title: A fast click-slow release strategy towards the HPLC-free synthesis of RNA.
abstract: A general strategy for purification of oligonucleotides synthesized by solid phase synthesis is described. It is based on a recently developed concept involving a bio-orthogonal inverse electron demand Diels-Alder reaction between trans-cyclooctene and tetrazine, termed 'click-to-release'. The strategy has been applied towards the synthesis and purification of a model hairpin RNA strand, as well as a 34 nt long aptamer.
authors.affiliation: Department of Chemistry, SUNY, University at Albany, 1400 Washington Avenue, Albany, NY 1222, USA. mroyzen@albany.edu.
authors.forename: E
authors.initials: E
authors.lastname: Agustin
authors.affiliation: Department of Chemistry, SUNY, University at Albany, 1400 Washington Avenue, Albany, NY 1222, USA. mroyzen@albany.edu.
authors.forename: P N
authors.initials: PN
authors.lastname: Asare Okai
authors.affiliation: Department of Chemistry, SUNY, University at Albany, 1400 Washington Avenue, Albany, NY 1222, USA. mroyzen@albany.edu.
authors.forename: I
authors.initials: I
authors.lastname: Khan
authors.affiliation: Department of Chemistry, SUNY, University at Albany, 1400 Washington Avenue, Albany, NY 1222, USA. mroyzen@albany.edu.
authors.forename: M R
authors.initials: MR
authors.lastname: Miller
authors.affiliation: Department of Chemistry, SUNY, University at Albany, 1400 Washington Avenue, Albany, NY 1222, USA. mroyzen@albany.edu.
authors.forename: R
authors.initials: R
authors.lastname: Wang
authors.affiliation: Department of Chemistry, SUNY, University at Albany, 1400 Washington Avenue, Albany, NY 1222, USA. mroyzen@albany.edu.
authors.forename: J
authors.initials: J
authors.lastname: Sheng
authors.affiliation: Department of Chemistry, SUNY, University at Albany, 1400 Washington Avenue, Albany, NY 1222, USA. mroyzen@albany.edu.
authors.forename: M
authors.initials: M
authors.lastname: Royzen
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D057930:Click Chemistry; D012313:RNA / Q000138:chemical synthesis*
chemical_list: D012313:RNA