title: Description ofDomibacillus iocasaesp. nov., isolated from deep-sea sediment, and emended description of the genusDomibacillus.
abstract: In this study, we report a novel Gram-stain-positive bacterium, designated strain S6T, isolated from deep-sea sediment in Okinawa Trough. Growth of strain S6Toccurred at 10-45 °C (optimum, 35 °C), at pH 6.0-11.0 (optimum, 8.0) and with 0-8 % (w/v) NaCl (optimum, 3 %). Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain S6Tbelonged to the genusDomibacillus. The closest phylogenetic neighbours of strain S6TwereDomibacillus robiginosusWS 4628T( = DSM 25058T) (98.6 %16S rRNA gene sequence similarity),D. indicusSD111T( = DSM 28032T) (97.6 %),D. enclensisNIO-1016T( = DSM 25145T) (97.6 %), and 'D. tundrae' PAMC 80007 ( = DSM 29572) (97.6 %). Levels of similarity between strain S6Tand other close relatives were below 96 %. The levels of DNA-DNA relatedness between strain S6Tand its closest relatives in the genusDomibacilluswere well below 70 %. The genomic DNA G+C content of strain S6Twas 44.4 mol%. The major menaquinone was MK-6. The polar lipids of strain S6Tcontained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol mannosides, phosphatidylethanolamine and three unknown phospholipids. Strain S6Tharbouredmeso-diaminopimelic acid as the diagnostic diamino acid and an A1γ type of peptidoglycan. The major cell-wall sugars of strain S6Twere xylose and glucose. Phylogenetic, physiological, biochemical and morphological analyses suggest that this strain represents a novel species of the genusDomibacillus, for which the nameDomibacillus iocasaesp. nov. is proposed. The type strain is S6T( = DSM 29979T= CCTCC AB 2015183T). An emended description of the genusDomibacillusis also provided.
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, PR China.|Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, PR China.|University of Chinese Academy of Sciences, Beijing, PR China.
authors.forename: Qing-Lei
authors.initials: QL
authors.lastname: Sun
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, PR China.|Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Sun