title: Promoting endothelialization on decellularized porcine aortic valve by immobilizing branched polyethylene glycolmodified with cyclic-RGD peptide: an in vitro study.
abstract: We functionally modify a decellularized porcine aortic valve using a novel complex biologically active cyclic- (c)-RGD modified with branched polyethylene glycol (PEG), namely, c-RDG-PEG. Human umbilical vein endothelial cell (HUVEC) adhesion and proliferation were detected for up to 8 d after seeding on the scaffold. (1)H nuclear magnetic resonance (D2O) showed signal peaks at 7.27 and 7.38 ppm associated with protons of the phenyl group in c-RGD-PEG. Attenuated total reflectance Fourier transform infrared spectroscopy showed characteristic peaks for PEG at 1100 and 1342 cm(-1). These represented vibration peaks of C-O and -CH2 bonds, suggesting successful grafting of c-RGD-PEG to a decellularized porcine aortic valve (DPAV). The tensile strengths were significantly increased in the c-RGD-PEG-DPAV group compared to the native valve and DPAV groups (P < 0.05), while the elastic modulus was sigficantly decreased in the c-RGD-PEG-DPAV group compared to the native valve and DPAV groups (P < 0.05). HUVEC proliferation was significantly higher in the c-RGD-PEG-DPAV group than in the PEG-DPAV and DPAV groups (P < 0.01). Maximum adhesion occurred at 4 h, and on the 8th day, a confluent and compact monolayer formed on the valve surface. The modified DPAV resulted in good adhesion and proliferation of endothelial cells and is an appropriate approach to modify tissue engineered heart valves for promoting endothelialization.
authors.affiliation: Department of Cardiothoracic Surgery, The Second Affiliated Hospital of Nanchang University, Nanchang 330006, People's Republic of China. These authors contributed equally to this study and share the first authorship.
authors.forename: Jianliang
authors.initials: J
authors.lastname: Zhou
authors.forename: Bin'en
authors.initials: B
authors.lastname: Nie
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Zhu
authors.forename: Jingli
authors.initials: J
authors.lastname: Ding
authors.forename: Wei
authors.initials: W
authors.lastname: Yang
authors.forename: Jiawei
authors.initials: J
authors.lastname: Shi
authors.forename: Xiao
authors.initials: X
authors.lastname: Dong
authors.forename: Jianjun
authors.initials: J
authors.lastname: Xu
authors.forename: Nianguo
authors.initials: N
authors.lastname: Dong
mesh_terms: D000327:Adsorption; D000818:Animals; D001021:Aortic Valve / Q000737:chemistry*; D001705:Bioprosthesis; D002474:Cell-Free System; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000008:administration & dosage / Q000138:chemical synthesis; D042783:Endothelial Cells / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006350:Heart Valve Prosthesis; D006801:Humans; D018919:Neovascularization, Physiologic / Q000187:drug effects / Q000502:physiology*; D009842:Oligopeptides / Q000008:administration & dosage* / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry*; D013552:Swine; D054457:Tissue Scaffolds
chemical_list: D020099:Coated Materials, Biocompatible; D009842:Oligopeptides; D011092:Polyethylene Glycols; C047981:arginyl-glycyl-aspartic acid