title: Identification and characterization of natural antibodies against tau protein in an intravenous immunoglobulin product.
abstract: The latest therapeutic approaches to Alzheimer disease are using intravenous immunoglobulin (IVIG) products. Therefore, the detailed characterization of target-specific antibodies naturally occurring in IVIG products is beneficial. We have focused on characterization of antibodies isolated against tau protein, a biomarker of Alzheimer's disease, from Flebogamma IVIG product. The analysis of IgG subclass distribution indicated skewing toward IgG3 in anti-tau-enriched IgG fraction. The evaluation of their reactivity and avidity with several recombinant tau forms was performed by ELISA and blotting techniques. Truncated non-phosphorylated tau protein (amino acids 155-421) demonstrated the highest reactivity and avidity index. We provide the first detailed insight into the reactivity of isolated natural antibodies against tau protein.
authors.affiliation: Department of Neurobiology, AD Center, National Institute of Mental Health, Klecany, Czech Republic; Faculty of Science, Charles University in Prague, Prague, Czech Republic; Department of Biological and Biochemical Sciences, University of Pardubice, Pardubice, Czech Republic.
authors.forename: Lenka
authors.initials: L
authors.lastname: Hromadkova
authors.affiliation: Department of Neurobiology, AD Center, National Institute of Mental Health, Klecany, Czech Republic; Third Faculty of Medicine, Charles University in Prague, Prague, Czech Republic. Electronic address: michala.kolarova@nudz.cz.
authors.forename: Michala
authors.initials: M
authors.lastname: Kolarova
authors.affiliation: Department of Biological and Biochemical Sciences, University of Pardubice, Pardubice, Czech Republic.
authors.forename: Barbora
authors.initials: B
authors.lastname: Jankovicova
authors.affiliation: Department of Neurobiology, AD Center, National Institute of Mental Health, Klecany, Czech Republic; Third Faculty of Medicine, Charles University in Prague, Prague, Czech Republic.
authors.forename: Ales
authors.initials: A
authors.lastname: Bartos
authors.affiliation: Department of Neurobiology, AD Center, National Institute of Mental Health, Klecany, Czech Republic.
authors.forename: Jan
authors.initials: J
authors.lastname: Ricny
authors.affiliation: Department of Biological and Biochemical Sciences, University of Pardubice, Pardubice, Czech Republic.
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Bilkova
authors.affiliation: Department of Neurobiology, AD Center, National Institute of Mental Health, Klecany, Czech Republic.
authors.forename: Daniela
authors.initials: D
authors.lastname: Ripova
mesh_terms: D000939:Epitopes / Q000737:chemistry; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology / Q000302:isolation & purification*; D016756:Immunoglobulins, Intravenous / Q000737:chemistry* / Q000276:immunology; D007163:Immunosorbent Techniques; D008970:Molecular Weight; D010455:Peptides / Q000276:immunology; D011485:Protein Binding / Q000276:immunology; D017434:Protein Structure, Tertiary; D016875:tau Proteins / Q000276:immunology*
chemical_list: D000939:Epitopes; D007074:Immunoglobulin G; D016756:Immunoglobulins, Intravenous; D010455:Peptides; D016875:tau Proteins
keywords: Alzheimer disease; Avidity; Immunoreactivity; Intravenous immunoglobulins; Natural antibodies; Tau protein