title: A two pathway model for N2O emissions by ammonium oxidizing bacteria supported by the NO/N2O variation.
abstract: In this work, a new model for nitritation combining two N2O emission pathways was confronted with both NO and N2O measurements during nitrification. The model was calibrated with batch experiments and validated with long-term data collected in a sequencing batch reactor (SBR). A good prediction of the evolution of N2O emissions for a varying level of nitrite was demonstrated. The NO/N2O ratio was shown to vary during nitritation depending on the nitrite level. None of the models based on a single pathway could describe this variation of the NO/N2O ratio. In contrast, the 2 pathway model was capable of describing the trends observed for the NO/N2O ratio and gave better predictions of N2O emission factors. The model confirmed that the decrease of the NO/N2O ratio can be explained by an increase of the ND pathway to the detriment of the NN pathway. The ND pathway was systematically the predominant pathway during nitritation. The combined effect of nitrite (or free nitrous acid) and dissolved oxygen (DO) on the contribution of each pathway was in agreement with practical observations and the literature.
authors.affiliation: Université de Toulouse; INSA, UPS, LISBP, 135 Avenue de Rangueil, F-31077, Toulouse, France; INRA, UMR792, Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés, F-31400 Toulouse, France; CNRS, UMR5504, F-31400, Toulouse, France; CNRS; LAAS; 7 avenue du colonel Roche, F-31077, Toulouse Cedex 4, France.
authors.forename: M
authors.initials: M
authors.lastname: Pocquet
authors.affiliation: Université de Toulouse; INSA, UPS, LISBP, 135 Avenue de Rangueil, F-31077, Toulouse, France; INRA, UMR792, Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés, F-31400 Toulouse, France; CNRS, UMR5504, F-31400, Toulouse, France.
authors.forename: Z
authors.initials: Z
authors.lastname: Wu
authors.affiliation: CNRS; LAAS; 7 avenue du colonel Roche, F-31077, Toulouse Cedex 4, France.
authors.forename: I
authors.initials: I
authors.lastname: Queinnec
authors.affiliation: Université de Toulouse; INSA, UPS, LISBP, 135 Avenue de Rangueil, F-31077, Toulouse, France; INRA, UMR792, Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés, F-31400 Toulouse, France; CNRS, UMR5504, F-31400, Toulouse, France. Electronic address: sperandio@insa-toulouse.fr.
authors.forename: M
authors.initials: M
authors.lastname: Spérandio
mesh_terms: D000332:Aerobiosis; D064751:Ammonium Compounds / Q000378:metabolism*; D001419:Bacteria / Q000378:metabolism*; D019149:Bioreactors; D008962:Models, Theoretical; D009569:Nitric Oxide / Q000378:metabolism*; D058465:Nitrification; D009573:Nitrites / Q000032:analysis; D009609:Nitrous Oxide / Q000032:analysis / Q000378:metabolism*; D010084:Oxidation-Reduction; D010100:Oxygen / Q000032:analysis
chemical_list: D064751:Ammonium Compounds; D009573:Nitrites; D009569:Nitric Oxide; D009609:Nitrous Oxide; D010100:Oxygen
keywords: Ammonia oxidizing bacteria; Modelling; Nitric oxide; Nitritation; Nitrous oxide; Production pathways