title: A Novel Cell Therapy Method for Recovering after Brain Stroke in Rats.
abstract: BACKGROUND
  Nowadays, stroke leads to a significant part of the adult mortality and morbidity and also it could result in some neurological deficits in the patients' lives. Cell therapy has opened a new approach to treat the brain ischemia and reduce its terrible effects on the patients' lives. There are several articles which show that the cell therapy could be beneficial for treating brain stroke. In this study, we have planned to present a new cell therapy method for stroke by administration of Mesenchymal stem cells and differentiated neural stem cells without astrocytes.
  
  
  METHODS
  The Mesenchymal stem cells were isolated from tibia and femur of a 250~300 g rat and they were cultured in DMEM/F12, 10% fetal bovine serum, 1% Pen/Strep. Neural stem cells were isolated from 14 days rat embryo ganglion eminence and were cultured in NSA media containing Neurobasal, 2% B27, bFGF 10 ng/ml and EGF 20 ng/ml after 5 days they formed some neurospheres. The isolated neural stem cells were differentiated to neural lineages by adding 5% fetal bovine serum to their culture media. After 48 hours the astrocytes were depleted by using MACS kit.
  
  
  RESULTS
  The group that received Mesenchymal stem cells systemically and differentiated neural stem cells without astrocytes had the best neurological outcomes and the least infarct volume and apoptosis. It could be understood that this cell therapy method might cause almost full recovery after brain stoke.
  
  
  CONCLUSIONS
  Using combination cell therapy with Mesenchymal stem cells and differentiated neural stem cells with removed astrocyte could provide a novel method for curing brain stroke.
authors.affiliation: Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran ; Cell & Molecular Medicine Student Research Group, Medical School, Shiraz University of Medical Sciences, Shiraz, Iran ; Stem Cell Laboratory, Department of Anatomy, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Seyed Mojtaba
authors.initials: SM
authors.lastname: Hosseini
authors.affiliation: Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran ; Cell & Molecular Medicine Student Research Group, Medical School, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Farahmandnia
authors.affiliation: Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran ; Cell & Molecular Medicine Student Research Group, Medical School, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Sepehr
authors.initials: S
authors.lastname: Kazemi
authors.affiliation: Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran ; Cell & Molecular Medicine Student Research Group, Medical School, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Benafshe
authors.initials: B
authors.lastname: Shakibajahromi
authors.affiliation: Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran ; Cell & Molecular Medicine Student Research Group, Medical School, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Fatemeh Sabet
authors.initials: FS
authors.lastname: Sarvestani
authors.affiliation: Trangenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Khodabande
keywords: Astrocyte; Brain stroke; Mesenchymal stem cells; Neural stem cells