title: Matrix metalloproteinase-3 gene polymorphism and its mRNA expression in rheumatoid arthritis.
abstract: Matrix metalloproteinase-3 (MMP-3) can mediate the occurrence and development of rheumatoid arthritis (RA). The MMP3 promoter gene exhibits polymorphism with 5A/6A alleles. We investigated the correlation between the expression of MMP3 gene polymorphism and RA to provide an objective basis for prognosis evaluation. We enrolled 80 RA patients and 80 healthy subjects. Enzyme-linked immunosorbent assay was used to detect MMP-3 serum levels, pyrosequencing was used to test MMP3 genotypes, and real-time polymerase chain reaction determined MMP-3 mRNA expression levels. Compared with the control group, the serum level of MMP-3 in the RA patients increased significantly (P < 0.05). The serum level of MMP-3 in RA patients in the active period was markedly elevated compared with that in patients in the relief period (P < 0.05). There was no statistically significant difference between MMP3 gene frequency distribution in the RA patients and the control group (P > 0.05). MMP-3 mRNA expression in the RA patients was markedly upregulated compared with the control group (P < 0.05), while RA patients in the active period exhibited higher MMP-3 mRNA expression (P < 0.05). There was no significant difference in MMP-3 mRNA expression between RA patients with or without the 6A/6A genotype (P > 0.05). RA patients exhibited higher serum MMP-3 levels and mRNA expression, which were more obvious in the active period. MMP-3 is associated with the occurrence and development of RA bone erosion, and its serum level and mRNA expression can be treated as important predictors of joint damage.
authors.affiliation: Department of Surgery, Victory Hospital of China Petrochemical Group, Victory Petroleum Administration, Dongying, Shandong, China.
authors.forename: M J
authors.initials: MJ
authors.lastname: Ma
authors.affiliation: Department of Orthopedics, Affiliated Hospital of Weifang Medical University, Weifang, Shandong, China.
authors.forename: H C
authors.initials: HC
authors.lastname: Liu
authors.affiliation: Orthopaedic Institute of PLA, 89th Hospital, Weifang, Shandong, China.
authors.forename: X Q
authors.initials: XQ
authors.lastname: Qu
authors.affiliation: Orthopaedic Institute of PLA, 89th Hospital, Weifang, Shandong, China.
authors.forename: J L
authors.initials: JL
authors.lastname: Wang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000483:Alleles; D001172:Arthritis, Rheumatoid / Q000097:blood / Q000175:diagnosis / Q000235:genetics*; D016022:Case-Control Studies; D005260:Female; D015870:Gene Expression; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D008297:Male; D019278:Matrix Metalloproteinase 3 / Q000097:blood / Q000235:genetics*; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics*; D055815:Young Adult
chemical_list: D012333:RNA, Messenger; D019278:Matrix Metalloproteinase 3