title: Efficacy of a new bivalent vaccine of porcine circovirus type 2 and Mycoplasma hyopneumoniae (Fostera™ PCV MH) under experimental conditions.
abstract: The objective of this study was to evaluate the efficacy of a new bivalent vaccine (Fostera™ PCV MH, Zoetis) of porcine circovirus type 2 (PCV2) and Mycoplasma hyopneumoniae in growing pigs under experimental conditions. A total of 80 pigs were randomly divided into 8 groups (10 pigs per group). The pigs were administered the bivalent vaccine intramuscularly as a 2.0 mL dose at 21 days of age based on the manufacturer's instructions. Three weeks after vaccination, the pigs were inoculated with either PCV2 (intranasal route) or M. hyopneumoniae (intratracheal route) or both. Regardless of the type of inoculation, vaccinated pigs after challenge exhibited effective reduction of clinical signs, PCV2 viremia levels and mycoplasma nasal shedding, and lung and lymphoid lesion when compared to unvaccinated challenged pigs. Vaccinated challenged pigs had significantly higher (P<0.05) levels of PCV2-specific neutralizing antibodies, and numbers of PCV2-and M. hyopneumoniae-specific interferon-γ secreting cells compared to unvaccinated challenged pigs. This study demonstrates that the bivalent vaccine is able to protect pigs against either PCV2 or M. hyopneumoniae infection or both based on clinical, microbiological, immunological, and pathological evaluation.
authors.affiliation: Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 151-742, Republic of Korea.
authors.forename: Changhoon
authors.initials: C
authors.lastname: Park
authors.affiliation: Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 151-742, Republic of Korea.
authors.forename: Jiwoon
authors.initials: J
authors.lastname: Jeong
authors.affiliation: Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 151-742, Republic of Korea.
authors.forename: Kyuhyung
authors.initials: K
authors.lastname: Choi
authors.affiliation: Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 151-742, Republic of Korea. Electronic address: swine@snu.ac.kr.
authors.forename: Chanhee
authors.initials: C
authors.lastname: Chae
mesh_terms: D032761:Animal Experimentation; D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood; D057138:Bacterial Shedding; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000276:immunology*; D018173:Circoviridae Infections / Q000517:prevention & control / Q000662:veterinary*; D017925:Circovirus / Q000276:immunology*; D007273:Injections, Intramuscular; D007371:Interferon-gamma / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008168:Lung / Q000473:pathology; D008198:Lymph Nodes / Q000473:pathology; D009175:Mycoplasma Infections / Q000517:prevention & control / Q000662:veterinary*; D045705:Mycoplasma hyopneumoniae / Q000276:immunology*; D011897:Random Allocation; D013552:Swine; D013553:Swine Diseases / Q000517:prevention & control*; D016896:Treatment Outcome; D017778:Vaccines, Combined / Q000008:administration & dosage / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology*; D014766:Viremia
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D001428:Bacterial Vaccines; D017778:Vaccines, Combined; D014765:Viral Vaccines; D007371:Interferon-gamma
keywords: Bivalent vaccine; Mycoplasma hyopneumoniae; Porcine circovirus type 2; Porcine circovirus-associated disease; Porcine respiratory disease complex