title: Optimization of Arundo donax Saccharification by (Hemi)cellulolytic Enzymes from Pleurotus ostreatus.
abstract: An enzymatic mixture of cellulases and xylanases was produced by Pleurotus ostreatus using microcrystalline cellulose as inducer, partially characterized and tested in the statistical analysis of Arundo donax bioconversion. The Plackett-Burman screening design was applied to identify the most significant parameters for the enzymatic hydrolysis of pretreated A. donax. As the most significant influence during the enzymatic hydrolysis of A. donax was exercised by the temperature (°C), pH, and time, the combined effect of these factors in the bioconversion by P. ostreatus cellulase and xylanase was analyzed by a 3(3) factorial experimental design. It is worth noting that the best result of 480.10 mg of sugars/gds, obtained at 45 °C, pH 3.5, and 96 hours of incubation, was significant also when compared with the results previously reached by process optimization with commercial enzymes.
authors.affiliation: Department of Chemical Sciences, University of Naples "Federico II", Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126 Naples, Italy.
authors.forename: Rossana
authors.initials: R
authors.lastname: Liguori
authors.affiliation: Institute of Agro-Environment and Forest Biology, National Research Council (CNR), Via Pietro Castellino 111, 80131 Naples, Italy.
authors.forename: Elena
authors.initials: E
authors.lastname: Ionata
authors.affiliation: Institute of Agro-Environment and Forest Biology, National Research Council (CNR), Via Pietro Castellino 111, 80131 Naples, Italy.
authors.forename: Loredana
authors.initials: L
authors.lastname: Marcolongo
authors.affiliation: Department of Bioprocess Engineering and Biotechnology, Federal University of Paraná, Coronel Francisco H. dos Santos Avenue 210, 81531-990 Curitiba, PR, Brazil.
authors.forename: Luciana Porto de Souza
authors.initials: LP
authors.lastname: Vandenberghe
authors.affiliation: Institute of Agro-Environment and Forest Biology, National Research Council (CNR), Via Pietro Castellino 111, 80131 Naples, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: La Cara
authors.affiliation: Department of Chemical Sciences, University of Naples "Federico II", Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126 Naples, Italy.
authors.forename: Vincenza
authors.initials: V
authors.lastname: Faraco
mesh_terms: D002241:Carbohydrates / Q000138:chemical synthesis* / Q000302:isolation & purification; D044602:Cellulases / Q000737:chemistry*; D002482:Cellulose / Q000737:chemistry*; D020650:Combinatorial Chemistry Techniques / Q000379:methods; D043364:Endo-1,4-beta Xylanases / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry; D020076:Pleurotus / Q000201:enzymology*; D006109:Poaceae / Q000737:chemistry*
chemical_list: D002241:Carbohydrates; D010936:Plant Extracts; D002482:Cellulose; D044602:Cellulases; D043364:Endo-1,4-beta Xylanases