title: Application of the BIOLOG system for characterization of Serratia marcescens ss marcescens isolated from onsite wastewater technology (OSWT).
abstract: The scope of this study was to apply the Biolog system to identify and characterize a Serratia strain isolated from the surface of black plastic pieces which constitute the fluidized bed filter (onsite wastewater technology, OSWT). The preliminary isolation of the strain was done in the medium with tetracycline at a 16 mg/l concentration. To characterize the isolated strain, the following Biolog methods were applied: (1) EcoPlates microplates for evaluation of physiological profiling, (2) GEN III OmniLog® ID System for identification of the isolate, and (3) phenotypic microarrays (PM) technology for evaluation of sensitivity to antibiotics (PM11 and PM12). Results were recorded using the original OmniLog® software. The Serratia strain was identified as Serratia marcescens ss marcescens with similarity index 0.569. The same identification was obtained by the 16S rDNA analysis. PM analysis showed an enhancement of phenotype (resistance or growth) of this strain to 35 antibiotics. The loss of phenotype (sensitivity or non-growth) was observed only for 5 antibiotics: lomefloxacin (0.4 µg/ml), enoxacin (0.9 µg/ml), nalidixic acid (18.0 µg/ml), paromomycin (25.0 µg/ml) and novobiocin (1100 µg/ml). This study acknowledges that the methods proposed by the Biolog system allow correct and complete identification and characterization of the microbes isolated from different environments. Phenotypic microarrays could be successfully used as a new tool for identification of the multi-antibiotic resistance of bacteria and for determination of the minimal inhibition concentrations (MIC).
authors.affiliation: Department of Environmental Microbiology, Institute for Ecology of Industrial Areas, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Chojniak
authors.affiliation: Department of Environmental Microbiology, Institute for Ecology of Industrial Areas, Poland.
authors.forename: Łukasz
authors.initials: Ł
authors.lastname: Jałowiecki
authors.affiliation: Development and Assessment Institute in Waste Water Technology at RWTH Aachen University, Germany.
authors.forename: Elmar
authors.initials: E
authors.lastname: Dorgeloh
authors.affiliation: Development and Assessment Institute in Waste Water Technology at RWTH Aachen University, Germany.
authors.forename: Berta
authors.initials: B
authors.lastname: Hegedusova
authors.affiliation: Natural Resources & Environmental Effects, IVL Swedish Environmental Research Institute, Sweden.
authors.forename: Helene
authors.initials: H
authors.lastname: Ejhed
authors.affiliation: Natural Resources & Environmental Effects, IVL Swedish Environmental Research Institute, Sweden.
authors.forename: Jörgen
authors.initials: J
authors.lastname: Magnér
authors.affiliation: Department of Environmental Microbiology, Institute for Ecology of Industrial Areas, Poland.
authors.forename: Grażyna
authors.initials: G
authors.lastname: Płaza
mesh_terms: D003470:Culture Media; D004352:Drug Resistance, Microbial; D012706:Serratia marcescens / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification*; D062065:Wastewater / Q000382:microbiology*
chemical_list: D003470:Culture Media; D062065:Wastewater