title: Haemocyanin content of shrimp (Fenneropenaeus chinensis) associated with white spot syndrome virus and Vibrio harveyi infection process.
abstract: Haemocyanin (Hc) is frequently reported to vary significantly by physiological status and environmental stress in Crustaceans. In this paper, the shrimp Fenneropenaeus chinensis was infected with different concentrations of white spot syndrome virus (WSSV) and Vibrio harveyi. Then, the variation of Hc and total protein content of the haemolymph (TPCH) were investigated using the established double antibody sandwich enzyme linked immunosorbent assay (DAS-ELISA) and Coomassie brilliant blue method, respectively. The results showed that the Hc content peaked at 12 h post-infection (PI) in the 10(-2), 10(-4) and 10(-6) viral supernatant (VS) groups, and the maximum was 93.03 ± 2.55 mg ml(-1), 77.57 ± 6.02 mg ml(-1) and 70.25 ± 3.96 mg ml(-1), respectively. TPCH reached the maximum of 108.18 ± 1.36 mg ml(-1) and 103.49 ± 1.33 mg ml(-1) at 12 h PI in the 10(-2) and 10(-4) VS groups, respectively. The maximum was 96.94 ± 1.06 mg ml(-1) at 24 h PI in the 10(-6) VS group. In the V. harveyi infection groups, the Hc content reached a maximum of 87.97 ± 4.39 mg ml(-1) at 36 h PI in the 10(6) CFU ml(-1) group, 73.74 ± 4.38 mg ml(-1) and 72.47 ± 2.09 mg ml(-1) at 12 h PI in the 10(7) and 10(8) CFU ml(-1) groups, respectively. TPCH reached a maximum of 111.16 ± 0.86 mg ml(-1) at 36 h PI in the 10(6) CFU ml(-1) group, 100.41 ± 0.51 mg ml(-1) and 101.94 ± 0.47 mg ml(-1) at 12 h PI in the 10(7) and 10(8) CFU ml(-1) groups, respectively. These data showed that both Hc content and TPCH varied as the same extent after infection. The up-regulation of the Hc content at 6-36 h PI might be a reference threshold for shrimp infection.
authors.affiliation: Laboratory of Pathology and Immunology of Aquatic Animals, Ocean University of China, Qingdao 266003, PR China.
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Chang
authors.affiliation: Laboratory of Pathology and Immunology of Aquatic Animals, Ocean University of China, Qingdao 266003, PR China. Electronic address: xingjing@ouc.edu.cn.
authors.forename: Jing
authors.initials: J
authors.lastname: Xing
authors.affiliation: Laboratory of Pathology and Immunology of Aquatic Animals, Ocean University of China, Qingdao 266003, PR China.
authors.forename: Xiaoqian
authors.initials: X
authors.lastname: Tang
authors.affiliation: Laboratory of Pathology and Immunology of Aquatic Animals, Ocean University of China, Qingdao 266003, PR China.
authors.forename: Xiuzhen
authors.initials: X
authors.lastname: Sheng
authors.affiliation: Laboratory of Pathology and Immunology of Aquatic Animals, Ocean University of China, Qingdao 266003, PR China; Laboratory for Marine Fisheries and Aquaculture, Qingdao National Laboratory for Marine Science and Technology, No. 1 Wenhai Road, Aoshanwei Town, Jimo, Qingdao, PR China.
authors.forename: Wenbin
authors.initials: W
authors.lastname: Zhan
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D060829:Arthropod Proteins / Q000276:immunology / Q000378:metabolism*; D004266:DNA Virus Infections / Q000378:metabolism* / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay; D005880:Gills / Q000821:virology; D006433:Hemocyanins / Q000276:immunology / Q000378:metabolism*; D006458:Hemolymph / Q000378:metabolism; D033561:Penaeidae / Q000378:metabolism* / Q000382:microbiology / Q000821:virology; D014733:Vibrio; D014735:Vibrio Infections / Q000378:metabolism* / Q000662:veterinary; D046848:White spot syndrome virus 1
chemical_list: D000911:Antibodies, Monoclonal; D060829:Arthropod Proteins; D006433:Hemocyanins
keywords: Double antibody sandwich ELISA; Fenneropenaeus chinensis; Haemocyanin; Vibrio harveyi; White spot syndrome virus