title: EMDataBank unified data resource for 3DEM.
abstract: Three-dimensional Electron Microscopy (3DEM) has become a key experimental method in structural biology for a broad spectrum of biological specimens from molecules to cells. The EMDataBank project provides a unified portal for deposition, retrieval and analysis of 3DEM density maps, atomic models and associated metadata (emdatabank.org). We provide here an overview of the rapidly growing 3DEM structural data archives, which include maps in EM Data Bank and map-derived models in the Protein Data Bank. In addition, we describe progress and approaches toward development of validation protocols and methods, working with the scientific community, in order to create a validation pipeline for 3DEM data.
authors.affiliation: Department of Chemistry and Chemical Biology and Research Collaboratory for Structural Bioinformatics, Rutgers, The State University of New Jersey, 610 Taylor Road Piscataway, NJ 08854, USA cathy.lawson@rutgers.edu.
authors.forename: Catherine L
authors.initials: CL
authors.lastname: Lawson
authors.affiliation: Protein Data Bank in Europe, European Molecular Biology Laboratory, European Bioinformatics Institute, Wellcome Genome Campus, Hinxton, Cambridge CB10 1SD, UK ardan@ebi.ac.uk.
authors.forename: Ardan
authors.initials: A
authors.lastname: Patwardhan
authors.affiliation: Verna and Marrs McLean Department of Biochemistry & Molecular Biology, National Center for Macromolecular Imaging, Baylor College of Medicine, 1 Baylor Plaza, Houston, TX 70030, USA.
authors.forename: Matthew L
authors.initials: ML
authors.lastname: Baker
authors.affiliation: Verna and Marrs McLean Department of Biochemistry & Molecular Biology, National Center for Macromolecular Imaging, Baylor College of Medicine, 1 Baylor Plaza, Houston, TX 70030, USA.
authors.forename: Corey
authors.initials: C
authors.lastname: Hryc
authors.affiliation: Protein Data Bank in Europe, European Molecular Biology Laboratory, European Bioinformatics Institute, Wellcome Genome Campus, Hinxton, Cambridge CB10 1SD, UK.
authors.forename: Eduardo Sanz
authors.initials: ES
authors.lastname: Garcia
authors.affiliation: Department of Chemistry and Chemical Biology and Research Collaboratory for Structural Bioinformatics, Rutgers, The State University of New Jersey, 610 Taylor Road Piscataway, NJ 08854, USA.
authors.forename: Brian P
authors.initials: BP
authors.lastname: Hudson
authors.affiliation: Protein Data Bank in Europe, European Molecular Biology Laboratory, European Bioinformatics Institute, Wellcome Genome Campus, Hinxton, Cambridge CB10 1SD, UK.
authors.forename: Ingvar
authors.initials: I
authors.lastname: Lagerstedt
authors.affiliation: Verna and Marrs McLean Department of Biochemistry & Molecular Biology, National Center for Macromolecular Imaging, Baylor College of Medicine, 1 Baylor Plaza, Houston, TX 70030, USA.
authors.forename: Steven J
authors.initials: SJ
authors.lastname: Ludtke
authors.affiliation: Verna and Marrs McLean Department of Biochemistry & Molecular Biology, National Center for Macromolecular Imaging, Baylor College of Medicine, 1 Baylor Plaza, Houston, TX 70030, USA.
authors.forename: Grigore
authors.initials: G
authors.lastname: Pintilie
authors.affiliation: Department of Chemistry and Chemical Biology and Research Collaboratory for Structural Bioinformatics, Rutgers, The State University of New Jersey, 610 Taylor Road Piscataway, NJ 08854, USA.
authors.forename: Raul
authors.initials: R
authors.lastname: Sala
authors.affiliation: Department of Chemistry and Chemical Biology and Research Collaboratory for Structural Bioinformatics, Rutgers, The State University of New Jersey, 610 Taylor Road Piscataway, NJ 08854, USA.
authors.forename: John D
authors.initials: JD
authors.lastname: Westbrook
authors.affiliation: Department of Chemistry and Chemical Biology and Research Collaboratory for Structural Bioinformatics, Rutgers, The State University of New Jersey, 610 Taylor Road Piscataway, NJ 08854, USA.
authors.forename: Helen M
authors.initials: HM
authors.lastname: Berman
authors.affiliation: Protein Data Bank in Europe, European Molecular Biology Laboratory, European Bioinformatics Institute, Wellcome Genome Campus, Hinxton, Cambridge CB10 1SD, UK.
authors.forename: Gerard J
authors.initials: GJ
authors.lastname: Kleywegt
authors.affiliation: Verna and Marrs McLean Department of Biochemistry & Molecular Biology, National Center for Macromolecular Imaging, Baylor College of Medicine, 1 Baylor Plaza, Houston, TX 70030, USA wah@bcm.edu.
authors.forename: Wah
authors.initials: W
authors.lastname: Chiu
mesh_terms: D016208:Databases, Factual; D030562:Databases, Protein; D021621:Imaging, Three-Dimensional; D046911:Macromolecular Substances / Q000737:chemistry*; D008854:Microscopy, Electron; D008958:Models, Molecular; D011506:Proteins / Q000737:chemistry
chemical_list: D046911:Macromolecular Substances; D011506:Proteins