title: MicroRNA-214 induces dendritic cell switching from tolerance to immunity by targeting β-Catenin signaling.
abstract: MicroRNAs (miRNAs) are known to function as negative gene regulators. Recently, miRNAs have been shown to regulate immunity processes; however, the mechanism is unclear. The role of microRNA-214 (miR-214) in dendritic cell (DC) maturation has not been investigated. We found that the miR-214 level was correlated with the maturation of DCs and inflammatory cytokine secretion, as depressed miR-214 levels induced DC tolerance. We also identified β-catenin as a target gene of miR-214 and demonstrated its association with Treg cell differentiation. MiR-214 regulates gene expression by binding to the 3'UTR of β-catenin. The results suggest that β-catenin is a critical regulator of tolerance in DCs via miR-214. The expression of miR-214 could be a potential therapeutic strategy in organ transplantation or autoimmunity patients.
authors.affiliation: Department of Medical Biology, College of Basic Medicine, Tianjin Medical University Tianjin 300070, People's Republic of China.
authors.forename: Chao
authors.initials: C
authors.lastname: Gu
authors.affiliation: Laboratory of Flow Cytometry, Tianjin Institute of Urology Tianjin 300211, People's Republic of China.
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Zhou
authors.affiliation: Department of Physical Diagnosis, Tianjin Hospital Tianjin 300211, People's Republic of China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: Department of Medical Biology, College of Basic Medicine, Tianjin Medical University Tianjin 300070, People's Republic of China.
authors.forename: Xu-Hong
authors.initials: XH
authors.lastname: Miao
authors.affiliation: Department of Medical Biology, College of Basic Medicine, Tianjin Medical University Tianjin 300070, People's Republic of China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Medical Biology, College of Basic Medicine, Tianjin Medical University Tianjin 300070, People's Republic of China.
authors.forename: Ya-Wei
authors.initials: YW
authors.lastname: Ru
authors.affiliation: Department of Medical Biology, College of Basic Medicine, Tianjin Medical University Tianjin 300070, People's Republic of China.
authors.forename: Ke-Qiu
authors.initials: KQ
authors.lastname: Li
authors.affiliation: Department of Medical Biology, College of Basic Medicine, Tianjin Medical University Tianjin 300070, People's Republic of China.
authors.forename: Guang
authors.initials: G
authors.lastname: Li
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism*; D007108:Immune Tolerance / Q000235:genetics*; D051379:Mice; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000235:genetics*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology / Q000378:metabolism; D060449:Wnt Signaling Pathway / Q000235:genetics; D051176:beta Catenin / Q000378:metabolism*
chemical_list: D035683:MicroRNAs; C534781:Mirn214 microRNA, mouse; D051176:beta Catenin
keywords: MicroRNA-214; Treg cells; dendritic cells; transplantation tolerance; β-Catenin