title: Dystroglycan Depletion Impairs Actin-Dependent Functions of Differentiated Kasumi-1 Cells.
abstract: BACKGROUND
  Dystroglycan has recently been characterised in blood tissue cells, as part of the dystrophin glycoprotein complex involved in the differentiation process of neutrophils.
  
  
  OBJECTIVE
  In the present study we have investigated the role of dystroglycan in the human promyelocytic leukemic cell line Kasumi-1 differentiated to macrophage-like cells.
  
  
  METHODS
  We characterised the pattern expression and subcellular distribution of dystroglycans in non-differentiated and differentiated Kasumi-1 cells.
  
  
  RESULTS
  Our results demonstrated by WB and flow cytometer assays that during the differentiation process to macrophages, dystroglycans were down-regulated; these results were confirmed with qRT-PCR assays. Additionally, depletion of dystroglycan by RNAi resulted in altered morphology and reduced properties of differentiated Kasumi-1 cells, including morphology, migration and phagocytic activities although secretion of IL-1β and expression of markers of differentiation are not altered.
  
  
  CONCLUSIONS
  Our findings strongly implicate dystroglycan as a key membrane adhesion protein involved in actin-based structures during the differentiation process in Kasumi-1 cells.
authors.affiliation: Laboratorio de Hematobiología, Escuela Nacional de Medicina y Homeopatía (ENMH), Instituto Politécnico Nacional (IPN), Mexico City, Mexico.
authors.forename: Marco Antonio
authors.initials: MA
authors.lastname: Escárcega-Tame
authors.affiliation: Laboratorio de Hematobiología, Escuela Nacional de Medicina y Homeopatía (ENMH), Instituto Politécnico Nacional (IPN), Mexico City, Mexico.
authors.forename: Ivette
authors.initials: I
authors.lastname: Martínez-Vieyra
authors.affiliation: Laboratorio de Hematobiología, Escuela Nacional de Medicina y Homeopatía (ENMH), Instituto Politécnico Nacional (IPN), Mexico City, Mexico.
authors.forename: Lea
authors.initials: L
authors.lastname: Alonso-Rangel
authors.affiliation: Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del IPN (Cinvestav-IPN), Mexico City, Mexico.
authors.forename: Bulmaro
authors.initials: B
authors.lastname: Cisneros
authors.affiliation: Department of Biomedical Science, University of Sheffield, Sheffield, United Kingdom.
authors.forename: Steve J
authors.initials: SJ
authors.lastname: Winder
authors.affiliation: Laboratorio de Hematobiología, Escuela Nacional de Medicina y Homeopatía (ENMH), Instituto Politécnico Nacional (IPN), Mexico City, Mexico.
authors.forename: Doris
authors.initials: D
authors.lastname: Cerecedo
mesh_terms: D000199:Actins / Q000378:metabolism*; D002454:Cell Differentiation / Q000502:physiology*; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D015536:Down-Regulation / Q000502:physiology; D049030:Dystroglycans / Q000378:metabolism*; D016189:Dystrophin / Q000378:metabolism; D006801:Humans; D053583:Interleukin-1beta / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000378:metabolism; D009504:Neutrophils / Q000378:metabolism; D034622:RNA Interference / Q000502:physiology
chemical_list: D000199:Actins; D016189:Dystrophin; D053583:Interleukin-1beta; D008562:Membrane Glycoproteins; D049030:Dystroglycans