title: Semisynthetic UbH2A reveals different activities of deubiquitinases and inhibitory effects of H2A K119 ubiquitination on H3K36 methylation in mononucleosomes.
abstract: Using a genetically incorporated azidonorleucine for ubiquitin installation, we prepared multi-milligram quantities of H2AK119ub (ubH2A). With a native isopeptide linkage, the synthetic ubH2A was used to study the activity of deubiquitinases and crosstalk between H2A ubiquitination and H3K36 methylation in the context of chemically defined mononucleosomes.
authors.affiliation: Division of Structural Biology and Biochemistry, School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore. cfliu@ntu.edu.sg.
authors.forename: Xiaobao
authors.initials: X
authors.lastname: Bi
authors.forename: Renliang
authors.initials: R
authors.lastname: Yang
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Feng
authors.forename: Daniela
authors.initials: D
authors.lastname: Rhodes
authors.forename: Chuan-Fa
authors.initials: CF
authors.lastname: Liu
mesh_terms: D000818:Animals; D001386:Azides / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D006657:Histones / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D008745:Methylation / Q000187:drug effects; D015394:Molecular Structure; D009646:Norleucine / Q000031:analogs & derivatives / Q000235:genetics / Q000378:metabolism; D009707:Nucleosomes / Q000187:drug effects* / Q000378:metabolism; D064570:Ubiquitin-Specific Proteases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D054875:Ubiquitination; D014452:Ubiquitins / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*; D014982:Xenopus laevis
chemical_list: D001386:Azides; D004791:Enzyme Inhibitors; D006657:Histones; D009707:Nucleosomes; D014452:Ubiquitins; C000609218:azidonorleucine; C021645:chromatin conjugate protein A24; D009646:Norleucine; D064570:Ubiquitin-Specific Proteases