title: Quantitative evaluation of the transplanted lin(-) hematopoietic cell migration kinetics.
abstract: Stem cells take part in organogenesis, cell maturation and injury repair. The migration is necessary for each of these functions to occur. The aim of this study was to investigate the kinetics of transplanted hematopoietic lin(-) cell population (which consists mainly of the stem and progenitor cells) in BALB/c mouse contact hypersensitivity model and quantify the migration to the site of inflammation in the affected foot and other healthy organs. Quantitative analysis was carried out with the real-time polymerase chain reaction method. Spleen, kidney, bone marrow, lung, liver, damaged and healthy foot tissue samples at different time points were collected for analysis. The quantitative data normalization was performed according to the comparative quantification method. The analysis of foot samples shows the significant migration of transplanted cells to the recipient mice affected foot. The quantity was more than 1000 times higher, as compared with that of the untreated foot. Due to the inflammation, the number of donor origin cells migrating to the lungs, liver, spleen and bone marrow was found to be decreased. Our data shows that transplanted cells selectively migrated into the inflammation areas of the foot edema. Also, the inflammation caused a secondary migration in ectopic spleen of hematopoietic stem cell niches and re-homing from the spleen to the bone marrow took place.
authors.affiliation: State Research Institute Center for Innovative Medicine, Žygimantų str. 9, LT-01102 Vilnius, Lithuania; Center for Physical Sciences and Technology, A. Goštauto Str. 11, LT-01108 Vilnius, Lithuania. Electronic address: Vytautas.Kaseta@gmail.com.
authors.forename: Vytautas
authors.initials: V
authors.lastname: Kašėta
authors.affiliation: State Research Institute Center for Innovative Medicine, Žygimantų str. 9, LT-01102 Vilnius, Lithuania.
authors.forename: Aida
authors.initials: A
authors.lastname: Vaitkuvienė
authors.affiliation: State Research Institute Center for Innovative Medicine, Žygimantų str. 9, LT-01102 Vilnius, Lithuania.
authors.forename: Aušra
authors.initials: A
authors.lastname: Liubavičiūtė
authors.affiliation: Center for Physical Sciences and Technology, A. Goštauto Str. 11, LT-01108 Vilnius, Lithuania.
authors.forename: Rūta
authors.initials: R
authors.lastname: Maciulevičienė
authors.affiliation: Center for Physical Sciences and Technology, A. Goštauto Str. 11, LT-01108 Vilnius, Lithuania.
authors.forename: Arūnas
authors.initials: A
authors.lastname: Stirkė
authors.affiliation: State Research Institute Center for Innovative Medicine, Žygimantų str. 9, LT-01102 Vilnius, Lithuania.
authors.forename: Genė
authors.initials: G
authors.lastname: Biziulevičienė
mesh_terms: D000818:Animals; D000943:Antigens, Differentiation / Q000378:metabolism; D001853:Bone Marrow / Q000276:immunology*; D019070:Cell Lineage; D002465:Cell Movement; D003877:Dermatitis, Contact / Q000276:immunology*; D004195:Disease Models, Animal; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000276:immunology*
chemical_list: D000943:Antigens, Differentiation
keywords: Inflammation; Lin(−) hematopoietic cell; Migration; Mouse bone marrow