title: Integrating mass spectrometry and genomics for cyanobacterial metabolite discovery.
abstract: Filamentous marine cyanobacteria produce bioactive natural products with both potential therapeutic value and capacity to be harmful to human health. Genome sequencing has revealed that cyanobacteria have the capacity to produce many more secondary metabolites than have been characterized. The biosynthetic pathways that encode cyanobacterial natural products are mostly uncharacterized, and lack of cyanobacterial genetic tools has largely prevented their heterologous expression. Hence, a combination of cutting edge and traditional techniques has been required to elucidate their secondary metabolite biosynthetic pathways. Here, we review the discovery and refined biochemical understanding of the olefin synthase and fatty acid ACP reductase/aldehyde deformylating oxygenase pathways to hydrocarbons, and the curacin A, jamaicamide A, lyngbyabellin, columbamide, and a trans-acyltransferase macrolactone pathway encoding phormidolide. We integrate into this discussion the use of genomics, mass spectrometric networking, biochemical characterization, and isolation and structure elucidation techniques.
authors.affiliation: Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California, San Diego, 9500 Gilman Drive MC0212, La Jolla, CA, 92093, USA. namoss@ucsd.edu.
authors.forename: Nathan A
authors.initials: NA
authors.lastname: Moss
authors.affiliation: Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California, San Diego, 9500 Gilman Drive MC0212, La Jolla, CA, 92093, USA.
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Bertin
authors.affiliation: Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California, San Diego, 9500 Gilman Drive MC0212, La Jolla, CA, 92093, USA.|Chair of Food Chemistry and Molecular Sensory Science, Technische Universität München, Lise-Meitner-Straße 34, 85354, Freising, Germany.
authors.forename: Karin
authors.initials: K
authors.lastname: Kleigrewe
authors.affiliation: Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California, San Diego, 9500 Gilman Drive MC0212, La Jolla, CA, 92093, USA.
authors.forename: Tiago F
authors.initials: TF
authors.lastname: Leão
authors.affiliation: Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California, San Diego, 9500 Gilman Drive MC0212, La Jolla, CA, 92093, USA.
authors.forename: Lena
authors.initials: L
authors.lastname: Gerwick
authors.affiliation: Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California, San Diego, 9500 Gilman Drive MC0212, La Jolla, CA, 92093, USA. wgerwick@ucsd.edu.|Skaggs School of Pharmacy, University of California, San Diego, 9500 Gilman Drive MC0212, La Jolla, CA, 92093, USA. wgerwick@ucsd.edu.
authors.forename: William H
authors.initials: WH
authors.lastname: Gerwick
mesh_terms: D001688:Biological Products / Q000737:chemistry / Q000378:metabolism; D053898:Biosynthetic Pathways / Q000235:genetics; D000458:Cyanobacteria / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism*; D023281:Genomics; D006801:Humans; D013058:Mass Spectrometry; D064210:Secondary Metabolism / Q000235:genetics
chemical_list: D001688:Biological Products
keywords: Biosynthesis; Cyanobacteria; Genomics; Mass spectrometry; Natural products