title: Search for the genome of bovine herpesvirus types 1, 4 and 5 in bovine semen.
abstract: Bovine herpesvirus type 1 (BoHV-1) causes respiratory and reproductive disorders in cattle. Recently, bovine herpesvirus type 5 (BoHV-5) and bovine herpesvirus type 4 (BoHV-4) have been identified to be associated with genital disease. In this study, the presence of the genome of BoHV-1, BoHV-4 and BoHV-5 in bovine semen of Argentinean and international origin was analyzed by PCR assays. The most important finding of this study is the detection of the genome of BoHV-1 and BoHV-4 in semen of bulls maintained at artificial insemination centers. It is particularly relevant that BoHV-1 DNA was also identified in one sample of international origin suggesting the need for extensive quality control measures on international transport of bovine semen.
authors.affiliation: Facultad de Ciencias Veterinarias, UNCPBA, Tandil (7000), Argentina.
authors.forename: P E
authors.initials: PE
authors.lastname: Morán
authors.affiliation: Becaria FONCyT - Agencia Nacional de Promoción Científica y Tecnológica, Argentina.
authors.forename: P A
authors.initials: PA
authors.lastname: Favier
authors.affiliation: Instituto de Virología - INTA Castelar, Castelar (B1712WAA), Argentina.
authors.forename: M
authors.initials: M
authors.lastname: Lomónaco
authors.affiliation: Facultad de Ciencias Veterinarias, UNCPBA, Tandil (7000), Argentina.
authors.forename: M C
authors.initials: MC
authors.lastname: Catena
authors.affiliation: Facultad de Ciencias Veterinarias, UNCPBA, Tandil (7000), Argentina ; Consejo Nacional de Investigaciones Científicas y Técnicas CONICET - CIVETAN (Centro de Investigación Veterinaria de Tandil), Argentina.
authors.forename: M L
authors.initials: ML
authors.lastname: Chiapparrone
authors.affiliation: Estación Experimental Agropecuaria - INTA Balcarce, Balcarce (7620), Argentina.
authors.forename: A C
authors.initials: AC
authors.lastname: Odeón
authors.affiliation: Estación Experimental Agropecuaria - INTA Balcarce, Balcarce (7620), Argentina.
authors.forename: A E
authors.initials: AE
authors.lastname: Verna
authors.affiliation: Facultad de Ciencias Veterinarias, UNCPBA, Tandil (7000), Argentina ; Consejo Nacional de Investigaciones Científicas y Técnicas CONICET - CIVETAN (Centro de Investigación Veterinaria de Tandil), Argentina.
authors.forename: S E
authors.initials: SE
authors.lastname: Pérez
keywords: Artificial insemination; Bovine herpesviruses; Genome; Semen