title: Gene expression profiling of taxol-resistant nasopharyngeal carcinoma cells with siRNA-mediated FOLR1 downregulation.
abstract: OBJECTIVE
  Our previous study has shown that downregulation of FOLR1 by siRNA partially reversed taxol-resistant phenotype in taxol-resistant nasopharyngeal carcinoma cell lines. We aim to gain further insight into the molecular mechanisms of this process and identify the differentially expressed genes after FOLR1 downregulation.
  
  
  METHODS
  The global gene expression profile was identified and analyzed using the Affymetrix HG-U133 Plus 2.0 array.
  
  
  RESULTS
  There was a significant dysregulation in the global gene expression of the FOLR1-suppressed taxol-resistant nasopharyngeal carcinoma cell lines. There were 41 upregulated genes and 109 downregulated genes. QRT-PCR validation of the selected differentially expressed genes demonstrated there was a good correlation with the microarray analysis. There was a significant deregulation of expression in the apoptosis-related genes such as BIRC3, PRKX, TNFRSF10A and involved in Viral carcinogenesis, MAPK signaling pathways after FOLR1 was downregulated.
  
  
  CONCLUSIONS
  The suppression of FOLR1 by RNA interference altered gene expression profile of taxol-resistant nasopharyngeal carcinoma cell lines. The apoptosis-related genes and the gene alterations in viral carcinogenesis, MAPK signaling pathways might be important in FOLR1 siRNA-induced taxol-resistant reversal.
authors.affiliation: Department of Otolaryngology-Head Neck Surgery, The Third Xiangya Hospital of Central South University Changsha, Hunan, China.
authors.forename: Yexun
authors.initials: Y
authors.lastname: Song
authors.affiliation: Department of Head and Neck Surgery and Oncology Plastic Surgery, The Affiliated Cancer Hospital of Xiangya Medical School, Central South University Changsha, Hunan, China.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Peng
authors.affiliation: Department of Otolaryngology-Head Neck Surgery, The Hunan Children's Hospital Changsha, Hunan, China.
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.affiliation: Department of Otolaryngology-Head Neck Surgery, The Hunan Children's Hospital Changsha, Hunan, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Xie
authors.affiliation: Department of Otolaryngology-Head Neck Surgery, The Third Xiangya Hospital of Central South University Changsha, Hunan, China.
authors.forename: Guolin
authors.initials: G
authors.lastname: Tan
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000235:genetics; D045744:Cell Line, Tumor; D002472:Cell Transformation, Viral / Q000235:genetics; D016000:Cluster Analysis; D015536:Down-Regulation; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D058975:Folate Receptor 1 / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling / Q000379:methods; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D020935:MAP Kinase Signaling System / Q000235:genetics; D009303:Nasopharyngeal Neoplasms / Q000188:drug therapy* / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D020411:Oligonucleotide Array Sequence Analysis; D017239:Paclitaxel / Q000494:pharmacology*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics* / Q000378:metabolism; D014162:Transfection
chemical_list: D000972:Antineoplastic Agents, Phytogenic; C548696:FOLR1 protein, human; D058975:Folate Receptor 1; D034741:RNA, Small Interfering; D017239:Paclitaxel
keywords: FOLR1; Gene expression profiling; nasopharyngeal carcinoma; signaling pathway; small interfering RNA; taxol resistance