title: Camphanic acid chloride: a powerful derivatization reagent for stereoisomeric separation and its DMPK applications.
abstract: BACKGROUND
  Camphanic acid chloride has proven to be an efficient chiral derivatization reagent for determination of stereoisomers.
  
  
  RESULTS
  The utility of chemical derivatization of various stereoisomers containing hydroxy functional groups with camphanic acid chloride in the presence or absence of a base is highlighted. This procedure is shown to be relatively simple, fast and a cost-effective method of separating racemic drugs and stereoisomeric metabolites in biological matrices. Camphanic derivatives contain two additional chirogenic centers, which are found to enhance stereoisomeric separation on both traditional and chiral stationary phases.
  
  
  CONCLUSIONS
  Four methodologies described herein for separation of multiple stereoisomers in biological samples confirm camphanic acid chloride to be a powerful chiral reagent for stereoisomeric resolution for drug metabolism and PK applications.
authors.affiliation: Bioanalytical Science & Toxicokinetics, Platform Science & Technology, GlaxoSmithkline Pharmaceuticals, 709 Swedeland Road, King of Prussia, PA 19406, USA.
authors.forename: Hermes
authors.initials: H
authors.lastname: Licea-Perez
authors.affiliation: Bioanalytical Science & Toxicokinetics, Platform Science & Technology, GlaxoSmithkline Pharmaceuticals, 709 Swedeland Road, King of Prussia, PA 19406, USA.
authors.forename: Sherry
authors.initials: S
authors.lastname: Wang
authors.affiliation: Bioanalytical Science & Toxicokinetics, Platform Science & Technology, GlaxoSmithkline Pharmaceuticals, 709 Swedeland Road, King of Prussia, PA 19406, USA.
authors.forename: Ciara
authors.initials: C
authors.lastname: Rodgers
authors.affiliation: Bioanalytical Science & Toxicokinetics, Platform Science & Technology, GlaxoSmithkline Pharmaceuticals, 709 Swedeland Road, King of Prussia, PA 19406, USA.
authors.forename: Chester L
authors.initials: CL
authors.lastname: Bowen
authors.affiliation: Bioanalytical Science & Toxicokinetics, Platform Science & Technology, GlaxoSmithkline Pharmaceuticals, 709 Swedeland Road, King of Prussia, PA 19406, USA.
authors.forename: Kasie
authors.initials: K
authors.lastname: Fang
authors.affiliation: Bioanalytical Science & Toxicokinetics, Platform Science & Technology, GlaxoSmithkline Pharmaceuticals, 709 Swedeland Road, King of Prussia, PA 19406, USA.
authors.forename: Matthew
authors.initials: M
authors.lastname: Szapacs
authors.affiliation: Bioanalytical Science & Toxicokinetics, Platform Science & Technology, GlaxoSmithkline Pharmaceuticals, 709 Swedeland Road, King of Prussia, PA 19406, USA.
authors.forename: Christopher A
authors.initials: CA
authors.lastname: Evans
mesh_terms: D001952:Bridged-Ring Compounds / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D007783:Lactones / Q000737:chemistry*; D065636:Myotonin-Protein Kinase / Q000737:chemistry*; D013237:Stereoisomerism
chemical_list: D001952:Bridged-Ring Compounds; C585517:DMPK protein, human; D007783:Lactones; C500772:camphanic acid; D065636:Myotonin-Protein Kinase
keywords: UPC2; camphanic acid chloride; chiral derivatization; chiral separation; convergence chromatography; stereoisomers; supercritical fluid chromatography (SFC)