title: Liposome-Loaded Cell Backpacks.
abstract: Cell backpacks, or micron-scale patches of a few hundred nanometers in thickness fabricated by layer-by-layer (LbL) assembly, are potentially useful vehicles for targeted drug delivery on the cellular level. In this work, echogenic liposomes (ELIPs) containing the anticancer drug doxorubicin (DOX) are embedded into backpacks through electrostatic interactions and LbL assembly. Poly(allylamine hydrochloride)/poly(acrylic acid) (PAH/PAA)n , and poly(diallyldimethylammonium chloride)/poly(styrene sulfonate) (PDAC/SPS)n film systems show the greatest ELIP incorporation of the films studied while maintaining the structural integrity of the vesicles. The use of ELIPs for drug encapsulation into backpacks facilitates up to three times greater DOX loading compared to backpacks without ELIPs. Cytotoxicity studies reveal that monocyte backpack conjugates remain viable even after 72 h, demonstrating promise as drug delivery vehicles. Because artificial vesicles can load many different types of drugs, ELIP containing backpacks offer a unique versatility for broadening the range of possible applications for cell backpacks.
authors.affiliation: School of Pharmaceutical Sciences, University of Sao Paulo, USP Sao Paulo, SP, 05508-900, Brazil.|Department of Materials Science and Engineering, Massachusetts Institute of Technology (MIT), Cambridge, M,A, 02139, USA.
authors.forename: Roberta
authors.initials: R
authors.lastname: Polak
authors.affiliation: Department of Chemical Engineering, Massachusetts Institute of Technology (MIT), Cambridge, MA, 02139, USA.
authors.forename: Rosanna M
authors.initials: RM
authors.lastname: Lim
authors.affiliation: School of Chemical Engineering, University of Campinas, UNICAMP, Campinas, SP, 13083-852, Brazil.
authors.forename: Marisa M
authors.initials: MM
authors.lastname: Beppu
authors.affiliation: School of Pharmaceutical Sciences, University of Sao Paulo, USP Sao Paulo, SP, 05508-900, Brazil.
authors.forename: Ronaldo N M
authors.initials: RN
authors.lastname: Pitombo
authors.affiliation: Department of Chemical Engineering, Massachusetts Institute of Technology (MIT), Cambridge, MA, 02139, USA.
authors.forename: Robert E
authors.initials: RE
authors.lastname: Cohen
authors.affiliation: Department of Materials Science and Engineering, Massachusetts Institute of Technology (MIT), Cambridge, M,A, 02139, USA.
authors.forename: Michael F
authors.initials: MF
authors.lastname: Rubner
mesh_terms: D000818:Animals; D002412:Cations; D002448:Cell Adhesion / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004317:Doxorubicin / Q000494:pharmacology; D036681:Fluorescence Recovery After Photobleaching; D008081:Liposomes / Q000494:pharmacology* / Q000648:ultrastructure; D051379:Mice; D009000:Monocytes / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D053758:Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D010316:Particle Size
chemical_list: D002412:Cations; D008081:Liposomes; D004317:Doxorubicin
keywords: cell backpacks; doxorubicin; echogenic liposomes; multilayer films; targeted drug delivery