title: Multilocus analysis indicates that Trypanosoma cruzi I genetic substructure associated with sylvatic and domestic cycles is not an attribute conserved throughout Colombia.
abstract: Trypanosoma cruzi, the causative agent of Chagas disease, has been classified into six discrete typing units (DTUs) named TcI to TcVI. Furthermore, subcontinental scale studies based on analysis of the splice leader intergenic region (SL-IR) of the mini-exon gene have subdivided TcI in five genetic groups (Ia-Ie) related to the domestic and non-domestic cycles. However, a current review of this marker among all the sequences deposited in the GenBank demonstrates no correlation between the genetic structure and the eco-epidemiological features of parasite transmission. In this study, we performed a multilocus analysis of TcI isolates from a diverse array of hosts and vectors in a wide eco-geographical area of Colombia. Sequences from SL-IR and mitochondrial cyt b genes as well as PCR-RFLP profiles for four nuclear genes were analyzed. Multilocus analysis indicates that genetic structuration associated with sylvatic and domestic cycles in Colombia is not an attribute conserved across the entire eco-geography where TcI can be found.
authors.affiliation: Grupo de Biología y Control de Enfermedades Infecciosas, BCEI, Universidad de Antioquia, Medellín, Colombia.
authors.forename: Andrés
authors.initials: A
authors.lastname: Gómez-Palacio
authors.affiliation: Grupo de Genética Molecular, GENMOL, Universidad de Antioquia, Medellín, Colombia.
authors.forename: Juan
authors.initials: J
authors.lastname: Lopera
authors.affiliation: Grupo de Genética Molecular, GENMOL, Universidad de Antioquia, Medellín, Colombia.
authors.forename: Winston
authors.initials: W
authors.lastname: Rojas
authors.affiliation: Grupo de Genética Molecular, GENMOL, Universidad de Antioquia, Medellín, Colombia.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Bedoya
authors.affiliation: Grupo de Biología y Control de Enfermedades Infecciosas, BCEI, Universidad de Antioquia, Medellín, Colombia.
authors.forename: Omar
authors.initials: O
authors.lastname: Cantillo-Barraza
authors.affiliation: Grupo de Biología y Control de Enfermedades Infecciosas, BCEI, Universidad de Antioquia, Medellín, Colombia.
authors.forename: Johana
authors.initials: J
authors.lastname: Marín-Suarez
authors.affiliation: Grupo de Biología y Control de Enfermedades Infecciosas, BCEI, Universidad de Antioquia, Medellín, Colombia.
authors.forename: Omar
authors.initials: O
authors.lastname: Triana-Chávez
authors.affiliation: Grupo de Biología y Control de Enfermedades Infecciosas, BCEI, Universidad de Antioquia, Medellín, Colombia. Electronic address: anamejia25@gmail.com.
authors.forename: Ana
authors.initials: A
authors.lastname: Mejía-Jaramillo
mesh_terms: D014355:Chagas Disease / Q000453:epidemiology* / Q000469:parasitology*; D003105:Colombia / Q000453:epidemiology; D045303:Cytochromes b / Q000235:genetics; D021901:DNA, Intergenic; D016054:DNA, Protozoan; D014644:Genetic Variation; D006239:Haplotypes; D058885:Multilocus Sequence Typing; D010802:Phylogeny; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA; D014349:Trypanosoma cruzi / Q000145:classification* / Q000235:genetics*
chemical_list: D021901:DNA, Intergenic; D016054:DNA, Protozoan; D045303:Cytochromes b
keywords: Colombia; Trypanosoma cruzi; genetic population; multilocus analysis