title: A Multifunctional Frontloading Approach for Repeated Recycling of a Pressure-Controlled AFM Micropipette.
abstract: Fluid force microscopy combines the positional accuracy and force sensitivity of an atomic force microscope (AFM) with nanofluidics via a microchanneled cantilever. However, adequate loading and cleaning procedures for such AFM micropipettes are required for various application situations. Here, a new frontloading procedure is described for an AFM micropipette functioning as a force- and pressure-controlled microscale liquid dispenser. This frontloading procedure seems especially attractive when using target substances featuring high costs or low available amounts. Here, the AFM micropipette could be filled from the tip side with liquid from a previously applied droplet with a volume of only a few μL using a short low-pressure pulse. The liquid-loaded AFM micropipettes could be then applied for experiments in air or liquid environments. AFM micropipette frontloading was evaluated with the well-known organic fluorescent dye rhodamine 6G and the AlexaFluor647-labeled antibody goat anti-rat IgG as an example of a larger biological compound. After micropipette usage, specific cleaning procedures were tested. Furthermore, a storage method is described, at which the AFM micropipettes could be stored for a few hours up to several days without drying out or clogging of the microchannel. In summary, the rapid, versatile and cost-efficient frontloading and cleaning procedure for the repeated usage of a single AFM micropipette is beneficial for various application situations from specific surface modifications through to local manipulation of living cells, and provides a simplified and faster handling for already known experiments with fluid force microscopy.
authors.affiliation: Department of Physical Chemistry / Applied Laser Sensing in Complex Biosystems (ALS ComBi), Institute of Chemistry, University of Potsdam, Potsdam, Germany.
authors.forename: Phillip
authors.initials: P
authors.lastname: Roder
authors.affiliation: Department of Physical Chemistry / Applied Laser Sensing in Complex Biosystems (ALS ComBi), Institute of Chemistry, University of Potsdam, Potsdam, Germany.
authors.forename: Carsten
authors.initials: C
authors.lastname: Hille
mesh_terms: D055595:Mechanical Phenomena; D018625:Microscopy, Atomic Force / Q000295:instrumentation* / Q000379:methods; D036103:Nanotechnology / Q000379:methods; D059027:Recycling / Q000379:methods*