title: LC-MS-Based Metabolomic Investigation of Chemopreventive Phytochemical-Elicited Metabolic Events.
abstract: Phytochemicals are under intensive investigation for their potential use as chemopreventive agents in blocking or suppressing carcinogenesis. Metabolic interactions between phytochemical and biological system play an important role in determining the efficacy and toxicity of chemopreventive phytochemicals. However, complexities of phytochemical biotransformation and intermediary metabolism pose challenges for studying phytochemical-elicited metabolic events. Metabolomics has become a highly effective technical platform to detect subtle changes in a complex metabolic system. Here, using green tea polyphenols as an example, we describe a workflow of LC-MS-based metabolomics study, covering the procedures and techniques in sample collection, preparation, LC-MS analysis, data analysis, and interpretation.
authors.affiliation: Department of Food Science and Nutrition, University of Minnesota, 1334 Eckles Ave, St. Paul, MN, 55108, USA.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Food Science and Nutrition, University of Minnesota, 1334 Eckles Ave, St. Paul, MN, 55108, USA.
authors.forename: Dan
authors.initials: D
authors.lastname: Yao
authors.affiliation: Department of Food Science and Nutrition, University of Minnesota, 1334 Eckles Ave, St. Paul, MN, 55108, USA. chichen@umn.edu.
authors.forename: Chi
authors.initials: C
authors.lastname: Chen
mesh_terms: D053000:Analytic Sample Preparation Methods; D000818:Animals; D018890:Chemoprevention; D002853:Chromatography, Liquid / Q000379:methods*; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D055432:Metabolomics / Q000379:methods*; D051379:Mice; D008810:Mice, Inbred C57BL; D064209:Phytochemicals / Q000494:pharmacology*; D059808:Polyphenols / Q000494:pharmacology; D013662:Tea / Q000737:chemistry
chemical_list: D064209:Phytochemicals; D059808:Polyphenols; D013662:Tea
keywords: Chemoprevention; LC-MS; Metabolism; Metabolomics; Phytochemical