title: Characterization of the iron-binding properties of pyoverdine using electron-capture dissociation-tandem mass spectrometry.
abstract: Pyoverdines (PVD) are a group of siderophores produced by fluorescent Pseudomonads. Identification of PVD variants mostly relies on liquid chromatography-tandem mass spectrometry (LC-MS/MS) using collision-induced dissociation (CID). Here, both CID and the novel dissociation technique electron-capture dissociation (ECD) were applied to characterize PVD succinamide and its Fe(III)-chelated complex. The results clearly showed that ECD produced diagnostic side chain fragmentation of the PVD peptide chain and preserved the labile Fe(III) binding to the chromophore in contrast to CID. The ECD technique is therefore expected to support the understanding of strain-specific Fe(III) transport processes of PVDs.
authors.affiliation: Institute of Bioanalytical Chemistry, Saarland University, 66123, Saarbrücken, Germany.
authors.forename: Yulin
authors.initials: Y
authors.lastname: Qi
authors.affiliation: Institute of Inorganic and Analytical Chemistry, University of Münster, Münster, Germany.
authors.forename: Heiko
authors.initials: H
authors.lastname: Hayen
authors.affiliation: Institute of Bioanalytical Chemistry, Saarland University, 66123, Saarbrücken, Germany. dietrich.volmer@mx.uni-saarland.de.
authors.forename: Dietrich A
authors.initials: DA
authors.lastname: Volmer
mesh_terms: D000577:Amides / Q000737:chemistry; D002853:Chromatography, Liquid; D004583:Electrons; D005290:Ferric Compounds / Q000737:chemistry; D007501:Iron / Q000737:chemistry*; D033862:Iron-Binding Proteins / Q000737:chemistry / Q000302:isolation & purification*; D009842:Oligopeptides / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification*; D017262:Siderophores / Q000737:chemistry; D013386:Succinates / Q000737:chemistry; D053719:Tandem Mass Spectrometry
chemical_list: D000577:Amides; D005290:Ferric Compounds; D033862:Iron-Binding Proteins; D009842:Oligopeptides; D010455:Peptides; D017262:Siderophores; D013386:Succinates; C042453:pyoverdin; D007501:Iron; C011116:succinamide
keywords: ECD; Electron-capture dissociation; FTICR-MS; Iron binding; Pyoverdine; Siderophores