title: Diagnostic value of serum Golgi protein 73 for HBV-related primary hepatic carcinoma.
abstract: BACKGROUND
  Alpha-fetoprotein (AFP) levels are routinely used for diagnosis and monitoring of hepatic diseases, but it has a limited value. Golgi protein 73 (GP73) has been suggested as a new marker for hepatic diseases.
  
  
  OBJECTIVE
  To explore the clinical value of serum GP73 in different diseases associated with hepatitis B virus (HBV) infection.
  
  
  METHODS
  Between January 2010 and August 2014, serum samples from 88 patients with chronic hepatitis B (CHB), 78 patients with HBV-related liver cirrhosis (LC), and 194 patients with HBV-related primary hepatic cancer (PHC) were collected. Serum samples from 30 healthy volunteers were used as controls. ELISA and microparticle enzyme immunoassay were used to measure serum GP73 and AFP levels. Receiver operating characteristic (ROC) curves were used to analyze the diagnostic value of serum GP73 and AFP for PHC.
  
  
  RESULTS
  For the diagnosis of PHC, GP73 showed a sensitivity of 65.5% and specificity of 66.3%, while AFP levels showed sensitivity of 64.4% and specificity of 76.5%. Serial testing (both tests are positive) could increase the specificity (sensitivity of 45.9% and specificity of 85.5%) while parallel testing (any single positive test result) could increase the sensitivity (sensitivity of 84.0% and specificity of 57.2%). Serum GP73 and AFP levels were significantly different between Child-Pugh grades (P<0.001 for GP73 and P=0.044 for AFP). Significant differences in serum GP73 and AFP were found between TNM stages (all P<0.001).
  
  
  CONCLUSIONS
  Serum GP73 had limited diagnostic value for HBV-related PHC. The combined use of serum GP73 and AFP levels improved the diagnostic efficacy.
authors.affiliation: Department of Clinical Laboratory, Ningbo No. 2 Hospital Ningbo, Zhejiang, China.
authors.forename: Guosheng
authors.initials: G
authors.lastname: Gao
authors.affiliation: Department of Clinical Laboratory, Ningbo No. 2 Hospital Ningbo, Zhejiang, China.
authors.forename: Feibo
authors.initials: F
authors.lastname: Dong
authors.affiliation: Liver Disease Branch, Ningbo No. 2 Hospital Ningbo, Zhejiang, China.
authors.forename: Xiaozhen
authors.initials: X
authors.lastname: Xu
authors.affiliation: Liver Disease Branch, Ningbo No. 2 Hospital Ningbo, Zhejiang, China.
authors.forename: Airong
authors.initials: A
authors.lastname: Hu
authors.affiliation: Liver Disease Branch, Ningbo No. 2 Hospital Ningbo, Zhejiang, China.
authors.forename: Yaoren
authors.initials: Y
authors.lastname: Hu
mesh_terms: D000328:Adult; D000368:Aged; D019540:Area Under Curve; D014408:Biomarkers, Tumor / Q000097:blood*; D006528:Carcinoma, Hepatocellular / Q000097:blood* / Q000175:diagnosis / Q000821:virology; D016022:Case-Control Studies; D003430:Cross-Sectional Studies; D005260:Female; D006509:Hepatitis B / Q000150:complications* / Q000175:diagnosis; D006801:Humans; D008113:Liver Neoplasms / Q000097:blood* / Q000175:diagnosis / Q000821:virology; D008297:Male; D008565:Membrane Proteins / Q000097:blood*; D008875:Middle Aged; D009367:Neoplasm Staging; D011237:Predictive Value of Tests; D012372:ROC Curve; D015203:Reproducibility of Results; D000509:alpha-Fetoproteins / Q000032:analysis
chemical_list: C097353:AFP protein, human; D014408:Biomarkers, Tumor; C410824:GOLM1 protein, human; D008565:Membrane Proteins; D000509:alpha-Fetoproteins
keywords: Golgi protein 73; Hepatitis B virus; alpha fetoprotein; primary hepatic carcinoma