title: Bacteremia due to Pasteurella dagmatis acquired from a dog bite, with a review of systemic infections and challenges in laboratory identification.
abstract: A case of bacteremia in a 74-year-old man, which was caused by Pasteurella dagmatis and complicated by thrombocytopenia, is presented. Microorganism identification was performed by the provincial reference laboratory using traditional biochemical profiling, completmented with both the sequencing of the 16S ribosomal RNA gene and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry; antibiotic-susceptibility testing was also performed. After treatment with the appropriate antibiotics, the patient fully recovered. Systemic infections attributed to this organism are rarely reported in the literature. Other reported cases of bacteremia due to P dagmatis are reviewed and compared with the present case. The challenges of relying on standard automatic identification are discussed, with alternative methodologies provided.
authors.affiliation: Department of Laboratory Medicine, Public Health Ontario;
authors.forename: Jianhui
authors.initials: J
authors.lastname: Xiong
authors.affiliation: Division of Infectious Diseases, Public Health Ontario; ; Internal Medicine of St Joseph's Health Centre, Public Health Ontario; ; Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario.
authors.forename: Sigmund
authors.initials: S
authors.lastname: Krajden
authors.affiliation: Public Health Ontario; ; Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario.
authors.forename: Julianne V
authors.initials: JV
authors.lastname: Kus
authors.affiliation: Public Health Ontario;
authors.forename: Prasad
authors.initials: P
authors.lastname: Rawte
authors.affiliation: Internal Medicine of St Joseph's Health Centre, Public Health Ontario;
authors.forename: John
authors.initials: J
authors.lastname: Blondal
authors.affiliation: Division of Infectious Diseases, Public Health Ontario; ; Internal Medicine of St Joseph's Health Centre, Public Health Ontario;
authors.forename: Mark
authors.initials: M
authors.lastname: Downing
authors.affiliation: Internal Medicine of St Joseph's Health Centre, Public Health Ontario;
authors.forename: Urszula
authors.initials: U
authors.lastname: Zurawska
authors.affiliation: Department of Laboratory Medicine, Public Health Ontario; ; Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario.
authors.forename: William
authors.initials: W
authors.lastname: Chapman
keywords: 16S rRNA gene sequencing; Bacteremia; Dog bite; Identification; MALDI-ToF MS; Pasteurella dagmatis; VITEK 2