title: Validating the importance of two acetylcholinesterases in insecticide sensitivities by RNAi in Pardosa pseudoannulata, an important predatory enemy against several insect pests.
abstract: The pond wolf spider (Pardosa pseudoannulata) is an important predatory enemy against several insect pests and showed relative different sensitivities to organophosphate and carbamate insecticides compared to insect pests. In our previous studies, two acetylcholinesterases were identified in P. pseudoannulata and played important roles in insecticide sensitivities. In order to understand the contributions of the two acetylcholinesterases to insecticide sensitivities, we firstly employed the RNAi technology in the spider. For a suitable microinjection RNAi method, the injection site, injection volume and interference time were optimized, which then demonstrated that the injection RNAi method was applicable in this spider. With the new RNAi method, it was revealed that both Pp-AChE1 and Pp-AChE2, encoded by genes Ppace1 and Ppace2, were the targets of organophosphate insecticides, but Pp-AChE1 would be more important. In contrast, the carbamate acted selectively on Pp-AChE1. The results showed that Pp-AChE1 was the major catalytic enzyme in P. pseudoannulata and the major target of organophosphate and carbamate insecticides. In a word, an RNAi method was established in the pond wolf spider, which further validated the importance of two acetylcholinesterases in insecticide sensitivities in this spider.
authors.affiliation: Key Laboratory of Integrated Management of Crop Diseases and Pests (Ministry of Education), College of Plant Protection, Nanjing Agricultural University, Weigang 1, Nanjing 210095, China.
authors.forename: Xiangkun
authors.initials: X
authors.lastname: Meng
authors.affiliation: Key Laboratory of Integrated Management of Crop Diseases and Pests (Ministry of Education), College of Plant Protection, Nanjing Agricultural University, Weigang 1, Nanjing 210095, China.
authors.forename: Chunrui
authors.initials: C
authors.lastname: Li
authors.affiliation: Key Laboratory of Integrated Management of Crop Diseases and Pests (Ministry of Education), College of Plant Protection, Nanjing Agricultural University, Weigang 1, Nanjing 210095, China; Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, St. Zhongling 50, Nanjing 210014, China.
authors.forename: Haibo
authors.initials: H
authors.lastname: Bao
authors.affiliation: Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, St. Zhongling 50, Nanjing 210014, China.
authors.forename: Jichao
authors.initials: J
authors.lastname: Fang
authors.affiliation: Key Laboratory of Integrated Management of Crop Diseases and Pests (Ministry of Education), College of Plant Protection, Nanjing Agricultural University, Weigang 1, Nanjing 210095, China. Electronic address: liuzewen@njau.edu.cn.
authors.forename: Zewen
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Key Laboratory of Integrated Management of Crop Diseases and Pests (Ministry of Education), College of Plant Protection, Nanjing Agricultural University, Weigang 1, Nanjing 210095, China. Electronic address: zhangyixi@njau.edu.cn.
authors.forename: Yixi
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000110:Acetylcholinesterase / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D060829:Arthropod Proteins / Q000235:genetics / Q000378:metabolism*; D002219:Carbamates / Q000494:pharmacology; D005821:Genetic Techniques; D007313:Insecta / Q000469:parasitology; D007306:Insecticides / Q000494:pharmacology*; D008958:Models, Molecular; D010755:Organophosphates / Q000494:pharmacology; D034622:RNA Interference; D013112:Spiders / Q000187:drug effects* / Q000201:enzymology* / Q000235:genetics
chemical_list: D060829:Arthropod Proteins; D002219:Carbamates; D007306:Insecticides; D010755:Organophosphates; D000110:Acetylcholinesterase
keywords: Acetylcholinesterase; Insecticide sensitivity; Pardosa pseudoannulata; RNA interference