title: Bioluminescence Imaging of Adoptively Transferred Lymphocytes During Allogeneic Tumor Rejection.
abstract: OBJECTIVE
  The aim of the present study was to analyze the survival, spatial distribution and proliferation of adoptively transferred lymphocytes in allogeneic tumor rejection.
  
  
  METHODS
  Transgenic β-actin-luc mice that express luciferase were sensitized against SL2 tumors and were used as lymphocyte donors to study the anti-tumor effect in SL2 tumor-bearing lymphocyte-deficient RAG(-/-) mice. Whole-body bioluminescence images of recipient mice were obtained to track the adoptively transferred lymphocytes. Proliferation of lymphocytes was estimated by quantification of photon emission.
  
  
  RESULTS
  T lymphocytes sensitized against allogeneic SL2 tumors cured the majority of SL2 tumor-bearing RAG(-/-) mice. Bioluminescence imaging showed that transferred T lymphocytes survived in the spleen and lymph nodes. Tumor rejection was associated with lymphocyte proliferation and migration to the tumor site.
  
  
  CONCLUSIONS
  Sensitized T lymphocytes from transgenic β-actin-luc mice reject allogeneic SL2 tumors in RAG(-/-) mice and can be tracked in vivo using bioluminescence imaging.
authors.affiliation: Centre for Innovative Medicine, Vilnius, Lithuania.
authors.forename: Jurgita
authors.initials: J
authors.lastname: Juršėnaitė
authors.affiliation: Centre for Innovative Medicine, Vilnius, Lithuania.
authors.forename: Irutė
authors.initials: I
authors.lastname: Girkontaitė
authors.affiliation: Centre for Innovative Medicine, Vilnius, Lithuania.
authors.forename: Mykolas
authors.initials: M
authors.lastname: Mauricas
authors.affiliation: Faculty of Mathematics and Informatics, Vilnius University, Vilnius, Lithuania.
authors.forename: Rimantas
authors.initials: R
authors.lastname: Eidukevičius
authors.affiliation: Centre for Innovative Medicine, Vilnius, Lithuania.
authors.forename: Almantas
authors.initials: A
authors.lastname: Šiaurys
authors.affiliation: Centre for Innovative Medicine, Vilnius, Lithuania Department of Pathology, Forensic Medicine and Pharmacology, Faculty of Medicine, Vilnius University, Vilnius, Lithuania dainius.characiejus@gmail.com.
authors.forename: Dainius
authors.initials: D
authors.lastname: Characiejus
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D006084:Graft Rejection / Q000235:genetics*; D008163:Luminescent Measurements / Q000379:methods*; D008213:Lymphocyte Activation / Q000276:immunology*; D051379:Mice; D008822:Mice, Transgenic
keywords: T lymphocytes; adoptive transfer; bioluminescence imaging; murine tumor model