title: A Supramolecular Gel Approach to Minimize the Neural Cell Damage during Cryopreservation Process.
abstract: The storage method for living cells is one of the major challenges in cell-based applications. Here, a novel supramolecular gel cryopreservation system (BDTC gel system) is introduced, which can observably increase the neural cell viability during cryopreservation process because this system can (1) confine the ice crystal growth in the porous of BDTC gel system, (2) decrease the amount of ice crystallization and cryopreservation system's freezing point, and (3) reduce the change rates of cell volumes and osmotic shock. In addition, thermoreversible BDTC supramolecular gel is easy to be removed after thawing so it does not hinder the adherence, growth, and proliferation of cells. The results of functionality assessments indicate that BDTC gel system can minimize the neural cell damage during cryopreservation process. This method will be potentially applied in cryopreservation of other cell types, tissues, or organs and will benefit cell therapy, tissue engineering, and organs transplantation.
authors.affiliation: School of Materials Science and Engineering, Wuhan University of Technology, Wuhan, Hubei, 430070, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zeng
authors.affiliation: State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, Wuhan, Hubei, 430070, China.
authors.forename: Yixia
authors.initials: Y
authors.lastname: Yin
authors.affiliation: School of Materials Science and Engineering, Wuhan University of Technology, Wuhan, Hubei, 430070, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.affiliation: School of Materials Science and Engineering, Wuhan University of Technology, Wuhan, Hubei, 430070, China.
authors.forename: Wanghui
authors.initials: W
authors.lastname: Hu
authors.affiliation: School of Materials Science and Engineering, Wuhan University of Technology, Wuhan, Hubei, 430070, China.
authors.forename: Chaocan
authors.initials: C
authors.lastname: Zhang
authors.affiliation: School of Materials Science and Engineering, Wuhan University of Technology, Wuhan, Hubei, 430070, China.
authors.forename: Wanyu
authors.initials: W
authors.lastname: Chen
mesh_terms: D000818:Animals; D015925:Cryopreservation / Q000379:methods*; D005782:Gels; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D016716:PC12 Cells; D051381:Rats
chemical_list: D005782:Gels
keywords: cell damage; cryopreservation; gelator; supra­molecular gel