title: Sex-specific age association with primary DNA transfer.
abstract: Practicing forensic scientists who are called to provide expert witness testimony are often asked to explain both the presence and the absence of DNA on objects that have been handled by perpetrators with bare hands. Unwashed hands, depending on what they have come in contact with previously, may become the vehicle of both primary and secondary transfer of DNA. In this study, we investigated the propensity of primary and secondary transfer of DNA from unwashed bare hands of 128 individuals onto plastic tubes. Our experiments, carried out in triplicate, have shown that DNA was not detected on all the touched tubes, secondary transfer of DNA, through unwashed hands, was small, and in the majority of cases primary DNA transfer could be distinguished from secondary DNA transfer. A statistically significant association was demonstrated between percent DNA profile deposited on plastic tubes, through unwashed hands, and the age of male individuals.
authors.affiliation: Laboratory of Forensic Genetics, The Cyprus Institute of Neurology and Genetics, 6 Airport Avenue, 2370, Nicosia, Cyprus.
authors.forename: Panayiotis
authors.initials: P
authors.lastname: Manoli
authors.affiliation: Laboratory of Forensic Genetics, The Cyprus Institute of Neurology and Genetics, 6 Airport Avenue, 2370, Nicosia, Cyprus.|Cyprus School of Molecular Medicine, The Cyprus Institute of Neurology and Genetics, 6 Airport Avenue, 2370, Nicosia, Cyprus.
authors.forename: Antonis
authors.initials: A
authors.lastname: Antoniou
authors.affiliation: Laboratory of Forensic Genetics, The Cyprus Institute of Neurology and Genetics, 6 Airport Avenue, 2370, Nicosia, Cyprus.|Cyprus School of Molecular Medicine, The Cyprus Institute of Neurology and Genetics, 6 Airport Avenue, 2370, Nicosia, Cyprus.
authors.forename: Evy
authors.initials: E
authors.lastname: Bashiardes
authors.affiliation: Laboratory of Forensic Genetics, The Cyprus Institute of Neurology and Genetics, 6 Airport Avenue, 2370, Nicosia, Cyprus.|Cyprus School of Molecular Medicine, The Cyprus Institute of Neurology and Genetics, 6 Airport Avenue, 2370, Nicosia, Cyprus.
authors.forename: Stavroulla
authors.initials: S
authors.lastname: Xenophontos
authors.affiliation: Cyprus School of Molecular Medicine, The Cyprus Institute of Neurology and Genetics, 6 Airport Avenue, 2370, Nicosia, Cyprus.
authors.forename: Marinos
authors.initials: M
authors.lastname: Photiades
authors.affiliation: Laboratory of Forensic Genetics, The Cyprus Institute of Neurology and Genetics, 6 Airport Avenue, 2370, Nicosia, Cyprus.
authors.forename: Vaso
authors.initials: V
authors.lastname: Stribley
authors.affiliation: Laboratory of Forensic Genetics, The Cyprus Institute of Neurology and Genetics, 6 Airport Avenue, 2370, Nicosia, Cyprus.
authors.forename: Michalis
authors.initials: M
authors.lastname: Mylona
authors.affiliation: Department of Electron Microscopy and Molecular pathology, The Cyprus Institute of Neurology and Genetics, 6 Airport Avenue, 2370, Nicosia, Cyprus.|Cyprus School of Molecular Medicine, The Cyprus Institute of Neurology and Genetics, 6 Airport Avenue, 2370, Nicosia, Cyprus.
authors.forename: Christiana
authors.initials: C
authors.lastname: Demetriou
authors.affiliation: Laboratory of Forensic Genetics, The Cyprus Institute of Neurology and Genetics, 6 Airport Avenue, 2370, Nicosia, Cyprus. cariolou@cing.ac.cy.|Cyprus School of Molecular Medicine, The Cyprus Institute of Neurology and Genetics, 6 Airport Avenue, 2370, Nicosia, Cyprus. cariolou@cing.ac.cy.
authors.forename: Marios A
authors.initials: MA
authors.lastname: Cariolou
mesh_terms: D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D000483:Alleles; D004247:DNA / Q000032:analysis*; D016172:DNA Fingerprinting; D005260:Female; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats; D008875:Middle Aged; D012737:Sex Factors; D014110:Touch; D055815:Young Adult
chemical_list: D004247:DNA
keywords: Age; DNA; Forensics; Touch DNA; Transfer; Unwashed hands