title: Expression of salivary biomarkers in patients with oral mucositis: evaluation by SELDI-TOF/MS.
abstract: OBJECTIVE
  This study aims to evaluate changes in proteomic salivary profile of patients with oral mucositis after adjuvant cancer treatments.
  
  
  METHODS
  Samples were collected from patients after adjuvant cancer therapies, and were analyzed by means of SELDI/TOF. Patients were separated in two groups: patients affected by mucositis (MUCOSITIS) and patient without mucositis (NO MUCOSITIS). All patients were divided in function of the anticancer treatment: patients who had radiotherapy (MUCOSITIS RADIO), had not radiotherapy (MUCOSITIS NO RADIO), had chemotherapy (MUCOSITIS CHEMO), and those who had not chemotherapy (MUCOSITIS NO CHEMO). Statistical evaluation PCA (Principal Component Analysis) was conducted with the software BIO-RAD Data Manager(™) (Version 3.5).
  
  
  RESULTS
  We found the increased peaks of 3443, 3487, and 4135 m/z in MUCOSITIS group, while 6237 m/z was reduced. These same peaks would the same modifications in MUCOSITIS RADIO, while in MUCOSITIS CHEMIO are increased 3443 and 6237 m/z but 3487, 4135 m/z are reduced. These data were confirmed by the PCA.
  
  
  CONCLUSIONS
  Anticancer therapy influenced the level expression of many salivary biomarkers in mucositis with a good significance. Therefore, 3443, 3487, 4135, and 6237 m/z are good biomarker candidates of oral mucositis.
authors.affiliation: Department of Clinical and Experimental Medicine, Foggia University, Foggia, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Ardito
authors.affiliation: Department of Clinical and Experimental Medicine, Foggia University, Foggia, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Giuliani
authors.affiliation: Department of Clinical and Experimental Medicine, Foggia University, Foggia, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Perrone
authors.affiliation: Department of Clinical and Experimental Medicine, Foggia University, Foggia, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Giannatempo
authors.affiliation: Department of Surgical, Oncological and Stomatological Sciences, University of Palermo, Palermo, Italy.
authors.forename: O
authors.initials: O
authors.lastname: Di Fede
authors.affiliation: Department of Odontostomatology and Surgery, University of Bari, Bari, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Favia
authors.affiliation: Department of Surgical, Oncological and Stomatological Sciences, University of Palermo, Palermo, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Campisi
authors.affiliation: Department of Medical, Surgical and Dental Specialties, Second University of Naples, Naples, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Colella
authors.affiliation: Department of Clinical and Experimental Medicine, Foggia University, Foggia, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Lo Muzio
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000032:analysis; D017024:Chemotherapy, Adjuvant / Q000009:adverse effects; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009369:Neoplasms / Q000628:therapy*; D011832:Radiation Injuries / Q000209:etiology / Q000378:metabolism*; D018714:Radiotherapy, Adjuvant / Q000009:adverse effects; D012463:Saliva / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013280:Stomatitis / Q000209:etiology* / Q000378:metabolism*
chemical_list: D015415:Biomarkers
keywords: SELDI-TOF/MS; biomarker discovery; oral mucositis; saliva