title: Toenails as an alternative source material for the extraction of DNA from decomposed human remains.
abstract: The DNA identification of decomposed human remains for coronial investigations at the Victorian Institute of Forensic Medicine routinely requires the retrieval and processing of a bone sample obtained from the deceased. Bone is a difficult sample type to work with as it requires surgical removal from the deceased, refrigerated storage, and additional processing steps prior to DNA analysis in comparison to other samples types such as buccal swabs or blood stains. In an attempt to overcome the issues posed by bone, a DNA extraction method utilising toenails as an alternate source material was optimised and trialled. Two DNA extraction methods were optimised for digestion of toenail material, with the method utilising the QIAGEN DNA Investigator Kit selected for a casework trial. Single source DNA profiles, matching those of the conventional samples taken, were obtained for toenail samples collected from 28 of 30 coronial cases available for this study. Of these, 26 toenail samples produced full profiles. Although the overall DNA profile quality from the toenails was less than that of the conventional sample, the profiles from toenails met the reporting requirements for identification. Based on the results obtained, the Victorian Institute of Forensic Medicine will be implementing toenails as the primary sample type for collection from decomposed remains when blood is not a suitable sample type.
authors.affiliation: Victorian Institute of Forensic Medicine, 65 Kavanagh St, Southbank, VIC 3006, Australia.
authors.forename: Andrew
authors.initials: A
authors.lastname: Schlenker
authors.affiliation: Victorian Institute of Forensic Medicine, 65 Kavanagh St, Southbank, VIC 3006, Australia.
authors.forename: Katelyn
authors.initials: K
authors.lastname: Grimble
authors.affiliation: Victorian Institute of Forensic Medicine, 65 Kavanagh St, Southbank, VIC 3006, Australia.
authors.forename: Arani
authors.initials: A
authors.lastname: Azim
authors.affiliation: Victorian Institute of Forensic Medicine, 65 Kavanagh St, Southbank, VIC 3006, Australia.
authors.forename: Rebecca
authors.initials: R
authors.lastname: Owen
authors.affiliation: Victorian Institute of Forensic Medicine, 65 Kavanagh St, Southbank, VIC 3006, Australia; Department of Forensic Medicine, Monash University, Australia. Electronic address: dadna.hartman@vifm.org.
authors.forename: Dadna
authors.initials: D
authors.lastname: Hartman
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D004247:DNA / Q000302:isolation & purification*; D016172:DNA Fingerprinting; D005260:Female; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats; D008875:Middle Aged; D009262:Nails / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D011180:Postmortem Changes; D014034:Toes
chemical_list: D004247:DNA
keywords: DNA profiling; DVI; Decomposed human remains; Forensic biology; Human identification; Toenail