title: Impact of Mitochondria-Mediated Apoptosis in U251 Cell Cycle Arrest in G1 Stage and Caspase Activation.
abstract: BACKGROUND Most mitochondria-mediated apoptosis has some relevance to the cell cycle, but there is still a lack of investigations about U251 cell cycle in human brain glioma cells. In this study, we aimed to clarify the correlation of mitochondria-mediated apoptosis with the U251 cell cycle and its influence on apoptosis, through observing the impact of mitochondria-mediated apoptosis in U251cell specificity cycle arrest and Caspase activation. MATERIAL AND METHODS AnnexinV/PI and API were used to label the brain glioma cells for flow cytometry analysis of U251 cell apoptosis and cell cycle. RT-PCR and Western blot were performed to detect Caspase-3 and Caspase-9 activation. RESULTS Peripheral blood in stationary phase is not sensitive to apoptosis induction, but U251 cells have obvious apoptosis. Mitochondria-mediated apoptosis mainly occurs in the G1 phase of the cell cycle. Caspase-3 and Caspase-9 mRNAs and proteins expression increased significantly after the cells were treated by mitochondrial apoptosis-related gene Bax induction. CONCLUSIONS Mitochondria-mediated apoptosis is related to the U251 cell cycle with specific G1 stage arrest. Caspase activation occurs in the process of cell apoptosis.
authors.affiliation: Department of Neurosurgery, Harbin Medical University Cancer Hospital, Harbin, Heilongjiang, China (mainland).
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Neurosurgery, Harbin Medical University Cancer Hospital, Harbin, Heilongjiang, China (mainland).
authors.forename: Peng
authors.initials: P
authors.lastname: Liang
authors.affiliation: Department of Neurosurgery, Harbin Medical University Cancer Hospital, Harbin, China (mainland).
authors.forename: Rui
authors.initials: R
authors.lastname: Zhang
mesh_terms: D017209:Apoptosis / Q000502:physiology*; D001932:Brain Neoplasms / Q000201:enzymology / Q000473:pathology*; D061945:Caspase Inhibitors / Q000378:metabolism*; D020169:Caspases / Q000378:metabolism*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000502:physiology; D059585:G1 Phase Cell Cycle Checkpoints / Q000502:physiology*; D005910:Glioma / Q000201:enzymology / Q000473:pathology*; D006801:Humans; D008928:Mitochondria / Q000201:enzymology / Q000502:physiology*; D015398:Signal Transduction
chemical_list: D061945:Caspase Inhibitors; D020169:Caspases