title: Identification of new 18S rRNA strains of Babesia canis isolated from dogs with subclinical babesiosis.
abstract: In this study, we used PCR to detect and characterize B. canis from naturally infected dogs in Poland with subclinical babesiosis by amplifying and sequencing a portion of the 18S ribosomal RNA (rRNA) gene. Venous blood samples were collected from ten dogs with subclinical babesiosis. A 559-bp fragment of the B. canis 18S rRNA gene was amplified by PCR. Sequencing of the PCR products led to the identification of a new variant of Babesia canis, differing from the previously detected protozoa genotypes (18S rRNA-A and 18S rRNA-B) with nucleotide substitutions in positions 150 and 151 of the tested gene fragment. The results indicate the emergence within the Polish territory of a new, previously unencountered Babesia canis genotype responsible for the development of subclinical babesiosis.
authors.forename: P
authors.initials: P
authors.lastname: Łyp
authors.forename: Ł
authors.initials: Ł
authors.lastname: Adaszek
authors.forename: B
authors.initials: B
authors.lastname: Furmaga
authors.forename: S
authors.initials: S
authors.lastname: Winiarczyk
mesh_terms: D000818:Animals; D001403:Babesia / Q000145:classification / Q000235:genetics*; D001404:Babesiosis / Q000469:parasitology*; D004283:Dog Diseases / Q000469:parasitology*; D004285:Dogs; D005260:Female; D008297:Male; D010802:Phylogeny; D016053:RNA, Protozoan / Q000235:genetics*; D012337:RNA, Ribosomal, 18S / Q000235:genetics*
chemical_list: D016053:RNA, Protozoan; D012337:RNA, Ribosomal, 18S