title: Compound 48/80, a Mast Cell Stimulator, Enhances Synthesis of IgE and IgG Induced by Intranasal Application of Ovalbumin in Mice.
abstract: Mast cells are well established effector cells of type I hypersensitivity reactions such as allergic rhinitis. However, recent studies have suggested that activated mast cells enhance local immunoglobulin E (IgE) synthesis in the nasal mucosa of allergic rhinitis patients. Therefore, we hypothesized that non-immunological mast cell activators may have the potential to enhance local IgE synthesis. Here, we examined the effect of compound 48/80 (C48/80), a mast cell activator, on IgE and immunoglobulin G (IgG) synthesis. Female Balb/c mice were intranasally administered a mixture of ovalbumin (OVA) (1-10 µg/nose) and C48/80 (1-100 µg/nose) on days 0, 7, 14 and 21 and on consecutive days from day 28 to day 42. Intranasal administration of C48/80 with OVA increased serum OVA-specific IgE and IgG. Double staining with fluorescent-labeled OVA and fluorescent-labeled IgE- or IgG-specific antibody demonstrated the presence of OVA-specific IgE- or IgG-producing cells in the nasal mucosa of sensitized mice. Moreover, intranasal administration of C48/80 with OVA increased the nasal mucosal interleukin (IL)-4 level and enhanced the OVA-induced symptom of sneezing. These results suggested that simultaneous activation of mast cells with antigen exposure enhances local IgE and IgG synthesis.
authors.affiliation: Department of Pharmacology, Faculty of Pharmaceutical Sciences, Tokushima Bunri University.
authors.forename: Nobuaki
authors.initials: N
authors.lastname: Matsui
authors.forename: Daisuke
authors.initials: D
authors.lastname: Ito
authors.forename: Yukari
authors.initials: Y
authors.lastname: Takabatake
authors.forename: Eri
authors.initials: E
authors.lastname: Nashioka
authors.forename: Shingo
authors.initials: S
authors.lastname: Tada
authors.forename: Masato
authors.initials: M
authors.lastname: Kanagawa
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Fukuishi
authors.forename: Masaaki
authors.initials: M
authors.lastname: Akagi
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D005260:Female; D007073:Immunoglobulin E / Q000378:metabolism*; D007074:Immunoglobulin G / Q000378:metabolism*; D015847:Interleukin-4 / Q000378:metabolism; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism; D008807:Mice, Inbred BALB C; D009092:Mucous Membrane / Q000378:metabolism; D009297:Nasal Mucosa / Q000378:metabolism*; D010047:Ovalbumin / Q000276:immunology*; D065631:Rhinitis, Allergic / Q000276:immunology* / Q000378:metabolism; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology*
chemical_list: D007074:Immunoglobulin G; D015847:Interleukin-4; D007073:Immunoglobulin E; D003189:p-Methoxy-N-methylphenethylamine; D010047:Ovalbumin