title: Effect of proximal versus distal 50% enterectomy on nutritional parameters in rats preconditioned with a high-fat diet or regular chow.
abstract: Obesity may protect against the nutritional consequences of short bowel syndrome. We hypothesized that rats preconditioned with an obesogenic diet would have better outcomes after surgical induction of short bowel syndrome compared to rats on regular chow. Rats were fed a high-fat diet or regular rat chow for six months, and then underwent 50% proximal, 50% distal, or sham enterectomy. Food intake, weight, and body composition were monitored before and for 4 weeks after surgery. The high-fat diet consistently produced obesity (>25% body fat). All procedures induced weight loss, but there was no discernable difference between resection vs. sham resection. Rats on the high-fat diet had a greater post-resection loss of body fat compared to rats on chow (36 vs. 26 g, respectively). There was a nonsignificant trend of less lean mass loss in the former compared to the latter rats (16 vs. 33 g, respectively). Enterectomy moderated serum ghrelin, GIP, PPY, insulin, and leptin. Intestinal adaptation was not different between obese vs. non-obese rats. Rats preconditioned with the high-fat diet may have had better retention of lean body mass after a surgical procedure compared to rats on chow. The effect of 50% enterectomy was less than expected.
authors.affiliation: Department of Surgery, University of Nebraska Medical Center, Omaha, NE 68198.|Department of Surgery, VA-Nebraska Western Iowa Health Care System, Omaha, NE 68105.
authors.forename: Ujwal R
authors.initials: UR
authors.lastname: Yanala
authors.affiliation: Department of Surgery, VA-Nebraska Western Iowa Health Care System, Omaha, NE 68105.|Department of Biomedical Sciences, Creighton University, Omaha, NE 68178.
authors.forename: Roger D
authors.initials: RD
authors.lastname: Reidelberger
authors.affiliation: Department of Surgery, University of Nebraska Medical Center, Omaha, NE 68198.
authors.forename: Jon S
authors.initials: JS
authors.lastname: Thompson
authors.affiliation: Department of Biostatistics, University of Nebraska Medical Center, Omaha, NE 68198.
authors.forename: Valerie K
authors.initials: VK
authors.lastname: Shostrom
authors.affiliation: Department of Surgery, University of Nebraska Medical Center, Omaha, NE 68198.|Department of Surgery, VA-Nebraska Western Iowa Health Care System, Omaha, NE 68105.|Department of Genetics, Cell Biology, and Anatomy, University of Nebraska Medical Center, Omaha, NE 68198.
authors.forename: Mark A
authors.initials: MA
authors.lastname: Carlson
mesh_terms: D000818:Animals; D050218:Body Fat Distribution; D059305:Diet, High-Fat / Q000009:adverse effects*; D004435:Eating; D002149:Energy Intake; D005749:Gastric Inhibitory Polypeptide / Q000097:blood; D054439:Ghrelin / Q000097:blood; D007328:Insulin / Q000097:blood; D007421:Intestine, Small / Q000601:surgery*; D020738:Leptin / Q000097:blood; D008297:Male; D009765:Obesity / Q000097:blood* / Q000209:etiology / Q000473:pathology; D010191:Pancreatic Polypeptide / Q000097:blood; D051381:Rats; D017207:Rats, Sprague-Dawley; D012778:Short Bowel Syndrome / Q000097:blood* / Q000473:pathology / Q000601:surgery
chemical_list: D054439:Ghrelin; D007328:Insulin; D020738:Leptin; D005749:Gastric Inhibitory Polypeptide; D010191:Pancreatic Polypeptide