title: [Isolation and Study on the Aflatoxin Genes of Aflatoxin-producing Fungi in Paprika Samples in Chengdu].
abstract: OBJECTIVE
  To isolate aflatoxin-producing strains from paprika samples and to do a preliminarily study on the relationship between aflatoxin-producing ability and the genes aflR, omt-1 and ver-1.
  
  
  METHODS
  Fungi were isolated by traditional culture method. Potential aflatoxin-producing strains were screened by phenotypic traits and multiplex PCR. After these potential aflatoxin-producing strains cultured in the toxigenic culture medium, the levels of aflatoxin B, (AFB1) of the cultures were tested with ELISA method. The phylogenetic tree of aflR, omt-1 and ver-1 was constructed to explore the relationship between these genes and the AFB1-producing capacity.
  
  
  RESULTS
  17 potential aflatoxin-producing fungi were isolated. The ratio of positive toxigenic strains is 64. 71%. 11 isolates were positive in AFB1 detection while existing high sequence homology with AS 3. 4408, 6 isolates were negative in AFB1 detection while existing high sequence homology with Aspergillus oryzae.
  
  
  CONCLUSIONS
  Aspergillus flavus are potential candidates for aflatoxin control. Not all Aspergillus flavus have AFB1-producing capacity, aflR gene had a direct relation to AFB1-producing capacity, while ver-1 and omt-1 were related to the level of AFB1 producing.
authors.forename: Li
authors.initials: L
authors.lastname: Ling
authors.forename: Zheng-mao
authors.initials: ZM
authors.lastname: Ye
authors.forename: Su-yuan
authors.initials: SY
authors.lastname: Wang
authors.forename: Yan
authors.initials: Y
authors.lastname: Jin
authors.forename: Yu-hang
authors.initials: YH
authors.lastname: Chen
authors.forename: Tian-li
authors.initials: TL
authors.lastname: Zheng
authors.forename: Li-shi
authors.initials: LS
authors.lastname: Zhang
authors.forename: Xiao-fang
authors.initials: XF
authors.lastname: Pei
mesh_terms: D000348:Aflatoxins / Q000235:genetics*; D001231:Aspergillus flavus / Q000235:genetics*; D002212:Capsicum / Q000382:microbiology*; D005800:Genes, Fungal; D010802:Phylogeny
chemical_list: D000348:Aflatoxins