title: Occurrence and characterization of inducible clindamycin resistance in canine methicillin-resistant Staphylococcus pseudintermedius.
abstract: This study aimed to detect inducible clindamycin (iCLI) resistance in Staphylococcus pseudintermedius isolated from dogs in Thailand using D-zone testing. Strains that were iCLI-resistant were characterized by molecular typing and antibiogram and were detected in 10/200 S. pseudintermedius isolates (5%) from 7/41 dogs (17%). All were methicillin-resistant S. pseudintermedius (MRSP) and demonstrated multidrug resistance. The iCLI-resistant MRSP contained erm(B) and had identical or closely related DNA fingerprint patterns by pulsed-field gel electrophoresis. All iCLI-resistant MRSP strains belonged to the same clonal complex 112 (sequence types 111 and 112) by multilocus sequence typing. To avoid misinterpretation of clindamycin susceptibility, D-zone testing is recommended to promote rational antimicrobial selection and limit the clonal expansion of multidrug resistant bacteria.
authors.affiliation: Department of Veterinary Microbiology, Faculty of Veterinary Science, Chulalongkorn University, Henri-Dunant Road, Pathumwan District, Bangkok 10330, Thailand. Electronic address: pattrarat.c@chula.ac.th.
authors.forename: Pattrarat
authors.initials: P
authors.lastname: Chanchaithong
authors.affiliation: Department of Veterinary Microbiology, Faculty of Veterinary Science, Chulalongkorn University, Henri-Dunant Road, Pathumwan District, Bangkok 10330, Thailand.
authors.forename: Nuvee
authors.initials: N
authors.lastname: Prapasarakul
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002981:Clindamycin / Q000494:pharmacology*; D016172:DNA Fingerprinting / Q000662:veterinary; D004283:Dog Diseases / Q000453:epidemiology* / Q000382:microbiology; D004285:Dogs; D024901:Drug Resistance, Multiple, Bacterial; D016521:Electrophoresis, Gel, Pulsed-Field / Q000662:veterinary; D008712:Methicillin / Q000494:pharmacology; D016106:Methicillin Resistance; D008826:Microbial Sensitivity Tests / Q000662:veterinary; D058889:Molecular Typing / Q000662:veterinary; D013203:Staphylococcal Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D013785:Thailand / Q000453:epidemiology
chemical_list: D000900:Anti-Bacterial Agents; D002981:Clindamycin; D008712:Methicillin
keywords: D-zone test; Dog; Inducible clindamycin resistance; Methicillin-resistant Staphylococcus pseudintermedius; Multidrug resistance