title: Nonomuraea thermotoleranssp. nov., a thermotolerant actinomycete isolated from mushroom compost.
abstract: A novel thermotolerant actinomycete, designated strain 3-33-9BT, was isolated from mushroom compost in Nanning, Guangxi province, China. The strain grew at 25-60 °C, pH 7.0-11.0 and with 0-2.0 % (w/v) NaCl. Strain 3-33-9BTformed extensively branched aerial mycelia and substrate mycelia. Spiral spore chains with one to three turns were composed of about 3-15 non-motile spores with a wrinkled surface. The organism containedmeso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan. Whole-cell hydrolysates contained madurose, galactose, glucose and ribose. The cellular fatty acid profile mainly consisted of iso-C16 : 0and C17 : 0. The quinone system was predominantly composed of MK-9(H4). The DNA G+C content of strain 3-33-9BTwas 72.5 mol%. The organism showed a combination of morphological and chemotaxonomic properties typical of members of the genusNonomuraea. Moreover, 16S rRNA gene sequence analysis also showed that the organism belonged to the genusNonomuraea; in the 16S rRNA gene tree of the genusNonomuraea, strain 3-33-9BTformed a distinct phyletic line and was most closely related toNonomuraea fastidiosaNBRC 14680T(97.3 % 16S rRNA gene sequence similarity). However, phenotypic characteristics of strain 3-33-9BTwere significantly different fromN. fastidiosaNBRC 14680T, and DNA-DNA hybridization showed little relatedness (31.6-35.2 %) between the two strains. Based on the phenotypic and phylogenetic data, strain 3-33-9BTrepresents a novel species of the genusNonomuraea, for which the nameNonomuraea thermotoleranssp. nov. is proposed. The type strain is 3-33-9BT( = ATCC BAA-2629T= CGMCC 4.7161T).
authors.affiliation: College of Life Science and Technology, Guangxi University, Nanning, PR China.|Institute of Applied Microbiology, College of Agriculture, Guangxi University, Nanning, PR China.
authors.forename: Hao
authors.initials: H
authors.lastname: Wu
authors.affiliation: Institute of Applied Microbiology, College of Agriculture, Guangxi University, Nanning, PR China.
authors.forename: Bin
authors.initials: B
authors.lastname: Liu