title: Persistence of Multi-Drug Resistance Plasmids in Sterile Water under Very Low Concentrations of Tetracycline.
abstract: The persistence of the multi-drug resistance plasmids pAQU1 and IncFIB was examined in bacterial populations under very low selective pressure. We herein demonstrated that these plasmids stably remained not only in the original host, but also in a transconjugant, even after being in a non-culturable state. In seawater microcosms containing Photobacterium damselae 04Ya311 possessing pAQU1, no significant loss of pAQU1 was observed during a 30-d starvation period. The copy numbers of pAQU1 and IncFIB in E. coli were constant. The results of the present study suggest that these plasmids have the ability to remain among various bacteria under oligotrophic conditions with low antibiotic selection pressure.
authors.affiliation: Center for Marine Environmental Studies (CMES), Ehime University.
authors.forename: Thi Lan Thanh
authors.initials: TL
authors.lastname: Bien
authors.forename: Yuki
authors.initials: Y
authors.lastname: Sato-Takabe
authors.forename: Mitsuko
authors.initials: M
authors.lastname: Ogo
authors.forename: Masaru
authors.initials: M
authors.lastname: Usui
authors.forename: Satoru
authors.initials: S
authors.lastname: Suzuki
mesh_terms: D000900:Anti-Bacterial Agents / Q000378:metabolism; D003227:Conjugation, Genetic; D024901:Drug Resistance, Multiple, Bacterial; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D042822:Genomic Instability; D010776:Photobacterium / Q000187:drug effects / Q000235:genetics*; D010957:Plasmids / Q000032:analysis*; D012623:Seawater / Q000382:microbiology*; D012641:Selection, Genetic; D013752:Tetracycline / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D013752:Tetracycline