title: Resveratrol relieves ischemia-induced oxidative stress in the hippocampus by activating SIRT1.
abstract: Resveratrol, a naturally occurring phytoalexin, acts as an activator of sirtuin 1 (SIRT1) and has been shown to have a neuroprotective role in various models. Healthy adult male Sprague-Dawley rats were subjected to cerebral ischemia in order to study the protective effect of resveratrol on the brain following ischemia, and to investigate the effects of SIRT1 activation on the hippocampus. Untreated and resveratrol-treated rats were anesthetized prior to undergoing surgery to induce middle cerebral artery occlusion followed by reperfusion. SIRT1 expression was evaluated by immunohistochemistry, western blotting and reverse transcription-quantitative polymerase chain reaction, and SIRT1 activity was also evaluated. In addition, terminal deoxynucleotidyl transferase dUTP nick end-labeling (TUNEL) and Nissl staining assays were conducted and the levels of reactive oxygen species were determined. It was observed that resveratrol significantly decreased the number of TUNEL-positive cells and increased the expression of SIRT1 mRNA in a dose-dependent manner. This was accompanied by increases in SIRT1 protein expression levels and SIRT1 activity. The results demonstrate the neuroprotective and antioxidant effects of resveratrol against ischemia-induced apoptosis in the rat hippocampus.
authors.affiliation: Department of Human Anatomy, The School of Medicine of Inner Mongolia University for The Nationalities, Tongliao, Inner Mongolia 028041, P.R. China ; Department of Preventive Medicine, The School of Medicine of Inner Mongolia University for The Nationalities, Tongliao, Inner Mongolia 028041, P.R. China.
authors.forename: Zhuangzhi
authors.initials: Z
authors.lastname: Meng
authors.affiliation: Department of Human Anatomy, The School of Medicine of Inner Mongolia University for The Nationalities, Tongliao, Inner Mongolia 028041, P.R. China ; Department of Preventive Medicine, The School of Medicine of Inner Mongolia University for The Nationalities, Tongliao, Inner Mongolia 028041, P.R. China ; Laboratory of Biomedicine and Department of Mongolian Medicine Hematology-Oncology, The Affiliated Hospital of Inner Mongolia University for The Nationalities, Tongliao, Inner Mongolia 028007, P.R. China.
authors.forename: Jianguo
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Human Anatomy, The School of Medicine of Inner Mongolia University for The Nationalities, Tongliao, Inner Mongolia 028041, P.R. China ; Department of Preventive Medicine, The School of Medicine of Inner Mongolia University for The Nationalities, Tongliao, Inner Mongolia 028041, P.R. China.
authors.forename: Honglin
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Department of Human Anatomy, The School of Medicine of Inner Mongolia University for The Nationalities, Tongliao, Inner Mongolia 028041, P.R. China ; Department of Preventive Medicine, The School of Medicine of Inner Mongolia University for The Nationalities, Tongliao, Inner Mongolia 028041, P.R. China.
authors.forename: Haiying
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Human Anatomy, The School of Medicine of Inner Mongolia University for The Nationalities, Tongliao, Inner Mongolia 028041, P.R. China ; Department of Preventive Medicine, The School of Medicine of Inner Mongolia University for The Nationalities, Tongliao, Inner Mongolia 028041, P.R. China.
authors.forename: Guowei
authors.initials: G
authors.lastname: Zhang
authors.affiliation: Department of Human Anatomy, The School of Medicine of Inner Mongolia University for The Nationalities, Tongliao, Inner Mongolia 028041, P.R. China ; Department of Preventive Medicine, The School of Medicine of Inner Mongolia University for The Nationalities, Tongliao, Inner Mongolia 028041, P.R. China.
authors.forename: Lingzhan
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Human Anatomy, The School of Medicine of Inner Mongolia University for The Nationalities, Tongliao, Inner Mongolia 028041, P.R. China ; Department of Preventive Medicine, The School of Medicine of Inner Mongolia University for The Nationalities, Tongliao, Inner Mongolia 028041, P.R. China.
authors.forename: H E
authors.initials: HE
authors.lastname: Hu
authors.affiliation: Department of Human Anatomy, The School of Medicine of Inner Mongolia University for The Nationalities, Tongliao, Inner Mongolia 028041, P.R. China ; Department of Preventive Medicine, The School of Medicine of Inner Mongolia University for The Nationalities, Tongliao, Inner Mongolia 028041, P.R. China.
authors.forename: D I
authors.initials: DI
authors.lastname: Li
authors.affiliation: Department of Human Anatomy, The School of Medicine of Inner Mongolia University for The Nationalities, Tongliao, Inner Mongolia 028041, P.R. China ; Department of Preventive Medicine, The School of Medicine of Inner Mongolia University for The Nationalities, Tongliao, Inner Mongolia 028041, P.R. China.
authors.forename: Mingjing
authors.initials: M
authors.lastname: Liu
authors.affiliation: Department of Human Anatomy, The School of Medicine of Inner Mongolia University for The Nationalities, Tongliao, Inner Mongolia 028041, P.R. China ; Department of Preventive Medicine, The School of Medicine of Inner Mongolia University for The Nationalities, Tongliao, Inner Mongolia 028041, P.R. China.
authors.forename: Fulong
authors.initials: F
authors.lastname: Bi
authors.affiliation: Department of Human Anatomy, The School of Medicine of Inner Mongolia University for The Nationalities, Tongliao, Inner Mongolia 028041, P.R. China ; Department of Preventive Medicine, The School of Medicine of Inner Mongolia University for The Nationalities, Tongliao, Inner Mongolia 028041, P.R. China.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Human Anatomy, The School of Medicine of Inner Mongolia University for The Nationalities, Tongliao, Inner Mongolia 028041, P.R. China ; Department of Preventive Medicine, The School of Medicine of Inner Mongolia University for The Nationalities, Tongliao, Inner Mongolia 028041, P.R. China.
authors.forename: Geng
authors.initials: G
authors.lastname: Tian
authors.affiliation: Tianjin Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Science and Peking Union Medical College, Tianjin 300192, P.R. China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Laboratory of Biomedicine and Department of Mongolian Medicine Hematology-Oncology, The Affiliated Hospital of Inner Mongolia University for The Nationalities, Tongliao, Inner Mongolia 028007, P.R. China.
authors.forename: Batu
authors.initials: B
authors.lastname: Buren
keywords: brain ischemia; hippocampus; reactive oxygen species; resveratrol; sirtuin 1