title: Involvement of small heat shock proteins, trehalose, and lipids in the thermal stress management in Schizosaccharomyces pombe.
abstract: Changes in the levels of three structurally and functionally different important thermoprotectant molecules, namely small heat shock proteins (sHsps), trehalose, and lipids, have been investigated upon heat shock in Schizosaccharomyces pombe. Both α-crystallin-type sHsps (Hsp15.8 and Hsp16) were induced after prolonged high-temperature treatment but with different kinetic profiles. The shsp null mutants display a weak, but significant, heat sensitivity indicating their importance in the thermal stress management. The heat induction of sHsps is different in wild type and in highly heat-sensitive trehalose-deficient (tps1Δ) cells; however, trehalose level did not show significant alteration in shsp mutants. The altered timing of trehalose accumulation and induction of sHsps suggest that the disaccharide might provide protection at the early stage of the heat stress while elevated amount of sHsps are required at the later phase. The cellular lipid compositions of two different temperature-adapted wild-type S. pombe cells are also altered according to the rule of homeoviscous adaptation, indicating their crucial role in adapting to the environmental temperature changes. Both Hsp15.8 and Hsp16 are able to bind to different lipids isolated from S. pombe, whose interaction might provide a powerful protection against heat-induced damages of the membranes. Our data suggest that all the three investigated thermoprotectant macromolecules play a pivotal role during the thermal stress management in the fission yeast.
authors.affiliation: Institute of Biochemistry, Biological Research Centre, Szeged, Hungary.
authors.forename: Attila
authors.initials: A
authors.lastname: Glatz
authors.affiliation: Institute of Biochemistry, Biological Research Centre, Szeged, Hungary.
authors.forename: Ana-Maria
authors.initials: AM
authors.lastname: Pilbat
authors.affiliation: Institute of Biochemistry, Biological Research Centre, Szeged, Hungary.
authors.forename: Gergely L
authors.initials: GL
authors.lastname: Németh
authors.affiliation: Institute of Biochemistry, Biological Research Centre, Szeged, Hungary.
authors.forename: Katalin
authors.initials: K
authors.lastname: Vince-Kontár
authors.affiliation: Institute of Biochemistry, Biological Research Centre, Szeged, Hungary.
authors.forename: Katalin
authors.initials: K
authors.lastname: Jósvay
authors.affiliation: Institute of Biochemistry, Biological Research Centre, Szeged, Hungary.
authors.forename: Ákos
authors.initials: Á
authors.lastname: Hunya
authors.affiliation: Institute of Biochemistry, Biological Research Centre, Szeged, Hungary.
authors.forename: Andor
authors.initials: A
authors.lastname: Udvardy
authors.affiliation: Institute of Biochemistry, Biological Research Centre, Szeged, Hungary.
authors.forename: Imre
authors.initials: I
authors.lastname: Gombos
authors.affiliation: Institute of Biochemistry, Biological Research Centre, Szeged, Hungary.
authors.forename: Mária
authors.initials: M
authors.lastname: Péter
authors.affiliation: Institute of Biochemistry, Biological Research Centre, Szeged, Hungary.
authors.forename: Gábor
authors.initials: G
authors.lastname: Balogh
authors.affiliation: Institute of Biochemistry, Biological Research Centre, Szeged, Hungary.
authors.forename: Ibolya
authors.initials: I
authors.lastname: Horváth
authors.affiliation: Institute of Biochemistry, Biological Research Centre, Szeged, Hungary.
authors.forename: László
authors.initials: L
authors.lastname: Vígh
authors.affiliation: Institute of Biochemistry, Biological Research Centre, Szeged, Hungary. torok.zsolt@brc.mta.hu.
authors.forename: Zsolt
authors.initials: Z
authors.lastname: Török
mesh_terms: D015966:Gene Expression Regulation, Fungal; D050888:Heat-Shock Proteins, Small / Q000235:genetics / Q000378:metabolism*; D006358:Hot Temperature; D008051:Lipid Bilayers / Q000378:metabolism; D050356:Lipid Metabolism; D009154:Mutation; D012568:Schizosaccharomyces / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics / Q000378:metabolism*; D013312:Stress, Physiological; D014199:Trehalose / Q000235:genetics / Q000378:metabolism*
chemical_list: D050888:Heat-Shock Proteins, Small; D008051:Lipid Bilayers; D029702:Schizosaccharomyces pombe Proteins; D014199:Trehalose
keywords: Fission yeast; Heat stress; Lipids; Membrane; S. pombe; Small Hsps; Trehalose accumulation