title: Apoptotic effects of Photofrin-Diomed 630-PDT on SHEEC human esophageal squamous cancer cells.
abstract: Photodynamic therapy (PDT) using photofrin-II is a clinically effective treatment for both non-neoplastic and neoplastic diseases. Herein, we performed an in vitro experiment to study the anti-tumor effect and mechanisms of photofrin-II mediated PDT for esophageal squamous cell carcinoma (ESCC) cell line, SHEEC. In this study, human ESCC cell line SHEEC and parental normal cell line SHEE were used. The anti-tumor effect of PDT was determined by evaluating cell viability using CCK-8 assay, apoptosis and generation of reactive oxygen species (ROS). PDT induced significant apoptosis in SHEEC and SHEE cells in a time- and photofrin-II dose-dependent manner. Furthermore, PDT treatment induced significant death of SHEEC, instead of SHEE cells. The apoptotic outcome was accompanied by concurrent generation of ROS. In summary, PDT shed light on therapy of ESCC, functioning as a useful tool for ESCC clinical treatment, providing a better understanding of Photofrin-Diomed 630-PDT in SHEEC cells.
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Henan University of Science and Technology No. 24 Jinghua Road, Luoyang, Henan, China.
authors.forename: Shegan
authors.initials: S
authors.lastname: Gao
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Henan University of Science and Technology No. 24 Jinghua Road, Luoyang, Henan, China.
authors.forename: Mengxi
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Henan University of Science and Technology No. 24 Jinghua Road, Luoyang, Henan, China.
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Zhu
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Henan University of Science and Technology No. 24 Jinghua Road, Luoyang, Henan, China.
authors.forename: Zhifeng
authors.initials: Z
authors.lastname: Qu
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Henan University of Science and Technology No. 24 Jinghua Road, Luoyang, Henan, China.
authors.forename: Tanyou
authors.initials: T
authors.lastname: Shan
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Henan University of Science and Technology No. 24 Jinghua Road, Luoyang, Henan, China.
authors.forename: Xuanhu
authors.initials: X
authors.lastname: Xie
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Henan University of Science and Technology No. 24 Jinghua Road, Luoyang, Henan, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Henan University of Science and Technology No. 24 Jinghua Road, Luoyang, Henan, China.
authors.forename: Xiaoshan
authors.initials: X
authors.lastname: Feng
keywords: PDT; ROS; SHEE; SHEEC; apoptosis; photofrin-II