title: Salt-stress-responsive chloroplast proteins in Brassica juncea genotypes with contrasting salt tolerance and their quantitative PCR analysis.
abstract: Brassica juncea is mainly cultivated in the arid and semi-arid regions of India where its production is significantly affected by soil salinity. Adequate knowledge of the mechanisms underlying the salt tolerance at sub-cellular levels must aid in developing the salt-tolerant plants. A proper functioning of chloroplasts under salinity conditions is highly desirable to maintain crop productivity. The adaptive molecular mechanisms offered by plants at the chloroplast level to cope with salinity stress must be a prime target in developing the salt-tolerant plants. In the present study, we have analyzed differential expression of chloroplast proteins in two Brassica juncea genotypes, Pusa Agrani (salt-sensitive) and CS-54 (salt-tolerant), under the effect of sodium chloride. The chloroplast proteins were isolated and resolved using 2DE, which facilitated identification and quantification of 12 proteins that differed in expression in the salt-tolerant and salt-sensitive genotypes. The identified proteins were related to a variety of chloroplast-associated molecular processes, including oxygen-evolving process, PS I and PS II functioning, Calvin cycle and redox homeostasis. Expression analysis of genes encoding differentially expressed proteins through real time PCR supported our findings with proteomic analysis. The study indicates that modulating the expression of chloroplast proteins associated with stabilization of photosystems and oxidative defence plays imperative roles in adaptation to salt stress.
authors.affiliation: Department of Botany, Molecular Ecology Laboratory, Jamia Hamdard, New Delhi, 110062, India.
authors.forename: Peerzada Yasir
authors.initials: PY
authors.lastname: Yousuf
authors.affiliation: Department of Botany, Aligarh Muslim University, Aligarh, 202002, India.
authors.forename: Altaf
authors.initials: A
authors.lastname: Ahmad
authors.affiliation: Department of Plant Production, College of Food and Agricultural Science, King Saud University, Post Box 2460, Riyadh, 11451, Saudi Arabia.
authors.forename: Ibrahim M
authors.initials: IM
authors.lastname: Aref
authors.affiliation: Department of Biology, Ege University, Izmir, 350000, Turkey.
authors.forename: Munir
authors.initials: M
authors.lastname: Ozturk
authors.affiliation: Department of Botany, Molecular Ecology Laboratory, Jamia Hamdard, New Delhi, 110062, India.
authors.lastname: Hemant
authors.affiliation: Department of Botany, Molecular Ecology Laboratory, Jamia Hamdard, New Delhi, 110062, India.
authors.forename: Arshid Hussain
authors.initials: AH
authors.lastname: Ganie
authors.affiliation: Department of Botany, Molecular Ecology Laboratory, Jamia Hamdard, New Delhi, 110062, India. iqbalg5@yahoo.co.in.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Iqbal
mesh_terms: D060365:Chloroplast Proteins / Q000378:metabolism*; D002736:Chloroplasts / Q000187:drug effects / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D005838:Genotype; D009149:Mustard Plant / Q000187:drug effects / Q000235:genetics* / Q000502:physiology*; D020543:Proteome / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D055051:Salt-Tolerant Plants / Q000187:drug effects / Q000235:genetics*; D012965:Sodium Chloride / Q000494:pharmacology*; D013312:Stress, Physiological / Q000187:drug effects*
chemical_list: D060365:Chloroplast Proteins; D020543:Proteome; D012965:Sodium Chloride
keywords: Brassica juncea; Chloroplast; Photosynthesis; Photosystems; Proteomics; Salt stress