title: A metallo-keratinase from a newly isolated Acinetobacter sp. R-1 with low collagenase activity and its biotechnological application potential in leather industry.
abstract: Microbial keratinase is a well-recognized enzyme that can specifically degrade insoluble keratins. A keratinase-producing bacterium was isolated from a duck ranch soil and identified as Acinetobacter sp. R-1 based on the biochemical characteristics and 16S rDNA gene sequencing. It showed high keratinase activity and low collagenase activity. The keratinase was purified to electrophoretic homogeneity with 6.69% recovery, 2.68-fold purification and an estimated molecular weight of 25 kDa. Additionally, the keratinase showed optimal activity at 50 °C and pH11. Keratinase activity of Acinetobacter sp. significantly increased in the presence of Li(+), Na(+), and Ca(2+), while it was completely inhibited by EDTA, indicating it was a metallo-keratinase. Moreover, the crude keratinase from Acinetobacter sp. R-1 could thoroughly depilate goat skin and simultaneously modify the wool surface, which indicated its applicable potential in leather and textile industries.
authors.affiliation: School of Pharmaceutical Science, Jiangnan University, Wuxi, 214122, People's Republic of China.|The Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi, 214122, People's Republic of China.
authors.forename: Rong-Xian
authors.initials: RX
authors.lastname: Zhang
authors.affiliation: School of Pharmaceutical Science, Jiangnan University, Wuxi, 214122, People's Republic of China.
authors.forename: Jin-Song
authors.initials: JS
authors.lastname: Gong
authors.affiliation: School of Pharmaceutical Science, Jiangnan University, Wuxi, 214122, People's Republic of China.
authors.forename: Dan-Dan
authors.initials: DD
authors.lastname: Zhang
authors.affiliation: School of Pharmaceutical Science, Jiangnan University, Wuxi, 214122, People's Republic of China.
authors.forename: Chang
authors.initials: C
authors.lastname: Su
authors.affiliation: School of Pharmaceutical Science, Jiangnan University, Wuxi, 214122, People's Republic of China.
authors.forename: Ying-Shuo
authors.initials: YS
authors.lastname: Hou
authors.affiliation: School of Pharmaceutical Science, Jiangnan University, Wuxi, 214122, People's Republic of China.
authors.forename: Heng
authors.initials: H
authors.lastname: Li
authors.affiliation: School of Pharmaceutical Science, Jiangnan University, Wuxi, 214122, People's Republic of China. shijs@163.com.
authors.forename: Jin-Song
authors.initials: JS
authors.lastname: Shi
authors.affiliation: School of Pharmaceutical Science, Jiangnan University, Wuxi, 214122, People's Republic of China. zhenghxu@jiangnan.edu.cn.|The Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi, 214122, People's Republic of China. zhenghxu@jiangnan.edu.cn.
authors.forename: Zheng-Hong
authors.initials: ZH
authors.lastname: Xu
mesh_terms: D000150:Acinetobacter / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification; D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D017364:Collagenases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D006041:Goats; D045726:Metalloproteases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D010447:Peptide Hydrolases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D012867:Skin; D013783:Textile Industry; D014935:Wool
chemical_list: D001426:Bacterial Proteins; D045726:Metalloproteases; D010447:Peptide Hydrolases; C035855:keratinase; D017364:Collagenases
keywords: Acinetobacter sp. R-1; Dehairing; Keratinase; Purification; Surface modification