title: Recovery and purification of chitosanase produced by Bacillus cereus using expanded bed adsorption and central composite design.
abstract: This study presents a system for expanded bed adsorption for the purification of chitosanase from broth extract in a single step. A chitosanase-producing strain was isolated and identified as Bacillus cereus C-01 and used to produce chitosanases. The expanded bed adsorption conditions for chitosanase purification were optimized statistically using STREAMLINE(TM) DEAE and a homemade column (2.6 × 30.0 cm). Dependent variables were defined by the quality criteria purification factor (P) and enzyme yield to optimize the chromatographic process. Statistical analyses showed that the optimum conditions for the maximum P were 150 cm/h load flow velocity, 6.0 cm settled bed height, and 7.36 cm distributor height. Distributor height had a strong influence on the process, considerably affecting both the P and enzyme yield. Optimizing the purification variables resulted in an approximately 3.66-fold increase in the P compared with the value under nonoptimized conditions. This system is promising for the recovery of chitosanase from B. cereus C-01 and is economically viable because it promotes the reduction steps.
authors.affiliation: Department of Chemical Engineering, Technology Center, Universidade Federal do Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil.
authors.forename: Nathália Kelly
authors.initials: NK
authors.lastname: de Araújo
authors.affiliation: Department of Chemical Engineering, Technology Center, Universidade Federal do Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil.
authors.forename: Vanessa Carvalho
authors.initials: VC
authors.lastname: Pimentel
authors.affiliation: Department of Chemical Engineering, Technology Center, Universidade Federal do Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil.
authors.forename: Nayane Macedo Portela
authors.initials: NM
authors.lastname: da Silva
authors.affiliation: Department of Chemical Engineering, Technology Center, Universidade Federal do Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil.
authors.forename: Carlos Eduardo
authors.initials: CE
authors.lastname: de Araújo Padilha
authors.affiliation: Department of Chemical Engineering, Technology Center, Universidade Federal do Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil.
authors.forename: Gorete Ribeiro
authors.initials: GR
authors.lastname: de Macedo
authors.affiliation: Department of Chemical Engineering, Technology Center, Universidade Federal do Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil.
authors.forename: Everaldo Silvino
authors.initials: ES
authors.lastname: Dos Santos
mesh_terms: D000327:Adsorption; D001409:Bacillus cereus / Q000201:enzymology*; D002021:Buffers; D048271:Chitosan / Q000737:chemistry; D002845:Chromatography / Q000379:methods; D004983:Ethanolamines; D006026:Glycoside Hydrolases / Q000737:chemistry / Q000302:isolation & purification*; D057446:Hydrodynamics; D006863:Hydrogen-Ion Concentration; D007218:Industrial Microbiology / Q000379:methods; D008024:Ligands; D008970:Molecular Weight; D012044:Regression Analysis
chemical_list: D002021:Buffers; D004983:Ethanolamines; D008024:Ligands; D048271:Chitosan; D006026:Glycoside Hydrolases; C019868:chitosanase; C007369:2-diethylaminoethanol
keywords: Bioseparation; Chitosanase; Downstream Processing; Expanded bed adsorption