title: Yersinia enterocolitica Affects Intestinal Barrier Function in the Colon.
abstract: Infection with Yersinia enterocolitica causes acute diarrhea in early childhood. A mouse infection model presents new findings on pathological mechanisms in the colon. Symptoms involve diarrhea with watery feces and weight loss that have their functional correlates in decreased transepithelial electrical resistance and increased fluorescein permeability. Y. enterocolitica was present within the murine mucosa of both ileum and colon. Here, the bacterial insult was of focal nature and led to changes in tight junction protein expression and architecture. These findings are in concordance with observations from former cell culture studies and suggest a leak flux mechanism of diarrhea.
authors.affiliation: Department of General, Visceral, and Vascular Surgery, Institute of Clinical Physiology, Campus Benjamin Franklin, Charité-University Medicine Berlin.
authors.forename: Nina A
authors.initials: NA
authors.lastname: Hering
authors.affiliation: Department of Gastroenterology, Institute of Clinical Physiology, Campus Benjamin Franklin, Charité-University Medicine Berlin.
authors.forename: Anja
authors.initials: A
authors.lastname: Fromm
authors.affiliation: Biofilmcenter of the German Heart Institute Berlin, Germany.
authors.forename: Judith
authors.initials: J
authors.lastname: Kikhney
authors.affiliation: Department of Gastroenterology, Institute of Clinical Physiology, Campus Benjamin Franklin, Charité-University Medicine Berlin.
authors.forename: In-Fah M
authors.initials: IF
authors.lastname: Lee
authors.affiliation: Biofilmcenter of the German Heart Institute Berlin, Germany.
authors.forename: Annette
authors.initials: A
authors.lastname: Moter
authors.affiliation: Department of Gastroenterology, Institute of Clinical Physiology, Campus Benjamin Franklin, Charité-University Medicine Berlin.
authors.forename: Jörg D
authors.initials: JD
authors.lastname: Schulzke
authors.affiliation: Department of Gastroenterology, Institute of Clinical Physiology, Campus Benjamin Franklin, Charité-University Medicine Berlin.
authors.forename: Roland
authors.initials: R
authors.lastname: Bücker
mesh_terms: D000818:Animals; D003106:Colon / Q000382:microbiology* / Q000473:pathology; D003967:Diarrhea / Q000382:microbiology; D017097:Electric Impedance; D005243:Feces / Q000382:microbiology; D005260:Female; D015964:Gene Expression Regulation, Bacterial; D007413:Intestinal Mucosa / Q000382:microbiology* / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D013047:Specific Pathogen-Free Organisms; D062725:Tight Junction Proteins / Q000235:genetics / Q000378:metabolism; D015009:Yersinia Infections / Q000382:microbiology* / Q000473:pathology; D015008:Yersinia enterocolitica
chemical_list: D062725:Tight Junction Proteins
keywords: claudin; diarrheal mechanism; epithelial barrier function; mouse model; tight junction; yersiniosis