title: Somatic Embryogenesis of Abies cephalonica Loud.
abstract: Greek fir (Abies cephalonica Loudon) belongs to the Mediterranean fir species and is widely distributed in the mountains of Central and Southern Greece. Considering a climatic scenario, infestation by pathogens or insects and fire episodes, it has been proposed that Mediterranean firs could be in danger in some parts of their present range but, on the other hand, could also replace other species in more northern zones with temperate humid climates (e.g., silver fir, Abies alba Mill.). As fir species are generally highly productive and therefore important for commercial forestry, they have traditionally been involved in conventional tree improvement programs. A lot of effort has been put into the development of vegetative propagation methods for firs, in order to rapidly gain the benefits of traditional breeding to be utilized in reforestation. The present paper provides up to date information on protocols for somatic embryogenesis (i.e., the most promising in vitro method for vegetative propagation) of Greek fir. Moreover, the protocols for cryopreservation and long-term storage of embryogenic material are described as well.
authors.affiliation: Department of Agriculture and Environmental Science, University of Udine, Via delle Scienze 91, Udine, 33100, Italy. jana.krajnakova@uniud.it.|Genetics and Physiology Department, University of Oulu, P.O. Box 3000, Oulu, 90014, Finland. jana.krajnakova@uniud.it.
authors.forename: Jana
authors.initials: J
authors.lastname: Krajňáková
authors.affiliation: Genetics and Physiology Department, University of Oulu, P.O. Box 3000, Oulu, 90014, Finland.
authors.forename: Hely
authors.initials: H
authors.lastname: Häggman
mesh_terms: D028202:Abies / Q000235:genetics / Q000254:growth & development*; D015925:Cryopreservation; D016468:Forestry; D006115:Greece; D063245:Plant Development / Q000235:genetics*; D058439:Plant Somatic Embryogenesis Techniques / Q000379:methods*; D012639:Seeds / Q000235:genetics / Q000254:growth & development; D046509:Tissue Culture Techniques / Q000379:methods*
keywords: Cryopreservation; Ectomycorrhizal fungi; Fulvic acid s; Greek fir; Initiation; Maturation; Proliferation