title: Genome-wide RNA-Seq analysis of breast muscles of two broiler chicken groups differing in shear force.
abstract: In this study, a whole transcriptome analysis of breast muscles was conducted in broiler chicken groups differing in shear force. Shear force is a determinant of tenderness, which in turn is one of the most important parameters of meat quality in chickens. In our analysis, a total of 11,560 transcripts and 9824 genes per sample were identified. In chickens with more tender meat, up-regulation of 19 genes and down-regulation of 49 genes was observed. The up-regulated gene group included the ASB2 gene, which is probably involved in the meat conversion process, as its product results in the degradation of filamins, proteins which form muscle fibres. In the down-regulated gene group, genes which play a role in lipogenesis (THRSP, PLIN1) and in collagen synthesis (P4HA3, LEPREL4, PCOLCE2, COL16A1, COL20A1, VWA1) were detected. Their presence suggests the involvement of the extracellular matrix in the determination of meat tenderness. Thus, our study identified a pool of genes that may participate in the tenderisation process in broiler chickens.
authors.affiliation: Department of Animal Genomics and Molecular Biology, National Research Institute of Animal Production, 32-083 Balice, Poland.
authors.forename: K
authors.initials: K
authors.lastname: Piórkowska
authors.affiliation: Department of Animal Genetics and Breeding, National Research Institute of Animal Production, 32-083 Balice, Poland.
authors.forename: K
authors.initials: K
authors.lastname: Żukowski
authors.affiliation: Department of Animal Genetics and Breeding, National Research Institute of Animal Production, 32-083 Balice, Poland.
authors.forename: J
authors.initials: J
authors.lastname: Nowak
authors.affiliation: Department of Animal Genetics and Breeding, National Research Institute of Animal Production, 32-083 Balice, Poland.
authors.forename: K
authors.initials: K
authors.lastname: Połtowicz
authors.affiliation: Department of Animal Genomics and Molecular Biology, National Research Institute of Animal Production, 32-083 Balice, Poland.
authors.forename: K
authors.initials: K
authors.lastname: Ropka-Molik
authors.affiliation: Department of Animal Genomics and Molecular Biology, National Research Institute of Animal Production, 32-083 Balice, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Gurgul
mesh_terms: D000818:Animals; D002645:Chickens / Q000235:genetics*; D003094:Collagen / Q000096:biosynthesis; D005109:Extracellular Matrix / Q000235:genetics; D064448:Filamins / Q000235:genetics; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D050155:Lipogenesis / Q000235:genetics; D008460:Meat; D008969:Molecular Sequence Data; D009132:Muscles / Q000378:metabolism
chemical_list: D064448:Filamins; D003094:Collagen
keywords: differentially expressed gene; poultry; tenderness; transcriptome analysis