title: Variation in accumulation of isoflavonoids in Phaseoleae seedlings elicited by Rhizopus.
abstract: Seeds from seven species of tribe Phaseoleae, i.e. Phaseolus, Vigna, Lablab and Psophocarpus, were investigated for inducibility of isoflavonoids by germination with or without subsequent elicitation with Rhizopus oryzae. Germination alone poorly induced isoflavonoid production (in the range of 0.2-0.7 mg representative compound equivalents (RCE)/g DW), whereas application of Rhizopus onto the seedlings increased the isoflavonoid content considerably (in the range of 0.5-3.3 mg RCE/g DW). The inducibility of different isoflavonoid subclasses in seedlings with Rhizopus varied per species. Isoflavones and isoflavanones were mainly found in elicited seedlings of Phaseolus, Vigna and Lablab, whereas pterocarpans were mainly observed in those of Psophocarpus. Despite their phylogenetic relatedness, the seeds of various species within Phaseoleae appeared to respond differently towards elicitation by Rhizopus during germination. The kind of molecules induced followed the phylogenetic relationship of the various species, but their amounts induced during germination, alone or combined with elicitation, did not.
authors.affiliation: Laboratory of Food Chemistry, Wageningen University, P.O. Box 17, 6700 AA Wageningen, The Netherlands; Department of Chemistry Education, Indonesia University of Education, Setiabudi 229, Bandung 40154, Indonesia.
authors.forename: Siti
authors.initials: S
authors.lastname: Aisyah
authors.affiliation: Laboratory of Food Chemistry, Wageningen University, P.O. Box 17, 6700 AA Wageningen, The Netherlands.
authors.forename: Harry
authors.initials: H
authors.lastname: Gruppen
authors.affiliation: Laboratory of Food Chemistry, Wageningen University, P.O. Box 17, 6700 AA Wageningen, The Netherlands.
authors.forename: Silvia
authors.initials: S
authors.lastname: Andini
authors.affiliation: Laboratory of Food Chemistry, Wageningen University, P.O. Box 17, 6700 AA Wageningen, The Netherlands.
authors.forename: Monique
authors.initials: M
authors.lastname: Bettonvil
authors.affiliation: Laboratory of Genetics, Wageningen University, P.O. Box 16, 6700 AA Wageningen, The Netherlands.
authors.forename: Edouard
authors.initials: E
authors.lastname: Severing
authors.affiliation: Laboratory of Food Chemistry, Wageningen University, P.O. Box 17, 6700 AA Wageningen, The Netherlands. Electronic address: jean-paul.vincken@wur.nl.
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Vincken
mesh_terms: D018525:Germination; D007529:Isoflavones / Q000032:analysis / Q000378:metabolism*; D027805:Phaseolus / Q000737:chemistry* / Q000145:classification / Q000378:metabolism / Q000382:microbiology*; D010802:Phylogeny; D010936:Plant Extracts / Q000032:analysis* / Q000378:metabolism; D012233:Rhizopus / Q000378:metabolism*; D036226:Seedlings / Q000737:chemistry / Q000145:classification / Q000378:metabolism / Q000382:microbiology; D012639:Seeds / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism / Q000382:microbiology
chemical_list: D007529:Isoflavones; D010936:Plant Extracts
keywords: Flavonoid; Germination; Mass spectrometry; Phylogenetic relationship; Phytoalexin; Prenylation