title: Characteristic Changes of Astrocyte and Microglia in Rat Striatum Induced by 3-NP and MCAO.
abstract: Our previous studies had confirmed that both 3-NP and MCAO induced the behavioral defect as well as striatal neuronal injury and loss in experimental rats. This study aimed to examine different response forms of striatal astrocyte and microglia in 3-NP and MCAO rat models. The present results showed that the immunoreaction for GFAP was extremely weak in the lesioned core of striatum, but in the transition zone of 3-NP model and the penumbra zone of MCAO model, GFAP+ cells showed strong hypertrophic and proliferative changes. Statistical analysis for the number, size and integral optical density (IOD) of GFAP+ cells showed significant differences when compared with their controls and compared between the core and the transition zone or the penumbra zone, respectively, but no differences between the 3-NP and MCAO groups. However, Iba-1+ cells showed obvious hypertrophy and proliferation in the injured striatum in the 3-NP and the MCAO models, especially in the transition zone of 3-NP model and the penumbra zone of MCAO model. These Iba-1+ cells displayed two characteristic forms as branching cells with thick processes and amoeboid cells with thin processes. Statistical analysis showed that the number, size and IOD of Iba-1+ cells were significantly increased in the cores and the transition zone of 3-NP group and the penumbra zone of MCAO group than that of the controls, and the immune response of Iba-1 was stronger in the MCAO group than in the 3-NP group. The present results suggested that characteristic responses of astrocyte and microglia in the 3-NP and the MCAO models display their different effects on the pathological process of brain injury.
authors.affiliation: Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen, China.
authors.forename: Shuhua
authors.initials: S
authors.lastname: Mu
authors.affiliation: Department of Anesthesiology, Guangdong No. 2 Provincial People's Hospital, Guangdong Provincial Emergency Hospital, Guangzhou, China.
authors.forename: Bingbing
authors.initials: B
authors.lastname: Liu
authors.affiliation: Department of Anatomy, Zhongshan School of Medicine, SUN Yat-sen University, 74 Zhongshan Rd 2, Guangzhou, 510080, China.
authors.forename: Lisi
authors.initials: L
authors.lastname: Ouyang
authors.affiliation: Department of Anatomy, Zhongshan School of Medicine, SUN Yat-sen University, 74 Zhongshan Rd 2, Guangzhou, 510080, China.
authors.forename: Mali
authors.initials: M
authors.lastname: Zhan
authors.affiliation: Department of Anatomy, Zhongshan School of Medicine, SUN Yat-sen University, 74 Zhongshan Rd 2, Guangzhou, 510080, China.
authors.forename: Si
authors.initials: S
authors.lastname: Chen
authors.affiliation: Department of Anatomy, Zhongshan School of Medicine, SUN Yat-sen University, 74 Zhongshan Rd 2, Guangzhou, 510080, China.
authors.forename: Jiajia
authors.initials: J
authors.lastname: Wu
authors.affiliation: Department of Anatomy, Zhongshan School of Medicine, SUN Yat-sen University, 74 Zhongshan Rd 2, Guangzhou, 510080, China.
authors.forename: Jiachang
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Anatomy, Zhongshan School of Medicine, SUN Yat-sen University, 74 Zhongshan Rd 2, Guangzhou, 510080, China.
authors.forename: Xianyou
authors.initials: X
authors.lastname: Wei
authors.affiliation: Department of Anatomy, Zhongshan School of Medicine, SUN Yat-sen University, 74 Zhongshan Rd 2, Guangzhou, 510080, China.
authors.forename: Weiping
authors.initials: W
authors.lastname: Wang
authors.affiliation: Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen, China. jzhanghappy@163.com.|School of Medicine, Shenzhen University, Nanhai Ave 3688, Shenzhen, 518060, China. jzhanghappy@163.com.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Anatomy, Zhongshan School of Medicine, SUN Yat-sen University, 74 Zhongshan Rd 2, Guangzhou, 510080, China. leiwl@mail.sysu.edu.cn.
authors.forename: Wanlong
authors.initials: W
authors.lastname: Lei
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000187:drug effects / Q000473:pathology*; D002135:Calcium-Binding Proteins / Q000378:metabolism; D002452:Cell Count; D049109:Cell Proliferation; D048429:Cell Size; D003342:Corpus Striatum / Q000187:drug effects / Q000473:pathology*; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D020244:Infarction, Middle Cerebral Artery / Q000473:pathology*; D008297:Male; D008840:Microfilament Proteins / Q000378:metabolism; D017628:Microglia / Q000187:drug effects / Q000473:pathology*; D009574:Nitro Compounds / Q000494:pharmacology*; D011422:Propionates / Q000494:pharmacology*; D017207:Rats, Sprague-Dawley
chemical_list: C100745:Aif1 protein, rat; D002135:Calcium-Binding Proteins; D005904:Glial Fibrillary Acidic Protein; D008840:Microfilament Proteins; D009574:Nitro Compounds; D011422:Propionates; C015392:3-nitropropionic acid
keywords: 3-NP; Astrocyte; MCAO; Microglia; Striatum