title: Erythrocyte and platelet proteomics in hematological disorders.
abstract: Erythrocytes undergo ineffective erythropoesis, hemolysis, and premature eryptosis in sickle cell disease and thalassemia. Abnormal hemoglobin variants associated with hemoglobinopathy lead to vesiculation, membrane instability, and loss of membrane asymmetry with exposal of phosphatidylserine. This potentiates thrombin generation resulting in activation of the coagulation cascade responsible for subclinical phenotypes. Platelet activation also results in the release of microparticles, which express and transfer functional receptors from platelet membrane, playing key roles in vascular reactivity and activation of intracellular signaling pathways. Over the last decade, proteomics had proven to be an important field of research in studies of blood and blood diseases. Blood cells and its fluidic components have been proven to be easy systems for studying differential expressions of proteins in hematological diseases encompassing hemoglobinopathies, different types of anemias, myeloproliferative disorders, and coagulopathies. Proteomic studies of erythrocytes and platelets reported from several groups have highlighted various factors that intersect the signaling networks in these anucleate systems. In this review, we have elaborated on the current scenario of anucleate blood cell proteomes in normal and diseased individuals and the cross-talk between the two major constituent cell types of circulating blood.
authors.affiliation: Crystallography and Molecular Biology Division, Saha Institute of Nuclear Physics, Kolkata, India.
authors.forename: Abhijit
authors.initials: A
authors.lastname: Chakrabarti
authors.affiliation: Crystallography and Molecular Biology Division, Saha Institute of Nuclear Physics, Kolkata, India.
authors.forename: Suchismita
authors.initials: S
authors.lastname: Halder
authors.affiliation: Biophysics and Structural Genomics Division, Saha institute of Nuclear Physics, Kolkata, India.
authors.forename: Shilpita
authors.initials: S
authors.lastname: Karmakar
mesh_terms: D000755:Anemia, Sickle Cell / Q000097:blood* / Q000175:diagnosis / Q000473:pathology; D001792:Blood Platelets / Q000737:chemistry* / Q000378:metabolism / Q000473:pathology; D055252:Cell-Derived Microparticles / Q000737:chemistry / Q000378:metabolism; D000072817:Eryptosis; D004912:Erythrocytes / Q000737:chemistry* / Q000378:metabolism / Q000473:pathology; D006455:Hemoglobins, Abnormal / Q000737:chemistry* / Q000378:metabolism; D006461:Hemolysis; D006801:Humans; D010718:Phosphatidylserines / Q000378:metabolism; D015539:Platelet Activation; D040901:Proteomics / Q000379:methods*; D015398:Signal Transduction; D013789:Thalassemia / Q000097:blood* / Q000175:diagnosis / Q000473:pathology; D013917:Thrombin / Q000378:metabolism
chemical_list: D006455:Hemoglobins, Abnormal; D010718:Phosphatidylserines; D013917:Thrombin
keywords: Erythrocytes; Hematological disorders; Interactome; Platelets; Proteomics