title: Production, purification and characterization of novel beta glucosidase from newly isolated Penicillium simplicissimum H-11 in submerged fermentation.
abstract: β-Glucosidase is an important component of the cellulase complex. It not only hydrolyzes cellobiose and short-chain cellooligosaccharides to glucose, but also removes the inhibitory effect of cellobiose on the β-1, 4-endoglucanase and exoglucanase, thereby increasing the overall rate of cellulose biodegradation. β-glucosidasefrom culture supernatant of a fungus Penicillium simplicissimum was purified to homogeneity, by using ammonium sulfate fraction, Sephadex G-100 chromatography, and its properties were studied. The molecular mass of the enzyme was about 126.0 kDa, as identified by 12% SDS-PAGE. The optimum pH and temperature were 4.4 ~ 5.2 and 60 °C, respectively. The enzyme was stable in pH 5.2 ~ 6.4 and under 40 °C. Metal profile of the enzyme showed that Mn(2+) enhances its activity, while Cu(2+), Co(2+)and Fe(3+) cause obvious inhibition. The K m and V max was 14.881 mg/ml and 0.364 mg ml/min against salicin as a Substrate. This enzyme had secondary protein structure as evidenced by FTIR spectrum.
authors.affiliation: School of Land and environment, Shenyang Agricultural University, Shenyang 110866 Liaoning, P.R. China.
authors.forename: Hongzhi
authors.initials: H
authors.lastname: Bai
authors.affiliation: Bioscience and Biotechnology College, Shenyang Agricultural University, Shenyang 110866 Liaoning, P.R. China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.affiliation: School of Land and environment, Shenyang Agricultural University, Shenyang 110866 Liaoning, P.R. China.
authors.forename: Junde
authors.initials: J
authors.lastname: Sun
authors.affiliation: Bioscience and Biotechnology College, Shenyang Agricultural University, Shenyang 110866 Liaoning, P.R. China.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Irfan
authors.affiliation: School of Land and environment, Shenyang Agricultural University, Shenyang 110866 Liaoning, P.R. China.
authors.forename: Mei
authors.initials: M
authors.lastname: Han
authors.affiliation: School of Land and environment, Shenyang Agricultural University, Shenyang 110866 Liaoning, P.R. China.
authors.forename: Yuqian
authors.initials: Y
authors.lastname: Huang
authors.affiliation: School of Land and environment, Shenyang Agricultural University, Shenyang 110866 Liaoning, P.R. China.
authors.forename: Xiaori
authors.initials: X
authors.lastname: Han
authors.affiliation: Center of Life Science and Technology Harbin Institute of Technology Harbin, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Yang
keywords: Penicillium simplicissimum; beta-glucosidase; characterization; purification