title: Improved in situ saccharification of cellulose pretreated by dimethyl sulfoxide/ionic liquid using cellulase from a newly isolated Paenibacillus sp. LLZ1.
abstract: A cellulase producing strain was newly isolated from soil samples and identified as Paenibacillus sp. LLZ1. A novel aqueous-dimethyl sulfoxide (DMSO)/1-ethyl-3-methylimidazolium diethyl phosphate ([Emin]DEP)-cellulase system was designed and optimized. In the pretreatment, DMSO was found to be a low-cost substitute of up to 70% ionic liquid to enhance the cellulose dissolution. In the enzymatic saccharification, the optimum pH and temperature of the Paenibacillus sp. LLZ1 cellulase were identified as 6.0 and 40°C, respectively. Under the optimized reaction condition, the conversion of microcrystalline cellulose and bagasse cellulose increased by 39.3% and 37.6%, compared with unpretreated cellulose. Compared to current methods of saccharification, this new approach has several advantages including lower operating temperature, milder pH, and less usage of ionic liquid, indicating a marked progress in environmental friendly hydrolysis of biomass-based materials.
authors.affiliation: School of Chemistry, Biology, and Material Engineering, Suzhou University of Science and Technology, Suzhou 215009, PR China.
authors.forename: Dongxue
authors.initials: D
authors.lastname: Hu
authors.affiliation: School of Chemistry, Biology, and Material Engineering, Suzhou University of Science and Technology, Suzhou 215009, PR China.
authors.forename: Xin
authors.initials: X
authors.lastname: Ju
authors.affiliation: School of Chemistry, Biology, and Material Engineering, Suzhou University of Science and Technology, Suzhou 215009, PR China. Electronic address: liliangzhi01@163.com.
authors.forename: Liangzhi
authors.initials: L
authors.lastname: Li
authors.affiliation: School of Chemistry, Biology, and Material Engineering, Suzhou University of Science and Technology, Suzhou 215009, PR China.
authors.forename: Cuiying
authors.initials: C
authors.lastname: Hu
authors.affiliation: School of Chemistry, Biology, and Material Engineering, Suzhou University of Science and Technology, Suzhou 215009, PR China.
authors.forename: Lishi
authors.initials: L
authors.lastname: Yan
authors.affiliation: School of Chemistry, Biology, and Material Engineering, Suzhou University of Science and Technology, Suzhou 215009, PR China.
authors.forename: Tianyun
authors.initials: T
authors.lastname: Wu
authors.affiliation: School of Chemistry, Biology, and Material Engineering, Suzhou University of Science and Technology, Suzhou 215009, PR China.
authors.forename: Jiaolong
authors.initials: J
authors.lastname: Fu
authors.affiliation: School of Chemistry, Biology, and Material Engineering, Suzhou University of Science and Technology, Suzhou 215009, PR China.
authors.forename: Ming
authors.initials: M
authors.lastname: Qin
mesh_terms: D002480:Cellulase / Q000737:chemistry*; D002482:Cellulose / Q000737:chemistry*; D004121:Dimethyl Sulfoxide / Q000737:chemistry*; D006868:Hydrolysis; D007093:Imidazoles / Q000737:chemistry; D052578:Ionic Liquids / Q000737:chemistry; D010755:Organophosphates / Q000737:chemistry; D056507:Paenibacillus / Q000201:enzymology* / Q000302:isolation & purification; D013696:Temperature
chemical_list: C543070:1-ethyl-3-methyl imidazolium diethyl phosphate; D007093:Imidazoles; D052578:Ionic Liquids; D010755:Organophosphates; D002482:Cellulose; C027433:bagasse; D002480:Cellulase; C109691:microcrystalline cellulose; D004121:Dimethyl Sulfoxide
keywords: Biomass; Cellulase; Dimethyl sulfoxide; Ionic liquid; Pretreatment