title: Role of transcriptional factor Nrf2 in the acute lung injury of mice.
abstract: OBJECTIVE
  This study aimed to investigate the expression and role of Nrf2 in the acute lung injury (ALI) of mice.
  
  
  METHODS
  A total of 60 BABL/c mice were randomly divided into 2 groups: ALI group and control group. In ALI group, ALI was introduced by injection of LPS. Immunohistochemistry was performed to detect Nrf2 expression in the lung; Western blot assay was employed to detect the expression of Nrf2 in the lung homogenate; ELISA was conducted to detect the expression of Nrf2 in the lung homogenate and BALF.
  
  
  RESULTS
  As compared to control group, ALI mice had a high Nrf2 expression in the lung as shown in immunohistochemistry, and the Nrf2 expression in the lung homogenate and BALF also increased markedly (P<0.05).
  
  
  CONCLUSIONS
  The Nrf2 expression increases in the lung and BALF of ALI mice, suggesting that Nrf2 is involved in the inflammation during ALI and may serve as a new target in the therapy of ALI.
authors.affiliation: Department of Emergency, Shanghai Tenth People's Hospital, Tongji University School of Medicine Shanghai 20072, China.
authors.forename: Minghao
authors.initials: M
authors.lastname: Kong
authors.affiliation: Department of Emergency, Shanghai Tenth People's Hospital, Tongji University School of Medicine Shanghai 20072, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Mao
authors.affiliation: Department of Emergency, Shanghai Tenth People's Hospital, Tongji University School of Medicine Shanghai 20072, China.
authors.forename: Bijun
authors.initials: B
authors.lastname: Luo
authors.affiliation: Department of Emergency, Shanghai Tenth People's Hospital, Tongji University School of Medicine Shanghai 20072, China.
authors.forename: Zonghao
authors.initials: Z
authors.lastname: Qin
mesh_terms: D055371:Acute Lung Injury / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D015153:Blotting, Western; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D007150:Immunohistochemistry; D008070:Lipopolysaccharides / Q000633:toxicity; D051379:Mice; D008807:Mice, Inbred BALB C; D051267:NF-E2-Related Factor 2 / Q000378:metabolism*
chemical_list: D008070:Lipopolysaccharides; D051267:NF-E2-Related Factor 2; C495636:Nfe2l2 protein, mouse
keywords: Nrf2; Transcription factor; acute lung injury; mouse