title: Dynamics of Fat Mass in DUhTP Mice Selected for Running Performance - Fat Mobilization in a Walk.
abstract: OBJECTIVE
  Reduction of body fat can be achieved by dietary programs and/or aerobic exercise training. More convenient methods to rid the body of excess fat are needed. However, it is unclear whether it is possible to more easily lose body weight at all.
  
  
  METHODS
  DUhTP mice bred through phenotype selection for high treadmill performance and unselected controls were voluntarily physically active in a running wheel over a period of 3 weeks. Phenotypical data were collected, and subcutaneous fat was analyzed for expression of mitochondria-relevant proteins.
  
  
  RESULTS
  Voluntary physical activity over 3 weeks exclusively in DUhTP mice severely reduced subcutaneous (-38%; p < 0.05) and epididymal (-32%; p < 0.05) fat. Following mild physical activity, subcutaneous fat derived from DUhTP mice showed increased levels of long chain acyl dehydrogenase (LCAD; +230%; p < 0.05) and peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC1-α; p < 0.01). Mitochondrial transcription factor A (Tfam) expression was similar in both sedentary genotypes but physical activity increased Tfam levels exclusively in DUhTP (p < 0.05).
  
  
  CONCLUSIONS
  Our findings indicate that the mitochondrial mass is highly active in DUhTP mice and responsive even to mild physical activity. While genetic predisposition could not prevent fat accretion in DUhTP mice, voluntary activity was sufficient to reduce excess body fat almost completely.
authors.affiliation: Cell Signaling Unit from the Institute for Genome Biology, Leibniz-Institute for Farm Animal Biology (FBN), Dummerstorf, Germany.
authors.forename: Julia
authors.initials: J
authors.lastname: Brenmoehl
authors.forename: Daniela
authors.initials: D
authors.lastname: Ohde
authors.forename: Christina
authors.initials: C
authors.lastname: Walz
authors.forename: Julia
authors.initials: J
authors.lastname: Schultz
authors.forename: Armin
authors.initials: A
authors.lastname: Tuchscherer
authors.forename: Florian
authors.initials: F
authors.lastname: Rieder
authors.forename: Ulla
authors.initials: U
authors.lastname: Renne
authors.forename: Andreas
authors.initials: A
authors.lastname: Hoeflich
mesh_terms: D044942:Acyl-CoA Dehydrogenase, Long-Chain / Q000378:metabolism; D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D001835:Body Weight; D004268:DNA-Binding Proteins / Q000378:metabolism; D005080:Exercise Test; D020022:Genetic Predisposition to Disease; D006609:High Mobility Group Proteins / Q000378:metabolism; D008297:Male; D051379:Mice; D008928:Mitochondria / Q000378:metabolism*; D047495:PPAR gamma / Q000378:metabolism; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; D010805:Physical Conditioning, Animal; D012420:Running / Q000502:physiology*; D050151:Subcutaneous Fat / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism; D016138:Walking
chemical_list: D004268:DNA-Binding Proteins; D006609:High Mobility Group Proteins; D047495:PPAR gamma; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; C485290:Ppargc1a protein, mouse; C081930:Tfam protein, mouse; D014157:Transcription Factors; D044942:Acyl-CoA Dehydrogenase, Long-Chain