title: Isolation and Comparative Genomic Analysis of T1-Like Shigella Bacteriophage pSf-2.
abstract: The increasing prevalence of antibiotic-resistant Shigella sp. emphasizes that alternatives to conventional antibiotics are needed. Siphoviridae bacteriophage (phage), pSf-2, infecting S. flexneri ATCC(®) 12022 was isolated from Geolpocheon stream in Korea. Morphological analysis by transmission electron microscopy revealed that pSf-2 has a head of about 57 ± 4 nm in diameter with a long tail of 136 ± 3 nm in length and 15 ± 2 nm in width. One-step growth analysis revealed that pSf-2 has latent period of 30 min and burst size of 16 PFU/infected cell. The DNA genome of pSf-2 is composed of 50,109 bp with a G+C content of 45.44 %. The genome encodes 83 putative ORFs, 19 putative promoters, and 23 transcriptional terminator regions. Genome sequence analysis of pSf-2 and comparative analysis with the homologous T1-like Shigella phages, Shfl1 and pSf-1, revealed that pSf-2 is a novel T1-like Shigella phage. These results showed that pSf-2 might have a high potential as a biocontrol agent to control shigellosis. Also, the genomic information may lead to further understanding of phage biodiversity, especially T1-like phages.
authors.affiliation: College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, 151-742, South Korea.
authors.forename: Jin Woo
authors.initials: JW
authors.lastname: Jun
authors.affiliation: College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, 151-742, South Korea.
authors.forename: Hyoun Joong
authors.initials: HJ
authors.lastname: Kim
authors.affiliation: College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, 151-742, South Korea.
authors.forename: Sae Kil
authors.initials: SK
authors.lastname: Yun
authors.affiliation: Departments of Rheumatology, Bundang Jesaeng Hospital, Seongnam, 463-774, South Korea.
authors.forename: Ji Young
authors.initials: JY
authors.lastname: Chai
authors.affiliation: College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, 151-742, South Korea. bclee@snu.ac.kr.
authors.forename: Byeong Chun
authors.initials: BC
authors.lastname: Lee
authors.affiliation: College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, 151-742, South Korea. parksec@snu.ac.kr.
authors.forename: Se Chang
authors.initials: SC
authors.lastname: Park
mesh_terms: D001435:Bacteriophages / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification* / Q000648:ultrastructure; D001482:Base Composition; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D023061:Gene Order; D016679:Genome, Viral; D007723:Korea; D046529:Microscopy, Electron, Transmission; D016366:Open Reading Frames; D045483:Rivers / Q000821:virology; D017422:Sequence Analysis, DNA; D012760:Shigella / Q000821:virology*; D017906:Siphoviridae / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification* / Q000648:ultrastructure
chemical_list: D004279:DNA, Viral