title: High-Throughput DNA Array for SNP Detection of KRAS Gene Using a Centrifugal Microfluidic Device.
abstract: Here, we describe detection of single nucleotide polymorphism (SNP) in genomic DNA samples using a NanoBioArray (NBA) chip. Fast DNA hybridization is achieved in the chip when target DNAs are introduced to the surface-arrayed probes using centrifugal force. Gold nanoparticles (AuNPs) are used to assist SNP detection at room temperature. The parallel setting of sample introduction in the spiral channels of the NBA chip enables multiple analyses on many samples, resulting in a technique appropriate for high-throughput SNP detection. The experimental procedure, including chip fabrication, probe array printing, DNA amplification, hybridization, signal detection, and data analysis, is described in detail.
authors.affiliation: Department of Chemistry, Simon Fraser University, 8888 University Drive, Burnaby, British Columbia, Canada, V5A 1S6.
authors.forename: Abootaleb
authors.initials: A
authors.lastname: Sedighi
authors.affiliation: Department of Chemistry, Simon Fraser University, 8888 University Drive, Burnaby, British Columbia, Canada, V5A 1S6. Paulli@sfu.ca.
authors.forename: Paul C H
authors.initials: PC
authors.lastname: Li
mesh_terms: D000447:Aldehydes; D002498:Centrifugation; D015342:DNA Probes / Q000737:chemistry / Q000235:genetics; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D011905:Genes, ras / Q000235:genetics*; D006046:Gold / Q000737:chemistry; D056656:Lab-On-A-Chip Devices; D053768:Metal Nanoparticles / Q000737:chemistry; D021141:Nucleic Acid Amplification Techniques; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation*; D020641:Polymorphism, Single Nucleotide; D013050:Spectrometry, Fluorescence; D013499:Surface Properties; D013696:Temperature
chemical_list: D000447:Aldehydes; D015342:DNA Probes; D004129:Dimethylpolysiloxanes; C013830:baysilon; D006046:Gold
keywords: DNA microarray; Gold nanoparticles; Microfluidics; NanoBioArray (NBA ); PDMS chip; Single nucleotide polymorphism (SNP )