title: A new tobamovirus infecting tomato crops in Jordan.
abstract: In this study, we completed the whole genome sequence of a new tobamovirus isolated from tomato plants grown in greenhouses in Jordan during the spring of 2015. The 6393-nt single-stranded RNA (ssRNA) genome encodes four proteins, as do other tobamoviruses: two replication-related proteins of 126 kDa and 183 kDa, a 30-kDa movement protein (MP) and a 17.5-kDa coat protein (CP). Phylogenetic analysis showed that this virus does not group with either the tomato mosaic virus (ToMV) or the tobacco mosaic virus (TMV) clades. Instead, it stems from a branch leading to the TMV clade. Analysis of possible recombination events between this virus and representative isolates of closely related tomato-infecting tobamoviruses showed that at least one region originated by recombination. We provide evidence that we have identified a new tobamovirus, for which we propose the name "tomato brown rugose fruit virus".
authors.affiliation: Department of Plant Protection, Faculty of Agriculture, The University of Jordan, Amman, 11942, Jordan.
authors.forename: N
authors.initials: N
authors.lastname: Salem
authors.affiliation: Department of Plant Protection, Faculty of Agriculture, The University of Jordan, Amman, 11942, Jordan.
authors.forename: A
authors.initials: A
authors.lastname: Mansour
authors.affiliation: Istituto per la Protezione Sostenibile delle Piante, Sez. di Torino, CNR, Strada delle Cacce 73, 10135, Turin, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Ciuffo
authors.affiliation: Department of Plant Pathology, University of California, Davis, CA, 95616, USA.
authors.forename: B W
authors.initials: BW
authors.lastname: Falk
authors.affiliation: Istituto per la Protezione Sostenibile delle Piante, Sez. di Torino, CNR, Strada delle Cacce 73, 10135, Turin, Italy. m.turina@ivv.cnr.it.
authors.forename: M
authors.identifier: http://orcid.org/0000-0002-9659-9470
authors.initials: M
authors.lastname: Turina
mesh_terms: D016000:Cluster Analysis; D023061:Gene Order; D016679:Genome, Viral; D007597:Jordan; D018551:Solanum lycopersicum / Q000821:virology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D017898:Tobamovirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D014764:Viral Proteins / Q000235:genetics
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins