title: Transcriptomic Response to Nitric Oxide Treatment in Larix olgensis Henry.
abstract: Larix olgensis Henry is an important coniferous species found in plantation forests in northeastern China, but it is vulnerable to pathogens. Nitric oxide (NO) is an important molecule involved in plant resistance to pathogens. To study the regulatory role of NO at the transcriptional level, we characterized the transcriptomic response of L. olgensis seedlings to sodium nitroprusside (SNP, NO donor) using Illumina sequencing and de novo transcriptome assembly. A significant number of putative metabolic pathways and functions associated with the unique sequences were identified. Genes related to plant pathogen infection (FLS2, WRKY33, MAPKKK, and PR1) were upregulated with SNP treatment. This report describes the potential contribution of NO to disease resistance in L. olgensis as induced by biotic stress. Our results provide a substantial contribution to the genomic and transcriptomic resources for L. olgensis, as well as expanding our understanding of the involvement of NO in defense responses at the transcriptional level.
authors.affiliation: State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 26 Hexing Road, Harbin 150040, China. 15246344643@163.com.
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 26 Hexing Road, Harbin 150040, China. yifan85831647@sina.com.
authors.forename: Jingli
authors.initials: J
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 26 Hexing Road, Harbin 150040, China. chli0@163.com.
authors.forename: Chenghao
authors.initials: C
authors.lastname: Li
mesh_terms: D002473:Cell Wall / Q000378:metabolism; D016000:Cluster Analysis; D019295:Computational Biology / Q000379:methods; D060467:Disease Resistance / Q000235:genetics / Q000276:immunology; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D063990:Gene Ontology; D054884:Host-Pathogen Interactions; D028221:Larix / Q000187:drug effects / Q000235:genetics* / Q000276:immunology / Q000378:metabolism*; D058977:Molecular Sequence Annotation; D009569:Nitric Oxide / Q000378:metabolism* / Q000494:pharmacology; D009599:Nitroprusside / Q000494:pharmacology; D015203:Reproducibility of Results; D059467:Transcriptome
chemical_list: D009599:Nitroprusside; D009569:Nitric Oxide
keywords: Larix olgensis Henry; disease resistance; nitric oxide; transcriptome