title: Mitochondrial DNA deletions in patients with chronic suppurative otitis media.
abstract: The aim of this study was to investigate the 4977 and 7400 bp deletions of mitochondrial DNA in patients with chronic suppurative otitis media and to indicate the possible association of mitochondrial DNA deletions with chronic suppurative otitis media. Thirty-six patients with chronic suppurative otitis media were randomly selected to assess the mitochondrial DNA deletions. Tympanomastoidectomy was applied for the treatment of chronic suppurative otitis media, and the curettage materials including middle ear tissues were collected. The 4977 and 7400 bp deletion regions and two control regions of mitochondrial DNA were assessed by using the four pair primers. DNA was extracted from middle ear tissues and peripheral blood samples of the patients, and then polymerase chain reactions (PCRs) were performed. PCR products were separated in 2 % agarose gel. Seventeen of 36 patients had the heterozygote 4977 bp deletion in the middle ear tissue but not in peripheral blood. There wasn't any patient who had the 7400 bp deletion in mtDNA of their middle ear tissue or peripheral blood tissue. The patients with the 4977 bp deletion had a longer duration of chronic suppurative otitis media and a higher level of hearing loss than the others (p < 0.01). Long time chronic suppurative otitis media and the reactive oxygen species can cause the mitochondrial DNA deletions and this may be a predisposing factor to sensorineural hearing loss in chronic suppurative otitis media. An antioxidant drug as a scavenger agent may be used in long-term chronic suppurative otitis media.
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, Faculty of Medicine, Ataturk University, Erzurum, Turkey. arzutatar@atauni.edu.tr.
authors.forename: Arzu
authors.initials: A
authors.lastname: Tatar
authors.affiliation: Department of Medical Genetics, Faculty of Medicine, Ataturk University, Erzurum, Turkey.
authors.forename: Sener
authors.initials: S
authors.lastname: Tasdemir
authors.affiliation: Department of Medical Genetics, Faculty of Medicine, Ataturk University, Erzurum, Turkey.
authors.forename: Ibrahim
authors.initials: I
authors.lastname: Sahin
authors.affiliation: Department of Molecular Biology, Faculty of Science, Ataturk University, Erzurum, Turkey.
authors.forename: Ceyda
authors.initials: C
authors.lastname: Bozoglu
authors.affiliation: Department of Medical Genetics, Faculty of Medicine, Ataturk University, Erzurum, Turkey.
authors.forename: Haktan Bagis
authors.initials: HB
authors.lastname: Erdem
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, Faculty of Medicine, Ataturk University, Erzurum, Turkey.
authors.forename: Ozgur
authors.initials: O
authors.lastname: Yoruk
authors.affiliation: Department of Medical Genetics, Faculty of Medicine, Ataturk University, Erzurum, Turkey.
authors.forename: Abdulgani
authors.initials: A
authors.lastname: Tatar
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002908:Chronic Disease; D004272:DNA, Mitochondrial / Q000235:genetics*; D005260:Female; D017353:Gene Deletion; D006319:Hearing Loss, Sensorineural / Q000209:etiology; D006579:Heterozygote; D006801:Humans; D008297:Male; D008875:Middle Aged; D010035:Otitis Media, Suppurative / Q000235:genetics*; D016133:Polymerase Chain Reaction; D055815:Young Adult
chemical_list: D004272:DNA, Mitochondrial
keywords: 4977 bp deletion; 7400 bp deletion; Chronic otitis media; Hearing loss; mtDNA