title: Laparoscopic surgery inhibits the proliferation and metastasis of cervical cancer cells.
abstract: OBJECTIVE
  The present study is to investigate the effect of laparoscopic surgery on the proliferation and metastasis of cervical cancer cells.
  
  
  METHODS
  A total of 40 patients with phase I squamous cell carcinoma of the cervix were enrolled in the study, and divided evenly into laparoscopic surgery group and laparotomy group. In addition, another 20 patients with benign uterine lesions received laparoscopic panhysterectomy using celoscopes and were enrolled as control group. Cell apoptotic rates were determined using flow cytometry. The expression of N-myc, Fas, metastasis-associated gene 1, and nm23-H1 genes in tissues were measured using quantitative real-time polymerase chain reaction.
  
  
  RESULTS
  Cervical cancer cell apoptosis was promoted by laparoscopic surgery, but not affected by laparotomy. The expression of apoptosis suppressor gene N-myc in cervical cancer cells was reduced by laparoscopic surgery, but not affected by laparotomy. In addition, the expression of apoptosis promoting gene Fas in cervical cancer cells was enhanced by laparoscopic surgery, but not affected by laparotomy. Similarly, the expression of metastasis promoting gene MTA1 in cervical cancer cells was lowered by laparoscopic surgery, but not affected by laparotomy. Moreover, the expression of metastasis suppressor gene nm23-H1 in cervical cancer cells was increased by laparoscopic surgery, but not affected by laparotomy. Of note, laparoscopic panhysterectomy had no effect on the apoptosis or the expression of N-myc, Fas, MTA1 and nm23-H1 genes in normal cervical cells.
  
  
  CONCLUSIONS
  Laparoscopic surgery is a safe treatment method for cervical cancer. It inhibits the proliferation and metastasis of cancer cells, but has no such effects on normal cells.
authors.affiliation: Department of Gynecology and Obstetrics, Haikou Hospital Affiliated to Xiangya Medical College of Central South University Haikou 570208, P. R. China.
authors.forename: Shouguo
authors.initials: S
authors.lastname: Huang
authors.affiliation: Department of Gynecology and Obstetrics, Haikou Hospital Affiliated to Xiangya Medical College of Central South University Haikou 570208, P. R. China.
authors.forename: Jie
authors.initials: J
authors.lastname: Qin
authors.affiliation: Department of Gynecology and Obstetrics, Haikou Hospital Affiliated to Xiangya Medical College of Central South University Haikou 570208, P. R. China.
authors.forename: Jin
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Gynecology and Obstetrics, Haikou Hospital Affiliated to Xiangya Medical College of Central South University Haikou 570208, P. R. China.
authors.forename: Hong
authors.initials: H
authors.lastname: Cheng
authors.affiliation: Department of Gynecology and Obstetrics, Haikou Hospital Affiliated to Xiangya Medical College of Central South University Haikou 570208, P. R. China.
authors.forename: Qiu
authors.initials: Q
authors.lastname: Meng
authors.affiliation: Department of Gynecology and Obstetrics, Haikou Hospital Affiliated to Xiangya Medical College of Central South University Haikou 570208, P. R. China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Gynecology and Obstetrics, Haikou Hospital Affiliated to Xiangya Medical College of Central South University Haikou 570208, P. R. China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Gynecology and Obstetrics, Haikou Hospital Affiliated to Xiangya Medical College of Central South University Haikou 570208, P. R. China.
authors.forename: Huaying
authors.initials: H
authors.lastname: Li
keywords: Cervical cancer; apoptotic rate; gene expression; metastasis; proliferation