title: Down-regulation of CD53 expression in Epinephelus coioides under LPS, poly (I:C), and cytokine stimulation.
abstract: Tetraspanins are a group of cell surface molecules involved in cell adhesion, motility, metastasis, signal transduction, and immune cell activation. Members of the tetraspanin family include CD9, CD37, CD63, CD53, and others. However, few tetraspanins have been investigated in teleosts. In this study, we obtained the open reading frame of CD53 cDNA from orange spotted grouper (Epinephelus coioices), an economically important fish. The predicted amino acid structure contains four membrane-spanning domains and a conserved CCG motif. The amino acid identity between human and grouper CD53 was only 38%; however, both CD53 proteins share the same structure. Quantitative real-time PCR revealed that mRNA is abundant in immune organs, including the head and trunk kidneys, spleen, thymus, gill, and blood. Immunochemistry and immunofluorescence analyses further revealed that CD53 was majorly expressed in the leukocytes of various organs. Finally, mRNA and protein expression for CD53 was down-regulated in fish treated with immune stimulators, including LPS, Poly (I:C), Vibrio, recombinant grouper IL-6, and CCL4. Our results indicate that the expression of CD53 may play important roles in pathogen invasion and inflammation reaction.
authors.affiliation: Institute of Biotechnology, College of Bioscience and Biotechnology, National Cheng Kung University, Tainan 701, Taiwan; Chi Mei Medical Center, Liouying, No.201, Taikang, Liuying Dist., Tainan City 736, Taiwan.
authors.forename: Chia-Yi
authors.initials: CY
authors.lastname: Hou
authors.affiliation: Institute of Biotechnology, College of Bioscience and Biotechnology, National Cheng Kung University, Tainan 701, Taiwan; Department of Biotechnology and Bioindustry Sciences, National Cheng Kung University, Tainan 701, Taiwan; Research Center of Agricultural Biotechnology, National Cheng Kung University, Tainan 701, Taiwan; Center of Biosciences, National Cheng Kung University, Tainan 701, Taiwan.
authors.forename: John Han-You
authors.initials: JH
authors.lastname: Lin
authors.affiliation: Institute of Biotechnology, College of Bioscience and Biotechnology, National Cheng Kung University, Tainan 701, Taiwan.
authors.forename: Shih-Jie
authors.initials: SJ
authors.lastname: Lin
authors.affiliation: Institute of Biotechnology, College of Bioscience and Biotechnology, National Cheng Kung University, Tainan 701, Taiwan.
authors.forename: Wan-Ching
authors.initials: WC
authors.lastname: Kuo
authors.affiliation: Department of Biotechnology, Ming Chuan University, Taoyuan County 333, Taiwan. Electronic address: htlin@mail.mcu.edu.tw.
authors.forename: Han-Tso
authors.initials: HT
authors.lastname: Lin
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001492:Bass / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism; D016207:Cytokines / Q000494:pharmacology; D015536:Down-Regulation; D029941:Fish Proteins / Q000235:genetics* / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D010802:Phylogeny; D011070:Poly I-C / Q000494:pharmacology; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment / Q000662:veterinary; D060265:Tetraspanin 25 / Q000235:genetics* / Q000378:metabolism; D014733:Vibrio / Q000502:physiology
chemical_list: D016207:Cytokines; D029941:Fish Proteins; D008070:Lipopolysaccharides; D060265:Tetraspanin 25; D011070:Poly I-C
keywords: CD53; Epinephelus coioides; LPS; Poly (I:C); Tetraspanin