title: Anti-Outer membrane protein C antibodies in colorectal neoplasia.
abstract: Sporadic colorectal cancer (CRC) represents an enormous problem worldwide. Large intestinal microbiota play an important role in the colorectal carcinogenesis. The aim of the study was to investigate anti-Outer membrane protein C (anti-OmpC) antibodies, aimed at porin C, which is embedded in the outer membrane of gram-negative bacteria, in patients with colorectal adenoma (CRA), CRC and controls. The study included 22 patients with CRA (11 men, 11 women, aged 26-79, mean 65 ± 12), 11 patients with CRC (9 men, 2 women, aged 50-83, mean 66 ± 11) and 45 controls, blood donors (24 men, 21 women, aged 20-58, mean 38 ± 10). Serum anti-OmpC antibodies were investigated by means of ELISA. Values of 0-20 U/mL were considered to be negative; values >25 U/mL were assessed as positive. A total of 9/11 (82 %) patients with CRC had positive anti-OmpC antibodies. Anti-OmpC antibodies were negative or grey-zone in 37/45 (82 %) controls. Serum anti-OmpC were found to be significantly higher in patients with CRC (median 42.4, interquartile range (IQR) 22.2) compared to controls (median 18.3, IQR 12.4), p < 0.001. No statistically significant difference in anti-OmpC was found between controls (median 18.3, IQR 12.4) and CRA patients (median 17.7, IQR 16.5), p = 0.326. Anti-OmpC were significantly higher in patients with CRC (median 42.4, IQR 22.2) compared to patients with CRA (median 17.7, IQR 16.5), p = 0.011. Positivity of anti-OmpC antibodies was found in patients with CRC, which supports the contribution of gram-negative large intestinal microbiota to the pathogenesis of CRC.
authors.affiliation: 2nd Department of Internal Medicine - Gastroenterology, Charles University in Prague, Faculty of Medicine at Hradec Kralove, University Teaching Hospital, Sokolska 581, 50005, Hradec Kralove, Czech Republic. darina.kohoutova@seznam.cz.|Division of Surgery and Interventional Science, University College London, 67-73 Riding House Street, London, W1W 7EJ, UK. darina.kohoutova@seznam.cz.
authors.forename: D
authors.initials: D
authors.lastname: Kohoutova
authors.affiliation: Department of Clinical Immunology and Allergology, Charles University in Prague, Faculty of Medicine at Hradec Kralove, University Teaching Hospital, Sokolska 581, 50005, Hradec Kralove, Czech Republic.
authors.forename: M
authors.initials: M
authors.lastname: Drahosova
authors.affiliation: Department of Mathematics, Faculty of Science, University of Hradec Kralove, Jana Koziny 1237, 500 03, Hradec Kralove, Czech Republic.
authors.forename: M
authors.initials: M
authors.lastname: Cihak
authors.affiliation: 2nd Department of Internal Medicine - Gastroenterology, Charles University in Prague, Faculty of Medicine at Hradec Kralove, University Teaching Hospital, Sokolska 581, 50005, Hradec Kralove, Czech Republic.
authors.forename: P
authors.initials: P
authors.lastname: Moravkova
authors.affiliation: 2nd Department of Internal Medicine - Gastroenterology, Charles University in Prague, Faculty of Medicine at Hradec Kralove, University Teaching Hospital, Sokolska 581, 50005, Hradec Kralove, Czech Republic.
authors.forename: J
authors.initials: J
authors.lastname: Bures
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000907:Antibodies, Bacterial / Q000097:blood*; D015179:Colorectal Neoplasms / Q000473:pathology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D018272:Porins / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; C113724:OmpC protein; D018272:Porins