title: [Development of an Immunochromatographic Test System for the Detection of Helicobacter pylori Antigens].
abstract: A test system based on immunochromatography in the sandwich format and intended for express detection of Helicobacter pylori antigens has been developed. Contact of a sample with a test strip coated with immunochemical reagents triggers the movement of the liquid along the membrane components of the test strip, immunochemical interactions, and the formation of detection zones stained by gold nanoparticles. The concentration and kinetic dependences of the immunochemical interactions have been characterized. The reagent and membrane composition of the test system has been selected to provide a minimal detection limit. The detection of H. pylori cell wall antigens at concentrations as low as 0.3 μg/mL in aqueous solution and a suspension of a clinical sample of feces has been demonstrated; the assay duration was 10 minutes. Staining enhancement by the addition of silver salts allowed for a further reduction of the detection limit to 0.03 μg/mL. The developed test system can be used for field diagnostics.
authors.forename: N A
authors.initials: NA
authors.lastname: Byzova
authors.forename: A V
authors.initials: AV
authors.lastname: Zherdev
authors.forename: P G
authors.initials: PG
authors.lastname: Sveshnikov
authors.forename: E G
authors.initials: EG
authors.lastname: Sadykhov
authors.forename: B B
authors.initials: BB
authors.lastname: Dzantiev
mesh_terms: D000942:Antigens, Bacterial / Q000276:immunology / Q000302:isolation & purification*; D015374:Biosensing Techniques; D002846:Chromatography, Affinity / Q000379:methods*; D006046:Gold; D016481:Helicobacter Infections / Q000175:diagnosis* / Q000276:immunology; D016480:Helicobacter pylori / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry
chemical_list: D000942:Antigens, Bacterial; D006046:Gold