title: In vitro follicle growth supports human oocyte meiotic maturation.
abstract: In vitro follicle growth is a potential approach to preserve fertility for young women who are facing a risk of premature ovarian failure (POF) caused by radiation or chemotherapy. Our two-step follicle culture strategy recapitulated the dynamic human follicle growth environment in vitro. Follicles developed from the preantral to antral stage, and, for the first time, produced meiotically competent metaphase II (MII) oocytes after in vitro maturation (IVM).
authors.affiliation: Department of Obstetrics and Gynecology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA.|Center for Reproductive Science, Northwestern University, Evanston, IL 60208, USA.
authors.forename: Shuo
authors.initials: S
authors.lastname: Xiao
authors.affiliation: Department of Obstetrics and Gynecology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA.|Master of Biotechnology Program, Northwestern University, Evanston, IL 60208, USA.
authors.forename: Jiyang
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Obstetrics and Gynecology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA.|Center for Reproductive Science, Northwestern University, Evanston, IL 60208, USA.
authors.forename: Megan M
authors.initials: MM
authors.lastname: Romero
authors.affiliation: Northwestern Medical Group, Northwestern University, Chicago, IL, 60611, USA.
authors.forename: Kristin N
authors.initials: KN
authors.lastname: Smith
authors.affiliation: Department of Biomedical Engineering, College of Engineering and Medical School, University of Michigan, Ann Arbor, MI 48109, USA.
authors.forename: Lonnie D
authors.initials: LD
authors.lastname: Shea
authors.affiliation: Department of Obstetrics and Gynecology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA.|Center for Reproductive Science, Northwestern University, Evanston, IL 60208, USA.
authors.forename: Teresa K
authors.initials: TK
authors.lastname: Woodruff
mesh_terms: D000328:Adult; D000464:Alginates / Q000737:chemistry; D054304:Anti-Mullerian Hormone / Q000096:biosynthesis / Q000378:metabolism; D004958:Estradiol / Q000096:biosynthesis / Q000378:metabolism; D005260:Female; D005307:Fertilization in Vitro; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D020100:Hydrogels / Q000737:chemistry; D008677:Metaphase; D009865:Oocytes / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism; D006080:Ovarian Follicle / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism; D016649:Primary Ovarian Insufficiency / Q000517:prevention & control; D011374:Progesterone / Q000096:biosynthesis / Q000378:metabolism; D046509:Tissue Culture Techniques / Q000295:instrumentation / Q000379:methods*
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D020100:Hydrogels; D011374:Progesterone; D004958:Estradiol; D054304:Anti-Mullerian Hormone; D020723:Glucuronic Acid