title: Phyllonema aviceniicolagen. nov., sp. nov. andFoliisarcina bertiogensisgen. nov., sp. nov., epiphyllic cyanobacteria associated withAvicennia schauerianaleaves.
abstract: Cyanobacteria dwelling on the salt-excreting leaves of the mangrove treeAvicennia schauerianawere isolated and characterized by ecological, morphological and genetic approaches. Leaves were collected in a mangrove with a history of oil contamination on the coastline of São Paulo state, Brazil, and isolation was achieved by smearing leaves on the surface of solid media or by submerging leaves in liquid media. Twenty-nine isolated strains were shown to belong to five cyanobacterial orders (thirteen toSynechococcales, seven toNostocales, seven toPleurocapsales, one toChroococcales, and one toOscillatoriales) according to morphological and 16S rRNA gene sequence evaluations. More detailed investigations pointed six Rivulariacean and four Xenococcacean strains as novel taxa. These strains were classified asPhyllonemagen. nov. (type speciesPhyllonema aviceniicolasp. nov. with type strain CENA341T) andFoliisarcinagen. nov. (type speciesFoliisarcina bertiogensissp. nov. with type strain CENA333T), according to the International Code of Nomenclature for Algae, Fungi, and Plants. This investigation shows some of the unique cyanobacteria inhabiting the phyllosphere ofAvicennia schauerianacan be retrieved by culturing techniques, improving current taxonomy and providing new insights into the evolution, ecology, and biogeography of this phylum.
authors.affiliation: University of São Paulo, Center for Nuclear Energy in Agriculture, Avenida Centenário 303, 13400-970 Piracicaba, SP, Brazil.
authors.forename: Danillo
authors.initials: D
authors.lastname: Oliveira Alvarenga
authors.affiliation: University of São Paulo, Center for Nuclear Energy in Agriculture, Avenida Centenário 303, 13400-970 Piracicaba, SP, Brazil.
authors.forename: Janaina
authors.initials: J
authors.lastname: Rigonato
authors.affiliation: São Paulo State University, Institute of Bioscience, Languages and Exact Sciences, 15054-000 São José do Rio Preto, SP, Brazil.
authors.forename: Luis
authors.initials: L
authors.lastname: Henrique Zanini Branco
authors.affiliation: Embrapa Environment, Laboratory of Environmental Microbiology, 13820-000 Jaguariúna, SP, Brazil.
authors.forename: Itamar
authors.initials: I
authors.lastname: Soares Melo
authors.affiliation: University of São Paulo, Center for Nuclear Energy in Agriculture, Avenida Centenário 303, 13400-970 Piracicaba, SP, Brazil.
authors.forename: Marli
authors.initials: M
authors.lastname: Fatima Fiore