title: Seed Dormancy in Arabidopsis Is Controlled by Alternative Polyadenylation of DOG1.
abstract: DOG1 (Delay of Germination 1) is a key regulator of seed dormancy in Arabidopsis (Arabidopsis thaliana) and other plants. Interestingly, the C terminus of DOG1 is either absent or not conserved in many plant species. Here, we show that in Arabidopsis, DOG1 transcript is subject to alternative polyadenylation. In line with this, mutants in RNA 3' processing complex display weakened seed dormancy in parallel with defects in DOG1 proximal polyadenylation site selection, suggesting that the short DOG1 transcript is functional. This is corroborated by the finding that the proximally polyadenylated short DOG1 mRNA is translated in vivo and complements the dog1 mutant. In summary, our findings indicate that the short DOG1 protein isoform produced from the proximally polyadenylated DOG1 mRNA is a key player in the establishment of seed dormancy in Arabidopsis and characterizes a set of mutants in RNA 3' processing complex required for production of proximally polyadenylated functional DOG1 transcript.
authors.affiliation: Institute of Biochemistry and Biophysics, Department of Protein Biosynthesis, Pawinskiego 5a, 02-106 Warsaw, Poland (M.C., H.F., A.C., Y.G., A.S., L.B., K.K., Z.P., S.K., S.S.); Warsaw University, Department of Chemistry, Pasteura 1, 02-093 Warsaw, Poland (A.C.); andQueen's University Belfast, School of Biological Sciences, Belfast BT9 7BL, Northern Ireland (F.L.).
authors.forename: Malgorzata
authors.identifier: http://orcid.org/0000-0001-7449-8081
authors.initials: M
authors.lastname: Cyrek
authors.affiliation: Institute of Biochemistry and Biophysics, Department of Protein Biosynthesis, Pawinskiego 5a, 02-106 Warsaw, Poland (M.C., H.F., A.C., Y.G., A.S., L.B., K.K., Z.P., S.K., S.S.); Warsaw University, Department of Chemistry, Pasteura 1, 02-093 Warsaw, Poland (A.C.); andQueen's University Belfast, School of Biological Sciences, Belfast BT9 7BL, Northern Ireland (F.L.).
authors.forename: Halina
authors.initials: H
authors.lastname: Fedak
authors.affiliation: Institute of Biochemistry and Biophysics, Department of Protein Biosynthesis, Pawinskiego 5a, 02-106 Warsaw, Poland (M.C., H.F., A.C., Y.G., A.S., L.B., K.K., Z.P., S.K., S.S.); Warsaw University, Department of Chemistry, Pasteura 1, 02-093 Warsaw, Poland (A.C.); andQueen's University Belfast, School of Biological Sciences, Belfast BT9 7BL, Northern Ireland (F.L.).
authors.forename: Arkadiusz
authors.initials: A
authors.lastname: Ciesielski
authors.affiliation: Institute of Biochemistry and Biophysics, Department of Protein Biosynthesis, Pawinskiego 5a, 02-106 Warsaw, Poland (M.C., H.F., A.C., Y.G., A.S., L.B., K.K., Z.P., S.K., S.S.); Warsaw University, Department of Chemistry, Pasteura 1, 02-093 Warsaw, Poland (A.C.); andQueen's University Belfast, School of Biological Sciences, Belfast BT9 7BL, Northern Ireland (F.L.).
authors.forename: Yanwu
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Institute of Biochemistry and Biophysics, Department of Protein Biosynthesis, Pawinskiego 5a, 02-106 Warsaw, Poland (M.C., H.F., A.C., Y.G., A.S., L.B., K.K., Z.P., S.K., S.S.); Warsaw University, Department of Chemistry, Pasteura 1, 02-093 Warsaw, Poland (A.C.); andQueen's University Belfast, School of Biological Sciences, Belfast BT9 7BL, Northern Ireland (F.L.).
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Sliwa
authors.affiliation: Institute of Biochemistry and Biophysics, Department of Protein Biosynthesis, Pawinskiego 5a, 02-106 Warsaw, Poland (M.C., H.F., A.C., Y.G., A.S., L.B., K.K., Z.P., S.K., S.S.); Warsaw University, Department of Chemistry, Pasteura 1, 02-093 Warsaw, Poland (A.C.); andQueen's University Belfast, School of Biological Sciences, Belfast BT9 7BL, Northern Ireland (F.L.).
authors.forename: Lien
authors.initials: L
authors.lastname: Brzezniak
authors.affiliation: Institute of Biochemistry and Biophysics, Department of Protein Biosynthesis, Pawinskiego 5a, 02-106 Warsaw, Poland (M.C., H.F., A.C., Y.G., A.S., L.B., K.K., Z.P., S.K., S.S.); Warsaw University, Department of Chemistry, Pasteura 1, 02-093 Warsaw, Poland (A.C.); andQueen's University Belfast, School of Biological Sciences, Belfast BT9 7BL, Northern Ireland (F.L.).
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Krzyczmonik
authors.affiliation: Institute of Biochemistry and Biophysics, Department of Protein Biosynthesis, Pawinskiego 5a, 02-106 Warsaw, Poland (M.C., H.F., A.C., Y.G., A.S., L.B., K.K., Z.P., S.K., S.S.); Warsaw University, Department of Chemistry, Pasteura 1, 02-093 Warsaw, Poland (A.C.); andQueen's University Belfast, School of Biological Sciences, Belfast BT9 7BL, Northern Ireland (F.L.).
authors.forename: Zbigniew
authors.initials: Z
authors.lastname: Pietras
authors.affiliation: Institute of Biochemistry and Biophysics, Department of Protein Biosynthesis, Pawinskiego 5a, 02-106 Warsaw, Poland (M.C., H.F., A.C., Y.G., A.S., L.B., K.K., Z.P., S.K., S.S.); Warsaw University, Department of Chemistry, Pasteura 1, 02-093 Warsaw, Poland (A.C.); andQueen's University Belfast, School of Biological Sciences, Belfast BT9 7BL, Northern Ireland (F.L.).
authors.forename: Szymon
authors.initials: S
authors.lastname: Kaczanowski
authors.affiliation: Institute of Biochemistry and Biophysics, Department of Protein Biosynthesis, Pawinskiego 5a, 02-106 Warsaw, Poland (M.C., H.F., A.C., Y.G., A.S., L.B., K.K., Z.P., S.K., S.S.); Warsaw University, Department of Chemistry, Pasteura 1, 02-093 Warsaw, Poland (A.C.); andQueen's University Belfast, School of Biological Sciences, Belfast BT9 7BL, Northern Ireland (F.L.).
authors.forename: Fuquan
authors.identifier: http://orcid.org/0000-0001-8764-6370
authors.initials: F
authors.lastname: Liu
authors.affiliation: Institute of Biochemistry and Biophysics, Department of Protein Biosynthesis, Pawinskiego 5a, 02-106 Warsaw, Poland (M.C., H.F., A.C., Y.G., A.S., L.B., K.K., Z.P., S.K., S.S.); Warsaw University, Department of Chemistry, Pasteura 1, 02-093 Warsaw, Poland (A.C.); andQueen's University Belfast, School of Biological Sciences, Belfast BT9 7BL, Northern Ireland (F.L.) sswiez@ibb.waw.pl.
authors.forename: Szymon
authors.initials: S
authors.lastname: Swiezewski
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics* / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D017124:Conserved Sequence; D018506:Gene Expression Regulation, Plant; D018525:Germination; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D010641:Phenotype; D057445:Plant Dormancy / Q000235:genetics*; D026723:Polyadenylation / Q000235:genetics*; D014176:Protein Biosynthesis; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D012323:RNA Processing, Post-Transcriptional; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012639:Seeds / Q000235:genetics / Q000502:physiology*
chemical_list: D029681:Arabidopsis Proteins; C532384:DOG1 protein, Arabidopsis; D020033:Protein Isoforms; D012333:RNA, Messenger