title: Direct Fluorescent Detection of a Polymethoxyflavone in Cell Culture and Mouse Tissue.
abstract: Convenient detection methods for bioactive food compounds and their metabolites in biological samples are needed to better understand their mechanism of actions. Herein, we developed a novel approach to directly monitor and visualize the distribution of 5,3',4'-tridemethylnobiletin (TDN), a unique polymethoxyflavone metabolite derived from citrus polymethoxyflavone, in biological samples such as cultured cells and mouse colonic tissues. Our results showed that a fluorescent conjugate could be formed between TDN and 2-aminoethyl diphenyl borate (DPBA) under simple reaction conditions, which was confirmed by both Raman spectroscopy and mass spectroscopy. We further demonstrated the application of DPBA-based conjugation reaction in the characterization of TDN in different biological samples including floating cells, adherent cells, and animal tissues. This is the first report demonstrating direct fluorescent detection of polymethoxyflavone in biological samples.
authors.affiliation: Department of Food Science, University of Massachusetts , Amherst, Massachusetts 01003, United States.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Food Science, University of Massachusetts , Amherst, Massachusetts 01003, United States.
authors.forename: Mingyue
authors.initials: M
authors.lastname: Song
authors.affiliation: Department of Food Science, University of Massachusetts , Amherst, Massachusetts 01003, United States.
authors.forename: Xian
authors.initials: X
authors.lastname: Wu
authors.affiliation: Department of Food Science, University of Massachusetts , Amherst, Massachusetts 01003, United States.|Institute of Agro-Products Processing Science and Technology, Chinese Academy of Agricultural Sciences , Beijing 100193, People's Republic of China.
authors.forename: Jinkai
authors.initials: J
authors.lastname: Zheng
authors.affiliation: Department of Food Science, University of Massachusetts , Amherst, Massachusetts 01003, United States.
authors.forename: Lili
authors.initials: L
authors.lastname: He
authors.affiliation: Department of Food Science, University of Massachusetts , Amherst, Massachusetts 01003, United States.
authors.forename: David Julian
authors.initials: DJ
authors.lastname: McClements
authors.affiliation: Department of Food Science, University of Massachusetts , Amherst, Massachusetts 01003, United States.
authors.forename: Eric
authors.initials: E
authors.lastname: Decker
authors.affiliation: Department of Food Science, University of Massachusetts , Amherst, Massachusetts 01003, United States.|College of Bioscience and Biotechnology, Hunan Agricultural University , Changsha, Hunan 410128, People's Republic of China.
authors.forename: Hang
authors.initials: H
authors.lastname: Xiao
mesh_terms: D000818:Animals; D001896:Boron Compounds / Q000737:chemistry; D018938:Caco-2 Cells; D002957:Citrus / Q000737:chemistry; D003106:Colon / Q000737:chemistry; D047309:Flavones / Q000032:analysis*; D005419:Flavonoids / Q000032:analysis* / Q000737:chemistry; D005456:Fluorescent Dyes; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D051379:Mice; D009281:Naphthalenes; D013050:Spectrometry, Fluorescence; D013059:Spectrum Analysis, Raman
chemical_list: C538758:2-aminoethyl diphenylborinate; C000609644:5,3',4'-tridemethylnobiletin; D001896:Boron Compounds; D047309:Flavones; D005419:Flavonoids; D005456:Fluorescent Dyes; D009281:Naphthalenes; C573721:1,1,6-trimethyl-1,2-dihydronaphthalene
keywords: colon cancer cell; fluorescence detection; imaging; mouse tissue; polymethoxyflavone