title: [Erythrocytic parameters Sysmex in a case of severe haemolysis].
abstract: We are reporting a case of severe haemolytic anemia with cold agglutinins which combines several spurious determinations. It shows the usefulness of the new erythrocytic parameters of the XE 5000 Sysmex, specially: red blood cells with optical count (RBC-O), GR-He (intra-erythocytic hemoglobin) and R-MFV (most frequent volume). Optical red blood cells act as a substitute for red cells count instead of impedance red cells and R-MFV as a substitute for MCV (mean cell volume). The hematocrit (HCT) is corrected thanks to the following formula: HCT=(RBC-O X R- MFV)/1000. Free plasmatic hemoglobin is included in the measure of hemoglobin by the analyzer but is not available for tissue oxygenation. So, hemoglobin (HGB) has to be corrected by the means of GR- He thanks to the following formula: HGB=(GR He x RBC-O)/10.
authors.affiliation: Laboratoires d'hématologie et de biochimie, Hôpital Pasteur, Nice, France.
authors.forename: Corinne
authors.initials: C
authors.lastname: Ferrero-Vacher
authors.affiliation: Laboratoires d'hématologie et de biochimie, Hôpital Pasteur, Nice, France.
authors.forename: Jean-Éric
authors.initials: JÉ
authors.lastname: Senlis
authors.affiliation: Laboratoires d'hématologie et de biochimie, Hôpital Pasteur, Nice, France.
authors.forename: Denis
authors.initials: D
authors.lastname: Loustaunau
authors.affiliation: Laboratoires d'hématologie et de biochimie, Hôpital Pasteur, Nice, France.
authors.forename: Danièle
authors.initials: D
authors.lastname: Aquaronne
authors.affiliation: Service de médecine interne, Hôpital de l'Archet I, Nice, France.
authors.forename: Pierre-Yves
authors.initials: PY
authors.lastname: Jeandel
authors.affiliation: Laboratoires d'hématologie et de biochimie, Hôpital Pasteur, Nice, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Sudaka
mesh_terms: D000293:Adolescent; D000743:Anemia, Hemolytic / Q000097:blood* / Q000150:complications / Q000473:pathology; D001327:Autoimmune Diseases / Q000097:blood / Q000150:complications; D003450:Cryoglobulins / Q000032:analysis; D004906:Erythrocyte Count / Q000295:instrumentation / Q000379:methods; D004909:Erythrocyte Indices; D004912:Erythrocytes / Q000473:pathology*; D005260:Female; D006400:Hematocrit; D006454:Hemoglobins / Q000032:analysis; D006461:Hemolysis; D006801:Humans; D012720:Severity of Illness Index
chemical_list: D003450:Cryoglobulins; D006454:Hemoglobins; C029443:cold agglutinins
keywords: GR-He; XE 5000®; auto-immune hemolytic anemia; cold agglutinins; optical red blood cells