title: A highly sensitive strategy for base excision repair enzyme activity detection based on graphene oxide mediated fluorescence quenching and hybridization chain reaction.
abstract: A novel fluorescent nanosensor has been developed by combining super fluorescence quenching ability of graphene oxide and hybridization chain reaction amplification, which enables highly sensitive detection of base excision repair enzyme activity with a wide dynamic range from 0.0001 to 100 U mL(-1) and a detection limit of 0.00006 U mL(-1).
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, P. R. China. rqyu@hnu.edu.cn jianhuijiang@hnu.edu.cn.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Xi
authors.forename: Jun-Jie
authors.initials: JJ
authors.lastname: Li
authors.forename: Wen-Fang
authors.initials: WF
authors.lastname: Du
authors.forename: Ru-Qin
authors.initials: RQ
authors.lastname: Yu
authors.forename: Jian-Hui
authors.initials: JH
authors.lastname: Jiang
mesh_terms: D004260:DNA Repair; D057075:Enzyme Assays / Q000379:methods*; D006108:Graphite / Q000737:chemistry*; D057230:Limit of Detection; D008958:Models, Molecular; D008968:Molecular Conformation; D036103:Nanotechnology; D009693:Nucleic Acid Hybridization; D010087:Oxides / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D051981:Uracil-DNA Glycosidase / Q000737:chemistry / Q000378:metabolism*
chemical_list: D010087:Oxides; D006108:Graphite; D051981:Uracil-DNA Glycosidase