title: Derivation of Rabbit Embryonic Stem Cells from Vitrified-Thawed Embryos.
abstract: The rabbit is a useful animal model for regenerative medicine. We previously developed pluripotent rabbit embryonic stem cell (rbESC) lines using fresh embryos. We also successfully cryopreserved rabbit embryos by vitrification. In the present work, we combined these two technologies to derive rbESCs using vitrified-thawed (V/T) embryos. We demonstrate that V/T blastocysts (BLs) can be used to derive pluripotent rbESCs with efficiencies comparable to those using fresh BLs. These ESCs are undistinguishable from the ones derived from fresh embryos. We tested the developmental capacity of rbESCs derived from V/T embryos by BL injection experiments and produced chimeric kits. Our work adds cryopreservation to the toolbox of rabbit stem cell research and applications and will greatly expand the available research materials for regenerative medicine in a clinically relevant animal model.
authors.affiliation: 1 Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Sciences, Nanjing Normal University , Nanjing 210046, China .|2 Renova Life, Inc. , College Park, Maryland 20742.|5 These authors contributed equally to this work.
authors.forename: Fuliang
authors.initials: F
authors.lastname: Du
authors.affiliation: 2 Renova Life, Inc. , College Park, Maryland 20742.|5 These authors contributed equally to this work.
authors.forename: Chien-Hong
authors.initials: CH
authors.lastname: Chen
authors.affiliation: 1 Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Sciences, Nanjing Normal University , Nanjing 210046, China .
authors.forename: Yi
authors.initials: Y
authors.lastname: Li
authors.affiliation: 1 Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Sciences, Nanjing Normal University , Nanjing 210046, China .
authors.forename: Yeshu
authors.initials: Y
authors.lastname: Hu
authors.affiliation: 1 Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Sciences, Nanjing Normal University , Nanjing 210046, China .
authors.forename: Li-You
authors.initials: LY
authors.lastname: An
authors.affiliation: 3 Lannuo Biotechnologies Wuxi, Inc. , Wuxi, Jiangsu 214174, China .
authors.forename: Lan
authors.initials: L
authors.lastname: Yang
authors.affiliation: 4 Center for Advanced Models for Translational Sciences and Therapeutics, University of Michigan Medical Center , Ann Arbor, MI, 48109.
authors.forename: Jifeng
authors.initials: J
authors.lastname: Zhang
authors.affiliation: 4 Center for Advanced Models for Translational Sciences and Therapeutics, University of Michigan Medical Center , Ann Arbor, MI, 48109.
authors.forename: Y Eugene
authors.initials: YE
authors.lastname: Chen
authors.affiliation: 2 Renova Life, Inc. , College Park, Maryland 20742.|4 Center for Advanced Models for Translational Sciences and Therapeutics, University of Michigan Medical Center , Ann Arbor, MI, 48109.
authors.forename: Jie
authors.initials: J
authors.lastname: Xu
mesh_terms: D000577:Amides / Q000737:chemistry; D000818:Animals; D001755:Blastocyst / Q000166:cytology*; D002454:Cell Differentiation; D002460:Cell Line; D015925:Cryopreservation / Q000379:methods*; D004624:Embryo Transfer; D053595:Embryonic Stem Cells / Q000166:cytology*; D005260:Female; D007150:Immunohistochemistry; D007621:Karyotyping; D051379:Mice; D011725:Pyridines / Q000737:chemistry; D011817:Rabbits; D012038:Regeneration; D013696:Temperature; D058989:Vitrification
chemical_list: D000577:Amides; D011725:Pyridines; C108830:Y 27632