title: Generation of Induced Pluripotent Stem Cells from Mammalian Endangered Species.
abstract: For some highly endangered species there are too few reproductively capable animals to maintain adequate genetic diversity, and extraordinary measures are necessary to prevent their extinction. Cellular reprogramming is a means to capture the genomes of individual animals as induced pluripotent stem cells (iPSCs), which may eventually facilitate reintroduction of genetic material into breeding populations. Here, we describe a method for generating iPSCs from fibroblasts of mammalian endangered species.
authors.affiliation: Department of Chemical Physiology, Center for Regenerative Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA, 92037, USA. fbninbar@gmail.com.
authors.forename: Inbar Friedrich
authors.initials: IF
authors.lastname: Ben-Nun
authors.affiliation: Department of Chemical Physiology, Center for Regenerative Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA, 92037, USA.
authors.forename: Susanne C
authors.initials: SC
authors.lastname: Montague
authors.affiliation: San Diego Zoo Institute for Conservation Research, Escondido, CA, USA.
authors.forename: Marlys L
authors.initials: ML
authors.lastname: Houck
authors.affiliation: San Diego Zoo Institute for Conservation Research, Escondido, CA, USA.
authors.forename: Oliver
authors.initials: O
authors.lastname: Ryder
authors.affiliation: Department of Chemical Physiology, Center for Regenerative Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA, 92037, USA.|Department of Reproductive Medicine, University of California, San Diego, La Jolla, CA, USA.
authors.forename: Jeanne F
authors.initials: JF
authors.lastname: Loring
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D065150:Cellular Reprogramming; D056727:Endangered Species; D061252:Feeder Cells; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism*; D005822:Genetic Vectors / Q000235:genetics; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism*; D051379:Mice; D012190:Retroviridae / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics; D014161:Transduction, Genetic; D019076:Transgenes
chemical_list: D014157:Transcription Factors
keywords: Cellular reprogramming; Conservation; Endangered species; Induced pluripotent stem cells