title: Mechanistic Study on Triptorelin Action in Protecting From 5-FU-Induced Ovarian Damage in Rats.
abstract: Triptorelin, a kind of GnRH agonist, is widely used in the treatment of hormone-responsive cancers in the clinic. This study aimed to discover the underlying mechanism of triptorelin in protection from 5-fluorouracil (5-FU)-induced ovarian damage in Sprague-Dawley rats. In the present study, after using 5-FU to induce ovarian damage in rats, body weight and wet ovaries were weighed, the levels of estradiol (E2), follicle-stimulating hormone (FSH), and anti-Müllerian hormone (AMH) in blood were detected, and the expression of Bcl-2, Bax, and NF-κB was determined. It suggested that, compared to the control, body weight gain, the ratio of ovarian wet weight to body weight, primary follicle numbers, and the levels of AMH were significantly decreased, while the concentration of E2 and FSH was heavily increased following 5-FU administration. In contrast, after coadministration of triptorelin with 5-FU, the ratio of ovarian wet weight to body weight and the levels of AMH were significantly increased, whereas the level of E2 and FSH was decreased significantly when compared with the 5-FU group. Furthermore, at indicated times, 5-FU led to the reduced Bcl-2 and NF-κB expression and increased Bax expression while triptorelin plus 5-FU increased Bcl-2 and NF-κB expression and decreased Bax expression. It was indicated that triptorelin could protect rats from 5-FU-induced ovarian damage by modulation of hormones, Bcl-2, Bax, and NF-κB. These results might highlight the mechanism of triptorelin as a protective agent in clinical chemotherapy for ovarian damage.
authors.affiliation: Obstertrics and Gynaecology, The First Affiliated Hospital of Henan University of Science and Technology, Luoyang, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Tian
authors.forename: Lingxia
authors.initials: L
authors.lastname: Liang
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Ruifang
authors.initials: R
authors.lastname: Wang
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Cheng
authors.forename: Zhen
authors.initials: Z
authors.lastname: Yan
authors.forename: Yawei
authors.initials: Y
authors.lastname: Chen
authors.forename: Pengwei
authors.initials: P
authors.lastname: Qi
mesh_terms: D000818:Animals; D019610:Cytoprotection / Q000187:drug effects* / Q000502:physiology; D005260:Female; D005472:Fluorouracil / Q000633:toxicity*; D018836:Inflammation Mediators / Q000037:antagonists & inhibitors / Q000378:metabolism; D010053:Ovary / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017329:Triptorelin Pamoate / Q000494:pharmacology*
chemical_list: D018836:Inflammation Mediators; D017329:Triptorelin Pamoate; D005472:Fluorouracil