title: OBTAINING OF THE TRANSGENIC HELIANTHUS TUBEROSUS L. PLANTS, CALLUS AND "HAIRY" ROOT CULTURES ABLE TO EXPRESS THE RECOMBINANT HUMAN INTERFERON ALPHA-2b GENE.
abstract: This work is the first to our knowledge to describe the successful attempt of Agrobacterium rhizogenes-mediated transformation of topinambour in order to obtain the transgenic H. tuberosus plants, callus and "hairy" root cultures. The plasmid vectors contained the sequence of interferon gene fused with Nicotiana plumbagenifolia L. calreticulin apoplast targeting signal driven by 35S CaMV promoter or root-specific Mll promoter. Nearly 75% isolated Ri-root lines and callus cultures were proved (by PCR analysis) to contain HuINFa-2b transgene. We also managed to obtain H. tuberosus transgenic plants through somatic embryogenesis on the transgenic "hairy" root culture. The obtained transgenic H. tuberosus cultures exhibited high-level antiviral activity that ranged from 2000 to 54500 IU/g FW that makes this crop considered a promising source of recombinant interferon alpha 2b protein.
authors.forename: O M
authors.initials: OM
authors.lastname: Maistrenko
authors.forename: Yu S
authors.initials: YS
authors.lastname: Luchakivska
authors.forename: N M
authors.initials: NM
authors.lastname: Zholobak
authors.forename: M Ya
authors.initials: MY
authors.lastname: Spivak
authors.forename: M V
authors.initials: MV
authors.lastname: Kuchuk
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics*; D000818:Animals; D000998:Antiviral Agents / Q000302:isolation & purification* / Q000494:pharmacology; D002460:Cell Line; D004847:Epithelial Cells / Q000187:drug effects / Q000276:immunology / Q000821:virology; D005822:Genetic Vectors / Q000737:chemistry / Q000378:metabolism; D006368:Helianthus / Q000235:genetics* / Q000378:metabolism / Q000382:microbiology; D006801:Humans; D007155:Immunologic Factors / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology; D000077190:Interferon alpha-2; D016898:Interferon-alpha / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology; D008297:Male; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism / Q000382:microbiology; D058439:Plant Somatic Embryogenesis Techniques / Q000379:methods; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D021382:Protein Sorting Signals; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology; D013552:Swine; D013737:Testis / Q000187:drug effects / Q000276:immunology / Q000821:virology; D046509:Tissue Culture Techniques; D014026:Nicotiana / Q000737:chemistry / Q000235:genetics; D014721:Vesicular stomatitis Indiana virus / Q000187:drug effects / Q000254:growth & development
chemical_list: D000998:Antiviral Agents; D007155:Immunologic Factors; D000077190:Interferon alpha-2; D016898:Interferon-alpha; D021382:Protein Sorting Signals; D011994:Recombinant Proteins