title: Does methyl jasmonate modify the oxidative stress response in Phaseolus coccineus treated with Cu?
abstract: The contribution of methyl jasmonate (MJ) as a signal molecule able to take part in the defense mechanism against copper (Cu)-imposed oxidative stress was studied in the leaves and roots of runner bean (Phaseolus coccineus) plants. Roots of plants cultivated hydroponically were preincubated in MJ (10µM) for 1h or 24h and subsequently exposed to Cu (50µM) for 5h (short-term experiment) or 5 days (long-term experiment). Enzymatic (activity of superoxide dismutase, SOD; catalase, CAT; ascorbate peroxidase, APX; guaiacol peroxidase, POX) and non-enzymatic (accumulation of malondialdehyde, MDA; homoglutathione, hGSH; proline; anthocyanins; low molecular weight organic acids, LMWOAs) responses were determined in the leaves and roots. The antioxidative defense mechanism was significantly activated after Cu supplementation. In most cases, activities of ROS (reactive oxygen species) scavenging enzymes like SOD, CAT, APX, POX, as well as MDA, hGSH and proline concentrations increased following Cu exposure. MJ showed a time-dependent effect on antioxidative enzymes activity. In the short-term experiment, MJ elevated CAT, APX and POX activities in the roots, and POX activity in the leaves of non-Cu-treated plants. In the long-term experiment, MJ not only decreased POX and partially CAT activity in the roots, but also increased the MDA level and partially CAT activity in the leaves of the control plants. In Cu-treated plants, MJ reduced APX, but elevated POX activity in the leaves after 5-h exposure. After 5-day-Cu treatment, MJ inhibited POX activity in the leaves and mainly reduced SOD and CAT activities in the roots. Moreover, in the long-term experiment, MJ reduced tartrate and pyruvate in the leaves of Cu-stressed plants, but mostly elevated tartrate and malate in the roots comparing with Cu alone treatment. MJ alone and under Cu excess did not alter accumulation of MDA, hGSH and proline comparing with Cu alone, but partially elevated anthocyanin concentration. The results indicated that MJ was both partially potent in modifying the antioxidative enzymes activity and metabolites accumulation in non-stress and Cu-stress conditions.
authors.affiliation: Department of Plant Physiology, Maria Curie-Skłodowska University, Akademicka 19, 20-033 Lublin, Poland. Electronic address: agnieszka.hanaka@umcs.pl.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Hanaka
authors.affiliation: Department of Plant Physiology, Maria Curie-Skłodowska University, Akademicka 19, 20-033 Lublin, Poland.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Wójcik
authors.affiliation: Department of Plant Physiology, Maria Curie-Skłodowska University, Akademicka 19, 20-033 Lublin, Poland.
authors.forename: Sławomir
authors.initials: S
authors.lastname: Dresler
authors.affiliation: Department of Cell Biology, Maria Curie-Skłodowska University, Akademicka 19, 20-033 Lublin, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Mroczek-Zdyrska
authors.affiliation: Department of Plant Physiology, Maria Curie-Skłodowska University, Akademicka 19, 20-033 Lublin, Poland.
authors.forename: Waldemar
authors.initials: W
authors.lastname: Maksymiec
mesh_terms: D000085:Acetates / Q000494:pharmacology*; D000975:Antioxidants / Q000378:metabolism*; D060387:Ascorbate Peroxidases / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D003300:Copper / Q000378:metabolism / Q000633:toxicity*; D003517:Cyclopentanes / Q000494:pharmacology*; D005978:Glutathione / Q000031:analogs & derivatives / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects*; D054883:Oxylipins / Q000494:pharmacology*; D009195:Peroxidase / Q000378:metabolism; D027805:Phaseolus / Q000187:drug effects* / Q000378:metabolism; D018515:Plant Leaves / Q000187:drug effects; D018517:Plant Roots / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000085:Acetates; D000975:Antioxidants; D003517:Cyclopentanes; D054883:Oxylipins; D017382:Reactive Oxygen Species; C402172:homoglutathione; D008315:Malondialdehyde; D003300:Copper; C072239:methyl jasmonate; D006861:Hydrogen Peroxide; C121048:guaiacol peroxidase; D060387:Ascorbate Peroxidases; D002374:Catalase; D009195:Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione
keywords: Antioxidative defense; Antioxidative enzymes; Copper; Jasmonate; Oxidative stress; Phaseolus coccineus