title: An insight into the isolation, enumeration, and molecular detection of Listeria monocytogenes in food.
abstract: Listeria monocytogenes, a foodborne pathogen that can cause listeriosis through the consumption of food contaminated with this pathogen. The ability of L. monocytogenes to survive in extreme conditions and cause food contaminations have become a major concern. Hence, routine microbiological food testing is necessary to prevent food contamination and outbreaks of foodborne illness. This review provides insight into the methods for cultural detection, enumeration, and molecular identification of L. monocytogenes in various food samples. There are a number of enrichment and plating media that can be used for the isolation of L. monocytogenes from food samples. Enrichment media such as buffered Listeria enrichment broth, Fraser broth, and University of Vermont Medium (UVM) Listeria enrichment broth are recommended by regulatory agencies such as Food and Drug Administration-bacteriological and analytical method (FDA-BAM), US Department of Agriculture-Food and Safety (USDA-FSIS), and International Organization for Standardization (ISO). Many plating media are available for the isolation of L. monocytogenes, for instance, polymyxin acriflavin lithium-chloride ceftazidime aesculin mannitol, Oxford, and other chromogenic media. Besides, reference methods like FDA-BAM, ISO 11290 method, and USDA-FSIS method are usually applied for the cultural detection or enumeration of L. monocytogenes. most probable number technique is applied for the enumeration of L. monocytogenes in the case of low level contamination. Molecular methods including polymerase chain reaction, multiplex polymerase chain reaction, real-time/quantitative polymerase chain reaction, nucleic acid sequence-based amplification, loop-mediated isothermal amplification, DNA microarray, and next generation sequencing technology for the detection and identification of L. monocytogenes are discussed in this review. Overall, molecular methods are rapid, sensitive, specific, time- and labor-saving. In future, there are chances for the development of new techniques for the detection and identification of foodborne with improved features.
authors.affiliation: Biomedical Research Laboratory, Jeffrey Cheah School of Medicine and Health Sciences, Monash University Bandar Sunway, Malaysia.
authors.forename: Jodi Woan-Fei
authors.initials: JW
authors.lastname: Law
authors.affiliation: UKM Medical Molecular Biology Institute, UKM Medical Centre Kuala Lumpur, Malaysia.
authors.forename: Nurul-Syakima
authors.initials: NS
authors.lastname: Ab Mutalib
authors.affiliation: Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya Kuala Lumpur, Malaysia.
authors.forename: Kok-Gan
authors.initials: KG
authors.lastname: Chan
authors.affiliation: Biomedical Research Laboratory, Jeffrey Cheah School of Medicine and Health Sciences, Monash University Bandar Sunway, Malaysia.
authors.forename: Learn-Han
authors.initials: LH
authors.lastname: Lee
keywords: Listeria species; enumeration; foodborne pathogens; isolation; molecular detection