title: HIV-1 and two avian retroviral 5' untranslated regions bind orthologous human and chicken RNA binding proteins.
abstract: Essential host cofactors in retrovirus replication bind cis-acting sequences in the 5'untranslated region (UTR). Although host RBPs are crucial to all aspects of virus biology, elucidating their roles in replication remains a challenge to the field. Here RNA affinity-coupled-proteomics generated a comprehensive, unbiased inventory of human and avian RNA binding proteins (RBPs) co-isolating with 5'UTRs of HIV-1, spleen necrosis virus and Rous sarcoma virus. Applying stringent biochemical and statistical criteria, we identified 185 RBP; 122 were previously implicated in retrovirus biology and 63 are new to the 5'UTR proteome. RNA electrophoretic mobility assays investigated paralogs present in the common ancestor of vertebrates and one hnRNP was identified as a central node to the biological process-anchored networks of HIV-1, SNV, and RSV 5' UTR-proteomes. This comprehensive view of the host constituents of retroviral RNPs is broadly applicable to investigation of viral replication and antiviral response in both human and avian cell lineages.
authors.affiliation: Department of Medicine, Penn State College of Medicine, 500 University Drive, Hershey, PA 17033, USA. Electronic address: mss31@psu.edu.
authors.forename: Matthew
authors.initials: M
authors.lastname: Stake
authors.affiliation: School of Biotechnology, Gautam Buddha University, Greater Noida, 201312, India. Electronic address: deepali@gbu.ac.in.
authors.forename: Deepali
authors.initials: D
authors.lastname: Singh
authors.affiliation: Department Veterinary & Biomedical Sciences, University of Minnesota, 205 VSB, 1971 Commonwealth Avenue, Saint Paul, MN 55108. Electronic address: gsingh@umn.edu.
authors.forename: Gatikrushna
authors.initials: G
authors.lastname: Singh
authors.affiliation: Department of Veterinary Biosciences, Center for Retrovirus Research, Center for RNA Biology, Comprehensive Cancer Center, Ohio State University, Columbus, OH, USA. Electronic address: hernandez.1111@osu.edu.
authors.forename: J
authors.initials: J
authors.lastname: Marcela Hernandez
authors.affiliation: Department of Medicine, Penn State College of Medicine, 500 University Drive, Hershey, PA 17033, USA. Electronic address: rjk297@psu.edu.
authors.forename: Rebecca
authors.initials: R
authors.lastname: Kaddis Maldonado
authors.affiliation: Department of Medicine, Penn State College of Medicine, 500 University Drive, Hershey, PA 17033, USA; Department Microbiology & Immunology, Penn State College of Medicine, 500 University Drive, Hershey, PA 17033, USA. Electronic address: lparent@psu.edu.
authors.forename: Leslie J
authors.initials: LJ
authors.lastname: Parent
authors.affiliation: Department Veterinary & Biomedical Sciences, University of Minnesota, 205 VSB, 1971 Commonwealth Avenue, Saint Paul, MN 55108. Electronic address: kbl@umn.edu.
authors.forename: Kathleen
authors.initials: K
authors.lastname: Boris-Lawrie
mesh_terms: D020121:5' Untranslated Regions; D000595:Amino Acid Sequence; D000818:Animals; D002645:Chickens; D015658:HIV Infections / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D015497:HIV-1 / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D011201:Poultry Diseases / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D012190:Retroviridae / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D012192:Retroviridae Infections / Q000235:genetics / Q000378:metabolism* / Q000821:virology
chemical_list: D020121:5' Untranslated Regions; D012367:RNA, Viral; D016601:RNA-Binding Proteins
keywords: Post-transcriptional control; RNA recognition motif; Virus–host interaction; cis-Acting replication sequences; hnRNPs