title: Electron-induced dissociation (EID) for structure characterization of glycerophosphatidylcholine: determination of double-bond positions and localization of acyl chains.
abstract: Glycerophospholipids are a highly abundant and diverse collection of biologically relevant lipids, and distinction between isomeric and isobaric species is a fundamental aspect for confident identification. The ability to confidently assign a unique structure to a glycerophospholipid of interest is dependent on determining the number and location of the points of unsaturation and assignment of acyl chain position. The use of high-energy electrons (>20 eV) to induce gas-phase dissociation of intact precursor ions results in diagnostic product ions for localizing double-bond positions and determining acyl chain assignment. We describe a high-resolution, tandem mass spectrometry method for structure characterization of glycerophospholipids using electron-induced dissociation (EID). Furthermore, the inclusion of nomenclature to systematically assign bond cleavage sites with acyl chain position and double-bond location enables a uniform platform for lipid identification. The EID methodology detailed here combines novel application of an electron-based dissociation technique with high-resolution mass spectrometry that facilitates a new experimental approach for lipid biomarker discovery and validation.
authors.affiliation: Department of Pharmaceutical Sciences, University of Maryland, School of Pharmacy, Baltimore, MD, USA.
authors.forename: Jace W
authors.initials: JW
authors.lastname: Jones
authors.affiliation: Bruker Daltonics, Billerica, MA, USA.
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Thompson
authors.affiliation: Department of Pharmaceutical Sciences, University of Maryland, School of Pharmacy, Baltimore, MD, USA.
authors.forename: Claire L
authors.initials: CL
authors.lastname: Carter
authors.affiliation: Department of Pharmaceutical Sciences, University of Maryland, School of Pharmacy, Baltimore, MD, USA.
authors.forename: Maureen A
authors.initials: MA
authors.lastname: Kane
mesh_terms: D001671:Biochemistry; D005997:Glycerylphosphorylcholine / Q000032:analysis* / Q000737:chemistry*; D008968:Molecular Conformation; D053719:Tandem Mass Spectrometry
chemical_list: D005997:Glycerylphosphorylcholine
keywords: FT-ICR; electron-induced dissociation; lipidomics; tandem mass spectrometry