title: Improvement in physical and biological properties of chitosan/soy protein films by surface grafted heparin.
abstract: A series of chitosan/soy protein isolate (SPI) composite films (CS-n, n=0, 10 and 30, corresponding to SPI content in the composites) were prepared. Heparin was grafted onto the surface of CS-n to fabricate a series of heparinized films (HCS-n). CS-n and HCS-n were characterized by ATR-Fourier transform infrared spectroscopy and water contact angle. The surface heparin density was measured by toluidine blue assay. The results showed that heparin has been successfully grafted onto the surface of CS-n. Heparin evenly distributed on the surface of the films and the heparin content increased with the increase of SPI content, and the hydrophilicity of the films was enhanced due to the grafted heparin. The cytocompatibility and hemocompatibility of CS-n and HCS-n were evaluated by cell culture (MTT assay, live/dead assay, cell morphology and cell density observation), platelet adhesion test, plasma recalcification time (PRT) measurement, hemolysis assay and thrombus formation test. HCS-n showed higher cell adhesion rate and improved cytocompatibility compared to the corresponding CS-n. HCS-n also exhibited lower platelet adhesion, longer PRT, higher blood anticoagulant indexes (BCI) and lower hemolysis rate than the corresponding CS-n. The improved cytocompatibility and hemocompatibility of HCS-n would shed light on the potential applications of chitosan/soy protein-based biomaterials that may come into contact with blood.
authors.affiliation: Department of Biomedical Engineering, School of Basic Medical Sciences, Wuhan University, Wuhan 430071, China; Department of Biological Science and Technology, Wuhan Bioengineering Institute, Wuhan 430415, China.
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Wang
authors.affiliation: Anesthesiology Department, Hubei Tianyou Hospital Affiliated to Wuhan University of Science & Technology, Wuhan 430064, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Hu
authors.affiliation: Department of Biomedical Engineering, School of Basic Medical Sciences, Wuhan University, Wuhan 430071, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Biomedical Engineering, School of Basic Medical Sciences, Wuhan University, Wuhan 430071, China.
authors.forename: Li
authors.initials: L
authors.lastname: Gan
authors.affiliation: Jiangsu Provincial Key Laboratory of Eco-Environmental Materials, Yancheng Institute of Technology, Yancheng, Jiangsu 224051, China.
authors.forename: Meng
authors.initials: M
authors.lastname: He
authors.affiliation: Department of Biomedical Engineering, School of Basic Medical Sciences, Wuhan University, Wuhan 430071, China.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: He
authors.affiliation: Department of Biomedical Engineering, School of Basic Medical Sciences, Wuhan University, Wuhan 430071, China.
authors.forename: Weiqun
authors.initials: W
authors.lastname: Tian
authors.affiliation: Department of Biomedical Engineering, School of Basic Medical Sciences, Wuhan University, Wuhan 430071, China.
authors.forename: Mingming
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Biomedical Engineering, School of Basic Medical Sciences, Wuhan University, Wuhan 430071, China.
authors.forename: Li
authors.initials: L
authors.lastname: Xu
authors.affiliation: Department of Biomedical Engineering, School of Basic Medical Sciences, Wuhan University, Wuhan 430071, China. Electronic address: liyinping@whu.edu.cn.
authors.forename: Yinping
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Biomedical Engineering, School of Basic Medical Sciences, Wuhan University, Wuhan 430071, China. Electronic address: yunchen@whu.edu.cn.
authors.forename: Yun
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000818:Animals; D000925:Anticoagulants / Q000737:chemistry / Q000494:pharmacology; D001777:Blood Coagulation / Q000187:drug effects; D002460:Cell Line; D048271:Chitosan / Q000737:chemistry* / Q000494:pharmacology*; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D006461:Hemolysis / Q000187:drug effects; D006493:Heparin / Q000737:chemistry* / Q000494:pharmacology*; D057927:Hydrophobic and Hydrophilic Interactions; D008422:Materials Testing / Q000379:methods; D051379:Mice; D010973:Platelet Adhesiveness / Q000187:drug effects; D030262:Soybean Proteins / Q000737:chemistry* / Q000494:pharmacology*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D013499:Surface Properties; D013927:Thrombosis / Q000378:metabolism
chemical_list: D000925:Anticoagulants; D020099:Coated Materials, Biocompatible; D030262:Soybean Proteins; D006493:Heparin; D048271:Chitosan
keywords: Chitosan; Soy protein isolate; Surface heparinization