title: Cellular Composition of the Spleen and Changes in Splenic Lysosomes in the Dynamics of Dyslipidemia in Mice Caused by Repeated Administration of Poloxamer 407.
abstract: We studied the effect of dyslipidemia induced by poloxamer 407 (300 mg/kg twice a week for 30 days) on cellular composition of the spleen and splenocyte lysosomes in mice. Changes in blood lipid profile included elevated concentrations of total cholesterol, aterogenic LDL, and triglycerides most pronounced in 24 h after the last poloxamer 407 injection; gradual normalization of lipid profile was observed in 4 days (except triglycerides) and 10 days. The most pronounced changes in the spleen (increase in organ weight and number of cells, inhibition in apoptosis, and reduced accumulation of vital dye acridine orange in lysosomes) were detected on day 4; on day 10, the indices returned to normal. Cathepsin D activity in the spleen also increased at these terms. The relationship between changes in the cellular composition of the spleen and dynamics of serum lipid profile in mice in dyslipidemia caused by repeated administrations of relatively low doses of poloxamer 407 is discussed.
authors.affiliation: Research Institute of Physiology and Basic Medicine, Siberian Division of Russian Academy of Medical Sciences, Novosibirsk, Russia.
authors.forename: N V
authors.initials: NV
authors.lastname: Goncharova
authors.affiliation: Research Institute of Physiology and Basic Medicine, Siberian Division of Russian Academy of Medical Sciences, Novosibirsk, Russia.
authors.forename: A V
authors.initials: AV
authors.lastname: Shurlygina
authors.affiliation: Research Institute of Physiology and Basic Medicine, Siberian Division of Russian Academy of Medical Sciences, Novosibirsk, Russia.
authors.forename: E V
authors.initials: EV
authors.lastname: Mel'nikova
authors.affiliation: Research Institute of Physiology and Basic Medicine, Siberian Division of Russian Academy of Medical Sciences, Novosibirsk, Russia.
authors.forename: O L
authors.initials: OL
authors.lastname: Karmatskikh
authors.affiliation: Research Institute of Physiology and Basic Medicine, Siberian Division of Russian Academy of Medical Sciences, Novosibirsk, Russia.
authors.forename: P A
authors.initials: PA
authors.lastname: Avrorov
authors.affiliation: Research Institute of Physiology and Basic Medicine, Siberian Division of Russian Academy of Medical Sciences, Novosibirsk, Russia.
authors.forename: K V
authors.initials: KV
authors.lastname: Loktev
authors.affiliation: Research Institute of Physiology and Basic Medicine, Siberian Division of Russian Academy of Medical Sciences, Novosibirsk, Russia. t.a.korolenko@physiol.ru.
authors.forename: T A
authors.initials: TA
authors.lastname: Korolenko
mesh_terms: D000818:Animals; D002402:Cathepsin D / Q000032:analysis; D002784:Cholesterol / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood; D050171:Dyslipidemias / Q000097:blood / Q000139:chemically induced / Q000473:pathology*; D008247:Lysosomes / Q000187:drug effects / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D009929:Organ Size / Q000187:drug effects; D020442:Poloxamer / Q000633:toxicity*; D013154:Spleen / Q000187:drug effects / Q000201:enzymology / Q000473:pathology* / Q000648:ultrastructure; D014280:Triglycerides / Q000097:blood
chemical_list: D008078:Cholesterol, LDL; D014280:Triglycerides; D020442:Poloxamer; D002784:Cholesterol; D002402:Cathepsin D; C533923:Ctsd protein, mouse
keywords: dyslipidemia; lysosomes; poloxamer 407; spleen