title: Direct saliva transcript analysis as a novel non-invasive method for oestrus marker detection in buffaloes.
abstract: Salivary RNA-based biomarkers are not available for any physiological condition in farm animals. Hence, an objective of this study was to perform salivary transcript analysis in buffaloes. Saliva, after removal of the cells and particulate matter, was directly used for RT-PCR without RNA isolation. Direct saliva transcript analysis (DSTA) showed a suggestively significant higher expression of the Heat shock protein 70 (HSP70) and Toll-like receptor 4 (TLR4) at oestrus than the diestrous period in buffaloes by a non-parametric Mann-Whitney U test. Therefore, DSTA without RNA isolation is an easy method to identify salivary RNA markers for oestrus detection in buffaloes.
authors.affiliation: a Molecular Endocrinology, Functional Genomics and Systems Biology Lab, Animal Biochemistry Division, National Dairy Research Institute (NDRI) , Karnal , Haryana , India and.
authors.forename: Suneel Kumar
authors.initials: SK
authors.lastname: Onteru
authors.affiliation: a Molecular Endocrinology, Functional Genomics and Systems Biology Lab, Animal Biochemistry Division, National Dairy Research Institute (NDRI) , Karnal , Haryana , India and.
authors.forename: Vijay Simha
authors.initials: VS
authors.lastname: Baddela
authors.affiliation: a Molecular Endocrinology, Functional Genomics and Systems Biology Lab, Animal Biochemistry Division, National Dairy Research Institute (NDRI) , Karnal , Haryana , India and.
authors.forename: Ravinder
authors.initials: R
authors.lastname: Ravinder
authors.affiliation: a Molecular Endocrinology, Functional Genomics and Systems Biology Lab, Animal Biochemistry Division, National Dairy Research Institute (NDRI) , Karnal , Haryana , India and.
authors.forename: Onnureddy
authors.initials: O
authors.lastname: Kaipa
authors.affiliation: a Molecular Endocrinology, Functional Genomics and Systems Biology Lab, Animal Biochemistry Division, National Dairy Research Institute (NDRI) , Karnal , Haryana , India and.
authors.forename: Varij
authors.initials: V
authors.lastname: Nayan
authors.affiliation: a Molecular Endocrinology, Functional Genomics and Systems Biology Lab, Animal Biochemistry Division, National Dairy Research Institute (NDRI) , Karnal , Haryana , India and.
authors.forename: Prashant
authors.initials: P
authors.lastname: Singh
authors.affiliation: b Division of Livestock Production and Management , National Dairy Research Institute (NDRI) , Karnal , Haryana , India.
authors.forename: Rubina Kumari
authors.initials: RK
authors.lastname: Baithalu
authors.affiliation: a Molecular Endocrinology, Functional Genomics and Systems Biology Lab, Animal Biochemistry Division, National Dairy Research Institute (NDRI) , Karnal , Haryana , India and.
authors.forename: Dheer
authors.initials: D
authors.lastname: Singh
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002020:Buffaloes / Q000235:genetics*; D004971:Estrus / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012463:Saliva / Q000378:metabolism*; D051197:Toll-Like Receptor 4 / Q000235:genetics; D014158:Transcription, Genetic / Q000235:genetics*
chemical_list: D015415:Biomarkers; D018840:HSP70 Heat-Shock Proteins; D051197:Toll-Like Receptor 4
keywords: HSP70; TLR4; salivary-RNA