title: Gastrointestinal digested Sambucus nigra L. fruit extract protects in vitro cultured human colon cells against oxidative stress.
abstract: Elderberry (EDB) Sambucus nigra L. is one of the oldest medicinal plants which is useful for therapeutic and nutritional purposes due to a large amount of biologically active constituents, including compounds with a high antioxidant capacity. The present study focused on the antioxidant potential of the colon-available EDB fruit extract, derived from the artificial gastrointestinal tract, with regard to human colonic mucosa cells cultured in vitro. Despite the significant loss of EDB bioactive compounds due to the digestion process, the colon-digested extract was able to reduce the excessive intracellular ROS production (22%) and oxidative DNA damage (46%) in the colon cells at a dose of 1 mg of freeze-dried EDB powder/ml. Moreover, the colon-digested EDB extract inhibited oxidant-induced mutagenicity (26%) in the Salmonella typhimurium TA102 strain, as determined by the Ames test. In conclusion, the current in vitro study confirmed that the fruits of S. nigra are capable of protecting colonic cells against the detrimental effects of oxidative stress.
authors.affiliation: Department of Biotechnology and Food Microbiology, Poznań University of Life Sciences, Wojska Polskiego 48, 60-627 Poznań, Poland. Electronic address: aolejnik@up.poznan.pl.
authors.forename: Anna
authors.initials: A
authors.lastname: Olejnik
authors.affiliation: Department of Biotechnology and Food Microbiology, Poznań University of Life Sciences, Wojska Polskiego 48, 60-627 Poznań, Poland.
authors.forename: Mariola
authors.initials: M
authors.lastname: Olkowicz
authors.affiliation: Department of Biotechnology and Food Microbiology, Poznań University of Life Sciences, Wojska Polskiego 48, 60-627 Poznań, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Kowalska
authors.affiliation: Department of Biotechnology and Food Microbiology, Poznań University of Life Sciences, Wojska Polskiego 48, 60-627 Poznań, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Rychlik
authors.affiliation: Department of Biotechnology and Food Microbiology, Poznań University of Life Sciences, Wojska Polskiego 48, 60-627 Poznań, Poland.
authors.forename: Radosław
authors.initials: R
authors.lastname: Dembczyński
authors.affiliation: Department of Biotechnology and Food Microbiology, Poznań University of Life Sciences, Wojska Polskiego 48, 60-627 Poznań, Poland.
authors.forename: Kamila
authors.initials: K
authors.lastname: Myszka
authors.affiliation: Department of Biotechnology and Food Microbiology, Poznań University of Life Sciences, Wojska Polskiego 48, 60-627 Poznań, Poland.
authors.forename: Wojciech
authors.initials: W
authors.lastname: Juzwa
authors.affiliation: Department of Biotechnology and Food Microbiology, Poznań University of Life Sciences, Wojska Polskiego 48, 60-627 Poznań, Poland.
authors.forename: Wojciech
authors.initials: W
authors.lastname: Białas
authors.affiliation: INCELL Corporation, LLC, San Antonio, TX 78249, United States.
authors.forename: Mary Pat
authors.initials: MP
authors.lastname: Moyer
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D002460:Cell Line; D003106:Colon / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D004249:DNA Damage / Q000187:drug effects; D005638:Fruit / Q000737:chemistry*; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity; D007413:Intestinal Mucosa / Q000187:drug effects; D009153:Mutagens / Q000633:toxicity; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000032:analysis / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D012486:Salmonella typhimurium / Q000187:drug effects / Q000378:metabolism; D027924:Sambucus nigra / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D009153:Mutagens; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide
keywords: Antimutagenic activity; Antioxidant capacity; Colon cells; DNA damage; Elderberry; ROS generation