title: Fucoidans Disrupt Adherence of Helicobacter pylori to AGS Cells In Vitro.
abstract: Fucoidans are complex sulphated polysaccharides derived from abundant and edible marine algae. Helicobacter pylori is a stomach pathogen that persists in the hostile milieu of the human stomach unless treated with antibiotics. This study aims to provide preliminary data to determine, in vitro, if fucoidans can inhibit the growth of H. pylori and its ability to adhere to gastric epithelial cells (AGS). We analysed the activity of three different fucoidan preparations (Fucus A, Fucus B, and Undaria extracts). Bacterial growth was not arrested or inhibited by the fucoidan preparations supplemented into culture media. All fucoidans, when supplemented into tissue culture media at 1000 µg mL(-1), were toxic to AGS cells and reduced the viable cell count significantly. Fucoidan preparations at 100 µg mL(-1) were shown to significantly reduce the number of adherent H. pylori. These in vitro findings provide the basis for further studies on the clinical use of sulphated polysaccharides as complementary therapeutic agents.
authors.affiliation: The Marshall Centre for Infectious Diseases Research and Training, School of Pathology and Laboratory Medicine (M502), The University of Western Australia, 35 Stirling Highway, Nedlands, WA 6009, Australia.
authors.forename: Eng-Guan
authors.initials: EG
authors.lastname: Chua
authors.affiliation: The Marshall Centre for Infectious Diseases Research and Training, School of Pathology and Laboratory Medicine (M502), The University of Western Australia, 35 Stirling Highway, Nedlands, WA 6009, Australia.
authors.forename: Phebe
authors.initials: P
authors.lastname: Verbrugghe
authors.affiliation: The Marshall Centre for Infectious Diseases Research and Training, School of Pathology and Laboratory Medicine (M502), The University of Western Australia, 35 Stirling Highway, Nedlands, WA 6009, Australia.
authors.forename: Timothy T
authors.initials: TT
authors.lastname: Perkins
authors.affiliation: The Marshall Centre for Infectious Diseases Research and Training, School of Pathology and Laboratory Medicine (M502), The University of Western Australia, 35 Stirling Highway, Nedlands, WA 6009, Australia.
authors.forename: Chin-Yen
authors.initials: CY
authors.lastname: Tay