title: Corosolic acid ameliorates acute inflammation through inhibition of IRAK-1 phosphorylation in macrophages.
abstract: Corosolic acid (CA), a triterpenoid compound isolated from Lagerstroemia speciosa L. (Banaba) leaves, exerts anti-inflammatory effects by regulating phosphorylation of interleukin receptor-associated kinase (IRAK)-2 via the NF-κB cascade. However, the protective effect of CA against endotoxic shock has not been reported. LPS (200 ng/mL, 30 min) induced phosphorylation of IRAK-1 and treatment with CA (10 μM) significantly attenuated this effect. In addition, CA also reduced protein levels of NLRP3 and ASC which are the main components of the inflammasome in BMDMs. LPS-induced inflammasome assembly through activation of IRAK-1 was down-regulated by CA challenge. Treatment with Bay11-7082, an inhibitor of IκB-α, had no effect on CA-mediated inhibition of IRAK-1 activation, indicating that CA-mediated attenuation of IRAK-1 phosphorylation was independent of NF-κB signaling. These results demonstrate that CA ameliorates acute inflammation in mouse BMDMs and CA may be useful as a pharmacological agent to prevent acute inflammation. [BMB Reports 2016; 49(5): 276-281].
authors.affiliation: Department of Physiology, Keimyung University School of Medicine, Daegu 42601, Korea.
authors.forename: Seung-Jae
authors.initials: SJ
authors.lastname: Kim
authors.affiliation: Department of Biochemistry, Lee Gil Ya Cancer and Diabetes Institute, Gachon University, Incheon 21999; Gachon Medical Research Institute, Gill Hospital, Incheon 21936, Korea.
authors.forename: Ji-Young
authors.initials: JY
authors.lastname: Cha
authors.affiliation: Department of Physiology, Keimyung University School of Medicine, Daegu 42601, Korea.
authors.forename: Hye Suk
authors.initials: HS
authors.lastname: Kang
authors.affiliation: Department of Physiology, Keimyung University School of Medicine, Daegu 42601, Korea.
authors.forename: Jae-Ho
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Severance Biomedical Science Institute, Yonsei University College of Medicine, Seoul 03722, Korea.
authors.forename: Ji Yoon
authors.initials: JY
authors.lastname: Lee
authors.affiliation: Department of Physiology, Keimyung University School of Medicine, Daegu 42601, Korea.
authors.forename: Jae-Hyung
authors.initials: JH
authors.lastname: Park
authors.affiliation: Department of Physiology, Keimyung University School of Medicine, Daegu 42601, Korea.
authors.forename: Jae-Hoon
authors.initials: JH
authors.lastname: Bae
authors.affiliation: Department of Physiology, Keimyung University School of Medicine, Daegu 42601, Korea.
authors.forename: Dae-Kyu
authors.initials: DK
authors.lastname: Song
authors.affiliation: Department of Physiology, Keimyung University School of Medicine, Daegu 42601, Korea.
authors.forename: Seung-Soon
authors.initials: SS
authors.lastname: Im
mesh_terms: D000208:Acute Disease; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D001854:Bone Marrow Cells / Q000473:pathology; D002432:Cecum / Q000473:pathology; D058847:Inflammasomes / Q000378:metabolism; D007249:Inflammation / Q000188:drug therapy* / Q000473:pathology*; D053592:Interleukin-1 Receptor-Associated Kinases / Q000378:metabolism*; D008026:Ligation; D008070:Lipopolysaccharides; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008810:Mice, Inbred C57BL; D016328:NF-kappa B / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D011677:Punctures; D015398:Signal Transduction / Q000187:drug effects; D051197:Toll-Like Receptor 4 / Q000378:metabolism; D014315:Triterpenes / Q000494:pharmacology / Q000627:therapeutic use*
chemical_list: D000893:Anti-Inflammatory Agents; D058847:Inflammasomes; D008070:Lipopolysaccharides; D016328:NF-kappa B; D051197:Toll-Like Receptor 4; D014315:Triterpenes; C113861:corosolic acid; D053592:Interleukin-1 Receptor-Associated Kinases