title: Chemokine Receptors CXCR3 and CCR6 and Their Ligands in the Liver and Blood of Patients with Chronic Hepatitis C.
abstract: We performed a comprehensive analysis of CCR6 and CXCR3 chemokine receptors and their ligands CCL20/MIP-3α, CXCL9/MIG, CXCL10/IP-10, and CXCL11/ITAC in the liver and blood of patients with chronic hepatitis C at different stages of the disease. TaqMan PCR was used to determine mRNA gene expression of chemokines and their receptors in liver specimens, xMAP multiplex analysis was performed to estimate the concentration of chemokines in blood plasma, and fl ow cytofluorometry was used to evaluate CCR6 and CXCR3 expression on peripheral blood lymphocyte populations. In the liver of patients with hepatitis C, mRNA expression of CXCL10, CCR6, and CXCR3 genes increases with fibrosis progression in the liver tissue. In the plasma, concentrations of all studied chemokines increased depending on the stage of liver fibrosis, CCR6 and CXCR3 expression was changed in various lymphocyte populations. Thus, chemokines are involved in the immunopathogenesis and fibrogenesis in chronic viral hepatitis C. The results suggest using these chemokines in the diagnosis and prognosis of the disease.
authors.affiliation: Pasteur St. Petersburg Research Institute of Epidemiology and Microbiology, St. Petersburg, Russia. arsentieva_n.a@bk.ru.
authors.forename: N A
authors.initials: NA
authors.lastname: Arsent'eva
authors.affiliation: Pasteur St. Petersburg Research Institute of Epidemiology and Microbiology, St. Petersburg, Russia.|St. Petersburg I. P. Pavlov First State Medical University, St. Petersburg, Russia.
authors.forename: A V
authors.initials: AV
authors.lastname: Semenov
authors.affiliation: Pasteur St. Petersburg Research Institute of Epidemiology and Microbiology, St. Petersburg, Russia.
authors.forename: N E
authors.initials: NE
authors.lastname: Lyubimova
authors.affiliation: Pasteur St. Petersburg Research Institute of Epidemiology and Microbiology, St. Petersburg, Russia.
authors.forename: Yu V
authors.initials: YV
authors.lastname: Ostankov
authors.affiliation: Pasteur St. Petersburg Research Institute of Epidemiology and Microbiology, St. Petersburg, Russia.
authors.forename: D S
authors.initials: DS
authors.lastname: Elezo
authors.affiliation: Institute of Experimental Medicine, North-Western Division of the Russian Academy of Medical Sciences, St. Petersburg, Russia.
authors.forename: I V
authors.initials: IV
authors.lastname: Kudryavtsev
authors.affiliation: St. Petersburg State Pediatric Medical University, Ministry of Health of the Russian Federation, St. Petersburg, Russia.
authors.forename: V V
authors.initials: VV
authors.lastname: Basina
authors.affiliation: St. Petersburg State Pediatric Medical University, Ministry of Health of the Russian Federation, St. Petersburg, Russia.
authors.forename: E V
authors.initials: EV
authors.lastname: Esaulenko
authors.affiliation: S. M. Kirov Military Medical Academy, St. Petersburg, Russia.
authors.forename: K V
authors.initials: KV
authors.lastname: Kozlov
authors.affiliation: S. M. Kirov Military Medical Academy, St. Petersburg, Russia.
authors.forename: K V
authors.initials: KV
authors.lastname: Zhdanov
authors.affiliation: Pasteur St. Petersburg Research Institute of Epidemiology and Microbiology, St. Petersburg, Russia.|St. Petersburg I. P. Pavlov First State Medical University, St. Petersburg, Russia.
authors.forename: A A
authors.initials: AA
authors.lastname: Totolyan
mesh_terms: D018450:Disease Progression; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D019698:Hepatitis C, Chronic / Q000097:blood / Q000175:diagnosis* / Q000473:pathology; D006801:Humans; D008024:Ligands; D008103:Liver Cirrhosis / Q000097:blood / Q000175:diagnosis / Q000209:etiology; D008214:Lymphocytes / Q000276:immunology; D008297:Male; D054399:Receptors, CCR6 / Q000097:blood* / Q000378:metabolism*; D054367:Receptors, CXCR3 / Q000097:blood* / Q000378:metabolism*; D019707:Receptors, Chemokine / Q000097:blood / Q000235:genetics / Q000378:metabolism
chemical_list: C106311:CCR6 protein, human; C515187:CXCR3 protein, human; D008024:Ligands; D054399:Receptors, CCR6; D054367:Receptors, CXCR3; D019707:Receptors, Chemokine
keywords: biomarkers; chemokines; fibrogenesis; immunopathogenesis; viral hepatitis C