title: Genome sequence of Bradyrhizobium sp. WSM1253; a microsymbiont of Ornithopus compressus from the Greek Island of Sifnos.
abstract: Bradyrhizobium sp. WSM1253 is a novel N2-fixing bacterium isolated from a root nodule of the herbaceous annual legume Ornithopus compressus that was growing on the Greek Island of Sifnos. WSM1253 emerged as a strain of interest in an Australian program that was selecting inoculant quality bradyrhizobial strains for inoculation of Mediterranean species of lupins (Lupinus angustifolius, L. princei, L. atlanticus, L. pilosus). In this report we describe, for the first time, the genome sequence information and annotation of this legume microsymbiont. The 8,719,808 bp genome has a G + C content of 63.09 % with 71 contigs arranged into two scaffolds. The assembled genome contains 8,432 protein-coding genes, 66 RNA genes and a single rRNA operon. This improved-high-quality draft rhizobial genome is one of 20 sequenced through a DOE Joint Genome Institute 2010 Community Sequencing Project.
authors.affiliation: Centre for Rhizobium Studies, Murdoch University, Murdoch, Australia.
authors.forename: Ravi
authors.initials: R
authors.lastname: Tiwari
authors.affiliation: Centre for Rhizobium Studies, Murdoch University, Murdoch, Australia.
authors.forename: John
authors.initials: J
authors.lastname: Howieson
authors.affiliation: Centre for Rhizobium Studies, Murdoch University, Murdoch, Australia ; Department of Agriculture and Food, South Perth, Australia.
authors.forename: Ron
authors.initials: R
authors.lastname: Yates
authors.affiliation: Centre for Rhizobium Studies, Murdoch University, Murdoch, Australia.
authors.forename: Rui
authors.initials: R
authors.lastname: Tian
authors.affiliation: Los Alamos National Laboratory, Bioscience Division, Los Alamos, NM USA.
authors.forename: Britanny
authors.initials: B
authors.lastname: Held
authors.affiliation: Los Alamos National Laboratory, Bioscience Division, Los Alamos, NM USA.
authors.forename: Roxanne
authors.initials: R
authors.lastname: Tapia
authors.affiliation: Los Alamos National Laboratory, Bioscience Division, Los Alamos, NM USA.
authors.forename: Cliff
authors.initials: C
authors.lastname: Han
authors.affiliation: DOE Joint Genome Institute, Walnut Creek, CA USA.
authors.forename: Rekha
authors.initials: R
authors.lastname: Seshadri
authors.affiliation: DOE Joint Genome Institute, Walnut Creek, CA USA.
authors.forename: T B K
authors.initials: TB
authors.lastname: Reddy
authors.affiliation: DOE Joint Genome Institute, Walnut Creek, CA USA.
authors.forename: Marcel
authors.initials: M
authors.lastname: Huntemann
authors.affiliation: DOE Joint Genome Institute, Walnut Creek, CA USA.
authors.forename: Amrita
authors.initials: A
authors.lastname: Pati
authors.affiliation: DOE Joint Genome Institute, Walnut Creek, CA USA.
authors.forename: Tanja
authors.initials: T
authors.lastname: Woyke
authors.affiliation: Biological Data Management and Technology Center, Lawrence Berkeley National Laboratory, Berkeley, CA USA.
authors.forename: Victor
authors.initials: V
authors.lastname: Markowitz
authors.affiliation: DOE Joint Genome Institute, Walnut Creek, CA USA.
authors.forename: Natalia
authors.initials: N
authors.lastname: Ivanova
authors.affiliation: DOE Joint Genome Institute, Walnut Creek, CA USA ; Department of Biological Sciences, King Abdulaziz University, Jeddah, Saudi Arabia.
authors.forename: Nikos
authors.initials: N
authors.lastname: Kyrpides
authors.affiliation: Centre for Rhizobium Studies, Murdoch University, Murdoch, Australia.
authors.forename: Wayne
authors.initials: W
authors.lastname: Reeve
keywords: Ornithopus; nitrogen fixation; rhizobia; root-nodule bacteria