title: MicroRNA-299-3p promotes the sensibility of lung cancer to doxorubicin through directly targeting ABCE1.
abstract: MicroRNAs (miRNAs) are a class of endogenous, small non-coding RNAs which play important roles in various biological and cellular processes, including chemoresistance. The expression level of miR-299-3p was dysregulated in doxorubicin-resistance lung cancer cell lines. However, the exact role of miR-299-3p in doxorubicin-resistance is still unknown. In the present study, miR-299-3p was down-expressed in doxorubicin-resistant or -sensitive lung cancer samples and it was identified to directly targeted adenosine triphosphate binding cassette E1 (ABCE1) 3'-untranslated region (UTR) in lung cancer H69 cells by luciferase assay. After transfection of miR-299-3p mimics or ABCE1-siRNA, MTT assay confirmed that the H69/ADR cell proliferation was inhibited, as well as the enhanced cell inhibitory rate in the presence of doxorubicin. H69/ADR cell apoptosis rate was promoted after miR-299-3p or ABCE1-siRNA transfection. The results indicated that miR-299-3p promotes the sensibility of lung cancer to doxorubicin through suppression of ABCE1, at least partly. Therefore, the disordered decreased of miR-299-3p and resulting ABCE1 up-expression may contribute to chemoresistance of lung cancer, and miR-299-3p-ABCE1 may represent a new potential therapeutic target for the treatment of chemoresistance of lung cancer.
authors.affiliation: Department of Respiratory, Nanyang City Center Hospital Nanyang 473009, Henan, PR China.
authors.forename: Dawei
authors.initials: D
authors.lastname: Zheng
authors.affiliation: Department of Respiratory, Nanyang City Center Hospital Nanyang 473009, Henan, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Dai
authors.affiliation: Department of Endocrinology, Nanyang City Center Hospital Nanyang 473009, Henan, PR China.
authors.forename: Song
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Cardiovascular Surgery, Nanyang City Center Hospital Nanyang 473009, Henan, PR China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Xing
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism*; D000328:Adult; D000368:Aged; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics*; D049109:Cell Proliferation / Q000187:drug effects / Q000235:genetics*; D004317:Doxorubicin / Q000494:pharmacology* / Q000627:therapeutic use; D019008:Drug Resistance, Neoplasm / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged
chemical_list: C501917:ABCE1 protein, human; D018528:ATP-Binding Cassette Transporters; D035683:MicroRNAs; D004317:Doxorubicin
keywords: Lung cancer; adenosine triphosphate binding cassette E1; chemoresistance; miR-299-3p