title: [Pulse-modulated Electromagnetic Radiation of Extremely High Frequencies Protects Cellular DNA against Damaging Effect of Physico-Chemical Factors in vitro].
abstract: Using a comet assay technique, we investigated protective effects of. extremely high frequency electromagnetic radiation in combination with the damaging effect of X-ray irradiation, the effect of damaging agents hydrogen peroxide and methyl methanesulfonate on DNA in mouse whole blood leukocytes. It was shown that the preliminary exposure of the cells to low intensity pulse-modulated electromagnetic radiation (42.2 GHz, 0.1 mW/cm2, 20-min exposure, modulation frequencies of 1 and 16 Hz) caused protective effects decreasing the DNA damage by 20-45%. The efficacy of pulse-modulated electromagnetic radiation depended on the type of genotoxic agent and increased in a row methyl methanesulfonate--X-rays--hydrogen peroxide. Continuous electromagnetic radiation was ineffective. The mechanisms of protective effects may be connected with an induction of the adaptive response by nanomolar concentrations of reactive oxygen species formed by pulse-modulated electromagnetic radiation.
authors.forename: A B
authors.initials: AB
authors.lastname: Gapeyev
authors.forename: N A
authors.initials: NA
authors.lastname: Lukyanova
mesh_terms: D000818:Animals; D004247:DNA / Q000187:drug effects / Q000528:radiation effects*; D004249:DNA Damage / Q000187:drug effects / Q000235:genetics / Q000528:radiation effects*; D060733:Electromagnetic Radiation; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007962:Leukocytes / Q000187:drug effects / Q000528:radiation effects*; D051379:Mice; D014965:X-Rays
chemical_list: D004247:DNA; D006861:Hydrogen Peroxide