title: Target genes involved in corticosterone-induced PC12 cell viability and neurite disorders: A potential molecular mechanism of major depressive disorder.
abstract: Past studies confirmed that hypothalamic-pituitary-adrenal (HPA)-axis hormones involved in major depressive disorder (MDD) development. This study used corticosterone treated PC12 cells to explore the potential role of MAPK signal transduction pathway in response to corticosterone stimulation. The results showed that both live cell numbers and cellular neurite outgrowth were remarkably reduced in response to corticosterone treatments. qPCR results demonstrated that the expression levels of four MAPK pathway genes were significantly increased after corticosterone stimulation. In conclusion, glucocorticoids stimulation can affect neuronal cell viability and neurite outgrowth due to the over expression of a group of MAPK pathway genes.
authors.affiliation: Beijing Center for Physical and Chemical Analysis, Beijing 100089, China. Electronic address: mzh5112@126.com.
authors.forename: Mingzhen
authors.initials: M
authors.lastname: Li
authors.affiliation: Beijing Biodonor Biotech Ltd., Beijing 101111, China.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Beijing Center for Physical and Chemical Analysis, Beijing 100089, China.
authors.forename: Jialin
authors.initials: J
authors.lastname: Qian
authors.affiliation: Beijing Center for Physical and Chemical Analysis, Beijing 100089, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Cheng
authors.affiliation: Beijing Center for Physical and Chemical Analysis, Beijing 100089, China.
authors.forename: Huijuan
authors.initials: H
authors.lastname: Wu
authors.affiliation: Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: Beijing Center for Physical and Chemical Analysis, Beijing 100089, China.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Qian
authors.affiliation: Department of Biological Sciences, Southeastern Oklahoma State University, Durant, OK 74701, USA.
authors.forename: Joyce
authors.initials: J
authors.lastname: Su
authors.affiliation: Department of Biological Sciences, Southeastern Oklahoma State University, Durant, OK 74701, USA.
authors.forename: Donald
authors.initials: D
authors.lastname: Wu
authors.affiliation: Department of Biological Sciences, Southeastern Oklahoma State University, Durant, OK 74701, USA.
authors.forename: Larry
authors.initials: L
authors.lastname: Burns
authors.affiliation: Department of Biological Sciences, Southeastern Oklahoma State University, Durant, OK 74701, USA.
authors.forename: Teresa
authors.initials: T
authors.lastname: Golden
authors.affiliation: Department of Biological Sciences, Southeastern Oklahoma State University, Durant, OK 74701, USA. Electronic address: nwu@se.edu.
authors.forename: Ning
authors.initials: N
authors.lastname: Wu
mesh_terms: D000818:Animals; D002452:Cell Count; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects / Q000235:genetics; D003345:Corticosterone / Q000494:pharmacology*; D003865:Major Depressive Disorder / Q000235:genetics* / Q000378:metabolism; D020935:MAP Kinase Signaling System / Q000235:genetics*; D009422:Nervous System Diseases / Q000235:genetics* / Q000378:metabolism; D016501:Neurites / Q000187:drug effects* / Q000378:metabolism; D016716:PC12 Cells; D051381:Rats
chemical_list: D003345:Corticosterone
keywords: MAPK pathway; MKP-1; Signal transduction