title: Amentoflavone Induces Anti-angiogenic and Anti-metastatic Effects Through Suppression of NF-κB Activation in MCF-7 cells.
abstract: The aim of the present study was to investigate whether amentoflavone induces anti-angiogenic and anti-metastatic effects through suppression of NF-κB activation in breast cancer in vitro. Effects of NF-κB inhibitor 4-N-[2-(4-phenoxyphenyl) ethyl] quinazoline-4, 6-diamine (QNZ) and amentoflavone on the expression and secretion of angiogenesis- and metastasis-related proteins and cell invasion were investigated by western blotting, enzyme-linked immunosorbent assay (ELISA), and invasion assays. We also verified the effects of QNZ and amentoflavone on lipopolysaccharides (LPS)-activated cell invasion. Obtained results indicated that both QNZ and amentoflavone reduce NF-κB activation, expression and secretion of angiogenesis- and metastasis-related proteins, and cell invasion. QNZ and amentoflavone also reverse LPS-activated cell invasion. In conclusion, inhibition of NF-κB activation decreases expression and secretion of angiogenesis- and metastasis-related proteins. Amentoflavone may induce anti-angiogenic and anti-metastatic effects through suppression of NF-κB activation.
authors.affiliation: Department of Emergency Medicine, Cathay General Hospital, Taipei, Taiwan, R.O.C. School of Medicine, Fu-Jen Catholic University, Taipei, Taiwan, R.O.C. School of Medicine, Taipei Medical University, Taipei, Taiwan, R.O.C.
authors.forename: Jiann-Hwa
authors.initials: JH
authors.lastname: Chen
authors.affiliation: Department of Emergency Medicine, Cathay General Hospital, Taipei, Taiwan, R.O.C. School of Medicine, Fu-Jen Catholic University, Taipei, Taiwan, R.O.C.
authors.forename: Wei-Lung
authors.initials: WL
authors.lastname: Chen
authors.affiliation: Department of Radiation Oncology, National Yang-Ming University Hospital, Yilan, Taiwan, R.O.C. kevinyc.liu@gmail.com.
authors.forename: Yu-Chang
authors.initials: YC
authors.lastname: Liu
mesh_terms: D044946:Biflavonoids / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use*; D045744:Cell Line, Tumor; D065688:Cytochrome P-450 CYP2C9 Inhibitors / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D061986:MCF-7 Cells; D016328:NF-kappa B / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D044946:Biflavonoids; D065688:Cytochrome P-450 CYP2C9 Inhibitors; D016328:NF-kappa B; C011164:amentoflavone
keywords: Amentoflavone; NF-κB; anti-angiogenesis; anti-metastasis