title: Microarray Analysis of Gene Expression at the Tumor Front of Colon Cancer.
abstract: Budding or the presence poorly differentiated clusters at the boundary of cancer tissue is a pathologically important finding and serves as a prognostic factor in colorectal cancer. However, few studies have examined the cancer tissue boundary in clinical samples. The purpose of the present study was to examine gene expression at the tumor front of colon cancer in surgically resected samples. Cancer tissues were obtained by laser microdissection of 20 surgically resected specimens. Genes with significantly different microarray signals between the tumor front and the tumor center were identified. Among genes showing significant up-regulation at the tumor front were six chemokines [chemokine c-c motif ligand (CCL)2 and -18, chemokine (C-X-C motif) ligand (CXCL)9-11, and interleukin 8 (IL8)], and two apoptosis-related molecules [ubiquitin D (UBD) and baculoviral iap repeat-containing 3 (BIRC3)]. Expression of laminin gamma 2 (LAMC2), matrix metallopeptidase 7 (MMP7) and epithelial-mesenchymal transition (EMT)-related molecules were elevated in the tumor front, but their fold changes were smaller than those of the aforementioned genes. These results suggest that chemokines, in addition to EMT-related molecules, may play important roles in invasion of colon cancer.
authors.affiliation: Department of Surgery, Kyorin University Hospital, Mitaka, Tokyo, Japan ck9t-kbys@asahi-net.or.jp.
authors.forename: Takaaki
authors.initials: T
authors.lastname: Kobayashi
authors.affiliation: Department of Surgery, Kyorin University Hospital, Mitaka, Tokyo, Japan.
authors.forename: Tadahiko
authors.initials: T
authors.lastname: Masaki
authors.affiliation: Department of Surgery, Kyorin University Hospital, Mitaka, Tokyo, Japan.
authors.forename: Eriko
authors.initials: E
authors.lastname: Nozaki
authors.affiliation: Department of Surgery, Kyorin University Hospital, Mitaka, Tokyo, Japan.
authors.forename: Masanori
authors.initials: M
authors.lastname: Sugiyama
authors.affiliation: Department of Medical Oncology, Kyorin University Hospital, Mitaka, Tokyo, Japan.
authors.forename: Fumio
authors.initials: F
authors.lastname: Nagashima
authors.affiliation: Department of Medical Oncology, Kyorin University Hospital, Mitaka, Tokyo, Japan.
authors.forename: Junji
authors.initials: J
authors.lastname: Furuse
authors.affiliation: Department of Laboratory Medicine, Kyorin University Hospital, Mitaka, Tokyo, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Onishi
authors.affiliation: Department of Laboratory Medicine, Kyorin University Hospital, Mitaka, Tokyo, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Watanabe
authors.affiliation: Department of Pathology, Kyorin University Hospital, Mitaka, Tokyo, Japan.
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Ohkura
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D003110:Colonic Neoplasms / Q000235:genetics* / Q000473:pathology; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics*; D005260:Female; D015870:Gene Expression; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D008297:Male; D046228:Microarray Analysis / Q000379:methods*; D008875:Middle Aged
keywords: Gene-expression profiling; chemokines; colon cancer; epithelial–mesenchymal transition; microarray; tumor front