title: The Expression of Notch 1 and Notch 3 in Gallbladder Cancer and Their Clinicopathological Significance.
abstract: Gallbladder cancers (GBCs) are highly malignant gastrointestinal cancers. The biological makers for the prognosis and targeting therapy of GBCs have not been established. The protein expression of Notch 1 and Notch 3 in 46 squamous cell/adenosquamous carcinomas (SC/ASCs) and 80 adenocarcinomas (AC) was measured using immunohistochemistry. Positive Notch 1 and Notch 3 expression in both SC/ASC and AC was significantly associated with large tumor size, invasion, metastasis, and low surgical curability (P < 0.05 or P < 0.01). Univariate Kaplan-Meier analysis showed that positive Notch 1 and Notch 3 expression was significantly associated with mean survival of SC/ASC and AC patients (P < 0.01 or P < 0.001). Multivariate Cox regression analysis showed that positive Notch 1 and Notch 3 expression, as well as low differentiation, large tumor size, high TNM stage, invasion, lymph node metastasis, and surgical curability are independent poor-prognostic factors in both SC/ASC and AC patients. Positive Notch 1 and Notch 3 expression is closely correlated with severe clinicopathological characteristics and poor prognosis in both SC/ASC and AC patients.
authors.affiliation: Research Laboratory of Hepatobiliary Diseases, Second Xiangya Hospital, Central South University, Changsha, Hunan, 410011, People's Republic of China.
authors.forename: Luyao
authors.initials: L
authors.lastname: Liu
authors.affiliation: Research Laboratory of Hepatobiliary Diseases, Second Xiangya Hospital, Central South University, Changsha, Hunan, 410011, People's Republic of China. zhulinyang@yahoo.com.
authors.forename: Zhu-Lin
authors.initials: ZL
authors.lastname: Yang
authors.affiliation: Research Laboratory of Hepatobiliary Diseases, Second Xiangya Hospital, Central South University, Changsha, Hunan, 410011, People's Republic of China.
authors.forename: Chunwei
authors.initials: C
authors.lastname: Wang
authors.affiliation: Research Laboratory of Hepatobiliary Diseases, Second Xiangya Hospital, Central South University, Changsha, Hunan, 410011, People's Republic of China.
authors.forename: Xiongying
authors.initials: X
authors.lastname: Miao
authors.affiliation: Research Laboratory of Hepatobiliary Diseases, Second Xiangya Hospital, Central South University, Changsha, Hunan, 410011, People's Republic of China.
authors.forename: Zhiyu
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Pathology, Second Xiangya Hospital, Central South University, Changsha, Hunan, 410011, People's Republic of China.
authors.forename: Daiqiang
authors.initials: D
authors.lastname: Li
authors.affiliation: Department of Pathology, Third Xiangya Hospital, Central South University, Changsha, Hunan, 410013, People's Republic of China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Zou
authors.affiliation: Department of Pathology, Basic School of Medicine, Central South University, Changsha, Hunan, 410078, People's Republic of China.
authors.forename: Jinghe
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Hepatobiliary and Pancreatic Surgery, Hunan Provincial People's Hospital, Changsha, Hunan, 410007, People's Republic of China.
authors.forename: Lufeng
authors.initials: L
authors.lastname: Liang
authors.affiliation: Department of Pathology, Loudi Central Hospital, Loudi, Hunan, 417011, People's Republic of China.
authors.forename: Guixiang
authors.initials: G
authors.lastname: Zeng
authors.affiliation: Department of Pathology, Hunan Provincial Tumor Hospital, Changsha, Hunan, 410013, People's Republic of China.
authors.forename: Senlin
authors.initials: S
authors.lastname: Chen
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology; D014408:Biomarkers, Tumor / Q000378:metabolism; D018196:Carcinoma, Adenosquamous / Q000378:metabolism / Q000473:pathology; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000473:pathology; D002454:Cell Differentiation / Q000502:physiology; D005260:Female; D005706:Gallbladder Neoplasms / Q000378:metabolism* / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D053208:Kaplan-Meier Estimate; D008207:Lymphatic Metastasis / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging / Q000379:methods; D011379:Prognosis; D051881:Receptor, Notch1 / Q000378:metabolism*; D000071656:Receptor, Notch3 / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; C497761:NOTCH1 protein, human; C499374:NOTCH3 protein, human; D051881:Receptor, Notch1; D000071656:Receptor, Notch3
keywords: Adenocarcinoma; Gallbladder cancer; Notch 1; Notch 3; Prognosis; Squamous cell/adenosquamous carcinomas