title: Quantitative Shotgun Proteomics Analysis of Rice Anther Proteins after Exposure to High Temperature.
abstract: In rice, the stage of development most sensitive to high temperature stress is flowering, and exposure at this stage can result in spikelet sterility, thereby leading to significant yield losses. In this study, protein expression patterns of rice anthers from Dianxi4, a high temperature tolerant Japonica rice variety, were compared between samples exposed to high temperature and those grown in natural field conditions in Korea. Shotgun proteomics analysis of three replicate control and high-temperature-treated samples identified 3,266 nonredundant rice anther proteins (false discovery rate < 0.01). We found that high levels of ATP synthase, cupin domain-containing proteins, and pollen allergen proteins were present in rice anthers. Comparative analyses of 1,944 reproducibly expressed proteins identified 139 differentially expressed proteins, with 95 increased and 44 decreased in response to high temperature conditions. Heat shock, DnaK family, and chaperone proteins showed highly increased expression, suggesting that the high temperature tolerance of Dianxi4 is achieved by stabilization of proteins in pollen cells. Trehalose synthase was also highly increased after heat treatment, suggesting a possible role for trehalose in preventing protein denaturation through desiccation.
authors.affiliation: Department of Applied Bioscience, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Mijeong
authors.initials: M
authors.lastname: Kim
authors.affiliation: Department of Applied Bioscience, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Hijin
authors.initials: H
authors.lastname: Kim
authors.affiliation: Department of Applied Bioscience, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Wondo
authors.initials: W
authors.lastname: Lee
authors.affiliation: Department of Applied Bioscience, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Yoonjung
authors.initials: Y
authors.lastname: Lee
authors.affiliation: Department of Plant Bioscience, Pusan National University, Milyang 627-706, Republic of Korea.
authors.forename: Soon-Wook
authors.initials: SW
authors.lastname: Kwon
authors.affiliation: Department of Applied Bioscience, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Joohyun
authors.initials: J
authors.lastname: Lee