title: Role of S180L polymorphism in etiology of malaria caused by Plasmodium falciparum in a small group of Pakistani population.
abstract: The aim of our study was to investigate the role of S180L polymorphism in modulation of acquisition of malaria caused by Plasmodium falciparum in a small group of Pakistani population. A total of 133 individuals including 60 controls and 73 patients of malaria, caused by Plasmodium falciparum, were genotyped using allele-specific PCR. Ninety-two samples successfully demonstrated the PCR amplification results, while forty-one samples could not be genotyped due to failure in PCR amplification. The allele frequency for S180L polymorphism was deviant from Hardy-Weinberg equilibrium (HWE) of the population under observation. Association was found between the observed polymorphism and the occurrence of malaria caused by Plasmodium falciparum (p = 0.01). Chances of malaria caused by Plasmodium falciparum were low in CC genotype carriers in comparison to other genotypes (Odds ratio: 0.3016; 95% CI: 0.124-0.729). The present findings suggest that S180L polymorphism is important in modulating the probability of acquisition of malaria caused by Plasmodium falciparum in Pakistani population. The CC genotype plays a protective role in local population against this type of malaria.
authors.affiliation: Department of Zoology, University of Sargodha, Sargodha- Pakistan. kashifnawazshabbir@yahoo.com.
authors.forename: Syed Kashif
authors.initials: SK
authors.lastname: Nawaz
authors.forename: Bisma
authors.initials: B
authors.lastname: Ahmed
authors.forename: Najma
authors.initials: N
authors.lastname: Arshad
authors.forename: Asima
authors.initials: A
authors.lastname: Rani
authors.forename: Hamadia
authors.initials: H
authors.lastname: Rasool
authors.forename: Muhammad
authors.initials: M
authors.lastname: Arshad
mesh_terms: D000328:Adult; D000368:Aged; D000483:Alleles; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006579:Heterozygote; D006801:Humans; D016778:Malaria, Falciparum / Q000453:epidemiology* / Q000235:genetics*; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics*; D010154:Pakistan / Q000453:epidemiology; D010963:Plasmodium falciparum; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D017472:Receptors, Interleukin-1 / Q000235:genetics*; D055815:Young Adult
chemical_list: D008562:Membrane Glycoproteins; D017472:Receptors, Interleukin-1; C434984:TIRAP protein, human