title: The potential effect of patulin on mice bearing melanoma cells: an anti-tumour or carcinogenic effect?
abstract: Mycotoxins are bioactive compounds that are noxious to human. Their effects on oncogenesis have been satisfactorily elucidated, and some of mycotoxins have been classified as carcinogenic to humans. Nevertheless, patulin (PAT) is considered by the International Agency of Research on Cancer as 'not carcinogenic to humans'. The present study was designed to understand the effect of this mycotoxin on melanoma cells (B16F10) by measuring cell proliferation and assessing the anti-tumour effect in vivo in Balb/c mice. Our results revealed that intraperitoneally administration of PAT for 20 days significantly induces tumour regression in B16F10 cell-implanted mice. This effect was evidenced by the activation of apoptosis which is supported by the increase in p53 and Bax expressions, the downregulation of the protein levels of Bcl2, and the increase in caspase-3 activity. Moreover, systemic toxicity analysis demonstrated that there is no potential toxicity following PAT treatment unlike untreated melanoma mice which suffer from anaemia, inflammation and liver dysfunction. Remarkably, this is the first published report demonstrating the therapeutic efficacy of PAT in vivo models.
authors.affiliation: Laboratory for Research on Biologically Compatible Compounds, Faculty of Dental Medicine, Rue Avicenne, 5019, Monastir, Tunisia.|Faculty of Sciences of Bizerte, University of Carthage, Tunis, Tunisia.
authors.forename: Manel
authors.initials: M
authors.lastname: Boussabbeh
authors.affiliation: Laboratory for Research on Biologically Compatible Compounds, Faculty of Dental Medicine, Rue Avicenne, 5019, Monastir, Tunisia.|Faculty of Sciences of Bizerte, University of Carthage, Tunis, Tunisia.
authors.forename: Intidhar
authors.initials: I
authors.lastname: Ben Salem
authors.affiliation: Laboratory for Research on Biologically Compatible Compounds, Faculty of Dental Medicine, Rue Avicenne, 5019, Monastir, Tunisia.|Faculty of Sciences of Gafsa, Gafsa, Tunisia.
authors.forename: Karima
authors.initials: K
authors.lastname: Rjiba-Touati
authors.affiliation: Laboratory for Research on Biologically Compatible Compounds, Faculty of Dental Medicine, Rue Avicenne, 5019, Monastir, Tunisia.
authors.forename: Chedy
authors.initials: C
authors.lastname: Bouyahya
authors.affiliation: Laboratory of Biochemistry-Toxicology, Monastir University Hospital, Monastir, Tunisia.
authors.forename: Fadwa
authors.initials: F
authors.lastname: Neffati
authors.affiliation: Laboratory of Biochemistry-Toxicology, Monastir University Hospital, Monastir, Tunisia.
authors.forename: Mohamed Fadhel
authors.initials: MF
authors.lastname: Najjar
authors.affiliation: Laboratory for Research on Biologically Compatible Compounds, Faculty of Dental Medicine, Rue Avicenne, 5019, Monastir, Tunisia.
authors.forename: Hassen
authors.initials: H
authors.lastname: Bacha
authors.affiliation: Laboratory for Research on Biologically Compatible Compounds, Faculty of Dental Medicine, Rue Avicenne, 5019, Monastir, Tunisia. salwaabid@yahoo.fr.
authors.forename: Salwa
authors.initials: S
authors.lastname: Abid-Essefi
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002273:Carcinogens / Q000008:administration & dosage*; D053148:Caspase 3 / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D008546:Melanoma, Experimental / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D051379:Mice; D010365:Patulin / Q000008:administration & dosage*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics; D016159:Tumor Suppressor Protein p53 / Q000235:genetics
chemical_list: D002273:Carcinogens; D019253:Proto-Oncogene Proteins c-bcl-2; D016159:Tumor Suppressor Protein p53; D010365:Patulin; D053148:Caspase 3
keywords: Anti-tumour; Apoptosis; B16F10; Melanoma; Mycotoxin; Patulin