title: [Expression of cMPO in 502 cases of acute myeloid leukemia (AML) and its diagnosis significance in AML subtypes].
abstract: OBJECTIVE
  To investigate the myeloperoxidase (cMPO) expression pattern by flow cytometry (FCM) in patients with acute myeloid leukemia (AML) and its role in classifying AML.
  
  
  METHODS
  Eight- color multiparametric FCM with CD45/SSC gating was used to determine the cMPO expression in 502 AML patients.
  
  
  RESULTS
  The positive rate of cMPO in all patients was 58.0%, in which the proportion of normal positivity, dim positivity and partial positivity was 21.5%, 34.1% and 2.4%, respectively. The remaining case (42.0%) were all negative. In AML with t (15;17）(q22;q12)/PMLRARα, the positive rate was the highest (100%) and the intensity was similar to that of the normal granular leukocytes, followed by AML with t (8;21（q22;q22/RUNX1-RUNX1T1, the positive rate was 91.4% and the intensity was mostly dim. AML with minimal differentiation and acute megakaryoblastic leukemia were all cMPO negative. The positive rates of cMPO in the remaining subtypes were between 22.7% and 76.2%.
  
  
  CONCLUSIONS
  The positive rate and intensity of cMPO were significantly different among different subtypes of AML.
authors.affiliation: Institute of Hematology and Blood Disease Hospital, CAMS & PUMC, Tianjin 300020, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Shang
authors.affiliation: Institute of Hematology and Blood Disease Hospital, CAMS & PUMC, Tianjin 300020, China.
authors.forename: Xuejing
authors.initials: X
authors.lastname: Chen
authors.affiliation: Institute of Hematology and Blood Disease Hospital, CAMS & PUMC, Tianjin 300020, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Institute of Hematology and Blood Disease Hospital, CAMS & PUMC, Tianjin 300020, China.
authors.forename: Guiqing
authors.initials: G
authors.lastname: Guo
authors.affiliation: Institute of Hematology and Blood Disease Hospital, CAMS & PUMC, Tianjin 300020, China.
authors.forename: Dashui
authors.initials: D
authors.lastname: He
authors.affiliation: Institute of Hematology and Blood Disease Hospital, CAMS & PUMC, Tianjin 300020, China.
authors.forename: Xiaojin
authors.initials: X
authors.lastname: Cai
authors.affiliation: Institute of Hematology and Blood Disease Hospital, CAMS & PUMC, Tianjin 300020, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zheng
authors.affiliation: Institute of Hematology and Blood Disease Hospital, CAMS & PUMC, Tianjin 300020, China.
authors.forename: Yingchang
authors.initials: Y
authors.lastname: Mi
authors.affiliation: Institute of Hematology and Blood Disease Hospital, CAMS & PUMC, Tianjin 300020, China.
authors.forename: Jianxiang
authors.initials: J
authors.lastname: Wang
authors.affiliation: Institute of Hematology and Blood Disease Hospital, CAMS & PUMC, Tianjin 300020, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Ru
authors.affiliation: Institute of Hematology and Blood Disease Hospital, CAMS & PUMC, Tianjin 300020, China.
authors.forename: Huijun
authors.initials: H
authors.lastname: Wang
mesh_terms: D002454:Cell Differentiation; D005434:Flow Cytometry; D006098:Granulocytes; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000145:classification / Q000235:genetics*; D009195:Peroxidase / Q000235:genetics*
chemical_list: D009195:Peroxidase