title: CD147 monoclonal antibody mediated by chitosan nanoparticles loaded with α-hederin enhances antineoplastic activity and cellular uptake in liver cancer cells.
abstract: An antibody that specifically interacts with an antigen could be applied to an active targeting delivery system. In this study, CD147 antibody was coupled with α-hed chitosan nanoparticles (α-Hed-CS-NPs). α-Hed-CS-CD147-NPs were round and spherical in shape, with an average particle size of 148.23 ± 1.75 nm. The half-maximum inhibiting concentration (IC50) of α-Hed-CS-CD147-NPs in human liver cancer cell lines HepG2 and SMMC-7721 was lower than that of free α-Hed and α-Hed-CS-NPs. α-Hed-induced cell death was mainly triggered by apoptosis. The increase in intracellular accumulation of α-Hed-CS-CD147-NPs was also related to CD147-mediated internalization through the Caveolae-dependent pathway and lysosomal escape. The higher targeting antitumor efficacy of α-Hed-CS-CD147-NPs than that α-Hed-CS-NPs was attributed to its stronger fluorescence intensity in the tumor site in nude mice.
authors.affiliation: Department of Pharmaceutics, College of Pharmaceutical Sciences, Soochow University, Suzhou 215123, People's Republic of China.
authors.forename: Rong
authors.initials: R
authors.lastname: Zhu
authors.affiliation: Department of Pharmaceutics, College of Pharmaceutical Sciences, Soochow University, Suzhou 215123, People's Republic of China.
authors.forename: Chun-ge
authors.initials: CG
authors.lastname: Zhang
authors.affiliation: Department of Pharmaceutics, College of Pharmaceutical Sciences, Soochow University, Suzhou 215123, People's Republic of China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Pharmaceutics, College of Pharmaceutical Sciences, Soochow University, Suzhou 215123, People's Republic of China.
authors.forename: Zhi-qiang
authors.initials: ZQ
authors.lastname: Yuan
authors.affiliation: Department of Pharmaceutics, College of Pharmaceutical Sciences, Soochow University, Suzhou 215123, People's Republic of China.
authors.forename: Wei-liang
authors.initials: WL
authors.lastname: Chen
authors.affiliation: Department of Pharmaceutics, College of Pharmaceutical Sciences, Soochow University, Suzhou 215123, People's Republic of China.
authors.forename: Shu-di
authors.initials: SD
authors.lastname: Yang
authors.affiliation: Department of Pharmaceutics, College of Pharmaceutical Sciences, Soochow University, Suzhou 215123, People's Republic of China.
authors.forename: Ji-zhao
authors.initials: JZ
authors.lastname: Li
authors.affiliation: Department of Pharmaceutics, College of Pharmaceutical Sciences, Soochow University, Suzhou 215123, People's Republic of China.
authors.forename: Wen-jing
authors.initials: WJ
authors.lastname: Zhu
authors.affiliation: College of Radiological Medicine and Protection, Soochow University, Suzhou 215123, People's Republic of China.|Changshu Hospital of Traditional Chinese Medicine, Changshu 215500, People's Republic of China.
authors.forename: Xiao-feng
authors.initials: XF
authors.lastname: Zhou
authors.affiliation: Department of Pharmaceutics, College of Pharmaceutical Sciences, Soochow University, Suzhou 215123, People's Republic of China.
authors.forename: Ben-gang
authors.initials: BG
authors.lastname: You
authors.affiliation: Department of Pharmaceutics, College of Pharmaceutical Sciences, Soochow University, Suzhou 215123, People's Republic of China.
authors.forename: Xue-nong
authors.initials: XN
authors.lastname: Zhang
mesh_terms: D000818:Animals; D017304:Annexin A5 / Q000378:metabolism; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D051926:Basigin / Q000276:immunology*; D048271:Chitosan / Q000737:chemistry*; D004705:Endocytosis / Q000187:drug effects*; D005434:Flow Cytometry; D005453:Fluorescence; D056945:Hep G2 Cells; D006801:Humans; D021621:Imaging, Three-Dimensional; D042541:Intracellular Space / Q000378:metabolism; D008113:Liver Neoplasms / Q000473:pathology*; D008819:Mice, Nude; D018613:Microscopy, Confocal; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D010316:Particle Size; D011419:Propidium / Q000378:metabolism; D012503:Saponins / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D017550:Spectroscopy, Fourier Transform Infrared; D019265:Spectroscopy, Near-Infrared; D013347:Subcellular Fractions / Q000378:metabolism
chemical_list: D017304:Annexin A5; D000911:Antibodies, Monoclonal; D000970:Antineoplastic Agents; D012503:Saponins; C010177:beta-hederin; D051926:Basigin; D011419:Propidium; D009828:Oleanolic Acid; D048271:Chitosan