title: Toxicity of nanosilver in intragastric studies: Biodistribution and metabolic effects.
abstract: The unique physicochemical properties of silver nanoparticles explain their extensive application in consumer goods, food, and medicinal products. However, the biological effects of nanosilver after peroral exposure of mammals are still debatable. This study describes the biodistribution and biological action of 12nm non-coated silver nanoparticles intragastrically administered to male rats after acute (single exposure) and sub-acute (multiple exposures over 30 days) toxicity experiments. The daily doses were 2000 and 250mg/kg of body weight for single and multiple administrations, respectively. Silver tissue detection was conducted by elemental analysis with the help of atomic absorption spectroscopy. An estimation of the state of exposed animals was made and the dynamics of hematological and biochemical parameters of rats was studied. It was demonstrated that single and multiple administrations resulted in silver accumulation in the liver, kidneys, spleen, stomach, and small intestine. After both one- and repeated-dose exposures, the highest Ag contents were detected in the liver (0.87±0.37μg/g of organ) and kidneys (0.24±0.02μg/g of organ). The concentrations of silver detected in tissues were far smaller than the administered doses (<99%), indicating its efficient excretion from the organism. Acute and sub-acute exposures caused no animal mortality or signs of toxicity, manifested as changes in outward appearance or notable deviations in behavior or locomotor activity. Postmortem study revealed no visible pathomorphological abnormalities of internal organs. Hematological indices and biochemical parameters of the treated rats did not differ from those of the vehicle control animals. Overall, it can be concluded that nanosilver is able to be absorbed from the gastrointestinal tract into the bloodstream and accumulate in the secondary organs of rats. It showed no distinct toxicity under the experimental conditions of this study.
authors.affiliation: Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, 33, Bld. 2 Leninsky Ave., Moscow 119071, Russia.
authors.forename: Olga D
authors.initials: OD
authors.lastname: Hendrickson
authors.affiliation: Institute of Physiologically Active Compounds, Russian Academy of Sciences, Severny proezd 1, 142432 Chernogolovka, Moscow Region, Russia.
authors.forename: Sergey G
authors.initials: SG
authors.lastname: Klochkov
authors.affiliation: Institute of Physiologically Active Compounds, Russian Academy of Sciences, Severny proezd 1, 142432 Chernogolovka, Moscow Region, Russia.
authors.forename: Oksana V
authors.initials: OV
authors.lastname: Novikova
authors.affiliation: Institute of Physiologically Active Compounds, Russian Academy of Sciences, Severny proezd 1, 142432 Chernogolovka, Moscow Region, Russia.
authors.forename: Irina M
authors.initials: IM
authors.lastname: Bravova
authors.affiliation: Institute of Physiologically Active Compounds, Russian Academy of Sciences, Severny proezd 1, 142432 Chernogolovka, Moscow Region, Russia.
authors.forename: Elena F
authors.initials: EF
authors.lastname: Shevtsova
authors.affiliation: Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, 33, Bld. 2 Leninsky Ave., Moscow 119071, Russia.
authors.forename: Irina V
authors.initials: IV
authors.lastname: Safenkova
authors.affiliation: Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, 33, Bld. 2 Leninsky Ave., Moscow 119071, Russia.
authors.forename: Anatoly V
authors.initials: AV
authors.lastname: Zherdev
authors.affiliation: Institute of Physiologically Active Compounds, Russian Academy of Sciences, Severny proezd 1, 142432 Chernogolovka, Moscow Region, Russia.
authors.forename: Sergey O
authors.initials: SO
authors.lastname: Bachurin
authors.affiliation: Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, 33, Bld. 2 Leninsky Ave., Moscow 119071, Russia. Electronic address: ohendrick@mail.ru.
authors.forename: Boris B
authors.initials: BB
authors.lastname: Dzantiev
mesh_terms: D000818:Animals; D001772:Blood Cell Count; D001774:Blood Chemical Analysis; D004305:Dose-Response Relationship, Drug; D007441:Intubation, Gastrointestinal; D008297:Male; D053768:Metal Nanoparticles / Q000008:administration & dosage / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley; D018030:Silver Compounds / Q000008:administration & dosage / Q000493:pharmacokinetics* / Q000633:toxicity*; D013054:Spectrophotometry, Atomic; D014018:Tissue Distribution
chemical_list: D018030:Silver Compounds
keywords: Biodistribution; In vivo toxicology; Intragastric administration; Silver nanoparticles