title: Effect of Acer tegmentosum bark on atopic dermatitis-like skin lesions in NC/Nga mice.
abstract: BACKGROUND
  Atopic dermatitis (AD) is a chronic and relapsing inflammatory condition characterized by pruritic and eczematous skin lesions that requires safe and effective pharmacological therapy. The bark of Acer tegmentosum Maxim trees has been used in Korean folk and traditional medicine to treat abscesses, surgical bleeding, liver diseases, and AD.
  
  
  OBJECTIVE
  To investigate the therapeutic effect of A. tegmentosum, on a mouse model of Dermatophagoides farinae (Df)-induced AD.
  
  
  METHODS
  Development of AD-like skin lesions was induced by repetitive skin contact with barrier-disrupted backs of NC/Nga mice with Df body ointment, and the effects of A. tegmentosum were evaluated on the basis of histopathological skin assessment results, ear swelling, and cytokine production in the dorsal skin. The component of A. tegmentosum, salidroside, inhibited the production of TSLP in KCMH-1 cells, which indicated that its production could be pharmacologically regulated.
  
  
  RESULTS
  Topical application of A. tegmentosum for 1 week after Df body ointment challenge significantly reduced ear swelling and improved dorsal skin lesions. Suppression of dermatitis by combined therapy was accompanied by a decrease in the skin level of Th2 cytokines, such as interleukin (IL)-4, IL-5 and IL-13, plasma levels of thymus and activation-regulated chemokine, and IgE. Induction of thymic stromal lymphopoietin, which leads to a systemic Th2 response, was also reduced in in vivo and in vitro by A. tegmentosum and salidroside.
  
  
  CONCLUSIONS
  Our findings suggest that A. tegmentosum treatment has a significant therapeutic effect on Df-induced AD-like skin lesions on NC/Nga mice through inhibition of thymic stromal lymphopoietin and IgE via a mechanism that may inhibit Th2-mediated immune responses. These results suggest that A. tegmentosum and salidroside may be useful tools for the treatment of AD.
authors.affiliation: Department of Herbology, College of Korean Medicine, Kyung Hee University, Republic of Korea; Integrated Research Institute of Pharmaceutical Sciences, College of Pharmacy, The Catholic University of Korea, Bucheon 420-743, Republic of Korea.
authors.forename: Gabsik
authors.initials: G
authors.lastname: Yang
authors.affiliation: Department of Herbology, College of Korean Medicine, Kyung Hee University, Republic of Korea.
authors.forename: Duckgun
authors.initials: D
authors.lastname: An
authors.affiliation: Department of Herbology, College of Korean Medicine, Kyung Hee University, Republic of Korea.
authors.forename: Mi-Hwa
authors.initials: MH
authors.lastname: Lee
authors.affiliation: Department of Herbology, College of Korean Medicine, Kyung Hee University, Republic of Korea.
authors.forename: Kyungjin
authors.initials: K
authors.lastname: Lee
authors.affiliation: Department of Herbology, College of Korean Medicine, Kyung Hee University, Republic of Korea.
authors.forename: Bumjung
authors.initials: B
authors.lastname: Kim
authors.affiliation: Department of Herbology, College of Korean Medicine, Kyung Hee University, Republic of Korea.
authors.forename: Chinannai Khanita
authors.initials: CK
authors.lastname: Suman
authors.affiliation: Department of Herbology, College of Korean Medicine, Kyung Hee University, Republic of Korea.
authors.forename: Inhye
authors.initials: I
authors.lastname: Ham
authors.affiliation: Department of Herbology, College of Korean Medicine, Kyung Hee University, Republic of Korea. Electronic address: hychoi@khu.ac.kr.
authors.forename: Ho-Young
authors.initials: HY
authors.lastname: Choi
mesh_terms: D031002:Acer / Q000737:chemistry*; D000818:Animals; D039741:Antigens, Dermatophagoides / Q000009:adverse effects*; D045744:Cell Line, Tumor; D054414:Chemokine CCL17 / Q000235:genetics / Q000378:metabolism; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D003876:Dermatitis, Atopic / Q000188:drug therapy*; D007073:Immunoglobulin E / Q000097:blood / Q000378:metabolism; D008297:Male; D008407:Mast Cells / Q000378:metabolism; D051379:Mice; D008815:Mice, Inbred Strains; D024301:Plant Bark / Q000737:chemistry*; D000094632:Thymic Stromal Lymphopoietin
chemical_list: D039741:Antigens, Dermatophagoides; C499538:Ccl17 protein, mouse; D054414:Chemokine CCL17; D016207:Cytokines; D007073:Immunoglobulin E; D000094632:Thymic Stromal Lymphopoietin
keywords: Acer tegmentosum; Atopic dermatitis; Dermatophagoides farina; Salidroside; Thymic stromal lymphopoietin