title: Chemical Composition of Golden Berry Leaves Against Hepato-renal Fibrosis.
abstract: The role of Physalis peruviana (golden berry) as functional food against hepato-renal fibrosis induced by carbon tetrachloride (CCl4) was evaluated. The chemical composition of leaves referred the presence of withanolides and flavonoids. Two compounds, ursolic acid and lupeol, were isolated and their structures were elucidated by different spectral analysis techniques. The biological evaluation was conducted on different animal groups; control rats, control orally treated with plant extract (500 mg/kg body weight twice a week for six consecutive weeks), CCl4 (0.5 ml/kg body weight diluted to 1:9 (v/v) in olive oil and injected intraperitoneally) group, CCl4 treated with plant extract and CCl4 treated with silymarin as a reference herbal drug. The evaluation was done through measuring oxidative stress markers; malondialdehyde (MDA), superoxide dismutase (SOD) and nitric oxide (NO). Liver function indices; aspartate and alanine aminotransferases (AST & ALT), alkaline phosphatase (ALP), gamma glutamyl transferase (GGT), bilirubin and total hepatic protein were also estimated. Kidney disorder biomarkers; creatinine, urea and serum protein were also evaluated. The results revealed plant safety and decrease in NO, MDA, IgG, ALP, tissue protein, bilirubin, creatinine and urea levels. Increase in SOD, AST, ALT, GGT and serum protein levels were observed. Improvement in liver and kidney histopathological architectures were also seen. In conclusion, Physalis peruviana recorded a significant protective role in liver and kidney against fibrosis. Further studies are needed to evaluate its isolated compounds and its use in pharmacological applications and clinical uses.
authors.affiliation: a Food Science and Technology Department, Faculty of Agriculture , Cairo University , Cairo , Egypt.
authors.forename: Abd El-Rahman M
authors.initials: Ael-R
authors.lastname: Khalaf-Allah
authors.affiliation: b Medicinal & Aromatic Plants Department , National Research Center , Cairo , Egypt.
authors.forename: Souad E
authors.initials: SE
authors.lastname: El-Gengaihi
authors.affiliation: c Therapeutic Chemistry Department , National Research Center , Cairo , Egypt.
authors.forename: Manal A
authors.initials: MA
authors.lastname: Hamed
authors.affiliation: c Therapeutic Chemistry Department , National Research Center , Cairo , Egypt.
authors.forename: Hanan G
authors.initials: HG
authors.lastname: Zahran
authors.affiliation: b Medicinal & Aromatic Plants Department , National Research Center , Cairo , Egypt.
authors.forename: Mona A
authors.initials: MA
authors.lastname: Mohammed
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D000975:Antioxidants / Q000032:analysis / Q000494:pharmacology; D001219:Aspartate Aminotransferases / Q000097:blood; D001663:Bilirubin / Q000097:blood; D015415:Biomarkers / Q000097:blood; D002251:Carbon Tetrachloride / Q000633:toxicity; D003404:Creatinine / Q000097:blood; D005355:Fibrosis / Q000139:chemically induced / Q000188:drug therapy*; D005419:Flavonoids / Q000032:analysis / Q000494:pharmacology; D055951:Functional Food / Q000032:analysis*; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D053978:Pentacyclic Triterpenes / Q000032:analysis / Q000494:pharmacology; D032321:Physalis / Q000737:chemistry*; D010936:Plant Extracts / Q000032:analysis / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism; D014315:Triterpenes / Q000032:analysis / Q000494:pharmacology; D054358:Withanolides / Q000032:analysis / Q000494:pharmacology; D005723:gamma-Glutamyltransferase / Q000097:blood; D000097245:Ursolic Acid; D000094042:Lupanes
chemical_list: D000410:Alanine Transaminase; D000469:Alkaline Phosphatase; D000975:Antioxidants; D001219:Aspartate Aminotransferases; D001663:Bilirubin; D015415:Biomarkers; D002251:Carbon Tetrachloride; D003404:Creatinine; D005419:Flavonoids; D008315:Malondialdehyde; D009569:Nitric Oxide; D053978:Pentacyclic Triterpenes; D010936:Plant Extracts; D013482:Superoxide Dismutase; D014315:Triterpenes; D054358:Withanolides; D005723:gamma-Glutamyltransferase; D000097245:Ursolic Acid; C010480:lupeol; D000094042:Lupanes
keywords: Physallis peruviana; hepatoprotective; oxidative stress; renal injury