title: Somatic Embryogenesis in Lisianthus (Eustoma russellianum Griseb.).
abstract: Somatic embryogenesis is, for the main floricultural crops, a promising system for commercial scale-up, providing cloned material to be traded as seedlings. Somatic embryos, having the contemporary presence of root apical meristem and shoot apical meristem, can be readily acclimatized. For Lisianthus it is possible to induce embryogenic callus from leaf fragments of selected genotypes and to obtain embryos either in agarized substrate or in liquid suspension culture. The production of somatic embryos in liquid medium is high and can be modulated in order to synchronize the cycle and the size of the neoformed structures. The possibility to use the liquid substrate with high propagation rates reduces labor costs and could support the costs of eventual automation. In this paper we report a stepwise protocol for somatic embryogenesis in the species Eustoma russellianum.
authors.affiliation: Ornamental Plants Research Unit, Consiglio per la ricerca in agricoltura e l' analisi dell' economia agraria (CREA), Corso Inglesi 508, 18038, Sanremo (Imperia), Italy. barbara.ruffoni@entecra.it.
authors.forename: Barbara
authors.initials: B
authors.lastname: Ruffoni
authors.affiliation: Ornamental Plants Research Unit, Consiglio per la ricerca in agricoltura e l' analisi dell' economia agraria (CREA), Corso Inglesi 508, 18038, Sanremo (Imperia), Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Bassolino
mesh_terms: D029965:Gentianaceae / Q000235:genetics / Q000254:growth & development*; D018519:Meristem / Q000235:genetics / Q000254:growth & development; D063245:Plant Development / Q000235:genetics; D018515:Plant Leaves / Q000235:genetics / Q000254:growth & development; D018517:Plant Roots / Q000235:genetics / Q000254:growth & development*; D058439:Plant Somatic Embryogenesis Techniques / Q000379:methods*; D030821:Plants, Genetically Modified / Q000235:genetics; D036226:Seedlings / Q000235:genetics / Q000254:growth & development; D046509:Tissue Culture Techniques / Q000379:methods*
keywords: 2,4-D; Cell suspension; Conversion; Lisianthus; Somatic embryo s; Somatic embryo--> maturation