title: Wrap-and-Strip Technology of Protein-Polyelectrolyte Complex for Biomedical Application.
abstract: A polyelectrolyte is a polymer composed of repeating units of an electrolyte group that enables reversible complex formation with proteins in aqueous solutions. This review introduces "wrap-and-strip" technology of protein-polyelectrolyte complex (PPC) by noncovalent interaction. Storage: protein is stabilized against physical and chemical stresses. Enrichment: precipitation through PPC can be used as an enrichment method without irreversible unfolding. Catalytic activity switch: a complementary charged pair of polyelectrolytes functions as a reversible enzyme activity switch. Hyperactivation: a specific combination of a polyelectrolyte and substrate enhances enzyme activity by one order of magnitude compared with an enzyme alone. Stabilization: PPC increases protein stability against chemical and physical stresses, such as covalently modified polyethylene glycosylated protein. Simple PPC-based technology can expand the applicable fields of soluble proteins in aqueous solutions.
authors.affiliation: Faculty of Pure and Applied Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8573, Japan. shiraki@bk.tsukuba.ac.jp.
authors.forename: Kentaro
authors.initials: K
authors.lastname: Shiraki
authors.forename: Takaaki
authors.initials: T
authors.lastname: Kurinomaru
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Tomita
mesh_terms: D000906:Antibodies / Q000737:chemistry / Q000378:metabolism; D055162:Biocatalysis; D002626:Chemistry, Pharmaceutical; D004573:Electrolytes / Q000737:chemistry*; D004798:Enzymes / Q000378:metabolism; D006801:Humans; D011108:Polymers / Q000737:chemistry; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism
chemical_list: D000906:Antibodies; D004573:Electrolytes; D004798:Enzymes; D011108:Polymers; D011506:Proteins