title: Monocyte Stabilin-1 Suppresses the Activation of Th1 Lymphocytes.
abstract: In this study, we analyzed the putative functions of stabilin-1 in blood monocytes. Microarray analysis revealed downregulation of several proinflammatory genes in the stabilin-1(high) monocytes when compared with stabilin-1(low) monocytes. When cocultured with stabilin-1(high) monocytes, IFN-γ synthesis by T cells was diminished in Ag-recall assays. Knockdown of stabilin-1 in monocytes increased the synthesis of several proinflammatory molecules, including TNF-α, and supported high IFN-γ and low IL-4 and IL-5 production by T cells in Ag-specific stimulation assays. Anti-stabilin-1 Ab treatment also led to increased IFN-γ synthesis in the recall assays. In clinical settings, the expression of stabilin-1 was diminished on blood monocytes and tissue macrophages under proinflammatory conditions. These data define stabilin-1 as a new immunosuppressive molecule and suggest that stabilin-1(high) monocytes may dampen proinflammatory reactions in vivo.
authors.affiliation: MediCity Research Laboratory, University of Turku, Turku 20520, Finland; Turku Doctoral Programme of Molecular Medicine, University of Turku, Turku 20520, Finland; Turku Doctoral Programme of Biomedical Sciences, University of Turku, Turku 20520, Finland;
authors.forename: Senthil
authors.identifier: http://orcid.org/0000-0001-7435-5398
authors.initials: S
authors.lastname: Palani
authors.affiliation: MediCity Research Laboratory, University of Turku, Turku 20520, Finland; Department of Medical Biochemistry and Genetics, University of Turku, Turku 20520, Finland;
authors.forename: Kati
authors.initials: K
authors.lastname: Elima
authors.affiliation: Department of Obstetrics and Gynecology, University of Turku, Turku 20520, Finland; and.
authors.forename: Eeva
authors.initials: E
authors.lastname: Ekholm
authors.affiliation: MediCity Research Laboratory, University of Turku, Turku 20520, Finland; Department of Medical Microbiology and Immunology, University of Turku, Turku 20520, Finland.
authors.forename: Sirpa
authors.initials: S
authors.lastname: Jalkanen
authors.affiliation: MediCity Research Laboratory, University of Turku, Turku 20520, Finland; Department of Medical Microbiology and Immunology, University of Turku, Turku 20520, Finland marko.salmi@utu.fi.
authors.forename: Marko
authors.initials: M
authors.lastname: Salmi
mesh_terms: D001483:Base Sequence; D015816:Cell Adhesion Molecules, Neuronal / Q000235:genetics*; D002478:Cells, Cultured; D015536:Down-Regulation; D006801:Humans; D007249:Inflammation / Q000276:immunology*; D007371:Interferon-gamma / Q000096:biosynthesis; D015847:Interleukin-4 / Q000096:biosynthesis; D015848:Interleukin-5 / Q000096:biosynthesis; D008213:Lymphocyte Activation / Q000276:immunology*; D008264:Macrophages / Q000276:immunology; D009000:Monocytes / Q000276:immunology*; D020411:Oligonucleotide Array Sequence Analysis; D034622:RNA Interference; D034741:RNA, Small Interfering; D016201:Receptors, Lymphocyte Homing / Q000235:genetics*; D017423:Sequence Analysis, RNA; D018417:Th1 Cells / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
chemical_list: D015816:Cell Adhesion Molecules, Neuronal; C508598:IL4 protein, human; C508599:IL5 protein, human; D015848:Interleukin-5; D034741:RNA, Small Interfering; D016201:Receptors, Lymphocyte Homing; C449926:STAB1 protein, human; D014409:Tumor Necrosis Factor-alpha; D015847:Interleukin-4; D007371:Interferon-gamma