title: Biological conversion of biogas to methanol using methanotrophs isolated from solid-state anaerobic digestate.
abstract: The aim of this work was to isolate methanotrophs (methane oxidizing bacteria) that can directly convert biogas produced at a commercial anaerobic digestion (AD) facility to methanol. A methanotrophic bacterium was isolated from solid-state anaerobic digestate. The isolate had characteristics comparable to obligate methanotrophs from the genus Methylocaldum. This newly isolated methanotroph grew on biogas or purified CH4 and successfully converted biogas from AD to methanol. Methanol production was achieved using several methanol dehydrogenase (MDH) inhibitors and formate as an electron donor. The isolate also produced methanol using phosphate with no electron donor or using formate with no MDH inhibitor. The maximum methanol concentration (0.43±0.00gL(-1)) and 48-h CH4 to methanol conversion (25.5±1.1%) were achieved using biogas as substrate and a growth medium containing 50mM phosphate and 80mM formate.
authors.affiliation: Department of Food, Agricultural and Biological Engineering, The Ohio State University/Ohio Agricultural Research and Development Center, 1680 Madison Ave., Wooster, OH 44691-4096, USA.
authors.forename: Johnathon P
authors.initials: JP
authors.lastname: Sheets
authors.affiliation: Department of Food, Agricultural and Biological Engineering, The Ohio State University/Ohio Agricultural Research and Development Center, 1680 Madison Ave., Wooster, OH 44691-4096, USA.
authors.forename: Xumeng
authors.initials: X
authors.lastname: Ge
authors.affiliation: Department of Animal Sciences, The Ohio State University, Columbus, OH 43210, USA.
authors.forename: Yueh-Fen
authors.initials: YF
authors.lastname: Li
authors.affiliation: Department of Animal Sciences, The Ohio State University, Columbus, OH 43210, USA.
authors.forename: Zhongtang
authors.initials: Z
authors.lastname: Yu
authors.affiliation: Department of Food, Agricultural and Biological Engineering, The Ohio State University/Ohio Agricultural Research and Development Center, 1680 Madison Ave., Wooster, OH 44691-4096, USA. Electronic address: li.851@osu.edu.
authors.forename: Yebo
authors.initials: Y
authors.lastname: Li
mesh_terms: D000429:Alcohol Oxidoreductases / Q000037:antagonists & inhibitors; D000693:Anaerobiosis; D056804:Biofuels; D003470:Culture Media; D000432:Methanol / Q000378:metabolism*; D008772:Methylococcaceae / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D056804:Biofuels; D003470:Culture Media; D000429:Alcohol Oxidoreductases; C019641:alcohol dehydrogenase (acceptor); D000432:Methanol
keywords: Anaerobic digestion; Biogas; Biological conversion; Methanol; Methanotrophs