title: Optimal concentration and time window for proliferation and differentiation of neural stem cells from embryonic cerebral cortex: 5% oxygen preconditioning for 72 hours.
abstract: Hypoxia promotes proliferation and differentiation of neural stem cells from embryonic day 12 rat brain tissue, but the concentration and time of hypoxic preconditioning are controversial. To address this, we cultured neural stem cells isolated from embryonic day 14 rat cerebral cortex in 5% and 10% oxygen in vitro. MTT assay, neurosphere number, and immunofluorescent staining found that 5% or 10% oxygen preconditioning for 72 hours improved neural stem cell viability and proliferation. With prolonged hypoxic duration (120 hours), the proportion of apoptotic cells increased. Thus, 5% oxygen preconditioning for 72 hours promotes neural stem cell proliferation and neuronal differentiation. Our findings indicate that the optimal concentration and duration of hypoxic preconditioning for promoting proliferation and differentiation of neural stem cells from the cerebral cortex are 5% oxygen for 72 hours.
authors.affiliation: Department of Histology and Embryology, Academy of Basic Medicine, Jining Medical University, Jining, Shandong Province, China ; Department of Histology and Embryology, Academy of Basic Medicine, Weifang Medical University, Weifang, Shandong Province, China.
authors.forename: Li-Li
authors.identifier: 0000-0002-3689-7847
authors.initials: LL
authors.lastname: Yuan
authors.affiliation: Department of Histology and Embryology, Academy of Basic Medicine, Weifang Medical University, Weifang, Shandong Province, China.
authors.forename: Ying-Jun
authors.identifier: 0000-0002-9578-0497
authors.initials: YJ
authors.lastname: Guan
authors.affiliation: Department of Otorhinolaryngology, Affiliated Hospital of Jining Medical University, Jining, Shandong Province, China.
authors.forename: Deng-Dian
authors.initials: DD
authors.lastname: Ma
authors.affiliation: Department of Histology and Embryology, Academy of Basic Medicine, Weifang Medical University, Weifang, Shandong Province, China.
authors.forename: Hong-Mei
authors.initials: HM
authors.lastname: Du
keywords: apoptosis; brain injury; caspase-3; cerebral cortex; differentiation; glial fibrillary acidic protein; low oxygen; microtubule-associated protein 2; nerve regeneration; neural regeneration; neural stem cells