title: The investigation of anti-inflammatory activity of volatile oil of Angelica sinensis by plasma metabolomics approach.
abstract: Angelica sinensis (AS) is an important medicinal plant, and volatile oil is the main pharmacologically active ingredient. This study was aimed to investigate the anti-inflammatory activity of the volatile oil of A. sinensis (VOAS) and explore its potential anti-inflammatory mechanism by plasma metabolomics approach. Rat acute inflammation was induced by subcutaneous injection of carrageenan in hind paws. Paw edema, histamine (HIS) and 5-hydroxytryptamine (5-HT) were detected. Then, we analyzed plasma metabolic profiling of acute inflammation and performed pathway analysis on the metabolite markers reversed after VOAS administration and further integration of metabolic networks. The results showed that VOAS could alleviate the paw edema and decrease plasma HIS and 5-HT levels. Fourteen metabolite markers of acute inflammation were screened, and the levels were all reversed to different degrees after VOAS administration. These metabolite markers mainly related to linoleic acid metabolism, ascorbate and aldarate metabolism, arachidonic acid metabolism, glyoxylate and dicarboxylate metabolism, and glycine, serine and threonine metabolism. In metabolic networks, glycine and arachidonic acid were node molecules. It indicated that VOAS could significantly inhibit systemic inflammatory response triggered by acute local stimulation and it exerted anti-inflammatory activity mainly through regulating the disturbed metabolic networks centered on glycine and arachidonic acid.
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, PR China.
authors.forename: Wanling
authors.initials: W
authors.lastname: Yao
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, PR China.
authors.forename: Ling
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, PR China.
authors.forename: Yongli
authors.initials: Y
authors.lastname: Hua
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, PR China.
authors.forename: Peng
authors.initials: P
authors.lastname: Ji
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, PR China.
authors.forename: Pengling
authors.initials: P
authors.lastname: Li
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, PR China.
authors.forename: Jinxia
authors.initials: J
authors.lastname: Li
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, PR China.
authors.forename: Lijia
authors.initials: L
authors.lastname: Zhong
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, PR China.
authors.forename: Haifu
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, PR China. Electronic address: weiym@gsau.edu.cn.
authors.forename: Yanming
authors.initials: Y
authors.lastname: Wei
mesh_terms: D029971:Angelica sinensis / Q000737:chemistry* / Q000633:toxicity; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D016718:Arachidonic Acid / Q000378:metabolism; D002351:Carrageenan; D004487:Edema / Q000139:chemically induced / Q000517:prevention & control; D005998:Glycine / Q000378:metabolism; D006636:Histamine Release / Q000187:drug effects; D007249:Inflammation / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control; D007928:Lethal Dose 50; D008297:Male; D053858:Metabolic Networks and Pathways / Q000187:drug effects; D055432:Metabolomics / Q000379:methods*; D009822:Oils, Volatile / Q000494:pharmacology* / Q000633:toxicity; D051381:Rats; D012701:Serotonin / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D009822:Oils, Volatile; D016718:Arachidonic Acid; D012701:Serotonin; D002351:Carrageenan; D005998:Glycine
keywords: Anti-inflammation; Metabolomics; Volatile oil of Angelica sinensis (VOAS)