title: Genetic variation in transpiration efficiency and relationships between whole plant and leaf gas exchange measurements in Saccharum spp. and related germplasm.
abstract: Fifty-one genotypes of sugarcane (Saccharum spp.) or closely related germplasm were evaluated in a pot experiment to examine genetic variation in transpiration efficiency. Significant variation in whole plant transpiration efficiency was observed, with the difference between lowest and highest genotypes being about 40% of the mean. Leaf gas exchange measurements were made across a wide range of conditions. There was significant genetic variation in intrinsic transpiration efficiency at a leaf level as measured by leaf internal CO2 (Ci) levels. Significant genetic variation in Ci was also observed within subsets of data representing narrow ranges of stomatal conductance. Ci had a low broad sense heritability (Hb = 0.11) on the basis of single measurements made at particular dates, because of high error variation and genotype × date interaction, but broad sense heritability for mean Ci across all dates was high (Hb = 0.81) because of the large number of measurements taken at different dates. Ci levels among genotypes at mid-range levels of conductance had a strong genetic correlation (-0.92 ± 0.30) with whole plant transpiration efficiency but genetic correlations between Ci and whole plant transpiration efficiency were weaker or not significant at higher and lower levels of conductance. Reduced Ci levels at any given level of conductance may result in improved yields in water-limited environments without trade-offs in rates of water use and growth. Targeted selection and improvement of lowered Ci per unit conductance via breeding may provide longer-term benefits for water-limited environments but the challenge will be to identify a low-cost screening methodology.
authors.affiliation: CSIRO, Australian Tropical Science Innovation Precinct, Private Mail Bag PO, Aitkenvale, QLD 4814, Australia phillip.jackson@csiro.au.
authors.forename: Phillip
authors.initials: P
authors.lastname: Jackson
authors.affiliation: Sugar Research Australia Limited, PO Box 117, Ayr, QLD 4807, Australia.
authors.forename: Jaya
authors.initials: J
authors.lastname: Basnayake
authors.affiliation: Crop Science Consulting, 33 Tamarind Street, Kirwin, QLD 4817, Australia.
authors.forename: Geoff
authors.initials: G
authors.lastname: Inman-Bamber
authors.affiliation: Sugar Research Australia Limited, 50 Meiers Road, Indooroopilly, QLD 4068, Australia.
authors.forename: Prakash
authors.initials: P
authors.lastname: Lakshmanan
authors.affiliation: Sugar Research Australia Limited, PO Box 117, Ayr, QLD 4807, Australia.
authors.forename: Sijesh
authors.initials: S
authors.lastname: Natarajan
authors.affiliation: CSIRO, Australian Tropical Science Innovation Precinct, Private Mail Bag PO, Aitkenvale, QLD 4814, Australia.
authors.forename: Chris
authors.initials: C
authors.lastname: Stokes
mesh_terms: D018533:Biomass; D002245:Carbon Dioxide / Q000378:metabolism; D005740:Gases / Q000378:metabolism*; D014644:Genetic Variation; D006813:Humidity; D010641:Phenotype; D010788:Photosynthesis / Q000235:genetics; D018515:Plant Leaves / Q000235:genetics* / Q000469:parasitology*; D018526:Plant Transpiration / Q000235:genetics*; D019655:Quantitative Trait, Heritable; D031786:Saccharum / Q000235:genetics* / Q000502:physiology*; D012639:Seeds / Q000235:genetics; D013696:Temperature
chemical_list: D005740:Gases; D002245:Carbon Dioxide
keywords: conductance; germplasm; photosynthesis; sugarcane; transpiration efficiency; water use efficiency.