title: [Comparative study of two techniques of ciclosporine monitoring].
abstract: Ciclosporine (CsA) is an immunosuppressant drug used in bone marrow transplantation in order to extend allograft survival. Despite its efficiency, CsA can expose to therapeutic failure or to toxicity because of underdosing or overdosage. So, many techniques of monitoring CsA in blood were used, the referance one is the chromatographic technique then, the automated techniques: fluorescence polarization immunoassay (FPIA) and chimiluminescent microparticle immunoassay (CMIA). In this study, we aimed to compare the results of CsA concentrations measured by the two automised techniques. Statistical studies showed that the two techniques were repeatable and reproductible. Results obtained by FPIA were slightly higher than those obtained by CMIA but without a significative difference. In conclusion, FPIA technique could be used to measure CsA blood concentration in replacement of CMIA in case of technical problems.
authors.affiliation: Service de pharmacologie clinique, Centre national de pharmacovigilance, Tunis, Tunisie, Faculté de médecine, Université de Tunis El Manar, Tunis, Tunisie.
authors.forename: Rim
authors.initials: R
authors.lastname: Charfi
authors.affiliation: Service de pharmacologie clinique, Centre national de pharmacovigilance, Tunis, Tunisie.
authors.forename: Hanène
authors.initials: H
authors.lastname: El Jebari
authors.affiliation: Service de pharmacologie clinique, Centre national de pharmacovigilance, Tunis, Tunisie, Faculté de médecine, Université de Tunis El Manar, Tunis, Tunisie.
authors.forename: Emna
authors.initials: E
authors.lastname: Gaïes
authors.affiliation: Service de pharmacologie clinique, Centre national de pharmacovigilance, Tunis, Tunisie, Faculté de médecine, Université de Tunis El Manar, Tunis, Tunisie.
authors.forename: Ons
authors.initials: O
authors.lastname: Charfi
authors.affiliation: Service de pharmacologie clinique, Centre national de pharmacovigilance, Tunis, Tunisie.
authors.forename: Nadia
authors.initials: N
authors.lastname: Jebabli
authors.affiliation: Service de pharmacologie clinique, Centre national de pharmacovigilance, Tunis, Tunisie, Institut supérieur de biotechnologie de Sidi Thabet Biotechnopôle, Université de la Manouba, Tunis, Tunisie.
authors.forename: Riahi
authors.initials: R
authors.lastname: Thouraya
authors.affiliation: Institut supérieur de biotechnologie de Sidi Thabet Biotechnopôle, Université de la Manouba, Tunis, Tunisie.
authors.forename: Mhamed
authors.initials: M
authors.lastname: Ben Messaouda
authors.affiliation: Service de pharmacologie clinique, Centre national de pharmacovigilance, Tunis, Tunisie, Faculté de médecine, Université de Tunis El Manar, Tunis, Tunisie.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Lakhal
authors.affiliation: Service de pharmacologie clinique, Centre national de pharmacovigilance, Tunis, Tunisie, Faculté de médecine, Université de Tunis El Manar, Tunis, Tunisie.
authors.forename: Anis
authors.initials: A
authors.lastname: Klouz
authors.affiliation: Service de pharmacologie clinique, Centre national de pharmacovigilance, Tunis, Tunisie, Faculté de médecine, Université de Tunis El Manar, Tunis, Tunisie.
authors.forename: Issam
authors.initials: I
authors.lastname: Salouage
authors.affiliation: Service de pharmacologie clinique, Centre national de pharmacovigilance, Tunis, Tunisie, Faculté de médecine, Université de Tunis El Manar, Tunis, Tunisie.
authors.forename: Sameh
authors.initials: S
authors.lastname: Trabelsi
mesh_terms: D001774:Blood Chemical Analysis / Q000295:instrumentation / Q000379:methods / Q000592:standards; D016572:Cyclosporine / Q000627:therapeutic use*; D016903:Drug Monitoring / Q000295:instrumentation / Q000379:methods* / Q000592:standards; D016231:Fluorescence Polarization Immunoassay / Q000295:instrumentation / Q000379:methods / Q000592:standards; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods / Q000592:standards; D007166:Immunosuppressive Agents / Q000627:therapeutic use*; D008163:Luminescent Measurements / Q000295:instrumentation / Q000379:methods* / Q000592:standards; D060735:Pharmacovigilance; D011786:Quality Control; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D007166:Immunosuppressive Agents; D016572:Cyclosporine
keywords: bone marrow allograft; ciclosporine; immunofluorescence polarization; microparticular immunochimiluminescence