title: Characterization of an Injury Induced Population of Muscle-Derived Stem Cell-Like Cells.
abstract: We recently discovered a novel population of stem cells from the injured murine skeletal muscle. These injury induced muscle-derived stem cell-like cells (iMuSCs) are partially reprogrammed from differentiated myogenic cells and display a pluripotent-like state. The iMuSCs exhibit stem cell properties including the ability to differentiate into multiple lineages, such as neurogenic and myogenic differentiations; they also display a superior migration capacity that demonstrating a strong ability of muscle engraftment in vivo. IMuSCs express several pluripotent and myogenic stem cell markers; have the capability to form embryoid bodies and teratomas, and can differentiate into all three germ layers. Moreover, blastocyst microinjection showed that the iMuSCs contributed to chimeric embryos but could not complete germline transmission. Our results indicate that the iMuSCs are in a partially reprogrammed state of pluripotency, which are generated by the microenvironment of injured skeletal muscle.
authors.affiliation: Department of Pediatric Surgery, University of Texas Medical School at Houston, TX 77030, USA.|Center for Stem Cell and Regenerative Medicine, University of Texas Health Science Center at Houston (UTHealth), TX 77030, USA.
authors.forename: Kinga
authors.initials: K
authors.lastname: Vojnits
authors.affiliation: Department of Pediatric Surgery, University of Texas Medical School at Houston, TX 77030, USA.|Center for Stem Cell and Regenerative Medicine, University of Texas Health Science Center at Houston (UTHealth), TX 77030, USA.
authors.forename: HaiYing
authors.initials: H
authors.lastname: Pan
authors.affiliation: Stem Cell Research Center, University of Pittsburgh, Medical School, Pittsburgh, PA 15213, USA.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Mu
authors.affiliation: Department of Pediatric Surgery, University of Texas Medical School at Houston, TX 77030, USA.|Center for Stem Cell and Regenerative Medicine, University of Texas Health Science Center at Houston (UTHealth), TX 77030, USA.
authors.forename: Yong
authors.initials: Y
authors.lastname: Li
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D001755:Blastocyst / Q000166:cytology / Q000378:metabolism; D002454:Cell Differentiation; D002465:Cell Movement; D065150:Cellular Reprogramming; D004622:Embryo, Mammalian; D058732:Embryoid Bodies / Q000166:cytology / Q000378:metabolism; D015870:Gene Expression; D005855:Germ Layers / Q000166:cytology / Q000378:metabolism; D016256:Lewis X Antigen / Q000235:genetics / Q000378:metabolism; D051957:MSX1 Transcription Factor / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008845:Microinjections; D018482:Muscle, Skeletal / Q000166:cytology* / Q000293:injuries / Q000378:metabolism; D032448:Myoblasts, Skeletal / Q000166:cytology* / Q000378:metabolism; D050814:Octamer Transcription Factor-3 / Q000235:genetics / Q000378:metabolism; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D019718:Receptors, CXCR4 / Q000235:genetics / Q000378:metabolism; D055748:SOXB1 Transcription Factors / Q000235:genetics / Q000378:metabolism; D013724:Teratoma / Q000378:metabolism / Q000473:pathology
chemical_list: D015415:Biomarkers; C514936:CXCR4 protein, mouse; D016256:Lewis X Antigen; D051957:MSX1 Transcription Factor; C484084:Msx1 protein, mouse; D050814:Octamer Transcription Factor-3; C494174:Pou5f1 protein, mouse; D019718:Receptors, CXCR4; D055748:SOXB1 Transcription Factors; C527783:Sox2 protein, mouse