title: The Influence of α-Lipoic Acid and Garlic Administration on Biomarkers of Oxidative Stress and Inflammation in Rabbits Exposed to Oxidized Nutrition Oils.
abstract: We hypothesized that addition of substances with antioxidant activity could decrease the concentrations of biomarkers of oxidative stress and inflammatory process, thus inhibiting nonalcoholic steatohepatitis development. We investigated the influence of α-lipoic acid (ALA) and garlic administration on the development of adverse changes in rabbit liver and serum under oxidative stress conditions induced with HFD from oxidized oils. We determined 8-hydroxy-2'-deoxyguanosine (8 OHdG) and malondialdehyde (MDA) in liver homogenates, total oxidant status (TOS), lipid peroxides (LOO) and tumor necrosis factor alpha (TNFα) in blood serum, and TNFα and IL-1α genes expression in liver. The results indicate that the intake of dietary ALA and garlic was significantly associated with decreases of 8 OHdG and MDA levels in rabbits' liver tissue as well as TOS and LOO levels in rabbits' serum. Similarly, TNFα and IL-1α gene expressions were suppressed due to ALA and garlic supplementation. The histopathological analysis confirmed that HFD results in liver disorder leading to steatosis. This adverse effect of HFD was ameliorated by the supplementation of ALA and garlic. The obtained results indicate a beneficial effect of ALA and garlic administration by reducing the oxidative stress intensity and the levels of some proinflammatory cytokines in rabbits fed HFD.
authors.affiliation: Department of Biochemistry, School of Medicine with the Division of Dentistry in Zabrze, Medical University of Silesia in Katowice, Jordana 19, 41-808 Zabrze, Poland.
authors.forename: Jolanta
authors.initials: J
authors.lastname: Zalejska-Fiolka
authors.affiliation: Department of Biochemistry, School of Medicine with the Division of Dentistry in Zabrze, Medical University of Silesia in Katowice, Jordana 19, 41-808 Zabrze, Poland.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Wielkoszyński
authors.affiliation: Clinic of Ophthalmology, School of Medicine in Katowice, Medical University of Silesia in Katowice, Ceglana 35, 40-952 Katowice, Poland.
authors.forename: Wojciech
authors.initials: W
authors.lastname: Rokicki
authors.affiliation: Department of Medical and Molecular Biology, School of Medicine with the Division of Dentistry in Zabrze, Medical University of Silesia in Katowice, Jordana 19, 41-808 Zabrze, Poland.
authors.forename: Natalia
authors.initials: N
authors.lastname: Dąbrowska
authors.affiliation: Department of Medical and Molecular Biology, School of Medicine with the Division of Dentistry in Zabrze, Medical University of Silesia in Katowice, Jordana 19, 41-808 Zabrze, Poland.
authors.forename: Joanna Katarzyna
authors.initials: JK
authors.lastname: Strzelczyk
authors.affiliation: Department of Biochemistry, School of Medicine with the Division of Dentistry in Zabrze, Medical University of Silesia in Katowice, Jordana 19, 41-808 Zabrze, Poland.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Kasperczyk
authors.affiliation: Department of Statistics, SPLMS in Sosnowiec, Ostrogórska 30, 43-302 Sosnowiec, Poland.
authors.forename: Aleksander
authors.initials: A
authors.lastname: Owczarek
authors.affiliation: Department of Biochemistry, School of Medicine with the Division of Dentistry in Zabrze, Medical University of Silesia in Katowice, Jordana 19, 41-808 Zabrze, Poland.
authors.forename: Urszula
authors.initials: U
authors.lastname: Błaszczyk
authors.affiliation: Department of Biochemistry, School of Medicine with the Division of Dentistry in Zabrze, Medical University of Silesia in Katowice, Jordana 19, 41-808 Zabrze, Poland.
authors.forename: Sławomir
authors.initials: S
authors.lastname: Kasperczyk
authors.affiliation: Department of Pathology, SPLMS in Sosnowiec, Ostrogórska 30, 43-302 Sosnowiec, Poland.
authors.forename: Barbara
authors.initials: B
authors.lastname: Stawiarska-Pięta
authors.affiliation: Department of Biochemistry, School of Medicine with the Division of Dentistry in Zabrze, Medical University of Silesia in Katowice, Jordana 19, 41-808 Zabrze, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Birkner
authors.affiliation: Department of Medical Biochemistry, Wrocław Medical University, Chałubińskiego 10, 50-368 Wrocław, Poland.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Gamian
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage; D015415:Biomarkers / Q000097:blood; D016207:Cytokines / Q000276:immunology; D019587:Dietary Supplements; D005737:Garlic / Q000737:chemistry*; D007249:Inflammation / Q000139:chemically induced / Q000276:immunology* / Q000517:prevention & control; D018836:Inflammation Mediators / Q000276:immunology*; D008297:Male; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000276:immunology*; D010936:Plant Extracts / Q000008:administration & dosage*; D010938:Plant Oils; D011817:Rabbits; D008063:Thioctic Acid / Q000008:administration & dosage*
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D016207:Cytokines; D018836:Inflammation Mediators; D010936:Plant Extracts; D010938:Plant Oils; D008063:Thioctic Acid