title: Cadmium affects the mitochondrial viability and the acid soluble thiols concentration in liver, kidney, heart and gills of Ancistrus brevifilis (Eigenmann, 1920).
abstract: The freshwater fish Ancistrus brevifilis, which is found in Venezuelan rivers, is considered a potential sentinel fish in ecotoxicological studies. The cadmium (Cd) effect on the mitochondrial viability (MV) and acid soluble thiols levels (AST) in A. brevifilis tissues (liver, kidney, heart, and gill) was evaluated. Forty-two fish with similar sizes and weights were randomly selected, of which 7 fish (with their respective replicate) were exposed for 7 and 30 days to a Cd sublethal concentration (0.1 mg.l(-1)). We determined the MV through a Janus Green B colorimetric assay and we obtained the concentration of AST by Ellman's method. Mitochondrial viability decreased in fish exposed to Cd for 30 days with the liver being the most affected tissue. We also detected a significant decrease in AST levels was in fishes exposed to Cd for 7 days in liver and kidney tissues; these results suggests that AST levels are elevated in some tissues may act as cytoprotective and adaptive alternative mechanism related to the ROS detoxification, maintenance redox status and mitochondrial viability. Organ-specifics variations were observed in both assays. We conclude that the Cd exposure effect on AST levels and MV, vary across fish tissues and is related to the exposure duration, the molecule dynamics in different tissues, the organism and environmental conditions.
authors.affiliation: Laboratorio de proteínas e inmunotoxicidad, Postgrado de Biología Aplicada, Núcleo de Sucre, Universidad de Oriente, Cumaná, Venezuela.
authors.forename: P
authors.initials: P
authors.lastname: Velasquez-Vottelerd
authors.affiliation: Laboratorio de proteínas e inmunotoxicidad, Postgrado de Biología Aplicada, Núcleo de Sucre, Universidad de Oriente, Cumaná, Venezuela.
authors.forename: Y
authors.initials: Y
authors.lastname: Anton
authors.affiliation: Laboratorio de proteínas e inmunotoxicidad, Postgrado de Biología Aplicada, Núcleo de Sucre, Universidad de Oriente, Cumaná, Venezuela.
authors.forename: R
authors.initials: R
authors.lastname: Salazar-Lugo
keywords: Ancistrus brevifilis; Cadmium; Janus Green B; Mitochondrial viability; Soluble thiols