title: Preparation and Characterization of a Novel Decellularized Fibrocartilage "Book" Scaffold for Use in Tissue Engineering.
abstract: At the tendon-to-bone insertion, there is a unique transitional structure: tendon, non-calcified fibrocartilage, calcified fibrocartilage, and bone. The reconstruction of this special graded structure after defects or damage is an important but challenging task in orthopedics. In particular, reconstruction of the fibrocartilage zone has yet to be successfully achieved. In this study, the development of a novel book-shape scaffold derived from the extracellular matrix of fibrocartilage was reported. Specifically, fibrocartilage from the pubic symphysis was obtained from rabbits and sliced into the shape of a book (dimensions: 10 mm × 3 mm × 1 mm) with 10 layers, each layer (akin to a page of a book) with a thickness of 100-μm. These fibrocartilage "book" scaffolds were decellularized using sequentially 3 freeze-thaw cycles, 0.1% Triton X-100 with 1.5 M KCl, 0.25% trypsin, and a nuclease. Histology and DNA quantification analysis confirmed substantial removal of cells from the fibrocartilage scaffolds. Furthermore, the quantities of DNA, collagen, and glycosaminoglycan in the fibrocartilage were markedly reduced following decellularization. Scanning electron microscopy confirmed that the intrinsic ultrastructure of the fibrocartilage tissue was well preserved. Therefore, the results of this study suggest that the novel "book" fibrocartilage scaffold could have potential applications in tissue engineering.
authors.affiliation: Department of Sports Medicine, Research Centre of Sports Medicine, Xiangya Hospital, Central South University, Changsha Hunan 410008, China.|Department of Rehabilitation Medicine, Peace Hospital Attached to Changzhi Medical College, Changzhi Shanxi 046000, China.
authors.forename: Liyun
authors.initials: L
authors.lastname: Guo
authors.affiliation: Department of Sports Medicine, Research Centre of Sports Medicine, Xiangya Hospital, Central South University, Changsha Hunan 410008, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Qu
authors.affiliation: Department of Sports Medicine, Research Centre of Sports Medicine, Xiangya Hospital, Central South University, Changsha Hunan 410008, China.|Department of Orthopaedics, Hospital of Wuhan Sports University, Wuhan Hubei 430079, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Zheng
authors.affiliation: Department of Spine Surgery, Xiangya Hospital, Central South University, Changsha Hunan 410008, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Department of Sports Medicine, Research Centre of Sports Medicine, Xiangya Hospital, Central South University, Changsha Hunan 410008, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Department of Sports Medicine, Research Centre of Sports Medicine, Xiangya Hospital, Central South University, Changsha Hunan 410008, China.
authors.forename: Hongbin
authors.initials: H
authors.lastname: Lu
authors.affiliation: Department of Spine Surgery, Xiangya Hospital, Central South University, Changsha Hunan 410008, China.
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Hu
mesh_terms: D000818:Animals; D051445:Fibrocartilage / Q000737:chemistry*; D011817:Rabbits; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*