title: Molecular characterization of the human microbiome from a reproductive perspective.
abstract: The process of reproduction inherently poses unique microbial challenges because it requires the transfer of gametes from one individual to the other, meanwhile preserving the integrity of the gametes and individuals from harmful microbes during the process. Advances in molecular biology techniques have expanded our understanding of the natural organisms living on and in our bodies, including those inhabiting the reproductive tract. Over the past two decades accumulating evidence has shown that the human microbiome is tightly related to health and disease states involving the different body systems, including the reproductive system. Here we introduce the science involved in the study of the human microbiome. We examine common methods currently used to characterize the human microbiome as an inseparable part of the reproductive system. Finally, we consider a few limitations, clinical implications, and the critical need for additional research in the field of human fertility.
authors.affiliation: Department of Obstetrics and Gynecology, Maimonides Medical Center, Brooklyn, New York.
authors.forename: Amir
authors.initials: A
authors.lastname: Mor
authors.affiliation: Department of Gynecology and Obstetrics, Johns Hopkins School of Medicine, Baltimore, Maryland.
authors.forename: Paul H
authors.initials: PH
authors.lastname: Driggers
authors.affiliation: Department of Gynecology and Obstetrics, Johns Hopkins School of Medicine, Baltimore, Maryland. Electronic address: jsegars2@jhmi.edu.
authors.forename: James H
authors.initials: JH
authors.lastname: Segars
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification; D019295:Computational Biology; D004269:DNA, Bacterial / Q000235:genetics* / Q000302:isolation & purification; D064806:Dysbiosis; D005260:Female; D005835:Genitalia / Q000382:microbiology* / Q000503:physiopathology; D054884:Host-Pathogen Interactions; D006801:Humans; D007246:Infertility / Q000382:microbiology / Q000503:physiopathology; D008297:Male; D064307:Microbiota; D025202:Molecular Diagnostic Techniques; D012098:Reproduction; D021521:Ribotyping
chemical_list: D004269:DNA, Bacterial
keywords: DNA sequencing; fertility; microbes; molecular biology; reproductive system