title: Confluentibacter lentusgen. nov., sp. nov., isolated from the junction between the ocean and a freshwater lake.
abstract: A Gram-stain-negative, non-flagellated, non-gliding, aerobic and rod-shaped bacterium, designated HJM-3T, was isolated from the place where the ocean and a freshwater lake meet at Hwajinpo on the East Sea, South Korea, and subjected to a polyphasic taxonomic study. The novel strain grew optimally at 30-35 °C, at pH 7.0-7.5 and in the presence of 1.0-2.0 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain HJM-3Tclustered with the type strain ofYeosuana aromativoransand the proposed type strain ofMariniflexile ostreae. Strain HJM-3Texhibited the highest 16S rRNA gene sequence similarity values (each 95.1 %) to these representative strains ofY. aromativoransandM. ostreae, and sequence similarities of less than 94.9 % to the type strains of other recognized species. Strain HJM-3Tcontained menaquinone-6 as the predominant isoprenoid quinone, and iso-C15 : 0, iso-C15 : 1G and iso-C17 : 03-OH as the major fatty acids. The major polar lipids detected in strain HJM-3Twere phosphatidylethanolamine and one unidentified lipid. The DNA G+C content of strain HJM-3Twas 34.7 mol%. The fatty acid and polar lipid profiles and DNA G+C content of strain HJM-3Tcould be distinguished from those of the phylogenetically related taxa. On the basis of the phylogenetic, chemotaxonomic and other phenotypic properties, strain HJM-3Tis considered to represent a novel genus and species within the familyFlavobacteriaceae, for which the nameConfluentibacter lentusgen. nov., sp. nov., is proposed. The type strain is HJM-3T( = KCTC 42777T= NBRC 111588T).
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
authors.forename: Sooyeon
authors.initials: S
authors.lastname: Park
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
authors.forename: Sona
authors.initials: S
authors.lastname: Kim
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.|University of Science and Technology (UST), 113 Gwahak-ro, Yuseong-gu, Daejeon, Republic of Korea.
authors.forename: Yong-Taek
authors.initials: YT
authors.lastname: Jung
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
authors.forename: Jin-Min
authors.initials: JM
authors.lastname: Park
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
authors.forename: Jung-Hoon
authors.initials: JH
authors.lastname: Yoon