title: Identification of Central Nervous System Proteins in Human Blood Serum and Plasma.
abstract: Mass-spectrometric identification of proteins in human blood plasma and serum was performed by comparing mass-spectra of fragmented peptides using Swiss-Prot and UniProtKB databases of amino acid sequences. After choosing the appropriate identification conditions we found that combination of spectrum search parameters are optimal for identification of CNS proteins. In the studied plasma and serum samples, 9 proteins involved into pathological processes in the nervous tissue were identified; 7 of them were identified in both plasma and serum.
authors.affiliation: V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia. yuliana.miroshnichenko@gmail.com.
authors.forename: Yu V
authors.initials: YV
authors.lastname: Miroshnichenko
authors.affiliation: V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia.
authors.forename: N A
authors.initials: NA
authors.lastname: Petushkova
authors.affiliation: N. N. Burdenko Research Institute of Neurosurgery, Russian Academy of Medical Sciences, Moscow, Russia.
authors.forename: N B
authors.initials: NB
authors.lastname: Teryaeva
authors.affiliation: V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia.
authors.forename: A V
authors.initials: AV
authors.lastname: Lisitsa
authors.affiliation: V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia.
authors.forename: V G
authors.initials: VG
authors.lastname: Zgoda
authors.affiliation: V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia.
authors.forename: A Yu
authors.initials: AY
authors.lastname: Belyaev
authors.affiliation: N. N. Burdenko Research Institute of Neurosurgery, Russian Academy of Medical Sciences, Moscow, Russia.
authors.forename: A A
authors.initials: AA
authors.lastname: Potapov
mesh_terms: D000328:Adult; D015415:Biomarkers; D002851:Chromatography, High Pressure Liquid; D030562:Databases, Protein; D005260:Female; D006801:Humans; D009419:Nerve Tissue Proteins / Q000097:blood*; D010949:Plasma / Q000737:chemistry; D012016:Reference Values; D044967:Serum / Q000737:chemistry; D053719:Tandem Mass Spectrometry
chemical_list: D015415:Biomarkers; D009419:Nerve Tissue Proteins
keywords: brain damage; neurodegenerative diseases; pathology of the central nervous system; protein identification; proteomics