title: The regulation effect of ulinastatin on the expression of SSAT2 and AQP4 in myocardial tissue of rats after cardiopulmonary resuscitation.
abstract: OBJECTIVE
  This study aims to investigate the regulation effects of ulinastatin (UT1) on the expression of spermidine/spermine -N1-acetyltransferase 2 (SSAT2) and aquaporin 4 (AQP4) in myocardial tissue of rats after cardiopulmonary resuscitation (CPR) and their correlations.
  
  
  METHODS
  A total of 90 adult SD rats were divided into sham operation group (A, n=30), model group (B, n=30) and UT1 group (C, n=30). The cardiac arrest (CA) and CPR model was established by asphyxia method. Left ventricular fractional shortening (LVFS), left ventricular ejection fraction (LVEF) and E/A peak ratio of mitral valve in three groups were collected by ultrasonic echocardiography. Apoptosis of myocardial cells was detected by DAPI staining. The expression levels of SSAT2 and AQP4 were detected by RT-PCR, Western blotting and immunohistochemical methods.
  
  
  RESULTS
  UT1 could significantly improve the levels of LVFS, LVEF and E/A ratio and decrease myocardial cell apoptosis. As compared with group B, the expression level of SSAT2 increased and the expression level of AQP4 decreased in group C (P<0.01). SSAT2 was the most in group A and the least in group B while AQP4 was the least in group A and the most in group B (P<0.01). There was positive correlation between SSAT2 and cardiac function in CRP model while there was negative correlation between AQP4 and cardiac function (P<0.01). The expression of SSAT2 and AQP4 protein in myocardial tissue was negatively correlated in CRP model (r=-0.920, P<0.01).
  
  
  CONCLUSIONS
  UT1 can effectively reduce the cardiac function damage caused by CRP, which could be related with the increased SSAT2 and decreased AQP4.
authors.affiliation: Department of Internal Medicine, Emergency Center of Qinghai People's Hospital No. 2 Gonghe Road, Xining, Qinghai 810007, China.
authors.forename: Wujian
authors.initials: W
authors.lastname: He
authors.affiliation: Qinghai Red Cross Hospital Xining, Qinghai, China.
authors.forename: Yufang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Internal Medicine, Emergency Center of Qinghai People's Hospital No. 2 Gonghe Road, Xining, Qinghai 810007, China.
authors.forename: Hongxia
authors.initials: H
authors.lastname: Geng
authors.affiliation: Department of Internal Medicine, Emergency Center of Qinghai People's Hospital No. 2 Gonghe Road, Xining, Qinghai 810007, China.
authors.forename: Yanzhen
authors.initials: Y
authors.lastname: Li
mesh_terms: D000123:Acetyltransferases / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D051401:Aquaporin 4 / Q000235:genetics / Q000378:metabolism*; D016887:Cardiopulmonary Resuscitation / Q000009:adverse effects*; D002316:Cardiotonic Agents / Q000494:pharmacology*; D019610:Cytoprotection; D004195:Disease Models, Animal; D005786:Gene Expression Regulation; D006023:Glycoproteins / Q000494:pharmacology*; D006323:Heart Arrest / Q000000981:diagnostic imaging / Q000201:enzymology / Q000235:genetics / Q000503:physiopathology / Q000628:therapy*; D009200:Myocardial Contraction / Q000187:drug effects; D009206:Myocardium / Q000201:enzymology* / Q000473:pathology; D017207:Rats, Sprague-Dawley; D013318:Stroke Volume / Q000187:drug effects; D014463:Ultrasonography; D016277:Ventricular Function, Left / Q000187:drug effects; D000099228:Diamine N-Acetyltransferase
chemical_list: D000123:Acetyltransferases; D051401:Aquaporin 4; D002316:Cardiotonic Agents; D006023:Glycoproteins; D000099228:Diamine N-Acetyltransferase; C496032:Aqp4 protein, rat; C028665:urinastatin
keywords: Ulinastatin (UT1); aquaporin 4 (AQP4); cardiopulmonary resuscitation (CPR); spermidine/spermine-N1-acetyltransferase 2 (SSAT2)