title: Investigation of local anesthetic and antimycobacterial activity of Ottonia martiana Miq. (Piperaceae).
abstract: Ottonia martiana is a plant popularly known in Brazil by the use for toothache. Ethanolic extract (EE), hexane fraction (HF), dichloromethane fraction (DF) and piperovatine obtained from O. martiana were assayed in vitro and in vivo. The acute toxicity of EE was determined, and LD50 values of 164.5 and 65.0 mg/kg by the oral and intraperitoneal routes, respectively, indicated a high toxicity for EE in vivo, explaining its popular use by topical administration only. A local anesthetic-like effect of EE and its fractions was observed in experimental models using pain induction, and such effect involved an analgesic action. The antimycobacterial activity of EE, HF, DF and piperovatine was evaluated against Mycobacterium tuberculosis H37Rv ATCC 27924. EE, HF, DF, and piperovatine showed a potential antimycobacterial effect with MICs of 16.0, 62.0, 62.0 and 8.0 μg/mL, respectively. Piperovatine was more effective than the EE or the other fractions. The selectivity index (SI=IC50/MIC) values calculated for EE, HF, DF and piperovatine based on the MICs and the cytotoxicity against J774 macrophages (IC50 by MTT assay) revealed values of 6.43, 2.34, 1.5 and 9.66, respectively.
authors.affiliation: Laboratório de Inventário Florestal, Departamento de Ciências Florestais, Universidade Federal do Paraná, Curitiba, PR, Brazil.
authors.forename: Miriam M
authors.initials: MM
authors.lastname: Cunico
authors.affiliation: Laboratório de Fisiologia e Farmacologia do SNC, Departamento de Farmacologia, Universidade Federal do Paraná, Curitiba, PR, Brazil.
authors.forename: Herbert A
authors.initials: HA
authors.lastname: Trebien
authors.affiliation: Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Fábio C
authors.initials: FC
authors.lastname: Galetti
authors.affiliation: Departamento de Farmácia, Universidade Federal do Paraná, Curitiba, PR, Brazil.
authors.forename: Obdulio G
authors.initials: OG
authors.lastname: Miguel
authors.affiliation: Departamento de Farmácia, Universidade Federal do Paraná, Curitiba, PR, Brazil.
authors.forename: Marilis D
authors.initials: MD
authors.lastname: Miguel
authors.affiliation: Laboratório de Patologia Florestal, Embrapa Florestas, Colombo, PR, Brazil.
authors.forename: Celso G
authors.initials: CG
authors.lastname: Auer
authors.affiliation: Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Célio L
authors.initials: CL
authors.lastname: Silva
authors.affiliation: Laboratório de Bioquímica e Biofísica, Instituto Butantan, São Paulo, SP, Brazil.
authors.forename: Ana Olívia
authors.initials: AO
authors.lastname: de Souza
mesh_terms: D000700:Analgesics / Q000494:pharmacology* / Q000633:toxicity; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology* / Q000633:toxicity; D006168:Guinea Pigs; D007928:Lethal Dose 50; D008752:Methylene Chloride / Q000494:pharmacology* / Q000633:toxicity; D051379:Mice; D008826:Microbial Sensitivity Tests; D026321:Piperaceae / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D011817:Rabbits; D013011:Sorbic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000633:toxicity
chemical_list: D000700:Analgesics; D000900:Anti-Bacterial Agents; D010936:Plant Extracts; C038333:piperovatine; D008752:Methylene Chloride; D013011:Sorbic Acid