title: Comparison of biological characteristics of mesenchymal stem cells derived from maternal-origin placenta and Wharton's jelly.
abstract: BACKGROUND
  Although mesenchymal stem cells (MSCs) from different sources share many similar characteristics, they also exhibit individual properties. In this study, we compared MSCs derived from Wharton's jelly in the umbilical cord with those derived from the decidual basalis in the maternal part of the placenta to better understand the similarities and differences between these two cell types.
  
  
  METHODS
  The morphology, immunophenotype (as assessed using flow cytometry), and multi-lineage differentiation potential were analyzed. Karyotype analysis was carried out to determine the origin of the MSCs. Growth kinetics were evaluated using analysis of the population doubling time and cell cycle. Immunosuppressive function was analyzed using mixed lymphocyte culture.
  
  
  RESULTS
  MSCs from Wharton's jelly and the decidua basalis exhibited similar morphology, immunophenotype, and differentiation potential to osteogenesis and adipogenesis. The percentage of MSCs in the G0/G1 phase was higher in the case of Wharton's jelly than in the case of the decidua basalis (P < 0.05). Decidual MSCs displayed more remarkable immunosuppressive effects on phytohemagglutinin-stimulated T-cell proliferation (P < 0.05).
  
  
  CONCLUSIONS
  MSCs from both sources had similar basic biological properties, but decidual MSCs had slower proliferation and stronger immunosuppressive function.
authors.affiliation: Department of Cardiology, Taizhou Renmin Hospital, Taizhou, Jiangsu Province, China. chengecai@163.com.
authors.forename: Gecai
authors.initials: G
authors.lastname: Chen
authors.affiliation: Stem Cell Research Center, Taizhou, Jiangsu Province, China. yueaihuan@163.com.
authors.forename: Aihuan
authors.initials: A
authors.lastname: Yue
authors.affiliation: Department of Cardiology, Taizhou Renmin Hospital, Taizhou, Jiangsu Province, China. ruanzhongbao2008@163.com.
authors.forename: Zhongbao
authors.initials: Z
authors.lastname: Ruan
authors.affiliation: Department of Cardiology, Taizhou Renmin Hospital, Taizhou, Jiangsu Province, China. yinyigang2015@sina.com.
authors.forename: Yigang
authors.initials: Y
authors.lastname: Yin
authors.affiliation: Department of Cardiology, Taizhou Renmin Hospital, Taizhou, Jiangsu Province, China. wangruzhu2014@163.com.
authors.forename: Ruzhu
authors.initials: R
authors.lastname: Wang
authors.affiliation: Department of Cardiology, Taizhou Renmin Hospital, Taizhou, Jiangsu Province, China. renyin2006@163.com.
authors.forename: Yin
authors.initials: Y
authors.lastname: Ren
authors.affiliation: Department of Cardiology, Taizhou Renmin Hospital, Taizhou, Jiangsu Province, China. yueaihuan@beike.cc.
authors.forename: Li
authors.initials: L
authors.lastname: Zhu
mesh_terms: D050156:Adipogenesis; D002453:Cell Cycle; D002454:Cell Differentiation; D049109:Cell Proliferation; D002469:Cell Separation; D003656:Decidua / Q000166:cytology / Q000276:immunology; D005260:Female; D006801:Humans; D007108:Immune Tolerance; D016130:Immunophenotyping; D007231:Infant, Newborn; D007621:Karyotyping; D007959:Lymphocyte Culture Test, Mixed; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000276:immunology; D010012:Osteogenesis; D010920:Placenta / Q000166:cytology* / Q000276:immunology; D011247:Pregnancy; D059631:Wharton Jelly / Q000166:cytology* / Q000276:immunology