title: Inhibition of quorum sensing, biofilm, and spoilage potential in Shewanella baltica by green tea polyphenols.
abstract: We investigated the quorum sensing (QS) system of Shewanella baltica and the anti-QS related activities of green tea polyphenols (TP) against spoilage bacteria in refrigerated large yellow croaker. Autoinducer-2 (AI-2) and the diketopiperazines (DKPs) cyclo-(L-Pro-L-Leu) and cyclo-(L-Pro-L-Phe) were detected in the culture extract of S. baltica XH2, however, no N-acylhomoserine lactones (AHLs) activity was observed. Green TP at sub-inhibitory concentrations interfered with AI-2 and DKPs activities of S. baltica without inhibiting cell growth and promoted degradation of AI-2. The green TP treatment inhibited biofilm development, exopolysaccharide production and swimming motility of S. baltica in a concentration- dependent manner. In addition, green TP decreased extracellular protease activities and trimethylamine production in S. baltica. A transcriptional analysis showed that green TP repressed the luxS and torA genes in S. baltica, which agreed with the observed reductions in QS activity and the spoilage phenotype. Epigallocatechin gallate (EGCG)-enriched in green TP significantly inhibited AI-2 activity of S. baltica. These findings strongly suggest that green TP could be developed as a new QS inhibitor for seafood preservation to enhance shelf life.
authors.affiliation: College of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou, 310018, P. R. China. junlizhu0305@163.com.
authors.forename: Junli
authors.initials: J
authors.lastname: Zhu
authors.affiliation: College of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou, 310018, P. R. China.
authors.forename: Xuzheng
authors.initials: X
authors.lastname: Huang
authors.affiliation: College of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou, 310018, P. R. China.
authors.forename: Fang
authors.initials: F
authors.lastname: Zhang
authors.affiliation: College of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou, 310018, P. R. China.
authors.forename: Lifang
authors.initials: L
authors.lastname: Feng
authors.affiliation: College of Chemistry, Chemical Engineering and Food Safety, Bohai University, Food Safety Key Lab of Liaoning Province, Jinzhou, 121013, P. R. China.
authors.forename: Jianrong
authors.initials: J
authors.lastname: Li
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D018441:Biofilms / Q000187:drug effects*; D028241:Camellia sinensis / Q000737:chemistry*; D013437:Carbon-Sulfur Lyases / Q000235:genetics; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D054659:Diketopiperazines / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005519:Food Preservation / Q000379:methods*; D005798:Genes, Bacterial / Q000187:drug effects; D006714:Homoserine / Q000031:analogs & derivatives / Q000378:metabolism; D007783:Lactones / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D010089:Oxidoreductases, N-Demethylating / Q000235:genetics; D010473:Perciformes / Q000382:microbiology; D059808:Polyphenols / Q000494:pharmacology*; D053038:Quorum Sensing / Q000187:drug effects*; D017747:Seafood / Q000382:microbiology*; D020592:Shewanella / Q000187:drug effects* / Q000235:genetics / Q000502:physiology
chemical_list: D001426:Bacterial Proteins; D054659:Diketopiperazines; D007783:Lactones; C091088:N-octanoylhomoserine lactone; D059808:Polyphenols; D006714:Homoserine; D002392:Catechin; C045651:epigallocatechin gallate; D010089:Oxidoreductases, N-Demethylating; C023186:trimethylamine dehydrogenase; D013437:Carbon-Sulfur Lyases; C117753:LuxS protein, Bacteria
keywords: AI-2; Shewanella baltica; biofilm; diketopiperazines; fish spoilage; green tea polyphenols