title: Purification of a thermostable antinociceptive lectin isolated from Andira anthelmia.
abstract: Andira anthelmia (tribe Dalbergieae), a plant from Brazilian Amazon, possesses a seed lectin that was purified by affinity chromatography in sepharose-mannose. This novel Dalbergieae lectin, named AAL, agglutinated rabbit erythrocytes treated with trypsin. The hemagglutinating activity of AAL was maintained after incubation at a wide range of temperature (40 to 70 °C) and pH, was shown to be dependent on divalent cations, and was inhibited by d-mannose and d-sucrose. AAL showed an electrophoretic profile in sodium dodecyl sulfate-polyacrylamide gel electrophoresis similar to other lectins of the tribe Dalbergieae, presenting a double band of molecular weight with approximately 20 kDa and other minor bands of 17, 15, and 13 kDa, being the smaller fragment glycosylated. AAL injected by intravenous route in mice showed antinociceptive activity in two behavioral tests (writhing and formalin). In the writhing test induced by acetic acid, AAL showed inhibitory effect at 0.01 mg/kg (68%), 0.1 mg/kg (46%) and 1 mg/kg (74%). In the formalin test, AAL (0.1 mg/kg) inhibited by 48% the licking time in the inflammatory phase, an effect that was recovered by the lectin association with mannose. In conclusion, AAL presents analgesic effect involving the lectin domain via peripheral mechanisms of inflammatory nociception. This activity highlights the importance of lectins as tools to be used for understanding the interaction of protein-carbohydrate in processes associated to inflammatory pain. Copyright © 2015 John Wiley & Sons, Ltd.
authors.affiliation: Laboratório de Moléculas Biologicamente Ativas (Biomol-Lab), Department of Biochemistry, Molecular Biology, Federal University of Ceará, Av Mister Hull s/n, Bloco 907, Lab. 1075, Campus do Pici, Fortaleza, CE, 60440-970, Brazil.
authors.forename: Kyria Santiago
authors.initials: KS
authors.lastname: Nascimento
authors.affiliation: Laboratório de Fisio-Farmacologia da Inflamação (LAFFIN), Institute of Biomedical Sciences, State University of Ceará, Av. Paranjana 1700, 60740-000, Fortaleza, Ceará, Brazil.
authors.forename: Francisco Lucas Faustino do
authors.initials: FL
authors.lastname: Nascimento
authors.affiliation: Laboratório de Moléculas Biologicamente Ativas (Biomol-Lab), Department of Biochemistry, Molecular Biology, Federal University of Ceará, Av Mister Hull s/n, Bloco 907, Lab. 1075, Campus do Pici, Fortaleza, CE, 60440-970, Brazil.
authors.forename: Mayara Torquato Lima
authors.initials: MT
authors.lastname: Silva
authors.affiliation: Laboratório de Moléculas Biologicamente Ativas (Biomol-Lab), Department of Biochemistry, Molecular Biology, Federal University of Ceará, Av Mister Hull s/n, Bloco 907, Lab. 1075, Campus do Pici, Fortaleza, CE, 60440-970, Brazil.
authors.forename: Camila Bezerra
authors.initials: CB
authors.lastname: Nobre
authors.affiliation: Laboratório de Moléculas Biologicamente Ativas (Biomol-Lab), Department of Biochemistry, Molecular Biology, Federal University of Ceará, Av Mister Hull s/n, Bloco 907, Lab. 1075, Campus do Pici, Fortaleza, CE, 60440-970, Brazil.
authors.forename: Cleane Gomes
authors.initials: CG
authors.lastname: Moreira
authors.affiliation: Laboratório de Moléculas Biologicamente Ativas (Biomol-Lab), Department of Biochemistry, Molecular Biology, Federal University of Ceará, Av Mister Hull s/n, Bloco 907, Lab. 1075, Campus do Pici, Fortaleza, CE, 60440-970, Brazil.
authors.forename: Luiz André Cavalcante
authors.initials: LA
authors.lastname: Brizeno
authors.affiliation: Centro Universitário Christus - Unichristus, Campus Parque Ecológico, Rua João Adolfo Gurgel, 133 - Cocó, Fortaleza, Ceará, 60190-060, Brazil.
authors.forename: Edson Lopes
authors.initials: EL
authors.lastname: da Ponte
authors.affiliation: Laboratório de Fisio-Farmacologia da Inflamação (LAFFIN), Institute of Biomedical Sciences, State University of Ceará, Av. Paranjana 1700, 60740-000, Fortaleza, Ceará, Brazil.
authors.forename: Ana Maria Sampaio
authors.initials: AM
authors.lastname: Assreuy
authors.affiliation: Laboratório de Moléculas Biologicamente Ativas (Biomol-Lab), Department of Biochemistry, Molecular Biology, Federal University of Ceará, Av Mister Hull s/n, Bloco 907, Lab. 1075, Campus do Pici, Fortaleza, CE, 60440-970, Brazil.
authors.forename: Benildo Sousa
authors.initials: BS
authors.lastname: Cavada
mesh_terms: D000700:Analgesics / Q000008:administration & dosage* / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D000818:Animals; D002846:Chromatography, Affinity; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000187:drug effects; D007887:Fabaceae / Q000737:chemistry*; D006386:Hemagglutination Tests; D006863:Hydrogen-Ion Concentration; D008358:Mannose / Q000494:pharmacology; D051379:Mice; D008970:Molecular Weight; D010146:Pain / Q000188:drug therapy* / Q000209:etiology; D037121:Plant Lectins / Q000008:administration & dosage* / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D055550:Protein Stability; D011817:Rabbits; D013395:Sucrose / Q000494:pharmacology; D013696:Temperature
chemical_list: D000700:Analgesics; D037121:Plant Lectins; D013395:Sucrose; D008358:Mannose
keywords: Andira anthelmia; leguminous lectin; nociception; partial characterization