title: Anther Culture in Pepper (Capsicum annuum L.).
abstract: Anther culture is the most popular of the techniques used to induce microspore embryogenesis. This technique is well set up in a wide range of crops, including pepper. In this chapter, a protocol for anther culture in pepper is described. The protocol presented hereby includes the steps from the selection of buds from donor plants to the regeneration and acclimatization of doubled haploid plants derived from the embryos, as well as a description of how to analyze the ploidy level of the regenerated plants.
authors.affiliation: COMAV - Universitat Politècnica de València. CPI, Edificio 8E - Escalera I, Camino de Vera, s/n, 46022, Valencia, Spain.
authors.forename: Verónica
authors.initials: V
authors.lastname: Parra-Vega
authors.affiliation: COMAV - Universitat Politècnica de València. CPI, Edificio 8E - Escalera I, Camino de Vera, s/n, 46022, Valencia, Spain. seguisim@btc.upv.es.
authors.forename: Jose M
authors.initials: JM
authors.lastname: Seguí-Simarro
mesh_terms: D002212:Capsicum / Q000235:genetics / Q000254:growth & development*; D035264:Flowers / Q000235:genetics / Q000254:growth & development; D018525:Germination / Q000235:genetics; D006238:Haploidy; D063245:Plant Development / Q000235:genetics*; D058439:Plant Somatic Embryogenesis Techniques / Q000379:methods*; D011058:Pollen / Q000235:genetics / Q000254:growth & development; D012038:Regeneration / Q000235:genetics; D046509:Tissue Culture Techniques / Q000379:methods*
keywords: Androgenesis; Doubled haploid; Embryogenesis; Haploid; Microspores; Pollen; Tissue culture