title: Somatic Embryogenesis Induction and Plant Regeneration in Strawberry Tree (Arbutus unedo L.).
abstract: Somatic embryogenesis is a powerful tool both for cloning and studies of genetic transformation and embryo development. Most protocols for somatic embryogenesis induction start from zygotic embryos or embryonic-derived tissues which do not allow the propagation of elite trees. In the present study, a reliable protocol for somatic embryogenesis induction from adult trees of strawberry tree is described. Leaves from in vitro proliferating shoots were used to induce somatic embryo formation on a medium containing an auxin and a cytokinin. Somatic embryos germinated in a plant growth regulator-free medium.
authors.affiliation: Center for Functional Ecology, Department of Life Sciences, University of Coimbra, Calçada Martim de Freitas, Apartado 3046, 3001-401, Coimbra, Portugal.
authors.forename: João F
authors.initials: JF
authors.lastname: Martins
authors.affiliation: Center for Functional Ecology, Department of Life Sciences, University of Coimbra, Calçada Martim de Freitas, Apartado 3046, 3001-401, Coimbra, Portugal.
authors.forename: Sandra I
authors.initials: SI
authors.lastname: Correia
authors.affiliation: Center for Functional Ecology, Department of Life Sciences, University of Coimbra, Calçada Martim de Freitas, Apartado 3046, 3001-401, Coimbra, Portugal. jorgecan@ci.uc.pt.
authors.forename: Jorge M
authors.initials: JM
authors.lastname: Canhoto
mesh_terms: D031985:Fragaria / Q000235:genetics / Q000254:growth & development*; D005638:Fruit / Q000235:genetics / Q000254:growth & development; D063245:Plant Development / Q000235:genetics; D018520:Plant Shoots / Q000235:genetics / Q000254:growth & development*; D058439:Plant Somatic Embryogenesis Techniques / Q000379:methods*; D030821:Plants, Genetically Modified / Q000235:genetics; D012038:Regeneration / Q000235:genetics; D046509:Tissue Culture Techniques / Q000379:methods*
keywords: Epicormic shoot s; Ericaceae; Fruit; Germination; In vitro; Neglected crops; Shoot proliferation; Somatic embryo