title: Detection of AmpC β-lactamases inAcinetobacter baumanniiin the Xuzhou region and analysis of drug resistance.
abstract: The aim of the present study was to determine the prevalence and related drug resistance of AmpC β-lactamases inAcinetobacter baumanniiin tertiary-level hospitals in the Xuzhou region in China. A total of 134 clinical isolates of non-repetitiveAcinetobacter baumanniiwere collected from different hospitals in the Xuzhou region, and multiplex polymerase chain reaction (PCR) was employed to determine the genotype of AmpC. The PCR products were purified and sequenced. The susceptibility to antibiotics was tested using the biometrics automated microbiological-assay system, VITEK-2. Amongst the 134 isolated strains, 96 strains were found to produce AmpC β-lactamases, and the positive rate was 72%, all of which carried acinetobacter-derived cephalosporinase (ADC) type AmpC resistance genes. The drug sensitivity tests indicated that the positiveAcinetobacter baumanniistrains were resistant to the majority of extended-spectrum β-lactam antibiotics, but were only sensitive to polymyxin. In conclusion, the incidence of AmpC enzymes inAcinetobacter baumanniistrains in tertiary-level hospitals in the Xuzhou area is relatively high, and resistance to the majority of extended-spectrum β-lactam antibiotics may be related to the ADC type of AmpC.
authors.affiliation: Department of Respiration, The First People's Hospital of Jining, Shandong 272002, P.R. China.
authors.forename: Yongrui
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Respiration, Xuzhou City Hospital Affiliated to Xuzhou Medical College, Xuzhou, Jiangsu 221002, P.R. China.
authors.forename: Xiangqun
authors.initials: X
authors.lastname: Liu
keywords: Acinetobacter baumannii; AmpC β-lactamases; acinetobacter-derived cephalosporinase; polymerase chain reaction