title: Genetic association between CD44 polymorphisms and chronic hepatitis B virus infection in a Chinese Han population.
abstract: OBJECTIVE
  This article aimed at discussing the association of chronic hepatitis B virus (HBV) infection with CD44 polymorphisms in Chinese Han population; meanwhile, the interaction of polymorphisms was also analyzed based on chronic HBV infection.
  
  
  METHODS
  The genotyping of CD44 polymorphisms was conducted by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in 108 HBV infected and 130 healthy persons. The genotype distributions of CD44 rs187115, rs13347 in the control group were checked by Hardy-Weinberg equilibrium (HWE). The strength of the relevance between polymorphism and disease was measured by odds ratio (OR) with corresponding 95% confidence interval (CI) calculated by χ(2) test. The 2×4 crossover analysis method was used to conduct the interaction analysis of polymorphisms.
  
  
  RESULTS
  The genotype distributions in controls conformed to HWE. GG genotype and G allele frequencies in rs187115 were obviously higher in cases than the controls (P=0.02, 0.04). Compared with the common genotype CC, individual who carried mutant genotypes (CT and TT) of rs13347 had a significantly high risk to suffer from HBV infection (OR=1.99, P=0.02 for CT; OR=3.56, P=3.00×10(-3) for TT), furthermore, CT+TT genotype also showed a high susceptibility (OR=2.27, P=2.00×10(-3)). Similarly, T allele of rs13347 increased 0.98 times risk in cases compared with controls (OR=1.98, 95% CI=1.34-2.92). The two polymorphisms in CD44 presented a positive interaction.
  
  
  CONCLUSIONS
  CD44 polymorphisms are associated with chronic HBV infection as the risk factors, and the synergistic action is also found between the two polymorphisms.
authors.affiliation: Liver Fibrosis Diagnosis and Treatment Center, 302 Military Hospital of China Beijing 100039, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Li
authors.affiliation: Liver Fibrosis Diagnosis and Treatment Center, 302 Military Hospital of China Beijing 100039, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Shao
authors.affiliation: Liver Fibrosis Diagnosis and Treatment Center, 302 Military Hospital of China Beijing 100039, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Ji
authors.affiliation: Liver Fibrosis Diagnosis and Treatment Center, 302 Military Hospital of China Beijing 100039, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Li
authors.affiliation: Liver Fibrosis Diagnosis and Treatment Center, 302 Military Hospital of China Beijing 100039, China.
authors.forename: Guofeng
authors.initials: G
authors.lastname: Chen
mesh_terms: D000328:Adult; D044466:Asian People / Q000235:genetics*; D016022:Case-Control Studies; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D019694:Hepatitis B, Chronic / Q000235:genetics*; D006801:Humans; D018960:Hyaluronan Receptors / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D055815:Young Adult
chemical_list: C497483:CD44 protein, human; D018960:Hyaluronan Receptors
keywords: CD44; chronic hepatitis B virus infection; polymorphism