title: MiR-19a targets suppressor of cytokine signaling 1 to modulate the progression of neuropathic pain.
abstract: OBJECTIVE
  We aimed to investigate whether miR-19a is associated with neuropathic pain and elucidate the underlying regulatory mechanism.
  
  
  METHODS
  We established a neuropathic pain model of bilateral chronic constriction injury (bCCI). Then bCCI rats were injected with mo-miR-19a, siR-SOCS1 or blank expression vector through a microinjection syringe via an intrathecal catheter on 3 day before surgery and after surgery. Behavioral tests, such as mechanical allodynia, thermal hyperalgesia and acetone induced cold allodynia, were performed to evaluate the pain threshold. Besides, quantitative real-time polymerase chain reaction (qRT-PCR) was performed to determine the expression of miR-19a and western blotting was carried out to measure the expression of SOCS1.
  
  
  RESULTS
  miR-19a expression levels were markedly increased in neuropathic pain models. Moreover, miR-19a significantly attenuated mechanical allodynia and thermal hyperalgesia, and similar results were obtained after knockdown of SOCS1 expression. However, miR-19a markedly increased the times that the rats appeared a sign of cold allodynia, and knockdown of SOCS1 expression had similar effects. Besides, the results of bioinformatics analysis and western blotting analysis were all confirmed that SOCS1 was a direct target of miR-19a in neuropathic pain models.
  
  
  CONCLUSIONS
  Our finding indicate that SOCS1 is a direct target of miR-19a in neuropathic pain rats and miR-19a may play a critical role in regulating of neuropathic pain via targeting SOCS1.
authors.affiliation: Department of Anesthesiology, The First Hospital of Xiangyang City Xiangyang 441000, Hubei Province, China.
authors.forename: Conghui
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Anesthesiology, The First Hospital of Xiangyang City Xiangyang 441000, Hubei Province, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Jiang
authors.affiliation: Department of Anesthesiology, The First Hospital of Xiangyang City Xiangyang 441000, Hubei Province, China.
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.affiliation: Department of Anesthesiology, The First Hospital of Xiangyang City Xiangyang 441000, Hubei Province, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Li
mesh_terms: D000818:Animals; D015153:Blotting, Western; D004195:Disease Models, Animal; D018450:Disease Progression; D005786:Gene Expression Regulation / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D009437:Neuralgia / Q000235:genetics* / Q000378:metabolism; D017288:Pain Threshold; D034741:RNA, Small Interfering; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D000071222:Suppressor of Cytokine Signaling 1 Protein; D050826:Suppressor of Cytokine Signaling Proteins / Q000096:biosynthesis / Q000235:genetics*; D059467:Transcriptome
chemical_list: C586754:MIRN19 microRNA, rat; D035683:MicroRNAs; D034741:RNA, Small Interfering; C491589:Socs1 protein, rat; D000071222:Suppressor of Cytokine Signaling 1 Protein; D050826:Suppressor of Cytokine Signaling Proteins
keywords: Neuropathic pain; cold allodynia; mechanical allodynia; miR-19a; suppressor of cytokine signaling 1; thermal hyperalgesia