title: Utilization of Site-Specific Recombination in Biopharmaceutical Production.
abstract: Mammalian expression systems, due to their capacity in post-translational modification, are preferred systems for biopharmaceutical protein production. Several recombinant protein systems have been introduced to the market, most of which are under clinical development. In spite of significant improvements such as cell line engineering, introducing novel expression methods, gene silencing and process development, expression level is unpredictable and unstable because of the random location of integration in the genome. Site-specific recombination techniques are capable of producing stable and high producer clonal cells; therefore, they are gaining more importance in the biopharmaceutical production. Site-specific recombination methods increase the recombinant protein production by specifically inserting a vector at a locus with specific expression trait. The present review focused on the latest developments in site-specific recombination techniques, their specific features and comparisons.
authors.affiliation: Dept. of Medical Biotechnology, Semnan University of Medical Sciences, Semnan, Iran.|Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Ahmadi
authors.affiliation: Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran.|Dept. of Biotechnology, College of Science, University of Tehran, Tehran, Iran.
authors.forename: Narges
authors.initials: N
authors.lastname: Damavandi
authors.affiliation: Dept. of Medical Biotechnology, Semnan University of Medical Sciences, Semnan, Iran.
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Akbari Eidgahi
authors.affiliation: Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Davami
mesh_terms: D000818:Animals; D001702:Biopharmaceutics / Q000379:methods*; D022222:Chromosomes, Artificial, Human / Q000235:genetics / Q000378:metabolism; D064112:Clustered Regularly Interspaced Short Palindromic Repeats / Q000235:genetics; D005818:Genetic Engineering / Q000379:methods*; D006801:Humans; D011994:Recombinant Proteins / Q000235:genetics* / Q000378:metabolism; D011995:Recombination, Genetic / Q000235:genetics*
chemical_list: D011994:Recombinant Proteins
keywords: CHO cell; Gene targeting; Site-specific recombinase