title: A Series of Imidazole Derivatives: Synthesis, Two-Photon Absorption, and Application for Bioimaging.
abstract: A new series of D-π-A type imidazole derivatives have been synthesized and characterized. Two corresponding imidazolium salts (iodine and hexafluorophosphate) were prepared from the imidazole compound. Their electron-withdrawing ability can be largely tunable by salt formation reaction or ion exchange. UV-vis absorption and single-photon fluorescence spectra have been systematically investigated in different solvents. The two-photon cross sections (δ 2PA) of the imidazole derivatives are measured by two-photon excited fluorescence (2PEF) method. Compared with those of T-1 (107 GM) and T-3 (96 GM), T-2 (imidazolium iodine salt) has a large two-photon absorption (2PA) cross section value of 276 GM. Furthermore, the cytotoxicity and applications in bioimaging for the imidazole derivatives were carried out. The results showed that T-1 can be used as a lysosomal tracker with high stability and water solubility within pHs of 4-6, while T-2 and T-3 can be used as probes for cell cytoplasm.
authors.affiliation: Department of Chemistry, Key Laboratory of Functional Inorganic Material Chemistry of Anhui Province, Anhui University, Hefei 230601, China.
authors.forename: Yingzhong
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Department of Chemistry, Key Laboratory of Functional Inorganic Material Chemistry of Anhui Province, Anhui University, Hefei 230601, China ; Department of Food and Environmental Engineering, Chuzhou Vocational and Technical College, Chuzhou 239000, China.
authors.forename: Lufei
authors.initials: L
authors.lastname: Xiao
authors.affiliation: Department of Chemistry, Key Laboratory of Functional Inorganic Material Chemistry of Anhui Province, Anhui University, Hefei 230601, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Zhao
authors.affiliation: Department of Chemistry, Key Laboratory of Functional Inorganic Material Chemistry of Anhui Province, Anhui University, Hefei 230601, China.
authors.forename: Jiazheng
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Department of Chemistry, Key Laboratory of Functional Inorganic Material Chemistry of Anhui Province, Anhui University, Hefei 230601, China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Department of Chemistry, Key Laboratory of Functional Inorganic Material Chemistry of Anhui Province, Anhui University, Hefei 230601, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Chemistry, Key Laboratory of Functional Inorganic Material Chemistry of Anhui Province, Anhui University, Hefei 230601, China.
authors.forename: Shengli
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Chemistry, Key Laboratory of Functional Inorganic Material Chemistry of Anhui Province, Anhui University, Hefei 230601, China.
authors.forename: Hongping
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Department of Chemistry, Key Laboratory of Functional Inorganic Material Chemistry of Anhui Province, Anhui University, Hefei 230601, China.
authors.forename: Jieying
authors.initials: J
authors.lastname: Wu
authors.affiliation: Department of Chemistry, Key Laboratory of Functional Inorganic Material Chemistry of Anhui Province, Anhui University, Hefei 230601, China ; State Key Laboratory of Coordination Chemistry, Nanjing University, Nanjing 210093, China.
authors.forename: Yupeng
authors.initials: Y
authors.lastname: Tian
mesh_terms: D065638:Absorption, Radiation; D003287:Contrast Media / Q000138:chemical synthesis*; D056945:Hep G2 Cells; D006801:Humans; D007089:Image Enhancement / Q000379:methods*; D007093:Imidazoles / Q000138:chemical synthesis*; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D017785:Photons; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013347:Subcellular Fractions / Q000648:ultrastructure*
chemical_list: D003287:Contrast Media; D007093:Imidazoles; C029899:imidazole