title: FADS2 genotype regulates delta-6 desaturase activity and inflammation in human adipose tissue.
abstract: Obesity is associated with disturbed lipid metabolism and low-grade inflammation in tissues. The aim of this study was to investigate the association between FA metabolism and adipose tissue (AT) inflammation in the Kuopio Obesity Surgery study. We investigated the association of surgery-induced weight loss and FA desaturase (FADS)1/2 genotypes with serum and AT FA profile and with AT inflammation, measured as interleukin (IL)-1β and NFκB pathway gene expression, in order to find potential gene-environment interactions. We demonstrated an association between serum levels of saturated and polyunsaturated n-6 FAs, and estimated enzyme activities of FADS1/2 genes with IL-1β expression in AT both at baseline and at follow-up. Variation in the FADS1/2 genes associated with IL-1β and NFκB pathway gene expression in SAT after weight reduction, but not at baseline. In addition, the FA composition in subcutaneous and visceral fat correlated with serum FAs, and the associations between serum PUFAs and estimated D6D enzyme activity with AT inflammation were also replicated with corresponding AT FAs and AT inflammation. We conclude that the polymorphism in FADS1/2 genes associates with FA metabolism and AT inflammation, leading to an interaction between weight loss and FADS1/2 genes in the regulation of AT inflammation.
authors.affiliation: Institutes of Public Health and Clinical Nutrition University of Eastern Finland, Kuopio, Finland.
authors.forename: Maija
authors.initials: M
authors.lastname: Vaittinen
authors.affiliation: Institutes of Public Health and Clinical Nutrition University of Eastern Finland, Kuopio, Finland.
authors.forename: Paula
authors.initials: P
authors.lastname: Walle
authors.affiliation: Institutes of Public Health and Clinical Nutrition University of Eastern Finland, Kuopio, Finland.
authors.forename: Emmi
authors.initials: E
authors.lastname: Kuosmanen
authors.affiliation: Departments of Medicine University of Eastern Finland and Kuopio University Hospital, Kuopio, Finland.
authors.forename: Ville
authors.initials: V
authors.lastname: Männistö
authors.affiliation: Surgery, University of Eastern Finland and Kuopio University Hospital, Kuopio, Finland.
authors.forename: Pirjo
authors.initials: P
authors.lastname: Käkelä
authors.affiliation: Biomedicine, University of Eastern Finland, Kuopio, Finland.
authors.forename: Jyrki
authors.initials: J
authors.lastname: Ågren
authors.affiliation: Institutes of Public Health and Clinical Nutrition University of Eastern Finland, Kuopio, Finland Clinical Nutrition and Obesity Center, Kuopio University Hospital, Kuopio, Finland.
authors.forename: Ursula
authors.initials: U
authors.lastname: Schwab
authors.affiliation: Institutes of Public Health and Clinical Nutrition University of Eastern Finland, Kuopio, Finland Clinical Nutrition and Obesity Center, Kuopio University Hospital, Kuopio, Finland jussi.pihlajamaki@uef.fi.
authors.forename: Jussi
authors.initials: J
authors.lastname: Pihlajamäki
mesh_terms: D000273:Adipose Tissue / Q000201:enzymology / Q000378:metabolism / Q000473:pathology; D000090943:Delta-5 Fatty Acid Desaturase; D044943:Fatty Acid Desaturases / Q000097:blood / Q000235:genetics* / Q000378:metabolism*; D043371:Fatty Acids, Omega-6 / Q000097:blood / Q000378:metabolism; D005231:Fatty Acids, Unsaturated / Q000378:metabolism; D005260:Female; D059647:Gene-Environment Interaction; D056726:Genetic Association Studies; D006801:Humans; D007249:Inflammation / Q000201:enzymology / Q000378:metabolism / Q000473:pathology; D053583:Interleukin-1beta / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D016328:NF-kappa B / Q000096:biosynthesis; D009765:Obesity / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D010084:Oxidation-Reduction; D015434:Panniculitis / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D020641:Polymorphism, Single Nucleotide / Q000235:genetics
chemical_list: D000090943:Delta-5 Fatty Acid Desaturase; D043371:Fatty Acids, Omega-6; D005231:Fatty Acids, Unsaturated; D053583:Interleukin-1beta; D016328:NF-kappa B; D044943:Fatty Acid Desaturases; C000716847:FADS1 protein, human; C533241:FADS2 protein, human
keywords: fatty acid; fatty acid desaturase 1; fatty acid/desaturases; fatty acid/metabolism; gene expression; genetics; obesity