title: Polymeric Cryogel-Based Boronate Affinity Chromatography for Separation of Ribonucleic Acid from Bacterial Extracts.
abstract: Three-dimensional monolithic columns are preferred stationary phase in column chromatography. Conventional columns based on silica or particles are efficient in bioseparation though associated with limitations of nonspecific interaction and uneven porosity that causes high mass transfer resistance for the movement of big molecules. Cryogels as a monolith column have shown promising application in bioseparation. Cryogels column can be synthesized in the form of a monolith at sub-zero temperature through gelation of pre-synthesized polymers or polymerization of monomers. Cryogels are macroporous and mechanically stable materials. They have open interconnected micron-sized pores with a wide range of porosity (10-200 μm). Current protocol demonstrated the ability of poly(hydroxymethyl methacrylate)-co-vinylphenyl boronic acid p(HEMA-co-VPBA) cryogel matrix for selective separation of RNA from the bacterial crude extract.
authors.affiliation: Department of Chemical Engineering, Texas Tech University, Lubbock, Texas.
authors.forename: Akhilesh Kumar
authors.initials: AK
authors.lastname: Shakya
authors.affiliation: Network of Excellence for Functional Biomaterials, National University of Ireland, Galway, Republic of Ireland.
authors.forename: Akshay
authors.initials: A
authors.lastname: Srivastava
authors.affiliation: Department of Biological Sciences and Bioengineering, Indian Institute of Technology Kanpur, Kanpur (U.P), India.
authors.forename: Ashok
authors.initials: A
authors.lastname: Kumar
mesh_terms: D001419:Bacteria / Q000737:chemistry*; D001897:Boronic Acids / Q000737:chemistry*; D002846:Chromatography, Affinity / Q000379:methods*; D059985:Cryogels / Q000138:chemical synthesis / Q000737:chemistry; D004269:DNA, Bacterial / Q000302:isolation & purification*; D012329:RNA, Bacterial / Q000302:isolation & purification*
chemical_list: D001897:Boronic Acids; D059985:Cryogels; D004269:DNA, Bacterial; D012329:RNA, Bacterial
keywords: RNA separation; boronate-affinity chromatography; cryogel; monolithic column