title: Dibutyl Maleate and Dibutyl Fumarate Enhance Contact Sensitization to Fluorescein Isothiocyanate in Mice.
abstract: Di-n-butyl phthalate (DBP), a phthalate ester, has been shown to have an adjuvant effect on fluorescein isothiocyanate (FITC)-induced contact hypersensitivity (CHS) mouse models. Di-n-butyl maleate (DBM), widely used as a plasticizer for industrial application, has been reported to cause dermatitis in humans. DBM is a butyl alcohol ester of di-carboxylic acid that represents a part of the DBP structure, while di-n-butyl fumarate (DBF) is a trans isomer of DBM. We examined whether DBM or DBF exhibits an adjuvant effect like DBP does. When BALB/c mice were epicutaneously sensitized with FITC in the presence of DBM or DBF, the FITC-specific CHS response was enhanced, as we have observed for DBP. As to underlying mechanisms, DBM and DBF facilitated the trafficking of FITC-presenting CD11c(+) dendritic cells (DCs) from skin to draining lymph nodes and increased the cytokine production by draining lymph nodes. In conclusion, DBM and DBF may have an effect that aggravates contact dermatitis through a skin sensitization process.
authors.affiliation: Laboratory of Microbiology and Immunology, School of Pharmaceutical Sciences, University of Shizuoka.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Matsuoka
authors.forename: Kohta
authors.initials: K
authors.lastname: Kurohane
authors.forename: Wakana
authors.initials: W
authors.lastname: Suzuki
authors.forename: Erina
authors.initials: E
authors.lastname: Ogawa
authors.forename: Kamiyu
authors.initials: K
authors.lastname: Kobayashi
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Imai
mesh_terms: D000818:Animals; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D003877:Dermatitis, Contact; D004342:Drug Hypersensitivity; D016650:Fluorescein-5-isothiocyanate / Q000633:toxicity*; D005650:Fumarates / Q000633:toxicity*; D005786:Gene Expression Regulation / Q000187:drug effects / Q000276:immunology; D008198:Lymph Nodes / Q000378:metabolism; D008298:Maleates / Q000633:toxicity*; D051379:Mice; D008807:Mice, Inbred BALB C; D015394:Molecular Structure
chemical_list: D016207:Cytokines; D005650:Fumarates; D008298:Maleates; C048074:dibutyl maleate; C000611872:dibutyl fumarate; D016650:Fluorescein-5-isothiocyanate