title: Cell suspension concentration monitoring by using a miniaturized serial high frequency SAWR sensor.
abstract: In this paper, a miniaturized cell suspension concentration monitoring method was investigated. The sensing unit was a carbon screen-printed electrode (CSPE) in serial with a 433MHz vacuum-packaged surface acoustic wave resonator (SAWR). SAWR provided a stable and high operating frequency, which helps to keep the stability and sensitivity of the monitoring system. Living cells suspensions in different concentrations were prepared and dropcast on CSPE. Frequency responses of the sensor were recorded. Cell quantity variation within the same culture media volume changed the dielectric properties of CSPE and finally affected the SAWR frequency. SAWR frequency declined with the decrease of cell concentration. The proposed sensor provided high sensitivity and remarkable stability for the cell suspensions.
authors.affiliation: a School of Information Engineering; Key Laboratory of Forestry Intelligent Monitoring and Information Technology of Zhejiang Province; Zhejiang A & F University ; Linan , China.
authors.forename: Jian
authors.initials: J
authors.lastname: Li
authors.affiliation: a School of Information Engineering; Key Laboratory of Forestry Intelligent Monitoring and Information Technology of Zhejiang Province; Zhejiang A & F University ; Linan , China.
authors.forename: Hailin
authors.initials: H
authors.lastname: Feng
authors.affiliation: a School of Information Engineering; Key Laboratory of Forestry Intelligent Monitoring and Information Technology of Zhejiang Province; Zhejiang A & F University ; Linan , China.
authors.forename: Yiming
authors.initials: Y
authors.lastname: Fang
mesh_terms: D000162:Acoustics; D057005:Bioengineering / Q000295:instrumentation; D015374:Biosensing Techniques / Q000295:instrumentation*; D002244:Carbon; D002448:Cell Adhesion; D049109:Cell Proliferation; D003199:Computer Systems; D004867:Equipment Design; D006801:Humans; D020014:K562 Cells; D008904:Miniaturization; D014618:Vacuum
chemical_list: D002244:Carbon
keywords: carbon screen-printed electrode; cell suspension concentration; frequency; serial; surface acoustic wave