title: Pseudorabies virus isolates from domestic pigs and wild boars show no apparent in vitro differences in replication kinetics and sensitivity to interferon-induced antiviral status.
abstract: Pseudorabies virus is the causative agent of Aujeszky's disease. Domestic pigs and wild boars are its natural hosts, and strains circulating within both populations differ in their capacity to induce clinical disease. Cell biological and molecular explanations for the observed differences in virulence are, however, lacking. Different virulence determinants that can be assessed in vitro were determined for five domestic swine strains, four wild boar strains and the NIA3 reference strain. Replication kinetics and plaque formation capacity in continuous swine testicular cells and different primary porcine cell lines were highly similar for isolates from both populations. Treatment of these cell lines with IFNα, IFNγ or a combination of both provoked similar plaque-reducing effects for all strains. In conclusion, our results indicate that isolates from domestic swine and wild boar differ neither in intrinsic replication and dissemination capacity nor in sensitivity to antiviral effects of IFNs.
authors.affiliation: Enzootic and (Re)emerging Diseases, Operational Direction Viral Diseases, CODA-CERVA, Groeselenberg 99, 1180 Ukkel, Belgium.
authors.forename: Sara
authors.initials: S
authors.lastname: Verpoest
authors.affiliation: Enzootic and (Re)emerging Diseases, Operational Direction Viral Diseases, CODA-CERVA, Groeselenberg 99, 1180 Ukkel, Belgium.
authors.forename: Ann Brigitte
authors.initials: AB
authors.lastname: Cay
authors.affiliation: Department of Virology, Immunology and Parasitology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium.
authors.forename: Herman
authors.initials: H
authors.lastname: Favoreel
authors.affiliation: Enzootic and (Re)emerging Diseases, Operational Direction Viral Diseases, CODA-CERVA, Groeselenberg 99, 1180 Ukkel, Belgium.|Department of Virology, Immunology and Parasitology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium.
authors.forename: Nick
authors.initials: N
authors.lastname: De Regge
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000378:metabolism*; D002478:Cells, Cultured; D011558:Herpesvirus 1, Suid / Q000276:immunology* / Q000302:isolation & purification / Q000502:physiology*; D007372:Interferons / Q000378:metabolism*; D034421:Sus scrofa; D010948:Viral Plaque Assay; D014779:Virus Replication
chemical_list: D000998:Antiviral Agents; D007372:Interferons