title: Proteasomes raise the microtubule dynamics in influenza A (H1N1) virus-infected LLC-MK2 cells.
abstract: The dynamics of microtubule networks are known to have an impact on replication of influenza A virus in some cellular models. Here we present evidence suggesting that at late stages of LLC-MK2 cell infection by influenza A (H1N1) virus the ubiquitin-proteasome protein degradation system participates in destabilization of microtubules, and favours virus replication. Chemical inhibition of proteasome activity partially suppresses influenza A virus replication, while stimulation of proteasome activity favours influenza A virus replication. Conversely, in another cellular model, A549 cells, inhibitors and activators of proteasomes have a small effect on influenza A virus replication. These data suggest that influenza A virus might take selective advantage of proteasome functions in order to set up a favourable cytoskeletal "environment" for its replication and spread. Furthermore, the relationship between influenza virus and the host cell is likely to depend on both the cellular model and the virus strain.
authors.forename: Flora
authors.initials: F
authors.lastname: De Conto
authors.forename: Carlo
authors.initials: C
authors.lastname: Chezzi
authors.forename: Alessandra
authors.initials: A
authors.lastname: Fazzi
authors.forename: Sergey V
authors.initials: SV
authors.lastname: Razin
authors.forename: Maria Cristina
authors.initials: MC
authors.lastname: Arcangeletti
authors.forename: Maria Cristina
authors.initials: MC
authors.lastname: Medici
authors.forename: Rita
authors.initials: R
authors.lastname: Gatti
authors.forename: Adriana
authors.initials: A
authors.lastname: Calderaro
mesh_terms: D000107:Acetylation / Q000187:drug effects; D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D003599:Cytoskeleton; D004285:Dogs; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000502:physiology*; D007976:Leupeptins / Q000494:pharmacology; D008253:Macaca mulatta; D061985:Madin Darby Canine Kidney Cells; D018613:Microscopy, Confocal; D008870:Microtubules / Q000378:metabolism*; D046988:Proteasome Endopeptidase Complex / Q000737:chemistry / Q000378:metabolism*; D061988:Proteasome Inhibitors / Q000494:pharmacology; D011758:Pyrroles / Q000494:pharmacology; D011759:Pyrrolidines / Q000494:pharmacology; D014404:Tubulin / Q000378:metabolism; D014779:Virus Replication / Q000187:drug effects
chemical_list: C000594744:1-(1-(4-fluorophenyl)-2,5-dimethylpyrrol-3-yl)-2-pyrrolidin-1-ylethanone; D007976:Leupeptins; D061988:Proteasome Inhibitors; D011758:Pyrroles; D011759:Pyrrolidines; D014404:Tubulin; D046988:Proteasome Endopeptidase Complex; C072553:benzyloxycarbonylleucyl-leucyl-leucine aldehyde