title: A simple and rapid creatinine sensing via DLS selectivity, using calix[4]arene thiol functionalized gold nanoparticles.
abstract: A new, simple, ultra-sensitive and selective approach has been reported for the "on spot" colorimetric detection of creatinine based on calix[4]arene functionalized gold nanoparticles (AuNPs) with excellent discrimination in the presence of other biomolecules. The lower detection limit of the method is 2.16nM. The gold nanoparticles and p-tert-butylcalix[4]arene were synthesized by microwave assisted method. Specifically, in our study, we used dynamic light scattering (DLS) which is a powerful method for the determination of small changes in particle size, improved selectivity and sensitivity of the creatinine detection system over colorimetric method. The nanoassembly is characterized by Transmission electron microscopy (TEM), DLS, UV-vis and ESI-MS spectroscopy, which demonstrates the binding affinity due its ability of hydrogen bonding and electrostatic interaction between -NH group of creatinine and pSDSC4. It exhibits fast response time (<60s) to creatinine and has long shelf-life (>5 weeks). The developed pSDSC4-AuNPs based creatinine biosensor will be established as simple, reliable and accurate tool for the determination of creatinine in human urine samples.
authors.affiliation: Bhavans Shree I. L. Pandya Arts, Science College, Gujarat University, Dakor 388225, Gujarat, India. Electronic address: pinkeshsutariya@gmail.com.
authors.forename: Pinkesh G
authors.initials: PG
authors.lastname: Sutariya
authors.affiliation: Institute of Life Sciences, School of Science and Technology, Ahmedabad University, Ahmedabad, Gujarat 380009, India. Electronic address: alokpandya20@gmail.com.
authors.forename: Alok
authors.initials: A
authors.lastname: Pandya
authors.affiliation: Department of Forensic Science, School of Sciences, Gujarat University, Ahmedabad, Gujarat 380009, India. Electronic address: forensicwithanand@gmail.com.
authors.forename: Anand
authors.initials: A
authors.lastname: Lodha
authors.affiliation: Department of Forensic Science, School of Sciences, Gujarat University, Ahmedabad, Gujarat 380009, India. Electronic address: shobhanamenon07@gmail.com.
authors.forename: Shobhana K
authors.initials: SK
authors.lastname: Menon
mesh_terms: D015374:Biosensing Techniques; D047250:Calixarenes / Q000737:chemistry*; D003124:Colorimetry / Q000379:methods; D003404:Creatinine / Q000737:chemistry / Q000652:urine*; D006046:Gold / Q000737:chemistry*; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D021241:Spectrometry, Mass, Electrospray Ionization; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D013438:Sulfhydryl Compounds / Q000737:chemistry*
chemical_list: D013438:Sulfhydryl Compounds; D047250:Calixarenes; D006046:Gold; D003404:Creatinine
keywords: Biosensor; Calix[4]arene; Creatinine; DLS selectivity; Gold nanoparticles