title: Loss of the Mediator subunit Med20 affects transcription of tRNA and other non-coding RNA genes in fission yeast.
abstract: Mediator is a co-regulator of RNA polymerase II (Pol II), transducing signals from regulatory elements and transcription factors to the general transcription machinery at the promoter. We here demonstrate that Med20 influences ribosomal protein expression in fission yeast. In addition, loss of Med20 leads to an accumulation of aberrant, readthrough tRNA transcripts. These transcripts are polyadenylated and targeted for degradation by the exosome. Similarly, other non-coding RNA molecules, such as snRNA, snoRNA and rRNA, are also enriched in the polyadenylate preparations in the absence of Med20. We suggest that fission yeast Mediator takes part in a regulatory pathway that affects Pol III-dependent transcripts.
authors.affiliation: Institute of Biomedicine, University of Gothenburg, P.O. Box 440, SE-405 30 Gothenburg, Sweden.
authors.forename: Jonas O P
authors.initials: JO
authors.lastname: Carlsten
authors.affiliation: Institute of Biomedicine, University of Gothenburg, P.O. Box 440, SE-405 30 Gothenburg, Sweden.
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Zhu
authors.affiliation: Institute of Biomedicine, University of Gothenburg, P.O. Box 440, SE-405 30 Gothenburg, Sweden.
authors.forename: Marcela Dávila
authors.initials: MD
authors.lastname: López
authors.affiliation: Institute of Biomedicine, University of Gothenburg, P.O. Box 440, SE-405 30 Gothenburg, Sweden.
authors.forename: Tore
authors.initials: T
authors.lastname: Samuelsson
authors.affiliation: Institute of Biomedicine, University of Gothenburg, P.O. Box 440, SE-405 30 Gothenburg, Sweden. Electronic address: claes.gustafsson@medkem.gu.se.
authors.forename: Claes M
authors.initials: CM
authors.lastname: Gustafsson
mesh_terms: D056892:Mediator Complex / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012319:RNA Polymerase II / Q000235:genetics; D012343:RNA, Transfer / Q000096:biosynthesis* / Q000235:genetics; D022661:RNA, Untranslated / Q000096:biosynthesis* / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics; D012568:Schizosaccharomyces / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D056892:Mediator Complex; D022661:RNA, Untranslated; D014157:Transcription Factors; D012343:RNA, Transfer; D012319:RNA Polymerase II
keywords: Exosome; Mediator; RNA polymerase III; Transcription; tRNA