title: A label-free multi-functionalized graphene oxide based electrochemiluminscence immunosensor for ultrasensitive and rapid detection of Vibrio parahaemolyticus in seawater and seafood.
abstract: A label-free electrochemiluminescence (ECL) immunosensor for ultrasensitive and rapid detection of marine pathogenic bacterium Vibrio parahaemolyticus (VP) in seawater and seafood was developed based on multi-functionalized graphene oxide, which was prepared with N-(4-aminobutyl)-N-ethylisoluminol (ABEI) and VP antibody (anti-VP) simultaneously immobilized on the surface of magnetic graphene oxide (nanoFe3O4@GO). ABEI and anti-VP acted as the electrochemiluminophore and the capture device for VP respectively. Good conductivity and two-dimensional structure of the nanoFe3O4@GO enabled all the ABEI, immobilized on GO, electrochemically active and thus improved the detection sensitivity. Under optimal conditions, the ECL intensity decreased with increasing logarithmic concentrations of VP in the range of 10-10(8)CFU/mL, with a detection limit of 5CFU/mL for seawater and 5CFU/g for seafood. This ECL immunosensor showed high specificity, stability and reproducibility for the detection of VP. In addition, the ECL immunosensor has been successfully used to determine the concentration of VP in seawater and seafood rapidly, with a recovery of 94.4-112.0% and RSD 4.1-11.7%. Therefore, the developed immunosensor shows great prospect for practical application.
authors.affiliation: School of Materials Science and Chemical Engineering, Ningbo University, Ningbo 315211, PR China.
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Sha
authors.affiliation: School of Marine Sciences, Ningbo University, Ningbo 315211, PR China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Zhang
authors.affiliation: School of Materials Science and Chemical Engineering, Ningbo University, Ningbo 315211, PR China.
authors.forename: Wenrou
authors.initials: W
authors.lastname: Li
authors.affiliation: Faculty of Electrical Engineering and Computer Science, Ningbo University, Ningbo 315211, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wu
authors.affiliation: School of Materials Science and Chemical Engineering, Ningbo University, Ningbo 315211, PR China.
authors.forename: Sui
authors.initials: S
authors.lastname: Wang
authors.affiliation: School of Materials Science and Chemical Engineering, Ningbo University, Ningbo 315211, PR China. Electronic address: guozhiyong@nbu.edu.cn.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Guo
authors.affiliation: School of Marine Sciences, Ningbo University, Ningbo 315211, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhou
authors.affiliation: School of Marine Sciences, Ningbo University, Ningbo 315211, PR China. Electronic address: suxiurong@nbu.edu.cn.
authors.forename: Xiurong
authors.initials: X
authors.lastname: Su
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D002417:Cattle; D006108:Graphite / Q000737:chemistry*; D007118:Immunoassay / Q000379:methods*; D057230:Limit of Detection; D016014:Linear Models; D008163:Luminescent Measurements; D008958:Models, Molecular; D008968:Molecular Conformation; D010087:Oxides / Q000737:chemistry*; D015203:Reproducibility of Results; D017747:Seafood / Q000382:microbiology*; D012623:Seawater / Q000382:microbiology*; D013997:Time Factors; D014736:Vibrio parahaemolyticus / Q000302:isolation & purification*
chemical_list: D010087:Oxides; D006108:Graphite
keywords: Electrochemiluminescence immunosensor; Multi-functionalized graphene oxide; One-step; Vibrio parahaemolyticus