title: Molecular Characterisation and Diagnosis of Root-Knot Nematodes (Meloidogyne spp.) from Turfgrasses in North Carolina, USA.
abstract: Root-knot nematodes (Meloidogyne spp.) are the most common and destructive plant-parasitic nematode group worldwide and adversely influence both crop quality and yield. In this study, a total of 51 root-knot nematode populations from turfgrasses were tested, of which 44 were from North Carolina, 6 from South Carolina and 1 from Virginia. Molecular characterisation was performed on these samples by DNA sequencing on the ribosomal DNA 18S, ITS and 28S D2/D3. Species-specific primers were developed to identify turfgrass root-knot nematode through simplex or duplex PCR. Four species were identified, including M. marylandi Jepson & Golden in Jepson, 1987, M. graminis (Sledge & Golden, 1964) Whitehead, 1968, M. incognita (Kofoid & White, 1919) Chitwood, 1949 and M. naasi Franklin, 1965 through a combined analysis of DNA sequencing and PCR by species-specific primers. M. marylandi has been reported from North Carolina and South Carolina for the first time. Molecular diagnosis using PCR by species-specific primers provides a rapid and cheap species identification approach for turfgrass root-knot nematodes.
authors.affiliation: Nematode Assay Section, Agronomic Division, North Carolina Department of Agriculture & Consumer Services, Raleigh, North Carolina, United States of America.
authors.forename: Weimin
authors.initials: W
authors.lastname: Ye
authors.affiliation: Department of Plant Pathology, North Carolina State University, Raleigh, North Carolina, United States of America.|Department of Plant Protection, Zhongkai University of Agriculture and Engineering, Guangzhou, People's Republic of China.
authors.forename: Yongsan
authors.initials: Y
authors.lastname: Zeng
authors.affiliation: Department of Plant Pathology, North Carolina State University, Raleigh, North Carolina, United States of America.
authors.forename: James
authors.initials: J
authors.lastname: Kerns
mesh_terms: D000818:Animals; D017125:Genes, Protozoan; D058885:Multilocus Sequence Typing; D060885:Multiplex Polymerase Chain Reaction; D009657:North Carolina; D010802:Phylogeny; D010935:Plant Diseases / Q000469:parasitology*; D018517:Plant Roots / Q000469:parasitology*; D006109:Poaceae / Q000469:parasitology*; D014431:Tylenchoidea / Q000145:classification / Q000235:genetics*