title: Platinum nanoparticles encapsulated metal-organic frameworks for the electrochemical detection of telomerase activity.
abstract: A simple and rapid electrochemical sensor is constructed for the detection of telomerase activity based on the electrocatalysis of platinum nanoparticle (Pt NP) encapsulated metal-organic frameworks (MOFs), which are synthesized by one-pot encapsulation of Pt NPs into prototypal MOFs, UiO-66-NH2. Integrating with the efficient electrocatalysis of Pt@MOFs towards NaBH4 oxidation, this biosensor shows the wide dynamic correlation of telomerase activity from 5 × 10(2) to 10(7) HeLa cells mL(-1) and the telomerase activity in a single HeLa cell was calculated to be 2.0 × 10(-11) IU, providing a powerful platform for detecting telomerase activity.
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, P. R. China. jpl@nju.edu.cn.
authors.forename: Pinghua
authors.initials: P
authors.lastname: Ling
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, P. R. China. jpl@nju.edu.cn.
authors.forename: Jianping
authors.initials: J
authors.lastname: Lei
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, P. R. China. jpl@nju.edu.cn.
authors.forename: Li
authors.initials: L
authors.lastname: Jia
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, P. R. China. jpl@nju.edu.cn.
authors.forename: Huangxian
authors.initials: H
authors.lastname: Ju
mesh_terms: D055664:Electrochemical Techniques / Q000379:methods*; D053768:Metal Nanoparticles; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D009930:Organic Chemicals / Q000737:chemistry*; D010984:Platinum / Q000737:chemistry*; D019098:Telomerase / Q000378:metabolism*
chemical_list: D009930:Organic Chemicals; D010984:Platinum; D019098:Telomerase