title: Evaluation of multidrug resistance-1 gene C>T polymorphism frequency in patients with asthma.
abstract: OBJECTIVE
  Asthma is a chronic inflammatory lung disease characterized by bronchial hyperresponsiveness and airflow obstruction. Genetic and oxidative stress factors, in addition to pulmonary and systemic inflammatory processes, play a pivotal role in the pathogenesis of asthma. The products of the multidrug resistance-1 gene protect lung tissue from oxidative stress. Here, we aimed to evaluate the association between the multidrug resistance-1 gene C>T polymorphism and asthma with regard to oxidative stress-related parameters of asthmatic patients.
  
  
  METHODS
  Forty-five patients with asthma and 27 healthy age-matched controls were included in this study. Blood samples were collected in tubes with ethylenediaminetetraacetic acid. DNA was extracted from the blood samples. The multidrug resistance-1 gene polymorphism was detected by polymerase chain reaction and a subsequent enzyme digestion technique. The serum levels of total oxidant status and total antioxidant status were determined by the colorimetric measurement method.
  
  
  RESULTS
  The heterozygous polymorphic genotype was the most frequent in both groups. A significant difference in the multidrug resistance-1 genotype frequencies between groups indicated an association of asthma with the TT genotype. A significant difference between groups was found for wild type homozygous participants and carriers of polymorphic allele participants. The frequency of the T allele was significantly higher in asthmatic patients. The increase in the oxidative stress index parameter was significant in the asthma group compared with the control group.
  
  
  CONCLUSIONS
  The multidrug resistance-1 gene C/T polymorphism may be an underlying genetic risk factor for the development of asthma via oxidant-antioxidant imbalance, leading to increased oxidative stress.
authors.affiliation: Department of Chest Diseases, Faculty of Medicine, Dumlupınar University, Kütahya, Turkey.
authors.forename: Ümran
authors.initials: Ü
authors.lastname: Toru
authors.affiliation: Department of Physiology, Faculty of Medicine, Dumlupınar University, Kütahya, Turkey.
authors.forename: Ceylan
authors.initials: C
authors.lastname: Ayada
authors.affiliation: Department of Physiology, Faculty of Medicine, Dumlupınar University, Kütahya, Turkey.
authors.forename: Osman
authors.initials: O
authors.lastname: Genç
authors.affiliation: Department of Chest Diseases, Faculty of Medicine, Abant İzzet Baysal University, Bolu, Turkey.
authors.forename: Zehra
authors.initials: Z
authors.lastname: Yaşar
authors.affiliation: Department of Medical Biology, Faculty of Medicine, Dumlupınar University, Kütahya, Turkey.
authors.forename: Server
authors.initials: S
authors.lastname: Şahin
authors.affiliation: Department of Medical Biology, Faculty of Medicine, Karabük University, Karabük, Turkey.
authors.forename: Emre
authors.initials: E
authors.lastname: Taşkın
authors.affiliation: Süreyyapaşa Chest Diseases and Thoracic Surgery Training and Research Hospital, Department of Allergy and Immunology, İstanbul, Turkey.
authors.forename: İsmet
authors.initials: İ
authors.lastname: Bulut
authors.affiliation: Aydın General Secretary of the Union of Public Hospitals, Aydın, Turkey.
authors.forename: Murat
authors.initials: M
authors.lastname: Acat
mesh_terms: D018435:ATP Binding Cassette Transporter, Subfamily B / Q000235:genetics; D000328:Adult; D001249:Asthma / Q000235:genetics*; D016022:Case-Control Studies; D005260:Female; D019450:Genes, MDR; D006579:Heterozygote; D006801:Humans; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D018709:Statistics, Nonparametric
chemical_list: C513055:ABCB1 protein, human; D018435:ATP Binding Cassette Transporter, Subfamily B