title: Application of a high-throughput, parallel HPLC system for quantitative chiral analysis of pantoprazole.
abstract: BACKGROUND
  Chromatographic separation of enantiomers is considered a task in analytical chemistry particularly for high sample throughput. This paper describes a high-throughput parallel HPLC-MS/MS method for the determination of pantoprazole enantiomers.
  
  
  RESULTS
  Baseline separation of pantoprazole enantiomers was achieved on a Chiralcel OZ-RH column in a run time of 4.5 min. Assays for enantiomers were linear with satisfactory intra- and inter-day precision and accuracy. The assay was suitable for high-throughput analysis as shown by its successful application to a chiral PK study in beagle dog.
  
  
  CONCLUSIONS
  A high-throughput parallel HPLC-MS/MS assay for pantoprazole has been developed and validated. This method provides nearly twofold increased sample throughput, and was shown to be suitable for application in PK studies.
authors.affiliation: College of Life Sciences, Jilin University, Changchun 130012, PR China.
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
authors.affiliation: School of Pharmaceutical Sciences, Jilin University, Changchun 130012, PR China.
authors.forename: Yantong
authors.initials: Y
authors.lastname: Sun
authors.affiliation: College of Life Sciences, Jilin University, Changchun 130012, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Research Center for Drug Metabolism, Jilin University, Changchun 130012, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Guo
authors.affiliation: College of Life Sciences, Jilin University, Changchun 130012, PR China.
authors.forename: Mi
authors.initials: M
authors.lastname: Zheng
authors.affiliation: Research Center for Drug Metabolism, Jilin University, Changchun 130012, PR China.
authors.forename: Weiping
authors.initials: W
authors.lastname: Zhang
authors.affiliation: School of Pharmacy, University of Otago, PO Box 56, Dunedin, New Zealand.
authors.forename: J Paul
authors.initials: JP
authors.lastname: Fawcett
authors.affiliation: Research Center for Drug Metabolism, Jilin University, Changchun 130012, PR China.
authors.forename: Jingkai
authors.initials: J
authors.lastname: Gu
mesh_terms: D053799:2-Pyridinylmethylsulfinylbenzimidazoles / Q000494:pharmacology*; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004285:Dogs; D000077402:Pantoprazole; D013237:Stereoisomerism
chemical_list: D053799:2-Pyridinylmethylsulfinylbenzimidazoles; D000077402:Pantoprazole