title: Hepcidin determination in dried blood by microfluidic LC-MS/MS: comparison of DBS and volumetric absorptive microsampling for matrix effect and recovery.
abstract: BACKGROUND
  Dried blood analysis experiences a growing interest due to practical, ethical and financial advantages compared with classical wet plasma or serum analysis. Besides classical DBS, new alternatives are commercialized as volumetric absorptive microsampling (VAMS) that are expected to overcome hematocrit influence.
  
  
  RESULTS
  The feasibility of hepcidin (a peptide hormone) extraction and determination from DBS and VAMS blood sampling was investigated. Experimental design was used to determine the optimal extraction conditions. Matrix effect and extraction recovery were studied and a special attention was paid to phospholipid removal.
  
  
  CONCLUSIONS
  The data suggest that the combination of VAMS and phospholipid removal plates provides low matrix effect and high sensitivity, and constitutes an easy and promising protocol for hepcidin analysis.
authors.affiliation: Laboratory for the Analysis of Medicines, Department of Pharmacy, CIRM, University of Liege, Belgium.
authors.forename: Virginie
authors.initials: V
authors.lastname: Houbart
authors.affiliation: Department of Clinical Chemistry, CHU, University of Liege, Belgium.
authors.forename: Gaël
authors.initials: G
authors.lastname: Cobraiville
authors.affiliation: Laboratory for the Analysis of Medicines, Department of Pharmacy, CIRM, University of Liege, Belgium.
authors.forename: Anne-Catherine
authors.initials: AC
authors.lastname: Servais
authors.affiliation: Laboratory for the Analysis of Medicines, Department of Pharmacy, CIRM, University of Liege, Belgium.
authors.forename: Aurore
authors.initials: A
authors.lastname: Napp
authors.affiliation: Department of Clinical Chemistry, CHU, University of Liege, Belgium.
authors.forename: Marie-Paule
authors.initials: MP
authors.lastname: Merville
authors.affiliation: Laboratory for the Analysis of Medicines, Department of Pharmacy, CIRM, University of Liege, Belgium.
authors.forename: Marianne
authors.initials: M
authors.lastname: Fillet
mesh_terms: D000890:Anti-Infective Agents / Q000032:analysis; D001800:Blood Specimen Collection / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods*; D059788:Dried Blood Spot Testing / Q000379:methods*; D006400:Hematocrit; D064451:Hepcidins / Q000032:analysis*; D006801:Humans; D044085:Microfluidics / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000890:Anti-Infective Agents; D064451:Hepcidins