title: MiR-374a promotes the proliferation of osteosarcoma cell proliferation by targeting Axin2.
abstract: BACKGROUND
  MicroRNA-374a (miR-374a) has been implicated in several cancers. However, its role in osteosarcoma (OS) remains unclear. Thus the aim of this study was to investigate its expression and role in progression of OS.
  
  
  METHODS
  Quantitative real-time PCR (qRT-PCR) was performed to detect the expression of miR-374a in OS cell lines and tissues. To further understand its role, we restored expression of miR-374a in MG63 cell line by transfection with miR-374a mimics or inhibitors. Effects of miR-374a on cell proliferation on targets were also determined.
  
  
  RESULTS
  In the present study, our results showed that miR-374a was significantly up-regulated in both OS cell lines and OS tissues. Over expression of miR-374a markedly accelerated proliferation of OS cells, while its inhibition significantly suppressed cell proliferation. Moreover, Axin2 was identified to be a functional downstream target of miR-374a, and decreased expression of Axin2 could promote OS cell proliferation.
  
  
  CONCLUSIONS
  Our study suggested that miR-374a functions as an oncogene in OS, and the miR-374a/Axin2 axis might represent a potential therapeutic target for OS intervention.
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Xinxiang Medical University Weihui 453100, China.
authors.forename: Tan
authors.initials: T
authors.lastname: Lu
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Xinxiang Medical University Weihui 453100, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Xinxiang Medical University Weihui 453100, China.
authors.forename: Ming-Xiang
authors.initials: MX
authors.lastname: Chai
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Xinxiang Medical University Weihui 453100, China.
authors.forename: Yong-Bo
authors.initials: YB
authors.lastname: An
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Xinxiang Medical University Weihui 453100, China.
authors.forename: Jin-Ling
authors.initials: JL
authors.lastname: Jia
mesh_terms: D020413:3' Untranslated Regions; D060466:Axin Protein / Q000235:genetics / Q000378:metabolism*; D001665:Binding Sites; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D001859:Bone Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D012516:Osteosarcoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D034622:RNA Interference; D015398:Signal Transduction; D013997:Time Factors; D014162:Transfection
chemical_list: D020413:3' Untranslated Regions; C491761:AXIN2 protein, human; D060466:Axin Protein; D014408:Biomarkers, Tumor; C587995:MIRN374 microRNA 374, human; D035683:MicroRNAs
keywords: Axin2; Osteosarcoma; miR-374a; proliferation