title: Derivation of Equine-Induced Pluripotent Stem Cell Lines Using a piggyBac Transposon Delivery System and Temporal Control of Transgene Expression.
abstract: The discovery of induced pluripotent stem cells (iPSCs) has had a transforming effect on our understanding of biology and has brought an enormous promise to regenerative medicine. It has opened up a magnitude of unprecedented possibilities to study disease processes in vitro, model them in animal systems, and develop patient-specific cell-based regenerative therapies. iPSCs derived from other than the human species will be instrumental for bringing these prospects to fruition by providing preclinical models and novel treatments for veterinary medicine. In this chapter, we describe the derivation of iPSCs from equine embryonic fibroblasts using a non-viral method developed in our laboratory and originally applied to the murine and human systems (Woltjen et al., Nature 458:766-770, 2009). We will detail the procedures involved and discuss potential pitfalls as well as elaborate on possible variations and future improvements of this technique.
authors.affiliation: Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, 60 Murray St., Toronto, ON, Canada, M5T 3X7.
authors.forename: Kristina
authors.initials: K
authors.lastname: Nagy
authors.affiliation: Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, 60 Murray St., Toronto, ON, Canada, M5T 3X7. nagy@mshri.on.ca.|Department of Obstetrics and Gynecology, University of Toronto, Toronto, ON, Canada. nagy@mshri.on.ca.|Lunenfeld-Tanenbaum, Research Institute, Mount Sinai Hospital, Joseph & Wolf Lebovic Health Complex, 600 University Ave., Toronto, ON, Canada, M5G 1X5. nagy@mshri.on.ca.
authors.forename: Andras
authors.initials: A
authors.lastname: Nagy
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D065150:Cellular Reprogramming; D004251:DNA Transposable Elements; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism*; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics*; D006736:Horses; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism*; D014162:Transfection; D019076:Transgenes
chemical_list: D004251:DNA Transposable Elements
keywords: Equine; Horse; Induced pluripotent stem cells; Non-viral; Reprogramming; Tetracycline-inducible transgene; iPSC; piggyBac transposon