title: Nonviral Gene Therapy of the Nervous System: Electroporation.
abstract: Electroporation has been widely used to efficiently transfer foreign genes into the mammalian central nervous system (CNS), and thus plays an important role in gene therapeutic studies on some brain disorders. A lot of work concerning electroporation is focused on gene transfer into rodent brains. This technique involves an injection of nucleic acids into the brain ventricle or specific area and then applying appropriate electrical field to the injected area. Here, we briefly introduced the advantages and the basic procedures of gene transfer into the rodent brain using electroporation. Better understanding of electroporation in rodent brain may further facilitate gene therapeutic studies on brain disorders.
authors.affiliation: Beijing Institute of Basic Medical Sciences, Beijing, China. Xuefen088@126.com.
authors.forename: Xue-Feng
authors.initials: XF
authors.lastname: Ding
authors.affiliation: Beijing Institute of Basic Medical Sciences, Beijing, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Fan
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D018274:Electroporation / Q000379:methods*; D018014:Gene Transfer Techniques; D051379:Mice
keywords: Brain disorder; Electroporation; Gene therapy; Rodent brain