title: Wogonoside induces apoptosis in Bel-7402, a hepatocellular carcinoma cell line, by regulating Bax/Bcl-2.
abstract: The anticancer effect ofScutellaria baicalensisextract has recently become a topic of interest. In this study, the anticancer effects and underlying mechanisms of wogonoside, the main constituent ofScutellaria baicalensis, were investigated in a human hepatocellular carcinoma (HCC) cell linein vitro.The effects of wogonoside on the proliferation, cell cycle progression and apoptosis of hepatocellular carcinoma cells were examined. Western blotting was employed to analyze the proteins associated with the biological effects of wogonoside. Wogonoside exerted anti-proliferation propertiesin vitro. HCC cell growth was attenuated by wogonoside (8 µM) treatment. Cell cycle progression analysis and DNA ladder assay revealed that apoptosis was enhanced in wogonoside-treated cells and that cell cycle arrest occurred in the G2/M phase. It was also demonstrated that increased apoptosis was accompanied by increased levels of Bax protein and decreased levels of Bcl-2 protein. The results of this study suggest that wogonoside may represent a potential therapeutic agent against HCC.
authors.affiliation: Department of Orthopaedics, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.
authors.forename: Yusheng
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Orthopaedics, Second People's Hospital of Jingmen, Jingmen, Hubei 448000, P.R. China.
authors.forename: Min
authors.initials: M
authors.lastname: Tu
authors.affiliation: Department of Orthopaedics, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.
authors.forename: Chao
authors.initials: C
authors.lastname: Cheng
authors.affiliation: Department of Orthopaedics, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.
authors.forename: Jian
authors.initials: J
authors.lastname: Tian
authors.affiliation: Department of Orthopaedics, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.
authors.forename: Fangjie
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Department of Orthopaedics, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.
authors.forename: Zhenhan
authors.initials: Z
authors.lastname: Deng
authors.affiliation: Department of Orthopaedics, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.
authors.forename: Xuan'an
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Cell Biology, School of Life Sciences, Central South University, Changsha, Hunan 410008, P.R. China.
authors.forename: Zhongkui
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Cell Biology, School of Life Sciences, Central South University, Changsha, Hunan 410008, P.R. China.
authors.forename: Yanping
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Orthopaedics, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.
authors.forename: Guanghua
authors.initials: G
authors.lastname: Lei
keywords: Bax; apoptosis; hepatocellular carcinoma; wogonoside