title: Elevated CO2-induced production of nitric oxide (NO) by NO synthase differentially affects nitrate reductase activity in Arabidopsis plants under different nitrate supplies.
abstract: CO2 elevation often alters the plant's nitrate reductase (NR) activity, the first enzyme acting in the nitrate assimilation pathway. However, the mechanism underlying this process remains unknown. The association between elevated CO2-induced alterations of NR activity and nitric oxide (NO) was examined in Col-0 Arabidopsis fed with 0.2-10 mM nitrate, using NO donors, NO scavenger, and NO synthase (NOS) inhibitor. The noa1 mutant, in which most NOS activity was lost, and the NR activity-null mutant nia1 nia2 were also used to examine the above association. In response to CO2 elevation, NR activity increased in low-nitrate Col-0 plants but was inhibited in high-nitrate Col-0 plants. NO scavenger and NOS inhibitor could eliminate these two responses, whereas the application of NO donors mimicked these distinct responses in ambient CO2-grown Col-0 plants. Furthermore, in both low- and high-nitrate conditions, elevated CO2 increased NOS activity and NO levels in Col-0 and nia1 nia2 plants but had little effect on NO level and NR activity in noa1 plants. Considering all of these findings, this study concluded that, in response to CO2 elevation, either the NR activity induction in low-nitrate plants or the NR activity inhibition in high-nitrate plants is regulated by NOS-generated NO.
authors.affiliation: College of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310018, PR China dushaoting@zjgsu.edu.cn.
authors.forename: Shaoting
authors.initials: S
authors.lastname: Du
authors.affiliation: College of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310018, PR China.
authors.forename: Ranran
authors.initials: R
authors.lastname: Zhang
authors.affiliation: College of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310018, PR China.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhang
authors.affiliation: College of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310018, PR China.
authors.forename: Huijun
authors.initials: H
authors.lastname: Liu
authors.affiliation: College of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310018, PR China.
authors.forename: Minggang
authors.initials: M
authors.lastname: Yan
authors.affiliation: College of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310018, PR China.
authors.forename: Ni
authors.initials: N
authors.lastname: Chen
authors.affiliation: College of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310018, PR China.
authors.forename: Huaqiang
authors.initials: H
authors.lastname: Xie
authors.affiliation: College of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310018, PR China.
authors.forename: Shouwei
authors.initials: S
authors.lastname: Ke
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D001565:Benzoates / Q000494:pharmacology; D002245:Carbon Dioxide / Q000494:pharmacology*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D007093:Imidazoles / Q000494:pharmacology; D008954:Models, Biological; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D050901:Nitrate Reductase / Q000378:metabolism*; D009566:Nitrates / Q000494:pharmacology*; D009569:Nitric Oxide / Q000096:biosynthesis*; D019001:Nitric Oxide Synthase / Q000378:metabolism*; D018515:Plant Leaves / Q000187:drug effects / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects / Q000378:metabolism; D013997:Time Factors; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D001565:Benzoates; D007093:Imidazoles; D009566:Nitrates; D002245:Carbon Dioxide; C079393:1,3-dihydroxy-4,4,5,5-tetramethyl-2-(4-carboxyphenyl)tetrahydroimidazole; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase; D050901:Nitrate Reductase; D019331:NG-Nitroarginine Methyl Ester
keywords: CO2; NO synthase; S-nitrosylation.; elevated CO2; nitrate; nitrate reductase; nitric oxide