title: Effect of extraction method on structure and antioxidant activity of Hohenbuehelia serotina polysaccharides.
abstract: The impacts of four extraction methods (hot water, enzyme assistance, ultrasonic assistance and ultrasonic-enzyme assistance) on the extraction yields, preliminary structure and antioxidant activities of the Hohenbuehelia serotina polysaccharides (HW-HSP, EA-HSP, UA-HSP and UEA-HSP) were systematically investigated. The yield of the polysaccharides (20.70±0.17%) obtained by ultrasonic-enzyme assistance was higher than the polysaccharides by other methods'. Four kinds of polysaccharides possessed the different preliminary structural characteristics including molecular weight distributions, monosaccharide compositions, crystallization and spiral structures, while different surface morphology. Through the measurements of antioxidant activities in vitro, UEA-HSP exhibited the most significant scavenging capacities on non-physiological ABTS free radicals and physiological hydroxyl radicals. These data showed that ultrasonic-enzyme assistance was more beneficial to enhance the extraction yields of the polysaccharides, and obtain higher bioactive polysaccharides. The results also suggested that H. serotina polysaccharides possessed potential healthcare application in food field due to their antioxidant activities.
authors.affiliation: School of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao 066004, PR China; School of Food Science and Engineering, Harbin Institute of Technology, Harbin 150090, PR China. Electronic address: lixiaoyu@ysu.edu.cn.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Li
authors.affiliation: School of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao 066004, PR China; School of Food Science and Engineering, Harbin Institute of Technology, Harbin 150090, PR China.
authors.forename: Lu
authors.initials: L
authors.lastname: Wang
mesh_terms: D000363:Agaricales / Q000737:chemistry*; D052160:Benzothiazoles / Q000737:chemistry; D002480:Cellulase / Q000378:metabolism; D005591:Chemical Fractionation / Q000379:methods*; D016166:Free Radical Scavengers / Q000737:chemistry* / Q000302:isolation & purification*; D006358:Hot Temperature; D057446:Hydrodynamics; D017665:Hydroxyl Radical / Q000737:chemistry; D010084:Oxidation-Reduction; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification*; D013451:Sulfonic Acids / Q000737:chemistry; D000069453:Ultrasonic Waves; D014867:Water / Q000737:chemistry*
chemical_list: D052160:Benzothiazoles; D016166:Free Radical Scavengers; D011134:Polysaccharides; D013451:Sulfonic Acids; D014867:Water; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D017665:Hydroxyl Radical; D002480:Cellulase
keywords: Antioxidant activity; Extraction method; Hohenbuehelia serotina; Polysaccharides; Preliminary structure