title: HPV Infection, but Not EBV or HHV-8 Infection, Is Associated with Salivary Gland Tumours.
abstract: Benign and malignant salivary gland tumours are clinically heterogeneous and show different histology. Little is known about the role of human herpes virus 8 (HHV-8), Epstein-Barr virus (EBV), and human papillomavirus (HPV) infection in salivary gland neoplasms. We investigated the presence of the three viruses in formalin-fixed, paraffin-embedded tissue samples in a cohort of 200 different salivary gland tumours. We performed EBV-LMP-1 and HHV-8 and p16 immunohistochemistry, a specific chip based hybridization assay for detection and typing of HPV and a chromogenic in situ hybridization for EBV analysis. Only one case, a polymorphic low-grade carcinoma, showed HHV-8 expression and one lymphoepithelial carcinoma was infected by EBV. In 17 cases (9%) moderate or strong nuclear and cytoplasmic p16 expression was detected. The HPV type was investigated in all of these cases and additionally in 8 Warthin's tumours. In 19 cases HPV type 16 was detected, mostly in Warthin's tumour, adenoid cystic carcinoma, and adenocarcinoma NOS. We concluded that HHV-8 infection and EBV infection are not associated with salivary gland cancer, but HPV infection may play a role in these tumour entities.
authors.affiliation: Institute of Pathology, University of Rostock, Strempelstrasse 14, 18055 Rostock, Germany.
authors.forename: Maja
authors.initials: M
authors.lastname: Hühns
authors.affiliation: Institute of Pathology, University of Rostock, Strempelstrasse 14, 18055 Rostock, Germany.
authors.forename: Georg
authors.initials: G
authors.lastname: Simm
authors.affiliation: Institute of Pathology, University of Rostock, Strempelstrasse 14, 18055 Rostock, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Erbersdobler
authors.affiliation: Institute of Pathology, University of Rostock, Strempelstrasse 14, 18055 Rostock, Germany ; Institute of Pathology, University Medical Centre Jena, Ziegelmühlenweg 1, 07743 Jena, Germany.
authors.forename: Annette
authors.initials: A
authors.lastname: Zimpfer
mesh_terms: D000235:Adenolymphoma / Q000821:virology; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D004279:DNA, Viral / Q000235:genetics; D020031:Epstein-Barr Virus Infections / Q000150:complications* / Q000821:virology; D005260:Female; D006566:Herpesviridae Infections / Q000150:complications* / Q000821:virology; D004854:Herpesvirus 4, Human / Q000472:pathogenicity; D019288:Herpesvirus 8, Human / Q000472:pathogenicity; D006801:Humans; D008297:Male; D008875:Middle Aged; D027383:Papillomaviridae / Q000472:pathogenicity*; D030361:Papillomavirus Infections / Q000150:complications* / Q000821:virology; D012468:Salivary Gland Neoplasms / Q000209:etiology* / Q000821:virology*; D012469:Salivary Glands / Q000821:virology*; D055815:Young Adult
chemical_list: D004279:DNA, Viral