title: Systematic optimization of fed-batch simultaneous saccharification and fermentation at high-solid loading based on enzymatic hydrolysis and dynamic metabolic modeling of Saccharomyces cerevisiae.
abstract: An integrative simultaneous saccharification and fermentation (SSF) modeling is a useful guiding tool for rapid process optimization to meet the techno-economic requirement of industrial-scale lignocellulosic ethanol production. In this work, we have developed the SSF model composing of a metabolic network of a Saccharomyces cerevisiae cell associated with fermentation kinetics and enzyme hydrolysis model to quantitatively capture dynamic responses of yeast cell growth and fermentation during SSF. By using model-based design of feeding profiles for substrate and yeast cell in the fed-batch SSF process, an efficient ethanol production with high titer of up to 65 g/L and high yield of 85 % of theoretical yield was accomplished. The ethanol titer and productivity was increased by 47 and 41 %, correspondingly, in optimized fed-batch SSF as compared to batch process. The developed integrative SSF model is, therefore, considered as a promising approach for systematic design of economical and sustainable SSF bioprocessing of lignocellulose.
authors.affiliation: National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), 113 Thailand Science Park Phahonyothin Road, Klong Nueng, Klong Luang, Pathum Thani, 12120, Thailand. pornkamol.unr@biotec.or.th.
authors.forename: Pornkamol
authors.initials: P
authors.lastname: Unrean
authors.affiliation: National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), 113 Thailand Science Park Phahonyothin Road, Klong Nueng, Klong Luang, Pathum Thani, 12120, Thailand.
authors.forename: Sutamat
authors.initials: S
authors.lastname: Khajeeram
authors.affiliation: National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), 113 Thailand Science Park Phahonyothin Road, Klong Nueng, Klong Luang, Pathum Thani, 12120, Thailand.
authors.forename: Kobkul
authors.initials: K
authors.lastname: Laoteng
mesh_terms: D061249:Batch Cell Culture Techniques; D050260:Carbohydrate Metabolism; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D006867:Hydrolases / Q000378:metabolism*; D006868:Hydrolysis; D008031:Lignin / Q000378:metabolism*; D008954:Models, Biological; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism*
chemical_list: C036909:lignocellulose; D000431:Ethanol; D008031:Lignin; D006867:Hydrolases
keywords: Dynamic flux balance analysis; Lignocellulosic ethanol; SSF model; Systematic fed-batch bioprocess design