title: ANXA5 level is linked to in vitro and in vivo tumor malignancy and lymphatic metastasis of murine hepatocarcinoma cell.
abstract: OBJECTIVE
  To investigate ANXA5 overexpression on in vitro and in vivo malignancies of murine Hca-P cells.
  
  
  METHODS
  Hca-P with low lymph node metastasis (LNM) potential was used as cell model. TEM, CCK-8 and Boyden transwell assays were performed for in vitro Hca-P behaviors. Hca-P-transplanted mouse model was established for in vivo experiment.
  
  
  RESULTS
  ANXA5-overexpressing monoclonal Anxa5-Hca-P-1, Anxa5-Hca-P-2 and Anxa5-Hca-P-3 cells were obtained. ANXA5 upregulation alters the proliferation, morphology and rough endoplasmic reticulum of Hca-P cells, enhances in vitro migration and invasions of Hca-P, promotes in vivo malignant degree and LNM rate of Anxa5-Hca-P-3-transplanted mice.
  
  
  CONCLUSIONS
  As a potential indicator for malignancy and lymphatic metastasis, ANXA5 overexpression increases in vitro migration and invasion of Hca-P cell, promotes in vivo malignancy, LNM rate and level of Hca-P-transplanted mice.
authors.affiliation: Department of Biotechnology, Dalian Medical University, 9 West Section, Lvshun Southern Road, Dalian 116044, China.
authors.forename: Boya
authors.initials: B
authors.lastname: Peng
authors.affiliation: Department of Biochemistry, Dalian Medical University, 9 West Section, Lvshun Southern Road, Dalian 116044, China.
authors.forename: Shuqing
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Biotechnology, Dalian Medical University, 9 West Section, Lvshun Southern Road, Dalian 116044, China.
authors.forename: Chunmei
authors.initials: C
authors.lastname: Guo
authors.affiliation: Department of Biotechnology, Dalian Medical University, 9 West Section, Lvshun Southern Road, Dalian 116044, China.
authors.forename: Xujuan
authors.initials: X
authors.lastname: Sun
authors.affiliation: Department of Biotechnology, Dalian Medical University, 9 West Section, Lvshun Southern Road, Dalian 116044, China.
authors.forename: Ming-Zhong
authors.initials: MZ
authors.lastname: Sun
mesh_terms: D000818:Animals; D017304:Annexin A5 / Q000096:biosynthesis* / Q000235:genetics; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D004195:Disease Models, Animal; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000473:pathology; D008207:Lymphatic Metastasis / Q000235:genetics* / Q000473:pathology; D051379:Mice; D009361:Neoplasm Invasiveness / Q000235:genetics / Q000473:pathology
chemical_list: D017304:Annexin A5
keywords: ANXA5; Hca-P; hepatocarcinoma; invasion; lymphatic metastasis; migration