title: Evaluation of spheroid head and neck squamous cell carcinoma cell models in comparison to monolayer cultures.
abstract: Two-dimensional (2D) monolayer cell culture models are the most common method used to investigate tumor cellsin vitro. In the few last decades, a multicellular spheroid model has gained attention due to its adjacency to tumorsin vivo. The aim of the present study was to investigate immunohistochemical differences between these two cell culture systems. The FaDu, CAL27 and SCC25 head and neck squamous cell carcinoma (HNSCC) cell lines were seeded out in monolayer and multicellular spheroids. The FaDu and SCC25 cells were treated with increasing doses of cisplatin and irradiation. CAL27 cells were not used in theproliferation experiments, since the spheroids of CAL27 cells were not able to process the reagent in CCK-8 assays. Furthermore, they were stained to present alterations of the following antigens: Ki-67, vascular endothelial growth factor receptor, epithelial growth factor and survivin. Differences in growth rates and expression patterns were detected in certain HNSCC cell lines. The proliferation rates showed a significant divergence of cells grown in the three-dimensional model compared with cells grown in the 2D model. Overall, multicellular spheroids are a promising method to reproduce the immunohistochemical aspects and characteristics of tumor cells, and may show different response rates to therapeutic options.
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, Medical University of Vienna, Vienna A-1090, Austria.
authors.forename: Lorenz
authors.initials: L
authors.lastname: Kadletz
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, Medical University of Vienna, Vienna A-1090, Austria.
authors.forename: Gregor
authors.initials: G
authors.lastname: Heiduschka
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, Medical University of Vienna, Vienna A-1090, Austria.
authors.forename: Julian
authors.initials: J
authors.lastname: Domayer
authors.affiliation: Department of Radiotherapy, Medical University of Vienna, Vienna A-1090, Austria.
authors.forename: Rainer
authors.initials: R
authors.lastname: Schmid
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, Medical University of Vienna, Vienna A-1090, Austria.
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Enzenhofer
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, Medical University of Vienna, Vienna A-1090, Austria.
authors.forename: Dietmar
authors.initials: D
authors.lastname: Thurnher
keywords: cell culture; cisplatin; head and neck squamous cell carcinoma; irradiation; multicellular spheroids