title: Rocket and Two Dimensional Immunoelectrophoresis in Diagnosis of Caprine Brucellosis.
abstract: BACKGROUND
  Brucellosis is a major bacterial zoonosis of global importance with the causative organisms of Gram-negative facultative intracellular pathogens. The aims of this study were to standardize two immunoelectrophoretic techniques, rocket and cross immunoelectrophoresis, and compare their results with other conventional serodiagnostic tests.
  
  
  METHODS
  Sera from 15 sheep, without any history of brucellosis vaccination, infected with Brucella melitensis M16 subcutaneously, were employed in a comparison of culture, precipitating, and immunoelectrophoretic tests. A 125 days serologic follow-up was performed after the infection was started. As a reference, these tests also done in the five healthy sheep.
  
  
  RESULTS
  The results obtained with the rocket immunoelectrophoresis test correlated very well with those of the cross immunoelectrophoresis, whereas results of other tests such as culture, Rose Bengal, standard tube agglutination and 2-mercaptoethanol seruagglutination tests were inferior.
  
  
  CONCLUSIONS
  As agglutination test shows cross reaction and a prozone phenomenon, and in blood culture, the bacteria is not always detectable, so they are time consuming rocket and cross immunoelectrophoresis are recommended because their results can be obtained in a shorter time.
authors.affiliation: Stem Cell and Transgenic Technology Research Center, Dept. of Pathology, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Davood
authors.initials: D
authors.lastname: Mehrabani
authors.affiliation: Dept. of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Gholami
authors.affiliation: Dept. of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Jamshid
authors.initials: J
authors.lastname: Kohanteb
authors.affiliation: Gasteroenterohepatology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Masood
authors.initials: M
authors.lastname: Sepehrimanesh
authors.affiliation: Razi Vaccine and Serum Research Institute, Shiraz, Iran.
authors.forename: Seyed Mohammad Hossein
authors.initials: SM
authors.lastname: Hosseini
keywords: Agglutination; Brucellosis; Immunoelectrophoresis; Immunological diagnosis; Zoonosis