title: Intrinsic kinetic parameters of Thermococcus onnurineus NA1 strains and prediction of optimum carbon monoxide level for ideal bioreactor operation.
abstract: This study determines and compares the intrinsic kinetic parameters (Ks and Ki) of selected Thermococcus onnurineus NA1 strains (wild-type (WT), and mutants MC01, MC02, and WTC156T) using the substrate inhibition model. Ks and Ki values were used to find the optimum dissolved CO (CL) conditions inside the reactor. The results showed that in terms of the maximum specific CO consumption rates (qCO(max)) of WT, MC01, MC02, and WTC156T the optimum activities can be achieved by maintaining the CL levels at 0.56mM, 0.52mM, 0.58mM, and 0.75mM, respectively. The qCO(max) value of WTC156T at 0.75mM was found to be 1.5-fold higher than for the WT strain, confirming its superiority. Kinetic modeling was then used to predict the conditions required to maintain the optimum CL levels and high cell concentrations in the reactor, based on the kinetic parameters of the WTC156T strain.
authors.affiliation: School of Environmental Science and Engineering, Gwangju Institute of Science and Technology (GIST), Gwangju 61005, Republic of Korea.
authors.forename: Yeseul
authors.initials: Y
authors.lastname: Jeong
authors.affiliation: School of Environmental Science and Engineering, Gwangju Institute of Science and Technology (GIST), Gwangju 61005, Republic of Korea.
authors.forename: Nulee
authors.initials: N
authors.lastname: Jang
authors.affiliation: School of Environmental Science and Engineering, Gwangju Institute of Science and Technology (GIST), Gwangju 61005, Republic of Korea; Department of Chemical Engineering, COMSATS Institute of Information Technology (CIIT), Lahore, Pakistan.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Yasin
authors.affiliation: School of Environmental Science and Engineering, Gwangju Institute of Science and Technology (GIST), Gwangju 61005, Republic of Korea.
authors.forename: Shinyoung
authors.initials: S
authors.lastname: Park
authors.affiliation: School of Environmental Science and Engineering, Gwangju Institute of Science and Technology (GIST), Gwangju 61005, Republic of Korea. Electronic address: ischang@gist.ac.kr.
authors.forename: In Seop
authors.initials: IS
authors.lastname: Chang
mesh_terms: D019149:Bioreactors; D002248:Carbon Monoxide / Q000032:analysis / Q000378:metabolism*; D006859:Hydrogen / Q000378:metabolism*; D007700:Kinetics; D009154:Mutation; D019712:Thermococcus / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*
chemical_list: D002248:Carbon Monoxide; D006859:Hydrogen
keywords: Biohydrogen; Bioreactor operation; Carbon monoxide inhibition; Kinetic modeling; Thermococcus onnurineus NA1