title: Determination of Phosphatidylcholine Hydroperoxide (PCOOH) as a Marker of Membrane Lipid Peroxidation.
abstract: Increasing evidence for phosphatidylcholine hydroperoxide (PCOOH) as a marker of oxidative food deterioration and oxidative diseases has revealed the need for a pure PCOOH standard and a reliable quantification method. Recently, we synthesized the PCOOH isomers 1-palmitoyl-2-(9-hydroperoxyoctadecadienoyl)-sn-glycero-3-phosphocholine, (16:0/9-HpODE PC) and 1-palmitoyl-2-(13-hydroperoxyoctadecadienoyl)-sn-glycero-3-phosphocholine (16:0/13-HpODE PC). Using these standards along with liquid chromatography-tandem mass spectrometry, a reliable quantification method was developed. This mini-review describes these analytical techniques, with a particular emphasis on clinical sample analysis.
authors.affiliation: Food and Biodynamic Chemistry Laboratory, Graduate School of Agricultural Science, Tohoku University.
authors.forename: Kiyotaka
authors.initials: K
authors.lastname: Nakagawa
authors.forename: Shunji
authors.initials: S
authors.lastname: Kato
authors.forename: Teruo
authors.initials: T
authors.lastname: Miyazawa
mesh_terms: D050197:Atherosclerosis / Q000097:blood / Q000175:diagnosis; D015415:Biomarkers / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007536:Isomerism; D008041:Linoleic Acids / Q000097:blood; D015227:Lipid Peroxidation; D008054:Lipid Peroxides / Q000097:blood; D018384:Oxidative Stress; D010713:Phosphatidylcholines / Q000097:blood*; D053719:Tandem Mass Spectrometry
chemical_list: D015415:Biomarkers; D008041:Linoleic Acids; D008054:Lipid Peroxides; D010713:Phosphatidylcholines; C057590:phosphatidylcholine hydroperoxide; C038649:13-hydroperoxy-9,11-octadecadienoic acid; C049606:9-hydroperoxy-11,12-octadecadienoic acid