title: Characterization and function analysis of Hsp60 and Hsp10 under different acute stresses in black tiger shrimp, Penaeus monodon.
abstract: Heat shock proteins (Hsps) are a class of highly conserved proteins produced in virtually all living organisms from bacteria to humans. Hsp60 and Hsp10, the most important mitochondrial chaperones, participate in environmental stress responses. In this study, the full-length complementary DNAs (cDNAs) of Hsp60 (PmHsp60) and Hsp10 (PmHsp10) were cloned from Penaeus monodon. Sequence analysis showed that PmHsp60 and PmHsp10 encoded polypeptides of 578 and 102 amino acids, respectively. The expression profiles of PmHsp60 and PmHsp10 were detected in the gills and hepatopancreas of the shrimps under pH challenge, osmotic stress, and heavy metal exposure, and results suggested that PmHsp60 and PmHsp10 were involved in the responses to these stimuli. ATPase and chaperone activity assay indicated that PmHsp60 could slow down protein denaturation and that Hsp60/Hsp10 may be combined to produce a chaperone complex with effective chaperone and ATPase activities. Overall, this study provides useful information to help further understand the functional mechanisms of the environmental stress responses of Hsp60 and Hsp10 in shrimp.
authors.affiliation: South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, 510300, China.|College of Aqua-life Science and Technology, Shanghai Ocean University, Shanghai, 201306, China.
authors.forename: Jinxuan
authors.initials: J
authors.lastname: Shi
authors.affiliation: South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, 510300, China.|Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture, Guangzhou, 510300, China.
authors.forename: Mingjun
authors.initials: M
authors.lastname: Fu
authors.affiliation: South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, 510300, China.|Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture, Guangzhou, 510300, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Zhao
authors.affiliation: South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, 510300, China.|Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture, Guangzhou, 510300, China.
authors.forename: Falin
authors.initials: F
authors.lastname: Zhou
authors.affiliation: South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, 510300, China.|Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture, Guangzhou, 510300, China.|Tropical Aquaculture Research and Development Center of South China Sea Fisheries Research Institute, Sanya, 572000, China.
authors.forename: Qibin
authors.initials: Q
authors.lastname: Yang
authors.affiliation: South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, 510300, China. qiu902@yahoo.com.|Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture, Guangzhou, 510300, China. qiu902@yahoo.com.
authors.forename: Lihua
authors.initials: L
authors.lastname: Qiu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D060829:Arthropod Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D018835:Chaperonin 10 / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D018834:Chaperonin 60 / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D005880:Gills / Q000737:chemistry / Q000502:physiology; D043143:Hepatopancreas / Q000737:chemistry / Q000502:physiology; D006863:Hydrogen-Ion Concentration; D019216:Metals, Heavy / Q000378:metabolism; D009997:Osmotic Pressure; D033561:Penaeidae / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D010802:Phylogeny; D013312:Stress, Physiological
chemical_list: D060829:Arthropod Proteins; D018835:Chaperonin 10; D018834:Chaperonin 60; D019216:Metals, Heavy
keywords: Heavy metal exposure; Hsp10; Hsp60; Osmotic stress; Penaeus monodon; pH challenge