title: Installation of a Rigid EDTA-Like Motif into a Protein α-Helix for Paramagnetic NMR Spectroscopy with Cobalt(II) Ions.
abstract: Coupling two copies of an iminodiacetic acid-cysteine hybrid ligand to a pair of cysteine residues positioned in an i, i+4 arrangement within a protein α-helix leads to generation of an EDTA-like metal ion-binding motif. Rigid binding of a Co(II) ion by this motif produces pseudo-contact shifts suitable for paramagnetic NMR structural studies.
authors.affiliation: Monash Institute of Pharmaceutical Sciences, Monash University, 381 Royal Parade, Parkville, 3052, Victoria, Australia. james.swarbrick@monash.edu.
authors.forename: James D
authors.identifier: http://orcid.org/0000-0002-0238-7929
authors.initials: JD
authors.lastname: Swarbrick
authors.affiliation: Monash Institute of Pharmaceutical Sciences, Monash University, 381 Royal Parade, Parkville, 3052, Victoria, Australia.
authors.forename: Phuc
authors.initials: P
authors.lastname: Ung
authors.affiliation: Monash Institute of Pharmaceutical Sciences, Monash University, 381 Royal Parade, Parkville, 3052, Victoria, Australia.
authors.forename: Matthew L
authors.initials: ML
authors.lastname: Dennis
authors.affiliation: Monash Institute of Pharmaceutical Sciences, Monash University, 381 Royal Parade, Parkville, 3052, Victoria, Australia.
authors.forename: Michael D
authors.initials: MD
authors.lastname: Lee
authors.affiliation: Monash Institute of Pharmaceutical Sciences, Monash University, 381 Royal Parade, Parkville, 3052, Victoria, Australia.
authors.forename: Sandeep
authors.initials: S
authors.lastname: Chhabra
authors.affiliation: Monash Institute of Pharmaceutical Sciences, Monash University, 381 Royal Parade, Parkville, 3052, Victoria, Australia. bim.graham@monash.edu.
authors.forename: Bim
authors.initials: B
authors.lastname: Graham
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D003035:Cobalt / Q000737:chemistry*; D004492:Edetic Acid / Q000737:chemistry*; D008024:Ligands; D009682:Magnetic Resonance Spectroscopy; D011485:Protein Binding
chemical_list: D008024:Ligands; D003035:Cobalt; D004492:Edetic Acid
keywords: alpha-helix; cobalt; paramagnetic NMR spectroscopy; protein structures; pseudo-contact shifts