title: Biomechanical profile of cancer stem-like cells derived from MHCC97H cell lines.
abstract: Biomechanical properties and cytoskeletal organization of cancer cells are known to be closely related with their aggressive phenotype. In this study, based on atomic force microscopy (AFM), we aimed to evaluate the mechanical property of liver cancer stem-like cells (LCSCs) and compare it with human hepatoma cells (HHCs). LCSCs were enriched from human hepatoma cell line MHCC97H through a sphere culture system. AFM nanoindentation was investigated as a method for measuring the cell stiffness, and reflecting by Young׳s modulus. Microfilament bundles of F-actin were observed with immunofluorescence staining by confocal microscopy. We found that LCSCs show lower Young׳s modulus and higher migration ability compared to MHCC97H cells. Moreover, the decrease in Young׳s modulus is accompanied with a dramatic decline in F-actin content. These results demonstrated a close relationship between the cell Young׳s modulus and metastatic potential of HHCs, which suggest that Young׳s modulus detected by AFM can be used to evaluate metastatic potential of cancer cells.
authors.affiliation: Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400044, People׳s Republic of China. Electronic address: sunjhemail@163.com.
authors.forename: Jinghui
authors.initials: J
authors.lastname: Sun
authors.affiliation: Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400044, People׳s Republic of China. Electronic address: qing.luo@cqu.edu.cn.
authors.forename: Qing
authors.initials: Q
authors.lastname: Luo
authors.affiliation: Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400044, People׳s Republic of China. Electronic address: liulingling2012@163.com.
authors.forename: Lingling
authors.initials: L
authors.lastname: Liu
authors.affiliation: Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400044, People׳s Republic of China. Electronic address: zhangbingyu1111@126.com.
authors.forename: Bingyu
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400044, People׳s Republic of China. Electronic address: shiyis@cqu.edu.cn.
authors.forename: Yisong
authors.initials: Y
authors.lastname: Shi
authors.affiliation: Department of Mechanical Science and Engineering, Nagoya University, Nagoya 464-8603, Japan. Electronic address: ju@mech.nagoya-u.ac.jp.
authors.forename: Yang
authors.initials: Y
authors.lastname: Ju
authors.affiliation: Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400044, People׳s Republic of China. Electronic address: song@cqu.edu.cn.
authors.forename: Guanbin
authors.initials: G
authors.lastname: Song
mesh_terms: D000199:Actins / Q000378:metabolism; D000970:Antineoplastic Agents / Q000737:chemistry; D001696:Biomechanical Phenomena; D006528:Carcinoma, Hepatocellular / Q000473:pathology; D002460:Cell Line; D002465:Cell Movement; D004354:Drug Screening Assays, Antitumor; D055119:Elastic Modulus; D006801:Humans; D007770:L-Lactate Dehydrogenase; D008113:Liver Neoplasms / Q000473:pathology; D018625:Microscopy, Atomic Force; D009362:Neoplasm Metastasis; D014411:Neoplastic Stem Cells / Q000166:cytology*; D010641:Phenotype
chemical_list: D000199:Actins; D000970:Antineoplastic Agents; D007770:L-Lactate Dehydrogenase
keywords: Atomic force microscopy; Human hepatoma cells; Liver cancer stem-like cells; Metastatic potential; Young׳s modulus