title: Small angle light scattering assay for the detection of malaria infection.
abstract: The diagnosis of malaria, caused by Plasmodium spp., still remains a challenging process. Especially in low-income countries, a rapid user-friendly method is needed for the efficient care of the patient. A small-angle light scattering device consisting of hardware and software was developed. Using the DNA-binding dye SYBR Green, malaria infections could be distinguished in healthy red blood cells infected with Plasmodium. Subsequently, samples from parasite positive and negative patients living in a hyper-endemic area of Kinshasa, DRC were assessed. The scatter profiles were distinct and malaria infection could be detected using the Giemsa stain. Although these results are preliminary, they indicate that the device has the potential to be used as a new diagnostic tool for the detection of Malaria infection.
authors.affiliation: University of Antwerp, Faculty of Medicine and Health Sciences, International Health Unit, Universiteitsplein 1, 2610 Antwerp, Belgium.
authors.forename: R A
authors.initials: RA
authors.lastname: Inocêncio da Luz
authors.affiliation: University of Kinshasa, Faculty of Medicine, Department of Tropical Medicine, Kinshasa, Democratic Republic of the Congo; University of Kinshasa, Faculty of Medicine, Department of Tropical Medicine, Kinshasa, Democratic Republic of the Congo.
authors.forename: H M
authors.initials: HM
authors.lastname: Mavoko
authors.affiliation: Faculty of Pharmacy, University of Toronto, 144 College St., Toronto, Ontario, Canada M5S 2S2.
authors.forename: I
authors.initials: I
authors.lastname: Crandall
authors.affiliation: Spectradigital Corporation, Guelph, Ontario, Canada.
authors.forename: S
authors.initials: S
authors.lastname: Deshpande
authors.affiliation: University of Kinshasa, Faculty of Medicine, Department of Tropical Medicine, Kinshasa, Democratic Republic of the Congo.
authors.forename: P
authors.initials: P
authors.lastname: Lutumba
authors.affiliation: University of Antwerp, Faculty of Medicine and Health Sciences, International Health Unit, Universiteitsplein 1, 2610 Antwerp, Belgium. Electronic address: Jean-pierre.vangeertruyden@uantwerpen.be.
authors.forename: J P
authors.initials: JP
authors.lastname: Van geertruyden
mesh_terms: D001399:Azure Stains / Q000494:pharmacology; D052160:Benzothiazoles; D003959:Diamines; D004912:Erythrocytes / Q000469:parasitology*; D005456:Fluorescent Dyes / Q000494:pharmacology; D006801:Humans; D008027:Light; D016778:Malaria, Falciparum / Q000175:diagnosis*; D009930:Organic Chemicals / Q000494:pharmacology; D018512:Parasitemia / Q000175:diagnosis*; D010963:Plasmodium falciparum; D011804:Quinolines; D012542:Scattering, Radiation
chemical_list: D001399:Azure Stains; D052160:Benzothiazoles; D003959:Diamines; D005456:Fluorescent Dyes; D009930:Organic Chemicals; D011804:Quinolines; C098022:SYBR Green I
keywords: Light scatter; Malaria diagnosis; Plasmodium spp.; Spectrophotometer