title: Identification of acetyltransferase genes (HAT1 and KAT8) regulating HBV replication by RNAi screening.
abstract: BACKGROUND
  The initiation of hepatitis B virus (HBV) replication involves the formation of covalently closed circular DNA (cccDNA) and its transcription into pregenomic RNA (pgRNA) in hepatocyte nuclei. The regulatory mechanism of HBV replication by acetyltransferase is thus far not well understood, but human acetyltransferase has been reported as being involved in the regulation of HBV replication.
  
  
  RESULTS
  Depletion of KAT8 or HAT1 via RNA interference (RNAi) markedly down-regulated HBV-DNA and pgRNA levels in HepG2.2.15 cells, with KAT8 knockdown reducing both HBsAg and HBeAg more than HAT1 knockdown. Consistent with these observations, HBV replication regulators hepatocyte nuclear factor-4-α (HNF4α) and peroxisome proliferator-activated receptor gamma coactivator- (PPARGC-) 1-α were decreased following knockdown of HAT1 or KAT8.
  
  
  CONCLUSIONS
  These data suggest that KAT8 or HAT1 regulate HBV replication and may be potential drug targets of anti-HBV therapy.
authors.affiliation: Department of Infectious Diseases, Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Infectious Diseases, Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
authors.forename: KeHui
authors.initials: K
authors.lastname: Liu
authors.affiliation: Discipline of Pathology, School of Medical Sciences and The Bosch Institute, Charles Perkins Centre, The University of Sydney, Sydney, NSW 2006 Australia.|Central Clinical School, Sydney Medical School, The University of Sydney, Sydney, NSW 2006 Australia.
authors.forename: Bernard A M
authors.initials: BAM
authors.lastname: Fang
authors.affiliation: Department of Infectious Diseases, Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.|Department of Cardiology, Shanghai First People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
authors.forename: HaiQing
authors.initials: H
authors.lastname: Wu
authors.affiliation: Department of Infectious Diseases, Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
authors.forename: FengDi
authors.initials: F
authors.lastname: Li
authors.affiliation: Department of Infectious Diseases, Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
authors.forename: XiaoGang
authors.initials: X
authors.lastname: Xiang
authors.affiliation: Department of Infectious Diseases, Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
authors.forename: WeiLiang
authors.initials: W
authors.lastname: Tang
authors.affiliation: Department of Infectious Diseases, Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
authors.forename: GangDe
authors.initials: G
authors.lastname: Zhao
authors.affiliation: Department of Infectious Diseases, Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
authors.forename: LanYi
authors.initials: L
authors.lastname: Lin
authors.affiliation: Department of Infectious Diseases, Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.|Discipline of Pathology, School of Medical Sciences and The Bosch Institute, Charles Perkins Centre, The University of Sydney, Sydney, NSW 2006 Australia.
authors.forename: Shisan
authors.initials: S
authors.lastname: Bao
authors.affiliation: Department of Infectious Diseases, Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Xie
keywords: KAT8, HAT1, HBV replication; RNAi screening; pgRNA