title: Application of Green Tea Catechin for Inducing the Osteogenic Differentiation of Human Dedifferentiated Fat Cells in Vitro.
abstract: Despite advances in stem cell biology, there are few effective techniques to promote the osteogenic differentiation of human primary dedifferentiated fat (DFAT) cells. We attempted to investigate whether epigallocatechin-3-gallate (EGCG), the main component of green tea catechin, facilitates early osteogenic differentiation and mineralization on DFAT cells in vitro. DFAT cells were treated with EGCG (1.25-10 μM) in osteogenic medium (OM) with or without 100 nM dexamethasone (Dex) for 12 days (hereafter two osteogenic media were designated as OM(Dex) and OM). Supplementation of 1.25 μM EGCG to both the media effectively increased the mRNA expression of collagen 1 (COL1A1) and runt-related transcription factor 2 (RUNX2) and also increased proliferation and mineralization. Compared to OM(Dex) with EGCG, OM with EGCG induced earlier expression for COL1A1 and RUNX2 at day 1 and higher mineralization level at day 12. OM(Dex) with 10 μM EGCG remarkably hampered the proliferation of the DFAT cells. These results suggest that OM(without Dex) with EGCG might be a preferable medium to promote proliferation and to induce osteoblast differentiation of DFAT cells. Our findings provide an insight for the combinatory use of EGCG and DFAT cells for bone regeneration and stem cell-based therapy.
authors.affiliation: Department of Oral Implantology, Osaka Dental University, Osaka 573-1121, Japan. kaida-k@cc.osaka-dent.ac.jp.
authors.forename: Koji
authors.initials: K
authors.lastname: Kaida
authors.affiliation: Institute of Dental Research, Osaka Dental University, Osaka 573-1121, Japan. honda-y@cc.osaka-dent.ac.jp.
authors.forename: Yoshitomo
authors.initials: Y
authors.lastname: Honda
authors.affiliation: Department of Biomaterials, Osaka Dental University, Osaka 573-1121, Japan. yoshiya@cc.osaka-dent.ac.jp.
authors.forename: Yoshiya
authors.initials: Y
authors.lastname: Hashimoto
authors.affiliation: Department of Fixed Prosthodontics and Occlusion, Osaka Dental University, Osaka 573-1121, Japan. tanaka-m@cc.osaka-dent.ac.jp.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Tanaka
authors.affiliation: Department of Oral Implantology, Osaka Dental University, Osaka 573-1121, Japan. baba-s@cc.osaka-dent.ac.jp.
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Baba
mesh_terms: D017667:Adipocytes / Q000166:cytology* / Q000187:drug effects*; D000469:Alkaline Phosphatase / Q000378:metabolism; D015415:Biomarkers; D002113:Calcification, Physiologic / Q000187:drug effects; D002392:Catechin / Q000031:analogs & derivatives / Q000737:chemistry / Q000494:pharmacology*; D054337:Cell Dedifferentiation / Q000187:drug effects*; D049109:Cell Proliferation; D002478:Cells, Cultured; D015870:Gene Expression; D006801:Humans; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects* / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D013662:Tea / Q000737:chemistry*
chemical_list: D015415:Biomarkers; D012333:RNA, Messenger; D013662:Tea; D002392:Catechin; C045651:epigallocatechin gallate; D000469:Alkaline Phosphatase
keywords: EGCG; bone; catechin; dedifferentiated fat cell; osteoblast; polyphenol; stem cells