title: Large area magnetic micropallet arrays for cell colony sorting.
abstract: A new micropallet array platform for adherent cell colony sorting has been developed. The platform consisted of thousands of square plastic pallets, 270 μm by 270 μm on each side, large enough to hold a single colony of cells. Each pallet included a magnetic core, allowing them to be collected with a magnet after being released using a microscope mounted laser system. The micropallets were patterned from 1002F epoxy resist and were fabricated on translucent, gold coated microscope slides. The gold layer was used as seed for electroplating the ferromagnetic cores within every individual pallet. The gold layer also facilitated the release of each micropallet during laser release. This array allows for individual observation, sorting and collection of isolated cell colonies for biological cell colony research. In addition to consistent release and recovery of individual colonies, we demonstrated stable biocompatibility and minimal loss in imaging quality compared to previously developed micropallet arrays.
authors.affiliation: Biomedical Engineering, University of California, Irvine, CA, USA. mbachman@uci.edu.
authors.forename: Wesley A
authors.initials: WA
authors.lastname: Cox-Muranami
authors.affiliation: School of Medicine, Department of Medicine, School of Biological Sciences, Department of Molecular Biology and Biochemistry, University of California, Irvine, CA, USA.
authors.forename: Edward L
authors.initials: EL
authors.lastname: Nelson
authors.affiliation: Biomedical Engineering, University of California, Irvine, CA, USA. mbachman@uci.edu.
authors.forename: G P
authors.initials: GP
authors.lastname: Li
authors.affiliation: Biomedical Engineering, University of California, Irvine, CA, USA. mbachman@uci.edu.
authors.forename: Mark
authors.initials: M
authors.lastname: Bachman
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000295:instrumentation*; D002460:Cell Line; D002469:Cell Separation / Q000295:instrumentation*; D002470:Cell Survival; D006046:Gold / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D007834:Lasers; D060328:Magnetic Phenomena; D051379:Mice; D041681:NIH 3T3 Cells; D009532:Nickel / Q000737:chemistry; D051381:Rats; D046888:Tissue Array Analysis / Q000295:instrumentation*
chemical_list: D006046:Gold; D009532:Nickel