title: Optimization of a QuEChERS based method by means of central composite design for pesticide multiresidue determination in orange juice by UHPLC-MS/MS.
abstract: In this study, different extraction procedures based on the QuEChERS method were compared for the multiresidue determination of pesticides in orange juice by ultra high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS). After choosing preliminary conditions, an experimental design was carried out with the variables C18, PSA, NaOH and CH3COONa to optimize the sample preparation step. The validation results of the validation were satisfactory, since the method presented recoveries between 70% and 118%, with RSD lower than 19% for spike levels between 10 and 100 μg L(-1). The method limit of detection (LOD) and limit of quantification (LOQ) ranged from 3.0 to 7.6 μg L(-1) and from 4.9 to 26 μg L(-1), respectively. The method developed was adequate for the determination of 74 pesticide residues in orange juice.
authors.affiliation: Laboratório de Análise de Resíduos de Pesticidas (LARP), Departamento de Química, Universidade Federal de Santa Maria, CEP 97105-900 Santa Maria, RS, Brazil.
authors.forename: Tiele M
authors.initials: TM
authors.lastname: Rizzetti
authors.affiliation: Laboratório de Análise de Resíduos de Pesticidas (LARP), Departamento de Química, Universidade Federal de Santa Maria, CEP 97105-900 Santa Maria, RS, Brazil.
authors.forename: Magali
authors.initials: M
authors.lastname: Kemmerich
authors.affiliation: Laboratório de Análise de Resíduos de Pesticidas (LARP), Departamento de Química, Universidade Federal de Santa Maria, CEP 97105-900 Santa Maria, RS, Brazil.
authors.forename: Manoel L
authors.initials: ML
authors.lastname: Martins
authors.affiliation: Laboratório de Análise de Resíduos de Pesticidas (LARP), Departamento de Química, Universidade Federal de Santa Maria, CEP 97105-900 Santa Maria, RS, Brazil.
authors.forename: Osmar D
authors.initials: OD
authors.lastname: Prestes
authors.affiliation: Laboratório de Análise de Resíduos de Pesticidas (LARP), Departamento de Química, Universidade Federal de Santa Maria, CEP 97105-900 Santa Maria, RS, Brazil.
authors.forename: Martha B
authors.initials: MB
authors.lastname: Adaime
authors.affiliation: Laboratório de Análise de Resíduos de Pesticidas (LARP), Departamento de Química, Universidade Federal de Santa Maria, CEP 97105-900 Santa Maria, RS, Brazil. Electronic address: rzanella@base.ufsm.br.
authors.forename: Renato
authors.initials: R
authors.lastname: Zanella
mesh_terms: D001628:Beverages / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D032084:Citrus sinensis / Q000737:chemistry*; D010573:Pesticide Residues / Q000032:analysis / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D010573:Pesticide Residues
keywords: Central composite design; Orange juice; Pesticides; QuEChERS; UHPLC–MS/MS