title: Visualization of Bacterial Microcompartment Facet Assembly Using High-Speed Atomic Force Microscopy.
abstract: Bacterial microcompartments (BMCs) are proteinaceous organelles widespread among bacterial phyla. They compartmentalize enzymes within a selectively permeable shell and play important roles in CO2 fixation, pathogenesis, and microbial ecology. Here, we combine X-ray crystallography and high-speed atomic force microscopy to characterize, at molecular resolution, the structure and dynamics of BMC shell facet assembly. Our results show that preformed hexamers assemble into uniformly oriented shell layers, a single hexamer thick. We also observe the dynamic process of shell facet assembly. Shell hexamers can dissociate from and incorporate into assembled sheets, indicating a flexible intermolecular interaction. Furthermore, we demonstrate that the self-assembly and dynamics of shell proteins are governed by specific contacts at the interfaces of shell proteins. Our study provides novel insights into the formation, interactions, and dynamics of BMC shell facets, which are essential for the design and engineering of self-assembled biological nanoreactors and scaffolds based on BMC architectures.
authors.affiliation: MSU-DOE Plant Research Laboratory, Michigan State University , East Lansing, Michigan 48824, United States.|Physical Biosciences Division, Lawrence Berkeley National Laboratory , Berkeley, California 94720, United States.
authors.forename: Markus
authors.initials: M
authors.lastname: Sutter
authors.forename: Matthew
authors.initials: M
authors.lastname: Faulkner
authors.affiliation: MSU-DOE Plant Research Laboratory, Michigan State University , East Lansing, Michigan 48824, United States.
authors.forename: Clément
authors.initials: C
authors.lastname: Aussignargues
authors.affiliation: MSU-DOE Plant Research Laboratory, Michigan State University , East Lansing, Michigan 48824, United States.
authors.forename: Bradley C
authors.initials: BC
authors.lastname: Paasch
authors.forename: Steve
authors.initials: S
authors.lastname: Barrett
authors.affiliation: MSU-DOE Plant Research Laboratory, Michigan State University , East Lansing, Michigan 48824, United States.|Physical Biosciences Division, Lawrence Berkeley National Laboratory , Berkeley, California 94720, United States.|Department of Plant and Microbial Biology, University of California, Berkeley , Berkeley, California 94720, United States.|Berkeley Synthetic Biology Institute , Berkeley, California 94720, United States.|Department of Biochemistry and Molecular Biology, Michigan State University , East Lansing, Michigan 48824, United States.
authors.forename: Cheryl A
authors.initials: CA
authors.lastname: Kerfeld
authors.forename: Lu-Ning
authors.initials: LN
authors.lastname: Liu
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis / Q000235:genetics / Q000648:ultrastructure*; D018360:Crystallography, X-Ray; D018625:Microscopy, Atomic Force / Q000379:methods*; D009231:Myxococcales / Q000166:cytology* / Q000235:genetics / Q000648:ultrastructure; D017354:Point Mutation; D011487:Protein Conformation
chemical_list: D001426:Bacterial Proteins
keywords: Bacterial microcompartment; high-speed atomic force microscopy; protein dynamics; protein interaction; self-assembly