title: Changes of protein glycosylation in the course of radiotherapy.
abstract: This is the first study of changes in protein glycosylation due to exposure of human subjects to ionizing radiation. Site specific glycosylation patterns of 7 major plasma proteins were analyzed; 171 glycoforms were identified; and the abundance of 99 of these was followed in the course of cancer radiotherapy in 10 individual patients. It was found that glycosylation of plasma proteins does change in response to partial body irradiation (∼ 60 Gy), and the effects last during follow-up; the abundance of some glycoforms changed more than twofold. Both the degree of changes and their time-evolution showed large inter-individual variability.
authors.affiliation: MS Proteomics Research Group, Institute of Organic Chemistry, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Budapest, Hungary; Semmelweis University, School of Ph.D. Studies, Budapest, Hungary.
authors.forename: Eszter
authors.initials: E
authors.lastname: Tóth
authors.affiliation: Core Technologies Centre, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Budapest, Hungary.
authors.forename: Károly
authors.initials: K
authors.lastname: Vékey
authors.affiliation: MS Proteomics Research Group, Institute of Organic Chemistry, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Budapest, Hungary.
authors.forename: Olivér
authors.initials: O
authors.lastname: Ozohanics
authors.affiliation: MS Proteomics Research Group, Institute of Organic Chemistry, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Budapest, Hungary.
authors.forename: Anita
authors.initials: A
authors.lastname: Jekő
authors.affiliation: Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice Branch, Gliwice, Poland.
authors.forename: Iwona
authors.initials: I
authors.lastname: Dominczyk
authors.affiliation: Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice Branch, Gliwice, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Widlak
authors.affiliation: MS Proteomics Research Group, Institute of Organic Chemistry, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Budapest, Hungary. Electronic address: drahos.laszlo@ttk.mta.hu.
authors.forename: László
authors.initials: L
authors.lastname: Drahos
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000595:Amino Acid Sequence; D001798:Blood Proteins / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects*; D005260:Female; D006031:Glycosylation / Q000528:radiation effects; D006258:Head and Neck Neoplasms / Q000097:blood* / Q000235:genetics / Q000532:radiotherapy*; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data
chemical_list: D001798:Blood Proteins
keywords: Glycosylation; Glycosylation pattern; Head and neck cancer; Proteomics; Radiotherapy