title: Characterization of oleaginous yeasts accumulating high levels of lipid when cultivated in glycerol and their potential for lipid production from biodiesel-derived crude glycerol.
abstract: This study attempted to identify oleaginous yeasts and selected the strain that accumulated the largest quantity of lipid for lipid production from glycerol. Two-step screening of 387 yeast strains revealed 23 oleaginous strains that accumulated quantities of lipid higher than 20 % of their biomass when cultivated in glycerol. These strains were identified to be four ascomycetous yeast species i.e. Candida silvae, Kodamaea ohmeri, Meyerozyma caribbica, and Pichia manshurica, and five basidiomycetous yeast species i.e. Cryptococcus cf. podzolicus, Cryptococcus laurentii, Rhodosporidium fluviale, Rhodotorula taiwanensis, and Sporidiobolus ruineniae. Rhodosporidium fluviale DMKU-RK253 accumulated the highest quantity of lipid equal to 65.2 % of its biomass (3.9 g L(-1) lipid and 6.0 g L(-1) biomass) by shaking flask cultivation in crude glycerol. The main fatty acids in the accumulated lipid of this strain consisted of oleic acid, linoleic acid, and palmitic acid. Therefore, R. fluviale DMKU-RK253 has potential for producing lipid for biodiesel manufacturing using crude glycerol as a feedstock.
authors.affiliation: Department of Microbiology, Faculty of Science, Kasetsart University, Chatuchak, Bangkok 10900, Thailand.
authors.forename: Pirapan
authors.initials: P
authors.lastname: Polburee
authors.affiliation: Department of Microbiology, Faculty of Science, Kasetsart University, Chatuchak, Bangkok 10900, Thailand.
authors.forename: Wichien
authors.initials: W
authors.lastname: Yongmanitchai
authors.affiliation: Department of Microbiology, Faculty of Science, Kasetsart University, Chatuchak, Bangkok 10900, Thailand.
authors.forename: Noppon
authors.initials: N
authors.lastname: Lertwattanasakul
authors.affiliation: International Center for Biotechnology, Osaka University, Suita-shi, Osaka 565-0871, Japan.
authors.forename: Takao
authors.initials: T
authors.lastname: Ohashi
authors.affiliation: International Center for Biotechnology, Osaka University, Suita-shi, Osaka 565-0871, Japan.
authors.forename: Kazuhito
authors.initials: K
authors.lastname: Fujiyama
authors.affiliation: Department of Microbiology, Faculty of Science, Kasetsart University, Chatuchak, Bangkok 10900, Thailand; Center for Advanced Studied in Tropical Natural Resources, National Research University-Kasetsart University, Chatuchak, Bangkok 10900, Thailand. Electronic address: fscistl@ku.ac.th.
authors.forename: Savitree
authors.initials: S
authors.lastname: Limtong
mesh_terms: D001487:Basidiomycota / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D056804:Biofuels / Q000032:analysis / Q000382:microbiology*; D018533:Biomass; D001709:Biotechnology; D003470:Culture Media / Q000032:analysis / Q000378:metabolism; D005990:Glycerol / Q000737:chemistry / Q000378:metabolism*; D050356:Lipid Metabolism; D015003:Yeasts / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D056804:Biofuels; D003470:Culture Media; D005990:Glycerol
keywords: Biodiversity; Microbial lipid; Rhodosporidium fluviale; Waste glycerol