title: Effect of Roux-en-Y gastric bypass-induced weight loss on the transcriptomic profiling of subcutaneous adipose tissue.
abstract: BACKGROUND
  The changes in the transcriptomic profiling of subcutaneous adipose tissue (SAT) when weight loss stabilizes after a Roux-en-Y gastric bypass (RYGB) are still largely unknown.
  
  
  OBJECTIVE
  To investigate the changes produced in SAT gene expression of morbidly obese women when their weight loss stabilizes 2 years after RYGB.
  
  
  METHODS
  University hospital.
  
  
  METHODS
  SAT biopsies of the periumbilical area were taken before and 2 years after RYGB. Gene expression levels were assessed by microarray analysis and significant differences in gene expression were validated by real-time quantitative polymerase chain reaction. The findings were also confirmed in an independent population of morbidly obese women.
  
  
  RESULTS
  Microarray analysis revealed that the overexpressed differentially expressed genes have a prominent role in the pathways involved in biosynthetic processes, especially lipid or carboxylic ones (stearoyl-Coenzyme A desaturase-1, fatty acid desaturase-1, fatty acid elongase-6, ATP citrate lyase, fatty acid synthase, lipin-1, monoacylglycerol O-acyltransferase, patatin-like phospholipase domain containing-3, phosphate cytidylyltransferase-2, cholesteryl ester transfer protein, transmembrane 7 superfamily member 2, pyruvate carboxylase, and glycogen synthase 2). Most of the underexpressed differentially expressed genes are related with immune system and inflammation processes (immune responses, response to stress, cell death, regulation of biological quality, immune effector process, the response to endogenous stimulus, and the response to other types of stimulus).
  
  
  CONCLUSIONS
  An improvement of the SAT inflammatory and immune profile and an induction of genes involved in the regulation of lipid metabolism are shown when weight loss stabilizes 2 years after RYGB. Most of the genes shown are clearly linked to obesity and other metabolic disorders.
authors.affiliation: Department of Genomic and Personalized Medicine, Center for Life Sciences, Nazarbayev University, Astana, Kazakhstan.
authors.forename: Juan José
authors.initials: JJ
authors.lastname: González-Plaza
authors.affiliation: Unidad de Gestión Clínica de Endocrinología y Nutrición, Instituto de Investigación Biomédica de Málaga (IBIMA), Hospital Regional Universitario, Málaga, Spain.
authors.forename: Carolina
authors.initials: C
authors.lastname: Gutiérrez-Repiso
authors.affiliation: Unidad de Gestión Clínica de Endocrinología y Nutrición, Instituto de Investigación Biomédica de Málaga (IBIMA), Hospital Regional Universitario, Málaga, Spain; CIBER de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM), Málaga, Spain. Electronic address: garciasara79@hotmail.com.
authors.forename: Sara
authors.initials: S
authors.lastname: García-Serrano
authors.affiliation: Unidad de Gestión Clínica de Endocrinología y Nutrición, Instituto de Investigación Biomédica de Málaga (IBIMA), Hospital Regional Universitario, Málaga, Spain; CIBER de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM), Málaga, Spain.
authors.forename: Francisca
authors.initials: F
authors.lastname: Rodriguez-Pacheco
authors.affiliation: Unidad de Gestión Clínica de Endocrinología y Nutrición, Instituto de Investigación Biomédica de Málaga (IBIMA), Hospital Clínico Virgen de la Victoria, Málaga, Spain; CIBER Fisiopatología de la Obesidad y Nutrición Asociadas (CIBEROBN), Málaga, Spain.
authors.forename: Lourdes
authors.initials: L
authors.lastname: Garrido-Sánchez
authors.affiliation: Unidad de Gestión Clínica de Endocrinología y Nutrición, Instituto de Investigación Biomédica de Málaga (IBIMA), Hospital Clínico Virgen de la Victoria, Málaga, Spain; CIBER Fisiopatología de la Obesidad y Nutrición Asociadas (CIBEROBN), Málaga, Spain.
authors.forename: Concepción
authors.initials: C
authors.lastname: Santiago-Fernández
authors.affiliation: Unidad de Gestión Clínica de Endocrinología y Nutrición, Instituto de Investigación Biomédica de Málaga (IBIMA), Hospital Regional Universitario, Málaga, Spain.
authors.forename: Juan
authors.initials: J
authors.lastname: García-Arnés
authors.affiliation: Unidad de Gestión Clínica de Cirugía General, Digestiva y Trasplantes, Instituto de Investigación Biomédica de Málaga (IBIMA), Hospital Regional Universitario, Málaga, Spain.
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Moreno-Ruiz
authors.affiliation: Unidad de Gestión Clínica de Cirugía General, Digestiva y Trasplantes, Instituto de Investigación Biomédica de Málaga (IBIMA), Hospital Regional Universitario, Málaga, Spain.
authors.forename: Alberto
authors.initials: A
authors.lastname: Rodríguez-Cañete
authors.affiliation: Unidad de Gestión Clínica de Endocrinología y Nutrición, Instituto de Investigación Biomédica de Málaga (IBIMA), Hospital Regional Universitario, Málaga, Spain; CIBER Fisiopatología de la Obesidad y Nutrición Asociadas (CIBEROBN), Málaga, Spain. Electronic address: edugf1@gmail.com.
authors.forename: Eduardo
authors.initials: E
authors.lastname: García-Fuentes
mesh_terms: D001706:Biopsy; D005260:Female; D015390:Gastric Bypass; D005786:Gene Expression Regulation; D006801:Humans; D050356:Lipid Metabolism / Q000235:genetics*; D008055:Lipids / Q000096:biosynthesis / Q000235:genetics*; D009767:Obesity, Morbid / Q000601:surgery*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D050151:Subcutaneous Fat / Q000378:metabolism* / Q000473:pathology; D014158:Transcription, Genetic; D015431:Weight Loss / Q000235:genetics*
chemical_list: D008055:Lipids; D012333:RNA, Messenger
keywords: Bariatric surgery; Expression analysis; Functional annotation; Gastric bypass; Microarray; Morbid obesity; Subcutaneous adipose tissue; Transcriptomics