title: Protective effects against and potential mechanisms underlying the effect of magnesium isoglycyrrhizinate in hypoxia-reoxygenation injury in rat liver cells.
abstract: We examined the protective effects of magnesium isoglycyrrhizinate (MgIG) on hypoxia-reoxygenation injury in rat liver cells. Rat liver cells in the logarithmic growth phase were divided into the hypoxia-reoxygenation injury model group and MgIG pretreatment group (0.01, 0.1, 1, 10, 100 mg/mL). After 24-h pretreatment, we detected the effects of MgIG on liver cell viability using the methyl thiazolyl tetrazolium (MTT) assay at 6-h hypoxia and 4-h reoxygenation. After 24-h pretreatment, liver cells were randomly divided into the hypoxia-reoxygenation injury model group and low-, moderate-, and high-MgIG-concentration groups (0.1, 1, 10 mg/mL, respectively), and hypoxia and reoxygenation were simulated for 6 and 4 h, respectively. Cell morphology was observed by light microscopy. Nuclear factor-kB gene expression was analyzed by quantitative reverse transcription-polymerase chain reaction. MTT results showed that MgIG (0.1, 1, 10 mg/mL) improved the A-value of anoxia-reoxygenation injury in liver cells (P < 0.01) compared with that of the model group. Cells did not survive when the MgIG concentration was 100 mg/mL. At an MgIG concentration lower than 0.01 mg/mL, the A-value of the MTT group was higher than that of the model group (P > 0.05). Nuclear factor-kB mRNA expression (0.597 ± 0.062, 0.248 ± 0.067, 0.141 ± 0.029) in the low-, moderate-, and high-concentration groups was lower than that in the model group (P < 0.01). MgIG reduced hypoxia-reoxygenation injury of liver cells, indicating that it improved hepatic cell activity, inhibited lipid peroxidation and inflammatory reactions, and decreased nuclear factor-kB mRNA expression.
authors.affiliation: Peking University Shenzhen Hospital, Shenzhen, Guangdong, China.
authors.forename: J
authors.initials: J
authors.lastname: Zheng
authors.affiliation: Nanchang University School of Medicine, Nanchang, Jiangxi, China.
authors.forename: G
authors.initials: G
authors.lastname: Wu
authors.affiliation: Peking University Shenzhen Hospital, Shenzhen, Guangdong, China.
authors.forename: G X
authors.initials: GX
authors.lastname: Hu
authors.affiliation: Peking University Shenzhen Hospital, Shenzhen, Guangdong, China.
authors.forename: Y Z
authors.initials: YZ
authors.lastname: Peng
authors.affiliation: Peking University Shenzhen Hospital, Shenzhen, Guangdong, China.
authors.forename: X J
authors.initials: XJ
authors.lastname: Xiong
mesh_terms: D000818:Animals; D015415:Biomarkers; D015687:Cell Hypoxia; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D015870:Gene Expression; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D008297:Male; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D020011:Protective Agents / Q000494:pharmacology; D051381:Rats; D015427:Reperfusion Injury / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D012503:Saponins / Q000378:metabolism* / Q000494:pharmacology; D014315:Triterpenes / Q000378:metabolism* / Q000494:pharmacology
chemical_list: C521270:18alpha,20beta-hydroxy-11-oxo-norolean-12-en-3beta-yl-2-O-beta-D-glucopyranurosyl-alpha-D-glucopyranosiduronate magnesium tetrahydrate; D015415:Biomarkers; D016328:NF-kappa B; D020011:Protective Agents; D012503:Saponins; D014315:Triterpenes