title: Protection against influenza A virus by vaccination with a recombinant fusion protein linking influenza M2e to human serum albumin (HSA).
abstract: The highly conserved extracellular domain of M2 protein (M2e) of influenza A viruses has limited immunogenicity on its own. Hence, aiming to enhance immunogenicity of M2e protein, optimal approaches remain to be established. In this study, we created recombinant fusion protein vaccines by linking M2e consensus sequence of influenza A viruses with C-terminal domain of human serum albumin (HSA). Then HSA/M2e recombinant fusion protein was studied. Our results showed that HSA/M2e could induce strong anti-M2e specific humoral immune responses in the established mice model. Administration of HSA/M2e with Freund's adjuvant resulted in a higher number of IFN-γ-producing cells compared to HSA/M2e or M2e peptide emulsified in Freund's adjuvant. Furthermore, HSA/M2e was able to reduce viral load in the mice lungs and provide significant protection against lethal challenge with an H1N1 or an H3N2 virus compared to controls. In conclusion, this study has demonstrated a potential vaccine that could provide protection in preventing the threat of influenza outbreak because of rapid variation of the influenza virus.
authors.affiliation: Department of Central Laboratory, China-Japan Union Hospital, Jilin University, Changchun, China.
authors.forename: Xupeng
authors.initials: X
authors.lastname: Mu
authors.affiliation: Department of Urology, The First Hospital, Jilin University, Changchun, China.
authors.forename: Kebang
authors.initials: K
authors.lastname: Hu
authors.affiliation: Department of Infectious Disease, The Second Affiliated Hospital, China Medical University, Shenyang, China.
authors.forename: Mohan
authors.initials: M
authors.lastname: Shen
authors.affiliation: Department of Biological Engineering, College of Pharmacy, Jilin University, Changchun, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Kong
authors.affiliation: Department of Biological Engineering, College of Pharmacy, Jilin University, Changchun, China.
authors.forename: Changhao
authors.initials: C
authors.lastname: Fu
authors.affiliation: Department of Biological Engineering, College of Pharmacy, Jilin University, Changchun, China.
authors.forename: Weiqun
authors.initials: W
authors.lastname: Yan
authors.affiliation: Department of Biological Engineering, College of Pharmacy, Jilin University, Changchun, China. Electronic address: weian_hui@126.com.
authors.forename: Anhui
authors.initials: A
authors.lastname: Wei
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D056738:Cross Protection; D004196:Disease Outbreaks / Q000517:prevention & control; D005260:Female; D005620:Freund's Adjuvant / Q000276:immunology; D006801:Humans; D056724:Immunity, Humoral; D053118:Influenza A Virus, H1N1 Subtype / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D053122:Influenza A Virus, H3N2 Subtype / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D053124:Influenza A Virus, H5N1 Subtype / Q000276:immunology; D007252:Influenza Vaccines / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology*; D007251:Influenza, Human / Q000517:prevention & control*; D007371:Interferon-gamma / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009976:Orthomyxoviridae Infections / Q000276:immunology / Q000517:prevention & control / Q000821:virology; D011993:Recombinant Fusion Proteins / Q000008:administration & dosage / Q000276:immunology; D012709:Serum Albumin; D014611:Vaccination; D014614:Vaccines, Synthetic; D019562:Viral Load; D014763:Viral Matrix Proteins / Q000235:genetics / Q000276:immunology*; D000086082:Viroporin Proteins
chemical_list: D000914:Antibodies, Viral; D005620:Freund's Adjuvant; D007252:Influenza Vaccines; D007371:Interferon-gamma; D011993:Recombinant Fusion Proteins; D012709:Serum Albumin; D014614:Vaccines, Synthetic; D014763:Viral Matrix Proteins; C488937:M2 protein, Influenza A virus; D000086082:Viroporin Proteins
keywords: HSA; Immune protection; Influenza A virus; M2e; Vaccine