title: Exogenous Expression of Human Protamine 1 (hPrm1) Remodels Fibroblast Nuclei into Spermatid-like Structures.
abstract: Protamines confer a compact structure to the genome of male gametes. Here, we find that somatic cells can be remodeled by transient expression of protamine 1 (Prm1). Ectopically expressed Prm1 forms scattered foci in the nuclei of fibroblasts, which coalescence into spermatid-like structures, concomitant with a loss of histones and a reprogramming barrier, H3 lysine 9 methylation. Protaminized nuclei injected into enucleated oocytes efficiently underwent protamine to maternal histone TH2B exchange and developed into normal blastocyst stage embryos in vitro. Altogether, our findings present a model to study male-specific chromatin remodeling, which can be exploited for the improvement of somatic cell nuclear transfer.
authors.affiliation: Faculty of Veterinary Medicine, University of Teramo, Renato Balzarini Street 1, Campus Coste Sant'Agostino, 64100 Teramo, Italy.
authors.forename: Domenico
authors.initials: D
authors.lastname: Iuso
authors.affiliation: Faculty of Veterinary Medicine, University of Teramo, Renato Balzarini Street 1, Campus Coste Sant'Agostino, 64100 Teramo, Italy.
authors.forename: Marta
authors.initials: M
authors.lastname: Czernik
authors.affiliation: Faculty of Veterinary Medicine, University of Teramo, Renato Balzarini Street 1, Campus Coste Sant'Agostino, 64100 Teramo, Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Toschi
authors.affiliation: Faculty of Veterinary Medicine, University of Teramo, Renato Balzarini Street 1, Campus Coste Sant'Agostino, 64100 Teramo, Italy.
authors.forename: Antonella
authors.initials: A
authors.lastname: Fidanza
authors.affiliation: Institute of Genetics and Animal Breeding of the Polish Academy of Sciences, Postepu 36A, Jastrzębiec, 05-552 Magdalenka, Poland.
authors.forename: Federica
authors.initials: F
authors.lastname: Zacchini
authors.affiliation: Institute of Molecular Genetics (IGMM), CNRS UMR-5535 and University of Montpellier, 1919 route de Mende, 34293 Montpellier, France.
authors.forename: Robert
authors.initials: R
authors.lastname: Feil
authors.affiliation: INSERM, U823, Institut Albert Bonniot, Université Grenoble Alpes, 38700 Grenoble, France.
authors.forename: Sandrine
authors.initials: S
authors.lastname: Curtet
authors.affiliation: INSERM, U823, Institut Albert Bonniot, Université Grenoble Alpes, 38700 Grenoble, France.
authors.forename: Thierry
authors.initials: T
authors.lastname: Buchou
authors.affiliation: INSERM, U823, Institut Albert Bonniot, Université Grenoble Alpes, 38700 Grenoble, France.
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Shiota
authors.affiliation: INSERM, U823, Institut Albert Bonniot, Université Grenoble Alpes, 38700 Grenoble, France.
authors.forename: Saadi
authors.initials: S
authors.lastname: Khochbin
authors.affiliation: Faculty of Veterinary Medicine, University of Teramo, Renato Balzarini Street 1, Campus Coste Sant'Agostino, 64100 Teramo, Italy; Institute of Genetics and Animal Breeding of the Polish Academy of Sciences, Postepu 36A, Jastrzębiec, 05-552 Magdalenka, Poland; National Research Institute of Animal Production 1, Krakowska Street, 32-083 Balice n/Krakow, Poland.
authors.forename: Grazyna Ewa
authors.initials: GE
authors.lastname: Ptak
authors.affiliation: Faculty of Veterinary Medicine, University of Teramo, Renato Balzarini Street 1, Campus Coste Sant'Agostino, 64100 Teramo, Italy. Electronic address: ploi@unite.it.
authors.forename: Pasqualino
authors.initials: P
authors.lastname: Loi
mesh_terms: D000107:Acetylation; D000818:Animals; D002467:Cell Nucleus / Q000737:chemistry / Q000378:metabolism*; D002478:Cells, Cultured; D002843:Chromatin / Q000378:metabolism; D042002:Chromatin Assembly and Disassembly; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D006657:Histones / Q000378:metabolism; D006801:Humans; D008297:Male; D008745:Methylation; D046529:Microscopy, Electron, Transmission; D009865:Oocytes / Q000378:metabolism; D011479:Protamines / Q000235:genetics / Q000378:metabolism*; D012756:Sheep; D013087:Spermatids / Q000737:chemistry / Q000378:metabolism
chemical_list: D002843:Chromatin; D006657:Histones; C082073:PRM1 protein, human; D011479:Protamines; D004247:DNA