title: Topiramate effects lipolysis in 3T3-L1 adipocytes.
abstract: Studies have shown that topiramate (TPM)-induced weight loss can be dependent on the central nervous system (CNS). However, the direct action of TPM on adipose tissue has not been tested previously. Thus, the present study aimed to examine whether TPM modulates lipolysis in 3T3-L1. The 3T3-L1 cells were incubated in 50 µM TPM for 30 min. The β-adrenergic stimulator, isoproterenol, was used as a positive control. The release of lactate dehydrogenase, non-esterified fatty acid, glycerol and incorporation of14C-palmitate to lipid were analyzed. The phosphorylation of protein kinase A (PKA), hormone-sensitive lipase (HSL), adipocyte triglyceride lipase (ATGL) and perilipin A, as well as the protein levels of comparative genetic identification 58 (CGI-58) were assessed. The levels of glycerol and non-esterified fatty acid increased markedly when the cells were treated with TPM. The TPM effects were similar to the isoproterenol positive control. Additionally, TPM reduced lipogenesis. These results were observed without any change in cell viability. Finally, the phosphorylation of PKA, HSL, ATGL and perilipin A, as well as the protein levels of CGI-58 were increased compared to the control cells. These results were similar to those observed in the cells treated with isoproterenol. The present results show that TPM increased the phosphorylation of pivotal lipolytic enzymes, which induced lipolysis in 3T3-L1 adipocytes, suggesting that this drug may act directly in the adipose tissue independent from its effect on the CNS.
authors.affiliation: Laboratory of Exercise Biochemistry and Physiology, Health Sciences Unit, University of Extremo Sul Catarinense, Criciúma, SC 88806-000, Brazil.
authors.forename: Gabriela Poltronieri Campagnaro
authors.initials: GP
authors.lastname: Martins
authors.affiliation: Immunometabolism Research Group, Institute of Biomedical Sciences, University of Sao Paulo, São Paulo, SP 05508-900, Brazil.
authors.forename: Camila Oliveira
authors.initials: CO
authors.lastname: Souza
authors.affiliation: Laboratory of Exercise Biochemistry and Physiology, Health Sciences Unit, University of Extremo Sul Catarinense, Criciúma, SC 88806-000, Brazil.
authors.forename: Scherolin
authors.initials: S
authors.lastname: Marques
authors.affiliation: Laboratory of Exercise Biochemistry and Physiology, Health Sciences Unit, University of Extremo Sul Catarinense, Criciúma, SC 88806-000, Brazil.
authors.forename: Thais Fernandes
authors.initials: TF
authors.lastname: Luciano
authors.affiliation: Laboratory of Exercise Biochemistry and Physiology, Health Sciences Unit, University of Extremo Sul Catarinense, Criciúma, SC 88806-000, Brazil.
authors.forename: Bruno Luiz
authors.initials: BL
authors.lastname: DA Silva Pieri
authors.affiliation: Immunometabolism Research Group, Institute of Biomedical Sciences, University of Sao Paulo, São Paulo, SP 05508-900, Brazil.
authors.forename: José César
authors.initials: JC
authors.lastname: Rosa
authors.affiliation: School of Physical Education and Sport of Ribeirão Preto, University of São Paulo (USP), Ribeirão Preto, SP 14040-907, Brazil.
authors.forename: Adelino Sanchez Ramos
authors.initials: AS
authors.lastname: DA Silva
authors.affiliation: School of Applied Sciences, University of Campinas (UNICAMP), Limeira, SP 13484-350, Brazil.
authors.forename: José Rodrigo
authors.initials: JR
authors.lastname: Pauli
authors.affiliation: School of Applied Sciences, University of Campinas (UNICAMP), Limeira, SP 13484-350, Brazil.
authors.forename: Dennys Esper
authors.initials: DE
authors.lastname: Cintra
authors.affiliation: School of Applied Sciences, University of Campinas (UNICAMP), Limeira, SP 13484-350, Brazil.
authors.forename: Eduardo Rochete
authors.initials: ER
authors.lastname: Ropelle
authors.affiliation: Laboratory of Human Movement, São Judas Tadeu University, São Paulo, SP 03166-000, Brazil.
authors.forename: Bruno
authors.initials: B
authors.lastname: Rodrigues
authors.affiliation: Immunometabolism Research Group, Department of Physical Education, University Estadual Paulista (UNESP), Presidente Prudente, SP 19060-900, Brazil.
authors.forename: Fabio Santos
authors.initials: FS
authors.lastname: DE Lira
authors.affiliation: Laboratory of Exercise Biochemistry and Physiology, Health Sciences Unit, University of Extremo Sul Catarinense, Criciúma, SC 88806-000, Brazil.
authors.forename: Claudio Teodoro
authors.initials: CT
authors.lastname: DE Souza
keywords: 3T3-L1 adipocytes; lipolysis; obesity; topiramate; weight loss