title: [Study on contribution of main components in Guizhi Fuling capsule based on molecular imprinting technique and activity screening].
abstract: To clarify the active components in Guizhi Fuling capsule in treatment of intrinsic dysmenorrhea, pelvic inflammation and hysteromyoma, main components were gradually knocked out from the capsules, the effects of knockout capsules on uterine contraction, TNF-α secretion, murine splenocytes (SPL) and hysteromyoma cells proliferation were evaluated, respectively. The inhibition of capsules on uterine contraction was weakened by gradient knockout of paeoniflorin, paeonol, and amygdalin. The suppression of capsulte on TNF-α secretion was reduced by gradient knockout of gallic acid, cinnamaldehyde, pentagalloylglucose, and pachyman. The promotion of SPL cells proliferation was reversed by gradient knockout of gallic acid, paeoniflorin, cinnamaldehyde, quercetin, and pachyman. The depression of capsules on hysteromyoma cells proliferation was attenuated by gradient knockout of paeoniflorin, paeonol, pentagalloylglucose, and albiflorin. In conclusion, the compounds mentioned-above could be the key active basis of Guizhi Fuling capsule in treatment of intrinsic dysmenorrhea, pelvic inflammation and hysteromyoma.
authors.forename: Ze-yu
authors.initials: ZY
authors.lastname: Cao
authors.forename: Yue
authors.initials: Y
authors.lastname: Ding
authors.forename: Zhen-zhen
authors.initials: ZZ
authors.lastname: Su
authors.forename: Na
authors.initials: N
authors.lastname: Li
authors.forename: Liabg
authors.initials: L
authors.lastname: Cao
authors.forename: Gang
authors.initials: G
authors.lastname: Ding
authors.forename: Zhen-zhong
authors.initials: ZZ
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Xiao
mesh_terms: D000818:Animals; D002214:Capsules / Q000008:administration & dosage / Q000737:chemistry; D049109:Cell Proliferation / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage* / Q000737:chemistry*; D004412:Dysmenorrhea / Q000188:drug therapy* / Q000378:metabolism / Q000503:physiopathology; D005260:Female; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D054802:Molecular Imprinting / Q000379:methods; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D002214:Capsules; D004365:Drugs, Chinese Herbal; D014409:Tumor Necrosis Factor-alpha