title: Avertoxins A-D, Prenyl Asteltoxin Derivatives from Aspergillus versicolor Y10, an Endophytic Fungus of Huperzia serrata.
abstract: Aspergillus versicolor Y10 is an endophytic fungus isolated from Huperzia serrata, which showed inhibitory activity against acetylcholinesterase. An investigation of the chemical constituents of Y10 led to the isolation of four new prenylated asteltoxin derivatives, named avertoxins A-D (2-5), together with the known mycotoxin asteltoxin (1). In the present study, we report structure elucidation for 2-5 and the revised NMR assignments for asteltoxin and demonstrated that avertoxin B (3) is an active inhibitor against human acetylcholinesterase with the IC50 value of 14.9 μM (huperzine A as the positive control had an IC50 of 0.6 μM). In addition, the cytotoxicity of asteltoxin (1) and avertoxins A-D (2-5) against MDA-MB-231, HCT116, and HeLa cell lines was evaluated.
authors.affiliation: Engineering Research Center of Industrial Microbiology (Ministry of Education), College of Life Sciences, Fujian Normal University , Fuzhou, Fujian 350117, People's Republic of China.
authors.forename: Mingzi
authors.initials: M
authors.lastname: Wang
authors.affiliation: Key Laboratory of Chemical Biology (Ministry of Education), School of Pharmaceutical Sciences, Shandong University , Jinan, Shandong 250012, People's Republic of China.
authors.forename: Mingwei
authors.initials: M
authors.lastname: Sun
authors.affiliation: Key Laboratory of Chemical Biology (Ministry of Education), School of Pharmaceutical Sciences, Shandong University , Jinan, Shandong 250012, People's Republic of China.
authors.forename: Huilin
authors.initials: H
authors.lastname: Hao
authors.affiliation: Key Laboratory of Chemical Biology (Ministry of Education), School of Pharmaceutical Sciences, Shandong University , Jinan, Shandong 250012, People's Republic of China.
authors.forename: Chunhua
authors.initials: C
authors.lastname: Lu
mesh_terms: D000470:Alkaloids; D001230:Aspergillus / Q000737:chemistry*; D002800:Cholinesterase Inhibitors / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D045325:HCT116 Cells; D006367:HeLa Cells; D006801:Humans; D032501:Huperzia / Q000382:microbiology*; D020128:Inhibitory Concentration 50; D015394:Molecular Structure; D009387:Neoprene; D019906:Nuclear Magnetic Resonance, Biomolecular; D011753:Pyrones / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D012717:Sesquiterpenes
chemical_list: D000470:Alkaloids; D002800:Cholinesterase Inhibitors; D011753:Pyrones; D012717:Sesquiterpenes; C016077:prenyl; C050426:huperzine A; C030923:asteltoxin; D009387:Neoprene