title: A Label-Free Photoluminescence Genosensor Using Nanostructured Magnesium Oxide for Cholera Detection.
abstract: Nanomaterial-based photoluminescence (PL) diagnostic devices offer fast and highly sensitive detection of pesticides, DNA, and toxic agents. Here we report a label-free PL genosensor for sensitive detection of Vibrio cholerae that is based on a DNA hybridization strategy utilizing nanostructured magnesium oxide (nMgO; size >30 nm) particles. The morphology and size of the synthesized nMgO were determined by transmission electron microscopic (TEM) studies. The probe DNA (pDNA) was conjugated with nMgO and characterized by X-ray photoelectron and Fourier transform infrared spectroscopic techniques. The target complementary genomic DNA (cDNA) isolated from clinical samples of V. cholerae was subjected to DNA hybridization studies using the pDNA-nMgO complex and detection of the cDNA was accomplished by measuring changes in PL intensity. The PL peak intensity measured at 700 nm (red emission) increases with the increase in cDNA concentration. A linear range of response in the developed PL genosensor was observed from 100 to 500 ng/μL with a sensitivity of 1.306 emi/ng, detection limit of 3.133 ng/μL and a regression coefficient (R(2)) of 0.987. These results show that this ultrasensitive PL genosensor has the potential for applications in the clinical diagnosis of cholera.
authors.affiliation: Biomedical Instrumentation Section, CSIR-National Physical Laboratory, Dr. K. S. Krishnan Marg, New Delhi 110012, India.|Centre for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, New Delhi 110025, India.|Department of Chemistry, College of Arts and Sciences, Oklahoma State University, Stillwater, Oklahoma 74078, United States of America.
authors.forename: Manoj Kumar
authors.initials: MK
authors.lastname: Patel
authors.affiliation: Biomedical Instrumentation Section, CSIR-National Physical Laboratory, Dr. K. S. Krishnan Marg, New Delhi 110012, India.|Department of Electrical and Computer Engineering, Iowa State University, Ames, IA 50011, United States of America.
authors.forename: Md Azahar
authors.initials: MA
authors.lastname: Ali
authors.affiliation: Department of Chemistry, College of Arts and Sciences, Oklahoma State University, Stillwater, Oklahoma 74078, United States of America.
authors.forename: Sadagopan
authors.initials: S
authors.lastname: Krishnan
authors.affiliation: Biomedical Instrumentation Section, CSIR-National Physical Laboratory, Dr. K. S. Krishnan Marg, New Delhi 110012, India.
authors.forename: Ved Varun
authors.initials: VV
authors.lastname: Agrawal
authors.affiliation: Dental Biomaterials Research Chair, Dental Health Department, College of Applied Medical Science, King Saud University, Riyadh, 11437 Saudi Arabia.
authors.forename: AbdulAziz A
authors.initials: AA
authors.lastname: Al Kheraif
authors.affiliation: Dental Biomaterials Research Chair, Dental Health Department, College of Applied Medical Science, King Saud University, Riyadh, 11437 Saudi Arabia.|Biomedical Engineering Department, Faculty of Engineering, Helwan University, 11792, Egypt.
authors.forename: H
authors.initials: H
authors.lastname: Fouad
authors.affiliation: Centre for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, New Delhi 110025, India.
authors.forename: Z A
authors.initials: ZA
authors.lastname: Ansari
authors.affiliation: Centre for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, New Delhi 110025, India.
authors.forename: S G
authors.initials: SG
authors.lastname: Ansari
authors.affiliation: Department of Biotechnology, Delhi Technological University, Shahabad Daulatpur, Main Bawana Road, Delhi 110042, India.
authors.forename: Bansi D
authors.initials: BD
authors.lastname: Malhotra
mesh_terms: D015374:Biosensing Techniques; D002771:Cholera / Q000175:diagnosis* / Q000382:microbiology / Q000473:pathology; D015342:DNA Probes / Q000138:chemical synthesis / Q000737:chemistry; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification*; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D006801:Humans; D057230:Limit of Detection; D008163:Luminescent Measurements; D008277:Magnesium Oxide / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D009693:Nucleic Acid Hybridization / Q000379:methods; D055668:Photochemical Processes; D017550:Spectroscopy, Fourier Transform Infrared; D014734:Vibrio cholerae / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity
chemical_list: D015342:DNA Probes; D004269:DNA, Bacterial; D018076:DNA, Complementary; D008277:Magnesium Oxide