title: Cubic Cu2O nanoframes with a unique edge-truncated structure and a good electrocatalytic activity for immunosensor application.
abstract: In this work, an ultrasensitive sandwich-type electrochemical immunosensor was developed for the quantitative detection of prostate specific antigen (PSA). Gold nanoparticles decorated 3-aminopropyltriethoxysilane functionalized graphene sheets (Au@APTES-GS) with a large specific surface area, good biocompatibility and superior electron transfer ability were employed as the matrix. In addition, cubic Cu2O nanoframes with hollow edges were employed as the label for the first time. Scanning electron microscope (SEM) images were used to confirm the nanostructure of Au@APTES-GS and Cu2O. Using square wave voltammetry (SWV) to monitor the electrocatalytic process, the signal amplification mechanism of the matrix and the label were explored successfully. Here we find that the unique edge-truncated structure of Cu2O nanoframes can load with a larger amount of redox mediators, ferrocenecarboxylic acid (Fc-COOH), offering a higher electrochemical signal response. Apart from that, Cu2O nanoframes have a good electrocatalytic activity towards the Fc-COOH and hydrogen peroxide (H2O2), making a contribution to further enhance the sensitivity of the fabricated immunosensor. Under optimal conditions, the proposed immunosensor achieved an ultrasensitive and specific detection of PSA, and displayed acceptable reproducibility, selectivity and stability. This work may provide an effective method for the clinical monitoring of tumor markers and demonstrate the potential application promising of nanoframes in the fabrication of immunosensors.
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
authors.forename: Hongmin
authors.initials: H
authors.lastname: Ma
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
authors.forename: Yulan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
authors.forename: Lihua
authors.initials: L
authors.lastname: Hu
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
authors.forename: Dawei
authors.initials: D
authors.lastname: Fan
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
authors.forename: Tao
authors.initials: T
authors.lastname: Yan
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China. Electronic address: sdjndxwq@163.com.
authors.forename: Qin
authors.initials: Q
authors.lastname: Wei
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry / Q000276:immunology; D014408:Biomarkers, Tumor / Q000276:immunology / Q000302:isolation & purification*; D015374:Biosensing Techniques; D003300:Copper / Q000737:chemistry; D055664:Electrochemical Techniques / Q000379:methods*; D006046:Gold / Q000737:chemistry; D006108:Graphite / Q000737:chemistry; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D007118:Immunoassay; D053768:Metal Nanoparticles / Q000737:chemistry; D017430:Prostate-Specific Antigen / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D055910:Antibodies, Immobilized; D014408:Biomarkers, Tumor; D006046:Gold; D006108:Graphite; D003300:Copper; D006861:Hydrogen Peroxide; D017430:Prostate-Specific Antigen; C000520:cuprous oxide
keywords: Electrocatalytic activity; Ferrocenecarboxylic acid; Immunosensor; Nanoframes; Prostate specific antigen