title: Methane attenuates myocardial ischemia injury in rats through anti-oxidative, anti-apoptotic and anti-inflammatory actions.
abstract: Myocardial infarction (MI) remains the most frequent cardiovascular disease with high mortality. Recently, methane has been shown protective effects on small intestinal ischemia-reperfusion injury. We hypothesized that methane-rich saline (MS) could protect the myocardium again MI via its anti-oxidative, anti-apoptotic and anti-inflammatory effects. In experiment 1, tetrazolium chloride staining and detection of myocardial enzymes and oxidative and inflammatory parameters were performed at 12h after MI to determine the optimal dose at which intraperitoneal MS exerted the best protective effects on MI. In experiment 2, rats were treated with 10 ml/kg MS. Myocyte apoptosis was detected 72 h after MI, and cardiac function and myocardial remodeling were evaluated 4 weeks after MI. Results showed different dose of MS reduced infarct area, decreased myocardial enzymes, inhibited inflammation and oxidative stress following MI. The optimal dose of MS was 10 mg/kg. Moreover, treatment with 10mg/kg MS for 3 days significantly reduced myocyte apoptosis, improved cardiac function and inhibited myocardial remodeling (reduced anterior wall thickness, attenuated myocyte hypertrophy, and decreased myocardial collagen). MS protects the myocardium of MI rats via its anti-oxidative, anti-inflammatory, anti-apoptotic and anti-remodeling activities. Thus, MS provides a novel and promising strategy for the treatment of ischemic heart diseases.
authors.affiliation: Department of Navy Aviation Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai 200433, People's Republic of China; Graduate Management Unit, Second Military Medical University, Shanghai 200433, People's Republic of China.
authors.forename: Ouyang
authors.initials: O
authors.lastname: Chen
authors.affiliation: Department of Navy Aviation Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai 200433, People's Republic of China.
authors.forename: Zhouheng
authors.initials: Z
authors.lastname: Ye
authors.affiliation: Department of General Internal Medicine, Branch of 411 Hospital of People's Liberation Army, Shanghai 200083, People's Republic of China.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Cao
authors.affiliation: Department of Physiology and Pharmacology, Loma Linda University Medical Center, Loma Linda, CA, USA.
authors.forename: Anatol
authors.initials: A
authors.lastname: Manaenko
authors.affiliation: Graduate Management Unit, Second Military Medical University, Shanghai 200433, People's Republic of China.
authors.forename: Ke
authors.initials: K
authors.lastname: Ning
authors.affiliation: Graduate Management Unit, Changhai Hospital Affiliated to the Second Military Medical University, Shanghai 200433, People's Republic of China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Zhai
authors.affiliation: Department of Navy Aviation Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai 200433, People's Republic of China.
authors.forename: Rongjia
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Department of Navy Aviation Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai 200433, People's Republic of China.
authors.forename: Ting
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Department of Orthopedics Trauma, Changhai Hospital Affiliated to the Second Military Medical University, Shanghai 200433, People 's Republic of China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Diving Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai 200433, People's Republic of China. Electronic address: liuwenwu1980@hotmail.com.
authors.forename: Wenwu
authors.initials: W
authors.lastname: Liu
authors.affiliation: Department of Navy Aviation Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai 200433, People's Republic of China. Electronic address: sunxjk@hotmail.com.
authors.forename: Xuejun
authors.initials: X
authors.lastname: Sun
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002245:Carbon Dioxide / Q000097:blood; D006439:Hemodynamics / Q000187:drug effects; D008297:Male; D008697:Methane / Q000493:pharmacokinetics / Q000494:pharmacology*; D017202:Myocardial Ischemia / Q000188:drug therapy* / Q000401:mortality / Q000503:physiopathology; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D002245:Carbon Dioxide; D009195:Peroxidase; D008697:Methane
keywords: Anti-apoptosis; Anti-inflammation; Anti-oxidation; Methane; Myocardial infraction