title: Oligonucleotide-Based Mimetics of Hepatocyte Growth Factor.
abstract: Oligonucleotide-based hepatocyte growth factor (HGF) mimetics are described. A DNA aptamer to Met, a cognate receptor for HGF, was shown to induce Met activation when used in dimer form. The most potent aptamer dimer, ss-0, which was composed solely of 100-mer single-stranded DNA, exhibited nanomolar potency. Aptamer ss-0 reproduced HGF-induced cellular behaviors, including migration and proliferation. The present work sheds light on oligonucleotides as a novel chemical entity for the design of growth factor mimetics.
authors.affiliation: Department of Chemistry and Biotechnology, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656 (Japan).
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Ueki
authors.affiliation: Department of Chemistry and Biotechnology, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656 (Japan).
authors.forename: Ayaka
authors.initials: A
authors.lastname: Ueki
authors.affiliation: Department of Chemistry and Biotechnology, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656 (Japan).
authors.forename: Naoto
authors.initials: N
authors.lastname: Kanda
authors.affiliation: Department of Chemistry and Biotechnology, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656 (Japan). ssando@chembio.t.u-tokyo.ac.jp.
authors.forename: Shinsuke
authors.initials: S
authors.lastname: Sando
mesh_terms: D001483:Base Sequence; D002460:Cell Line; D019281:Dimerization; D017228:Hepatocyte Growth Factor / Q000737:chemistry*; D006801:Humans; D018716:Molecular Mimicry; D009841:Oligonucleotides / Q000737:chemistry* / Q000235:genetics; D010766:Phosphorylation; D019859:Proto-Oncogene Proteins c-met / Q000235:genetics
chemical_list: D009841:Oligonucleotides; D017228:Hepatocyte Growth Factor; D019859:Proto-Oncogene Proteins c-met
keywords: DNA nanotechnology; aptamers; growth factors; receptors; signal transduction