title: ISOLATION AND MOLECULAR IDENTIFICATION OF POTENTIALLY PATHOGENIC Escherichia coli AND Campylobacter jejuni IN FERAL PIGEONS FROM AN URBAN AREA IN THE CITY OF LIMA, PERU.
abstract: Feral pigeons (Columbia livia) live in close contact with humans and other animals. They can transmit potentially pathogenic and zoonotic agents. The objective of this study was to isolate and detect strains of diarrheagenic Escherichia coli and Campylobacter jejuni of urban feral pigeons from an area of Lima, Peru. Fresh dropping samples from urban parks were collected for microbiological isolation of E. coli strains in selective agar, and Campylobacter by filtration method. Molecular identification of diarrheagenic pathotypes of E.coli and Campylobacter jejuni was performed by PCR. Twenty-two parks were sampled and 16 colonies of Campylobacter spp. were isolated. The 100% of isolates were identified as Campylobacter jejuni. Furthermore, 102 colonies of E. coli were isolated and the 5.88% resulted as Enteropathogenic (EPEC) type and 0.98% as Shiga toxin-producing E. coli (STEC). The urban feral pigeons of Lima in Peru can act as a reservoir or carriers of zoonotic potentially pathogenic enteric agents.
authors.affiliation: Facultad de Veterinaria y Zootecnia, Universidad Peruana Cayetano Heredia, Lima, Perú
authors.forename: Moisés
authors.initials: M
authors.lastname: Caballero
authors.affiliation: Facultad de Veterinaria y Zootecnia, Universidad Peruana Cayetano Heredia, Lima, Perú
authors.forename: Isabel
authors.initials: I
authors.lastname: Rivera
authors.affiliation: Facultad de Veterinaria y Zootecnia, Universidad Peruana Cayetano Heredia, Lima, Perú
authors.forename: Luis M
authors.initials: LM
authors.lastname: Jara
authors.affiliation: Faculdade de Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, SP, Brasil.
authors.forename: Francisco M
authors.initials: FM
authors.lastname: Ulloa-Stanojlovic
authors.affiliation: Facultad de Veterinaria y Zootecnia, Universidad Peruana Cayetano Heredia, Lima, Perú
authors.forename: Carlos
authors.initials: C
authors.lastname: Shiva
mesh_terms: D000818:Animals; D016123:Campylobacter jejuni / Q000302:isolation & purification* / Q000472:pathogenicity; D010856:Columbidae / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000302:isolation & purification* / Q000472:pathogenicity; D005243:Feces / Q000382:microbiology; D010568:Peru; D016133:Polymerase Chain Reaction; D014505:Urban Population; D037521:Virulence Factors / Q000302:isolation & purification
chemical_list: D004269:DNA, Bacterial; D037521:Virulence Factors