title: Comparative evaluation of Nabi and Beltsville extenders for cryopreservation of rooster semen.
abstract: Two experiments were conducted to evaluate the new rooster semen freezing extender which is containing a low level of glycerol and soybean lecithin as an alternative protective agent in the extender. The aim of the first experiment was to evaluate a new extender for freeze-thawing rooster semen known as "Nabi" extender compared to Beltsville. Second experiment was also performed to determine whether the Nabi extender has negative reactions on fertilization after artificial insemination (AI) or no. In the first experiment, post-thaw motion parameters, mitochondrial function and sperm apoptosis were analyzed using Sperm Class Analyzer (SCA), rhodamine-123 and Annexin-V, respectively for frozen-thawed semen in Nabi and Beltsville extender. Results showed that total motility, progressive motility, velocity parameters (VCL, VSL, VAP, LIN and STR) and live spermatozoa with active mitochondria were significantly higher in Nabi compare to Beltsville extender (P < 0.01). Also, the percentages of post-thawed live and early apoptotic spermatozoa were significantly higher in Nabi compared to Beltsville extender (14.46 ± 0.95 vs. 19.27 ± 0.95 and 14.83 ± 4.51 vs. 39.27 ± 4.51, respectively). For apoptotic spermatozoa, the percentages of post-thawed late apoptotic spermatozoa were significantly lower in Nabi (29.66 ± 3.11) compared to Beltsville extender (69.07 ± 3.11), but the type of extender had no effect on the percentages of post-thawed necrotic spermatozoa. In the second experiment, 20 broiler breeder hens (Ross 308) were inseminated with thawed semen using the new freezing diluents or fresh semen for determination of fertility rate. Fertility rate with thawed semen (with Nabi extender) was lower compared to fresh semen (by approximately 8% points). It can be concluded that Nabi extender would improve post-thawed rooster sperm in vitro quality compared to Beltsville extender. The fertility rates of insemination in hens with freeze-thaw sperm were comparable with fresh sperm.
authors.affiliation: Department of Animal Science, Faculty College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran. Electronic address: Medi_nabi@ut.ac.ir.
authors.forename: Mohammad Mahdi
authors.initials: MM
authors.lastname: Nabi
authors.affiliation: Department of Animal Science, Faculty College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran; Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University, Ahvaz, Iran.
authors.forename: Hamid
authors.initials: H
authors.lastname: Kohram
authors.affiliation: Department of Animal Science, Faculty College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.
authors.forename: Mahdi
authors.initials: M
authors.lastname: Zhandi
authors.affiliation: Department of Animal Science, Faculty College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.
authors.forename: Hassan
authors.initials: H
authors.lastname: Mehrabani-Yeganeh
authors.affiliation: Department of Animal Science, Faculty College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.
authors.forename: Hossein
authors.initials: H
authors.lastname: Sharideh
authors.affiliation: Department of Animal Science, Faculty College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.
authors.forename: Ahmad
authors.initials: A
authors.lastname: Zare-Shahaneh
authors.affiliation: Department of Embryology at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.
authors.forename: Vahid
authors.initials: V
authors.lastname: Esmaili
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002645:Chickens; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology*; D005260:Female; D005298:Fertility / Q000187:drug effects; D005990:Glycerol / Q000494:pharmacology; D007315:Insemination, Artificial / Q000379:methods; D008297:Male; D008928:Mitochondria / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology; D012661:Semen / Q000378:metabolism; D055101:Semen Analysis; D012662:Semen Preservation / Q000379:methods*; D013025:Glycine max / Q000378:metabolism; D013094:Spermatozoa / Q000378:metabolism*
chemical_list: D003451:Cryoprotective Agents; D010936:Plant Extracts; D005990:Glycerol
keywords: Beltsville; Flow cytometry; Freezing; Rooster; Sperm