title: The effects of Ostertagia occidentalis somatic antigens on ovine TLR2 and TLR4 expression.
abstract: Recognition of helminth-derived pathogen associated molecular patterns (PAMPs) by pattern recognition receptors (PRRs), including toll like receptors (TLRs) is the first step towards initiating anti-helminth immune responses. Methods : Using somatic antigens of Ostertagia occidentalis, an important abomasal parasite of ruminants, the expression of ovine TLR2 and TLR4 in peripheral blood mononuclear cells (PBMCs) was analyzed by real-time quatitative reverse-transcription polymerase chain reaction (qRT-PCR). Somatic antigens of O. occidentalis were prepared to stimulate ovine PBMCs in a time and dose dependent manner. Results : A high expression of TLR2 and TLR4 was observed in PBMCs cultured with somatic antigens of the parasites specially when PBMCs were cultured with 100 µg/ml of somatic antigens and incubated for 2h. Up-regulation of TLR2 expression was more pronounced and evident in our study. Conclsusion : Somatic antigens of O. occidentalis have immunostimulatory and dominant role on peripheral immune cells. This study provide for the first time evidence of induction of TLRs in ovine PBMCs by somatic antigen of O. occidentalis.
authors.affiliation: Dept. of Pathobiology, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.
authors.forename: Hassan
authors.initials: H
authors.lastname: Borji
authors.affiliation: Dept. of Pathobiology, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.
authors.forename: Alireza
authors.initials: A
authors.lastname: Haghparast
authors.affiliation: Dept. of Pathobiology, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.
authors.forename: Nooshinmehr
authors.initials: N
authors.lastname: Soleimani
authors.affiliation: Dept. of Clinical Science, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Azizzadeh
authors.affiliation: Central Laboratories of Khorasan Razavi Veterinary Organization, Mashhad, Iran.
authors.forename: Mohammad Hossein
authors.initials: MH
authors.lastname: Nazemshirazi
keywords: Ostertagia occidentalis; Peripheral blood mononuclear cells; Toll-like receptors