title: [State of Fungal Lipases of Rhizopus microsporus, Penicillium sp. and Oospora lactis in Border Layers Water-Solid Phase and Factors Affecting Catalytic Properties of Enzymes].
abstract: We demonstrated that a change in the catalytic activity of fungal lipases synthesized by Rhizopus microsporus, Penicillium sp. and Oospora lactis and their ability to absorb on different sorbents depended on the nature of groups on the solid phase surface in the model systems water: lipid and water: solid phase. Thus, the stability of Penicillium sp. lipases increased 85% in the presence ofsorsilen or DEAE-cellulose, and 55% of their initial activity respectively was preserved. In the presence of silica gel and CM-cellulose, a decreased rate of lipid hydrolysis by Pseudomonas sp. enzymes was observed in water medium, and the hydrolysis rate increased by 2.4 and 1.5 times respectively in the presence of aminoaerosil and polykefamid. In an aqueous-alcohol medium, aminoaerosil and polykefamid decreased the rate of substrate hydrolysis by more than 30 times. The addition of aerosil to aqueous and aqueous-alcohol media resulted in an increase in the hydrolysis rate by 1.2-1.3 times. Sorsilen stabilized Penicillium sp. lipase activity at 40, 45, 50 and 55 degrees C. Either stabilization or inactivation of lipases was observed depending on the pH of the medium and the nature of chemical groups localized on the surface of solid phase. The synthetizing activity of lipases also changed depending on the conditions.
authors.forename: Kh T
authors.initials: KhT
authors.lastname: Khasanov
authors.forename: K
authors.initials: K
authors.lastname: Davranov
authors.forename: M M
authors.initials: MM
authors.lastname: Rakhimov
mesh_terms: D002384:Catalysis; D006868:Hydrolysis; D008049:Lipase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008055:Lipids / Q000737:chemistry; D010407:Penicillium / Q000201:enzymology* / Q000235:genetics; D044367:Phase Transition; D012233:Rhizopus / Q000201:enzymology* / Q000235:genetics; D013379:Substrate Specificity; D014867:Water / Q000737:chemistry / Q000378:metabolism
chemical_list: D008055:Lipids; D014867:Water; D008049:Lipase