title: Microarray Expression Analysis for the Paradoxical Roles of Acanthopanax senticosus Harms in Treating α-Synucleinopathies.
abstract: α-Synuclein is a key player in the pathogenesis of neurodegenerative disorders with Lewy bodies. Our previous studies have also showed that Acanthopanax senticosus harms (AS) could significantly suppress α-synuclein overexpression and toxicity. Identifying the RNAs related to α-synucleinopathies may facilitate understanding the pathogenesis of the diseases and the safe application of AS in the clinic. Microarray expression profiling of long non-coding RNAs (lncRNAs) and mRNAs was undertaken in control non-transgenic and human α-synuclein transgenic mice. The effects of AS on central nervous system (CNS) in pathology and physiology were investigated based on the lncRNA/mRNA targets analysis. In total, 341 lncRNAs and 279 mRNAs were differentially expressed by α-synuclein stimulus, among which 29 lncRNAs and 25 mRNAs were involved in the anti-α-synucleinopathies mechanism of AS. However, the levels of 19/29 lncRNAs and 12/25 mRNAs in AS group were similar to those in α-synuclein group, which may cause potential neurotoxicity analogous to α-synuclein. This study demonstrated that some of lncRNAs/mRNAs were involved in α-synuclein related pathophysiology, and AS produced the bidirectional effects on CNS under pathological and physiological conditions.
authors.affiliation: Chinese Medicine Toxicological Laboratory, Heilongjiang University of Chinese Medicine, Harbin, 150040, China.|Department of Pharmacy, GuiYang College of Traditional Chinese Medicine, GuiYang, 550025, China.
authors.forename: Xu-zhao
authors.initials: XZ
authors.lastname: Li
authors.affiliation: Chinese Medicine Toxicological Laboratory, Heilongjiang University of Chinese Medicine, Harbin, 150040, China.
authors.forename: Shuai-nan
authors.initials: SN
authors.lastname: Zhang
authors.affiliation: Chinese Medicine Toxicological Laboratory, Heilongjiang University of Chinese Medicine, Harbin, 150040, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Lu
authors.affiliation: Chinese Medicine Toxicological Laboratory, Heilongjiang University of Chinese Medicine, Harbin, 150040, China.|Drug Safety Evaluation Center, Heilongjiang University of Chinese Medicine, Harbin, 150040, China.
authors.forename: Shu-min
authors.initials: SM
authors.lastname: Liu
mesh_terms: D000818:Animals; D028001:Eleutherococcus / Q000009:adverse effects / Q000737:chemistry*; D006801:Humans; D016631:Lewy Bodies; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D020411:Oligonucleotide Array Sequence Analysis; D010936:Plant Extracts / Q000494:pharmacology*; D062085:RNA, Long Noncoding / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D059467:Transcriptome; D051844:alpha-Synuclein / Q000235:genetics*
chemical_list: D010936:Plant Extracts; D062085:RNA, Long Noncoding; D012333:RNA, Messenger; C497604:SNCA protein, human; D051844:alpha-Synuclein
keywords: Acanthopanax senticosus Harms; Parkinson's disease; lncRNA; neurodegeneration; α-synuclein