title: ESRRB polymorphisms are associated with comorbidity of temporomandibular disorders and rotator cuff disease.
abstract: Temporomandibular disorders (TMD) are associated with comorbidity. Shoulder pain is among the symptoms associated with TMD. The purpose of this study was to investigate the association between TMD and rotator cuff disease (RCD) and related genetic aspects. All subjects underwent orofacial and shoulder examinations. The control group comprised 30 subjects with no pain. Affected subjects were divided into three groups: RCD (TMD-free, n=16), TMD (RCD-free, n=13), and TMD/RCD (patients with both RCD and TMD, n=49). A total of eight single nucleotide polymorphisms in the ESRRB gene were investigated. A chemiluminescent immunoassay was used to measure estradiol levels. Surface electromyography recorded head and cervical muscle activity. The χ(2) test and Student t-test/Mann-Whitney test were used to assess the significance of nominal and continuous variables. A P-value of <0.05 was considered significant. TMD subjects were seven times more susceptible to RCD than controls. The rs1676303 TT (P=0.02) and rs6574293 GG (P=0.04) genotypes were associated with RCD and TMD, respectively. TMD/RCD subjects showed associations with rs4903399 (P=0.02), rs10132091 (P=0.02), and CTTCTTAG/CCTCTCAG (P=0.01) haplotypes and lower muscle activity. Estradiol levels were similar among groups. This study supports TMD as a risk factor for RCD. ESRRB haplotypes and low muscle activity are common biomechanical characteristics in subjects with both diseases.
authors.affiliation: School of Dentistry and Clinical Research Unit, Fluminense Federal University, Niterói, RJ, Brazil.
authors.forename: L L
authors.initials: LL
authors.lastname: Bonato
authors.affiliation: School of Dentistry and Clinical Research Unit, Fluminense Federal University, Niterói, RJ, Brazil.
authors.forename: V
authors.initials: V
authors.lastname: Quinelato
authors.affiliation: Dental Clinical Department and Oral Implantology Post-graduation, Fluminense Federal University, Niterói, RJ, Brazil.
authors.forename: A da R
authors.initials: Ada R
authors.lastname: Pinheiro
authors.affiliation: Department of Orthopedic Surgery, Center of Shoulder and Elbow Surgery, National Institute of Traumatology and Orthopedics, Rio de Janeiro, RJ, Brazil.
authors.forename: M V G
authors.initials: MV
authors.lastname: Amaral
authors.affiliation: School of Dentistry and Clinical Research Unit, Fluminense Federal University, Niterói, RJ, Brazil.
authors.forename: F N
authors.initials: FN
authors.lastname: de Souza
authors.affiliation: Clinical Research Unit and Biology Institute, Fluminense Federal University, Niterói, RJ, Brazil.
authors.forename: J C
authors.initials: JC
authors.lastname: Lobo
authors.affiliation: Research Division, National Institute of Traumatology and Orthopedics, Rio de Janeiro, RJ, Brazil.
authors.forename: D P
authors.initials: DP
authors.lastname: Aguiar
authors.affiliation: Research Division, National Institute of Traumatology and Orthopedics, Rio de Janeiro, RJ, Brazil.
authors.forename: L M M
authors.initials: LM
authors.lastname: Augusto
authors.affiliation: Department of Oral Biology, School of Dental Medicine, University of Pittsburgh, Pittsburgh, USA.
authors.forename: A R
authors.initials: AR
authors.lastname: Vieira
authors.affiliation: Neuromuscular Research Laboratory, National Institute of Traumatology and Orthopedics, Rio de Janeiro, RJ, Brazil.
authors.forename: J I
authors.initials: JI
authors.lastname: Salles
authors.affiliation: Neuromuscular Research Laboratory, National Institute of Traumatology and Orthopedics, Rio de Janeiro, RJ, Brazil.
authors.forename: V R A
authors.initials: VR
authors.lastname: Cossich
authors.affiliation: Department of Trauma and Orthopedic Surgery, National Institute of Traumatology and Orthopedics, Rio de Janeiro, RJ, Brazil.
authors.forename: J A M
authors.initials: JA
authors.lastname: Guimarães
authors.affiliation: Department of Prosthodontics, Fluminense Federal University, Niterói, RJ, Brazil.
authors.forename: C V D
authors.initials: CV
authors.lastname: de Gouvêa
authors.affiliation: National Institute of Metrology, Quality and Technology, Rio de Janeiro, RJ, Brazil; Cell Therapy Center, Clinical Research Unit and Biology Institute, Fluminense Federal University, Niterói, RJ, Brazil.
authors.forename: J M
authors.initials: JM
authors.lastname: Granjeiro
authors.affiliation: School of Dentistry and Clinical Research Unit, Fluminense Federal University, Niterói, RJ, Brazil. Electronic address: plcasado@hotmail.com.
authors.forename: P L
authors.initials: PL
authors.lastname: Casado
mesh_terms: D001938:Brazil; D015897:Comorbidity; D003430:Cross-Sectional Studies; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009135:Muscular Diseases / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D011960:Receptors, Estrogen / Q000235:genetics*; D017006:Rotator Cuff; D013705:Temporomandibular Joint Disorders / Q000235:genetics*
chemical_list: C490918:ESRRB protein, human; D011960:Receptors, Estrogen
keywords: disease susceptibility; estradiol; polymorphism; rotator cuff disease; temporomandibular disorder