title: Influence of Phytase Transgenic Corn on the Intestinal Microflora and the Fate of Transgenic DNA and Protein in Digesta and Tissues of Broilers.
abstract: An experiment was conducted to investigate the effect of phytase transgenic corn (PTC) on intestinal microflora, and the fate of transgenic DNA and protein in the digesta and tissues of broilers. A total of 160 1-day-old Arbor Acres commercial male broilers were randomly assigned to 20 cages (8 chicks per cage) with 10 cages (replicates) for each treatment. Birds were fed with a diet containing either PTC (54.0% during 1-21 days and 61.0% during 22-42 days) or non-transgenic isogenic control corn (CC) for a duration of 42 days. There were no significant differences (P>0.05) between birds fed with the PTC diets and those fed with the CC diets in the quantities of aerobic bacteria, anaerobic bacteria, colibacillus and lactobacilli, or microbial diversities in the contents of ileum and cecum. Transgenic phyA2 DNA was not detected, but phyA2 protein was detected in the digesta of duodenum and jejunum of broilers fed with the PTC diets. Both transgenic phyA2 DNA and protein fragments were not found in the digesta of the ileum and rectum, heart, liver, kidney, and breast or thigh muscles of broilers fed with the PTC diets. It was concluded that PTC had no adverse effect on the quantity and diversity of gut microorganisms; Transgenic phyA2 DNA or protein was rapidly degraded in the intestinal tract and was not transferred to the tissues of broilers.
authors.affiliation: Mineral Nutrition Research Division, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Lu
authors.affiliation: Mineral Nutrition Research Division, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.|College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou, China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Guo
authors.affiliation: Department of Animal Science, Hebei Normal University of Science and Technology, Qinhuangdao, China.
authors.forename: Sufen
authors.initials: S
authors.lastname: Li
authors.affiliation: College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou, China.
authors.forename: Ang
authors.initials: A
authors.lastname: Li
authors.affiliation: Mineral Nutrition Research Division, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Liyang
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Nutrition, School of Public Health and Health Sciences, University of Massachusetts, Amherst, Massachusetts, United States of America.
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Mineral Nutrition Research Division, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Xugang
authors.initials: X
authors.lastname: Luo
mesh_terms: D010832:6-Phytase / Q000235:genetics*; D000818:Animals; D044822:Biodiversity; D015153:Blotting, Western; D002645:Chickens; D018744:DNA, Plant / Q000378:metabolism*; D058645:Denaturing Gradient Gel Electrophoresis; D000069196:Gastrointestinal Microbiome; D007413:Intestinal Mucosa / Q000378:metabolism; D008297:Male; D009928:Organ Specificity; D030821:Plants, Genetically Modified; D016133:Polymerase Chain Reaction; D003313:Zea mays / Q000235:genetics*
chemical_list: D018744:DNA, Plant; D010832:6-Phytase