title: MicroRNA-650 in a copy number-variable region regulates the production of interleukin 6 in human osteosarcoma cells.
abstract: Copy number variation is a well-known genetic variation. microRNAs (miRNAs/miRs) are non-coding RNAs that mediate gene expression by regulating target mRNAs. In the present study, copy number deletions encompassing miRNA coding regions were investigated to determine the association between the deletion of miRNA and its phenotypic effects. A total of 38 human miRNAs in copy number variants were identified and miR-650, which is functional in the human osteosarcoma MG-63 cell line, was selected. Overexpression of miR-650 decreased the expression of inhibitor of growth family member 4 (ING4) in the MG-63 cells and increased interleukin (IL)6 transcription, as well as IL6 secretion in IL1B-stimulated cells. Furthermore, miR-650 downregulated the amount of nuclear factor of κ light polypeptide gene enhancer in B cells inhibitor α and increased the transcriptional activity of nuclear factor (NF)κB. Downregulation of ING4 also increased the production of IL6, similar to miR-650 overexpression. Taken together, these data indicate that miR-650 plays a significant role in the production of IL6 by regulating ING4 expression and NFκB signaling in IL1B-stimulated MG-63 osteosarcoma cells.
authors.affiliation: Center for Genome Science, Korea National Institute of Health, Cheongju, Chungcheongbuk-do 361-951, Republic of Korea ; College of Pharmacy, Chungbuk National University, Cheongju, Chungcheongbuk-do 361-763, Republic of Korea.
authors.forename: Jun Ho
authors.initials: JH
authors.lastname: Yun
authors.affiliation: Center for Genome Science, Korea National Institute of Health, Cheongju, Chungcheongbuk-do 361-951, Republic of Korea.
authors.forename: Sanghoon
authors.initials: S
authors.lastname: Moon
authors.affiliation: Center for Genome Science, Korea National Institute of Health, Cheongju, Chungcheongbuk-do 361-951, Republic of Korea.
authors.forename: Heun-Sik
authors.initials: HS
authors.lastname: Lee
authors.affiliation: Center for Genome Science, Korea National Institute of Health, Cheongju, Chungcheongbuk-do 361-951, Republic of Korea.
authors.forename: Mi Yeong
authors.initials: MY
authors.lastname: Hwang
authors.affiliation: Center for Genome Science, Korea National Institute of Health, Cheongju, Chungcheongbuk-do 361-951, Republic of Korea.
authors.forename: Yeon-Jung
authors.initials: YJ
authors.lastname: Kim
authors.affiliation: Center for Genome Science, Korea National Institute of Health, Cheongju, Chungcheongbuk-do 361-951, Republic of Korea.
authors.forename: Ho-Yeong
authors.initials: HY
authors.lastname: Yu
authors.affiliation: College of Pharmacy, Chungbuk National University, Cheongju, Chungcheongbuk-do 361-763, Republic of Korea.
authors.forename: Youngsoo
authors.initials: Y
authors.lastname: Kim
authors.affiliation: Center for Genome Science, Korea National Institute of Health, Cheongju, Chungcheongbuk-do 361-951, Republic of Korea.
authors.forename: Bok-Ghee
authors.initials: BG
authors.lastname: Han
authors.affiliation: Center for Genome Science, Korea National Institute of Health, Cheongju, Chungcheongbuk-do 361-951, Republic of Korea.
authors.forename: Bong-Jo
authors.initials: BJ
authors.lastname: Kim
authors.affiliation: Center for Genome Science, Korea National Institute of Health, Cheongju, Chungcheongbuk-do 361-951, Republic of Korea.
authors.forename: Jeong-Min
authors.initials: JM
authors.lastname: Kim
keywords: ING4; copy number variation; interleukin 6; microRNA-650