title: Metabolite Signatures in Hydrophilic Extracts of Mouse Lungs Exposed to Cigarette Smoke Revealed by1H NMR Metabolomics Investigation.
abstract: 1H-NMR metabolomics was used to investigate the changes of metabolites in the lungs of mice with and without being exposed to a controlled amount of cigarette smoke. It was found that the concentrations of adenosine derivatives (i.e. ATP, ADP and AMP), inosine and uridine were significantly changed in the lungs of mice exposed to cigarette smoke when compared with controls regardless the mice were obese or of regular weight. The decreased ATP, ADP, AMP and elevated inosine suggested that the deaminases in charge of adenosine derivatives to inosine derivatives conversion would be significantly changed in the lungs of mice exposed to cigarette smoke. Indeed, transcriptional study confirmed that the concentrations of adenosine monophosphate deaminase 2 and adenosine deaminase 2 were significantly changed in the lungs of mice exposed to cigarette smoke. We also found that the ratio of glycerophosphocholine (GPC) to phosphocholine (PC) was significantly increased in the lungs of obese mice compared with those of the regular weight mice. The GPC/PC ratio was further elevated in the lungs of obese group exposed to cigarette smoke.
authors.affiliation: Pacific Northwest National Laboratory, Richland, WA, USA.
authors.forename: Hu
authors.initials: H
authors.lastname: Jz
authors.affiliation: Pacific Northwest National Laboratory, Richland, WA, USA ; State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Institute of Physics and Mathematics, the Chinese Academy of Sciences, Wuhan, China.
authors.forename: Wang
authors.initials: W
authors.lastname: X
authors.affiliation: Pacific Northwest National Laboratory, Richland, WA, USA.
authors.forename: Feng
authors.initials: F
authors.lastname: J
authors.affiliation: Pacific Northwest National Laboratory, Richland, WA, USA.
authors.forename: Robertson
authors.initials: R
authors.lastname: Bj
authors.affiliation: Pacific Northwest National Laboratory, Richland, WA, USA.
authors.forename: Waters
authors.initials: W
authors.lastname: Km
authors.affiliation: Pacific Northwest National Laboratory, Richland, WA, USA.
authors.forename: Tilton
authors.initials: T
authors.lastname: Sc
authors.affiliation: Pacific Northwest National Laboratory, Richland, WA, USA.
authors.forename: Pounds
authors.initials: P
authors.lastname: Jg
authors.affiliation: Pacific Northwest National Laboratory, Richland, WA, USA.
authors.forename: Corley
authors.initials: C
authors.lastname: Ra
authors.affiliation: State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Institute of Physics and Mathematics, the Chinese Academy of Sciences, Wuhan, China.
authors.forename: Liu
authors.initials: L
authors.lastname: M
authors.affiliation: Pacific Northwest National Laboratory, Richland, WA, USA.
authors.forename: Hu
authors.initials: H
authors.lastname: M
keywords: 1H-NMR metabolomics; Cigarette smoke; Mouse lungs; OPLS; Obesity; PCA