title: Coxsackievirus B4 Can Infect Human Peripheral Blood-Derived Macrophages.
abstract: Beyond acute infections, group B coxsackieviruses (CVB) are also reported to play a role in the development of chronic diseases, like type 1 diabetes. The viral pathogenesis mainly relies on the interplay between the viruses and innate immune response in genetically-susceptible individuals. We investigated the interaction between CVB4 and macrophages considered as major players in immune response. Monocyte-derived macrophages (MDM) generated with either M-CSF or GM-CSF were inoculated with CVB4, and infection, inflammation, viral replication and persistence were assessed. M-CSF-induced MDM, but not GM-CSF-induced MDM, can be infected by CVB4. In addition, enhancing serum was not needed to infect MDM in contrast with parental monocytes. The expression of viral receptor (CAR) mRNA was similar in both M-CSF and GM-CSF MDM. CVB4 induced high levels of pro-inflammatory cytokines (IL-6 and TNFα) in both MDM populations. CVB4 effectively replicated and persisted in M-CSF MDM, but IFNα was produced in the early phase of infection only. Our results demonstrate that CVB4 can replicate and persist in MDM. Further investigations are required to determine whether the interaction between the virus and MDM plays a role in the pathogenesis of CVB-induced chronic diseases.
authors.affiliation: Laboratoire de virologie EA3610, Faculté de Médecine, Université de Lille, CHU de Lille 59037, France. enagnonkazali.alidjinou@chru-lille.fr.
authors.forename: Enagnon Kazali
authors.initials: EK
authors.lastname: Alidjinou
authors.affiliation: Laboratoire de virologie EA3610, Faculté de Médecine, Université de Lille, CHU de Lille 59037, France. famara.sane@chru-lille.fr.
authors.forename: Famara
authors.initials: F
authors.lastname: Sané
authors.affiliation: Laboratoire d'immunologie, Faculté de Médecine, Université de Lille, CHU de Lille 59037, France. jacques.trauet@chru-lille.fr.
authors.forename: Jacques
authors.initials: J
authors.lastname: Trauet
authors.affiliation: Laboratoire d'anatomie pathologique, Faculté de Médecine, Université de Lille, CHU de Lille 59037, France. marie-christine.copin@chru-lille.fr.
authors.forename: Marie-Christine
authors.initials: MC
authors.lastname: Copin
authors.affiliation: Laboratoire de virologie EA3610, Faculté de Médecine, Université de Lille, CHU de Lille 59037, France. didier.hober@chru-lille.fr.
authors.forename: Didier
authors.initials: D
authors.lastname: Hober
mesh_terms: D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D029822:Enterovirus B, Human / Q000502:physiology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000378:metabolism; D054884:Host-Pathogen Interactions; D006801:Humans; D016173:Macrophage Colony-Stimulating Factor / Q000378:metabolism; D008264:Macrophages / Q000276:immunology / Q000821:virology*; D014779:Virus Replication
chemical_list: D016207:Cytokines; D016173:Macrophage Colony-Stimulating Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor
keywords: GM-CSF; J0101; M-CSF; coxsackievirus B; infection; inflammation; monocyte-derived macrophages; persistence