title: Erinacine C: A novel approach to produce the secondary metabolite by submerged cultivation of Hericium erinaceus.
abstract: Erinacine C is a cyathane scaffold-based secondary metabolite, which is naturally produced by the filamentous fungus Hericium erinaceus and has a high potential to treat nervous diseases such as Alzheimer's disease. The investigated approach consists of combining an optimised precultivation of H. erinaceus with an enhanced erinacine C production by developing a suitable main cultivation medium enabling the utilisation of high biomass contents. The final erinacine C production medium is buffered by 100 mM HEPES to ensure a stable pH value of 7.5 during main cultivation at inoculation ratios of up to 5:10 (v/v). The medium components, such as 5.0 g L(-1) oatmeal, 1.5 g L(-1) calcium carbonate, and 0.5 g L(-1) Edamin(®) K are crucial for an increased erinacine C production. Besides, different carbon to nitrogen ratios of 25, 64, and 103 do not affect the erinacine C synthesis. The investigated approach enables the production of 2.73 g erinacine C per litre main cultivation broth, which is tenfold higher than published data. In addition, erinacine C biosynthesis is determined to occur mainly in the first six days of main cultivation.
authors.affiliation: Department of Biochemical and Chemical Engineering, TU Dortmund University, Emil-Figge-Straße 70, 44227 Dortmund, Germany.
authors.forename: Niklas
authors.initials: N
authors.lastname: Wolters
authors.affiliation: Department of Biochemical and Chemical Engineering, TU Dortmund University, Emil-Figge-Straße 70, 44227 Dortmund, Germany.
authors.forename: Gerhard
authors.initials: G
authors.lastname: Schembecker
authors.affiliation: Department of Biochemical and Chemical Engineering, TU Dortmund University, Emil-Figge-Straße 70, 44227 Dortmund, Germany. Electronic address: Juliane.Merz@bci.tu-dortmund.de.
authors.forename: Juliane
authors.initials: J
authors.lastname: Merz
mesh_terms: D001487:Basidiomycota / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D018533:Biomass; D003470:Culture Media / Q000378:metabolism*; D004224:Diterpenes / Q000378:metabolism*; D007218:Industrial Microbiology / Q000379:methods*; D025282:Mycelium / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism; D064210:Secondary Metabolism
chemical_list: D003470:Culture Media; D004224:Diterpenes; C000610439:erinacine C; D009584:Nitrogen
keywords: Alzheimer's disease; Filamentous fungus; Functional food; Submerged fungus cultivation