title: One-pot preparation of a novel monolith for high performance liquid chromatography applications.
abstract: Various novel porous organic-based monoliths with the mode of hydrophobicity were synthesized by in situ free-radical crosslinking copolymerization and optimized for the separations of small molecules and high-performance reversed-phase chromatography (RP-chromatography). These monoliths contained co-polymers based on glycidyl methacrylate (GMA)/ethylene glycol dimethacrylate (EDMA)/tripropylene glycol diacrylate (TPGDA) or EDMA/TPGDA. A mixture of cetanol, methanol and poly (ethylene glycol) (PEG) was used as the porogen, with the ratio of these solvents being varied along with the polymerization temperature to generate a library of monoliths. The conditions were optimized and the resulting poly (GMA-co-TPGDA-co-EDMA) monolith was investigated by infrared spectrometer (IR), field emission scanning electron microscope (FESEM), and mercury intrusion porosimetry (MIP), respectively. The column performance was assessed by the separation of a series of neutral solutes of benzene derivatives. The result demonstrated that the prepared monolith exhibited an RP-chromatographic behavior and relatively homogeneous structure, good permeability and separation performance. Moreover, the relative standard deviations (RSDs) of the retention factor values for benzene derivatives were less than 1.5% (n=7, column-to-column). The approach used in this study was extended to the separation of anilines.
authors.affiliation: Key Laboratory of Analytical Science and Technology of Hebei Province, College of Chemistry and Environmental Science, Hebei University, Baoding 071002, China; College of Science, Hebei North University, Zhangjiakou, 075000, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Jiao
authors.affiliation: Key Laboratory of Analytical Science and Technology of Hebei Province, College of Chemistry and Environmental Science, Hebei University, Baoding 071002, China. Electronic address: shensg@hbu.cn.
authors.forename: Shigang
authors.initials: S
authors.lastname: Shen
authors.affiliation: Key Laboratory of Analytical Science and Technology of Hebei Province, College of Chemistry and Environmental Science, Hebei University, Baoding 071002, China.
authors.forename: Tiesheng
authors.initials: T
authors.lastname: Shi
mesh_terms: D001555:Benzene Derivatives / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D056148:Chromatography, Reverse-Phase / Q000295:instrumentation*; D008855:Microscopy, Electron, Scanning; D015203:Reproducibility of Results; D013055:Spectrophotometry, Infrared
chemical_list: D001555:Benzene Derivatives
keywords: High performance liquid chromatography (HPLC); Mixed-mode polymer monolith; Organic polymeric monolith; Small molecules; Tripropylene glycol diacrylate