title: Extracellular Signal-Regulated Kinase 5 in the Cerebrospinal Fluid-Contacting Nucleus Contributes to Neuropathic Pain in Rats.
abstract: BACKGROUND
  The activation of mitogen-activated protein kinases (MAPKs) have been observed in synaptic plasticity processes of learning and memory in neuropathic pain. Cerebrospinal fluid-contacting nucleus (CSF-CN) has been identified with the onset and persistence of neuropathic pain. However, whether extracellular signal-regulated protein kinase 5 (ERK5), a member of MAPKs, in CSF-CN participates in neuropathic pain has not been studied yet.
  
  
  OBJECTIVE
  The aim of the present study was to identify the role of ERK5 in CSF-CN on the formation and development of neuropathic pain, and to investigate its possible mechanism.
  
  
  METHODS
  Controlled animal study.
  
  
  METHODS
  University laboratory.
  
  
  METHODS
  After a chronic constriction injury (CCI) model was produced, BIX02188 was dissolved in 1% DMSO and injected into the lateral ventricles LV in a volume of 3 μl with different doses (0.1 μg, 1 μg, 10 μg). Mechanical allodynia and thermal hypersensitivity behavioral test, immunofluorescence, and western blot technique were used in this research.
  
  
  RESULTS
  Following CCI, mechanical allodynia and thermal hypersensitivity were developed within a day, peaked at 14 days, and persisted for 21 days. ERK5 was remarkably activated by CCI in CSF-CN. Moreover, selective inhibiting of p-ERK5 expression in CSF-CN by BIX02188 could significantly relieve CCI-induced mechanical allodynia and thermal hypersensitivity, accompanying with the decreased phosphorylation of cAMP response-element binding protein (CREB) in CSF-CN.
  
  
  CONCLUSIONS
  More underlying mechanism(s) of the role of ERK5 in CSF-CN on the formation and development of neuropathic pain will be needed to explore in future research.
  
  
  CONCLUSIONS
  These findings suggest activation of ERK5 in CSF-CN might contribute to the onset and development of neuropathic pain and its role might be partly accomplished by p-CREB.
authors.affiliation: Department of Anesthesiology, The NO.1 Center Hospital Of Bao Ding City, Baoding, 071000, Hebei, China.
authors.forename: Chun-Guang
authors.initials: CG
authors.lastname: Wang
authors.affiliation: Jiangsu Province Key Laboratory of Anesthesiology, Xuzhou Medical College, Xuzhou, 221002, Jiangsu, China.
authors.forename: Si-Yuan
authors.initials: SY
authors.lastname: Song
authors.affiliation: Department of Anesthesiology, The NO.1 Center Hospital Of Bao Ding City, Baoding, 071000, Hebei, China.
authors.forename: Yan-Ling
authors.initials: YL
authors.lastname: Ding
authors.affiliation: Department of Endocrinology, The NO.1 Center Hospital Of Bao Ding City, Baoding, 071000, Hebei, China.
authors.forename: Shu-Qin
authors.initials: SQ
authors.lastname: Guo
authors.affiliation: Department of clinical laboratory, The NO.1 Center Hospital of Bao Ding City, Baoding, 071000, Hebei, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Anesthesiology, The NO.1 Center Hospital Of Bao Ding City, Baoding, 071000, Hebei, China.
authors.forename: Shi
authors.initials: S
authors.lastname: Hao
authors.affiliation: Department of Anesthesiology, The NO.1 Center Hospital Of Bao Ding City, Baoding, 071000, Hebei, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Anesthesiology, The NO.1 Center Hospital Of Bao Ding City, Baoding, 071000, Hebei, China.
authors.forename: Na
authors.initials: N
authors.lastname: Chen
authors.affiliation: Department of Anesthesiology, The NO.1 Center Hospital Of Bao Ding City, Baoding, 071000, Hebei, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Jiangsu Province Key Laboratory of Anesthesiology, Xuzhou Medical College, Xuzhou, 221002, Jiangsu, China.
authors.forename: Li-Cai
authors.initials: LC
authors.lastname: Zhang
mesh_terms: D000818:Animals; D017362:Cyclic AMP Response Element-Binding Protein / Q000134:cerebrospinal fluid; D004789:Enzyme Activation / Q000502:physiology; D006930:Hyperalgesia / Q000134:cerebrospinal fluid / Q000201:enzymology; D008297:Male; D048054:Mitogen-Activated Protein Kinase 7 / Q000134:cerebrospinal fluid* / Q000378:metabolism; D009437:Neuralgia / Q000134:cerebrospinal fluid* / Q000201:enzymology*; D010487:Periaqueductal Gray / Q000201:enzymology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D017362:Cyclic AMP Response Element-Binding Protein; D048054:Mitogen-Activated Protein Kinase 7