title: Quantitative shotgun proteomics reveals extensive changes to the proteome of the orbitofrontal cortex in rats that are hyperactive following withdrawal from a high sugar diet.
abstract: In most Westernized societies, there has been an alarming increase in the consumption of sugar-sweetened drinks. For many adults these drinks represent a substantial proportion of their total daily caloric intake. Here we investigated whether extended exposure to sugar changes behavior and protein expression in the orbitofrontal cortex (OFC). Male adult Sprague-Dawley rats (n = 8 per group) were treated for 26 days with either water or a 10% sucrose solution. Locomotor behavior was measured on the first and last day of treatment, then 1 week after treatment. Following the 1-week period free from treatment, sucrose treated rats were significantly more active than the control. Two hours following final behavioral testing, brains were rapidly removed and prepared for proteomic analysis of the OFC. Label free quantitative shotgun proteomic analyses of three rats from each group found 290 proteins were differentially expressed in the sucrose treated group when compared to the control group. Major changes in the proteome were seen in proteins related to energy metabolism, mitochondrial function and the cellular response to stress. This research does not seek to suggest that sugar will cause specific neurological disorders, however similar changes in proteins have been seen in neurological disorders such as Alzheimer's disease, Parkinson's disease and schizophrenia.
authors.affiliation: Department of Psychology, Macquarie University, Sydney, NSW, Australia.
authors.forename: Jane L
authors.initials: JL
authors.lastname: Franklin
authors.affiliation: Australian School of Advanced Medicine, Macquarie University, Sydney, NSW, Australia.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Mirzaei
authors.affiliation: Department of Psychology, Macquarie University, Sydney, NSW, Australia.
authors.forename: Travis A
authors.initials: TA
authors.lastname: Wearne
authors.affiliation: Department of Psychology, Macquarie University, Sydney, NSW, Australia.
authors.forename: Melanie K
authors.initials: MK
authors.lastname: Sauer
authors.affiliation: Faculty of Human Sciences, Macquarie University, Sydney, NSW, Australia.
authors.forename: Judi
authors.initials: J
authors.lastname: Homewood
authors.affiliation: Australian School of Advanced Medicine, Macquarie University, Sydney, NSW, Australia.
authors.forename: Ann K
authors.initials: AK
authors.lastname: Goodchild
authors.affiliation: Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, NSW, Australia.
authors.forename: Paul A
authors.initials: PA
authors.lastname: Haynes
authors.affiliation: Department of Psychology, Macquarie University, Sydney, NSW, Australia.
authors.forename: Jennifer L
authors.initials: JL
authors.lastname: Cornish
mesh_terms: D000818:Animals; D050260:Carbohydrate Metabolism; D002253:Carbonated Beverages / Q000009:adverse effects; D002149:Energy Intake; D004734:Energy Metabolism; D005947:Glucose / Q000378:metabolism; D008297:Male; D008928:Mitochondria / Q000378:metabolism; D009043:Motor Activity; D018384:Oxidative Stress; D017397:Prefrontal Cortex / Q000378:metabolism* / Q000502:physiology; D020543:Proteome / Q000032:analysis / Q000378:metabolism*; D040901:Proteomics; D051381:Rats; D017207:Rats, Sprague-Dawley; D013395:Sucrose / Q000009:adverse effects / Q000378:metabolism*; D015430:Weight Gain
chemical_list: D020543:Proteome; D013395:Sucrose; D005947:Glucose
keywords: Animal proteomics; Mitochondrial dysfunction; Neurological disorders; Orbitofrontal cortex; Sugar