title: Phylogenetic Analysis of Hemagglutinin Genes of H9N2 Avian Influenza Viruses Isolated from Chickens in Shandong, China, between 1998 and 2013.
abstract: Since H9N2 avian influenza virus (AIV) was first isolated in Guangdong province of China, the virus has been circulating in chicken flocks in mainland China. However, a systematic phylogenetic analysis of H9N2 AIV from chickens in Shandong of China has not been conducted. Based on hemagglutinin (HA) gene sequences of H9N2 AIVs isolated from chickens in Shandong of China between 1998 and 2013, genetic evolution of 35 HA gene sequences was systematically analyzed in this study. Our findings showed that the majority of H9N2 AIVs (21 out of 35) belonged to the lineage h9.4.2.5. Most of isolates (33 out of 35) had a PSRSSR↓GLF motif in HA cleavage site. Importantly, 29 out of these 35 isolates had an amino acid exchange (Q226L) in the receptor-binding site. The substitution showed that H9N2 AIVs had the potential affinity to bind to human-like receptor. The currently prevalent H9N2 AIVs in Shandong belonged to the lineage h9.4.2.5 which are different from the vaccine strain SS/94 clade h9.4.2.3. Therefore, the long-term surveillance of H9N2 AIVs is of significance to combat the possible H9N2 AIV outbreaks.
authors.affiliation: College of Life Sciences, Taishan Medical University, Tai'an 271000, China.
authors.forename: Yuxin
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: College of Basic Medicine, Taishan Medical University, Tai'an 271000, China.
authors.forename: Song
authors.initials: S
authors.lastname: Li
authors.affiliation: College of Animal Science and Technology, Shanxi Agricultural University, Taigu 030800, China ; Animal Disease Control Center, Animal Husbandry Bureau of Daiyue, Tai'an 271000, China.
authors.forename: Yufa
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: College of Basic Medicine, Taishan Medical University, Tai'an 271000, China.
authors.forename: Wengang
authors.initials: W
authors.lastname: Song
authors.affiliation: College of Life Sciences, Taishan Medical University, Tai'an 271000, China.
authors.forename: Yujing
authors.initials: Y
authors.lastname: Tang
authors.affiliation: College of Animal Science and Technology, Shanxi Agricultural University, Taigu 030800, China.
authors.forename: Quanhai
authors.initials: Q
authors.lastname: Pang
authors.affiliation: College of Life Sciences, Taishan Medical University, Tai'an 271000, China.
authors.forename: Zengmin
authors.initials: Z
authors.lastname: Miao
mesh_terms: D000596:Amino Acids / Q000235:genetics; D000818:Animals; D001665:Binding Sites / Q000235:genetics; D002645:Chickens / Q000821:virology*; D002681:China; D019143:Evolution, Molecular; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics*; D006388:Hemagglutinins / Q000235:genetics*; D053127:Influenza A Virus, H9N2 Subtype / Q000235:genetics*; D005585:Influenza in Birds / Q000821:virology; D010802:Phylogeny; D011201:Poultry Diseases / Q000821:virology
chemical_list: D000596:Amino Acids; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D006388:Hemagglutinins