title: DDX5/p68 RNA helicase expression is essential for initiating adipogenesis.
abstract: BACKGROUND
  DDX5/p68 RNA helicase is a member of the DEAD (Asp-Glu-Ala-Asp) box proteins. Apart from RNA unwinding, DDX5 is an important transcriptional factor and co-activator in cell proliferation and differentiation.
  
  
  RESULTS
  Here, we have characterised the role of DDX5 in adipogenesis in 3T3-L1 cells using siRNA mediated suppression. Transient inhibition of Ddx5 mRNA expression at the start of adipogenesis impairs the differentiation programme even when DDX5 expression is restored later in adipogenesis. However transient suppression of Ddx5 at the later stages of adipogenesis do not impair adipogenesis or triglyceride accumulation suggesting Ddx5 expression is dispensable in a mature adipocyte.
  
  
  CONCLUSIONS
  These results implicate DDX5 as a crucial factor involved in the complex transcriptional cascade of events that regulate adipogenesis and essential to the initiation of adipogenesis.
authors.affiliation: Developmental and Regenerative Biology Laboratory, Institute of Medical Biology, #06-06, 8A Biomedical Grove, Singapore, 138648, Republic of Singapore. nardevr@gmail.com.|Present address: Davos Life Science Pte Ltd, 3 Biopolis Drive, #04-19, Synapse, Singapore, 138623, Republic of Singapore. nardevr@gmail.com.
authors.forename: Nardev
authors.initials: N
authors.lastname: Ramanathan
authors.affiliation: Developmental and Regenerative Biology Laboratory, Institute of Medical Biology, #06-06, 8A Biomedical Grove, Singapore, 138648, Republic of Singapore. nicolelsx@gmail.com.|School of Biological Sciences, College of Science, Nanyang Technological University, Singapore, 637551, Republic of Singapore. nicolelsx@gmail.com.
authors.forename: Nicole
authors.initials: N
authors.lastname: Lim
authors.affiliation: Developmental and Regenerative Biology Laboratory, Institute of Medical Biology, #06-06, 8A Biomedical Grove, Singapore, 138648, Republic of Singapore. colin.stewart@imb.a-star.edu.sg.|School of Biological Sciences, College of Science, Nanyang Technological University, Singapore, 637551, Republic of Singapore. colin.stewart@imb.a-star.edu.sg.
authors.forename: Colin L
authors.initials: CL
authors.lastname: Stewart
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000201:enzymology; D050156:Adipogenesis; D000818:Animals; D053487:DEAD-box RNA Helicases / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D055785:Gene Knockdown Techniques; D059630:Mesenchymal Stem Cells / Q000201:enzymology* / Q000502:physiology; D051379:Mice; D008809:Mice, Inbred C3H
chemical_list: C506751:Ddx5 protein, mouse; D053487:DEAD-box RNA Helicases