title: Nucleostemin promotes the proliferation of human glioma via Wnt/β-Catenin pathway.
abstract: Nucleostemin, nucleolar guanosine triphosphate (GTP)-binding protein 3, is a member of the MMR1/HSR1 GTP-binding protein family. The important roles of nucleostemin in self-renewal, cell cycle regulation, apoptosis, and cell proliferation of various cancer types as been shown. Nevertheless, its expression and potential functions in human glioma is still unclear. In the present study, we demonstrated that up-regulation of nucleostemin was tightly related to poor 5-year-survival ratios. In serum-starved and re-feeding models of U251 and U373MG, we observed the rising expression of nucleostemin and p-β-Catenin (p-Tyr645) were accompanied with cell proliferation markers (cyclin D1 and proliferating cell nuclear antigen (PCNA)). Employing nucleostemin-depletion models, we found down-regulated nucleostemin and p-β-Catenin. The flow cytometry analysis proved the weakened cell proliferation. Moreover, we detected the translocation of β-Catenin into the nucleus was impaired, meaning the inhibition of the Wnt/β-Catenin pathway. Taken together, we identified a positive correlation between up-regulation of nucleostemin and human glioma cell proliferation and that knocking-down nucleostemin alleviated glioma proliferation by reducing β-Catenin transportation into the nucleus. All results suggested that nucleostemin might accelerate human glioma proliferation via the Wnt/β-Catenin pathway.
authors.affiliation: Departments of Neurosurgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, People's Republic of China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Bao
authors.affiliation: Departments of Neurosurgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, People's Republic of China.
authors.forename: Yunfeng
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Departments of Neurosurgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, People's Republic of China.
authors.forename: Lixiang
authors.initials: L
authors.lastname: Yang
authors.affiliation: Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, People's Republic of China.
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
authors.affiliation: Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Medical College of Nantong University, Nantong, Jiangsu Province, People's Republic of China.
authors.forename: Lianxin
authors.initials: L
authors.lastname: Zhu
authors.affiliation: Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Medical College of Nantong University, Nantong, Jiangsu Province, People's Republic of China.
authors.forename: Na
authors.initials: N
authors.lastname: Ban
authors.affiliation: Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Medical College of Nantong University, Nantong, Jiangsu Province, People's Republic of China.
authors.forename: Shaochen
authors.initials: S
authors.lastname: Fan
authors.affiliation: Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Medical College of Nantong University, Nantong, Jiangsu Province, People's Republic of China.
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Chen
authors.affiliation: Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Medical College of Nantong University, Nantong, Jiangsu Province, People's Republic of China.
authors.forename: Jie
authors.initials: J
authors.lastname: Sun
authors.affiliation: Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Medical College of Nantong University, Nantong, Jiangsu Province, People's Republic of China.
authors.forename: Chaoyan
authors.initials: C
authors.lastname: Shen
authors.affiliation: Departments of Neurosurgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, People's Republic of China.
authors.forename: Gang
authors.initials: G
authors.lastname: Cui
mesh_terms: D000328:Adult; D001932:Brain Neoplasms / Q000201:enzymology*; D045744:Cell Line, Tumor; D005260:Female; D019204:GTP-Binding Proteins / Q000378:metabolism*; D005910:Glioma / Q000201:enzymology*; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D009687:Nuclear Proteins / Q000378:metabolism*; D015854:Up-Regulation; D060449:Wnt Signaling Pathway
chemical_list: C469949:GNL3 protein, human; D009687:Nuclear Proteins; D019204:GTP-Binding Proteins
keywords: Wnt/β-Catenin; human glioma; nucleostemin; p-β-Catenin; proliferation