title: Mesenchymal-epithelial interaction techniques.
abstract: This paper reviews the importance of mesenchymal-epithelial interactions in development and gives detailed technical protocols for investigating these interactions. Successful analysis of mesenchymal-epithelial interactions requires knowing the ages in which embryonic, neonatal and adult organs can be separated into mesenchymal and epithelial tissues. Methods for separation of mesenchymal and epithelial tissues and preparation of tissue recombinants are described.
authors.affiliation: Department of Urology, University of California, Box 0738, San Francisco, CA 94143, United States. Electronic address: Gerald.Cunha@ucsf.edu.
authors.forename: Gerald R
authors.initials: GR
authors.lastname: Cunha
authors.affiliation: Department of Urology, University of California, Box 0738, San Francisco, CA 94143, United States.
authors.forename: Lawrence
authors.initials: L
authors.lastname: Baskin
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics*; D065150:Cellular Reprogramming / Q000235:genetics; D004848:Epithelium / Q000254:growth & development*; D006801:Humans; D008648:Mesoderm / Q000254:growth & development*; D051379:Mice; D038081:Organogenesis / Q000235:genetics
keywords: Development; Differentiation; Epithelium; Mesenchymal-epithelial interactions; Mesenchyme; Tissue recombinants