title: Changes in wheat kernel proteins induced by microwave treatment.
abstract: Wheat kernels were subjected to microwave treatment, and the proteins were characterized by size exclusion high-performance liquid chromatography (SE-HPLC) and sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). Using this process, the proteins polymerize, forming intermolecular bonds among the same classes of proteins. Furthermore, the polymerization occurs only through disulphide bonds. Although SDS-PAGE did not show any differences for either the number or intensity of protein bands between flour samples before and after microwave treatment, gliadins from treated flours showed significantly reduced cross-reactivity with the R5 antibody. Moreover, the gluten became soluble in an aqueous saline solution, and it was not possible to isolate it using the Glutomatic apparatus. However, the treated flour, in the presence of water, was able to form dough and leaven and produce bread.
authors.affiliation: Dipartimento di Scienze Agrarie, degli Alimenti e dell'Ambiente, Università degli Studi di Foggia, Via Napoli, 25, 71122 Foggia, Italy. Electronic address: carmela.lamacchia@unifg.it.
authors.forename: Carmela
authors.initials: C
authors.lastname: Lamacchia
authors.affiliation: Dipartimento di Scienze Agrarie, degli Alimenti e dell'Ambiente, Università degli Studi di Foggia, Via Napoli, 25, 71122 Foggia, Italy.
authors.forename: Loretta
authors.initials: L
authors.lastname: Landriscina
authors.affiliation: Dipartimento di Scienze Agrarie, degli Alimenti e dell'Ambiente, Università degli Studi di Foggia, Via Napoli, 25, 71122 Foggia, Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: D'Agnello
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D005433:Flour / Q000032:analysis; D005511:Food Handling; D005903:Gliadin / Q000737:chemistry; D005983:Glutens / Q000737:chemistry; D008872:Microwaves; D010940:Plant Proteins / Q000737:chemistry*; D014908:Triticum / Q000737:chemistry*
chemical_list: D010940:Plant Proteins; D005983:Glutens; D005903:Gliadin
keywords: Gluten proteins; Microwave treatment; R5 antibody; SDS–PAGE; SE-HPLC