title: Inflammatory mediators, tumor necrosis factor-α and interferon-γ, induce EMT in human PTC cell lines.
abstract: Inflammatory mediators, tumor necrosis factor (TNF)-α and interferon (IFN)-γ, promote adverse outcomes in numerous types of cancer; however, their role in papillary thyroid cancer (PTC) remains unclear. The aim of the present study was to investigate the influence of TNF-α and IFN-γ on the migration, invasion and epithelial-mesenchymal transition (EMT) of the three PTC cell lines, TPC-1, BCPAP and K1. The effect of TNF-α and IFN-γ on cell migration and invasion was assessed by wound-healing and Transwell assays. In addition, the mRNA and protein expression levels of the EMT makers, E-cadherin, N-cadherin and vimentin, were analyzed using reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and immunoblot analysis. The wound-healing and Transwell experiments revealed that TNF-α and IFN-γ increased the migratory and invasive behavior of PTC cells (P<0.05). RT-qPCR revealed that TNF-α and IFN-γ downregulated E-cadherin mRNA, while they upregulated N-cadherin and vimentin mRNA expression levels. These results were further confirmed by the immunoblot analysis. The results of the present study suggest that TNF-α and IFN-γ induce EMT and malignant progression in human PTC cells.
authors.affiliation: Department of Endocrinology and Metabolism, Endocrine Institute and Liaoning Provincial Key Laboratory of Endocrine Diseases, The First Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China.
authors.forename: Nannan
authors.initials: N
authors.lastname: Lv
authors.affiliation: Research Center of Stem Cell, He Eye Hospital, He University, Shenyang, Liaoning 110163, P.R. China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Department of Endocrinology and Metabolism, Endocrine Institute and Liaoning Provincial Key Laboratory of Endocrine Diseases, The First Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China.
authors.forename: Haixia
authors.initials: H
authors.lastname: Guan
authors.affiliation: Department of Endocrinology and Metabolism, Endocrine Institute and Liaoning Provincial Key Laboratory of Endocrine Diseases, The First Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China.
authors.forename: Dan
authors.initials: D
authors.lastname: Wu
authors.affiliation: Department of Endocrinology and Metabolism, Endocrine Institute and Liaoning Provincial Key Laboratory of Endocrine Diseases, The First Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China.
authors.forename: Shuangning
authors.initials: S
authors.lastname: Ding
authors.affiliation: Department of Endocrinology and Metabolism, Endocrine Institute and Liaoning Provincial Key Laboratory of Endocrine Diseases, The First Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China.
authors.forename: Weiping
authors.initials: W
authors.lastname: Teng
authors.affiliation: Department of Endocrinology and Metabolism, Endocrine Institute and Liaoning Provincial Key Laboratory of Endocrine Diseases, The First Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China.
authors.forename: Zhongyan
authors.initials: Z
authors.lastname: Shan
keywords: epithelial-mesenchymal transition; inflammation; invasion; migration; papillary thyroid cancer