title: Vitamin E Supplementation Delays Cellular Senescence In Vitro.
abstract: Vitamin E is an important antioxidant that protects cells from oxidative stress-induced damage, which is an important contributor to the progression of ageing. Ageing can be studied in vitro using primary cells reaching a state of irreversible growth arrest called senescence after a limited number of cellular divisions. Generally, the most utilized biomarker of senescence is represented by the expression of the senescence associated β-galactosidase (SA-β-gal). We aimed here to study the possible effects of vitamin E supplementation in two different human primary cell types (HUVECs and fibroblasts) during the progression of cellular senescence. Utilizing an unbiased automated system, based on the detection of the SA-β-gal, we quantified cellular senescence in vitro and showed that vitamin E supplementation reduced the numbers of senescent cells during progression of ageing. Acute vitamin E supplementation did not affect cellular proliferation, whereas it was decreased after chronic treatment. Mechanistically, we show that vitamin E supplementation acts through downregulation of the expression of the cycline dependent kinase inhibitor P21. The data obtained from this study support the antiageing properties of vitamin E and identify possible mechanisms of action that warrant further investigation.
authors.affiliation: DSM Nutritional Products Ltd., R & D Human Nutrition and Health, P.O. Box 2676, 4002 Basel, Switzerland.
authors.forename: Giorgio
authors.initials: G
authors.lastname: La Fata
authors.affiliation: DSM Nutritional Products Ltd., R & D Human Nutrition and Health, P.O. Box 2676, 4002 Basel, Switzerland.
authors.forename: Nicole
authors.initials: N
authors.lastname: Seifert
authors.affiliation: DSM Nutritional Products Ltd., R & D Human Nutrition and Health, P.O. Box 2676, 4002 Basel, Switzerland.
authors.forename: Peter
authors.initials: P
authors.lastname: Weber
authors.affiliation: DSM Nutritional Products Ltd., R & D Human Nutrition and Health, P.O. Box 2676, 4002 Basel, Switzerland.
authors.forename: M Hasan
authors.initials: MH
authors.lastname: Mohajeri
mesh_terms: D000375:Aging / Q000378:metabolism; D000975:Antioxidants / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000187:drug effects*; D019587:Dietary Supplements; D005347:Fibroblasts / Q000378:metabolism; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects; D006801:Humans; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D014810:Vitamin E / Q000494:pharmacology*; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D017382:Reactive Oxygen Species; D014810:Vitamin E; D001616:beta-Galactosidase