title: Long Non-Coding RNA MEG3 Inhibits Cell Proliferation and Induces Apoptosis in Prostate Cancer.
abstract: OBJECTIVE
  Long non-coding RNAs (lncRNAs) play important roles in diverse biological processes, such as cell growth, apoptosis and migration. Although downregulation of lncRNA maternally expressed gene 3 (MEG3) has been identified in several cancers, little is known about its role in prostate cancer progression. The aim of this study was to detect MEG3 expression in clinical prostate cancer tissues, investigate its biological functions in the development of prostate cancer and the underlying mechanism.
  
  
  METHODS
  MEG3 expression levels were detected by qRT-PCR in both tumor tissues and adjacent non-tumor tissues from 21 prostate cancer patients. The effects of MEG3 on PC3 and DU145 cells were assessed by MTT assay, colony formation assay, western blot and flow cytometry. Transfected PC3 cells were transplanted into nude mice, and the tumor growth curves were determined.
  
  
  RESULTS
  MEG3 decreased significantly in prostate cancer tissues relative to adjacent normal tissues. MEG3 inhibited intrinsic cell survival pathway in vitro and in vivo by reducing the protein expression of Bcl-2, enhancing Bax and activating caspase 3. We further demonstrated that MEG3 inhibited the expression of cell cycle regulatory protein Cyclin D1 and induced cell cycle arrest in G0/G1 phase.
  
  
  CONCLUSIONS
  Our study presents an important role of MEG3 in the molecular etiology of prostate cancer and implicates the potential application of MEG3 in prostate cancer therapy.
authors.forename: Gang
authors.initials: G
authors.lastname: Luo
authors.forename: Miao
authors.initials: M
authors.lastname: Wang
authors.forename: Xinchao
authors.initials: X
authors.lastname: Wu
authors.forename: Dan
authors.initials: D
authors.lastname: Tao
authors.forename: Xinyuan
authors.initials: X
authors.lastname: Xiao
authors.forename: Liang
authors.initials: L
authors.lastname: Wang
authors.forename: Fan
authors.initials: F
authors.lastname: Min
authors.forename: Fuqing
authors.initials: F
authors.lastname: Zeng
authors.forename: Guosong
authors.initials: G
authors.lastname: Jiang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D053148:Caspase 3 / Q000378:metabolism; D049109:Cell Proliferation / Q000235:genetics*; D019938:Cyclin D1 / Q000378:metabolism; D004789:Enzyme Activation; D064593:Heterografts; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D011471:Prostatic Neoplasms / Q000378:metabolism / Q000473:pathology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D062085:RNA, Long Noncoding / Q000502:physiology*
chemical_list: C498907:MEG3 non-coding RNA, human; D019253:Proto-Oncogene Proteins c-bcl-2; D062085:RNA, Long Noncoding; D019938:Cyclin D1; D053148:Caspase 3