title: Current and future resources for functional metagenomics.
abstract: Functional metagenomics is a powerful experimental approach for studying gene function, starting from the extracted DNA of mixed microbial populations. A functional approach relies on the construction and screening of metagenomic libraries-physical libraries that contain DNA cloned from environmental metagenomes. The information obtained from functional metagenomics can help in future annotations of gene function and serve as a complement to sequence-based metagenomics. In this Perspective, we begin by summarizing the technical challenges of constructing metagenomic libraries and emphasize their value as resources. We then discuss libraries constructed using the popular cloning vector, pCC1FOS, and highlight the strengths and shortcomings of this system, alongside possible strategies to maximize existing pCC1FOS-based libraries by screening in diverse hosts. Finally, we discuss the known bias of libraries constructed from human gut and marine water samples, present results that suggest bias may also occur for soil libraries, and consider factors that bias metagenomic libraries in general. We anticipate that discussion of current resources and limitations will advance tools and technologies for functional metagenomics research.
authors.affiliation: Department of Biology, University of Waterloo Waterloo, ON, Canada.
authors.forename: Kathy N
authors.initials: KN
authors.lastname: Lam
authors.affiliation: Department of Biology, University of Waterloo Waterloo, ON, Canada.
authors.forename: Jiujun
authors.initials: J
authors.lastname: Cheng
authors.affiliation: Department of Biology, University of Waterloo Waterloo, ON, Canada.
authors.forename: Katja
authors.initials: K
authors.lastname: Engel
authors.affiliation: Department of Biology, University of Waterloo Waterloo, ON, Canada.
authors.forename: Josh D
authors.initials: JD
authors.lastname: Neufeld
authors.affiliation: Department of Biology, University of Waterloo Waterloo, ON, Canada.
authors.forename: Trevor C
authors.initials: TC
authors.lastname: Charles
keywords: RK2; cloning bias; cosmid library; fosmid library; functional metagenomics; library bias; metagenomic library; pCC1FOS