title: [Comparative study on preparation of Polygoni Multiflori Radix based on hepatotoxic bioassay].
abstract: Toxicity of different processed was evaluated Polygoni Multiflori Radix by determining the hepatotoxic potency for selecting processing technology. Process Polygoni Multiflori Radix using high pressure steamed, Black Bean high pressure steamed, atmospheric steamed for different time. Using normal human hepatocytes (L02) as evaluation model, hepatotoxic potency as index to evaluate hepatotoxic potency of different processed Polygoni Multiflori Radix. Analysis chemical composition of some processed products by UPLC-MS. Hepatotoxic bioassay method cloud evaluate the toxicity of different Polygoni Multiflori Radix samples. Different processing methods can reduce the toxicity of Polygoni Multiflori Radix, high pressure steamed three hours attenuated was better. Different processing methods have different effects on chemical constituents of Polygoni Multiflori Radix. Comparing with crude sample, the contents of gallic acid, 2,3,5,4-tetrahydroxyl diphenylethylene-2-O-glucoside, emodin-8-O-beta glucoside and emodin were decreased in processed products with 3 kinds of different methods. The change trend of 2,3,5,4-tetrahydroxyl diphenylethylene-2-O-glucoside content was similar with hepatotoxic potency. Different processing methods can reduce the toxicity of Polygoni Multiflori Radix. Processing methods and time attenuated obvious impact on toxicity. Recommended further research on the attehuated standard control of Polygoni Multiflori Radix concocted.
authors.forename: Zhi-jie
authors.initials: ZJ
authors.lastname: Ma
authors.forename: Xiao-fei
authors.initials: XF
authors.lastname: Li
authors.forename: Yang
authors.initials: Y
authors.lastname: Lv
authors.forename: Bing-qian
authors.initials: BQ
authors.lastname: Jiang
authors.forename: Yan-ling
authors.initials: YL
authors.lastname: Zhao
authors.forename: Ya-ming
authors.initials: YM
authors.lastname: Zhang
authors.forename: Kui-jun
authors.initials: KJ
authors.lastname: Zhao
authors.forename: Jia-bo
authors.initials: JB
authors.lastname: Wang
authors.forename: Xiao-he
authors.initials: XH
authors.lastname: Xiao
mesh_terms: D001681:Biological Assay; D002460:Cell Line; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000633:toxicity*; D000068676:Fallopia multiflora / Q000737:chemistry* / Q000633:toxicity; D022781:Hepatocytes / Q000187:drug effects*; D006801:Humans; D018517:Plant Roots / Q000737:chemistry* / Q000633:toxicity
chemical_list: D004365:Drugs, Chinese Herbal