title: Nanochelating based nanocomplex, GFc7, improves quality and quantity of human mesenchymal stem cells during in vitro expansion.
abstract: BACKGROUND
  Human mesenchymal stem cells (hMSCs) have been approved for therapeutic applications. Despite the advances in this field, in vitro approaches are still required to improve the essential indices that would pave the way to a bright horizon for an efficient transplantation in the future. Nanotechnology could help to improve these approaches. Studies signified the important role of iron in stem cell metabolism and efficiency of copper chelation application for stem cell expansion
  
  
  METHODS
  For the first time, based on novel Nanochelating technology, we design an iron containing copper chelator nano complex, GFc7 and examined on hMSCs during in vitro expansion. In this study, the hMSCs were isolated, characterized and expanded in vitro in two media (with or without GFc7). Then proliferation, cell viability, cell cycle analysis, surface markers, HLADR, pluripotency genes expression, homing and antioxidative defense at genes and protein expression were investigated. Also we analyzed the spontaneous differentiation and examined osteogenic and lipogenic differentiation.
  
  
  RESULTS
  GFc7 affected the expression of key genes, improving both the stemness and fitness of the cells in a precise and balanced manner. We observed significant increases in cell proliferation, enhanced expression of pluripotency genes and homing markers, improved antioxidative defense, repression of genes involved in spontaneous differentiation and exposing the hMSCs to differentiation medium indicated that pretreatment with GFc7 increased the quality and rate of differentiation.
  
  
  CONCLUSIONS
  Thus, GFc7 appears to be a potential new supplement for cell culture medium for increasing the efficiency of transplantation.
authors.affiliation: Stem Cell Technology Research Center, Tehran, Iran. mhafizi@nanochelatingtechnology.com.|Department of Research and Development, Sodour Ahrar Shargh Company, Tehran, Iran. mhafizi@nanochelatingtechnology.com.
authors.forename: Maryam
authors.initials: M
authors.lastname: Hafizi
authors.affiliation: Stem Cell Technology Research Center, Tehran, Iran. athena.hajarizadeh@gmail.com.
authors.forename: Atena
authors.initials: A
authors.lastname: Hajarizadeh
authors.affiliation: Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. atashia@gmail.com.
authors.forename: Amir
authors.initials: A
authors.lastname: Atashi
authors.affiliation: Department of Research and Development, Sodour Ahrar Shargh Company, Tehran, Iran. skalanaky@nanochelatingtechnology.com.
authors.forename: Somayeh
authors.initials: S
authors.lastname: Kalanaky
authors.affiliation: Department of Research and Development, Sodour Ahrar Shargh Company, Tehran, Iran. sfakharzade@nanochelatingtechnology.com.
authors.forename: Saideh
authors.initials: S
authors.lastname: Fakharzadeh
authors.affiliation: Stem Cell Technology Research Center, Tehran, Iran. zahra.masoumi@yahoo.com.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Masoumi
authors.affiliation: Department of Research and Development, Sodour Ahrar Shargh Company, Tehran, Iran. mnazaran@nanochelatingtechnology.com.
authors.forename: Mohammad Hassan
authors.initials: MH
authors.lastname: Nazaran
authors.affiliation: Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. soleim_m@modares.ac.ir.
authors.forename: Masoud
authors.initials: M
authors.lastname: Soleimani
mesh_terms: D000943:Antigens, Differentiation / Q000096:biosynthesis; D000975:Antioxidants; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D049109:Cell Proliferation; D002470:Cell Survival; D002614:Chelating Agents; D003300:Copper; D003470:Culture Media; D006801:Humans; D007502:Iron Chelating Agents; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D054118:Nanospheres; D039904:Pluripotent Stem Cells / Q000166:cytology
chemical_list: D000943:Antigens, Differentiation; D000975:Antioxidants; D002614:Chelating Agents; D003470:Culture Media; D007502:Iron Chelating Agents; D003300:Copper