title: MicroRNA-125b-5p attenuates lipopolysaccharide-induced monocyte chemoattractant protein-1 production by targeting inhibiting LACTB in THP-1 macrophages.
abstract: BACKGROUND
  Increasing evidence has shown that gene beta-lactamases (LACTB) has effect on obesity. Recent studies demonstrate that miR-125b-5p is a potential small molecular target to prevent atherosclerosis obliterans which may be inflammation-associated. However, the mechanism underlying miR-125b-5p on arteriosclerosis development, the association between miR-125b-5p and LACTB is still unknown.
  
  
  RESULTS
  In this study, we found that miR-125b-5p was down-regulated while LACTB was up-regulated in atherosclerotic plaques. Our results showed that LACTB was a potential target of miR-125b-5p based on bioinformatics analyses and dual-luciferase reporter assays. Moreover, miR-125b-5p directly inhibited LACTB protein and mRNA expression by targeting LACTB 3'UTR. Meanwhile, the expression of monocyte chemotactic protein-1 (MCP-1) was decreased by miR-125b-5p mimics treatment in THP-1 macrophages. We also demonstrated that the level of MCP-1 was markedly increased when transfected with LACTB. In addition, the upregulation of MCP-1 expression through miR-125b-5p inhibitors was attenuate by siRNA-LACTB treatment in LPS-stimulated THP-1 macrophages.
  
  
  CONCLUSIONS
  MiR-125b-5p attenuates the secretion of MCP-1 by directly targeting inhibiting LACTB in LPS-stimulated THP-1 macrophages.
authors.affiliation: Department of Vascular Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
authors.forename: Jing-Bo
authors.initials: JB
authors.lastname: Lu
authors.affiliation: Department of Vascular Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
authors.forename: Xing-Xing
authors.initials: XX
authors.lastname: Yao
authors.affiliation: Department of Cardiology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
authors.forename: Jian-Cheng
authors.initials: JC
authors.lastname: Xiu
authors.affiliation: Laboratory Medicine Center, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, China. Electronic address: ywhu0618@163.com.
authors.forename: Yan-Wei
authors.initials: YW
authors.lastname: Hu
mesh_terms: D000328:Adult; D050197:Atherosclerosis / Q000378:metabolism*; D002460:Cell Line; D018932:Chemokine CCL2 / Q000378:metabolism*; D005260:Female; D006801:Humans; D008070:Lipopolysaccharides; D008262:Macrophage Activation / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008565:Membrane Proteins / Q000037:antagonists & inhibitors* / Q000378:metabolism* / Q000494:pharmacology; D035683:MicroRNAs / Q000378:metabolism*; D008875:Middle Aged; D024101:Mitochondrial Proteins / Q000037:antagonists & inhibitors* / Q000378:metabolism* / Q000494:pharmacology; D001618:beta-Lactamases / Q000378:metabolism* / Q000494:pharmacology
chemical_list: C111835:CCL2 protein, human; D018932:Chemokine CCL2; D008070:Lipopolysaccharides; C531888:MIRN125 microRNA, human; D008565:Membrane Proteins; D035683:MicroRNAs; D024101:Mitochondrial Proteins; C486706:LACTB protein, human; D001618:beta-Lactamases
keywords: Inflammation; LACTB; MCP-1; THP-1 macrophages; miR-125b-5p