title: The killing of macrophages by Corynebacterium ulcerans.
abstract: Corynebacterium ulcerans is an emerging pathogen transmitted by a zoonotic pathway with a very broad host spectrum to humans. Despite rising numbers of infections and potentially fatal outcomes, data on the molecular basis of pathogenicity are scarce. In this study, the interaction of 2 C. ulcerans isolates - one from an asymptomatic dog, one from a fatal case of human infection - with human macrophages was investigated. C. ulcerans strains were able to survive in macrophages for at least 20 hours. Uptake led to delay of phagolysosome maturation and detrimental effects on the macrophages as deduced from cytotoxicity measurements and FACS analyses. The data presented here indicate a high infectious potential of this emerging pathogen.
authors.affiliation: a Friedrich-Alexander-Universität Erlangen-Nürnberg; Professur für Mikrobiologie ; Erlangen , Germany.
authors.forename: Elena
authors.initials: E
authors.lastname: Hacker
authors.affiliation: a Friedrich-Alexander-Universität Erlangen-Nürnberg; Professur für Mikrobiologie ; Erlangen , Germany.
authors.forename: Lisa
authors.initials: L
authors.lastname: Ott
authors.affiliation: b Friedrich-Alexander-Universität Erlangen-Nürnberg; Universitätsklinikum Erlangen; Mikrobiologisches Institut - Klinische Mikrobiologie; Immunologie und Hygiene ; Erlangen , Germany.
authors.forename: Jan
authors.initials: J
authors.lastname: Schulze-Luehrmann
authors.affiliation: b Friedrich-Alexander-Universität Erlangen-Nürnberg; Universitätsklinikum Erlangen; Mikrobiologisches Institut - Klinische Mikrobiologie; Immunologie und Hygiene ; Erlangen , Germany.
authors.forename: Anja
authors.initials: A
authors.lastname: Lührmann
authors.affiliation: c Fraunhofer Institut für Integrierte Schaltungen (IIS) ; Erlangen , Germany.
authors.forename: Veit
authors.initials: V
authors.lastname: Wiesmann
authors.affiliation: c Fraunhofer Institut für Integrierte Schaltungen (IIS) ; Erlangen , Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Wittenberg
authors.affiliation: a Friedrich-Alexander-Universität Erlangen-Nürnberg; Professur für Mikrobiologie ; Erlangen , Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Burkovski
mesh_terms: D000369:Aged, 80 and over; D000818:Animals; D002460:Cell Line; D003352:Corynebacterium / Q000276:immunology / Q000302:isolation & purification / Q000472:pathogenicity*; D003354:Corynebacterium Infections / Q000382:microbiology* / Q000662:veterinary*; D016207:Cytokines / Q000276:immunology; D004283:Dog Diseases / Q000382:microbiology* / Q000473:pathology; D004285:Dogs; D005260:Female; D006801:Humans; D008264:Macrophages / Q000166:cytology / Q000378:metabolism / Q000382:microbiology* / Q000473:pathology*; D010588:Phagosomes / Q000382:microbiology
chemical_list: D016207:Cytokines
keywords: Corynebacterium ulcerans; FACS; THP-1 cells; host-pathogen interaction; necrotic cell death