title: LumeNEXT: A Practical Method to Pattern Luminal Structures in ECM Gels.
abstract: In vitro biomimetic modeling of physio-logical structures bridges the gap between 2D in vitro culture and animal models. Lumens (tubular structures) are ubiquitous in vivo, being present in blood vessels, mammary ducts, and the lymphatic system. A method 'LumeNEXT' is presented here that allows the fabrication of 3D embedded lumens where size, structure, distance, and configuration can be controlled using standard poly-dimethylsiloxane micromolding methods.
authors.affiliation: Microtechnology, Medicine and Biology Lab, Biomedical Engineering, College of Engineering, University of Wisconsin-Madison, Madison, WI, 53705, USA.
authors.forename: José A
authors.initials: JA
authors.lastname: Jiménez-Torres
authors.affiliation: Microtechnology, Medicine and Biology Lab, Biomedical Engineering, College of Engineering, University of Wisconsin-Madison, Madison, WI, 53705, USA.
authors.forename: Stephen L
authors.initials: SL
authors.lastname: Peery
authors.affiliation: Microtechnology, Medicine and Biology Lab, Biomedical Engineering, College of Engineering, University of Wisconsin-Madison, Madison, WI, 53705, USA.
authors.forename: Kyung E
authors.initials: KE
authors.lastname: Sung
authors.affiliation: Microtechnology, Medicine and Biology Lab, Biomedical Engineering, College of Engineering, University of Wisconsin-Madison, Madison, WI, 53705, USA.
authors.forename: David J
authors.initials: DJ
authors.lastname: Beebe
mesh_terms: D000818:Animals; D048430:Cell Shape; D005109:Extracellular Matrix / Q000378:metabolism*; D005782:Gels / Q000737:chemistry*; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology; D006801:Humans; D008280:Magnetics; D051381:Rats; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D005782:Gels
keywords: angiogenesis; extracellular matrix; lumens; microfluidics; organotypic models