title: Sodium Iodide Symporter PET and BLI Noninvasively Reveal Mesoangioblast Survival in Dystrophic Mice.
abstract: Muscular dystrophies are a heterogeneous group of myopathies, characterized by muscle weakness and degeneration, without curative treatment. Mesoangioblasts (MABs) have been proposed as a potential regenerative therapy. To improve our understanding of the in vivo behavior of MABs and the effect of different immunosuppressive therapies, like cyclosporine A or co-stimulation-adhesion blockade therapy, on cell survival noninvasive cell monitoring is required. Therefore, cells were transduced with a lentiviral vector encoding firefly luciferase (Fluc) and the human sodium iodide transporter (hNIS) to allow cell monitoring via bioluminescence imaging (BLI) and small-animal positron emission tomography (PET). Non-H2 matched mMABs were injected in the femoral artery of dystrophic mice and were clearly visible via small-animal PET and BLI. Based on noninvasive imaging data, we were able to show that co-stim was clearly superior to CsA in reducing cell rejection and this was mediated via a reduction in cytotoxic T cells and upregulation of regulatory T cells.
authors.affiliation: Department of Imaging and Pathology, Nuclear Medicine and Molecular Imaging, KU Leuven, Leuven 3000, Belgium.
authors.forename: Bryan
authors.initials: B
authors.lastname: Holvoet
authors.affiliation: Department of Development and Regeneration, Translational Cardiomyology Lab, KU Leuven, Leuven 3000, Belgium.
authors.forename: Mattia
authors.initials: M
authors.lastname: Quattrocelli
authors.affiliation: Department of Imaging and Pathology, Nuclear Medicine and Molecular Imaging, KU Leuven, Leuven 3000, Belgium.
authors.forename: Sarah
authors.initials: S
authors.lastname: Belderbos
authors.affiliation: Department of Public Health and Primary Care, Centre for Environment and Health, KU Leuven, Leuven 3000, Belgium.
authors.forename: Lore
authors.initials: L
authors.lastname: Pollaris
authors.affiliation: Department of Morphology, Biomedical Research Institute, Lab of Histology, Universiteit Hasselt, Diepenbeek 3590, Belgium.
authors.forename: Esther
authors.initials: E
authors.lastname: Wolfs
authors.affiliation: Department of Imaging and Pathology, Nuclear Medicine and Molecular Imaging, KU Leuven, Leuven 3000, Belgium.
authors.forename: Olivier
authors.initials: O
authors.lastname: Gheysens
authors.affiliation: Department of Pharmaceutical and Pharmacological Sciences, Laboratory of Molecular Virology and Gene Therapy, Leuven Viral Vector Core, KU Leuven, Leuven 3000, Belgium.
authors.forename: Rik
authors.initials: R
authors.lastname: Gijsbers
authors.affiliation: Department of Public Health and Primary Care, Centre for Environment and Health, KU Leuven, Leuven 3000, Belgium.
authors.forename: Jeroen
authors.initials: J
authors.lastname: Vanoirbeek
authors.affiliation: Department of Development and Regeneration, Stem Cell Institute Leuven, KU Leuven, Leuven 3000, Belgium.
authors.forename: Catherine M
authors.initials: CM
authors.lastname: Verfaillie
authors.affiliation: Department of Development and Regeneration, Translational Cardiomyology Lab, KU Leuven, Leuven 3000, Belgium.
authors.forename: Maurilio
authors.initials: M
authors.lastname: Sampaolesi
authors.affiliation: Department of Imaging and Pathology, Nuclear Medicine and Molecular Imaging, KU Leuven, Leuven 3000, Belgium. Electronic address: christophe.deroose@uzleuven.be.
authors.forename: Christophe M
authors.initials: CM
authors.lastname: Deroose
mesh_terms: D000818:Animals; D001808:Blood Vessels / Q000166:cytology*; D002460:Cell Line; D002470:Cell Survival; D016572:Cyclosporine / Q000627:therapeutic use; D017930:Genes, Reporter; D006801:Humans; D007166:Immunosuppressive Agents / Q000627:therapeutic use; D049409:Luciferases, Firefly / Q000032:analysis / Q000235:genetics; D008163:Luminescent Measurements; D008810:Mice, Inbred C57BL; D008819:Mice, Nude; D024510:Muscle Development; D009137:Muscular Dystrophy, Animal / Q000175:diagnosis / Q000473:pathology / Q000628:therapy*; D061848:Optical Imaging; D049268:Positron-Emission Tomography / Q000379:methods*; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology*; D027981:Symporters / Q000032:analysis* / Q000235:genetics; D014161:Transduction, Genetic; D000099209:Sodium-Iodide Symporters
chemical_list: D016572:Cyclosporine; D007166:Immunosuppressive Agents; D049409:Luciferases, Firefly; D027981:Symporters; D000099209:Sodium-Iodide Symporters