title: Inhibitory effect of imperatorin on insulin-like growth factor-1-induced sebum production in human sebocytes cultured in vitro.
abstract: OBJECTIVE
  Acne is a common skin disease that originates in the sebaceous gland. The pathogenesis of acne is very complex, involving the increase of sebum production and perifollicular inflammation. In this study, we screened the anti-lipogenic material and demonstrated its effect using cultured human sebocytes.
  
  
  METHODS
  Normal human sebocytes were cultured by explanting the sebaceous glands. To evaluate the anti-lipogenic effect, sebocytes were treated with test materials and (14)C-acetate incorporation assay was performed.
  
  
  RESULTS
  To screen the anti-lipogenic materials, we tested the effect of many herbal plant extracts. We found that Angelica dahurica extract inhibited the insulin-like growth factor-1 (IGF-1)-induced sebum production in terms of squalene synthesis in sebocytes. Furthermore, imperatorin isolated from A. dahurica showed remarkable inhibitory effect on squalene production as well as squalene synthase promoter activity. To investigate the putative action mechanism, we tested the effect of imperatorin on intracellular signaling. The results showed that imperatorin inhibited IGF-1-induced phosphorylation of Akt. In addition, imperatorin significantly down-regulated PPAR-γ and SREBP-1, the important transcription factors for lipid synthesis.
  
  
  CONCLUSIONS
  These results suggest that imperatorin has a potential for reducing sebum production in sebocytes, and can be applicable for acne treatment.
authors.affiliation: Department of Dermatology, School of Medicine, Chungnam National University, Daejeon, Republic of Korea; Oriental BioMed Lab, Daejeon, Republic of Korea.
authors.forename: Yul-Lye
authors.initials: YL
authors.lastname: Hwang
authors.affiliation: Department of Dermatology, School of Medicine, Chungnam National University, Daejeon, Republic of Korea.
authors.forename: Myung
authors.initials: M
authors.lastname: Im
authors.affiliation: Oriental BioMed Lab, Daejeon, Republic of Korea.
authors.forename: Min-Ho
authors.initials: MH
authors.lastname: Lee
authors.affiliation: Oriental Medical College of Daejeon University, Daejeon, Republic of Korea.
authors.forename: Seok-Seon
authors.initials: SS
authors.lastname: Roh
authors.affiliation: Department of Chemical & Biological Engineering, Hanbat National University, Daejeon, Republic of Korea.
authors.forename: Byoung Wook
authors.initials: BW
authors.lastname: Choi
authors.affiliation: Department of Dermatology, School of Medicine, Chungnam National University, Daejeon, Republic of Korea.
authors.forename: Sue Jeong
authors.initials: SJ
authors.lastname: Kim
authors.affiliation: Department of Dermatology, School of Medicine, Chungnam National University, Daejeon, Republic of Korea.
authors.forename: Kyung-Cheol
authors.initials: KC
authors.lastname: Sohn
authors.affiliation: Department of Dermatology, School of Medicine, Chungnam National University, Daejeon, Republic of Korea.
authors.forename: Young
authors.initials: Y
authors.lastname: Lee
authors.affiliation: Department of Dermatology, School of Medicine, Chungnam National University, Daejeon, Republic of Korea.
authors.forename: Young-Joon
authors.initials: YJ
authors.lastname: Seo
authors.affiliation: Department of Dermatology, School of Medicine, Chungnam National University, Daejeon, Republic of Korea; Skin Med Company, Daejeon, Republic of Korea.
authors.forename: Jeung-Hoon
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of Dermatology, School of Medicine, Chungnam National University, Daejeon, Republic of Korea. Electronic address: cdkimd@cnu.ac.kr.
authors.forename: Chang Deok
authors.initials: CD
authors.lastname: Kim
mesh_terms: D029969:Angelica / Q000737:chemistry; D002478:Cells, Cultured; D013186:Farnesyl-Diphosphate Farnesyltransferase / Q000037:antagonists & inhibitors / Q000187:drug effects / Q000235:genetics; D011564:Furocoumarins / Q000494:pharmacology*; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000037:antagonists & inhibitors* / Q000494:pharmacology*; D050155:Lipogenesis / Q000187:drug effects; D010766:Phosphorylation / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology; D051057:Proto-Oncogene Proteins c-akt / Q000187:drug effects / Q000378:metabolism; D012627:Sebaceous Glands / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D012629:Sebum / Q000187:drug effects / Q000378:metabolism*; D012867:Skin / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D013185:Squalene / Q000378:metabolism
chemical_list: D011564:Furocoumarins; D010936:Plant Extracts; D007334:Insulin-Like Growth Factor I; D013185:Squalene; D013186:Farnesyl-Diphosphate Farnesyltransferase; D051057:Proto-Oncogene Proteins c-akt; C031534:imperatorin
keywords: Acne; IGF-I; Imperatorin; Sebocytes