title: Smilax glabra Roxb targets Akt(p-Thr308) and inhibits Akt-mediated signaling pathways in SGC7901 cells.
abstract: BACKGROUND
  Smilax glabra Roxb (SGR) as a novel anti-tumor agent has been paid attention in several types of cancer cells. However, the effect of SGR on SGC7901 cells has not been investigated.
  
  
  OBJECTIVE
  We investigate the effect and potential mechanisms of SGR on SGC7901 cells in this study.
  
  
  METHODS
  Three kinds of gastric cancer cell lines (BGC823, SGC7901 and MKN45) and one kind of human embryonic kidney cell line (HEK293) were exposed to varying concentrations of SRG. Then, we observed the effect of SRG on these cell lines and the changes on proliferation, invasion and apoptosis. Finally, we detected the signaling pathway in which SGR may involve.
  
  
  RESULTS
  SGR effectively suppressed the proliferation of SGC7901 cell lines by inhibiting the phosphorylation of Akt (Thr308). Moreover, we found SGR could significantly induce SGC7901 cell lines apoptosis by inhibiting Akt(p-Thr308)/Bad pathway and inhibit its migration and invasion partly by inhibiting Akt(p-Thr308)/MMPs pathway.
  
  
  CONCLUSIONS
  SGR could effectively suppress the proliferation and invasion of SGC7901 cell lines by inhibiting the phosphorylation of Akt (Thr308) and its downstream relative pathways.
  
  
  CONCLUSIONS
  SGR could effectively suppress the phosphorylation of Akt (Thr308) and then inhibit the proliferation and invasion of SGC7901 cell and enhance its apoptosis through Akt-mediated signaling pathways.
authors.affiliation: a Department of Neurosurgery , The Graduate Training Base of General Hospital of Shenyang Military Area Command, Liaoning Medical College , Shenyang , Liaoning , China .
authors.forename: Guangzhi
authors.initials: G
authors.lastname: Hao
authors.affiliation: b Department of Outpatient , The 463rd Hospital of PLA , Shenyang , Liaoning , China , and.
authors.forename: Jun
authors.initials: J
authors.lastname: Zheng
authors.affiliation: a Department of Neurosurgery , The Graduate Training Base of General Hospital of Shenyang Military Area Command, Liaoning Medical College , Shenyang , Liaoning , China .
authors.forename: Rentao
authors.initials: R
authors.lastname: Huo
authors.affiliation: c Department of Neurosurgery , General Hospital of Shenyang Military Area Command , Shenyang , Liaoning , China.
authors.forename: Jingchen
authors.initials: J
authors.lastname: Li
authors.affiliation: a Department of Neurosurgery , The Graduate Training Base of General Hospital of Shenyang Military Area Command, Liaoning Medical College , Shenyang , Liaoning , China .
authors.forename: Kai
authors.initials: K
authors.lastname: Wen
authors.affiliation: a Department of Neurosurgery , The Graduate Training Base of General Hospital of Shenyang Military Area Command, Liaoning Medical College , Shenyang , Liaoning , China .
authors.forename: Yinsong
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: c Department of Neurosurgery , General Hospital of Shenyang Military Area Command , Shenyang , Liaoning , China.
authors.forename: Guobiao
authors.initials: G
authors.lastname: Liang
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D057809:HEK293 Cells; D006801:Humans; D009361:Neoplasm Invasiveness / Q000517:prevention & control; D010766:Phosphorylation / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D032281:Smilax / Q000737:chemistry*; D013274:Stomach Neoplasms / Q000188:drug therapy* / Q000473:pathology
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts; D051057:Proto-Oncogene Proteins c-akt
keywords: Akt; Smilax glabra Roxb; gastric cancer cell; phosphorylation; signaling pathway