title: Cloning Endangered Felids by Interspecies Somatic Cell Nuclear Transfer.
abstract: In 2003, the first wild felid was produced by interspecies somatic cell nuclear transfer. Since then other wild felid clone offspring have been produced by using the same technique with minor modifications. This chapter describes detailed protocols used in our laboratory for (1) the isolation, culture, and preparation of fibroblast cells as donor nucleus, and (2) embryo reconstruction with domestic cat enucleated oocytes to produce cloned embryos that develop to the blastocyst stage in vitro and, after transfer into synchronized recipients, establish successful pregnancies.
authors.affiliation: Audubon Nature Center for Research of Endangered Species, 14001 River Road, New Orleans, LA, 70131, USA. martha@mcgomez.com.
authors.forename: Martha C
authors.initials: MC
authors.lastname: Gómez
authors.affiliation: Audubon Nature Center for Research of Endangered Species, 14001 River Road, New Orleans, LA, 70131, USA.
authors.forename: C Earle
authors.initials: CE
authors.lastname: Pope
mesh_terms: D000818:Animals; D002415:Cats; D018929:Cell Culture Techniques; D002459:Cell Fusion / Q000379:methods; D019976:Cloning, Organism / Q000379:methods*; D004624:Embryo Transfer; D056727:Endangered Species; D005347:Fibroblasts / Q000166:cytology; D008845:Microinjections / Q000379:methods; D053652:Nuclear Transfer Techniques; D009865:Oocytes / Q000166:cytology; D061251:Primary Cell Culture
keywords: Cloning; Interspecies somatic cell nuclear transfer; Wild felids