title: Chromatin Immunoprecipitation-Sequencing (ChIP-seq) for Mapping of Estrogen Receptor-Chromatin Interactions in Breast Cancer.
abstract: Chromatin immunoprecipitation-sequencing (ChIP-Seq) is a powerful tool which combines the established method of ChIP with next-generation sequencing (NGS) to determine DNA-binding sites of a protein of interest on a genome-wide level, importantly, allowing for de novo discovery of binding events. Here we describe ChIP-seq using the well-established example of estrogen receptor-α mapping in the MCF7 breast cancer cell line.
authors.affiliation: Cambridge Research Institute, Cancer Research UK, University of Cambridge, Robinson Way, Cambridge, CB2 0RE, UK.
authors.forename: Kelly A
authors.initials: KA
authors.lastname: Holmes
authors.affiliation: Cambridge Research Institute, Cancer Research UK, University of Cambridge, Robinson Way, Cambridge, CB2 0RE, UK.
authors.forename: Gordon D
authors.initials: GD
authors.lastname: Brown
authors.affiliation: Cambridge Research Institute, Cancer Research UK, University of Cambridge, Robinson Way, Cambridge, CB2 0RE, UK. jason.carroll@cruk.cam.ac.uk.
authors.forename: Jason S
authors.initials: JS
authors.lastname: Carroll
mesh_terms: D001665:Binding Sites; D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism*; D002843:Chromatin / Q000235:genetics / Q000378:metabolism*; D047369:Chromatin Immunoprecipitation; D019295:Computational Biology; D030541:Databases, Genetic; D047628:Estrogen Receptor alpha / Q000235:genetics / Q000378:metabolism*; D005260:Female; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D061986:MCF-7 Cells; D016133:Polymerase Chain Reaction; D011485:Protein Binding; D017422:Sequence Analysis, DNA; D057188:Workflow
chemical_list: D002843:Chromatin; C506487:ESR1 protein, human; D047628:Estrogen Receptor alpha
keywords: Chromatin; Chromatin immunoprecipitation; Enhancers; Estrogen receptor; Next-generation sequencing; Promoters