title: Improved oxidation of aromatic and aliphatic hydrocarbons using rate enhancing variants of P450Bm3 in combination with decoy molecules.
abstract: Enzyme performance can be improved using decoy molecules or engineered variants to accelerate the activity without affecting selectivity. Here we combine a rate accelerator variant of cytochrome P450Bm3 with decoy molecules to enhance the oxidation activity of a range of small organic molecules. This combined approach offers superior biocatalytic efficiency without modifying the product distribution.
authors.affiliation: Department of Chemistry, University of Adelaide, SA 5005, Australia. stephen.bell@adelaide.edu.au.
authors.forename: Samuel D
authors.initials: SD
authors.lastname: Munday
authors.affiliation: Department of Chemistry, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8602, Japan.
authors.forename: Osami
authors.initials: O
authors.lastname: Shoji
authors.affiliation: Department of Chemistry, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8602, Japan.
authors.forename: Yoshihito
authors.initials: Y
authors.lastname: Watanabe
authors.affiliation: Department of Chemistry, University of Oxford, Inorganic Chemistry Laboratory, South Parks Road, Oxford, OX1 3QR, UK.
authors.forename: Luet-Lok
authors.initials: LL
authors.lastname: Wong
authors.affiliation: Department of Chemistry, University of Adelaide, SA 5005, Australia. stephen.bell@adelaide.edu.au.
authors.forename: Stephen G
authors.initials: SG
authors.lastname: Bell