title: Role of cytosolic and calcium independent phospholipases A(2) in insulin secretion impairment of INS-1E cells infected by S. aureus.
abstract: Cytosolic PLA2 (cPLA2) and Ca(2+)-independent PLA2 (iPLA2) play a significant role in insulin β-cells secretion. Bacterial infections may be responsible of the onset of diabetes. The mechanism by which Staphylococcus aureus infection of INS-1 cells alters glucose-induced insulin secretion has been examined. After acute infection, insulin secretion and PLA2 activities significantly increased. Moreover, increased expressions of phospho-cPLA2, phospho-PKCα and phospho-ERK 1/2 were observed. Chronic infection causes a decrease in insulin release and a significant increase of iPLA2 and COX-2 protein expression. Moreover, insulin secretion in infected cells could be restored using specific siRNAs against iPLA2 isoform and specific COX-2 inhibitor.
authors.affiliation: Dept. of Biomedical and Biotechnological Sciences, School of Medicine, University of Catania, Italy.
authors.forename: N
authors.initials: N
authors.lastname: Caporarello
authors.affiliation: Dept. of Biomedical and Biotechnological Sciences, School of Medicine, University of Catania, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Salmeri
authors.affiliation: Dept. of Biomedical and Biotechnological Sciences, School of Medicine, University of Catania, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Scalia
authors.affiliation: Dept. of Biomedical and Biotechnological Sciences, School of Medicine, University of Catania, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Motta
authors.affiliation: Dept. of Clinical and Experimental Medicine, School of Medicine, University of Catania, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Parrino
authors.affiliation: Dept. of Clinical and Experimental Medicine, School of Medicine, University of Catania, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Frittitta
authors.affiliation: Dept. of Biomedical and Biotechnological Sciences, School of Medicine, University of Catania, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Olivieri
authors.affiliation: Dept. of Biomedical and Biotechnological Sciences, School of Medicine, University of Catania, Italy.
authors.forename: M A
authors.initials: MA
authors.lastname: Toscano
authors.affiliation: Dept. of Biomedical and Biotechnological Sciences, School of Medicine, University of Catania, Italy.
authors.forename: C D
authors.initials: CD
authors.lastname: Anfuso
authors.affiliation: Dept. of Biomedical and Biotechnological Sciences, School of Medicine, University of Catania, Italy. Electronic address: lupogab@unict.it.
authors.forename: G
authors.initials: G
authors.lastname: Lupo
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D051546:Cyclooxygenase 2 / Q000737:chemistry / Q000378:metabolism; D052246:Cyclooxygenase 2 Inhibitors / Q000494:pharmacology; D003922:Diabetes Mellitus, Type 1 / Q000209:etiology; D054513:Group IV Phospholipases A2 / Q000378:metabolism*; D054522:Group VI Phospholipases A2 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D054884:Host-Pathogen Interactions / Q000187:drug effects; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D050417:Insulin-Secreting Cells / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism* / Q000382:microbiology; D007700:Kinetics; D020935:MAP Kinase Signaling System / Q000187:drug effects; D055624:Methicillin-Resistant Staphylococcus aureus / Q000502:physiology*; D010195:Pancreatitis / Q000382:microbiology / Q000503:physiopathology; D010766:Phosphorylation / Q000187:drug effects; D051571:Protein Kinase C-alpha / Q000378:metabolism; D011499:Protein Processing, Post-Translational / Q000187:drug effects; D034622:RNA Interference; D051381:Rats; D013203:Staphylococcal Infections / Q000382:microbiology / Q000503:physiopathology
chemical_list: D052246:Cyclooxygenase 2 Inhibitors; D007328:Insulin; D051546:Cyclooxygenase 2; C489691:Ptgs2 protein, rat; D051571:Protein Kinase C-alpha; D054513:Group IV Phospholipases A2; D054522:Group VI Phospholipases A2; C516391:Pla2g4a protein, rat; C535117:Pla2g6 protein, rat
keywords: INS-1E cell; Insulin; Phospholipases A(2); Staphylococcus aureus; siRNA