title: Effect of adipose-derived stem cell-conditioned medium on the proliferation and migration of B16 melanoma cells.
abstract: Adipose-derived stem cells (ASCs) are a population of cells derived from adipose tissue. ASCs exhibit multilineage development potential and are able to secrete various factors, which influence adjacent cells. Previous studies have reported the effectiveness of ASC-conditioned medium (ASC-CM) in wound healing, anti-melanogenesis, wrinkle improvement and hair growth. In the present study, the anticancer function of ASC-CM was investigatedin vitroandin vivo. An MTT assay revealed that ASC-CM significantly decreased the proliferation of B16 melanoma cells in a time- and dose-dependent manner (P<0.01). Cell cycle analysis indicated that ASC-CM significantly increased the number of cells in G1 phase while reducing the number of cells in the S and G2/M phases (P<0.01). Furthermore, a wound migration model demonstrated that ASC-CM treatment significantly decreased the migration ability of B16 melanoma cells (P<0.01). In addition, C57BL/6 mice were administered with a single intratumoral injection of ASC-CM, daily or every other day, and a significant reduction in the volume of the tumor mass was observed compared with that of the control group (P<0.01). Thus, the findings of the present study indicated that ASC-CM has an anti-tumorigenic effect on B16 melanoma cellsin vitroandin vivo, and may potentially be used to support the treatment of melanoma in the future.
authors.affiliation: Department of Biomedical Sciences, College of Medicine, Inha University, Incheon 400-712, Republic of Korea.
authors.forename: Ju-Hee
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of Biomedical Sciences, College of Medicine, Inha University, Incheon 400-712, Republic of Korea.
authors.forename: Chul Hong
authors.initials: CH
authors.lastname: Park
authors.affiliation: Gachon Institute of Pharmaceutical Sciences, College of Pharmacy, Gachon University, Incheon 406-799, Republic of Korea.
authors.forename: Kwang-Hoon
authors.initials: KH
authors.lastname: Chun
authors.affiliation: Department of Biomedical Sciences, College of Medicine, Inha University, Incheon 400-712, Republic of Korea.
authors.forename: Soon-Sun
authors.initials: SS
authors.lastname: Hong
keywords: B16 melanoma cells; adipose-derived stem cell-conditioned media; adipose-derived stem cells; melanoma