title: TlR expression profile of human gingival margin-derived stem progenitor cells.
abstract: BACKGROUND
  Gingival margin-derived stem/progenitor cells (G-MSCs) show remarkable periodontal regenerative potential in vivo. During regeneration, G-MSCs may interact with their inflammatory environment via toll-like-receptors (TLRs). The present study aimed to depict the G-MSCs TLRs expression profile.
  
  
  METHODS
  Cells were isolated from free gingival margins, STRO-1-immunomagnetically sorted and seeded to obtain single colony forming units (CFUs). G-MSCs were characterized for CD14, CD34, CD45, CD73, CD90, CD105, CD146 and STRO-1 expression, and for multilineage differentiation potential. Following G-MSCs' incubation in basic or inflammatory medium (IL-1β, IFN-γ, IFN-α, TNF-α) a TLR expression profile was generated.
  
  
  RESULTS
  G-MSCs showed all stem/progenitor cells' characteristics. In basic medium G-MSCs expressed TLRs 1, 2, 3, 4, 5, 6, 7, and 10. The inflammatory medium significantly up-regulated TLRs 1, 2, 4, 5, 7 and 10 and diminished TLR 6 (p≤0.05, Wilcoxon-Signed-Ranks-Test).
  
  
  CONCLUSIONS
  The current study describes for the first time the distinctive TLRs expression profile of G-MSCs under uninflamed and inflamed conditions.
authors.affiliation: Clinic for Conservative Dentistry and Periodontology, School of Dental Medicine, Christian Albrechts-Universität zu Kiel, Arnold-Heller-Str. 3, Haus 26, 24105 Kiel, Germany, karim.fawzy@gmail.com.
authors.forename: Karim
authors.initials: K
authors.lastname: Fawzy-El-Sayed
authors.forename: Mohamed
authors.initials: M
authors.lastname: Mekhemar
authors.forename: Sabine
authors.initials: S
authors.lastname: Adam-Klages
authors.forename: Dietrich
authors.initials: D
authors.lastname: Kabelitz
authors.forename: Christof
authors.initials: C
authors.lastname: Dörfer
mesh_terms: D002478:Cells, Cultured; D005881:Gingiva / Q000166:cytology*; D006801:Humans; D013234:Stem Cells / Q000378:metabolism*; D051193:Toll-Like Receptors / Q000096:biosynthesis*
chemical_list: D051193:Toll-Like Receptors