title: Resources for the design of CRISPR gene editing experiments.
abstract: CRISPR-based approaches have quickly become a favored method to perturb genes to uncover their functions. Here, we review the key considerations in the design of genome editing experiments, and survey the tools and resources currently available to assist users of this technology.
authors.affiliation: Broad Institute of MIT and Harvard, Cambridge, MA, 02142, USA.|Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA, 02114, USA.|Center for the Study of Inflammatory Bowel Disease, Massachusetts General Hospital, Boston, MA, 02114, USA.
authors.forename: Daniel B
authors.initials: DB
authors.lastname: Graham
authors.affiliation: Broad Institute of MIT and Harvard, Cambridge, MA, 02142, USA. droot@broadinstitute.org.
authors.forename: David E
authors.initials: DE
authors.lastname: Root
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D000076987:CRISPR-Associated Protein 9; D064113:CRISPR-Cas Systems / Q000235:genetics*; D004720:Endonucleases / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D005786:Gene Expression Regulation; D018390:Gene Targeting; D017393:RNA Editing / Q000235:genetics*; D000094704:RNA, Guide, CRISPR-Cas Systems / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D000094704:RNA, Guide, CRISPR-Cas Systems; D000076987:CRISPR-Associated Protein 9; C000606107:Cas9 endonuclease Streptococcus pyogenes; D004720:Endonucleases