title: Reclassification ofEubacterium rectale(Hauduroyet al.1937) Prévot 1938 in a new genusAgathobactergen. nov. asAgathobacter rectaliscomb. nov., and description ofAgathobacter ruminissp. nov., isolated from the rumen contents of sheep and cows.
abstract: Three strains of a butyrate-producing bacterium were isolated from the rumen contents of grazing sheep and cows. The strains were anaerobic, with Gram-positive cell walls, straight-to-slightly-curved, rod-shaped, non-spore-forming and single flagellate. C14 : 1, C14 : 0, C16 : 0and C16 : 1were the predominant fatty acids. The cell-wall peptidoglycan type was A1γ. The DNA G+C content varied from 41.4 to 42.2 mol%. 16S rRNA gene sequence similarities between the isolates andEubacterium rectale,Roseburia hominisandRoseburia intestinaliswere found to be 96, 95 and 95 %, respectively. The phylogenetic tree showed that the strains constituted a different taxon, separate from other taxa with validly published names and forming a cluster with strains ofEubacterium rectale.On the basis of phenotypic, chemotaxonomic and phylogenetic results (16S RNA,dnaK,groEL,atpAgenes), the isolates are considered to represent a novel species of a new genus of the familyLachnospiraceae, for which the nameAgathobacter ruminisgen. nov., sp. nov. is proposed (type strain JK623T= DSM 29029T= LMG 28559T). We also propose the transfer ofEubacterium rectaleto the new genusas Agathobacter rectalisgen. nov., comb nov. This new genus represents saccharoclastic, chemo-organotrophic and obligatory anaerobic, non-spore-forming rods with Gram-positive membrane. The main fermentation products on peptone yeast glucose (PYG) medium were butyrate, acetate, hydrogen and lactate. The type species of the genus isAgathobacter rectalisgen. nov., comb nov. (Prévot, 1938) with type strain ATCC 33656T( = JCM 17463T).
authors.affiliation: Institute of Animal Physiology and Genetics, The Czech Academy of Sciences, v.v.i., Vídenˇská 1083, Prague 4 - Krcˇ, 142 20, Czech Republic.
authors.forename: Jaime A
authors.initials: JA
authors.lastname: Rosero
authors.affiliation: Institute of Animal Physiology and Genetics, The Czech Academy of Sciences, v.v.i., Vídenˇská 1083, Prague 4 - Krcˇ, 142 20, Czech Republic.
authors.forename: Jirˇí
authors.initials: J
authors.lastname: Killer
authors.affiliation: Institute of Animal Physiology and Genetics, The Czech Academy of Sciences, v.v.i., Vídenˇská 1083, Prague 4 - Krcˇ, 142 20, Czech Republic.
authors.forename: Hana
authors.initials: H
authors.lastname: Sechovcová
authors.affiliation: Institute of Animal Physiology and Genetics, The Czech Academy of Sciences, v.v.i., Vídenˇská 1083, Prague 4 - Krcˇ, 142 20, Czech Republic.
authors.forename: Jakub
authors.initials: J
authors.lastname: Mrázek
authors.affiliation: Prague Infrastructure for Structure Biology and Metabolomics II, Institute of Microbiology, The Czech Academy of Sciences, v.v.i., Vídenˇská 1083, 142 20, Prague 4, Czech Republic.
authors.forename: Oldrˇich
authors.initials: O
authors.lastname: Benada
authors.affiliation: Institute of Animal Physiology and Genetics, The Czech Academy of Sciences, v.v.i., Vídenˇská 1083, Prague 4 - Krcˇ, 142 20, Czech Republic.
authors.forename: Katerˇina
authors.initials: K
authors.lastname: Fliegerová
authors.affiliation: Czech University of Life Sciences, Department of Microbiology, Nutrition and Dietetics, Kamýcká 129, Prague 6 - Suchdol, 165 21, Czech Republic.
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Havlík
authors.affiliation: Institute of Animal Physiology and Genetics, The Czech Academy of Sciences, v.v.i., Vídenˇská 1083, Prague 4 - Krcˇ, 142 20, Czech Republic.
authors.forename: Jan
authors.initials: J
authors.lastname: Kopečný