title: Combining selected immunomodulatory Propionibacterium freudenreichii and Lactobacillus delbrueckii strains: Reverse engineering development of an anti-inflammatory cheese.
abstract: METHODS
  Inflammatory bowel disease (IBD) constitutes a growing public health concern in western countries. Bacteria with anti-inflammatory properties are lacking in the dysbiosis accompanying IBD. Selected strains of probiotic bacteria with anti-inflammatory properties accordingly alleviate symptoms and enhance treatment of ulcerative colitis in clinical trials. Such properties are also found in selected strains of dairy starters such as Propionibacterium freudenreichii and Lactobacillus delbrueckii (Ld). We thus investigated the possibility to develop a fermented dairy product, combining both starter and probiotic abilities of both lactic acid and propionic acid bacteria, designed to extend remissions in IBD patients.
  
  
  RESULTS
  We developed a single-strain Ld-fermented milk and a two-strain P. freudenreichii and Ld-fermented experimental pressed cheese using strains previously selected for their anti-inflammatory properties. Consumption of these experimental fermented dairy products protected mice against trinitrobenzenesulfonic acid induced colitis, alleviating severity of symptoms, modulating local and systemic inflammation, as well as colonic oxidative stress and epithelial cell damages. As a control, the corresponding sterile dairy matrix failed to afford such protection.
  
  
  CONCLUSIONS
  This work reveals the probiotic potential of this bacterial mixture, in the context of fermented dairy products. It opens new perspectives for the reverse engineering development of anti-inflammatory fermented foods designed for target populations with IBD, and has provided evidences leading to an ongoing pilot clinical study in ulcerative colitis patients.
authors.affiliation: Institut Pasteur de Lille, Lactic Acid Bacteria & Mucosal Immunity, Center for Infection and Immunity of Lille, Lille, France.
authors.forename: Coline
authors.initials: C
authors.lastname: Plé
authors.affiliation: Institut Pasteur de Lille, Lactic Acid Bacteria & Mucosal Immunity, Center for Infection and Immunity of Lille, Lille, France.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Breton
authors.affiliation: Actalia Produits Laitiers, Agrocampus Ouest, Rennes cedex, France.
authors.forename: Romain
authors.initials: R
authors.lastname: Richoux
authors.affiliation: Actalia Produits Laitiers, Agrocampus Ouest, Rennes cedex, France.
authors.forename: Marine
authors.initials: M
authors.lastname: Nurdin
authors.affiliation: Science et Technologie du Lait et de l'Œuf, INRA, Rennes, France.|Agrocampus Ouest, Rennes, France.
authors.forename: Stéphanie-Marie
authors.initials: SM
authors.lastname: Deutsch
authors.affiliation: Science et Technologie du Lait et de l'Œuf, INRA, Rennes, France.|Agrocampus Ouest, Rennes, France.
authors.forename: Hélène
authors.initials: H
authors.lastname: Falentin
authors.affiliation: Laboratoires Standa, Caen, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Hervé
authors.affiliation: Science et Technologie du Lait et de l'Œuf, INRA, Rennes, France.|Agrocampus Ouest, Rennes, France.|CIRM-BIA, Centre Internationale de Ressources Microbiennes-Bactéries d'Intérêt Alimentaire, Rennes, France.
authors.forename: Victoria
authors.initials: V
authors.lastname: Chuat
authors.affiliation: Laboratoires Standa, Caen, France.
authors.forename: Riwanon
authors.initials: R
authors.lastname: Lemée
authors.affiliation: INRA, Jouy-en-Josas, France.|AgroParisTech, UMR Micalis, Jouy-en-Josas, France.
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Maguin
authors.affiliation: Science et Technologie du Lait et de l'Œuf, INRA, Rennes, France.|Agrocampus Ouest, Rennes, France.
authors.forename: Gwénaël
authors.initials: G
authors.lastname: Jan
authors.affiliation: INRA, Jouy-en-Josas, France.|AgroParisTech, UMR Micalis, Jouy-en-Josas, France.
authors.forename: Maarten
authors.initials: M
authors.lastname: Van de Guchte
authors.affiliation: Institut Pasteur de Lille, Lactic Acid Bacteria & Mucosal Immunity, Center for Infection and Immunity of Lille, Lille, France.
authors.forename: Benoit
authors.initials: B
authors.lastname: Foligné
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D001835:Body Weight / Q000187:drug effects; D002611:Cheese / Q000382:microbiology*; D003092:Colitis / Q000139:chemically induced / Q000517:prevention & control; D003106:Colon / Q000187:drug effects / Q000473:pathology; D005260:Female; D005285:Fermentation; D000069196:Gastrointestinal Microbiome; D048668:Lactobacillus delbrueckii / Q000235:genetics / Q000276:immunology*; D008807:Mice, Inbred BALB C; D018384:Oxidative Stress / Q000187:drug effects; D019936:Probiotics / Q000494:pharmacology*; D000070002:Propionibacterium freudenreichii / Q000235:genetics / Q000276:immunology*; D014302:Trinitrobenzenesulfonic Acid / Q000633:toxicity
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D015415:Biomarkers; D014302:Trinitrobenzenesulfonic Acid
keywords: Colitis; Dairy Starter; Gut; Inflammation; Probiotic