title: Role of cell wall deconstructing enzymes in the proanthocyanidin-cell wall adsorption-desorption phenomena.
abstract: The transference of proanthocyanidins from grapes to wine is quite low. This could be due, among other causes, to proanthocyanidins being bound to grape cell wall polysaccharides, which are present in high concentrations in the must. Therefore, the effective extraction of proanthocyanidins from grapes will depend on the ability to disrupt these associations, and, in this respect, enzymes that degrade these polysaccharides could play an important role. The main objective of this work was to test the behavior of proanthocyanidin-cell wall interactions when commercial maceration enzymes are present in the solution. The results showed that cell wall polysaccharides adsorbed a high amount of proanthocyanidins and only a limited quantity of proanthocyanidins could be desorbed from the cell walls after washing with a model solution. The presence of enzymes in the solution reduced the proanthocyanidin-cell wall interaction, probably through the elimination of pectins from the cell wall network.
authors.affiliation: Universidad de las Americas Puebla, Departamento de Ciencias de la Salud, Sta. Catarina Mártir, 72810 Cholula, Puebla, Mexico.
authors.forename: Liliana del Rocío
authors.initials: Ldel R
authors.lastname: Castro-López
authors.affiliation: Universidad de Murcia, Departamento de Tecnología de Alimentos, Nutrición y Bromatología, Facultad de Veterinaria, Campus de Espinardo, 30071 Murcia, Spain. Electronic address: encarna.gomez@um.es.
authors.forename: Encarna
authors.initials: E
authors.lastname: Gómez-Plaza
authors.affiliation: Universidad de las Americas Puebla, Departamento de Ciencias de la Salud, Sta. Catarina Mártir, 72810 Cholula, Puebla, Mexico.
authors.forename: Ana
authors.initials: A
authors.lastname: Ortega-Regules
authors.affiliation: Universidad de las Americas Puebla, Departamento de Ciencias de la Salud, Sta. Catarina Mártir, 72810 Cholula, Puebla, Mexico.
authors.forename: Daniel
authors.initials: D
authors.lastname: Lozada
authors.affiliation: Universidad de Murcia, Departamento de Tecnología de Alimentos, Nutrición y Bromatología, Facultad de Veterinaria, Campus de Espinardo, 30071 Murcia, Spain.
authors.forename: Ana Belén
authors.initials: AB
authors.lastname: Bautista-Ortín
mesh_terms: D000327:Adsorption; D002473:Cell Wall / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry; D044945:Proanthocyanidins / Q000737:chemistry*; D027843:Vitis / Q000737:chemistry*; D014920:Wine / Q000032:analysis
chemical_list: D011134:Polysaccharides; D044945:Proanthocyanidins; C013221:proanthocyanidin
keywords: Cell wall; Phloroglucinolysis; Polysaccharides; Proanthocyanidin; Size exclusion chromatography; Tannins