title: Thin-film amperometric multibiosensor for simultaneous determination of lactate and glucose in wine.
abstract: An amperometric multi-biosensor based on lactate and glucose oxidases has been developed for determination of lactate and glucose in wine. Gold thin-film amperometric electrodes were used as multi-transducers. Analytical characteristics of the multi-biosensor developed were studied. The minimum detectable concentration was 5×10(-6) mol/l for both glucose and lactate. High reproducibility and storage stability of the multi-biosensor are demonstrated in this paper. Lactate and glucose were determined in wine, and a good correlation was obtained with concentrations determined using high-performance liquid chromatography (correlation coefficient for glucose R(2)=0.998, for lactate R(2)=0.718).
authors.affiliation: Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, 150 Zabolotnogo Str., 03143 Kyiv, Ukraine. Electronic address: luda_shkotova@yahoo.com.
authors.forename: Lyudmyla V
authors.initials: LV
authors.lastname: Shkotova
authors.affiliation: Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, 150 Zabolotnogo Str., 03143 Kyiv, Ukraine; National Taras Shevchenko University of Kyiv, Educational and Scientific Centre «Institute of Biology», 2 Glushkova Str., Block 12, 03022 Kyiv, Ukraine.
authors.forename: Nataliia Y
authors.initials: NY
authors.lastname: Piechniakova
authors.affiliation: Institute of Semiconductor Physics, National Academy of Sciences of Ukraine, 41 Nauki Prosp., 03028 Kyiv, Ukraine.
authors.forename: Oleksandr L
authors.initials: OL
authors.lastname: Kukla
authors.affiliation: Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, 150 Zabolotnogo Str., 03143 Kyiv, Ukraine.
authors.forename: Sergei V
authors.initials: SV
authors.lastname: Dzyadevych
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D055664:Electrochemical Techniques; D004566:Electrodes; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000378:metabolism; D019344:Lactic Acid / Q000032:analysis*; D015203:Reproducibility of Results; D014920:Wine / Q000032:analysis*
chemical_list: D019344:Lactic Acid; D005949:Glucose Oxidase; D005947:Glucose
keywords: Amperometric multibiosensor; Glucose oxidase; Glutaral (PubChem CID: 3485); Hydrogen Peroxide (PubChem CID: 784); Lactate oxidase; Potassium phosphate (PubChem CID: 516951); Serum albumin (1–24) (PubChem CID: 16132389); Sodium phosphate (PubChem CID: 24203); Sodium sulphate (PubChem CID: 24436); Wine; d-Glucose (PubChem CID: 5793); l-Lactic acid (PubChem CID: 107689)