title: Chemical composition of the essential oil from the leaves of Anaxagorea brevipes (Annonaceae) and evaluation of its bioactivity.
abstract: The essential oil obtained by hydrodistillation from leaves of Anaxagorea brevipes was analysed by gas chromatography fitted with a flame ionisation detector (GC-FID) and coupled to mass spectrometry (GC-MS). Thirty one components were identified, representing around 75.7% of total oil. The major components were β-eudesmol (13.16%), α-eudesmol (13.05%), γ-eudesmol (7.54%), guaiol (5.12%), caryophyllene oxide (4.18%) and β-bisabolene (4.10%). The essential oil showed antimicrobial activity against Gram-positive bacteria and yeast with the MIC values between 25.0 and 100 μg/mL. The highest antiproliferative activity was observed for the oil against MCF-7 (breast, TGI = 12.8 μg/mL), NCI-H460 (lung, TGI = 13.0 μg/mL) and PC-3 (prostate, TGI = 9.6 μg/mL) cell lines, while against no cancer cell line HaCat (keratinocyte) the TGI was 38.8 μg/mL. The oil exhibited a small antioxidant activity assessed through ORAC-FL assay (517 μmol TE/g). This is the first report regarding the chemical composition and bioactivity of A. brevipes essential oil.
authors.affiliation: a Departamento de Química , Universidade Federal do Amazonas , Manaus , Brazil.
authors.forename: Danielle Cardoso
authors.initials: DC
authors.lastname: de Alencar
authors.affiliation: a Departamento de Química , Universidade Federal do Amazonas , Manaus , Brazil.
authors.forename: Maria Lúcia Belém
authors.initials: ML
authors.lastname: Pinheiro
authors.affiliation: a Departamento de Química , Universidade Federal do Amazonas , Manaus , Brazil.
authors.forename: José Lamak da Silva
authors.initials: JL
authors.lastname: Pereira
authors.affiliation: b Divisão Farmacologia e Toxicologia , CPQBA, UNICAMP , Campinas , Brazil.
authors.forename: João Ernesto
authors.initials: JE
authors.lastname: de Carvalho
authors.affiliation: c Departamento de Farmácia , Universidade Federal do Paraná, Campus Jardim Botânico, Jardim Botânico , Curitiba , Brazil.
authors.forename: Francinete Ramos
authors.initials: FR
authors.lastname: Campos
authors.affiliation: d Departamento de Biologia Vegetal, PPG BTPB and PPG BV, Instituto de Biologia , Universidade Estadual de Campinas (UNICAMP) , Campinas , Brazil.
authors.forename: Alessandra Freitas
authors.initials: AF
authors.lastname: Serain
authors.affiliation: d Departamento de Biologia Vegetal, PPG BTPB and PPG BV, Instituto de Biologia , Universidade Estadual de Campinas (UNICAMP) , Campinas , Brazil.
authors.forename: Ricardo Brunelli
authors.initials: RB
authors.lastname: Tirico
authors.affiliation: d Departamento de Biologia Vegetal, PPG BTPB and PPG BV, Instituto de Biologia , Universidade Estadual de Campinas (UNICAMP) , Campinas , Brazil.
authors.forename: Alvaro José
authors.initials: AJ
authors.lastname: Hernández-Tasco
authors.affiliation: a Departamento de Química , Universidade Federal do Amazonas , Manaus , Brazil.
authors.forename: Emmanoel Vilaça
authors.initials: EV
authors.lastname: Costa
authors.affiliation: d Departamento de Biologia Vegetal, PPG BTPB and PPG BV, Instituto de Biologia , Universidade Estadual de Campinas (UNICAMP) , Campinas , Brazil.
authors.forename: Marcos José
authors.initials: MJ
authors.lastname: Salvador
mesh_terms: D029061:Annonaceae / Q000737:chemistry*; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000935:Antifungal Agents / Q000494:pharmacology; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D045744:Cell Line, Tumor; D005410:Flame Ionization; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology; D006094:Gram-Positive Bacteria / Q000187:drug effects; D006801:Humans; D008826:Microbial Sensitivity Tests; D000081222:Monocyclic Sesquiterpenes; D009822:Oils, Volatile / Q000032:analysis* / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*; D000081223:Polycyclic Sesquiterpenes; D012717:Sesquiterpenes; D045787:Sesquiterpenes, Eudesmane; D045789:Sesquiterpenes, Guaiane; D015003:Yeasts / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D000972:Antineoplastic Agents, Phytogenic; D000975:Antioxidants; D016166:Free Radical Scavengers; D000081222:Monocyclic Sesquiterpenes; D009822:Oils, Volatile; D000081223:Polycyclic Sesquiterpenes; D012717:Sesquiterpenes; D045787:Sesquiterpenes, Eudesmane; D045789:Sesquiterpenes, Guaiane; C499668:gamma-eudesmol; C051082:beta-eudesmol; C118684:alpha-eudesmol; C469146:guaiol; C531191:beta-bisabolene; C515179:caryophyllene oxide
keywords: Anaxagorea brevipes; Annonaceae; bioactivity; essential oil