title: RT-PCR and sequence analysis of the full-length fusion protein of Canine Distemper Virus from domestic dogs.
abstract: During 2007-2014, 84 out of 236 (35.6%) samples from domestic dogs submitted to our laboratory for diagnostic purposes were positive for Canine Distemper Virus (CDV), as analyzed by RT-PCR amplification of a fragment of the nucleoprotein gene. Fifty-nine of them (70.2%) were from dogs that had been vaccinated against CDV. The full-length gene encoding the Fusion (F) protein of fifteen isolates was sequenced and compared with that of those of other CDVs, including wild-type and vaccine strains. Phylogenetic analysis using the F gene full-length sequences grouped all the Argentinean CDV strains in the SA2 clade. Sequence identity with the Onderstepoort vaccine strain was 89.0-90.6%, and the highest divergence was found in the 135 amino acids corresponding to the F protein signal-peptide, Fsp (64.4-66.7% identity). In contrast, this region was highly conserved among the local strains (94.1-100% identity). One extra putative N-glycosylation site was identified in the F gene of CDV Argentinean strains with respect to the vaccine strain. The present report is the first to analyze full-length F protein sequences of CDV strains circulating in Argentina, and contributes to the knowledge of molecular epidemiology of CDV, which may help in understanding future disease outbreaks.
authors.affiliation: Centro de Virología Animal (CEVAN), Instituto de Ciencia y Tecnología Dr. César Milstein, CONICET, Saladillo 2468, C1440FFX, Ciudad de Buenos Aires, Argentina.
authors.forename: Carina
authors.initials: C
authors.lastname: Romanutti
authors.affiliation: Centro de Virología Animal (CEVAN), Instituto de Ciencia y Tecnología Dr. César Milstein, CONICET, Saladillo 2468, C1440FFX, Ciudad de Buenos Aires, Argentina.
authors.forename: Marina
authors.initials: M
authors.lastname: Gallo Calderón
authors.affiliation: Centro de Virología Animal (CEVAN), Instituto de Ciencia y Tecnología Dr. César Milstein, CONICET, Saladillo 2468, C1440FFX, Ciudad de Buenos Aires, Argentina.
authors.forename: Leticia
authors.initials: L
authors.lastname: Keller
authors.affiliation: Centro de Virología Animal (CEVAN), Instituto de Ciencia y Tecnología Dr. César Milstein, CONICET, Saladillo 2468, C1440FFX, Ciudad de Buenos Aires, Argentina. Electronic address: nmattioncevan@centromilstein.org.ar.
authors.forename: Nora
authors.initials: N
authors.lastname: Mattion
authors.affiliation: Centro de Virología Animal (CEVAN), Instituto de Ciencia y Tecnología Dr. César Milstein, CONICET, Saladillo 2468, C1440FFX, Ciudad de Buenos Aires, Argentina.
authors.forename: José
authors.initials: J
authors.lastname: La Torre
mesh_terms: D000818:Animals; D001118:Argentina / Q000453:epidemiology; D004196:Disease Outbreaks / Q000517:prevention & control / Q000662:veterinary; D004216:Distemper / Q000453:epidemiology / Q000821:virology; D004217:Distemper Virus, Canine / Q000235:genetics*; D004285:Dogs; D005838:Genotype; D057805:Pets / Q000821:virology*; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014760:Viral Fusion Proteins / Q000737:chemistry / Q000235:genetics*
chemical_list: D012367:RNA, Viral; D014760:Viral Fusion Proteins; C076619:F protein, Phocine distemper virus
keywords: Canine Distemper Virus; F gene; Phylogenetic analysis; RT-PCR