title: Screening of lignan patterns in Schisandra species using ultrasonic assisted temperature switch ionic liquid microextraction followed by UPLC-MS/MS analysis.
abstract: The ultrasonic assisted temperature-switch ionic liquid microextraction (UATS-ILME) has been successfully applied in extracting of seven lignans from Schisandra. 1-Butyl-3-methylimidazolium tetrafluoroborate ([C4MIM][BF4]) aqueous solution was selected for extracting the target analytes in raw material at 80°C. The lignans were deposited into a single drop by in situ forming 1-butyl-3-methylimidazolium hexafluorophosphate ([C4MIM][PF6]) by cooling down to 0°C and centrifuging for 10min. The extracts were analyzed by ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) in a robust multiple-reaction monitoring (MRM) mode in five minutes. Meanwhile, the proposed method was validated and successfully applied to the determination of seven lignans in twelve Schisandra species. The results indicated that UATS-ILME combined with UPLC-MS/MS is a powerful and practical tool, which has great potential for comprehensive quality control of herbal medicines.
authors.affiliation: Jiangxi Key Laboratory of Mining and Metallurgy Environmental Pollution Control, School of Resource and Environmental Engineering, Jiangxi University of Science and Technology, Ganzhou 341000, Jiangxi, PR China. Electronic address: weidong1006@163.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Dong
authors.affiliation: Jiangxi Key Laboratory of Mining and Metallurgy Environmental Pollution Control, School of Resource and Environmental Engineering, Jiangxi University of Science and Technology, Ganzhou 341000, Jiangxi, PR China.
authors.forename: Shuijing
authors.initials: S
authors.lastname: Yu
authors.affiliation: Jiangxi Key Laboratory of Mining and Metallurgy Environmental Pollution Control, School of Resource and Environmental Engineering, Jiangxi University of Science and Technology, Ganzhou 341000, Jiangxi, PR China.
authors.forename: Yangwu
authors.initials: Y
authors.lastname: Deng
authors.affiliation: Jiangxi Key Laboratory of Mining and Metallurgy Environmental Pollution Control, School of Resource and Environmental Engineering, Jiangxi University of Science and Technology, Ganzhou 341000, Jiangxi, PR China.
authors.forename: Tao
authors.initials: T
authors.lastname: Pan
mesh_terms: D052578:Ionic Liquids / Q000737:chemistry*; D017705:Lignans / Q000032:analysis*; D032141:Schisandra / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013696:Temperature; D014465:Ultrasonics
chemical_list: D052578:Ionic Liquids; D017705:Lignans
keywords: Ionic liquid microextractoin; Liquid chromatography tandem mass spectrometry; Schisandra; Ultrasonic assisted extraction