title: [The Development and Application of BODIPY-Labeled Oligonucleotides in Real-Time Polymerase Chain Reaction].
abstract: A BODIPY-based (4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene, TMB) green fluorescent probe for quantitative real-time polymerase chain reaction (qPCR) was synthesized by azide-alkyne cycloaddition reaction. Comparative studies with analogous fluorescein-based probe were carryed out. We demonstrate that fluorescent probes with TMB fluorophore can be used in qPCR experiments as well.
authors.forename: E V
authors.initials: EV
authors.lastname: Ilnitskaya
authors.forename: Yu N
authors.initials: YN
authors.lastname: Kononevich
authors.forename: A M
authors.initials: AM
authors.lastname: Muzafarov
authors.forename: S A
authors.initials: SA
authors.lastname: Rzhevskiy
authors.forename: I A
authors.initials: IA
authors.lastname: Shadrin
authors.forename: E V
authors.initials: EV
authors.lastname: Babaev
authors.forename: V I
authors.initials: VI
authors.lastname: Martynov
authors.forename: A A
authors.initials: AA
authors.lastname: Pakhomov
mesh_terms: D001896:Boron Compounds / Q000737:chemistry*; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000737:chemistry; D060888:Real-Time Polymerase Chain Reaction
chemical_list: C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D009841:Oligonucleotides