title: Transient Protein Expression by Agroinfiltration in Lettuce.
abstract: Current systems of recombinant protein production include bacterial, insect, and mammalian cell culture. However, these platforms are expensive to build and operate at commercial scales and/or have limited abilities to produce complex proteins. In recent years, plant-based expression systems have become top candidates for the production of recombinant proteins as they are highly scalable, robust, safe, and can produce complex proteins due to having a eukaryotic endomembrane system. Newly developed "deconstructed" viral vectors delivered via Agrobacterium tumefaciens (agroinfiltration) have enabled robust plant-based production of proteins with a wide range of applications. The leafy Lactuca sativa (lettuce) plant with its strong foundation in agriculture is an excellent host for pharmaceutical protein production. Here, we describe a method for agroinfiltration of lettuce that can rapidly produce high levels of recombinant proteins in a matter of days and has the potential to be scaled up to an agricultural level.
authors.affiliation: The Biodesign Institute, Arizona State University, Tempe, AZ, 85225, USA. qiang.chen.4@asu.edu.|School of Life Sciences, Arizona State University, Tempe, AZ, 85225, USA. qiang.chen.4@asu.edu.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Chen
authors.affiliation: The Biodesign Institute, Arizona State University, Tempe, AZ, 85225, USA.|School of Life Sciences, Arizona State University, Tempe, AZ, 85225, USA.
authors.forename: Matthew
authors.initials: M
authors.lastname: Dent
authors.affiliation: The Biodesign Institute, Arizona State University, Tempe, AZ, 85225, USA.|School of Life Sciences, Arizona State University, Tempe, AZ, 85225, USA.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Hurtado
authors.affiliation: The Biodesign Institute, Arizona State University, Tempe, AZ, 85225, USA.|School of Life Sciences, Arizona State University, Tempe, AZ, 85225, USA.
authors.forename: Jake
authors.initials: J
authors.lastname: Stahnke
authors.affiliation: The Biodesign Institute, Arizona State University, Tempe, AZ, 85225, USA.|School of Life Sciences, Arizona State University, Tempe, AZ, 85225, USA.
authors.forename: Alyssa
authors.initials: A
authors.lastname: McNulty
authors.affiliation: The Biodesign Institute, Arizona State University, Tempe, AZ, 85225, USA.
authors.forename: Kahlin
authors.initials: K
authors.lastname: Leuzinger
authors.affiliation: The Biodesign Institute, Arizona State University, Tempe, AZ, 85225, USA. Huafang.lai@asu.edu.
authors.forename: Huafang
authors.initials: H
authors.lastname: Lai
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics*; D015870:Gene Expression; D005818:Genetic Engineering / Q000379:methods*; D005822:Genetic Vectors; D018545:Lactuca / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D011994:Recombinant Proteins; D014170:Transformation, Genetic
chemical_list: D011994:Recombinant Proteins
keywords: Agrobacterium tumefaciens; Agroinfiltration; Antibodies; Biotechnology; Large-scale production; Lettuce; Plant biology; Plant-made pharmaceutics; Recombinant protein; Transient expression; Vaccines