title: The kin17 Protein in Murine Melanoma Cells.
abstract: kin17 has been described as a protein involved in the processes of DNA replication initiation, DNA recombination, and DNA repair. kin17 has been studied as a potential molecular marker of breast cancer. This work reports the detection and localization of this protein in the murine melanoma cell line B16F10-Nex2 and in two derived subclones with different metastatic potential, B16-8HR and B16-10CR. Nuclear and chromatin-associated protein fractions were analyzed, and kin17 was detected in all fractions, with an elevated concentration observed in the chromatin-associated fraction of the clone with low metastatic potential, suggesting that the kin17 expression level could be a marker of melanoma.
authors.affiliation: Departamento de Biotecnologia, Genética e Biologia Celular, Universidade Estadual de Maringá, 87020-900 Maringá, Paraná, Brazil. anelise.andre@gmail.com.
authors.forename: Anelise C
authors.initials: AC
authors.lastname: Ramos
authors.affiliation: Departamento de Biotecnologia, Genética e Biologia Celular, Universidade Estadual de Maringá, 87020-900 Maringá, Paraná, Brazil. vapigas@gmail.com.
authors.forename: Vanessa P
authors.initials: VP
authors.lastname: Gaspar
authors.affiliation: Departamento de Biotecnologia, Genética e Biologia Celular, Universidade Estadual de Maringá, 87020-900 Maringá, Paraná, Brazil. sabrinamg12@hotmail.com.
authors.forename: Sabrina M G
authors.initials: SM
authors.lastname: Kelmer
authors.affiliation: Laboratório de Imunobiologia do Câncer, Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de São Paulo, 04023-062 São Paulo, São Paulo, Brazil. tarcis.sellani@gmail.com.
authors.forename: Tarciso A
authors.initials: TA
authors.lastname: Sellani
authors.affiliation: Laboratório de Imunobiologia do Câncer, Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de São Paulo, 04023-062 São Paulo, São Paulo, Brazil. anagurbanin@gmail.com.
authors.forename: Ana G U
authors.initials: AG
authors.lastname: Batista
authors.affiliation: Departamento de Biotecnologia, Genética e Biologia Celular, Universidade Estadual de Maringá, 87020-900 Maringá, Paraná, Brazil. qalneto@gmail.com.
authors.forename: Quirino A
authors.initials: QA
authors.lastname: De Lima Neto
authors.affiliation: Laboratório de Imunobiologia do Câncer, Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de São Paulo, 04023-062 São Paulo, São Paulo, Brazil. rodrigues.elaine@unifesp.br.
authors.forename: Elaine G
authors.initials: EG
authors.lastname: Rodrigues
authors.affiliation: Departamento de Biotecnologia, Genética e Biologia Celular, Universidade Estadual de Maringá, 87020-900 Maringá, Paraná, Brazil. mafernandez@uem.br.
authors.forename: Maria A
authors.initials: MA
authors.lastname: Fernandez
mesh_terms: D000818:Animals; D002465:Cell Movement; D002843:Chromatin / Q000378:metabolism; D004260:DNA Repair; D004261:DNA Replication; D004268:DNA-Binding Proteins / Q000378:metabolism*; D064596:Isografts; D008545:Melanoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D008546:Melanoma, Experimental; D051379:Mice; D009362:Neoplasm Metastasis; D009687:Nuclear Proteins / Q000378:metabolism*
chemical_list: D002843:Chromatin; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; C071214:Kin protein, mouse
keywords: DNA metabolism; kin17 protein; melanoma cells