title: Repair of peripheral nerve defects with chemically extracted acellular nerve allografts loaded with neurotrophic factors-transfected bone marrow mesenchymal stem cells.
abstract: Chemically extracted acellular nerve allografts loaded with brain-derived neurotrophic factor-transfected or ciliary neurotrophic factor-transfected bone marrow mesenchymal stem cells have been shown to repair sciatic nerve injury better than chemically extracted acellular nerve allografts alone, or chemically extracted acellular nerve allografts loaded with bone marrow mesenchymal stem cells. We hypothesized that these allografts compounded with both brain-derived neurotrophic factor- and ciliary neurotrophic factor-transfected bone marrow mesenchymal stem cells may demonstrate even better effects in the repair of peripheral nerve injury. We cultured bone marrow mesenchymal stem cells expressing brain-derived neurotrophic factor and/or ciliary neurotrophic factor and used them to treat sciatic nerve injury in rats. We observed an increase in sciatic functional index, triceps wet weight recovery rate, myelin thickness, number of myelinated nerve fibers, amplitude of motor-evoked potentials and nerve conduction velocity, and a shortened latency of motor-evoked potentials when allografts loaded with both neurotrophic factors were used, compared with allografts loaded with just one factor. Thus, the combination of both brain-derived neurotrophic factor and ciliary neurotrophic factor-transfected bone marrow mesenchymal stem cells can greatly improve nerve injury.
authors.affiliation: Medical College, Henan Polytechnic University, Jiaozuo, Henan Province, China ; Institute of Clinical Anatomy, Southern Medical University, Guangzhou, Guangdong Province, China.
authors.forename: Yan-Ru
authors.initials: YR
authors.lastname: Zhang
authors.affiliation: Medical College, Henan Polytechnic University, Jiaozuo, Henan Province, China.
authors.forename: Ka
authors.initials: K
authors.lastname: Ka
authors.affiliation: Department of Orthopedics, First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan Province, China.
authors.forename: Ge-Chen
authors.initials: GC
authors.lastname: Zhang
authors.affiliation: Department of Orthopedics, First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan Province, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Laboratory Medicine, Children's Hospital of Zhengzhou, Zhengzhou, Henan Province, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Shang
authors.affiliation: School of Basic Medical Sciences, Zhengzhou University, Zhengzhou, Henan Province, China.
authors.forename: Guo-Qiang
authors.initials: GQ
authors.lastname: Zhao
authors.affiliation: Institute of Clinical Anatomy, Southern Medical University, Guangzhou, Guangdong Province, China.
authors.forename: Wen-Hua
authors.identifier: 0000-0001-6965-5898
authors.initials: WH
authors.lastname: Huang
keywords: bone marrow mesenchymal stem cells; brain-derived neurotrophic factor; chemically extracted acellular nerve allografts; ciliary neurotrophic factor; nerve regeneration; neural regeneration; peripheral nerve; peripheral nerve injury