title: Characterization of a new subtype of allergen in dermatophagoides farinae-Der f 28.
abstract: BACKGROUND
  House dust mites (HDMs) are the major sources of indoor allergens which induce asthma, dermatitis, rhinitis, and some other allergic diseases. Close to 30 sub-allergens have been identified.
  
  
  METHODS
  Through analyzing the full genome sequence of dust mite, a new allergen whose primary structure belongs to the heat shock protein family was identified. The sequence of this allergen was determined by cDNA cloning. The allergenicity was assayed by skin prick test, Western-blot and enzyme-linked immunosorbent assay (ELISA).
  
  
  RESULTS
  r-Der f 28 bound to serum IgE from mite allergic patients. Positive responses to r-Der f 28 were shown in 11.5% by skin prick testing from 26 DM-allergic patients. Airway hyperresponsiveness, serum specific IgE and IL-4 were significantly increased in allergic asthma mouse model sensitized to r-Der f 28.
  
  
  CONCLUSIONS
  Der f 28 is a new subtype of allergen in dermatophagoides farinae.
authors.affiliation: 1 State Key Laboratory of Respiratory Disease for Allergy, School of Medicine, Shenzhen University, Shenzhen 518060,, China ; 2 State Key Laboratory of Respiratory Disease, The First Affiliated Hospital, Guangzhou Medical University, Guangzhou 510120, China.
authors.forename: Jian-Li
authors.initials: JL
authors.lastname: Lin
authors.affiliation: 1 State Key Laboratory of Respiratory Disease for Allergy, School of Medicine, Shenzhen University, Shenzhen 518060,, China ; 2 State Key Laboratory of Respiratory Disease, The First Affiliated Hospital, Guangzhou Medical University, Guangzhou 510120, China.
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Wang
authors.affiliation: 1 State Key Laboratory of Respiratory Disease for Allergy, School of Medicine, Shenzhen University, Shenzhen 518060,, China ; 2 State Key Laboratory of Respiratory Disease, The First Affiliated Hospital, Guangzhou Medical University, Guangzhou 510120, China.
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Xiao
authors.affiliation: 1 State Key Laboratory of Respiratory Disease for Allergy, School of Medicine, Shenzhen University, Shenzhen 518060,, China ; 2 State Key Laboratory of Respiratory Disease, The First Affiliated Hospital, Guangzhou Medical University, Guangzhou 510120, China.
authors.forename: Yu-Lan
authors.initials: YL
authors.lastname: Wu
authors.affiliation: 1 State Key Laboratory of Respiratory Disease for Allergy, School of Medicine, Shenzhen University, Shenzhen 518060,, China ; 2 State Key Laboratory of Respiratory Disease, The First Affiliated Hospital, Guangzhou Medical University, Guangzhou 510120, China.
authors.forename: Bao-Qing
authors.initials: BQ
authors.lastname: Sun
authors.affiliation: 1 State Key Laboratory of Respiratory Disease for Allergy, School of Medicine, Shenzhen University, Shenzhen 518060,, China ; 2 State Key Laboratory of Respiratory Disease, The First Affiliated Hospital, Guangzhou Medical University, Guangzhou 510120, China.
authors.forename: An-Jian
authors.initials: AJ
authors.lastname: Gao
authors.affiliation: 1 State Key Laboratory of Respiratory Disease for Allergy, School of Medicine, Shenzhen University, Shenzhen 518060,, China ; 2 State Key Laboratory of Respiratory Disease, The First Affiliated Hospital, Guangzhou Medical University, Guangzhou 510120, China.
authors.forename: Zhi-Gang
authors.initials: ZG
authors.lastname: Liu
authors.affiliation: 1 State Key Laboratory of Respiratory Disease for Allergy, School of Medicine, Shenzhen University, Shenzhen 518060,, China ; 2 State Key Laboratory of Respiratory Disease, The First Affiliated Hospital, Guangzhou Medical University, Guangzhou 510120, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.affiliation: 1 State Key Laboratory of Respiratory Disease for Allergy, School of Medicine, Shenzhen University, Shenzhen 518060,, China ; 2 State Key Laboratory of Respiratory Disease, The First Affiliated Hospital, Guangzhou Medical University, Guangzhou 510120, China.
authors.forename: Ping-Chang
authors.initials: PC
authors.lastname: Yang
authors.affiliation: 1 State Key Laboratory of Respiratory Disease for Allergy, School of Medicine, Shenzhen University, Shenzhen 518060,, China ; 2 State Key Laboratory of Respiratory Disease, The First Affiliated Hospital, Guangzhou Medical University, Guangzhou 510120, China.
authors.forename: Xiao-Yu
authors.initials: XY
authors.lastname: Liu
keywords: Der f 28; Dermatophagoides farinae; allergic asthma; enzyme-linked immunosorbent assay (ELISA); mouse model; skin prick test; western-blotting