title: Coated Glass Slides TACAS Are Applicable to Heat-Assisted Immunostaining and In Situ Hybridization at the Electron Microscopy Level.
abstract: We performed pre-embedding electron microscopic study for visualizing the antigen and genome of severe fever with thrombocytopenia syndrome (SFTS) virus in the cytoplasm of macrophages of the human splenic red pulp, both requesting preheating treatment of sections. To pursue this, coated glass slides with unique characteristics are needed. Namely, during staining they must prevent detaching off sections, but after staining the sections must be transferred to epoxy resin. Aminopropyltriexoxysilane-coated glass slides, widely used for immunostaining, were resistant to transfer to epoxy resin. In contrast, coated glass slides designated as Thinlayer Advanced Cytology Assay System (TACAS) were suitable for this purpose. The technique is also applicable to the coated glass slide-requiring cytology practice, in which immunocytochemical evaluation is needed after cell transfer to another glass slide.
authors.affiliation: Student, the Faculty of Medical Technology, Fujita Health University School of Health Sciences , Toyoake, Aichi, Japan ; Department of Pathology, Fujita Health University School of Medicine , Toyoake, Aichi, Japan ; Present address: Laboratory Medicine, Toyota Kosei Hospital , Toyota, Aichi, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Matsui
authors.affiliation: Department of Pathology, Fujita Health University School of Medicine , Toyoake, Aichi, Japan.
authors.forename: Takanori
authors.initials: T
authors.lastname: Onouchi
authors.affiliation: Department of Pathology, Fujita Health University School of Medicine , Toyoake, Aichi, Japan.
authors.forename: Kazuya
authors.initials: K
authors.lastname: Shiogama
authors.affiliation: Department of Pathology, Fujita Health University School of Medicine , Toyoake, Aichi, Japan.
authors.forename: Yasuyoshi
authors.initials: Y
authors.lastname: Mizutani
authors.affiliation: Department of Pathology, Fujita Health University School of Medicine , Toyoake, Aichi, Japan.
authors.forename: Ken-Ichi
authors.initials: K
authors.lastname: Inada
authors.affiliation: Department of Virology, Institute of Tropical Medicine, Nagasaki University , Nagasaki, Nagasaki, Japan.
authors.forename: Fuxun
authors.initials: F
authors.lastname: Yu
authors.affiliation: Department of Virology, Institute of Tropical Medicine, Nagasaki University , Nagasaki, Nagasaki, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Hayasaka
authors.affiliation: Department of Virology, Institute of Tropical Medicine, Nagasaki University , Nagasaki, Nagasaki, Japan.
authors.forename: Koichi
authors.initials: K
authors.lastname: Morita
authors.affiliation: Department of Molecular and Environmental Pathology, Institute of Health Bioscience, the University of Tokushima Graduate School , Tokushima, Tokushima, Japan.
authors.forename: Hirohisa
authors.initials: H
authors.lastname: Ogawa
authors.affiliation: Mahara Clinic , Anan, Tokushima, Japan.
authors.forename: Fumihiko
authors.initials: F
authors.lastname: Mahara
authors.affiliation: Department of Pathology, Fujita Health University School of Medicine , Toyoake, Aichi, Japan.
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Tsutsumi
keywords: TACAS slides; electron microscopy; immunohistochemistry; in situ hybridization; severe fever with thrombocytopenia syndrome virus