title: Genotyping of 28 blood group alleles in blood donors from Mali: Prediction of rare phenotypes.
abstract: We determined the frequencies of clinically relevant blood group alleles in 300 blood donors from Mali. Multiplex test based on xMAP technology was used to investigate six blood group systems (RH, KEL, MNS, FY, JK, DO, HPA) and complementary analysis were conducted for MNS and RH systems. Polymorphisms that affect the specificity of molecular tests leading to discrepant genotype results are discussed. Antigen expressions were predicted showing that 50% of donors expressed at least one traditional low prevalence antigen, and 11.6% lacked the ability to express at least one high prevalence antigen compatible with Dob-, HPA1a-, S-s-U-, Jsb-, RH:-31 and/or RH:-34 phenotypes.
authors.affiliation: Centre National de Transfusion Sanguine (CNTS), BP E344, Bamako, Mali; Etablissement Français du Sang Alpes Méditerranée, Marseille, France; UMR 7268 ADÉS, Aix-Marseille Université - EFS - CNRS, Marseille, France.
authors.forename: Alhassane
authors.initials: A
authors.lastname: Ba
authors.affiliation: Centre National de Transfusion Sanguine (CNTS), BP E344, Bamako, Mali.
authors.forename: Seydou
authors.initials: S
authors.lastname: Bagayoko
authors.affiliation: Etablissement Français du Sang Alpes Méditerranée, Marseille, France; UMR 7268 ADÉS, Aix-Marseille Université - EFS - CNRS, Marseille, France.
authors.forename: Jacques
authors.initials: J
authors.lastname: Chiaroni
authors.affiliation: Etablissement Français du Sang Alpes Méditerranée, Marseille, France; UMR 7268 ADÉS, Aix-Marseille Université - EFS - CNRS, Marseille, France.
authors.forename: Pascal
authors.initials: P
authors.lastname: Baiily
authors.affiliation: Etablissement Français du Sang Alpes Méditerranée, Marseille, France; UMR 7268 ADÉS, Aix-Marseille Université - EFS - CNRS, Marseille, France. Electronic address: monique.silvy@efs.sante.fr.
authors.forename: Monique
authors.initials: M
authors.lastname: Silvy
mesh_terms: D000483:Alleles; D001782:Blood Donors; D001789:Blood Group Antigens / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D060005:Genotyping Techniques; D006801:Humans; D008297:Male; D008302:Mali; D010641:Phenotype
chemical_list: D001789:Blood Group Antigens
keywords: Africa; Allele frequencies; Genotyping