title: Effect of Hypoxanthine on Functional Activity of Nucleoside Transporters ENT1 and ENT2 in Caco-2 Polar Epithelial Intestinal Cells.
abstract: We studied regulation of hypoxanthine transport depending on its concentration in the culture medium. Caco-2 cells were differentiated on membrane filters to create a model of the intestine. Different hypoxanthine uptake on the apical and basolateral cell membranes was observed. The expression of SLC29 family genes encoding passive nucleoside transporters increased upon changes in hypoxanthine concentration in the medium Localization of the transporters and their influence on the effect of pharmacological preparations are discussed.
authors.affiliation: BioClinicum Research-and-Production Center, Moscow, Russia. n.seniavina@bioclinicum.com.
authors.forename: N V
authors.initials: NV
authors.lastname: Senyavina
authors.affiliation: Moscow State Engineering University, Moscow, Russia.
authors.forename: S A
authors.initials: SA
authors.lastname: Tonevitskaya
mesh_terms: D001692:Biological Transport; D045744:Cell Line, Tumor; D003106:Colon / Q000473:pathology*; D003110:Colonic Neoplasms / Q000378:metabolism / Q000473:pathology; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism; D033721:Equilibrative Nucleoside Transporter 1 / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D033722:Equilibrative-Nucleoside Transporter 2 / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D019271:Hypoxanthine / Q000494:pharmacology*; D005810:Multigene Family; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology; D011687:Purines / Q000378:metabolism*; D011743:Pyrimidines / Q000378:metabolism*
chemical_list: D033721:Equilibrative Nucleoside Transporter 1; D033722:Equilibrative-Nucleoside Transporter 2; D009363:Neoplasm Proteins; D011687:Purines; D011743:Pyrimidines; C438649:SLC29A1 protein, human; C438654:SLC29A2 protein, human; D019271:Hypoxanthine
keywords: Caco-2; hypoxanthine, purine re-synthesis pathway; metabolism of purines; nucleoside transporters