title: Redox State of Cytochromes in Frozen Yeast Cells Probed by Resonance Raman Spectroscopy.
abstract: Cryopreservation is a well-established technique used for the long-term storage of biological materials whose biological activity is effectively stopped under low temperatures (suspended animation). Since most biological methods do not work in a low-temperature frozen environment, the mechanism and details of the depression of cellular activity in the frozen state remain largely uncharacterized. In this work, we propose, to our knowledge, a new approach to study the downregulation of the redox activity of cytochromes b and c in freezing yeast cells in a contactless, label-free manner. Our approach is based on cytochrome photobleaching effects observed in the resonance Raman spectra of live cells. Photoinduced and native redox reactions that contributed to the photobleaching rate were studied over a wide temperature range (from -173 to +25 °C). We found that ice formation influences both the rate of cytochrome redox reactions and the balance between the reduced and oxidized cytochromes. We demonstrate that the temperature dependence of native redox reaction rates can be well described by the thermal activation law with an apparent energy of 32.5 kJ/mol, showing that the redox reaction rate is ∼10(15) times slower at liquid nitrogen temperature than at room temperature.
authors.affiliation: Institute of Automation and Electrometry, Russian Academy of Sciences, Novosibirsk, Russia.
authors.forename: Konstantin A
authors.initials: KA
authors.lastname: Okotrub
authors.affiliation: Institute of Automation and Electrometry, Russian Academy of Sciences, Novosibirsk, Russia; Novosibirsk State University, Novosibirsk, Russia. Electronic address: lab21@iae.nsk.su.
authors.forename: Nikolay V
authors.initials: NV
authors.lastname: Surovtsev
mesh_terms: D015925:Cryopreservation; D045303:Cytochromes b / Q000378:metabolism*; D045304:Cytochromes c / Q000378:metabolism*; D004579:Electron Transport; D010084:Oxidation-Reduction; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000378:metabolism; D013059:Spectrum Analysis, Raman; D013696:Temperature
chemical_list: D045304:Cytochromes c; D045303:Cytochromes b