title: Aberrant gene expression of heparanase in ventricular hypertrophy induced by monocrotaline in rats.
abstract: The gene expression levels of heparanase, matrix metalloproteinase 2 (MMP2) and MMP9 were examined in ventricles after treatment with monocrotaline (MCT) to induce cardiac hypertrophy in rats. Rats received a single intraperitoneal injection of MCT (60 mg/kg) or saline. Twenty-five days after the injection, the right ventricle and lung wet weights were increased in MCT-treated rats compared with the control. Histological analysis revealed cardiomyocyte hypertrophy in the right ventricle of MCT-treated rats. Northern blot hybridization showed that heparanase and MMP2 expression increased significantly in the right and left ventricles of MCT-treated rats, whereas MMP9 was not induced. These findings indicate that heparanase and MMP2 might play an important role in the development of MCT-induced cardiac hypertrophy.
authors.affiliation: Department of Biological Resources, Integrated Center for Sciences, Ehime University, Shitsukawa, Toon, Ehime 791-0295, Japan.
authors.forename: Toshina
authors.initials: T
authors.lastname: Ishiguro-Oonuma
authors.forename: Masako
authors.initials: M
authors.lastname: Suemoto
authors.forename: Muneyoshi
authors.initials: M
authors.lastname: Okada
authors.forename: Kazuki
authors.initials: K
authors.lastname: Yoshioka
authors.forename: Yukio
authors.initials: Y
authors.lastname: Hara
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Hashizume
authors.forename: Keiichiro
authors.initials: K
authors.lastname: Kizaki
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D015870:Gene Expression; D005966:Glucuronidase / Q000235:genetics*; D006352:Heart Ventricles / Q000378:metabolism; D017380:Hypertrophy, Right Ventricular / Q000139:chemically induced / Q000201:enzymology*; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000235:genetics; D020780:Matrix Metalloproteinase 9 / Q000235:genetics; D016686:Monocrotaline; D051381:Rats; D017208:Rats, Wistar; D000099175:Heparanase
chemical_list: D005966:Glucuronidase; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; D016686:Monocrotaline; D000099175:Heparanase; C522363:Mmp2 protein, rat; C572337:Mmp9 protein, rat