title: [Isolation of enteropathogenic Escherichia coli O157:H16 identified in a diarrhea case in a child and his household contacts in La Pampa Province, Argentina].
abstract: Enteropathogenic Escherichia coli (EPEC) is a major causative agent of acute diarrhea in children in developing countries. This pathotype is divided into typical EPEC (tEPEC) and atypical EPEC (aEPEC), based on the presence of the bfp virulence factor associated with adhesion, encoded in the pEAF plasmid. In the present study, the isolation of aEPEC O157:H16 from a bloody diarrhea case in a child and his household contacts (mother, father and sister) is described. The strain was characterized as E. coli O157:H16 eae-ɛ-positive, sorbitol fermenter with β-glucuronidase activity, susceptible to all antimicrobials tested, and negative for virulence factors stx1, stx2, ehxA and bfp. XbaI-PFGE performed on all isolates showed the AREXHX01.1040 macrorestriction pattern, with 100% similarity. These results highlight the importance of epidemiological surveillance of E. coli O157-associated diarrhea cases identified in children and their family contacts, as well as the incorporation of molecular techniques that allow the detection of the different E. coli pathotypes.
authors.affiliation: Laboratorio de Microbiología, Hospital Gobernador Centeno, General Pico, La Pampa, Argentina; Comité de Epidemiología, Hospital Gobernador Centeno, General Pico, La Pampa, Argentina. Electronic address: ivanasilveyra@hotmail.com.
authors.forename: Ivana M
authors.initials: IM
authors.lastname: Silveyra
authors.affiliation: Laboratorio de Microbiología, Hospital Gobernador Centeno, General Pico, La Pampa, Argentina; Comité de Epidemiología, Hospital Gobernador Centeno, General Pico, La Pampa, Argentina.
authors.forename: Adriana M
authors.initials: AM
authors.lastname: Pereyra
authors.affiliation: Comité de Epidemiología, Hospital Gobernador Centeno, General Pico, La Pampa, Argentina.
authors.forename: María G
authors.initials: MG
authors.lastname: Alvarez
authors.affiliation: Comité de Epidemiología, Hospital Gobernador Centeno, General Pico, La Pampa, Argentina.
authors.forename: Mariana D
authors.initials: MD
authors.lastname: Villagran
authors.affiliation: Laboratorio de Microbiología, Hospital Gobernador Centeno, General Pico, La Pampa, Argentina.
authors.forename: Andrea B
authors.initials: AB
authors.lastname: Baroni
authors.affiliation: Servicio Fisiopatogenia, Instituto Nacional de Enfermedades Infecciosas-ANLIS «Dr. Carlos G. Malbrán», Buenos Aires, Argentina.
authors.forename: Natalia
authors.initials: N
authors.lastname: Deza
authors.affiliation: Servicio Fisiopatogenia, Instituto Nacional de Enfermedades Infecciosas-ANLIS «Dr. Carlos G. Malbrán», Buenos Aires, Argentina.
authors.forename: Claudia C
authors.initials: CC
authors.lastname: Carbonari
authors.affiliation: Servicio Fisiopatogenia, Instituto Nacional de Enfermedades Infecciosas-ANLIS «Dr. Carlos G. Malbrán», Buenos Aires, Argentina.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Miliwebsky
authors.affiliation: Servicio Fisiopatogenia, Instituto Nacional de Enfermedades Infecciosas-ANLIS «Dr. Carlos G. Malbrán», Buenos Aires, Argentina.
authors.forename: Marta
authors.initials: M
authors.lastname: Rivas
mesh_terms: D001118:Argentina; D002675:Child, Preschool; D003967:Diarrhea / Q000382:microbiology*; D054308:Enteropathogenic Escherichia coli / Q000302:isolation & purification*; D019453:Escherichia coli O157 / Q000302:isolation & purification*; D005192:Family Health; D005260:Female; D006801:Humans; D008297:Male
keywords: Diarrea; Diarrhea; EPEC; Escherichia coli O157:H16