title: FAM172A is a tumor suppressor in colorectal carcinoma.
abstract: The present study was designed to elucidate the regulatory role of a novel protein FAM172A in carcinogenesis of colorectal carcinoma (CRC). Investigation of clinical samples using Western blotting showed that expression of FAM172A is significantly lower in cancerous tissues than in adjacent tissues. Furthermore, we constructed in vitro model for continuous overexpression and silencing of FAM172A with a retroviral vector system. FAM172A suppressed the proliferative and invasive potentials of LOVO cells as shown in MTT test, transwell migration assay, wound healing assay, 3D-culture morphologic study, and xenograft experiment. RT-PCR and Western blotting showed that FAM172A overexpression inhibited expressions of Cyclin D1, CDK2, MMP-2, MMP-9, PERK, elF2α, ATF6, XBP1, and GRP78, while FAM172A silencing induced their expressions. FAM172A might regulate ERS through PERK-elF2α, ATF6-XBP1-GRP78 signal pathway. The results implicated that FAM172A functioned as a tumor suppressor in colorectal carcinoma.
authors.affiliation: Department of General Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou, 510282, People's Republic of China.
authors.forename: Chunhui
authors.initials: C
authors.lastname: Cui
authors.affiliation: Department of General Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou, 510282, People's Republic of China.
authors.forename: Lili
authors.initials: L
authors.lastname: Ye
authors.affiliation: Department of General Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou, 510282, People's Republic of China.
authors.forename: Zonghai
authors.initials: Z
authors.lastname: Huang
authors.affiliation: Department of Ophthalmology, Zhujiang Hospital, Southern Medical University, Guangzhou, 510282, People's Republic of China.
authors.forename: Shuxin
authors.initials: S
authors.lastname: Huang
authors.affiliation: Department of Vascular Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, People's Republic of China. haoflliu@sina.com.
authors.forename: Hao
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of General Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou, 510282, People's Republic of China. jinlonggyu@163.com.
authors.forename: Jinlong
authors.initials: J
authors.lastname: Yu
mesh_terms: D000818:Animals; D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D004195:Disease Models, Animal; D000091342:Endoplasmic Reticulum Chaperone BiP; D015972:Gene Expression Regulation, Neoplastic; D020868:Gene Silencing; D016147:Genes, Tumor Suppressor; D064593:Heterografts; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism; D011506:Proteins / Q000235:genetics* / Q000378:metabolism; D047368:Tumor Burden
chemical_list: D000091342:Endoplasmic Reticulum Chaperone BiP; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; D011506:Proteins; C550020:ARB2A protein, human; C000717247:HSPA5 protein, human
keywords: Colorectal carcinoma; FAM172A; Tumor suppressor