title: Biogenesis of Escherichia coli DMSO Reductase: A Network of Participants for Protein Folding and Complex Enzyme Maturation.
abstract: Protein folding and structure have been of interest since the dawn of protein chemistry. Following translation from the ribosome, a protein must go through various steps to become a functional member of the cellular society. Every protein has a unique function in the cell and is classified on this basis. Proteins that are involved in cellular respiration are the bioenergetic workhorses of the cell. Bacteria are resilient organisms that can survive in diverse environments by fine tuning these workhorses. One class of proteins that allow survival under anoxic conditions are anaerobic respiratory oxidoreductases, which utilize many different compounds other than oxygen as its final electron acceptor. Dimethyl sulfoxide (DMSO) is one such compound. Respiration using DMSO as a final electron acceptor is performed by DMSO reductase, converting it to dimethyl sulfide in the process. Microbial respiration using DMSO is reviewed in detail by McCrindle et al. (Adv Microb Physiol 50:147-198, 2005). In this chapter, we discuss the biogenesis of DMSO reductase as an example of the participant network for complex iron-sulfur molybdoenzyme maturation pathways.
authors.affiliation: Department of Biological Sciences, University of Calgary, BI156 Biological Sciences Bldg, 2500 University Dr NW, Calgary, AB, T2N 1N4, Canada. catchan@ucalgary.ca.
authors.forename: Catherine S
authors.initials: CS
authors.lastname: Chan
authors.affiliation: Department of Biological Sciences, University of Calgary, BI156 Biological Sciences Bldg, 2500 University Dr NW, Calgary, AB, T2N 1N4, Canada. turnerr@ucalgary.ca.
authors.forename: Raymond J
authors.initials: RJ
authors.lastname: Turner
mesh_terms: D004926:Escherichia coli / Q000201:enzymology*; D007506:Iron-Sulfur Proteins / Q000096:biosynthesis* / Q000737:chemistry; D010088:Oxidoreductases / Q000096:biosynthesis* / Q000737:chemistry; D017510:Protein Folding
chemical_list: D007506:Iron-Sulfur Proteins; D010088:Oxidoreductases; C045664:dimethyl sulfoxide reductase
keywords: Dimethyl sulfoxide (DMSO); E. coli; Reductase