title: Immunohistochemical analysis of Bax and AIF in colorectal tumors.
abstract: OBJECTIVE
  This study had three aims. The first was to determine whether Bax (Bcl-2-associated X protein) and AIF (apoptosis-inducing factor) are expressed in tissue sections of colorectal tumors. The second was to ascertain whether there is any difference in Bax and AIF expression between colorectal polyps, adenomas, and carcinomas. The third aim was to determine whether there is any difference between Bax and AIF expression in colorectal tumors.
  
  
  METHODS
  Bax and AIF expression were determined in 20 hyperplastic polyps (HPs), 20 adenomatous polyps (APs), 20 samples of colorectal carcinomas, and 20 samples of normal mucosa by immunohistochemical methods.
  
  
  RESULTS
  The staining level of Bax and AIF in adenomas and carcinomas was significantly higher than in normal tissues (P<0.01). There was also a significant difference between HPs and APs (P<0.01). The level of Bax and AIF in carcinomas was higher than in adenomas, and the difference was of statistical significance (P<0.01).
  
  
  CONCLUSIONS
  This study may be of interest in future research to confirm whether the changed expression of Bax and/or AIF between benign and malignant tumors can provide valuable information for the evaluation of colon or other tumors.
authors.affiliation: Department of Pathology, Istanbul-Turkıye, Medeniyet University, Goztepe Education and Research Hospital Turkıye.
authors.forename: Bengu
authors.initials: B
authors.lastname: Cobanoglu
authors.affiliation: Department of Pathology, Istanbul-Turkıye, Medeniyet University, Goztepe Education and Research Hospital Turkıye.
authors.forename: A Bahar
authors.initials: AB
authors.lastname: Ceyran
authors.affiliation: Department of Anesthesiology and Reanimation, Siyami Ersek Thoracic and Cardiovascular Surgery Training and Research Hospital Istanbul, Turkıye.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Simsek
authors.affiliation: Department of Pathology, Istanbul-Turkıye, Medeniyet University, Goztepe Education and Research Hospital Turkıye.
authors.forename: Serkan
authors.initials: S
authors.lastname: Şenol
keywords: Bcl-2-associated X protein; Colorectal neoplasms; apoptosis; apoptosis-inducing factor; immunohistochemistry