title: Stereotaxic Surgical Targeting of the Nonhuman Primate Caudate and Putamen: Gene Therapy for Huntington's Disease.
abstract: Stereotaxic surgery is an invaluable tool to deliver a variety of gene therapy constructs to the nonhuman primate caudate and putamen in preclinical studies for the genetic, neurodegenerative disorder, Huntington's disease (HD). Here we describe in detail how to perform this technique beginning with a pre-surgical magnetic resonance imaging scan to determine surgical coordinates followed by the stereotaxic surgical injection technique. In addition, we include methodology of a full necropsy including brain and peripheral tissue removal and a standard immunohistochemical technique to visualize the injected gene therapy agent.
authors.affiliation: Division of Neuroscience, Oregon National Primate Research Center, Beaverton, OR, USA. mcbridej@ohsu.edu.|Department of Behavioral Neuroscience, Oregon Health and Science University, Portland, OR, USA. mcbridej@ohsu.edu.|Department of Neurology, Oregon Health and Science University, Portland, OR, USA. mcbridej@ohsu.edu.
authors.forename: Jodi L
authors.initials: JL
authors.lastname: McBride
authors.affiliation: Division of Neuroscience, Oregon National Primate Research Center, Beaverton, OR, USA.
authors.forename: Randall L
authors.initials: RL
authors.lastname: Clark
mesh_terms: D000818:Animals; D002421:Caudate Nucleus / Q000378:metabolism*; D004195:Disease Models, Animal; D015316:Genetic Therapy; D006801:Humans; D000071058:Huntingtin Protein; D006816:Huntington Disease / Q000235:genetics / Q000473:pathology / Q000628:therapy*; D008253:Macaca mulatta; D008279:Magnetic Resonance Imaging; D009419:Nerve Tissue Proteins / Q000235:genetics*; D011699:Putamen / Q000378:metabolism*; D013238:Stereotaxic Techniques
chemical_list: C086055:HTT protein, human; D000071058:Huntingtin Protein; D009419:Nerve Tissue Proteins
keywords: Huntington’s disease; Immunohistochemistry; Magnetic resonance imaging; Necropsy; Neurodegeneration; Nonhuman primate; Stereotaxic surgery