title: Discovery and characterization of a first set of polymorphic microsatellite markers in Siganus oramin.
abstract: Nine microsatellite DNA markers were developed and characterized for Siganus oramin by the 5'-anchored polymerase chain reaction technique. A total of 42 alleles were identified in 30 individuals, and the number of alleles per locus ranged from 3 to 7, with an average of 4.7. The observed and expected heterozygosity per locus ranged from 0.5333 to 1.0000 and from 0.5254 to 0.8474, respectively, with an average of 0.7422 and 0.6906, respectively. A significant deviation from the Hardy-Weinberg equilibrium was detected at one microsatellite locus after a Bonferroni's correction (P < 0.0056). No significant linkage disequilibrium was found between any of the pairs of the nine loci. The microsatellite loci developed in this study will improve our understanding of the genetic background of S. oramin.
authors.affiliation: Key Laboratory of East China Sea and Oceanic Fishery Resources Exploitation, Ministry of Agriculture, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai, China.
authors.forename: Y-X
authors.initials: YX
authors.lastname: Liu
authors.affiliation: Key Laboratory of East China Sea and Oceanic Fishery Resources Exploitation, Ministry of Agriculture, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai, China mcy0527@yahoo.com.cn.
authors.forename: C-Y
authors.initials: CY
authors.lastname: Ma
authors.affiliation: Key Laboratory of East China Sea and Oceanic Fishery Resources Exploitation, Ministry of Agriculture, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai, China.
authors.forename: H-Y
authors.initials: HY
authors.lastname: Ma
authors.affiliation: Key Laboratory of East China Sea and Oceanic Fishery Resources Exploitation, Ministry of Agriculture, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai, China.
authors.forename: C-L
authors.initials: CL
authors.lastname: Feng
authors.affiliation: Key Laboratory of East China Sea and Oceanic Fishery Resources Exploitation, Ministry of Agriculture, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai, China.
authors.forename: S-J
authors.initials: SJ
authors.lastname: Li
authors.affiliation: Key Laboratory of East China Sea and Oceanic Fishery Resources Exploitation, Ministry of Agriculture, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai, China.
authors.forename: L-B
authors.initials: LB
authors.lastname: Ma
mesh_terms: D000483:Alleles; D000818:Animals; D000068617:Genetic Background; D056426:Genetic Loci / Q000235:genetics; D006579:Heterozygote; D015810:Linkage Disequilibrium / Q000235:genetics; D018895:Microsatellite Repeats / Q000235:genetics*; D010473:Perciformes / Q000235:genetics*; D011110:Polymorphism, Genetic / Q000235:genetics*