title: TCP2 positively regulates HY5/HYH and photomorphogenesis in Arabidopsis.
abstract: Light regulates plant growth and development via multiple photoreceptors including phytochromes and cryptochromes. Although the functions of photoreceptors have been studied extensively, questions remain regarding the involvement of cryptochromes in photomorphogenesis. In this study, we identified a protein, TEOSINTE-LIKE1, CYCLOIDEA, and PROLIFERATING CELL FACTOR 2 (TCP2), which interacts with the cryptochrome 1 (CRY1) protein in yeast and plant cells via the N-terminal domains of both proteins. Transgenic plants overexpressing TCP2 displayed a light-dependent short hypocotyl phenotype, especially in response to blue light. Moreover, light affected TCP2 expression in a wavelength-dependent manner and TCP2 positively regulates mRNA expression of HYH and HY5. These results support the hypothesis that TCP2 is a transcription activator which acts downstream of multiple photoreceptors, including CRY1.
authors.affiliation: College of Biology, Hunan University, Changsha 410082, China.
authors.forename: Zhimin
authors.initials: Z
authors.lastname: He
authors.affiliation: College of Biology, Hunan University, Changsha 410082, China.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Zhao
authors.affiliation: College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China.
authors.forename: Fanna
authors.initials: F
authors.lastname: Kong
authors.affiliation: College of Plant Sciences, Jilin University, Changchun 130062, Jilin, China xml05@hnu.edu.cn zuozengcheng7@163.com.
authors.forename: Zecheng
authors.initials: Z
authors.lastname: Zuo
authors.affiliation: College of Biology, Hunan University, Changsha 410082, China State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082, China xml05@hnu.edu.cn zuozengcheng7@163.com.
authors.forename: Xuanming
authors.initials: X
authors.lastname: Liu
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development* / Q000528:radiation effects*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D050976:Basic-Leucine Zipper Transcription Factors / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D056931:Cryptochromes / Q000378:metabolism; D004268:DNA-Binding Proteins; D018506:Gene Expression Regulation, Plant / Q000528:radiation effects; D008027:Light; D009024:Morphogenesis / Q000528:radiation effects*; D009687:Nuclear Proteins / Q000378:metabolism*; D010641:Phenotype; D059828:Plant Cells / Q000378:metabolism / Q000528:radiation effects; D030821:Plants, Genetically Modified; D011485:Protein Binding / Q000528:radiation effects; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D015398:Signal Transduction / Q000528:radiation effects; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D050976:Basic-Leucine Zipper Transcription Factors; C538962:CRY1 protein, Arabidopsis; D002352:Carrier Proteins; D056931:Cryptochromes; D004268:DNA-Binding Proteins; C109254:HY5 protein, Arabidopsis; C457459:HYH protein, Arabidopsis; D009687:Nuclear Proteins; C000611766:TCP2 protein, Arabidopsis; D014157:Transcription Factors
keywords: Arabidopsis; CRY1; ChIP-qPCR; HY5; HYH; TCP2.; blue light signal transduction; hypocotyls