title: The contribution of translesion synthesis polymerases on geminiviral replication.
abstract: Geminiviruses multiply primarily in the plant phloem, but never in meristems. Their Rep protein can activate DNA synthesis in differentiated cells. However, when their single-stranded DNA is injected into the phloem by insects, no Rep is present for inducing initial complementary strand replication. Considering a contribution of translesion synthesis (TLS) polymerases in plants, four of them (Polη, Polζ, Polκ, Rev1) are highly and constitutively expressed in differentiated tissues like the phloem. Two geminiviruses (Euphorbia yellow mosaic virus, Cleome leaf crumple virus), inoculated either biolistically or by whiteflies, replicated in Arabidopsis thaliana mutant lines of these genes to the same extent as in wild type plants. Comparative deep sequencing of geminiviral DNAs, however, showed a high exchange rate (10(-4)-10(-3)) similar to the phylogenetic variation described before and a significant difference in nucleotide substation rates if Polη and Polζ were absent, with a differential response to the viral DNA components.
authors.affiliation: Institute of Biomaterials and Biomolecular Systems, Department of Molecular Biology and Plant Virology, University of Stuttgart, Pfaffenwaldring 57, D-70550 Stuttgart, Germany.
authors.forename: Kathrin S
authors.initials: KS
authors.lastname: Richter
authors.affiliation: Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Messeweg 11-12, D-38104 Braunschweig, Germany.
authors.forename: Monika
authors.initials: M
authors.lastname: Götz
authors.affiliation: Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Messeweg 11-12, D-38104 Braunschweig, Germany.
authors.forename: Stephan
authors.initials: S
authors.lastname: Winter
authors.affiliation: Institute of Biomaterials and Biomolecular Systems, Department of Molecular Biology and Plant Virology, University of Stuttgart, Pfaffenwaldring 57, D-70550 Stuttgart, Germany. Electronic address: holger.jeske@bio.uni-stuttgart.de.
authors.forename: Holger
authors.initials: H
authors.lastname: Jeske
mesh_terms: D017360:Arabidopsis / Q000821:virology*; D053839:Begomovirus / Q000502:physiology*; D054884:Host-Pathogen Interactions; D009713:Nucleotidyltransferases / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism*; D014779:Virus Replication
chemical_list: D010940:Plant Proteins; D009713:Nucleotidyltransferases
keywords: Complementary strand replication; DNA damage tolerance; Geminivirus evolution; Mutation rate; Polymerase ζ; Polymerase η