title: Controlling Shear Stress in 3D Bioprinting is a Key Factor to Balance Printing Resolution and Stem Cell Integrity.
abstract: A microvalve-based bioprinting system for the manufacturing of high-resolution, multimaterial 3D-structures is reported. Applying a straightforward fluid-dynamics model, the shear stress at the nozzle site can precisely be controlled. Using this system, a broad study on how cell viability and proliferation potential are affected by different levels of shear stress is conducted. Complex, multimaterial 3D structures are printed with high resolution. This work pioneers the investigation of shear stress-induced cell damage in 3D bioprinting and might help to comprehend and improve the outcome of cell-printing studies in the future.
authors.affiliation: Department of Dental Materials and Biomaterials Research, RWTH Aachen University Hospital, Pauwelsstrasse 30, 52074, Aachen, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Blaeser
authors.affiliation: Department of Dental Materials and Biomaterials Research, RWTH Aachen University Hospital, Pauwelsstrasse 30, 52074, Aachen, Germany.
authors.forename: Daniela Filipa
authors.initials: DF
authors.lastname: Duarte Campos
authors.affiliation: Department of Dental Materials and Biomaterials Research, RWTH Aachen University Hospital, Pauwelsstrasse 30, 52074, Aachen, Germany.
authors.forename: Uta
authors.initials: U
authors.lastname: Puster
authors.affiliation: Institute of Physical Chemistry, RWTH Aachen University, Landoltweg 2, 52074, Aachen, Germany.
authors.forename: Walter
authors.initials: W
authors.lastname: Richtering
authors.affiliation: Department of Materials, Department of Bioengineering and Institute of Biomedical Engineering, Imperial College London, London SW7 2AZ, London, UK.
authors.forename: Molly M
authors.initials: MM
authors.lastname: Stevens
authors.affiliation: Department of Dental Materials and Biomaterials Research, RWTH Aachen University Hospital, Pauwelsstrasse 30, 52074, Aachen, Germany.
authors.forename: Horst
authors.initials: H
authors.lastname: Fischer
mesh_terms: D062028:Bioprinting / Q000379:methods; D049109:Cell Proliferation / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D006801:Humans; D066330:Printing, Three-Dimensional; D013234:Stem Cells / Q000502:physiology*; D013314:Stress, Mechanical; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds
keywords: bioprinting; hydrogels; rheology; shear stress; stem cells