title: Characterization of a gamma-butyrolactone synthetase gene homologue (stcA) involved in bafilomycin production and aerial mycelium formation in Streptomyces sp. SBI034.
abstract: Streptomyces SBI034 produces several bafilomycin derivatives. Its afsA homologue (stcA) and putative γ-butyrolactone receptor gene (stcB) were cloned. Construction of a stcA disruptant (stcA gene knockout) resulted in complete abolishment of all bafilomycin production. Electron microscopic analysis showed a defect of aerial mycelium formation and sporulation in the stcA disruptant. Restoration of all phenotypic defects and bafilomycin production was observed in a stcA complemented strain. Addition of exogenous γ-butyrolactone (GBL) extracted from the culture broth of the wild-type strain could stimulate the aerial mycelium and spore formation of the stcA disruptant. These results suggest that stcA plays a role in GBL-mediated regulation of bafilomycin biosynthesis and morphological development in Streptomyces strain SBI034.
authors.affiliation: Department of Biotechnology, Faculty of Science, Mahidol University, Bangkok, 10400, Thailand.|Osaka Collaborative Research Center on Bioscience and Biotechnology, Faculty of Science, Mahidol University, Bangkok, 10400, Thailand.
authors.forename: Bungonsiri
authors.initials: B
authors.lastname: Intra
authors.affiliation: Department of Biotechnology, Faculty of Science, Mahidol University, Bangkok, 10400, Thailand.|Osaka Collaborative Research Center on Bioscience and Biotechnology, Faculty of Science, Mahidol University, Bangkok, 10400, Thailand.
authors.forename: Jirayut
authors.initials: J
authors.lastname: Euanorasetr
authors.affiliation: Osaka Collaborative Research Center on Bioscience and Biotechnology, Faculty of Science, Mahidol University, Bangkok, 10400, Thailand.|International Center for Biotechnology, Osaka University, Osaka, 565-0871, Japan.
authors.forename: Takuya
authors.initials: T
authors.lastname: Nihira
authors.affiliation: Department of Biotechnology, Faculty of Science, Mahidol University, Bangkok, 10400, Thailand. watanalai.pan@mahidol.ac.th.|Osaka Collaborative Research Center on Bioscience and Biotechnology, Faculty of Science, Mahidol University, Bangkok, 10400, Thailand. watanalai.pan@mahidol.ac.th.
authors.forename: Watanalai
authors.identifier: http://orcid.org/0000-0003-0141-9279
authors.initials: W
authors.lastname: Panbangred
mesh_terms: D015107:4-Butyrolactone / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000378:metabolism*; D055786:Gene Knockout Techniques; D005816:Genetic Complementation Test; D008025:Ligases / Q000235:genetics* / Q000378:metabolism*; D018942:Macrolides / Q000378:metabolism*; D013171:Spores, Bacterial / Q000254:growth & development; D013302:Streptomyces / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism*
chemical_list: D004791:Enzyme Inhibitors; D018942:Macrolides; C057620:bafilomycin A; D008025:Ligases; D015107:4-Butyrolactone
keywords: A-factor; Bafilomycin; Streptomyces; γ-Butyrolactone regulatory system