title: Elastic staining does not assist detection of venous invasion in cutaneous melanoma.
abstract: The aim of the present study was to determine the benefit of orcein elastic staining of primary cutaneous melanoma specimens in detecting venous invasion. Primary cutaneous melanomas in vertical growth phase were assessed for vascular invasion. All tumour blocks were stained with haematoxylin and eosin (H&E) and orcein. The cases were reviewed by two pathologists. Vascular invasion was not identified more frequently on orcein stained slides than on H&E stained ones. Elastosis and periappendiceal elastic fibres interfered with vascular invasion detection with elastic staining. Based on our study, we conclude that elastic stains such as orcein do not improve the detection rate of venous invasion in primary cutaneous melanomas.
authors.affiliation: Department of Pathology, Bács-Kiskun County Teaching Hospital, Nyíri út 38, H-6000 Kecskemét, Hungary. Electronic address: sejbenist@freemail.hu.
authors.forename: István
authors.initials: I
authors.lastname: Sejben
authors.affiliation: Department of Pathology, Bács-Kiskun County Teaching Hospital, Nyíri út 38, H-6000 Kecskemét, Hungary; Department of Dermatology, Bács-Kiskun County Teaching Hospital, Nyíri út 38, H-6000 Kecskemét, Hungary.
authors.forename: Lajos
authors.initials: L
authors.lastname: Kocsis
authors.affiliation: Department of Dermatology, Bács-Kiskun County Teaching Hospital, Nyíri út 38, H-6000 Kecskemét, Hungary.
authors.forename: László
authors.initials: L
authors.lastname: Török
authors.affiliation: Department of Pathology, Bács-Kiskun County Teaching Hospital, Nyíri út 38, H-6000 Kecskemét, Hungary; Department of Pathology, University of Szeged, Állomás u. 2, H-6720 Szeged, Hungary.
authors.forename: Gábor
authors.initials: G
authors.lastname: Cserni
mesh_terms: D000230:Adenocarcinoma; D015179:Colorectal Neoplasms / Q000473:pathology; D004547:Elastic Tissue / Q000473:pathology*; D006801:Humans; D008545:Melanoma / Q000175:diagnosis*; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D011379:Prognosis; D012878:Skin Neoplasms / Q000473:pathology*; D013194:Staining and Labeling
keywords: Elastic stain; Melanoma; Orcein; Venous invasion