title: Increasing the Receptor Tyrosine Kinase EphB2 Prevents Amyloid-β-induced Depletion of Cell Surface Glutamate Receptors by a Mechanism That Requires the PDZ-binding Motif of EphB2 and Neuronal Activity.
abstract: Diverse lines of evidence suggest that amyloid-β (Aβ) peptides causally contribute to the pathogenesis of Alzheimer disease (AD), the most frequent neurodegenerative disorder. However, the mechanisms by which Aβ impairs neuronal functions remain to be fully elucidated. Previous studies showed that soluble Aβ oligomers interfere with synaptic functions by depleting NMDA-type glutamate receptors (NMDARs) from the neuronal surface and that overexpression of the receptor tyrosine kinase EphB2 can counteract this process. Through pharmacological treatments and biochemical analyses of primary neuronal cultures expressing wild-type or mutant forms of EphB2, we demonstrate that this protective effect of EphB2 depends on its PDZ-binding motif and the presence of neuronal activity but not on its kinase activity. We further present evidence that the protective effect of EphB2 may be mediated by the AMPA-type glutamate receptor subunit GluA2, which can become associated with the PDZ-binding motif of EphB2 through PDZ domain-containing proteins and can promote the retention of NMDARs in the membrane. In addition, we show that the Aβ-induced depletion of surface NMDARs does not depend on several factors that have been implicated in the pathogenesis of Aβ-induced neuronal dysfunction, including aberrant neuronal activity, tau, prion protein (PrP(C)), and EphB2 itself. Thus, although EphB2 does not appear to be directly involved in the Aβ-induced depletion of NMDARs, increasing its expression may counteract this pathogenic process through a neuronal activity- and PDZ-dependent regulation of AMPA-type glutamate receptors.
authors.affiliation: From the Gladstone Institute of Neurological Disease, San Francisco, California 94158 and; Department of Neurology, University of California, San Francisco, California 94158.
authors.forename: Takashi
authors.initials: T
authors.lastname: Miyamoto
authors.affiliation: From the Gladstone Institute of Neurological Disease, San Francisco, California 94158 and.
authors.forename: Daniel
authors.initials: D
authors.lastname: Kim
authors.affiliation: From the Gladstone Institute of Neurological Disease, San Francisco, California 94158 and.
authors.forename: Joseph A
authors.initials: JA
authors.lastname: Knox
authors.affiliation: From the Gladstone Institute of Neurological Disease, San Francisco, California 94158 and; Department of Neurology, University of California, San Francisco, California 94158.
authors.forename: Erik
authors.initials: E
authors.lastname: Johnson
authors.affiliation: From the Gladstone Institute of Neurological Disease, San Francisco, California 94158 and; Department of Neurology, University of California, San Francisco, California 94158. Electronic address: Lmucke@gladstone.ucsf.edu.
authors.forename: Lennart
authors.initials: L
authors.lastname: Mucke
mesh_terms: D000544:Alzheimer Disease / Q000235:genetics / Q000378:metabolism*; D020816:Amino Acid Motifs; D016229:Amyloid beta-Peptides / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D005260:Female; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D009474:Neurons / Q000737:chemistry / Q000378:metabolism*; D054731:PDZ Domains; D011485:Protein Binding; D036183:Receptor, EphB2 / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D018091:Receptors, AMPA / Q000235:genetics / Q000378:metabolism; D016194:Receptors, N-Methyl-D-Aspartate / Q000235:genetics / Q000378:metabolism*
chemical_list: D016229:Amyloid beta-Peptides; C121673:Gprin1 protein, mouse; D009419:Nerve Tissue Proteins; D018091:Receptors, AMPA; D016194:Receptors, N-Methyl-D-Aspartate; C586311:Ephb2 protein, mouse; D036183:Receptor, EphB2; C104722:glutamate receptor ionotropic, AMPA 2
keywords: Alzheimer disease; EphB2; N-methyl-D-aspartate receptor (NMDA receptor, NMDAR); PDZ; amyloid-β (Aβ); neurodegeneration; prion; tau; α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPA receptor, AMPAR)