title: Elevated Plasma P-Selectin Autoantibodies in Primary Sjögren Syndrome Patients with Thrombocytopenia.
abstract: BACKGROUND Primary Sjögren's syndrome (pSS) is one of the most common chronic systemic autoimmune diseases, and thrombocytopenia is one of the hematological manifestations of pSS. When platelet and endothelial cells are activated, P-selectin is expressed on the cell surface. This study aimed to investigate the role of P-selectin autoantibodies in the pathogenesis of thrombocytopenia in pSS. MATERIAL AND METHODS P-selectin autoantibodies were measured by enzyme-linked immunosorbent assay (ELISA) in 38 pSS patients without thrombocytopenia and 32 pSS patients with thrombocytopenia, 32 idiopathic thrombocytopenic purpura (ITP) patients, and 35 healthy controls. RESULTS The plasma P-selectin autoantibodies (A490) in ITP patients and pSS patients with/without thrombocytopenia were significantly higher than those in healthy controls, but there were no significant differences between ITP patients and pSS patients with thrombocytopenia. The positive rate of P-selectin autoantibodies in pSS patients with thrombocytopenia was significantly higher than that in ITP patients. The platelet count was lower in P-selectin autoantibodies-positive patients, while among pSS patients with thrombocytopenia, the platelet count was lower in P-selectin autoantibodies-positive patients than in P-selectin autoantibodies-negative patients. In ITP patients and pSS patients with thrombocytopenia, the platelet count was lower in P-selectin autoantibodies-positive patients. CONCLUSIONS Elevated plasma P-selectin autoantibodies may play a role in the pathogenesis of thrombocytopenia in pSS patients.
authors.affiliation: School of Medical Laboratory, Tianjin Medical University, Tianjin, China (mainland).
authors.forename: Ya-Hui
authors.initials: YH
authors.lastname: Hu
authors.affiliation: School of Medical Laboratory, Tianjin Medical University, Tianjin, China (mainland).
authors.forename: Peng-Fei
authors.initials: PF
authors.lastname: Zhou
authors.affiliation: School of Medical Laboratory, Tianjin Medical University, Tianjin, China (mainland).
authors.forename: Guang-Feng
authors.initials: GF
authors.lastname: Long
authors.affiliation: School of Medical Laboratory, Tianjin Medical University, Tianjin, China (mainland).
authors.forename: Xin
authors.initials: X
authors.lastname: Tian
authors.affiliation: Department of Rheumatology and Immunology, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China (mainland).
authors.forename: Yu-Fan
authors.initials: YF
authors.lastname: Guo
authors.affiliation: Hematopoietic Stem Cell Transplantation Center, Institute of Hematology and Blood Diseases Hospital, Tianjin, China (mainland).
authors.forename: Ai-Ming
authors.initials: AM
authors.lastname: Pang
authors.affiliation: Hubei University of Chinese Medicine, Wuhan, Hubei, China (mainland).
authors.forename: Ran
authors.initials: R
authors.lastname: Di
authors.affiliation: School of Medical Laboratory, Tianjin Medical University, Tianjin, China (mainland).
authors.forename: Yan-Na
authors.initials: YN
authors.lastname: Shen
authors.affiliation: School of Medical Laboratory, Tianjin Medical University, Tianjin, China (mainland).
authors.forename: Yun-De
authors.initials: YD
authors.lastname: Liu
authors.affiliation: School of Medical Laboratory, Tianjin Medical University, Tianjin, China (mainland).
authors.forename: Yu-Jie
authors.initials: YJ
authors.lastname: Cui
mesh_terms: D000293:Adolescent; D000328:Adult; D001323:Autoantibodies / Q000097:blood* / Q000276:immunology; D001792:Blood Platelets / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D008875:Middle Aged; D019007:P-Selectin / Q000276:immunology*; D010976:Platelet Count; D012859:Sjogren's Syndrome / Q000097:blood* / Q000276:immunology; D013921:Thrombocytopenia / Q000097:blood* / Q000276:immunology
chemical_list: D001323:Autoantibodies; D019007:P-Selectin; C546937:SELP protein, human