title: The rs5934505 single nucleotide polymorphism (SNP) is associated with low testosterone and late-onset hypogonadism, but the rs10822184 SNP is associated with overweight and obesity in a Chinese Han population: a case-control study.
abstract: Low testosterone is associated with late-onset hypogonadism (LOH) and obesity. Recently, studies have shown that four single nucleotide polymorphisms (SNPs), rs12150660, rs727428, rs5934505, and rs10822184, are associated with testosterone levels in populations of European descent. Therefore, we investigated whether the SNP loci are related to low testosterone, LOH, or obesity in a Chinese Han population. Ruling out co-morbidities, DNA was prepared from 409 men (aged 40-65 years) with low serum testosterone (defined as total testosterone <11.6 nmol/L) and 1 : 1 normal controls (matched age, body mass index (BMI), and the same living area) who were selected from 6898 males. According to the same standards, 310 men with LOH and 1 : 1 normal controls were selected from 6898 males. Excluding the cases with an unreliable sequencing result, genetic analyses were performed. The minor allele frequencies of the SNP loci rs12150660, rs727428, rs5934505, and rs10822184 were 0.1%, 44.6%, 18.7%, and 38.9%, respectively. rs5934505 was associated with the serum total testosterone and calculated free testosterone (CFT) levels (p = 0.045 and p = 0.021). rs5934505 (C>T) was associated with an increased risk of low total testosterone, low CFT, and LOH and adjusted for other factors, with an odds ratio (OR) of 2.01 (1.34-3.01), 2.14 (1.42-3.20), and 1.64 (1.04-2.58). rs10822184 was significantly correlated with weight and BMI (p = 0.035 and p = 0.027). rs10822184 (T>C) was associated with an increased risk of overweight and obesity. We adjusted for other factors, with odds ratios (ORs) of 1.94 (1.36-2.78) and 1.56 (1.00-2.43). In summary, our study provided convincing evidence that rs5934505 (C>T) was associated with the risk of low testosterone and LOH in Chinese populations. We were the first to find that rs10822184 (T>C) was significantly correlated with the risk of overweight and obesity in Chinese populations. However, further large and functional studies are warranted to confirm our findings.
authors.affiliation: Family Planning Research Institute/Center of Reproductive Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: Y-P
authors.initials: YP
authors.lastname: Chen
authors.affiliation: Medical Laboratory, Shenzhen Research Institute of Population and Family Planning, Futian, Shenzhen, China.
authors.forename: L-L
authors.initials: LL
authors.lastname: Nie
authors.affiliation: Family Planning Research Institute/Center of Reproductive Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.|Wuhan Tongji Reproductive Medicine Hospital, Wuhan, China.
authors.forename: H-G
authors.initials: HG
authors.lastname: Li
authors.affiliation: Family Planning Research Institute/Center of Reproductive Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: T-H
authors.initials: TH
authors.lastname: Liu
authors.affiliation: Family Planning Research Institute/Center of Reproductive Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: F
authors.initials: F
authors.lastname: Fang
authors.affiliation: Family Planning Research Institute/Center of Reproductive Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: K
authors.initials: K
authors.lastname: Zhao
authors.affiliation: Wuhan Tongji Reproductive Medicine Hospital, Wuhan, China.
authors.forename: R-F
authors.initials: RF
authors.lastname: Yang
authors.affiliation: Family Planning Research Institute/Center of Reproductive Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: X-L
authors.initials: XL
authors.lastname: Ma
authors.affiliation: Family Planning Research Institute/Center of Reproductive Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: X-B
authors.initials: XB
authors.lastname: Kong
authors.affiliation: Family Planning Research Institute/Center of Reproductive Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.|Wuhan Tongji Reproductive Medicine Hospital, Wuhan, China.
authors.forename: H-P
authors.initials: HP
authors.lastname: Zhang
authors.affiliation: Family Planning Research Institute/Center of Reproductive Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.|Wuhan Tongji Reproductive Medicine Hospital, Wuhan, China.
authors.forename: H-T
authors.initials: HT
authors.lastname: Guan
authors.affiliation: Family Planning Research Institute/Center of Reproductive Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: W
authors.initials: W
authors.lastname: Xia
authors.affiliation: Medical Laboratory, Shenzhen Research Institute of Population and Family Planning, Futian, Shenzhen, China.
authors.forename: W-X
authors.initials: WX
authors.lastname: Hong
authors.affiliation: Medical Laboratory, Shenzhen Research Institute of Population and Family Planning, Futian, Shenzhen, China.
authors.forename: S
authors.initials: S
authors.lastname: Duan
authors.affiliation: Medical Laboratory, Shenzhen Research Institute of Population and Family Planning, Futian, Shenzhen, China.
authors.forename: X-C
authors.initials: XC
authors.lastname: Zeng
authors.affiliation: Department of Andrology, Jinling Hospital, School of Medicine, Nanjing University, Nanjing, China.
authors.forename: X-J
authors.initials: XJ
authors.lastname: Shang
authors.affiliation: School of Public Health, Zunyi Medical University, Zunyi, Guizhou, China.
authors.forename: Y-Z
authors.initials: YZ
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Male Reproductive Health, National Health and Family Planning Commission, National Research Institute for Family Planning, Hai Dian, Beijing, China.
authors.forename: Y-Q
authors.initials: YQ
authors.lastname: Gu
authors.affiliation: Guangzhou Institute for Population and Family Planning, Baiyun, Guangzhou, China.
authors.forename: W-X
authors.initials: WX
authors.lastname: Wu
authors.affiliation: Family Planning Research Institute/Center of Reproductive Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.|Wuhan Tongji Reproductive Medicine Hospital, Wuhan, China.
authors.forename: C-L
authors.initials: CL
authors.lastname: Xiong
mesh_terms: D000328:Adult; D000368:Aged; D001835:Body Weight / Q000235:genetics*; D016022:Case-Control Studies; D002681:China / Q000453:epidemiology; D005787:Gene Frequency / Q000235:genetics; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D006304:Health Status; D006801:Humans; D007006:Hypogonadism / Q000453:epidemiology / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000453:epidemiology / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011795:Surveys and Questionnaires; D013739:Testosterone / Q000097:blood*
chemical_list: D013739:Testosterone
keywords: late-onset hypogonadism; obesity; rs10822184; rs5934505; single nucleotide polymorphism; testosterone