title: Roles of phosphatidylinositol 3-kinase regulatory subunit alpha, activator protein-1, and programmed cell death 4 in diagnosis of papillary thyroid carcinoma.
abstract: This study evaluated the diagnostic values of phosphatidylinositol 3-kinase regulatory subunit alpha (P85α), activator protein-1 (AP-1), and programmed cell death 4 (PDCD4) in papillary thyroid carcinoma (PTC). P85α, AP-1, and PDCD4 expressions were detected in PTC tissues (n = 116) and thyroid papillary hyperplasia (PTH) tissues (n = 90) by immunohistochemistry, western blot, and enzyme-linked immunosorbent assay (ELISA). Associations of P85α, AP-1, and PDCD4 expressions with clinicopathological features in PTC were analyzed. Diagnostic values of P85α, AP-1, and PDCD4 in PTC were evaluated by receiver operating characteristic (ROC) curve. P85α, AP-1, and PDCD4 expression levels in PTC tissues were statistically different from those in PTH tissues (all P < 0.05). In PTC tissues, AP-1 expression was positively associated with P85α expression (r = 0.841, P < 0.01), while negatively associated with PDCD4 expression (r = -0.755, P < 0.01). P85α expression was associated with lymph node metastasis (LNM) and the degree of differentiation (both P < 0.05); AP-1 and PDCD4 expressions were associated with the degree of differentiation (both P < 0.05). The diagnostic sensitivity and specificity of P85α were 92.2 and 91.1 %, respectively, with a cutoff value of 2.100 and an area under curve (AUC) of 0.966. The diagnostic sensitivity and specificity of AP-1 reached 94.4 and 93.3 % with a cutoff value of 1.655 and an AUC of 0.987. The diagnostic sensitivity and specificity of PDCD4 were 54.4 and 85.6 % with a cutoff value of 2.025 and an AUC of 0.754. P85α, AP-1, and PDCD4 proteins may be related to the tumorigenesis and progression of PTC. Moreover, P85α, AP-1, and PDCD4 proteins may serve as potential diagnostic markers to the biological behavior of PTC.
authors.affiliation: Department of Endocrinology, First Affiliated Hospital of Wenzhou Medical University, Ouhai District, Wenzhou, 325015, Zhejiang, People's Republic of China.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Endocrinology, First Affiliated Hospital of Wenzhou Medical University, Ouhai District, Wenzhou, 325015, Zhejiang, People's Republic of China.
authors.forename: Wenjun
authors.initials: W
authors.lastname: Wu
authors.affiliation: Department of Endocrinology, First Affiliated Hospital of Wenzhou Medical University, Ouhai District, Wenzhou, 325015, Zhejiang, People's Republic of China. chenxiong1008@yeah.net.
authors.forename: Xiong
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Endocrinology, First Affiliated Hospital of Wenzhou Medical University, Ouhai District, Wenzhou, 325015, Zhejiang, People's Republic of China. gongxiaohua1008@126.com.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Gong
mesh_terms: D000293:Adolescent; D000328:Adult; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism*; D014408:Biomarkers, Tumor; D002277:Carcinoma / Q000175:diagnosis* / Q000378:metabolism*; D002291:Carcinoma, Papillary; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D060787:Neoplasm Grading; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000378:metabolism*; D012372:ROC Curve; D000077273:Thyroid Cancer, Papillary; D013964:Thyroid Neoplasms / Q000175:diagnosis* / Q000378:metabolism*; D018808:Transcription Factor AP-1 / Q000378:metabolism*; D055815:Young Adult
chemical_list: D051017:Apoptosis Regulatory Proteins; D014408:Biomarkers, Tumor; C114487:PDCD4 protein, human; D016601:RNA-Binding Proteins; D018808:Transcription Factor AP-1; D019869:Phosphatidylinositol 3-Kinases
keywords: AP-1; Diagnosis; Immunohistochemistry; P85α; PDCD4; Papillary thyroid carcinoma; ROC curve; Tumorigenesis