title: [The duodenal microbiota composition in children with active coeliac disease is influenced by the degree of enteropathy].
abstract: OBJECTIVE
  To establish whether the duodenal mucosa microbiota of children with active coeliac disease (CD) and healthy controls (HC) differ in composition and biodiversity.
  
  
  METHODS
  Samples of duodenal biopsies in 11 CD patients were obtained at diagnosis, and in 6 HC who were investigated for functional intestinal disorders of non-CD origin. Total duodenal microbiota and the belonging to the genus Lactobacillus using PCR-denaturing gradient gel electrophoresis (DGGE) were analysed. The banding patterns obtained in the resulting gels were analysed to determine the differences between the microbiota of CD patients and HC (FPQuest 4.5) while environmental indexes (richness, diversity and habitability) were calculated with the Past version 2.17 program.
  
  
  RESULTS
  The intestinal microbiota of patients with Marsh 3c lesion showed similarity of 98% and differs from other CD patients with other type of histologic lesion as Marsh3a, Marsh3b and Marsh2. The main differences were obtained in ecological indexes belonging to the genus Lactobacillus, with significant richness, diversity and habitability reduction in CD patients. In CD bands were categorized primarily with Streptococcus, Bacteroides and E.coli species. In HC the predominant bands were Bifidobacterium, Lactobacillus and Acinetobacter, though the Streptococcus and Bacteroides were lower.
  
  
  CONCLUSIONS
  The celiac patients with major histological affectation presented a similar microbiota duodenal. The ecological indexes applied to the genus Lactobacillus were significantly reduced in CD.
authors.affiliation: Unidad de Gastroenterología y Nutrición Infantil, Hospital Materno Infantil, Málaga, España.
authors.forename: F
authors.initials: F
authors.lastname: Girón Fernández-Crehuet
authors.affiliation: Departamento de Microbiología, Facultad de Ciencias, Málaga, España.
authors.forename: S
authors.initials: S
authors.lastname: Tapia-Paniagua
authors.affiliation: Departamento de Microbiología, Facultad de Ciencias, Málaga, España.
authors.forename: M A
authors.initials: MA
authors.lastname: Moriñigo Gutiérrez
authors.affiliation: Unidad de Gastroenterología y Nutrición Infantil, Hospital Materno Infantil, Málaga, España; Instituto de Investigación Biomédica de Málaga (IBIMA), Málaga, España.
authors.forename: V M
authors.initials: VM
authors.lastname: Navas-López
authors.affiliation: Unidad de Gastroenterología y Nutrición Infantil, Hospital Materno Infantil, Málaga, España.
authors.forename: M
authors.initials: M
authors.lastname: Juliana Serrano
authors.affiliation: Unidad de Gastroenterología y Nutrición Infantil, Hospital Materno Infantil, Málaga, España; Instituto de Investigación Biomédica de Málaga (IBIMA), Málaga, España.
authors.forename: J
authors.initials: J
authors.lastname: Blasco-Alonso
authors.affiliation: Unidad de Gastroenterología y Nutrición Infantil, Hospital Materno Infantil, Málaga, España; Instituto de Investigación Biomédica de Málaga (IBIMA), Málaga, España. Electronic address: carlos.sierra.sspa@juntadeandalucia.es.
authors.forename: C
authors.initials: C
authors.lastname: Sierra Salinas
mesh_terms: D044822:Biodiversity; D016022:Case-Control Studies; D002446:Celiac Disease / Q000382:microbiology*; D002648:Child; D004386:Duodenum / Q000382:microbiology*; D006801:Humans; D064307:Microbiota; D016133:Polymerase Chain Reaction
keywords: Coeliac disease; Enfermedad celíaca; Lactobacillus; MARSH; Microbiota