title: [Secondary metabolites from a deep-sea-derived actinomycete Micrococcus sp. R21].
abstract: To investigate cytotoxic secondary metabolites of Micrococcus sp. R21, an actinomycete isolated from a deep-sea sediment (-6 310 m; 142 degrees 19. 9' E, 10 degrees 54. 6' N) of the Western Pacific Ocean, column chromatography was introduced over silica gel, ODS, and Sephadex LH-20. As a result, eight compounds were obtained. By mainly detailed analysis of the NMR data, their structures were elucidated as cyclo(4-hydroxy-L-Pro-L-leu) (1), cyclo(L-Pro-L-Gly) (2), cyclo( L-Pro-L-Ala) (3), cyclo( D-Pro-L-Leu) (4), N-β-acetyltryptamine (5), 2-hydroxybenzoic acid (6), and phenylacetic acid (7). Compound 1 exhibited weak cytotoxic activity against RAW264. 7 cells with IC50 value of 9.1 μmol x L(-1).
authors.forename: Kun
authors.initials: K
authors.lastname: Peng
authors.forename: Rui-qiang
authors.initials: RQ
authors.lastname: Su
authors.forename: Gai-yun
authors.initials: GY
authors.lastname: Zhang
authors.forename: Xuan-xuan
authors.initials: XX
authors.lastname: Cheng
authors.forename: Quan
authors.initials: Q
authors.lastname: Yang
authors.forename: Yong-hong
authors.initials: YH
authors.lastname: Liu
authors.forename: Xian-wen
authors.initials: XW
authors.lastname: Yang
mesh_terms: D000818:Animals; D001685:Biological Factors / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D008264:Macrophages / Q000166:cytology / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D051379:Mice; D008837:Micrococcus / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D015394:Molecular Structure; D010802:Phylogeny; D000067996:RAW 264.7 Cells; D012623:Seawater / Q000382:microbiology*; D064210:Secondary Metabolism
chemical_list: D001685:Biological Factors