title: Can dendritic cells improve whole cancer cell vaccines based on immunogenically killed cancer cells?
abstract: Immunogenic cell death (ICD) offers interesting opportunities in cancer cell (CC) vaccine manufacture, as it increases the immunogenicity of the dead CC. Furthermore, fusion of CCs with dendritic cells (DCs) is considered a superior method for generating whole CC vaccines. Therefore, in this work, we determined in naive mice whether immunogenically killed CCs per se (CC vaccine) elicit an antitumoral immune response different from the response observed when immunogenically killed CCs are associated with DCs through fusion (fusion vaccine) or through co-incubation (co-incubation vaccine). After tumor inoculation, the type of immune response in the prophylactically vaccinated mice differed between the groups. In more detail, fusion vaccines elicited a humoral anticancer response, whereas the co-incubation and CC vaccine mainly induced a cellular response. Despite these differences, all three approaches offered a prophylactic protection against tumor development in the murine mammary carcinoma model. In summary, it can be concluded that whole CC vaccines based on immunogenically killed CCs may not necessarily require association with DCs to elicit a protective anticancer immune response. If this finding can be endorsed in other cancer models, the manufacture of CC vaccines would greatly benefit from this new insight, as production of DC-based vaccines is laborious, time-consuming and expensive.
authors.affiliation: Laboratory of Gene Therapy, Department of Nutrition, Genetics and Ethology; Faculty of Veterinary Medicine; Ghent University ; Merelbeke, Belgium.
authors.forename: Laetitia
authors.initials: L
authors.lastname: Cicchelero
authors.affiliation: Laboratory of Gene Therapy, Department of Nutrition, Genetics and Ethology; Faculty of Veterinary Medicine; Ghent University ; Merelbeke, Belgium.
authors.forename: Sofie
authors.initials: S
authors.lastname: Denies
authors.affiliation: Laboratory of Immunology, Department of Virology, Parasitology and Immunology; Faculty of Veterinary Medicine; Ghent University , Merelbeke, Belgium.
authors.forename: Bert
authors.initials: B
authors.lastname: Devriendt
authors.affiliation: Small Animal Hospital, Department of Medicine and Clinical Biology of Small Animals; Faculty of Veterinary Medicine; Ghent University , Merelbeke, Belgium.
authors.forename: Hilde
authors.initials: H
authors.lastname: de Rooster
authors.affiliation: Laboratory of Gene Therapy, Department of Nutrition, Genetics and Ethology; Faculty of Veterinary Medicine; Ghent University ; Merelbeke, Belgium.
authors.forename: Niek N
authors.initials: NN
authors.lastname: Sanders
keywords: co-incubation; fusion; immunogenic cell death; whole cancer cell vaccine