title: Limitation of tuning the antibody-antigen reaction by changing the value of pH and its consequence for hyperthermia.
abstract: Distribution of the isoelectric point (pI) was calculated for the hypervariable regions of Fab fragments of the antibody molecules, which structure is annotated in the structural antibody database SabDab. The distribution is consistent with the universal for all organisms dividing the proteome into two sets of acidic and basic proteins. It shows the additional fine structure in a form of the narrow-sized peaks of pI values. This is an explanation why a small change of the environmental pH can have a strong effect on the antibody-antigen affinity. To show this, a typical enzyme-linked immunospecific assay experiment for testing the reaction of goat anti-human IgA antibodies with human IgA immunoglobulins of saliva as antigens was modified in such a way that Fe3O4magnetic nanoparticles were added to PBS buffer. The magnetic nanoparticles were remotely heated by the radio frequency magnetic field providing the local change of temperature and pH. It was observed that short times of the heating were significantly increasing the antibody-antigen binding strength while it was not the case for a longer time. The finding discussed in the study can be useful for biopharmaceuticals using antibodies, the immunoassay techniques as well as for control over the use of hyperthermia.
authors.affiliation: Institute of Genetics and Microbiology, University of Wrocław, ul. Przybyszewskiego 63/77, 51-148 Wrocław, Poland;
authors.forename: J
authors.initials: J
authors.lastname: Mleczko
authors.affiliation: Faculty of Biological Sciences, University of Zielona Góra, ul. Szafrana 1, 65-516 Zielona Góra, Poland; and.
authors.forename: A
authors.initials: A
authors.lastname: Defort
authors.affiliation: Faculty of Biological Sciences, University of Zielona Góra, ul. Szafrana 1, 65-516 Zielona Góra, Poland; and.
authors.forename: J J
authors.initials: JJ
authors.lastname: Kozioł
authors.affiliation: Institute of Physics, University of Zielona Góra, ul. Szafrana 4a, 65-516 Zielona Góra, Poland.
authors.forename: T T
authors.initials: TT
authors.lastname: Nguyen
authors.affiliation: Faculty of Biological Sciences, University of Zielona Góra, ul. Szafrana 1, 65-516 Zielona Góra, Poland; and.
authors.forename: A
authors.initials: A
authors.lastname: Mirończyk
authors.affiliation: Institute of Physics, University of Zielona Góra, ul. Szafrana 4a, 65-516 Zielona Góra, Poland.
authors.forename: B
authors.initials: B
authors.lastname: Zapotoczny
authors.affiliation: Faculty of Biological Sciences, University of Zielona Góra, ul. Szafrana 1, 65-516 Zielona Góra, Poland; and.
authors.forename: J
authors.initials: J
authors.lastname: Nowak-Jary
authors.affiliation: Faculty of Biological Sciences, University of Zielona Góra, ul. Szafrana 1, 65-516 Zielona Góra, Poland; and.
authors.forename: E
authors.initials: E
authors.lastname: Gronczewska
authors.affiliation: Institute of Physics, University of Zielona Góra, ul. Szafrana 4a, 65-516 Zielona Góra, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Marć
authors.affiliation: Institute of Physics, University of Zielona Góra, ul. Szafrana 4a, 65-516 Zielona Góra, Poland M.Dudek@if.uz.zgora.pl.
authors.forename: M R
authors.initials: MR
authors.lastname: Dudek
mesh_terms: D000906:Antibodies / Q000737:chemistry*; D000915:Antibody Affinity; D000937:Antigen-Antibody Reactions; D000941:Antigens / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006358:Hot Temperature; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007070:Immunoglobulin A / Q000276:immunology; D007140:Immunoglobulin Fab Fragments / Q000737:chemistry*; D007526:Isoelectric Point; D058185:Magnetite Nanoparticles; D012463:Saliva / Q000276:immunology
chemical_list: D000906:Antibodies; D000941:Antigens; D007070:Immunoglobulin A; D007140:Immunoglobulin Fab Fragments; D058185:Magnetite Nanoparticles
keywords: Raman spectroscopy; antibody-antigen complex; isoelectric point; magnetic hyperthermia; magnetic nanoparticles