title: Lachnotalea glycerinigen. nov., sp. nov., an anaerobe isolated from a nanofiltration unit treating anoxic groundwater.
abstract: A strictly anaerobic bacterium, strain DLD10T, was isolated from a biofilm that developed on a nanofiltration membrane treating anoxic groundwater using glycerol as substrate. Cells were straight to slightly curved rods 0.2-0.5 μm in diameter and 1-3 μm in length, non-motile and non-spore-forming. The optimum temperature and pH for growth were 30 °C and pH 7.0. Strain DLD10Twas able to grow in the presence of 0.03-4.5 % (w/v) NaCl. Substrates utilized by strain DLD10Tincluded glycerol and various carbohydrates (glucose, sucrose, fructose, mannose, arabinose, pectin, starch, xylan), which were mainly converted to ethanol, acetate, H2and formate. Thiosulphate, sulphur and Fe(III) were used as electron acceptors, but sulphate, fumarate and nitrate were not. The predominant membrane fatty acids were C16 : 0, iso-C17 : 1and C17 : 1ω8c. The DNA G+C content was 36.4 mol%. Strain DLD10Tbelongs to the familyLachnospiraceaeand is distantly related toClostridium populetiDSM 5832T,Hespellia porcinaDSM 15481TandRobinsoniella peoriensisCCUG 48729T(93 % 16S rRNA gene sequence similarity). Physiological characteristics and phylogenetic analysis indicated that strain DLD10Tis a representative of a novel species of a new genus, for which the nameLachnotalea glycerinigen. nov., sp. nov. is proposed. The type strain ofLachnotalea glyceriniis DLD10T( = DSM 28816T= JCM 30818T).
authors.affiliation: Wetsus, European Centre of Excellence for Sustainable Water Technology, Oostergoweg 9, 8911 MA Leeuwarden, The Netherlands.|Laboratory of Microbiology, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, The Netherlands.
authors.forename: Monika
authors.initials: M
authors.lastname: Jarzembowska
authors.affiliation: Laboratory of Microbiology, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, The Netherlands.
authors.forename: Diana Z
authors.initials: DZ
authors.lastname: Sousa
authors.affiliation: Laboratory of Microbiology, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, The Netherlands.|Wetsus, European Centre of Excellence for Sustainable Water Technology, Oostergoweg 9, 8911 MA Leeuwarden, The Netherlands.
authors.forename: Florian
authors.initials: F
authors.lastname: Beyer
authors.affiliation: Wetsus, European Centre of Excellence for Sustainable Water Technology, Oostergoweg 9, 8911 MA Leeuwarden, The Netherlands.
authors.forename: Arie
authors.initials: A
authors.lastname: Zwijnenburg
authors.affiliation: Laboratory of Microbiology, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, The Netherlands.|Wetsus, European Centre of Excellence for Sustainable Water Technology, Oostergoweg 9, 8911 MA Leeuwarden, The Netherlands.
authors.forename: Caroline M
authors.initials: CM
authors.lastname: Plugge
authors.affiliation: Laboratory of Microbiology, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, The Netherlands.
authors.forename: Alfons J M
authors.initials: AJM
authors.lastname: Stams