title: Construction, complete sequence, and annotation of a BAC contig covering the silkworm chorion locus.
abstract: The silkmoth chorion was studied extensively by F.C. Kafatos' group for almost 40 years. However, the complete structure of the chorion locus was not obtained in the genome sequence of Bombyx mori published in 2008 due to repetitive sequences, resulting in gaps and an incomplete view of the locus. To obtain the complete sequence of the chorion locus, expressed sequence tags (ESTs) derived from follicular epithelium cells were used as probes to screen a bacterial artificial chromosome (BAC) library. Seven BACs were selected to construct a contig which covered the whole chorion locus. By Sanger sequencing, we successfully obtained complete sequences of the chorion locus spanning 871,711 base pairs on chromosome 2, where we annotated 127 chorion genes. The dataset reported here will recruit more researchers to revisit one of the oldest model systems which has been used to study developmentally regulated gene expression. It also provides insights into egg development and fertilization mechanisms and is relevant to applications related to improvements in breeding procedures and transgenesis.
authors.affiliation: State Key Laboratory of Silkworm Genome Biology , Chongqing 400716, China.
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Kirin Brewery Co. Ltd , Toride 302-8502, Japan.
authors.forename: Junko
authors.initials: J
authors.lastname: Nohata
authors.affiliation: State Key Laboratory of Silkworm Genome Biology , Chongqing 400716, China.
authors.forename: Huizhen
authors.initials: H
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Silkworm Genome Biology , Chongqing 400716, China.
authors.forename: Shenglong
authors.initials: S
authors.lastname: Li
authors.affiliation: State Key Laboratory of Silkworm Genome Biology , Chongqing 400716, China.
authors.forename: Jianqiu
authors.initials: J
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Silkworm Genome Biology , Chongqing 400716, China.
authors.forename: Youbing
authors.initials: Y
authors.lastname: Guo
authors.affiliation: National Institute of Agrobiological Sciences , Tsukuba 305-8634, Japan.
authors.forename: Kimiko
authors.initials: K
authors.lastname: Yamamoto
authors.affiliation: National Institute of Agrobiological Sciences , Tsukuba 305-8634, Japan.
authors.forename: Keiko
authors.initials: K
authors.lastname: Kadono-Okuda
authors.affiliation: State Key Laboratory of Silkworm Genome Biology , Chongqing 400716, China.
authors.forename: Chun
authors.initials: C
authors.lastname: Liu
authors.affiliation: Centre for DNA Fingerprinting and Diagnostics , Hyderabad 500001, India.
authors.forename: Kallare P
authors.initials: KP
authors.lastname: Arunkumar
authors.affiliation: Centre for DNA Fingerprinting and Diagnostics , Hyderabad 500001, India.
authors.forename: Javaregowda
authors.initials: J
authors.lastname: Nagaraju
authors.affiliation: State Key Laboratory of Silkworm Genome Biology , Chongqing 400716, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Silkworm Genome Biology , Chongqing 400716, China.
authors.forename: Shiping
authors.initials: S
authors.lastname: Liu
authors.affiliation: Insect Molecular Genetics and Biotechnology, Institute of Biosciences & Applications, National Centre for Scientific Research 'Demokritos' , Athens 15310, Greece.
authors.forename: Vassiliki
authors.initials: V
authors.lastname: Labropoulou
authors.affiliation: Insect Molecular Genetics and Biotechnology, Institute of Biosciences & Applications, National Centre for Scientific Research 'Demokritos' , Athens 15310, Greece.
authors.forename: Luc
authors.initials: L
authors.lastname: Swevers
authors.affiliation: Insect Molecular Genetics and Biotechnology, Institute of Biosciences & Applications, National Centre for Scientific Research 'Demokritos' , Athens 15310, Greece.
authors.forename: Panagiota
authors.initials: P
authors.lastname: Tsitoura
authors.affiliation: Insect Molecular Genetics and Biotechnology, Institute of Biosciences & Applications, National Centre for Scientific Research 'Demokritos' , Athens 15310, Greece.
authors.forename: Kostas
authors.initials: K
authors.lastname: Iatrou
authors.affiliation: Indian Institute of Science , Bangalore 560012, India.
authors.forename: Karumathil P
authors.initials: KP
authors.lastname: Gopinathan
authors.affiliation: University of Rhode Island , Kingston, RI 02881, USA.
authors.forename: Marian R
authors.initials: MR
authors.lastname: Goldsmith
authors.affiliation: State Key Laboratory of Silkworm Genome Biology , Chongqing 400716, China.
authors.forename: Qingyou
authors.initials: Q
authors.lastname: Xia
authors.affiliation: State Key Laboratory of Silkworm Genome Biology , Chongqing 400716, China.
authors.forename: Kazuei
authors.initials: K
authors.lastname: Mita
mesh_terms: D000818:Animals; D012831:Bombyx / Q000196:embryology / Q000235:genetics*; D002823:Chorion; D002874:Chromosome Mapping; D022004:Chromosome Structures; D022202:Chromosomes, Artificial, Bacterial; D020224:Expressed Sequence Tags; D015723:Gene Library; D049750:Genome, Insect; D058977:Molecular Sequence Annotation
keywords: DNA sequencing; Molecular biology