title: Spot Matching of 2-DE Images Using Distance, Intensity, and Pattern Information.
abstract: The analysis of a large number of two-dimensional gel electrophoresis (2-DE) images requires developing automatic methods. In such analyses, spot matching plays a fundamental role, in particular for the identification of proteins. We describe a simple and accurate method which allows to automatically and accurately match spots in 2-DE images. The method consists of simultaneously exploiting the distance between the spots, their intensity, and the pattern formed by their spatial configuration.
authors.affiliation: College of Physics & Electronics, Shandong Normal University, 250014, Shandong, China.|CREATIS, CNRS UMR 5220, Inserm U1044, INSA Lyon, University of Lyon, Bâtiment Blaise Pascal, 69621, Villeurbanne cedex, France.
authors.forename: Hua-Mei
authors.initials: HM
authors.lastname: Xin
authors.affiliation: CREATIS, CNRS UMR 5220, Inserm U1044, INSA Lyon, University of Lyon, Bâtiment Blaise Pascal, 69621, Villeurbanne cedex, France. zhu@creatis.insa-lyon.fr.
authors.forename: Yuemin
authors.initials: Y
authors.lastname: Zhu
mesh_terms: D000465:Algorithms; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification*; D012984:Software
chemical_list: D011506:Proteins
keywords: 2-DE image s; Image registration; Proteomics; Spot matching; Spot registration; Two-dimensional gel electrophoresis