title: Zscan10 is dispensable for maintenance of pluripotency in mouse embryonic stem cells.
abstract: Zinc finger and SCAN domain-containing 10 (Zscan10, also known as Zfp206) encodes a transcription factor that has been reported to be involved in the maintenance of pluripotency in mouse embryonic stem (ES) cells. Here we generated inducible knockout ES cells for Zscan10 using the Cre-loxP system and analyzed its function. We succeeded in establishing Zscan10-null ES cells and confirmed their pluripotency by the generation of chimeric embryos. Our results clearly indicate that Zscan10 is dispensable for the ability of self-renewal and differentiation in ES cells.
authors.affiliation: Laboratory for Pluripotent Stem Cell Studies, RIKEN Center for Developmental Biology (CDB), 2-2-3 Minatojima-minamimachi, Chuo-ku, Kobe 650-0047, Japan.
authors.forename: Mariko
authors.initials: M
authors.lastname: Yamane
authors.affiliation: Laboratory for Pluripotent Stem Cell Studies, RIKEN Center for Developmental Biology (CDB), 2-2-3 Minatojima-minamimachi, Chuo-ku, Kobe 650-0047, Japan.
authors.forename: Setsuko
authors.initials: S
authors.lastname: Fujii
authors.affiliation: Laboratory for Pluripotent Stem Cell Studies, RIKEN Center for Developmental Biology (CDB), 2-2-3 Minatojima-minamimachi, Chuo-ku, Kobe 650-0047, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Ohtsuka
authors.affiliation: Laboratory for Pluripotent Stem Cell Studies, RIKEN Center for Developmental Biology (CDB), 2-2-3 Minatojima-minamimachi, Chuo-ku, Kobe 650-0047, Japan; Laboratory for Development and Regenerative Medicine, Kobe University Graduate School of Medicine, 7-5-1 Kusunokicho, Chuo-ku, Kobe 650-0017, Japan; JST, CREST, Sanbancho, Chiyoda-ku, Tokyo 102-0075, Japan; Department of Pluripotent Stem Cell Biology, Institute of Molecular Embryology and Genetics (IMEG), 2-2-1 Honjo, Chuo-ku, Kumamoto 860-0811, Japan. Electronic address: niwa@cdb.riken.jp.
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Niwa
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D049109:Cell Proliferation / Q000502:physiology; D002478:Cells, Cultured; D051379:Mice; D000066450:Mouse Embryonic Stem Cells / Q000166:cytology* / Q000502:physiology*; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000502:physiology*; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D014157:Transcription Factors; C514588:Zscan10 protein, mouse
keywords: Embryonic stem cells; Pluripotency; Zscan10