title: Rush Oral Immunotherapy Does Not Reduce Allergic Response in Mice with Mild Allergy to Egg White Ovomucoid.
abstract: Oral immunotherapy (OIT) is a promising therapeutic approach for treating food allergy. Past studies have shown that OIT reduces allergic response only in severe allergy model mice. We worked to establish mild allergy model mice, and investigated whether 'rush' OIT for 10 d improved the allergic response and biomarkers in these mice. Balb/c mice were sensitized to ovomucoid (OM) in alum. The rush OIT was done for 10 d. Oral OM challenge was used to determine the impact of OIT on the allergic response. We measured allergic biomarkers, such as vascular permeability in the skin, plasma levels of total IgE, OM-specific IgE, IgG1 and IgG2a and cytokines in splenocyte culture supernatant. OIT for 10 d did not improve allergy symptoms and increased vascular permeability. Total IgE in the plasma of OIT-treated mice was significantly higher than in that of non-treated mice. OM-specific IgG1 and IgG2a plasma levels were not significantly different between OIT-treated and non-treated mice. Among the cytokine secretion of splenocyte from OIT-treated mice, IFN-γ and IL-10 were significantly lower than in non-treated mice, and IL-4 and IL-5 were significantly higher. Total TGF-β in the OIT-treated group was not detected. The IFN-γ/IL-4 ratio of the OIT-treated group was about 1/8 that of the non-treated group. OIT for 10 d was not effective and some biomarkers showed negative responses in the mild allergy model mice. We suggest OIT should be used very carefully as this treatment carries a risk of worsening allergy symptoms for mice with mild allergy.
authors.affiliation: Department of Food Science and Nutrition, School of Human Environmental Sciences, Mukogawa Women's University.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Maeta
authors.forename: Mayuko
authors.initials: M
authors.lastname: Kaji
authors.forename: Minami
authors.initials: M
authors.lastname: Nagaishi
authors.forename: Aoi
authors.initials: A
authors.lastname: Hirakawa
authors.forename: Kyoko
authors.initials: K
authors.lastname: Takahashi
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood; D002478:Cells, Cultured; D004195:Disease Models, Animal; D021181:Egg Hypersensitivity / Q000097:blood / Q000188:drug therapy / Q000276:immunology*; D007073:Immunoglobulin E / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D007167:Immunotherapy / Q000379:methods*; D007371:Interferon-gamma / Q000097:blood; D016753:Interleukin-10 / Q000097:blood; D015847:Interleukin-4 / Q000097:blood; D015848:Interleukin-5 / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D010059:Ovomucin / Q000009:adverse effects* / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000097:blood
chemical_list: D015415:Biomarkers; D007074:Immunoglobulin G; D015848:Interleukin-5; D016212:Transforming Growth Factor beta; D016753:Interleukin-10; D015847:Interleukin-4; D010059:Ovomucin; D007073:Immunoglobulin E; D007371:Interferon-gamma