title: Plasmonic Surfaces for Cell Growth and Retrieval Triggered by Near-Infrared Light.
abstract: Methods for efficient detachment of cells avoiding damage are required in tissue engineering and regenerative medicine. We introduce a bottom-up approach to build plasmonic substrates using micellar block copolymer nanolithography to generate a 2D array of Au seeds, followed by chemical growth leading to anisotropic nanoparticles. The resulting plasmonic substrates show a broad plasmon band covering a wide part of the visible and near-infrared (NIR) spectral ranges. Both human and murine cells were successfully grown on the substrates. A simple functionalization step of the plasmonic substrates with the cyclic arginylglycylaspartic acid (c-RGD) peptide allowed us to tune the morphology of integrin-rich human umbilical vein endothelial cells (HUVEC). Subsequent irradiation with a NIR laser led to highly efficient detachment of the cells with cell viability confirmed using the MTT assay. We thus propose the use of such plasmonic substrates for cell growth and controlled detachment using remote near-IR irradiation, as a general method for cell culture in biomedical applications.
authors.affiliation: CIC biomaGUNE, Paseo de Miramón 182, 20009, Donostia-San Sebastián, Spain. jjginer@cicbiomagune.es.|Biomedical Research Networking Center in Bioengineering, Biomaterials, and Nanomedicine (CIBER-BBN), Paseo de Miramón 182, 20009, Donostia-San Sebastián, Spain. jjginer@cicbiomagune.es.
authors.forename: Juan J
authors.initials: JJ
authors.lastname: Giner-Casares
authors.affiliation: CIC biomaGUNE, Paseo de Miramón 182, 20009, Donostia-San Sebastián, Spain.|Biomedical Research Networking Center in Bioengineering, Biomaterials, and Nanomedicine (CIBER-BBN), Paseo de Miramón 182, 20009, Donostia-San Sebastián, Spain.
authors.forename: Malou
authors.initials: M
authors.lastname: Henriksen-Lacey
authors.affiliation: CIC biomaGUNE, Paseo de Miramón 182, 20009, Donostia-San Sebastián, Spain.|Biomedical Research Networking Center in Bioengineering, Biomaterials, and Nanomedicine (CIBER-BBN), Paseo de Miramón 182, 20009, Donostia-San Sebastián, Spain.
authors.forename: Isabel
authors.initials: I
authors.lastname: García
authors.affiliation: CIC biomaGUNE, Paseo de Miramón 182, 20009, Donostia-San Sebastián, Spain. llizmarzan@cicbiomagune.es.|Biomedical Research Networking Center in Bioengineering, Biomaterials, and Nanomedicine (CIBER-BBN), Paseo de Miramón 182, 20009, Donostia-San Sebastián, Spain. llizmarzan@cicbiomagune.es.|Ikerbasque, Basque Foundation for Science, 48013, Bilbao, Spain. llizmarzan@cicbiomagune.es.
authors.forename: Luis M
authors.initials: LM
authors.lastname: Liz-Marzán
mesh_terms: D016475:3T3 Cells; D000818:Animals; D002448:Cell Adhesion; D002455:Cell Division; D006367:HeLa Cells; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D007259:Infrared Rays; D051379:Mice
keywords: cell cultures; cell harvesting; gold nanoparticles; plasmonics; tissue engineering