title: Synthesis of Non-Natural Heteroaminopolysaccharides by α-Glucan Phosphorylase-Catalyzed Enzymatic Copolymerization: α(1→4)-Linked Glucosaminoglucans.
abstract: Thermostable α-glucan phosphorylase-catalyzed enzymatic copolymerization of α-d-glucose 1-phosphate (Glc-1-P) with its analogue monomer, α-d-glucosamine 1-phosphate (GlcN-1-P), from a maltotriose primer was performed to produce non-natural heteroaminopolysaccharides composed of Glc/GlcN units, that is, α(1→4)-linked glucosaminoglucans. The GlcN units in the products were further converted to N-acetyl-d-glucosamine (GlcNAc) units by N-acetylation. The structures of the products were evaluated by the MALDI-TOF MS, (1)H NMR, and (1)H-(1)H COSY NMR measurements, which were completely different from those of the natural glycosaminoglycans. The degrees of polymerization and Glc/GlcN compositional ratios of the products were relatively dependent on the Glc-1-P/Glc-1-P/Glc3 feed ratios. The noncrystalline natures of the present materials were supported by the X-ray diffraction measurement.
authors.affiliation: Department of Chemistry, Biotechnology, and Chemical Engineering, Graduate School of Science and Engineering, Kagoshima University , 1-21-40 Korimoto, Kagoshima 890-0065, Japan.
authors.forename: Kento
authors.initials: K
authors.lastname: Yamashita
authors.affiliation: Department of Chemistry, Biotechnology, and Chemical Engineering, Graduate School of Science and Engineering, Kagoshima University , 1-21-40 Korimoto, Kagoshima 890-0065, Japan.
authors.forename: Kazuya
authors.initials: K
authors.lastname: Yamamoto
authors.affiliation: Department of Chemistry, Biotechnology, and Chemical Engineering, Graduate School of Science and Engineering, Kagoshima University , 1-21-40 Korimoto, Kagoshima 890-0065, Japan.
authors.forename: Jun-ichi
authors.initials: J
authors.lastname: Kadokawa
mesh_terms: D000107:Acetylation; D000117:Acetylglucosamine / Q000737:chemistry; D001426:Bacterial Proteins / Q000737:chemistry* / Q000302:isolation & purification; D055162:Biocatalysis; D002240:Carbohydrate Sequence; D004795:Enzyme Stability; D005944:Glucosamine / Q000031:analogs & derivatives / Q000737:chemistry; D005958:Glucosephosphates / Q000737:chemistry; D006025:Glycosaminoglycans / Q000737:chemistry*; D006358:Hot Temperature; D008969:Molecular Sequence Data; D006005:Phosphorylases / Q000737:chemistry* / Q000302:isolation & purification; D058105:Polymerization; D011134:Polysaccharides / Q000138:chemical synthesis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D001426:Bacterial Proteins; D005958:Glucosephosphates; D006025:Glycosaminoglycans; D011134:Polysaccharides; C020341:glucosaminoglycans; C038515:glucosamine 1-phosphate; C031590:glucose-1-phosphate; D006005:Phosphorylases; D005944:Glucosamine; D000117:Acetylglucosamine