title: Stress tolerances of nullmutants of function-unknown genes encoding menadione stress-responsive proteins in Aspergillus nidulans.
abstract: A group of menadione stress-responsive function-unkown genes of Aspergillus nidulans (Locus IDs ANID_03987.1, ANID_06058.1, ANID_10219.1, and ANID_10260.1) was deleted and phenotypically characterized. Importantly, comparative and phylogenetic analyses of the tested A. nidulans genes and their orthologs shed light only on the presence of a TANGO2 domain with NRDE protein motif in the translated ANID_06058.1 gene but did not reveal any recognizable protein-encoding domains in other protein sequences. The gene deletion strains were subjected to oxidative, osmotic, and metal ion stress and, surprisingly, only the ΔANID_10219.1 mutant showed an increased sensitivity to 0.12 mmol l(-1) menadione sodium bisulfite. The gene deletions affected the stress sensitivities (tolerances) irregularly, for example, some strains grew more slowly when exposed to various oxidants and/or osmotic stress generating agents, meanwhile the ΔANID_10260.1 mutant possessed a wild-type tolerance to all stressors tested. Our results are in line with earlier studies demonstrating that the deletions of stress-responsive genes do not confer necessarily any stress-sensitivity phenotypes, which can be attributed to compensatory mechanisms based on other elements of the stress response system with overlapping functions.
authors.affiliation: Faculty of Science and Technology, Department of Biotechnology and Microbiology, University of Debrecen, Debrecen, Hungary.
authors.forename: Éva
authors.initials: É
authors.lastname: Leiter
authors.affiliation: Faculty of Science and Technology, Department of Biotechnology and Microbiology, University of Debrecen, Debrecen, Hungary.
authors.forename: Mihály
authors.initials: M
authors.lastname: Bálint
authors.affiliation: MTA-DE Momentum, Laboratory of Protein Dynamics, Department of Biochemistry and Molecular Biology, University of Debrecen, Debrecen, Hungary.
authors.forename: Márton
authors.initials: M
authors.lastname: Miskei
authors.affiliation: Faculty of Science and Technology, Department of Biotechnology and Microbiology, University of Debrecen, Debrecen, Hungary.
authors.forename: Erzsébet
authors.initials: E
authors.lastname: Orosz
authors.affiliation: Faculty of Science and Technology, Department of Biotechnology and Microbiology, University of Debrecen, Debrecen, Hungary.
authors.forename: Zsuzsa
authors.initials: Z
authors.lastname: Szabó
authors.affiliation: Faculty of Science and Technology, Department of Biotechnology and Microbiology, University of Debrecen, Debrecen, Hungary.
authors.forename: István
authors.initials: I
authors.lastname: Pócsi
mesh_terms: D001233:Aspergillus nidulans / Q000235:genetics* / Q000378:metabolism*; D017353:Gene Deletion; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D006360:Heat-Shock Proteins / Q000235:genetics*; D009997:Osmotic Pressure; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000378:metabolism; D024483:Vitamin K 3 / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D006360:Heat-Shock Proteins; D017382:Reactive Oxygen Species; D024483:Vitamin K 3
keywords: Aspergillus nidulans; Function-unknown genes; Menadione stress; Stress response; Stress tolerance