title: In Vivo Gene-Silencing in Fibrotic Liver by siRNA-Loaded Cationic Nanohydrogel Particles.
abstract: Cationic nanohydrogel particles loaded with anti-Col1α1 siRNA suppress collagen synthesis and deposition in fibrotic mice: Systemically administered 40 nm sized nanogel particles accumulate in collagen-expressing cells in the liver. Their siRNA payload induces a sequence specific in vivo gene knockdown affording an efficient antifibrotic effect in mice with liver fibrosis.
authors.affiliation: Institute of Translational Immunology and Research Center for Immune Therapy (FZI), University Medical Center of the Johannes Gutenberg-University Mainz, Langenbeckstraße 1, 55101, Mainz, Germany.
authors.forename: Leonard
authors.initials: L
authors.lastname: Kaps
authors.affiliation: Institute of Organic Chemistry, Johannes Gutenberg-University Mainz, Duesbergweg 10-14, 55128, Mainz, Germany.|Department of Pharmaceutics, Ghent University, Ottergemsesteenweg 460, 9000, Ghent, Belgium.
authors.forename: Lutz
authors.initials: L
authors.lastname: Nuhn
authors.affiliation: Institute of Translational Immunology and Research Center for Immune Therapy (FZI), University Medical Center of the Johannes Gutenberg-University Mainz, Langenbeckstraße 1, 55101, Mainz, Germany.
authors.forename: Misbah
authors.initials: M
authors.lastname: Aslam
authors.affiliation: Institute of Translational Immunology and Research Center for Immune Therapy (FZI), University Medical Center of the Johannes Gutenberg-University Mainz, Langenbeckstraße 1, 55101, Mainz, Germany.
authors.forename: Alexander
authors.initials: A
authors.lastname: Brose
authors.affiliation: Institute of Translational Immunology and Research Center for Immune Therapy (FZI), University Medical Center of the Johannes Gutenberg-University Mainz, Langenbeckstraße 1, 55101, Mainz, Germany.|Department of Medicine I (Gastroenterology Hepatology, and Nephrology), University Medical Center of Johannes Gutenberg-University Mainz, Langenbeckstraße 1, 55101, Mainz, Germany.
authors.forename: Friedrich
authors.initials: F
authors.lastname: Foerster
authors.affiliation: Institute of Translational Immunology and Research Center for Immune Therapy (FZI), University Medical Center of the Johannes Gutenberg-University Mainz, Langenbeckstraße 1, 55101, Mainz, Germany.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Rosigkeit
authors.affiliation: Max Planck Institute for Polymer Research, Ackermannweg 10, 55128, Mainz, Germany.
authors.forename: Patricia
authors.initials: P
authors.lastname: Renz
authors.affiliation: Institute of Translational Immunology and Research Center for Immune Therapy (FZI), University Medical Center of the Johannes Gutenberg-University Mainz, Langenbeckstraße 1, 55101, Mainz, Germany.
authors.forename: Rosario
authors.initials: R
authors.lastname: Heck
authors.affiliation: Institute of Translational Immunology and Research Center for Immune Therapy (FZI), University Medical Center of the Johannes Gutenberg-University Mainz, Langenbeckstraße 1, 55101, Mainz, Germany.
authors.forename: Yong Ook
authors.initials: YO
authors.lastname: Kim
authors.affiliation: Max Planck Institute for Polymer Research, Ackermannweg 10, 55128, Mainz, Germany.
authors.forename: Ingo
authors.initials: I
authors.lastname: Lieberwirth
authors.affiliation: Institute of Translational Immunology and Research Center for Immune Therapy (FZI), University Medical Center of the Johannes Gutenberg-University Mainz, Langenbeckstraße 1, 55101, Mainz, Germany.|Division of Gastroenterology, Beth Israel Deaconess Medical Center, Harvard Medical School, 330 Brookline Avenue, Boston, MA, 02215, USA.
authors.forename: Detlef
authors.initials: D
authors.lastname: Schuppan
authors.affiliation: Institute of Organic Chemistry, Johannes Gutenberg-University Mainz, Duesbergweg 10-14, 55128, Mainz, Germany.
authors.forename: Rudolf
authors.initials: R
authors.lastname: Zentel
mesh_terms: D000818:Animals; D002412:Cations; D002460:Cell Line; D020868:Gene Silencing; D018014:Gene Transfer Techniques; D020100:Hydrogels / Q000737:chemistry*; D008103:Liver Cirrhosis / Q000378:metabolism*; D051379:Mice; D000080385:Nanogels; D053758:Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D011092:Polyethylene Glycols / Q000737:chemistry*; D011094:Polyethyleneimine / Q000737:chemistry*; D034741:RNA, Small Interfering / Q000378:metabolism*; D014018:Tissue Distribution
chemical_list: D002412:Cations; D020100:Hydrogels; D000080385:Nanogels; D034741:RNA, Small Interfering; C413692:polyethylene glycol polyethyleneimine nanogel; D011092:Polyethylene Glycols; D011094:Polyethyleneimine
keywords: cationic nanohydrogels; in vivo gene knockdown; liver fibrosis; nanogels; siRNA delivery