title: The effects of hierarchical micro/nanosurfaces decorated with TiO2 nanotubes on the bioactivity of titanium implants in vitro and in vivo.
abstract: In the present work, a hierarchical hybrid micro/nanostructured titanium surface was obtained by sandblasting with large grit and acid etching (SLA), and nanotubes of different diameters (30 nm, 50 nm, and 80 nm) were superimposed by anodization. The effect of each SLA-treated surface decorated with nanotubes (SLA + 30 nm, SLA + 50 nm, and SLA + 80 nm) on osteogenesis was studied in vitro and in vivo. The human MG63 osteosarcoma cell line was used for cytocompatibility evaluation, which showed that cell adhesion and proliferation were dramatically enhanced on SLA + 30 nm. In comparison with cells grown on the other tested surfaces, those grown on SLA + 80 nm showed an enhanced expression of osteogenesis-related genes. Cell spread was also enhanced on SLA + 80 nm. A canine model was used for in vivo evaluation of bone bonding. Histological examination demonstrated that new bone was formed more rapidly on SLA-treated surfaces with nanotubes (especially SLA + 80 nm) than on those without nanotubes. All of these results indicate that SLA + 80 nm is favorable for promoting the activity of osteoblasts and early bone bonding.
authors.affiliation: Center of Oral Implantology, Guangdong Provincial Stomatological Hospital, Southern Medical University, Guangzhou, People's Republic of China.
authors.forename: Xianglong
authors.initials: X
authors.lastname: Ding
authors.affiliation: Center of Oral Implantology, Guangdong Provincial Stomatological Hospital, Southern Medical University, Guangzhou, People's Republic of China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Department of Stomatology, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, People's Republic of China.
authors.forename: Jingxu
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Stomatology, Nanfang Hospital, Southern Medical University, Guangzhou, People's Republic of China.
authors.forename: Qingxia
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: Center of Oral Implantology, Guangdong Provincial Stomatological Hospital, Southern Medical University, Guangzhou, People's Republic of China.
authors.forename: Xi
authors.initials: X
authors.lastname: Lin
authors.affiliation: Center of Oral Implantology, Guangdong Provincial Stomatological Hospital, Southern Medical University, Guangzhou, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Department of Periodontics and Implantology, Guangdong Provincial Stomatological Hospital, Southern Medical University, Guangzhou, People's Republic of China.
authors.forename: Shaobing
authors.initials: S
authors.lastname: Li
authors.affiliation: Center of Oral Implantology, Guangdong Provincial Stomatological Hospital, Southern Medical University, Guangzhou, People's Republic of China.
authors.forename: Jingyi
authors.initials: J
authors.lastname: Wu
authors.affiliation: Department of Periodontics and Implantology, Guangdong Provincial Stomatological Hospital, Southern Medical University, Guangzhou, People's Republic of China.
authors.forename: Mingdeng
authors.initials: M
authors.lastname: Rong
authors.affiliation: Center of Oral Implantology, Guangdong Provincial Stomatological Hospital, Southern Medical University, Guangzhou, People's Republic of China.
authors.forename: Zehong
authors.initials: Z
authors.lastname: Guo
authors.affiliation: Center of Oral Implantology, Guangdong Provincial Stomatological Hospital, Southern Medical University, Guangzhou, People's Republic of China.
authors.forename: Chunhua
authors.initials: C
authors.lastname: Lai
authors.affiliation: Department of Periodontics and Implantology, Guangdong Provincial Stomatological Hospital, Southern Medical University, Guangzhou, People's Republic of China.
authors.forename: Haibin
authors.initials: H
authors.lastname: Lu
authors.affiliation: Center of Oral Implantology, Guangdong Provincial Stomatological Hospital, Southern Medical University, Guangzhou, People's Republic of China.
authors.forename: Fang
authors.initials: F
authors.lastname: Jia
mesh_terms: D000327:Adsorption; D000818:Animals; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D004285:Dogs; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D008297:Male; D043942:Nanotubes / Q000737:chemistry* / Q000648:ultrastructure; D016348:Osseointegration; D019736:Prostheses and Implants; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013499:Surface Properties; D014025:Titanium / Q000494:pharmacology*; D014867:Water
chemical_list: D012333:RNA, Messenger; D014867:Water; C009495:titanium dioxide; D014025:Titanium
keywords: dental and orthopedic implant; nanotopography; osseointegration; titanium