title: Optimized Protocol for Isolation of Multipotent Mesenchymal Stromal Cells from Human Umbilical Cord.
abstract: Extraembryonic tissues, in particular, umbilical cord stroma are promising sources of multipotent mesenchymal stromal cells for regenerative medicine. In recent years, methods for isolation of mesenchymal stromal cells from different compartments of the umbilical cords based on enzymatic disaggregation of the tissue or on tissue explants have been proposed. Here we propose a protocol of isolation of multipotent mesenchymal stromal cells from the whole umbilical cord that combines the advantages of each approach and ensures sufficient cell yield for further experimental and clinical applications. A combination of short-term incubation of tissue fragments on cold collagenase solution followed by their culturing in the form of explants significantly increased the yield of cells with high proliferative activity, typical pluripotent mesenchymal stromal cell phenotype, and preserved differentiation capacity.
authors.affiliation: Russian Cardiology Research-and-Production Complex, Ministry of Health of the Russian Federation, Moscow, Russia. romanov@cardio.ru.|CryoCenter Umbilical Cord Blood Bank, Moscow, Russia. romanov@cardio.ru.
authors.forename: Yu A
authors.initials: YA
authors.lastname: Romanov
authors.affiliation: Russian Cardiology Research-and-Production Complex, Ministry of Health of the Russian Federation, Moscow, Russia.|CryoCenter Umbilical Cord Blood Bank, Moscow, Russia.
authors.forename: E E
authors.initials: EE
authors.lastname: Balashova
authors.affiliation: V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: N E
authors.initials: NE
authors.lastname: Volgina
authors.affiliation: Russian Cardiology Research-and-Production Complex, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: N V
authors.initials: NV
authors.lastname: Kabaeva
authors.affiliation: CryoCenter Umbilical Cord Blood Bank, Moscow, Russia.
authors.forename: T N
authors.initials: TN
authors.lastname: Dugina
authors.affiliation: V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: G T
authors.initials: GT
authors.lastname: Sukhikh
mesh_terms: D050156:Adipogenesis; D002455:Cell Division; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D017364:Collagenases; D006801:Humans; D007231:Infant, Newborn; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010012:Osteogenesis; D010969:Plastics; D039904:Pluripotent Stem Cells / Q000166:cytology*; D013193:Stainless Steel; D017154:Stromal Cells / Q000166:cytology*; D014470:Umbilical Cord / Q000166:cytology*
chemical_list: D010969:Plastics; D013193:Stainless Steel; D017364:Collagenases
keywords: Differentiation; collagenase; explant; multipotent mesenchymal stromal cells; umbilical cord