title: Oral Administration of Achyranthis radix Extract Prevents TMA-induced Allergic Contact Dermatitis by Regulating Th2 Cytokine and Chemokine Production in Vivo.
abstract: Allergic contact dermatitis (ACD) remains a major skin disease in many countries, necessitating the discovery of novel and effective anti-ACD agents. In this study, we investigated the preventive effects of Achyranthis radix extract (AcRE) on trimellitic anhydride (TMA)-induced dermatitis and the potential mechanism of action involved. Oral administration of AcRE and prednisolone (PS) significantly suppressed TMA-induced increases in ear and epidermal thickness, and IgE expression. In addition, abnormal expression of IL-1β and TNF-α protein and mRNA was also significantly attenuated by oral administration of AcRE. Treatment with AcRE also significantly suppressed TMA-induced IL-4 and IL-13 cytokines and mRNA expression in vivo. Moreover, AcRE strongly suppressed TMA-induced IL-4 and IL-5 production in draining lymph nodes, as well as OVA-induced IL-4 and IL-5 expression in primary cultured splenocytes. Interestingly, AcRE suppressed IL-4-induced STAT6 phosphorylation in both primary cultured splenocytes and HaCaT cells, and TMA-induced GATA3 mRNA expression ex vivo. AcRE also suppressed TMA-mediated CCL11 and IL-4-induced CCL26 mRNA expression and infiltration of CCR3 positive cells. The major compounds from AcRE were identified as gentisic acid (0.64 ± 0.2 μg/g dry weight of AcRE), protocatechuic acid (2.69 ± 0.1 μg/g dry weight of AcRE), 4-hydroxybenzoic acid (5.59 ± 0.3 μg/g dry weight of AcRE), caffeic acid (4.21 ± 0.1 μg/g dry weight of AcRE), and ferulic acid (14.78 ± 0.4 ± 0.3 μg/g dry weight of AcRE). Taken together, these results suggest that AcRE has potential for development as an agent to prevent and treat allergic contact dermatitis.
authors.affiliation: Research Group of Nutraceuticals for Metabolic Syndrome, Korea Food Research Institute, Seongnam 13539, Korea. skjung@kfri.re.kr.|Food Biotechnology Program, Korea University of Science and Technology, Daejeon 34113, Korea. skjung@kfri.re.kr.
authors.forename: Sung Keun
authors.initials: SK
authors.lastname: Jung
authors.affiliation: Research Group of Nutraceuticals for Metabolic Syndrome, Korea Food Research Institute, Seongnam 13539, Korea. choidw19@gmail.com.|Food Biotechnology Program, Korea University of Science and Technology, Daejeon 34113, Korea. choidw19@gmail.com.
authors.forename: Dae Woon
authors.initials: DW
authors.lastname: Choi
authors.affiliation: Research Group of Nutraceuticals for Metabolic Syndrome, Korea Food Research Institute, Seongnam 13539, Korea. ksloveda@naver.com.
authors.forename: Da-Ae
authors.initials: DA
authors.lastname: Kwon
authors.affiliation: Research Group of Metabolic Mechanism, Korea Food Research Institute, Seongnam 13539, Korea. kmj@kfri.re.kr.
authors.forename: Min Jung
authors.initials: MJ
authors.lastname: Kim
authors.affiliation: Research Group of Nutraceuticals for Metabolic Syndrome, Korea Food Research Institute, Seongnam 13539, Korea. kiseung@kfri.re.kr.
authors.forename: Ki Seung
authors.initials: KS
authors.lastname: Seong
authors.affiliation: Research Group of Nutraceuticals for Metabolic Syndrome, Korea Food Research Institute, Seongnam 13539, Korea. dhs95@kfri.re.kr.|Food Biotechnology Program, Korea University of Science and Technology, Daejeon 34113, Korea. dhs95@kfri.re.kr.
authors.forename: Dong-Hwa
authors.initials: DH
authors.lastname: Shon
mesh_terms: D029044:Achyranthes / Q000737:chemistry*; D000284:Administration, Oral; D000818:Animals; D018926:Anti-Allergic Agents / Q000008:administration & dosage / Q000494:pharmacology*; D015153:Blotting, Western; D002478:Cells, Cultured; D018925:Chemokines / Q000235:genetics / Q000378:metabolism*; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D017449:Dermatitis, Allergic Contact / Q000209:etiology / Q000378:metabolism / Q000517:prevention & control*; D005260:Female; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D010796:Phthalic Anhydrides / Q000633:toxicity*; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D053719:Tandem Mass Spectrometry; D018418:Th2 Cells / Q000187:drug effects
chemical_list: D018926:Anti-Allergic Agents; D018925:Chemokines; D016207:Cytokines; D010796:Phthalic Anhydrides; D010936:Plant Extracts; D012333:RNA, Messenger; C015559:trimellitic anhydride
keywords: Achyranthis radix; STAT6; Th2 cytokines; allergic contact dermatitis; chemokines