title: Dynamics of Lgr6⁺ Progenitor Cells in the Hair Follicle, Sebaceous Gland, and Interfollicular Epidermis.
abstract: The dynamics and interactions between stem cell pools in the hair follicle (HF), sebaceous gland (SG), and interfollicular epidermis (IFE) of murine skin are still poorly understood. In this study, we used multicolor lineage tracing to mark Lgr6⁺ -expressing basal cells in the HF isthmus, SG, and IFE.We show that these Lgr6⁺ cells constitute long-term self-renewing populations within each compartment in adult skin. Quantitative analysis of clonal dynamics revealed that the Lgr6⁺ progenitor cells compete neutrally in the IFE, isthmus, and SG, indicating population asymmetry as the underlying mode of tissue renewal. Transcriptional profiling of Lgr6⁺ and Lgr6⁺ cells did not reveal a distinct Lgr6⁺ -associated gene expression signature, raising the question of whether Lgr6⁺ expression requires extrinsic niche signals. Our results elucidate the interrelation and behavior of Lgr6⁺ populations in the IFE, HF, and SG and suggest population asymmetry as a common mechanism for homeostasis in several epithelial skin compartments.
authors.affiliation: Karolinska Institutet, Department of Biosciences and Nutrition and Center for Innovative Medicine, Novum, 141 83 Huddinge, Sweden.
authors.forename: Anja
authors.initials: A
authors.lastname: Füllgrabe
authors.affiliation: Karolinska Institutet, Department of Biosciences and Nutrition and Center for Innovative Medicine, Novum, 141 83 Huddinge, Sweden.
authors.forename: Simon
authors.initials: S
authors.lastname: Joost
authors.affiliation: Karolinska Institutet, Department of Biosciences and Nutrition and Center for Innovative Medicine, Novum, 141 83 Huddinge, Sweden.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Are
authors.affiliation: Karolinska Institutet, Department of Biosciences and Nutrition and Center for Innovative Medicine, Novum, 141 83 Huddinge, Sweden.
authors.forename: Tina
authors.initials: T
authors.lastname: Jacob
authors.affiliation: Karolinska Institutet, Department of Biosciences and Nutrition and Center for Innovative Medicine, Novum, 141 83 Huddinge, Sweden.
authors.forename: Unnikrishnan
authors.initials: U
authors.lastname: Sivan
authors.affiliation: Hubrecht Institute-KNAW (Royal Netherlands Academy of Arts and Sciences) and University Medical Center Utrecht, Uppsalalaan 8, 3584 Utrecht, the Netherlands.
authors.forename: Andrea
authors.initials: A
authors.lastname: Haegebarth
authors.affiliation: Karolinska Institutet, Department of Medical Biochemistry and Biophysics, Scheeles väg 2, 171 77 Stockholm, Sweden.
authors.forename: Sten
authors.initials: S
authors.lastname: Linnarsson
authors.affiliation: The Wellcome Trust/Cancer Research UK Gurdon Institute, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK; Cavendish Laboratory, Department of Physics, J. J. Thomson Avenue, University of Cambridge, Cambridge CB3 0HE, UK; Wellcome Trust-Medical Research Council Stem Cell Institute, University of Cambridge, Cambridge CB2 1QR, UK.
authors.forename: Benjamin D
authors.initials: BD
authors.lastname: Simons
authors.affiliation: Hubrecht Institute-KNAW (Royal Netherlands Academy of Arts and Sciences) and University Medical Center Utrecht, Uppsalalaan 8, 3584 Utrecht, the Netherlands.
authors.forename: Hans
authors.initials: H
authors.lastname: Clevers
authors.affiliation: Karolinska Institutet, Department of Biosciences and Nutrition and Center for Innovative Medicine, Novum, 141 83 Huddinge, Sweden.
authors.forename: Rune
authors.initials: R
authors.lastname: Toftgård
authors.affiliation: Karolinska Institutet, Department of Biosciences and Nutrition and Center for Innovative Medicine, Novum, 141 83 Huddinge, Sweden. Electronic address: maria.kasper@ki.se.
authors.forename: Maria
authors.initials: M
authors.lastname: Kasper
mesh_terms: D053687:Adult Stem Cells / Q000166:cytology* / Q000378:metabolism; D000818:Animals; D000066673:Cell Self Renewal; D002478:Cells, Cultured; D018859:Hair Follicle / Q000166:cytology*; D015603:Keratinocytes / Q000166:cytology / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000378:metabolism; D012627:Sebaceous Glands / Q000166:cytology*; D055153:Stem Cell Niche; D059467:Transcriptome
chemical_list: C548022:Lgr6 protein, mouse; D043562:Receptors, G-Protein-Coupled