title: 2-DE Gel Analysis: The Spot Detection.
abstract: The abundance of different proteins on a 2-DE gel is reflected by the shape, size, and intensity of the corresponding spots. Protein quantitation requires the conversion of an analog gel image into digital data, resulting into a catalog of individual spots listed as x, y positions, shape parameters, and quantitative values. So, it is possible to carry out objective comparisons of equivalent spots on different gels, determining whether a particular protein is more or less abundant in one sample compared with another. Unfortunately, spots on protein gels are not uniform in shape, size, or density, and detection, quantitation, and comparison can be challenging without intervention. Once a processed image is available, a number of different algorithms can be applied to detect and quantitate individual spots.
authors.affiliation: DiSIT-Dipartimento di Scienze e Innovazione Tecnologica, University of Piemonte Orientale, viale Teresa Michel, 11, Alessandria, 15121, Italy. simona.martinotti@uniupo.it.
authors.forename: Simona
authors.initials: S
authors.lastname: Martinotti
authors.affiliation: DiSIT-Dipartimento di Scienze e Innovazione Tecnologica, University of Piemonte Orientale, viale Teresa Michel, 11, Alessandria, 15121, Italy.
authors.forename: Elia
authors.initials: E
authors.lastname: Ranzato
mesh_terms: D000465:Algorithms; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D007091:Image Processing, Computer-Assisted; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification*; D040901:Proteomics / Q000379:methods*; D012984:Software
chemical_list: D011506:Proteins
keywords: Image analysis; Spot detection; Spot segmentation