title: Novel Nanoscale Delivery Particles Encapsulated with Anticancer Drugs, All-trans Retinoic Acid or Curcumin, Enhance Apoptosis in Lymphoma Cells Predominantly Expressing CD20 Antigen.
abstract: BACKGROUND
  Mantle cell lymphoma (MCL), a B-cell lymphoma, pursues a relatively aggressive course, is resistant to long-term remission, and is associated with a poor prognosis. There is a pressing need for innovative treatment approaches against MCL. One such approach is targeted delivery of cytotoxic drugs to MCL cells.
  
  
  METHODS
  In the current investigation, we pursued a strategy to employ retinoid-based or curcumin-based nanoscale delivery particles, called nanodisks (NDs), for targeted drug delivery to MCL cells (Granta), and human follicular lymphoma (HF-1) cells. The cells were incubated with NDs made of CD20 single-chain variable antibody fragment (scFv)/apolipoprotein A-1 fusion protein, and loaded with either all-trans retinoic acid (ATRA) or curcumin, and cell apoptosis was measured using flow cytometry.
  
  
  CONCLUSIONS
  At 10 μM, curcumin-ND induced cell death more effectively than ATRA-ND. Combination of curcumin-ND and ATRA-ND significantly enhanced the biological activity of these drugs against lymphoma cells compared to individual treatments.
authors.affiliation: Department of Biology, Division of Natural and Social Sciences, Carthage College, Kenosha, WI, U.S.A.
authors.forename: Richard G
authors.initials: RG
authors.lastname: Stauffer
authors.affiliation: Department of Biology, Division of Natural and Social Sciences, Carthage College, Kenosha, WI, U.S.A.
authors.forename: Manar
authors.initials: M
authors.lastname: Mohammad
authors.affiliation: Department of Biology, Division of Natural and Social Sciences, Carthage College, Kenosha, WI, U.S.A. asingh1@carthage.edu.
authors.forename: Amareshwar T K
authors.initials: AT
authors.lastname: Singh
mesh_terms: D018951:Antigens, CD20 / Q000096:biosynthesis*; D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000737:chemistry; D017209:Apoptosis / Q000187:drug effects; D003474:Curcumin / Q000008:administration & dosage* / Q000737:chemistry; D004337:Drug Carriers / Q000008:administration & dosage* / Q000737:chemistry; D006801:Humans; D020522:Lymphoma, Mantle-Cell / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D014212:Tretinoin / Q000008:administration & dosage* / Q000737:chemistry
chemical_list: D018951:Antigens, CD20; D000970:Antineoplastic Agents; D004337:Drug Carriers; D014212:Tretinoin; D003474:Curcumin
keywords: Nanodisks; all-trans retinoic acid; apoptosis; curcumin; lymphoma