title: Antileishmanial diterpenoid alkaloids from Aconitum spicatum (Bruhl) Stapf.
abstract: The crude extracts of tubers of Aconitum spicatum (Bruhl) Stapf were investigated for in vitro antileishmanial activity against Leishmania major. The dichloromethane extract at pH 2.5 showed antileishmanial activity with IC50value of 27.10 ± 0.0 μg/mL. Chromatographic purification of the dichloromethane extract led to isolation of three C-19 norditerpenoid alkaloids indaconitine (1), chasmaconitine (2) and ludaconitine (3). Compounds 3 and 2 showed antileishmanial activity with IC50= 36.10 ± 3.4 and 56.30 ± 2.1 μg/mL, respectively. Compound 1 was less effective (IC50> 100 μg/mL). The cytotoxicity of compounds 1, 2 and 3 studied against MCF7, HeLa and PC3 cancer cell lines and 3T3 normal fibroblast cell line did not show cytotoxicity at 30 μM.
authors.affiliation: a Faculty of Science , Nepal Academy of Science and Technology , Lalitpur , Nepal.
authors.forename: Sajan L
authors.initials: SL
authors.lastname: Shyaula
authors.affiliation: b Department of Chemistry, Tri-Chandra Multiple Campus , Tribhuvan University , Kathmandu , Nepal.
authors.forename: Tensangmu
authors.initials: T
authors.lastname: Tamang
authors.affiliation: c HEJ Research Institute of Chemistry, International Center for Chemical and Biological Sciences , University of Karachi , Karachi , Pakistan.
authors.forename: Nida
authors.initials: N
authors.lastname: Ghouri
authors.affiliation: c HEJ Research Institute of Chemistry, International Center for Chemical and Biological Sciences , University of Karachi , Karachi , Pakistan.
authors.forename: Achyut
authors.initials: A
authors.lastname: Adhikari
authors.affiliation: b Department of Chemistry, Tri-Chandra Multiple Campus , Tribhuvan University , Kathmandu , Nepal.
authors.forename: Shanti
authors.initials: S
authors.lastname: Marasini
authors.affiliation: a Faculty of Science , Nepal Academy of Science and Technology , Lalitpur , Nepal.
authors.forename: Gan B
authors.initials: GB
authors.lastname: Bajracharya
authors.affiliation: a Faculty of Science , Nepal Academy of Science and Technology , Lalitpur , Nepal.
authors.forename: Mangala D
authors.initials: MD
authors.lastname: Manandhar
authors.affiliation: c HEJ Research Institute of Chemistry, International Center for Chemical and Biological Sciences , University of Karachi , Karachi , Pakistan.|d Faculty of Science, Department of Biochemistry , King Abdulaziz University , Jeddah , Saudi Arabia.
authors.forename: Muhammad Iqbal
authors.initials: MI
authors.lastname: Choudhary
keywords: Aconitum spicatum; Leishmania major; antileishmanial activity; diterpenoid alkaloids