title: Characterization of a Newly Isolated Marine Fungus Aspergillus dimorphicus for Optimized Production of the Anti-Tumor Agent Wentilactones.
abstract: The potential anti-tumor agent wentilactones were produced by a newly isolated marine fungus Aspergillus dimorphicus. This fungus was derived from deep-sea sediment and identified by polyphasic approach, combining phenotypic, molecular, and extrolite profiles. However, wentilactone production was detected only under static cultures with very low yields. In order to improve wentilactone production, culture conditions were optimized using the response surface methodology. Under the optimal static fermentation conditions, the experimental values were closely consistent with the prediction model. The yields of wentilactone A and B were increased about 11-fold to 13.4 and 6.5 mg/L, respectively. The result was further verified by fermentation scale-up for wentilactone production. Moreover, some small-molecule elicitors were found to have capacity of stimulating wentilactone production. To our knowledge, this is first report of optimized production of tetranorlabdane diterpenoids by a deep-sea derived marine fungus. The present study might be valuable for efficient production of wentilactones and fundamental investigation of the anti-tumor mechanism of norditerpenoids.
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Nanhai Road 7, Qingdao 266071, China. xrxurui@yeah.net.|College of Earth Science, University of Chinese Academy of Sciences, Yuquan Road 19A, Beijing 100049, China. xrxurui@yeah.net.
authors.forename: Rui
authors.initials: R
authors.lastname: Xu
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Nanhai Road 7, Qingdao 266071, China. aericxu@gmail.com.
authors.forename: Gang-Ming
authors.initials: GM
authors.lastname: Xu
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Nanhai Road 7, Qingdao 266071, China. lixmqd@aliyun.com.
authors.forename: Xiao-Ming
authors.initials: XM
authors.lastname: Li
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Nanhai Road 7, Qingdao 266071, China. lichunshun@qdio.ac.cn.
authors.forename: Chun-Shun
authors.initials: CS
authors.lastname: Li
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Nanhai Road 7, Qingdao 266071, China. wangbg@qdio.ac.cn.
authors.forename: Bin-Gui
authors.initials: BG
authors.lastname: Wang
mesh_terms: D000970:Antineoplastic Agents / Q000302:isolation & purification*; D001230:Aspergillus / Q000302:isolation & purification / Q000378:metabolism*; D003470:Culture Media; D005285:Fermentation; D019015:Geologic Sediments / Q000382:microbiology*; D006576:Heterocyclic Compounds, 4 or More Rings / Q000302:isolation & purification*
chemical_list: D000970:Antineoplastic Agents; D003470:Culture Media; D006576:Heterocyclic Compounds, 4 or More Rings; C454809:wentilactone B
keywords: Aspergillus dimorphicus; anti-tumor agent; optimization; wentilactones