title: An HPLC-ECD method for monoamines and metabolites quantification in cuttlefish (cephalopod) brain tissue.
abstract: The cuttlefish belongs to the mollusk class Cephalopoda, considered as the most advanced marine invertebrates and thus widely used as models to study the biology of complex behaviors and cognition, as well as their related neurochemical mechanisms. Surprisingly, methods to quantify the biogenic monoamines and their metabolites in cuttlefish brain remain sparse and measure a limited number of analytes. This work aims to validate an HPLC-ECD method for the simultaneous quantification of dopamine, serotonin, norepinephrine and their main metabolites in cuttlefish brain. In comparison and in order to develop a method suitable to answer both ecological and biomedical questions, the validation was also carried out on a phylogenetically remote species: mouse (mammals). The method was shown to be accurate, precise, selective, repeatable and sensitive over a wide range of concentrations for 5-hydroxyindole-3-acetic acid, serotonin, dopamine, 3,4-dihydroxyphenylacetic acid and norepinephrine in the both extracts of cuttlefish and mouse brain, though with low precision and recovery for 4-hydroxy-3-methoxyphenylethylene glycol. Homovanillic acid, accurately studied in rodents, was not detectable in the brain of cuttlefish. Overall, we described here the first fully validated HPLC method for the routine measurement of both monoamines and metabolites in cuttlefish brain. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: Normandie Université, CS F-14032 Caen, France.|Groupe Mémoire et Plasticité comportementale, EA 4259, Campus Horowitz, Université de Caen Normandie, Esplanade de la Paix, CS F-14032, Caen, cedex, France.
authors.forename: Flavie
authors.initials: F
authors.lastname: Bidel
authors.affiliation: Normandie Université, CS F-14032 Caen, France.|Groupe Mémoire et Plasticité comportementale, EA 4259, Campus Horowitz, Université de Caen Normandie, Esplanade de la Paix, CS F-14032, Caen, cedex, France.
authors.forename: Sophie
authors.initials: S
authors.lastname: Corvaisier
authors.affiliation: Normandie Université, CS F-14032 Caen, France.|Groupe Mémoire et Plasticité comportementale, EA 4259, Campus Horowitz, Université de Caen Normandie, Esplanade de la Paix, CS F-14032, Caen, cedex, France.
authors.forename: Christelle
authors.initials: C
authors.lastname: Jozet-Alves
authors.affiliation: Normandie Université, CS F-14032 Caen, France.|ABTE, EA4651, F-14032, Caen, cedex, France.|Centre François Baclesse, 3 Avenue du Général Harris BP 5026, -14076, Caen, cedex 05, France.
authors.forename: Ivannah
authors.initials: I
authors.lastname: Pottier
authors.affiliation: Normandie Université, CS F-14032 Caen, France.|Groupe Mémoire et Plasticité comportementale, EA 4259, Campus Horowitz, Université de Caen Normandie, Esplanade de la Paix, CS F-14032, Caen, cedex, France.
authors.forename: François
authors.initials: F
authors.lastname: Dauphin
authors.affiliation: Normandie Université, CS F-14032 Caen, France.|Groupe Mémoire et Plasticité comportementale, EA 4259, Campus Horowitz, Université de Caen Normandie, Esplanade de la Paix, CS F-14032, Caen, cedex, France.
authors.forename: Nadège
authors.initials: N
authors.lastname: Naud
authors.affiliation: Normandie Université, CS F-14032 Caen, France.|Groupe Mémoire et Plasticité comportementale, EA 4259, Campus Horowitz, Université de Caen Normandie, Esplanade de la Paix, CS F-14032, Caen, cedex, France.
authors.forename: Cécile
authors.initials: C
authors.lastname: Bellanger
mesh_terms: D000818:Animals; D015306:Biogenic Monoamines / Q000378:metabolism*; D001921:Brain / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D049832:Decapodiformes; D055664:Electrochemical Techniques / Q000379:methods*; D057230:Limit of Detection; D051379:Mice; D012015:Reference Standards; D015203:Reproducibility of Results
chemical_list: D015306:Biogenic Monoamines
keywords: biogenic monoamines; cephalopods; dopamine; high-performance liquid chromatography-electrochemical detection; rodents; serotonin