title: Cartilage collagen type II seromarker patterns in axial spondyloarthritis and psoriatic arthritis: associations with disease activity, smoking and HLA-B27.
abstract: The aim of the study was to assess the possible association between type II collagen turnover seromarkers and disease profile in patients with axial spondyloarthritis (SpA) and psoriatic arthritis (PsA). Outpatients with axial SpA (n = 110) or PsA (n = 101) underwent clinical examination including disease activity measures and HLA-B27 typing. The procollagen IIA N-terminal peptide (PIIANP) and a matrix metalloproteinase-generated type II collagen fragment (C2M) were quantified in serum by ELISA. C2M was higher in SpA than in controls, 0.41 versus 0.36 ng/ml (p = 0.004), while PIIANP did not differ between patients and healthy subjects, 2252 versus 2142 ng/ml (p = 0.13). However, DMARD-naïve SpA patients had higher PIIANP, 2461 ng/ml (p = 0.01) and C2M, 0.44 ng/ml (p = 0.0007) levels than controls, and PIIANP correlated with CRP (ρ = 0.34). C2M was lower in SpA smokers, 0.36 ng/ml versus non-smokers, 0.43 ng/ml (p = 0.02), while PIIANP was higher in HLA-B27 positive, 2312 ng/ml versus negative patients, 2021 ng/ml (p = 0.03). In PsA, PIIANP and C2M did not differ between patients and controls, but PIIANP was elevated in patients not receiving DMARDs, 2726 ng/ml. In PsA, PIIANP and C2M did not differ according to smoking and HLA-B27. Cartilage degradation assessed by C2M is increased in SpA irrespective of treatment but not in PsA. Cartilage synthesis reflected by PIIANP is increased in untreated SpA and PsA. PIIANP correlates with CRP in SpA while not in PsA. In DMARD-naïve SpA but not in PsA, HLA-B27 positivity and smoking are associated with a chondro-proliferative metabolic pattern.
authors.affiliation: Department of Rheumatology, Odense University Hospital, Sdr. Boulevard 29, 5000, Odense C, Denmark. heidi.munk@rsyd.dk.
authors.forename: Heidi Lausten
authors.initials: HL
authors.lastname: Munk
authors.affiliation: Rheumatology Biomarkers and Research, Nordic Bioscience, Herlev, Denmark.
authors.forename: Natasja Staehr
authors.initials: NS
authors.lastname: Gudmann
authors.affiliation: Department of Rheumatology, Vejle Hospital, Vejle, Denmark.
authors.forename: Anne Friesgaard
authors.initials: AF
authors.lastname: Christensen
authors.affiliation: Department of Rheumatology, Esbjerg Hospital, Esbjerg, Denmark.
authors.forename: Leif
authors.initials: L
authors.lastname: Ejstrup
authors.affiliation: Institute of Molecular Medicine, University of Southern Denmark, Odense C, Denmark.
authors.forename: Grith Lykke
authors.initials: GL
authors.lastname: Sorensen
authors.affiliation: Department of Rheumatology, Vejle Hospital, Vejle, Denmark.|Department of Rheumatology, Aarhus University Hospital, Aarhus, Denmark.
authors.forename: Anne Gitte
authors.initials: AG
authors.lastname: Loft
authors.affiliation: Rheumatology Biomarkers and Research, Nordic Bioscience, Herlev, Denmark.
authors.forename: Anne C
authors.initials: AC
authors.lastname: Bay-Jensen
authors.affiliation: Rheumatology Biomarkers and Research, Nordic Bioscience, Herlev, Denmark.
authors.forename: Anne Sofie
authors.initials: AS
authors.lastname: Siebuhr
authors.affiliation: Department of Rheumatology, Odense University Hospital, Sdr. Boulevard 29, 5000, Odense C, Denmark.
authors.forename: Peter
authors.initials: P
authors.lastname: Junker
mesh_terms: D000293:Adolescent; D000328:Adult; D015535:Arthritis, Psoriatic / Q000097:blood* / Q000000981:diagnostic imaging / Q000453:epidemiology / Q000276:immunology; D015415:Biomarkers / Q000097:blood; D002356:Cartilage / Q000000981:diagnostic imaging / Q000378:metabolism*; D016022:Case-Control Studies; D024043:Collagen Type II / Q000097:blood*; D003430:Cross-Sectional Studies; D003718:Denmark / Q000453:epidemiology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015796:HLA-B27 Antigen / Q000276:immunology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010446:Peptide Fragments / Q000097:blood*; D010641:Phenotype; D011237:Predictive Value of Tests; D011347:Procollagen / Q000097:blood*; D012307:Risk Factors; D012720:Severity of Illness Index; D012907:Smoking / Q000009:adverse effects* / Q000453:epidemiology; D025241:Spondylarthritis / Q000097:blood* / Q000000981:diagnostic imaging / Q000453:epidemiology / Q000276:immunology; D055815:Young Adult
chemical_list: D015415:Biomarkers; D024043:Collagen Type II; D015796:HLA-B27 Antigen; D010446:Peptide Fragments; D011347:Procollagen; C109195:procollagen type IIA amino-terminal peptide
keywords: Biological markers; Cartilage; Collagen; Psoriatic arthritis; Spondyloarthropathy