title: Exopolysaccharide Produced by Lactobacillus Plantarum Induces Maturation of Dendritic Cells in BALB/c Mice.
abstract: Lactobacillus plantarum (L. plantarum) exopolysaccharide (EPS) is an important bioactive component in fermented functional foods. However, there is a lack of data concerning the effects of L. plantarum EPS on maturation of mouse dendritic cells (DCs). In this study, we purified L. plantarum EPS and examined its effects on cytokines production by dendritic cells in serum and intestinal fluid of BALB/c mice, then investigated its effects on phenotypic and functional maturation of mouse bone marrow-derived dendritic cells (BMDCs). Cytokines (nitric oxide, IL-12p70, IL-10 and RANTES) in serum and intestinal fluid were analyzed by enzyme linked immunosorbent assay (ELISA) after the mice received EPS for 2, 5 and 7 days, respectively. DCs derived from bone marrow of BALB/c mouse were treated with EPS, then the phenotypic maturation of BMDCs was analyzed using flow cytometer and the functional maturation of BMDCs was analyzed by ELISA, and, lastly, mixed lymphocyte proliferation was performed. We found the molecular weight of purified EPS was approximately 2.4×106 Da and it was composed of ribose, rhamnose, arabinose, xylose, mannose, glucose and galactose in a molar ratio of 2:1:1:10:4:205:215. We observed that L. plantarum EPS enriched production of nitric oxide, IL-12p70 and RANTES, and decreased the secretion of IL-10 in the serum or intestinal fluid as well as in the supernatant of DCs treated with the EPS. The EPS also up-regulated the expression of MHC II and CD86 on DCs surface and promoted T cells to proliferate in vitro. Our data provide direct evidence to suggest that L. plantarum EPS can effectively induce maturation of DCs in mice.
authors.affiliation: Key Laboratory of Dairy Science, Ministry of Education; College of Food Science, Northeast Agricultural University, Harbin, China.|Synergetic Innovation Center of Food Safety and Nutrition, Harbin, China.|College of Food Science, Heilongjiang Bayi Agricultural University, Daqing, China.
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Tang
authors.affiliation: Key Laboratory of Dairy Science, Ministry of Education; College of Food Science, Northeast Agricultural University, Harbin, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Dong
authors.affiliation: Key Laboratory of Dairy Science, Ministry of Education; College of Food Science, Northeast Agricultural University, Harbin, China.
authors.forename: Keyu
authors.initials: K
authors.lastname: Wan
authors.affiliation: Key Laboratory of Dairy Science, Ministry of Education; College of Food Science, Northeast Agricultural University, Harbin, China.|Synergetic Innovation Center of Food Safety and Nutrition, Harbin, China.
authors.forename: Ligang
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Dairy Science, Ministry of Education; College of Food Science, Northeast Agricultural University, Harbin, China.|Synergetic Innovation Center of Food Safety and Nutrition, Harbin, China.
authors.forename: Chun
authors.initials: C
authors.lastname: Li
authors.affiliation: Key Laboratory of Dairy Science, Ministry of Education; College of Food Science, Northeast Agricultural University, Harbin, China.|Synergetic Innovation Center of Food Safety and Nutrition, Harbin, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Dairy Science, Ministry of Education; College of Food Science, Northeast Agricultural University, Harbin, China.|National Dairy Engineering & Research Center, Harbin, China.|Synergetic Innovation Center of Food Safety and Nutrition, Harbin, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Liu
mesh_terms: D000818:Animals; D001089:Arabinose / Q000378:metabolism; D003713:Dendritic Cells / Q000166:cytology* / Q000187:drug effects*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005434:Flow Cytometry; D005690:Galactose / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D048191:Lactiplantibacillus plantarum / Q000378:metabolism*; D008358:Mannose / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D011134:Polysaccharides / Q000378:metabolism* / Q000494:pharmacology*; D012210:Rhamnose / Q000378:metabolism; D012266:Ribose / Q000378:metabolism; D014994:Xylose / Q000378:metabolism
chemical_list: D011134:Polysaccharides; D012266:Ribose; D014994:Xylose; D001089:Arabinose; D005947:Glucose; D008358:Mannose; D012210:Rhamnose; D005690:Galactose