title: Application of 5-Fluorouracil-Polycaprolactone Sustained-Release Film in Ahmed Glaucoma Valve Implantation Inhibits Postoperative Bleb Scarring in Rabbit Eyes.
abstract: This study was designed to investigate whether 5-fluorouracil (5-Fu)-polycaprolactone sustained-release film in Ahmed glaucoma valve implantation inhibits postoperative bleb scarring in rabbit eyes. Eighteen New Zealand white rabbits were randomly divided into three groups (A, B and C; n = 6 per group). Group A received combined 5-Fu-polycaprolactone sustained-release film application and Ahmed glaucoma valve implantation, group B received local infiltration of 5-Fu and Ahmed glaucoma valve implantation, and group C received Ahmed glaucoma valve implantation. Postoperative observations were made of the anterior segment, intraocular pressure, central anterior chamber depth, blebs, drainage tube, and accompanying ciliary body detachment. The pathology of the blebs and surrounding tissues were observed at month 3 postoperatively. We revealed that the 5-Fu-polycaprolactone sustained-release film maintained a release concentration range of 13.7 ± 0.12 to 37.41 ± 0.47 μg/ml over three months in vitro. Postoperatively, diffuse blebs with ridges were found in all eyes in group A, two blebs were observed in group B, and no bleb formation was present in group C. The postoperative central anterior chamber depth in group A was significantly less than that of the other two groups. The postoperative intraocular pressure of group A stabilized at 6.33-8.67 mmHg, whereas that of group C gradually remained at 7.55-10.02 mmHg. The histopathology showed that the fibrous tissue thickness of the blebs in group A was significantly thinner than that of the other groups. We conclude that the 5-Fu-polycaprolactone sustained-release film had a sustained drug release effect, which promoted the inhibition of bleb scarring after Ahmed glaucoma valve implantation.
authors.affiliation: Hospital of Optometry and Ophthalmology, Wenzhou Medical College, Wenzhou, 325027, Zhejiang Province, China.
authors.forename: Xiu-Zeng
authors.initials: XZ
authors.lastname: Bi
authors.affiliation: Hospital of Optometry and Ophthalmology, Wenzhou Medical College, Wenzhou, 325027, Zhejiang Province, China.
authors.forename: Wei-Hua
authors.initials: WH
authors.lastname: Pan
authors.affiliation: Hospital of Optometry and Ophthalmology, Wenzhou Medical College, Wenzhou, 325027, Zhejiang Province, China.
authors.forename: Xin-Ping
authors.initials: XP
authors.lastname: Yu
authors.affiliation: Hospital of Optometry and Ophthalmology, Wenzhou Medical College, Wenzhou, 325027, Zhejiang Province, China.
authors.forename: Zong-Ming
authors.initials: ZM
authors.lastname: Song
authors.affiliation: School of Optometry and Ophthalmology, Wenzhou Medical College, Wenzhou, 325027, Zhejiang Province, China.
authors.forename: Zeng-Jin
authors.initials: ZJ
authors.lastname: Ren
authors.affiliation: School of Optometry and Ophthalmology, Wenzhou Medical College, Wenzhou, 325027, Zhejiang Province, China.
authors.forename: Min
authors.initials: M
authors.lastname: Sun
authors.affiliation: School of Optometry and Ophthalmology, Wenzhou Medical College, Wenzhou, 325027, Zhejiang Province, China.
authors.forename: Cong-Hui
authors.initials: CH
authors.lastname: Li
authors.affiliation: Wenzhou Engineering Research Center for Biomaterials, Wenzhou, 325027, Zhejiang Province, China.
authors.forename: Kai-Hui
authors.initials: KH
authors.lastname: Nan
mesh_terms: D000818:Animals; D000869:Anterior Eye Segment / Q000473:pathology / Q000601:surgery; D002921:Cicatrix / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*; D003692:Delayed-Action Preparations / Q000737:chemistry / Q000494:pharmacology*; D005472:Fluorouracil / Q000737:chemistry / Q000494:pharmacology*; D005901:Glaucoma / Q000473:pathology / Q000601:surgery / Q000628:therapy*; D020327:Glaucoma Drainage Implants; D007429:Intraocular Pressure / Q000187:drug effects; D011091:Polyesters / Q000737:chemistry; D011183:Postoperative Complications / Q000517:prevention & control*; D011817:Rabbits; D014065:Tonometry, Ocular; D016896:Treatment Outcome
chemical_list: D003692:Delayed-Action Preparations; D011091:Polyesters; C016240:polycaprolactone; D005472:Fluorouracil