title: Generation of Induced Pluripotent Stem Cells (iPSCs) from Adult Canine Fibroblasts.
abstract: Induced pluripotent stem cells hold great potential in regenerative medicine as it enables to generate pluripotent stem cells from any available cell types. Ectopic expression of four transcription factors (Oct4, Sox2, Klf4, and c-Myc) can reprogram fibroblasts directly to pluripotency as shown in multiple species. Here, we describe detailed protocols for generation of iPSCs from adult canine fibroblasts. Robust canine iPSCs will provide powerful tools not only to study human diseases, but also for the development of therapeutic approaches.
authors.affiliation: Department of Cell Biology, Duke University, Durham, NC, 27710, USA.|Duke University Medical Center, Duke University, Durham, NC, 27710, USA.
authors.forename: Sehwon
authors.initials: S
authors.lastname: Koh
authors.affiliation: Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, 1060 William Moore Drive, Raleigh, NC, 27607, USA. jorge_piedrahita@ncsu.edu.|Genomics Program, North Carolina State University, Raleigh, NC, 27607, USA. jorge_piedrahita@ncsu.edu.|Center for Comparative Medicine and Translational Research, North Carolina State University, Raleigh, NC, 27607, USA. jorge_piedrahita@ncsu.edu.
authors.forename: Jorge A
authors.initials: JA
authors.lastname: Piedrahita
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D065150:Cellular Reprogramming; D004285:Dogs; D061252:Feeder Cells; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism*; D005822:Genetic Vectors / Q000235:genetics; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism*; D000090062:Kruppel-Like Factor 4; D051379:Mice; D012190:Retroviridae / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics; D014161:Transduction, Genetic; D019076:Transgenes
chemical_list: C000715467:KLF4 protein, human; C000715468:Klf4 protein, mouse; D000090062:Kruppel-Like Factor 4; D014157:Transcription Factors
keywords: Canine; Induced pluripotent stem cells; iPS cells