title: Two-Phase Electrospinning to Incorporate Polyelectrolyte Complexes and Growth Factors into Electrospun Chitosan Nanofibers.
abstract: Growth factors are potent signaling proteins for tissue engineering, but they are susceptible to loss of activity when exposed to solvents used for polymer processing. This work explores preservation of fibroblast growth factor-2 (FGF-2) activity in chitosan nanofibers using two-phase electrospinning via a compound coaxial needle and from a water-in-oil emulsion FGF-2 in aqueous poly(vinyl alcohol) is added on either the inside (A/O) or the outside (O/A) of an organic chitosan phase, using the compound needle. FGF-2 is further stabilized by complexation to heparin-based nanoparticles. The emulsion method does not result in detectable incorporation of FGF-2. The A/O fibers incorporate the highest amount of FGF-2. Nanoparticle-stabilized FGF-2 in A/O nanofibers is most active toward bone-marrow stromal cells.
authors.affiliation: School of Biomedical Engineering, 1370 Campus Delivery, Fort Collins, CO, 80523-1370, USA.
authors.forename: Laura W
authors.initials: LW
authors.lastname: Place
authors.affiliation: Department of Chemical and Biological Engineering, 1370 Campus Delivery, Fort Collins, CO, 80523-1370, USA.
authors.forename: Maria
authors.initials: M
authors.lastname: Sekyi
authors.affiliation: Department of Chemical and Biological Engineering, 1370 Campus Delivery, Fort Collins, CO, 80523-1370, USA.
authors.forename: Julia
authors.initials: J
authors.lastname: Taussig
authors.affiliation: School of Biomedical Engineering, 1370 Campus Delivery, Fort Collins, CO, 80523-1370, USA.
authors.forename: Matt J
authors.initials: MJ
authors.lastname: Kipper
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism*; D048271:Chitosan / Q000737:chemistry*; D055664:Electrochemical Techniques; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000737:chemistry*; D006801:Humans; D055767:Immobilized Proteins / Q000737:chemistry; D057139:Nanofibers / Q000737:chemistry*; D011142:Polyvinyl Alcohol / Q000737:chemistry; D012756:Sheep; D017154:Stromal Cells / Q000166:cytology / Q000378:metabolism; D023822:Tissue Engineering
chemical_list: D055767:Immobilized Proteins; D016222:Fibroblast Growth Factor 2; D011142:Polyvinyl Alcohol; D048271:Chitosan