title: Similar clinical features in follicular lymphomas with and without breaks in the BCL2 locus.
abstract: Approximately 15% of follicular lymphomas (FLs) lack breaks in the BCL2 locus. The aim of this study was to better define molecular and clinical features of BCL2-breakpoint/t(14;18)-negative FLs. We studied the presence of BCL2, BCL6 and MYC breaks by fluorescence in situ hybridization and the expression of BCL2, MUM1, CD10, P53 and Ki67 in large clinical trial cohorts of 540 advanced-stage FL cases and 116 early-stage disease FL patients treated with chemotherapy regimens and radiation, respectively. A total of 86% and 53% of advanced- and early-stage FLs were BCL2-breakpoint-positive, respectively. BCL2 was expressed in almost all FLs with BCL2 break and also in 86% and 69% of BCL2-breakpoint-negative advanced- and early-stage FLs, respectively. CD10 expression was significantly reduced in BCL2-breakpoint-negative FLs of all stages and MUM1 and Ki67 expression were significantly increased in BCL2-break-negative early-stage FLs. Patient characteristics did not differ between FLs with and without BCL2 breaks and neither did survival times in advanced-stage FLs. These results suggest that the molecular profile differs to some extent between FLs with and without BCL2 breaks and support the notion that FLs with and without BCL2 breaks belong to the same lymphoma entity.
authors.affiliation: Institute of Pathology, University of Würzburg, Würzburg, Germany.|Comprehensive Cancer Center Mainfranken (CCC MF), Würzburg, Germany.
authors.forename: E
authors.initials: E
authors.lastname: Leich
authors.affiliation: Institute of Medical Informatics, Biometry, and Epidemiology, University of Munich, Munich, Germany.
authors.forename: E
authors.initials: E
authors.lastname: Hoster
authors.affiliation: Institute of Pathology, University of Würzburg, Würzburg, Germany.|Comprehensive Cancer Center Mainfranken (CCC MF), Würzburg, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Wartenberg
authors.affiliation: Department of Internal Medicine III, University Hospital Munich, Munich, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Unterhalt
authors.affiliation: Institute of Human Genetics, Christian-Albrechts-University Kiel and University Hospital Schleswig-Holstein, Kiel, Germany.
authors.forename: R
authors.initials: R
authors.lastname: Siebert
authors.affiliation: Institute of Pathology, Hematopathology Section and Lymph Node Registry, University Hospital Schleswig-Holstein, Kiel, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Koch
authors.affiliation: Institute of Pathology, Hematopathology Section and Lymph Node Registry, University Hospital Schleswig-Holstein, Kiel, Germany.
authors.forename: W
authors.initials: W
authors.lastname: Klapper
authors.affiliation: Department for Radiotherapy, University Hospital Essen, Essen, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Engelhard
authors.affiliation: Institute of Pathology, University of Würzburg, Würzburg, Germany.|Comprehensive Cancer Center Mainfranken (CCC MF), Würzburg, Germany.
authors.forename: B
authors.initials: B
authors.lastname: Puppe
authors.affiliation: Margarete Fischer-Bosch Institute of Clinical Pharmacology, Robert-Bosch-Krankenhaus, Stuttgart and University of Tübingen, Tübingen, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Horn
authors.affiliation: Margarete Fischer-Bosch Institute of Clinical Pharmacology, Robert-Bosch-Krankenhaus, Stuttgart and University of Tübingen, Tübingen, Germany.
authors.forename: A M
authors.initials: AM
authors.lastname: Staiger
authors.affiliation: Institute of Human Genetics, Christian-Albrechts-University Kiel and University Hospital Schleswig-Holstein, Kiel, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Stuhlmann-Laeisz
authors.affiliation: Institute of Pathology, University Hospital Schleswig-Holstein, Lübeck, Germany.
authors.forename: H W
authors.initials: HW
authors.lastname: Bernd
authors.affiliation: Institute of Pathology, University Hospital Schleswig-Holstein, Lübeck, Germany.
authors.forename: A C
authors.initials: AC
authors.lastname: Feller
authors.affiliation: Institute of Pathology, Campus Benjamin Franklin, Charité Universitätsmedizin, Berlin, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Hummel
authors.affiliation: Institute of Pathology, Campus Benjamin Franklin, Charité Universitätsmedizin, Berlin, Germany.
authors.forename: D
authors.initials: D
authors.lastname: Lenze
authors.affiliation: Pathodiagnostik Berlin, Berlin, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Stein
authors.affiliation: Institute of Pathology, University Hospital Frankfurt am, Frankfurt, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Hartmann
authors.affiliation: Institute of Pathology, University Hospital Frankfurt am, Frankfurt, Germany.
authors.forename: M L
authors.initials: ML
authors.lastname: Hansmann
authors.affiliation: Institute of Pathology, University Hospital Ulm, Ulm, Germany.
authors.forename: P
authors.initials: P
authors.lastname: Möller
authors.affiliation: Department of Internal Medicine III, University Hospital Munich, Munich, Germany.
authors.forename: W
authors.initials: W
authors.lastname: Hiddemann
authors.affiliation: Department of Internal Medicine III, University Hospital Munich, Munich, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Dreyling
authors.affiliation: Department of Clinical Pathology, Robert-Bosch-Krankenhaus, Stuttgart, Germany.
authors.forename: G
authors.initials: G
authors.lastname: Ott
authors.affiliation: Institute of Pathology, University of Würzburg, Würzburg, Germany.|Comprehensive Cancer Center Mainfranken (CCC MF), Würzburg, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Rosenwald
mesh_terms: D019457:Chromosome Breakage; D002883:Chromosomes, Human, Pair 14 / Q000235:genetics; D002887:Chromosomes, Human, Pair 18 / Q000235:genetics; D015331:Cohort Studies; D005260:Female; D005500:Follow-Up Studies; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008224:Lymphoma, Follicular / Q000235:genetics / Q000401:mortality / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D010641:Phenotype; D011379:Prognosis; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics*; D015996:Survival Rate; D014178:Translocation, Genetic / Q000235:genetics
chemical_list: D019253:Proto-Oncogene Proteins c-bcl-2