title: Two-phase photoperiodic cultivation of algal-bacterial consortia for high biomass production and efficient nutrient removal from municipal wastewater.
abstract: This study investigated the photoperiodic effects on the biomass production and nutrient removal in the algal-bacterial wastewater treatment, under the following three conditions: (1) a natural 12h:12h LD cycle, (2) a dark-elongated 12h:60h LD cycle, and (3) a two-phase photoperiodic 12h:60h LD, followed by 12h:12h LD cycles. The two-phase photoperiodic operation showed the highest dry cell weight and lipid productivity (282.6mgL(-1)day(-1), 71.4mgL(-1)day(-1)) and most efficient nutrient removals (92.3% COD, 95.8% TN, 98.1% TP). The genetic markers and sequencing analyses indicated rapid increments of bacteria, subsequent growths of Scenedesmus, and stabilized population balances between algae and bacteria. In addition, the two-phase photoperiod provided a higher potential for the algal-bacterial consortia to utilize various organic carbon substrates.
authors.affiliation: Integrated Biorefinery Research Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, Republic of Korea.
authors.forename: Chang Soo
authors.initials: CS
authors.lastname: Lee
authors.affiliation: Integrated Biorefinery Research Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, Republic of Korea.
authors.forename: Hyung-Seok
authors.initials: HS
authors.lastname: Oh
authors.affiliation: Integrated Biorefinery Research Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, Republic of Korea.
authors.forename: Hee-Mock
authors.initials: HM
authors.lastname: Oh
authors.affiliation: Integrated Biorefinery Research Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, Republic of Korea.
authors.forename: Hee-Sik
authors.initials: HS
authors.lastname: Kim
authors.affiliation: Integrated Biorefinery Research Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, Republic of Korea. Electronic address: cyahn@kribb.re.kr.
authors.forename: Chi-Yong
authors.initials: CY
authors.lastname: Ahn
mesh_terms: D001419:Bacteria / Q000254:growth & development* / Q000378:metabolism; D001673:Biodegradation, Environmental; D018533:Biomass; D002244:Carbon / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods*; D005227:Fatty Acids / Q000378:metabolism; D018628:Gene Dosage; D009584:Nitrogen / Q000302:isolation & purification; D010758:Phosphorus / Q000302:isolation & purification; D017440:Photoperiod; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D044444:Scenedesmus / Q000254:growth & development* / Q000378:metabolism; D062065:Wastewater / Q000382:microbiology*; D014874:Water Pollutants, Chemical / Q000302:isolation & purification*
chemical_list: D002244:Carbon; D005227:Fatty Acids; D009584:Nitrogen; D010758:Phosphorus; D012336:RNA, Ribosomal, 16S; D062065:Wastewater; D014874:Water Pollutants, Chemical
keywords: Algae; Bacteria; Biodiesel; Photoperiod; Wastewater