title: Comparison of the Efficiency of Viral Transduction and Episomal Transfection in Human Fibroblast Reprogramming.
abstract: Induced pluripotent cells were derived from adult human skin fibroblast by using two methods of reprogramming. Episomal transfection with vectors containing oriP/EBNA-1 sequence for delivery of reprogramming genes Oct4, Sox2, Klf4, L-Myc, and Lin28 proved to be more effective than viral transduction with Sendai virus-based vector: ~200 and 8 colonies per 10(5) cells were found on day 21 of culturing, respectively. Colonies of induced pluripotent cells obtained by these two methods expressed pluripotency marker Tra1-60.
authors.affiliation: V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia.
authors.forename: A S
authors.initials: AS
authors.lastname: Vdovin
authors.affiliation: V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia.
authors.forename: A Yu
authors.initials: AY
authors.lastname: Lupatov
authors.affiliation: V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia. irkhol@yandex.ru.
authors.forename: I V
authors.initials: IV
authors.lastname: Kholodenko
authors.affiliation: V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia.
authors.forename: K N
authors.initials: KN
authors.lastname: Yarygin
mesh_terms: D050156:Adipogenesis; D002478:Cells, Cultured; D065150:Cellular Reprogramming; D000067470:Cellular Reprogramming Techniques / Q000379:methods*; D018274:Electroporation; D019309:Epstein-Barr Virus Nuclear Antigens / Q000235:genetics; D005347:Fibroblasts / Q000166:cytology* / Q000821:virology; D016259:Genes, myc; D005822:Genetic Vectors; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology*; D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors / Q000235:genetics; D008297:Male; D050814:Octamer Transcription Factor-3 / Q000235:genetics; D010012:Osteogenesis; D010957:Plasmids / Q000235:genetics*; D016271:Proto-Oncogene Proteins c-myc; D016601:RNA-Binding Proteins / Q000235:genetics; D055748:SOXB1 Transcription Factors / Q000235:genetics; D029082:Sendai virus; D014161:Transduction, Genetic; D014162:Transfection; D055815:Young Adult
chemical_list: D019309:Epstein-Barr Virus Nuclear Antigens; C000715467:KLF4 protein, human; D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors; C000715187:Lin28A protein, human; C000596782:MYCL protein, human; D050814:Octamer Transcription Factor-3; C494173:POU5F1 protein, human; D016271:Proto-Oncogene Proteins c-myc; D016601:RNA-Binding Proteins; C527782:SOX2 protein, human; D055748:SOXB1 Transcription Factors; C417029:EBV-encoded nuclear antigen 1
keywords: differentiation; induced pluripotent stem cells; reprogramming; transduction; transfection