title: Interferon response of the cystic fibrosis bronchial epithelium to major and minor group rhinovirus infection.
abstract: Rhinoviruses (RVs) are associated with exacerbations of cystic fibrosis (CF), asthma and COPD. There is growing evidence suggesting the involvement of the interferon (IFN) pathway in RV-associated morbidity in asthma and COPD. The mechanisms of RV-triggered exacerbations in CF are poorly understood. In a pilot study, we assessed the antiviral response of CF and healthy bronchial epithelial cells (BECs) to RV infection, we measured the levels of IFNs, pattern recognition receptors (PRRs) and IFN-stimulated genes (ISGs) upon infection with major and minor group RVs and poly(IC) stimulation. Major group RV infection of CF BECs resulted in a trend towards a diminished IFN response at the level of IFNs, PRRs and ISGs in comparison to healthy BECs. Contrary to major group RV, the IFN pathway induction upon minor group RV infection was significantly increased at the level of IFNs and PRRs in CF BECs compared to healthy BECs.
authors.affiliation: Division of Paediatric Respiratory Medicine, University Children's Hospital, Bern, Switzerland; Department of Clinical Research, University of Bern, Bern, Switzerland; Graduate School for Cellular and Biomedical Sciences, University of Bern, Bern, Switzerland.
authors.forename: Aline
authors.initials: A
authors.lastname: Schögler
authors.affiliation: Division of Paediatric Respiratory Medicine, University Children's Hospital, Bern, Switzerland; Department of Clinical Research, University of Bern, Bern, Switzerland.
authors.forename: Andrea B
authors.initials: AB
authors.lastname: Stokes
authors.affiliation: Division of Paediatric Respiratory Medicine, University Children's Hospital, Bern, Switzerland.
authors.forename: Carmen
authors.initials: C
authors.lastname: Casaulta
authors.affiliation: Division of Paediatric Respiratory Medicine, Lucerne Children's Hospital, Lucerne, Switzerland.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Regamey
authors.affiliation: Airway Disease Infection Section, MRC and Asthma UK Centre in Allergic Mechanisms of Asthma, National Heart and Lung Institute, Imperial College London, London, United Kingdom.
authors.forename: Michael R
authors.initials: MR
authors.lastname: Edwards
authors.affiliation: Airway Disease Infection Section, MRC and Asthma UK Centre in Allergic Mechanisms of Asthma, National Heart and Lung Institute, Imperial College London, London, United Kingdom.
authors.forename: Sebastian L
authors.initials: SL
authors.lastname: Johnston
authors.affiliation: Division of Respiratory Medicine, University Children's Hospital, Zürich, Switzerland.
authors.forename: Andreas
authors.initials: A
authors.lastname: Jung
authors.affiliation: Division of Respiratory Medicine, University Children's Hospital, Zürich, Switzerland.
authors.forename: Alexander
authors.initials: A
authors.lastname: Moeller
authors.affiliation: Department of Clinical Research, University of Bern, Bern, Switzerland; Department of Pulmonary Medicine, University Hospital of Bern, Bern, Switzerland.
authors.forename: Thomas
authors.initials: T
authors.lastname: Geiser
authors.affiliation: Division of Paediatric Respiratory Medicine, University Children's Hospital, Bern, Switzerland; Department of Clinical Research, University of Bern, Bern, Switzerland. Electronic address: marco.alves@dkf.unibe.ch.
authors.forename: Marco P
authors.initials: MP
authors.lastname: Alves
mesh_terms: D018929:Cell Culture Techniques; D003550:Cystic Fibrosis / Q000150:complications / Q000276:immunology / Q000821:virology; D006801:Humans; D018928:Immunity, Mucosal; D007369:Interferon Inducers / Q000276:immunology; D007372:Interferons / Q000032:analysis*; D010850:Picornaviridae Infections / Q000150:complications / Q000276:immunology; D020545:Respiratory Mucosa / Q000276:immunology*; D012229:Rhinovirus / Q000276:immunology / Q000302:isolation & purification
chemical_list: D007369:Interferon Inducers; D007372:Interferons
keywords: Bronchial epithelial cells; Cystic fibrosis; Interferon; Rhinovirus