title: Preparation, biodistribution and scintigraphic evaluation of (99m)Tc-lincomycin.
abstract: A complex of lincomycin was synthesized with technetium-99m. The synthesis was carried out by using SnCl2.2H2O as reducing agent and ascorbic acid as stabilizer. The effect of various parameters such as amount of ligand/reducing agent, pH value and reaction time on radio labeling process was studied. The characterization of the (99m)Tc-Lincomycin was performed by HPLC and electrophoresis Biodistribution studies were carried out by analyzing the model of bacterial infectious rats (Sprague-Dawley). The uptake of infectious lesions at different time interval was also studied by using scintigraphic technique. The complex showed effective target to non-target ratio for various inflammatory or infectious lesions. The (99m)Tc-Lincomycin effective binding to living bacteria and could be used successfully as an infection imaging agent.
authors.affiliation: Department of Chemistry, Government College University Faisalabad, Pakistan.
authors.forename: Tanveer Hussain
authors.initials: TH
authors.lastname: Bokhari
authors.affiliation: Department of Chemistry, Government College University Faisalabad, Pakistan.
authors.forename: Faheem Askari
authors.initials: FA
authors.lastname: Rizvi
authors.affiliation: Isotope Production Division, PINSTECH, Nilore, Islamabad, Pakistan.
authors.forename: Samina
authors.initials: S
authors.lastname: Roohi
authors.affiliation: Department of Bioinformatics and Biotechnology, Government College University Faisalabad, Pakistan.
authors.forename: Saira
authors.initials: S
authors.lastname: Hina
authors.affiliation: Isotope Production Division, PINSTECH, Nilore, Islamabad, Pakistan.
authors.forename: Mushtaq
authors.initials: M
authors.lastname: Ahmad
authors.affiliation: Isotope Production Division, PINSTECH, Nilore, Islamabad, Pakistan.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Khalid
authors.affiliation: National Centre of Excellence in Physical Chemistry, University of Peshawar, Peshawar, Pakistan.
authors.forename: Munawar
authors.initials: M
authors.lastname: Iqbal
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000138:chemical synthesis* / Q000493:pharmacokinetics*; D001205:Ascorbic Acid / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D004195:Disease Models, Animal; D005079:Excipients / Q000737:chemistry; D008034:Lincomycin / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000138:chemical synthesis* / Q000493:pharmacokinetics*; D008297:Male; D010084:Oxidation-Reduction; D011817:Rabbits; D011868:Radioisotopes; D011877:Radionuclide Imaging; D019275:Radiopharmaceuticals / Q000008:administration & dosage / Q000138:chemical synthesis* / Q000493:pharmacokinetics*; D017207:Rats, Sprague-Dawley; D019163:Reducing Agents / Q000737:chemistry; D013203:Staphylococcal Infections / Q000000981:diagnostic imaging* / Q000378:metabolism / Q000382:microbiology; D013211:Staphylococcus aureus / Q000472:pathogenicity; D013667:Technetium; D017971:Tin Compounds / Q000737:chemistry; D014018:Tissue Distribution
chemical_list: D000900:Anti-Bacterial Agents; D005079:Excipients; D011868:Radioisotopes; D019275:Radiopharmaceuticals; D019163:Reducing Agents; D017971:Tin Compounds; C023599:stannous chloride; D013667:Technetium; D008034:Lincomycin; D001205:Ascorbic Acid