title: Expression studies of neuronatin in prenatal and postnatal rat pituitary.
abstract: The pituitary gland, an indispensable endocrine organ that synthesizes and secretes pituitary hormones, develops with the support of many factors. Among them, neuronatin (NNAT), which was discovered in the neonatal mouse brain as a factor involved in neural development, has subsequently been revealed to be coded by an abundantly expressing gene in the pituitary gland but its role remains elusive. We analyze the expression profile of Nnat and the localization of its product during rat pituitary development. The level of Nnat expression was high during the embryonic period but remarkably decreased after birth. Immunohistochemistry demonstrated that NNAT appeared in the SOX2-positive stem/progenitor cells in the developing pituitary primordium on rat embryonic day 11.5 (E11.5) and later in the majority of SOX2/PROP1 double-positive cells on E13.5. Thereafter, during pituitary embryonic development, Nnat expression was observed in some stem/progenitor cells, proliferating cells and terminally differentiating cells. In postnatal pituitaries, NNAT-positive cells decreased in number, with most coexpressing Sox2 or Pit1, suggesting a similar role for NNAT to that during the embryonic period. NNAT was widely localized in mitochondria, peroxisomes and lysosomes, in addition to the endoplasmic reticulum but not in the Golgi. The present study thus demonstrated the variability in expression of NNAT-positive cells in rat embryonic and postnatal pituitaries and the intracellular localization of NNAT. Further investigations to obtain functional evidence for NNAT are a prerequisite.
authors.affiliation: Division of Life Science, Graduate School of Agriculture, Meiji University, Kanagawa, 214-8571, Japan.
authors.forename: Naoko
authors.initials: N
authors.lastname: Kanno
authors.affiliation: Organization for the Strategic Coordination of Research and Intellectual Property, Meiji University, Kanagawa, 214-8571, Japan.|Institute of Reproduction and Endocrinology, Meiji University, Kanagawa, 214-8571, Japan.
authors.forename: Masashi
authors.initials: M
authors.lastname: Higuchi
authors.affiliation: Division of Life Science, Graduate School of Agriculture, Meiji University, Kanagawa, 214-8571, Japan.
authors.forename: Saishu
authors.initials: S
authors.lastname: Yoshida
authors.affiliation: Division of Life Science, Graduate School of Agriculture, Meiji University, Kanagawa, 214-8571, Japan.
authors.forename: Hideji
authors.initials: H
authors.lastname: Yako
authors.affiliation: Organization for the Strategic Coordination of Research and Intellectual Property, Meiji University, Kanagawa, 214-8571, Japan.|Institute of Reproduction and Endocrinology, Meiji University, Kanagawa, 214-8571, Japan.
authors.forename: Mo
authors.initials: M
authors.lastname: Chen
authors.affiliation: Division of Life Science, Graduate School of Agriculture, Meiji University, Kanagawa, 214-8571, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Ueharu
authors.affiliation: Division of Life Science, Graduate School of Agriculture, Meiji University, Kanagawa, 214-8571, Japan.
authors.forename: Naoto
authors.initials: N
authors.lastname: Nishimura
authors.affiliation: Organization for the Strategic Coordination of Research and Intellectual Property, Meiji University, Kanagawa, 214-8571, Japan.|Institute of Reproduction and Endocrinology, Meiji University, Kanagawa, 214-8571, Japan.
authors.forename: Takako
authors.initials: T
authors.lastname: Kato
authors.affiliation: Division of Life Science, Graduate School of Agriculture, Meiji University, Kanagawa, 214-8571, Japan. yukato@meiji.ac.jp.|Institute of Reproduction and Endocrinology, Meiji University, Kanagawa, 214-8571, Japan. yukato@meiji.ac.jp.
authors.forename: Yukio
authors.initials: Y
authors.lastname: Kato
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D004721:Endoplasmic Reticulum / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D018398:Homeodomain Proteins / Q000378:metabolism; D008247:Lysosomes / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins / Q000096:biosynthesis*; D008928:Mitochondria / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D020675:Peroxisomes / Q000378:metabolism; D010902:Pituitary Gland / Q000196:embryology* / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D055748:SOXB1 Transcription Factors / Q000378:metabolism; D013234:Stem Cells / Q000378:metabolism*
chemical_list: D018398:Homeodomain Proteins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; C102134:Prophet of Pit-1 protein; D055748:SOXB1 Transcription Factors; C527784:Sox2 protein, rat; C088089:Nnat protein, rat
keywords: Neuronatin; PROP1; Rat pituitary; SOX2; Stem/progenitor cells