title: MiR-338-3p inhibits the proliferation and migration of gastric cancer cells by targeting ADAM17.
abstract: BACKGROUND
  MicroRNAs (miRNA) have been documented playing a critical role in cancer progression. Although miR-338-3p has been implicated in several cancers, its role in gastric cancer is still unknown. The aim of our study was to investigate the role of miR-338-3p in gastric cancer progression.
  
  
  METHODS
  Expression levels of miR-338-3p in gastric cancer cell lines and tissues were determined by quantitative real-time PCR (qRT-PCR). The effect of miR-338-3p on proliferation was evaluated by MTT assay, cell migration and invasion were evaluated by transwell migration and invasion assays. Furthermore, luciferase reporter assay was conducted to confirm the target gene of miR-338-3p, and the results were validated in gastric cancer cells.
  
  
  RESULTS
  In the present study, we found that miR-338-3p was down-regulated in both gastric cancer cell lines and tissues. Enforced expression of miR-338-3p inhibited proliferation, migration and invasion of gastric cancer cells in vitro. Moreover, we identified A disintegrin and metalloproteinase 17 (ADAM17) gene as potential target of miR-338-3p. Importantly, ADAM17 rescued the miR-338-3p mediated inhibition of cell proliferation, migration and invasion.
  
  
  CONCLUSIONS
  Our study suggested that miR-338-3p is significantly decreased in gastric cancer, and inhibits cell proliferation, migration and invasion partially via the downregulation of ADAM17. Thus, miR-338-3p may represent a potential therapeutic target for gastric cancer intervention.
authors.affiliation: Department of General Surgery, Huaihe Hospital of HeNan University Kaifeng 475000, Henan Province, China.
authors.forename: Jiang-Tao
authors.initials: JT
authors.lastname: Chen
authors.affiliation: Department of General Surgery, Huaihe Hospital of HeNan University Kaifeng 475000, Henan Province, China.
authors.forename: Kun-Hou
authors.initials: KH
authors.lastname: Yao
authors.affiliation: Department of General Surgery, Huaihe Hospital of HeNan University Kaifeng 475000, Henan Province, China.
authors.forename: Long
authors.initials: L
authors.lastname: Hua
authors.affiliation: Operating Room, Huaihe Hospital of HeNan University Kaifeng 475000, Henan Province, China.
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Zhang
authors.affiliation: Department of General Surgery, Huaihe Hospital of HeNan University Kaifeng 475000, Henan Province, China.
authors.forename: Chen-Yu
authors.initials: CY
authors.lastname: Wang
authors.affiliation: Department of General Surgery, Huaihe Hospital of HeNan University Kaifeng 475000, Henan Province, China.
authors.forename: Jun-Jie
authors.initials: JJ
authors.lastname: Zhang
mesh_terms: D051722:ADAM Proteins / Q000096:biosynthesis* / Q000235:genetics; D000072198:ADAM17 Protein; D015153:Blotting, Western; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D009361:Neoplasm Invasiveness; D034741:RNA, Small Interfering; D060888:Real-Time Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D014162:Transfection
chemical_list: C532050:MIRN338 microRNA, human; D035683:MicroRNAs; D034741:RNA, Small Interfering; D051722:ADAM Proteins; D000072198:ADAM17 Protein; C000606462:ADAM17 protein, human
keywords: ADAM17; Gastric cancer; miR-338-3p