title: Optimization of the Biosynthesis Conditions of Daptomycin by the Biostatistical Methodology.
abstract: Response surface methodology (RSM) was employed to optimize medium components including oxygen vector of n-dodecane of a mutant strain GC-63 of Streptomyces roseosporus NRRL 11379. The two-level Plackett-Burman design (PB factorial design) with fourteen variables including oxygen vector was used to screen the most significant factors affecting antibiotic production. Then, the RSM based on center composite design was used to identify the optimum levels of the significant variables to generate optimal response. Glucose, soybean meal, asparagine and n-dodecane were screened to significantly influence the daptomycin production. The medium composition optimized with response surface methodology was (g/L): glucose, 9.46; soluble starch, 25; dextrin, 12.5; yeast extract, 12.5; soybean meal, 21.34; peptone, 25; casein, 5; asparagine, 2.68; K2SO4, 6; (NH4)2Fe(SO4)2, 2; MgSO4, 1; CaCO3, 5; MnCl2, 0.5; n-dodecane, 7.47 % (v/v). The maximum daptomycin concentration reached 979.36 mg/L which was nearly 2.2-fold higher compared to that in the basal medium, with predicted optimal concentrations in a 7.5-L fermentor.
authors.affiliation: Department of Bio-Engineering, College of Biological Engineering, Henan University of Technology, Zhengzhou, 450001, People's Republic of China. yuguanghai115@126.com.
authors.forename: Guanghai
authors.initials: G
authors.lastname: Yu
authors.affiliation: College of Environmental Science and Engineering, Taiyuan University of Technology, Taiyuan, 030024, People's Republic of China.
authors.forename: Guoying
authors.initials: G
authors.lastname: Wang
mesh_terms: D000900:Anti-Bacterial Agents / Q000096:biosynthesis; D056808:Biostatistics / Q000379:methods*; D017576:Daptomycin / Q000096:biosynthesis*; D013302:Streptomyces / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D017576:Daptomycin
keywords: Biostatistical analysis; Daptomycin; Optimization; Oxygen vector; Response surface methodology; Streptomyces roseosporus; n-Dodecane