title: Simultaneous Single-Molecule Force and Fluorescence Sampling of DNA Nanostructure Conformations Using Magnetic Tweezers.
abstract: We present a hybrid single-molecule technique combining magnetic tweezers and Förster resonance energy transfer (FRET) measurements. Through applying external forces to a paramagnetic sphere, we induce conformational changes in DNA nanostructures, which are detected in two output channels simultaneously. First, by tracking a magnetic bead with high spatial and temporal resolution, we observe overall DNA length changes along the force axis. Second, the measured FRET efficiency between two fluorescent probes monitors local conformational changes. The synchronized orthogonal readout in different observation channels will facilitate deciphering the complex mechanisms of biomolecular machines.
authors.affiliation: Institute for Molecular Cell Biology, University of Münster , 48149 Münster, Germany.|Institute of Experimental Physics I, Universität Leipzig , 04103 Leipzig, Germany.
authors.forename: Felix E
authors.initials: FE
authors.lastname: Kemmerich
authors.affiliation: B CUBE - Center for Molecular Bioengineering, TU Dresden , 01307 Dresden, Germany.
authors.forename: Marko
authors.initials: M
authors.lastname: Swoboda
authors.affiliation: Institute for Molecular Cell Biology, University of Münster , 48149 Münster, Germany.|Institute of Experimental Physics I, Universität Leipzig , 04103 Leipzig, Germany.
authors.forename: Dominik J
authors.initials: DJ
authors.lastname: Kauert
authors.affiliation: B CUBE - Center for Molecular Bioengineering, TU Dresden , 01307 Dresden, Germany.
authors.forename: M Svea
authors.initials: MS
authors.lastname: Grieb
authors.affiliation: B CUBE - Center for Molecular Bioengineering, TU Dresden , 01307 Dresden, Germany.
authors.forename: Steffen
authors.initials: S
authors.lastname: Hahn
authors.affiliation: B CUBE - Center for Molecular Bioengineering, TU Dresden , 01307 Dresden, Germany.|cfaed - Center for Advancing Electronics Dresden, TU Dresden , 01307 Dresden, Germany.
authors.forename: Friedrich W
authors.initials: FW
authors.lastname: Schwarz
authors.affiliation: Institute for Molecular Cell Biology, University of Münster , 48149 Münster, Germany.|Institute of Experimental Physics I, Universität Leipzig , 04103 Leipzig, Germany.
authors.forename: Ralf
authors.initials: R
authors.lastname: Seidel
authors.affiliation: B CUBE - Center for Molecular Bioengineering, TU Dresden , 01307 Dresden, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Schlierf
mesh_terms: D004247:DNA / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry; D008280:Magnetics; D049329:Nanostructures / Q000737:chemistry*; D036103:Nanotechnology; D009690:Nucleic Acid Conformation; D052898:Optical Tweezers
chemical_list: D005456:Fluorescent Dyes; D004247:DNA
keywords: FRET; Magnetic tweezers; correlated measurements; force-fluorescence microscopy; single-molecule