title: Efficacy Interactions of PEG-DOX-N-acetyl Glucosamine Prodrug Conjugate for Anticancer Therapy.
abstract: Present investigation is exploring structure-biocompatibility interaction of tumour targeted polyethylene glycol (PEG) based drug conjugate of doxorubicin using N-acetyl glucosamine as targeting ligand. The synthesized polymer drug conjugate was evaluated for particle size, zeta potential, molecular weight, haemolysis activity, cytotoxicity, protein binding and in vitro receptor (lectin) binding study. The particle size of synthesized conjugate was observed to be around 30 nm with polydispersability index of 0.213 indicating mono-disperse particles. Fluorescence quenching assay addressed relatively lower binding interactions of polymer drug conjugate to bovine serum albumin in comparison with free doxorubicin which may be governed to the hydrophilicity of polyethylene glycol and N-acetyl glucosamine. The cell compatibility and haemolysis study showed that PEG drug conjugate was nontoxic and biocompatible, which recommends the suitability of polymer drug conjugates for delivering biological active agents systemically. In vitro ligand-lectin receptor binding assays of synthesized targeted polymer conjugate suggest the possibility of promising interaction of N-acetyl glucosamine in vivo. Thus, the study indicated the suitability of N-acetyl glucosamine anchored targeted polymer drug conjugate in delivering bio-therapeutics for specifically targeting to tumour tissues.
authors.affiliation: Department of Pharmaceutical Sciences and Technology, Institute of Chemical Technology, University Under Section 3 of UGC Act 1956, Elite Status and Centre of Excellence - Govt. of Maharashtra, TEQIP Phase II Funded, Mumbai 400 019, India.
authors.forename: Smita K
authors.initials: SK
authors.lastname: Pawar
authors.affiliation: Department of Pharmaceutical Sciences and Technology, Institute of Chemical Technology, University Under Section 3 of UGC Act 1956, Elite Status and Centre of Excellence - Govt. of Maharashtra, TEQIP Phase II Funded, Mumbai 400 019, India. Electronic address: pr.vavia@ictmumbai.edu.in.
authors.forename: Pradeep
authors.initials: P
authors.lastname: Vavia
mesh_terms: D016475:3T3 Cells; D000117:Acetylglucosamine / Q000737:chemistry*; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D004317:Doxorubicin / Q000008:administration & dosage* / Q000737:chemistry; D006801:Humans; D037102:Lectins / Q000378:metabolism; D051379:Mice; D008823:Micelles; D008970:Molecular Weight; D010316:Particle Size; D011092:Polyethylene Glycols / Q000737:chemistry*; D011355:Prodrugs / Q000494:pharmacology*; D013050:Spectrometry, Fluorescence
chemical_list: D000970:Antineoplastic Agents; D037102:Lectins; D008823:Micelles; D011355:Prodrugs; D011092:Polyethylene Glycols; D004317:Doxorubicin; D000117:Acetylglucosamine
keywords: Concanavallin A; Doxorubicin; Fluorescence quenching assay; Haemolysis; Lectin; N-acetyl glucosamine