title: Expression of mitochondria-related genes is elevated in overfeeding-induced goose fatty liver.
abstract: Mitochondrion, the power house of the cell, is an important organelle involving in energy homeostasis. Change in mitochondrial mass and function may lead to metabolic disorders. Previous studies indicate that mitochondrial mass loss and dysfunction are associated with non-alcoholic fatty liver disease (NAFLD) in human and mouse. However, it is unclear whether mitochondrial genes are involved in the development of goose fatty liver. To address this, we determined the response of goose mitochondrial genes to overfeeding and other fatty liver-related factors (e.g., hyperinsulinemia, hyperglycemia, and hyperlipidemia). We first employed RNA-seq technology to determine the differentially expressed genes in the livers from normally-fed vs. overfed geese, followed by bioinformatics analysis and quantitative PCR validation. Data indicated that a majority of mitochondrial genes in the liver were induced by overfeeding. To understand how these genes are regulated in the context of fatty liver, we treated goose primary hepatocytes with high levels of glucose, fatty acids and insulin. The results indicated that these factors had an influence on the expression of some mitochondria related genes. Together, these findings suggest that the induction of mitochondrial gene expression by overfeeding is required for the development of goose fatty liver, and this induction is partially attributable to hyperglycemia, hyperlipidemia and hyperinsulinemia.
authors.affiliation: College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu 225009, China; College of Veterinary Science, West Kordofan University, El Nuhud 20, Sudan.
authors.forename: Rashid H
authors.initials: RH
authors.lastname: Osman
authors.affiliation: College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu 225009, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Shao
authors.affiliation: College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu 225009, China.
authors.forename: Long
authors.initials: L
authors.lastname: Liu
authors.affiliation: College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu 225009, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Xia
authors.affiliation: College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu 225009, China.
authors.forename: Xiaoxian
authors.initials: X
authors.lastname: Sun
authors.affiliation: College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu 225009, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu 225009, China.
authors.forename: Laidi
authors.initials: L
authors.lastname: Wang
authors.affiliation: College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu 225009, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu 225009, China.
authors.forename: Yihui
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu 225009, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu 225009, China. Electronic address: yzgong@163.com.
authors.forename: Daoqing
authors.initials: D
authors.lastname: Gong
authors.affiliation: College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu 225009, China. Electronic address: tygeng@yzu.edu.cn.
authors.forename: Tuoyu
authors.initials: T
authors.lastname: Geng
mesh_terms: D000818:Animals; D059305:Diet, High-Fat / Q000009:adverse effects; D004041:Dietary Fats / Q000009:adverse effects; D005227:Fatty Acids / Q000494:pharmacology; D005234:Fatty Liver / Q000209:etiology / Q000235:genetics* / Q000473:pathology / Q000662:veterinary*; D005777:Geese; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D005947:Glucose / Q000494:pharmacology; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D006943:Hyperglycemia / Q000473:pathology; D006946:Hyperinsulinism / Q000473:pathology; D006949:Hyperlipidemias / Q000473:pathology; D007328:Insulin / Q000494:pharmacology; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D024101:Mitochondrial Proteins / Q000235:genetics* / Q000378:metabolism; D058977:Molecular Sequence Annotation; D011201:Poultry Diseases / Q000209:etiology / Q000235:genetics* / Q000473:pathology; D061251:Primary Cell Culture; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism
chemical_list: D004041:Dietary Fats; D005227:Fatty Acids; D007328:Insulin; D024101:Mitochondrial Proteins; D012333:RNA, Messenger; D005947:Glucose
keywords: Fatty liver; Goose; Mitochondrion; Overfeeding