title: Identification of 14-3-3 Family in Common Bean and Their Response to Abiotic Stress.
abstract: 14-3-3s are a class of conserved regulatory proteins ubiquitously found in eukaryotes, which play important roles in a variety of cellular processes including response to diverse stresses. Although much has been learned about 14-3-3s in several plant species, it remains unknown in common bean. In this study, 9 common bean 14-3-3s (PvGF14s) were identified by exhaustive data mining against the publicly available common bean genomic database. A phylogenetic analysis revealed that each predicted PvGF14 was clustered with two GmSGF14 paralogs from soybean. Both epsilon-like and non-epsilon classes of PvGF14s were found in common bean, and the PvGF14s belonging to each class exhibited similar gene structure. Among 9 PvGF14s, only 8 are transcribed in common bean. Expression patterns of PvGF14s varied depending on tissue type, developmental stage and exposure of plants to stress. A protein-protein interaction study revealed that PvGF14a forms dimer with itself and with other PvGF14 isoforms. This study provides a first comprehensive look at common bean 14-3-3 proteins, a family of proteins with diverse functions in many cellular processes, especially in response to stresses.
authors.affiliation: College of Plant Science, Jilin University, Changchun, China.
authors.forename: Ruihua
authors.initials: R
authors.lastname: Li
authors.affiliation: College of Plant Science, Jilin University, Changchun, China.
authors.forename: Xiaotong
authors.initials: X
authors.lastname: Jiang
authors.affiliation: College of Plant Science, Jilin University, Changchun, China.
authors.forename: Donghao
authors.initials: D
authors.lastname: Jin
authors.affiliation: Agriculture and Agri-Food Canada, Southern Crop Protection and Food Research Centre, London, Ontario, Canada.
authors.forename: Sangeeta
authors.initials: S
authors.lastname: Dhaubhadel
authors.affiliation: College of Plant Science, Jilin University, Changchun, China.
authors.forename: Shaomin
authors.initials: S
authors.lastname: Bian
authors.affiliation: College of Plant Science, Jilin University, Changchun, China.
authors.forename: Xuyan
authors.initials: X
authors.lastname: Li
mesh_terms: D048948:14-3-3 Proteins / Q000502:physiology*; D000595:Amino Acid Sequence; D002874:Chromosome Mapping; D016208:Databases, Factual; D020440:Gene Duplication; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D018745:Genome, Plant; D023281:Genomics; D008969:Molecular Sequence Data; D005810:Multigene Family; D010802:Phylogeny; D025941:Protein Interaction Mapping; D017386:Sequence Homology, Amino Acid; D013025:Glycine max / Q000235:genetics*; D013045:Species Specificity; D013312:Stress, Physiological; D013696:Temperature; D014018:Tissue Distribution; D020798:Two-Hybrid System Techniques
chemical_list: D048948:14-3-3 Proteins