title: Enrichment and separation of chlorogenic acid from the extract of Eupatorium adenophorum Spreng by macroporous resin.
abstract: A simple and efficient chromatographic method for separation of chlorogenic acid from Eupatorium adenophorum Spreng extract was developed. The adsorption properties of nine macroporous resins were evaluated. NKA-II resin showed much better adsorption/desorption properties. The adsorption of chlorogenic acid on NKA-II resin at 25°C was well fitted to Langmuir isotherm model and pseudo-second-order kinetic model. The dynamic adsorption and desorption experiments were carried out on columns packed with NKA-II resin to optimize the separation process. The content of chlorogenic acid in the product increased to 22.17%, with a recovery yield of 82.41%.
authors.affiliation: National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China; University of Chinese Academy of Sciences, Beijing 100039, PR China.
authors.forename: Boyan
authors.initials: B
authors.lastname: Liu
authors.affiliation: National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China; University of Chinese Academy of Sciences, Beijing 100039, PR China.
authors.forename: Beitao
authors.initials: B
authors.lastname: Dong
authors.affiliation: National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China. Electronic address: xfyuan@ipe.ac.cn.
authors.forename: Xiaofan
authors.initials: X
authors.lastname: Yuan
authors.affiliation: National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China.
authors.forename: Qirong
authors.initials: Q
authors.lastname: Kuang
authors.affiliation: National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China.
authors.forename: Qingsheng
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: Panzhihua Xiyu Biotech Co., Ltd., Panzhihua 617400, PR China.
authors.forename: Mei
authors.initials: M
authors.lastname: Yang
authors.affiliation: Panzhihua Xiyu Biotech Co., Ltd., Panzhihua 617400, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
authors.affiliation: National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China. Electronic address: bzhao@ipe.ac.cn.
authors.forename: Bing
authors.initials: B
authors.lastname: Zhao
mesh_terms: D000327:Adsorption; D036603:Ageratina / Q000737:chemistry*; D002726:Chlorogenic Acid / Q000302:isolation & purification*; D007700:Kinetics; D010936:Plant Extracts / Q000737:chemistry*; D013816:Thermodynamics
chemical_list: D010936:Plant Extracts; D002726:Chlorogenic Acid
keywords: Chlorogenic acid; Eupatorium adenophorum Spreng; NKA-II macroporous resin; Separation