title: Rapid imaging of surgical breast excisions using direct temporal sampling two photon fluorescent lifetime imaging.
abstract: Two photon fluorescent lifetime imaging is a modality that enables depth-sectioned, molecularly-specific imaging of cells and tissue using intrinsic contrast. However, clinical applications have not been well explored due to low imaging speed and limited field of view, which make evaluating large pathology samples extremely challenging. To address these limitations, we have developed direct temporal sampling two photon fluorescent lifetime imaging (DTS-FLIM), a method which enables a several order of magnitude increase in imaging speed by capturing an entire lifetime decay in a single fluorescent excitation. We use this greatly increased speed to perform a preliminary study using gigapixel-scale imaging of human breast pathology surgical specimens.
authors.affiliation: Department of Electrical Engineering and Computer Science and Research Laboratory of Electronics, Massachusetts Institute of Technology, Cambridge, MA, USA.
authors.forename: Michael G
authors.initials: MG
authors.lastname: Giacomelli
authors.affiliation: Department of Pathology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA ; Alternative spelling of this author's name is Yury Sheykin.
authors.forename: Yuri
authors.initials: Y
authors.lastname: Sheikine
authors.affiliation: Department of Pathology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.
authors.forename: Hilde
authors.initials: H
authors.lastname: Vardeh
authors.affiliation: Department of Pathology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.
authors.forename: James L
authors.initials: JL
authors.lastname: Connolly
authors.affiliation: Department of Electrical Engineering and Computer Science and Research Laboratory of Electronics, Massachusetts Institute of Technology, Cambridge, MA, USA.
authors.forename: James G
authors.initials: JG
authors.lastname: Fujimoto
keywords: (170.2520) Fluorescence microscopy; (170.3880) Medical and biological imaging