title: The expression of SEIPIN in the mouse central nervous system.
abstract: Immunohistochemical staining was used to investigate the expression pattern of SEIPIN in the mouse central nervous system. SEIPIN was found to be present in a large number of areas, including the motor and somatosensory cortex, the thalamic nuclei, the hypothalamic nuclei, the mesencephalic nuclei, some cranial motor nuclei, the reticular formation of the brainstem, and the vestibular complex. Double labeling with NeuN antibody confirmed that SEIPIN-positive cells in some nuclei were neurons. Retrograde tracer injections into the spinal cord revealed that SEIPIN-positive neurons in the motor and somatosensory cortex and other movement related nuclei project to the mouse spinal cord. The present study found more nuclei positive for SEIPIN than shown using in situ hybridization and confirmed the presence of SEIPIN in neurons projecting to the spinal cord. The results of this study help to explain the clinical manifestations of patients with Berardinelli-Seip congenital lipodystrophy (Bscl2) gene mutations.
authors.affiliation: School of Biotechnology and Biomolecular Sciences, The University of New South Wales, NSW, Australia.
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Gynecology and Obstetrics, Linyi Hospital, Linyi, Shangdong Province, China.
authors.forename: Beibei
authors.initials: B
authors.lastname: Xie
authors.affiliation: School of Biotechnology and Biomolecular Sciences, The University of New South Wales, NSW, Australia.
authors.forename: Yanfei
authors.initials: Y
authors.lastname: Qi
authors.affiliation: School of Biotechnology and Biomolecular Sciences, The University of New South Wales, NSW, Australia.
authors.forename: Ximing
authors.initials: X
authors.lastname: Du
authors.affiliation: Department of Neurology, Shanghai No. 1 People's Hospital, Shanghai, China.
authors.forename: Shaoshi
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Neurology, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.
authors.forename: Yumei
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Neuroscience Research Australia, 139 Barker Street, Randwick, NSW, 2031, Australia.|School of Medical Sciences, The University of New South Wales, Kensington, NSW, Australia.
authors.forename: George
authors.initials: G
authors.lastname: Paxinos
authors.affiliation: School of Biotechnology and Biomolecular Sciences, The University of New South Wales, NSW, Australia. h.rob.yang@unsw.edu.au.
authors.forename: Hongyuan
authors.initials: H
authors.lastname: Yang
authors.affiliation: Neuroscience Research Australia, 139 Barker Street, Randwick, NSW, 2031, Australia. h.liang@neura.edu.au.|School of Medical Sciences, The University of New South Wales, Kensington, NSW, Australia. h.liang@neura.edu.au.
authors.forename: Huazheng
authors.initials: H
authors.lastname: Liang
mesh_terms: D052436:Adipose Tissue, White / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000166:cytology* / Q000378:metabolism*; D044388:GTP-Binding Protein gamma Subunits; D020962:Heterotrimeric GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D056347:Neuroanatomical Tract-Tracing Techniques; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D013116:Spinal Cord / Q000166:cytology* / Q000378:metabolism*
chemical_list: C115783:Bscl2 protein, mouse; D044388:GTP-Binding Protein gamma Subunits; D020962:Heterotrimeric GTP-Binding Proteins
keywords: Brain; Motor control; SEIPIN; Seipinopathy; Spinal cord; Supraspinal projection