title: Hollow core photonic crystal fiber for monitoring leukemia cells using surface enhanced Raman scattering (SERS).
abstract: The present paper demonstrates an antibody-free, robust, fast, and portable platform for detection of leukemia cells using Raman spectroscopy with a 785-nm laser diode coupled to a hollow core photonic crystal (HC-PCF) containing silver nanoparticles. Acute myeloid leukemia is one of the most common bone marrow cancers in children and youths. Clinical studies suggest that early diagnosis and remission evaluation of myoblasts in the bone marrow are pivotal for improving patient survival. However, the current protocols for leukemic cells detection involve the use of expensive antibodies and flow cytometers. Thus, we have developed a new technology for detection of leukemia cells up to 300 cells/ml using a compact fiber HC-PCF, which offers a novel alternative to existing clinical standards. Furthermore, we were also able to accurately distinguish live, apoptotic and necrotic leukemic cells.
authors.affiliation: School of Electrical Engineering and Computer Science (EECS), University of Ottawa, Ottawa, ON K1N 6N5, Canada.
authors.forename: Altaf
authors.initials: A
authors.lastname: Khetani
authors.affiliation: School of Electrical Engineering and Computer Science (EECS), University of Ottawa, Ottawa, ON K1N 6N5, Canada.
authors.forename: Ali
authors.initials: A
authors.lastname: Momenpour
authors.affiliation: University of Ottawa Heart Institute, Division of Cardiac Surgery Research, 40 Ruskin Street, Ottawa, ON K1Y 4W7 Canada.
authors.forename: Emilio I
authors.initials: EI
authors.lastname: Alarcon
authors.affiliation: School of Electrical Engineering and Computer Science (EECS), University of Ottawa, Ottawa, ON K1N 6N5, Canada.
authors.forename: Hanan
authors.initials: H
authors.lastname: Anis
keywords: (060.5295) Photonic crystal fibers; (170.1530) Cell analysis; (170.5660) Raman spectroscopy; (240.6695) Surface-enhanced Raman scattering; (280.4788) Optical sensing and sensors