title: Identification of a New Ribonucleoside Inhibitor of Ebola Virus Replication.
abstract: The current outbreak of Ebola virus (EBOV) in West Africa has claimed the lives of more than 15,000 people and highlights an urgent need for therapeutics capable of preventing virus replication. In this study we screened known nucleoside analogues for their ability to interfere with EBOV replication. Among them, the cytidine analogue β-d-N4-hydroxycytidine (NHC) demonstrated potent inhibitory activities against EBOV replication and spread at non-cytotoxic concentrations. Thus, NHC constitutes an interesting candidate for the development of a suitable drug treatment against EBOV.
authors.affiliation: Molecular Basis of Viral Pathogenicity, CIRI, INSERM, U1111-CNRS UMR5308, Université de Lyon, Université Claude Bernard Lyon 1, Ecole Normale Supérieure de Lyon, Lyon 69007, France. olivier.reynard@inserm.fr.
authors.forename: Olivier
authors.initials: O
authors.lastname: Reynard
authors.affiliation: Host Pathogen interaction during lentiviral infection, CIRI, INSERM, U1111-CNRS UMR5308, Université de Lyon, Université Claude Bernard Lyon 1, Ecole Normale Supérieure de Lyon, Lyon 69007, France. xuan-nhi.nguyen@ens-lyon.fr.
authors.forename: Xuan-Nhi
authors.initials: XN
authors.lastname: Nguyen
authors.affiliation: Molecular Basis of Viral Pathogenicity, CIRI, INSERM, U1111-CNRS UMR5308, Université de Lyon, Université Claude Bernard Lyon 1, Ecole Normale Supérieure de Lyon, Lyon 69007, France. nathalie.alazard-dany@inserm.fr.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Alazard-Dany
authors.affiliation: Host Pathogen interaction during lentiviral infection, CIRI, INSERM, U1111-CNRS UMR5308, Université de Lyon, Université Claude Bernard Lyon 1, Ecole Normale Supérieure de Lyon, Lyon 69007, France. vbarateau@ens-lyon.fr.
authors.forename: Véronique
authors.initials: V
authors.lastname: Barateau
authors.affiliation: Host Pathogen interaction during lentiviral infection, CIRI, INSERM, U1111-CNRS UMR5308, Université de Lyon, Université Claude Bernard Lyon 1, Ecole Normale Supérieure de Lyon, Lyon 69007, France. acimarel@ens-lyon.fr.
authors.forename: Andrea
authors.initials: A
authors.lastname: Cimarelli
authors.affiliation: Molecular Basis of Viral Pathogenicity, CIRI, INSERM, U1111-CNRS UMR5308, Université de Lyon, Université Claude Bernard Lyon 1, Ecole Normale Supérieure de Lyon, Lyon 69007, France. viktor.volchkov@inserm.fr.
authors.forename: Viktor E
authors.initials: VE
authors.lastname: Volchkov
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology* / Q000633:toxicity; D002522:Chlorocebus aethiops; D003562:Cytidine / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000633:toxicity; D029043:Ebolavirus / Q000187:drug effects* / Q000502:physiology*; D014709:Vero Cells; D014779:Virus Replication / Q000187:drug effects*
chemical_list: D000998:Antiviral Agents; D003562:Cytidine; C010737:N(4)-hydroxycytidine
keywords: Ebola; Filovirus; Marburg; antiviral; inhibitors; polymerase