title: Deferrisoma palaeochoriense sp. nov., a thermophilic, iron(III)-reducing bacterium from a shallow-water hydrothermal vent in the Mediterranean Sea.
abstract: A novel thermophilic, anaerobic, mixotrophic bacterium, designated strain MAG-PB1T, was isolated from a shallow-water hydrothermal vent system in Palaeochori Bay off the coast of the island of Milos, Greece. The cells were Gram-negative, rugose, short rods, approximately 1.0 μm long and 0.5 μm wide. Strain MAG-PB1T grew at 30-70 °C (optimum 60 °C), 0-50 g NaCl l- 1 (optimum 15-20 g l- 1) and pH 5.5-8.0 (optimum pH 6.0). Generation time under optimal conditions was 2.5 h. Optimal growth occurred under chemolithoautotrophic conditions with H2 as the energy source and CO2 as the carbon source. Fe(III), Mn(IV), arsenate and selenate were used as electron acceptors. Peptone, tryptone, Casamino acids, sucrose, yeast extract, d-fructose, α-d-glucose and ( - )-d-arabinose also served as electron donors. No growth occurred in the presence of lactate or formate. The G+C content of the genomic DNA was 66.7 mol%. Phylogenetic analysis of the 16S rRNA gene sequence indicated that this organism is closely related to Deferrisoma camini, the first species of a recently described genus in the Deltaproteobacteria. Based on the 16S rRNA gene phylogenetic analysis and on physiological, biochemical and structural characteristics, the strain was found to represent a novel species, for which the name Deferrisoma palaeochoriense sp. nov. is proposed. The type strain is MAG-PB1T ( = JCM 30394T = DSM 29363T).
authors.affiliation: Geophysical Laboratory, Carnegie Institution of Washington, Washington DC, 20015, USA.
authors.forename: Ileana
authors.initials: I
authors.lastname: Pérez-Rodríguez
authors.affiliation: Geophysical Laboratory, Carnegie Institution of Washington, Washington DC, 20015, USA.|Department of Oceanic, Atmospheric and Earth Sciences, George Mason University, Fairfax, VA 22030, USA.
authors.forename: Matthew
authors.initials: M
authors.lastname: Rawls
authors.affiliation: US Geological Survey, National Fish Health Research Laboratory, Leetown Science Center, Kearneysville, WV 25430, USA.
authors.forename: D Katharine
authors.initials: DK
authors.lastname: Coykendall
authors.affiliation: Geophysical Laboratory, Carnegie Institution of Washington, Washington DC, 20015, USA.
authors.forename: Dionysis I
authors.initials: DI
authors.lastname: Foustoukos
mesh_terms: D001421:Bacteria, Anaerobic / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D020564:Deltaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D005227:Fatty Acids / Q000737:chemistry; D006115:Greece; D060086:Hydrothermal Vents / Q000382:microbiology*; D007501:Iron / Q000378:metabolism; D008522:Mediterranean Sea; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D007501:Iron