title: Systemic Gene Therapy for Targeting the CNS.
abstract: Systemic gene delivery is useful for modeling and treatment of a body-wide disease. Recently, it has been shown that certain agents, when delivered systemically, can efficiently target the central nervous system. This technique has been used to model and treat rodent models of neurological disease with unprecedented success. Here, we describe intravenous delivery in neonate and adult mice. These techniques are easily learned and have minimal equipment requirements.
authors.affiliation: Department of Translational Science& Molecular Medicine, Michigan State University, Grand Rapids, MI, USA.|Neuroscience Graduate Program, University of Cincinnati, 460 W 12th Ave, BRT 698, Cincinnati, OH, US.
authors.forename: Sara E
authors.initials: SE
authors.lastname: Gombash
authors.affiliation: Department of Neuroscience, Ohio State University, 460 W 12th Ave, BRT 698, Columbus, OH, 43210, USA. Kevin.Foust@osumc.edu.
authors.forename: Kevin D
authors.initials: KD
authors.lastname: Foust
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002490:Central Nervous System / Q000378:metabolism*; D000229:Dependovirus / Q000235:genetics*; D004195:Disease Models, Animal; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors / Q000008:administration & dosage; D007275:Injections, Intravenous; D051379:Mice; D009422:Nervous System Diseases / Q000378:metabolism / Q000628:therapy*; D009928:Organ Specificity; D016896:Treatment Outcome
keywords: Adeno-associated virus type 9; Intravenous injection; Superficial temporal vein; Systemic gene therapy; Tail vein