title: Study of Cytokine Profile of Cultured "Early" and "Late" Endothelial Progenitor Cells in Peripheral Blood of Chronic Heart Failure Patients after Mobilization Course with Granulocyte Colony-Stimulating Factor.
abstract: The effect of cell culturing protocol with various adhesion proteins and different culture time on the profile of cytokine and growth factors produced by endothelial progenitor cells harvested after mobilization with granulocyte colony-stimulating factor was examined in patients with chronic heart failure. The endothelial progenitor cells cultured on fibronectin or gelatin produced a broad and overall similar spectrum of cytokines and growth factors, the levels of which depended on the culture time. On culture day 16, the cells grown on fibronectin diminished the production of cytokines and growth factors (IL-10, IL-18, IL-8, erythropoietin, and VEGF), while the cells grown on gelatin down-regulated the synthesis of TNF-α, IL-8, and erythropoietin, although they up-regulated the production of IL-10, IL-18, and VEGF.
authors.affiliation: Research Institute of Experimental and Clinical Lymphology, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia. bond802888@yandex.ru.|E. N. Meshalkin Novosibirsk Research Institute of Circulation Pathology, Ministry of Health of the Russian Federation, Novosibirsk, Russia. bond802888@yandex.ru.
authors.forename: N A
authors.initials: NA
authors.lastname: Bondarenko
authors.affiliation: Research Institute of Experimental and Clinical Lymphology, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia.|E. N. Meshalkin Novosibirsk Research Institute of Circulation Pathology, Ministry of Health of the Russian Federation, Novosibirsk, Russia.
authors.forename: O V
authors.initials: OV
authors.lastname: Poveshchenko
authors.affiliation: Research Institute of Experimental and Clinical Lymphology, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia.|E. N. Meshalkin Novosibirsk Research Institute of Circulation Pathology, Ministry of Health of the Russian Federation, Novosibirsk, Russia.
authors.forename: A P
authors.initials: AP
authors.lastname: Lykov
authors.affiliation: Research Institute of Experimental and Clinical Lymphology, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia.|E. N. Meshalkin Novosibirsk Research Institute of Circulation Pathology, Ministry of Health of the Russian Federation, Novosibirsk, Russia.
authors.forename: I I
authors.initials: II
authors.lastname: Kim
authors.affiliation: Research Institute of Experimental and Clinical Lymphology, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia.
authors.forename: M A
authors.initials: MA
authors.lastname: Surovtseva
authors.affiliation: Research Institute of Experimental and Clinical Lymphology, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia.|E. N. Meshalkin Novosibirsk Research Institute of Circulation Pathology, Ministry of Health of the Russian Federation, Novosibirsk, Russia.
authors.forename: A F
authors.initials: AF
authors.lastname: Poveshchenko
authors.affiliation: E. N. Meshalkin Novosibirsk Research Institute of Circulation Pathology, Ministry of Health of the Russian Federation, Novosibirsk, Russia.
authors.forename: E A
authors.initials: EA
authors.lastname: Pokushalov
authors.affiliation: E. N. Meshalkin Novosibirsk Research Institute of Circulation Pathology, Ministry of Health of the Russian Federation, Novosibirsk, Russia.
authors.forename: A B
authors.initials: AB
authors.lastname: Romanov
authors.affiliation: E. N. Meshalkin Novosibirsk Research Institute of Circulation Pathology, Ministry of Health of the Russian Federation, Novosibirsk, Russia.
authors.forename: A M
authors.initials: AM
authors.lastname: Karas'kov
authors.affiliation: Research Institute of Experimental and Clinical Lymphology, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia.|E. N. Meshalkin Novosibirsk Research Institute of Circulation Pathology, Ministry of Health of the Russian Federation, Novosibirsk, Russia.
authors.forename: V I
authors.initials: VI
authors.lastname: Konenkov
mesh_terms: D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism*; D066026:Endothelial Progenitor Cells / Q000187:drug effects* / Q000378:metabolism*; D004921:Erythropoietin / Q000378:metabolism; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D006333:Heart Failure / Q000378:metabolism*; D006801:Humans; D016753:Interleukin-10 / Q000378:metabolism; D020382:Interleukin-18 / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D016207:Cytokines; C508609:IL10 protein, human; D020382:Interleukin-18; D016209:Interleukin-8; D042461:Vascular Endothelial Growth Factor A; D004921:Erythropoietin; D016753:Interleukin-10; D016179:Granulocyte Colony-Stimulating Factor
keywords: chronic heart failure; cytokines; endothelial progenitor cells; growth factors