title: Simultaneous determination of three triterpenes in rat plasma by LC-MS/MS and its application to a pharmacokinetic study of Rhizoma Alismatis extract.
abstract: We have developed a sensitive and specific LC-MS/MS method for the simultaneous determination of alisol A (A), alisol A 23-acetate (A23) and alisol A 24-acetate (A24), the major active components in Rhizoma Alismatis extract (RAE), in rat plasma. In brief, plasma samples were extracted by methyl tert-butyl ether and chromatographically separated by using a C18 column. A tandem mass spectrometric detection with an electrospray ionization (ESI) interface was conducted via multiple reaction monitoring (MRM) under positive ionization mode. This method was validated for specificity, linearity, accuracy (within ±15.4%), intra- and inter-day precision (CV<11.4%) over the concentration range of 25-5000ng/mL for A, and 5-1000ng/mL for both A23 and A24. The significantly lower detection limit was determined as 25ng/mL for A, 5ng/mL for A23 and A24. This validated method of ours was then used to study the pharmacokinetics of RAE in rat. The elimination half-lives (t1/2) of A, A23 and A24 was determined as 0.75, 0.83 and 0.82h respectively after intravenous injection, and the oral absolute bioavailability of A, A23 and A24 was 43.1±18.1%, 6.3±1.5% and 7.9±1.2%. This new determination method of us for alisols is proven to very useful to study the pharmacological activities of RAE in future.
authors.affiliation: China State Institute of Pharmaceutical Industry, National Pharmaceutical Engineering Research Center No. 1111, Halei Road, Zhangjiang Hi-Tech Park, Pudong New District, Shanghai 201203, PR China. Electronic address: chengzh@nperc.com.
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Cheng
authors.affiliation: China State Institute of Pharmaceutical Industry, National Pharmaceutical Engineering Research Center No. 1111, Halei Road, Zhangjiang Hi-Tech Park, Pudong New District, Shanghai 201203, PR China.
authors.forename: Cungang
authors.initials: C
authors.lastname: Ding
authors.affiliation: China State Institute of Pharmaceutical Industry, National Pharmaceutical Engineering Research Center No. 1111, Halei Road, Zhangjiang Hi-Tech Park, Pudong New District, Shanghai 201203, PR China.
authors.forename: Zhou
authors.initials: Z
authors.lastname: Li
authors.affiliation: China State Institute of Pharmaceutical Industry, National Pharmaceutical Engineering Research Center No. 1111, Halei Road, Zhangjiang Hi-Tech Park, Pudong New District, Shanghai 201203, PR China.
authors.forename: Dingzhong
authors.initials: D
authors.lastname: Song
authors.affiliation: China State Institute of Pharmaceutical Industry, National Pharmaceutical Engineering Research Center No. 1111, Halei Road, Zhangjiang Hi-Tech Park, Pudong New District, Shanghai 201203, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Yuan
authors.affiliation: China State Institute of Pharmaceutical Industry, National Pharmaceutical Engineering Research Center No. 1111, Halei Road, Zhangjiang Hi-Tech Park, Pudong New District, Shanghai 201203, PR China.
authors.forename: Wusi
authors.initials: W
authors.lastname: Hao
authors.affiliation: China State Institute of Pharmaceutical Industry, National Pharmaceutical Engineering Research Center No. 1111, Halei Road, Zhangjiang Hi-Tech Park, Pudong New District, Shanghai 201203, PR China.
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Ge
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry / Q000379:methods; D014315:Triterpenes / Q000097:blood*
chemical_list: D014315:Triterpenes
keywords: Alisol A; Alisol A 23-acetate; Alisol A 24-acetate; LC–MS/MS; Pharmacokinetics; Rhizoma Alismatis extract