title: The effects of Chamaecyparis obtusa essential oil on pain-related behavior and expression of pro-inflammatory cytokines in carrageenan-induced arthritis in rats.
abstract: Chamaecyparis obtusa essential oil (COE) has been widely used to treat allergic diseases and was suggested to exert anti-inflammatory, antioxidant, and antimicrobial effects. This study evaluated the effects of COE on pain-related behavior and pro-inflammatory cytokines in rats with carrageenan (CGN)-induced arthritis. Reduced dynamic weight load on inflamed joint in voluntarily walking rats was used as the behavior test for arthritic pain; 10% COE-treated group was significantly attenuated pain (6-8 h post-CGN injection) compared to VEH (mineral oil)-treated group. In addition, the protein levels of interleukin (IL)-1β, tumor necrosis factor-α, IL-6 (6-8 h), and cyclooxygenase (COX)-2 (8 h) within the synovial membrane, as well as IL-1β, COX-2 (6-8 h), and IL-6 (5-7 h) within the meniscus, of 10% COE-treated group were significantly reduced. The current results implicate that COE has anti-inflammatory and anti-nociceptive effects on arthritis in rats.
authors.affiliation: a Department of Physiology , College of Medicine and Neuroscience Research Institute, Korea University , Seoul , South Korea.
authors.forename: Hye Rim
authors.initials: HR
authors.lastname: Suh
authors.affiliation: a Department of Physiology , College of Medicine and Neuroscience Research Institute, Korea University , Seoul , South Korea.
authors.forename: Hyun Joo
authors.initials: HJ
authors.lastname: Chung
authors.affiliation: a Department of Physiology , College of Medicine and Neuroscience Research Institute, Korea University , Seoul , South Korea.
authors.forename: Eui Ho
authors.initials: EH
authors.lastname: Park
authors.affiliation: a Department of Physiology , College of Medicine and Neuroscience Research Institute, Korea University , Seoul , South Korea.
authors.forename: Sun Wook
authors.initials: SW
authors.lastname: Moon
authors.affiliation: b Department of Forest Welfare , Korea Forest Research Institute , Seoul , South Korea.
authors.forename: Su Jin
authors.initials: SJ
authors.lastname: Park
authors.affiliation: b Department of Forest Welfare , Korea Forest Research Institute , Seoul , South Korea.
authors.forename: Chan Woo
authors.initials: CW
authors.lastname: Park
authors.affiliation: a Department of Physiology , College of Medicine and Neuroscience Research Institute, Korea University , Seoul , South Korea.
authors.forename: Yang In
authors.initials: YI
authors.lastname: Kim
authors.affiliation: a Department of Physiology , College of Medicine and Neuroscience Research Institute, Korea University , Seoul , South Korea.
authors.forename: Hee Chul
authors.initials: HC
authors.lastname: Han
mesh_terms: D000700:Analgesics / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology*; D001169:Arthritis, Experimental / Q000139:chemically induced / Q000188:drug therapy* / Q000503:physiopathology; D002351:Carrageenan; D029780:Chamaecyparis / Q000737:chemistry*; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D053583:Interleukin-1beta / Q000235:genetics / Q000378:metabolism; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism; D008297:Male; D009822:Oils, Volatile / Q000302:isolation & purification / Q000494:pharmacology*; D010146:Pain / Q000139:chemically induced / Q000188:drug therapy* / Q000503:physiopathology; D008517:Phytotherapy / Q000379:methods*; D010936:Plant Extracts / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D013583:Synovial Membrane / Q000187:drug effects / Q000503:physiopathology; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism
chemical_list: D000700:Analgesics; D000893:Anti-Inflammatory Agents; D053583:Interleukin-1beta; D015850:Interleukin-6; D009822:Oils, Volatile; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D002351:Carrageenan; D051546:Cyclooxygenase 2; C489691:Ptgs2 protein, rat
keywords: Chamaecyparis obtusa essential oil; carrageenan-induced arthritis; dynamic weight load; pro-inflammatory cytokines