title: In Vivo Targeted Magnetic Resonance Imaging of Endogenous Neural Stem Cells in the Adult Rodent Brain.
abstract: Neural stem cells in the adult mammalian brain have a significant level of neurogenesis plasticity. In vivo monitoring of adult endogenous NSCs would be of great benefit to the understanding of the neurogenesis plasticity under normal and pathological conditions. Here we show the feasibility of in vivo targeted MR imaging of endogenous NSCs in adult mouse brain by intraventricular delivery of monoclonal anti-CD15 antibody conjugated superparamagnetic iron oxide nanoparticles. After intraventricular administration of these nanoparticles, the subpopulation of NSCs in the anterior subventricular zone and the beginning of the rostral migratory stream could be in situ labeled and were in vivo visualized with 7.0-T MR imaging during a period from 1 day to 7 days after the injection. Histology confirmed that the injected targeted nanoparticles were specifically bound to CD15 positive cells and their surrounding extracellular matrix. Our results suggest that in vivo targeted MR imaging of endogenous neural stem cells in adult rodent brain could be achieved by using anti-CD15-SPIONs as the molecular probe; and this targeting imaging strategy has the advantage of a rapid in vivo monitoring of the subpopulation of endogenous NSCs in adult brains.
authors.affiliation: Department of Radiology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, No. 107 Yanjiang Road West, Guangzhou, Guangdong 510120, China.
authors.forename: Xiao-Mei
authors.initials: XM
authors.lastname: Zhong
authors.affiliation: Department of Radiology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, No. 107 Yanjiang Road West, Guangzhou, Guangdong 510120, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Department of Radiology, Zhongda Hospital of Southeast University, Nanjing 210009, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Yang
authors.affiliation: Department of Radiology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, No. 107 Yanjiang Road West, Guangzhou, Guangdong 510120, China.
authors.forename: Xue-Hua
authors.initials: XH
authors.lastname: Wen
authors.affiliation: Department of Radiology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, No. 107 Yanjiang Road West, Guangzhou, Guangdong 510120, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Radiology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, No. 107 Yanjiang Road West, Guangzhou, Guangdong 510120, China.
authors.forename: Xiao-Hui
authors.initials: XH
authors.lastname: Duan
authors.affiliation: Department of Radiology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, No. 107 Yanjiang Road West, Guangzhou, Guangdong 510120, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Shen
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage / Q000737:chemistry; D001921:Brain / Q000000981:diagnostic imaging* / Q000502:physiology; D003287:Contrast Media / Q000008:administration & dosage / Q000737:chemistry; D005647:Fucosyltransferases / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology; D007501:Iron / Q000737:chemistry; D008279:Magnetic Resonance Imaging; D051379:Mice; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry; D058953:Neural Stem Cells / Q000000981:diagnostic imaging* / Q000502:physiology; D055495:Neurogenesis; D009474:Neurons / Q000000981:diagnostic imaging*; D011859:Radiography
chemical_list: D000911:Antibodies, Monoclonal; D003287:Contrast Media; D007501:Iron; D005647:Fucosyltransferases; C541914:Fut4 protein, mouse