title: Erythropoietin Inhibits the Increase of Pulmonary Labile Zinc and the Expression of Inflammatory Mediators Following Subarachnoid Hemorrhage in Rats.
abstract: Aneurysmal subarachnoid hemorrhage (SAH) is a common condition with relatively poor clinical outcome. Pulmonary complication after SAH is an important contributor to poor outcome. Previous studies have shown that labile zinc and inflammatory mediators participate in many pathophysiological processes. The present study investigated the effects of SAH on the levels of labile zinc and certain proinflammatory factors in rat lung and determined the effect of erythropoietin (EPO) on the pulmonary labile zinc and the inflammatory factor after SAH in rats.
  
  
  
  Experiment 1 aimed to investigate the time course of increase of pulmonary labile zinc, wet/dry weight ratio, and the expression of inflammatory mediators after SAH. In Experiment 2, we chose the maximum time point which lung injury was maximally severity and assessed the effect of EPO on regulation of the pulmonary labile zinc, inflammatory reaction, and wet/dry weight ratio after SAH.
  
  
  
  SAH caused a gradual increase of pulmonary labile zinc as demonstrated by fluorescence staining with Zinpyr-4. The levels of TNF-α and IL-8 and the lung wet/dry weight ratios were higher in the SAH groups compared to the control group and peaked on 3 days following SAH (p < 0.05). EPO significantly reduced the pulmonary labile zinc, the inflammatory mediators, and the lung wet/dry weight ratio compared with SAH group (p < 0.05).
  
  
  
  EPO can protect lung from SAH-induced injury by attenuating pulmonary inflammation and labile zinc accumulation in vivo.
authors.affiliation: Department of Radiology, Wuxi Integrated Traditional Chinese and Western Medicine Hospital, 585 North Xingyuan Road, Wuxi, 214041, Jiangsu, People's Republic of China.
authors.forename: Yiting
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Department of Neurosurgery, Wuxi Second Hospital Affiliated Nanjing Medical University, 68 Zhong Shan Road, Wuxi, 214002, Jiangsu, People's Republic of China.
authors.forename: Xudong
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Department of Neurosurgery, Wuxi Second Hospital Affiliated Nanjing Medical University, 68 Zhong Shan Road, Wuxi, 214002, Jiangsu, People's Republic of China.
authors.forename: Yanna
authors.initials: Y
authors.lastname: Tang
authors.affiliation: Department of Neurosurgery, School of Medicine, Jinling Hospital, Nanjing University, 305 East Zhongshan Road, Nanjing, 210002, Jiangsu, People's Republic of China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhu
authors.affiliation: Department of Radiology, Wuxi Integrated Traditional Chinese and Western Medicine Hospital, 585 North Xingyuan Road, Wuxi, 214041, Jiangsu, People's Republic of China. jiangjianwei1964@163.com.
authors.forename: Jianwei
authors.initials: J
authors.lastname: Jiang
authors.affiliation: Department of Neurosurgery, Wuxi Second Hospital Affiliated Nanjing Medical University, 68 Zhong Shan Road, Wuxi, 214002, Jiangsu, People's Republic of China. luxiaojie1966@aliyun.com.
authors.forename: Xiaojie
authors.initials: X
authors.lastname: Lu
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004921:Erythropoietin / Q000494:pharmacology*; D018836:Inflammation Mediators / Q000378:metabolism*; D008168:Lung / Q000378:metabolism* / Q000473:pathology; D055370:Lung Injury / Q000378:metabolism* / Q000517:prevention & control; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013345:Subarachnoid Hemorrhage / Q000378:metabolism*; D015032:Zinc / Q000378:metabolism*
chemical_list: D018836:Inflammation Mediators; D004921:Erythropoietin; D015032:Zinc
keywords: Erythropoietin; Inflammatory mediators; Subarachnoid hemorrhage; Zinc