title: Antithrombotic Effects of Amaranthus hypochondriacus Proteins in Rats.
abstract: Cardiovascular disease (CVD) is a major cause of disability and premature death throughout the world. Diets with antithrombotic components offer a convenient and effective way of preventing and reducing CVD incidence. The aim of the present work was to assess in vivo and ex vivo effects of Amaranthus hypochondriacus proteins on platelet plug formation and coagulation cascade. Amaranth proteins were orally administrated to rats (AG, 8 animals) and bleeding time was determined showing no significant difference compared with control rats (CG, 7 animals). However, results show a strong tendency, suggesting that amaranth proteins are involved in the inhibition of thrombus formation. Non-anticoagulated blood extracted from animals was analyzed with the hemostatometer, where AG parameters obtained were twice the values showed by CG. The clotting tests, thrombin time (TT) and activated partial thromboplastin time (APTT), presented a 17 and 14% clotting formation increase respectively when comparing AG with CG. The ex-vivo assays confirm the hypothesis inferring that amaranth proteins are a potential antithrombotic agent.
authors.affiliation: Centro de Investigación y Desarrollo en Criotecnología de Alimentos (CIDCA), Calle 47 y 116, 1900, La Plata, Argentina.|Facultad de Ciencias Exactas, Universidad Nacional de La Plata. CCT, La Plata, CONICET (Consejo Nacional de Investigaciones Científicas y Técnicas), La Plata, Argentina.
authors.forename: Ana Clara
authors.initials: AC
authors.lastname: Sabbione
authors.affiliation: Centro de Investigaciones Cardiovasculares (CIC), Facultad de Ciencias Médicas, Universidad Nacional de La Plata, Avenida 60 y 120 2° piso, La Plata, Argentina.
authors.forename: Gustavo
authors.initials: G
authors.lastname: Rinaldi
authors.affiliation: Centro de Investigación y Desarrollo en Criotecnología de Alimentos (CIDCA), Calle 47 y 116, 1900, La Plata, Argentina.|Facultad de Ciencias Exactas, Universidad Nacional de La Plata. CCT, La Plata, CONICET (Consejo Nacional de Investigaciones Científicas y Técnicas), La Plata, Argentina.
authors.forename: María Cristina
authors.initials: MC
authors.lastname: Añón
authors.affiliation: Centro de Investigación y Desarrollo en Criotecnología de Alimentos (CIDCA), Calle 47 y 116, 1900, La Plata, Argentina. aascilingo@gmail.com.|Facultad de Ciencias Exactas, Universidad Nacional de La Plata. CCT, La Plata, CONICET (Consejo Nacional de Investigaciones Científicas y Técnicas), La Plata, Argentina. aascilingo@gmail.com.
authors.forename: Adriana A
authors.initials: AA
authors.lastname: Scilingo
mesh_terms: D027721:Amaranthus / Q000737:chemistry*; D000818:Animals; D001777:Blood Coagulation / Q000187:drug effects; D004195:Disease Models, Animal; D005343:Fibrinolytic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D055951:Functional Food; D008297:Male; D010314:Partial Thromboplastin Time; D008517:Phytotherapy; D010940:Plant Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D011208:Powders; D051381:Rats; D017208:Rats, Wistar; D012639:Seeds / Q000737:chemistry; D013927:Thrombosis / Q000097:blood / Q000188:drug therapy*
chemical_list: D005343:Fibrinolytic Agents; D010940:Plant Proteins; D011208:Powders
keywords: Amaranth proteins; Animals assays; Antithrombotic activity; Functional food