title: Assessment of canine autologous platelet-rich plasma produced with a commercial centrifugation and platelet recovery kit.
abstract: OBJECTIVE
  To characterize the cellular composition (platelets, erythrocytes, and leukocytes) and confirm reproducibility of platelet enrichment, as well as determine the platelet activation status in the final product of a commercial platelet-rich plasma kit using canine blood.
  
  
  METHODS
  Venous blood from 20 sedated client-owned dogs was used to prepare platelet-rich plasma (PRP) from a commercial kit. Complete blood counts were performed to determine erythrocyte, leukocyte, and platelet numbers in both whole blood (WB) and resultant PRP. The WB and PRP samples from jugular (fast collection) and cephalic (slow collection) venipuncture were also compared. P-selectin externalization was measured in WB and PRP samples from 15 of 20 dogs.
  
  
  RESULTS
  This commercial kit produced an average percent recovery in platelets of 64.7 ± 17.4; erythrocytes of 3.7 ± 0.8, and leukocytes of 31.6 ± 10.0. Neutrophil, monocyte, and lymphocyte percent recovery was 19.6 ± 7.2, 44.89 ± 19.8, and 57.5 ± 10.6, respectively. The recovery of platelets from jugular venipuncture (59.7 ± 13.6%) was lower than from cephalic recovery (68.8 ± 19.1%). The mean percent P-Selectin externalization for WB, PRP, and PRP with thrombin was 25.5 ± 30.9, 4.5 ± 6.4, and 90.6 ± 4.4 respectively.
  
  
  CONCLUSIONS
  Cellular reproducibility of this kit was confirmed and platelets were concentrated within autologous serum. Additionally, measurements of P-selectin externalization showed that platelets are inactive in PRP unless stimulated to degranulate.
authors.forename: Chris W
authors.initials: CW
authors.lastname: Frye
authors.forename: Andrew
authors.initials: A
authors.lastname: Enders
authors.forename: Marjory B
authors.initials: MB
authors.lastname: Brooks
authors.forename: Angela M
authors.initials: AM
authors.lastname: Struble
authors.affiliation: Joseph Wakshlag, Department of Clinical Sciences, Veterinary Medical Center, Cornell University, Ithaca, NY, United States, Phone: +1 607 253 4389, E-mail: jw37@cornell.edu.
authors.forename: Joseph J
authors.initials: JJ
authors.lastname: Wakshlag
mesh_terms: D000818:Animals; D001792:Blood Platelets; D001800:Blood Specimen Collection / Q000662:veterinary*; D002469:Cell Separation / Q000295:instrumentation* / Q000662:veterinary; D002498:Centrifugation; D004285:Dogs; D019007:P-Selectin / Q000032:analysis; D053657:Platelet-Rich Plasma / Q000166:cytology*
chemical_list: D019007:P-Selectin
keywords: P-selectin; PRP; Platelet-rich plasma; canine; leukocytes; platelet activation