title: Changes in the nuclear proteome of developing wheat (Triticum aestivum L.) grain.
abstract: Wheat grain end-use value is determined by complex molecular interactions that occur during grain development, including those in the cell nucleus. However, our knowledge of how the nuclear proteome changes during grain development is limited. Here, we analyzed nuclear proteins of developing wheat grains collected during the cellularization, effective grain-filling, and maturation phases of development, respectively. Nuclear proteins were extracted and separated by two-dimensional gel electrophoresis. Image analysis revealed 371 and 299 reproducible spots in gels with first dimension separation along pH 4-7 and pH 6-11 isoelectric gradients, respectively. The relative abundance of 464 (67%) protein spots changed during grain development. Abundance profiles of these proteins clustered in six groups associated with the major phases and phase transitions of grain development. Using nano liquid chromatography-tandem mass spectrometry to analyse 387 variant and non-variant protein spots, 114 different proteins were identified that were classified into 16 functional classes. We noted that some proteins involved in the regulation of transcription, like HMG1/2-like protein and histone deacetylase HDAC2, were most abundant before the phase transition from cellularization to grain-filling, suggesting that major transcriptional changes occur during this key developmental phase. The maturation period was characterized by high relative abundance of proteins involved in ribosome biogenesis. Data are available via ProteomeXchange with identifier PXD002999.
authors.affiliation: UMR1095 Genetics, Diversity and Ecophysiology of Cereals, Institut National de la Recherche Agronomique Clermont-Ferrand, France ; UMR1095 Genetics, Diversity and Ecophysiology of Cereals, Blaise Pascal University Aubière, France.
authors.forename: Titouan
authors.initials: T
authors.lastname: Bonnot
authors.affiliation: UMR1095 Genetics, Diversity and Ecophysiology of Cereals, Institut National de la Recherche Agronomique Clermont-Ferrand, France ; UMR1095 Genetics, Diversity and Ecophysiology of Cereals, Blaise Pascal University Aubière, France.
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Bancel
authors.affiliation: Metabolism Exploration Platform Proteomic Component, Institut National de la Recherche Agronomique Saint-Genès Champanelle, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Chambon
authors.affiliation: UMR1095 Genetics, Diversity and Ecophysiology of Cereals, Institut National de la Recherche Agronomique Clermont-Ferrand, France ; UMR1095 Genetics, Diversity and Ecophysiology of Cereals, Blaise Pascal University Aubière, France.
authors.forename: Julie
authors.initials: J
authors.lastname: Boudet
authors.affiliation: UMR1095 Genetics, Diversity and Ecophysiology of Cereals, Institut National de la Recherche Agronomique Clermont-Ferrand, France ; UMR1095 Genetics, Diversity and Ecophysiology of Cereals, Blaise Pascal University Aubière, France.
authors.forename: Gérard
authors.initials: G
authors.lastname: Branlard
authors.affiliation: UMR1095 Genetics, Diversity and Ecophysiology of Cereals, Institut National de la Recherche Agronomique Clermont-Ferrand, France ; UMR1095 Genetics, Diversity and Ecophysiology of Cereals, Blaise Pascal University Aubière, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Martre
keywords: 2D gel electrophoresis; LC-MS/MS; developing grain; nuclear proteins; wheat