title: Poly (ADP-ribose) synthetase inhibitor has a heart protective effect in a rat model of experimental sepsis.
abstract: The aim of this study is to investigate whether PARP inhibitor could reduce cell apoptosis and injury in the heart during sepsis.
  
  
  METHODS
  60 healthy male Sprague-Dawley (SD) rats were randomly divided into 4 groups---sham group, modal group, 3-AB pretreatment group and 3-AB treatment group, 15 rats per group. The cecal ligation and puncture (CLP) model of sepsis was used. The following were determined--levels of malondialdehyde (MDA), ATP and nicotinamide adenine dinucleotide (NAD+), expression of PARP, Bcl-2, Bax, cytochrome C and caspase 3 activity in the myocardium tissue, levels of serum creatine kinase muscle brain (CK-MB) fraction and troponin I.
  
  
  RESULTS
  Histological and molecular analyses showed that myocardial cells apoptosis were associated with mitochondria injury, with an increase in the amount of PARP and a decrease in ATP and NAD+ levels in model group. In addition, the levels of Bax, cytochrome C and caspase 3 activity, serum levels of CK-MB and troponin I increased, but levels of Bcl-2 significantly decreased. Inhibition of PARP upregulated the levels of ATP, NAD + and Bcl-2, and significantly reduced the activation of PARP and caspase 3, decreased the levels of MDA, cytochrome C, CK-MB and troponin I. As a result, apoptosis in the heart was attenuated.
  
  
  CONCLUSIONS
  These results indicate that PARP activation may be involved in apoptosis in the heart induced by sepsis and 3-AB could improve it.
authors.affiliation: Department of Histology and Embryology, Binzhou Medical College Yantai, P. R. China.
authors.forename: Lianshuang
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Cardiovascular Center, Hospital of Yantai Economic Technology Development Area Yantai, P. R. China.
authors.forename: Jinpeng
authors.initials: J
authors.lastname: Yao
authors.affiliation: Department of Critical Care Medicine, Yu Huang Ding Hospital, Qingdao University Yantai, P. R. China.
authors.forename: Xifeng
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Histology and Embryology, Binzhou Medical College Yantai, P. R. China.
authors.forename: Hongxing
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Histology and Embryology, Binzhou Medical College Yantai, P. R. China.
authors.forename: Tongshen
authors.initials: T
authors.lastname: Liu
authors.affiliation: Department of Histology and Embryology, Binzhou Medical College Yantai, P. R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhao
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D001549:Benzamides / Q000494:pharmacology; D004195:Disease Models, Animal; D006321:Heart / Q000187:drug effects*; D007150:Immunohistochemistry; D008297:Male; D046529:Microscopy, Electron, Transmission; D009206:Myocardium / Q000473:pathology; D018384:Oxidative Stress / Q000187:drug effects; D000067856:Poly(ADP-ribose) Polymerase Inhibitors / Q000494:pharmacology*; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D018805:Sepsis / Q000378:metabolism*
chemical_list: D001549:Benzamides; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; C025160:3-aminobenzamide; D011065:Poly(ADP-ribose) Polymerases
keywords: 3-AB; Sepsis; heart; rats