title: Application of ultra-high performance supercritical fluid chromatography for the determination of carotenoids in dietary supplements.
abstract: A quick and simple ultra-high performance supercritical fluid chromatography-photodiode array detector method was developed and validated for the simultaneous determination of 9 carotenoids in dietary supplements. The influences of stationary phase, co-solvent, pressure, temperature and flow rate on the separation of carotenoids were evaluated. The separation of the carotenoids was carried out using an Acquity UPC(2) HSS C18 SB column (150mm×3.0mm, 1.8μm) by gradient elution with carbon dioxide and a 1:2 (v:v) methanol/ethanol mixture. The column temperature was set to 35°C and the backpressure was 15.2MPa. Under these conditions, 9 carotenoids and the internal standard, β-apo-8'-carotenal, were successfully separated within 10min. The correlation coefficients (R(2)) of the calibration curves were all above 0.997, the limits of detection for the 9 carotenoids were in the range of 0.33-1.08μg/mL, and the limits of quantification were in the range of 1.09-3.58μg/mL. The mean recoveries were from 93.4% to 109.5% at different spiking levels, and the relative standard deviations were between 0.8% and 6.0%. This method was successfully applied to the determination of 9 carotenoids in commercial dietary supplements.
authors.affiliation: Institute of Nutrition and Food Hygiene, Beijing Center for Disease Control and Prevention, Beijing 100013, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Li
authors.affiliation: Institute of Nutrition and Food Hygiene, Beijing Center for Disease Control and Prevention, Beijing 100013, China. Electronic address: gongxiaotougao@163.com.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Institute of Nutrition and Food Hygiene, Beijing Center for Disease Control and Prevention, Beijing 100013, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.affiliation: Institute of Nutrition and Food Hygiene, Beijing Center for Disease Control and Prevention, Beijing 100013, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: Institute of Nutrition and Food Hygiene, Beijing Center for Disease Control and Prevention, Beijing 100013, China.
authors.forename: Sai
authors.initials: S
authors.lastname: Fan
authors.affiliation: Institute of Nutrition and Food Hygiene, Beijing Center for Disease Control and Prevention, Beijing 100013, China.
authors.forename: Guohua
authors.initials: G
authors.lastname: Wu
authors.affiliation: Institute of Nutrition and Food Hygiene, Beijing Center for Disease Control and Prevention, Beijing 100013, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Zhao
mesh_terms: D002245:Carbon Dioxide; D002338:Carotenoids / Q000032:analysis*; D025924:Chromatography, Supercritical Fluid / Q000379:methods; D019587:Dietary Supplements / Q000032:analysis*; D000431:Ethanol; D000432:Methanol; D011312:Pressure; D012997:Solvents; D013696:Temperature
chemical_list: D012997:Solvents; C110652:beta-apo-8'-carotenal; D002245:Carbon Dioxide; D002338:Carotenoids; D000431:Ethanol; D000432:Methanol
keywords: Carotenoids; Dietary supplement; Ultra-high performance supercritical fluid chromatography