title: Probing astrocyte metabolism in vivo: proton magnetic resonance spectroscopy in the injured and aging brain.
abstract: Following a brain injury, the mobilization of reactive astrocytes is part of a complex neuroinflammatory response that may have both harmful and beneficial effects. There is also evidence that astrocytes progressively accumulate in the normal aging brain, increasing in both number and size. These astrocyte changes in normal brain aging may, in the event of an injury, contribute to the exacerbated injury response and poorer outcomes observed in older traumatic brain injury (TBI) survivors. Here we present our view that proton magnetic resonance spectroscopy ((1)H-MRS), a neuroimaging approach that probes brain metabolism within a defined region of interest, is a promising technique that may provide insight into astrocyte metabolic changes in the injured and aging brain in vivo. Although (1)H-MRS does not specifically differentiate between cell types, it quantifies certain metabolites that are highly enriched in astrocytes (e.g., Myo-inositol, mlns), or that are involved in metabolic shuttling between astrocytes and neurons (e.g., glutamate and glutamine). Here we focus on metabolites detectable by (1)H-MRS that may serve as markers of astrocyte metabolic status. We review the physiological roles of these metabolites, discuss recent (1)H-MRS findings in the injured and aging brain, and describe how an astrocyte metabolite profile approach might be useful in clinical medicine and clinical trials.
authors.affiliation: Hoglund Brain Imaging Center, University of Kansas Medical Center Kansas City, KS, USA ; Department of Anatomy and Cell Biology, University of Kansas Medical Center Kansas City, KS, USA.
authors.forename: Janna L
authors.initials: JL
authors.lastname: Harris
authors.affiliation: Hoglund Brain Imaging Center, University of Kansas Medical Center Kansas City, KS, USA ; Department of Neurology, University of Kansas Medical Center Kansas City, KS, USA ; Department of Molecular and Integrative Physiology, University of Kansas Medical Center Kansas City, KS, USA.
authors.forename: In-Young
authors.initials: IY
authors.lastname: Choi
authors.affiliation: Hoglund Brain Imaging Center, University of Kansas Medical Center Kansas City, KS, USA ; Department of Neurology, University of Kansas Medical Center Kansas City, KS, USA ; Department of Molecular and Integrative Physiology, University of Kansas Medical Center Kansas City, KS, USA.
authors.forename: William M
authors.initials: WM
authors.lastname: Brooks
keywords: aging; astrocytes; glutamic acid; glutathione; lactic acid; myo-inositol; proton magnetic resonance spectroscopy; traumatic brain injury (TBI)