title: BODIPY-Coumarin Conjugate as an Endoplasmic Reticulum Membrane Fluidity Sensor and Its Application to ER Stress Models.
abstract: An endoplasmic reticulum (ER) membrane-selective chemosensor composed of BODIPY and coumarin moieties and a long alkyl chain (n-C18) was synthesized. The emission ratio of BODIPY to coumarin depends on the solution viscosity. The probe is localized to the ER membrane and was applied to reveal the reduced ER membrane fluidity under ER stress conditions.
authors.affiliation: The School of East-West Medical Science, Kyung Hee University , Yongin 446-701, Korea.
authors.forename: Hoyeon
authors.initials: H
authors.lastname: Lee
authors.affiliation: Department of Chemistry, Korea University , Seoul 136-701, Korea.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Yang
authors.affiliation: The School of East-West Medical Science, Kyung Hee University , Yongin 446-701, Korea.
authors.forename: Youngjin
authors.initials: Y
authors.lastname: Wi
authors.affiliation: The School of East-West Medical Science, Kyung Hee University , Yongin 446-701, Korea.
authors.forename: Tae Woo
authors.initials: TW
authors.lastname: Kim
authors.affiliation: Department of Chemistry, Korea University , Seoul 136-701, Korea.
authors.forename: Peter
authors.initials: P
authors.lastname: Verwilst
authors.affiliation: Department of Chemistry, Korea University , Seoul 136-701, Korea.
authors.forename: Yun Hak
authors.initials: YH
authors.lastname: Lee
authors.affiliation: The School of East-West Medical Science, Kyung Hee University , Yongin 446-701, Korea.
authors.forename: Ga-In
authors.initials: GI
authors.lastname: Han
authors.affiliation: The School of East-West Medical Science, Kyung Hee University , Yongin 446-701, Korea.
authors.forename: Chulhun
authors.initials: C
authors.lastname: Kang
authors.affiliation: Department of Chemistry, Korea University , Seoul 136-701, Korea.
authors.forename: Jong Seung
authors.initials: JS
authors.lastname: Kim
mesh_terms: D001896:Boron Compounds / Q000737:chemistry*; D003374:Coumarins / Q000737:chemistry*; D004721:Endoplasmic Reticulum / Q000737:chemistry* / Q000378:metabolism; D059865:Endoplasmic Reticulum Stress; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D008560:Membrane Fluidity; D008856:Microscopy, Fluorescence; D013050:Spectrometry, Fluorescence
chemical_list: C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D003374:Coumarins; D005456:Fluorescent Dyes; C030123:coumarin