title: Porphyromonas gingivalis Fim-A genotype distribution among Colombians.
abstract: BACKGROUND
  Porphyromonas gingivalis is associated with periodontitis and exhibit a wide array of virulence factors, including fimbriae which is encoded by the FimA gene representing six known genotypes.
  
  
  OBJECTIVE
  To identify FimA genotypes of P. gingivalis in subjects from Cali-Colombia, including the co-infection with Aggregatibacter actinomycetemcomitans, Treponema denticola, and Tannerella forsythia.
  
  
  METHODS
  Subgingival samples were collected from 151 people exhibiting diverse periodontal condition. The occurrence of P. gingivalis, FimA genotypes and other bacteria was determined by PCR.
  
  
  RESULTS
  P. gingivalis was positive in 85 patients. Genotype FimA II was more prevalent without reach significant differences among study groups (54.3%), FimA IV was also prevalent in gingivitis (13.0%). A high correlation (p= 0.000) was found among P. gingivalis, T. denticola, and T. forsythia co-infection. The FimA II genotype correlated with concomitant detection of T. denticola and T. forsythia.
  
  
  CONCLUSIONS
  Porphyromonas gingivalis was high even in the healthy group at the study population. A trend toward a greater frequency of FimA II genotype in patients with moderate and severe periodontitis was determined. The FimA II genotype was also associated with increased pocket depth, greater loss of attachment level, and patients co-infected with T. denticola and T. forsythia.
authors.affiliation: School of Dentistry, University of Valle, Cali Colombia ; Periodontal Medicine Group, University of Valle. Cali, Colombia.
authors.forename: Sandra
authors.initials: S
authors.lastname: Moreno
authors.affiliation: School of Dentistry, University of Valle, Cali Colombia ; Periodontal Medicine Group, University of Valle. Cali, Colombia.
authors.forename: Adriana
authors.initials: A
authors.lastname: Jaramillo
authors.affiliation: Periodontal Medicine Group, University of Valle. Cali, Colombia ; Department of Microbiology, University of Valle, Cali Colombia.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Parra
authors.affiliation: Faculty of Dentistry, University of Antioquia, Medellin Colombia.
authors.forename: Javier Enrique
authors.initials: JE
authors.lastname: Botero
authors.affiliation: School of Dentistry, University of Valle, Cali Colombia ; Periodontal Medicine Group, University of Valle. Cali, Colombia.
authors.forename: Adolfo
authors.initials: A
authors.lastname: Contreras
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016976:Aggregatibacter actinomycetemcomitans / Q000302:isolation & purification; D016866:Bacteroidaceae Infections / Q000453:epidemiology* / Q000382:microbiology; D041963:Bacteroidetes / Q000302:isolation & purification; D060085:Coinfection; D003105:Colombia / Q000453:epidemiology; D003430:Cross-Sectional Studies; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D016871:Pasteurellaceae Infections / Q000453:epidemiology / Q000382:microbiology; D010518:Periodontitis / Q000453:epidemiology* / Q000382:microbiology; D016966:Porphyromonas gingivalis / Q000235:genetics / Q000302:isolation & purification*; D048373:Treponema denticola / Q000302:isolation & purification; D055815:Young Adult
keywords: Porphyromonas gingivalis; bacterial fimbriae; gingivitis; periodontitis; polymerase chain reaction