title: Let-7b promotes alpaca hair growth via transcriptional repression of TGFβR I.
abstract: The young male alpaca ear and the back skins were used to investigate the effect of transforming growth factor receptor-β I (TGFβR I) on alpaca hair follicles and hair growth. The expression level and location of TGFβR I in alpaca ear and dorsal skin were detected through real-time quantitative PCR (RT-PCR) and paraffin section immunohistochemical technique (ICC-P). The results shown TGFβR I was lower expression in back skin compared to ear skin and the mean density of the positive reaction in ear skin was significantly higher than back skin. The targeted relationship with let-7b was detected using the dual-luciferase reporter vector of TGFβR I, which showed a significant target relationship between let-7b and TGFβR I. After transfection with let-7b eukaryotic expression vector, the relative mRNA expression of TGFβR I in alpaca skin fibroblasts did not differ, while the relative protein level was significantly decreased. In summary, a higher TGFβR I expression level in the ear skin suggests that TGFβR I may inhibit coat hair elongation. Further studies showed TGFβR I protein was downregulated by let-7b through transcriptional repression.
authors.affiliation: College of Animal Science and Veterinary Medicine, Taigu PC 030801, Shanxi, China.
authors.forename: Shen
authors.initials: S
authors.lastname: Yan
authors.affiliation: College of Animal Science and Veterinary Medicine, Taigu PC 030801, Shanxi, China.
authors.forename: Zhang
authors.initials: Z
authors.lastname: Yu
authors.affiliation: College of Animal Science and Veterinary Medicine, Taigu PC 030801, Shanxi, China.
authors.forename: Liu
authors.initials: L
authors.lastname: Ning
authors.affiliation: College of Animal Science and Veterinary Medicine, Taigu PC 030801, Shanxi, China.
authors.forename: Wang
authors.initials: W
authors.lastname: Hai-Dong
authors.affiliation: College of Animal Science and Veterinary Medicine, Taigu PC 030801, Shanxi, China.
authors.forename: Xie
authors.initials: X
authors.lastname: Jian-Shan
authors.affiliation: College of Animal Science and Veterinary Medicine, Taigu PC 030801, Shanxi, China.
authors.forename: Gao
authors.initials: G
authors.lastname: Shu-Yuan
authors.affiliation: College of Animal Science and Veterinary Medicine, Taigu PC 030801, Shanxi, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Jia-Qi
authors.affiliation: College of Animal Science and Veterinary Medicine, Taigu PC 030801, Shanxi, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Xiu-Ju
authors.affiliation: College of Animal Science and Veterinary Medicine, Taigu PC 030801, Shanxi, China.
authors.forename: Wang
authors.initials: W
authors.lastname: Ting
authors.affiliation: College of Animal Science and Veterinary Medicine, Taigu PC 030801, Shanxi, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Chang-Sheng
authors.affiliation: College of Animal Science and Veterinary Medicine, Taigu PC 030801, Shanxi, China.
authors.forename: He
authors.initials: H
authors.lastname: Xiao-Yan
mesh_terms: D000818:Animals; D002161:Camelids, New World / Q000235:genetics* / Q000254:growth & development; D015536:Down-Regulation; D005347:Fibroblasts / Q000737:chemistry; D005786:Gene Expression Regulation; D006197:Hair / Q000254:growth & development*; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D000077293:Receptor, Transforming Growth Factor-beta Type I; D018125:Receptors, Transforming Growth Factor beta / Q000235:genetics* / Q000378:metabolism; D012867:Skin / Q000378:metabolism
chemical_list: D035683:MicroRNAs; D012333:RNA, Messenger; D018125:Receptors, Transforming Growth Factor beta; D017346:Protein Serine-Threonine Kinases; D000077293:Receptor, Transforming Growth Factor-beta Type I
keywords: Alpaca; Hair growth; Let-7b; Transforming growth factor-β receptor I (TGFβR I)