title: Correlations between MTP and ROS Levels of Peripheral Blood Lymphocytes and Readmission in Patients with Chronic Heart Failure.
abstract: BACKGROUND
  Peripheral blood lymphocytes exhibit changes that parallel those in failing cardiomyocytes. We hypothesised that mitochondrial transmembrane potential (MTP) and reactive oxygen species (ROS) levels of lymphocytes are associated with serum NT-proBNP and short-term prognosis in patients with chronic heart failure (CHF).
  
  
  METHODS
  Fifty-four CHF patients and 30 controls were enrolled in this prospective study. Mitochondrial transmembrane potential and ROS levels of lymphocytes were evaluated by flow cytometry and reported as the JC-1 fluorescence ratio and DCF fluorescence intensity, respectively. Serum NT-proBNP levels and biochemical parameters were also examined. All the participants received follow-up to evaluate clinical end-points after eight months.
  
  
  RESULTS
  Chronic heart failure patients had higher levels of DCF fluorescence intensity of lymphocytes and serum NT-proBNP, as well as lower levels of JC-1 fluorescence ratios compared with those of controls (all P<0.05). A closer relationship was found between Lg(DCF fluorescence intensity of lymphocytes) or JC-1 fluorescence ratio of lymphocytes and Lg(NT-proBNP) (both P<0.05) in CHF patients. During the eight-month follow-up period, 14 CHF patients (25.9%) were readmitted for severe HF, but none died. A logistic regression analysis showed that both ROS level and MTP of the lymphocytes were independent predictors (B=7.03, P=0.006; B= - 0.32, P=0.029, respectively) of readmission of CHF patients.
  
  
  CONCLUSIONS
  In CHF patients at low risk, MTP and ROS levels of the lymphocytes showed a significant change that is associated with serum NT-proBNP and patient readmission.
authors.affiliation: Department of Cardiovascular Medicine, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China; Shaanxi Key Laboratory of Molecular Cardiology (Xi'an Jiaotong University), Xi'an, Shaanxi, China; Key Laboratory of Environment and Genes Related to Diseases (Xi'an Jiaotong University), Ministry of Education, Xi'an, Shaanxi, China.
authors.forename: Bingxue
authors.initials: B
authors.lastname: Song
authors.affiliation: Department of Cardiovascular Medicine, Xi'an Central Hospital, Xi'an, Shaanxi, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Li
authors.affiliation: Department of Cardiovascular Medicine, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China; Shaanxi Key Laboratory of Molecular Cardiology (Xi'an Jiaotong University), Xi'an, Shaanxi, China; Key Laboratory of Environment and Genes Related to Diseases (Xi'an Jiaotong University), Ministry of Education, Xi'an, Shaanxi, China.
authors.forename: Shuping
authors.initials: S
authors.lastname: Chen
authors.affiliation: Department of Cardiovascular Medicine, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China; Shaanxi Key Laboratory of Molecular Cardiology (Xi'an Jiaotong University), Xi'an, Shaanxi, China; Key Laboratory of Environment and Genes Related to Diseases (Xi'an Jiaotong University), Ministry of Education, Xi'an, Shaanxi, China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Yang
authors.affiliation: Department of Cardiovascular Medicine, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China; Shaanxi Key Laboratory of Molecular Cardiology (Xi'an Jiaotong University), Xi'an, Shaanxi, China; Key Laboratory of Environment and Genes Related to Diseases (Xi'an Jiaotong University), Ministry of Education, Xi'an, Shaanxi, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Luo
authors.affiliation: Department of Cardiovascular Medicine, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China; Shaanxi Key Laboratory of Molecular Cardiology (Xi'an Jiaotong University), Xi'an, Shaanxi, China; Key Laboratory of Environment and Genes Related to Diseases (Xi'an Jiaotong University), Ministry of Education, Xi'an, Shaanxi, China.
authors.forename: Tingzhong
authors.initials: T
authors.lastname: Wang
authors.affiliation: Department of Cardiovascular Medicine, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China; Shaanxi Key Laboratory of Molecular Cardiology (Xi'an Jiaotong University), Xi'an, Shaanxi, China; Key Laboratory of Environment and Genes Related to Diseases (Xi'an Jiaotong University), Ministry of Education, Xi'an, Shaanxi, China.
authors.forename: Xiu
authors.initials: X
authors.lastname: Han
authors.affiliation: Department of Cardiovascular Medicine, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China; Shaanxi Key Laboratory of Molecular Cardiology (Xi'an Jiaotong University), Xi'an, Shaanxi, China; Key Laboratory of Environment and Genes Related to Diseases (Xi'an Jiaotong University), Ministry of Education, Xi'an, Shaanxi, China. Electronic address: bailingb21@sina.com.
authors.forename: Ling
authors.initials: L
authors.lastname: Bai
authors.affiliation: Department of Cardiovascular Medicine, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China; Shaanxi Key Laboratory of Molecular Cardiology (Xi'an Jiaotong University), Xi'an, Shaanxi, China; Key Laboratory of Environment and Genes Related to Diseases (Xi'an Jiaotong University), Ministry of Education, Xi'an, Shaanxi, China. Electronic address: maaiqun@medmail.com.cn.
authors.forename: Aiqun
authors.initials: A
authors.lastname: Ma
mesh_terms: D000368:Aged; D002908:Chronic Disease; D005260:Female; D006333:Heart Failure / Q000097:blood*; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism* / Q000473:pathology; D008297:Male; D053078:Membrane Potential, Mitochondrial; D008875:Middle Aged; D010359:Patient Readmission; D011237:Predictive Value of Tests; D011446:Prospective Studies; D017382:Reactive Oxygen Species / Q000097:blood*
chemical_list: D017382:Reactive Oxygen Species
keywords: Chronic heart failure; Mitochondrial transmembrane potential; N-terminal-probrain natriuretic peptide; Reactive oxygen species; Readmission