title: Three-dimensional shape of the Golgi apparatus in different cell types: serial section scanning electron microscopy of the osmium-impregnated Golgi apparatus.
abstract: Although many studies of the Golgi apparatus structure have been performed by light and electron microscopy, the full shape of the Golgi apparatus remained unclear due to the technical limitations of the previously applied microscopy techniques. In this study, we used serial section scanning electron microscopy (SEM) for the morphological study of the Golgi apparatus. This method is useful for three-dimensional (3D) reconstruction of cellular structures without requiring specialized instruments, unlike focused ion beam SEM (FIB-SEM) and serial block face SEM (SBF-SEM). Using the serial section SEM method developed by our laboratory, we investigate the 3D shape of the osmium-impregnated Golgi apparatus in rat epididymal cells, pancreatic acinar cells and gonadotropes. The combination of serial section SEM and a 3D reconstruction technique enabled us to elucidate the entire shape of the Golgi apparatus in these cells. The full shape of the Golgi apparatus in epididymal cells formed a basket-like structure with oval-shaped cisterns, while the Golgi apparatus in an acinar cell from the pancreas was composed of elongated ribbon-like structures that were connected to each other, making a coarse network. The overall image of the Golgi apparatus cisterns from a gonadotrope looked like a spherical cage. This study has clearly shown that entire 3D shape of the Golgi apparatus varies depending on the cell type and that the Golgi cisterns network appears as a single mass located in the large region of the cytoplasm.
authors.affiliation: Division of Microscopic Anatomy and Bio-imaging, Niigata University Graduate School of Medical and Dental Sciences, Niigata 951-8510, Japan Department of Microscopic Anatomy and Cell Biology, Asahikawa Medical University, Asahikawa 078-8510, Japan daisukek@asahikawa-med.ac.jp.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Koga
authors.affiliation: Division of Microscopic Anatomy and Bio-imaging, Niigata University Graduate School of Medical and Dental Sciences, Niigata 951-8510, Japan Division of Morphological Sciences, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima 890-8544, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kusumi
authors.affiliation: Division of Microscopic Anatomy and Bio-imaging, Niigata University Graduate School of Medical and Dental Sciences, Niigata 951-8510, Japan.
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Ushiki
mesh_terms: D061354:Acinar Cells / Q000648:ultrastructure; D000818:Animals; D004822:Epididymis / Q000166:cytology; D006056:Golgi Apparatus / Q000648:ultrastructure*; D052681:Gonadotrophs / Q000648:ultrastructure; D016591:Histocytological Preparation Techniques / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000379:methods; D008297:Male; D008855:Microscopy, Electron, Scanning / Q000379:methods*; D009992:Osmium / Q000737:chemistry; D010179:Pancreas / Q000166:cytology; D051381:Rats; D017208:Rats, Wistar
chemical_list: D009992:Osmium
keywords: 3D printer; 3D reconstruction; Golgi apparatus; backscattered electron; osmium impregnation method; serial section SEM