title: Polysaccharide-induced apoptosis in H22 cells through G2/M arrest and BCL2/BAX caspase-activated Fas pathway.
abstract: The aim of the present work was to investigate the effect and the mechanism of growth inhibition on mouse H22 hepatocarcinoma cell of ascitic tumor induced by cartilage polysaccharides (PS). Our results showed that PS prolonged the survival time of the mice and increased the life span. In addition, PS induced the apoptosis of the H22 cells with the typical apoptotic morphological and biochemical changes confirmed by HE staining and TUNEL assay. The subsequent analysis of cell cycle distribution and relevant proteins revealed that decrease of cells in G0/G1phase and a G2/M arrest might due to the down-regulation of Cyclin D1 and AFP and up-regulation of P21 proteins. Moreover, BCL2/BAX caspase-activated Fas pathway was activated in PS-induced H22 apoptosis.
authors.affiliation: Harbin Institute of Technology Institute of Extreme Environment Nutrition and Protection Harbin China weisong@hit.edu.cn.
authors.forename: W
authors.initials: W
authors.lastname: Song
authors.affiliation: Harbin Institute of Technology Institute of Extreme Environment Nutrition and Protection Harbin China.
authors.forename: C
authors.initials: C
authors.lastname: Song
authors.affiliation: Gaoping Shanxi China.
authors.forename: Y
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Harbin Institute of Technology Institute of Extreme Environment Nutrition and Protection Harbin China.
authors.forename: M
authors.initials: M
authors.lastname: Du
authors.affiliation: Lvliang University Department of Life Science Lvliang China.
authors.forename: P
authors.initials: P
authors.lastname: Hu
authors.affiliation: Tianjin University of Science and Technology Key Laboratory of Food Nutrition and Safety, Ministry of Education Tianjin China.
authors.forename: A
authors.initials: A
authors.lastname: Liu
authors.affiliation: Harbin Institute of Technology Institute of Extreme Environment Nutrition and Protection Harbin China lwh@hit.edu.cn.
authors.forename: W
authors.initials: W
authors.lastname: Lu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002356:Cartilage / Q000737:chemistry; D045744:Cell Line, Tumor / Q000187:drug effects; D005260:Female; D059565:G2 Phase Cell Cycle Checkpoints / Q000187:drug effects; D006801:Humans; D008113:Liver Neoplasms / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology*; D008297:Male; D051379:Mice; D011134:Polysaccharides / Q000494:pharmacology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects; D034421:Sus scrofa; D023041:Xenograft Model Antitumor Assays / Q000379:methods; D051028:bcl-2-Associated X Protein / Q000378:metabolism*; D019014:fas Receptor / Q000378:metabolism*
chemical_list: C494826:BAX protein, human; C491901:FAS protein, human; D011134:Polysaccharides; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D019014:fas Receptor