title: Quantitative Organic Acids in Urine by Two Dimensional Gas Chromatography-Time of Flight Mass Spectrometry (GCxGC-TOFMS).
abstract: Seventy-six organic acids in urine specimens are determined with quantitative two dimensional Gas Chromatography-Time of Flight Mass Spectrometry (GCxGC-TOFMS). The specimen is treated with urease to remove urea then derivatized to form pentafluorobenzyl oximes (PFBO) of oxoacids. The sample is then treated with ethyl alcohol to precipitate proteins and centrifuged. After drying the supernatant, the organic acids are derivatized to form volatile trimethylsilyl (TMS) derivatives for separation by capillary two dimensional Gas Chromatography (GCxGC) with temperature programming and modulation. Detection is by Time of Flight Mass Spectrometry (TOFMS) with identification of the organic acids by their mass spectra. Organic acids are quantitated by peak areas of reconstructed ion chromatograms with internal standards and calibration curves. Organic acids are quantified to determine abnormal patterns for the diagnosis of more than 100 inherited disorders of organic acid metabolism. Characteristic abnormal metabolites are quantified to monitor dietary and other modes of treatment for patients who are diagnosed with specific organic acid disorders.
authors.affiliation: Department of Mass Spectrometry, Center of Metabolomics, Baylor Research Institute, 3812 Elm Street, Dallas, TX, USA. larrys@baylorhealth.edu.
authors.forename: Lawrence
authors.initials: L
authors.lastname: Sweetman
authors.affiliation: Department of Mass Spectrometry, Center of Metabolomics, Baylor Research Institute, 3812 Elm Street, Dallas, TX, USA.
authors.forename: Paula
authors.initials: P
authors.lastname: Ashcraft
authors.affiliation: Department of Mass Spectrometry, Center of Metabolomics, Baylor Research Institute, 3812 Elm Street, Dallas, TX, USA.
authors.forename: Jeanna
authors.initials: J
authors.lastname: Bennett-Firmin
mesh_terms: D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D009930:Organic Chemicals / Q000652:urine*; D013223:Statistics as Topic; D016482:Urinalysis / Q000379:methods*
chemical_list: D009930:Organic Chemicals
keywords: Mass spectrometry; Trimethylsilyl derivatives (TMS); Urine organic acid s