title: NeuroGPS-Tree: automatic reconstruction of large-scale neuronal populations with dense neurites.
abstract: The reconstruction of neuronal populations, a key step in understanding neural circuits, remains a challenge in the presence of densely packed neurites. Here we achieved automatic reconstruction of neuronal populations by partially mimicking human strategies to separate individual neurons. For populations not resolvable by other methods, we obtained recall and precision rates of approximately 80%. We also demonstrate the reconstruction of 960 neurons within 3 h.
authors.affiliation: Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan, China.|Ministy of Education Key Laboratory for Biomedical Photonics, Huazhong University of Science and Technology, Wuhan, China.|Department of Biomedical Engineering, School of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, China.|School of Mathematics and Statistics, Hubei University of Education, Wuhan, China.
authors.forename: Tingwei
authors.initials: T
authors.lastname: Quan
authors.affiliation: Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan, China.|Ministy of Education Key Laboratory for Biomedical Photonics, Huazhong University of Science and Technology, Wuhan, China.|Department of Biomedical Engineering, School of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: Hang
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan, China.|Ministy of Education Key Laboratory for Biomedical Photonics, Huazhong University of Science and Technology, Wuhan, China.|Department of Biomedical Engineering, School of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.affiliation: Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan, China.|Ministy of Education Key Laboratory for Biomedical Photonics, Huazhong University of Science and Technology, Wuhan, China.|Department of Biomedical Engineering, School of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: Shiwei
authors.initials: S
authors.lastname: Li
authors.affiliation: Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan, China.|Ministy of Education Key Laboratory for Biomedical Photonics, Huazhong University of Science and Technology, Wuhan, China.|Department of Biomedical Engineering, School of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: Anan
authors.initials: A
authors.lastname: Li
authors.affiliation: Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan, China.|Ministy of Education Key Laboratory for Biomedical Photonics, Huazhong University of Science and Technology, Wuhan, China.|Department of Biomedical Engineering, School of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: Yuxin
authors.initials: Y
authors.lastname: Li
authors.affiliation: Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan, China.|Ministy of Education Key Laboratory for Biomedical Photonics, Huazhong University of Science and Technology, Wuhan, China.|Department of Biomedical Engineering, School of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Lv
authors.affiliation: Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan, China.|Ministy of Education Key Laboratory for Biomedical Photonics, Huazhong University of Science and Technology, Wuhan, China.|Department of Biomedical Engineering, School of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: Qingming
authors.identifier: http://orcid.org/0000-0002-6725-9311
authors.initials: Q
authors.lastname: Luo
authors.affiliation: Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan, China.|Ministy of Education Key Laboratory for Biomedical Photonics, Huazhong University of Science and Technology, Wuhan, China.|Department of Biomedical Engineering, School of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Gong
authors.affiliation: Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan, China.|Ministy of Education Key Laboratory for Biomedical Photonics, Huazhong University of Science and Technology, Wuhan, China.|Department of Biomedical Engineering, School of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: Shaoqun
authors.initials: S
authors.lastname: Zeng
mesh_terms: D001331:Automation; D016501:Neurites; D009474:Neurons / Q000166:cytology*