title: Identification of Chromium Resistant Bacteria from Dry Fly Ash Sample of Mejia MTPS Thermal Power Plant, West Bengal, India.
abstract: Eight chromium resistant bacteria were isolated from a dry fly ash sample of DVC-MTPS thermal power plant located in Bankura, West Bengal, India. These isolates displayed different degrees of chromate reduction under aerobic conditions. According to 16S rDNA gene analysis, five of them were Staphylococcus, two were Bacillus and one was Micrococcus. The minimum inhibitory concentration towards chromium and the ability to reduce hexavalent chromium to trivalent chromium was highest in Staphylococcus haemolyticus strain HMR17. All the strains were resistant to multiple heavy metals (As, Cu, Cd, Co, Zn, Mn, Pb and Fe) and reduced toxic hexavalent chromium to relatively non toxic trivalent chromium even in the presence of these multiple heavy metals. All of them showed resistance to different antibiotics. In a soil microcosm study, S. haemolyticus strain HMR17 completely reduced 4 mM hexavalent chromium within 7 days of incubation.
authors.affiliation: Department of Biotechnology, Techno India University, Salt Lake, Sector V, Kolkata, West Bengal, 700 091, India.
authors.forename: Roopali
authors.initials: R
authors.lastname: Roychowdhury
authors.affiliation: Department of Biotechnology, Techno India University, Salt Lake, Sector V, Kolkata, West Bengal, 700 091, India.
authors.forename: Pritam
authors.initials: P
authors.lastname: Mukherjee
authors.affiliation: Department of Biotechnology, Techno India University, Salt Lake, Sector V, Kolkata, West Bengal, 700 091, India. biotech4job@gmail.com.
authors.forename: Madhumita
authors.initials: M
authors.lastname: Roy
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001407:Bacillus / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism*; D001419:Bacteria / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism*; D002857:Chromium / Q000378:metabolism / Q000494:pharmacology*; D060729:Coal Ash / Q000737:chemistry*; D024881:Drug Resistance, Bacterial; D007194:India; D019216:Metals, Heavy / Q000378:metabolism / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D008837:Micrococcus / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism; D011210:Power Plants; D012987:Soil; D013210:Staphylococcus / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D060729:Coal Ash; D019216:Metals, Heavy; D012987:Soil; D002857:Chromium; C074702:chromium hexavalent ion
keywords: Antibiotic resistance; Chromate reducing bacteria; Dry fly ash (DFA); Minimal inhibitory concentration (MIC); Multiple heavy metals; Soil microcosm