title: Determination of Vitamin D Serum Levels and Status of the C3435T Polymorphism of Multidrug Resistance 1 Gene in Southeastern Iranian Patients with Ulcerative Colitis.
abstract: BACKGROUND Ulcerative colitis (UC) is a multi-factorial autoimmune disease. P-glycoprotein is encoded by the multidrug resistance 1 (MDR1) gene. The C3435T polymorphism in the MDR1 gene is correlated with low P-glycoprotein expression. Additionally, vitamin D has regulatory effects on the immune system. The aim of our study was to determine the association between the C3435T MDR1 polymorphism and UC and to detect the vitamin D serum levels in patients with UC. METHODS One hundred healthy controls and 85 patients with UC were evaluated. Polymerase chain reaction-restriction fragment length polymorphism (PCRRFLP) was used to detect the C3435T MDR1 polymorphisms. Serum levels of vitamin D were measured by Enzyme-linked immunosorbent assay (ELISA). The research was performed in Kerman, Iran, from 2011 to 2013. RESULTS We could not find any association between the C3435T MDR1 polymorphism and susceptibility to UC. There was a significant decrease in serum levels of vitamin D in patients with UC compared with healthy controls (p<0.001). CONCLUSION Controversies regarding the association between the C3435T MDR1 polymorphism with UC have been reported in different populations. The difference between our results and others may be attributed to the heterogeneity of the Iranian population and the sample size. Additionally, our data indicated that UC might be correlated with vitamin D insufficiency. Therefore, the administration of vitamin D might be suggested as a valuable treatment for patients with UC.
authors.affiliation: Allergy Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran ; Physiology Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran.
authors.forename: Mojgan
authors.initials: M
authors.lastname: Mohammadi
authors.affiliation: Department of Gastroenterology, Afzalipour Hospital, Kerman University of Medical Sciences, Kerman, Iran.
authors.forename: Mohammad Javad
authors.initials: MJ
authors.lastname: Zahedi
authors.affiliation: Department of Immunology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Amin Reza
authors.initials: AR
authors.lastname: Nikpoor
authors.affiliation: Pharmaceutics Department, Kerman University of Medical Sciences, Kerman, Iran.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Nazem
authors.affiliation: Pharmaceutics Department, Kerman University of Medical Sciences, Kerman, Iran.
authors.forename: Payam
authors.initials: P
authors.lastname: Khazaeli
authors.affiliation: Department of Gastroenterology, Afzalipour Hospital, Kerman University of Medical Sciences, Kerman, Iran ; Gastroenterology and Hepatology Research Center, Institute of Basic and Clinical Physiology sciences, kerman University of medical Sciences, Kerman, Iran.
authors.forename: Mohammad Mahdi
authors.initials: MM
authors.lastname: Hayatbakhsh
keywords: MDR1 gene polymorphism; Ulcerative colitis; Vitamin D