title: Deformability measurement of red blood cells using a microfluidic channel array and an air cavity in a driving syringe with high throughput and precise detection of subpopulations.
abstract: Red blood cell (RBC) deformability has been considered a potential biomarker for monitoring pathological disorders. High throughput and detection of subpopulations in RBCs are essential in the measurement of RBC deformability. In this paper, we propose a new method to measure RBC deformability by evaluating temporal variations in the average velocity of blood flow and image intensity of successively clogged RBCs in the microfluidic channel array for specific time durations. In addition, to effectively detect differences in subpopulations of RBCs, an air compliance effect is employed by adding an air cavity into a disposable syringe. The syringe was equally filled with a blood sample (V(blood) = 0.3 mL, hematocrit = 50%) and air (V(air) = 0.3 mL). Owing to the air compliance effect, blood flow in the microfluidic device behaved transiently depending on the fluidic resistance in the microfluidic device. Based on the transient behaviors of blood flows, the deformability of RBCs is quantified by evaluating three representative parameters, namely, minimum value of the average velocity of blood flow, clogging index, and delivered blood volume. The proposed method was applied to measure the deformability of blood samples consisting of homogeneous RBCs fixed with four different concentrations of glutaraldehyde solution (0%-0.23%). The proposed method was also employed to evaluate the deformability of blood samples partially mixed with normal RBCs and hardened RBCs. Thereafter, the deformability of RBCs infected by human malaria parasite Plasmodium falciparum was measured. As a result, the three parameters significantly varied, depending on the degree of deformability. In addition, the deformability measurement of blood samples was successfully completed in a short time (∼10 min). Therefore, the proposed method has significant potential in deformability measurement of blood samples containing hematological diseases with high throughput and precise detection of subpopulations in RBCs.
authors.affiliation: Department of Mechanical Engineering, Chosun University, Gwangju, Republic of Korea.
authors.forename: Yang Jun
authors.initials: YJ
authors.lastname: Kang
authors.forename: Young-Ran
authors.initials: YR
authors.lastname: Ha
authors.forename: Sang-Joon
authors.initials: SJ
authors.lastname: Lee
mesh_terms: D000388:Air; D004907:Erythrocyte Deformability; D004912:Erythrocytes / Q000166:cytology / Q000469:parasitology; D006400:Hematocrit; D006439:Hemodynamics; D006801:Humans; D056656:Lab-On-A-Chip Devices; D010963:Plasmodium falciparum / Q000502:physiology; D013594:Syringes