title: Population genetic structure in Phyla scaberrima from Mexico and Colombia assessed by AFLP markers and implications for conservation.
abstract: Phyla scaberrima (Verbenaceae) is a herbaceous perennial species that is distributed from Mexico (center of origin) to Colombia, growing in forest and swamp edges or grasslands from sea level up to an altitude of 1800 m. The chemical properties and uses in popular medicine have drastically affected the population size of this species. In this study, we investigated genetic variability in populations of P. scaberrima using AFLP markers. Three AFLP primer combinations rendered a total of 997 markers in a sample of 131 individuals from five populations, including two populations from Mexico and three from Colombia. The average percentage of polymorphic loci, gene diversity and Shannon-Wiener index were 46.62, 0.0695, and 0.119, respectively. Analysis of molecular variance showed that the distribution of the genetic variability within populations (85.41%) was higher than between groups (8.11%) and between populations (6.48%). Principal coordinate analysis and Bayesian analysis for the K number of clusters showed that the individuals were dispersed in five (K= 5) clusters. The low levels of genetic diversity observed in these populations demonstrated that the populations from Mexico and Colombia need urgent management to recover their genetic variability.
authors.affiliation: Departamento de Biologia Geral, Universidade Estadual de Londrina, Londrina, PR, Brasil.
authors.forename: L G
authors.initials: LG
authors.lastname: Androcioli
authors.affiliation: Departamento de Biologia Geral, Universidade Estadual de Londrina, Londrina, PR, Brasil edu_wicca@yahoo.com.br.
authors.forename: E A
authors.initials: EA
authors.lastname: Ruas
authors.affiliation: Programa de Pós-Graduação em Agronomia, Departamento de Agronomia, Universidade Estadual de Londrina, Londrina, PR, Brasil.
authors.forename: L A
authors.initials: LA
authors.lastname: Rodrigues
authors.forename: C F
authors.initials: CF
authors.lastname: Ruas
authors.affiliation: Programa de Pós-Graduação em Agronomia, Departamento de Agronomia, Universidade Estadual de Londrina, Londrina, PR, Brasil.
authors.forename: H E R
authors.initials: HE
authors.lastname: Perilla
authors.affiliation: Departamento de Biologia Geral, Universidade Estadual de Londrina, Londrina, PR, Brasil.
authors.forename: P M
authors.initials: PM
authors.lastname: Ruas
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis; D001499:Bayes Theorem; D016000:Cluster Analysis; D003105:Colombia; D003247:Conservation of Natural Resources; D005819:Genetic Markers; D014644:Genetic Variation; D005828:Genetics, Population; D008800:Mexico; D011110:Polymorphism, Genetic; D029648:Verbenaceae / Q000145:classification / Q000235:genetics*
chemical_list: D005819:Genetic Markers