title: [A New Simple Technique for Producing Labeled Monoclonal Antibodies for Antibody Pair Screening in Sandwich-ELISA].
abstract: A simple and fast method for obtaining biotin-labeled monoclonal antibodies was developed usingcontent of hybridoma culture supernatant sufficient to select antibody pairs in sandwich ELISA. The method consists in chemical biotinylation of antigen-bound antibodies in a well of ELISA plate. Using as an example target Vaccinia virus A27L protein it was shown that the yield of biotinylated reactant is enough to set comprehensive sandwich ELISA for a moderate size panel of up to 25 monoclonal antibodies with an aim to determine candidate pairs. The technique is a cheap and effective solution since it avoids obtaining preparative amounts of antibodies.
authors.forename: M M
authors.initials: MM
authors.lastname: Zaripov
authors.forename: G V
authors.initials: GV
authors.lastname: Afanasieva
authors.forename: X A
authors.initials: XA
authors.lastname: Glukhova
authors.forename: Y A
authors.initials: YA
authors.lastname: Trizna
authors.forename: A S
authors.initials: AS
authors.lastname: Glukhov
authors.forename: I P
authors.initials: IP
authors.lastname: Beletsky
authors.forename: O V
authors.initials: OV
authors.lastname: Prusakova
mesh_terms: D058846:Antibodies, Monoclonal, Murine-Derived / Q000737:chemistry*; D000914:Antibodies, Viral / Q000737:chemistry*; D001710:Biotin / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D014616:Vaccinia virus; D014764:Viral Proteins / Q000032:analysis*
chemical_list: C110360:A27L protein, Orthopoxvirus; D058846:Antibodies, Monoclonal, Murine-Derived; D000914:Antibodies, Viral; D014764:Viral Proteins; D001710:Biotin