title: Higher Urinary Levels of 8-Hydroxy-2'-deoxyguanosine Are Associated with a Worse RANKL/OPG Ratio in Postmenopausal Women with Osteopenia.
abstract: Postmenopausal osteoporosis (PO) is a major public health issue which affects a large fraction of elderly women. Emerging in vitro evidence suggests a central role of oxidative stress (OxS) in postmenopausal osteoporosis (PO) development. Contrariwise, the human studies on this topic are still scarce and inconclusive. In the attempt to address this issue, we sought to determine if OxS, as assessed by 8-hydroxy-2-deoxyguanosine (8-OHdG), may influence the level of receptor activator of nuclear factor-κb ligand (RANKL)/osteoprotegerin (OPG) ratio (a central regulator of bone metabolism) in a sample (n = 124), including postmenopausal women with osteoporosis, osteopenia and normal bone mass density (BMD). The most striking result that emerged in our study was the independent and positive (beta = 0.449, p = 0.004, and R(2) = 0.185) association between the OxS marker and RANKL/OPG ratio which was found in osteopenic but not in the other 2 sample groups. If confirmed by longitudinal studies, our findings would suggest that OxS is implicated in the derangement of bone homeostasis which precedes PO development. In line with these considerations, antioxidant treatment of postmenopausal women with moderately low BMD might contribute to preventing PO and related complications.
authors.affiliation: Department of Biomedical and Specialist Surgical Sciences, Section of Medical Biochemistry, Molecular Biology and Genetics, University of Ferrara, Via Borsari 46, 44121 Ferrara, Italy.
authors.forename: Carlo
authors.initials: C
authors.lastname: Cervellati
authors.affiliation: Department of Biomedical and Specialist Surgical Sciences, Section of Medical Biochemistry, Molecular Biology and Genetics, University of Ferrara, Via Borsari 46, 44121 Ferrara, Italy.
authors.forename: Arianna
authors.initials: A
authors.lastname: Romani
authors.affiliation: Department of Nutrition, University of California, One Shields Avenue, Davis, CA 95616, USA.
authors.forename: Eleonora
authors.initials: E
authors.lastname: Cremonini
authors.affiliation: Department of Biomedical and Specialist Surgical Sciences, Section of Medical Biochemistry, Molecular Biology and Genetics, University of Ferrara, Via Borsari 46, 44121 Ferrara, Italy.
authors.forename: Carlo M
authors.initials: CM
authors.lastname: Bergamini
authors.affiliation: Department of Morphology, Surgery and Experimental Medicine, Menopause and Osteoporosis Centre, University of Ferrara, Via Boschetto 29, 44124 Ferrara, Italy ; Department of Morphology, Surgery and Experimental Medicine, Section of Obstetrics and Gynecology, University of Ferrara, Via Aldo Moro 8, Cona, 44124 Ferrara, Italy.
authors.forename: Enrica
authors.initials: E
authors.lastname: Fila
authors.affiliation: Department of Biomedical and Specialist Surgical Sciences, Section of Medical Biochemistry, Molecular Biology and Genetics, University of Ferrara, Via Borsari 46, 44121 Ferrara, Italy.
authors.forename: Monica
authors.initials: M
authors.lastname: Squerzanti
authors.affiliation: Department of Morphology, Surgery and Experimental Medicine, Section of Obstetrics and Gynecology, University of Ferrara, Via Aldo Moro 8, Cona, 44124 Ferrara, Italy.
authors.forename: Pantaleo
authors.initials: P
authors.lastname: Greco
authors.affiliation: Department of Morphology, Surgery and Experimental Medicine, Menopause and Osteoporosis Centre, University of Ferrara, Via Boschetto 29, 44124 Ferrara, Italy ; Department of Morphology, Surgery and Experimental Medicine, Section of Orthopaedic Clinic, University of Ferrara, Via Aldo Moro 8, Cona, 44124 Ferrara, Italy.
authors.forename: Leo
authors.initials: L
authors.lastname: Massari
authors.affiliation: Department of Morphology, Surgery and Experimental Medicine, Menopause and Osteoporosis Centre, University of Ferrara, Via Boschetto 29, 44124 Ferrara, Italy ; Department of Morphology, Surgery and Experimental Medicine, Section of Obstetrics and Gynecology, University of Ferrara, Via Aldo Moro 8, Cona, 44124 Ferrara, Italy.
authors.forename: Gloria
authors.initials: G
authors.lastname: Bonaccorsi
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D015519:Bone Density; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000652:urine; D005260:Female; D006801:Humans; D008875:Middle Aged; D015663:Osteoporosis, Postmenopausal / Q000473:pathology / Q000652:urine*; D053244:Osteoprotegerin / Q000378:metabolism*; D053245:RANK Ligand / Q000378:metabolism*
chemical_list: D053244:Osteoprotegerin; D053245:RANK Ligand; C506030:TNFRSF11B protein, human; C505688:TNFSF11 protein, human; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine