title: Nrdp1S, short variant of Nrdp1, inhibits human glioma progression by increasing Nrdp1-mediated ErbB3 ubiquitination and degradation.
abstract: The ubiquitin ligase neuregulin receptor degradation protein 1 (Nrdp1) is involved in the induction of apoptosis and suppression of tumour formation. We previously showed that it was expressed at lower levels in human glioma tissues compared with normal brain tissues. However, the mechanism underlying this is unclear. Here, we reported that a novel short variant (Nrdp1S), lacking 71 amino acids at the N-terminal, was expressed in normal human brain tissue, but absent from glioma tissues. Similar to Nrdp1, Nrdp1S could be degraded by the proteasomal pathway, but exhibited an even longer half-life than Nrdp1. Nrdp1S was also shown to form a heterodimer with Nrdp1, which increased its stability, thereby augmenting the Nrdp1-mediated ubiquitination and degradation of ErbB3. EdU incorporation, MTT assay and in vitro colony formation demonstrated that Nrdp1S significantly inhibited the cell tumourigenicity. These results together suggest that Nrdp1S is a tumour suppressor that which potentiates the Nrdp1-mediated ubiquitination and degradation of ErbB3. An Nrdp1S deficiency may also be an important factor in the loss of Nrdp1.
authors.affiliation: Insititute of Nervous System Diseases, Xuzhou Medical College, Xuzhou, China.|The School of Clinical Medicine, Xuzhou Medical College, Xuzhou, China.|The Graduate School, Xuzhou Medical College, Xuzhou, China.
authors.forename: Yuxuan
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Insititute of Nervous System Diseases, Xuzhou Medical College, Xuzhou, China.|Brain Hospital, Affiliated Hospital of Xuzhou Medical College, Xuzhou, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: Insititute of Nervous System Diseases, Xuzhou Medical College, Xuzhou, China.|The Graduate School, Xuzhou Medical College, Xuzhou, China.
authors.forename: Hanmo
authors.initials: H
authors.lastname: Bao
authors.affiliation: Insititute of Nervous System Diseases, Xuzhou Medical College, Xuzhou, China.|The Graduate School, Xuzhou Medical College, Xuzhou, China.
authors.forename: Shenshan
authors.initials: S
authors.lastname: Zou
authors.affiliation: Institute of Blood Diseases, Xuzhou Medical College, Xuzhou, China.
authors.forename: Chunling
authors.initials: C
authors.lastname: Fu
authors.affiliation: Insititute of Nervous System Diseases, Xuzhou Medical College, Xuzhou, China.|The Graduate School, Xuzhou Medical College, Xuzhou, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Gong
authors.affiliation: Insititute of Nervous System Diseases, Xuzhou Medical College, Xuzhou, China.|The Graduate School, Xuzhou Medical College, Xuzhou, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Insititute of Nervous System Diseases, Xuzhou Medical College, Xuzhou, China.|The Graduate School, Xuzhou Medical College, Xuzhou, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Tang
authors.affiliation: Insititute of Nervous System Diseases, Xuzhou Medical College, Xuzhou, China.|Brain Hospital, Affiliated Hospital of Xuzhou Medical College, Xuzhou, China.
authors.forename: Rutong
authors.initials: R
authors.lastname: Yu
authors.affiliation: Insititute of Nervous System Diseases, Xuzhou Medical College, Xuzhou, China.|Brain Hospital, Affiliated Hospital of Xuzhou Medical College, Xuzhou, China.
authors.forename: Hengliang
authors.initials: H
authors.lastname: Shi
mesh_terms: D001483:Base Sequence; D001932:Brain Neoplasms / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D018450:Disease Progression; D005910:Glioma / Q000378:metabolism* / Q000473:pathology; D057809:HEK293 Cells; D006801:Humans; D020033:Protein Isoforms / Q000502:physiology; D055550:Protein Stability; D059748:Proteolysis; D020893:Receptor, ErbB-3 / Q000378:metabolism*; D044767:Ubiquitin-Protein Ligases / Q000502:physiology*; D054875:Ubiquitination
chemical_list: D020033:Protein Isoforms; C452580:RNF41 protein, human; D044767:Ubiquitin-Protein Ligases; C581292:ERBB3 protein, human; D020893:Receptor, ErbB-3
keywords: ErbB3; Nrdp1; Nrdp1S; glioma; ubiquitination