title: Isolation and morphological characterization of ovine amniotic fluid mesenchymal stem cells.
abstract: Mesenchymal stem cells (MSCs) are one of the most promising cell populations for tissue engineering and regenerative medicine. Of utmost importance to MSC research is identification of MSC sources that are easily obtainable and stable. Several studies have shown that MSCs can be isolated from amniotic fluid. The sheep is one of the main types of farm animal, and it has many biophysical and biochemical similarities to humans. Here, we obtained MSCs from ovine amniotic fluid and determined the expansion capacity, surface and intracellular marker expression, karyotype, and multilineage differentiation ability of these ovine amniotic fluid mesenchymal stem cells (oAF-MSCs). Moreover, expression levels of differentiation markers were measured using reverse transcription-qPCR (RT-qPCR). Our phenotypic analysis shows that the isolated oAF-MSCs are indeed MSCs.
authors.affiliation: College of Life Sciences, Inner Mongolia University, Inner Mongolia, Hohhot 010021, P.R.China.
authors.forename: Yunyun
authors.initials: Y
authors.lastname: Tian
authors.forename: Li
authors.initials: L
authors.lastname: Tao
authors.forename: Siriguleng
authors.initials: S
authors.lastname: Zhao
authors.forename: Dapeng
authors.initials: D
authors.lastname: Tai
authors.forename: Dongjun
authors.initials: D
authors.lastname: Liu
authors.forename: Pengxia
authors.initials: P
authors.lastname: Liu
mesh_terms: D050156:Adipogenesis; D000653:Amniotic Fluid / Q000166:cytology*; D000818:Animals; D015703:Antigens, CD / Q000032:analysis; D000954:Antigens, Surface / Q000032:analysis; D002454:Cell Differentiation / Q000235:genetics; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D020219:Chondrogenesis; D005260:Female; D006801:Humans; D059785:Karyotype; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism; D010012:Osteogenesis; D010641:Phenotype; D011247:Pregnancy; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012756:Sheep
chemical_list: D015703:Antigens, CD; D000954:Antigens, Surface