title: Enhanced biodegradation of pyrene and indeno(1,2,3-cd)pyrene using bacteria immobilized in cinder beads in estuarine wetlands.
abstract: Two strains (Pseudomonas taiwanensis PYR1 and Acinetobacter baumannii INP1) were isolated from PAH-contaminated Liaohe estuarine wetland using enrichment. The cells of PYR1 and INP1 were immobilized in cinder beads for pyrene and indeno(1,2,3-cd)pyrene biodegradation in wetland. Biodegradation of pyrene and indeno(1,2,3-cd)pyrene in soils from wetland was carried out in pots using free cells as well as those immobilized in cinder beads to ascertain the role of bioaugmentation. Supported by the cinder beads, the immobilized cells degraded 70.7% and 80.9% of pyrene and indeno(1,2,3-cd)pyrene respectively after 30 days. While the free cells degraded only 58.2% and 55.3%. Additionally, microbial analysis with high-throughput sequencing revealed the changes of microbial communities in soil without and with cinder beads immobilized with strains. The result indicated that Gammaproteobacteria were dominant PAH-degrading groups during bioaugmentation. This effective approach can be used to treat other PAH-contaminated wetlands by immobilizing different species of bacteria in cinder beads.
authors.affiliation: College of Environmental Science and Engineering, Ocean University of China, Shandong, Qingdao 266100, China.
authors.forename: Ru-Ying
authors.initials: RY
authors.lastname: Huang
authors.affiliation: College of Environmental Science and Engineering, Ocean University of China, Shandong, Qingdao 266100, China; Key Laboratory of Marine Environment and Ecology, Ministry of Education, Shandong, Qingdao 266100, China. Electronic address: weijunas@ouc.edu.cn.
authors.forename: Wei-Jun
authors.initials: WJ
authors.lastname: Tian
authors.affiliation: College of Environmental Science and Engineering, Ocean University of China, Shandong, Qingdao 266100, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Liu
authors.affiliation: College of Environmental Science and Engineering, Ocean University of China, Shandong, Qingdao 266100, China.
authors.forename: Hui-Bo
authors.initials: HB
authors.lastname: Yu
authors.affiliation: College of Environmental Science and Engineering, Ocean University of China, Shandong, Qingdao 266100, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Jin
authors.affiliation: College of Environmental Science and Engineering, Ocean University of China, Shandong, Qingdao 266100, China; Key Laboratory of Marine Environment and Ecology, Ministry of Education, Shandong, Qingdao 266100, China.
authors.forename: Yang-Guo
authors.initials: YG
authors.lastname: Zhao
authors.affiliation: College of Environmental Science and Engineering, Ocean University of China, Shandong, Qingdao 266100, China.
authors.forename: Yu-Hang
authors.initials: YH
authors.lastname: Zhou
authors.affiliation: College of Environmental Science and Engineering, Ocean University of China, Shandong, Qingdao 266100, China.
authors.forename: Gong
authors.initials: G
authors.lastname: Feng
mesh_terms: D040981:Acinetobacter baumannii / Q000378:metabolism; D001673:Biodegradation, Environmental; D020563:Gammaproteobacteria / Q000145:classification / Q000378:metabolism; D011549:Pseudomonas / Q000378:metabolism; D011721:Pyrenes / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D012987:Soil; D012988:Soil Microbiology; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D053833:Wetlands
chemical_list: D011721:Pyrenes; D012987:Soil; D014874:Water Pollutants, Chemical; C030984:pyrene; C041508:indeno(1,2,3-cd)pyrene
keywords: Biodegradation; Cinder beads; Immobilized; Indeno(1,2,3-cd)pyrene; Pyrene