title: Post-conditioning through lower limb ischemia-reperfusion can alleviate lung ischemia-reperfusion injury.
abstract: OBJECTIVE
  Operation on the infrarenal aorta could cause ischemic-reperfusion (IR) injury in local tissues and remote organs (e.g. the lung). We aim to explore the method of reducing lung ischemia-reperfusion damage after lower limb IR with post conditioning (LIPC).
  
  
  METHODS
  Bilateral lower limb ischemia was performed in Sprague-Dawley (SD) rats, and then animals were divided into 4 groups: IR-Sham-operated, IR, post conditioned-IR (LIPC) and bilateral lower limb ischemia (LIR). The serum free radical, histological changes, Wet/Dry (W/D) ratio, levels of TNF-α, IL-6, cytokines and chemokines were tested and compared.
  
  
  RESULTS
  Post-conditioning could ameliorate histological injuries in the lung when compared to IR group. The serum free radical is significantly lower in LIPC group than IR groups. W/D ratio in LIPC groups is significantly lower. LIPC also could reduce the expression of cytokines and chemokines.
  
  
  CONCLUSIONS
  post conditioning could reduce long-term damages of the lung after lower limb ischemic-reperfusion injury.
authors.affiliation: Center of Cardiac Surgery, Beijing Anzhen Hospital, Capital Medical University, Beijing Institute of Heart, Lung and Blood Vessel Disease Beijing 100029, China.
authors.forename: Shi-Qiu
authors.initials: SQ
authors.lastname: Song
authors.affiliation: Center of Cardiac Surgery, Beijing Anzhen Hospital, Capital Medical University, Beijing Institute of Heart, Lung and Blood Vessel Disease Beijing 100029, China.
authors.forename: Hui-Li
authors.initials: HL
authors.lastname: Gan
authors.affiliation: Center of Cardiac Surgery, Beijing Anzhen Hospital, Capital Medical University, Beijing Institute of Heart, Lung and Blood Vessel Disease Beijing 100029, China.
authors.forename: Jian-Qun
authors.initials: JQ
authors.lastname: Zhang
authors.affiliation: Center of Cardiac Surgery, Beijing Anzhen Hospital, Capital Medical University, Beijing Institute of Heart, Lung and Blood Vessel Disease Beijing 100029, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Feng
authors.affiliation: Center of Cardiac Surgery, Beijing Anzhen Hospital, Capital Medical University, Beijing Institute of Heart, Lung and Blood Vessel Disease Beijing 100029, China.
authors.forename: Jian-Chao
authors.initials: JC
authors.lastname: Sun
authors.affiliation: Center of Cardiac Surgery, Beijing Anzhen Hospital, Capital Medical University, Beijing Institute of Heart, Lung and Blood Vessel Disease Beijing 100029, China.
authors.forename: Sheng-Xun
authors.initials: SX
authors.lastname: Wang
keywords: Ischaemia-reperfusion injury; TNF-α; lung; post-conditioning