title: Crystal structure of the GTPase domain and the bundle signalling element of dynamin in the GDP state.
abstract: Dynamin is the prototype of a family of large multi-domain GTPases. The 100 kDa protein is a key player in clathrin-mediated endocytosis, where it cleaves off vesicles from membranes using the energy from GTP hydrolysis. We have solved the high resolution crystal structure of a fusion protein of the GTPase domain and the bundle signalling element (BSE) of dynamin 1 liganded with GDP. The structure provides a hitherto missing snapshot of the GDP state of the hydrolytic cycle of dynamin and reveals how the switch I region moves away from the active site after GTP hydrolysis and release of inorganic phosphate. Comparing our structure of the GDP state with the known structures of the GTP state, the transition state and the nucleotide-free state of dynamin 1 we describe the structural changes through the hydrolytic cycle.
authors.affiliation: Hannover Medical School, Institute for Biophysical Chemistry, Carl-Neuberg-Str. 1, 30625 Hannover, Germany.
authors.forename: Roopsee
authors.initials: R
authors.lastname: Anand
authors.affiliation: Hannover Medical School, Institute for Biophysical Chemistry, Carl-Neuberg-Str. 1, 30625 Hannover, Germany.
authors.forename: Susanne
authors.initials: S
authors.lastname: Eschenburg
authors.affiliation: Hannover Medical School, Institute for Biophysical Chemistry, Carl-Neuberg-Str. 1, 30625 Hannover, Germany. Electronic address: Reubold.Thomas@mh-hannover.de.
authors.forename: Thomas F
authors.initials: TF
authors.lastname: Reubold
mesh_terms: D001665:Binding Sites; D003461:Crystallography; D034281:Dynamins / Q000737:chemistry* / Q000648:ultrastructure*; D004789:Enzyme Activation; D020558:GTP Phosphohydrolases / Q000737:chemistry* / Q000648:ultrastructure*; D006153:Guanosine Diphosphate / Q000737:chemistry*; D062105:Molecular Docking Simulation; D011485:Protein Binding; D011487:Protein Conformation; D017434:Protein Structure, Tertiary
chemical_list: D006153:Guanosine Diphosphate; D020558:GTP Phosphohydrolases; D034281:Dynamins
keywords: Dynamin; Endocytosis; GTPase; Hydrolytic cycle; X-ray structure