title: Glycometabolic reprogramming associated with the initiation of human dental pulp stem cell differentiation.
abstract: Glycometabolism, particularly mitochondrial oxidative phosphorylation (OXPHOS) and glycolysis, plays a central role in cell life activities. Glycometabolism can be reprogrammed to maintain the stemness or to induce the differentiation of stem cells, thereby regulating tissue repair and regeneration. However, research on the glycometabolism of human dental pulp stem cells (hDPSCs) remains scarce. Here, we investigated the relationship between glycometabolic reprogramming and initiation of hDPSC differentiation. We found the differentiation of hDPSCs commenced on day 3 when cells were cultured in mineralized medium. When cell differentiation commenced, mitochondria became elongated with well-developed cristae, and the oxygen consumption rate of mitochondria was enhanced, manifested as an increase in basal respiration, mitochondrial ATP production, and maximal respiration. Interestingly, glycolytic enzyme activities, glycolysis capacity, and glycolysis reserve were also upregulated at this time to match the powerful bioenergetic demands. More importantly, hDPSCs derived from different donors or cultured in various oxygen environments showed similar glycometabolic changes when they began to differentiate. Thus, glycometabolic reprogramming accompanies initiation of hDPSC differentiation and could potentially play a role in the regulation of dental pulp repair.
authors.affiliation: State Key Laboratory of Oral Diseases, Department of Operative Dentistry and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
authors.forename: Linyan
authors.initials: L
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Oral Diseases, Department of Preventive Dentistry, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
authors.forename: Li
authors.initials: L
authors.lastname: Cheng
authors.affiliation: State Key Laboratory of Oral Diseases, Department of Operative Dentistry and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
authors.forename: Huning
authors.initials: H
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Oral Diseases, Department of Operative Dentistry and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
authors.forename: Hongying
authors.initials: H
authors.lastname: Pan
authors.affiliation: State Key Laboratory of Oral Diseases, Department of Operative Dentistry and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Yang
authors.affiliation: College of Life Sciences, National Engineering Laboratory for Oral Regenerative Medicine Sichuan, University, Chengdu, Sichuan, 610041, China.
authors.forename: Meiying
authors.initials: M
authors.lastname: Shao
authors.affiliation: State Key Laboratory of Oral Diseases, Department of Operative Dentistry and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, China.|State Key Laboratory of Oral Diseases, Department of Preventive Dentistry, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Hu
mesh_terms: D000293:Adolescent; D002454:Cell Differentiation / Q000502:physiology*; D015687:Cell Hypoxia; D002478:Cells, Cultured; D065150:Cellular Reprogramming; D003782:Dental Pulp / Q000166:cytology*; D006019:Glycolysis; D006593:Hexokinase / Q000378:metabolism; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism; D010085:Oxidative Phosphorylation; D011770:Pyruvate Kinase / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*; D055815:Young Adult
chemical_list: D007770:L-Lactate Dehydrogenase; D006593:Hexokinase; D011770:Pyruvate Kinase
keywords: dental pulp stem cells; differentiation; glycolysis; glycometabolic reprogramming; hypoxia; mitochondrial oxidative phosphorylation