title: Neuropeptides in the cerebral ganglia of the mud crab, Scylla paramamosain: transcriptomic analysis and expression profiles during vitellogenesis.
abstract: Neuropeptides play a critical role in regulating animal reproduction. In vertebrates, GnRH, GnIH and kisspeptin are the key neuropeptide hormones of the reproductive axis, however, the reproductive axis for invertebrates is vague. Knowledge on ovarian development of the mud crab, Scylla paramamosain, is critical for aquaculture and resources management of the commercially important species. This study employed Illumina sequencing, reverse transcription-polymerase chain reaction and quantitative real-time PCR techniques to identify neuropeptides that may be involved in ovarian development of S. paramamosain. A total of 32 neuropeptide transcripts from two dozen neuropeptide families, 100 distinct mature peptides were predicted from the transcriptome data of female S. paramamosain cerebral ganglia. Among them, two families, i.e. GSEFLamide and WXXXRamide, were first identified from the cerebral ganglia of crustaceans. Of these neuropeptides, 21 transcripts of interest were selected for further confirmation and all of them were detected in the cerebral ganglia, as well as in other nervous tissues and the ovary. Most of them also had differential expression in the cerebral ganglia during various vitellogenic stages, suggesting their likely involvement in regulating vitellogenesis and ovarian maturation. Overall, these findings provide an important basis for subsequent studies on peptide function in reproduction of S. paramamosain.
authors.affiliation: College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China.
authors.forename: Chenchang
authors.initials: C
authors.lastname: Bao
authors.affiliation: State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361102, China.
authors.forename: Yanan
authors.initials: Y
authors.lastname: Yang
authors.affiliation: College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China.
authors.forename: Huiyang
authors.initials: H
authors.lastname: Huang
authors.affiliation: College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China.|Collaborative Innovation Center for Development and Utilization of Marine Biological Resources, Xiamen 361102, China.
authors.forename: Haihui
authors.initials: H
authors.lastname: Ye
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D060829:Arthropod Proteins / Q000235:genetics* / Q000378:metabolism; D003386:Brachyura / Q000235:genetics* / Q000254:growth & development; D001921:Brain / Q000254:growth & development / Q000378:metabolism; D005260:Female; D017952:Ganglia, Invertebrate / Q000254:growth & development / Q000378:metabolism*; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D058977:Molecular Sequence Annotation; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000235:genetics* / Q000378:metabolism; D009928:Organ Specificity; D010053:Ovary / Q000254:growth & development / Q000378:metabolism; D012098:Reproduction / Q000235:genetics; D016415:Sequence Alignment; D059467:Transcriptome; D014818:Vitellogenesis / Q000235:genetics*
chemical_list: D060829:Arthropod Proteins; D009479:Neuropeptides