title: Molecular diversity of turncurtoviruses in Iran.
abstract: Turnip curly top virus (TCTV) is the only member of the newly established genus Turncurtovirus (family Geminiviridae). As part of an ongoing study to identify additional plant hosts and the diversity of turncurtoviruses, between 2012 and 2014, we sampled symptomatic turnip plants and other crops in the provinces Fars and Khorasan Razavi (southern and northeastern Iran, respectively). Infection by turncurtoviruses was tested by PCR and/or rolling-circle amplification (RCA) coupled with restriction enzyme digests. Turncurtoviruses were identified in turnip as well as seven other field crops, including eggplant, basil, radish, lettuce, sugar beet, red beet and spinach. Full turncurtovirus genomes were recovered from 25 of these samples, leading to the identification of TCTV and a new putative turncurtovirus, turnip leaf roll virus (TLRV; 13 isolates), which shares <80% genome-wide pairwise identity with TCTV. Agroinoculation of plants with an infectious clone of TLRV demonstrated that this virus could infect several plant hosts under greenhouse conditions and could be transmitted by the leafhopper Circulifer haematoceps (Mulsant and Rey, 1855) from agroinoculated to healthy plants.
authors.affiliation: Department of Plant Protection, College of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Kamali
authors.affiliation: Department of Plant Protection, College of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran. jheydarnejad@uk.ac.ir.
authors.forename: Jahangir
authors.identifier: http://orcid.org/0000-0002-9229-3001
authors.initials: J
authors.lastname: Heydarnejad
authors.affiliation: Department of Plant Protection, College of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran.
authors.forename: Hossain
authors.initials: H
authors.lastname: Massumi
authors.affiliation: Department of Plant Biology, Uppsala BioCenter, Linnean Center of Plant Biology in Uppsala, Swedish University of Agricultural Sciences, Box 7080, 750 07, Uppsala, Sweden.
authors.forename: Anders
authors.initials: A
authors.lastname: Kvarnheden
authors.affiliation: School of Biological Sciences and Biomolecular Interaction Centre, University of Canterbury, Private Bag 4800, Christchurch, 8140, New Zealand.
authors.forename: Simona
authors.initials: S
authors.lastname: Kraberger
authors.affiliation: School of Biological Sciences and Biomolecular Interaction Centre, University of Canterbury, Private Bag 4800, Christchurch, 8140, New Zealand.|Department of Plant Pathology and Emerging Pathogens Institute, University of Florida, Gainesville, FL, 32611, USA.|Structural Biology Research Unit, Department of Clinical Laboratory Sciences, University of Cape Town, Rondebosch, 7701, Cape Town, South Africa.
authors.forename: Arvind
authors.initials: A
authors.lastname: Varsani
mesh_terms: D016000:Cluster Analysis; D018556:Crops, Agricultural / Q000821:virology*; D017791:Geminiviridae / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D014644:Genetic Variation; D016679:Genome, Viral; D007492:Iran; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D012367:RNA, Viral / Q000235:genetics; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
chemical_list: D012367:RNA, Viral