title: IL-10 derived from CD1dhiCD5⁺ B cells regulates experimental autoimmune myasthenia gravis.
abstract: IL-10-competent subset within CD1d(hi)CD5(+) B cells, also known as B10 cells, has been shown to regulate autoimmune diseases. In our previous study, adoptive transfer of CD1d(hi)CD5(+) B cells expanded in vivo by GM-CSF prevented and suppressed experimental autoimmune myasthenia gravis (EAMG). The goal of this study was to further examine the role and mechanism of IL-10 in the regulatory function of B10 cells in EAMG. We found that only IL-10 competent CD1d(hi)CD5(+) B cells sorted from WT mice, but not IL-10 deficient CD1d(hi)CD5(+) B cells exhibited regulatory function in vitro and in vivo. Adoptive transfer of IL-10 competent CD1d(hi)CD5(+) B cells led to higher frequency of Tregs and B10 cells, and low levels of proinflammatory cytokines and autoantibody production. We conclude that IL-10 production within CD1d(hi)CD5(+) B cells plays an important role in immune regulation of EAMG.
authors.affiliation: Department of Neurology, University of Chicago, Chicago, IL 60637, USA. Electronic address: sheng1@uchicago.edu.
authors.forename: Jian Rong
authors.initials: JR
authors.lastname: Sheng
authors.affiliation: Department of Neurology, University of Chicago, Chicago, IL 60637, USA.
authors.forename: Songhua
authors.initials: S
authors.lastname: Quan
authors.affiliation: Department of Neurology, University of Chicago, Chicago, IL 60637, USA.
authors.forename: Betty
authors.initials: B
authors.lastname: Soliven
mesh_terms: D019264:Adoptive Transfer / Q000379:methods; D000818:Animals; D055610:Antigens, CD1d / Q000378:metabolism*; D016175:B-Lymphocyte Subsets / Q000737:chemistry*; D018956:CD5 Antigens / Q000378:metabolism*; D049109:Cell Proliferation / Q000502:physiology; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D005434:Flow Cytometry; D016753:Interleukin-10 / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D018482:Muscle, Skeletal / Q000378:metabolism; D020720:Myasthenia Gravis, Autoimmune, Experimental / Q000139:chemically induced / Q000276:immunology* / Q000473:pathology* / Q000503:physiopathology; D010446:Peptide Fragments / Q000276:immunology; D011978:Receptors, Nicotinic / Q000276:immunology
chemical_list: D055610:Antigens, CD1d; C526877:CD1D protein, human; D018956:CD5 Antigens; D016207:Cytokines; D010446:Peptide Fragments; D011978:Receptors, Nicotinic; C041736:Torpedo acetylcholine receptor, alpha-subunit 1-20; D016753:Interleukin-10
keywords: Acetylcholine receptor; Autoimmune disease; Cytokines; Immune tolerance; Regulatory B cells; Tregs