title: Development of Non-Cell Adhesive Vascular Grafts Using Supramolecular Building Blocks.
abstract: Cell-free approaches to in situ tissue engineering require materials that are mechanically stable and are able to control cell-adhesive behavior upon implantation. Here, the development of mechanically stable grafts with non-cell adhesive properties via a mix-and-match approach using ureido-pyrimidinone (UPy)-modified supramolecular polymers is reported. Cell adhesion is prevented in vitro through mixing of end-functionalized or chain-extended UPy-polycaprolactone (UPy-PCL or CE-UPy-PCL, respectively) with end-functionalized UPy-poly(ethylene glycol) (UPy-PEG) at a ratio of 90:10. Further characterization reveals intimate mixing behavior of UPy-PCL with UPy-PEG, but poor mechanical properties, whereas CE-UPy-PCL scaffolds are mechanically stable. As a proof-of-concept for the use of non-cell adhesive supramolecular materials in vivo, electrospun vascular scaffolds are applied in an aortic interposition rat model, showing reduced cell infiltration in the presence of only 10% of UPy-PEG. Together, these results provide the first steps toward advanced supramolecular biomaterials for in situ vascular tissue engineering with control over selective cell capturing.
authors.affiliation: Department of Biomedical Engineering, Laboratory of Chemical Biology, and Institute for Complex Molecular Systems, Eindhoven University of Technology, P.O. Box 513, 5600, MB, Eindhoven, The Netherlands.
authors.forename: Geert C
authors.initials: GC
authors.lastname: van Almen
authors.affiliation: Department of Cardio-Thoracic Surgery, University Medical Center Utrecht, 3584, CX, Utrecht, The Netherlands.
authors.forename: Hanna
authors.initials: H
authors.lastname: Talacua
authors.affiliation: Department of Biomedical Engineering, Laboratory of Chemical Biology, and Institute for Complex Molecular Systems, Eindhoven University of Technology, P.O. Box 513, 5600, MB, Eindhoven, The Netherlands.
authors.forename: Bastiaan D
authors.initials: BD
authors.lastname: Ippel
authors.affiliation: Department of Biomedical Engineering, Laboratory of Chemical Biology, and Institute for Complex Molecular Systems, Eindhoven University of Technology, P.O. Box 513, 5600, MB, Eindhoven, The Netherlands.
authors.forename: Björne B
authors.initials: BB
authors.lastname: Mollet
authors.affiliation: Department of Biomedical Engineering, Laboratory of Chemical Biology, and Institute for Complex Molecular Systems, Eindhoven University of Technology, P.O. Box 513, 5600, MB, Eindhoven, The Netherlands.
authors.forename: Mellany
authors.initials: M
authors.lastname: Ramaekers
authors.affiliation: Department of Biomedical Engineering, Soft Tissue Biomechanics and Tissue Engineering and Institute for Complex Molecular Systems, Eindhoven University of Technology, P.O. Box 513, 5600, MB, Eindhoven, The Netherlands.
authors.forename: Marc
authors.initials: M
authors.lastname: Simonet
authors.affiliation: Department of Biomedical Engineering, Soft Tissue Biomechanics and Tissue Engineering and Institute for Complex Molecular Systems, Eindhoven University of Technology, P.O. Box 513, 5600, MB, Eindhoven, The Netherlands.
authors.forename: Anthal I P M
authors.initials: AI
authors.lastname: Smits
authors.affiliation: Department of Biomedical Engineering, Soft Tissue Biomechanics and Tissue Engineering and Institute for Complex Molecular Systems, Eindhoven University of Technology, P.O. Box 513, 5600, MB, Eindhoven, The Netherlands.
authors.forename: Carlijn V C
authors.initials: CV
authors.lastname: Bouten
authors.affiliation: Department of Cardio-Thoracic Surgery, University Medical Center Utrecht, 3584, CX, Utrecht, The Netherlands.
authors.forename: Jolanda
authors.initials: J
authors.lastname: Kluin
authors.affiliation: Department of Biomedical Engineering, Laboratory of Chemical Biology, and Institute for Complex Molecular Systems, Eindhoven University of Technology, P.O. Box 513, 5600, MB, Eindhoven, The Netherlands.
authors.forename: Patricia Y W
authors.initials: PY
authors.lastname: Dankers
mesh_terms: D000818:Animals; D001705:Bioprosthesis; D001807:Blood Vessel Prosthesis; D002448:Cell Adhesion; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D051379:Mice; D041681:NIH 3T3 Cells; D011091:Polyesters / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011744:Pyrimidinones / Q000737:chemistry*; D051381:Rats; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D011091:Polyesters; D011744:Pyrimidinones; C016240:polycaprolactone; D011092:Polyethylene Glycols; C000595209:polyethylene glycol 1000
keywords: biomaterials; non-cell adhesive behavior; supramolecular polymers; vascular grafts