title: Conventional Matrices Loaded Onto a Graphene Layer Enhances MALDI-TOF/TOF Signal: Its Application to Improve Detection of Phosphorylated Peptides.
abstract: This is the first study where graphene is used as a MALDI adjuvant in combination with the traditional matrix α-cyano-4-hydroxycinnamic acid (CHCA) to improve the signal intensity of peptide samples. Use of this amended matrix not only leads to increased signals but also to a higher number of peaks detected in complex samples. Additionally, the use of graphene has a stabilizing effect that can also be exploited to improve the detection of easily cleavable molecules. Graphical Abstract ᅟ.
authors.affiliation: Proteomics Unit of the Supercomputing and Bioinnovation Center of the University of Málaga, Parque Tecnológico de Andalucía, Málaga, Spain. acrge1@gmail.com.
authors.forename: Carlos E
authors.initials: CE
authors.lastname: Rodríguez
authors.affiliation: Proteomics Unit of the Supercomputing and Bioinnovation Center of the University of Málaga, Parque Tecnológico de Andalucía, Málaga, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: Palacios
authors.affiliation: Department of Molecular Biology and Biochemistry, University of Malaga, Málaga, Spain.|Unit 741 of Centro de Investigación en Red en Enfermedades Raras (CIBERER-ISCIII), Malaga, Spain.
authors.forename: Ignacio
authors.initials: I
authors.lastname: Fajardo
authors.affiliation: Department of Molecular Biology and Biochemistry, University of Malaga, Málaga, Spain.|Unit 741 of Centro de Investigación en Red en Enfermedades Raras (CIBERER-ISCIII), Malaga, Spain.
authors.forename: José Luis
authors.initials: JL
authors.lastname: Urdiales
authors.affiliation: Therapeutic Nanosystem, Andalusian Center for Nanomedicine and Biotechnology (BIONAND), Parque Tecnológico de Andalucía, Campanillas, Málaga, Spain.
authors.forename: Xavier
authors.initials: X
authors.lastname: Le Guével
authors.affiliation: Department of Molecular Biology and Biochemistry, University of Malaga, Málaga, Spain.|Laboratorio de Oncología Molecular, Servicio de Oncología Médica, Instituto de Biomedicina de Málaga (IBIMA), Hospital Universitario Virgen de la Victoria, Málaga, Spain.
authors.forename: José
authors.initials: J
authors.lastname: Lozano
authors.affiliation: Department of Molecular Biology and Biochemistry, University of Malaga, Málaga, Spain.|Unit 741 of Centro de Investigación en Red en Enfermedades Raras (CIBERER-ISCIII), Malaga, Spain.
authors.forename: Francisca
authors.initials: F
authors.lastname: Sánchez-Jiménez
mesh_terms: D006108:Graphite; D010455:Peptides / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism*; D010750:Phosphoproteins / Q000032:analysis / Q000378:metabolism; D010766:Phosphorylation; D012815:Signal Processing, Computer-Assisted; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D010455:Peptides; D010750:Phosphoproteins; D006108:Graphite
keywords: Graphene; MALDI-TOF/TOF; Phosphorylated peptides