title: Differential effects of three echovirus strains on cell lysis and insulin secretion in beta cell derived lines.
abstract: In an earlier study, infection of human pancreatic islets with epidemic strains of echovirus (E4, E16, E30), with proven but differently ability to induce islet autoimmunity, resulted either in a severe damage (i.e., E16 and E30) or proceeded without visible changes in infected islets (i.e., E4). In this study, the ability of these strains to replicate in beta cells and the consequence of such an infection for beta cell lysis and beta cell function was studied in the pancreatic beta cell lines INS-1, MIN6, and NIT-1. The strains of E16 and E30 did replicate in INS1, MIN6, and NIT1 cells and resulted in a pronounced cytopathic effect within 3 days following infection. By contrast, E4 replicated in all examined insulinoma cells with no apparent cell destruction. The insulin release in response to high glucose stimulation was hampered in all infected cells (P < 0.05) when no evidence of cytolysis was present; however, the adverse effect of E16 and E30 on insulin secretion appeared to be higher than that of the E4 strain. The differential effects of echovirus infection on cell lysis, and beta cell function in the rodent insulinoma INS1, MIN6, and NIT 1 cells reflect those previously obtained in primary human islets and support the notion that the insulin-producing beta cells can harbor a non-cytopathic viral infection.
authors.affiliation: Cellular Autoimmunity Unit, Department of Clinical Sciences, Skåne University Hospital, Lund University, Malmo, Sweden.
authors.forename: Luis
authors.initials: L
authors.lastname: Sarmiento
authors.affiliation: Unit of Diabetes and Celiac Disease, Department of Clinical Sciences, Skåne University Hospital, Lund University, Malmo, Sweden.
authors.forename: Anya
authors.initials: A
authors.lastname: Medina
authors.affiliation: Cellular Autoimmunity Unit, Department of Clinical Sciences, Skåne University Hospital, Lund University, Malmo, Sweden.
authors.forename: Kosrat
authors.initials: K
authors.lastname: Aziz
authors.affiliation: Department of Immunology, Genetics, and Pathology, Uppsala University, Rudbeck laboratory, Uppsala, Sweden.
authors.forename: Mahesh
authors.initials: M
authors.lastname: Anagandula
authors.affiliation: Department of Immunology and Genetics on Diabetes, National Institute of Endocrinology, Havana, Cuba.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Cabrera-Rode
authors.affiliation: Unit of Diabetes and Celiac Disease, Department of Clinical Sciences, Skåne University Hospital, Lund University, Malmo, Sweden.
authors.forename: Malin
authors.initials: M
authors.lastname: Fex
authors.affiliation: Department of Immunology, Genetics, and Pathology, Uppsala University, Rudbeck laboratory, Uppsala, Sweden.
authors.forename: Gun
authors.initials: G
authors.lastname: Frisk
authors.affiliation: Cellular Autoimmunity Unit, Department of Clinical Sciences, Skåne University Hospital, Lund University, Malmo, Sweden.
authors.forename: Corrado M
authors.initials: CM
authors.lastname: Cilio
mesh_terms: D016923:Cell Death; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D029822:Enterovirus B, Human / Q000472:pathogenicity / Q000502:physiology*; D005947:Glucose / Q000494:pharmacology; D006801:Humans; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D050417:Insulin-Secreting Cells / Q000378:metabolism* / Q000473:pathology / Q000821:virology*; D007340:Insulinoma; D014779:Virus Replication
chemical_list: D007328:Insulin; D005947:Glucose
keywords: INS 1; MIN 6; NIT 1; enterovirus; type 1 diabetes