title: Cadmium sulfide nanoparticles trigger DNA alterations and modify the bioturbation activity of tubificidae worms exposed through the sediment.
abstract: To address the impact of cadmium sulfide nanoparticles (CdS NPs) in freshwater ecosystems, aquatic oligochaete Tubifex tubifex were exposed through the sediment to a low dose (0.52 mg of 8 nm in size of CdS NPs/kg) for 20 days using microcosms. Cadmium (Cd) was released from the CdS NPs-contaminated sediment to the water column, and during this period the average concentrations of Cd in the filtered water fraction were 0.026 ± 0.006 µg/L in presence of oligochaetes. Similar experiments with microparticular CdS and cadmium chloride (CdCl2) were simultaneously performed for comparative purposes. CdS NPs exposure triggered various effects on Tubifex worms compared to control, microsized and ionic reference, including modification of genome composition as assessed using RAPD-PCR genotoxicity tests. Bioaccumulation levels showed that CdS NPs were less bioavailable than CdCl2 to oligochaetes and reached 0.08 ± 0.01 µg Cd/g for CdS NPs exposure versus 0.76 ± 0.3 µg Cd/g for CdCl2 exposure (fresh weight). CdS NPs altered worm's behavior by decreasing significantly the bioturbation activity as assessed after the exposure period using conservative fluorescent particulate tracers. This study demonstrated the high potential harm of the CdS nanoparticular form despite its lower bioavailability for Tubifex worms.
authors.affiliation: a University of Bordeaux, CNRS, UMR EPOC 5805, Arcachon Marine Station, Place du Dr Peyneau , 33120 Arcachon , France .
authors.forename: Amina
authors.initials: A
authors.lastname: Dedeh
authors.affiliation: a University of Bordeaux, CNRS, UMR EPOC 5805, Arcachon Marine Station, Place du Dr Peyneau , 33120 Arcachon , France .
authors.forename: Aurélie
authors.initials: A
authors.lastname: Ciutat
authors.affiliation: b University of Bordeaux, CNRS, UMR EPOC 5805, Bâtiment B18, Allée Geoffroy Saint-Hilaire , 33615 Pessac , France .
authors.forename: Pascal
authors.initials: P
authors.lastname: Lecroart
authors.affiliation: c University of Bordeaux, CNRS, UPR 9048, Institute of Chemistry of Condensed Matter of Bordeaux, 87, Avenue du Docteur Schweitzer , 33600 Pessac , France , and.
authors.forename: Mona
authors.initials: M
authors.lastname: Treguer-Delapierre
authors.affiliation: a University of Bordeaux, CNRS, UMR EPOC 5805, Arcachon Marine Station, Place du Dr Peyneau , 33120 Arcachon , France .|d CRIIGEN , Paris , France.
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Bourdineaud
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects*; D001682:Biological Availability; D002104:Cadmium / Q000032:analysis; D019256:Cadmium Chloride / Q000493:pharmacokinetics / Q000633:toxicity; D019187:Cadmium Compounds / Q000737:chemistry / Q000493:pharmacokinetics / Q000633:toxicity*; D004247:DNA / Q000187:drug effects* / Q000235:genetics; D017753:Ecosystem; D054750:Ecotoxicology; D005618:Fresh Water / Q000737:chemistry; D019015:Geologic Sediments / Q000737:chemistry*; D016296:Mutagenesis / Q000187:drug effects; D053758:Nanoparticles / Q000737:chemistry / Q000633:toxicity*; D009835:Oligochaeta / Q000187:drug effects* / Q000235:genetics*; D019105:Random Amplified Polymorphic DNA Technique; D013440:Sulfides / Q000737:chemistry / Q000493:pharmacokinetics / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000737:chemistry / Q000633:toxicity*
chemical_list: D019187:Cadmium Compounds; D013440:Sulfides; D014874:Water Pollutants, Chemical; D002104:Cadmium; C034939:cadmium sulfide; D004247:DNA; D019256:Cadmium Chloride
keywords: Bioturbation activity; cadmium; genotoxicity; nanoparticles; tubificidae