title: Distinct Profiles of Soluble Cytokine Receptors Between B-Cell Vitreoretinal Lymphoma and Uveitis.
abstract: OBJECTIVE
  To determine the profiles of soluble cytokine receptors and cytokines, including mostly their ligands, in the vitreous humor of patients with B-cell vitreoretinal lymphoma (VRL) and uveitis.
  
  
  METHODS
  Vitreous samples were collected from immunocompetent patients with VRL (n = 21), uveitis (n = 20), and idiopathic epiretinal membrane (n = 21) as controls. Cytometric beads assay were used to determine the vitreous concentrations of soluble receptors and cytokines.
  
  
  RESULTS
  Vitreous levels of soluble IL-2 receptor α (sIL-2Rα), sIL-6R, soluble tumor necrosis factor receptor (TNFR) 1, sTNFR2, soluble vascular endothelial growth factor receptor (sVEGFR) 1, sVEGFR2, and IL-10 were higher in patients with VRL than in those with uveitis and controls, whereas those of sIL-1R1, sIL-1R2, and sIL-4R were higher in patients with uveitis than those with VRL and controls. In analyses in patients with VRL, elevation of sVEGFR1 and sVEGFR2 levels was more prominent in patients with systemic metastatic retinal lymphoma (SMRL) than in those with primary VRL/primary central nervous system lymphoma (PVRL/PCNSL). Furthermore, sIL-2Rα levels were increased in patients with VRL who developed subretinal lesions compared with in those who mainly had vitreous cavity opacity, positively correlated with the density of CD3+ cells in the vitrectomy cell blocks.
  
  
  CONCLUSIONS
  The profiles of soluble cytokine receptors and cytokines in patients with VRL were different from those with uveitis. In addition, sVEGFR1 and sVEGFR2 levels may be differential diagnostic markers between PVRL/PCNSL and SMRL, and sIL-2Rα levels can anticipate infiltration of VRL cells into the subretina and/or retina.
authors.affiliation: Department of Ophthalmology Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
authors.forename: Atsunobu
authors.initials: A
authors.lastname: Takeda
authors.affiliation: Department of Ophthalmology Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yoshikawa
authors.affiliation: Department of Ophthalmology Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
authors.forename: Takako
authors.initials: T
authors.lastname: Fukuhara
authors.affiliation: Department of Ophthalmology Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
authors.forename: Shin-Ichi
authors.initials: S
authors.lastname: Hikita
authors.affiliation: Department of Ophthalmology Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
authors.forename: Kuniaki
authors.initials: K
authors.lastname: Hijioka
authors.affiliation: Department of Ophthalmology Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
authors.forename: Takaaki
authors.initials: T
authors.lastname: Otomo
authors.affiliation: Department of Ophthalmology Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
authors.forename: Ryoichi
authors.initials: R
authors.lastname: Arita
authors.affiliation: Department of Ophthalmology Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
authors.forename: Toshio
authors.initials: T
authors.lastname: Hisatomi
authors.affiliation: Department of Ophthalmology, Graduate School of Medicine, Yamaguchi University, Ube, Yamaguchi, Japan.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Kimura
authors.affiliation: Department of Ophthalmology Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
authors.forename: Shigeo
authors.initials: S
authors.lastname: Yoshida
authors.affiliation: Department of Ophthalmology, Faculty of Medicine, Fukuoka Dental College, Fukuoka, Japan.
authors.forename: Yo-Ichi
authors.initials: Y
authors.lastname: Kawano
authors.affiliation: Department of Ophthalmology Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan 2Department of Ophthalmology, Graduate School of Medicine, Yamaguchi University, Ube, Yamaguchi, Japan.
authors.forename: Koh-Hei
authors.initials: KH
authors.lastname: Sonoda
authors.affiliation: Department of Ophthalmology Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
authors.forename: Tatsuro
authors.initials: T
authors.lastname: Ishibashi
mesh_terms: D000368:Aged; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D016393:Lymphoma, B-Cell / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D008297:Male; D008875:Middle Aged; D018121:Receptors, Cytokine / Q000378:metabolism*; D019572:Retinal Neoplasms / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D014605:Uveitis / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D014821:Vitrectomy; D014822:Vitreous Body / Q000378:metabolism* / Q000473:pathology
chemical_list: D018121:Receptors, Cytokine