title: Effects of electromagnetic fields on the metabolism of lubricin of rat chondrocytes.
abstract: Electromagnetic fields (EMFs) can improve pain, stiffness and physical function in osteoarthritis (OA) patients and have been proposed for the treatment of OA. However, the precise mechanisms involved in this process are still not fully understood. In the present study, we investigated the effects of exposure for different durations with 75 Hz, 2.3 mT sinusoidal EMFs (SEMFs) on the metabolism of lubricin of rat chondrocytes cultured in vitro. Our results showed that SEMFs exposure promoted lubricin synthesis in a time-dependent manner, and the expression of transforming growth factor (TGF)-β1 was also enhanced after SEMFs treatment. The up-regulation effect of the expression of lubricin under SEMF was partly reduced by SB431542, an inhibitor of TGF-RI kinase. The Smad pathway was also investigated in our study. Smad2 synthesis was higher in EMF-exposed condition than in controls, whereas no effects were observed on inhibitory Smads (Smad6 and Smad7) production. Altogether, these data suggest that SEMF exposure can promote lubricin synthesis of rat chondrocytes in a time-dependent manner and that the TGF-β/Smads signaling pathway plays a partial role.
authors.affiliation: a Department of Orthopedics, Tongji Hospital , Tongji Medical College, Huazhong University of Science and Technology , Wuhan , China.|b Department of Orthopedics, WuHan Orthopedics Hospital/Puai Hospital , Tongji Medical College, Huazhong University of Science and Technology , Wuhan , China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: a Department of Orthopedics, Tongji Hospital , Tongji Medical College, Huazhong University of Science and Technology , Wuhan , China.
authors.forename: Wenkai
authors.initials: W
authors.lastname: Li
authors.affiliation: a Department of Orthopedics, Tongji Hospital , Tongji Medical College, Huazhong University of Science and Technology , Wuhan , China.
authors.forename: Mingyu
authors.initials: M
authors.lastname: Song
authors.affiliation: a Department of Orthopedics, Tongji Hospital , Tongji Medical College, Huazhong University of Science and Technology , Wuhan , China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Wei
authors.affiliation: a Department of Orthopedics, Tongji Hospital , Tongji Medical College, Huazhong University of Science and Technology , Wuhan , China.
authors.forename: Chaoxu
authors.initials: C
authors.lastname: Liu
authors.affiliation: a Department of Orthopedics, Tongji Hospital , Tongji Medical College, Huazhong University of Science and Technology , Wuhan , China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Yang
authors.affiliation: a Department of Orthopedics, Tongji Hospital , Tongji Medical College, Huazhong University of Science and Technology , Wuhan , China.
authors.forename: Hua
authors.initials: H
authors.lastname: Wu
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D019902:Chondrocytes / Q000378:metabolism*; D004574:Electromagnetic Fields; D005786:Gene Expression Regulation; D006023:Glycoproteins / Q000378:metabolism*; D008297:Male; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D000077293:Receptor, Transforming Growth Factor-beta Type I; D018125:Receptors, Transforming Growth Factor beta / Q000378:metabolism; D015398:Signal Transduction; D051785:Smad Proteins / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000378:metabolism; D000099158:Proteoglycan 4
chemical_list: D006023:Glycoproteins; D017346:Protein Serine-Threonine Kinases; D012333:RNA, Messenger; D000077293:Receptor, Transforming Growth Factor-beta Type I; D018125:Receptors, Transforming Growth Factor beta; D051785:Smad Proteins; D016212:Transforming Growth Factor beta; D000099158:Proteoglycan 4; C000625843:Tgfbr1 protein, rat
keywords: Chondrocytes; Osteoarthritis (OA); TGF-β/Smads signaling pathway; lubricin; sinusoidal electromagnetic fields (SEMF)