title: Tumour Progression and Spontaneous Regression in the Lewis Rat Sarcoma Model.
abstract: Spontaneous regression of tumours is a fascinating phenomenon rarely observed in oncological patients. We used a Lewis rat sarcoma model in which subcutaneous tumours developed after inoculation of the R5-28/clone C4 cells. Rats with tumour progression showed splenomegaly and anaemia. Tumour growth was associated with leucocytosis, granulocytosis, decrease in lymphocyte and CD161(+) population in peripheral blood and increase in serum MCP1 concentration. Animals with spontaneous regression of tumours initially showed an increase in white blood cells number and proportion of granulocytes. Between the 42nd and 49th day, however, values of these parameters dropped in correlation with reduction of tumour size. In spontaneously regressed tumours, vascularization was higher and on the contrary, progressive tumours had more necrotic areas with a high number of infiltrating granulocytes. In conclusion, progression and spontaneous regression of tumours in the Lewis rat sarcoma model is associated with distinct changes in populations of blood cells and immune cells which participate in these completely different processes of tumourigenesis.
authors.affiliation: Institute of Animal Physiology and Genetics, Liběchov, Czech Republic Department of Husbandry and Ethology of Animals, Czech University of Life Sciences, Prague, Czech Republic.
authors.forename: Jana
authors.initials: J
authors.lastname: Kovalská
authors.affiliation: Institute of Animal Physiology and Genetics, Liběchov, Czech Republic Veterinary Sciences, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences, Prague, Czech Republic.
authors.forename: Rajbardhan
authors.initials: R
authors.lastname: Mishra
authors.affiliation: Department of Husbandry and Ethology of Animals, Czech University of Life Sciences, Prague, Czech Republic.
authors.forename: Lukáš
authors.initials: L
authors.lastname: Jebavý
authors.affiliation: Department of Transgenic Models of Diseases, Institute of Molecular Genetics, Czech Centre for Phenogenomics, BIOCEV, Prague, Czech Republic.
authors.forename: Peter
authors.initials: P
authors.lastname: Makovický
authors.affiliation: Institute of Animal Physiology and Genetics, Liběchov, Czech Republic.
authors.forename: Josef
authors.initials: J
authors.lastname: Janda
authors.affiliation: Department of Immunology, Third Faculty of Medicine, Charles University in Prague, Prague, Czech Republic Department of Immunology, Faculty of Science, Charles University in Prague, Prague, Czech Republic.
authors.forename: Daniela
authors.initials: D
authors.lastname: Plánská
authors.affiliation: Institute of Animal Physiology and Genetics, Liběchov, Czech Republic Department of Surgery, First Medical Faculty of Charles University, Na Bulovce Hospital, Prague, Czech Republic.
authors.forename: Monika
authors.initials: M
authors.lastname: Červinková
authors.affiliation: Institute of Animal Physiology and Genetics, Liběchov, Czech Republic.
authors.forename: Vratislav
authors.initials: V
authors.lastname: Horák
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D018450:Disease Progression; D005260:Female; D007150:Immunohistochemistry; D007694:Killer Cells, Natural / Q000378:metabolism*; D051381:Rats; D011917:Rats, Inbred Lew; D012509:Sarcoma / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology
keywords: MCP1; Spontaneous regression; natural killer cells; platelets; progression; sarcoma