title: The Mouse INO80 Chromatin-Remodeling Complex Is an Essential Meiotic Factor for Spermatogenesis.
abstract: The ability to faithfully transmit genetic information across generations via the germ cells is a critical aspect of mammalian reproduction. The process of germ cell development requires a number of large-scale modulations of chromatin within the nucleus. One such occasion arises during meiotic recombination, when hundreds of DNA double-strand breaks are induced and subsequently repaired, enabling the transfer of genetic information between homologous chromosomes. The inability to properly repair DNA damage is known to lead to an arrest in the developing germ cells and sterility within the animal. Chromatin-remodeling activity, and in particular the BRG1 subunit of the SWI/SNF complex, has been shown to be required for successful completion of meiosis. In contrast, remodeling complexes of the ISWI and CHD families are required for postmeiotic processes. Little is known regarding the contribution of the INO80 family of chromatin-remodeling complexes, which is a particularly interesting candidate due to its well described functions during DNA double-strand break repair. Here we show that INO80 is expressed in developing spermatocytes during the early stages of meiotic prophase I. Based on this information, we used a conditional allele to delete the INO80 core ATPase subunit, thereby eliminating INO80 chromatin-remodeling activity in this lineage. The loss of INO80 resulted in an arrest during meiosis associated with a failure to repair DNA damage during meiotic recombination.
authors.affiliation: Curriculum in Genetics and Molecular Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina Department of Genetics, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina.
authors.forename: Daniel W
authors.initials: DW
authors.lastname: Serber
authors.affiliation: Curriculum in Genetics and Molecular Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina Department of Genetics, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina.
authors.forename: John S
authors.initials: JS
authors.lastname: Runge
authors.affiliation: Department of Genetics, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina.
authors.forename: Debashish U
authors.initials: DU
authors.lastname: Menon
authors.affiliation: Curriculum in Genetics and Molecular Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina Department of Genetics, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina terry_magnuson@med.unc.edu.
authors.forename: Terry
authors.initials: T
authors.lastname: Magnuson
mesh_terms: D000074183:ATPases Associated with Diverse Cellular Activities; D000251:Adenosine Triphosphatases / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D000818:Animals; D002843:Chromatin / Q000235:genetics* / Q000502:physiology*; D042002:Chromatin Assembly and Disassembly; D023902:Chromosome Pairing / Q000235:genetics / Q000502:physiology; D053903:DNA Breaks, Double-Stranded; D004260:DNA Repair; D004268:DNA-Binding Proteins; D008297:Male; D008540:Meiosis / Q000235:genetics* / Q000502:physiology*; D051379:Mice; D018345:Mice, Knockout; D011995:Recombination, Genetic; D013090:Spermatocytes; D013091:Spermatogenesis / Q000235:genetics* / Q000502:physiology*; D013737:Testis / Q000166:cytology
chemical_list: D002843:Chromatin; D004268:DNA-Binding Proteins; D000251:Adenosine Triphosphatases; D000074183:ATPases Associated with Diverse Cellular Activities; C000603449:INO80 protein, mouse
keywords: INO80; chromatin remodeling; meiotic recombination; spermatogenesis