title: Production of pyruvate from mannitol by mannitol-assimilating pyruvate decarboxylase-negative Saccharomyces cerevisiae.
abstract: Mannitol is contained in brown macroalgae up to 33% (w/w, dry weight), and thus is a promising carbon source for white biotechnology. However, Saccharomyces cerevisiae, a key cell factory, is generally regarded to be unable to assimilate mannitol for growth. We have recently succeeded in producing S. cerevisiae that can assimilate mannitol through spontaneous mutations of Tup1-Cyc8, each of which constitutes a general corepressor complex. In this study, we demonstrate production of pyruvate from mannitol using this mannitol-assimilating S. cerevisiae through deletions of all 3 pyruvate decarboxylase genes. The resultant mannitol-assimilating pyruvate decarboxylase-negative strain produced 0.86 g/L pyruvate without use of acetate after cultivation for 4 days, with an overall yield of 0.77 g of pyruvate per g of mannitol (the theoretical yield was 79%). Although acetate was not needed for growth of this strain in mannitol-containing medium, addition of acetate had a significant beneficial effect on production of pyruvate. This is the first report of production of a valuable compound (other than ethanol) from mannitol using S. cerevisiae, and is an initial platform from which the productivity of pyruvate from mannitol can be improved.
authors.affiliation: a Laboratory of Basic and Applied Molecular Biotechnology; Division of Food Science and Biotechnology; Graduate School of Agriculture; Kyoto University ; Uji , Kyoto , Japan.
authors.forename: Shiori
authors.initials: S
authors.lastname: Yoshida
authors.affiliation: a Laboratory of Basic and Applied Molecular Biotechnology; Division of Food Science and Biotechnology; Graduate School of Agriculture; Kyoto University ; Uji , Kyoto , Japan.
authors.forename: Hideki
authors.initials: H
authors.lastname: Tanaka
authors.affiliation: a Laboratory of Basic and Applied Molecular Biotechnology; Division of Food Science and Biotechnology; Graduate School of Agriculture; Kyoto University ; Uji , Kyoto , Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Hirayama
authors.affiliation: a Laboratory of Basic and Applied Molecular Biotechnology; Division of Food Science and Biotechnology; Graduate School of Agriculture; Kyoto University ; Uji , Kyoto , Japan.|b Faculty of Science and Engineering; Department of Life Science ; Setsunan University ; Neyagawa , Osaka , Japan.
authors.forename: Kousaku
authors.initials: K
authors.lastname: Murata
authors.affiliation: a Laboratory of Basic and Applied Molecular Biotechnology; Division of Food Science and Biotechnology; Graduate School of Agriculture; Kyoto University ; Uji , Kyoto , Japan.
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Kawai
mesh_terms: D019342:Acetic Acid / Q000378:metabolism; D017353:Gene Deletion; D005800:Genes, Fungal; D007700:Kinetics; D008353:Mannitol / Q000378:metabolism*; D060847:Metabolic Engineering / Q000379:methods*; D011767:Pyruvate Decarboxylase / Q000172:deficiency / Q000235:genetics / Q000378:metabolism; D019289:Pyruvic Acid / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D029701:Saccharomyces cerevisiae Proteins; D008353:Mannitol; D019289:Pyruvic Acid; D011767:Pyruvate Decarboxylase; D019342:Acetic Acid
keywords: Saccharomyces cerevisiae; brown macroalgae; mannitol; pyruvate; pyruvate decarboxylase