title: A systematic review of observational studies on oxidative/nitrosative stress involvement in dengue pathogenesis.
abstract: OBJECTIVE
  Our objective was to systematically review the published observational research related to the role of oxidative-nitrosative stress in pathogenesis of dengue.
  
  
  METHODS
  We searched electronic databases (PubMed, EMBASE, The COCHRANE library, ScienceDirect, Scopus, SciELO, LILACS via Virtual Health Library, Google Scholar) using the term: dengue, dengue virus, severe dengue, oxidative stress, nitrosative stress, antioxidants, oxidants, free radicals, oxidized lipid products, lipid peroxides, nitric oxide, and nitric oxide synthase. Articles were selected for review by title and abstract excluding letter, review, in vivo and in vitro studies, and duplicates studies. Selected articles were reviewed for study design, original purposes, sample size, main outcomes, methods, and oxidative-nitrosative stress markers values.
  
  
  RESULTS
  In total, 4,331 non-duplicates articles were identified from electronic databases searches, of which 16 were eligible for full text searching. Data from the observational studies originate from Asian countries (50%; 8/16), South American countries (31.2%; 5/16), and Central America and the Caribbean countries (18.8%; 3/16). Case-control study was the type of design most common in researches reviewed. The 1997 World Health Organization (WHO) dengue case classification criteria were used in all studies included in this review.
  
  
  CONCLUSIONS
  Based on published data found in peer-reviewed literature, oxidative and nitrosative stress are demonstrated by changes in plasma levels of nitric oxide, antioxidants, lipid peroxidation and protein oxidation markers in patients with dengue infection. Additionally, elevated serum protein carbonyls and malondialdehyde levels appear to be associated with dengue disease severity.
authors.affiliation: University of San Buenaventura, Cartagena, Colombia . ; University of Cartagena, Cartagena, Colombia.
authors.forename: Raimundo
authors.initials: R
authors.lastname: Castro
authors.affiliation: Children's Hospital Foundation Napoleon Franco Pareja, Cartagena, Colombia.
authors.forename: Hernando Samuel
authors.initials: HS
authors.lastname: Pinzón
authors.affiliation: Children's Hospital Foundation Napoleon Franco Pareja, Cartagena, Colombia ; University of Cartagena, Cartagena, Colombia.
authors.forename: Nelson
authors.initials: N
authors.lastname: Alvis-Guzman
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D003715:Dengue / Q000503:physiopathology*; D006801:Humans; D015227:Lipid Peroxidation / Q000502:physiology; D009569:Nitric Oxide / Q000378:metabolism*; D018384:Oxidative Stress; D011506:Proteins / Q000378:metabolism; D012720:Severity of Illness Index
chemical_list: D000975:Antioxidants; D011506:Proteins; D009569:Nitric Oxide
keywords: Dengue; biological markers; dengue virus; nitrosative stress; oxidative stress; severe dengue; systematic review