title: Assessment of effect of Zhu-tan Tong-luo decoction on CYP450 isoforms activity of rats.
abstract: In order to investigate the effects of Zhu-tan Tong-luo decoction on the metabolic capacity of cytochrome P450 (CYP) enzymes, a cocktail method was employed to evaluate the activities of CYP2B6, CYP2C19, CYP1A2, CYP3A4, CYP2C9, CYP2D6. The rats were randomly divided into acute Zhu-Tan Tong-Luo decoction group (Low, High), chronic Zhu-Tan Tong-Luo decoction group (Low, High) and control group. The acute group rats were given 0.6, 1.2 g/kg (Low, High) Zhu-tan Tong-luo decoction by intragastric administration for 1 day, and the chronic group for 14 days. Six probe drugs bupropion, omeprazole, phenacetin, testosterone, tolbutamide, and metroprolol were given to rats through intragastric administration, and the plasma concentrations were determined by UPLC-MS/MS. There statistical pharmacokinetics differences for omeprazole, phenacetin, testosterone, tolbutamide, and metroprolol in rats were observed by comparing acute Zhu-tan Tong-luo decoction group with control group; and statistical pharmacokinetics differences for bupropion, omeprazole, phenacetin, testosterone, tolbutamide, and metroprolol were observed by comparing chronic Zhu-Tan Tong-Luo decoction group with control group. After intragastric administration of Zhu-Tan Tong-Luo decoction may slightly induce the activities of CYP2B6, CYP2C19, CYP1A2, CYP3A4, CYP2C9, CYP2D6 of rats. Induction of drug metabolizing enzyme by Zhu-Tan Tong-Luo decoction would reduce the efficacy of other drug. Additional, there no statistical difference for biochemical results after 1 or 14 intragastric administration of Zhu-Tan Tong-Luo decoction.
authors.affiliation: Department of Rehabilitation, Wenzhou Chinese Medicine Hospital Wenzhou 325005, China.
authors.forename: Yongxi
authors.initials: Y
authors.lastname: Jin
authors.affiliation: Department of Nephrology, Wenzhou Central Hospital Wenzhou 325000, China.
authors.forename: Lingjiu
authors.initials: L
authors.lastname: Shao
authors.affiliation: Department of Pharmacology, Ningbo College of Health & Science Ningbo 315100, China.
authors.forename: Gaowen
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Rehabilitation, The First Affiliated Hospital of Wenzhou Medical University Wenzhou 325000, China.
authors.forename: Mengmeng
authors.initials: M
authors.lastname: Shao
authors.affiliation: Department of Rehabilitation, Wenzhou Chinese Medicine Hospital Wenzhou 325005, China.
authors.forename: Yinghao
authors.initials: Y
authors.lastname: Zhi
authors.affiliation: Department of Rehabilitation, Wenzhou Chinese Medicine Hospital Wenzhou 325005, China.
authors.forename: Wenzong
authors.initials: W
authors.lastname: Zhu
keywords: CYP450; UPLC-MS/MS; cocktail; rat; zhu-tan tong-luo decoction