title: Evaluation of the Lipid Interference for Siemens BN ProSpec Cystatin C Assay Using Pediatric Samples.
abstract: Endogenous interferents (lipids, hemoglobin and bilirubin) are a common cause of pre-analytical laboratory errors. We evaluated the effect of lipemia on Siemens cystatin C assay using pediatric samples. Lipemic samples were prepared by adding various concentrations of triglycerides into low and high cystatin C sample pools. Cystatin C concentrations were then measured on Siemens BN ProSpec analyzer and change of the analyte concentrations was determined. Low and high cystatin C sample pools were not affected by additions of lower lipid concentrations (150, 500 and 750 mg/dL), while the negative bias of <10% was seen with additions of higher lipid concentrations (1000 and 1500 mg/dL). Our results suggest that the BN ProSpec assay is a good alternative for cystatin C measurements in our pediatric population with no major interference from lipemia.
authors.affiliation: Department of Pathology & Immunology, Baylor College of Medicine, Houston, TX, USA Texas Children's Hospital, Houston, TX, USA.
authors.forename: Neval
authors.initials: N
authors.lastname: Akbas
authors.affiliation: Texas Children's Hospital, Houston, TX, USA.
authors.forename: Graciela
authors.initials: G
authors.lastname: Gonzalez
authors.affiliation: Department of Pathology & Immunology, Baylor College of Medicine, Houston, TX, USA Texas Children's Hospital, Houston, TX, USA sxdevara@texaschildrens.org.
authors.forename: Sridevi
authors.initials: S
authors.lastname: Devaraj
mesh_terms: D002648:Child; D019963:Clinical Chemistry Tests / Q000295:instrumentation / Q000379:methods*; D055316:Cystatin C / Q000097:blood* / Q000737:chemistry; D006801:Humans; D006949:Hyperlipidemias / Q000097:blood; D008055:Lipids / Q000097:blood / Q000737:chemistry*; D014280:Triglycerides / Q000097:blood
chemical_list: D055316:Cystatin C; D008055:Lipids; D014280:Triglycerides
keywords: Cystatin C; interference; nephelometry; triglycerides