title: [Immunoproteomics of non water-soluble allergens from 4 legumes flours: peanut, soybean, sesame and lentil].
abstract: Peanut, soybean, sesame and lentil are members of legumes worldwide consumed by human that can induce food allergy in genetically predisposed individuals. Several protein allergens, mainly water-soluble, have been described. We studied the non water-soluble fraction from these 4 food sources using immunoproteomics tools and techniques. Flour extracts were solubilized in detergent and chaotropes and analysed in 1 and 2 dimensional gel electrophoresis (2D). Results showed numerous proteins exhibiting wide ranges of isoelectric points and relative molecular masses. When IgE immunoreactivities of 18 food allergy patients were individually tested in 1 and 2D western-blots, a very diversified IgE repertoire was observed, reflecting extensive cross-reactivities but also co-sensitizations. Besides already well known and characterized allergens, mass spectrometry analysis allowed the identification of 22 allergens undescribed until now: 10 in peanut, 2 in soybean, 3 in sesame and 7 in lentil. Three allergens are legume storage proteins and the others belong to transport proteins, nucleotide binding proteins and proteins involved in the regulation of metabolism. Seven proteins are potentially similar to allergens described in plants and fungi and 11 are not related to any known allergen. Our results contribute to increase the repertoire of legume allergens that may improve the diagnosis, categorize patients and thus provide a better treatment of patients.
authors.affiliation: Département de biologie, Faculté des sciences, Université Badji Mokhtar, Annaba, Algérie.
authors.forename: Hayette
authors.initials: H
authors.lastname: Bouakkadia
authors.affiliation: Département de biochimie, Faculté des sciences, Université Badji Mokhtar, Annaba, Algérie.
authors.forename: Aissa
authors.initials: A
authors.lastname: Boutebba
authors.affiliation: ESPCI, SMBP, USR CNRS 3149, Paris, France.
authors.forename: Iman
authors.initials: I
authors.lastname: Haddad
authors.affiliation: ESPCI, SMBP, USR CNRS 3149, Paris, France.
authors.forename: Joëlle
authors.initials: J
authors.lastname: Vinh
authors.affiliation: Biomnis, Laboratoire d'analyse de biologie médicale, Lyon, France.
authors.forename: Laurence
authors.initials: L
authors.lastname: Guilloux
authors.affiliation: Laboratoire de biochimie, Groupe "Allergie & Environnement", Hôpital Armand Trousseau, Paris, France.
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Sutra
authors.affiliation: Laboratoire de biochimie, Groupe "Allergie & Environnement", Hôpital Armand Trousseau, Paris, France.
authors.forename: Hélène
authors.initials: H
authors.lastname: Sénéchal
authors.affiliation: Laboratoire de biochimie, Groupe "Allergie & Environnement", Hôpital Armand Trousseau, Paris, France, Département infections et épidémiologie, Institut Pasteur, Paris, France.
authors.forename: Pascal
authors.initials: P
authors.lastname: Poncet
mesh_terms: D000485:Allergens / Q000302:isolation & purification* / Q000378:metabolism; D010367:Arachis / Q000276:immunology / Q000378:metabolism*; D005433:Flour; D005512:Food Hypersensitivity / Q000276:immunology / Q000378:metabolism; D006801:Humans; D027806:Lens Plant / Q000276:immunology / Q000378:metabolism*; D010940:Plant Proteins / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism; D020543:Proteome / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism; D040901:Proteomics / Q000379:methods; D031685:Sesamum / Q000276:immunology / Q000378:metabolism*; D012995:Solubility; D013025:Glycine max / Q000276:immunology / Q000378:metabolism*; D014867:Water / Q000737:chemistry
chemical_list: D000485:Allergens; D010940:Plant Proteins; D020543:Proteome; D014867:Water
keywords: 1 and 2D electrophoresis; allergens; food legumes; immunoproteomics; mass spectrometry; western blot