title: A novel gemycircularvirus in an unexplained case of child encephalitis.
abstract: BACKGROUND
  Recently, a diverse group of viruses with circular, replication initiator protein(Rep) encoding, single stranded DNA (CRESS-DNA) genomes, were discovered from wide range of eukaryotic organisms ranging from mammals to fungi. Gemycircularvirus belongs to a distinct group of CRESS-DNA genomes and is classified under the genus name of Gemycircularvirus.
  
  
  RESULTS
  Here, a novel gemycircularvirus named GeTz1 from cerebrospinal fluid sample of a child with unexplainable encephalitis was characterized. The novel gemycircularvirus encodes two major proteins, including a capsid protein (Cap) and a replication-associated protein (Rep). Phylogenetic analysis based on the amino acid sequence of Rep indicated that GeTz1 clusters with one gemycircularvirus discovered from bird (KF371633), sharing 46.6 % amino acid sequence identity with each other.
  
  
  CONCLUSIONS
  A novel gemycircularvirus was discovered from cerebrospinal fluid sample of a child with unexplainable encephalitis. Further studies, such as testing human sera for specific antibodies, should be performed to investigate whether gemycircularvirus infects human and is associated with encephalitis.
authors.affiliation: Department of Laboratory Medicine, Jiangsu Taizhou People's Hospital, Taizhou, Jiangsu, 225300, China. clzhoutaizhou@126.com.
authors.forename: Chenglin
authors.initials: C
authors.lastname: Zhou
authors.affiliation: Department of Laboratory Medicine, the First People's Hospital of Suqian, Suqian, Jiangsu, 223800, China. songyyzj@163.com.
authors.forename: Shibing
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Jiangsu Taizhou People's Hospital, Taizhou, Jiangsu, 225300, China. 380208024@qq.com.
authors.forename: Qin
authors.initials: Q
authors.lastname: Gong
authors.affiliation: Department of Laboratory Medicine, the Second People's Hospital of Wuxi, Wuxi, Jiangsu, 214002, China. aiminhaowuxi@yeah.net.
authors.forename: Aimin
authors.initials: A
authors.lastname: Hao
mesh_terms: D036022:Capsid Proteins / Q000235:genetics; D002555:Cerebrospinal Fluid / Q000821:virology; D002675:Child, Preschool; D016000:Cluster Analysis; D004265:DNA Helicases / Q000235:genetics; D004266:DNA Virus Infections / Q000175:diagnosis* / Q000473:pathology / Q000821:virology*; D004267:DNA Viruses / Q000235:genetics / Q000302:isolation & purification*; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D018792:Encephalitis, Viral / Q000175:diagnosis* / Q000473:pathology / Q000821:virology*; D006801:Humans; D007223:Infant; D008969:Molecular Sequence Data; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D015534:Trans-Activators / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics
chemical_list: D036022:Capsid Proteins; D004279:DNA, Viral; D015534:Trans-Activators; D014764:Viral Proteins; C045264:replication initiator protein; D004265:DNA Helicases