title: Platforms Formed from a Three-Dimensional Cu-Based Zwitterionic Metal-Organic Framework and Probe ss-DNA: Selective Fluorescent Biosensors for Human Immunodeficiency Virus 1 ds-DNA and Sudan Virus RNA Sequences.
abstract: We herein report a water-stable three-dimensional Cu-based metal-organic framework (MOF) 1 supported by a tritopic quaternized carboxylate and 4,4'-dipyridyl sulfide as an ancillary ligand. This MOF exhibits unique pore shapes with aromatic rings, positively charged pyridinium and unsaturated Cu(II) cation centers, free carboxylates, tessellating H2O, and coordinating SO4(2-) on the pore surface. Compound 1 can interact with two carboxyfluorescein (FAM)-labeled single-stranded DNA sequences (probe ss-DNA, delineated as P-DNA) through electrostatic, π-stacking, and/or hydrogen-bonding interactions to form two P-DNA@1 systems, and thus quench the fluorescence of FAM via a photoinduced electron-transfer process. These P-DNA@1 systems can be used as effective fluorescent sensors for human immunodeficiency virus 1 double-stranded DNA and Sudan virus RNA sequences, respectively, with detection limits of 196 and 73 pM, respectively.
authors.affiliation: Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University , Guangzhou 510515, P. R. China.
authors.forename: Shui-Ping
authors.initials: SP
authors.lastname: Yang
authors.affiliation: Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University , Guangzhou 510515, P. R. China.
authors.forename: Shao-Rui
authors.initials: SR
authors.lastname: Chen
authors.affiliation: Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University , Guangzhou 510515, P. R. China.
authors.forename: Shu-Wen
authors.initials: SW
authors.lastname: Liu
authors.affiliation: Department of Chemistry and Materials Engineering, Jiangsu Key Laboratory of Advanced Functional Materials, Changshu Institute of Technology , Changshu 215500, Jiangsu P. R. China.
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Tang
authors.affiliation: Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University , Guangzhou 510515, P. R. China.
authors.forename: Liang
authors.initials: L
authors.lastname: Qin
authors.affiliation: Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University , Guangzhou 510515, P. R. China.
authors.forename: Gui-Hua
authors.initials: GH
authors.lastname: Qiu
authors.affiliation: Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University , Guangzhou 510515, P. R. China.
authors.forename: Jin-Xiang
authors.initials: JX
authors.lastname: Chen
authors.affiliation: Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University , Guangzhou 510515, P. R. China.
authors.forename: Wen-Hua
authors.initials: WH
authors.lastname: Chen
mesh_terms: D015374:Biosensing Techniques; D003300:Copper / Q000737:chemistry*; D018360:Crystallography, X-Ray; D004277:DNA, Single-Stranded / Q000032:analysis*; D004279:DNA, Viral / Q000032:analysis*; D029043:Ebolavirus / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D015497:HIV-1 / Q000737:chemistry; D008958:Models, Molecular; D015394:Molecular Structure; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D012367:RNA, Viral / Q000032:analysis*
chemical_list: D004277:DNA, Single-Stranded; D004279:DNA, Viral; D005456:Fluorescent Dyes; D009942:Organometallic Compounds; D012367:RNA, Viral; D003300:Copper