title: Enhancing cellulose accessibility of corn stover by deep eutectic solvent pretreatment for butanol fermentation.
abstract: In this study, an effective corn stover (CS) pretreatment method was developed for biobutanol fermentation. Deep eutectic solvents (DESs), consisted of quaternary ammonium salts and hydrogen donors, display similar properties to room temperature ionic liquid. Seven DESs with different hydrogen donors were facilely synthesized. Choline chloride:formic acid (ChCl:formic acid), an acidic DES, displayed excellent performance in the pretreatment of corn stover by removal of hemicellulose and lignin as confirmed by SEM, FTIR and XRD analysis. After optimization, glucose released from pretreated CS reached 17.0 g L(-1) and yield of 99%. The CS hydrolysate was successfully utilized in butanol fermentation by Clostridium saccharobutylicum DSM 13864, achieving butanol titer of 5.63 g L(-1) with a yield of 0.17 g g(-1) total sugar and productivity of 0.12 g L(-1)h(-1). This study demonstrates DES could be used as a promising and biocompatible pretreatment method for the conversion of lignocellulosic biomass into biofuel.
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, Jiangsu, China.
authors.forename: Guo-Chao
authors.initials: GC
authors.lastname: Xu
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, Jiangsu, China.
authors.forename: Ji-Cai
authors.initials: JC
authors.lastname: Ding
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, Jiangsu, China.
authors.forename: Rui-Zhi
authors.initials: RZ
authors.lastname: Han
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, Jiangsu, China.
authors.forename: Jin-Jun
authors.initials: JJ
authors.lastname: Dong
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, Jiangsu, China. Electronic address: yni@jiangnan.edu.cn.
authors.forename: Ye
authors.initials: Y
authors.lastname: Ni
mesh_terms: D056804:Biofuels; D018533:Biomass; D000440:Butanols / Q000737:chemistry / Q000378:metabolism*; D002482:Cellulose / Q000737:chemistry* / Q000378:metabolism; D002794:Choline / Q000737:chemistry; D003013:Clostridium / Q000378:metabolism*; D003246:Conservation of Energy Resources / Q000379:methods; D005285:Fermentation; D005561:Formates / Q000737:chemistry; D005947:Glucose / Q000737:chemistry / Q000378:metabolism; D006868:Hydrolysis; D008031:Lignin / Q000737:chemistry / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry; D012997:Solvents / Q000737:chemistry; D003313:Zea mays / Q000737:chemistry* / Q000378:metabolism
chemical_list: D056804:Biofuels; D000440:Butanols; D005561:Formates; D011134:Polysaccharides; D012997:Solvents; C030544:formic acid; C007916:hemicellulose; D002482:Cellulose; D008031:Lignin; D005947:Glucose; D002794:Choline
keywords: Butanol fermentation; Corn stover; Deep eutectic solvent; Ionic liquid; Pretreatment