title: [Expressions of WWOX and CD133 in colorectal cancer and their clinical significance].
abstract: OBJECTIVE
  To investigate the expressions of WWOX and CD133 in colorectal cancer (CRC) and their relationship with the clinicopathologic characteristics of CRC.
  
  
  METHODS
  The expressions of WWOX and CD133 proteins were examined by immunohistochemistry in 174 specimens of CRC tissues and 80 normal colorectal mucosa tissues.
  
  
  RESULTS
  The positivity rates of WWOX and CD133 proteins were 41.4% and 53.4% in CRC tissues, respectively, significantly different from the rates in normal colorectal mucosa tissues (87.5% and 5.0%, respectively; P<0.05). WWOX and CD133 protein expressions were signi- ficantly correlated with the histological grades of the tumors, depth of invasion, lymph node metastasis, and Duke's stages (P<0.05). Spearman analysis showed a negative relationship between the WWOX expression and CD133 expression (P<0.05). Kaplan-Meier survival analysis showed that the overall survival time of CRC patients with a positive expression of WWOX was longer than that of patients with a negative expression of WWOX; the overall survival time of patients with a positive expression of CD133 was shorter than that of the negative patients (P<0.05). COX regression analysis identified positive expressions of WWOX and CD133 protein and Duke's stage as the independent prognostic factors of CRC.
  
  
  CONCLUSIONS
  Abnormal expressions of WWOX and CD133 might be involved in the initiation, development, invasion, and metastasis of CRC. A combined detection of WWOX and CD133 can help in predicting the progression and prognosis of CRC.
authors.affiliation: Department of Pathology, First Affiliated Hospital of Bengbu Medical College, Bengbu 233000, China.E-mail: 35736791@qq.com.
authors.forename: Bo
authors.initials: B
authors.lastname: Zhu
authors.forename: Danna
authors.initials: D
authors.lastname: Wang
authors.forename: Qiong
authors.initials: Q
authors.lastname: Zhang
authors.forename: Shiwu
authors.initials: S
authors.lastname: Wu
authors.forename: Lan
authors.initials: L
authors.lastname: Yu
authors.forename: Yisheng
authors.initials: Y
authors.lastname: Tao
mesh_terms: D000071916:AC133 Antigen; D015703:Antigens, CD / Q000378:metabolism*; D015179:Colorectal Neoplasms / Q000378:metabolism*; D018450:Disease Progression; D006023:Glycoproteins / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008207:Lymphatic Metastasis; D010088:Oxidoreductases / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism*; D011379:Prognosis; D025521:Tumor Suppressor Proteins / Q000378:metabolism*; D000074584:WW Domain-Containing Oxidoreductase
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D006023:Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides; D025521:Tumor Suppressor Proteins; D010088:Oxidoreductases; D000074584:WW Domain-Containing Oxidoreductase; C486071:WWOX protein, human