title: Differential expression of 2IgB7-H3 and 4IgB7-H3 in cancer cell lines and glioma tissues.
abstract: B7-H3 protein is an important tumor antigen, but the expression of its isoforms, 4IgB7-H3 and 2IgB7-H3, in tumor tissues remains unknown due to the lack of specific monoclonal antibodies (mAbs). In the present study, a mAb (9C3) specifically recognizing 2IgB7-H3, but not 4IgB7-H3, was prepared. Using 9C3 and a previously prepared mAb (4H7) that recognizes 4IgB7-H3 and 2IgB7-H3, the differential expression of 2IgB7-H3 and 4IgB7-H3 was analyzed in a variety of tumor cell lines by flow cytometry. It was found that 4IgB7-H3 had a more broad spectrum of expression among the cell lines compared with 2IgB7-H3. The expression of the two isoforms was further examined in glioma tissues using reverse transcription-polymerase chain reaction and immunohistochemistry techniques. The data revealed that 2IgB7-H3, but not 4IgB7-H3, was specifically expressed in glioma. Taken together, these results demonstrated for the first time that 2IgB7-H3 is a valuable biomarker for the diagnosis of glioma.
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, P.R. China.
authors.forename: Zhenxin
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Cyrus Tang Hematology Center, Soochow University, Suzhou, Jiangsu 215123, P.R. China.
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, P.R. China.
authors.forename: Yanbo
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Department of Cerebral Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, P.R. China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, P.R. China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Department of Biochemistry and Molecular Biochemistry, School of Medicine, Soochow University, Suzhou, Jiangsu 215123, P.R. China.
authors.forename: Yinghui
authors.initials: Y
authors.lastname: Zhou
keywords: B7-H3; expression; glioma; monoclonal antibody