title: In vitro and in vivo anti-melanoma effects of Daphne gnidium aqueous extract via activation of the immune system.
abstract: The purpose of this study was to assess the antitumor and immunomodulatory effects of the aqueous extract from Daphne gnidium in mice-bearing melanoma tumor. Balb/C mice were subcutaneously implanted with B16-F10 cells and treated intraperitoneally with the aqueous extract at 200 mg/Kg b.w for 21 days. After euthanization on day 22, the tumors were weighed; lymphocyte proliferation, cytotoxic T lymphocyte (CTL), and natural killer (NK) cell activities were evaluated using the MTT assay. Macrophage phagocytosis was studied by measuring the lysosomal activity. In addition to its potential to inhibit the growth of the transplantable tumor, the aqueous extract remarkably induced splenocyte proliferation and both NK and CTL activities in tumor-bearing mice. The aqueous extract was also seen to have promoted lysosomal activity of host macrophages.
authors.affiliation: Unité de substances naturelles bioactives et biotechnology, Faculté de pharmacie de Monastir, Rue Avicenne, 5000, Monastir, Tunisia.
authors.forename: Fadwa
authors.initials: F
authors.lastname: Chaabane
authors.affiliation: Unité de substances naturelles bioactives et biotechnology, Faculté de pharmacie de Monastir, Rue Avicenne, 5000, Monastir, Tunisia.
authors.forename: Nadia
authors.initials: N
authors.lastname: Mustapha
authors.affiliation: Unité de substances naturelles bioactives et biotechnology, Faculté de pharmacie de Monastir, Rue Avicenne, 5000, Monastir, Tunisia.
authors.forename: Imen
authors.initials: I
authors.lastname: Mokdad-Bzeouich
authors.affiliation: Unité de substances naturelles bioactives et biotechnology, Faculté de pharmacie de Monastir, Rue Avicenne, 5000, Monastir, Tunisia.
authors.forename: Aicha
authors.initials: A
authors.lastname: Sassi
authors.affiliation: Unité de substances naturelles bioactives et biotechnology, Faculté de pharmacie de Monastir, Rue Avicenne, 5000, Monastir, Tunisia.
authors.forename: Soumaya
authors.initials: S
authors.lastname: Kilani-Jaziri
authors.affiliation: UMR 5557 CNRS/Université Lyon1, Equipe Multirésistance environnementale et efflux bactérien, Centre d'étude des substances naturelles, Lyon, France.
authors.forename: Marie-Geneviève
authors.initials: MG
authors.lastname: Dijoux Franca
authors.affiliation: UMR 5557 CNRS/Université Lyon1, Equipe Multirésistance environnementale et efflux bactérien, Centre d'étude des substances naturelles, Lyon, France.
authors.forename: Serge
authors.initials: S
authors.lastname: Michalet
authors.affiliation: Unité de substances naturelles bioactives et biotechnology, Faculté de pharmacie de Monastir, Rue Avicenne, 5000, Monastir, Tunisia.
authors.forename: Mayssa
authors.initials: M
authors.lastname: Fathallah
authors.affiliation: Unité de substances naturelles bioactives et biotechnology, Faculté de pharmacie de Monastir, Rue Avicenne, 5000, Monastir, Tunisia. mounira.krifa@laposte.net.
authors.forename: Mounira
authors.initials: M
authors.lastname: Krifa
authors.affiliation: Unité de substances naturelles bioactives et biotechnology, Faculté de pharmacie de Monastir, Rue Avicenne, 5000, Monastir, Tunisia.
authors.forename: Kamel
authors.initials: K
authors.lastname: Ghedira
authors.affiliation: Unité de substances naturelles bioactives et biotechnology, Faculté de pharmacie de Monastir, Rue Avicenne, 5000, Monastir, Tunisia.|Laboratoire de biologie cellulaire et moléculaire, Faculté de médecine dentaire de Monastir, Rue Avicenne, 5000, Monastir, Tunisia.
authors.forename: Leila
authors.initials: L
authors.lastname: Chekir-Ghedira
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D032364:Daphne / Q000737:chemistry*; D004195:Disease Models, Animal; D007107:Immune System / Q000187:drug effects*; D007155:Immunologic Factors / Q000494:pharmacology*; D015603:Keratinocytes / Q000187:drug effects / Q000378:metabolism; D007694:Killer Cells, Natural / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D008247:Lysosomes / Q000378:metabolism; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D008546:Melanoma, Experimental / Q000188:drug therapy / Q000276:immunology / Q000473:pathology; D051379:Mice; D010936:Plant Extracts / Q000494:pharmacology*; D013602:T-Lymphocytes, Cytotoxic / Q000187:drug effects / Q000276:immunology / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D007155:Immunologic Factors; D010936:Plant Extracts
keywords: Anti-melanoma; B16-F10 cells; Daphne gnidium; Immunomodulation; Keratinocytes