title: Prognostic value of TROP2 in human nasopharyngeal carcinoma.
abstract: BACKGROUND
  There is increasing evidence demonstrating the role of human trophoblast cell surface antigen 2 (TROP2) in cancer development and progression. However, their prognostic value in Epstein-Barr virus (EBV) associated nasopharyngeal carcinoma (NPC) remains to be elucidated.
  
  
  METHODS
  The prognostic significances of TROP2 and Ki-67 were determined by immunohistochemistry in 58 NPC samples. TROP2 mRNA expression level and biological functions were evaluated. The presence of EBV was assessed using in situ hybridization. Analyses were conducted on the association between each of these variables as well as clinical outcome.
  
  
  RESULTS
  TROP2 was exhibited over expression in 64% of NPC samples and significantly associated with highly proliferative tumor cells (P = 0.05) and lymph node metastases (P = 0.03). Overexpression of TROP2 significantly correlated with worse overall survival (P = 0.026) and poor disease-free survival (P = 0.021). By univariate analysis, high expression of TROP2 significantly correlated with patients with distant metastases, Ki-67 and EBV infection. Multivariate analysis further revealed that TROP2 along with Ki-67 and distant metastasis are independent prognostic predictors for NPC patients.
  
  
  CONCLUSIONS
  Our findings have demonstrated that overexpression of TROP2 appears to be an independent predictor for poor clinical outcome in NPC. The strong correlation of overexpression of TROP2 with Ki-67 and distant metastases indicates a potentially therapeutic strategies targeting TROP2 for NPC patients.
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, The Second Hospital of Jilin University Changchun 130041, P. R. China.
authors.forename: Guo-Fang
authors.initials: GF
authors.lastname: Guan
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, The Second Hospital of Jilin University Changchun 130041, P. R. China.
authors.forename: De-Jun
authors.initials: DJ
authors.lastname: Zhang
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, The Second Hospital of Jilin University Changchun 130041, P. R. China.
authors.forename: Lian-Ji
authors.initials: LJ
authors.lastname: Wen
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, The Second Hospital of Jilin University Changchun 130041, P. R. China.
authors.forename: Duo-Jiao
authors.initials: DJ
authors.lastname: Yu
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, The Second Hospital of Jilin University Changchun 130041, P. R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, The Second Hospital of Jilin University Changchun 130041, P. R. China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhu
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, The Second Hospital of Jilin University Changchun 130041, P. R. China.
authors.forename: Ying-Yuan
authors.initials: YY
authors.lastname: Guo
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, Tumor Hospital of Jilin Province Changchun 130012, P. R. China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zheng
mesh_terms: D000328:Adult; D000368:Aged; D000951:Antigens, Neoplasm / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000032:analysis*; D015153:Blotting, Western; D002277:Carcinoma; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D018572:Disease-Free Survival; D020031:Epstein-Barr Virus Infections / Q000150:complications; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D019394:Ki-67 Antigen / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000401:mortality / Q000473:pathology* / Q000821:virology; D011379:Prognosis; D016016:Proportional Hazards Models; D034741:RNA, Small Interfering; D060888:Real-Time Polymerase Chain Reaction; D014162:Transfection; D055815:Young Adult
chemical_list: D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D015815:Cell Adhesion Molecules; D019394:Ki-67 Antigen; D034741:RNA, Small Interfering; C499380:TACSTD2 protein, human
keywords: EBV; Nasopharyngeal carcinoma; TROP2; clinical outcome; prognostic predictor