title: Tetrahydroxystilbene glucoside inhibits TNF-α-induced migration of vascular smooth muscle cells via suppression of vimentin.
abstract: Vascular smooth muscle cell (VSMC) migration triggered by TNF-α is an important event that occurs during the development of atherosclerosis. 2,3,5,4'-Tetrahydroxystilbene-2-O-β-d-glucoside (TSG) has been proven to exhibit significant anti-atherosclerotic activity. Herein we investigate the inhibitory effect of TSG on TNF-α-induced VSMC migration and explore the underlying mechanisms. TSG pretreatment markedly inhibited TNF-α-induced cell migration. The inhibition of vimentin redistribution and expression was involved in the inhibitory effect of TSG on VSMC migration. The suppression of vimentin expression by shRNA in VSMCs significantly inhibited TNF-α-induced cell migration. Furthermore, TSG inhibited the TNF-α-induced expression of TGFβ1 and TGFβR1, and phosphorylation of TGFβR1 and Smad2/3. TSG also suppressed the nuclear translocation of Smad4 induced by TNF-α. These results suggest that TSG inhibits VSMC migration induced by TNF-α through inhibiting vimentin rearrangement and expression. The interruption of TGFβ/Smad pathway appears to be responsible for the suppression of TSG on vimentin expression.
authors.affiliation: Department of Pharmacology, School of Pharmacy, Nantong University, 19 QiXiu Road, Nantong 226001, P. R. China.|Department of Pharmacology, School of Pharmacy, Nantong University, 19 QiXiu Road, Nantong 226001, P. R. China.
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Yao
authors.affiliation: Department of Pharmacology, School of Pharmacy, Nantong University, 19 QiXiu Road, Nantong 226001, P. R. China.|Department of Pharmacology, School of Pharmacy, Nantong University, 19 QiXiu Road, Nantong 226001, P. R. China.
authors.forename: Qinju
authors.initials: Q
authors.lastname: Sun
authors.affiliation: Department of Pharmacology, School of Pharmacy, Nantong University, 19 QiXiu Road, Nantong 226001, P. R. China.|Department of Pharmacology, School of Pharmacy, Nantong University, 19 QiXiu Road, Nantong 226001, P. R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Huang
authors.affiliation: Department of Pharmacology, School of Pharmacy, Nantong University, 19 QiXiu Road, Nantong 226001, P. R. China.|Department of Pharmacology, School of Pharmacy, Nantong University, 19 QiXiu Road, Nantong 226001, P. R. China.
authors.forename: Guoliang
authors.initials: G
authors.lastname: Meng
authors.affiliation: Department of Pharmacology, School of Pharmacy, Nantong University, 19 QiXiu Road, Nantong 226001, P. R. China.|Department of Pharmacology, School of Pharmacy, Nantong University, 19 QiXiu Road, Nantong 226001, P. R. China.
authors.forename: Huiming
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Pharmacology, School of Pharmacy, Nantong University, 19 QiXiu Road, Nantong 226001, P. R. China.|Department of Pharmacology, School of Pharmacy, Nantong University, 19 QiXiu Road, Nantong 226001, P. R. China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Jing
authors.affiliation: Department of Pharmacology, School of Pharmacy, Nantong University, 19 QiXiu Road, Nantong 226001, P. R. China.|Department of Pharmacology, School of Pharmacy, Nantong University, 19 QiXiu Road, Nantong 226001, P. R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
keywords: Tumor Necrosis Factor-α; cell migration; facteur nécrosant des tumeurs-α; migration cellulaire; tetrahydroxystilbene glucoside; tétrahydroxystilbène glucoside; vimentin; vimentine