title: Diterpene alkaloids and diterpenes from Spiraea japonica and their anti-tobacco mosaic virus activity.
abstract: Five new naturally occurring natural products, including two atisine-type diterpene alkaloids (1 and 2), two atisane-type diterpenes (3 and 4), and a new natural product spiramine C2 (5), along with nine known ones (6-14), were isolated from the ethanolic extracts of the whole plant of Spiraea japonica var. acuminata Franch. Their structures were elucidated by extensive spectroscopic analysis. The anti-tobacco mosaic virus (TMV) activities of all the compounds were evaluated by the conventional half-leaf method. Six compounds (2, 3, 6, 7, 11, and 12) exhibited moderate activities at 100 μg/mL with inhibition rates in the range of 69.4-92.9%, which were higher than that of the positive control, ningnanmycin. Their preliminary structure-activity relationships were also discussed.
authors.affiliation: School of Chemistry and Chemical Engineering, Guizhou University, Guiyang 550025, People's Republic of China; The Key Laboratory of Chemistry for Natural Product of Guizhou Province and Chinese Academy of Science, Guiyang 550002, People's Republic of China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Ma
authors.affiliation: The Key Laboratory of Chemistry for Natural Product of Guizhou Province and Chinese Academy of Science, Guiyang 550002, People's Republic of China.
authors.forename: Xin-Ying
authors.initials: XY
authors.lastname: Mao
authors.affiliation: The Key Laboratory of Chemistry for Natural Product of Guizhou Province and Chinese Academy of Science, Guiyang 550002, People's Republic of China.
authors.forename: Lie-Jun
authors.initials: LJ
authors.lastname: Huang
authors.affiliation: School of Chemistry and Chemical Engineering, Guizhou University, Guiyang 550025, People's Republic of China; The Key Laboratory of Chemistry for Natural Product of Guizhou Province and Chinese Academy of Science, Guiyang 550002, People's Republic of China.
authors.forename: Yi-Min
authors.initials: YM
authors.lastname: Fan
authors.affiliation: The Key Laboratory of Chemistry for Natural Product of Guizhou Province and Chinese Academy of Science, Guiyang 550002, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Gu
authors.affiliation: The Key Laboratory of Chemistry for Natural Product of Guizhou Province and Chinese Academy of Science, Guiyang 550002, People's Republic of China.
authors.forename: Chen
authors.initials: C
authors.lastname: Yan
authors.affiliation: The Key Laboratory of Chemistry for Natural Product of Guizhou Province and Chinese Academy of Science, Guiyang 550002, People's Republic of China.
authors.forename: Tao
authors.initials: T
authors.lastname: Huang
authors.affiliation: The Key Laboratory of Chemistry for Natural Product of Guizhou Province and Chinese Academy of Science, Guiyang 550002, People's Republic of China.
authors.forename: Jian-Xin
authors.initials: JX
authors.lastname: Zhang
authors.affiliation: The Key Laboratory of Chemistry for Natural Product of Guizhou Province and Chinese Academy of Science, Guiyang 550002, People's Republic of China. Electronic address: yuanchunmao01@126.com.
authors.forename: Chun-Mao
authors.initials: CM
authors.lastname: Yuan
authors.affiliation: The Key Laboratory of Chemistry for Natural Product of Guizhou Province and Chinese Academy of Science, Guiyang 550002, People's Republic of China. Electronic address: haoxj@mail.kib.ac.cn.
authors.forename: Xiao-Jiang
authors.initials: XJ
authors.lastname: Hao
mesh_terms: D000470:Alkaloids / Q000737:chemistry* / Q000302:isolation & purification; D000998:Antiviral Agents / Q000737:chemistry* / Q000302:isolation & purification; D004224:Diterpenes / Q000737:chemistry* / Q000302:isolation & purification; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry; D031992:Spiraea / Q000737:chemistry*; D013329:Structure-Activity Relationship; D014027:Tobacco Mosaic Virus / Q000187:drug effects*
chemical_list: D000470:Alkaloids; D000998:Antiviral Agents; D004224:Diterpenes; D010936:Plant Extracts
keywords: Anti-TMV activities; Atisane-type diterpenes; Atisine-type diterpene alkaloids; Spiraea japonica