title: Gold nanoclusters-based chemiluminescence resonance energy transfer method for sensitive and label-free detection of trypsin.
abstract: A chemiluminescence resonance energy transfer (CRET) platform was developed for sensitive and label-free detection of protease by using trypsin as a model analyte. In this CRET platform, bis(2,4,6-trichlorophenyl)oxalate-hydrogen peroxide chemiluminescence (CL) reaction was utilized as an energy donor and bovine serum albumin (BSA)-stabilized gold nanoclusters (Au NCs) as an energy acceptor. The BSA-stabilized Au NCs triggered the CRET phenomenon by accepting the energy from TCPO-H2O2 CL reaction, thus producing intense CL. In the presence of trypsin, the protein template of BSA-stabilized Au NCs was digested, which frustrated the energy transfer efficiency between the CL donor and the BSA-stabilized Au NCs, leading to a significant decrease in the CL signal. The decreased CL signal was proportional to the logarithm of trypsin concentration in the range of 0.01-50.0µg mL(-1). The detection limit for trypsin was 9ng mL(-)(1) and the relative standard deviations were lesser than 3% (n=11). This Au NCs-based CRET platform was successfully applied to the determination of trypsin in human urine samples, demonstrating its potential application in clinical diagnosis.
authors.affiliation: Department of Chemistry, School of Science, Xi'an Jiaotong University, Xi'an 710049, China.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: You
authors.affiliation: Department of Chemistry, School of Science, Xi'an Jiaotong University, Xi'an 710049, China. Electronic address: liyh@mail.xjtu.edu.cn.
authors.forename: Yinhuan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Chemistry, School of Science, Xi'an Jiaotong University, Xi'an 710049, China.
authors.forename: Baoping
authors.initials: B
authors.lastname: Li
authors.affiliation: Department of Genetics and Molecular Biology, School of Medicine, Xi'an Jiaotong University, Xi'an 710049, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Ma
mesh_terms: D000818:Animals; D002417:Cattle; D006046:Gold / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D049449:Luminescence; D053768:Metal Nanoparticles; D015394:Molecular Structure; D012710:Serum Albumin, Bovine; D013194:Staining and Labeling; D014357:Trypsin / Q000737:chemistry / Q000652:urine*; D016482:Urinalysis / Q000379:methods*
chemical_list: D012710:Serum Albumin, Bovine; D006046:Gold; D014357:Trypsin
keywords: Chemiluminescence; Gold nanoclusters; Resonance energy transfer; Trypsin