title: Genetic variability of DNA repair mechanisms influences treatment outcome of gastric cancer.
abstract: The aim of the current study was to investigate the role of polymorphisms in DNA repair pathways on the clinical outcome of gastric cancer patients treated with platinum-based chemotherapy. A total of 380 gastric cancer patients treated with platinum-based chemotherapy were included in the present study. The genotypes ofERCC1rs11615 (Asn118Asn) and rs3212986 (*197G>T),ERCC2rs1799793 (Asn312Asp) and rs13181 (Lys751Gln),NBNrs1805794 (Gln185Gln) and rs1063054 (*1209A>C),RAD51rs1801321 (-61G>T) and rs12593359 (*502T>G), andXRCC3rs861539 (Thr241Met) were determined by polymerase chain reaction-restriction fragment length polymorphism, according to the manufacturer's instructions. The TC+CC genotypes ofERCC1rs11615 and GA+AA genotypes ofERCC2rs1799793 were found to be associated with improved response to chemotherapy, with an adjusted odds ratio of 1.66 (95% CI, 1.07-2.56) and 1.61 (95% CI, 1.05-2.49), respectively. Based on the results of Cox analysis, patients with TC+CC genotypes ofERCC1rs11615 and GA+AA genotypes ofERCC2rs1799793 exhibited a significantly decreased risk of mortality, with hazard ratios of 1.71 (95% CI, 1.06-2.72) and 1.97 (95% CI, 1.28-3.03), respectively. In conclusion, these results suggest thatERCC1rs11615 andERCC2rs1799793 in the DNA repair pathways may be used as predictive factors of the clinical outcome in gastric cancer patients.
authors.affiliation: Department of Radiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China.
authors.forename: Changmao
authors.initials: C
authors.lastname: Ding
authors.affiliation: Department of Radiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China.
authors.forename: Huiyu
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Pathology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China.
authors.forename: Kuisheng
authors.initials: K
authors.lastname: Chen
authors.affiliation: Department of General Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China.
authors.forename: Chunlin
authors.initials: C
authors.lastname: Zhao
authors.affiliation: Department of Radiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China.
authors.forename: Jianbo
authors.initials: J
authors.lastname: Gao
keywords: DNA repair pathway; clinical outcome; gastric cancer; single nucleotide polymorphism