title: Identification and characterization of epitopes on Plasmodium knowlesi merozoite surface protein-142 (MSP-142) using synthetic peptide library and phage display library.
abstract: Plasmodium knowlesi can cause potentially life threatening human malaria. The Plasmodium merozoite surface protein-142 (MSP-142) is a potential target for malaria blood stage vaccine, and for diagnosis of malaria. Two epitope mapping techniques were used to identify the potential epitopes within P. knowlesi MSP-142. Nine and 14 potential epitopes were identified using overlapping synthetic peptide library and phage display library, respectively. Two regions on P. knowlesi MSP-142 (amino acid residues 37-95 and residues 240-289) were identified to be the potential dominant epitope regions. Two of the prominent epitopes, P10 (TAKDGMEYYNKMGELYKQ) and P31 (RCLLGFKEVGGKCVPASI), were evaluated using mouse model. P10- and P31-immunized mouse sera reacted with recombinant P. knowlesi MSP-142, with the IgG isotype distribution of IgG2b>IgG1>IgG2a>IgG3. Significant higher level of cytokines interferon-gamma and interleukin-2 was detected in P31-immunized mice. Both P10 and P31 could be the suitable epitope candidates to be used in malaria vaccine designs and immunodiagnostic assays, provided further evaluation is needed to validate the potential uses of these epitopes.
authors.affiliation: Department of Parasitology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Fei Wen
authors.initials: FW
authors.lastname: Cheong
authors.affiliation: Department of Parasitology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia; Tropical Disease Research and Education Centre (TIDREC), Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia. Electronic address: fongmy@um.edu.my.
authors.forename: Mun Yik
authors.initials: MY
authors.lastname: Fong
authors.affiliation: Department of Parasitology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia; Tropical Disease Research and Education Centre (TIDREC), Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Yee Ling
authors.initials: YL
authors.lastname: Lau
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000187:drug effects* / Q000276:immunology; D000953:Antigens, Protozoan / Q000276:immunology*; D001435:Bacteriophages; D016207:Cytokines / Q000187:drug effects* / Q000276:immunology; D018604:Epitope Mapping; D000939:Epitopes / Q000276:immunology*; D007371:Interferon-gamma / Q000187:drug effects / Q000276:immunology; D007376:Interleukin-2 / Q000276:immunology; D008288:Malaria / Q000517:prevention & control*; D017780:Malaria Vaccines / Q000276:immunology / Q000494:pharmacology*; D020066:Merozoite Surface Protein 1 / Q000276:immunology*; D051379:Mice; D019151:Peptide Library; D016790:Plasmodium knowlesi / Q000276:immunology*
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D016207:Cytokines; D000939:Epitopes; D007376:Interleukin-2; D017780:Malaria Vaccines; D020066:Merozoite Surface Protein 1; D019151:Peptide Library; D007371:Interferon-gamma
keywords: Epitope mapping; Phage display library; Plasmodium knowlesi; Synthetic peptide library