title: MvaT Family Proteins Encoded on IncP-7 Plasmid pCAR1 and the Host Chromosome Regulate the Host Transcriptome Cooperatively but Differently.
abstract: MvaT proteins are members of the H-NS family of proteins in pseudomonads. The IncP-7 conjugative plasmid pCAR1 carries an mvaT-homologous gene, pmr. In Pseudomonas putida KT2440 bearing pCAR1, pmr and the chromosomally carried homologous genes, turA and turB, are transcribed at high levels, and Pmr interacts with TurA and TurB in vitro. In the present study, we clarified how the three MvaT proteins regulate the transcriptome of P. putida KT2440(pCAR1). Analyses performed by a modified chromatin immunoprecipitation assay with microarray technology (ChIP-chip) suggested that the binding regions of Pmr, TurA, and TurB in the P. putida KT2440(pCAR1) genome are almost identical; nevertheless, transcriptomic analyses using mutants with deletions of the genes encoding the MvaT proteins during the log and early stationary growth phases clearly suggested that their regulons were different. Indeed, significant regulon dissimilarity was found between Pmr and the other two proteins. Transcription of a larger number of genes was affected by Pmr deletion during early stationary phase than during log phase, suggesting that Pmr ameliorates the effects of pCAR1 on host fitness more effectively during the early stationary phase. Alternatively, the similarity of the TurA and TurB regulons implied that they might play complementary roles as global transcriptional regulators in response to plasmid carriage.
authors.affiliation: Biotechnology Research Center, The University of Tokyo, Tokyo, Japan Agricultural Bioinformatics Research Unit, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
authors.forename: Choong-Soo
authors.initials: CS
authors.lastname: Yun
authors.affiliation: Biotechnology Research Center, The University of Tokyo, Tokyo, Japan Biotechnology Research Center and Department of Biotechnology, Toyama Prefectural University, Imizu, Toyama, Japan.
authors.forename: Yurika
authors.initials: Y
authors.lastname: Takahashi
authors.affiliation: Biotechnology Research Center, The University of Tokyo, Tokyo, Japan Applied Chemistry and Biochemical Engineering Course, Department of Engineering, Graduate School of Integrated Science and Technology, Shizuoka University, Hamamatsu, Shizuoka, Japan.
authors.forename: Masaki
authors.initials: M
authors.lastname: Shintani
authors.affiliation: Biotechnology Research Center, The University of Tokyo, Tokyo, Japan.
authors.forename: Toshiharu
authors.initials: T
authors.lastname: Takeda
authors.affiliation: Biotechnology Research Center, The University of Tokyo, Tokyo, Japan.
authors.forename: Chiho
authors.initials: C
authors.lastname: Suzuki-Minakuchi
authors.affiliation: Biotechnology Research Center, The University of Tokyo, Tokyo, Japan.
authors.forename: Kazunori
authors.initials: K
authors.lastname: Okada
authors.affiliation: Biotechnology Research Center, The University of Tokyo, Tokyo, Japan.
authors.forename: Hisakazu
authors.initials: H
authors.lastname: Yamane
authors.affiliation: Biotechnology Research Center, The University of Tokyo, Tokyo, Japan Agricultural Bioinformatics Research Unit, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan anojiri@mail.ecc.u-tokyo.ac.jp.
authors.forename: Hideaki
authors.initials: H
authors.lastname: Nojiri
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D002876:Chromosomes, Bacterial / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D056084:Genetic Fitness; D016680:Genome, Bacterial; D047468:Immunoprecipitation; D046228:Microarray Analysis; D010957:Plasmids / Q000235:genetics; D016958:Pseudomonas putida / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D018085:Regulon; D017384:Sequence Deletion; D015534:Trans-Activators / Q000235:genetics*; D059467:Transcriptome
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; C111692:MvaT protein, Pseudomonas; D015534:Trans-Activators