title: Guillain Barré syndrome (GBS): new insights in the molecular mimicry between C. jejuni and human peripheral nerve (HPN) proteins.
abstract: Profile and immunoreactivity of proteins from HPN tissue, and from Campylobacter jejuni (O:19) were investigated. Proteins were extracted, separated by SDS-PAGE, their cross reactivity monitored by Western blotting, and identified by nHPLC-nESI-HRMS analysis. Proteins from C. jejuni, at Mw ~70 KDa were chaperone/co-chaperone proteins (GroEL, DnaK and HtpG). In the corresponding HPN band were serum albumin, neurofilament light peptide, cytoskeletal keratins and one HSP 70 and one HSP60. These chaperones reciprocally share high primary sequence homology and conservation of their known epitopes. These findings suggest that HSP chaperones may be suitable candidates involved in the molecular mimicry triggering GBS.
authors.affiliation: Faculty of Pharmacy, University Ss. Cyril and Methodius, Skopje, Macedonia; Department of Pharmacy, Faculty of Medicine, University Hasan Prishtina, Pristina, Kosovo.
authors.forename: Aida
authors.initials: A
authors.lastname: Loshaj-Shala
authors.affiliation: Department of Pharmaceutical Sciences DISFARM, University of Milan, Milan, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Regazzoni
authors.affiliation: Department of Pharmacy, Faculty of Medicine, University Hasan Prishtina, Pristina, Kosovo.
authors.forename: Armond
authors.initials: A
authors.lastname: Daci
authors.affiliation: Department of Pharmaceutical Sciences DISFARM, University of Milan, Milan, Italy.
authors.forename: Marica
authors.initials: M
authors.lastname: Orioli
authors.affiliation: Faculty of Pharmacy, University Ss. Cyril and Methodius, Skopje, Macedonia.
authors.forename: Katerina
authors.initials: K
authors.lastname: Brezovska
authors.affiliation: Faculty of Pharmacy, University Ss. Cyril and Methodius, Skopje, Macedonia.
authors.forename: Ana Poceva
authors.initials: AP
authors.lastname: Panovska
authors.affiliation: Department of Pharmaceutical Sciences DISFARM, University of Milan, Milan, Italy. Electronic address: giangiacomo.beretta@unimi.it.
authors.forename: Giangiacomo
authors.initials: G
authors.lastname: Beretta
authors.affiliation: Faculty of Pharmacy, University Ss. Cyril and Methodius, Skopje, Macedonia.
authors.forename: Ljubica
authors.initials: L
authors.lastname: Suturkova
mesh_terms: D016123:Campylobacter jejuni / Q000276:immunology / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D003429:Cross Reactions; D000939:Epitopes; D020275:Guillain-Barre Syndrome / Q000150:complications / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007633:Keratins / Q000378:metabolism*; D018832:Molecular Chaperones / Q000378:metabolism*; D018716:Molecular Mimicry; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000378:metabolism; D010525:Peripheral Nerves / Q000378:metabolism*; D016415:Sequence Alignment; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
chemical_list: D000939:Epitopes; D018832:Molecular Chaperones; D009419:Nerve Tissue Proteins; D007633:Keratins
keywords: Campylobacter jejuni; Guillain–Barré syndrome; HPLC-HR-MS/MS; Heat shock proteins; Proteomics