title: Identification and Characterization of the Trypanosoma cruzi B-cell Superantigen Tc24.
abstract: Trypanosoma cruzi causes life-long disease after infection and leads to cardiac disease in 30% of infected individuals. After infection, the parasites are readily detectable in the blood during the first few days before disseminating to infect numerous cell types. Preliminary data suggested that the Tc24 protein that localizes to the T. cruzi membrane during all life stages possesses B-cell superantigenic properties. These antigens facilitate immune escape by interfering with antibody-mediated responses, particularly the avoidance of catalytic antibodies. These antibodies are an innate host defense mechanism present in the naive repertoire, and catalytic antibody-antigen binding results in hydrolysis of the target. We tested the B-cell superantigenic properties of Tc24 by comparing the degree of Tc24 hydrolysis by IgM purified from either Tc24 unexposed or exposed mice and humans. Respective samples were subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis, silver stained, and the degree of hydrolysis was measured. Data presented in this report suggest that the T. cruzi Tc24 is a B-cell superantigen based on the observations that 1) Tc24 was hydrolyzed by IgM present in serum of unexposed mice and humans and 2) exposure to Tc24 eliminated catalytic activity as early as 4 days after T. cruzi infection.
authors.forename: Sarah M
authors.initials: SM
authors.lastname: Gunter
authors.forename: Kathryn M
authors.initials: KM
authors.lastname: Jones
authors.forename: Bin
authors.initials: B
authors.lastname: Zhan
authors.forename: Heather T
authors.initials: HT
authors.lastname: Essigmann
authors.forename: Kristy O
authors.initials: KO
authors.lastname: Murray
authors.forename: Melissa N
authors.initials: MN
authors.lastname: Garcia
authors.forename: Rodion
authors.initials: R
authors.lastname: Gorchakov
authors.forename: Maria Elena
authors.initials: ME
authors.lastname: Bottazzi
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Hotez
authors.forename: Eric L
authors.initials: EL
authors.lastname: Brown
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D000953:Antigens, Protozoan / Q000235:genetics / Q000378:metabolism*; D016022:Case-Control Studies; D014355:Chagas Disease / Q000276:immunology; D005786:Gene Expression Regulation; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood; D051379:Mice; D011994:Recombinant Proteins; D018089:Superantigens / Q000235:genetics / Q000378:metabolism*; D014349:Trypanosoma cruzi / Q000378:metabolism*
chemical_list: D000953:Antigens, Protozoan; D007075:Immunoglobulin M; D011994:Recombinant Proteins; D018089:Superantigens