title: Targeted gold-coated iron oxide nanoparticles for CD163 detection in atherosclerosis by MRI.
abstract: CD163 is a membrane receptor expressed by macrophage lineage. Studies performed in atherosclerosis have shown that CD163 expression is increased at inflammatory sites, pointing at the presence of intraplaque hemorrhagic sites or asymptomatic plaques. Hence, imaging of CD163 expressing macrophages is an interesting strategy in order to detect atherosclerotic plaques. We have prepared a targeted probe based on gold-coated iron oxide nanoparticles vectorized with an anti-CD163 antibody for the specific detection of CD163 by MRI. Firstly, the specificity of the targeted probe was validated in vitro by incubation of the probe with CD163(+) or (-) macrophages. The probe was able to selectively detect CD163(+) macrophages both in human and murine cells. Subsequently, the targeted probe was injected in 16 weeks old apoE deficient mice developing atherosclerotic lesions and the pararenal abdominal aorta was imaged by MRI. The accumulation of probe in the site of interest increased over time and the signal intensity decreased significantly 48 hours after the injection. Hence, we have developed a highly sensitive targeted probe capable of detecting CD163-expressing macrophages that could provide useful information about the state of the atheromatous lesions.
authors.affiliation: Laboratorio de Patología Vascular y Renal. IIS Fundación Jiménez Díaz, Universidad Autónoma. Av. Reyes Católicos 2, 28040, Madrid, Spain.
authors.forename: Carlos
authors.initials: C
authors.lastname: Tarin
authors.affiliation: Laboratorio de Gliconanotecnología. Biofunctional Nanomaterials Unit. CIC biomaGUNE. Paseo Miramón, 182, 20009, San Sebastián, Spain.|Ikerbasque, Basque Foundation for Science, 48011, Bilbao, Spain.
authors.forename: Monica
authors.initials: M
authors.lastname: Carril
authors.affiliation: Laboratorio de Patología Vascular y Renal. IIS Fundación Jiménez Díaz, Universidad Autónoma. Av. Reyes Católicos 2, 28040, Madrid, Spain.
authors.forename: Jose Luis
authors.initials: JL
authors.lastname: Martin-Ventura
authors.affiliation: Molecular Imaging Unit, CIC biomaGUNE, PaseoMiramón 182, 20009, San Sebastián, Spain.
authors.forename: Irati
authors.initials: I
authors.lastname: Markuerkiaga
authors.affiliation: Molecular Imaging Unit, CIC biomaGUNE, PaseoMiramón 182, 20009, San Sebastián, Spain.
authors.forename: Daniel
authors.initials: D
authors.lastname: Padro
authors.affiliation: Laboratorio de Patología Vascular y Renal. IIS Fundación Jiménez Díaz, Universidad Autónoma. Av. Reyes Católicos 2, 28040, Madrid, Spain.
authors.forename: Patricia
authors.initials: P
authors.lastname: Llamas-Granda
authors.affiliation: Laboratorio de Patología Vascular y Renal. IIS Fundación Jiménez Díaz, Universidad Autónoma. Av. Reyes Católicos 2, 28040, Madrid, Spain.
authors.forename: Juan Antonio
authors.initials: JA
authors.lastname: Moreno
authors.affiliation: Laboratorio de Gliconanotecnología. Biofunctional Nanomaterials Unit. CIC biomaGUNE. Paseo Miramón, 182, 20009, San Sebastián, Spain.|Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Paseo Miramón 182, 20009, San Sebastián, Spain.
authors.forename: Isabel
authors.initials: I
authors.lastname: García
authors.affiliation: Laboratorio de Gliconanotecnología. Biofunctional Nanomaterials Unit. CIC biomaGUNE. Paseo Miramón, 182, 20009, San Sebastián, Spain.
authors.forename: Nuria
authors.initials: N
authors.lastname: Genicio
authors.affiliation: Molecular Imaging Unit, CIC biomaGUNE, PaseoMiramón 182, 20009, San Sebastián, Spain.
authors.forename: Sandra
authors.initials: S
authors.lastname: Plaza-Garcia
authors.affiliation: Laboratorio de Patología Vascular y Renal. IIS Fundación Jiménez Díaz, Universidad Autónoma. Av. Reyes Católicos 2, 28040, Madrid, Spain.
authors.forename: Luis Miguel
authors.initials: LM
authors.lastname: Blanco-Colio
authors.affiliation: Laboratorio de Gliconanotecnología. Biofunctional Nanomaterials Unit. CIC biomaGUNE. Paseo Miramón, 182, 20009, San Sebastián, Spain.|Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Paseo Miramón 182, 20009, San Sebastián, Spain.
authors.forename: Soledad
authors.initials: S
authors.lastname: Penades
authors.affiliation: Laboratorio de Patología Vascular y Renal. IIS Fundación Jiménez Díaz, Universidad Autónoma. Av. Reyes Católicos 2, 28040, Madrid, Spain.
authors.forename: Jesus
authors.initials: J
authors.lastname: Egido
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism*; D015214:Antigens, Differentiation, Myelomonocytic / Q000378:metabolism*; D050197:Atherosclerosis / Q000175:diagnosis* / Q000378:metabolism* / Q000473:pathology; D002460:Cell Line; D004195:Disease Models, Animal; D005296:Ferrous Compounds / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D008264:Macrophages / Q000378:metabolism; D008279:Magnetic Resonance Imaging / Q000379:methods*; D058185:Magnetite Nanoparticles / Q000737:chemistry / Q000633:toxicity / Q000648:ultrastructure; D051379:Mice; D018345:Mice, Knockout; D058226:Plaque, Atherosclerotic / Q000175:diagnosis / Q000378:metabolism / Q000473:pathology; D011956:Receptors, Cell Surface / Q000378:metabolism*; D015203:Reproducibility of Results; D000098902:CD163 Antigen
chemical_list: D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D000098902:CD163 Antigen; D005296:Ferrous Compounds; D058185:Magnetite Nanoparticles; D011956:Receptors, Cell Surface; D006046:Gold; C034236:ferrous oxide