title: Patch tracheoplasty in body tissue engineering using collagenous connective tissue membranes (biosheets).
abstract: BACKGROUND
  Collagenous connective tissue membranes (biosheets) are useful for engineering cardiovascular tissue in tissue engineering. The aim was to evaluate the use of biosheets as a potential tracheal substitute material in vivo in a rabbit model.
  
  
  METHODS
  Group 1: Rectangular-shaped Gore-Tex (4×7mm) was implanted into a 3×6mm defect created in the midventral portion of the cervical trachea. Group 2: Rectangular-shaped dermis was implanted into a tracheotomy of similar size. Group 3: Biosheets were prepared by embedding silicone moulds in dorsal subcutaneous pouches in rabbits for 1month. Rectangular-shaped biosheets were implanted into a tracheotomy of similar size in an autologous fashion. All groups (each containing 10 animals) were sacrificed 4weeks after implantation.
  
  
  RESULTS
  All materials maintained airway structure for up to 4weeks after implantation. Regenerative cartilage in implanted Biosheets in group 3 was confirmed by histological analysis. Tracheal epithelial regeneration occurred in the internal lumen of group 3. There were significant differences in the amounts of collagen type II and glycosaminoglycan between group 3 and group 1 or 2.
  
  
  CONCLUSIONS
  We confirm that cartilage can self-regenerate onto an airway patch using Biosheets.
authors.affiliation: Department of Pediatric Surgery, Saitama Medical University, 38 Morohongo, Moroyama-cho, Iruma-gun, Saitama 350-0495, Japan.
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Satake
authors.affiliation: Department of Pediatric Surgery, Saitama Medical University, 38 Morohongo, Moroyama-cho, Iruma-gun, Saitama 350-0495, Japan; Department of Pediatric Surgery, Tokyo University, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan. Electronic address: komura-tky@umin.ac.jp.
authors.forename: Makoto
authors.initials: M
authors.lastname: Komura
authors.affiliation: Department of Pediatric Surgery, Tokyo University, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan.
authors.forename: Hiroko
authors.initials: H
authors.lastname: Komura
authors.affiliation: Department of Pediatric Surgery, Saitama Medical University, 38 Morohongo, Moroyama-cho, Iruma-gun, Saitama 350-0495, Japan.
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Kodaka
authors.affiliation: Department of Pediatric Surgery, Saitama Medical University, 38 Morohongo, Moroyama-cho, Iruma-gun, Saitama 350-0495, Japan.
authors.forename: Kan
authors.initials: K
authors.lastname: Terawaki
authors.affiliation: Department of Pediatric Surgery, Saitama Medical University, 38 Morohongo, Moroyama-cho, Iruma-gun, Saitama 350-0495, Japan.
authors.forename: Kenichi
authors.initials: K
authors.lastname: Ikebukuro
authors.affiliation: Department of Pediatric Surgery, Saitama Medical University, 38 Morohongo, Moroyama-cho, Iruma-gun, Saitama 350-0495, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Komuro
authors.affiliation: Department of Pediatric Surgery, Saitama Medical University, 38 Morohongo, Moroyama-cho, Iruma-gun, Saitama 350-0495, Japan.
authors.forename: Hironobu
authors.initials: H
authors.lastname: Yonekawa
authors.affiliation: Division of Tissue Engineering, Graduate School of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan.
authors.forename: Kazuto
authors.initials: K
authors.lastname: Hoshi
authors.affiliation: Division of Tissue Engineering, Graduate School of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan.
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Takato
authors.affiliation: Division of Medical Engineering and Materials, National Cerebral and Cardiovascular Centre Research Institute, 5-7-1 Fujishiro-dai, Suita, Osaka 565-8565, Japan.
authors.forename: Yasuhide
authors.initials: Y
authors.lastname: Nakayama
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D002356:Cartilage / Q000502:physiology*; D003238:Connective Tissue / Q000502:physiology*; D005260:Female; D048091:Guided Tissue Regeneration / Q000379:methods*; D011138:Polytetrafluoroethylene; D011817:Rabbits; D012038:Regeneration; D020545:Respiratory Mucosa / Q000502:physiology*; D012828:Silicones; D054457:Tissue Scaffolds; D014132:Trachea / Q000502:physiology / Q000601:surgery*; D014140:Tracheotomy
chemical_list: D001672:Biocompatible Materials; D012828:Silicones; D011138:Polytetrafluoroethylene
keywords: Biosheets; Collagenous connective tissue membranes; Mesenchymal connective tissue; Patch tracheoplasty; Tissue engineering