title: Efficient, Antibiotic Marker-Free Transformation of a Dicot and a Monocot Crop with Glutamate 1-Semialdehyde Aminotransferase Selectable Marker Genes.
abstract: Antibiotic-free, efficient in vitro selection in plant genetic engineering can improve risk perception and speed up pre-market scrutiny of genetically modified crops. We provide a protocol for genetic transformation of two important crops, durum wheat and alfalfa, using a bacterial and a plant-derived selectable marker gene encoding mutated, gabaculine-insensitive glutamate 1-semialdehyde aminotransferase (GSA) enzymes. These methods can potentially be applied, with minor adaptations, to many other monocot and dicot crop plants.
authors.affiliation: Department of Agricultural, Environmental and Food Science, University of Perugia, Borgo XX giugno 74, 06121, Perugia, Italy.
authors.forename: Nicoletta
authors.initials: N
authors.lastname: Ferradini
authors.affiliation: Department of Soil, Plant and Food Science, University of Bari "Aldo Moro", Via G. Amendola 165/A, 70126, Bari, Italy.
authors.forename: Angelica
authors.initials: A
authors.lastname: Giancaspro
authors.affiliation: Department of Agricultural, Environmental and Food Science, University of Perugia, Borgo XX giugno 74, 06121, Perugia, Italy.|ENEA Casaccia Research Center, via Anguillarese 301, 00123, Roma, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Nicolia
authors.affiliation: Department of Soil, Plant and Food Science, University of Bari "Aldo Moro", Via G. Amendola 165/A, 70126, Bari, Italy.
authors.forename: Agata
authors.initials: A
authors.lastname: Gadaleta
authors.affiliation: Department of Agricultural, Environmental and Food Science, University of Perugia, Borgo XX giugno 74, 06121, Perugia, Italy.
authors.forename: Fabio
authors.initials: F
authors.lastname: Veronesi
authors.affiliation: Department of Agricultural, Environmental and Food Science, University of Perugia, Borgo XX giugno 74, 06121, Perugia, Italy. daniele.rosellini@unipg.it.
authors.forename: Daniele
authors.initials: D
authors.lastname: Rosellini
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D005818:Genetic Engineering / Q000379:methods*; D005819:Genetic Markers; D019751:Intramolecular Transferases / Q000235:genetics*; D000455:Medicago sativa / Q000235:genetics* / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics; D030821:Plants, Genetically Modified; D014170:Transformation, Genetic; D014908:Triticum / Q000235:genetics* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D005819:Genetic Markers; D010940:Plant Proteins; D019751:Intramolecular Transferases; C061049:glutamate-1-semialdehyde 2,1-aminomutase
keywords: Alfalfa; Durum wheat; GMO; Gabaculine; HemL; MsGSAgr; Non-antibiotic selection; Plant genetic engineering