title: Influence of Sorghum Kafirin on Serum Lipid Profile and Antioxidant Activity in Hyperlipidemic Rats (In Vitro and In Vivo Studies).
abstract: The aim of this study was to compare in vitro the antioxidant potential of sorghum kafirin and sorghum flour and their influence on lipids and antioxidant capacity in rats. The antioxidant activity in sorghum kafirin extract measured by the DPPH and TEAC methods was increased 30 and 65 times, respectively, compared to that of its counterpart, sorghum flour. According to electrophoresis assay, the kafirins tert-butanol extract showed a high proportion of α-kafirin monomers, and its amino acid composition revealed higher hydrophobic amino acid content such as alanine, isoleucine, leucine, tyrosine and phenylalanine than sorghum flour extract. Diets supplemented with sorghum kafirin extract have improved lipid metabolism and increased the serum antioxidant potential (67%) especially in rats fed with added cholesterol. The bioactive peptides generated from kafirin in vivo hydrolysis appear to be associated with the positive effect on serum lipids and antioxidant activity. According to these results, sorghum kafirin extract at the levels used in this study apparently could be used for prevention of atherosclerosis and other chronic diseases.
authors.affiliation: Departamento de Investigación y Posgrado en Alimentos, Universidad de Sonora, Boulevard Luis Encinas y Rosales, Colonia Centro, 83000 Hermosillo Sonora, SON, Mexico.
authors.forename: Raquel A
authors.initials: RA
authors.lastname: Ortíz Cruz
authors.affiliation: Departamento de Investigación y Posgrado en Alimentos, Universidad de Sonora, Boulevard Luis Encinas y Rosales, Colonia Centro, 83000 Hermosillo Sonora, SON, Mexico.
authors.forename: José L
authors.initials: JL
authors.lastname: Cárdenas López
authors.affiliation: Centro de Investigación en Alimentación y Desarrollo, A.C. (CIAD, A.C.), Carretera a La Victoria, Km 0.6, 83304 Hermosillo Sonora, SON, Mexico.
authors.forename: Gustavo A
authors.initials: GA
authors.lastname: González Aguilar
authors.affiliation: Centro de Investigación en Alimentación y Desarrollo, A.C. (CIAD, A.C.), Carretera a La Victoria, Km 0.6, 83304 Hermosillo Sonora, SON, Mexico.
authors.forename: Humberto
authors.initials: H
authors.lastname: Astiazarán García
authors.affiliation: Institute for Drug Research, School of Pharmacy, The Hebrew University of Jerusalem, P.O. Box 12065, 91120 Jerusalem, Israel.
authors.forename: Shela
authors.initials: S
authors.lastname: Gorinstein
authors.affiliation: Departamento de Investigación y Posgrado en Alimentos, Universidad de Sonora, Boulevard Luis Encinas y Rosales, Colonia Centro, 83000 Hermosillo Sonora, SON, Mexico.
authors.forename: Rafael
authors.initials: R
authors.lastname: Canett Romero
authors.affiliation: Departamento de Investigación y Posgrado en Alimentos, Universidad de Sonora, Boulevard Luis Encinas y Rosales, Colonia Centro, 83000 Hermosillo Sonora, SON, Mexico.
authors.forename: Maribel
authors.initials: M
authors.lastname: Robles Sánchez
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D019587:Dietary Supplements; D004305:Dose-Response Relationship, Drug; D005433:Flour; D006949:Hyperlipidemias / Q000175:diagnosis / Q000178:diet therapy* / Q000378:metabolism*; D008055:Lipids / Q000097:blood*; D010940:Plant Proteins / Q000008:administration & dosage*; D051381:Rats; D017208:Rats, Wistar; D016896:Treatment Outcome
chemical_list: D000975:Antioxidants; D008055:Lipids; D010940:Plant Proteins; C080615:kafirin protein, Sorghum bicolor