title: [The expression and role of the transcription factor C/EBPα in chronic myeloid leukemia].
abstract: OBJECTIVE
  To investigate the expression and the possible mechanism of the transcription factor C/EBPα in chronic myeloid leukemia（CML).
  
  
  METHODS
  Bone marrow samples from 50 CML patients（including 33 patients in chronic phase, 7 in accelerated phase and 10 in blast crisis）and peripheral blood specimens of 20 healthy donors were collected. The expression of C/EBPα gene and the effect of Imatinib on its expression was detected by RT- PCR. C/EBPα gene was inserted into lentivirus expression vector pLVX- EGFP- 3FLAG- Puro by recombinant DNA technology to construct C/EBPα stable expression in K562 cells. Cell proliferation was assayed by CCK-8. The expressions of Foxo3a and Bim genes were detected by RT-PCR.
  
  
  RESULTS
  The level of C/EBPα expression was significantly declined in CML patients compared with that of normal control group（P<0.01）and had negative correlation with bcr- abl expression（Spearman r=－ 0.505, P<0.01). The stable K562- C/EBPα cell line was successfully established and confirmed by RT-PCR and Western blot. Cell proliferation ability was lower in the K562- C/EBPα group than that in the non- transfection and mock-vehicle groups. The expressions of Foxo3a and Bim genes were 1.06 ± 0.06 and 0.53 ± 0.07, respectively, which was higher than that of nontransfection and mock-vehicle groups（P<0.01, P<0.05).
  
  
  CONCLUSIONS
  C/EBPα expression was decreased in CML patients, overexpression of C/EBPα could inhibit K562 cell growth.
authors.affiliation: Department of Laboratory, Yantai Yuhuangding Hospital, Medical College, Qingdao University, Yantai 264000, China.
authors.forename: Guili
authors.initials: G
authors.lastname: Zhang
authors.affiliation: Department of Laboratory, Yantai Yuhuangding Hospital, Medical College, Qingdao University, Yantai 264000, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Dong
authors.affiliation: Department of Laboratory, Yantai Yuhuangding Hospital, Medical College, Qingdao University, Yantai 264000, China.
authors.forename: Caifu
authors.initials: C
authors.lastname: Luan
authors.affiliation: Department of Laboratory, Yantai Yuhuangding Hospital, Medical College, Qingdao University, Yantai 264000, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Laboratory, Yantai Yuhuangding Hospital, Medical College, Qingdao University, Yantai 264000, China.
authors.forename: Huiyuan
authors.initials: H
authors.lastname: Shao
authors.affiliation: Department of Laboratory, Yantai Yuhuangding Hospital, Medical College, Qingdao University, Yantai 264000, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Laboratory, Yantai Yuhuangding Hospital, Medical College, Qingdao University, Yantai 264000, China.
authors.forename: Chengming
authors.initials: C
authors.lastname: Sun
mesh_terms: D001752:Blast Crisis; D001853:Bone Marrow; D022763:CCAAT-Enhancer-Binding Protein-alpha / Q000378:metabolism*; D016022:Case-Control Studies; D002453:Cell Cycle; D049109:Cell Proliferation; D006801:Humans; D000068877:Imatinib Mesylate; D020014:K562 Cells; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000378:metabolism*; D014162:Transfection
chemical_list: D022763:CCAAT-Enhancer-Binding Protein-alpha; D000068877:Imatinib Mesylate