title: Human Blastocyst Secreted microRNA Regulate Endometrial Epithelial Cell Adhesion.
abstract: Successful embryo implantation requires synchronous development and communication between the blastocyst and the endometrium, however the mechanisms of communication in humans are virtually unknown. Recent studies have revealed that microRNAs (miRs) are present in bodily fluids and secreted by cells in culture. We have identified that human blastocysts differentially secrete miRs in a pattern associated with their implantation outcome. miR-661 was the most highly expressed miR in blastocyst culture media (BCM) from blastocysts that failed to implant (non-implanted) compared to blastocysts that implanted (implanted). Our results indicate a possible role for Argonaute 1 in the transport of miR-661 in non-implanted BCM and taken up by primary human endometrial epithelial cells (HEECs). miR-661 uptake by HEEC reduced trophoblast cell line spheroid attachment to HEEC via PVRL1. Our results suggest that human blastocysts alter the endometrial epithelial adhesion, the initiating event of implantation, via the secretion of miR, abnormalities in which result in implantation failure.
authors.affiliation: Embryo Implantation Laboratory, Hudson Institute, Clayton, Victoria 3168 Australia ; Monash IVF, Monash Surgical Private Hospital, Clayton, Victoria 3168, Australia ; Department of Molecular and Translational Science, Monash University, Melbourne, Australia.
authors.forename: Carly
authors.initials: C
authors.lastname: Cuman
authors.affiliation: Embryo Implantation Laboratory, Hudson Institute, Clayton, Victoria 3168 Australia ; Department of Molecular and Translational Science, Monash University, Melbourne, Australia.
authors.forename: Michelle
authors.initials: M
authors.lastname: Van Sinderen
authors.affiliation: Department of Molecular and Translational Science, Monash University, Melbourne, Australia ; Centre for Cancer Research, Hudson Institute, Clayton, Victoria 3168 Australia.
authors.forename: Michael P
authors.initials: MP
authors.lastname: Gantier
authors.affiliation: Embryo Implantation Laboratory, Hudson Institute, Clayton, Victoria 3168 Australia.
authors.forename: Kate
authors.initials: K
authors.lastname: Rainczuk
authors.affiliation: Embryo Implantation Laboratory, Hudson Institute, Clayton, Victoria 3168 Australia ; Monash IVF, Monash Surgical Private Hospital, Clayton, Victoria 3168, Australia ; Department of Anatomy and Developmental Biology, Monash University, Victoria 3800, Australia.
authors.forename: Kelli
authors.initials: K
authors.lastname: Sorby
authors.affiliation: Monash IVF, Monash Surgical Private Hospital, Clayton, Victoria 3168, Australia ; Department of Obstetrics and Gynaecology, Monash University, Victoria 3800, Australia.
authors.forename: Luk
authors.initials: L
authors.lastname: Rombauts
authors.affiliation: Monash IVF, Monash Surgical Private Hospital, Clayton, Victoria 3168, Australia ; Department of Obstetrics and Gynaecology, Monash University, Victoria 3800, Australia.
authors.forename: Tiki
authors.initials: T
authors.lastname: Osianlis
authors.affiliation: Embryo Implantation Laboratory, Hudson Institute, Clayton, Victoria 3168 Australia ; Department of Anatomy and Developmental Biology, Monash University, Victoria 3800, Australia.
authors.forename: Evdokia
authors.initials: E
authors.lastname: Dimitriadis
mesh_terms: D060565:Argonaute Proteins / Q000378:metabolism; D001755:Blastocyst / Q000378:metabolism*; D002448:Cell Adhesion / Q000235:genetics; D015815:Cell Adhesion Molecules / Q000235:genetics; D002460:Cell Line; D010064:Embryo Implantation / Q000235:genetics; D004717:Endometrium / Q000166:cytology* / Q000378:metabolism*; D004847:Epithelial Cells / Q000378:metabolism*; D039642:Eukaryotic Initiation Factors / Q000378:metabolism; D005260:Female; D005307:Fertilization in Vitro; D006655:Histone Deacetylases / Q000235:genetics; D006801:Humans; D035683:MicroRNAs / Q000737:chemistry / Q000235:genetics*; D000075983:Nectins; D034622:RNA Interference; D034443:RNA Transport; D012097:Repressor Proteins / Q000235:genetics
chemical_list: C115848:AGO1 protein, human; D060565:Argonaute Proteins; D015815:Cell Adhesion Molecules; D039642:Eukaryotic Initiation Factors; C543810:MIRN661 microRNA, human; D035683:MicroRNAs; C000619010:NECTIN1 protein, human; D000075983:Nectins; D012097:Repressor Proteins; C120917:MTA2 protein, human; D006655:Histone Deacetylases
keywords: Embryo; Endometrium; Implantation; miR-661; microRNA