title: Assessment of chromosomal aberration in the bone marrow cells of Swiss Albino mice treated by 4-methylimidazole.
abstract: 4-Methylimidazole (4-MEI) is formed during the production of certain caramel coloring agents used in many food and drink products. It may also be formed during the cooking, roasting, or other processing of some foods and beverages. So it was unintentionally consumed in worldwide. This study was aimed to investigate the genotoxic and cytotoxic effects of 4-MEI using chromosome aberration (CA) and mitotic index (MI) in Swiss Albino mice. In this research, CA and MI of the mouse bone marrow cells were analyzed after treating the animals with 4-MEI (100, 130 and 160 mg/kg) for 12 h and 24 h treatment times. All data were analyzed using statistical methods. 4-MEI significantly increased the percentage of CAs at all concentrations for 12 h and at highest concentration for 24 h treatment periods. 4-MEI at highest concentration for 12 h and at all concentrations for 24 h decreased the MI in comparison with control. Genotoxic and cytotoxic effects of 4-MEI at 24 h treatment periods were concentration dependent. Consequently, it can be said that 4-MEI have genotoxic and cytotoxic effect in mouse.
authors.affiliation: a Department of Biotechnology , Institute of Basic and Applied Sciences, Cukurova University , Adana , Turkey .
authors.forename: Mostafa
authors.initials: M
authors.lastname: Norizadeh Tazehkand
authors.affiliation: b Faculty of Science and Letters , Department of Biology, Cukurova University , Adana , Turkey , and.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Topaktas
authors.affiliation: c Faculty of Medicine , Department of Medical Biology, Cukurova University , Adana , Turkey.
authors.forename: Mehmet Bertan
authors.initials: MB
authors.lastname: Yilmaz
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects* / Q000473:pathology; D002470:Cell Survival / Q000187:drug effects; D002869:Chromosome Aberrations / Q000139:chemically induced*; D004305:Dose-Response Relationship, Drug; D005260:Female; D007093:Imidazoles / Q000633:toxicity*; D008297:Male; D020714:Maximum Tolerated Dose; D051379:Mice; D008940:Mitotic Index
chemical_list: D007093:Imidazoles; C023439:4-methylimidazole
keywords: 4-Methylimidazole (4-MEI; CAS No: C-9754); chromosomal aberrations; mice bone marrow cells; mitotic index