title: Superhydrophilic molecularly imprinted polymers based on a water-soluble functional monomer for the recognition of gastrodin in water media.
abstract: In this study, the first successfully developed superhydrophilic molecularly imprinted polymers (MIPs) for gastrodin recognition have been described. MIPs were prepared via the bulk polymerization process in an aqueous solution using alkenyl glycosides glucose (AGG) as the water-soluble functional monomer. The non-imprinted polymers (NIPs) were also synthesized using the same method without the use of the template. The dynamic water contact angles and photographs of the dispersion properties confirmed that the molecularly imprinted polymers displayed excellent superhydrophilicity. The results demonstrated that the MIPs exhibited high selectivity and an excellent imprinting effect. A molecularly imprinted solid phase extraction (MISPE) method was established. Optimization of various parameters affecting MISPE was investigated. Under the optimized conditions, a wide linear range (0.001-100.0μgmL(-1)) and low limits of detection (LOD) and quantification (LOQ) (0.03 and 0.09ngmL(-1), respectively) were achieved. When compared with the NIPs, higher recoveries (90.5% to 97.6%) of gastrodin with lower relative standard deviations values (below 6.4%) using high performance liquid chromatography were obtained at three spiked levels in three blank samples. These results demonstrated one efficient, highly selective and environmentally-friendly MISPE technique with excellent reproducibility for the purification and pre-concentration of gastrodin from an aqueous extract of Gastrodia elata roots.
authors.affiliation: Key Laboratory of TCM Quality Control Technology, Shandong Analysis and Test Center, Shandong Academy of Sciences, 19 Keyuan Street, Jinan 250014, China.
authors.forename: Wenhua
authors.initials: W
authors.lastname: Ji
authors.affiliation: School of Life Sciences, Shandong Normal University, Jinan 250014, China.
authors.forename: Mingming
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Key Laboratory of TCM Quality Control Technology, Shandong Analysis and Test Center, Shandong Academy of Sciences, 19 Keyuan Street, Jinan 250014, China.
authors.forename: Daijie
authors.initials: D
authors.lastname: Wang
authors.affiliation: Key Laboratory of TCM Quality Control Technology, Shandong Analysis and Test Center, Shandong Academy of Sciences, 19 Keyuan Street, Jinan 250014, China. Electronic address: wangx@sdas.org.
authors.forename: Xiao
authors.initials: X
authors.lastname: Wang
authors.affiliation: Key Laboratory of TCM Quality Control Technology, Shandong Analysis and Test Center, Shandong Academy of Sciences, 19 Keyuan Street, Jinan 250014, China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Liu
authors.affiliation: National Resource Center for Chinese Materia Medica, State Key Laboratory Breeding Base of Dao-di Herbs, China Academy of Chinese Medical Sciences, Beijing 100700, China. Electronic address: luqihuang@126.com.
authors.forename: Luqi
authors.initials: L
authors.lastname: Huang
mesh_terms: D001592:Benzyl Alcohols / Q000032:analysis* / Q000302:isolation & purification; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D031668:Gastrodia / Q000737:chemistry; D005960:Glucosides / Q000032:analysis* / Q000302:isolation & purification; D057230:Limit of Detection; D018517:Plant Roots / Q000737:chemistry; D058105:Polymerization; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D015203:Reproducibility of Results; D052616:Solid Phase Extraction; D014867:Water / Q000737:chemistry*
chemical_list: D001592:Benzyl Alcohols; D005960:Glucosides; D011108:Polymers; D014867:Water; C045345:gastrodin
keywords: Gastrodin; Solid phase extraction; Superhydrophilic molecularly imprinted polymers; Water media; Water-soluble functional monomer