title: Microbial Proteome Profiling and Systems Biology: Applications to Mycobacterium tuberculosis.
abstract: Each year, 1.3 million people die from tuberculosis, an infectious disease caused by Mycobacterium tuberculosis. Systems biology-based strategies might significantly contribute to the knowledge-guided development of more effective vaccines and drugs to prevent and cure infectious diseases. To build models simulating the behaviour of a system in response to internal or external stimuli and to identify potential targets for therapeutic intervention, systems biology approaches require the acquisition of quantitative molecular profiles on many perturbed states. Here we review the current state of proteomic analyses in Mycobacterium tuberculosis and discuss the potential of recently emerging targeting mass spectrometry-based techniques which enable fast, sensitive and accurate protein measurements.
authors.affiliation: Institute of Molecular Systems Biology, ETH Zurich, Zurich, CH-8093, Switzerland. schubert@imsb.biol.ethz.ch.|Systems Biology Graduate School, Zurich, CH-8057, Switzerland. schubert@imsb.biol.ethz.ch.
authors.forename: Olga T
authors.initials: OT
authors.lastname: Schubert
authors.affiliation: Institute of Molecular Systems Biology, ETH Zurich, Zurich, CH-8093, Switzerland. aebersold@imsb.biol.ethz.ch.|Faculty of Science, University of Zurich, Zurich, CH-8057, Switzerland. aebersold@imsb.biol.ethz.ch.
authors.forename: Ruedi
authors.initials: R
authors.lastname: Aebersold
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis* / Q000502:physiology; D009169:Mycobacterium tuberculosis / Q000737:chemistry*; D011499:Protein Processing, Post-Translational; D040901:Proteomics; D049490:Systems Biology / Q000379:methods*
chemical_list: D001426:Bacterial Proteins
keywords: Mass spectrometry; Mycobacterium tuberculosis; Proteome mapping; Proteomics; SWATH-MS; Selected reaction monitoring (SRM); Systems biology