title: Association of inflammatory cytokine gene polymorphisms with inflammatory bowel disease in a Moroccan cohort.
abstract: The purpose of this study was to investigate whether common variants in inflammatory and immune response genes influence inflammatory bowel disease (IBD) risk among Moroccan patients. Using a candidate gene approach, 10 single-nucleotide polymorphisms mapping on six genes (MIF_rs755622, TNFA_rs1800629, IL6_rs2069840, IL6R_rs2228145, IL6ST_rs2228044, IL17A (rs2275913, rs4711998, rs7747909, rs8193036, rs3819024)) were assessed in 510 subjects grouped in 199 IBD and 311 healthy controls. Genotyping was performed with the TaqMan allelic discrimination technology. The results were analyzed using PLINK software. The frequency of allele A for TNFA rs1800629 was significantly higher in ulcerative colitis (UC) patients compared with controls (30.16 vs 16.72%; P=0.0005; odds ratio (OR)=2.15; 95% confidence interval (CI)=1.39-3.32). Statistically significant association to UC was also found under dominant AA+AG vs GG (OR=1.85, 95% CI=1.07-3.21; P=0.02) and recessive models (OR=8.38; 95% CI=2.86-24.53; P=0.0001). In the same way, an association of TNFA rs1800629 variant was observed with IBD under recessive model AA vs AG+GG (OR=4.10; 95% CI=1.56-10.76; P=0.004). No evidence of significant associations was found for the remaining investigated polymorphisms. Our data suggest that TNFA gene promoter polymorphism participates in determining IBD susceptibility in Moroccan patients.
authors.affiliation: Faculty of Medicine and Pharmacy, Laboratory of Genetics and Molecular Pathology, University Hassan II, Casablanca, Morocco.
authors.forename: N
authors.initials: N
authors.lastname: Senhaji
authors.affiliation: Instituto de Parasitología y Biomedicina López-Neyra, Consejo Superior de Investigaciones Científicas, P.T.S., Granada, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Serrano
authors.affiliation: Gastroenterology Department, CHU Ibn Rochd, Casablanca, Morocco.
authors.forename: W
authors.initials: W
authors.lastname: Badre
authors.affiliation: Faculty of Medicine and Pharmacy, Laboratory of Genetics and Molecular Pathology, University Hassan II, Casablanca, Morocco.
authors.forename: N
authors.initials: N
authors.lastname: Serbati
authors.affiliation: Pathology Department, CHU Ibn Rochd, Casablanca, Morocco.
authors.forename: M
authors.initials: M
authors.lastname: Karkouri
authors.affiliation: Laboratory of Thrombosis and Hemostasis, Montreal Heart Institute, Montreal, Quebec, Canada.
authors.forename: Y
authors.initials: Y
authors.lastname: Zaid
authors.affiliation: Faculty of Medicine and Pharmacy, Laboratory of Genetics and Molecular Pathology, University Hassan II, Casablanca, Morocco.
authors.forename: S
authors.initials: S
authors.lastname: Nadifi
authors.affiliation: Instituto de Parasitología y Biomedicina López-Neyra, Consejo Superior de Investigaciones Científicas, P.T.S., Granada, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Martin
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D003093:Colitis, Ulcerative / Q000235:genetics* / Q000276:immunology; D003424:Crohn Disease / Q000235:genetics; D016207:Cytokines / Q000235:genetics / Q000276:immunology; D005260:Female; D006801:Humans; D015212:Inflammatory Bowel Diseases / Q000235:genetics* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D009018:Morocco; D020641:Polymorphism, Single Nucleotide; D018121:Receptors, Cytokine / Q000235:genetics / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics* / Q000276:immunology; D055815:Young Adult
chemical_list: D016207:Cytokines; D018121:Receptors, Cytokine; D014409:Tumor Necrosis Factor-alpha