title: Assessment of sub-chronic, hematological and histopathological toxicities of a herbal combination.
abstract: The herbal combination under study consists of Withania somnifera, Tribulus terrestris, Mucuna pruriens and Argyria speciosa. Present study is mainly designed to investigate the gross physical, sub-chronic, hematological and histopathological effects of the combination widely used for its stimulating, revitalizing and fertility boosting effects in Pakistan. Sub-chronic, hematological and histopathological outcomes of herbal combination were assessed on 27 albino rabbits weighing from 1000 gm-1500 gm after giving herbal combination for 60 days in two doses 27 and 81 mg/kg against control. No significant toxicity was revealed during the entire period of study, however some biochemical changes were observed in kidney and liver but these changes did not coincide with histopathological findings. There was no mortality and evidence of systemic toxicity including hematological toxicity following 60 days administration of herbal combination. Results of present study suggest that further studies are required on large number of animals before reaching to a definite conclusion, more over clinical studies should also be conducted to confirm the possible toxic effects of the herbal combination.
authors.affiliation: Department of Pharmacology, Faculty of Pharmacy, University of Karachi, Karachi, Pakistan.
authors.forename: Shadab
authors.initials: S
authors.lastname: Ahmed
authors.affiliation: Department of Pharmacology, Faculty of Pharmacy, University of Karachi, Karachi, Pakistan.
authors.forename: Rafeeq Alam
authors.initials: RA
authors.lastname: Khan
authors.affiliation: Department of Basic Sciences, College of Science and Health Profession, King Saud bin Abdulaziz University for Health Sciences,Riyadh, KSA.
authors.forename: Zeeshan
authors.initials: Z
authors.lastname: Feroz
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood; D001773:Blood Cells / Q000187:drug effects* / Q000378:metabolism; D004338:Drug Combinations; D005260:Female; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D028321:Plant Preparations / Q000633:toxicity*; D011817:Rabbits; D018570:Risk Assessment; D013997:Time Factors; D059550:Toxicity Tests, Subchronic
chemical_list: D015415:Biomarkers; D004338:Drug Combinations; D028321:Plant Preparations