title: Dual-Targeting Nanovesicles for In Situ Intracellular Imaging of and Discrimination between Wild-type and Mutant p53.
abstract: p53 is a tumor-suppressor protein related to the cell cycle and programmed cell apoptosis. Herein, dual-targeting nanovesicles are designed for in situ imaging of intracellular wild-type p53 (WTp53) and mutant p53 (MUp53). Nanovesicle-encapsulated plasmonic gold nanoparticles (AuNPs) were functionalized with consensus DNA duplexes, and a fluorescein isothiocyanate (FITC)-marked anti-MUp53 antibody was conjugated to the nanovesicle surface. After entering the cytoplasm, the released AuNPs aggregated through recognition of WTp53 and the double-stranded DNA. The color changes of AuNPs were observed using dark-field microscopy, which showed the intracellular WTp53 distribution. The MUp53 location was detected though the immunological recognition between FITC-labeled anti-MUp53 and MUp53. Thus, a one-step incubation method for the in situ imaging of intracellular WTp53 and MUp53 was obtained; this was used to monitor the p53 level under a drug treatment.
authors.affiliation: Key Laboratory for Advanced Materials, East China University of Science and Technology, Shanghai, 200237 (P.R. China).
authors.forename: Ruocan
authors.initials: R
authors.lastname: Qian
authors.affiliation: Key Laboratory for Advanced Materials, East China University of Science and Technology, Shanghai, 200237 (P.R. China).
authors.forename: Yue
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Key Laboratory for Advanced Materials, East China University of Science and Technology, Shanghai, 200237 (P.R. China). ytlong@ecust.edu.cn.
authors.forename: Yi-Tao
authors.initials: YT
authors.lastname: Long
mesh_terms: D006367:HeLa Cells; D006801:Humans; D057054:Molecular Imaging; D009154:Mutation; D049329:Nanostructures; D016159:Tumor Suppressor Protein p53 / Q000737:chemistry* / Q000235:genetics
chemical_list: D016159:Tumor Suppressor Protein p53
keywords: dual targeting; fluorescence imaging; nanovesicles; p53; plasmonic imaging