title: Development and validation of a simple high performance thin layer chromatography method combined with direct 1,1-diphenyl-2-picrylhydrazyl assay to quantify free radical scavenging activity in wine.
abstract: The aim of this study was to: (a) develop a simple, high performance thin layer chromatographic (HPTLC) method combined with direct 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay to rapidly assess and compare free radical scavenging activity or anti-oxidant activity for major classes of polyphenolics present in wines; and (b) to investigate relationship between free radical scavenging activity to the total polyphenolic content (TPC) and total antioxidant capacity (TAC) in the wine samples. The most potent free radical scavengers that we tested for in the wine samples were found to be resveratrol (polyphenolic non-flavonoid) and rutin (flavonoid), while polyphenolic acids (caffeic acid and gallic acid) although present in all wine samples were found to be less potent free radical scavengers. Therefore, the total antioxidant capacity was mostly affected by the presence of resveratrol and rutin, while total polyphenolic content was mostly influenced by the presence of the less potent free radical scavengers gallic and caffeic acids.
authors.affiliation: Faculty of Pharmacy, Universiti Teknologi MARA, Puncak Alam Campus, Bandar Puncak Alam, Selangor 42300, Malaysia.
authors.forename: Snezana
authors.initials: S
authors.lastname: Agatonovic-Kustrin
authors.affiliation: School of Pharmacy and Applied Science, La Trobe Institute of Molecular Sciences, La Trobe University, Edwards Rd, Bendigo 3550, Australia. Electronic address: d.morton@latrobe.edu.au.
authors.forename: David W
authors.initials: DW
authors.lastname: Morton
authors.affiliation: Faculty of Medicine, Universiti Teknologi MARA, Sg. Buloh Campus, 47000, Sungai Buloh, Selangor, Malaysia.
authors.forename: Ahmad P
authors.initials: AP
authors.lastname: Yusof
mesh_terms: D000975:Antioxidants / Q000032:analysis; D001713:Biphenyl Compounds / Q000032:analysis*; D002109:Caffeic Acids / Q000032:analysis; D002855:Chromatography, Thin Layer / Q000379:methods*; D016166:Free Radical Scavengers / Q000032:analysis*; D005707:Gallic Acid / Q000032:analysis; D010851:Picrates / Q000032:analysis*; D059808:Polyphenols / Q000032:analysis; D015203:Reproducibility of Results; D000077185:Resveratrol; D012431:Rutin / Q000032:analysis; D013267:Stilbenes / Q000032:analysis; D014920:Wine / Q000032:analysis*
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D002109:Caffeic Acids; D016166:Free Radical Scavengers; D010851:Picrates; D059808:Polyphenols; D013267:Stilbenes; D012431:Rutin; D005707:Gallic Acid; C004931:1,1-diphenyl-2-picrylhydrazyl; D000077185:Resveratrol; C040048:caffeic acid
keywords: 1,1-Diphenyl-2-picrylhydrazyl; Free radical scavenging activity; High performance thin layer chromatography; High resolution plate imaging; Polyphenolic compounds; Wine antioxidants