title: Design and synthesis of environmentally sensitive fluorescent 2-naphthylethynylated 2'-deoxyadenosines: Detection of target DNA via changes in fluorescence intensity and wavelength.
abstract: Various C2-naphthylethynylated 2'-deoxyadenosines were synthesized as environmentally sensitive fluorescent (ESF) nucleosides and their photophysical properties were examined. Among the ESF nucleosides synthesized, four exhibited strong solvatochromicity, two of which were incorporated into oligodeoxynucleotides (ODNs). These ODN probes were able to detect target DNA through distinct changes in fluorescence intensity and wavelength and acted as effective reporter probes.
authors.affiliation: Department of Chemical Biology and Applied Chemistry, College of Engineering, Nihon University, Koriyama, Fukushima 963-8642, Japan.
authors.forename: Azusa
authors.initials: A
authors.lastname: Suzuki
authors.affiliation: Department of Chemical Biology and Applied Chemistry, College of Engineering, Nihon University, Koriyama, Fukushima 963-8642, Japan.
authors.forename: Ryota
authors.initials: R
authors.lastname: Kozakai
authors.affiliation: Department of Chemical Biology and Applied Chemistry, College of Engineering, Nihon University, Koriyama, Fukushima 963-8642, Japan.
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Aso
authors.affiliation: Department of Chemical Biology and Applied Chemistry, College of Engineering, Nihon University, Koriyama, Fukushima 963-8642, Japan; Institute of Advanced Energy, Kyoto University, Uji, Kyoto 611-0011, Japan.
authors.forename: Isao
authors.initials: I
authors.lastname: Saito
authors.affiliation: Department of Chemical Biology and Applied Chemistry, College of Engineering, Nihon University, Koriyama, Fukushima 963-8642, Japan.
authors.forename: Takuya
authors.initials: T
authors.lastname: Takeda
authors.affiliation: Department of Chemical Biology and Applied Chemistry, College of Engineering, Nihon University, Koriyama, Fukushima 963-8642, Japan.
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Saito
mesh_terms: D004247:DNA / Q000032:analysis*; D003839:Deoxyadenosines / Q000138:chemical synthesis / Q000737:chemistry*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D003839:Deoxyadenosines; D005456:Fluorescent Dyes; D004247:DNA; C058118:2'-deoxyadenosine
keywords: DNA; Fluorescent probe; Nucleoside