title: Pluronic F127 as a drug vehicle used in chick embryo chorioallantoic membrane shell-less model.
abstract: The developing vascular network is grown on the surface of chick embryo chorioallantoic membrane (CAM), so CAM is widely used as an in vivo model to study the angiogenesis. Because the CAM is hindered or wrinkled by the vehicle, the drug effect is difficult to be observed. In the present study, we firstly introduced the pluronic F127 aquogel to deliver drugs for the CAM model. The biocompatibility and advantage of this vehicle was shown by applied ranibizumab-pluronic F127 mix on the CAM. The results were showed that, the growth of blood vessels was not impaired by pluronic F127 gel, and the gel was almost imperceptible on the CAM, at the same time, the degradation of blood capillaries caused by ranibizumab was clearly visible. In conclusion, pluronic F127 was a good vehicle for angiogenesis research.
authors.affiliation: Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan, Shanxi, PR China.
authors.forename: Tonglin
authors.initials: T
authors.lastname: Shi
authors.affiliation: Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan, Shanxi, PR China.
authors.forename: Zongwei
authors.initials: Z
authors.lastname: Li
authors.affiliation: Central Laboratory of Taiyuan Central Hospital, No. 1, East Sandao Alley, Xinghualing District, Taiyuan, Shanxi, PR China.
authors.forename: Quanbin
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Cornea Department of Shanxi Eye Hospital, No. 100, Fudong Street, Taiyuan, Shanxi, PR China.
authors.forename: Dawen
authors.initials: D
authors.lastname: Zheng
authors.affiliation: Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan, Shanxi, PR China.
authors.forename: Zhuoyu
authors.initials: Z
authors.lastname: Li
mesh_terms: D020533:Angiogenesis Inhibitors / Q000008:administration & dosage*; D000818:Animals; D002642:Chick Embryo; D049033:Chorioallantoic Membrane / Q000098:blood supply*; D004337:Drug Carriers; D020100:Hydrogels; D023421:Models, Animal; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D020442:Poloxamer / Q000737:chemistry*; D000069579:Ranibizumab / Q000008:administration & dosage*
chemical_list: D020533:Angiogenesis Inhibitors; D004337:Drug Carriers; D020100:Hydrogels; D020442:Poloxamer; D000069579:Ranibizumab