title: On-line restricted access molecularly imprinted solid phase extraction of ivermectin in meat samples followed by HPLC-UV analysis.
abstract: A new restricted access molecularly imprinted polymer coated with bovine serum albumin (RAMIP-BSA) was synthesized, characterized and used for direct analysis of ivermectin from bovine meat samples, in a two-dimensional liquid chromatography system with UV detection. Ivermectin, 4-vynilpiridine and ethylene glycol dimethacrylate were employed as template, functional monomer and cross-linker, respectively. A BSA layer was cross-linked around the polymer, resulting in a biocompatible chemical barrier able to eliminate about 100% of protein from the samples. Ivermectin was extracted from the minced meat samples through a solvent extraction using methanol:water (70:30, v:v), and the extracts were directly injected into the two-dimensional liquid chromatography system, without any other treatment. Samples, fortified with ivermectin from 50 to 500 μg kg(-1), were used to build the analytical calibration curve (r=0.996). The limit of quantification was 50 μg kg(-1). Precision and accuracy presented variation coefficients, as well as relative errors lower than 17.0% and within -18.5% and 22.0%, respectively.
authors.affiliation: Laboratory of Toxicant and Drug Analysis, Faculty of Pharmaceutical Sciences, Gabriel Monteiro da Silva St. 700, Federal University of Alfenas, 37130-000 Alfenas, MG, Brazil.
authors.forename: Marcela Marília
authors.initials: MM
authors.lastname: de Lima
authors.affiliation: Laboratory of Toxicant and Drug Analysis, Faculty of Pharmaceutical Sciences, Gabriel Monteiro da Silva St. 700, Federal University of Alfenas, 37130-000 Alfenas, MG, Brazil.
authors.forename: André Coutinho
authors.initials: AC
authors.lastname: Vieira
authors.affiliation: Laboratory of Toxicant and Drug Analysis, Faculty of Pharmaceutical Sciences, Gabriel Monteiro da Silva St. 700, Federal University of Alfenas, 37130-000 Alfenas, MG, Brazil.
authors.forename: Isarita
authors.initials: I
authors.lastname: Martins
authors.affiliation: Laboratory of Toxicant and Drug Analysis, Faculty of Pharmaceutical Sciences, Gabriel Monteiro da Silva St. 700, Federal University of Alfenas, 37130-000 Alfenas, MG, Brazil.
authors.forename: Vanessa Bergamin
authors.initials: VB
authors.lastname: Boralli
authors.affiliation: Department of Natural Sciences, Federal University of São João del-Rei, 36301-160 São João del-Rei, MG, Brazil.
authors.forename: Keyller Bastos
authors.initials: KB
authors.lastname: Borges
authors.affiliation: Laboratory of Toxicant and Drug Analysis, Faculty of Pharmaceutical Sciences, Gabriel Monteiro da Silva St. 700, Federal University of Alfenas, 37130-000 Alfenas, MG, Brazil. Electronic address: eduardocfig@yahoo.com.br.
authors.forename: Eduardo Costa
authors.initials: EC
authors.lastname: Figueiredo
mesh_terms: D000818:Animals; D002138:Calibration; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005504:Food Analysis / Q000295:instrumentation / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D007559:Ivermectin / Q000032:analysis*; D008460:Meat / Q000032:analysis*; D008689:Methacrylates; D054802:Molecular Imprinting / Q000379:methods*; D011108:Polymers / Q000737:chemistry; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine / Q000737:chemistry; D052616:Solid Phase Extraction / Q000379:methods*; D012997:Solvents / Q000737:chemistry; D014867:Water / Q000737:chemistry
chemical_list: D008689:Methacrylates; D011108:Polymers; D012997:Solvents; D014867:Water; D012710:Serum Albumin, Bovine; D007559:Ivermectin; C004919:ethylene dimethacrylate
keywords: BSA layer; Ivermectin; Meat; RAMIP-BSA; Restricted access molecularly imprinted polymer