title: Streptomyces spongiicolasp. nov., an actinomycete derived from marine sponge.
abstract: A novel actinomycete (strain HNM0071T) was isolated from an unidentified marine sponge collected from the coast of Sanya City, PR China and its taxonomic position was investigated. The major menaquinones were MK-9 (H6) (65.6 %), MK-9 (H4) (23.8 %) and MK-9 (H8) (10.6 %). The predominant fatty acids were anteiso-C15 : 0(25.5 %), iso-C16 : 0(19.5 %) and iso-C15 : 0(15.4 %). The predominant phospholipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. In addition, four unidentified phospholipids were found. The G+C content of the genomic DNA was 69.8 mol%. Based on 16S rRNA gene sequence analysis, strain HNM0071Twas most closely related toStreptomyces wuyuanensisFX61T(97.3 %). The 16S rRNA gene sequence similarity to all other species of the genusStreptomyceswas less than 97.0 %. DNA-DNA hybridization between strain HNM0071Tand its closest phylogenetic relative,Streptomyces wuyuanensisFX61T, showed 25.0 % relatedness. Based on phenotypic and genotypic characteristics, strain HNM0071Trepresents a novel species of the genusStreptomyceswhose name is proposed asStreptomyes spongiicolasp. nov. The type strain is HNM0071T( = CCTCCAA2015018T= KCTC 39604T).
authors.affiliation: College of Agriculture, Hainan University, Haikou 570228, PRChina.|Key Laboratory of Marine Drugs, Ministry of Education of China, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, PRChina.
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Huang
authors.affiliation: College of Agriculture, Hainan University, Haikou 570228, PRChina.
authors.forename: Shuangqing
authors.initials: S
authors.lastname: Zhou
authors.affiliation: College of Agriculture, Hainan University, Haikou 570228, PRChina.
authors.forename: Dongyi
authors.initials: D
authors.lastname: Huang
authors.affiliation: College of Agriculture, Hainan University, Haikou 570228, PRChina.
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.affiliation: Key Laboratory of Marine Drugs, Ministry of Education of China, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, PRChina.
authors.forename: Weiming
authors.initials: W
authors.lastname: Zhu