title: Understanding of polyhydroxybutyrate production under carbon and phosphorus-limited growth conditions in non-axenic continuous culture.
abstract: In a waste into resource strategy, a selection of polyhydroxybutyrate (PHB)-accumulating organisms from activated sludge was achieved in an open continuous culture under acetic acid and phosphorus limitation. Once the microbial population was selected at a dilution rate (D), an increase in phosphorus limitation degree was applied in order to study the intracellular phosphorus plasticity of selected bacteria and the resulting capacity to produce PHB. Whatever D, all selected populations were able to produce PHB. At a D, the phosphorus availability determined the phosphorus-cell content which in turn fixed the amount of cell. All the remaining carbon was thus directed toward PHB. By decreasing D, microorganisms adapted more easily to higher phosphorus limitation leading to higher PHB content. A one-stage continuous reactor operated at D=0.023h(-)(1) gave reliable high PHB productivity with PHB content up to 80%. A two-stage reactor could ensure better productivity while allowing tuning product quality.
authors.affiliation: Université de Toulouse, INSA, UPS, LISBP, 135 Avenue de Rangueil, F-31077 Toulouse Cedex 4, France; INRA, UMR792 Ingénierie des Systèmes Biologiques et des Procédés, F-31400 Toulouse, France; CNRS, UMR5504, F-31400 Toulouse, France; VEOLIA Environnement, Centre de Recherche sur l'Eau, Chemin de la Digue, BP 76, 78603 Maisons-Laffite Cedex, France.
authors.forename: Laëtitia
authors.initials: L
authors.lastname: Cavaillé
authors.affiliation: VEOLIA Environnement, Centre de Recherche sur l'Eau, Chemin de la Digue, BP 76, 78603 Maisons-Laffite Cedex, France.
authors.forename: Maria
authors.initials: M
authors.lastname: Albuquerque
authors.affiliation: Université de Toulouse, INSA, UPS, LISBP, 135 Avenue de Rangueil, F-31077 Toulouse Cedex 4, France; INRA, UMR792 Ingénierie des Systèmes Biologiques et des Procédés, F-31400 Toulouse, France; CNRS, UMR5504, F-31400 Toulouse, France.
authors.forename: Estelle
authors.initials: E
authors.lastname: Grousseau
authors.affiliation: VEOLIA Environnement, Centre de Recherche sur l'Eau, Chemin de la Digue, BP 76, 78603 Maisons-Laffite Cedex, France.
authors.forename: Anne-Sophie
authors.initials: AS
authors.lastname: Lepeuple
authors.affiliation: Université de Toulouse, INSA, UPS, LISBP, 135 Avenue de Rangueil, F-31077 Toulouse Cedex 4, France; INRA, UMR792 Ingénierie des Systèmes Biologiques et des Procédés, F-31400 Toulouse, France; CNRS, UMR5504, F-31400 Toulouse, France.
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Uribelarrea
authors.affiliation: Université de Toulouse, INSA, UPS, LISBP, 135 Avenue de Rangueil, F-31077 Toulouse Cedex 4, France; INRA, UMR792 Ingénierie des Systèmes Biologiques et des Procédés, F-31400 Toulouse, France; CNRS, UMR5504, F-31400 Toulouse, France.
authors.forename: Guillermina
authors.initials: G
authors.lastname: Hernandez-Raquet
authors.affiliation: Université de Toulouse, INSA, UPS, LISBP, 135 Avenue de Rangueil, F-31077 Toulouse Cedex 4, France; INRA, UMR792 Ingénierie des Systèmes Biologiques et des Procédés, F-31400 Toulouse, France; CNRS, UMR5504, F-31400 Toulouse, France. Electronic address: etienne.paul@insa-toulouse.fr.
authors.forename: Etienne
authors.initials: E
authors.lastname: Paul
mesh_terms: D001419:Bacteria / Q000254:growth & development* / Q000378:metabolism; D002244:Carbon / Q000172:deficiency*; D059013:Microbial Consortia; D010758:Phosphorus / Q000378:metabolism*; D011091:Polyesters / Q000378:metabolism*; D054813:Polyhydroxyalkanoates / Q000096:biosynthesis*; D012722:Sewage; D000094122:Polyhydroxybutyrates
chemical_list: D002244:Carbon; D010758:Phosphorus; D011091:Polyesters; D054813:Polyhydroxyalkanoates; D012722:Sewage; C052620:poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate); D000094122:Polyhydroxybutyrates
keywords: DNL; Mixed population; Open continuous culture; PHB production; Phosphorus