title: Hematotoxic and biochemical effects of UVA on the Egyptian toad (Bufo regularis).
abstract: OBJECTIVE
  To study the adverse impacts of ultraviolet radiation-A (UVA 320-400 nm) on some hematological and biochemical parameters of Bufo regularis was considered.
  
  
  METHODS
  Samples were classified into four groups: (i) Control; (ii) ultraviolet radiation (UVR)-treated group (for 3 days/for 15 min/day); (iii) UVR-treated group (for 3 days/for 30 min/day); and (iv) (for 3 days/for 60 min/day). The destructive effects of UVA radiation was evaluated by red blood cells (RBC) count, hemoglobin content (Hb), hematocrite (Ht), erythrocytic indices, white blood cells (WBC) count, total protein, glucose, aspartic amino transferase (AST), alanine amino transferase (ALT), alkaline phosphatase (ALP), lactate dehyderogenase (LDH), glucose-6-phosphate dehyderogenase (G6PDH) and total bilribuin.
  
  
  RESULTS
  No mortality was observed. However, some physiological effects after the exposure to UVA were reported. The UVA-induced malformations recorded in the red blood cells included crenated cells (Cr), Acanthocytes (Ac), tear drop-like cells (Tr) and sickle cells (Sk).
  
  
  CONCLUSIONS
  The present study revealed the exposure to UVA from 15-60 min/day for three days could promote several biochemical and physiological disturbances as well as some changes in RBC.
authors.affiliation: a Zoology Department , Faculty of Science, Assiut University , Assiut , Egypt.
authors.forename: Alaa El-Din H
authors.initials: Ael-D
authors.lastname: Sayed
mesh_terms: D000818:Animals; D002025:Bufonidae / Q000502:physiology*; D002478:Cells, Cultured; D004307:Dose-Response Relationship, Radiation; D004913:Erythrocytes, Abnormal / Q000502:physiology* / Q000528:radiation effects*; D006410:Hematopoiesis / Q000502:physiology* / Q000528:radiation effects*; D011829:Radiation Dosage; D015996:Survival Rate; D014466:Ultraviolet Rays / Q000009:adverse effects*
keywords: Bufo regularis; UVA; biochemical; decline; erythrocytes