title: Crystallization behaviour of glyceraldehyde dehydrogenase from Thermoplasma acidophilum.
abstract: The glyceraldehyde dehydrogenase from Thermoplasma acidophilum (TaAlDH) is a microbial enzyme that catalyzes the oxidation of D-glyceraldehyde to D-glycerate in the artificial enzyme cascade designed for the conversion of glucose to the organic solvents isobutanol and ethanol. Various mutants of TaAlDH were constructed by a random approach followed by site-directed and saturation mutagenesis in order to improve the properties of the enzyme that are essential for its functioning within the cascade. Two enzyme variants, wild-type TaAlDH (TaAlDHwt) and an F34M+S405N variant (TaAlDH F34M+S405N), were successfully crystallized. Crystals of TaAlDHwt belonged to the monoclinic space group P1211 with eight molecules per asymmetric unit and diffracted to a resolution of 1.95 Å. TaAlDH F34M+S405N crystallized in two different space groups: triclinic P1 with 16 molecules per asymmetric unit and monoclinic C121 with four molecules per asymmetric unit. These crystals diffracted to resolutions of 2.14 and 2.10 Å for the P1 and C121 crystals, respectively.
authors.affiliation: Faculty of Science, University of South Bohemia in Ceske Budejovice, Branisovska 31, 37005 Ceske Budejovice, Czech Republic.
authors.forename: Iuliia
authors.initials: I
authors.lastname: Iermak
authors.affiliation: Faculty of Science, University of South Bohemia in Ceske Budejovice, Branisovska 31, 37005 Ceske Budejovice, Czech Republic.
authors.forename: Oksana
authors.initials: O
authors.lastname: Degtjarik
authors.affiliation: Bio, Electro- and Chemocatalysis BioCat, Straubing Branch of the Fraunhofer Institute for Interfacial Engineering and Biotechnology IGB, Schulgasse 11a, 94315 Straubing, Germany.
authors.forename: Fabian
authors.initials: F
authors.lastname: Steffler
authors.affiliation: Chemistry of Biogenic Resources, Straubing Centre of Science, Technische Universität München, Schulgasse 16, 94315 Straubing, Germany.
authors.forename: Volker
authors.initials: V
authors.lastname: Sieber
authors.affiliation: Faculty of Science, University of South Bohemia in Ceske Budejovice, Branisovska 31, 37005 Ceske Budejovice, Czech Republic.
authors.forename: Ivana
authors.initials: I
authors.lastname: Kuta Smatanova
mesh_terms: D000595:Amino Acid Sequence; D003460:Crystallization; D008969:Molecular Sequence Data; D013401:Sugar Alcohol Dehydrogenases / Q000737:chemistry*; D013822:Thermoplasma / Q000201:enzymology*; D014961:X-Ray Diffraction
chemical_list: D013401:Sugar Alcohol Dehydrogenases; C028041:glyceraldehyde reductase
keywords: TaAlDH; Thermoplasma acidophilum; bioproduction; cell-free enzyme cascade; glyceraldehyde dehydrogenase