title: Complete genome sequence of a strain of Actinidia virus X detected in Ribes nigrum cv. Baldwin showing unusual symptoms.
abstract: A Ribes-infecting strain of the potexvirus Actinidia virus X (AVX-RV3124) was isolated from black currant plants (Ribes nigrum cv. Baldwin, accession 3124-03D1) showing symptoms of leaf chlorosis and deformity. This is the first description of the complete genome sequence of an isolate of this virus and the first detection of a potexvirus in Ribes. The genome of AVX-RV3124 consists of 6,888 nucleotides (nt) excluding the poly(A) tail at the 3' terminus. When AVX-RV3124 was compared to the available sequence of the AVX isolate in GenBank (accession no. KC568202), two large indel events (72 nt and 33 nt) were identified in the replicase coding region of RV3124. Evidence of recombination was detected upstream of the 3' terminus of the replicase gene of both virus isolates, providing further evidence of a common origin.
authors.affiliation: Sidney Laboratory, Canadian Food Inspection Agency, 8801 East Saanich Road, North Saanich, BC, V8L 1H3, Canada. Delano.James@inspection.gc.ca.
authors.forename: Delano
authors.initials: D
authors.lastname: James
authors.affiliation: Sidney Laboratory, Canadian Food Inspection Agency, 8801 East Saanich Road, North Saanich, BC, V8L 1H3, Canada.
authors.forename: James
authors.initials: J
authors.lastname: Phelan
mesh_terms: D016679:Genome, Viral; D054643:INDEL Mutation; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000821:virology*; D017863:Potexvirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D012367:RNA, Viral / Q000235:genetics*; D011995:Recombination, Genetic; D031965:Ribes / Q000821:virology*; D017422:Sequence Analysis, DNA
chemical_list: D012367:RNA, Viral