title: Supportless electrochemical sensor based on molecularly imprinted polymer modified nanoporous microrod for determination of dopamine at trace level.
abstract: In this work, we developed a novel freestanding metallic microrod as working electrode for highly sensitive and selective electrochemical detection of trace dopamine (DA). The electrode was facilely fabricated via first dealloying smooth Au-Ag alloy microrod (AMR) into nanoporous Au-Ag alloy microrod (NPAMR) and further modifying with electro-polymerized molecularly imprinted polymer (MIP). Influencing factors during electro-polymerization process including pH value and molar ratio of monomer to template molecule were optimized. Under the optimal conditions, a linear range from 2 × 10(-13) to 2 × 10(-8)M for measuring DA was obtained with an ultralow detection limit of 7.63 × 10(-14)M (S/N=3). In addition, the MIP-modified electrode (MIP/NPAMR) was successfully employed to test DA in serum and brain samples.
authors.affiliation: Key Laboratory of Xinjiang Phytomedicine Resources of Ministry of Education, School of Pharmacy, Shihezi University, Shihezi 832000, PR China; Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan, School of Chemistry and Chemical Engineering, Shihezi University, Shihezi 832003, PR China. Electronic address: yingchunli@shzu.edu.cn.
authors.forename: Yingchun
authors.initials: Y
authors.lastname: Li
authors.affiliation: Key Laboratory of Xinjiang Phytomedicine Resources of Ministry of Education, School of Pharmacy, Shihezi University, Shihezi 832000, PR China.
authors.forename: Han
authors.initials: H
authors.lastname: Song
authors.affiliation: Key Laboratory of Xinjiang Phytomedicine Resources of Ministry of Education, School of Pharmacy, Shihezi University, Shihezi 832000, PR China.
authors.forename: Lu
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Lab of Biosystems and Microanalysis, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, PR China.
authors.forename: Peng
authors.initials: P
authors.lastname: Zuo
authors.affiliation: Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan, School of Chemistry and Chemical Engineering, Shihezi University, Shihezi 832003, PR China; Lab of Biosystems and Microanalysis, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, PR China.
authors.forename: Bang-Ce
authors.initials: BC
authors.lastname: Ye
authors.affiliation: School of Pharmacy, Xinjiang Medical University, Urumqi, Xinjiang 830011, PR China. Electronic address: xydyaojun@163.com.
authors.forename: Jun
authors.initials: J
authors.lastname: Yao
authors.affiliation: Key Laboratory of Xinjiang Phytomedicine Resources of Ministry of Education, School of Pharmacy, Shihezi University, Shihezi 832000, PR China. Electronic address: cw_pha@shzu.edu.cn.
authors.forename: Wen
authors.initials: W
authors.lastname: Chen
mesh_terms: D015374:Biosensing Techniques; D004298:Dopamine / Q000737:chemistry / Q000302:isolation & purification*; D055664:Electrochemical Techniques; D006046:Gold / Q000737:chemistry; D006801:Humans; D057230:Limit of Detection; D011108:Polymers / Q000737:chemistry
chemical_list: D011108:Polymers; D006046:Gold; D004298:Dopamine
keywords: Dopamine; Electrochemical sensor; Molecularly imprinted polymer; Nanoporous alloy microrod; Trace measurement