title: Demethylation of miR-10b plays a suppressive role in ccRCC cells.
abstract: MicroRNAs have been implicated in cancer cells proliferation, migration and invasion, including clear-cell renal cell carcinoma (ccRCC). DNA methylation, a major epigenetic change associated with cancer, may lead to transcriptional silencing of tumor suppressor genes, including miRNAs, which may be a possible mechanism in carcinogenesis. In this study, we aim to investigate the role of miR-10b in ccRCC and its possible epigenetic mechanism. By qPCR and MSP, we found that miR-10b was significantly decreased in ccRCC tissues and cells, and exhibited heavy methylation on promoter. Upregulation of miR-10b by transfecting with lentivirus highly expressed miR-10b or by exogenous demethylation agents was capable of inhibiting cell proliferation, migration and invasion measured by CCK-8 cell proliferation assay, scratch assay, transwell assay, and flow cytometric analysis of the cell cycle. Our results suggest that miR-10b plays a tumor-suppressive role in ccRCC. Demethylation of miR-10b may be therapeutically beneficial for ccRCC treatment.
authors.affiliation: Department of Urology, The Second Xiangya Hospital, Central South University Changsha 410011, Hunan, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: He
authors.affiliation: Department of Urology, The Second Xiangya Hospital, Central South University Changsha 410011, Hunan, China.
authors.forename: Xiaokun
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Department of Urology, The Second Xiangya Hospital, Central South University Changsha 410011, Hunan, China.
authors.forename: Hongyi
authors.initials: H
authors.lastname: Jiang
authors.affiliation: Department of Urology, The Second Xiangya Hospital, Central South University Changsha 410011, Hunan, China.
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Zhong
authors.affiliation: Department of Urology, The Second Xiangya Hospital, Central South University Changsha 410011, Hunan, China.
authors.forename: Ran
authors.initials: R
authors.lastname: Xu
mesh_terms: D001374:Azacitidine / Q000031:analogs & derivatives / Q000494:pharmacology; D002292:Carcinoma, Renal Cell / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D002453:Cell Cycle; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D018899:CpG Islands; D019175:DNA Methylation / Q000187:drug effects; D015254:DNA Modification Methylases / Q000037:antagonists & inhibitors / Q000378:metabolism; D000077209:Decitabine; D004791:Enzyme Inhibitors / Q000494:pharmacology; D044127:Epigenesis, Genetic / Q000187:drug effects; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007680:Kidney Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D009361:Neoplasm Invasiveness; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D010654:Phenylbutyrates / Q000494:pharmacology; D011401:Promoter Regions, Genetic; D013997:Time Factors; D014162:Transfection; D051879:Twist-Related Protein 1 / Q000235:genetics / Q000378:metabolism
chemical_list: D004791:Enzyme Inhibitors; C547183:MIRN10 microRNA, human; D035683:MicroRNAs; D009687:Nuclear Proteins; D010654:Phenylbutyrates; C103173:TWIST1 protein, human; D051879:Twist-Related Protein 1; D000077209:Decitabine; C075773:4-phenylbutyric acid; D015254:DNA Modification Methylases; D001374:Azacitidine
keywords: Clear-cell renal cell carcinoma; epigenetic; invasion; methylation; miR-10b; microRNA