title: Isolation and identification of metabolites of bakuchiol in rats.
abstract: Bakuchiol, the main active component of Psoralea corylifolia, showed a range of significant pharmacological activities, including antimicrobial, antiinflammatory, reduction of bone loss and estrogenic activities. In this research, 12 metabolites, including 11 new compounds, were isolated from the urine and feces of rats after oral administration of bakuchiol, and their structures were elucidated by extensive spectroscopic analysis. The possible metabolic pathways of bakuchiol in rats were proposed, and a rare bile acid conjugation reaction was found. In addition, bakuchiol and its metabolites M1-M3 were studied for their alkaline phosphatase (ALP) activities on MC3T3-E1 cells and cytotoxicity on HKC-8 cells. The data showed that bakuchiol exerted significant effects on ALP activity of MC3T3-E1 cells and cytotoxicity on HKC-8 cells, while M1-M3 only showed ALP activities at 10(-5)M on MC3T3-E1 cells and no cytotoxicity on HKC-8 cells.
authors.affiliation: College of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China.
authors.forename: Pei-le
authors.initials: PL
authors.lastname: Wang
authors.affiliation: Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy, Jinan University, Guangzhou 510632, China.
authors.forename: Feng-xiang
authors.initials: FX
authors.lastname: Zhang
authors.affiliation: Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy, Jinan University, Guangzhou 510632, China; Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Kowloon, Hong Kong, China.
authors.forename: Zuo-cheng
authors.initials: ZC
authors.lastname: Qiu
authors.affiliation: Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy, Jinan University, Guangzhou 510632, China.
authors.forename: Zhi-hong
authors.initials: ZH
authors.lastname: Yao
authors.affiliation: Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Kowloon, Hong Kong, China.
authors.forename: Man-sau
authors.initials: MS
authors.lastname: Wong
authors.affiliation: College of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China; Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy, Jinan University, Guangzhou 510632, China. Electronic address: tyaoxs@jnu.edu.cn.
authors.forename: Xin-sheng
authors.initials: XS
authors.lastname: Yao
authors.affiliation: Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy, Jinan University, Guangzhou 510632, China. Electronic address: daiyi1004@163.com.
authors.forename: Yi
authors.initials: Y
authors.lastname: Dai
mesh_terms: D016475:3T3 Cells; D000818:Animals; D002460:Cell Line; D005243:Feces / Q000737:chemistry; D006801:Humans; D008297:Male; D051379:Mice; D015394:Molecular Structure; D010636:Phenols / Q000378:metabolism / Q000493:pharmacokinetics*; D029907:Psoralea / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014556:Urine / Q000737:chemistry
chemical_list: D010636:Phenols; C012765:bakuchiol
keywords: Bakuchiol; HKC-8 cytotoxicity; MC3T3-E1 cell ALP activity; Metabolites; Psoralea corylifolia