title: Metabolic Interplay between the Asian Citrus Psyllid and Its Profftella Symbiont: An Achilles' Heel of the Citrus Greening Insect Vector.
abstract: 'Candidatus Liberibacter asiaticus' (CLas), the bacterial pathogen associated with citrus greening disease, is transmitted by Diaphorina citri, the Asian citrus psyllid. Interactions among D. citri and its microbial endosymbionts, including 'Candidatus Profftella armatura', are likely to impact transmission of CLas. We used quantitative mass spectrometry to compare the proteomes of CLas(+) and CLas(-) populations of D. citri, and found that proteins involved in polyketide biosynthesis by the endosymbiont Profftella were up-regulated in CLas(+) insects. Mass spectrometry analysis of the Profftella polyketide diaphorin in D. citri metabolite extracts revealed the presence of a novel diaphorin-related polyketide and the ratio of these two polyketides was changed in CLas(+) insects. Insect proteins differentially expressed between CLas(+) and CLas(-) D. citri included defense and immunity proteins, proteins involved in energy storage and utilization, and proteins involved in endocytosis, cellular adhesion, and cytoskeletal remodeling which are associated with microbial invasion of host cells. Insight into the metabolic interdependence between the insect vector, its endosymbionts, and the citrus greening pathogen reveals novel opportunities for control of this disease, which is currently having a devastating impact on citrus production worldwide.
authors.affiliation: Boyce Thompson Institute for Plant Research, Ithaca, New York, United States of America.|Robert W. Holley Center for Agriculture and Health, Emerging Pests and Pathogens Research Unit, USDA Agricultural Research Service, Ithaca, New York, United States of America.
authors.forename: John S
authors.initials: JS
authors.lastname: Ramsey
authors.affiliation: Department of Genome Sciences, University of Washington, Seattle, Washington, United States of America.
authors.forename: Richard S
authors.initials: RS
authors.lastname: Johnson
authors.affiliation: Boyce Thompson Institute for Plant Research, Ithaca, New York, United States of America.|Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York, United States of America.
authors.forename: Jason S
authors.initials: JS
authors.lastname: Hoki
authors.affiliation: Boyce Thompson Institute for Plant Research, Ithaca, New York, United States of America.|Plant Pathology and Plant-Microbe Biology Section, School of Integrative Plant Science, Cornell University, Ithaca, New York, United States of America.
authors.forename: Angela
authors.initials: A
authors.lastname: Kruse
authors.affiliation: Boyce Thompson Institute for Plant Research, Ithaca, New York, United States of America.|Robert W. Holley Center for Agriculture and Health, Emerging Pests and Pathogens Research Unit, USDA Agricultural Research Service, Ithaca, New York, United States of America.
authors.forename: Jaclyn
authors.initials: J
authors.lastname: Mahoney
authors.affiliation: U.S. Horticultural Research Laboratory, Subtropical Plant Pathology Research Unit, USDA Agricultural Research Service, Ft. Pierce, Florida, United States of America.
authors.forename: Mark E
authors.initials: ME
authors.lastname: Hilf
authors.affiliation: U.S. Horticultural Research Laboratory, Subtropical Insects and Horticulture Research Unit, USDA Agricultural Research Service, Ft. Pierce, Florida, United States of America.
authors.forename: Wayne B
authors.initials: WB
authors.lastname: Hunter
authors.affiliation: U.S. Horticultural Research Laboratory, Subtropical Insects and Horticulture Research Unit, USDA Agricultural Research Service, Ft. Pierce, Florida, United States of America.
authors.forename: David G
authors.initials: DG
authors.lastname: Hall
authors.affiliation: Boyce Thompson Institute for Plant Research, Ithaca, New York, United States of America.|Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York, United States of America.
authors.forename: Frank C
authors.initials: FC
authors.lastname: Schroeder
authors.affiliation: Department of Genome Sciences, University of Washington, Seattle, Washington, United States of America.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: MacCoss
authors.affiliation: Boyce Thompson Institute for Plant Research, Ithaca, New York, United States of America.|Robert W. Holley Center for Agriculture and Health, Emerging Pests and Pathogens Research Unit, USDA Agricultural Research Service, Ithaca, New York, United States of America.|Plant Pathology and Plant-Microbe Biology Section, School of Integrative Plant Science, Cornell University, Ithaca, New York, United States of America.
authors.forename: Michelle
authors.initials: M
authors.lastname: Cilia
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D002957:Citrus / Q000382:microbiology*; D005786:Gene Expression Regulation; D006430:Hemiptera / Q000235:genetics / Q000276:immunology / Q000382:microbiology*; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D053858:Metabolic Networks and Pathways; D058977:Molecular Sequence Annotation; D010935:Plant Diseases / Q000382:microbiology; D061065:Polyketides / Q000378:metabolism*; D020543:Proteome / Q000235:genetics* / Q000378:metabolism; D012230:Rhizobiaceae / Q000502:physiology; D013559:Symbiosis
chemical_list: D001426:Bacterial Proteins; D019476:Insect Proteins; D061065:Polyketides; D020543:Proteome