title: In vitro biological activity of secondary metabolites from Seseli rigidum Waldst. et Kit. (Apiaceae).
abstract: The antioxidant, antimicrobial activity, total phenolic content and flavonoid concentration of Seseli rigidum Waldst. et Kit. were evaluated. Five different extracts of the aboveground plant parts were obtained by extraction with distilled water, methanol, acetone, ethyl acetate and petroleum ether. Total phenols were determined using the Folin-Ciocalteu's reagent, with the highest values obtained in the acetone extract (102.13 mg GAE/g). The concentration of flavonoids, determined by using a spectrophotometric method with aluminum chloride and expressed in terms of rutin equivalent, was also highest in the acetone extracts (291.58 mg RUE/g). The antioxidant activity was determined in vitro using DPPH reagent. The greatest antioxidant activity was expressed in the aqueous extract (46.15 μg/ml). In vitro antimicrobial activities were determined using a microdilution analysis method; minimum inhibitory concentration (MIC) and minimum microbicidal concentration (MMC) were determined. Methanolic extract had the greatest influence on bacilli (MIC at 0.0391 mg/ml), but the best antimicrobial effect had acetone and ethyl acetate extracts considering their broad impact on bacteria. According to our research, S. rigidum can be regarded as promising candidate for natural plant source with high value of biological compounds.
authors.affiliation: Department of Biology and Ecology, Faculty of Science, University of Kragujevac , Radoja Domanovića No. 12, 34000 Kragujevac , Republic of Serbia.
authors.forename: Dragana
authors.initials: D
authors.lastname: Jakovljević
authors.affiliation: Department of Biology and Ecology, Faculty of Science, University of Kragujevac , Radoja Domanovića No. 12, 34000 Kragujevac , Republic of Serbia.
authors.forename: Sava
authors.initials: S
authors.lastname: Vasić
authors.affiliation: Department of Biology and Ecology, Faculty of Science, University of Kragujevac , Radoja Domanovića No. 12, 34000 Kragujevac , Republic of Serbia.
authors.forename: Milan
authors.initials: M
authors.lastname: Stanković
authors.affiliation: Department of Biology and Ecology, Faculty of Science, University of Kragujevac , Radoja Domanovića No. 12, 34000 Kragujevac , Republic of Serbia.
authors.forename: Ljiljana
authors.initials: L
authors.lastname: Čomić
authors.affiliation: Department of Biology and Ecology, Faculty of Science, University of Kragujevac , Radoja Domanovića No. 12, 34000 Kragujevac , Republic of Serbia.
authors.forename: Marina
authors.initials: M
authors.lastname: Topuzović
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000302:isolation & purification; D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification; D019661:Apiaceae / Q000737:chemistry*; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification; D008826:Microbial Sensitivity Tests; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification; D010936:Plant Extracts / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D000975:Antioxidants; D005419:Flavonoids; D010636:Phenols; D010936:Plant Extracts
keywords: Antioxidants; Seseli rigidum; antimicrobial activity; flavonoids; phenols