title: Bacillus oceanisp. nov., isolated from seawater.
abstract: Two Gram-staining-positive, endospore-forming, rod-shaped bacterial strains, designated strain SW109Tand strain W006, were isolated from a seawater sample collected from the Indian Ocean. The strains were strictly aerobic, catalase- and oxidase-positive and motile by peritrichous flagella. The predominant cellular fatty acids (>10 %) were anteiso-C15 : 0, iso-C15 : 0and iso-C14 : 0. The major menaquinone was menaquinone-7 (MK-7) and the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The genomic DNA G+C content of strain SW109Tand strain W006 was 46.3 and 46.1 mol%, respectively. Phylogenetic analyses based on 16S rRNA gene sequences indicated that the two strains represented a novel member of the genusBacillus, showing the highest similarity withBacillus haloduransLMG 7121TandBacillus okuhidensisDSM 13666T(96.4 and 96.2 % sequence similarity, respectively). On the basis of phylogenetic inference and phenotypic characteristics, it is proposed that the two strains represent a novel species of the genusBacillus, for which the nameBacillus oceanisp. nov. is proposed. The type strain is SW109T( = CGMCC 1.12347T= DSM 100579T).
authors.affiliation: China General Microbiological Culture Collection Center,Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PRChina.
authors.forename: Lei
authors.initials: L
authors.lastname: Song
authors.affiliation: China General Microbiological Culture Collection Center,Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PRChina.
authors.forename: Hongcan
authors.initials: H
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Microbial Resources,Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PRChina.
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Microbial Resources,Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PRChina.
authors.forename: Ying
authors.initials: Y
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Microbial Resources,Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PRChina.
authors.forename: Xin
authors.initials: X
authors.lastname: Dai
authors.affiliation: Key Laboratory of Submarine Geosciences,Second Institute of Oceanography, State Oceanic Administration, Hangzhou 310012, PRChina.
authors.forename: Xiqiu
authors.initials: X
authors.lastname: Han
authors.affiliation: China General Microbiological Culture Collection Center,Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PRChina.
authors.forename: Yuguang
authors.initials: Y
authors.lastname: Zhou