title: Genomic Analysis of Detoxification Supergene Families in the Mosquito Anopheles sinensis.
abstract: Anopheles sinensis is an important malaria vector in China and other Southeast Asian countries, and the emergence of insecticide resistance in this mosquito poses a serious threat to the efficacy of malaria control programs. The recently published An. sinensis genome and transcriptome provide an opportunity to understand the molecular mechanisms of insecticide resistance. Analysis of the An. sinensis genome revealed 174 detoxification genes, including 93 cytochrome P450s (P450s), 31 glutathione-S-transferases (GSTs), and 50 choline/carboxylesterases (CCEs). The gene number was similar to that in An. gambiae, but represented a decrease of 29% and 42% compared with Aedes aegypti and Culex quinquefasciatus, respectively. The considerable contraction in gene number in Anopheles mosquitoes mainly occurred in two detoxification supergene families, P450s and CCEs. The available An. sinensis transcriptome was also re-analyzed to further identify key resistance-associated detoxification genes. Among 174 detoxification genes, 124 (71%) were detected. Several candidate genes overexpressed in a deltamethrin-resistant strain (DR-strain) were identified as belonging to the CYP4 or CYP6 family of P450s and the Delta GST class. These generated data provide a basis for identifying the resistance-associated genes of An. sinensis at the molecular level.
authors.affiliation: Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu, 210029, P. R. China.
authors.forename: Dan
authors.initials: D
authors.lastname: Zhou
authors.affiliation: Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu, 210029, P. R. China.
authors.forename: Xianmiao
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu, 210029, P. R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu, 210029, P. R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Ma
authors.affiliation: Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu, 210029, P. R. China.
authors.forename: Bo
authors.initials: B
authors.lastname: Shen
authors.affiliation: Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu, 210029, P. R. China.
authors.forename: Changliang
authors.initials: C
authors.lastname: Zhu
mesh_terms: D000818:Animals; D000852:Anopheles / Q000145:classification / Q000235:genetics*; D043182:Carboxylesterase / Q000235:genetics / Q000378:metabolism; D003465:Culex / Q000235:genetics; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000378:metabolism; D016678:Genome; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008658:Inactivation, Metabolic / Q000235:genetics*; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D007303:Insect Vectors / Q000235:genetics*; D007305:Insecticide Resistance / Q000235:genetics*; D007306:Insecticides; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D008288:Malaria / Q000517:prevention & control / Q000635:transmission; D058977:Molecular Sequence Annotation; D009570:Nitriles; D010802:Phylogeny; D011722:Pyrethrins; D059467:Transcriptome
chemical_list: D019476:Insect Proteins; D007306:Insecticides; D007527:Isoenzymes; D009570:Nitriles; D011722:Pyrethrins; C017180:decamethrin; D003577:Cytochrome P-450 Enzyme System; D005982:Glutathione Transferase; D043182:Carboxylesterase