title: A panel of four decreased serum microRNAs as a novel biomarker for early Parkinson's disease.
abstract: BACKGROUND
  Sensitive, non-invasive biomarkers that facilitate Parkinson's disease (PD) detection and stage assignment are currently unavailable.
  
  
  OBJECTIVE
  The objective of this study is to investigate the potential of circulating microRNAs (miRNAs) as novel biomarkers for PD.
  
  
  METHODS
  Solexa sequencing technology and quantitative real-time PCR were applied to screen and verify altered serum miRNAs in PD patients.
  
  
  RESULTS
  Serum miR-141, miR-214, miR-146b-5p, and miR-193a-3p were decreased significantly in PD patients compared with controls. Furthermore, the 4-miRNA panel enabled the differentiation of HY stage 1 and 2 PD patients from controls.
  
  
  CONCLUSIONS
  The four serum miRNAs may represent novel biomarkers for the early detection of PD.
authors.affiliation: a State Key Laboratory of Pharmaceutical Biotechnology, Nanjing Advanced Institute for Life Sciences, Nanjing University School of Life Sciences, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, Nanjing University , Nanjing , China .
authors.forename: Hui
authors.initials: H
authors.lastname: Dong
authors.affiliation: a State Key Laboratory of Pharmaceutical Biotechnology, Nanjing Advanced Institute for Life Sciences, Nanjing University School of Life Sciences, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, Nanjing University , Nanjing , China .|b Department of Clinical Laboratory , Jinling Hospital, Nanjing University School of Medicine , Nanjing , China , and.
authors.forename: Cheng
authors.initials: C
authors.lastname: Wang
authors.affiliation: a State Key Laboratory of Pharmaceutical Biotechnology, Nanjing Advanced Institute for Life Sciences, Nanjing University School of Life Sciences, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, Nanjing University , Nanjing , China .
authors.forename: Sunbin
authors.initials: S
authors.lastname: Lu
authors.affiliation: c Department of Neurology , Affiliated Brain Hospital of Nanjing Medical University , Nanjing , China.
authors.forename: Cuiyu
authors.initials: C
authors.lastname: Yu
authors.affiliation: a State Key Laboratory of Pharmaceutical Biotechnology, Nanjing Advanced Institute for Life Sciences, Nanjing University School of Life Sciences, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, Nanjing University , Nanjing , China .
authors.forename: Lei
authors.initials: L
authors.lastname: Huang
authors.affiliation: c Department of Neurology , Affiliated Brain Hospital of Nanjing Medical University , Nanjing , China.
authors.forename: Wuruo
authors.initials: W
authors.lastname: Feng
authors.affiliation: c Department of Neurology , Affiliated Brain Hospital of Nanjing Medical University , Nanjing , China.
authors.forename: Hui
authors.initials: H
authors.lastname: Xu
authors.affiliation: a State Key Laboratory of Pharmaceutical Biotechnology, Nanjing Advanced Institute for Life Sciences, Nanjing University School of Life Sciences, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, Nanjing University , Nanjing , China .
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.affiliation: a State Key Laboratory of Pharmaceutical Biotechnology, Nanjing Advanced Institute for Life Sciences, Nanjing University School of Life Sciences, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, Nanjing University , Nanjing , China .
authors.forename: Ke
authors.initials: K
authors.lastname: Zen
authors.affiliation: a State Key Laboratory of Pharmaceutical Biotechnology, Nanjing Advanced Institute for Life Sciences, Nanjing University School of Life Sciences, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, Nanjing University , Nanjing , China .
authors.forename: Qiao
authors.initials: Q
authors.lastname: Yan
authors.affiliation: c Department of Neurology , Affiliated Brain Hospital of Nanjing Medical University , Nanjing , China.
authors.forename: Weiguo
authors.initials: W
authors.lastname: Liu
authors.affiliation: a State Key Laboratory of Pharmaceutical Biotechnology, Nanjing Advanced Institute for Life Sciences, Nanjing University School of Life Sciences, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, Nanjing University , Nanjing , China .|b Department of Clinical Laboratory , Jinling Hospital, Nanjing University School of Medicine , Nanjing , China , and.
authors.forename: Chunni
authors.initials: C
authors.lastname: Zhang
authors.affiliation: a State Key Laboratory of Pharmaceutical Biotechnology, Nanjing Advanced Institute for Life Sciences, Nanjing University School of Life Sciences, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, Nanjing University , Nanjing , China .
authors.forename: Chen-Yu
authors.initials: CY
authors.lastname: Zhang
mesh_terms: D000368:Aged; D015415:Biomarkers / Q000097:blood*; D042241:Early Diagnosis; D005260:Female; D015870:Gene Expression; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood* / Q000235:genetics; D008875:Middle Aged; D010300:Parkinson Disease / Q000097:blood* / Q000175:diagnosis* / Q000235:genetics; D011379:Prognosis; D012372:ROC Curve; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012720:Severity of Illness Index
chemical_list: D015415:Biomarkers; C533895:MIRN141 microRNA, human; C542542:MIRN146 microRNA, human; C546818:MIRN193 microRNA, human; C547397:MIRN214 microRNA, human; D035683:MicroRNAs
keywords: Biomarker; Parkinson’s disease; RT-qPCR; serum microRNA