title: AAV-Mediated Gene Transfer to Dorsal Root Ganglion.
abstract: Transferring genetic molecules into the peripheral sensory nervous system to manipulate nociceptive pathophysiology is a powerful approach for experimental modulation of sensory signaling and potentially for translation into therapy for chronic pain. This can be efficiently achieved by the use of recombinant adeno-associated virus (rAAV) in conjunction with nociceptor-specific regulatory transgene cassettes. Among different routes of delivery, direct injection into the dorsal root ganglia (DRGs) offers the most efficient AAV-mediated gene transfer selectively into the peripheral sensory nervous system. Here, we briefly discuss the advantages and applications of intraganglionic microinjection, and then provide a detailed approach for DRG injection, including a list of the necessary materials and description of a method for performing DRG microinjection experiments. We also discuss our experience with several adeno-associated virus (AAV) options for in vivo transgene expression in DRG neurons.
authors.affiliation: Department of Anesthesiology, Medical College of Wisconsin, Watertown Plank Road, Milwaukee, WI, 53226, USA. hyu@mcw.edu.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Yu
authors.affiliation: Department of Anesthesiology, Medical College of Wisconsin, Watertown Plank Road, Milwaukee, WI, 53226, USA.
authors.forename: Gregory
authors.initials: G
authors.lastname: Fischer
authors.affiliation: Department of Anesthesiology, Medical College of Wisconsin, Watertown Plank Road, Milwaukee, WI, 53226, USA.
authors.forename: Quinn H
authors.initials: QH
authors.lastname: Hogan
mesh_terms: D000818:Animals; D059350:Chronic Pain / Q000628:therapy; D000229:Dependovirus / Q000235:genetics* / Q000378:metabolism; D005727:Ganglia, Spinal / Q000378:metabolism*; D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D005822:Genetic Vectors / Q000008:administration & dosage*; D008297:Male; D008845:Microinjections; D051381:Rats; D017207:Rats, Sprague-Dawley; D014161:Transduction, Genetic
keywords: Adeno-associated virus; Chronic pain; Dorsal root ganglion; Gene therapy; Microinjection