title: Mechanism of protective immunity by vaccination with recombinantEchinococcus granulosusglutathione S-transferase (Chinese strain) in mice.
abstract: The aim of this study was to investigate the immunoprotective effects of recombinantEchinococcus granulosusglutathione S-transferase (rEgGST) against the development of protoscolices (PSCs), and to determine the mechanisms underlying this protection. ICR mice were subcutaneously immunized three times with rEgGST at weeks 0, 2 and 4, followed by the intraperitoneal administration ofE. granulosusPSCs at week 10. Six mice in each group were sacrificed at 0, 2, 4, 6, 10, 18 and 30 weeks following the initial vaccination in order to observe the macroscopic and microscopic effects of parasite development. Various analyses were subsequently conducted, including determination of the levels of immunoglobulins (Igs) and cytokines. Significant differences were observed a number of indices of immune response following immunization with rEgGST. These included reduced cyst formation and elevated levels of IgG1, IgG2a, IgG3, IL-2, IL-4, IL-10 and IFN-γ, which indicated an increased percentage of immune helper cells. The results of the present study suggest that immunization with rEgGST in mice is able to successfully reduce the PSC-induced formation of cysts and to stimulate an immune response, suggesting that rEgGST possesses potential value as a candidate vaccine for PSC infection.
authors.affiliation: Center of Scientific Technology, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China.
authors.forename: Mingxing
authors.initials: M
authors.lastname: Zhu
authors.affiliation: Institute of Clinical Laboratory, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China.
authors.forename: Xiuqing
authors.initials: X
authors.lastname: Wang
authors.affiliation: School of Basic Medical Sciences, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China.
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
authors.affiliation: Experimental Animal Center, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China.
authors.forename: Zhisheng
authors.initials: Z
authors.lastname: Wang
authors.affiliation: School of Basic Medical Sciences, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China.
authors.forename: Jiaqing
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Center of Scientific Technology, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China.
authors.forename: Yana
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Center of Scientific Technology, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhao
keywords: Echinococcus granulosus; immunoprotection; recombinant Echinococcus granulosus glutathione S-transferase; vaccine