title: Long non-coding RNA HOTTIP is up-regulated and associated with poor prognosis in patients with osteosarcoma.
abstract: Long non-coding RNAs (lncRNAs) have been shown to play key roles in cancer development and progression. In this study, we focused on lncRNA HOTTIP and investigated its expression pattern, clinical significance, and biological function in osteosarcoma (OS). In the present study, lncRNA HOTTIP expression in OS tissues was examined and its correlation with clinicopathological features and patient prognosis was analyzed. In vitro assays were performed to understand the biological roles of lncRNA HOTTIP in OS progression. In the study, we found that HOTTIP expression was up-regulated in OS tissues, and correlated with advanced clinical stage and distant metastasis. OS patients with high HOTTIP expression level had poorer overall survival than those with low HOTTIP expression. Multivariable Cox proportional hazards regression analysis suggested that increased HOTTIP expression was an independent prognostic factor of overall survival in OS patients. Moreover, the results of in vitro assays showed that the suppression of HOTTIP in OS cells significantly reduced cell proliferation, migration and invasion ability. Our study demonstrated that lncRNA HOTTIP play critical roles in OS progression and could represent a novel prognostic marker and potential therapeutic target in OS patients.
authors.affiliation: Department of Trauma and Orthopedics, Trauma Emergency Center, Shanghai First People's Hospital, Shanghai Jiao Tong University Shanghai 200011, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Li
authors.affiliation: Department of Trauma and Orthopedics, Trauma Emergency Center, Shanghai First People's Hospital, Shanghai Jiao Tong University Shanghai 200011, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Cao
authors.affiliation: Department of Trauma and Orthopedics, Trauma Emergency Center, Shanghai First People's Hospital, Shanghai Jiao Tong University Shanghai 200011, China.
authors.forename: Donghua
authors.initials: D
authors.lastname: Hang
authors.affiliation: Department of Trauma and Orthopedics, Trauma Emergency Center, Shanghai First People's Hospital, Shanghai Jiao Tong University Shanghai 200011, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.affiliation: Department of Trauma and Orthopedics, Trauma Emergency Center, Shanghai First People's Hospital, Shanghai Jiao Tong University Shanghai 200011, China.
authors.forename: Qiugen
authors.initials: Q
authors.lastname: Wang
mesh_terms: D000328:Adult; D001859:Bone Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology*; D018450:Disease Progression; D005260:Female; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D012516:Osteosarcoma / Q000235:genetics* / Q000401:mortality / Q000473:pathology*; D011379:Prognosis; D016016:Proportional Hazards Models; D062085:RNA, Long Noncoding / Q000096:biosynthesis*; D060888:Real-Time Polymerase Chain Reaction; D059467:Transcriptome; D015854:Up-Regulation
chemical_list: D062085:RNA, Long Noncoding
keywords: HOTTIP; Osteosarcoma; long non-coding RNAs; prognosis