title: Prevalence, Clinical Criteria and Sociodemographic Predictors of Trichomonas vaginalis Infection in Suspected Egyptian Women, Using Direct Diagnostic Techniques.
abstract: BACKGROUND
  The present study aimed to determine the prevalence and associated risk factors of vaginal trichomoniasis in women referred to gynecologic clinic in Benha University Hospital, Egypt.
  
  
  METHODS
  Two hundred female patients enrolled in the study. Vaginal samples were obtained from them and examined for T. vaginalis by wet mount, Giemsa stain, Acridine orange (AO) stain and culture on modified Diamond's medium. For analysis of accuracy of the methods used, the receiver operating characteristic (ROC) curve concept with culture as a gold standard was applied.
  
  
  RESULTS
  Out of 200 patients, T. vaginalis was found in 22 (11%) patients by any of the diagnostic methods used. The accuracy of AO staining comes next to Diamond's culture (AUC 0.909, sensitivity 81.8%, specificity 100%, CI 0.81-1.0) followed by Giemsa staining (AUC 0.835, sensitivity 68.2%, specificity 98.9%, CI 0.72-0.95). The wet mount was the least accurate method (AUC 0.795, sensitivity 59.1%, specificity 100%, CI 0.67-0.92). There was no significant association between potentially supposed risk factors and trichomoniasis except patients complaining of either dysuria and dyspareunia or back pain and abdominal pain.
  
  
  CONCLUSIONS
  Trichomoniasis is a common disease in our community. Sociodemographic factors do not seem to affect the prevalence among different Egyptian population. For accurate diagnosis, laboratory investigation is essential. A positive wet smear is diagnostic, but negative samples should be examined by methods that are more sensitive.
authors.affiliation: Department of Parasitology, Behna Faculty of Medicine, Behna University, Benha, Egypt.
authors.forename: Atef Hussein
authors.initials: AH
authors.lastname: Hussein
authors.affiliation: Department of Parasitology, Behna Faculty of Medicine, Behna University, Benha, Egypt.
authors.forename: Mohamed Hussein
authors.initials: MH
authors.lastname: Saleh
authors.affiliation: Department of Parasitology, Behna Faculty of Medicine, Behna University, Benha, Egypt.
authors.forename: Ibrahim Maged
authors.initials: IM
authors.lastname: Nagaty
authors.affiliation: Department of Parasitology, Behna Faculty of Medicine, Behna University, Benha, Egypt.
authors.forename: Khaled
authors.initials: K
authors.lastname: A Ghieth
authors.affiliation: Department of Parasitology, Behna Faculty of Medicine, Behna University, Benha, Egypt.
authors.forename: Nagat Ahmed
authors.initials: NA
authors.lastname: El-Azab
keywords: Acridine orange; Diamond’s culture; Trichomonas vaginalis; Wet mount