title: Dual Stimuli-Responsive Vesicular Nanospheres Fabricated by Lipopolymer Hybrids for Tumor-Targeted Photodynamic Therapy.
abstract: Smart delivery system of photosensitizer chlorin e6 (Ce6) has been developed for targeted photodynamic therapy (PDT). Simple self-assemblies of the mixtures comprising soybean lecithin derived phosphatidylcholine (PC), phosphatidylethanolamine-poly(L-histidine)40 (PE-p(His)40), and folic acid (FA) conjugated phosphatidylethanolamine-poly(N-isopropylacrylamide)40 (PE-p(NIPAM)40-FA) in different ratios yield smart nanospheres characterized by (i) stable and uniform particle size (∼100 nm), (ii) positive surface charge, (iii) high hydrophobic drug (Ce6) loading efficiency up to 45%, (iv) covalently linked targeting moiety, (v) low cytotoxicity, and (vi) smartness showing p(His) block oriented pH and p(NIPAM) oriented temperature responsiveness. The Ce6-encapsulated vesicular nanospheres (Ce6@VNS) were used to confirm the efficiency of cellular uptake, intracellular distribution, and phototoxicity against KB tumor cells compared to free Ce6 at different temperature and pH conditions. The Ce6@VNS system showed significant photodynamic therapeutic efficiency on KB cells than free Ce6. A receptor-mediated inhibition study proved the site-specific delivery of Ce6 in targeted tumor cells.
authors.affiliation: BK21 PLUS Center for Advanced Chemical Technology, Department Polymer Science and Engineering, Pusan National University , Busan 609-735, Republic of Korea.
authors.forename: Johnson V
authors.initials: JV
authors.lastname: John
authors.affiliation: Department of Gastroenterology, Pusan National University Hospital , Busan 602-739, Republic of Korea.
authors.forename: Chung-Wook
authors.initials: CW
authors.lastname: Chung
authors.affiliation: BK21 PLUS Center for Advanced Chemical Technology, Department Polymer Science and Engineering, Pusan National University , Busan 609-735, Republic of Korea.
authors.forename: Renjith P
authors.initials: RP
authors.lastname: Johnson
authors.affiliation: Biomedical Research Institute, Pusan National University Hospital , Busan 602-739, Republic of Korea.
authors.forename: Young-Il
authors.initials: YI
authors.lastname: Jeong
authors.affiliation: Department of Anesthesiology and Pain Medicine, College of Medicine, The Catholic University , Seoul 137-701, Republic of Korea.
authors.forename: Kyu-Don
authors.initials: KD
authors.lastname: Chung
authors.affiliation: Department of Gastroenterology, Pusan National University Hospital , Busan 602-739, Republic of Korea.
authors.forename: Dae Hwan
authors.initials: DH
authors.lastname: Kang
authors.affiliation: Department of Chemistry and Chemistry Institute for Functional Materials, Pusan National University , Busan 609-735, Republic of Korea.
authors.forename: Hongsuk
authors.initials: H
authors.lastname: Suh
authors.affiliation: Division of Chemistry and Biological Chemistry, Nanyang Technological University , 21 Nanyang Link 637371 Singapore.
authors.forename: Hongyu
authors.initials: H
authors.lastname: Chen
authors.affiliation: BK21 PLUS Center for Advanced Chemical Technology, Department Polymer Science and Engineering, Pusan National University , Busan 609-735, Republic of Korea.
authors.forename: Il
authors.initials: I
authors.lastname: Kim
mesh_terms: D000178:Acrylamides / Q000737:chemistry; D045744:Cell Line, Tumor; D002735:Chlorophyllides; D006639:Histidine / Q000737:chemistry; D006801:Humans; D007624:KB Cells; D054118:Nanospheres / Q000008:administration & dosage* / Q000737:chemistry*; D009369:Neoplasms / Q000188:drug therapy*; D010778:Photochemotherapy / Q000379:methods; D017319:Photosensitizing Agents / Q000737:chemistry; D011108:Polymers / Q000008:administration & dosage* / Q000737:chemistry*; D011166:Porphyrins / Q000737:chemistry
chemical_list: D000178:Acrylamides; D002735:Chlorophyllides; D017319:Photosensitizing Agents; D011108:Polymers; D011166:Porphyrins; D006639:Histidine; C062985:phytochlorin; C067295:N-isopropylacrylamide