title: Toxicity and Antiviral Activity of the Extracts of Submerged Mycelium of Nematophagous Duddingtonia flagrans Fungus in Vero Cell Culture.
abstract: We studied toxicity and antiviral activity of aqueous and ethanol extracts of bioactive substances from the biomass of nematophagous fungus Duddingtonia flagrans prepared by submerged culturing of the mycelium. It is found that both extracts were characterized by low toxicity for cultured Vero cells and inhibited reproduction of DNA-viruses in this cell line. Ethanol extract of the fungus exhibited higher in vitro antiviral activity against Herpes simplex virus type 2, ectromelia virus, and vaccinia virus than water extract, which can be due to higher content of proteins, polysaccharides, flavonols, catechins, or carotenes or more effective their combination. The extracts of cultured mycelium of Duddingtonia flagrans fungus containing a complex of bioactive substances can be used for creation of broad-spectrum antiviral drugs against DNA-viruses.
authors.affiliation: Vector State Research Center of Virology and Biotechnology, Koltsovo, Novosibirsk Region, Russia. bragimova@vector.nsc.ru.
authors.forename: Zh B
authors.initials: ZhB
authors.lastname: Ibragimova
authors.affiliation: Vector State Research Center of Virology and Biotechnology, Koltsovo, Novosibirsk Region, Russia.
authors.forename: G G
authors.initials: GG
authors.lastname: Anan'ko
authors.affiliation: Vector State Research Center of Virology and Biotechnology, Koltsovo, Novosibirsk Region, Russia.
authors.forename: N E
authors.initials: NE
authors.lastname: Kostina
authors.affiliation: Vector State Research Center of Virology and Biotechnology, Koltsovo, Novosibirsk Region, Russia.
authors.forename: T V
authors.initials: TV
authors.lastname: Teplyakova
authors.affiliation: Vector State Research Center of Virology and Biotechnology, Koltsovo, Novosibirsk Region, Russia.
authors.forename: N A
authors.initials: NA
authors.lastname: Mazurkova
mesh_terms: D000818:Animals; D002522:Chlorocebus aethiops; D059586:Duddingtonia / Q000737:chemistry*; D004481:Ectromelia virus / Q000187:drug effects; D025282:Mycelium / Q000737:chemistry*; D018139:Simplexvirus / Q000187:drug effects; D014616:Vaccinia virus / Q000187:drug effects; D014709:Vero Cells
keywords: herpes simplex virus; nematophagous fungus extract; orthopoxviruses