title: Translational Control of Autophagy by Orb in the Drosophila Germline.
abstract: Drosophila Orb, the homolog of vertebrate CPEB, is a key translational regulator involved in oocyte polarity and maturation through poly(A) tail elongation of specific mRNAs. orb also has an essential function during early oogenesis that has not been addressed at the molecular level. Here, we show that orb prevents cell death during early oogenesis, thus allowing oogenesis to progress. It does so through the repression of autophagy by directly repressing, together with the CCR4 deadenylase, the translation of Autophagy-specific gene 12 (Atg12) mRNA. Autophagy and cell death observed in orb mutant ovaries are reduced by decreasing Atg12 or other Atg mRNA levels. These results reveal a role of Orb in translational repression and identify autophagy as an essential pathway regulated by Orb during early oogenesis. Importantly, they also establish translational regulation as a major mode of control of autophagy, a key process in cell homeostasis in response to environmental cues.
authors.affiliation: Institut de Génétique Humaine, CNRS UPR1142, 141 rue de la Cardonille, 34396 Montpellier Cedex 5, France.
authors.forename: Patricia
authors.initials: P
authors.lastname: Rojas-Ríos
authors.affiliation: Institut de Génétique Humaine, CNRS UPR1142, 141 rue de la Cardonille, 34396 Montpellier Cedex 5, France.
authors.forename: Aymeric
authors.initials: A
authors.lastname: Chartier
authors.affiliation: Institut de Génétique Humaine, CNRS UPR1142, 141 rue de la Cardonille, 34396 Montpellier Cedex 5, France.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Pierson
authors.affiliation: Montpellier GenomiX, 141 rue de la Cardonille, 34396 Montpellier Cedex 5, France.
authors.forename: Dany
authors.initials: D
authors.lastname: Séverac
authors.affiliation: Montpellier GenomiX, 141 rue de la Cardonille, 34396 Montpellier Cedex 5, France.
authors.forename: Christelle
authors.initials: C
authors.lastname: Dantec
authors.affiliation: Institut de Génétique Humaine, CNRS UPR1142, 141 rue de la Cardonille, 34396 Montpellier Cedex 5, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Busseau
authors.affiliation: Institut de Génétique Humaine, CNRS UPR1142, 141 rue de la Cardonille, 34396 Montpellier Cedex 5, France. Electronic address: martine.simonelig@igh.cnrs.fr.
authors.forename: Martine
authors.initials: M
authors.lastname: Simonelig
mesh_terms: D000818:Animals; D001343:Autophagy / Q000235:genetics*; D000071189:Autophagy-Related Protein-1 Homolog; D002453:Cell Cycle; D016923:Cell Death; D004330:Drosophila / Q000235:genetics* / Q000378:metabolism; D029721:Drosophila Proteins / Q000378:metabolism*; D005260:Female; D018507:Gene Expression Regulation, Developmental; D005854:Germ Cells / Q000378:metabolism; D006706:Homeostasis; D047468:Immunoprecipitation; D009154:Mutation; D009865:Oocytes / Q000378:metabolism; D009866:Oogenesis; D010053:Ovary / Q000378:metabolism; D014176:Protein Biosynthesis; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D012260:Ribonucleases / Q000378:metabolism
chemical_list: D029721:Drosophila Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; C089932:orb protein, Drosophila; C518074:Atg1 protein, Drosophila; D000071189:Autophagy-Related Protein-1 Homolog; D017346:Protein Serine-Threonine Kinases; C501437:CCR4 protein, Drosophila; D012260:Ribonucleases
keywords: Atg genes; CPEB; autophagy; cell death; deadenylation; germline; orb; translational control