title: Molecular Basis of Laminin-Integrin Interactions.
abstract: Laminins are composed of three polypeptide chains, designated as α, β, and γ. The C-terminal region of laminin heterotrimers, containing coiled-coil regions, short tails, and laminin globular (LG) domains, is necessary and sufficient for binding to integrins, which are the major laminin receptor class. Laminin recognition by integrins critically requires the α chain LG domains and a glutamic acid residue of the γ chain at the third position from the C-terminus. Furthermore, the C-terminal region of the β chain contains a short amino acid sequence that modulates laminin affinity for integrins. Thus, all three of the laminin chains act cooperatively to facilitate integrin binding. Mammals possess 5 α (α1-5), 3 β (β1-3), and 3 γ (γ1-3) chains, combinations of which give rise to 16 distinct laminin isoforms. Each isoform is expressed in a tissue-specific and developmental stage-specific manner, exerting its functions through binding of integrins. In this review, we detail the current knowledge surrounding the molecular basis and physiological relevance of specific interactions between laminins and integrins, and describe the mechanisms underlying laminin action through integrins.
authors.affiliation: Laboratory of Extracellular Matrix Biochemistry, Institute for Protein Research, Osaka University, Suita, Osaka, Japan.
authors.forename: Masashi
authors.initials: M
authors.lastname: Yamada
authors.affiliation: Laboratory of Extracellular Matrix Biochemistry, Institute for Protein Research, Osaka University, Suita, Osaka, Japan.
authors.forename: Kiyotoshi
authors.initials: K
authors.lastname: Sekiguchi
mesh_terms: D000818:Animals; D006801:Humans; D016023:Integrins / Q000378:metabolism*; D007797:Laminin / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D017474:Receptors, Laminin / Q000378:metabolism; D013379:Substrate Specificity; D059470:Tetraspanins / Q000378:metabolism
chemical_list: D016023:Integrins; D007797:Laminin; D017474:Receptors, Laminin; D059470:Tetraspanins
keywords: Basement membrane; CD151; Extracellular matrix; Integrin; Laminin; Pluripotent stem cells; Tetraspanin