291:Malate Dehydrogenase; D007521:Isocitrate Dehydrogenase; D006867:Hydrolases; C020156:fumarylacetoacetase; D005947:Glucose
keywords: Coccinella septempunctata; diapause associated proteins; female adults; two-dimensional electrophoresis