title: Dispositions of enrofloxacin and its major metabolite ciprofloxacin in Thai swamp buffaloes.
abstract: Given the limited information available in this species, the aim of this study was to investigate the pharmacokinetic characteristics of enrofloxacin (ER) and its major metabolite ciprofloxacin (CP) in buffaloes, Bubalus bubalis. ER was administered intravenously (i.v.) or subcutaneously (s.c.) to buffaloes at doses of 5.0 and 7.5 mg/kg BW, and plasma, urine and fecal samples were collected until 48 hr post-administration. The concentrations of ER and CP in the plasma, urine and feces were analyzed using high-performance liquid chromatography equipped with a fluorescence detector. The plasma concentrations of ER and CP could be determined up to 24 hr and 32 hr after i.v. and s.c. administrations at doses of 5.0 and 7.5 mg/kg BW, respectively. CP concentrations were always lower than those of parental drug. The s.c. bioavailability of ER was 52.36 ± 4.24% and 72.12 ± 5.39% at doses of 5.0 and 7.5 mg/kg BW, respectively. Both ER and CP were detectable in urine and feces up to 24 hr. ER and CP were mainly excreted via the urine. Based on the pharmacokinetic data and PK-PD indices, s.c. administration of ER at doses of 5.0 and 7.5 mg/kg BW might be appropriate for the treatment of susceptible bacterial diseases in Thai swamp buffaloes.
authors.affiliation: Department of Pharmacology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand.
authors.forename: Nitwarat
authors.initials: N
authors.lastname: Ruennarong
authors.forename: Kannika
authors.initials: K
authors.lastname: Wongpanit
authors.forename: Chainarong
authors.initials: C
authors.lastname: Sakulthaew
authors.forename: Mario
authors.initials: M
authors.lastname: Giorgi
authors.forename: Susumu
authors.initials: S
authors.lastname: Kumagai
authors.forename: Amnart
authors.initials: A
authors.lastname: Poapolathep
authors.forename: Saranya
authors.initials: S
authors.lastname: Poapolathep
mesh_terms: D061605:Administration, Intravenous; D000818:Animals; D000900:Anti-Bacterial Agents / Q000493:pharmacokinetics*; D002020:Buffaloes / Q000378:metabolism*; D002939:Ciprofloxacin / Q000008:administration & dosage / Q000493:pharmacokinetics*; D000077422:Enrofloxacin; D005260:Female; D024841:Fluoroquinolones / Q000008:administration & dosage / Q000493:pharmacokinetics*; D055103:Hypodermoclysis; D013785:Thailand
chemical_list: D000900:Anti-Bacterial Agents; D024841:Fluoroquinolones; D000077422:Enrofloxacin; D002939:Ciprofloxacin