title: [Rapid Identification Method Positive Blood Culture Bottles with MALDI Biotyper].
abstract: Identification method positive blood culture bottles with MALDI Biotyper is the most important test on precisely and rapidly, for detamination the bacterial name in sepsis and bacteremia is very significant for decision a cure. This time, we devised a new method "blend" to identify the mixture hypostasis that were come into being by centrifuging blood culture broths and 70% formic acid with MALDI Biotyper (Bruker). This time, we identified 65 samples rapidly with MALDI biotyper by "on plate" and "blend," and verified their effectivity. As a result of six ways (on plate, blend-3, blend-6, blend-9, blend-12, blend-15), the highest detection rate was Gram negative rods: blend-15 (74.1%), Gram positive cocci: blend-9 (56.3%), total: blend-9 (55.4%). Moreover, we confirmed that the detection rate raised to 85.2% (GNR), 71.0% (GPC) and 77.6% (total), and the usefully was suggested. Our invented method is more excellently than recommended on Gram negative rods, especially Enterobacteriaceae, Staphylococcus aureus, and Enterococcus spp.
authors.affiliation: Department of Clinical Laboratory, Ehime University Hospital.
authors.forename: Mina
authors.initials: M
authors.lastname: Fukuoka
authors.affiliation: Department of Clinical Laboratory, Ehime University Hospital.
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Miyamoto
authors.affiliation: Department of Clinical Laboratory, Ehime University Hospital.
authors.forename: Shinobu
authors.initials: S
authors.lastname: Murakami
authors.affiliation: Department of Clinical Laboratory, Ehime University Hospital.
authors.forename: Yuri
authors.initials: Y
authors.lastname: Tanaka
authors.affiliation: Department of Clinical Laboratory, Ehime University Hospital.
authors.forename: Takuya
authors.initials: T
authors.lastname: Kondo
authors.affiliation: Department of Clinical Laboratory, Ehime University Hospital.
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Nishimiya
authors.affiliation: Department of Clinical Laboratory, Ehime University Hospital.
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Osawa
mesh_terms: D016470:Bacteremia / Q000175:diagnosis* / Q000382:microbiology*; D001431:Bacteriological Techniques; D006801:Humans; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*