title: IL-25 Receptor Expression on Airway Dendritic Cells after Allergen Challenge in Subjects with Asthma.
abstract: IL-25 is an epithelial-derived cytokine, whose effects are mediated by the IL-25 receptor (IL-17RB), and that has been implicated in the pathogenesis of allergic disease and airway viral responses. Airway myeloid dendritic cells (mDCs) and plasmacytoid dendritic cells (pDCs) are professional antigen-presenting cells. pDCs may play a protective role in asthma and are key players in the innate immune response through recognition of microbial products via Toll-like receptors (TLRs). The effects of inhaled allergens on the expression of IL-17RB by mDCs and pDCs, and the effects of IL-25 on pDCs, are unknown.
  
  
  
  To evaluate allergen-induced changes in IL-17RB expression by mDCs and pDCs and to investigate the effects of IL-25 on pDCs.
  
  
  
  Patients with mild atopic asthma (n = 13) were challenged with inhaled allergen. Blood and sputum DCs were enumerated and IL-17RB expression was determined by flow cytometry before and 7 and 24 hours after allergen challenge. The effects of IL-25 on pDCs in vitro were also assessed.
  
  
  
  Inhaled allergen significantly increased mDC and pDC numbers in sputum but not in blood. The percentage of IL-17RB(+) mDCs and pDCs was significantly increased in blood and sputum 24 hours after challenge. IL-25 up-regulated TLR9 expression by pDCs and orchestrated the responses to TLR9 ligation.
  
  
  
  IL-17RB is up-regulated on blood and sputum mDCs and pDCs after allergen inhalation. IL-25 modulates pDC function through an effect on TLR9 expression.
authors.affiliation: 1 Firestone Institute of Respiratory Health and the Department of Medicine, Michael G. DeGroote School of Medicine, McMaster University, Hamilton, Ontario, Canada; and.|2 Department of Internal Medicine, Asthma and Allergy, Medical University of Lodz, Lodz, Poland.
authors.forename: Damian
authors.initials: D
authors.lastname: Tworek
authors.affiliation: 1 Firestone Institute of Respiratory Health and the Department of Medicine, Michael G. DeGroote School of Medicine, McMaster University, Hamilton, Ontario, Canada; and.
authors.forename: Steven G
authors.initials: SG
authors.lastname: Smith
authors.affiliation: 1 Firestone Institute of Respiratory Health and the Department of Medicine, Michael G. DeGroote School of Medicine, McMaster University, Hamilton, Ontario, Canada; and.
authors.forename: Brittany M
authors.initials: BM
authors.lastname: Salter
authors.affiliation: 1 Firestone Institute of Respiratory Health and the Department of Medicine, Michael G. DeGroote School of Medicine, McMaster University, Hamilton, Ontario, Canada; and.
authors.forename: Adrian J
authors.initials: AJ
authors.lastname: Baatjes
authors.affiliation: 1 Firestone Institute of Respiratory Health and the Department of Medicine, Michael G. DeGroote School of Medicine, McMaster University, Hamilton, Ontario, Canada; and.
authors.forename: Tara
authors.initials: T
authors.lastname: Scime
authors.affiliation: 1 Firestone Institute of Respiratory Health and the Department of Medicine, Michael G. DeGroote School of Medicine, McMaster University, Hamilton, Ontario, Canada; and.
authors.forename: Rick
authors.initials: R
authors.lastname: Watson
authors.affiliation: 1 Firestone Institute of Respiratory Health and the Department of Medicine, Michael G. DeGroote School of Medicine, McMaster University, Hamilton, Ontario, Canada; and.
authors.forename: Caitlin
authors.initials: C
authors.lastname: Obminski
authors.affiliation: 1 Firestone Institute of Respiratory Health and the Department of Medicine, Michael G. DeGroote School of Medicine, McMaster University, Hamilton, Ontario, Canada; and.
authors.forename: Gail M
authors.initials: GM
authors.lastname: Gauvreau
authors.affiliation: 1 Firestone Institute of Respiratory Health and the Department of Medicine, Michael G. DeGroote School of Medicine, McMaster University, Hamilton, Ontario, Canada; and.
authors.forename: Paul M
authors.initials: PM
authors.lastname: O'Byrne
mesh_terms: D000280:Administration, Inhalation; D000293:Adolescent; D000328:Adult; D000368:Aged; D000485:Allergens / Q000008:administration & dosage / Q000276:immunology / Q000378:metabolism; D001249:Asthma / Q000276:immunology* / Q000378:metabolism; D001985:Bronchial Provocation Tests / Q000379:methods*; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D008875:Middle Aged; D018123:Receptors, Interleukin / Q000276:immunology* / Q000378:metabolism; D013183:Sputum / Q000276:immunology; D055815:Young Adult
chemical_list: D000485:Allergens; C560912:IL-25 receptor protein, human; D018123:Receptors, Interleukin
keywords: IL-17RB; TLR9; allergic asthma; dendritic cells