title: Genoprotective and neuroprotective effects of Daphne gnidium leaf methanol extract, tested on male mice.
abstract: Methanol extract of Daphne gnidium leaves was assessed for its antigenotoxic and neuroprotective effects through antioxidant and antibutyrylcholinesterase activities. Antigenotoxic activity was evaluated against methyl methanesulfonate injected intraperitoneally to mice, using the comet assay. The protective effect of D. gnidium reached 99.12%, at the lowest tested dose (44 mg/kg b.w.) in kidney cells, and 92.16% at the dose of 88 mg/kg b.w. in blood cells. The extract was dissolved in water and administrated to mice by intraperitoneal injection. Antioxidant activity was tested against DPPH radicals. It reached a maximum of 74.52% with an IC50 value of 45 µg/ml. Anticholinesterase activity was determined against butyrylcholinesterase, an enzyme linked to Alzheimer disease. The extract exhibited antibutyrylcholinestrase effect with an inhibition percentage of 35.82% at the lowest tested dose (44 mg/kg b.w.).
authors.affiliation: a Unité de Substances Naturelles Bioactives et Biotechnologie, Faculté de Pharmacie de Monastir, Université de Monastir , Monastir , Tunisia and.
authors.forename: Fadwa
authors.initials: F
authors.lastname: Chaabane
authors.affiliation: a Unité de Substances Naturelles Bioactives et Biotechnologie, Faculté de Pharmacie de Monastir, Université de Monastir , Monastir , Tunisia and.
authors.forename: Imen
authors.initials: I
authors.lastname: Mokdad-Bzeouich
authors.affiliation: a Unité de Substances Naturelles Bioactives et Biotechnologie, Faculté de Pharmacie de Monastir, Université de Monastir , Monastir , Tunisia and.
authors.forename: Aicha
authors.initials: A
authors.lastname: Sassi
authors.affiliation: a Unité de Substances Naturelles Bioactives et Biotechnologie, Faculté de Pharmacie de Monastir, Université de Monastir , Monastir , Tunisia and.
authors.forename: Nadia
authors.initials: N
authors.lastname: Mustapha
authors.affiliation: a Unité de Substances Naturelles Bioactives et Biotechnologie, Faculté de Pharmacie de Monastir, Université de Monastir , Monastir , Tunisia and.
authors.forename: Raja
authors.initials: R
authors.lastname: Majouli
authors.affiliation: a Unité de Substances Naturelles Bioactives et Biotechnologie, Faculté de Pharmacie de Monastir, Université de Monastir , Monastir , Tunisia and.
authors.forename: Kamel
authors.initials: K
authors.lastname: Ghedira
authors.affiliation: a Unité de Substances Naturelles Bioactives et Biotechnologie, Faculté de Pharmacie de Monastir, Université de Monastir , Monastir , Tunisia and.|b Laboratoire de Biologie Moléculaire et Cellulaire , Faculté de Médecine Dentaire de Monastir, Université de Monastir , Monastir , Tunisia.
authors.forename: Leila
authors.initials: L
authors.lastname: Chekir-Ghedira
mesh_terms: D000818:Animals; D016587:Antimutagenic Agents / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D001713:Biphenyl Compounds / Q000737:chemistry; D002091:Butyrylcholinesterase / Q000097:blood; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects* / Q000235:genetics; D032364:Daphne / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D007274:Injections, Intraperitoneal; D007668:Kidney / Q000187:drug effects / Q000473:pathology; D007928:Lethal Dose 50; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D000432:Methanol / Q000737:chemistry; D008741:Methyl Methanesulfonate / Q000633:toxicity; D051379:Mice; D018696:Neuroprotective Agents / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D018515:Plant Leaves / Q000737:chemistry*; D023382:Toxicity Tests, Acute
chemical_list: D016587:Antimutagenic Agents; D001713:Biphenyl Compounds; D018696:Neuroprotective Agents; D010851:Picrates; D010936:Plant Extracts; D008741:Methyl Methanesulfonate; C004931:1,1-diphenyl-2-picrylhydrazyl; D002091:Butyrylcholinesterase; D000432:Methanol
keywords: Anticholinesterasic activity; Daphne gnidium; antigenotoxic; antioxidant; comet assay