title: Functional polymorphisms of Monocyte Chemoattractant Protein-1 gene and Pott's disease risk.
abstract: OBJECTIVE
  Monocyte Chemoattractant Protein-1 (MCP-1/CCL2), a key player in immune-mediated responses against Mycobacterium tuberculosis, is encoded by a polymorphic gene. Functionally relevant polymorphic variations in the MCP-1 gene have been associated with both susceptibility to and protection against tuberculosis-related disorders. Here, we investigated the potential impact of some of these polymorphisms on Pott's disease risk in a patient cohort from Algeria.
  
  
  METHODS
  DNA from 132 Algerian patients with exclusive Pott's disease and 204 healthy controls, included under a case-control design, were analyzed for the MCP1 -2518A/G (rs1024611), -362G/C (rs2857656) and int1del554-567 (rs3917887) polymorphisms. PHASE software was used for haplotype reconstruction. Genetic associations were examined using chi-square tests.
  
  
  RESULTS
  We found that the rs1024611 -2518 GG, rs2857656 -362 CC and rs3917887 int1del554-567 del/del homozygous genotypes each were significantly more prevalent in patients than in controls (respective corrected p value [Pc]=0.01, 0.04 and 0.04) Haplotype distribution profile further confirmed this, as the homozygous combination of GCdel haplotype was also found with raised susceptibility to Pott's disease (Pc=0.03).
  
  
  CONCLUSIONS
  Our findings confirm and replicate the recent data from China (which dealt essentially with rs1024611 and rs2857656) and also reinforce them by providing trans-ethnic evidence and extending the genetic association to the rs3917887.
authors.affiliation: Laboratoire de Transplantation et d'Immunogénétique, Département d'Immunologie, Institut Pasteur d'Algérie, Alger 16000, Algeria; INSERM, UMRS 1160, Hôpital Saint Louis, Paris 75010, France.
authors.forename: Fethi
authors.initials: F
authors.lastname: Mecabih
authors.affiliation: Service de Rhumatologie, Etablissement Hospitalier Spécialisé de Douera, Alger 16000, Algeria.
authors.forename: Fatiha
authors.initials: F
authors.lastname: Sadouki
authors.affiliation: INSERM, UMRS 1160, Hôpital Saint Louis, Paris 75010, France.
authors.forename: Meriem
authors.initials: M
authors.lastname: Bennabi
authors.affiliation: Laboratoire de Transplantation et d'Immunogénétique, Département d'Immunologie, Institut Pasteur d'Algérie, Alger 16000, Algeria; INSERM, UMRS 1160, Hôpital Saint Louis, Paris 75010, France.
authors.forename: Sofiane
authors.initials: S
authors.lastname: Salah
authors.affiliation: INSERM, UMRS 1160, Hôpital Saint Louis, Paris 75010, France.
authors.forename: Wahid
authors.initials: W
authors.lastname: Boukouaci
authors.affiliation: Laboratoire de Transplantation et d'Immunogénétique, Département d'Immunologie, Institut Pasteur d'Algérie, Alger 16000, Algeria.
authors.forename: Habiba
authors.initials: H
authors.lastname: Amroun
authors.affiliation: Laboratoire Jean Dausset et LabEx Transplantex, Hôpital Saint Louis, Paris 75010, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Fortier
authors.affiliation: Laboratoire Jean Dausset et LabEx Transplantex, Hôpital Saint Louis, Paris 75010, France.
authors.forename: François
authors.initials: F
authors.lastname: Marzais
authors.affiliation: Laboratoire Jean Dausset et LabEx Transplantex, Hôpital Saint Louis, Paris 75010, France; Université Paris Diderot, Sorbonne Paris-Cité, Paris 75013, France.
authors.forename: Dominique
authors.initials: D
authors.lastname: Charron
authors.affiliation: Service de Rhumatologie, Etablissement Hospitalier Spécialisé de Douera, Alger 16000, Algeria.
authors.forename: Hachemi
authors.initials: H
authors.lastname: Djoudi
authors.affiliation: Laboratoire de Transplantation et d'Immunogénétique, Département d'Immunologie, Institut Pasteur d'Algérie, Alger 16000, Algeria.
authors.forename: Mohamed Cherif
authors.initials: MC
authors.lastname: Abbadi
authors.affiliation: INSERM, UMRS 1160, Hôpital Saint Louis, Paris 75010, France.
authors.forename: Rajagopal
authors.initials: R
authors.lastname: Krishnamoorthy
authors.affiliation: INSERM, UMRS 1160, Hôpital Saint Louis, Paris 75010, France; Laboratoire Jean Dausset et LabEx Transplantex, Hôpital Saint Louis, Paris 75010, France; Université Paris Diderot, Sorbonne Paris-Cité, Paris 75013, France. Electronic address: tamouza.ryad@gmail.com.
authors.forename: Ryad
authors.initials: R
authors.lastname: Tamouza
mesh_terms: D000328:Adult; D000462:Algeria / Q000453:epidemiology; D000483:Alleles; D016022:Case-Control Studies; D018932:Chemokine CCL2 / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D015810:Linkage Disequilibrium; D008297:Male; D016017:Odds Ratio; D020641:Polymorphism, Single Nucleotide; D012306:Risk; D014399:Tuberculosis, Spinal / Q000453:epidemiology* / Q000209:etiology*; D055815:Young Adult
chemical_list: D018932:Chemokine CCL2
keywords: MCP-1; Polymorphism; Pott’s disease; Spinal tuberculosis