title: RNA-Seq and Mass-Spectrometry-Based Lipidomics Reveal Extensive Changes of Glycerolipid Pathways in Brown Adipose Tissue in Response to Cold.
abstract: Cold exposure greatly alters brown adipose tissue (BAT) gene expression and metabolism to increase thermogenic capacity. Here, we used RNA sequencing and mass-spectrometry-based lipidomics to provide a comprehensive resource describing the molecular signature of cold adaptation at the level of the transcriptome and lipidome. We show that short-term (3-day) cold exposure leads to a robust increase in expression of several brown adipocyte genes related to thermogenesis as well as the gene encoding the hormone irisin. However, pathway analysis shows that the most significantly induced genes are those involved in glycerophospholipid synthesis and fatty acid elongation. This is accompanied by significant changes in the acyl chain composition of triacylglycerols (TAGs) as well as subspecies-selective changes of acyl chains in glycerophospholipids. These results indicate that cold adaptation of BAT is associated with significant and highly species-selective remodeling of both TAGs and glycerophospholipids.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Southern Denmark, 5230 Odense M, Denmark.
authors.forename: Ann-Britt
authors.initials: AB
authors.lastname: Marcher
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Southern Denmark, 5230 Odense M, Denmark.
authors.forename: Anne
authors.initials: A
authors.lastname: Loft
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Southern Denmark, 5230 Odense M, Denmark.
authors.forename: Ronni
authors.initials: R
authors.lastname: Nielsen
authors.affiliation: Zora Biosciences Oy, Biologinkuja 1, 02150 Espoo, Finland.
authors.forename: Terhi
authors.initials: T
authors.lastname: Vihervaara
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Southern Denmark, 5230 Odense M, Denmark; Novo Nordisk Foundation Center of Basic Metabolic Research, University of Copenhagen, 2200 Copenhagen, Denmark.
authors.forename: Jesper Grud Skat
authors.initials: JG
authors.lastname: Madsen
authors.affiliation: Zora Biosciences Oy, Biologinkuja 1, 02150 Espoo, Finland.
authors.forename: Marko
authors.initials: M
authors.lastname: Sysi-Aho
authors.affiliation: Zora Biosciences Oy, Biologinkuja 1, 02150 Espoo, Finland.
authors.forename: Kim
authors.initials: K
authors.lastname: Ekroos
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Southern Denmark, 5230 Odense M, Denmark. Electronic address: s.mandrup@bmb.sdu.dk.
authors.forename: Susanne
authors.initials: S
authors.lastname: Mandrup
mesh_terms: D002001:Adipose Tissue, Brown / Q000378:metabolism*; D000818:Animals; D016000:Cluster Analysis; D003080:Cold Temperature; D005353:Fibronectins / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D020404:Glycerophospholipids / Q000378:metabolism*; D008297:Male; D013058:Mass Spectrometry; D051379:Mice; D008810:Mice, Inbred C57BL; D012313:RNA / Q000378:metabolism; D017423:Sequence Analysis, RNA; D022722:Thermogenesis; D013997:Time Factors; D059467:Transcriptome
chemical_list: C571428:FNDC5 protein, mouse; D005353:Fibronectins; D020404:Glycerophospholipids; D012313:RNA; D005978:Glutathione