title: Paeoniflorin ameliorates symptoms of experimental Sjogren's syndrome associated with down-regulating Cyr61 expression.
abstract: Paeoniflorin (PF), an active compound extracted from Paeony root, has been used in therapy of autoimmune diseases with effective clinical efficiency and higher safety. Sjogren's syndrome (SS) is a chronic, systemic, immune-mediated inflammatory disease. In this study, we demonstrated that novel pro-inflammatory factor Cyr61/CCN1 was up-regulated in epithelial cells of salivary glands of primary SS patients and submandibular gland autoantigen-induced experimental SS mice. Blocking Cyr61 expression with special monoclonal antibody improved saliva secretion by ameliorating inflammatory infiltration and cytokines production in vivo. Furthermore, we showed that PF could alleviate inflammation by down-regulating Cyr61 expression in experimental SS mice. In conclusion, our new findings revealed for the first time that Cyr61 involves the pathogenesis of primary SS and PF alleviates SS-like symptoms associated with inhibiting Cyr61 expression, providing new insights into the potential molecular mechanism of PF in primary SS treatment.
authors.affiliation: Shanghai Institute of Immunology & Department of Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Huidan
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Rheumatology, the First Affiliated Hospital of Nanjing Medical University Nanjing, China.
authors.forename: Xiaoxuan
authors.initials: X
authors.lastname: Sun
authors.affiliation: Shanghai Institute of Immunology & Department of Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Shanghai Institute of Immunology & Department of Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Shanghai Institute of Immunology & Department of Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Rongfen
authors.initials: R
authors.lastname: Huo
authors.affiliation: Shanghai Institute of Immunology & Department of Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine, Shanghai, China; Maternity and Child Health Hospital, Changning, Shanghai, China.
authors.forename: Haichuan
authors.initials: H
authors.lastname: Li
authors.affiliation: Shanghai Institute of Immunology & Department of Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Tianhang
authors.initials: T
authors.lastname: Zhai
authors.affiliation: Shanghai Institute of Immunology & Department of Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Baihua
authors.initials: B
authors.lastname: Shen
authors.affiliation: Department of Rheumatology, the First Affiliated Hospital of Nanjing Medical University Nanjing, China. Electronic address: miaojia_zhang@163.com.
authors.forename: Miaojia
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Shanghai Institute of Immunology & Department of Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine, Shanghai, China. Electronic address: ninglixiaoxue57@163.com.
authors.forename: Ningli
authors.initials: N
authors.lastname: Li
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage*; D055515:Cysteine-Rich Protein 61 / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D005260:Female; D005960:Glucosides / Q000008:administration & dosage*; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008875:Middle Aged; D023421:Models, Animal; D039821:Monoterpenes / Q000008:administration & dosage*; D029596:Paeonia / Q000276:immunology; D018517:Plant Roots; D012469:Salivary Glands / Q000276:immunology*; D012859:Sjogren's Syndrome / Q000188:drug therapy* / Q000276:immunology; D015854:Up-Regulation / Q000187:drug effects; D006027:Glycosides
chemical_list: D000893:Anti-Inflammatory Agents; D055515:Cysteine-Rich Protein 61; D005960:Glucosides; D039821:Monoterpenes; C015423:peoniflorin; D006027:Glycosides
keywords: Cyr61/CCN1; Epithelial cells; Paeoniflorin; Sjogren's syndrome