title: Click and release: fluoride cleavable linker for mild bioorthogonal separation.
abstract: Herein, we present a water dispersable, magnetic nanoparticle supported "click and release" system. The cleavable linker has been synthesized by using a strain-promoted copper-free "click" reagent to establish the specific link and a fluoride cleavable silane moiety for mild cleavage. Small organic molecules, azide-bearing dyes and functionalized enzymes have been bound to the magnetic particle and released in a bioorthogonal way.
authors.affiliation: Institute for Chemical and Bioengineering, ETH Zurich, Vladimir-Prelog-Weg 1, 8093 Zurich, Switzerland. Wendelin.stark@chem.ethz.ch.
authors.forename: Elia M
authors.initials: EM
authors.lastname: Schneider
authors.affiliation: Institute for Chemical and Bioengineering, ETH Zurich, Vladimir-Prelog-Weg 1, 8093 Zurich, Switzerland. Wendelin.stark@chem.ethz.ch.
authors.forename: Martin
authors.initials: M
authors.lastname: Zeltner
authors.affiliation: Institute for Chemical and Bioengineering, ETH Zurich, Vladimir-Prelog-Weg 1, 8093 Zurich, Switzerland. Wendelin.stark@chem.ethz.ch.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Zlateski
authors.affiliation: Institute for Chemical and Bioengineering, ETH Zurich, Vladimir-Prelog-Weg 1, 8093 Zurich, Switzerland. Wendelin.stark@chem.ethz.ch.
authors.forename: Robert N
authors.initials: RN
authors.lastname: Grass
authors.affiliation: Institute for Chemical and Bioengineering, ETH Zurich, Vladimir-Prelog-Weg 1, 8093 Zurich, Switzerland. Wendelin.stark@chem.ethz.ch.
authors.forename: Wendelin J
authors.initials: WJ
authors.lastname: Stark
mesh_terms: D000818:Animals; D001386:Azides / Q000737:chemistry / Q000302:isolation & purification*; D002417:Cattle; D002918:Chymotrypsin / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D057930:Click Chemistry; D005456:Fluorescent Dyes / Q000737:chemistry / Q000302:isolation & purification*; D005459:Fluorides / Q000737:chemistry*; D058185:Magnetite Nanoparticles / Q000737:chemistry; D012821:Silanes / Q000737:chemistry; D014867:Water / Q000737:chemistry
chemical_list: D001386:Azides; D005456:Fluorescent Dyes; D058185:Magnetite Nanoparticles; D012821:Silanes; D014867:Water; D002918:Chymotrypsin; C117608:alpha-chymotrypsin; D005459:Fluorides