title: Variants of the Solute Carrier SLC16A1 Gene (MCT1) Associated With Metabolic Responses During a Long-Graded Test in Road Cyclists.
abstract: Variants of the solute carrier SLC16A1 gene have been associated with alterations in MCT1 expression, because of a lactate (La) transport deficiency across the cell membrane and a blood La accumulation. The aim of this study was to associate the allelic and genotypic frequencies of 1470T>A, 2917(1414) C>T, and IVS3-17A>C variants relative to the blood La kinetics and metabolic responses to a progressive effort until exhaustion. Twenty-five well-trained road cyclists performed a long-graded laboratory test: 10 minutes at 2.0 W·kg, first step at 2.5 W·kg with increments of 0.5 W·kg every 10 minutes until exhaustion. Blood La, nonesterified fatty acids (NEFAS), and glucose levels were measured; fat and carbohydrate oxidation rates were estimated through stoichiometric equations. Three variants of SLC16A1 gene were determined for each subject, which were divided in two groups: wt (wild type)/mt (mutated type) and mt/mt genotype group versus wt/wt genotype group. Metabolic responses were compared between both groups with an unpaired Student's t-test; Friedman and Wilcoxon tests were performed for nonparametric data. The statistical significance was set at p ≤ 0.05. For 1470TA polymorphism, no significant blood La differences were found between groups. 2197(1414)C>T allele carriers and IVS3-17A>C carriers showed significantly higher blood La levels, lower blood NEFAS, and glucose levels at submaximal intensities. These findings open a new perspective to investigate SLC16A1 variants (1470TA and IVS3-17A>C) on La deficiency transport and its regulation/interaction with other metabolic pathways. Future studies would be needed to clarify whether 1470T>A, 2917(1414)C>T, and IVS3-17A>C allelic/genotypic distribution benefit performance in endurance athletes.
authors.affiliation: Department Pharmacology and Physiology, School of Medicine, University of Zaragoza, Zaragoza, Spain.
authors.forename: Carlos
authors.initials: C
authors.lastname: González-Haro
authors.forename: Marisol
authors.initials: M
authors.lastname: Soria
authors.forename: Jorge
authors.initials: J
authors.lastname: Vicente
authors.forename: Ana J
authors.initials: AJ
authors.lastname: Fanlo
authors.forename: Blanca
authors.initials: B
authors.lastname: Sinués
authors.forename: Jesús F
authors.initials: JF
authors.lastname: Escanero
mesh_terms: D000328:Adult; D001642:Bicycling; D001786:Blood Glucose / Q000378:metabolism*; D003430:Cross-Sectional Studies; D005080:Exercise Test; D005230:Fatty Acids, Nonesterified / Q000097:blood*; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D019344:Lactic Acid / Q000097:blood*; D008297:Male; D053858:Metabolic Networks and Pathways / Q000235:genetics; D027501:Monocarboxylic Acid Transporters / Q000235:genetics*; D009154:Mutation; D010807:Physical Endurance / Q000502:physiology; D011110:Polymorphism, Genetic; D027981:Symporters / Q000235:genetics*; D055815:Young Adult; D000099233:Monocarboxylate Transport Protein 1
chemical_list: D001786:Blood Glucose; D005230:Fatty Acids, Nonesterified; D019344:Lactic Acid; D027501:Monocarboxylic Acid Transporters; D027981:Symporters; D000099233:Monocarboxylate Transport Protein 1