title: Transplantation of olfactory ensheathing cells promotes partial recovery in rats with experimental autoimmune encephalomyelitis.
abstract: OBJECTIVE
  This study was to investigate the efficacy of olfactory ensheathing cell (OEC) transplantation on experimental autoimmune encephalomyelitis (EAE).
  
  
  METHODS
  EAE models were established by guinea pig spinal cord homogenate (GPSCH) immunization in Lewis rats. OECs were purified and cultured from the olfactory nerve layer of SD rats, and then transplanted to the EAE models through the vena caudalis (Group A) or into the lateral cerebral ventricle (Group B). Neurological function scores and body weights were daily recorded following transplantation, and histological analysis was performed to assess the pathological changes in EAE rats.
  
  
  RESULTS
  Cultured cells mainly exhibited bipolar or tripolar morphology, and the majority of these cells were positive for NGFR p75 staining. Neurological function scoring and the body weight measurement showed that, OEC transplantation could significantly improve the performance of EAE rats, and similar results were observed for the transplantation through the vena caudalis and into the lateral cerebral ventricle. Moreover, the transplanted OECs accumulated to the lesions in the brains of EAE rats, in spite of the different transplantation approaches. However, no significant differences in histopathology (HE and LFB staining) were observed between the OEC-transplanted groups and the control group.
  
  
  CONCLUSIONS
  OEC transplantation could exert beneficial effects in the treatment of EAE, no matter which the cells were transplanted through the vena caudalis or into the lateral cerebral ventricle. Our findings might provide evidence for the clinical treatment of multiple sclerosis with cell transplantation.
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Wenzhou Medical University Wenzhou 32500, Zhejiang, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Wenzhou Medical University Wenzhou 32500, Zhejiang, China.
authors.forename: Weian
authors.initials: W
authors.lastname: Chen
authors.affiliation: Department of Pediatric Orthopedics, The Second Affiliated Hospital of Wenzhou Medical University Wenzhou 32500, Zhejiang, China.
authors.forename: Yu'an
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Wenzhou Medical University Wenzhou 32500, Zhejiang, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Wenzhou Medical University Wenzhou 32500, Zhejiang, China.
authors.forename: Zhangna
authors.initials: Z
authors.lastname: Ding
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Wenzhou Medical University Wenzhou 32500, Zhejiang, China.
authors.forename: Dehao
authors.initials: D
authors.lastname: Yang
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Wenzhou Medical University Wenzhou 32500, Zhejiang, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D001921:Brain / Q000276:immunology / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology; D017690:Cell Transplantation / Q000379:methods*; D002478:Cells, Cultured; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology / Q000601:surgery*; D005260:Female; D005455:Fluorescent Antibody Technique; D005620:Freund's Adjuvant; D006168:Guinea Pigs; D064593:Heterografts; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009457:Neuroglia / Q000378:metabolism / Q000637:transplantation*; D009460:Neurologic Examination; D009832:Olfactory Nerve / Q000166:cytology / Q000378:metabolism / Q000637:transplantation*; D037342:Pertussis Toxin; D010641:Phenotype; D011917:Rats, Inbred Lew; D017207:Rats, Sprague-Dawley; D017978:Receptors, Growth Factor / Q000378:metabolism; D020127:Recovery of Function; D013116:Spinal Cord / Q000276:immunology / Q000637:transplantation; D013997:Time Factors
chemical_list: D015415:Biomarkers; D009419:Nerve Tissue Proteins; D017978:Receptors, Growth Factor; C068313:Ngfr protein, rat; D005620:Freund's Adjuvant; D037342:Pertussis Toxin
keywords: Multiple sclerosis (MS); cell transplantation; experimental autoimmune encephalomyelitis (EAE); olfactory ensheathing cells (OECs)