title: Prognostic significance of peripheral monocytic myeloid-derived suppressor cells and monocytes in patients newly diagnosed with diffuse large b-cell lymphoma.
abstract: OBJECTIVE
  The revised International Prognostic Index (R-IPI) aids in predicting the prognosis of patients with diffuse large B cell lymphoma (DLBCL), but R-IPI yields no significant differences in assessing different subtypes of DLBCL. It is necessary to identify patients with a high-risk of DLBCL and alternative therapy should be delivered as early as possible.
  
  
  METHODS
  In total, 144 patients newly diagnosed with DLBCL including 63 GCB-DLBCL and 81 non-GCB-DLBCL and 30 healthy controls were enrolled. Peripheral monocytic myeloid-derived suppressor cells (M-MDSC) (CD14(+)HLA(-)DR(low/-)) were detected by flow cytometry and the percentage of monocytes (MΦ) was evaluated by completed blood count (CBC). The correlation between M-MDSC% and MΦ% was statistically analyzed.
  
  
  RESULTS
  Compared with healthy controls, significant increase was observed in M-MDSC% and MΦ% in DLBCL patients (both P<0.001). Significant difference of M-MDSC% was found between GCB-DLBCL and non-GCB-DLBCL patients in both poor (P<0.001) and very good groups (P=0.03), whereas no statistical significance in the good group (P>0.05). The MΦ% in non-GCB-DLBCL patients was significantly higher than that in GCB-DLBCL counterparts merely in the poor group (P<0.001). Positive correlation was noted between MΦ% and M-MDSC in all DLBCL patients rather than in healthy controls (r=0.227 P=0.229).
  
  
  CONCLUSIONS
  The percentage of peripheral MΦ was positively correlated with M-MDSC% in patients with different subtypes and risks of DLBCL. Peripheral MΦ% and M-MDSC% combined with R-IPI score may be useful for predicting the prognosis of patients newly-diagnosed with DLBCL.
authors.affiliation: Department of Hematology, The Second Hospital of Lanzhou University Lanzhou 730030, Gansu Province, P R. China.
authors.forename: Chongyang
authors.initials: C
authors.lastname: Wu
authors.affiliation: Department of Cardiac Surgery, The Second Hospital of Lanzhou University Lanzhou 730030, Gansu Province, P R. China.
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Wu
authors.affiliation: Department of Pediatric, The First Hospital of Lanzhou Lanzhou 730050, Gansu Province, P R. China.
authors.forename: Xiaoni
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Hematology, The Second Hospital of Lanzhou University Lanzhou 730030, Gansu Province, P R. China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Chai
authors.affiliation: Institute of Urology, The Second Hospital of Lanzhou University Lanzhou 730030, Gansu Province, P R. China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Guo
authors.affiliation: Department of Hematology, The Second Hospital of Lanzhou University Lanzhou 730030, Gansu Province, P R. China.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Hematology, The Second Hospital of Lanzhou University Lanzhou 730030, Gansu Province, P R. China.
authors.forename: Lingling
authors.initials: L
authors.lastname: Yue
authors.affiliation: Institute of Hematology, The Second Hospital of Lanzhou University Lanzhou 730030, Gansu Province, P R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Bai
authors.affiliation: Institute of Urology, Key Laboratory of Urological Diseases in Gansu Province, Gansu Nephro-Urological Clinical Center, The Second Hospital of Lanzhou University Lanzhou 730030, Gansu Province, P R. China.
authors.forename: Zhiping
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Hematology, The Second Hospital of Lanzhou University Lanzhou 730030, Gansu Province, P R. China.
authors.forename: Liansheng
authors.initials: L
authors.lastname: Zhang
keywords: Monocytic myeloid-derived suppressor cell; diffuse large B-cell lymphoma; monocyte; revised international prognostic index