title: Ginsenoside Rg1 prevents cerebral and cerebellar injury induced by obstructive jaundice in rats via inducing expression of TIPE-2.
abstract: The aim of the study was to analyze the effect of Ginsenoside Rg1 (Rg1) on cerebral and cerebellar injury in experimental obstructive jaundice (OJ). OJ was done by ligature and section of extrahepatic biliary duct. Rg1 was injected intraperitoneally (10 mg kg(-1)d(-1) or 20 mg kg(-1) d(-1)). Comparison of serum total bile salts (TBA), total bilirubin (TBil), direct bilirubin (DBil), TNF-α, IL-6 and IL-1β among groups. Malondialdehyde (MDA), glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) were determined, also apoptosis and mRNA and protein levels of TIPE2 (TNF-α-inducible protein 8-like 2) were tested in cerebrum and cerebellum. Our results showed that Rg1 reduced MDA and apoptosis in cerebrum and cerebellum induced by OJ, also GSH and antioxidant enzyme activity were raised obviously in rats treated with Rg1. Moreover, decreased mRNA and protein levels of TIPE2 in OJ rats and Rg1 could improve the decreased mRNA and protein levels of TIPE2 in OJ rats. In conclusion, Rg1 decreased oxidative stress and apoptosis, also recovered the antioxidant status and mRNA and protein levels of TIPE2 in the cerebrum and cerebellum of OJ rats.
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Dalian Medical University, Dalian 116011, PR China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Zhou
authors.affiliation: Department of General Surgery, The Second Affiliated Hospital of Dalian Medical University, Dalian 116023, PR China.
authors.forename: Guo
authors.initials: G
authors.lastname: Zu
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Dalian Medical University, Dalian 116011, PR China.
authors.forename: Lu
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Department of Neurosurgery, The Second Affiliated Hospital of Dalian Medical University, Dalian 116023, PR China.
authors.forename: Ningwei
authors.initials: N
authors.lastname: Che
authors.affiliation: Department of Surgical Operation, Dalian Medical University, Dalian 116044, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Guo
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Dalian Medical University, Dalian 116011, PR China. Electronic address: zhanhualiangdl@163.com.
authors.forename: Zhanhua
authors.initials: Z
authors.lastname: Liang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002531:Cerebellum / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D054022:Cerebrum / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D036145:Ginsenosides / Q000627:therapeutic use*; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D041781:Jaundice, Obstructive / Q000150:complications / Q000188:drug therapy*; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D012333:RNA, Messenger / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D015854:Up-Regulation
chemical_list: D036145:Ginsenosides; D047908:Intracellular Signaling Peptides and Proteins; D012333:RNA, Messenger; C553862:Tnfaip8l2 protein, rat; C035054:ginsenoside Rg1
keywords: Apoptosis; Cerebellum; Cerebrum; Ginsenoside Rg1; Obstructive jaundice; Oxidative stress; TIPE-2