title: microRNA-762 promotes breast cancer cell proliferation and invasion by targeting IRF7 expression.
abstract: OBJECTIVE
  miRNAs play crucial roles in human tumourigenesis. This study was performed to measure expression and function of miR-762 in breast cancer.
  
  
  METHODS
  Expression of miR-762 in breast tissues and cell lines (SK-BR-3, DA-MB-435s, MCF-7 and MDA-MB-231, HBL-100) was measured by using real-time RT-PCR. We restored expression of miR-762 in MCF-7 cells to measure its functional roles. Luciferase assays were performed to reveal the target gene of miR-762.
  
  
  RESULTS
  Expression of miR-762 was high in both breast cancer cell lines and specimens, and its overexpression increased breast cancer cell proliferation and invasion. Interferon regulatory factor 7 (IRF7) is a direct target of miR-762 and overexpression of miR-762 reduced expression of IRF7. Moreover, IRF7 was repressed, its levels inversely correlated to miR-762 expression. IRF7 rescued miR-762-induced cell invasion and proliferation.
  
  
  CONCLUSIONS
  These results demonstrate that miR-762 tumour effect was achieved by targeting IRF7 in human breast cancer specimens.
authors.affiliation: Department of Thyroid, Breast and Vascular Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaan Xi, 710032, China.
authors.forename: Yongping
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of occupational and environmental Health, School of Public Health, Central South University, Changsha, HuNan, 410078, China.
authors.forename: Ruixue
authors.initials: R
authors.lastname: Huang
authors.affiliation: Department of Thyroid, Breast and Vascular Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaan Xi, 710032, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Thyroid, Breast and Vascular Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaan Xi, 710032, China.
authors.forename: Junsheng
authors.initials: J
authors.lastname: Hao
authors.affiliation: Department of Oncology, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaan Xi, 710032, China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Department of Thyroid, Breast and Vascular Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaan Xi, 710032, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Ling
authors.affiliation: Department of Thyroid, Breast and Vascular Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaan Xi, 710032, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Yun
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D050839:Interferon Regulatory Factor-7 / Q000096:biosynthesis*; D061986:MCF-7 Cells; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics*; D009361:Neoplasm Invasiveness / Q000235:genetics* / Q000473:pathology; D009362:Neoplasm Metastasis / Q000235:genetics* / Q000473:pathology
chemical_list: C494234:IRF7 protein, human; D050839:Interferon Regulatory Factor-7; C583095:MIRN762 microRNA, human; D035683:MicroRNAs