title: Molecular identification and characterization of prohibitin from Echinococcus granulosus.
abstract: Prohibitin (PHB) is a widely distributed protein that functions as a molecular chaperone, is involved in the regulation of cell cycle, and maintains mitochondrial structure and functions of the anti-apoptosis, senescence, and proliferation. The aim of this study was to characterize PHB in Echinococcus granulosus (EgPHB), a harmful cestode parasite of humans, many livestock species, and wild animals. We found that EgPHB is a conserved SPFH (stomatin, prohibitin, flotillin, and HflK/C) domain-containing protein, consisting of 289 amino acids, which shares 42.66-99.31% identity with PHBs from other parasites and mammals. EgPHB was located mainly in the tegument issue of protoscoleces, in the inner body of adult worms, and was expressed widely in the germinal layer. This is the first report on prohibitin from E. granulosus, and EgPHB is considered to be a valuable protein to study more in the future.
authors.affiliation: Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Huimin Road, 211#, Chengdu, 611130, Sichuan Province, China.
authors.forename: Xiuqin
authors.initials: X
authors.lastname: Zhong
authors.affiliation: Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Huimin Road, 211#, Chengdu, 611130, Sichuan Province, China.
authors.forename: Xingju
authors.initials: X
authors.lastname: Song
authors.affiliation: Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Huimin Road, 211#, Chengdu, 611130, Sichuan Province, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Wang
authors.affiliation: Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Huimin Road, 211#, Chengdu, 611130, Sichuan Province, China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Hu
authors.affiliation: Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Huimin Road, 211#, Chengdu, 611130, Sichuan Province, China.
authors.forename: Tinayu
authors.initials: T
authors.lastname: Liu
authors.affiliation: Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Huimin Road, 211#, Chengdu, 611130, Sichuan Province, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.affiliation: Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Huimin Road, 211#, Chengdu, 611130, Sichuan Province, China.
authors.forename: Xiaobin
authors.initials: X
authors.lastname: Gu
authors.affiliation: Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Huimin Road, 211#, Chengdu, 611130, Sichuan Province, China.
authors.forename: Weimin
authors.initials: W
authors.lastname: Lai
authors.affiliation: Department of Chemistry, College of Life and Basic Science, Sichuan Agricultural University, Ya'an, China.
authors.forename: Xuerong
authors.initials: X
authors.lastname: Peng
authors.affiliation: Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Huimin Road, 211#, Chengdu, 611130, Sichuan Province, China. guangyou1963@aliyun.com.
authors.forename: Guangyou
authors.initials: G
authors.lastname: Yang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004443:Echinococcosis / Q000469:parasitology*; D048209:Echinococcus granulosus / Q000166:cytology / Q000235:genetics* / Q000378:metabolism; D015801:Helminth Proteins / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D000091402:Prohibitins; D000072417:Protein Domains; D011817:Rabbits; D012097:Repressor Proteins / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D015801:Helminth Proteins; C000717328:PHB protein, human; D000091402:Prohibitins; D012097:Repressor Proteins
keywords: Echinococcus granulosus; Immunohistochemical localization; Prohibitin; Western blotting