title: [The Neisseria gonorhoeae and Chlamydia trachomatis coinfection in patients of Department of Dermatology and Venereology Medical University of Warsaw].
abstract: BACKGROUND
  The aim of the study was to investigate the coexistence of N. gonorrhoeae and C. trachomatis infections in patients of Department of Dermatology and Venereology in Warsaw.
  
  
  METHODS
  We investigated the urethral, cervical, anal and pharyngeal speci mens from 140 symptomatic and asymptomatic patients of Department of Dermatology and Venereology in Warsaw using the Real-Time PCR method. Real Time PCR DUPLICα® RealTime Neisseria gonorrhoeae and DUPLICα® RealTime Chlamydia trachomatis 2nd Generation Detection Kits (Euroclone®)was performed on termocycler Smart Cycler® Dx. For DNA isolation the Bact Extra Pure Kit (Euroclone®) was used.
  
  
  RESULTS
  22 samples were positive for C. trachomatis and 28 were positive for N. gonorrhoeae. Both infections coexisted in nine patients (6.4%). In our investigations, in opposition to results from other centers, gonorrhoea was more prevalent than C. trachomatis infection. The chlamydial infection coexisted in 32.1% with gonorrhea and gonorrhea coexisted with chlamydial infection in 40.9%.
  
  
  CONCLUSIONS
  The coinfection with Neisseria gonorrhoeae and Chlamydia trachomatis occurs very often. According IUSTI and CDC recommendations patients with one kind of sexually transmitted disease (STI) diagnosed should be tested for the others. It is especially true in the case of gonorrhea and Chlamydia trachomatis infection. In IUSTI and CDC recommendations treatment for Chlamydia trachomatis is indicated in patients with gonorrhea.
authors.forename: Beata
authors.initials: B
authors.lastname: Młynarczyk-Bonikowska
authors.forename: Ewa
authors.initials: E
authors.lastname: Skulska
authors.forename: Szymon
authors.initials: S
authors.lastname: Walter de Walthoffen
authors.forename: Grażyna
authors.initials: G
authors.lastname: Młynarczyk
authors.forename: Magdalena
authors.initials: M
authors.lastname: Malejczyk
authors.forename: Sławomir
authors.initials: S
authors.lastname: Majewski
mesh_terms: D000328:Adult; D002584:Cervix Uteri / Q000382:microbiology; D002690:Chlamydia Infections / Q000150:complications* / Q000382:microbiology*; D002692:Chlamydia trachomatis / Q000302:isolation & purification*; D060085:Coinfection / Q000175:diagnosis* / Q000453:epidemiology* / Q000382:microbiology; D005260:Female; D006069:Gonorrhea / Q000150:complications* / Q000382:microbiology*; D006801:Humans; D008297:Male; D009344:Neisseria gonorrhoeae / Q000302:isolation & purification; D010614:Pharynx / Q000382:microbiology; D015995:Prevalence; D014521:Urethra / Q000382:microbiology