title: Secretome analysis of the mycoparasitic fungus Trichoderma harzianum ALL 42 cultivated in different media supplemented with Fusarium solani cell wall or glucose.
abstract: Trichoderma harzianum is a fungus well known for its potential as a biocontrol agent against many fungal phytopathogens. The aim of this study was to characterize the proteins secreted by T. harzianum ALL42 when its spores were inoculated and incubated for 48 h in culture media supplemented with glucose (GLU) or with cell walls from Fusarium solani (FSCW), a phytopathogen that causes severe losses in common bean and soy crops in Brazil, as well as other crop diseases around the world. Trichoderma harzianum was able to grow in Trichoderma Liquid Enzyme Production medium (TLE) and Minimal medium (MM) supplemented with FSCW and in TLE+GLU, but was unable to grow in MM+GLU medium. Protein quantification showed that TLE+FSCW and MM+FSCW had 45- and 30- fold, respectively, higher protein concentration on supernatant when compared to TLE+GLU, and this difference was observable on 2D gel electrophoresis (2DE). A total of 94 out of 105 proteins excised from 2DE maps were identified. The only protein observed in all three conditions was epl1. In the media supplemented with FSCW, different hydrolases such as chitinases, β-1,3-glucanases, glucoamylases, α-1,3-glucanases and proteases were identified, along with other proteins with no known functions in mycoparasitism, such as npp1 and cys. Trichoderma harzianum showed a complex and diverse arsenal of proteins that are secreted in response to the presence of FSCW, with novel proteins not previously described in mycoparasitic-related studies.
authors.affiliation: Departamento de Biologia Celular, Universidade de Brasília, Campus Universitário Darcy Ribeiro, Instituto de Ciências Biológicas, Brasília, DF, Brazil.|Laboratório de Espectrometria de Massa, Embrapa-Recursos Genéticos e Biotecnologia, Parque Estação Biológica, Brasília, DF, Brazil.
authors.forename: Marcelo Henrique Soller
authors.initials: MH
authors.lastname: Ramada
authors.affiliation: Departamento de Biologia Celular, Universidade de Brasília, Campus Universitário Darcy Ribeiro, Instituto de Ciências Biológicas, Brasília, DF, Brazil.
authors.forename: Andrei Stecca
authors.initials: AS
authors.lastname: Steindorff
authors.affiliation: Laboratório de Espectrometria de Massa, Embrapa-Recursos Genéticos e Biotecnologia, Parque Estação Biológica, Brasília, DF, Brazil.
authors.forename: Carlos
authors.initials: C
authors.lastname: Bloch
authors.affiliation: Laboratório de Enzimologia, Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Goiás (ICB), Goiânia, GO, Brazil.
authors.forename: Cirano José
authors.initials: CJ
authors.lastname: Ulhoa
mesh_terms: D000898:Antibiosis; D061046:Biological Control Agents; D002473:Cell Wall / Q000737:chemistry* / Q000378:metabolism; D002688:Chitinases / Q000235:genetics / Q000378:metabolism; D045424:Complex Mixtures / Q000378:metabolism / Q000494:pharmacology; D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology; D015180:Electrophoresis, Gel, Two-Dimensional; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D005670:Fusarium / Q000737:chemistry* / Q000472:pathogenicity; D015870:Gene Expression; D043326:Glucan 1,3-beta-Glucosidase / Q000235:genetics / Q000378:metabolism; D005087:Glucan 1,4-alpha-Glucosidase / Q000235:genetics / Q000378:metabolism; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology*; D051548:Histone Acetyltransferases / Q000235:genetics / Q000378:metabolism; D058977:Molecular Sequence Annotation; D010727:Phosphoric Diester Hydrolases / Q000235:genetics / Q000378:metabolism; D010935:Plant Diseases / Q000382:microbiology; D013025:Glycine max / Q000382:microbiology; D014242:Trichoderma / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*
chemical_list: D061046:Biological Control Agents; D045424:Complex Mixtures; D003470:Culture Media; D005656:Fungal Proteins; D051548:Histone Acetyltransferases; D010727:Phosphoric Diester Hydrolases; D002688:Chitinases; D005087:Glucan 1,4-alpha-Glucosidase; D043326:Glucan 1,3-beta-Glucosidase; D005947:Glucose
keywords: De novo peptide sequencing; Fusarium solani; Gene analysis; Microbiology; Secretome; Trichoderma harzianum