title: Proteomic analyses provide new insights into the responses of Pinus massoniana seedlings to phosphorus deficiency.
abstract: Phosphorus is an essential macronutrient for plant growth and development. Plants can respond defensively to phosphorus deficiency by modifying their morphology and metabolic pathways via the differential expression of low phosphate responsive genes. To better understand the mechanisms by which the Masson pine (Pinus massoniana) adapts to phosphorus deficiency, we conducted comparative proteomic analysis using an elite line exhibiting high tolerance to phosphorus deficiency. The selected seedlings were treated with 0.5 mM KH2PO4 (control), 0.01 mM KH2PO4 (P1), or 0.06 mM KH2PO4 (P2) for 48 days. Total protein samples were separated via 2DE. A total of 98 differentially expressed proteins, which displayed at least 1.7-fold change expression compared to the control levels (p ≤ 0.05), were identified by MALDI-TOF/TOF MS. These phosphate starvation responsive proteins were implicated in photosynthesis, defense, cellular organization, biosynthesis, energy metabolism, secondary metabolism, signal transduction etc. Therefore, these proteins might play important roles in facilitating internal phosphorus homeostasis. Additionally, the obtained data may be useful for the further characterization of gene function and may provide a foundation for a more comprehensive understanding of the adaptations of the Masson pine to phosphorus-deficient conditions.
authors.affiliation: The Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), Institute of Agro-Bioengineering and College of Life Sciences, Guizhou University, Guiyang, Guizhou, P. R. China.|Research Center for Forest Resources and Environment of Guizhou Province, Guizhou University, Guiyang, Guizhou, P. R. China.
authors.forename: Fuhua
authors.initials: F
authors.lastname: Fan
authors.affiliation: Research Center for Forest Resources and Environment of Guizhou Province, Guizhou University, Guiyang, Guizhou, P. R. China.
authors.forename: Guijie
authors.initials: G
authors.lastname: Ding
authors.affiliation: The Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), Institute of Agro-Bioengineering and College of Life Sciences, Guizhou University, Guiyang, Guizhou, P. R. China.|Collaborative Innovation Center for Mountain Ecology & Agro-Bioengineering (CICEAB), Institute of Agro-Bioengineering, College of Life Science, Guizhou University, Guiyang, Guizhou, P. R. China.
authors.forename: Xiaopeng
authors.initials: X
authors.lastname: Wen
mesh_terms: D000222:Adaptation, Physiological; D015180:Electrophoresis, Gel, Two-Dimensional; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D063990:Gene Ontology; D053858:Metabolic Networks and Pathways; D058977:Molecular Sequence Annotation; D010758:Phosphorus / Q000172:deficiency*; D028223:Pinus / Q000235:genetics / Q000378:metabolism*; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D020543:Proteome / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D036226:Seedlings / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013312:Stress, Physiological
chemical_list: D010940:Plant Proteins; D020543:Proteome; D010758:Phosphorus
keywords: Masson pine; Phosphorus deficiency; Plant proteomics; Two-dimensional gel electrophoresis