title: Characterisation of the large-scale production process of oyster mushroom (Pleurotus ostreatus) with the analysis of succession and spatial heterogeneity of lignocellulolytic enzyme activities.
abstract: Oyster mushroom (Pleurotus ostreatus) lignocellulolytic enzyme activity pattern and variation was investigated in a large-scale facility from spawning until the end of the second flush. In the first cultivation cycle laccase production reached its peak during vegetative growth stage, while manganese-peroxidase showed the highest activity during fruiting body induction. Cellulose and hemicellulose degrading enzymes had maximal activity at the beginning of flush and harvest stage. The enzyme activities showed similar tendencies among five different mushroom substrate blocks representing a production house. The spatial variability analysis of enzyme activities pointed out the within substrate block heterogeneity as the main source if variation. This result was confirmed by Combined Cluster and Discriminant Analysis (CCDA) method showing minimal among block heterogeneity considering the whole investigation period; furthermore in the first cultivation cycle all blocks were grouped into one cluster.
authors.affiliation: Department of Microbiology, Eötvös Loránd University, Pázmány Péter sétány 1/C, 1117 Budapest, Hungary. Electronic address: banfir@gmail.com.
authors.forename: Renáta
authors.initials: R
authors.lastname: Bánfi
authors.affiliation: Department of Microbiology, Eötvös Loránd University, Pázmány Péter sétány 1/C, 1117 Budapest, Hungary.
authors.forename: Zsuzsanna
authors.initials: Z
authors.lastname: Pohner
authors.affiliation: Department of Physical and Applied Geology, Eötvös Loránd University, Pázmány Péter sétány 1/C, 1117 Budapest, Hungary.
authors.forename: József
authors.initials: J
authors.lastname: Kovács
authors.affiliation: Department of Microbiology, Eötvös Loránd University, Pázmány Péter sétány 1/C, 1117 Budapest, Hungary.
authors.forename: Szabina
authors.initials: S
authors.lastname: Luzics
authors.affiliation: Pilze-Nagy Ltd., Talfája 50, H-6000 Kecskemét, Hungary.
authors.forename: Adrienn
authors.initials: A
authors.lastname: Nagy
authors.affiliation: Pilze-Nagy Ltd., Talfája 50, H-6000 Kecskemét, Hungary.
authors.forename: Melinda
authors.initials: M
authors.lastname: Dudás
authors.affiliation: Department of Physical and Applied Geology, Eötvös Loránd University, Pázmány Péter sétány 1/C, 1117 Budapest, Hungary.
authors.forename: Péter
authors.initials: P
authors.lastname: Tanos
authors.affiliation: Department of Microbiology, Eötvös Loránd University, Pázmány Péter sétány 1/C, 1117 Budapest, Hungary.
authors.forename: Károly
authors.initials: K
authors.lastname: Márialigeti
authors.affiliation: Department of Microbiology, Eötvös Loránd University, Pázmány Péter sétány 1/C, 1117 Budapest, Hungary.
authors.forename: Balázs
authors.initials: B
authors.lastname: Vajna
mesh_terms: D002482:Cellulose / Q000378:metabolism; D003470:Culture Media / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D042845:Laccase / Q000235:genetics / Q000378:metabolism*; D008031:Lignin / Q000378:metabolism; D020076:Pleurotus / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development
chemical_list: D003470:Culture Media; D005656:Fungal Proteins; D002482:Cellulose; D008031:Lignin; D042845:Laccase
keywords: Analysis of variability; CCDA; Large-scale mushroom production; Lignocellulolytic enzyme activity; Pleurotus ostreatus