title: The Long Noncoding RNA IFNG-AS1 Promotes T Helper Type 1 Cells Response in Patients with Hashimoto's Thyroiditis.
abstract: The long noncoding (lnc) RNA-Ifng-AS1 plays an essential role in the transcription of the gene encoding IFN-γ by Th1 cells, and its human ortholog, IFNG-AS1, is expressed in human Th1 cells. However, IFNG-AS1 contributing to Th1 cells' response in Hashimoto's thyroiditis (HT) patients has not been reported. Twenty-eight HT patients and 20 healthy controls were enrolled in the study. The proportion of circulating Th1 cells and the level of T-bet, IFNG mRNA were increased in HT patients, the expression of IFNG-AS1 was upregulated and positively correlated with the proportion of circulating Th1 cells or T-bet, and IFNG expression, or serum level of anti-thyroglobulin antibody/thyroperoxidase antibody in HT patients. IFNG-AS1 regulated the expression of IFNG at both transcriptional and translational level in human CD4(+) T cells. Furthermore, strong positive correlations between the increased transcript level of IFNG-AS1 and the increased transcript level of T-bet or IFNG were revealed in thyroid tissues from HT patients. Our results indicate that enhanced expression of lncRNA-IFNG-AS1 contributes to Th1 cell response in HT patients and may be involved in the pathogenesis of HT.
authors.affiliation: Department of Laboratory Medicine, The Affiliated People's Hospital, Jiangsu University, Zhenjiang, 212002, China.|Institute of Laboratory Medicine, Jiangsu Key Laboratory of Laboratory Medicine, Jiangsu University School of Medicine, Zhenjiang, 212013, China.
authors.forename: Huiyong
authors.initials: H
authors.lastname: Peng
authors.affiliation: Department of Endocrinology, The Affiliated People's Hospital, Jiangsu University, Zhenjiang, 212002, China.
authors.forename: Yingzhao
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Institute of Laboratory Medicine, Jiangsu Key Laboratory of Laboratory Medicine, Jiangsu University School of Medicine, Zhenjiang, 212013, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Tian
authors.affiliation: Institute of Laboratory Medicine, Jiangsu Key Laboratory of Laboratory Medicine, Jiangsu University School of Medicine, Zhenjiang, 212013, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Ma
authors.affiliation: Department of Laboratory Medicine, The Affiliated People's Hospital, Jiangsu University, Zhenjiang, 212002, China.
authors.forename: Xinyi
authors.initials: X
authors.lastname: Tang
authors.affiliation: Institute of Laboratory Medicine, Jiangsu Key Laboratory of Laboratory Medicine, Jiangsu University School of Medicine, Zhenjiang, 212013, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Rui
authors.affiliation: Institute of Laboratory Medicine, Jiangsu Key Laboratory of Laboratory Medicine, Jiangsu University School of Medicine, Zhenjiang, 212013, China.
authors.forename: Xinyu
authors.initials: X
authors.lastname: Tian
authors.affiliation: Institute of Laboratory Medicine, Jiangsu Key Laboratory of Laboratory Medicine, Jiangsu University School of Medicine, Zhenjiang, 212013, China.
authors.forename: Chaoming
authors.initials: C
authors.lastname: Mao
authors.affiliation: Department of Pathology, The University of Hong Kong, Hong Kong, China.
authors.forename: Liwei
authors.initials: L
authors.lastname: Lu
authors.affiliation: Institute of Laboratory Medicine, Jiangsu Key Laboratory of Laboratory Medicine, Jiangsu University School of Medicine, Zhenjiang, 212013, China.
authors.forename: Huaxi
authors.initials: H
authors.lastname: Xu
authors.affiliation: Department of General Surgery, The Affiliated People's Hospital, Jiangsu University, Zhenjiang, 212002, China.
authors.forename: Pengcheng
authors.initials: P
authors.lastname: Jiang
authors.affiliation: Department of Laboratory Medicine, The Affiliated People's Hospital, Jiangsu University, Zhenjiang, 212002, China.|Institute of Laboratory Medicine, Jiangsu Key Laboratory of Laboratory Medicine, Jiangsu University School of Medicine, Zhenjiang, 212013, China.
authors.forename: Shengjun
authors.initials: S
authors.lastname: Wang
mesh_terms: D000328:Adult; D000368:Aged; D005260:Female; D005786:Gene Expression Regulation; D050031:Hashimoto Disease / Q000097:blood / Q000235:genetics* / Q000473:pathology; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis* / Q000235:genetics; D008297:Male; D008875:Middle Aged; D062085:RNA, Long Noncoding / Q000097:blood* / Q000235:genetics; D018417:Th1 Cells / Q000378:metabolism / Q000473:pathology; D013954:Thyroglobulin / Q000097:blood; D013961:Thyroid Gland / Q000378:metabolism / Q000473:pathology; D014158:Transcription, Genetic
chemical_list: C573415:IFNG protein, human; D062085:RNA, Long Noncoding; D007371:Interferon-gamma; D013954:Thyroglobulin