title: Bioactive minor components of the total salvianolic acids injection prepared from Salvia miltiorrhiza Bge.
abstract: The total salvianolic acids are main effective constituents of Salvia miltiorrhiza Bge., a traditional Chinese medicine used for thousands of years. The purpose of present study was to make clear the composition and bioactivities of the minor components of the total salvianolic acids injection. As a result, three new minor phenolic acids (1-3) together with six known compounds (4-9) were characterized from the total salvianolic acids injection. Their structures were elucidated by extensive analysis of the spectral data. The absolute configuration of compounds 1-3 were confirmed by their J7',8' observed in (1)H NMR spectra, absorption band at approximately 250-260nm in their CD spectra as well as chemical shifts of C-8″ and C-8‴ displayed in (13)C NMR spectra. Then DPPH free radical scavenging assay and
  quinine oxidoreductase 1 (NQO1) inducing activity test were employed to evaluate the antioxidant effect of new minor compounds 1 and 2. Compound 2 showed significant NQO1 inducing activity at 20μM with IR value 2.6. Meanwhile, DPPH scavenging assay revealed that the inhibition rates of compounds 1 and 2 were 84.3% and 74.9% at 2mM, respectively.
authors.affiliation: School of Traditional Chinese Materia Media, Shenyang Pharmaceutical University, Shenyang 110016, China; Key Laboratory of Structure-Based Drug Design and Discovery (Shenyang Pharmaceutical University), Ministry of Education, Wenhua Road 103, Shenyang 110016, China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Si
authors.affiliation: School of Traditional Chinese Materia Media, Shenyang Pharmaceutical University, Shenyang 110016, China; Key Laboratory of Structure-Based Drug Design and Discovery (Shenyang Pharmaceutical University), Ministry of Education, Wenhua Road 103, Shenyang 110016, China. Electronic address: liningsypharm@163.com.
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.affiliation: Tasly R&D Institute, Tianjin Tasly Group Co., Ltd., Tianjin 300402, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Tong
authors.affiliation: Key Laboratory of Structure-Based Drug Design and Discovery (Shenyang Pharmaceutical University), Ministry of Education, Wenhua Road 103, Shenyang 110016, China; School of Pharmaceutical Engineering, Shenyang Pharmaceutical University, Shenyang 110016, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Lin
authors.affiliation: School of Traditional Chinese Materia Media, Shenyang Pharmaceutical University, Shenyang 110016, China; Key Laboratory of Structure-Based Drug Design and Discovery (Shenyang Pharmaceutical University), Ministry of Education, Wenhua Road 103, Shenyang 110016, China.
authors.forename: Wenli
authors.initials: W
authors.lastname: Wang
authors.affiliation: School of Traditional Chinese Materia Media, Shenyang Pharmaceutical University, Shenyang 110016, China; Key Laboratory of Structure-Based Drug Design and Discovery (Shenyang Pharmaceutical University), Ministry of Education, Wenhua Road 103, Shenyang 110016, China.
authors.forename: Yachao
authors.initials: Y
authors.lastname: Xing
authors.affiliation: Tasly R&D Institute, Tianjin Tasly Group Co., Ltd., Tianjin 300402, China.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Liu
authors.affiliation: Tianjin Tasly Pride Pharmaceutical Co., Ltd., Tianjin 300402, China.
authors.forename: Hongshui
authors.initials: H
authors.lastname: Yue
authors.affiliation: Tasly R&D Institute, Tianjin Tasly Group Co., Ltd., Tianjin 300402, China.
authors.forename: Jingyao
authors.initials: J
authors.lastname: Xu
authors.affiliation: Tianjin Tasly Pride Pharmaceutical Co., Ltd., Tianjin 300402, China.
authors.forename: Aichun
authors.initials: A
authors.lastname: Ju
mesh_terms: D000475:Alkenes / Q000737:chemistry*; D066241:Carbon-13 Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D059808:Polyphenols / Q000737:chemistry*; D066244:Proton Magnetic Resonance Spectroscopy; D027561:Salvia miltiorrhiza / Q000737:chemistry*
chemical_list: D000475:Alkenes; D059808:Polyphenols; C568740:salvianolic acid
keywords: Bioactive minor components; DPPH scavenging activity; NQO1 inducing activity; Salvia miltiorrhiza Bge.; The total salvianolic acids injection