title: [GLUCOSE METABOLISM IN SURFACTANTS PRODUCER NOCARDIA VACCINII IMV B-7405].
abstract: Key enzymes of glucose metabolism were detected in the cells of surfactants producer Nocardia vaccinii IMV B-7405 grown on this substrate. It has been established that glucose catabolism is performed through gluconate (FAD(+)-dependent glucose dehydrogenase activity 698 ± 35 nmol x min(-1) x mg(-1) of protein). Oxidation of gluconate to 6-phosphogluconate is catalised by gluconokinase (178 ± 9 nmol x min(-1) x mg(-1) of protein). 6-Phosphogluconate was involved into pentose phosphate cycle by constitutive NADP(+)-dependent 6-phosphogluconate dehydrogenase (activity 357 ± 17 nmol x min(-1) x mg(-1) of protein). The data obtained serve as the basis for theoretical calculations of optimal molar ratio of concentrations of energetically nonequivalent substrates for intensifying the surfactants synthesis on their mixture.
authors.forename: T P
authors.initials: TP
authors.lastname: Pirog
authors.forename: T A
authors.initials: TA
authors.lastname: Shevchuk
authors.forename: K A
authors.initials: KA
authors.lastname: Beregova
mesh_terms: D050260:Carbohydrate Metabolism; D005942:Gluconates / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D018086:Glutamate Dehydrogenase (NADP+) / Q000378:metabolism; D005993:Glycerolphosphate Dehydrogenase / Q000378:metabolism*; D009615:Nocardia / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism*; D010427:Pentose Phosphate Pathway / Q000502:physiology; D010734:Phosphogluconate Dehydrogenase / Q000378:metabolism; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000378:metabolism; D013379:Substrate Specificity
chemical_list: D005942:Gluconates; D005993:Glycerolphosphate Dehydrogenase; D010734:Phosphogluconate Dehydrogenase; C098831:glycerol-3-phosphate oxidase; D018086:Glutamate Dehydrogenase (NADP+); D017853:Phosphotransferases (Alcohol Group Acceptor); C020578:gluconokinase; D005947:Glucose; C008884:6-phosphogluconic acid