title: Correlation between blood and lymphatic vessel density and results of contrast-enhanced spectral mammography.
abstract: Contrast-enhanced spectral mammography (CESM) is a novel technique used for detection of tumour vascularity by imaging the moment in which contrast, delivered to the lesion by blood vessels, leaks out of them, and flows out through lymphatic vessels. In our study, we included 174 women for whom spectral mammography was performed for diagnostic purposes. The relationship between enhancement in CESM and blood vessel density (BVD), lymphatic vessel density (LVD) or the percentage of fields with at least one lymphatic vessel (distribution of podoplanin-positive vessels - DPV) and other related parameters was assessed in 55 cases. BVD, LVD and DPV were assessed immunohistochemically, applying podoplanin and CD31/CD34 as markers of lymphatic and blood vessels, respectively. The sensitivity (in detection of malignant lesions) of CESM was 100%, while its specificity - 39%. We found a significant positive correlation between the intensity of enhancement in CESM and BVD (p = 0.007, r = 0.357) and a negative correlation between the intensity of enhancement in CESM and DPV (p = 0.003, r = -0.390). Lesions with the highest enhancement in CESM showed a high number of blood vessels and a low number of lymphatics.
  
  
  CONCLUSIONS
  1) CESM is a method characterized by high sensitivity and acceptable specificity; 2) the correlation between CESM results and blood/lymphatic vessel density confirms its utility in detection of tissue angiogenesis and/or lymphangiogenesis.
authors.affiliation: Elżbieta Łuczyńska MD, PhD, Radiology Department Center of Oncology, M. Sklodowska-Curie Memorial Institute, Kracow Branch, Garncarska 11, 31-115 Krakow, Poland, tel. +48 12 422 99 00, fax +48 12 421 23 25, e-mail: z5luczyn@cyfronet.pl.
authors.forename: Elzbieta
authors.initials: E
authors.lastname: Luczynska
authors.forename: Joanna
authors.initials: J
authors.lastname: Niemiec
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Ambicka
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Adamczyk
authors.forename: Tomasz
authors.initials: T
authors.lastname: Walasek
authors.forename: Janusz
authors.initials: J
authors.lastname: Ryś
authors.forename: Beata
authors.initials: B
authors.lastname: Sas-Korczyńska
mesh_terms: D001940:Breast / Q000098:blood supply; D001943:Breast Neoplasms / Q000098:blood supply / Q000000981:diagnostic imaging* / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D042601:Lymphatic Vessels / Q000000981:diagnostic imaging*; D008327:Mammography / Q000379:methods*; D008875:Middle Aged