title: Generation of Avian Induced Pluripotent Stem Cells.
abstract: Avian species are among the most diverse vertebrates on our planet and significantly contribute to the balance of the ecology. They are also important food source and serve as a central animal model to decipher developmental biology and disease principles. Derivation of induced pluripotent stem cells (iPSCs) from avian species would enable conservation of genetic diversity as well as offer a valuable cell source that facilitates the use of avian models in many areas of basic and applied research. In this chapter, we describe methods used to successfully reprogram quail fibroblasts into iPSCs by using human transcription factors and the techniques critical to the characterization of their pluripotency.
authors.affiliation: Department of Animal and Dairy Science, Regenerative Bioscience Center, University of Georgia, 425 River Road, RM 316, Athens, GA, 30602, USA.|JRD Biotechnology, Oregon, WI, USA.|State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources Guangxi University, Nanning, China.
authors.forename: Yangqing
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Department of Animal and Dairy Science, Regenerative Bioscience Center, University of Georgia, 425 River Road, RM 316, Athens, GA, 30602, USA. westfranklin1@gmail.com.
authors.forename: Franklin D
authors.initials: FD
authors.lastname: West
authors.affiliation: Department of Animal and Dairy Science, Regenerative Bioscience Center, University of Georgia, 425 River Road, RM 316, Athens, GA, 30602, USA.
authors.forename: Brian J
authors.initials: BJ
authors.lastname: Jordan
authors.affiliation: Department of Animal and Dairy Science, Regenerative Bioscience Center, University of Georgia, 425 River Road, RM 316, Athens, GA, 30602, USA.
authors.forename: Robert B
authors.initials: RB
authors.lastname: Beckstead
authors.affiliation: Department of Animal and Dairy Science, Regenerative Bioscience Center, University of Georgia, 425 River Road, RM 316, Athens, GA, 30602, USA.
authors.forename: Erin T
authors.initials: ET
authors.lastname: Jordan
authors.affiliation: Department of Animal and Dairy Science, Regenerative Bioscience Center, University of Georgia, 425 River Road, RM 316, Athens, GA, 30602, USA. sstice@uga.edu.
authors.forename: Steven L
authors.initials: SL
authors.lastname: Stice
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D065150:Cellular Reprogramming; D058732:Embryoid Bodies; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism*; D005822:Genetic Vectors / Q000235:genetics; D007150:Immunohistochemistry; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism*; D011784:Quail; D014157:Transcription Factors / Q000235:genetics; D014161:Transduction, Genetic; D019076:Transgenes
chemical_list: D014157:Transcription Factors
keywords: Avian; Induced pluripotent stem cell; Quail