title: Enzymatic generation of galactose-rich oligosaccharides/oligomers from potato rhamnogalacturonan I pectic polysaccharides.
abstract: Potato pulp by-product rich in galactan-rich rhamnogalacturonan I (RG I) was investigated as a new source of oligosaccharides with potential prebiotic properties. The efficiency of selected monocomponent enzymes and multi-enzymatic preparations to generate oligosaccharides/oligomers from potato RG I was evaluated. These overall results of yield were dependent on the activity profile of the multi-enzymatic preparations. Highest oligo-RG I yield of 93.9% was achieved using multi-enzymatic preparation (Depol 670L) with higher hydrolytic activity toward side chains of RG I as compared to its backbone. Main oligo-RG I products were oligosaccharides with DP of 2-12 (79.8-100%), while the oligomers with DP of 13-70 comprised smaller proportion (0.0-20.2%). Galactose (58.9-91.2%, w/w) was the main monosaccharide of oligo-RG I, while arabinose represented 0.0-12.1%. An understanding of the relationship between the activity profile of multi-enzymatic preparations and the yield/DP of oligo-RG I was achieved. This is expected to provide the capability to generate galacto- and galacto(arabino) oligosaccharides and their corresponding oligomers from an abundant by-product.
authors.affiliation: Department of Food Science and Agricultural Chemistry, McGill University, 21,111 Lakeshore, Ste-Anne de Bellevue, Quebec H9X 3V9, Canada.
authors.forename: Nastaran
authors.initials: N
authors.lastname: Khodaei
authors.affiliation: Department of Food Science and Agricultural Chemistry, McGill University, 21,111 Lakeshore, Ste-Anne de Bellevue, Quebec H9X 3V9, Canada. Electronic address: salwa.karboune@mcgill.ca.
authors.forename: Salwa
authors.initials: S
authors.lastname: Karboune
mesh_terms: D001089:Arabinose / Q000737:chemistry; D005685:Galactans / Q000737:chemistry; D005690:Galactose / Q000737:chemistry*; D006868:Hydrolysis; D009844:Oligosaccharides / Q000737:chemistry*; D010368:Pectins / Q000737:chemistry*; D056692:Prebiotics; D011198:Solanum tuberosum / Q000737:chemistry*
chemical_list: D005685:Galactans; D009844:Oligosaccharides; D056692:Prebiotics; C042491:rhamnogalacturonan I; D010368:Pectins; D001089:Arabinose; D005690:Galactose
keywords: Galacto-oligosaccharides; Multi-enzymatic preparations; Oligomers; Potato; Prebiotic; Rhamnogalacturan I