title: Cloning of the Major Capsid Protein (MCP) of Grouper Iridovirus of Taiwan (TGIV) and Preliminary Evaluation of a Recombinant MCP Vaccine against TGIV.
abstract: Fish iridoviruses cause systemic diseases with high mortality in various species of wild and farm-raised fish, resulting in severe economic losses. In 1998, we isolated a new epizootic iridovirus in cultured grouper (Epinephelus sp.) in Taiwan, thus named as grouper iridovirus of Taiwan (TGIV). We report here the cloning of TGIV major capsid protein (MCP). Phylogenetic analysis of the iridoviral MCPs confirmed the classification of TGIV into the Megalocytivirus genus. Recombinant TGIV MCP and GIV MCP were then generated to produce polyclonal antibodies. Western blot analysis revealed that the two antisera were species-specific, indicating no common epitope shared by the MCPs of the two viruses. We further assayed the potency of a subunit vaccine containing recombinant TGIV MCP. The vaccine effectively protected grouper from TGIV infection. The result demonstrated that MCP is a suitable antigen for anti-TGIV vaccines.
authors.affiliation: Department of Aquaculture, National Taiwan Ocean University, Keelung 20224, Taiwan. duo.liu@msa.hinet.net.
authors.forename: Hsin-I
authors.initials: HI
authors.lastname: Liu
authors.affiliation: Department of Aquaculture, National Taiwan Ocean University, Keelung 20224, Taiwan. ppchiou@mail.ntou.edu.tw.|Center of Excellence for the Oceans, National Taiwan Ocean University, No. 2, Pei-Ning Rd., Keelung 20224, Taiwan. ppchiou@mail.ntou.edu.tw.
authors.forename: Pinwen Peter
authors.initials: PP
authors.lastname: Chiou
authors.affiliation: Department of Aquaculture, National Taiwan Ocean University, Keelung 20224, Taiwan. hygong@mail.ntou.edu.tw.|Center of Excellence for the Oceans, National Taiwan Ocean University, No. 2, Pei-Ning Rd., Keelung 20224, Taiwan. hygong@mail.ntou.edu.tw.
authors.forename: Hong-Yi
authors.initials: HY
authors.lastname: Gong
authors.affiliation: Department of Aquaculture, National Taiwan Ocean University, Keelung 20224, Taiwan. hychou@mail.ntou.edu.tw.|Center of Excellence for the Oceans, National Taiwan Ocean University, No. 2, Pei-Ning Rd., Keelung 20224, Taiwan. hychou@mail.ntou.edu.tw.
authors.forename: Hsin-Yiu
authors.initials: HY
authors.lastname: Chou
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D036022:Capsid Proteins / Q000235:genetics* / Q000276:immunology*; D003001:Cloning, Molecular; D005393:Fish Diseases / Q000517:prevention & control*; D015870:Gene Expression; D007114:Immunization; D017994:Iridovirus / Q000235:genetics* / Q000276:immunology*; D011817:Rabbits; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism; D017422:Sequence Analysis, DNA; D013624:Taiwan; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics* / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D036022:Capsid Proteins; D011994:Recombinant Proteins; D014614:Vaccines, Synthetic
keywords: grouper iridovirus of Taiwan (TGIV); major capsid protein (MCP); recombinant vaccine