title: Mesorhizobium calcicolasp. nov.,Mesorhizobium waitakensesp. nov.,Mesorhizobium sophoraesp. nov.,Mesorhizobium newzealandensesp. nov. andMesorhizobium kowhaiisp. nov. isolated fromSophoraroot nodules.
abstract: In total, 31 strains of Gram-stain-negative, rod-shaped bacteria were isolated fromSophoraroot nodules and authenticated as rhizobia on this host. Based on 16S rRNA gene phylogeny, they were shown to belong to the genusMesorhizobium, with the representative strains ICMP 19560T, ICMP 19523T, ICMP 19535T, ICMP 19545Tand ICMP 19512Tbeing related most closely toMesorhizobium sangaiiSCAU7T(99.9-99.6 % similarity),Mesorhizobium cantuarienseICMP 19515T(99.7-99.6 %) andMesorhizobium ciceriUMP-CA7T(99.7-99.5 %). Additionally, the novel strains formed distinct groups based on housekeeping gene sequence analysis and were closely related toMesorhizobium waimenseICMP 19557T(93.5-94.9, 92.5-95.6 and 94.2-96.0 %),M. cantuarienseICMP 19515T(93.1-97.7, 93.5-95.4 and 94.8-96.8 %) andM. ciceriUMP-CA7T(93.2-97.2, 94.6-96.8 and 95.5-97.3 %) forglnII,recAandrpoB, respectively. Chemotaxonomic data supported the assignment of the strains to the genusMesorhizobium, and DNA-DNA hybridizations, matrix-assisted laser desorption/ionization time-of-flight MS analysis, enterobacterial repetitive intergenic consensus PCR, physiological and biochemical tests allowed the genotypic and phenotypic differentiation from their nearest neighbouring species. Therefore, these strains represent five novel species for which the namesMesorhizobium calcicolasp. nov. (type strain ICMP 19560T= LMG 28224T= HAMBI 3609T),Mesorhizobium waitakensesp. nov. (type strain ICMP 19523T= LMG 28227T= HAMBI 3605T),Mesorhizobium sophoraesp. nov. (type strain ICMP 19535T= LMG 28223T= HAMBI 3606T),Mesorhizobium newzealandensesp. nov. (type strain ICMP 19545T= LMG 28226T= HAMBI 3607T) andMesorhizobium kowhaiisp. nov. (type strain ICMP 19512T= LMG 28222T= HAMBI 3603T) are proposed.
authors.affiliation: Centre for Rhizobium Studies,Murdoch University, Western Australia 6150,Australia.|Laboratory of Microbiology, Department of Biochemistry and Microbiology,Ghent University, K.L. Ledeganckstraat 35, B-9000 Ghent,Belgium.
authors.forename: Sofie E
authors.initials: SE
authors.lastname: De Meyer
authors.affiliation: Faculty of Agriculture and Life Sciences,Lincoln University, Lincoln 7647,New Zealand.
authors.forename: Heng Wee
authors.initials: HW
authors.lastname: Tan
authors.affiliation: Faculty of Agriculture and Life Sciences,Lincoln University, Lincoln 7647,New Zealand.
authors.forename: Mitchell
authors.initials: M
authors.lastname: Andrews
authors.affiliation: Allan Herbarium,Landcare Research, Lincoln 7640,New Zealand.
authors.forename: Peter B
authors.initials: PB
authors.lastname: Heenan
authors.affiliation: Laboratory of Microbiology, Department of Biochemistry and Microbiology,Ghent University, K.L. Ledeganckstraat 35, B-9000 Ghent,Belgium.
authors.forename: Anne
authors.initials: A
authors.lastname: Willems