title: Investigation of antibacterial activity of aspidin BB against Propionibacterium acnes.
abstract: In the present study, antibacterial activity of four kinds of phloroglucinol derivatives extracted from Dryopteris fragrans (L.) Schott against S. aureus, S. epidermidis and P. acnes has been tested. Aspidin BB exerted the strongest antibacterial activity with minimal inhibition concentration (MIC) values ranging from 7.81 to 15.63 μg/mL. The time-kill assay indicated that aspidin BB could kill P. acnes completely at 2 MIC (MBC) within 4 h. By using AFM, we demonstrated extensive cell surface alterations of aspidin BB-treated P. acnes. SDS-PAGE of supernatant proteins and lipid peroxidation results showed that aspidin BB dose-dependently affected membrane permeability of P. acnes. DNA damage and protein degradation of P. acnes were also verified. SDS-PAGE of precipitated proteins revealed possible targets of aspidin BB, i.e., heat shock proteins (26 kDa) and lipase (33 kDa) which could all cause inflammation. Aspidin BB also seriously increased the inhibition rate of lipase activity from 10.20 to 65.20 % to possibly inhibit the inflammation. In conclusions, the effective constituents of D. fragrans (L.) Schott to treat acne might be phloroglucinol derivatives including aspidin BB, aspidin PB, aspidinol and dryofragin. Among this, aspidin BB inhibited the growth of P. acnes by disrupting their membrane, DNA and proteins and finally leaded to the cell death. The obtained data highlighted the potential of using aspidin BB as an alternative treatment for acne vulgaris.
authors.affiliation: College of Basic Medical Science, Peking University Health Science Center, Beijing, 100083, People's Republic of China.
authors.forename: Chang
authors.initials: C
authors.lastname: Gao
authors.affiliation: Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Box 332, Hexing Road 26, Harbin, 150040, People's Republic of China.
authors.forename: Na
authors.initials: N
authors.lastname: Guo
authors.affiliation: Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Box 332, Hexing Road 26, Harbin, 150040, People's Republic of China.
authors.forename: Na
authors.initials: N
authors.lastname: Li
authors.affiliation: Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Box 332, Hexing Road 26, Harbin, 150040, People's Republic of China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Peng
authors.affiliation: Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Box 332, Hexing Road 26, Harbin, 150040, People's Republic of China.
authors.forename: Peng
authors.initials: P
authors.lastname: Wang
authors.affiliation: Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Box 332, Hexing Road 26, Harbin, 150040, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Box 332, Hexing Road 26, Harbin, 150040, People's Republic of China.
authors.forename: Meng
authors.initials: M
authors.lastname: Luo
authors.affiliation: Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Box 332, Hexing Road 26, Harbin, 150040, People's Republic of China. yujie_fu@163.com.
authors.forename: Yu-Jie
authors.initials: YJ
authors.lastname: Fu
mesh_terms: D000152:Acne Vulgaris / Q000188:drug therapy*; D000900:Anti-Bacterial Agents / Q000627:therapeutic use*; D001426:Bacterial Proteins / Q000378:metabolism; D002463:Cell Membrane Permeability / Q000187:drug effects; D053938:DNA Fragmentation / Q000187:drug effects; D032492:Dryopteris; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007249:Inflammation / Q000188:drug therapy; D008049:Lipase / Q000037:antagonists & inhibitors; D008826:Microbial Sensitivity Tests; D018625:Microscopy, Atomic Force; D010696:Phloroglucinol / Q000031:analogs & derivatives* / Q000627:therapeutic use; D010936:Plant Extracts / Q000627:therapeutic use; D011425:Propionibacterium acnes / Q000187:drug effects*; D059748:Proteolysis / Q000187:drug effects; D013211:Staphylococcus aureus / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D010936:Plant Extracts; D010696:Phloroglucinol; D008049:Lipase; C010302:aspidin BB
keywords: Antibacterial; Aspidin BB; Dryopteris fragrans, Dryopteriaceae; Lipase; Membrane