title: A composite scaffold of MSC affinity peptide-modified demineralized bone matrix particles and chitosan hydrogel for cartilage regeneration.
abstract: Articular cartilage injury is still a significant challenge because of the poor intrinsic healing potential of cartilage. Stem cell-based tissue engineering is a promising technique for cartilage repair. As cartilage defects are usually irregular in clinical settings, scaffolds with moldability that can fill any shape of cartilage defects and closely integrate with the host cartilage are desirable. In this study, we constructed a composite scaffold combining mesenchymal stem cells (MSCs) E7 affinity peptide-modified demineralized bone matrix (DBM) particles and chitosan (CS) hydrogel for cartilage engineering. This solid-supported composite scaffold exhibited appropriate porosity, which provided a 3D microenvironment that supports cell adhesion and proliferation. Cell proliferation and DNA content analysis indicated that the DBM-E7/CS scaffold promoted better rat bone marrow-derived MSCs (BMMSCs) survival than the CS or DBM/CS groups. Meanwhile, the DBM-E7/CS scaffold increased matrix production and improved chondrogenic differentiation ability of BMMSCs in vitro. Furthermore, after implantation in vivo for four weeks, compared to those in control groups, the regenerated issue in the DBM-E7/CS group exhibited translucent and superior cartilage-like structures, as indicated by gross observation, histological examination, and assessment of matrix staining. Overall, the functional composite scaffold of DBM-E7/CS is a promising option for repairing irregularly shaped cartilage defects.
authors.affiliation: Institute of Sports Medicine, Beijing Key Laboratory of Sports Injuries, Peking University Third Hospital, 49 North Garden Road, Haidian District, Beijing 100191, PR China.
authors.forename: Qingyang
authors.initials: Q
authors.lastname: Meng
authors.affiliation: Institute of Sports Medicine, Beijing Key Laboratory of Sports Injuries, Peking University Third Hospital, 49 North Garden Road, Haidian District, Beijing 100191, PR China.
authors.forename: Zhentao
authors.initials: Z
authors.lastname: Man
authors.affiliation: Institute of Sports Medicine, Beijing Key Laboratory of Sports Injuries, Peking University Third Hospital, 49 North Garden Road, Haidian District, Beijing 100191, PR China.
authors.forename: Linghui
authors.initials: L
authors.lastname: Dai
authors.affiliation: Institute of Sports Medicine, Beijing Key Laboratory of Sports Injuries, Peking University Third Hospital, 49 North Garden Road, Haidian District, Beijing 100191, PR China.
authors.forename: Hongjie
authors.initials: H
authors.lastname: Huang
authors.affiliation: Institute of Sports Medicine, Beijing Key Laboratory of Sports Injuries, Peking University Third Hospital, 49 North Garden Road, Haidian District, Beijing 100191, PR China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Institute of Sports Medicine, Beijing Key Laboratory of Sports Injuries, Peking University Third Hospital, 49 North Garden Road, Haidian District, Beijing 100191, PR China.
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Hu
authors.affiliation: Institute of Sports Medicine, Beijing Key Laboratory of Sports Injuries, Peking University Third Hospital, 49 North Garden Road, Haidian District, Beijing 100191, PR China.
authors.forename: Zhenxing
authors.initials: Z
authors.lastname: Shao
authors.affiliation: Institute of Sports Medicine, Beijing Key Laboratory of Sports Injuries, Peking University Third Hospital, 49 North Garden Road, Haidian District, Beijing 100191, PR China.
authors.forename: Jingxian
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Institute of Sports Medicine, Beijing Key Laboratory of Sports Injuries, Peking University Third Hospital, 49 North Garden Road, Haidian District, Beijing 100191, PR China.
authors.forename: Jiying
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Institute of Sports Medicine, Beijing Key Laboratory of Sports Injuries, Peking University Third Hospital, 49 North Garden Road, Haidian District, Beijing 100191, PR China.
authors.forename: Xin
authors.initials: X
authors.lastname: Fu
authors.affiliation: Institute of Sports Medicine, Beijing Key Laboratory of Sports Injuries, Peking University Third Hospital, 49 North Garden Road, Haidian District, Beijing 100191, PR China.
authors.forename: Xiaoning
authors.initials: X
authors.lastname: Duan
authors.affiliation: Institute of Sports Medicine, Beijing Key Laboratory of Sports Injuries, Peking University Third Hospital, 49 North Garden Road, Haidian District, Beijing 100191, PR China.
authors.forename: Yingfang
authors.initials: Y
authors.lastname: Ao
mesh_terms: D000818:Animals; D001857:Bone Matrix / Q000737:chemistry* / Q000378:metabolism; D002358:Cartilage, Articular / Q000502:physiology*; D049109:Cell Proliferation; D048271:Chitosan / Q000737:chemistry; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry* / Q000494:pharmacology; D008422:Materials Testing / Q000379:methods; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology; D008819:Mice, Nude; D017207:Rats, Sprague-Dawley; D012038:Regeneration / Q000502:physiology*; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds
chemical_list: D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D048271:Chitosan