title: Microsatellite markers for Cryptolestes ferrugineus (Coleoptera: Laemophloeidae) and other Cryptolestes species.
abstract: Cryptolestes ferrugineus (Stephens, 1831) is an important insect pest of stored products. Due to its broad host range, short life cycle, and high reproductive capacity, this species has rapidly colonized temperate and tropical regions around the world. In this study, we isolated 18 novel polymorphic microsatellite loci from an enriched genomic library based on a biotin/streptavidin capture protocol. These loci will be useful tool to better understand the genetic structure and migration patterns of C. ferrugineus throughout the world. The genetic parameters were estimated based on 80 individual C. ferrugineus from two natural populations. The results revealed that 18 loci were different polymorphic levels. The numbers of alleles ranged from 3 to 12, and eleven loci demonstrated polymorphic information contents greater than 0.5. The observed (H O) and expected (H E) heterozygosities ranged from 0.051 to 0.883 and 0.173 to 0.815, respectively. Five locus/population combinations significantly deviated from Hardy-Weinberg equilibrium. We also demonstrated the potential utility of the C. ferrugineus microsatellites as population and species markers for four additional Cryptolestes species.
authors.affiliation: Academy of State Administration of Grain,No. 11 Baiwanzhuang Street,Beijing,China.
authors.forename: Y
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Academy of State Administration of Grain,No. 11 Baiwanzhuang Street,Beijing,China.
authors.forename: F
authors.initials: F
authors.lastname: Li
authors.affiliation: Department of Entomology,China Agricultural University,No. 2 Yuanmingyuan West Road,Beijing,China.
authors.forename: Z
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Pest Control of Stored Products and Food Safety,Crop Research Institute,Drnovská 507,Prague,Czech Republic.
authors.forename: V
authors.initials: V
authors.lastname: Stejskal
authors.affiliation: Department of Pest Control of Stored Products and Food Safety,Crop Research Institute,Drnovská 507,Prague,Czech Republic.
authors.forename: Z
authors.initials: Z
authors.lastname: Kučerová
authors.affiliation: Department of Entomology and Plant Pathology,Oklahoma State University,127 Noble Research Center,Stillwater,Oklahoma,USA.
authors.forename: G
authors.initials: G
authors.lastname: Opit
authors.affiliation: Department of Pest Control of Stored Products and Food Safety,Crop Research Institute,Drnovská 507,Prague,Czech Republic.
authors.forename: R
authors.initials: R
authors.lastname: Aulicky
authors.affiliation: Academy of State Administration of Grain,No. 11 Baiwanzhuang Street,Beijing,China.
authors.forename: T
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Academy of State Administration of Grain,No. 11 Baiwanzhuang Street,Beijing,China.
authors.forename: P
authors.initials: P
authors.lastname: He
authors.affiliation: Academy of State Administration of Grain,No. 11 Baiwanzhuang Street,Beijing,China.
authors.forename: Y
authors.initials: Y
authors.lastname: Cao
mesh_terms: D000818:Animals; D001517:Coleoptera / Q000145:classification / Q000235:genetics* / Q000502:physiology; D002523:Edible Grain / Q000469:parasitology; D018895:Microsatellite Repeats; D021141:Nucleic Acid Amplification Techniques; D011110:Polymorphism, Genetic / Q000235:genetics
keywords: Cryptolestes ferrugineus; Cryptolestes microsatellites; population genetics; primers