title: Effects of physical exercise training in DNA damage and repair activity in humans with different genetic polymorphisms of hOGG1 (Ser326Cys).
abstract: The main purpose of this pilot study was to investigate the possible influence of genetic polymorphisms of the hOGG1 (Ser326Cys) gene in DNA damage and repair activity by 8-oxoguanine DNA glycosylase 1 (OGG1 enzyme) in response to 16 weeks of combined physical exercise training. Thirty-two healthy Caucasian men (40-74 years old) were enrolled in this study. All the subjects were submitted to a training of 16 weeks of combined physical exercise. The subjects with Ser/Ser genotype were considered as wild-type group (WTG), and Ser/Cys and Cys/Cys genotype were analysed together as mutant group (MG). We used comet assay in conjunction with formamidopyrimidine DNA glycoslyase (FPG) to analyse both strand breaks and FPG-sensitive sites. DNA repair activity were also analysed with the comet assay technique. Our results showed no differences between DNA damage (both strand breaks and FPG-sensitive sites) and repair activity (OGG1) between genotype groups (in the pre-training condition). Regarding the possible influence of genotype in the response to 16 weeks of physical exercise training, the results revealed a decrease in DNA strand breaks in both groups, a decrease in FPG-sensitive sites and an increase in total antioxidant capacity in the WTG, but no changes were found in MG. No significant changes in DNA repair activity was observed in both genotype groups with physical exercise training. This preliminary study suggests the possibility of different responses in DNA damage to the physical exercise training, considering the hOGG1 Ser326Cys polymorphism.
authors.affiliation: University of Trás-os-Montes and Alto Douro, Vila Real, Portugal.|Research Center in Sports Sciences, Health and Human Development, CIDESD, UTAD, Vila Real, Portugal.
authors.forename: Jorge Pinto
authors.initials: JP
authors.lastname: Soares
authors.affiliation: University of Trás-os-Montes and Alto Douro, Vila Real, Portugal.
authors.forename: Ana Inês
authors.initials: AI
authors.lastname: Silva
authors.affiliation: University of Trás-os-Montes and Alto Douro, Vila Real, Portugal.|Centre for Research and Technology of Agro-Environmental and Biological Sciences, CITAB, UTAD, Vila Real, Portugal.
authors.forename: Amélia M
authors.initials: AM
authors.lastname: Silva
authors.affiliation: University of Trás-os-Montes and Alto Douro, Vila Real, Portugal.|Research Center in Sports Sciences, Health and Human Development, CIDESD, UTAD, Vila Real, Portugal.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Almeida
authors.affiliation: Department of Environment Health, Portuguese National Institute of Health, Rua Alexandre Herculano 321, Porto 4000-055, Portugal.
authors.forename: João Paulo
authors.initials: JP
authors.lastname: Teixeira
authors.affiliation: University of Trás-os-Montes and Alto Douro, Vila Real, Portugal.|Centre of Agricultural Genomics and Biotechnology, CGBA, UTAD, Vila Real, Portugal.
authors.forename: Manuela
authors.initials: M
authors.lastname: Matos
authors.affiliation: University of Trás-os-Montes and Alto Douro, Vila Real, Portugal.|Animal and Veterinary Research Centre, CECAV, UTAD, Vila Real, Portugal.
authors.forename: Isabel
authors.initials: I
authors.lastname: Gaivão
authors.affiliation: University of Trás-os-Montes and Alto Douro, Vila Real, Portugal.|Research Center in Sports Sciences, Health and Human Development, CIDESD, UTAD, Vila Real, Portugal.
authors.forename: Maria Paula
authors.initials: MP
authors.lastname: Mota
mesh_terms: D000328:Adult; D000368:Aged; D000886:Anthropometry; D004249:DNA Damage; D045647:DNA Glycosylases / Q000235:genetics*; D004260:DNA Repair; D015444:Exercise; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation / Q000235:genetics; D010809:Physical Fitness; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*
chemical_list: D045647:DNA Glycosylases; C479058:oxoguanine glycosylase 1, human
keywords: DNA damage; DNA repair activity; aerobic capacity; hOGG1 polymorphism; physical exercise training; strength