title: Somatic Embryogenesis of Lilium from Microbulb Transverse Thin Cell Layers.
abstract: A reliable somatic embryogenesis protocol is a prerequisite for application of other plant biotechniques. Several protocols were reported for genus Lilium, with variable success. Between them, transverse Thin Cell Layers (tTCL) were used efficiently to induce indirect somatic embryogenesis of Lilium. Somatic embryogenesis potential is dependent on the genotype, explant, and culture medium composition, especially as for plant growth regulators and environmental conditions. Usually, the process comprises three phases: embryogenic callus induction, embryogenic callus proliferation and somatic embryo germination. Somatic embryo germination can be achieved in light or dark. In the first case, complete plantlets are formed, with green leaves and pseudobulb in the base. In darkness, microbulbs are formed from single somatic embryos or clusters. A last phase of microbulb enlargement allows plantlets or microbulbs to increase their biomass. These enlarged microbulbs do not need special acclimatization conditions when transferred to soil and quickly produce sturdy plants. This chapter describes a protocol for somatic embryogenesis of Lilium using tTCL from microbulbs.
authors.affiliation: Agronomy Department, Universidad Nacional del Sur, Centro de Recursos Naturales Renovables de la Zona Semiárida (CERZOS), National Research Council (CONICET), Bahia Blanca, Buenos Aires, 8000, Argentina. pamarina@cerzos-conicet.gob.ar.
authors.forename: Pablo
authors.initials: P
authors.lastname: Marinangeli
mesh_terms: D000064:Acclimatization / Q000235:genetics; D018525:Germination / Q000235:genetics; D027762:Lilium / Q000235:genetics / Q000254:growth & development*; D063245:Plant Development / Q000235:genetics; D010937:Plant Growth Regulators / Q000378:metabolism; D018515:Plant Leaves / Q000235:genetics / Q000254:growth & development; D018517:Plant Roots / Q000235:genetics / Q000254:growth & development*; D018520:Plant Shoots / Q000235:genetics / Q000254:growth & development; D058439:Plant Somatic Embryogenesis Techniques / Q000379:methods*; D030821:Plants, Genetically Modified / Q000235:genetics; D046509:Tissue Culture Techniques / Q000379:methods*
chemical_list: D010937:Plant Growth Regulators
keywords: Lilium; Plant tissue culture; Somatic embryo s; Transverse thin cell layer s; tTCL