title: Multimodal targeted high relaxivity thermosensitive liposome for in vivo imaging.
abstract: Liposomes are spherical, self-closed structures formed by lipid bilayers that can encapsulate drugs and/or imaging agents in their hydrophilic core or within their membrane moiety, making them suitable delivery vehicles. We have synthesized a new liposome containing gadolinium-DOTA lipid bilayer, as a targeting multimodal molecular imaging agent for magnetic resonance and optical imaging. We showed that this liposome has a much higher molar relaxivities r1 and r2 compared to a more conventional liposome containing gadolinium-DTPA-BSA lipid. By incorporating both gadolinium and rhodamine in the lipid bilayer as well as biotin on its surface, we used this agent for multimodal imaging and targeting of tumors through the strong biotin-streptavidin interaction. Since this new liposome is thermosensitive, it can be used for ultrasound-mediated drug delivery at specific sites, such as tumors, and can be guided by magnetic resonance imaging.
authors.affiliation: Experimental Therapeutics and Molecular Imaging Laboratory, Neuroscience Center, Massachusetts General Hospital, Boston, MA 02114 USA.|Program in Neuroscience, Harvard Medical School, Boston, MA 02114 USA.|Department of Biomedical Engineering, Biomedical NMR, Eindhoven University of Technology, Eindhoven, the Netherlands.
authors.forename: Maayke M P
authors.initials: MM
authors.lastname: Kuijten
authors.affiliation: Experimental Therapeutics and Molecular Imaging Laboratory, Neuroscience Center, Massachusetts General Hospital, Boston, MA 02114 USA.|Program in Neuroscience, Harvard Medical School, Boston, MA 02114 USA.|Department of Neurosurgery, Leiden University Medical Center, Leiden, the Netherlands.
authors.forename: M
authors.initials: M
authors.lastname: Hannah Degeling
authors.affiliation: Division of Neuroradiology, Department of Radiology, Massachusetts General Hospital, Boston, MA 02114 USA.|Center for Systems Biology, Massachusetts General Hospital, Boston, MA 02114 USA.
authors.forename: John W
authors.initials: JW
authors.lastname: Chen
authors.affiliation: Center for Systems Biology, Massachusetts General Hospital, Boston, MA 02114 USA.
authors.forename: Gregory
authors.initials: G
authors.lastname: Wojtkiewicz
authors.affiliation: Center for Systems Biology, Massachusetts General Hospital, Boston, MA 02114 USA.
authors.forename: Peter
authors.initials: P
authors.lastname: Waterman
authors.affiliation: Center for Systems Biology, Massachusetts General Hospital, Boston, MA 02114 USA.
authors.forename: Ralph
authors.initials: R
authors.lastname: Weissleder
authors.affiliation: Transplantation Research Center, Renal Division, Brigham and Women's Hospital and Children's Hospital, Harvard Medical School, Boston, Boston, MA 02114 USA.
authors.forename: Jamil
authors.initials: J
authors.lastname: Azzi
authors.affiliation: Department of Biomedical Engineering, Biomedical NMR, Eindhoven University of Technology, Eindhoven, the Netherlands.
authors.forename: Klaas
authors.initials: K
authors.lastname: Nicolay
authors.affiliation: Experimental Therapeutics and Molecular Imaging Laboratory, Neuroscience Center, Massachusetts General Hospital, Boston, MA 02114 USA.|Program in Neuroscience, Harvard Medical School, Boston, MA 02114 USA.
authors.forename: Bakhos A
authors.initials: BA
authors.lastname: Tannous
mesh_terms: D000818:Animals; D001710:Biotin / Q000737:chemistry / Q000378:metabolism; D001932:Brain Neoplasms / Q000175:diagnosis / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D003287:Contrast Media / Q000737:chemistry / Q000493:pharmacokinetics; D066246:ErbB Receptors / Q000235:genetics / Q000378:metabolism; D019786:Gadolinium DTPA / Q000737:chemistry / Q000493:pharmacokinetics; D015870:Gene Expression; D005910:Glioma / Q000175:diagnosis / Q000378:metabolism / Q000473:pathology*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006571:Heterocyclic Compounds / Q000737:chemistry / Q000493:pharmacokinetics*; D006801:Humans; D007279:Injections, Subcutaneous; D008051:Lipid Bilayers / Q000737:chemistry / Q000493:pharmacokinetics; D008081:Liposomes / Q000737:chemistry / Q000493:pharmacokinetics*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D061848:Optical Imaging / Q000379:methods*; D009942:Organometallic Compounds / Q000737:chemistry / Q000493:pharmacokinetics*; D011485:Protein Binding; D012235:Rhodamines / Q000737:chemistry; D019809:Streptavidin / Q000737:chemistry / Q000378:metabolism; D013696:Temperature
chemical_list: D003287:Contrast Media; D006571:Heterocyclic Compounds; D008051:Lipid Bilayers; D008081:Liposomes; D009942:Organometallic Compounds; D012235:Rhodamines; C410723:epidermal growth factor receptor VIII; D049452:Green Fluorescent Proteins; D001710:Biotin; D019809:Streptavidin; C050823:gadolinium 1,4,7,10-tetraazacyclododecane-N,N',N'',N'''-tetraacetate; D066246:ErbB Receptors; D019786:Gadolinium DTPA