title: 3D Tumor Models and Time-Lapse Analysis by Multidimensional Microscopy.
abstract: The 3D culture is advantageous in reflecting the in vivo condition compared to the 2D culture; however, imaging 3D-cultured cells may be a challenge due to technical restrictions. Recent development of confocal spinning disc microscope system as well as sophisticated software has enabled us to monitor dynamism of cell movement in multiple dimensions. Here we describe the method for time-lapse imaging of 3D-cultured cancer cells co-cultured with non-cancerous cells and discuss current limitations and future perspectives.
authors.affiliation: Conway Institute, University College Dublin, Dublin, Ireland.
authors.forename: Dimitri
authors.initials: D
authors.lastname: Scholz
authors.affiliation: School of Medicine and Medical Science, University College Dublin, Dublin, Ireland. nobue.itasaki@bristol.ac.uk.|Faculty of Health Sciences, University of Bristol, Bristol, BS2 8EJ, UK. nobue.itasaki@bristol.ac.uk.
authors.forename: Nobue
authors.initials: N
authors.lastname: Itasaki
mesh_terms: D045744:Cell Line, Tumor; D002470:Cell Survival; D018920:Coculture Techniques / Q000379:methods*; D006801:Humans; D018613:Microscopy, Confocal / Q000379:methods*; D013997:Time Factors
keywords: Deconvolution; Live cell imaging; Multidimensional microscopy; Three-dimensional culture; Time-lapse imaging