title: Chemical Synthesis of Ubiquitin Chains.
abstract: Chemical synthesis of complex biomolecules such as proteins is a challenging adventure, yet rewarding in driving various biochemical and biophysical research activities. Over the years, the refinement of peptide synthesis and invention of ligation methodologies have led to the successful synthesis of several complex protein targets. Ubiquitin bioconjugates, which are being studied intensively by many groups due to their involvement in numerous biological processes, represent a fine example where chemistry is greatly aiding these studies. In this article, we describe the synthetic routes and strategies to prepare different ubiquitin analogs with desired modifications, as well as di-ubiquitin chains.
authors.affiliation: Schulich Faculty of Chemistry, Technion-Israel Institute of Technology, Haifa, Israel.
authors.forename: Hosahalli P
authors.initials: HP
authors.lastname: Hemantha
authors.affiliation: Schulich Faculty of Chemistry, Technion-Israel Institute of Technology, Haifa, Israel.
authors.forename: Somasekhar
authors.initials: S
authors.lastname: Bondalapati
authors.affiliation: Schulich Faculty of Chemistry, Technion-Israel Institute of Technology, Haifa, Israel.
authors.forename: Sumeet K
authors.initials: SK
authors.lastname: Singh
authors.affiliation: Schulich Faculty of Chemistry, Technion-Israel Institute of Technology, Haifa, Israel.
authors.forename: Ashraf
authors.initials: A
authors.lastname: Brik
mesh_terms: D000595:Amino Acid Sequence; D008969:Molecular Sequence Data; D060327:Solid-Phase Synthesis Techniques; D025801:Ubiquitin / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D025801:Ubiquitin
keywords: SPPS; desulfurization; ligation; ubiquitin; ubiquitin chains