title: Comprehensive studies on the nature of interaction between carboxylated multi-walled carbon nanotubes and bovine serum albumin.
abstract: Herein, the interaction between carboxylated multi-walled carbon nanotubes (MWCNTs-COOH) and bovine serum albumin has been investigated by using circular dichroism, UV-vis, and fluorescence spectroscopic methods and molecular modeling in order to better understand the basic behavior of carbon nanotubes in biological systems. The spectral results showed that MWCNTs-COOH bound to BSA and induced the relatively large changes in secondary structure of protein by mainly hydrophobic forces and π-π stacking interactions. Thermal denaturation of BSA in the presence of MWCNTs-COOH indicated that carbon nanotubes acted as a structure destabilizer for BSA. In addition, the putative binding site of MWCNTs-COOH on BSA was near to domain II. With regard to human health, the present study could provide a better understanding of the biological properties, cytotocicity of surface modified carbon nanotubes.
authors.affiliation: Chemistry and Chemical Engineering, Nanjing University of Technology, Nanjing City, Jiangsu Province, 210009, People's Republic of China; Institute of Applied Chemistry and Environmental Engineering, Yancheng Teachers University, Yancheng City, Jiangsu Province, 224002, People's Republic of China.
authors.forename: Kai
authors.initials: K
authors.lastname: Lou
authors.affiliation: Institute of Applied Chemistry and Environmental Engineering, Yancheng Teachers University, Yancheng City, Jiangsu Province, 224002, People's Republic of China.
authors.forename: Zhaohua
authors.initials: Z
authors.lastname: Zhu
authors.affiliation: Institute of Applied Chemistry and Environmental Engineering, Yancheng Teachers University, Yancheng City, Jiangsu Province, 224002, People's Republic of China.
authors.forename: Hongmei
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Institute of Applied Chemistry and Environmental Engineering, Yancheng Teachers University, Yancheng City, Jiangsu Province, 224002, People's Republic of China. Electronic address: wyqing76@126.com.
authors.forename: Yanqing
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Institute of Applied Chemistry and Environmental Engineering, Yancheng Teachers University, Yancheng City, Jiangsu Province, 224002, People's Republic of China.
authors.forename: Xiaojiong
authors.initials: X
authors.lastname: Wang
authors.affiliation: Institute of Applied Chemistry and Environmental Engineering, Yancheng Teachers University, Yancheng City, Jiangsu Province, 224002, People's Republic of China. Electronic address: yctu_caojian@126.com.
authors.forename: Jian
authors.initials: J
authors.lastname: Cao
mesh_terms: D000327:Adsorption; D000818:Animals; D002417:Cattle; D057927:Hydrophobic and Hydrophilic Interactions; D008958:Models, Molecular; D037742:Nanotubes, Carbon / Q000737:chemistry*; D011485:Protein Binding; D011487:Protein Conformation; D011489:Protein Denaturation; D055550:Protein Stability; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000378:metabolism
chemical_list: D037742:Nanotubes, Carbon; D012710:Serum Albumin, Bovine
keywords: Adsorption; Binding interaction; Bovine serum albumin; Carboxylated multi-walled carbon nanotubes