title: Detection and characterisation of Yersinia enterocolitica strains in cold-stored carcasses of large game animals in Poland.
abstract: Yersinia enterocolitica is an important foodborne pathogen. The aim of the present study was to identify the bioserotypes and virulence markers of Y.enterocolitica strains isolated from three different anatomical regions of cold-stored carcasses of large game animals intended for human consumption. Y.enterocolitica strains were found in 12/20 (60%) of the roe deer carcasses examined, 7/16 (43.8%) of red deer carcasses and 11/20 (55%) of wild boar carcasses. Of the 52 Y.enterocolitica strains, 19 were isolated from the perineum, followed by 17 strains from the peritoneum of the longissimus dorsi muscle and 16 from the tonsils. Only one strain was isolated from warm culture. Bioserotype 1A/NI was the most commonly found and was detected in 29/52 isolates. All isolates contained amplicons corresponding to ystB gene fragments. The relatively high degree of carcass contamination with Y.enterocolitica is of concern due to the growing popularity of game meat with consumers.
authors.affiliation: Department of Epizootiology, Faculty of Veterinary Medicine, University of Warmia and Mazury, Oczapowskiego 13, 10-719 Olsztyn, Poland. Electronic address: a.bancerz-kisiel@uwm.edu.pl.
authors.forename: Agata
authors.initials: A
authors.lastname: Bancerz-Kisiel
authors.affiliation: Department of Animal Reproduction with Clinic, Faculty of Veterinary Medicine, University of Warmia and Mazury, Oczapowskiego 14, 10-719 Olsztyn, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Socha
authors.affiliation: Department of Epizootiology, Faculty of Veterinary Medicine, University of Warmia and Mazury, Oczapowskiego 13, 10-719 Olsztyn, Poland.
authors.forename: Wojciech
authors.initials: W
authors.lastname: Szweda
mesh_terms: D000818:Animals; D003080:Cold Temperature; D003670:Deer; D005516:Food Microbiology; D061353:Food Storage; D008460:Meat / Q000382:microbiology*; D011044:Poland / Q000453:epidemiology; D017422:Sequence Analysis, DNA / Q000662:veterinary; D065288:Serogroup; D034421:Sus scrofa; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology / Q000382:microbiology; D015009:Yersinia Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D015008:Yersinia enterocolitica / Q000302:isolation & purification*
keywords: Cold-stored carcasses; Red deer; Roe deer; Wild boar; Yersinia enterocolitica