title: Paenibacillus radicissp. nov., an endophytic bacterium isolated from maize root.
abstract: A novel Gram-stain-positive, aerobic, endospore-forming, and rod-shaped strain designated 694Twas isolated from surface-sterilized root tissue of a maize planted in the Fangshan District of Beijing, People's Republic of China. A polyphasic taxonomic study was performed on the new isolate. On the basis of 16S rRNA gene sequence similarity studies, this isolate belongs to the genusPaenibacillus. High levels of 16S rRNA gene sequence similarity were found between strain 694TandPaenibacillus xinjiangensisDSM 30034T(98.5 %) andPaenibacillus glycanilyticus(98.1 %), respectively. However, the DNA-DNA hybridization values between strain 694Tand its close relativesP. xinjiangensis16970TandPaenibacillus algorifonticolaCGMCC 1.10223Twere 30.0 % and 36.7 % respectively. The DNA G+C content of strain 694Twas determined to be 46.9 mol%. The predominant respiratory quinone was identified as menaquinone-7 and the polar lipid profile was found to be composed of the major lipids diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The major fatty acids were found to be anteiso-C15 : 0(42.1 %), iso-C15 : 0(18.4 %), iso-C16 : 0(11.2 %) and C16 : 0(12.1 %). The results of physiological and biochemical tests and minor differences in the fatty acid profiles allowed a clear phenotypic differentiation of strain 694Tfrom the closely related species in the genusPaenibacillus.Strain 694Tis concluded to represent a novel species within the genusPaenibacillus, for which the namePaenibacillus radicissp. nov. is proposed, with the type strain 694T( = CGMCC 1.15286T= DSM 100762T).
authors.affiliation: Beijing Agro-Biotechnology Research Center,Beijing Academy of Agriculture and Forestry/Beijing Municipal Key Laboratory of Agricultural Gene Resources and Biotechnology, Beijing 100097, PR China.
authors.forename: Jun-Lian
authors.initials: JL
authors.lastname: Gao
authors.affiliation: Key Laboratory of Microbial Resources,Ministry of Agriculture/Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, PRChina.
authors.forename: Mei
authors.initials: M
authors.lastname: Yuan
authors.affiliation: Beijing Agro-Biotechnology Research Center,Beijing Academy of Agriculture and Forestry/Beijing Municipal Key Laboratory of Agricultural Gene Resources and Biotechnology, Beijing 100097, PR China.
authors.forename: Xu-Ming
authors.initials: XM
authors.lastname: Wang
authors.affiliation: Beijing Agro-Biotechnology Research Center,Beijing Academy of Agriculture and Forestry/Beijing Municipal Key Laboratory of Agricultural Gene Resources and Biotechnology, Beijing 100097, PR China.
authors.forename: Tian-Lei
authors.initials: TL
authors.lastname: Qiu
authors.affiliation: Key Laboratory of Microbial Resources,Ministry of Agriculture/Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, PRChina.
authors.forename: Fan-Yang
authors.initials: FY
authors.lastname: Lv
authors.affiliation: Beijing Agro-Biotechnology Research Center,Beijing Academy of Agriculture and Forestry/Beijing Municipal Key Laboratory of Agricultural Gene Resources and Biotechnology, Beijing 100097, PR China.|College of Agriculture,Yangtze University, Hubei Jingzhou 434025, PRChina.
authors.forename: Ming-Ming
authors.initials: MM
authors.lastname: Yang
authors.affiliation: Key Laboratory of Microbial Resources,Ministry of Agriculture/Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, PRChina.
authors.forename: Jian-Guang
authors.initials: JG
authors.lastname: Sun