title: Evaluation of Inhibition Efficiency for the Detection of Captan, 2,3,7,8-Tetrachlorodibenzodioxin, Pentachlorophenol and Carbosulfan in Water: An Electrochemical Approach.
abstract: A novel bio-analytical method has been devised based on the change in catalytic activity of acetylcholinesterase (AChE) enzyme induced by captan, carbosulfan, 2,3,7,8-tetrachlorodibenzodioxin (TCDD) and pentachlorophenol (PCP) for the investigation of inhibition efficiency and sensitivity using Pt/ZnO/AChE/Chitosan bioelectrode. The inhibition curves of captan, carbosulfan, TCDD and PCP were similar to Michaelis-Menten curve. TCDD held the minimum inhibitor Michaelis-Menten constant ([Formula: see text]) value (10.2 nM) in comparison with PCP (10.9 nM), carbosulfan (14.5 nM) and captan (7.9 × 10(3) nM). The maximum inhibition of AChE enzyme by captan was about 100 %, which was much higher than that of TCDD (72.7 %), PCP (68.1 %) and carbosulfan (47.7 %). The calculated theoretical sensitivity was in the order of TCDD > PCP > carbosulfan > captan. Comparing with TCDD (35.3 %), PCP (47.8 %) and carbosulfan (20.9 %), only the inhibition efficiency of captan (55.0 %) was the maximum. The developed bioelectrode exhibited high recovery and low relative standard deviation in local tap water samples.
authors.affiliation: Nanosensors Lab, SASTRA University, Thanjavur, Tamil Nadu, 613 401, India.|Centre for Nanotechnology and Advanced Biomaterials (CeNTAB), School of Electrical and Electronics Engineering, SASTRA University, Thanjavur, Tamil Nadu, 613 401, India.|School of Electrical and Electronics Engineering, SASTRA University, Thanjavur, Tamil Nadu, 613 401, India.
authors.forename: Noel
authors.initials: N
authors.lastname: Nesakumar
authors.affiliation: Nanosensors Lab, SASTRA University, Thanjavur, Tamil Nadu, 613 401, India.|Centre for Nanotechnology and Advanced Biomaterials (CeNTAB), School of Electrical and Electronics Engineering, SASTRA University, Thanjavur, Tamil Nadu, 613 401, India.|School of Electrical and Electronics Engineering, SASTRA University, Thanjavur, Tamil Nadu, 613 401, India.
authors.forename: Bhat Lakshmishri
authors.initials: BL
authors.lastname: Ramachandra
authors.affiliation: Centre for Nanotechnology and Advanced Biomaterials (CeNTAB), School of Electrical and Electronics Engineering, SASTRA University, Thanjavur, Tamil Nadu, 613 401, India.|School of Chemical and Biotechnology, SASTRA University, Thanjavur, Tamil Nadu, 613 401, India.
authors.forename: Swaminathan
authors.initials: S
authors.lastname: Sethuraman
authors.affiliation: Centre for Nanotechnology and Advanced Biomaterials (CeNTAB), School of Electrical and Electronics Engineering, SASTRA University, Thanjavur, Tamil Nadu, 613 401, India.|School of Chemical and Biotechnology, SASTRA University, Thanjavur, Tamil Nadu, 613 401, India.
authors.forename: Uma Maheswari
authors.initials: UM
authors.lastname: Krishnan
authors.affiliation: Nanosensors Lab, SASTRA University, Thanjavur, Tamil Nadu, 613 401, India. rjbosco@ece.sastra.edu.|Centre for Nanotechnology and Advanced Biomaterials (CeNTAB), School of Electrical and Electronics Engineering, SASTRA University, Thanjavur, Tamil Nadu, 613 401, India. rjbosco@ece.sastra.edu.|School of Electrical and Electronics Engineering, SASTRA University, Thanjavur, Tamil Nadu, 613 401, India. rjbosco@ece.sastra.edu.
authors.forename: John Bosco Balaguru
authors.initials: JB
authors.lastname: Rayappan
mesh_terms: D000110:Acetylcholinesterase / Q000737:chemistry; D015374:Biosensing Techniques; D002215:Captan / Q000032:analysis* / Q000737:chemistry; D002219:Carbamates / Q000032:analysis* / Q000737:chemistry; D010416:Pentachlorophenol / Q000032:analysis* / Q000737:chemistry; D010573:Pesticide Residues / Q000032:analysis*; D000072317:Polychlorinated Dibenzodioxins / Q000032:analysis* / Q000737:chemistry; D014867:Water / Q000737:chemistry*
chemical_list: D002219:Carbamates; D010573:Pesticide Residues; D000072317:Polychlorinated Dibenzodioxins; D014867:Water; D010416:Pentachlorophenol; D000110:Acetylcholinesterase; D002215:Captan; C035038:carbosulfan
keywords: Acetylcholinesterase; Biosensor; Inhibition efficiency; Maximum inhibition; Michaelis–Menten constant; Sensitivity