title: Immunomodulation properties of multi-species fermented milks.
abstract: Dairy propionibacteria (PAB) are used as a ripening starter in combination with Lactic acid bacteria (LAB) for dairy products such as Swiss-type cheese. LAB and PAB have also been studied for their probiotic properties but little is still known about their individual and/or synergistic beneficial effects within dairy matrices. In the context of a rising incidence of Inflammatory Bowel Diseases, it has become crucial to evaluate the immunomodulatory potential of bacteria ingested in large numbers via dairy products. We therefore selected different strains and combinations of technological LAB and PAB. We determined their immunomodulatory potential by IL-10 and IL-12 induction, in human peripheral blood mononuclear cells, on either single or mixed cultures, grown on laboratory medium or directly in milk. Milk was fermented with selected anti-inflammatory strains of LAB or PAB/LAB mixed cultures and the resulting bacterial fractions were also evaluated for these properties, together with starter viability and optimum technological aspects. The most promising fermented milks were evaluated in the context of TNBS- or DSS-induced colitis in mice. The improvement in inflammatory parameters evidenced an alleviation of colitis symptoms as a result of fermented milk consumption. This effect was clearly strain-dependent and modulated by growth within a fermented dairy product. These findings offer new tools and perspectives for the development of immunomodulatory fermented dairy products for targeted populations.
authors.affiliation: Lactic Acid Bacteria & Mucosal Immunity, Center for Infection and Immunity of Lille, Institut Pasteur de Lille, INSERM-U 1019, CNRS UMR 8204 Université de Lille, 1 rue du Pr Calmette, BP 245, F-59019 Lille, France.
authors.forename: Benoît
authors.initials: B
authors.lastname: Foligné
authors.affiliation: INRA, UMR 1253 Science et Technologie du Lait et de l'Œuf, F-35042 Rennes, France; AGROCAMPUS OUEST, UMR1253 UMR Science et Technologie du Lait et de l' Œuf, F-35042 Rennes, France.
authors.forename: Sandrine
authors.initials: S
authors.lastname: Parayre
authors.affiliation: INRA, UMR 1253 Science et Technologie du Lait et de l'Œuf, F-35042 Rennes, France; AGROCAMPUS OUEST, UMR1253 UMR Science et Technologie du Lait et de l' Œuf, F-35042 Rennes, France.
authors.forename: Redouane
authors.initials: R
authors.lastname: Cheddani
authors.affiliation: INRA, UMR 1253 Science et Technologie du Lait et de l'Œuf, F-35042 Rennes, France; AGROCAMPUS OUEST, UMR1253 UMR Science et Technologie du Lait et de l' Œuf, F-35042 Rennes, France.
authors.forename: Marie-Hélène
authors.initials: MH
authors.lastname: Famelart
authors.affiliation: INRA, UMR 1253 Science et Technologie du Lait et de l'Œuf, F-35042 Rennes, France; AGROCAMPUS OUEST, UMR1253 UMR Science et Technologie du Lait et de l' Œuf, F-35042 Rennes, France.
authors.forename: Marie-Noëlle
authors.initials: MN
authors.lastname: Madec
authors.affiliation: Lactic Acid Bacteria & Mucosal Immunity, Center for Infection and Immunity of Lille, Institut Pasteur de Lille, INSERM-U 1019, CNRS UMR 8204 Université de Lille, 1 rue du Pr Calmette, BP 245, F-59019 Lille, France.
authors.forename: Coline
authors.initials: C
authors.lastname: Plé
authors.affiliation: Lactic Acid Bacteria & Mucosal Immunity, Center for Infection and Immunity of Lille, Institut Pasteur de Lille, INSERM-U 1019, CNRS UMR 8204 Université de Lille, 1 rue du Pr Calmette, BP 245, F-59019 Lille, France.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Breton
authors.affiliation: Lactic Acid Bacteria & Mucosal Immunity, Center for Infection and Immunity of Lille, Institut Pasteur de Lille, INSERM-U 1019, CNRS UMR 8204 Université de Lille, 1 rue du Pr Calmette, BP 245, F-59019 Lille, France.
authors.forename: Joëlle
authors.initials: J
authors.lastname: Dewulf
authors.affiliation: INRA, UMR 1253 Science et Technologie du Lait et de l'Œuf, F-35042 Rennes, France; AGROCAMPUS OUEST, UMR1253 UMR Science et Technologie du Lait et de l' Œuf, F-35042 Rennes, France.
authors.forename: Gwénaël
authors.initials: G
authors.lastname: Jan
authors.affiliation: INRA, UMR 1253 Science et Technologie du Lait et de l'Œuf, F-35042 Rennes, France; AGROCAMPUS OUEST, UMR1253 UMR Science et Technologie du Lait et de l' Œuf, F-35042 Rennes, France.
authors.forename: Stéphanie-Marie
authors.initials: SM
authors.lastname: Deutsch
mesh_terms: D000818:Animals; D043302:Cultured Milk Products / Q000276:immunology* / Q000382:microbiology*; D006801:Humans; D056747:Immunomodulation; D015212:Inflammatory Bowel Diseases / Q000628:therapy; D016753:Interleukin-10 / Q000096:biosynthesis / Q000276:immunology; D018664:Interleukin-12 / Q000096:biosynthesis / Q000276:immunology; D007777:Lactobacillaceae / Q000276:immunology / Q000502:physiology*; D007963:Leukocytes, Mononuclear / Q000276:immunology; D051379:Mice; D019936:Probiotics / Q000378:metabolism; D011424:Propionibacterium / Q000276:immunology / Q000502:physiology*
chemical_list: C508609:IL10 protein, human; D016753:Interleukin-10; D018664:Interleukin-12
keywords: Anti-inflammatory; Dairy; Immunomodulation; Lactic acid bacteria; Propionibacteria; Propionibacterium freudenreichii