title: DNA Superresolution Structure of Reed-Sternberg Cells Differs Between Long-Lasting Remission Versus Relapsing Hodgkin's Lymphoma Patients.
abstract: Recent developments in microscopy have led to superresolution microscopy images of cells. Structured illumination microscopy was used before to reveal new details in the DNA structure and the structure of the DNA-free space in the DAPI-stained cell nuclei of the Hodgkin's lymphoma HDLM-2 cell line. This study extends this technology to primary pre-treatment classical Hodgkin's lymphoma samples of ten patients. Significant differences in both the DNA structure and the structure of the DNA-free space were detected between lymphocytes and malignant cells. Both types of structures were similar for lymphocytes of different patients. When the patients were un-blinded and grouped based on their clinical outcome, either non-relapsed or relapsed, a significant difference in the DNA structure of their Reed-Sternberg (RS) cells was found. Since, RS cells develop from mono-nucleated Hodgkin (H) cells, these data suggest distinct architectural restructuring of nuclei during RS cell formation in patients going to long-lasting remission versus relapse. J. Cell. Biochem. 117: 1633-1637, 2016. © 2015 Wiley Periodicals, Inc.
authors.affiliation: Manitoba Institute of Cell Biology, CancerCare Manitoba, University of Manitoba, Winnipeg, Manitoba, Canada.
authors.forename: Christiaan H
authors.initials: CH
authors.lastname: Righolt
authors.affiliation: Department of Medicine, Jewish General Hospital, McGill University, Montreal, Quebec, Canada.
authors.forename: Hans
authors.initials: H
authors.lastname: Knecht
authors.affiliation: Manitoba Institute of Cell Biology, CancerCare Manitoba, University of Manitoba, Winnipeg, Manitoba, Canada.
authors.forename: Sabine
authors.initials: S
authors.lastname: Mai
mesh_terms: D000328:Adult; D045744:Cell Line, Tumor; D004273:DNA, Neoplasm / Q000378:metabolism*; D005260:Female; D006689:Hodgkin Disease / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009690:Nucleic Acid Conformation; D012008:Recurrence; D016539:Reed-Sternberg Cells / Q000378:metabolism* / Q000473:pathology
chemical_list: D004273:DNA, Neoplasm
keywords: DNA STRUCTURE; HODGKIN'S LYMPHOMA; NUCLEAR ARCHITECTURE; STRUCTURED ILLUMINATION MICROSCOPY