title: DNA microarray unravels rapid changes in transcriptome of MK-801 treated rat brain.
abstract: OBJECTIVE
  To investigate the impact of MK-801 on gene expression patterns genome wide in rat brain regions.
  
  
  METHODS
  Rats were treated with an intraperitoneal injection of MK-801 [0.08 (low-dose) and 0.16 (high-dose) mg/kg] or NaCl (vehicle control). In a first series of experiment, the frontoparietal electrocorticogram was recorded 15 min before and 60 min after injection. In a second series of experiments, the whole brain of each animal was rapidly removed at 40 min post-injection, and different regions were separated: amygdala, cerebral cortex, hippocampus, hypothalamus, midbrain and ventral striatum on ice followed by DNA microarray (4 × 44 K whole rat genome chip) analysis.
  
  
  RESULTS
  Spectral analysis revealed that a single systemic injection of MK-801 significantly and selectively augmented the power of baseline gamma frequency (30-80 Hz) oscillations in the frontoparietal electroencephalogram. DNA microarray analysis showed the largest number (up- and down- regulations) of gene expressions in the cerebral cortex (378), midbrain (376), hippocampus (375), ventral striatum (353), amygdala (301), and hypothalamus (201) under low-dose (0.08 mg/kg) of MK-801. Under high-dose (0.16 mg/kg), ventral striatum (811) showed the largest number of gene expression changes. Gene expression changes were functionally categorized to reveal expression of genes and function varies with each brain region.
  
  
  CONCLUSIONS
  Acute MK-801 treatment increases synchrony of baseline gamma oscillations, and causes very early changes in gene expressions in six individual rat brain regions, a first report.
authors.affiliation: Yuka Kobayashi, Hiroyuki Satoh, Department of Biomolecular Science, Faculty of Science, Toho University, Chiba 274-8510, Japan.
authors.forename: Yuka
authors.initials: Y
authors.lastname: Kobayashi
authors.affiliation: Yuka Kobayashi, Hiroyuki Satoh, Department of Biomolecular Science, Faculty of Science, Toho University, Chiba 274-8510, Japan.
authors.forename: Sofya P
authors.initials: SP
authors.lastname: Kulikova
authors.affiliation: Yuka Kobayashi, Hiroyuki Satoh, Department of Biomolecular Science, Faculty of Science, Toho University, Chiba 274-8510, Japan.
authors.forename: Junko
authors.initials: J
authors.lastname: Shibato
authors.affiliation: Yuka Kobayashi, Hiroyuki Satoh, Department of Biomolecular Science, Faculty of Science, Toho University, Chiba 274-8510, Japan.
authors.forename: Randeep
authors.initials: R
authors.lastname: Rakwal
authors.affiliation: Yuka Kobayashi, Hiroyuki Satoh, Department of Biomolecular Science, Faculty of Science, Toho University, Chiba 274-8510, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Satoh
authors.affiliation: Yuka Kobayashi, Hiroyuki Satoh, Department of Biomolecular Science, Faculty of Science, Toho University, Chiba 274-8510, Japan.
authors.forename: Didier
authors.initials: D
authors.lastname: Pinault
authors.affiliation: Yuka Kobayashi, Hiroyuki Satoh, Department of Biomolecular Science, Faculty of Science, Toho University, Chiba 274-8510, Japan.
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Masuo
keywords: Dizocilpine; Dye-swap; Gene expression; MK801; Microarray; N-Methyl-D-aspartate receptors