title: Enhancement of energy production by black ginger extract containing polymethoxy flavonoids in myocytes through improving glucose, lactic acid and lipid metabolism.
abstract: Enhancement of muscular energy production is thought to improve locomotive functions and prevent metabolic syndromes including diabetes and lipidemia. Black ginger (Kaempferia parviflora) has been cultivated for traditional medicine in Thailand. Recent studies have shown that black ginger extract (KPE) activated brown adipocytes and lipolysis in white adipose tissue, which may cure obesity-related dysfunction of lipid metabolism. However, the effect of KPE on glucose and lipid utilization in muscle cells has not been examined yet. Hence, we evaluated the effect of KPE and its constituents on energy metabolism in pre-differentiated (p) and differentiated (d) C2C12 myoblasts. KPE (0.1-10 μg/ml) was added to pC2C12 cells in the differentiation process for a week or used to treat dC2C12 cells for 24 h. After culturing, parameters of glucose and lipid metabolism and mitochondrial biogenesis were assessed. In terms of the results, KPE enhanced the uptake of 2-deoxyglucose and lactic acid as well as the mRNA expression of glucose transporter (GLUT) 4 and monocarboxylate transporter (MCT) 1 in both types of cells. The expression of peroxisome proliferator-activated receptor γ coactivator (PGC)-1α was enhanced in pC2C12 cells. In addition, KPE enhanced the production of ATP and mitochondrial biogenesis. Polymethoxy flavonoids in KPE including 5-hydroxy-7-methoxyflavone, 5-hydroxy-3,7,4'-trimethoxyflavone and 5,7-dimethoxyflavone enhanced the expression of GLUT4 and PGC-1α. Moreover, KPE and 5,7-dimethoxyflavone enhanced the phosphorylation of 5'AMP-activated protein kinase (AMPK). In conclusion, KPE and its polymethoxy flavonoids were found to enhance energy metabolism in myocytes. KPE may improve the dysfunction of muscle metabolism that leads to metabolic syndrome and locomotive dysfunction.
authors.affiliation: Research and Development Division, Oryza Oil and Fat Chemical Co., Ltd., 1 Numata, Kitagata-cho, Ichinomiya, Aichi, 493-8001, Japan.
authors.forename: Kazuya
authors.initials: K
authors.lastname: Toda
authors.affiliation: Research and Development Division, Oryza Oil and Fat Chemical Co., Ltd., 1 Numata, Kitagata-cho, Ichinomiya, Aichi, 493-8001, Japan.
authors.forename: Shogo
authors.initials: S
authors.lastname: Takeda
authors.affiliation: Research and Development Division, Oryza Oil and Fat Chemical Co., Ltd., 1 Numata, Kitagata-cho, Ichinomiya, Aichi, 493-8001, Japan.
authors.forename: Shoketsu
authors.initials: S
authors.lastname: Hitoe
authors.affiliation: Kyoto Pharmaceutical University, 1 Shichono-cho, Misasagi, Yamashina-Ku, Kyoto, 607-8412, Japan.
authors.forename: Seikou
authors.initials: S
authors.lastname: Nakamura
authors.affiliation: Kyoto Pharmaceutical University, 1 Shichono-cho, Misasagi, Yamashina-Ku, Kyoto, 607-8412, Japan.
authors.forename: Hisashi
authors.initials: H
authors.lastname: Matsuda
authors.affiliation: Research and Development Division, Oryza Oil and Fat Chemical Co., Ltd., 1 Numata, Kitagata-cho, Ichinomiya, Aichi, 493-8001, Japan. kaihatsu@mri.biglobe.ne.jp.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Shimoda
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D003847:Deoxyglucose / Q000378:metabolism; D004734:Energy Metabolism / Q000187:drug effects*; D005419:Flavonoids / Q000494:pharmacology*; D020939:Zingiber officinale; D005947:Glucose / Q000378:metabolism*; D051275:Glucose Transporter Type 4 / Q000378:metabolism; D019344:Lactic Acid / Q000378:metabolism*; D050356:Lipid Metabolism / Q000187:drug effects*; D008066:Lipolysis / Q000187:drug effects; D051379:Mice; D018482:Muscle, Skeletal / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D009765:Obesity / Q000150:complications / Q000378:metabolism; D047495:PPAR gamma / Q000378:metabolism; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use; D013785:Thailand; D014157:Transcription Factors / Q000378:metabolism; D030521:Zingiberaceae / Q000737:chemistry*
chemical_list: C574159:5-hydroxy-7-methoxyflavone; D005419:Flavonoids; D051275:Glucose Transporter Type 4; D047495:PPAR gamma; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; D010936:Plant Extracts; C485290:Ppargc1a protein, mouse; D014157:Transcription Factors; C060298:5,7-dimethoxyflavone; D019344:Lactic Acid; D003847:Deoxyglucose; D055372:AMP-Activated Protein Kinases; D005947:Glucose
keywords: 5′AMP-activated protein kinase; Glucose transporter 4; Kaempferia parviflora; Lactic acid; Myoblast; Peroxisome proliferator-activated receptor γ coactivator