title: Effect of Low-Magnitude, High-Frequency Vibration Treatment on Retardation of Sarcopenia: Senescence-Accelerated Mouse-P8 Model.
abstract: Sarcopenia-related falls and fall-related injuries in community-dwelling elderly people garnered more and more interest in recent years. Low-magnitude high-frequency vibration (LMHFV) was proven beneficial to musculoskeletal system and recommended for sarcopenia treatment. This study aimed to evaluate the effects of LMHFV on the sarcopenic animals and explore the mechanism of the stimulatory effects. Senescence-accelerated mouse P8 (SAMP8) mice at month 6 were randomized into control (Ctrl) and vibration (Vib) groups and the mice in the Vib group were given LMHFV (0.3 g, 20 min/day, 5 days/week) treatment. At months 0, 1, 2, 3, and 4 post-treatment, muscle mass, structure, and function were assessed. The potential proliferation capacity of the muscle was also evaluated by investigating satellite cells (SCs) pool and serum myostatin expression. At late stage, the mice in the Vib group showed higher muscle strength (month 4, p = 0.028). Generally, contractibility was significantly improved by LMHFV (contraction time [CT], p = 0.000; half-relaxation time [RT50], p = 0.000). Enlarged cross-sectional area of fiber type IIA was observed in the Vib group when compared with Ctrl group (p = 0.000). No significant difference of muscle mass was observed. The promotive effect of LMHFV on myoregeneration was reflected by suppressed SC pool reduction (month 3, p = 0.000; month 4, p = 0.000) and low myostatin expression (p = 0.052). LMHFV significantly improved the structural and functional outcomes of the skeletal muscle, hence retarding the progress of sarcopenia in SAMP8. It would be a good recommendation for prevention of the diseases related to skeletal muscle atrophy.
authors.affiliation: 1 Department of Orthopaedics and Traumatology, Prince of Wales Hospital, The Chinese University of Hong Kong , Hong Kong SAR, China .
authors.forename: An-Yun
authors.initials: AY
authors.lastname: Guo
authors.affiliation: 1 Department of Orthopaedics and Traumatology, Prince of Wales Hospital, The Chinese University of Hong Kong , Hong Kong SAR, China .|2 Translational Medicine Research & Development Center, Institute of Biomedical and Health Engineering, Shenzhen Institutes of Advanced Technology , Chinese Academy of Sciences, Beijing, China .
authors.forename: Kwok-Sui
authors.initials: KS
authors.lastname: Leung
authors.affiliation: 1 Department of Orthopaedics and Traumatology, Prince of Wales Hospital, The Chinese University of Hong Kong , Hong Kong SAR, China .
authors.forename: Jiang-Hui
authors.initials: JH
authors.lastname: Qin
authors.affiliation: 1 Department of Orthopaedics and Traumatology, Prince of Wales Hospital, The Chinese University of Hong Kong , Hong Kong SAR, China .
authors.forename: Simon Kwoon-Ho
authors.initials: SK
authors.lastname: Chow
authors.affiliation: 1 Department of Orthopaedics and Traumatology, Prince of Wales Hospital, The Chinese University of Hong Kong , Hong Kong SAR, China .|2 Translational Medicine Research & Development Center, Institute of Biomedical and Health Engineering, Shenzhen Institutes of Advanced Technology , Chinese Academy of Sciences, Beijing, China .|3 The CUHK-ACC Space Medicine Centre on Health Maintenance of Musculoskeletal System, The Chinese University of Hong Kong Shenzhen Research Institute , Shenzhen, China .
authors.forename: Wing-Hoi
authors.initials: WH
authors.lastname: Cheung
mesh_terms: D000367:Age Factors; D000375:Aging; D000818:Animals; D049109:Cell Proliferation; D004195:Disease Models, Animal; D008297:Male; D051379:Mice; D008840:Microfilament Proteins; D009119:Muscle Contraction; D024510:Muscle Development; D018763:Muscle Fatigue; D009124:Muscle Proteins / Q000097:blood; D053580:Muscle Strength; D018482:Muscle, Skeletal / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D055948:Sarcopenia / Q000097:blood / Q000473:pathology / Q000503:physiopathology / Q000517:prevention & control*; D032496:Satellite Cells, Skeletal Muscle / Q000378:metabolism / Q000473:pathology; D013997:Time Factors; D014732:Vibration / Q000627:therapeutic use*
chemical_list: D008840:Microfilament Proteins; D009124:Muscle Proteins; C503771:Myot protein, mouse