title: The biochemistry of blister fluid from pediatric burn injuries: proteomics and metabolomics aspects.
abstract: Burn injury is a prevalent and traumatic event for pediatric patients. At present, the diagnosis of burn injury severity is subjective and lacks a clinically relevant quantitative measure. This is due in part to a lack of knowledge surrounding the biochemistry of burn injuries and that of blister fluid. A more complete understanding of the blister fluid biochemistry may open new avenues for diagnostic and prognostic development. Burn insult induces a highly complex network of signaling processes and numerous changes within various biochemical systems, which can ultimately be examined using proteome and metabolome measurements. This review reports on the current understanding of burn wound biochemistry and outlines a technical approach for 'omics' profiling of blister fluid from burn wounds of differing severity.
authors.affiliation: a Tissue Repair and Regeneration Program , Institute of Health and Biomedical Innovation , Kelvin Grove , Australia.|b School of Biomedical Sciences , Queensland University of Technology , Brisbane , Australia.|c Wound Management Innovation Co-operative Research Centre , West End , Australia.
authors.forename: Tuo
authors.initials: T
authors.lastname: Zang
authors.affiliation: a Tissue Repair and Regeneration Program , Institute of Health and Biomedical Innovation , Kelvin Grove , Australia.|b School of Biomedical Sciences , Queensland University of Technology , Brisbane , Australia.|c Wound Management Innovation Co-operative Research Centre , West End , Australia.
authors.forename: Daniel A
authors.initials: DA
authors.lastname: Broszczak
authors.affiliation: a Tissue Repair and Regeneration Program , Institute of Health and Biomedical Innovation , Kelvin Grove , Australia.|b School of Biomedical Sciences , Queensland University of Technology , Brisbane , Australia.|c Wound Management Innovation Co-operative Research Centre , West End , Australia.
authors.forename: James A
authors.initials: JA
authors.lastname: Broadbent
authors.affiliation: a Tissue Repair and Regeneration Program , Institute of Health and Biomedical Innovation , Kelvin Grove , Australia.|b School of Biomedical Sciences , Queensland University of Technology , Brisbane , Australia.|d Centre for Children's Burns and Trauma Research , Queensland University of Technology, Institute of Health and Biomedical Innovation at the Centre for Children's Health Research , South Brisbane , Australia.
authors.forename: Leila
authors.initials: L
authors.lastname: Cuttle
authors.affiliation: a Tissue Repair and Regeneration Program , Institute of Health and Biomedical Innovation , Kelvin Grove , Australia.|b School of Biomedical Sciences , Queensland University of Technology , Brisbane , Australia.
authors.forename: Haitao
authors.initials: H
authors.lastname: Lu
authors.affiliation: a Tissue Repair and Regeneration Program , Institute of Health and Biomedical Innovation , Kelvin Grove , Australia.|b School of Biomedical Sciences , Queensland University of Technology , Brisbane , Australia.
authors.forename: Tony J
authors.initials: TJ
authors.lastname: Parker
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D001768:Blister / Q000378:metabolism*; D002056:Burns / Q000378:metabolism*; D002648:Child; D045604:Extracellular Fluid / Q000378:metabolism; D006801:Humans; D055442:Metabolome; D055432:Metabolomics; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics; D014945:Wound Healing
chemical_list: D015415:Biomarkers; D020543:Proteome
keywords: blister fluid; mass spectrometry; metabolomics; nuclear magnetic resonance; pediatric burns; proteomics