title: Multiscale Origami Structures as Interface for Cells.
abstract: A DNA-based platform was developed to address fundamental aspects of early stages of cell signaling in living cells. By site-directed sorting of differently encoded, protein-decorated DNA origami structures on DNA microarrays, we combine the advantages of the bottom-up self-assembly of protein-DNA nanostructures and top-down micropatterning of solid surfaces to create multiscale origami structures as interface for cells (MOSAIC). In a proof-of-principle, we use this technology to analyze the activation of epidermal growth factor (EGF) receptors in living MCF7 cells using DNA origami structures decorated on their surface with distinctive nanoscale arrangements of EGF ligand entities. MOSAIC holds the potential to present to adhered cells well-defined arrangements of ligands with full control over their number, stoichiometry, and precise nanoscale orientation. It therefore promises novel applications in the life sciences, which cannot be tackled by conventional technologies.
authors.affiliation: Karlsruhe Institute of Technology (KIT), Institute for Biological Interfaces (IBG 1), Hermann-von-Helmholtz-Platz, 76344 Eggenstein-Leopoldshafen (Germany).
authors.forename: Alessandro
authors.initials: A
authors.lastname: Angelin
authors.affiliation: Karlsruhe Institute of Technology (KIT), Institute for Biological Interfaces (IBG 1), Hermann-von-Helmholtz-Platz, 76344 Eggenstein-Leopoldshafen (Germany).
authors.forename: Simone
authors.initials: S
authors.lastname: Weigel
authors.affiliation: Karlsruhe Institute of Technology (KIT), Institute for Biological Interfaces (IBG 1), Hermann-von-Helmholtz-Platz, 76344 Eggenstein-Leopoldshafen (Germany).
authors.forename: Ruben
authors.initials: R
authors.lastname: Garrecht
authors.affiliation: Karlsruhe Institute of Technology (KIT), Institute for Biological Interfaces (IBG 1), Hermann-von-Helmholtz-Platz, 76344 Eggenstein-Leopoldshafen (Germany).
authors.forename: Rebecca
authors.initials: R
authors.lastname: Meyer
authors.affiliation: Karlsruhe Institute of Technology (KIT), Institute for Biological Interfaces (IBG 1), Hermann-von-Helmholtz-Platz, 76344 Eggenstein-Leopoldshafen (Germany).
authors.forename: Jens
authors.initials: J
authors.lastname: Bauer
authors.affiliation: Karlsruhe Institute of Technology (KIT), Institute for Nanotechnology (INT) & Karlsruhe Nano Micro Facility (KNMF), Hermann-von-Helmholtz-Platz, 76344 Eggenstein-Leopoldshafen (Germany).
authors.forename: Ravi Kapoor
authors.initials: RK
authors.lastname: Kumar
authors.affiliation: Karlsruhe Institute of Technology (KIT), Institute for Nanotechnology (INT) & Karlsruhe Nano Micro Facility (KNMF), Hermann-von-Helmholtz-Platz, 76344 Eggenstein-Leopoldshafen (Germany).
authors.forename: Michael
authors.initials: M
authors.lastname: Hirtz
authors.affiliation: Karlsruhe Institute of Technology (KIT), Institute for Biological Interfaces (IBG 1), Hermann-von-Helmholtz-Platz, 76344 Eggenstein-Leopoldshafen (Germany). niemeyer@kit.edu.
authors.forename: Christof M
authors.initials: CM
authors.lastname: Niemeyer
mesh_terms: D045744:Cell Line, Tumor; D004247:DNA / Q000737:chemistry*; D006801:Humans
chemical_list: D004247:DNA
keywords: DNA; cell cultures; nanostructures; proteins; surface chemistry