title: Fluorescence sensor array for identification of commercial milk samples according to their thermal treatments.
abstract: Identification of processed milk is of importance for commercial and legal concerns. The fluorescence response patterns induced by fluorophore/protein interactions allow a possible discrimination of processed milk samples corresponding to their thermal treatment. The fluorescence responses of 4 fluorophores upon addition of commercial milk samples in 96-well plate are measured in the range of 400-600 nm using the excitation wavelength at 375 nm. The pattern recognition of the 53,126 fluorescence responses (4 fluorophores×41 wavelengths×4 thermally processed milks×3 brands×3 lots×3 bottles×3 repeats) are analyzed by multivariate statistical methods. Linear discriminant analysis (LDA) successfully recognizes the milk samples according to their thermal processing, i.e. pasteurized milk, sterilized milk, UHT fresh milk and recombined milk (UHT milk having milk powder), with 100% classification accuracy in a cross validation using a leave-one-out technique.
authors.affiliation: Program in Biotechnology, Faculty of Science, Chulalongkorn University, Phayathai Rd., Bangkok 10330, Thailand.
authors.forename: Radeemada
authors.initials: R
authors.lastname: Mungkarndee
authors.affiliation: Department of Food Technology, Faculty of Science, Ramkhamhaeng University, Bangkok 10240, Thailand.
authors.forename: Ittipon
authors.initials: I
authors.lastname: Techakriengkrai
authors.affiliation: National Nanotechnology Center, National Science and Technology Development Agency, Pathumthani 12120, Thailand.
authors.forename: Gamolwan
authors.initials: G
authors.lastname: Tumcharern
authors.affiliation: Organic Synthesis Unit, Department of Chemistry, Faculty of Science and Nanotec-CU Center of Excellence on Food and Agriculture Chulalongkorn University, Bangkok 10330, Thailand. Electronic address: smongkol@chula.ac.th.
authors.forename: Mongkol
authors.initials: M
authors.lastname: Sukwattanasinitt
mesh_terms: D000818:Animals; D016002:Discriminant Analysis; D005456:Fluorescent Dyes / Q000737:chemistry; D005504:Food Analysis / Q000379:methods*; D005511:Food Handling / Q000379:methods*; D006358:Hot Temperature; D008892:Milk / Q000737:chemistry*; D008894:Milk Proteins / Q000032:analysis*; D015999:Multivariate Analysis; D025341:Principal Component Analysis; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes; D008894:Milk Proteins
keywords: Fluorescence sensor; Linear discriminant analysis (LDA); Milk protein; Multivariate statistical analysis; Principal component analysis (PCA); Protein analysis