title: Expression of NF-κB and PTEN in primary epithelial ovarian carcinoma and the correlation with chemoresistance.
abstract: The present study aims to investigate the relationship of NF-κB p65 and PTEN protein with chemotherapy resistance in ovarian cancer by measuring their expression in primary epithelial ovarian cancer, and to explore the correlation of the expression of these two proteins with ovarian carcinoma and their clinical significance. Ovarian cancer patients (n = 161) were divided into two groups: sensitive group (n = 82) and resistant group (n = 79). Expression of NF-κB p65 and PTEN protein in the ovarian cancer tissues was determined using immunohistochemistry to assess the relationship and correlation between the expression levels of these two proteins and chemotherapy resistance of ovarian carcinoma. The Cox model was used to analyze the independent risk factors associated with ovarian cancer prognosis. The expression of NF-κB p65 in the sensitive group (68.29%) was lower than that of the resistant group (94.94%). In contrast, the expression of PTEN protein in the sensitive group (50.00%) was higher than that of the resistant group (17.72%). Expression of NF-κB p65 was negatively correlated with that of PTEN protein in ovarian cancer tissue (rs = -0.246, P = 0.002). Expression of NF-κB p65 or PTEN protein and surgical stage of ovarian cancer were independent risk factors associated with chemoresistance (all P < 0.05). Low expression of PTEN and high expression of NF-κB are significant risk factors for chemotherapy resistance of ovarian cancer patients.
authors.affiliation: Department of Obstetrics and Gynecology, Shengjing Hospital Affiliated to China Medical University Shenyang 110004, P. R. China.
authors.forename: Lili
authors.initials: L
authors.lastname: Wang
authors.affiliation: Changchun Institute of Applied Chemistry, Chinese Academy of Sciences Changchun 130000, P. R. China ; University of Chinese Academy of Sciences Beijing 100000, P. R. China.
authors.forename: Chenxu
authors.initials: C
authors.lastname: Wang
authors.affiliation: Shenyang Women's and Children's Hospital Shenyang 110011, P. R. China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Jin
authors.affiliation: Chengde Medical University Affiliated Hospital Chengde 067000, P. R. China.
authors.forename: Donghui
authors.initials: D
authors.lastname: Qu
authors.affiliation: Department of Obstetrics and Gynecology, Shengjing Hospital Affiliated to China Medical University Shenyang 110004, P. R. China.
authors.forename: Huanchun
authors.initials: H
authors.lastname: Ying
mesh_terms: D000328:Adult; D000368:Aged; D000077216:Carcinoma, Ovarian Epithelial; D019008:Drug Resistance, Neoplasm / Q000502:physiology*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D009375:Neoplasms, Glandular and Epithelial / Q000378:metabolism*; D010051:Ovarian Neoplasms / Q000378:metabolism*; D051059:PTEN Phosphohydrolase / Q000378:metabolism*; D012307:Risk Factors; D051996:Transcription Factor RelA / Q000378:metabolism*; D055815:Young Adult
chemical_list: C498143:RELA protein, human; D051996:Transcription Factor RelA; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human
keywords: NF-κB; PTEN; chemoresistance; immunohistochemistry; ovarian epithelial cancer