title: Specific immunotherapy in combination with Clostridium butyricum inhibits allergic inflammation in the mouse intestine.
abstract: The current therapy on allergic inflammation is unsatisfactory. Probiotics improve the immunity in the body. This study aims to test a hypothesis that administration with Clostridium butyricum (C. butyricum) enforces the effect of specific immunotherapy (SIT) on intestinal allergic inflammation. In this study, an ovalbumin (OVA) specific allergic inflammation mouse model was created. The mice were treated with SIT or/and C. butyricum. The results showed that the intestinal allergic inflammation was only moderately alleviated by SIT, which was significantly enforced by a combination with C. butyricum; treating with C. butyricum alone did not show much inhibitory efficacy. The increase in the frequency of the interleukin (IL)-10-producing OVA-specific B cell (OVAsBC) was observed in mice in parallel to the inhibitory effect on the intestinal allergic inflammation. The in vitro treatment of the OVAsBCs with OVA increased the histone deacetylase-1 (HDAC1) phosphorylation, modulated the transcription of the Bcl6 gene, and triggered the OVAsBCs to differentiate to the IgE-producing plasma cells. Exposure to both OVA and butyrate sodium in the culture increased the expression of IL-10 in OVAsBCs. In conclusion, administration with C. butyricum enforces the inhibitory effect of SIT on allergic inflammation in the mouse intestine.
authors.affiliation: Department of Gastroenterology, the Shanghai Tenth People's Hospital of Tongji University, Shanghai 200072, China.
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Shi
authors.affiliation: ENT Institute of Shenzhen University School of Medicine, Shenzhen Key Laboratory of Allergy &Immunology, Shenzhen, 518060, China.
authors.forename: Ling-Zhi
authors.initials: LZ
authors.lastname: Xu
authors.affiliation: Department of Gastroenterology, the Shanghai Tenth People's Hospital of Tongji University, Shanghai 200072, China.
authors.forename: Kangsheng
authors.initials: K
authors.lastname: Peng
authors.affiliation: Department of Gastroenterology, the Shanghai Tenth People's Hospital of Tongji University, Shanghai 200072, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wu
authors.affiliation: Department of Gastroenterology, the Shanghai Tenth People's Hospital of Tongji University, Shanghai 200072, China.
authors.forename: Ruijin
authors.initials: R
authors.lastname: Wu
authors.affiliation: ENT Institute of Shenzhen University School of Medicine, Shenzhen Key Laboratory of Allergy &Immunology, Shenzhen, 518060, China.|Longgang Central Hospital, Shenzhen 518116, China.
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Liu
authors.affiliation: ENT Institute of Shenzhen University School of Medicine, Shenzhen Key Laboratory of Allergy &Immunology, Shenzhen, 518060, China.|Longgang Central Hospital, Shenzhen 518116, China.
authors.forename: Gui
authors.initials: G
authors.lastname: Yang
authors.affiliation: ENT Institute of Shenzhen University School of Medicine, Shenzhen Key Laboratory of Allergy &Immunology, Shenzhen, 518060, China.|Longgang Central Hospital, Shenzhen 518116, China.
authors.forename: Xiao-Rui
authors.initials: XR
authors.lastname: Geng
authors.affiliation: Shenzhen Women &Children Healthcare Center, Shenzhen, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.affiliation: ENT Institute of Shenzhen University School of Medicine, Shenzhen Key Laboratory of Allergy &Immunology, Shenzhen, 518060, China.
authors.forename: Zhi-Gang
authors.initials: ZG
authors.lastname: Liu
authors.affiliation: Department of Gastroenterology, the Shanghai Tenth People's Hospital of Tongji University, Shanghai 200072, China.
authors.forename: Zhanju
authors.initials: Z
authors.lastname: Liu
authors.affiliation: ENT Institute of Shenzhen University School of Medicine, Shenzhen Key Laboratory of Allergy &Immunology, Shenzhen, 518060, China.
authors.forename: Ping-Chang
authors.initials: PC
authors.lastname: Yang
mesh_terms: D000818:Animals; D046972:Clostridium butyricum; D056284:Histone Deacetylase 1 / Q000235:genetics / Q000276:immunology; D006967:Hypersensitivity / Q000235:genetics / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D007073:Immunoglobulin E / Q000235:genetics / Q000276:immunology; D007167:Immunotherapy; D007249:Inflammation / Q000139:chemically induced / Q000276:immunology / Q000473:pathology; D016753:Interleukin-10 / Q000235:genetics / Q000276:immunology; D007422:Intestines / Q000276:immunology* / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D008817:Mice, Mutant Strains; D010950:Plasma Cells / Q000276:immunology / Q000473:pathology; D019936:Probiotics / Q000493:pharmacokinetics*
chemical_list: C556062:IL10 protein, mouse; D016753:Interleukin-10; D007073:Immunoglobulin E; C484389:Hdac1 protein, mouse; D056284:Histone Deacetylase 1