title: A Hybrid Semi-Digital Transimpedance Amplifier With Noise Cancellation Technique for Nanopore-Based DNA Sequencing.
abstract: Over the past two decades, nanopores have been a promising technology for next generation deoxyribonucleic acid (DNA) sequencing. Here, we present a hybrid semi-digital transimpedance amplifier (HSD-TIA) to sense the minute current signatures introduced by single-stranded DNA (ssDNA) translocating through a nanopore, while discharging the baseline current using a semi-digital feedback loop. The amplifier achieves fast settling by adaptively tuning a DC compensation current when a step input is detected. A noise cancellation technique reduces the total input-referred current noise caused by the parasitic input capacitance. Measurement results show the performance of the amplifier with 31.6 M Ω mid-band gain, 950 kHz bandwidth, and 8.5 fA/ √Hz input-referred current noise, a 2× noise reduction due to the noise cancellation technique. The settling response is demonstrated by observing the insertion of a protein nanopore in a lipid bilayer. Using the nanopore, the HSD-TIA was able to measure ssDNA translocation events.
authors.forename: Chung-Lun
authors.initials: CL
authors.lastname: Hsu
authors.forename: Haowei
authors.initials: H
authors.lastname: Jiang
authors.forename: A G
authors.initials: AG
authors.lastname: Venkatesh
authors.forename: Drew A
authors.initials: DA
authors.lastname: Hall
mesh_terms: D001698:Biomedical Engineering / Q000295:instrumentation; D004247:DNA / Q000032:analysis / Q000737:chemistry* / Q000235:genetics; D004867:Equipment Design; D008051:Lipid Bilayers / Q000737:chemistry; D058608:Nanopores; D017422:Sequence Analysis, DNA / Q000295:instrumentation*; D012815:Signal Processing, Computer-Assisted / Q000295:instrumentation*
chemical_list: D008051:Lipid Bilayers; D004247:DNA