title: Genetic Susceptibility to Cardiac and Digestive Clinical Forms of Chronic Chagas Disease: Involvement of the CCR5 59029 A/G Polymorphism.
abstract: The clinical manifestations of chronic Chagas disease include the cardiac form of the disease and the digestive form. Not all the factors that act in the variable clinical course of this disease are known. This study investigated whether the CCR5Δ32 (rs333) and CCR5 59029 A/G (promoter region--rs1799987) polymorphisms of the CCR5 gene are associated with different clinical forms of chronic Chagas disease and with the severity of left ventricular systolic dysfunction in patients with chronic Chagas heart disease (CCHD). The antibodies anti-T. cruzi were identified by ELISA. PCR and PCR-RFLP were used to identify the CCR5Δ32 and CCR5 59029 A/G polymorphisms. The chi-square test was used to compare variables between groups. There was a higher frequency of the AA genotype in patients with CCHD compared with patients with the digestive form of the disease and the control group. The results also showed a high frequency of the AG genotype in patients with the digestive form of the disease compared to the other groups. The results of this study show that the CCR5Δ32 polymorphism does not seem to influence the different clinical manifestations of Chagas disease but there is involvement of the CCR5 59029 A/G polymorphism in susceptibility to the different forms of chronic Chagas disease. Besides, these polymorphisms do not influence left ventricular systolic dysfunction in patients with CCHD.
authors.affiliation: Laboratório de Imunogenética, Departamento de Biologia Molecular, Faculdade de Medicina de São José do Rio Preto, Avenida Brigadeiro Faria Lima, 5416, 15090-000, São José do Rio Preto, SP, Brazil.
authors.forename: Amanda Priscila
authors.initials: AP
authors.lastname: de Oliveira
authors.affiliation: Laboratório de Imunogenética, Departamento de Biologia Molecular, Faculdade de Medicina de São José do Rio Preto, Avenida Brigadeiro Faria Lima, 5416, 15090-000, São José do Rio Preto, SP, Brazil.
authors.forename: Cássia Rubia
authors.initials: CR
authors.lastname: Bernardo
authors.affiliation: Laboratório de Imunogenética, Departamento de Biologia Molecular, Faculdade de Medicina de São José do Rio Preto, Avenida Brigadeiro Faria Lima, 5416, 15090-000, São José do Rio Preto, SP, Brazil.
authors.forename: Ana Vitória da Silveira
authors.initials: AV
authors.lastname: Camargo
authors.affiliation: Departamento de Cirurgia, Faculdade de Medicina de São José do Rio Preto, Avenida Brigadeiro Faria Lima, 5416, 15090-000, São José do Rio Preto, SP, Brazil.
authors.forename: Luiz Sérgio
authors.initials: LS
authors.lastname: Ronchi
authors.affiliation: Departamento de Cirurgia, Faculdade de Medicina de São José do Rio Preto, Avenida Brigadeiro Faria Lima, 5416, 15090-000, São José do Rio Preto, SP, Brazil.|Departamento de Cirurgia Geral, Fundação Faculdade Regional de Medicina-Hospital de Base, Avenida Brigadeiro Faria Lima, 5544, 15090-000, São José do Rio Preto, SP, Brazil.
authors.forename: Aldenis Albaneze
authors.initials: AA
authors.lastname: Borim
authors.affiliation: Laboratório de Imunogenética, Departamento de Biologia Molecular, Faculdade de Medicina de São José do Rio Preto, Avenida Brigadeiro Faria Lima, 5416, 15090-000, São José do Rio Preto, SP, Brazil.
authors.forename: Cinara Cássia Brandão
authors.initials: CC
authors.lastname: de Mattos
authors.affiliation: Departamento de Cirurgia, Faculdade de Medicina de São José do Rio Preto, Avenida Brigadeiro Faria Lima, 5416, 15090-000, São José do Rio Preto, SP, Brazil.|Departamento de Cirurgia Geral, Fundação Faculdade Regional de Medicina-Hospital de Base, Avenida Brigadeiro Faria Lima, 5544, 15090-000, São José do Rio Preto, SP, Brazil.
authors.forename: Eumildo
authors.initials: E
authors.lastname: de Campos Júnior
authors.affiliation: Departamento de Epidemiologia e Saúde Coletiva, Faculdade de Medicina de São José do Rio Preto, Avenida Brigadeiro Faria Lima, 5416, 15090-000, São José do Rio Preto, SP, Brazil.
authors.forename: Lílian
authors.initials: L
authors.lastname: Castiglioni
authors.affiliation: Departamento de Cirurgia, Faculdade de Medicina de São José do Rio Preto, Avenida Brigadeiro Faria Lima, 5416, 15090-000, São José do Rio Preto, SP, Brazil.|Departamento de Cirurgia Geral, Fundação Faculdade Regional de Medicina-Hospital de Base, Avenida Brigadeiro Faria Lima, 5544, 15090-000, São José do Rio Preto, SP, Brazil.
authors.forename: João Gomes
authors.initials: JG
authors.lastname: Netinho
authors.affiliation: Centro de Investigação de Microrganismos, Departamento de Doenças Dermatológicas, Infecciosas e Parasitárias, Faculdade de Medicina de São José do Rio Preto, Avenida Brigadeiro Faria Lima, 5416, 15090-000, São José do Rio Preto, SP, Brazil.
authors.forename: Carlos Eugênio
authors.initials: CE
authors.lastname: Cavasini
authors.affiliation: Departamento de Cardiologia e Cirurgia Cardiovascular, Faculdade de Medicina de São José do Rio Preto, Avenida Brigadeiro Faria Lima, 5416, 15090-000, São José do Rio Preto, SP, Brazil.
authors.forename: Reinaldo Bulgarelli
authors.initials: RB
authors.lastname: Bestetti
authors.affiliation: Laboratório de Imunogenética, Departamento de Biologia Molecular, Faculdade de Medicina de São José do Rio Preto, Avenida Brigadeiro Faria Lima, 5416, 15090-000, São José do Rio Preto, SP, Brazil.
authors.forename: Luiz Carlos
authors.initials: LC
authors.lastname: de Mattos
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D002598:Chagas Cardiomyopathy / Q000235:genetics* / Q000503:physiopathology; D014355:Chagas Disease / Q000235:genetics* / Q000503:physiopathology; D002908:Chronic Disease; D004066:Digestive System Diseases / Q000235:genetics*; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D005799:Genes, Dominant; D020022:Genetic Predisposition to Disease; D006352:Heart Ventricles / Q000503:physiopathology; D006801:Humans; D040582:Inheritance Patterns / Q000235:genetics; D008297:Male; D008875:Middle Aged; D008957:Models, Genetic; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D019713:Receptors, CCR5 / Q000235:genetics*; D013599:Systole
chemical_list: C584933:CCR5 protein, human; D019713:Receptors, CCR5