title: From Somatic Embryo to Synthetic Seed in Citrus spp. Through the Encapsulation Technology.
abstract: In vitro propagation by somatic embryogenesis represents an efficient alternative method to produce high-quality and healthy plants in Citrus species. The regenerated somatic embryos need protection from mechanical damages during manipulation and transport, as well as nutritive support for their evolution in plantlets after sowing. The encapsulation technology allows to obtain synthetic seeds by covering somatic embryos with a gel of calcium alginate enriched by nutrients. This chapter describes the procedure for producing synthetic seeds containing somatic embryos from different Citrus genotypes.
authors.affiliation: Department of Agricultural, Food and Environmental Sciences, University of Perugia, Borgo XX Giugno 74, Perugia, 06121, Italy. maurizio.micheli@unipg.it.
authors.forename: Maurizio
authors.initials: M
authors.lastname: Micheli
authors.affiliation: Department of Agricultural, Food and Environmental Sciences, University of Perugia, Borgo XX Giugno 74, Perugia, 06121, Italy.
authors.forename: Alvaro
authors.initials: A
authors.lastname: Standardi
mesh_terms: D000464:Alginates / Q000494:pharmacology; D002957:Citrus / Q000235:genetics / Q000254:growth & development*; D004171:Diploidy; D020723:Glucuronic Acid / Q000494:pharmacology; D006603:Hexuronic Acids / Q000494:pharmacology; D063245:Plant Development / Q000235:genetics*; D058439:Plant Somatic Embryogenesis Techniques / Q000379:methods*; D012639:Seeds / Q000235:genetics / Q000254:growth & development; D046509:Tissue Culture Techniques / Q000379:methods*
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D020723:Glucuronic Acid
keywords: Artificial seed; Calcium alginate matrix; Plant tissue culture; Somatic embryo genesis; Synseed