title: Dihydromyricetin induces cell apoptosis via a p53-related pathway in AGS human gastric cancer cells.
abstract: The aim of the present study was to determine the anti-proliferative and pro-apoptotic effects of dihydromyricetin (DHM) on the AGS human gastric cancer cells and their underlying mechanisms. The effects of DHM on AGS cells were evaluated by using 3-(4, 5-di-methylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), lactate dehydrogenase, and Annexin V/propidium iodide (PI) double-staining assays. The underlying mechanisms were determined by using quantitative real-time polymerase chain reaction. The results demonstrated that DHM significantly (P < 0.05) inhibited AGS cell proliferation and induced cell cytotoxicity in a dose- and time-dependent manner. Additionally, Annexin V/PI double-staining assay showed that DHM promoted cell apoptosis in both, early and late stages. Furthermore, DHM also regulated the expression of apoptotic genes such as p53 and B-cell lymphoma-2 (bcl-2) in a dose- and time-dependent manner. In conclusion, this is the first report demonstrating the anticancer and pro-apoptosis effects of DHM on AGS human gastric cancer cells. The results strongly suggest that DHM may be a potential therapeutic candidate for the treatment of gastric cancer.
authors.affiliation: Department of General Surgery, The China Japan Friendship Hospital of Jilin University, Changchun, China.
authors.forename: F J
authors.initials: FJ
authors.lastname: Ji
authors.affiliation: Department of General Surgery, The China Japan Friendship Hospital of Jilin University, Changchun, China.
authors.forename: X F
authors.initials: XF
authors.lastname: Tian
authors.affiliation: Department of General Surgery, Jilin Central Hospital, Jilin, China.
authors.forename: X W
authors.initials: XW
authors.lastname: Liu
authors.affiliation: Department of General Surgery, People's Hospital of Panan County, Zhejiang, China.
authors.forename: L B
authors.initials: LB
authors.lastname: Fu
authors.affiliation: Department of General Surgery, The China Japan Friendship Hospital of Jilin University, Changchun, China.
authors.forename: Y Y
authors.initials: YY
authors.lastname: Wu
authors.affiliation: Department of General Surgery, The China Japan Friendship Hospital of Jilin University, Changchun, China.
authors.forename: X D
authors.initials: XD
authors.lastname: Fang
authors.affiliation: Department of General Surgery, The China Japan Friendship Hospital of Jilin University, Changchun, China jinhongyong119@sina.com.
authors.forename: H Y
authors.initials: HY
authors.lastname: Jin
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects* / Q000235:genetics; D051017:Apoptosis Regulatory Proteins / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D044948:Flavonols / Q000494:pharmacology*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D015398:Signal Transduction / Q000187:drug effects*; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D051017:Apoptosis Regulatory Proteins; D044948:Flavonols; D016159:Tumor Suppressor Protein p53; C472036:dihydromyricetin