title: Fish collagen-based scaffold containing PLGA microspheres for controlled growth factor delivery in skin tissue engineering.
abstract: To design a scaffold controlled release system for skin tissue engineering, fish collagen/chitosan/chondroitin sulfate scaffolds were fabricated by freeze-drying and incorporated with bFGF-loaded PLGA microspheres (MPs). SEM showed that the scaffolds exhibited an interconnected porous structure, and the spherical MPs were uniformly distributed into the scaffolds. The higher swelling and degradation rate of scaffolds/MPs could lead to a higher diffusion rate of MPs from the scaffolds, causing an increase in the protein release. The release rate of proteins could be adjusted by the size of MPs and the ratio of collagen to chitosan of scaffolds. Circular dichroism spectroscopy and MTT of bFGF after release indicated that the released bFGF retained its structural integrity and bioactivity during preparation. Cell proliferation and in vivo evaluation results suggested that the scaffolds/MPs had a good biocompatibility and an ability to promote fibroblast cell proliferation and skin tissue regeneration. These results demonstrated that this scaffold/MP controlled release system has the potential for skin tissue engineering.
authors.affiliation: Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou 350002, China.
authors.forename: Huan
authors.initials: H
authors.lastname: Cao
authors.affiliation: Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou 350002, China. Electronic address: ibptcmm@fzu.edu.cn.
authors.forename: Ming-Mao
authors.initials: MM
authors.lastname: Chen
authors.affiliation: State Key Lab of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou 350002, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou 350002, China.
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Liu
authors.affiliation: Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou 350002, China.
authors.forename: Yu-Qing
authors.initials: YQ
authors.lastname: Huang
authors.affiliation: Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou 350002, China.
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Wang
authors.affiliation: Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou 350002, China.
authors.forename: Jing-Di
authors.initials: JD
authors.lastname: Chen
authors.affiliation: Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou 350002, China; Key Laboratory of Biomedical Material of Tianjin, Institute of Biomedical Engineering, Chinese Academy of Medical Science & Peking Union Medical College, Tianjin 300192, China. Electronic address: zhangqiq@126.com.
authors.forename: Qi-Qing
authors.initials: QQ
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002347:Carps; D003094:Collagen; D036341:Intercellular Signaling Peptides and Proteins / Q000008:administration & dosage*; D019344:Lactic Acid / Q000008:administration & dosage*; D008863:Microspheres; D011100:Polyglycolic Acid / Q000008:administration & dosage*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D051381:Rats; D012867:Skin / Q000254:growth & development*; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D003094:Collagen
keywords: Controlled release; Fish collagen; Growth factor; PLGA microspheres; Scaffold