title: Effect of Quencher, Denaturants, Temperature and pH on the Fluorescent Properties of BSA Protected Gold Nanoclusters.
abstract: In this paper, we have synthesized BSA protected gold nanoclusters (BSA Au nanocluster) and studied the effect of quencher, protein denaturant, pH and temperature on the fluorescence properties of the tryptophan molecule of the BSA Au nanocluster and native BSA. We have also studied their effect on the peak emission of BSA Au nanoclusters (650 nm). The phtophysical characterization of a newly developed fluorophore in different environments is absolutely necessary to futher develop their biomedical and analytical applications. It was observed from our experiments that the tryptophan in BSA Au nanoclusters is better shielded from the polar environment. Tryptophan in native BSA showed a red shift in its peak emission wavelength position. Tryptophan is a highly polarity sensitive dye and a minimal change in its microenvironment can be easily observed in its photophysical properties.
authors.affiliation: Department of Cell Biology and Immunology, Center for Fluorescence Technologies and Nanomedicine, University of North Texas Health Science Center, Fort Worth, TX, 76107,USA.
authors.forename: Rahul
authors.initials: R
authors.lastname: Chib
authors.affiliation: Department of Cell Biology and Immunology, Center for Fluorescence Technologies and Nanomedicine, University of North Texas Health Science Center, Fort Worth, TX, 76107,USA.
authors.forename: Susan
authors.initials: S
authors.lastname: Butler
authors.affiliation: Department of Cell Biology and Immunology, Center for Fluorescence Technologies and Nanomedicine, University of North Texas Health Science Center, Fort Worth, TX, 76107,USA ; Department of Physics and Astronomy, Texas Christian University, Fort Worth, TX, 76129, USA.
authors.forename: Sangram
authors.initials: S
authors.lastname: Raut
authors.affiliation: Department of Cell Biology and Immunology, Center for Fluorescence Technologies and Nanomedicine, University of North Texas Health Science Center, Fort Worth, TX, 76107,USA.
authors.forename: Sunil
authors.initials: S
authors.lastname: Shah
authors.affiliation: Department of Cell Biology and Immunology, Center for Fluorescence Technologies and Nanomedicine, University of North Texas Health Science Center, Fort Worth, TX, 76107,USA.
authors.forename: Julian
authors.initials: J
authors.lastname: Borejdo
authors.affiliation: Department of Cell Biology and Immunology, Center for Fluorescence Technologies and Nanomedicine, University of North Texas Health Science Center, Fort Worth, TX, 76107,USA ; Department of Physics and Astronomy, Texas Christian University, Fort Worth, TX, 76129, USA.
authors.forename: Zygmunt
authors.initials: Z
authors.lastname: Gryczynski
authors.affiliation: Department of Cell Biology and Immunology, Center for Fluorescence Technologies and Nanomedicine, University of North Texas Health Science Center, Fort Worth, TX, 76107,USA.
authors.forename: Ignacy
authors.initials: I
authors.lastname: Gryczynski
keywords: BSA Au nanoclusters; denaturants; fluorescence; quenching; tryptophan emission