title: Butelase 1: A Versatile Ligase for Peptide and Protein Macrocyclization.
abstract: Macrocyclization is a valuable tool for drug design and protein engineering. Although various methods have been developed to prepare macrocycles, a general and efficient strategy is needed. Here we report a highly efficient method using butelase 1 to macrocyclize peptides and proteins ranging in sizes from 26 to >200 residues. We achieved cyclizations that are 20,000 times faster than sortase A, the most widely used ligase for protein cyclization. The reactions completed within minutes with up to 95% yields.
authors.affiliation: School of Biological Sciences, Nanyang Technological University , 60 Nanyang Drive, Singapore.
authors.forename: Giang K T
authors.initials: GK
authors.lastname: Nguyen
authors.affiliation: School of Biological Sciences, Nanyang Technological University , 60 Nanyang Drive, Singapore.
authors.forename: Antony
authors.initials: A
authors.lastname: Kam
authors.affiliation: School of Biological Sciences, Nanyang Technological University , 60 Nanyang Drive, Singapore.
authors.forename: Shining
authors.initials: S
authors.lastname: Loo
authors.affiliation: School of Biological Sciences, Nanyang Technological University , 60 Nanyang Drive, Singapore.
authors.forename: Anna E
authors.initials: AE
authors.lastname: Jansson
authors.affiliation: School of Biological Sciences, Nanyang Technological University , 60 Nanyang Drive, Singapore.
authors.forename: Lucy X
authors.initials: LX
authors.lastname: Pan
authors.affiliation: School of Biological Sciences, Nanyang Technological University , 60 Nanyang Drive, Singapore.
authors.forename: James P
authors.initials: JP
authors.lastname: Tam
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003500:Cyclization; D006801:Humans; D008025:Ligases / Q000737:chemistry / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010453:Peptide Synthases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D051381:Rats
chemical_list: D010455:Peptides; D010940:Plant Proteins; D011506:Proteins; D008025:Ligases; D010453:Peptide Synthases