title: Proliferation and osteogenic differentiation of human mesenchymal stem cells on zirconia and titanium with different surface topography.
abstract: The purpose of this study was to elucidate behavior of human mesenchymal stem cells (hMSCs) on yttria stabilized tetragonal zirconia polycrystals (TZP) and commercial pure titanium (CpTi) with different surface topography. Mirror-polished (MS), sandblasted with 150-μm alumina (SB150) and SB150 acid-etched (SB150E) were prepared on TZP and CpTi. Proliferation, osteogenic differentiation of hMSCs was evaluated. The scanning electron microscopy showed that micro- and nano-topographies were created on both TZP and CpTi SB150E surfaces. The proliferation ability, ALP activity, expression of Runx2 on the both SB150E specimens was significantly higher than those on the other specimens. These results suggested that creation of micro- and nano-topographies on TZP and CpTi by blast and acid-etching may offer a promising method for enhancing the proliferation and differentiation of hMSCs in clinical application.
authors.affiliation: Division of Oral Implants Research, Oral Health Science Center, Tokyo Dental College.
authors.forename: Tomoki
authors.initials: T
authors.lastname: Hirano
authors.forename: Hodaka
authors.initials: H
authors.lastname: Sasaki
authors.forename: Shinya
authors.initials: S
authors.lastname: Honma
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Furuya
authors.forename: Tadashi
authors.initials: T
authors.lastname: Miura
authors.forename: Yasutomo
authors.initials: Y
authors.lastname: Yajima
authors.forename: Masao
authors.initials: M
authors.lastname: Yoshinari
mesh_terms: D000134:Acid Etching, Dental; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D006801:Humans; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008855:Microscopy, Electron, Scanning; D010012:Osteogenesis; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*; D015019:Yttrium / Q000737:chemistry; D015040:Zirconium / Q000737:chemistry*
chemical_list: D015019:Yttrium; D015040:Zirconium; D014025:Titanium; C028541:zirconium oxide; C091417:yttria