title: LEF-1 Regulates Tyrosinase Gene Transcription In Vitro.
abstract: TYR, DCT and MITF are three important genes involved in maintaining the mature phenotype and producing melanin; they therefore participate in neural crest cell development into melanocytes. Previous studies have revealed that the Wnt signaling factor lymphoid enhancer-binding factor (LEF-1) can enhance DCT and MITF gene expression. However, whether LEF-1 also affects TYR gene expression remains unclear. In the present study, we found that LEF-1 regulated TYR transcription in vitro. LEF-1 overexpression increased TYR gene promoter activity, whereas LEF-1 knockdown by RNA interference significantly decreased TYR expression. Moreover, the core GTTTGAT sequence (-56 to -50) within the TYR promoter is essential for the effect of LEF-1 on TYR expression, and chromatin immunoprecipitation (ChIP) assay indicated that endogenous LEF-1 interacts with the TYR promoter. In addition, we observed a synergistic transactivation of the TYR promoter by LEF-1 and MITF. These data suggest that Wnt signaling plays an important role in regulating melanocyte development and differentiation.
authors.affiliation: Department of Otolaryngology Head and Neck Surgery, Xiangya Hospital, Central South University, Changsha, Hunan, People's Republic of China.
authors.forename: Xueping
authors.initials: X
authors.lastname: Wang
authors.affiliation: Province Key Laboratory of Otolaryngology Critical Disease, Xiangya Hospital, Central South University, Changsha, Hunan, People's Republic of China.
authors.forename: Yalan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Otolaryngology Head and Neck Surgery, Xiangya Hospital, Central South University, Changsha, Hunan, People's Republic of China.|Province Key Laboratory of Otolaryngology Critical Disease, Xiangya Hospital, Central South University, Changsha, Hunan, People's Republic of China.
authors.forename: Hongsheng
authors.initials: H
authors.lastname: Chen
authors.affiliation: Department of Otolaryngology Head and Neck Surgery, Xiangya Hospital, Central South University, Changsha, Hunan, People's Republic of China.|Province Key Laboratory of Otolaryngology Critical Disease, Xiangya Hospital, Central South University, Changsha, Hunan, People's Republic of China.
authors.forename: Lingyun
authors.initials: L
authors.lastname: Mei
authors.affiliation: Department of Otolaryngology Head and Neck Surgery, Xiangya Hospital, Central South University, Changsha, Hunan, People's Republic of China.|Province Key Laboratory of Otolaryngology Critical Disease, Xiangya Hospital, Central South University, Changsha, Hunan, People's Republic of China.
authors.forename: Chufeng
authors.initials: C
authors.lastname: He
authors.affiliation: Department of Otolaryngology Head and Neck Surgery, Xiangya Hospital, Central South University, Changsha, Hunan, People's Republic of China.|Province Key Laboratory of Otolaryngology Critical Disease, Xiangya Hospital, Central South University, Changsha, Hunan, People's Republic of China.
authors.forename: Lu
authors.initials: L
authors.lastname: Jiang
authors.affiliation: Department of Otolaryngology Head and Neck Surgery, Xiangya Hospital, Central South University, Changsha, Hunan, People's Republic of China.
authors.forename: Zhijie
authors.initials: Z
authors.lastname: Niu
authors.affiliation: Department of Otolaryngology Head and Neck Surgery, Xiangya Hospital, Central South University, Changsha, Hunan, People's Republic of China.|Department of Otolaryngology, 1st Affiliated Hospital, Xinjiang Medical University, Urumqi, People's Republic of China.
authors.forename: Jie
authors.initials: J
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Medical Genetics, Central South University, Changsha, Hunan, People's Republic of China.
authors.forename: Hunjin
authors.initials: H
authors.lastname: Luo
authors.affiliation: State Key Laboratory of Medical Genetics, Central South University, Changsha, Hunan, People's Republic of China.
authors.forename: Jiada
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Otolaryngology Head and Neck Surgery, Xiangya Hospital, Central South University, Changsha, Hunan, People's Republic of China.|Province Key Laboratory of Otolaryngology Critical Disease, Xiangya Hospital, Central South University, Changsha, Hunan, People's Republic of China.|State Key Laboratory of Medical Genetics, Central South University, Changsha, Hunan, People's Republic of China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Feng
mesh_terms: D000818:Animals; D001665:Binding Sites; D002454:Cell Differentiation; D002843:Chromatin / Q000737:chemistry / Q000378:metabolism; D047369:Chromatin Immunoprecipitation; D005786:Gene Expression Regulation; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D019746:Intramolecular Oxidoreductases / Q000235:genetics / Q000378:metabolism; D051821:Lymphoid Enhancer-Binding Factor 1 / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D008544:Melanocytes / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D051739:Microphthalmia-Associated Transcription Factor / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D014442:Monophenol Monooxygenase / Q000235:genetics* / Q000378:metabolism; D041681:NIH 3T3 Cells; D059372:Nucleotide Motifs; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic; D060449:Wnt Signaling Pathway
chemical_list: D002843:Chromatin; C497573:LEF1 protein, human; D051821:Lymphoid Enhancer-Binding Factor 1; C497234:MITF protein, human; D051739:Microphthalmia-Associated Transcription Factor; D034741:RNA, Small Interfering; D014442:Monophenol Monooxygenase; D019746:Intramolecular Oxidoreductases; C042501:dopachrome isomerase