title: Deep sequencing identifies deregulation of microRNAs involved with vincristine drug-resistance of colon cancer cells.
abstract: BACKGROUND
  Vincristine (VCR) is a chemical that is widely used in tumor therapy. While long-term use can make tumor cells resistant to VCR, the underlying mechanisms of this resistance are still unclear.
  
  
  OBJECTIVE
  This study aimed at investigating the role of microRNA (miRNA) in colon cancer drug resistance.
  
  
  METHODS
  HCT-8 colon carcinoma cells were cultured and treated with different VCR concentrations to establish an HCT-8/VCR resistant cell line. Whole-genome screens, HiSeq 2500 sequencing, and bioinformatics methods were used to detect and analyze differences in miRNA expression between the drug-resistant HCT-8/VCR cells and non-resistant HCT-8 cells. Differential expression profiles of miRNAs were constructed based on sequencing result.
  
  
  RESULTS
  The HCT-8/VCR resistant colon carcinoma cell line was established. With regard to the difference in drug resistance between HCT-8/VCR and HCT-8 cells, 24 miRNAs showed statistically significant differences in their expression (fold change > 4), of which 17 were up-regulated. Seven miRNAs were down-regulated.
  
  
  CONCLUSIONS
  As abnormal expression of miRNAs was associated with VCR resistance of colon carcinoma cells, differences in miRNA expression may play a key role in VCR resistance of colon cancer cells.
authors.affiliation: Department of Biochemistry and Molecular Biology, Xinxiang Medical University Henan 453003 China ; Henan Collaborative Innovation Canter of Molecular Diagnosis and Laboratory Medicine Xinxiang, 453003, Henan, China.
authors.forename: Wei-Hua
authors.initials: WH
authors.lastname: Dong
authors.affiliation: Department of Biochemistry and Molecular Biology, Xinxiang Medical University Henan 453003 China ; Henan Collaborative Innovation Canter of Molecular Diagnosis and Laboratory Medicine Xinxiang, 453003, Henan, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Li
authors.affiliation: Department of Biochemistry and Molecular Biology, Xinxiang Medical University Henan 453003 China ; Henan Collaborative Innovation Canter of Molecular Diagnosis and Laboratory Medicine Xinxiang, 453003, Henan, China.
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Zhang
authors.affiliation: Department of Biochemistry and Molecular Biology, Xinxiang Medical University Henan 453003 China ; Henan Collaborative Innovation Canter of Molecular Diagnosis and Laboratory Medicine Xinxiang, 453003, Henan, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Guo
authors.affiliation: Department of Biochemistry and Molecular Biology, Xinxiang Medical University Henan 453003 China ; Henan Collaborative Innovation Canter of Molecular Diagnosis and Laboratory Medicine Xinxiang, 453003, Henan, China.
authors.forename: Huizheng
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Biochemistry and Molecular Biology, Xinxiang Medical University Henan 453003 China ; Henan Collaborative Innovation Canter of Molecular Diagnosis and Laboratory Medicine Xinxiang, 453003, Henan, China.
authors.forename: Tian-Yun
authors.initials: TY
authors.lastname: Wang
mesh_terms: D002277:Carcinoma / Q000188:drug therapy*; D045744:Cell Line, Tumor; D003106:Colon / Q000378:metabolism; D003110:Colonic Neoplasms / Q000188:drug therapy*; D019295:Computational Biology; D018076:DNA, Complementary / Q000235:genetics; D015536:Down-Regulation; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D015854:Up-Regulation; D014750:Vincristine / Q000627:therapeutic use*
chemical_list: D018076:DNA, Complementary; D035683:MicroRNAs; D014750:Vincristine
keywords: Colon cancer; HCT-8; drug resistance; microRNA