title: Microspheres of carboxymethyl chitosan, sodium alginate and collagen for a novel hemostatic in vitro study.
abstract: To develop biocompatible composite microspheres for novel hemostatic use, we designed and prepared a novel biomaterial, composite microspheres consisting of carboxymethyl chitosan, sodium alginate, and collagen (CSCM). The ultra-structure of CSCM was investigated by scanning electron microscopy assay. In hemostatic function experiment, it was found that CSCM could facilitate platelet adherence, platelet aggregation, and platelet activation in vitro. Besides, the maximum swelling of CSCM submerged in PBS for 50 min was over 300% of that exhibited by commercial hemostatic compound microporous polysaccharide haemostatic powder (CMPHP). In addition, CSCM exhibited good biodegradability and non-cytotoxicity. These results demonstrated that CSCM may be useful in platelet plug formation, and this study would provide important information for further research on hemostasis experiment in vivo.
authors.affiliation: College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, China.
authors.forename: Xinyi
authors.initials: X
authors.lastname: Shi
authors.affiliation: College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Fang
authors.affiliation: College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, China.
authors.forename: Miao
authors.initials: M
authors.lastname: Ding
authors.affiliation: College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wu
authors.affiliation: College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, China Zhejiang Provincial Key Laboratory of Silkworm Bioreactor and Biomedicine, Hangzhou, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Ye
authors.affiliation: College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, China Zhejiang Provincial Key Laboratory of Silkworm Bioreactor and Biomedicine, Hangzhou, China.
authors.forename: Zhengbing
authors.initials: Z
authors.lastname: Lv
authors.affiliation: College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, China Zhejiang Provincial Key Laboratory of Silkworm Bioreactor and Biomedicine, Hangzhou, China aukaukauk@163.com.
authors.forename: Jia
authors.initials: J
authors.lastname: Jin
mesh_terms: D000464:Alginates / Q000737:chemistry*; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002448:Cell Adhesion; D002460:Cell Line; D002470:Cell Survival; D048271:Chitosan / Q000031:analogs & derivatives* / Q000737:chemistry; D003094:Collagen / Q000737:chemistry*; D020723:Glucuronic Acid / Q000737:chemistry; D006487:Hemostasis; D006490:Hemostatics / Q000737:chemistry*; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D051379:Mice; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D010477:Perfusion; D010973:Platelet Adhesiveness; D010974:Platelet Aggregation; D011134:Polysaccharides / Q000737:chemistry; D012867:Skin / Q000187:drug effects*; D013552:Swine
chemical_list: D000464:Alginates; D001672:Biocompatible Materials; D006490:Hemostatics; D006603:Hexuronic Acids; D011134:Polysaccharides; C514968:carboxymethyl-chitosan; D020723:Glucuronic Acid; D003094:Collagen; D048271:Chitosan
keywords: Microsphere; carboxymethyl chitosan; collagen; hemostatic; sodium alginate