title: Baicalin ameliorates isoproterenol-induced acute myocardial infarction through iNOS, inflammation and oxidative stress in rat.
abstract: Baicalin belongs to glucuronic acid glycosides and after hydrolysis baicalein and glucuronic acid come into being. It has such effects as clearing heat and removing toxicity, anti-inflammation, choleresis, bringing high blood pressure down, diuresis, anti-allergic reaction and so on. In this study, we investigated whether baicalin ameliorates isoproterenol-induced acute myocardial infarction and its mechanism. Rat model of acute myocardial infarction was induced by isoproterenol. Casein kinase (CK), the MB isoenzyme of creatine kinase (CK-MB), lactate dehydrogenase (LDH), cardiac troponin T (cTnT) and infarct size measurement were used to measure the protective effect of baicalin on isoproterenol-induced acute myocardial infarction. iNOS protein expression in rat was analyzed using western blot analysis. Tumor necrosis factor-alpha (TNF-α), interleukin 6 (IL-6), malondialdehyde (MDA) and superoxide dismutase (SOD) and caspase-3 activation levels were explored using commercial ELISA kits. In the acute myocardial infarction experiment, baicalin effectively ameliorates the level of CK, CK-MB, LDH and cTnT, reduced infarct size in acute myocardial infarction rat model. Meanwhile, treatment with baicalin effectively decreased the iNOS protein expression, inflammatory factors and oxidative stresses in a rat model of acute myocardial infarction. However, baicalin emerged that anti-apoptosis activity and suppressed the activation of caspase-3 in a rat model of acute myocardial infarction. The data suggest that the protective effect of baicalin ameliorates isoproterenol-induced acute myocardial infarction through iNOS, inflammation and oxidative stress in rat.
authors.affiliation: Department of Emergency, Shanghai Tenth People's Hospital, Tongji University School of Medicine 301 Yanchang Road, Shanghai 200072, China.
authors.forename: Huaguo
authors.initials: H
authors.lastname: Chen
authors.affiliation: Department of Emergency, Shanghai Tenth People's Hospital, Tongji University School of Medicine 301 Yanchang Road, Shanghai 200072, China.
authors.forename: Yongfu
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Emergency, Shanghai Tenth People's Hospital, Tongji University School of Medicine 301 Yanchang Road, Shanghai 200072, China.
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Emergency, Shanghai Tenth People's Hospital, Tongji University School of Medicine 301 Yanchang Road, Shanghai 200072, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhao
authors.affiliation: Department of Emergency, Shanghai Tenth People's Hospital, Tongji University School of Medicine 301 Yanchang Road, Shanghai 200072, China.
authors.forename: Huihui
authors.initials: H
authors.lastname: Ruan
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D047388:Casein Kinases / Q000097:blood; D053148:Caspase 3 / Q000378:metabolism; D052279:Creatine Kinase, MB Form / Q000097:blood; D004791:Enzyme Inhibitors / Q000494:pharmacology / Q000627:therapeutic use*; D005419:Flavonoids / Q000494:pharmacology / Q000627:therapeutic use*; D007249:Inflammation / Q000188:drug therapy / Q000378:metabolism; D015850:Interleukin-6 / Q000097:blood; D007545:Isoproterenol; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D009203:Myocardial Infarction / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000097:blood; D020107:Troponin T / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D004791:Enzyme Inhibitors; D005419:Flavonoids; D015850:Interleukin-6; D020107:Troponin T; D014409:Tumor Necrosis Factor-alpha; C038044:baicalin; D008315:Malondialdehyde; D007770:L-Lactate Dehydrogenase; D052247:Nitric Oxide Synthase Type II; D013482:Superoxide Dismutase; D047388:Casein Kinases; D052279:Creatine Kinase, MB Form; D053148:Caspase 3; D007545:Isoproterenol
keywords: Baicalin; INOS; acute myocardial infarction; inflammation; isoproterenol; oxidative stress