title: Rapid identification of clinical members of Fusarium fujikuroi complex using MALDI-TOF MS.
abstract: OBJECTIVE
  To develop the matrix-assisted laser desorption ionization mass spectrometry (MALDI-TOF MS) method for identification of Fusarium species within Fusarium fujikuroi complex for use in clinical microbiology laboratories.
  
  
  METHODS
  A total of 24 reference and 60 clinical and environmental isolates belonging to the F. fujikuroi complex were subjected to MALDI-TOF MS identification. Protein extracts of Fusarium isolates were obtained using formic acid extraction. Multilocus sequence analysis was used as a gold standard.
  
  
  RESULTS
  The MALDI-TOF MS Biotyper correctly identified 93.6% of the strains down to the species level, while the remaining isolates (6.4%) were identified at the genus level when using scores of ≥ 2.0 as cut-off values. Correct identification was obtained despite large intraspecific heterogeneities in MALDI-TOF spectra.
  
  
  CONCLUSIONS
  MALDI-TOF MS is a rapid identification tool for the recognition of species within F. fujikuroi complex, provided a database is available.
authors.affiliation: CBS-KNAW Fungal Biodiversity Centre, Utrecht, The Netherlands.|Institute of Biodiversity & Ecosystem Dynamics, University of Amsterdam, Amsterdam, The Netherlands.|Directorate General of Health Services, Ministry of Health, Ibri Hospital, Ibri, Oman.
authors.forename: Abdullah M S
authors.initials: AM
authors.lastname: Al-Hatmi
authors.affiliation: Laboratoire de Parasitologie-Mycologie, CHU Timone, Université de la Méditerranée, Marseille, France.
authors.forename: Anne-Cécile
authors.initials: AC
authors.lastname: Normand
authors.affiliation: CBS-KNAW Fungal Biodiversity Centre, Utrecht, The Netherlands.
authors.forename: Anne D
authors.initials: AD
authors.lastname: van Diepeningen
authors.affiliation: BCCM/IHEM: Scientific Institute of Public Health, Mycology & Aerobiology Section, Bruxelles, Belgium.
authors.forename: Marijke
authors.initials: M
authors.lastname: Hendrickx
authors.affiliation: CBS-KNAW Fungal Biodiversity Centre, Utrecht, The Netherlands.|Institute of Biodiversity & Ecosystem Dynamics, University of Amsterdam, Amsterdam, The Netherlands.|Peking University Health Science Center, Research Center for Medical Mycology, Beijing, China.|Shanghai Institute of Medical Mycology, Changzheng Hospital, Second Military Medical University, Shanghai, China.|King Abdulaziz University, Jeddah, Saudi Arabia.
authors.forename: G Sybren
authors.initials: GS
authors.lastname: de Hoog
authors.affiliation: Laboratoire de Parasitologie-Mycologie, CHU Timone, Université de la Méditerranée, Marseille, France.
authors.forename: Renaud
authors.initials: R
authors.lastname: Piarroux
mesh_terms: D003955:Diagnostic Tests, Routine / Q000379:methods*; D060585:Fusariosis / Q000175:diagnosis* / Q000382:microbiology*; D005670:Fusarium / Q000737:chemistry* / Q000145:classification / Q000302:isolation & purification*; D006801:Humans; D008828:Microbiological Techniques / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013997:Time Factors
keywords: Fusarium fujikuroi complex; MALDI-TOF MS; database; fingerprint; multi-locus sequence analysis; spectral analysis