title: Gga-miR-101-3p Plays a Key Role in Mycoplasma gallisepticum (HS Strain) Infection of Chicken.
abstract: Mycoplasma gallisepticum (MG), one of the most pathogenic Mycoplasma, has caused tremendous economic loss in the poultry industry. Recently, increasing evidence has suggested that micro ribonucleic acids (miRNAs) are involved in microbial pathogenesis. However, little is known about potential roles of miRNAs in MG infection of chicken. In the present study, using miRNA Solexa sequencing we have found that gga-miR-101-3p was up-regulated in the lungs of MG-infected chicken embryos. Moreover, gga-miR-101-3p regulated expression of the host enhancer of zeste homolog 2 (EZH2) through binding to the 3' un-translated region (3'-UTR) of EZH2 gene. Over-expression of gga-miR-101-3p significantly inhibited EZH2 expression and hence inhibited proliferation of chicken embryonic fibroblast (DF-1 cells) by blocking the G1-to-S phase transition. Similar results were obtained in MG-infected chicken embryos and DF-1 cells, where gga-miR-101-3p was significantly up-regulated, while EZH2 was significantly down-regulated. This study reveals that gga-miR-101-3p plays an important role in MG infection through regulation of EZH2 expression and provides a new insight into the mechanisms of MG pathogenesis.
authors.affiliation: Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China. chenjiao123@webmail.hzau.edu.cn.
authors.forename: Jiao
authors.initials: J
authors.lastname: Chen
authors.affiliation: Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China. wangzaiwei@webmail.hzau.edu.cn.
authors.forename: Zaiwei
authors.initials: Z
authors.lastname: Wang
authors.affiliation: China National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China. bidingren@mail.hzau.edu.cn.
authors.forename: Dingren
authors.initials: D
authors.lastname: Bi
authors.affiliation: Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China. houyue@webmail.hzau.edu.cn.
authors.forename: Yue
authors.initials: Y
authors.lastname: Hou
authors.affiliation: Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China. zyb@webmail.hzau.edu.cn.
authors.forename: Yabo
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Department of Biological Sciences and Border Biomedical Research Center, University of Texas at El Paso, El Paso, TX 79968, USA. jsun@utep.edu.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Sun
authors.affiliation: Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China. xlpengsishun@mail.hzau.edu.cn.|China National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China. xlpengsishun@mail.hzau.edu.cn.
authors.forename: Xiuli
authors.initials: X
authors.lastname: Peng
mesh_terms: D020413:3' Untranslated Regions; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002453:Cell Cycle; D049109:Cell Proliferation; D002642:Chick Embryo; D002645:Chickens; D005786:Gene Expression Regulation; D035683:MicroRNAs / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D009175:Mycoplasma Infections / Q000662:veterinary*; D045686:Mycoplasma gallisepticum; D009690:Nucleic Acid Conformation; D011201:Poultry Diseases / Q000235:genetics* / Q000382:microbiology*; D034622:RNA Interference; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics; D016415:Sequence Alignment
chemical_list: D020413:3' Untranslated Regions; D035683:MicroRNAs; D012333:RNA, Messenger
keywords: EZH2; Mycoplasma gallisepticum (HS strain); chicken; gga-miR-101-3p