title: Profiling of circulating microRNAs in patients with Barrett's esophagus and esophageal adenocarcinoma.
abstract: BACKGROUND
  Circulating microRNAs (miRNAs) have been suggested as novel markers for various diseases. The goal of this pilot study was to identify circulating miRNAs differentially expressed comparing Barrett's esophagus (BE), esophageal adenocarcinoma (EAC), and controls.
  
  
  METHODS
  MicroRNA expression profiling was performed by qPCR array using plasma from six controls and eight BE and eight EAC patients. Validation was performed by analyzing the expression of six selected miRNAs, by qRT-PCR in 115 plasma samples of controls, BE, and EAC patients. Diagnostic accuracy was evaluated by area under the curve (AUC) analysis.
  
  
  RESULTS
  We identified three miRNAs that were elevated in EAC and four miRNAs that were elevated in BE. Further validation showed that miRNA-382-5p was significantly increased and miRNA-133a-3p significantly decreased in EAC. miRNA-194-5p and miRNA-451a were significantly increased and miRNA-136-5p significantly decreased in BE versus controls. A combination of three or more miRNAs was found to have a good diagnostic performance in discriminating BE from controls (AUC: 0.832), EAC from controls (AUC: 0.846), and BE from EAC (AUC: 0.797).
  
  
  CONCLUSIONS
  Our data suggest that circulating miRNAs are differentially expressed in BE and EAC. The miRNAs identified may be used for future non-invasive screening of BE and EAC.
authors.affiliation: Department of Gastroenterology and Hepatology, University Medical Center Utrecht, Heidelberglaan 100, 3584 CX, Utrecht, The Netherlands.
authors.forename: Pauline
authors.initials: P
authors.lastname: Bus
authors.affiliation: Department of Gastroenterology and Hepatology, University Medical Center Utrecht, Heidelberglaan 100, 3584 CX, Utrecht, The Netherlands.
authors.forename: Christine
authors.initials: C
authors.lastname: Kestens
authors.affiliation: Department of Pathology, University Medical Center Utrecht, Heidelberglaan 100, 3584 CX, Utrecht, The Netherlands.
authors.forename: Fiebo Jan Willem
authors.initials: FJ
authors.lastname: Ten Kate
authors.affiliation: Department of Gastroenterology and Hepatology, Radboud University Medical Center, Geert Grooteplein 8, 6525 GA, Nijmegen, The Netherlands.
authors.forename: Wilbert
authors.initials: W
authors.lastname: Peters
authors.affiliation: Department of Gastroenterology and Hepatology, Radboud University Medical Center, Geert Grooteplein 8, 6525 GA, Nijmegen, The Netherlands.
authors.forename: Joost Paulus Hubertus
authors.initials: JP
authors.lastname: Drenth
authors.affiliation: Department of Medical Oncology, University Medical Center Utrecht, Heidelberglaan 100, 3584 CX, Utrecht, The Netherlands.
authors.forename: Jeanine Merel Leonoor
authors.initials: JM
authors.lastname: Roodhart
authors.affiliation: Department of Gastroenterology and Hepatology, University Medical Center Utrecht, Heidelberglaan 100, 3584 CX, Utrecht, The Netherlands.
authors.forename: Peter Derk
authors.initials: PD
authors.lastname: Siersema
authors.affiliation: Department of Gastroenterology and Hepatology, University Medical Center Utrecht, Heidelberglaan 100, 3584 CX, Utrecht, The Netherlands. j.w.p.m.vanbaal-2@umcutrecht.nl.
authors.forename: Jantine Wilhelmina Paula Maria
authors.initials: JW
authors.lastname: van Baal
mesh_terms: D000230:Adenocarcinoma / Q000097:blood* / Q000175:diagnosis; D000328:Adult; D000368:Aged; D001471:Barrett Esophagus / Q000097:blood* / Q000175:diagnosis; D016022:Case-Control Studies; D004938:Esophageal Neoplasms / Q000097:blood* / Q000175:diagnosis; D005260:Female; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged; D010865:Pilot Projects; D011237:Predictive Value of Tests; D012372:ROC Curve
chemical_list: D035683:MicroRNAs
keywords: Barrett’s esophagus; Circulating biomarker; Esophageal adenocarcinoma; MicroRNA; Plasma