title: Co-expression of Cytoskeletal Protein Adducin 3 and CD133 in Neurospheres and a Temozolomide-resistant Subclone of Glioblastoma.
abstract: BACKGROUND
  Glioma stem cells are associated for temozolomide-resistance in glioblastoma. Adducin 3 (ADD3) is a cytoskeletal protein associated with chemoresistance but its role in glioblastoma has not been investigated.
  
  
  METHODS
  Using an in vitro model of glioblastoma cells with acquired temozolomide resistance (D54-MG-R), the expressions of ADD3 and cancer stem cell markers were compared to those in temozolomide-sensitive glioblastoma cells (D54-MG-S). Immunofluorescence staining was used to investigate the expression patterns of ADD3 and cancer stem cell markers in temozolomide resistance and neurospheres of glioblastoma.
  
  
  RESULTS
  Chemoresistant cells were found to have up-regulation of ADD3 and CD133 expression. A sub-population of D54-MG-R cells and glioma neurospheres exhibited coexpression of ADD3 with CD133.
  
  
  CONCLUSIONS
  To our knowledge, this is the first report of a possible link between cytoskeletal protein expression, cancer stem cell phenotype and temozolomide resistance in human glioblastoma. This report lays the foundation for further investigation for ADD3 as a potential biomarker and therapeutic target in temozolomide-resistant glioma cells.
authors.affiliation: Department of Surgery, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Queen Mary Hospital, Hong Kong, R.O.C.
authors.forename: Ming-Wai
authors.initials: MW
authors.lastname: Poon
authors.affiliation: Department of Surgery, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Queen Mary Hospital, Hong Kong, R.O.C.
authors.forename: James Tin Fong
authors.initials: JT
authors.lastname: Zhuang
authors.affiliation: Department of Surgery, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Queen Mary Hospital, Hong Kong, R.O.C.
authors.forename: Stanley Thian Sze
authors.initials: ST
authors.lastname: Wong
authors.affiliation: Department of Surgery, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Queen Mary Hospital, Hong Kong, R.O.C.
authors.forename: Stella
authors.initials: S
authors.lastname: Sun
authors.affiliation: Department of Surgery, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Queen Mary Hospital, Hong Kong, R.O.C.
authors.forename: Xiao-Qin
authors.initials: XQ
authors.lastname: Zhang
authors.affiliation: Department of Surgery, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Queen Mary Hospital, Hong Kong, R.O.C. gilberto@hku.hk.
authors.forename: Gilberto Ka Kit
authors.initials: GK
authors.lastname: Leung
mesh_terms: D000071916:AC133 Antigen; D015703:Antigens, CD / Q000378:metabolism*; D018906:Antineoplastic Agents, Alkylating / Q000008:administration & dosage / Q000627:therapeutic use*; D017209:Apoptosis; D001932:Brain Neoplasms / Q000188:drug therapy*; D002148:Calmodulin-Binding Proteins / Q000378:metabolism*; D045744:Cell Line, Tumor; D003606:Dacarbazine / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000627:therapeutic use; D005909:Glioblastoma / Q000188:drug therapy*; D006023:Glycoproteins / Q000378:metabolism*; D006801:Humans; D014411:Neoplastic Stem Cells / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism*; D000077204:Temozolomide; D000099291:Adducins
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D018906:Antineoplastic Agents, Alkylating; D002148:Calmodulin-Binding Proteins; D003606:Dacarbazine; D006023:Glycoproteins; D010455:Peptides; D000077204:Temozolomide; D000099291:Adducins; C000606023:PROM1 protein, human
keywords: CD133; Co-expression; NANOG; adducin 3; cancer stem cell marker; cytoskeletal protein; glioblastoma; neurospheres; temozolomide-resistant subclone