title: Immature platelet fraction in hypertensive pregnancy.
abstract: BACKGROUND
  Imbalance in hemostatic mechanisms can occur during pregnancy with a tendency for hypercoagulability and increased thrombosis risk. Pregnant women with hypertensive disorder, especially preeclampsia, show alterations in platelet indexes. Immature platelet fraction (IPF) has been suggested as a sensitive index for monitoring changes in platelet production and destruction.
  
  
  OBJECTIVE
  To evaluate the IPF in patients diagnosed with a gestational hypertensive disorder (GHD).
  
  
  METHODS
  A cross-sectional study was conducted at an University Hospital to estimate maternal blood IPF index in 99 pregnant women, divided into three groups: normotensive pregnancy (NP), preeclampsia syndrome (PES), and non-proteinuric hypertensive pregnancy (nPHP). Following ethical approval and written informed consent, samples were collected from 33 NP, 34 PES, and 32 nPHP women. Platelet indexes were measured by fluorescent flow cytometry.
  
  
  RESULTS
  IPF and mean platelet volume (MPV) counts in GHD were significantly higher than in NP (IPF: 3.8, 2.4-5.1%; 8.6, 5.8-10.6%; 7.3, 4.2-10.2%; p < 0.001 and MPV: 10.6 ± 0.9 fL; 12.1 ± 1.0 fL; 11.6 ± 1.0 fL; p < 0.001 for NP, PES, and nPHP, respectively). No difference was detected between PES and nPHP groups. The distribution of patients with an IPF above 6.1%for NP, PES, and nPHP was 9%, 65%, and 43.8%, respectively (p < 0.001). IPF as a test to differentiate GHD from the controls achieved an area under the curve of 0.83 on a receiver operating characteristics curve.
  
  
  CONCLUSIONS
  A distinct profile in platelet indexes was detected in hypertensive pregnancies. It suggests that these markers could be used in daily routine as an additional tool in the management of pregnant women.
authors.affiliation: a Programa de Pos-Graduaçao em Medicina e Ciencias da Saude (Nefrologia), Faculdade de Medicina, Instituto de Pesquisas Biomedicas , Hospital São Lucas - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS) , Porto Alegre , RS , Brazil.|b Laboratorio de Patologia Clinica -Hospital São Lucas Hospital /Faculdade de Farmácia Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS) , Porto Alegre , RS , Brazil.
authors.forename: Daniela
authors.initials: D
authors.lastname: Moraes
authors.affiliation: b Laboratorio de Patologia Clinica -Hospital São Lucas Hospital /Faculdade de Farmácia Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS) , Porto Alegre , RS , Brazil.
authors.forename: Terezinha Paz
authors.initials: TP
authors.lastname: Munhoz
authors.affiliation: a Programa de Pos-Graduaçao em Medicina e Ciencias da Saude (Nefrologia), Faculdade de Medicina, Instituto de Pesquisas Biomedicas , Hospital São Lucas - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS) , Porto Alegre , RS , Brazil.
authors.forename: Bartira E
authors.initials: BE
authors.lastname: Pinheiro da Costa
authors.affiliation: a Programa de Pos-Graduaçao em Medicina e Ciencias da Saude (Nefrologia), Faculdade de Medicina, Instituto de Pesquisas Biomedicas , Hospital São Lucas - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS) , Porto Alegre , RS , Brazil.
authors.forename: Marta Ribeiro
authors.initials: MR
authors.lastname: Hentschke
authors.affiliation: a Programa de Pos-Graduaçao em Medicina e Ciencias da Saude (Nefrologia), Faculdade de Medicina, Instituto de Pesquisas Biomedicas , Hospital São Lucas - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS) , Porto Alegre , RS , Brazil.
authors.forename: Fernando
authors.initials: F
authors.lastname: Sontag
authors.affiliation: a Programa de Pos-Graduaçao em Medicina e Ciencias da Saude (Nefrologia), Faculdade de Medicina, Instituto de Pesquisas Biomedicas , Hospital São Lucas - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS) , Porto Alegre , RS , Brazil.
authors.forename: Luiza
authors.initials: L
authors.lastname: Silveira Lucas
authors.affiliation: a Programa de Pos-Graduaçao em Medicina e Ciencias da Saude (Nefrologia), Faculdade de Medicina, Instituto de Pesquisas Biomedicas , Hospital São Lucas - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS) , Porto Alegre , RS , Brazil.
authors.forename: Giovani
authors.initials: G
authors.lastname: Gadonski
authors.affiliation: a Programa de Pos-Graduaçao em Medicina e Ciencias da Saude (Nefrologia), Faculdade de Medicina, Instituto de Pesquisas Biomedicas , Hospital São Lucas - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS) , Porto Alegre , RS , Brazil.
authors.forename: Ivan Carlos
authors.initials: IC
authors.lastname: Antonello
authors.affiliation: a Programa de Pos-Graduaçao em Medicina e Ciencias da Saude (Nefrologia), Faculdade de Medicina, Instituto de Pesquisas Biomedicas , Hospital São Lucas - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS) , Porto Alegre , RS , Brazil.
authors.forename: Carlos E
authors.initials: CE
authors.lastname: Poli-de-Figueiredo
mesh_terms: D000293:Adolescent; D000328:Adult; D015415:Biomarkers; D001792:Blood Platelets / Q000378:metabolism*; D001794:Blood Pressure; D003430:Cross-Sectional Studies; D004909:Erythrocyte Indices; D005260:Female; D006801:Humans; D046110:Hypertension, Pregnancy-Induced / Q000097:blood* / Q000175:diagnosis; D063847:Mean Platelet Volume; D010976:Platelet Count; D011247:Pregnancy; D012372:ROC Curve; D055815:Young Adult
chemical_list: D015415:Biomarkers
keywords: Blood platelets; mean platelet volume; preeclampsia; pregnancy induced hypertension