title: Distribution of genetic variants of oxidative stress metabolism genes: Paraoxonase 1 (PON1) and Glutathione S-transferase (GSTM1/GSTT1) in a population from Southeastern Mexico.
abstract: BACKGROUND
  Paraoxonase 1 (PON1) and glutathione S-transferases (GSTs) are involved in the biotransformation of xenobiotics. Variation in the enzyme concentration and activity suggests individual differences for the degree of protection against oxidative stress.
  
  
  OBJECTIVE
  This study analysed the distribution of SNPs Q192R, L55M (PON1) and variants in GSTM1 and GSTT1 genes in a population from Southeastern Mexico.
  
  
  METHODS
  One hundred and fifty-one Mexican Mestizo healthy volunteers were included. PON1 polymorphisms were determined by Taqman allele discrimination real time-PCR, whereas GSTM1 and GSTT1 genes were determined with a multiplex PCR-based method.
  
  
  RESULTS
  All genotypes were in Hardy-Weinberg equilibrium, except for GSTM1. The genotypic distributions of Q192R and L55M were 22% QQ, 48% QR, 30% RR, 62% LL, 34% LM and 4% MM, respectively, whereas the allele frequencies were 0.46 (Q), 0.54 (R), 0.79 (L) and 0.21 (M). The most frequent haplotype was R/L (46.7%). It was found that 31% and 9% of the individuals had the GSTM1 and GSTT1 null genotype, respectively. The frequency of the combined null genotype GSTM1*0/GSTT1*0 was 4.64%.
  
  
  CONCLUSIONS
  The results showed that the frequencies of polymorphisms of PON1, GSTM1 and GSTT1 in the Yucatán population differ to those observed in other ethnic groups and provide useful data for epidemiological studies.
authors.affiliation: a Laboratorio de Genética , Centro de Investigaciones Regionales 'Dr. Hideyo Noguchi', Universidad Autónoma de Yucatán . Mérida, Yucatán , Mexico and.
authors.forename: I
authors.initials: I
authors.lastname: García-González
authors.affiliation: b Centro Estatal de la Transfusión Sanguínea, Secretaria de Salud de Yucatán , Mérida, Yucatán , Mexico.
authors.forename: R
authors.initials: R
authors.lastname: Mendoza-Alcocer
authors.affiliation: a Laboratorio de Genética , Centro de Investigaciones Regionales 'Dr. Hideyo Noguchi', Universidad Autónoma de Yucatán . Mérida, Yucatán , Mexico and.
authors.forename: G J
authors.initials: GJ
authors.lastname: Pérez-Mendoza
authors.affiliation: a Laboratorio de Genética , Centro de Investigaciones Regionales 'Dr. Hideyo Noguchi', Universidad Autónoma de Yucatán . Mérida, Yucatán , Mexico and.
authors.forename: R
authors.initials: R
authors.lastname: Rubí-Castellanos
authors.affiliation: a Laboratorio de Genética , Centro de Investigaciones Regionales 'Dr. Hideyo Noguchi', Universidad Autónoma de Yucatán . Mérida, Yucatán , Mexico and.
authors.forename: L
authors.initials: L
authors.lastname: González-Herrera
mesh_terms: D043303:Aryldialkylphosphatase / Q000235:genetics*; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D014644:Genetic Variation; D005982:Glutathione Transferase / Q000235:genetics*; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D008297:Male; D008800:Mexico; D018384:Oxidative Stress / Q000235:genetics*; D010802:Phylogeny
chemical_list: D043303:Aryldialkylphosphatase; D005982:Glutathione Transferase; C413545:glutathione S-transferase T1; C468406:PON1 protein, human
keywords: GSTs; PON1; populations