title: Protective role of E. coli outer membrane vesicles against antibiotics.
abstract: The outer membrane vesicles (OMVs) from bacteria are known to posses both defensive and protective functions and thus participate in community related functions. In the present study, outer membrane vesicles have been shown to protect the producer bacterium and two other bacterial species from the growth inhibitory effects of some antibiotics. The OMVs isolated from E. coli MG1655 protected the bacteria against membrane-active antibiotics colistin, melittin. The OMVs of E. coli MG1655 could also protect P. aeruginosa NCTC6751 and A. radiodioresistens MMC5 against these membrane-active antibiotics. However, OMVs could not protect any of these bacteria against the other antibiotics ciprofloxacin, streptomycin and trimethoprim. Hence, OMVs appears to protect the bacterial community against membrane-active antibiotics and not other antibiotics, which have different mechanism of actions. The OMVs of E. coli MG1655 sequester the antibiotic colistin, whereas their protein components degrade the antimicrobial peptide melittin. Proteomic analysis of OMVs revealed the presence of proteases and peptidases which appear to be involved in this process. Thus, the protection of bacteria by OMVs against antibiotics is situation dependent and the mechanism differs for different situations. These studies suggest that OMVs of bacteria form a common defense for the bacterial community against specific antibiotics.
authors.affiliation: CSIR- Centre for Cellular and Molecular Biology, Tarnaka, Hyderabad 500007, India.
authors.forename: Heramb M
authors.initials: HM
authors.lastname: Kulkarni
authors.affiliation: CSIR- Centre for Cellular and Molecular Biology, Tarnaka, Hyderabad 500007, India.
authors.forename: R
authors.initials: R
authors.lastname: Nagaraj
authors.affiliation: CSIR- Centre for Cellular and Molecular Biology, Tarnaka, Hyderabad 500007, India. Electronic address: jagan@ccmb.res.in.
authors.forename: Medicharla V
authors.initials: MV
authors.lastname: Jagannadham
mesh_terms: D000150:Acinetobacter / Q000187:drug effects; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001425:Bacterial Outer Membrane Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D002939:Ciprofloxacin / Q000494:pharmacology; D003091:Colistin / Q000494:pharmacology; D024881:Drug Resistance, Bacterial; D004926:Escherichia coli / Q000737:chemistry / Q000187:drug effects* / Q000254:growth & development; D008555:Melitten / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D046529:Microscopy, Electron, Transmission; D040901:Proteomics; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D013307:Streptomycin / Q000494:pharmacology; D014295:Trimethoprim / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D001425:Bacterial Outer Membrane Proteins; D008555:Melitten; D002939:Ciprofloxacin; D014295:Trimethoprim; D013307:Streptomycin; D003091:Colistin
keywords: Antibiotic resistance; Common defense; E. coli; MALDI TOF/TOF; Outer membrane vesicles; Proteomics