title: Messenger RNA profile analysis deciphers new Esrrb responsive genes in prostate cancer cells.
abstract: BACKGROUND
  Orphan nuclear receptor estrogen related receptor β (Esrrb or ERRβ) is well known in stem cells and early embryonic development. However, little is known about its function in cancer.
  
  
  METHODS
  We investigated the mRNA profile alterations induced by Esrrb expression and its synthetic ligand DY131 in human prostate cancer DU145 cells via RNA-Seq analysis.
  
  
  RESULTS
  We distinguished 67 mRNAs differentially expressed by Esrrb alone. Although DY131 alone did not change any gene, treatment of DY131 in the presence of Esrrb altered 1161 mRNAs. These observations indicated Esrrb had both ligand-independent and ligand-dependent activity. When Esrrb was expressed, DY131 treatment further regulated 15 Esrrb-altered mRNAs. DY131 acted as an antagonist for 11 of 15 mRNAs (wdr52, f13a1, pxdn, spns2, loc100506599, tagln, loc441454, tkel1, sema3f, zcwpw2, sdc2) and as an agonist for 4 of the 15 mRNAs (rarres3, oasl, padi2, ddx60). Gene ontology analyses showed altered genes are related to transcription and translation regulation, cell proliferation and apoptosis regulation, and cellular metabolism.
  
  
  CONCLUSIONS
  Our results characterized mRNA profiles in DU145 prostate cancer cells driven by Esrrb expression and Esrrb ligand DY131, and provided multiple markers to characterize Esrrb's function in Esrrb research.
authors.affiliation: Department of Biochemistry, University of Missouri, Columbia, MO, 65211, USA. y_l54@txstate.edu.|MU Center for Botanical Interaction Studies, University of Missouri, Columbia, MO, 65211, USA. y_l54@txstate.edu.|Xiphophorus Genetic Stock Center, Texas State University, San Marcos, TX, 78666, USA. y_l54@txstate.edu.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Lu
authors.affiliation: MU Center for Botanical Interaction Studies, University of Missouri, Columbia, MO, 65211, USA. jl482@mail.missouri.edu.|Computer Science Department, University of Missouri, Columbia, MO, 65211, USA. jl482@mail.missouri.edu.|Informatics Institute, University of Missouri, Columbia, MO, 65211, USA. jl482@mail.missouri.edu.
authors.forename: Jilong
authors.initials: J
authors.lastname: Li
authors.affiliation: MU Center for Botanical Interaction Studies, University of Missouri, Columbia, MO, 65211, USA. chengji@missouri.edu.|Computer Science Department, University of Missouri, Columbia, MO, 65211, USA. chengji@missouri.edu.|Informatics Institute, University of Missouri, Columbia, MO, 65211, USA. chengji@missouri.edu.
authors.forename: Jianlin
authors.initials: J
authors.lastname: Cheng
authors.affiliation: Department of Biochemistry, University of Missouri, Columbia, MO, 65211, USA. lubahnd@missouri.edu.|MU Center for Botanical Interaction Studies, University of Missouri, Columbia, MO, 65211, USA. lubahnd@missouri.edu.
authors.forename: Dennis B
authors.initials: DB
authors.lastname: Lubahn
mesh_terms: D017209:Apoptosis / Q000235:genetics; D001483:Base Sequence; D063646:Carcinogenesis / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D053595:Embryonic Stem Cells / Q000378:metabolism; D004734:Energy Metabolism / Q000235:genetics; D020869:Gene Expression Profiling; D005819:Genetic Markers / Q000235:genetics; D057809:HEK293 Cells; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D008297:Male; D011471:Prostatic Neoplasms / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*; D011960:Receptors, Estrogen / Q000235:genetics*; D017423:Sequence Analysis, RNA; D014158:Transcription, Genetic / Q000235:genetics
chemical_list: C490918:ESRRB protein, human; D005819:Genetic Markers; D012333:RNA, Messenger; D011960:Receptors, Estrogen