title: Effects of a discoloration-resistant calcium aluminosilicate cement on the viability and proliferation of undifferentiated human dental pulp stem cells.
abstract: Discoloration-resistant calcium aluminosilicate cement has been formulated to overcome the timely problem of tooth discoloration reported in the clinical application of bismuth oxide-containing hydraulic cements. The present study examined the effects of this experimental cement (Quick-Set2) on the viability and proliferation of human dental pulp stem cells (hDPSCs) by comparing the cellular responses with commercially available calcium silicate cement (white mineral trioxide aggregate; WMTA) after different aging periods. Cell viability and proliferation were examined using assays that examined plasma membrane integrity, leakage of cytosolic enzyme, caspase-3 activity for early apoptosis, oxidative stress, mitochondrial metabolic activity and intracellular DNA content. Results of the six assays indicated that both Quick-Set2 and WMTA were initially cytotoxic to hDPSCs after setting for 24 h, with Quick-Set2 being comparatively less cytotoxic than WMTA at this stage. After two aging cycles, the cytotoxicity profiles of the two hydraulic cements were not significantly different and were much less cytotoxic than the positive control (zinc oxide-eugenol cement). Based on these results, it is envisaged that any potential beneficial effect of the discoloration-resistant calcium aluminosilicate cement on osteogenesis by differentiated hDPSCs is more likely to be revealed after outward diffusion and removal of its cytotoxic components.
authors.affiliation: State Key Laboratory of Military Stomatology, School of Stomatology, Department of Prosthodontics, Fourth Military Medical University, Xi'an, Shaanxi, China.|Department of Endodontics, Georgia Regents University, Augusta, Georgia, USA.
authors.forename: Li-na
authors.initials: LN
authors.lastname: Niu
authors.affiliation: Department of Endodontics, Georgia Regents University, Augusta, Georgia, USA.
authors.forename: Devon
authors.initials: D
authors.lastname: Watson
authors.affiliation: Department of Endodontics, Georgia Regents University, Augusta, Georgia, USA.
authors.forename: Kyle
authors.initials: K
authors.lastname: Thames
authors.affiliation: LECOM School of Dental Medicine, Bradenton, Florida, USA.
authors.forename: Carolyn M
authors.initials: CM
authors.lastname: Primus
authors.affiliation: Department of Endodontics, Georgia Regents University, Augusta, Georgia, USA.
authors.forename: Brian E
authors.initials: BE
authors.lastname: Bergeron
authors.affiliation: State Key Laboratory of Military Stomatology, School of Stomatology, Department of Prosthodontics, Fourth Military Medical University, Xi'an, Shaanxi, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Jiao
authors.affiliation: Department of Dentistry, Federal University of Santa Catarina, Florianópolis, Santa Catarina, Brazil.
authors.forename: Eduardo A
authors.initials: EA
authors.lastname: Bortoluzzi
authors.affiliation: Department of Periodontics, Georgia Regents University, Augusta, Georgia, USA.
authors.forename: Christopher W
authors.initials: CW
authors.lastname: Cutler
authors.affiliation: State Key Laboratory of Military Stomatology, School of Stomatology, Department of Prosthodontics, Fourth Military Medical University, Xi'an, Shaanxi, China.
authors.forename: Ji-hua
authors.initials: JH
authors.lastname: Chen
authors.affiliation: Department of Oral Biology, Georgia Regents University, Augusta, Georgia, USA.
authors.forename: David H
authors.initials: DH
authors.lastname: Pashley
authors.affiliation: Department of Endodontics, Georgia Regents University, Augusta, Georgia, USA.
authors.forename: Franklin R
authors.initials: FR
authors.lastname: Tay
mesh_terms: D000293:Adolescent; D000328:Adult; D000538:Aluminum Silicates / Q000494:pharmacology; D053148:Caspase 3 / Q000378:metabolism; D002454:Cell Differentiation; D002462:Cell Membrane / Q000378:metabolism; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D000077215:Clay; D003738:Dental Cements / Q000494:pharmacology; D003782:Dental Pulp / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D016130:Immunophenotyping; D018384:Oxidative Stress; D010641:Phenotype; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D055815:Young Adult
chemical_list: D000538:Aluminum Silicates; D003738:Dental Cements; D053148:Caspase 3; D000077215:Clay