title: Association of I-FABP gene polymorphism and the risk of coronary heart disease.
abstract: OBJECTIVE
  The study aimed to investigate the association between polymorphism of I-FABP gene and coronary heart disease (CHD).
  
  
  METHODS
  225 patients with CHD were randomly recruited into the case group, and 196 healthy elderly volunteers were recruited from Medical Examination Center of our hospital as control. General clinical data were collected and plasma TC, TG, LDL-C, HDL-C levels were measured. Besides, polymerase chain reaction (PCR) and Restriction Fragment Length Polymorphism (RFLP) technology were used to detect the polymorphism of Hha-I enzyme cleavage sites in I-FABP gene in the study population.
  
  
  RESULTS
  Hha-I cleavage sites occurred at codon 54 in exon in the coding sequencing of I-FABP gene in all participants. After cleavage with Hha-I enzyme, the genotypes were identified as wild-type A/A, heterozygous mutant A/T and homozygous mutant T/T. In case group, A/T and T/T genetic carriers had significantly higher levels of TC, TG and LDL-C than A/A carriers (P<0.05). However, in control group, similar differences were not observed (P>0.05). BMI, dietary habits and I-FABP alleles were independent risk factors of CHD.
  
  
  CONCLUSIONS
  The polymorphism of I-FABP gene existed in the study population. And this genetic variation had influence on lipid metabolism, which was associated with the risk of developing CHD. I-FABP gene polymorphism may contribute to the increased genetic susceptibility to CHD.
authors.affiliation: Department of Cardiology, Daping Hospital, The Third Military Medical University Chongqing 400042, China ; Chongqing Institute of Cardiology Chongqing 400042, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Yuan
authors.affiliation: Department of Cardiology, Daping Hospital, The Third Military Medical University Chongqing 400042, China ; Chongqing Institute of Cardiology Chongqing 400042, China.
authors.forename: Changqing
authors.initials: C
authors.lastname: Yu
authors.affiliation: Department of Cardiology, Daping Hospital, The Third Military Medical University Chongqing 400042, China ; Chongqing Institute of Cardiology Chongqing 400042, China.
authors.forename: Chunyu
authors.initials: C
authors.lastname: Zeng
keywords: Fatty acid binding proteins; PCR; alleles; coronary heart disease; dyslipidemia; risk factors