title: Staphylokinase and ABO group phenotype: new players in Staphylococcus aureus implant-associated infections development.
abstract: OBJECTIVE
  To identify bacterial and/or clinical features involved in the pathogenesis of Staphylococcus aureus implant-associated infections (IAI).
  
  
  METHODS
  In total, 57 IAI S. aureus and 31 nasal carriage (NC) S. aureus isolates were studied. Staphylococcus aureus genetic background was obtained by microarray analysis. Multilocus sequence typing was performed to determine clonal complexes (CC). Biofilm production was investigated by resazurin and crystal violet methods.
  
  
  RESULTS
  Staphylokinase gene was associated with the occurrence of S. aureus IAI. Patients' ABO blood group phenotype was associated with IAI S. aureus genetic background. CC8 S. aureus strains produce more biofilm than others and carry particular alleles of bbp gene.
  
  
  CONCLUSIONS
  This study identifies some predictive markers for S. aureus IAI.
authors.affiliation: Laboratoire UPRES EA3826, Thérapeutiques cliniques et expérimentales des infections - Nantes Medicine school, France.|Service de Bactériologie-Hygiène hospitalière, Nantes University Hospital, France.
authors.forename: Guillaume Ghislain
authors.initials: GG
authors.lastname: Aubin
authors.affiliation: INSERM U1047, Université de Montpellier, Nîmes, France.|Service de Microbiologie, CHU Carémeau, Nîmes University Hospital, Nîmes, France.
authors.forename: Jean-Philippe
authors.initials: JP
authors.lastname: Lavigne
authors.affiliation: INSERM/Unité Mixte de Recherche (UMR) 1087, Institut du Thorax, Nantes, France.
authors.forename: Béatrice
authors.initials: B
authors.lastname: Guyomarch
authors.affiliation: INSERM/Unité Mixte de Recherche (UMR) 1087, Institut du Thorax, Nantes, France.
authors.forename: Christian
authors.initials: C
authors.lastname: Dina
authors.affiliation: Clinique chirurgicale orthopédique et traumatique, Nantes University Hospital, France.
authors.forename: François
authors.initials: F
authors.lastname: Gouin
authors.affiliation: Laboratoire UPRES EA3826, Thérapeutiques cliniques et expérimentales des infections - Nantes Medicine school, France.|Service de Bactériologie-Hygiène hospitalière, Nantes University Hospital, France.
authors.forename: Didier
authors.initials: D
authors.lastname: Lepelletier
authors.affiliation: Laboratoire UPRES EA3826, Thérapeutiques cliniques et expérimentales des infections - Nantes Medicine school, France.|Service de Bactériologie-Hygiène hospitalière, Nantes University Hospital, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Corvec
mesh_terms: D000017:ABO Blood-Group System / Q000378:metabolism*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D005838:Genotype; D054884:Host-Pathogen Interactions; D006801:Humans; D008297:Male; D008666:Metalloendopeptidases / Q000378:metabolism*; D046228:Microarray Analysis; D008875:Middle Aged; D058885:Multilocus Sequence Typing; D010641:Phenotype; D016459:Prosthesis-Related Infections / Q000382:microbiology*; D013203:Staphylococcal Infections / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000201:enzymology / Q000302:isolation & purification* / Q000472:pathogenicity; D055815:Young Adult
chemical_list: D000017:ABO Blood-Group System; D008666:Metalloendopeptidases; C022653:auR protein, Staphylococcus aureus
keywords: Staphylococcus aureus; biofilm; blood group; bone and joint infection; bone binding sialoprotein; microarrays; staphylokinase