title: Prevention of Dielectric Breakdown of Nanopore Membranes by Charge Neutralization.
abstract: To achieve DNA sequencing using a solid-state nanopore, it is necessary to reduce the electric noise current. The noise current can be decreased by reducing the capacitance (C) of the nanopore device. However, we found that an electric-charge difference (ΔQ) between the electrolyte in one chamber and the electrolyte in another chamber occurred. For low capacitance devices, this electric-charge imbalance can lead to unexpectedly high voltage (ΔV = ΔQ/C) which disrupted the membrane when the two electrolytes were independently poured into the chambers. We elucidated the mechanism for the generation of initial defects and established new procedures for preventing the generation of defects by connecting an electric bypass between the chambers.
authors.affiliation: Hitachi, Ltd., Research &Development Group, Center for Technology Innovation - Healthcare, 1-280 Higashi-koigakubo, Kokubunji, Tokyo, 185-8603.
authors.forename: Kazuma
authors.initials: K
authors.lastname: Matsui
authors.affiliation: Hitachi, Ltd., Research &Development Group, Center for Technology Innovation - Healthcare, 1-280 Higashi-koigakubo, Kokubunji, Tokyo, 185-8603.
authors.forename: Itaru
authors.initials: I
authors.lastname: Yanagi
authors.affiliation: Hitachi, Ltd., Research &Development Group, Center for Technology Innovation - Healthcare, 1-280 Higashi-koigakubo, Kokubunji, Tokyo, 185-8603.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Goto
authors.affiliation: Hitachi, Ltd., Research &Development Group, Center for Technology Innovation - Healthcare, 1-280 Higashi-koigakubo, Kokubunji, Tokyo, 185-8603.
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Takeda
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D025802:Electric Capacitance; D008567:Membranes, Artificial; D058608:Nanopores; D036103:Nanotechnology / Q000379:methods*; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D008567:Membranes, Artificial; D004247:DNA