title: MiR-223 inhibited cell metastasis of human cervical cancer by modulating epithelial-mesenchymal transition.
abstract: Accumulating evidence have emerged important roles for microRNAs (miRNAs) participating in epithelial-mesenchymal transition (EMT) process and are associated with metastasis in cervical cancer. We hypothesized that miR-223 played an important role in cell metastasis of cervical cancer. Here, we found miR-223 was downregulated in human cervical cancer cell lines and clinical tumor tissues. Result of wound healing and cell migration assays revealed that miR-223 inhibited cell migration, whereas miR-223-in showed the opposite effect. In terms of mechanism, miR-223 influenced the expression of the EMT-associated proteins by upregulating the epithelial markers E-cadherin and α-cadherin and downregulating the mesenchymal marker vimentin. In conclusion, miR-223 inhibited cell metastasis of human cervical cancer by modulating epithelial-mesenchymal transition.
authors.affiliation: Department of Obstetrics and Gynecology, Zhujiang Hospital, Southern Medical University Guangzhou 510282, People's Republic of China ; Department of Obstetrics and Gynecology, The First Affiliated Hospital of Xiamen University Xiamen 361003, People's Republic of China.
authors.forename: Yaling
authors.initials: Y
authors.lastname: Tang
authors.affiliation: Department of Obstetrics and Gynecology, Zhujiang Hospital, Southern Medical University Guangzhou 510282, People's Republic of China.
authors.forename: Yifeng
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Obstetrics and Gynecology, The First Affiliated Hospital of Xiamen University Xiamen 361003, People's Republic of China.
authors.forename: Qionghua
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Department of Obstetrics and Gynecology, The First Affiliated Hospital of Xiamen University Xiamen 361003, People's Republic of China.
authors.forename: Naxuan
authors.initials: N
authors.lastname: Qiu
authors.affiliation: Central Laboratory, The First Affiliated Hospital of Xiamen University Xiamen 361003, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Department of Pathology, The First Affiliated Hospital of Xiamen University Xiamen 361003, People's Republic of China.
authors.forename: Xueye
authors.initials: X
authors.lastname: You
mesh_terms: D015703:Antigens, CD; D015820:Cadherins / Q000235:genetics / Q000378:metabolism; D054443:Cell Migration Assays; D002465:Cell Movement; D015536:Down-Regulation; D058750:Epithelial-Mesenchymal Transition; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006367:HeLa Cells; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D015398:Signal Transduction; D014162:Transfection; D002583:Uterine Cervical Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D014746:Vimentin / Q000235:genetics / Q000378:metabolism
chemical_list: D015703:Antigens, CD; D015820:Cadherins; D035683:MicroRNAs; D014746:Vimentin; C513384:CDH1 protein, human; C525067:MIR223, human
keywords: cell metastasis; human cervical cancer; miR-223