title: De novo centriole formation in human cells is error-prone and does not require SAS-6 self-assembly.
abstract: Vertebrate centrioles normally propagate through duplication, but in the absence of preexisting centrioles, de novo synthesis can occur. Consistently, centriole formation is thought to strictly rely on self-assembly, involving self-oligomerization of the centriolar protein SAS-6. Here, through reconstitution of de novo synthesis in human cells, we surprisingly found that normal looking centrioles capable of duplication and ciliation can arise in the absence of SAS-6 self-oligomerization. Moreover, whereas canonically duplicated centrioles always form correctly, de novo centrioles are prone to structural errors, even in the presence of SAS-6 self-oligomerization. These results indicate that centriole biogenesis does not strictly depend on SAS-6 self-assembly, and may require preexisting centrioles to ensure structural accuracy, fundamentally deviating from the current paradigm.
authors.affiliation: Institute of Biochemistry and Molecular Biology, College of Life Sciences, National Yang-Ming University, Taipei, Taiwan.
authors.forename: Won-Jing
authors.identifier: http://orcid.org/0000-0001-9733-0839
authors.initials: WJ
authors.lastname: Wang
authors.affiliation: Electron Microscopy Resource Center, The Rockefeller University, New York, United States.
authors.forename: Devrim
authors.initials: D
authors.lastname: Acehan
authors.affiliation: Institute of Biochemistry and Molecular Biology, College of Life Sciences, National Yang-Ming University, Taipei, Taiwan.
authors.forename: Chien-Han
authors.initials: CH
authors.lastname: Kao
authors.affiliation: Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan.
authors.forename: Wann-Neng
authors.initials: WN
authors.lastname: Jane
authors.affiliation: Electron Microscopy Resource Center, The Rockefeller University, New York, United States.
authors.forename: Kunihiro
authors.initials: K
authors.lastname: Uryu
authors.affiliation: Cell Biology Program, Memorial Sloan-Kettering Cancer Center, New York, United States.
authors.forename: Meng-Fu Bryan
authors.initials: MF
authors.lastname: Tsou
mesh_terms: D018797:Cell Cycle Proteins / Q000378:metabolism*; D002460:Cell Line; D002502:Centrioles / Q000378:metabolism*; D004847:Epithelial Cells / Q000502:physiology; D006801:Humans; D001678:Organelle Biogenesis; D055503:Protein Multimerization
chemical_list: D018797:Cell Cycle Proteins; C497202:SASS6 protein, human
keywords: SAS-6; cartwheel; cell biology; centriole; centrosome; cilia; human; self-assembly