title: Male mice are susceptible to high fat diet-induced hyperglycaemia and display increased circulatory retinol binding protein 4 (RBP4) levels and its expression in visceral adipose depots.
abstract: Vitamin A and its metabolites are known to modulate adipose tissue development and its associated complications. Here, we assessed the vitamin A status and its metabolic pathway gene expression in relation to sexual dimorphism by employing 35 days old C57BL/6J male and female mice, which were fed either stock or high fat (HF) diet for 26 weeks. HF diet feeding increased body weight/weight gain and white adipose tissue (WAT) of visceral and subcutaneous regions, however, increase in vitamin A levels observed only in subcutaneous WAT. Further, the expression of most of the vitamin A metabolic pathway genes showed no sexual dimorphism. The observed HF diet-induced hyperglycaemia in male corroborates with increased retinol binding protein 4 (RBP4) levels in plasma and its expression in visceral adipose depots. In conclusion, the male mice are susceptible to high fat diet-induced hyperglycaemia and display higher plasma RBP4 levels, possibly due to its over-expression in visceral adipose depots.
authors.affiliation: a Lipid Biochemistry Division, National Institute of Nutrition , Jamai Osmania, Hyderabad, Telangana , India.
authors.forename: G V
authors.initials: GV
authors.lastname: Asha
authors.affiliation: a Lipid Biochemistry Division, National Institute of Nutrition , Jamai Osmania, Hyderabad, Telangana , India.
authors.forename: M
authors.initials: M
authors.lastname: Raja Gopal Reddy
authors.affiliation: a Lipid Biochemistry Division, National Institute of Nutrition , Jamai Osmania, Hyderabad, Telangana , India.
authors.forename: M
authors.initials: M
authors.lastname: Mahesh
authors.affiliation: a Lipid Biochemistry Division, National Institute of Nutrition , Jamai Osmania, Hyderabad, Telangana , India.
authors.forename: A
authors.initials: A
authors.lastname: Vajreswari
authors.affiliation: a Lipid Biochemistry Division, National Institute of Nutrition , Jamai Osmania, Hyderabad, Telangana , India.
authors.forename: S M
authors.initials: SM
authors.lastname: Jeyakumar
mesh_terms: D000818:Animals; D059305:Diet, High-Fat / Q000009:adverse effects*; D004198:Disease Susceptibility; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D059647:Gene-Environment Interaction; D006943:Hyperglycemia / Q000097:blood / Q000139:chemically induced* / Q000378:metabolism* / Q000473:pathology; D050152:Intra-Abdominal Fat / Q000187:drug effects* / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D054839:Retinol-Binding Proteins, Plasma / Q000378:metabolism*; D012727:Sex Characteristics; D050151:Subcutaneous Fat / Q000187:drug effects / Q000378:metabolism; D014801:Vitamin A / Q000097:blood / Q000378:metabolism
chemical_list: D005227:Fatty Acids; C502487:Rbp4 protein, mouse; D054839:Retinol-Binding Proteins, Plasma; D014801:Vitamin A
keywords: Gene expression; insulin resistance; retinoid; sexual dimorphism