title: Teichoic acid is the major polysaccharide present in the Listeria monocytogenes biofilm matrix.
abstract: The aim of this study was to characterize the Listeria monocytogenes biofilm and particularly the nature of the carbohydrates in the biofilm extracellular matrix and culture supernatant versus to cell wall carbohydrates. Listeria monocytogenes serotype 1/2a and 4b strains were able to form complex biofilms embedded in an extracellular matrix. The soluble carbohydrates from biofilm extracellular matrix and culture supernatant were identified as teichoic acids, structurally identical to cell wall teichoic acids. In addition, the DSS 1130 BFA2 strain had a serotype 1/2a teichoic acid lacking N-acetyl glucosamine glycosylation due to a mutation in the lmo2550 gene. Consequently, we hypothesized that the extracellular teichoic acids in L. monocytogenes biofilms have the same origin as cell wall teichoic acid.
authors.affiliation: ANSES, Laboratory for Food Safety, Boulevard Bassin Napoléon, F-62200 Boulogne Mur Mer, France.
authors.forename: Thomas
authors.initials: T
authors.lastname: Brauge
authors.affiliation: Université du Littoral-Côte d'Opale, Institut Charles Violette EA 7394, Bassin Napoléon, F-62200 Boulogne-sur-Mer, France.
authors.forename: Irina
authors.initials: I
authors.lastname: Sadovskaya
authors.affiliation: INRA, UMR8207, F-59650 Villeneuve d'Ascq, France.
authors.forename: Christine
authors.initials: C
authors.lastname: Faille
authors.affiliation: INRA, UMR8207, F-59650 Villeneuve d'Ascq, France.
authors.forename: Thierry
authors.initials: T
authors.lastname: Benezech
authors.affiliation: Université Lille 1, CNRS, UMR 8576, UGSF, F-59000 Lille, France.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Maes
authors.affiliation: Université Lille 1, CNRS, UMR 8576, UGSF, F-59000 Lille, France.
authors.forename: Yann
authors.initials: Y
authors.lastname: Guerardel
authors.affiliation: ANSES, Laboratory for Food Safety, Boulevard Bassin Napoléon, F-62200 Boulogne Mur Mer, France graziella.bourdin@anses.fr.
authors.forename: Graziella
authors.initials: G
authors.lastname: Midelet-Bourdin
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D018441:Biofilms; D002473:Cell Wall / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D006031:Glycosylation; D008089:Listeria monocytogenes / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D011135:Polysaccharides, Bacterial / Q000737:chemistry / Q000378:metabolism*; D013682:Teichoic Acids / Q000737:chemistry* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D011135:Polysaccharides, Bacterial; D013682:Teichoic Acids
keywords: Listeria; biofilm; carbohydrate structure; glycosyltransferase; sequencing; teichoic acid