title: The LysM receptor-like kinase SlLYK10 regulates the arbuscular mycorrhizal symbiosis in tomato.
abstract: Most plants have the ability to establish a symbiosis with arbuscular mycorrhizal (AM) fungi, which allows better plant nutrition. A plant signaling pathway, called the common symbiosis signaling pathway (CSSP), is essential for the establishment of both AM and root nodule symbioses. The CSSP is activated by microbial signals. Plant receptor(s) for AM fungal signals required for the activation of the CSSP and initial fungal penetration are currently unknown. We set up conditions to use virus-induced gene silencing (VIGS) in Solanum lycopersicum to study the genes potentially involved in AM. We show that the lysin motif receptor-like kinase SlLYK10, whose orthologs in legumes are essential for nodulation, but not for AM, and SlCCaMK, a component of the CSSP, are required for penetration of the AM fungus Rhizophagus irregularis into the roots of young tomato plants. Our results support the hypothesis that the SILYK10 ancestral gene originally played a role in AM and underwent duplication and neofunctionalization for a role in nodulation in legumes. Moreover, we conclude that VIGS is an efficient method for fast screening of genes playing major roles in AM.
authors.affiliation: Laboratoire des Interactions Plantes-Microorganismes, INRA, UMR441, Castanet-Tolosan, F-31326, France.|Laboratoire des Interactions Plantes-Microorganismes, CNRS, UMR2594, Castanet-Tolosan, F-31326, France.
authors.forename: Luis
authors.initials: L
authors.lastname: Buendia
authors.affiliation: Laboratoire des Interactions Plantes-Microorganismes, INRA, UMR441, Castanet-Tolosan, F-31326, France.|Laboratoire des Interactions Plantes-Microorganismes, CNRS, UMR2594, Castanet-Tolosan, F-31326, France.
authors.forename: Tongming
authors.initials: T
authors.lastname: Wang
authors.affiliation: Laboratoire des Interactions Plantes-Microorganismes, INRA, UMR441, Castanet-Tolosan, F-31326, France.|Laboratoire des Interactions Plantes-Microorganismes, CNRS, UMR2594, Castanet-Tolosan, F-31326, France.
authors.forename: Ariane
authors.initials: A
authors.lastname: Girardin
authors.affiliation: Laboratoire des Interactions Plantes-Microorganismes, INRA, UMR441, Castanet-Tolosan, F-31326, France.|Laboratoire des Interactions Plantes-Microorganismes, CNRS, UMR2594, Castanet-Tolosan, F-31326, France.
authors.forename: Benoit
authors.initials: B
authors.lastname: Lefebvre
mesh_terms: D018506:Gene Expression Regulation, Plant; D020868:Gene Silencing; D055137:Glomeromycota / Q000502:physiology*; D018551:Solanum lycopersicum / Q000201:enzymology* / Q000235:genetics / Q000382:microbiology*; D038821:Mycorrhizae / Q000502:physiology*; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D020794:Receptor Protein-Tyrosine Kinases / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D013559:Symbiosis / Q000235:genetics
chemical_list: D010940:Plant Proteins; D020794:Receptor Protein-Tyrosine Kinases
keywords: NFP; NFR5; Solanum lycopersicum; Solyc02 g065520; arbuscular mycorrhiza/mycorrhizal (AM); calcium- and calmodulin-dependent protein kinase (CCaMK); lysin motif receptor-like kinase (LysM-RLK); tomato