title: The Selective Myosin II Inhibitor Blebbistatin Reversibly Eliminates Gastrovascular Flow and Stolon Tip Pulsations in the Colonial Hydroid Podocoryna carnea.
abstract: Blebbistatin reversibly disrupted both stolon tip pulsations and gastrovascular flow in the colonial hydroid Podocoryna carnea. Epithelial longitudinal muscles of polyps were unaffected by blebbistatin, as polyps contracted when challenged with a pulse of KCl. Latrunculin B, which sequesters G actin preventing F actin assembly, caused stolons to retract, exposing focal adhesions where the tip epithelial cells adhere to the substratum. These results are consistent with earlier suggestions that non-muscle myosin II provides the motive force for stolon tip pulsations and further suggest that tip oscillations are functionally coupled to hydrorhizal axial muscle contraction.
authors.affiliation: Department of Ecology and Evolutionary Biology, Yale University, New Haven, Connecticut, United States of America.
authors.forename: Noah
authors.initials: N
authors.lastname: Connally
authors.affiliation: Department of Ecology and Evolutionary Biology, Yale University, New Haven, Connecticut, United States of America.
authors.forename: Christopher P
authors.initials: CP
authors.lastname: Anderson
authors.affiliation: 20 Colony Road, New Haven, Connecticut, United States of America.
authors.forename: Jules E
authors.initials: JE
authors.lastname: Bolton
authors.affiliation: Department of Geology and Geophysics, Yale University, New Haven, Connecticut, United States of America.
authors.forename: Edward W
authors.initials: EW
authors.lastname: Bolton
authors.affiliation: Department of Ecology and Evolutionary Biology, Yale University, New Haven, Connecticut, United States of America.|Smithsonian Marine Station, Fort Pierce, Florida, United States of America.
authors.forename: Leo W
authors.initials: LW
authors.lastname: Buss
mesh_terms: D000818:Animals; D006576:Heterocyclic Compounds, 4 or More Rings / Q000494:pharmacology*; D037402:Hydrozoa / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D009119:Muscle Contraction / Q000187:drug effects; D024462:Myosin Type II / Q000037:antagonists & inhibitors*; D059008:Time-Lapse Imaging
chemical_list: D006576:Heterocyclic Compounds, 4 or More Rings; C472645:blebbistatin; D024462:Myosin Type II