title: Expanded endothelial progenitor cells mitigate lung injury in septic mice.
abstract: Endothelial progenitor cells (EPCs) improve survival and reduce organ failure in cecal ligation and puncture-induced sepsis; however, expanded EPCs may represent an even better approach for vascular repair. To date, no study has compared the effects of non-expanded EPCs (EPC-NEXP) with those of expanded EPCs (EPC-EXP) and mesenchymal stromal cells of human (MSC-HUMAN) and mouse (MSC-MICE) origin in experimental sepsis. One day after cecal ligation and puncture sepsis induction, BALB/c mice were randomized to receive saline, EPC-EXP, EPC-NEXP, MSC-HUMAN or MSC-MICE (1 × 10(5)) intravenously. EPC-EXP, EPC-NEXP, MSC-HUMAN, and MSC-MICE displayed differences in phenotypic characterization. On days 1 and 3, cecal ligation and puncture mice showed decreased survival rate, and increased elastance, diffuse alveolar damage, and levels of interleukin (IL)-1β, IL-6, IL-10, tumor necrosis factor-α, vascular endothelial growth factor, and platelet-derived growth factor in lung tissue. EPC-EXP and MSC-HUMAN had reduced elastance, diffuse alveolar damage, and platelet-derived growth factor compared to no-cell treatment. Tumor necrosis factor-α levels decreased in the EPC-EXP, MSC-HUMAN, and MSC-MICE groups. IL-1β levels decreased in the EPC-EXP group, while IL-10 decreased in the MSC-MICE. IL-6 levels decreased both in the EPC-EXP and MSC-MICE groups. Vascular endothelial growth factor levels were reduced regardless of therapy. In conclusion, EPC-EXP and MSC-HUMAN yielded better lung function and reduced histologic damage in septic mice.
authors.affiliation: Department of Anesthesiology and Intensive Care Medicine, University Hospital Dresden, Technische Universität Dresden, Dresden, Germany. Andreas.Gueldner@uniklinikum-dresden.de.|Laboratory of Pulmonary Investigation, Health Sciences Center, Carlos Chagas Filho Biophysics Institute, Universidade Federal do Rio de Janeiro, Ilha do Fundão, Rio de Janeiro, Brazil. Andreas.Gueldner@uniklinikum-dresden.de.
authors.forename: Andreas
authors.initials: A
authors.lastname: Güldner
authors.affiliation: Laboratory of Pulmonary Investigation, Health Sciences Center, Carlos Chagas Filho Biophysics Institute, Universidade Federal do Rio de Janeiro, Ilha do Fundão, Rio de Janeiro, Brazil. tati.maron@gmail.com.|Laboratory of Immunopharmacology, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil. tati.maron@gmail.com.
authors.forename: Tatiana
authors.initials: T
authors.lastname: Maron-Gutierrez
authors.affiliation: Laboratory of Pulmonary Investigation, Health Sciences Center, Carlos Chagas Filho Biophysics Institute, Universidade Federal do Rio de Janeiro, Ilha do Fundão, Rio de Janeiro, Brazil. soraiafisio@gmail.com.
authors.forename: Soraia Carvalho
authors.initials: SC
authors.lastname: Abreu
authors.affiliation: Laboratory of Pulmonary Investigation, Health Sciences Center, Carlos Chagas Filho Biophysics Institute, Universidade Federal do Rio de Janeiro, Ilha do Fundão, Rio de Janeiro, Brazil. deboraxisto@gmail.com.
authors.forename: Debora Gonçalves
authors.initials: DG
authors.lastname: Xisto
authors.affiliation: Center for Cell Technology, Pontifícia Universidade Católica do Paraná, Curitiba, Paraná, Brazil. alexandra.senegaglia@pucpr.br.
authors.forename: Alexandra Cristina
authors.initials: AC
authors.lastname: Senegaglia
authors.affiliation: Laboratory of Pulmonary Investigation, Health Sciences Center, Carlos Chagas Filho Biophysics Institute, Universidade Federal do Rio de Janeiro, Ilha do Fundão, Rio de Janeiro, Brazil. patty.barcelos@gmail.com.
authors.forename: Patty Rose da Silva
authors.initials: PR
authors.lastname: Barcelos
authors.affiliation: Laboratory of Pulmonary Investigation, Health Sciences Center, Carlos Chagas Filho Biophysics Institute, Universidade Federal do Rio de Janeiro, Ilha do Fundão, Rio de Janeiro, Brazil. johnatasdutra@gmail.com.
authors.forename: Johnatas Dutra
authors.initials: JD
authors.lastname: Silva
authors.affiliation: Center for Cell Technology, Pontifícia Universidade Católica do Paraná, Curitiba, Paraná, Brazil. paulo.brofman@pucpr.br.
authors.forename: Paulo
authors.initials: P
authors.lastname: Brofman
authors.affiliation: Department of Anesthesiology and Intensive Care Medicine, University Hospital Dresden, Technische Universität Dresden, Dresden, Germany. mgabreu@uniklinikum-dresden.de.
authors.forename: Marcelo Gama
authors.initials: MG
authors.lastname: de Abreu
authors.affiliation: Laboratory of Pulmonary Investigation, Health Sciences Center, Carlos Chagas Filho Biophysics Institute, Universidade Federal do Rio de Janeiro, Ilha do Fundão, Rio de Janeiro, Brazil. prmrocco@biof.ufrj.br.
authors.forename: Patricia Rieken Macedo
authors.initials: PR
authors.lastname: Rocco
mesh_terms: D000071916:AC133 Antigen; D000818:Animals; D015703:Antigens, CD; D049109:Cell Proliferation; D066026:Endothelial Progenitor Cells; D005312:Fetal Blood; D006023:Glycoproteins; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D008168:Lung / Q000378:metabolism / Q000473:pathology; D055370:Lung Injury / Q000209:etiology / Q000473:pathology / Q000628:therapy*; D051379:Mice; D008807:Mice, Inbred BALB C; D010455:Peptides; D010641:Phenotype; D012129:Respiratory Function Tests; D018805:Sepsis / Q000150:complications*
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D006023:Glycoproteins; D018836:Inflammation Mediators; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides