title: Increased Rat Placental Fatty Acid, but Decreased Amino Acid and Glucose Transporters Potentially Modify Intrauterine Programming.
abstract: Regulation of placental nutrient transport significantly affects fetal development and may modify intrauterine growth restriction (IUGR) and fetal programming. We hypothesized that placental nutrient transporters are differentially affected both by utero-placental insufficiency and prenatal surgical stress. Pregnant rats underwent bilateral uterine artery and vein ligation (LIG), sham operation (SOP) or no operation (controls, C) on gestational day E19. Placentas were obtained by caesarean section 4 h (LIG, n=20 placentas; SOP, n=24; C, n=12), 24 h (LIG, n=28; SOP, n=20; C, n=12) and 72 h (LIG, n=20; SOP, n=20; C, n=24) after surgery. Gene and protein expression of placental nutrient transporters for fatty acids (h-FABP, CD36), amino acids (SNAT1, SNAT2) and glucose (GLUT-1, Connexin 26) were examined by qRT-PCR, western blot and immunohistochemistry. Interestingly, the mean protein expression of h-FABP was doubled in placentas of LIG and SOP animals 4, 24 (SOP significant) and 72 h (SOP significant) after surgery. CD36 protein was significantly increased in LIG after 72 h. SNAT1 and SNAT2 protein and gene expressions were significantly reduced in LIG and SOP after 24 h. Further significantly reduced proteins were GLUT-1 in LIG (4 h, 72 h) and SOP (24 h), and Connexin 26 in LIG (72 h). In conclusion, placental nutrient transporters are differentially affected both by reduced blood flow and stress, probably modifying the already disturbed intrauterine milieu and contributing to IUGR and fetal programming. Increased fatty acid transport capacity may affect energy metabolism and could be a compensatory reaction with positive effects on brain development. J. Cell. Biochem. 117: 1594-1603, 2016. © 2015 Wiley Periodicals, Inc.
authors.affiliation: Department of Pediatrics, Medical Faculty, University of Cologne, Cologne, Germany.|Department of Pediatrics, University of Erlangen-Nuremberg, Erlangen, Germany.
authors.forename: Eva
authors.initials: E
authors.lastname: Nüsken
authors.affiliation: Institute of Molecular Biology, University of Duisburg-Essen, Essen, Germany.|Department of Gynecology and Obstetrics, University Hospital Essen, Essen, Germany.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Gellhaus
authors.affiliation: Institute of Molecular Biology, University of Duisburg-Essen, Essen, Germany.|Department of Gynecology and Obstetrics, University Hospital Essen, Essen, Germany.
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Kühnel
authors.affiliation: Department of Pediatrics, Medical Faculty, University of Cologne, Cologne, Germany.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Swoboda
authors.affiliation: Department of Pediatrics, Medical Faculty, University of Cologne, Cologne, Germany.
authors.forename: Maria
authors.initials: M
authors.lastname: Wohlfarth
authors.affiliation: Department of Pediatrics, Medical Faculty, University of Cologne, Cologne, Germany.
authors.forename: Christina
authors.initials: C
authors.lastname: Vohlen
authors.affiliation: Department of Pediatrics, University of Erlangen-Nuremberg, Erlangen, Germany.
authors.forename: Holm
authors.initials: H
authors.lastname: Schneider
authors.affiliation: Department of Pediatrics, Medical Faculty, University of Cologne, Cologne, Germany.|Department of Pediatrics, University of Erlangen-Nuremberg, Erlangen, Germany.
authors.forename: Jörg
authors.initials: J
authors.lastname: Dötsch
authors.affiliation: Department of Pediatrics, Medical Faculty, University of Cologne, Cologne, Germany.|Department of Pediatrics, University of Erlangen-Nuremberg, Erlangen, Germany.
authors.forename: Kai-Dietrich
authors.initials: KD
authors.lastname: Nüsken
mesh_terms: D026924:Amino Acid Transport System A / Q000378:metabolism*; D026905:Amino Acid Transport Systems / Q000378:metabolism*; D000818:Animals; D018955:CD36 Antigens / Q000378:metabolism*; D000072259:Connexin 26; D017630:Connexins / Q000378:metabolism*; D000074026:Fatty Acid Binding Protein 3; D050556:Fatty Acid-Binding Proteins / Q000378:metabolism*; D005260:Female; D051272:Glucose Transporter Type 1 / Q000378:metabolism*; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar
chemical_list: D026924:Amino Acid Transport System A; D026905:Amino Acid Transport Systems; D018955:CD36 Antigens; D017630:Connexins; C509948:FABP3 protein, rat; D000074026:Fatty Acid Binding Protein 3; D050556:Fatty Acid-Binding Proteins; C000607044:Gjb2 protein, rat; D051272:Glucose Transporter Type 1; C495679:Slc2a1 protein, rat; C486716:Slc38a1 protein, rat; C412568:Slc38a2 protein, rat; D000072259:Connexin 26
keywords: DEVELOPMENT; FETUS; INTRAUTERINE GROWTH RESTRICTION; LIGATION; NUTRIENT TRANSPORTER; PLACENTA