title: Effect of 10 different polymorphisms on preoperative volumetric characteristics of glioblastoma multiforme.
abstract: There is a distinct diversity between the appearance of every glioblastoma multiforme (GBM) on pretreatment magnetic resonance imaging (MRI) with a potential impact on clinical outcome and survival of the patients. The object of this study was to determine the impact of 10 different single nucleotide polymorphisms (SNPs) on various volumetric parameters in patients harboring a GBM. We prospectively analyzed 20 steroid-naïve adult patients who had been treated for newly diagnosed GBM. The volumetry was performed using MRI with the help of a semiautomated quantitative software measuring contrast enhancing tumor volume including necrosis, central necrosis alone and peritumoral edema (PTE). We calculated ratios between the tumor volume and edema (ETR), respectively necrosis (NTR). SNP analysis was done using genomic DNA extracted from peripheral blood genotyped via PCR and sequencing. There was a strong correlation between tumor volume and PTE (p < 0.001), necrosis (p < 0.001) and NTR (p = 0.003). Age and sex had no influence on volumetric data. The Aquaporin 4-31G > A SNP had a significant influence on the ETR (p = 0.042) by decreasing the measured edema compared with the tumor volume. The Interleukin 8-251A > T SNP was significantly correlated with an increased tumor (p = 0.048), PTE (p = 0.033) and necrosis volume (p = 0.028). We found two SNPs with a distinct impact on pretreatment tumor characteristics, presenting a potential explanation for the individual diversity of GBM appearance on MRI and influence on survival.
authors.affiliation: Department of Neurosurgery, University Hospital Rostock, Schillingallee 35, 18057, Rostock, Germany. christian.henker@med.uni-rostock.de.
authors.forename: Christian
authors.identifier: http://orcid.org/0000-0002-7591-3192
authors.initials: C
authors.lastname: Henker
authors.affiliation: Department of Neurosurgery, University Hospital Rostock, Schillingallee 35, 18057, Rostock, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Kriesen
authors.affiliation: Institute of Experimental Gene Therapy and Cancer Research, Rostock University Medical Center, Schillingallee 69, 18057, Rostock, Germany.
authors.forename: Katharina
authors.initials: K
authors.lastname: Fürst
authors.affiliation: Institute of Experimental Gene Therapy and Cancer Research, Rostock University Medical Center, Schillingallee 69, 18057, Rostock, Germany.
authors.forename: Deborah
authors.initials: D
authors.lastname: Goody
authors.affiliation: Institute for Biostatistics and Informatics in Medicine, Rostock University Medical Center, Ernst-Heydemann-Str. 8, 18057, Rostock, Germany.
authors.forename: Änne
authors.initials: Ä
authors.lastname: Glass
authors.affiliation: Institute of Experimental Gene Therapy and Cancer Research, Rostock University Medical Center, Schillingallee 69, 18057, Rostock, Germany.
authors.forename: Brigitte M
authors.initials: BM
authors.lastname: Pützer
authors.affiliation: Department of Neurosurgery, University Hospital Rostock, Schillingallee 35, 18057, Rostock, Germany.
authors.forename: Jürgen
authors.initials: J
authors.lastname: Piek
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000235:genetics*; D001932:Brain Neoplasms / Q000235:genetics* / Q000473:pathology* / Q000601:surgery; D005260:Female; D005500:Follow-Up Studies; D005909:Glioblastoma / Q000235:genetics* / Q000473:pathology* / Q000601:surgery; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000379:methods; D008297:Male; D008875:Middle Aged; D009336:Necrosis; D060787:Neoplasm Grading; D011110:Polymorphism, Genetic / Q000235:genetics*; D011300:Preoperative Care; D011379:Prognosis; D011446:Prospective Studies; D047368:Tumor Burden
chemical_list: D014408:Biomarkers, Tumor
keywords: Edema; Glioblastoma; Necrosis; Polymorphism; Volumetrics