title: MTSS1 gene regulated by miR-96 inhibits cell proliferation and metastasis in tongue squamous cellular carcinoma Tca8113 cell line.
abstract: BACKGROUND
  Metastasis suppressor-1 (MTSS1) is a novel potential metastasis suppressor gene in several types of human cancers. However, the exact function and regulatory mechanism of MTSS1 in Tongue squamous cellular carcinoma (TSCC) have not been elucidated.
  
  
  METHODS
  We first confirmed the MTSS1 gene expression by using quantitative real time-PCR (qRT-PCR) and immunohistochemical staining. Then we detected the effect of MTSS1 gene on Tca8113 cells proliferation and invasion ability by using MTT, wound healing and invasion assay. Finally by using bioinformatics analysis, luciferase reporter assay and a serial method, we analyzed the targeting of miR-96 on MTSS1 and the ability of miR-96 on MTSS1 gene mediated biological alterations in Tca8113 cells.
  
  
  RESULTS
  Our findings showed that the expression of MTSS1 was down-regulated in both TSCC tissues and Tca8113 cells. Forced expression of MTSS1 led to inhibited cell proliferation ability, retarded wound closing and reduced trans-membrane cell numbers. MiR-96 is confirmed to be a direct target of MTSS1 gene and could regulate MTSS1 mediated Tca8113 cells proliferation and metastasis. But miR-96 could not completely restore the invasion ability of Tca8113 cells.
  
  
  CONCLUSIONS
  MiR-96 targeting and promoting MTSS1 repression may precipitate in the TSCC tumorigenesis through bypassing cell proliferation and metastasis control.
authors.affiliation: Key Laboratory of Liaoning Province Oral Disease, School of Stomatology, China Medical University Shenyang 110002, P. R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Department of Pathology, School of Stomatology, China Medical University Shenyang 110002, P. R. China.
authors.forename: Mei-Si
authors.initials: MS
authors.lastname: Ren
authors.affiliation: Department of Neurobiology, China Medical University Shenyang 110001, P. R. China.
authors.forename: Chao
authors.initials: C
authors.lastname: Shang
authors.affiliation: Key Laboratory of Liaoning Province Oral Disease, School of Stomatology, China Medical University Shenyang 110002, P. R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhu
authors.affiliation: Key Laboratory of Liaoning Province Oral Disease, School of Stomatology, China Medical University Shenyang 110002, P. R. China ; Department of Pathology, School of Stomatology, China Medical University Shenyang 110002, P. R. China.
authors.forename: Ming
authors.initials: M
authors.lastname: Zhong
keywords: Cell proliferation; microRNAs; neoplasm metastasis; tongue cancer