title: The effects of self-assembling peptide RADA16 hydrogel on malignant phenotype of human hepatocellular carcinoma cell.
abstract: The aim of this study will provide a self-assembling peptide (RADA16-I) -derived hydrogel as a tool for investigation the malignant phenotype of human hepatocellular carcinoma cell. Characteristic analysis indicated that the peptide consists of a well-defined secondary structure and self-assembly property. Our results showed that these cells cultured in RADA16-I hydrogels showed a spindle-shaped phenotype with irregular and radial nuclei. Immunohistochemical results showed that the expression of fibronectin in hepatocellular carcinoma cells is positive cultured in RADA16-I hydrogels, and the expression levels of laminin are weakly positive. DNA contents cultured in RADA16-I hydrogel gradually increased up to Day 9. The expression levels of VEGFA, EGF and FGF2 in three hydrogels showed no statistically significant differences (P > 0.05), and the expression levels of IGF-1 in RADA16-I and collagen-I were significantly lower than those of in the Matrigel hydrogel (P ≤ 0.05). These findings suggested that the RADA16-I will help to provide a better physiological substrate for hepatocellular carcinoma cell culture, may serve as an ideal model for cancer biology research of tumorigenesis, growth, local invasion, and metastasis.
authors.affiliation: College of Basic Medicine, Zunyi Medical University No. 201 Dalian Road, Huichuan District, Zunyi 563003, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Song
authors.affiliation: College of Basic Medicine, Zunyi Medical University No. 201 Dalian Road, Huichuan District, Zunyi 563003, China.
authors.forename: Yun-Zhu
authors.initials: YZ
authors.lastname: Han
authors.affiliation: College of Basic Medicine, Zunyi Medical University No. 201 Dalian Road, Huichuan District, Zunyi 563003, China.
authors.forename: Guo-Hui
authors.initials: GH
authors.lastname: Cai
authors.affiliation: College of Basic Medicine, Zunyi Medical University No. 201 Dalian Road, Huichuan District, Zunyi 563003, China.
authors.forename: Fu-Shan
authors.initials: FS
authors.lastname: Tang
authors.affiliation: Biomolecular Medicine Lab, West China School of Preclinical Medicine and Forensic Medicine, Sichuan University Chengdu, China.
authors.forename: Ze-Hong
authors.initials: ZH
authors.lastname: Yang
authors.affiliation: College of Basic Medicine, Zunyi Medical University No. 201 Dalian Road, Huichuan District, Zunyi 563003, China.
authors.forename: Di-Shu
authors.initials: DS
authors.lastname: Ao
authors.affiliation: College of Basic Medicine, Zunyi Medical University No. 201 Dalian Road, Huichuan District, Zunyi 563003, China.
authors.forename: An
authors.initials: A
authors.lastname: Zhou
keywords: Self-assembling peptide; hepatocellular carcinoma cell; hydrogel; malignant phenotype; three-dimensional culture