title: Effect of recombinant interleukin-12 on murine skin regeneration and cell dynamics using in vivo multimodal microscopy.
abstract: Interleukin-12 (IL-12) is a pro-inflammatory cytokine known for its role in immunity, and previous studies have shown that IL-12 provides mitigation of radiation injury. In this study, we utilize a multimodal microscopy system equipped with second harmonic generation (SHG) and fluorescence lifetime imaging microscopy (FLIM) to examine the effect of IL-12 on collagen structure and cellular metabolic activity in vivo during skin wound healing. This preliminary study illustrates the highly dynamic and heterogeneous in vivo microenvironment of the wounded skin. In addition, results suggest that IL-12 triggers a significantly more rapid and greater cellular metabolic response in the wounded animals. These results can elucidate insights into the response mechanism of IL-12 in both wound healing and acute radiation syndrome.
authors.affiliation: Department of Bioengineering, University of Illinois at Urbana-Champaign, 1270 Digital Computer Laboratory, MC-278, 1304 W. Springfield Ave. Urbana, IL 61801, USA ; Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, 405 N. Mathews Ave. Urbana, IL 61801, USA.
authors.forename: Joanne
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, 306 N. Wright St. Urbana, IL 61801, USA ; Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, 405 N. Mathews Ave. Urbana, IL 61801, USA.
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Bower
authors.affiliation: Neumedicines Inc. 133 N. Altadena Dr. #310, Pasadena, CA 91107, USA.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Vainstein
authors.affiliation: Neumedicines Inc. 133 N. Altadena Dr. #310, Pasadena, CA 91107, USA.
authors.forename: Zoya
authors.initials: Z
authors.lastname: Gluzman-Poltorak
authors.affiliation: Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, 405 N. Mathews Ave. Urbana, IL 61801, USA.
authors.forename: Eric J
authors.initials: EJ
authors.lastname: Chaney
authors.affiliation: Department of Bioengineering, University of Illinois at Urbana-Champaign, 1270 Digital Computer Laboratory, MC-278, 1304 W. Springfield Ave. Urbana, IL 61801, USA ; Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, 405 N. Mathews Ave. Urbana, IL 61801, USA.
authors.forename: Marina
authors.initials: M
authors.lastname: Marjanovic
authors.affiliation: Neumedicines Inc. 133 N. Altadena Dr. #310, Pasadena, CA 91107, USA.
authors.forename: Lena A
authors.initials: LA
authors.lastname: Basile
authors.affiliation: Department of Bioengineering, University of Illinois at Urbana-Champaign, 1270 Digital Computer Laboratory, MC-278, 1304 W. Springfield Ave. Urbana, IL 61801, USA ; Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, 306 N. Wright St. Urbana, IL 61801, USA ; Department of Internal Medicine, University of Illinois at Urbana-Champaign, 190 Medical Science Building, MC-714, 506 S. Mathews Ave. Urbana, IL 61801, USA ; Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, 405 N. Mathews Ave. Urbana, IL 61801, USA.
authors.forename: Stephen A
authors.initials: SA
authors.lastname: Boppart
keywords: (170.2520) Fluorescence microscopy; (170.3880) Medical and biological imaging; (170.6920) Time-resolved imaging; (180.4315) Nonlinear microscopy; (190.1900) Diagnostic applications of nonlinear optics