title: Isolation and characterization of heavy polycyclic aromatic hydrocarbon-degrading bacteria adapted to electrokinetic conditions.
abstract: Polycyclic aromatic hydrocarbon (PAH)-degrading bacteria capable of growing under electrokinetic conditions were isolated using an adjusted acclimation and enrichment procedure based on soil contaminated with heavy PAHs in the presence of an electric field. Their ability to degrade heavy PAHs under an electric field was individually investigated in artificially contaminated soils. The results showed that strains PB4 (Pseudomonas fluorescens) and FB6 (Kocuria sp.) were the most efficient heavy PAH degraders under electrokinetic conditions. They were re-inoculated into a polluted soil from an industrial site with a PAH concentration of 184.95 mg kg(-1). Compared to the experiments without an electric field, the degradation capability of Pseudomonas fluorescens and Kocuria sp. was enhanced in the industrially polluted soil under electrokinetic conditions. The degradation extents of total PAHs were increased by 15.4 and 14.0% in the electrokinetic PB4 and FB6 experiments (PB4 + EK and FB6 + EK) relative to the PB4 and FB6 experiments without electrokinetic conditions (PB4 and FB6), respectively. These results indicated that P. fluorescens and Kocuria sp. could efficiently degrade heavy PAHs under electrokinetic conditions and have the potential to be used for the electro-bioremediation of PAH-contaminated soil, especially if the soil is contaminated with heavy PAHs.
authors.affiliation: Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, 110016, China. fmli2010@hotmail.com.
authors.forename: Fengmei
authors.initials: F
authors.lastname: Li
authors.affiliation: Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, 110016, China. shuhaiguo@iae.ac.cn.
authors.forename: Shuhai
authors.initials: S
authors.lastname: Guo
authors.affiliation: KWR Watercycle Research Institute, 3433 PE, Nieuwegein, The Netherlands.
authors.forename: Niels
authors.initials: N
authors.lastname: Hartog
authors.affiliation: Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, 110016, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: Shenyang University, Shenyang, 110014, China.
authors.forename: Xuelian
authors.initials: X
authors.lastname: Yang
mesh_terms: D001419:Bacteria / Q000302:isolation & purification* / Q000378:metabolism*; D001673:Biodegradation, Environmental; D004560:Electricity; D007220:Industrial Waste; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis* / Q000378:metabolism; D011551:Pseudomonas fluorescens / Q000378:metabolism; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000032:analysis* / Q000378:metabolism
chemical_list: D007220:Industrial Waste; D011084:Polycyclic Aromatic Hydrocarbons; D012989:Soil Pollutants
keywords: Degrading bacteria; Electric field; Heavy polycyclic aromatic hydrocarbons; Industrially polluted soil