title: Paenibacillus etherisp. nov., able to grow on media supplemented with methyltert-butyl ether (MTBE) and isolated from hydrocarbon-contaminated soil.
abstract: A bacterial strain, designated SH7T, was isolated from the hydrocarbon-contaminated soil of a pilot plant (Granada, Spain). The strain was selected for its capacity to grow in media supplemented with methyltert-butyl ether (MTBE) as sole energy and carbon source. Strain SH7Twas a Gram-stain-positive, facultatively anaerobic, spore-forming, rod-shaped bacterium. Phylogenetic analysis using 16S rRNA gene sequences showed that strain SH7Tbelongs to a cluster comprising species of the genusPaenibacillusand was closely related toPaenibacillus borealisKK19T(97 % 16S rRNA gene sequence similarity) andPaenibacillus odoriferTOD45T(98 %). DNA-DNA hybridization tests showed low relatedness of strain SH7Twith the type strains ofPaenibacillus borealis(16.9 ± 1.5 %) andPaenibacillus odorifer(16.6 ± 2.1 %). The cell wall of strain SH7Tcontainedmeso-diaminopimelic acid. The predominant respiratory quinone was MK-7, and anteiso-C15 : 0 (32.9 %) and C16 : 0 (29.0 %) were the predominant cellular fatty acids. Phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol and three unknown aminophospholipids were the major phospholipids. The DNA G+C content was 44.3 mol%. Data obtained in this study indicate that strain SH7Trepresents a novel species of the genusPaenibacillus, for which the namePaenibacillus etherisp. nov. is proposed. The type strain is SH7T( = CECT 8558T= DSM 29760T).
authors.affiliation: Environmental Microbiology Group, Institute of Water Research, University of Granada, C/ Ramón y Cajal, n°4, 18071, Granada, Spain.|Department of Microbiology, Faculty of Sciences, University of Granada, Granada, Spain.
authors.forename: Isabel M
authors.initials: IM
authors.lastname: Guisado
authors.affiliation: Environmental Microbiology Group, Institute of Water Research, University of Granada, C/ Ramón y Cajal, n°4, 18071, Granada, Spain.|Department of Microbiology, Faculty of Sciences, University of Granada, Granada, Spain.
authors.forename: Jessica
authors.initials: J
authors.lastname: Purswani
authors.affiliation: Environmental Microbiology Group, Institute of Water Research, University of Granada, C/ Ramón y Cajal, n°4, 18071, Granada, Spain.|Department of Microbiology, Faculty of Sciences, University of Granada, Granada, Spain.
authors.forename: Jesús
authors.initials: J
authors.lastname: González-López
authors.affiliation: Department of Microbiology, Faculty of Sciences, University of Granada, Granada, Spain.|Environmental Microbiology Group, Institute of Water Research, University of Granada, C/ Ramón y Cajal, n°4, 18071, Granada, Spain.
authors.forename: Clementina
authors.initials: C
authors.lastname: Pozo