title: Bioactive Sheath/Core nanofibers containing olive leaf extract.
abstract: This study aimed at producing silk fibroin (SF)/hyaluronic acid (HA) and olive leaf extract (OLE) nanofibers with sheath/core morphology by coaxial electrospinning method, determining their antimicrobial properties, and examining release profiles of OLE from these coaxial nanofibers. Optimum electrospinning process and solution parameters were determined to obtain uniform and bead-free coaxial nanofibers. Scanning electron microscopy and transmission electron microscopy (TEM) were used to characterize the morphology of the nanofibers. The antimicrobial activities of nanofibers were tested according to AATCC test method 100. Total phenolic content and total antioxidant activity were tested using in vitro batch release system. The quality and quantity of released components of OLE were determined by high-performance liquid chromatography. The changes in nanofibers were examined by Fourier-transform infrared spectroscopy. Uniform and bead-free nanofibers were produced successfully. TEM images confirmed the coaxial structure. OLE-loaded nanofibers demonstrated almost perfect antibacterial activities against both of gram-negative and gram-positive bacteria. Antifungal activity against C. albicans was rather poor. After a release period of 1 month, it was observed that ∼70-95% of the OLE was released from nanofibers and it was still bioactive. Overall results indicate that the resultant shell/core nanofibers have a great potential to be used as biomaterials.
authors.affiliation: Department of Materials Science and Nanotechnology Engineering, Uşak University, Uşak, 64200, Turkey.
authors.forename: Gamze
authors.initials: G
authors.lastname: Doğan
authors.affiliation: Department of Textile Engineering, Ege University, İzmir, 35100, Turkey.
authors.forename: Güldemet
authors.initials: G
authors.lastname: Başal
authors.affiliation: Department of Chemical Engineering, Ege University, İzmir, 35100, Turkey.
authors.forename: Oğuz
authors.initials: O
authors.lastname: Bayraktar
authors.affiliation: Department of Biology, Ege University, İzmir, 35100, Turkey.
authors.forename: Figen
authors.initials: F
authors.lastname: Özyildiz
authors.affiliation: Department of Biology, Ege University, İzmir, 35100, Turkey.
authors.forename: Ataç
authors.initials: A
authors.lastname: Uzel
authors.affiliation: Department of Bioengineering, İzmir Institute of Technology, İzmir, 35430, Turkey.
authors.forename: İpek
authors.initials: İ
authors.lastname: Erdoğan
mesh_terms: D057139:Nanofibers / Q000737:chemistry*; D031658:Olea / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*
chemical_list: D010936:Plant Extracts
keywords: active agent release profile; antimicrobial activity; antioxidant activity; coaxial electrospinning