title: Triclosan and bisphenol a affect decidualization of human endometrial stromal cells.
abstract: In recent years, impaired fertility and endometrium related diseases are increased. Many evidences suggest that environmental pollution might be considered a risk factor for endometrial physiopathology. Among environmental pollutants, endocrine disrupting chemicals (EDCs) act on endocrine system, causing hormonal imbalance which, in turn, leads to female and male reproductive dysfunctions. In this work, we studied the effects of triclosan (TCL) and bisphenol A (BPA), two widespread EDCs, on human endometrial stromal cells (ESCs), derived from endometrial biopsies from woman not affected by endometriosis. Cell proliferation, cell cycle, migration and decidualization mechanisms were investigated. Treatments have been performed with both the EDCs separately or in presence and in absence of progesterone used as decidualization stimulus. Both TCL and BPA did not affect cell proliferation, but they arrested ESCs at G2/M phase of cell cycle enhancing cell migration. TCL and BPA also increased gene expression and protein levels of some decidualization markers, such as insulin growth factor binding protein 1 (IGFBP1) and prolactin (PRL), amplifying the effect of progesterone alone. All together, our data strongly suggest that TCL and BPA might alter human endometrium physiology so affecting fertility and pregnancy outcome.
authors.affiliation: National Laboratory on Endocrine Disruptors of Interuniversity Consortium INBB, Italy; Institute of Genetics and Biophysics (IGB) "ABT", National Research Council (CNR), Via P. Castellino, 111, 80131, Naples, Italy.
authors.forename: Maurizio
authors.initials: M
authors.lastname: Forte
authors.affiliation: National Laboratory on Endocrine Disruptors of Interuniversity Consortium INBB, Italy; Institute of Genetics and Biophysics (IGB) "ABT", National Research Council (CNR), Via P. Castellino, 111, 80131, Naples, Italy.
authors.forename: Luigi
authors.initials: L
authors.lastname: Mita
authors.affiliation: Department of Gynaecology, Obstetric and Reproductive Science, Second University of Studies of Naples, Naples, Italy.
authors.forename: Luigi
authors.initials: L
authors.lastname: Cobellis
authors.affiliation: Institute of Genetics and Biophysics (IGB) "ABT", National Research Council (CNR), Via P. Castellino, 111, 80131, Naples, Italy.
authors.forename: Verdiana
authors.initials: V
authors.lastname: Merafina
authors.affiliation: Institute of Genetics and Biophysics (IGB) "ABT", National Research Council (CNR), Via P. Castellino, 111, 80131, Naples, Italy.
authors.forename: Raffaella
authors.initials: R
authors.lastname: Specchio
authors.affiliation: Institute of Genetics and Biophysics (IGB) "ABT", National Research Council (CNR), Via P. Castellino, 111, 80131, Naples, Italy.
authors.forename: Sergio
authors.initials: S
authors.lastname: Rossi
authors.affiliation: National Laboratory on Endocrine Disruptors of Interuniversity Consortium INBB, Italy; Institute of Genetics and Biophysics (IGB) "ABT", National Research Council (CNR), Via P. Castellino, 111, 80131, Naples, Italy. Electronic address: mita@igb.cnr.it.
authors.forename: Damiano Gustavo
authors.initials: DG
authors.lastname: Mita
authors.affiliation: Department of Gynaecology, Obstetric and Reproductive Science, Second University of Studies of Naples, Naples, Italy.
authors.forename: Lavinia
authors.initials: L
authors.lastname: Mosca
authors.affiliation: Department of Gynaecology, Obstetric and Reproductive Science, Second University of Studies of Naples, Naples, Italy.
authors.forename: Maria Antonietta
authors.initials: MA
authors.lastname: Castaldi
authors.affiliation: National Laboratory on Endocrine Disruptors of Interuniversity Consortium INBB, Italy; Department of Biology, University "Federico II" of Naples, Naples, Italy.
authors.forename: Maria
authors.initials: M
authors.lastname: De Falco
authors.affiliation: National Laboratory on Endocrine Disruptors of Interuniversity Consortium INBB, Italy; Department of Biology, University "Federico II" of Naples, Naples, Italy.
authors.forename: Vincenza
authors.initials: V
authors.lastname: Laforgia
authors.affiliation: National Laboratory on Endocrine Disruptors of Interuniversity Consortium INBB, Italy; Institute of Biosciences and Bioresources (IBBR), National Research Council (CNR), Naples, Italy.
authors.forename: Stefania
authors.initials: S
authors.lastname: Crispi
mesh_terms: D000328:Adult; D002453:Cell Cycle / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003656:Decidua / Q000166:cytology* / Q000378:metabolism; D052244:Endocrine Disruptors / Q000009:adverse effects*; D004717:Endometrium / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D005260:Female; D006801:Humans; D018970:Insulin-Like Growth Factor Binding Protein 1 / Q000235:genetics / Q000378:metabolism; D008875:Middle Aged; D011374:Progesterone / Q000494:pharmacology; D011388:Prolactin / Q000235:genetics / Q000378:metabolism; D017154:Stromal Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D014260:Triclosan / Q000009:adverse effects*; D015854:Up-Regulation
chemical_list: D052244:Endocrine Disruptors; C032231:IGFBP1 protein, human; D018970:Insulin-Like Growth Factor Binding Protein 1; D011374:Progesterone; D014260:Triclosan; D011388:Prolactin
keywords: Bisphenol A; Decidualization; Endocrine disrupting chemicals; Endometrium; Triclosan