title: Expression of ZFX gene correlated with the central features of the neoplastic phenotype in human brain tumors with distinct phenotypes.
abstract: BACKGROUND
  The zinc finger transcription factor zinc finger protein, X-linked (ZFX) acts as an important director of self-renewal in several stem cell types. Moreover, ZFX expression abnormally increases in various cancers and relates to tumor grade. We performed this study, to examine its role in the pathogenesis of astrocytoma and meningioma.
  
  
  METHODS
  We used real-time reverse transcription polymerase chain reaction method for evaluation of ZFX expression in 25 astrocytoma tumoral tissue and 25 meningioma tumoral tissues with different WHO grades. Furthermore, the association of gene expression with various clinic-pathological characteristics was examined.
  
  
  RESULTS
  We found that there is a significant association between gene expression and different tumor grades, the presence or absence of invasion, forming and nonforming of glomeruloid vessels, the age over or under 50 and the presence or absence of calcification in astrocytomas. This is the first report that shows that ZFX was directly correlated with the central features of the neoplastic phenotype, including the growth of cancer cells, angiogenesis, and invasion.
  
  
  CONCLUSIONS
  Regarding all the above-mentioned studies, it is highly plausible that silencing the expression of ZFX gene in gliomas has a major role in the therapeutic interventions of the disease in future.
authors.affiliation: Department of Genetics, Faculty of Basic Sciences, Shahrekord University, Shahrekord, Iran.
authors.forename: Azita
authors.initials: A
authors.lastname: Afzali
authors.affiliation: Department of Genetics, Faculty of Basic Sciences, Shahrekord University, Shahrekord, Iran.
authors.forename: Modjtaba
authors.initials: M
authors.lastname: Emadi-Baygi
authors.affiliation: Department of Genetics and Molecular Biology, School of Medicine, Pediatric Inherited Diseases Research Center, Research Institute for Primordial Prevention of Non-communicable Disease, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Parvaneh
authors.initials: P
authors.lastname: Nikpour
authors.affiliation: Department of Genetics and Molecular Biology, School of Medicine, Pediatric Inherited Diseases Research Center, Research Institute for Primordial Prevention of Non-communicable Disease, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Fatemehe
authors.initials: F
authors.lastname: Nazemroaya
authors.affiliation: Department of Genetics and Molecular Biology, School of Medicine, Pediatric Inherited Diseases Research Center, Research Institute for Primordial Prevention of Non-communicable Disease, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Majid
authors.initials: M
authors.lastname: Kheirollahi
keywords: Astrocytoma; X-linked; brain tumor; expression; meningioma; zinc finger protein