title: Chemical composition and antibiofilm activity of Petroselinum crispum and Ocimum basilicum essential oils against Vibrio spp. strains.
abstract: In this study, we evaluated the antibacterial activity of parsley and basilic essential oils tested against Vibrio strains and their abilities to inhibit and eradicate the mature biofilm using the XTT assay. Petroselinum crispum essential oil was characterized by 1,3,8-p-menthatriene (24.2%), β-phellandrene (22.8%), apiol (13.2%), myristicin (12.6%) and terpinolene (10.3%) as a major constituents. While, in the basilic oil, linalool (42.1%), (E)-methylcinnamate (16.9%) and 1-8 cineole (7.6%) were the main ones. These two essential oils exhibit high anti-Vibrio spp. activity with varying magnitudes. All microorganisms were strongly affected indicating an appreciable antimicrobial potential of basilic with a diameter of inhibition zones growth ranging from 8.67 to 23.33 mm and MIC and MBC values ranging from (0.023-0.047 mg/ml) and (>3->24 mg/ml), respectively. The two essential oils can inhibit and eradicate the mature biofilm formed on polystyrene surface even at low concentrations, with high magnitude for Ocimum basilicum essential oil. This study gives a better insight into the anti-Vibrio activity of parsley and basilc oils and the possibility of their use to prevent and eradicate contamination of sea products by these strains.
authors.affiliation: Water Research and Technologies Center (CERTE), Laboratory of Wastewater Treatment, P.O. Box 273, 8020, Soliman, Tunis, Tunisia. Electronic address: snmejdi@yahoo.fr.
authors.forename: Mejdi
authors.initials: M
authors.lastname: Snoussi
authors.affiliation: Water Research and Technologies Center (CERTE), Laboratory of Wastewater Treatment, P.O. Box 273, 8020, Soliman, Tunis, Tunisia.
authors.forename: Ameni
authors.initials: A
authors.lastname: Dehmani
authors.affiliation: Laboratory of Infectious Diseases and Biological Agents, Faculty of Pharmacy, University of Monastir, TU-5000, Monastir, Tunisia.
authors.forename: Emira
authors.initials: E
authors.lastname: Noumi
authors.affiliation: Department of Pharmacy, University of Pisa, Via Bonanno 6, 56126, Pisa, Italy.
authors.forename: Guido
authors.initials: G
authors.lastname: Flamini
authors.affiliation: Department of Drug Sciences, University of Pavia, Viale Taramelli 12, 27100, Pavia, Italy.
authors.forename: Adele
authors.initials: A
authors.lastname: Papetti
mesh_terms: D016980:Aeromonas hydrophila / Q000187:drug effects; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000032:analysis / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects*; D008401:Gas Chromatography-Mass Spectrometry; D008826:Microbial Sensitivity Tests; D031339:Ocimum; D018649:Ocimum basilicum / Q000737:chemistry*; D009822:Oils, Volatile / Q000494:pharmacology; D028528:Petroselinum / Q000737:chemistry*; D010938:Plant Oils / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D017747:Seafood / Q000382:microbiology; D012623:Seawater / Q000382:microbiology; D014733:Vibrio / Q000187:drug effects* / Q000502:physiology
chemical_list: D000900:Anti-Bacterial Agents; D009822:Oils, Volatile; D010938:Plant Oils; C080118:ocimum oil; C051168:basil oil
keywords: Anti-Vibrio spp.; Biofilm; Chemical composition; Ocimum basilicum; Petroselinum crispum