title: Systemic BMSC homing in the regeneration of pulp-like tissue and the enhancing effect of stromal cell-derived factor-1 on BMSC homing.
abstract: Pulp regeneration caused by endogenous cells homing has become the new research spot in endodontics. However, the source of functional cells that are involved in and contributed to the reconstituting process has not been identified. In this study, the possible role of systemical BMSC in pulp regeneration and the effect of stromal cell-derived factor-1 (SDF-1) on stem cell recruitment and angiogenesis were evaluated. 54 mice were divided into three groups: SDF-1 group (subcutaneous pockets containing roots with SDF-1 absorbed neutralized collagen gel and the green fluorescent protein (GFP) positive BMSCs transplantation via the tail vein), SDF-1-free group (pockets containing roots with gel alone and GFP + BMSCs transplantation) and Control group (pockets containing roots with gel alone). The animals were sacrificed after the roots were implanted into subcutaneous pockets for 3 weeks. Histomorphometric analysis was performed to evaluate the regenerated tissue in the canal by hematoxylin and eosin (HE) staining. The homing of the transplanted BMSCs was monitored with a fluorescence microscope and immunohistochemical analysis. The expression of ALP in new formed tissue was detected immunohistochemically. Dental-pulp-like tissue and new vessels were regenerated and GFP-positive BMSCs and expression of ALP could be observed in both SDF-1 group and SDF-1-free group. Furthermore, more GFP+ cells, stronger expression of ALP and stronger angiogenesis were found in the SDF-1 group than in the SDF-1-free group. To conclude, systemic BMSC can home to the root canal and participate in dental-pulp-like tissue regeneration. Intracanal application of SDF-1 may enhance BMSC homing efficiency and angiogenesis.
authors.affiliation: Shandong Provincial Key Laboratory of Oral Tissue Regeneration, Shandong University Jinan, Shandong, China ; Jinan Stomatological Hospital Jinan, Shandong, China ; Department of Periodontology, School of Dentistry, Shandong University Jinan, Shandong, China.
authors.forename: Li-Xia
authors.initials: LX
authors.lastname: Zhang
authors.affiliation: Shandong Provincial Key Laboratory of Oral Tissue Regeneration, Shandong University Jinan, Shandong, China ; Liaocheng Peoples Hospital Liaocheng, Shandong, China.
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Shen
authors.affiliation: Shandong Provincial Key Laboratory of Oral Tissue Regeneration, Shandong University Jinan, Shandong, China ; Department of Periodontology, School of Dentistry, Shandong University Jinan, Shandong, China.
authors.forename: Shao-Hua
authors.initials: SH
authors.lastname: Ge
authors.affiliation: Shandong Provincial Key Laboratory of Oral Tissue Regeneration, Shandong University Jinan, Shandong, China ; Department of Periodontology, School of Dentistry, Shandong University Jinan, Shandong, China.
authors.forename: Li-Mei
authors.initials: LM
authors.lastname: Wang
authors.affiliation: Jinan Stomatological Hospital Jinan, Shandong, China.
authors.forename: Xi-Jiao
authors.initials: XJ
authors.lastname: Yu
authors.affiliation: The Affiliated Hospital of Qingdao University Medical College Qingdao, Shandong, China.
authors.forename: Quan-Chen
authors.initials: QC
authors.lastname: Xu
authors.affiliation: Shandong Provincial Key Laboratory of Oral Tissue Regeneration, Shandong University Jinan, Shandong, China ; Department of Periodontology, School of Dentistry, Shandong University Jinan, Shandong, China.
authors.forename: Pi-Shan
authors.initials: PS
authors.lastname: Yang
authors.affiliation: Department of Oral & Maxillofacial Surgery, Qilu Hospital and Institute of Stomatology, Shandong University Jinan, Shandong, China.
authors.forename: Cheng-Zhe
authors.initials: CZ
authors.lastname: Yang
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000502:physiology; D054377:Chemokine CXCL12 / Q000378:metabolism*; D003782:Dental Pulp / Q000378:metabolism*; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D051379:Mice; D012038:Regeneration / Q000502:physiology*
chemical_list: D054377:Chemokine CXCL12
keywords: Pulp regeneration; SDF-1; angiogenesis; bone marrow stromal cells; cell homing