title: Quantification of aroma constituents of mango sap from different Pakistan mango cultivars using gas chromatography triple quadrupole mass spectrometry.
abstract: In this study, a quantitative method was developed based on gas chromatography triple quadrupole mass spectrometry (GC-QQQ-MS) for the analysis of aroma component of mango sap (latex) in nine Pakistani varieties that are Anmol, Began pali, Badami, Caroba, Chaunsa, Lal patra, Neelum, Sohnara and Tota pari. The non-aqueous phase of sap was studied and a total seven selected terpenes that are α-pinene, α-phellandrene, (+)-3-carene, sabinene, γ-terpinene, (-)-trans-caryophyllene and α-humulene were quantified using GC-QQQ-MS in MRM (multiple reaction mode) mode. Calibration curves were generated and R(2)>0.99 was found for all analytes. Intra-day and inter-day precision (% R.S.D.) of the developed method was less than 4% while % accuracy was in the range of 95.3-105.1 for all analytes. Among all the varieties, Neelum contains the highest amount of four terpenes out of seven quantified terpenes.
authors.affiliation: H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi 75270, Pakistan. Electronic address: musharraf1977@yahoo.com.
authors.forename: Syed Ghulam
authors.initials: SG
authors.lastname: Musharraf
authors.affiliation: H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi 75270, Pakistan.
authors.forename: Jalal
authors.initials: J
authors.lastname: Uddin
authors.affiliation: H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi 75270, Pakistan.
authors.forename: Amna Jabbar
authors.initials: AJ
authors.lastname: Siddiqui
authors.affiliation: H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi 75270, Pakistan.
authors.forename: Muhammad Irfan
authors.initials: MI
authors.lastname: Akram
mesh_terms: D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D031022:Mangifera / Q000737:chemistry*; D010154:Pakistan; D012903:Smell; D013729:Terpenes / Q000737:chemistry*
chemical_list: D013729:Terpenes
keywords: Aroma volatile compounds; GC–QQQ-MS; Non-aqueous phase of sap; Quantification