title: EFEMP2 is upregulated in gliomas and promotes glioma cell proliferation and invasion.
abstract: Gliomas are the most common and aggressive form of primary brain tumor. Although EGF-containing fibulin-like extracellular matrix protein 2 (EFEMP2), an extracellular matrix (ECM) glycoprotein, is regarded as a candidate oncogene, little is known about the association of EFEMP2 and gliomas. Here, the expression of EFEMP2 was significantly increased in glioma tissues (n=60) compared to non-tumorous brain tissues (n=25). Silencing of EFEMP2 expression through RNA interference in two glioma cell lines (U87 and U373) remarkably inhibited cell proliferation and G1/S transition. More importantly, EFEMP2 silencing significantly induced cell apoptosis via increasing the ratio of Bax and Bcl-2. Additionally, knockdown of EFEMP2 significantly inhibited the invasive ability of both glioma cells, which was associated with the downregulated expression of metalloproteinase-2 (MMP-2) and MMP-9. In conclusion, expression of EFEMP2 was associated with the oncogenic potential of gliomas and silencing of its expression can suppress cancer cell growth and metastasis. Inhibition of EFEMP2 may be a therapeutic strategy for gliomas.
authors.affiliation: Department of Neurosurgery, Renmin Hospital of Wuhan University Wuhan, China.
authors.forename: Long
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, Renmin Hospital of Wuhan University Wuhan, China.
authors.forename: Qianxue
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Department of Neurosurgery, Renmin Hospital of Wuhan University Wuhan, China.
authors.forename: Zhibiao
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Department of Neurosurgery, Renmin Hospital of Wuhan University Wuhan, China.
authors.forename: Daofeng
authors.initials: D
authors.lastname: Tian
authors.affiliation: Department of Neurosurgery, Renmin Hospital of Wuhan University Wuhan, China.
authors.forename: Haitao
authors.initials: H
authors.lastname: Xu
authors.affiliation: Department of Neurosurgery, Renmin Hospital of Wuhan University Wuhan, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Cai
authors.affiliation: Department of Neurosurgery, Renmin Hospital of Wuhan University Wuhan, China.
authors.forename: Baohui
authors.initials: B
authors.lastname: Liu
authors.affiliation: Department of Neurosurgery, Renmin Hospital of Wuhan University Wuhan, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Deng
mesh_terms: D017209:Apoptosis / Q000502:physiology; D015153:Blotting, Western; D001932:Brain Neoplasms / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000502:physiology; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis*; D005434:Flow Cytometry; D055785:Gene Knockdown Techniques; D005910:Glioma / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D009361:Neoplasm Invasiveness / Q000473:pathology*; D016133:Polymerase Chain Reaction; D034741:RNA, Small Interfering; D014162:Transfection; D015854:Up-Regulation
chemical_list: C403672:EFEMP2 protein, human; D016326:Extracellular Matrix Proteins; D034741:RNA, Small Interfering
keywords: Apoptosis; EFEMP2; G1/S transition; gliomas; metalloproteinase