title: Human adipose stem cell and ASC-derived cardiac progenitor cellular therapy improves outcomes in a murine model of myocardial infarction.
abstract: BACKGROUND
  Adipose tissue is an abundant and potent source of adult stem cells for transplant therapy. In this study, we present our findings on the potential application of adipose-derived stem cells (ASCs) as well as induced cardiac-like progenitors (iCPs) derived from ASCs for the treatment of myocardial infarction.
  
  
  RESULTS
  Human bone marrow (BM)-derived stem cells, ASCs, and iCPs generated from ASCs using three defined cardiac lineage transcription factors were assessed in an immune-compromised mouse myocardial infarction model. Analysis of iCP prior to transplant confirmed changes in gene and protein expression consistent with a cardiac phenotype. Endpoint analysis was performed 1 month posttransplant. Significantly increased endpoint fractional shortening, as well as reduction in the infarct area at risk, was observed in recipients of iCPs as compared to the other recipient cohorts. Both recipients of iCPs and ASCs presented higher myocardial capillary densities than either recipients of BM-derived stem cells or the control cohort. Furthermore, mice receiving iCPs had a significantly higher cardiac retention of transplanted cells than all other groups.
  
  
  CONCLUSIONS
  Overall, iCPs generated from ASCs outperform BM-derived stem cells and ASCs in facilitating recovery from induced myocardial infarction in mice.
authors.affiliation: Institute for Biogenesis Research, University of Hawaii at Mānoa, Honolulu, HI, USA.
authors.forename: Philip Mc
authors.initials: PM
authors.lastname: Davy
authors.affiliation: John A. Burns School of Medicine, University of Hawaii at Mānoa, Honolulu, HI, USA ; Tissue Genesis, Inc., Honolulu, HI, USA.
authors.forename: Kevin D
authors.initials: KD
authors.lastname: Lye
authors.affiliation: Institute for Biogenesis Research, University of Hawaii at Mānoa, Honolulu, HI, USA.
authors.forename: Juanita
authors.initials: J
authors.lastname: Mathews
authors.affiliation: Institute for Biogenesis Research, University of Hawaii at Mānoa, Honolulu, HI, USA.
authors.forename: Jesse B
authors.initials: JB
authors.lastname: Owens
authors.affiliation: Institute for Biogenesis Research, University of Hawaii at Mānoa, Honolulu, HI, USA.
authors.forename: Alice Y
authors.initials: AY
authors.lastname: Chow
authors.affiliation: John A. Burns School of Medicine, University of Hawaii at Mānoa, Honolulu, HI, USA.
authors.forename: Livingston
authors.initials: L
authors.lastname: Wong
authors.affiliation: Institute for Biogenesis Research, University of Hawaii at Mānoa, Honolulu, HI, USA.
authors.forename: Stefan
authors.initials: S
authors.lastname: Moisyadi
authors.affiliation: Institute for Biogenesis Research, University of Hawaii at Mānoa, Honolulu, HI, USA.
authors.forename: Richard C
authors.initials: RC
authors.lastname: Allsopp
keywords: adipose stem cells; cellular reprogramming; cellular therapy; induced cardiac-like progenitors; myocardial infarction; piggyBac