title: Multiresidue method for the simultaneous determination of veterinary medicinal products, feed additives and illegal dyes in eggs using liquid chromatography-tandem mass spectrometry.
abstract: A multiclass method was developed for the simultaneous determination of 120 analytes in fresh eggs. The method covers the analytes from the groups of tetracyclines (6), fluoroquinolones (11), sulphonamides (17), nitroimidazoles (9), amphenicols (2), cephalosporins (7), penicillins (8), macrolides (8), benzimidazoles (20), coccidiostats (14), insecticides (3), dyes (12) and others (3). Samples were extracted using 0.1% formic acid in acetonitrile:water (8:2) with the addition of EDTA and cleaned using solid phase extraction with Hybrid SPE cartridges. The chromatographic separation was achieved on C8 column using mobile phase consisting of (A) methanol:acetonitrile (8:2) - (B) 0.1% formic acid in a gradient mode. Validation results according to the Commission Decision 2002/657/EC are as follows: linearity (r⩾0.99), recovery (75-108%), repeatability (CV 1.60-15.9%), reproducibility (CV 2.60-15%), decision limit (CCα 2.25-1156 μg/kg) and detection capability (CCβ 2.04-1316 μg/kg). The presented method was used for analysis of 150 real eggs samples taken from monitoring control program.
authors.affiliation: Department of Pharmacology and Toxicology, National Veterinary Research Institute, Partyzantow 57, 24-100 Pulawy, Poland. Electronic address: marta.piatkowska@piwet.pulawy.pl.
authors.forename: Marta
authors.initials: M
authors.lastname: Piatkowska
authors.affiliation: Department of Pharmacology and Toxicology, National Veterinary Research Institute, Partyzantow 57, 24-100 Pulawy, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Jedziniak
authors.affiliation: Department of Pharmacology and Toxicology, National Veterinary Research Institute, Partyzantow 57, 24-100 Pulawy, Poland.
authors.forename: Jan
authors.initials: J
authors.lastname: Zmudzki
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003049:Coccidiostats / Q000737:chemistry / Q000302:isolation & purification; D004396:Coloring Agents / Q000737:chemistry* / Q000302:isolation & purification; D004350:Drug Residues / Q000737:chemistry* / Q000302:isolation & purification; D004531:Eggs / Q000032:analysis*; D005503:Food Additives / Q000737:chemistry* / Q000302:isolation & purification; D018942:Macrolides / Q000737:chemistry / Q000302:isolation & purification; D015203:Reproducibility of Results; D052616:Solid Phase Extraction / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*; D019155:Veterinary Drugs / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D000900:Anti-Bacterial Agents; D003049:Coccidiostats; D004396:Coloring Agents; D005503:Food Additives; D018942:Macrolides; D019155:Veterinary Drugs
keywords: Coccidiostats; Dyes; Eggs; LC–MS/MS; Multiclass; Multiresidue; Veterinary drugs