title: The mitotic regulator Hec1 is a critical modulator of prostate cancer through the long non-coding RNA BX647187 in vitro.
abstract: Hec1 (highly expressed in cancer) is a member of a conserved Ndc80 (nuclear division cycle 80) complex that regulates mitotic processes. Its overexpression is seen in various tumours and is associated with cancer progression. However, its expression pattern and role inhuman prostate cancer (PCa) still not clear. The aim of our study is to investigate the expression and functional role of Hec1 in human PCa. Hec1 expression was measured in 10 pairs of PCa cancerous and non-cancerous tissue samples by quantitative real-time (qRT)-PCR. The effects of Hec1 on PCa cells were studied by RNAi approach. Apoptosis and cell cycle were analysed by flow cytometry. Cells viability was evaluated using cell counting Kit-8. Cyclin B1-Cdc2 (cell division cycle 2) activity was measured by ELISA assay. Long non-coding (Lnc)RNAs regulated by Hec1 were gained from bioinformatics analysis. The role of LncRNA BX647187, regulated by Hec1, was finally characterized in PCa cells by siRNA. Our results showed that Hec1 mRNA and protein were significantly overexpressed in Human PCa tissues and several PCa cell lines. Silencing Hec1 markedly suppressed proliferation, promoted apoptosis and induced cell-cycle arrest in G2/M-phase in PCa cells. Through bioinformatics analysis and knockdown Hec1 in PCa cells, we found LncRNA BX647187 was positively regulated by Hec1. We further demonstrated that suppression of BX647187 in PCa cells significantly reduced cell proliferation and promoted apoptosis. Thus, we conclude that Hec1 is consistently overexpressed in human PCa and Hec1 is closely linked with human PCa progression through the meditator LncRNA BX647187. Our studies may contribute to understand the molecular mechanism of PCa pathogenesis and clinical therapy.
authors.affiliation: Department of Urology, Changhai Hospital, Second Military University, Shanghai, 20043, China.
authors.forename: Haifeng
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Urology, Changhai Hospital, Second Military University, Shanghai, 20043, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Gao
authors.affiliation: Department of Urology, Changhai Hospital, Second Military University, Shanghai, 20043, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Lu
authors.affiliation: Department of Urology, Changhai Hospital, Second Military University, Shanghai, 20043, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Urology, Changhai Hospital, Second Military University, Shanghai, 20043, China.
authors.forename: Chunfei
authors.initials: C
authors.lastname: Ma
authors.affiliation: Department of Urology, Changhai Hospital, Second Military University, Shanghai, 20043, China.
authors.forename: Zhenkai
authors.initials: Z
authors.lastname: Shi
authors.affiliation: Department of Urology, Changhai Hospital, Second Military University, Shanghai, 20043, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Zhu
authors.affiliation: Department of Urology, Changhai Hospital, Second Military University, Shanghai, 20043, China.
authors.forename: Biming
authors.initials: B
authors.lastname: He
authors.affiliation: Department of Urology, Changhai Hospital, Second Military University, Shanghai, 20043, China.
authors.forename: Chuanliang
authors.initials: C
authors.lastname: Xu
authors.affiliation: Department of Urology, Changhai Hospital, Second Military University, Shanghai, 20043, China yinghaosun1@163.com.
authors.forename: Yinghao
authors.initials: Y
authors.lastname: Sun
mesh_terms: D017209:Apoptosis / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics*; D002470:Cell Survival / Q000235:genetics; D003598:Cytoskeletal Proteins; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D006801:Humans; D008297:Male; D009687:Nuclear Proteins / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000235:genetics*; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology; D062085:RNA, Long Noncoding / Q000235:genetics*
chemical_list: D003598:Cytoskeletal Proteins; C120529:NDC80 protein, human; D009687:Nuclear Proteins; D062085:RNA, Long Noncoding; C000610362:long non-coding RNA BX647187, human
keywords: Hec1; apoptosis; bioinformatics analysis; cell cycle; human prostate cancer; long non-coding RNA BX647187