title: Reversed-phase ion-pair high-performance liquid chromatography assay of polyprenyl diphosphate oligomer homologues.
abstract: A reversed-phase ion-pair high-performance liquid chromatography procedure was developed for the separation of polyprenyl diphosphate oligomer homologues obtained chemically from plant polyprenols. Tetrabutylammonium phosphate was used as the ion-pair reagent, and the dependence of the separation quality on pH of ion-pair reagent was investigated for the first time. The procedure is applicable for the control of commercial available polyprenyl monophosphates (the active components of veterinary drugs Phosprenyl and Gamapren) for the possible presence of polyprenyl diphosphate byproducts.
authors.affiliation: Laboratory of natural immunity, Department of immunology, N.F. Gamaleya Federal Research Centre of Epidemiology and Microbiology, Moscow, Russian Federation.
authors.forename: Vyacheslav V
authors.initials: VV
authors.lastname: Kozlov
authors.affiliation: Laboratory of Carbohydrate Chemistry, N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russian Federation.
authors.forename: Leonid L
authors.initials: LL
authors.lastname: Danilov
mesh_terms: D001704:Biopolymers / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D045782:Hemiterpenes; D000439:Pentanols / Q000032:analysis*
chemical_list: D001704:Biopolymers; D045782:Hemiterpenes; D000439:Pentanols; C009034:prenol
keywords: Polyprenyl diphosphates; Reversed-phase high-performance liquid chromatography; Tetrabutylammonium phosphate