title: Primary intraosseous squamous cell carcinoma arising in an odontogenic keratocyst previously treated with marsupialization: case report and immunohistochemical study.
abstract: A rare case of primary intraosseous squamous cell carcinoma arising in an odontogenic keratocyst (OKC) is presented here, with the clinical and histologic features of the first biopsy showing characteristics of OKC and the second biopsy disclosing a squamous cell carcinoma. Immunoprofile of this case was compared with five cases of classical OKC by using cytokeratins CK5, CK14, and CK19, CD138, p63, Ki-67, p53, and bcl-2. Classic OKCs showed expected positivity, mainly in the basal and/or suprabasal layers with most antibodies, except for p53, which was negative, whereas the present case showed irregular positivity in all layers, indicating that this can be useful for differential diagnosis and suggesting a possible role in malignant transformation into primary intraosseous squamous cell carcinoma. In conclusion, immunohistochemical differences between the first biopsy of the present case and classic OKC suggest that immunohistochemistry can be helpful in cases with areas of subtle initial malignant transformation.
authors.affiliation: Oral Pathology Section, Department of Oral Diagnosis, Piracicaba Dental School, University of Campinas (UNICAMP), Piracicaba, São Paulo, Brazil. Electronic address: Cdmarisolmtz@gmail.com.
authors.forename: Marisol
authors.initials: M
authors.lastname: Martínez-Martínez
authors.affiliation: Department of Health Care, Universidad Autónoma Metropolitana, Xochimilco, México.
authors.forename: Adalberto
authors.initials: A
authors.lastname: Mosqueda-Taylor
authors.affiliation: Department of Oral Pathology, Oral Medicine and Oral Surgery, Faculty of Dentistry, Universidad Peruana Ceyetano Heredia, Lima, Peru.
authors.forename: Wilson
authors.initials: W
authors.lastname: Delgado-Azañero
authors.affiliation: Oral Pathology Section, Department of Oral Diagnosis, Piracicaba Dental School, University of Campinas (UNICAMP), Piracicaba, São Paulo, Brazil.
authors.forename: Alicia
authors.initials: A
authors.lastname: Rumayor-Piña
authors.affiliation: Oral Pathology Section, Department of Oral Diagnosis, Piracicaba Dental School, University of Campinas (UNICAMP), Piracicaba, São Paulo, Brazil.
authors.forename: Oslei Paes
authors.initials: OP
authors.lastname: de Almeida
mesh_terms: D000328:Adult; D001706:Biopsy; D002294:Carcinoma, Squamous Cell / Q000000981:diagnostic imaging / Q000473:pathology* / Q000601:surgery; D002471:Cell Transformation, Neoplastic / Q000473:pathology*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008339:Mandibular Neoplasms / Q000000981:diagnostic imaging / Q000473:pathology* / Q000601:surgery; D009807:Odontogenic Cysts / Q000000981:diagnostic imaging / Q000473:pathology* / Q000601:surgery; D011862:Radiography, Panoramic