title: Comparative analysis of chemical compositions between non-transgenic soybean seeds and those from plants over-expressing AtJMT, the gene for jasmonic acid carboxyl methyltransferase.
abstract: Transgenic overexpression of the Arabidopsis gene for jasmonic acid carboxyl methyltransferase (AtJMT) is involved in regulating jasmonate-related plant responses. To examine its role in the compositional profile of soybean (Glycine max), we compared the seeds from field-grown plants that over-express AtJMT with those of the non-transgenic, wild-type (WT) counterpart. Our analysis of chemical compositions included proximates, amino acids, fatty acids, isoflavones, and antinutrients. Overexpression of AtJMT in the seeds resulted in decreased amounts of tryptophan, palmitic acid, linolenic acid, and stachyose, but increased levels of gadoleic acid and genistein. In particular, seeds from the transgenic soybeans contained 120.0-130.5% more genistein and 60.5-82.1% less stachyose than the WT. A separate evaluation of ingredient values showed that all were within the reference ranges reported for commercially available soybeans, thereby demonstrating the substantial equivalence of these transgenic and non-transgenic seeds.
authors.affiliation: Bio-Evaluation Center, KRIBB, Cheongju 363-883, Republic of Korea.
authors.forename: Kyong-Hee
authors.initials: KH
authors.lastname: Nam
authors.affiliation: Bio-Evaluation Center, KRIBB, Cheongju 363-883, Republic of Korea.
authors.forename: Do Young
authors.initials: DY
authors.lastname: Kim
authors.affiliation: Bio-Evaluation Center, KRIBB, Cheongju 363-883, Republic of Korea.
authors.forename: In-Soon
authors.initials: IS
authors.lastname: Pack
authors.affiliation: Bio-Evaluation Center, KRIBB, Cheongju 363-883, Republic of Korea.
authors.forename: Jung-Ho
authors.initials: JH
authors.lastname: Park
authors.affiliation: Department of Agricultural Biotechnology, Seoul National University, 151-921, Republic of Korea.
authors.forename: Jun Sung
authors.initials: JS
authors.lastname: Seo
authors.affiliation: Department of Agricultural Biotechnology, Seoul National University, 151-921, Republic of Korea.
authors.forename: Yang Do
authors.initials: YD
authors.lastname: Choi
authors.affiliation: Center for Food and Bioconvergence, Seoul National University, Seoul 151-921, Republic of Korea.
authors.forename: Jong-Joo
authors.initials: JJ
authors.lastname: Cheong
authors.affiliation: Department of Food and Nutrition, Seowon University, Cheongju 361-742, Republic of Korea.
authors.forename: Chung Ho
authors.initials: CH
authors.lastname: Kim
authors.affiliation: Bio-Evaluation Center, KRIBB, Cheongju 363-883, Republic of Korea. Electronic address: cgkim@kribb.re.kr.
authors.forename: Chang-Gi
authors.initials: CG
authors.lastname: Kim
mesh_terms: D008780:Methyltransferases / Q000737:chemistry* / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry*; D013025:Glycine max / Q000737:chemistry*
chemical_list: D008780:Methyltransferases; C425043:jasmonic acid carboxyl methyltransferase
keywords: Chemical composition; Jasmonic acid; Jasmonic acid carboxyl methyltransferase; Methyl jasmonate; Soybean (Glycine max L.)