title: MicroRNA-137 inhibits cell migration and invasion by targeting bone morphogenetic protein-7 (BMP7) in non-small cell lung cancer cells.
abstract: MicroRNA-137 (miR-137) was reported to be dysregulated in several human cancers. However, the function and mechanism of miR-137 in non-small cell lung cancer (NSCLC) is still unclear. In the current study, we explored the role of miR-137 in NSCLC progression. Using qRT-PCR, our data showed that miR-137 was significantly down-regulated in NSCLC tissues and cell lines. In vitro functional assay, we found that over-expression of miR-137 suppressed NSCLC cells proliferation, migration and invasion, indicating that miR-137 could act as a tumor suppressor in NSCLC progression. In addition, bone morphogenetic protein-7 (BMP7) was identified as a target of miR-137 in NSCLC cells, Luciferase reporter assay suggested that miR-137 directly targeted 3'-UTR of BMP7, and correlation analysis revealed that BMP7 inversely correlated with miR-137 in NSCLC tissues. Furthermore, Restoration of BMP7 remarkably reversed the tumor suppressive effects of miR-137 on NSCLC cell proliferation, migration, and invasion. Taken together, our findings suggested that miR-137/BMP7 axis could contribute to the progression of NSCLC, suggesting miR-137 as a potential therapeutic target for the treatment of NSCLC.
authors.affiliation: Department of Respiration, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang Medical University Weihui 453100, China.
authors.forename: Yan-Rong
authors.initials: YR
authors.lastname: Yang
authors.affiliation: Department of Respiration, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang Medical University Weihui 453100, China.
authors.forename: Yun-Xia
authors.initials: YX
authors.lastname: Li
authors.affiliation: Department of Respiration, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang Medical University Weihui 453100, China.
authors.forename: Xin-Yuan
authors.initials: XY
authors.lastname: Gao
authors.affiliation: Department of Respiration, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang Medical University Weihui 453100, China.
authors.forename: Sha-Sha
authors.initials: SS
authors.lastname: Zhao
authors.affiliation: Department of Respiration, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang Medical University Weihui 453100, China.
authors.forename: Shu-Zhi
authors.initials: SZ
authors.lastname: Zang
authors.affiliation: Department of Respiration, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang Medical University Weihui 453100, China.
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Zhang
mesh_terms: D020413:3' Untranslated Regions; D001665:Binding Sites; D055419:Bone Morphogenetic Protein 7 / Q000235:genetics / Q000378:metabolism*; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D057809:HEK293 Cells; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D015398:Signal Transduction; D013997:Time Factors; D014162:Transfection
chemical_list: D020413:3' Untranslated Regions; C525819:BMP7 protein, human; D055419:Bone Morphogenetic Protein 7; C545567:MIRN137 microRNA, human; D035683:MicroRNAs
keywords: Non-small cell lung cancer; bone morphogenetic protein-7; miR-137