title: Recovery of Replication-Competent and G-Gene-Deleted Rabies Viruses from cDNA.
abstract: This protocol describes the recovery of replication-competent rabies viruses (RV) such as SAD L16 or SAD L16-eGFP. It is suggested that at least three parallel transfection experiments are performed to increase the success rate (in three 3.5-cm(2) dishes or in three wells in a six-well plate). The entire protocol takes 10 d, and successful rescue can be obtained after 6 d. An additional procedure for the recovery of G-gene-deleted viruses is also included. Please note that appropriate biosafety measures are needed.
authors.forename: Nadin
authors.initials: N
authors.lastname: Hagendorf
authors.forename: Karl-Klaus
authors.initials: KK
authors.lastname: Conzelmann
mesh_terms: D000956:Antigens, Viral / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D017353:Gene Deletion; D006023:Glycoproteins / Q000172:deficiency* / Q000235:genetics; D011820:Rabies virus / Q000235:genetics* / Q000502:physiology*; D059386:Reverse Genetics / Q000379:methods*; D014759:Viral Envelope Proteins / Q000172:deficiency* / Q000235:genetics; D014779:Virus Replication
chemical_list: D000956:Antigens, Viral; D018076:DNA, Complementary; D004279:DNA, Viral; D006023:Glycoproteins; D014759:Viral Envelope Proteins; C064951:glycoprotein G, Rabies virus