title: Up-regulation of Proinflammatory Genes and Cytokines Induced by S100A8 in CD8+ T Cells in Lichen Planus.
abstract: Lichen planus (LP) is a chronic inflammatory mucocutaneous disease. The inflammatory status of LP may be related to S100A8 (myeloid-related protein 8; MRP8) activation of cytotoxic cells. The aims of this study were to evaluate S100A8 expression in skin lesions and the in vitro effects of S100A8 on CD8+ T cells and natural killer (NK) cells in LP. Increased levels of S100A8/S100A9 were detected in the skin lesions as well as in the sera of subjects with LP. S100A8 expression induced an increased cytotoxic response by peripheral blood CD8+CD107a+ T cells as well as by NK CD56bright cells in patients with LP. Increased expression of interleukin (IL)-1β, tumour necrosis factor (TNF) and IL-6 in the CD8+ T cells of patients with LP was induced by S100A8, in contrast to the control group that produced IL- 10 and interferon (IFN) type I genes. These data suggest that, in individuals with LP, S100A8 may exert distinct immunomodulatory and cytotoxicity functions.
authors.affiliation: Laboratory of Dermatology and Immunodeficiency (LIM-56), Department of Dermatology, Medical School, University of São Paulo, São Paulo, SP, Brazil.
authors.forename: Gabriel Costa
authors.initials: GC
authors.lastname: de Carvalho
authors.forename: Rosana
authors.initials: R
authors.lastname: Domingues
authors.forename: Marcelle Almeida
authors.initials: MA
authors.lastname: de Sousa Nogueira
authors.forename: Anna C
authors.initials: AC
authors.lastname: Calvielli Castelo Branco
authors.forename: Kelly C
authors.initials: KC
authors.lastname: Gomes Manfrere
authors.forename: Naiura Vieira
authors.initials: NV
authors.lastname: Pereira
authors.forename: Valéria
authors.initials: V
authors.lastname: Aoki
authors.forename: Mirian Nacagami
authors.initials: MN
authors.lastname: Sotto
authors.forename: Alberto J
authors.initials: AJ
authors.lastname: Da Silva Duarte
authors.forename: Maria Notomi
authors.initials: MN
authors.lastname: Sato
mesh_terms: D019002:CD56 Antigen / Q000276:immunology / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D040501:Calgranulin A / Q000276:immunology / Q000378:metabolism*; D040502:Calgranulin B / Q000276:immunology / Q000378:metabolism; D016022:Case-Control Studies; D002478:Cells, Cultured; D016207:Cytokines / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D018836:Inflammation Mediators / Q000276:immunology / Q000378:metabolism*; D007694:Killer Cells, Natural / Q000276:immunology / Q000378:metabolism; D008010:Lichen Planus / Q000235:genetics / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D052118:Lysosomal-Associated Membrane Protein 1 / Q000276:immunology / Q000378:metabolism; D015398:Signal Transduction; D012867:Skin / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D051197:Toll-Like Receptor 4 / Q000276:immunology / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D019002:CD56 Antigen; D040501:Calgranulin A; D040502:Calgranulin B; D016207:Cytokines; D018836:Inflammation Mediators; D052118:Lysosomal-Associated Membrane Protein 1; C522918:NCAM1 protein, human; C495345:TLR4 protein, human; D051197:Toll-Like Receptor 4