title: Decreased Frequency of IL-17F rs763780 Site Allele G is Associated With Genetic Susceptibility to Immune Thrombocytopenia in a Chinese Population.
abstract: Interleukin 17F (IL-17F) is an inflammatory cytokine that plays an important role in autoimmune disease by inducing the expression of multiple chemokines, cytokines, and adhesion molecules. In vitro functional analysis revealed that IL-17F rs763780 polymorphism is associated with IL-17 expression and activity. Thus, considering the abnormal percentage of T helper 17 cells in patients with primary immune thrombocytopenia (ITP), we speculated there was a possible association between the IL-17F rs763780 polymorphisms and genetic susceptibility to ITP in a Chinese Han population. A total of 165 patients with ITP and 149 healthy controls were included in this study, and IL-17F rs763780 polymorphisms were analyzed by a polymerase chain reaction-restriction fragment length polymorphism system. The results showed that the frequency of the IL-17F rs763780 G allele in total patients with ITP or patients with chronic ITP was significantly lower than in normal controls (total ITP 3.6% vs controls 7.7%, P = .026; chronic ITP 3.5% vs controls 7.7%, P = .031). However, no significant difference in genotype frequencies was found among total patients with ITP, patients with chronic ITP, and normal controls. We further analyzed the association of IL-17F polymorphisms with clinical parameters of patients with ITP, and no association revealed between gene distribution and first onset age, clinical therapy response to glucocorticoids, or disease course. What's more, an evident discrepancy with allelic frequencies was observed between female patients with ITP and gender-matched controls. In conclusion, IL-17F rs763780 polymorphisms may be associated with the development of ITP in a Chinese Han population.
authors.affiliation: 1 State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Disease Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin, China.
authors.forename: Huiyuan
authors.initials: H
authors.lastname: Li
authors.affiliation: 2 Department of Hematology, the Second Affiliated Hospital of Kunming Medical University, Kunming, China.|3 Yunnan Molecular Diagnosis Centre, the Second Affiliated Hospital of Kunming Medical University, Kunming, China.
authors.forename: Zeping
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: 3 Yunnan Molecular Diagnosis Centre, the Second Affiliated Hospital of Kunming Medical University, Kunming, China.
authors.forename: Wenlin
authors.initials: W
authors.lastname: Tai
authors.affiliation: 4 Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Kunming Medical University, Kunming, China.
authors.forename: Weiyang
authors.initials: W
authors.lastname: Feng
authors.affiliation: 1 State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Disease Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin, China.
authors.forename: Donglei
authors.initials: D
authors.lastname: Zhang
authors.affiliation: 2 Department of Hematology, the Second Affiliated Hospital of Kunming Medical University, Kunming, China.
authors.forename: Xueping
authors.initials: X
authors.lastname: Gu
authors.affiliation: 1 State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Disease Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin, China.
authors.forename: Renchi
authors.initials: R
authors.lastname: Yang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D044466:Asian People / Q000235:genetics*; D002648:Child; D002675:Child, Preschool; D002681:China / Q000453:epidemiology; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease / Q000453:epidemiology / Q000235:genetics*; D006801:Humans; D020381:Interleukin-17 / Q000235:genetics* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D016553:Purpura, Thrombocytopenic, Idiopathic / Q000453:epidemiology / Q000235:genetics* / Q000276:immunology; D058504:Th17 Cells / Q000276:immunology
chemical_list: C440021:IL17F protein, human; D020381:Interleukin-17
keywords: genetic susceptibility; interleukin 17F; polymorphism; primary immune thrombocytopenia