title: Characterization of a new Lactobacillus salivarius strain engineered to express IBV multi-epitope antigens by chromosomal integration.
abstract: To obtain adhesive and safe lactic acid bacteria (LAB) strains for expressing heterologous antigens, we screened LAB inhabitants in intestine of Tibetan chickens by analyzing their adhesion and safety properties and the selected LAB was engineered to express heterologous antigen (UTEpi C-A) based on chromosomal integration strategy. We demonstrated that a new Lactobacillu salivarius TCMM17 strain is strongly adhesive to chicken intestinal epithelial cells, contains no endogenous plasmids, is susceptible to tested antimicrobials, and shows no toxicities. In order to examine the potential of TCMM17 strain as heterogenous antigen delivering vehicle, we introduced a UTEpi C-A expression cassette in its chromosome by constructing a non-replicative plasmid (pORI280-UUTEpi C-AD). The recombinant TCMM17 strain (∆TCMM17) stably was found to keep the gene cassette through 50 generations, and successfully displayed EpiC encoded by the cassette on its surface. This work provides a universal platform for development of novel oral vaccines and expression of further antigens of avian pathogens.
authors.affiliation: a Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, ''985 Project'' Science Innovative Platform for Resource and Environment Protection of Southwestern, Key Laboratory of Bio-resources and Eco-environment , School of Life Science, Sichuan University, Ministry of Education , Chengdu , China.
authors.forename: Bing-cun
authors.initials: BC
authors.lastname: Ma
authors.affiliation: a Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, ''985 Project'' Science Innovative Platform for Resource and Environment Protection of Southwestern, Key Laboratory of Bio-resources and Eco-environment , School of Life Science, Sichuan University, Ministry of Education , Chengdu , China.
authors.forename: Xin
authors.initials: X
authors.lastname: Yang
authors.affiliation: a Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, ''985 Project'' Science Innovative Platform for Resource and Environment Protection of Southwestern, Key Laboratory of Bio-resources and Eco-environment , School of Life Science, Sichuan University, Ministry of Education , Chengdu , China.
authors.forename: Hong-ning
authors.initials: HN
authors.lastname: Wang
authors.affiliation: a Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, ''985 Project'' Science Innovative Platform for Resource and Environment Protection of Southwestern, Key Laboratory of Bio-resources and Eco-environment , School of Life Science, Sichuan University, Ministry of Education , Chengdu , China.
authors.forename: Hai-peng
authors.initials: HP
authors.lastname: Cao
authors.affiliation: a Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, ''985 Project'' Science Innovative Platform for Resource and Environment Protection of Southwestern, Key Laboratory of Bio-resources and Eco-environment , School of Life Science, Sichuan University, Ministry of Education , Chengdu , China.
authors.forename: Peng-wei
authors.initials: PW
authors.lastname: Xu
authors.affiliation: a Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, ''985 Project'' Science Innovative Platform for Resource and Environment Protection of Southwestern, Key Laboratory of Bio-resources and Eco-environment , School of Life Science, Sichuan University, Ministry of Education , Chengdu , China.
authors.forename: Meng-die
authors.initials: MD
authors.lastname: Ding
authors.affiliation: a Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, ''985 Project'' Science Innovative Platform for Resource and Environment Protection of Southwestern, Key Laboratory of Bio-resources and Eco-environment , School of Life Science, Sichuan University, Ministry of Education , Chengdu , China.
authors.forename: Hui
authors.initials: H
authors.lastname: Liu
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics*; D002645:Chickens; D002876:Chromosomes, Bacterial; D000939:Epitopes / Q000235:genetics*; D005260:Female; D007778:Lactobacillus / Q000235:genetics* / Q000276:immunology; D008297:Male
chemical_list: D000942:Antigens, Bacterial; D000939:Epitopes
keywords: Lactobacillus salivarius; Tibetan chicken; chromosomal integration; thyA-deficient