title: The diversity and expansion of the trans-sialidase gene family is a common feature in Trypanosoma cruzi clade members.
abstract: Trans-sialidase (TS) is a polymorphic protein superfamily described in members of the protozoan genus Trypanosoma. Of the eight TS groups recently described, TS group I proteins (some of which have catalytic activity) are present in the distantly related Trypanosoma brucei and Trypanosoma cruzi phylogenetic clades, whereas other TS groups have only been described in some species belonging to the T. cruzi clade. In the present study we analyzed the repertoire, distribution and phylogenetic relationships of TS genes among species of the T. cruzi clade based on sequence similarity, multiple sequence alignment and tree-reconstruction approaches using TS sequences obtained with the aid of PCR-based strategies or retrieved from genome databases. We included the following representative isolates of the T. cruzi clade from South America: T. cruzi, T. cruzi Tcbat, Trypanosoma cruzi marinkellei, Trypanosoma dionisii, Trypanosoma rangeli and Trypanosoma conorhini. The cloned sequences encoded conserved TS protein motifs Asp-box and VTVxNVxLYNR but lacked the FRIP motif (conserved in TS group I). The T. conorhini sequences were the most divergent. The hybridization patterns of TS probes with chromosomal bands confirmed the abundance of these sequences in species in the T. cruzi clade. Divergence and relationship analysis placed most of the TS sequences in the groups defined in T. cruzi. Further examination of members of TS group II, which includes T. cruzi surface glycoproteins implicated in host cell attachment and invasion, showed that sequences of T. cruzi Tcbat grouped with those of T. cruzi genotype TcI. Our analysis indicates that different members of the T. cruzi clade, with different vertebrate hosts, vectors and pathogenicity, share the extensive expansion and sequence diversification of the TS gene family. Altogether, our results are congruent with the evolutionary history of the T. cruzi clade and represent a contribution to the understanding of the molecular evolution and role of TS proteins in trypanosomes.
authors.affiliation: Laboratorio de Genética Molecular "Dr. Yunis-Turbay", Decanato de Ciencias de la Salud, Universidad Centroccidental Lisandro Alvarado, Barquisimeto 3001, Estado Lara, Venezuela. Electronic address: mchiurillo@ucla.edu.ve.
authors.forename: Miguel Angel
authors.initials: MA
authors.lastname: Chiurillo
authors.affiliation: Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP 04023-062, Brazil.
authors.forename: Danielle R
authors.initials: DR
authors.lastname: Cortez
authors.affiliation: Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP 04023-062, Brazil.
authors.forename: Fábio M
authors.initials: FM
authors.lastname: Lima
authors.affiliation: Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP 04023-062, Brazil.
authors.forename: Caroline
authors.initials: C
authors.lastname: Cortez
authors.affiliation: Centro de Biotecnología, Fundación Instituto de Estudios Avanzados, Caracas, Venezuela.
authors.forename: José Luis
authors.initials: JL
authors.lastname: Ramírez
authors.affiliation: Departamento de Parasitologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, SP 05508-900, Brazil.
authors.forename: Andre G
authors.initials: AG
authors.lastname: Martins
authors.affiliation: Department of Microbiology and Immunology, Virginia Commonwealth University, Richmond, VA, USA.
authors.forename: Myrna G
authors.initials: MG
authors.lastname: Serrano
authors.affiliation: Departamento de Parasitologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, SP 05508-900, Brazil.
authors.forename: Marta M G
authors.initials: MM
authors.lastname: Teixeira
authors.affiliation: Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP 04023-062, Brazil.
authors.forename: José Franco
authors.initials: JF
authors.lastname: da Silveira
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D019143:Evolution, Molecular; D006023:Glycoproteins / Q000235:genetics* / Q000378:metabolism; D005810:Multigene Family; D009439:Neuraminidase / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D014349:Trypanosoma cruzi / Q000145:classification / Q000201:enzymology* / Q000235:genetics
chemical_list: D006023:Glycoproteins; C069715:trans-sialidase; D009439:Neuraminidase
keywords: Conserved motif; DTUs; Gene superfamily; Phylogeny; Trans-sialidase; Trypanosoma cruzi clade