title: The importance of start codon of nosM in nosiheptide production.
abstract: The present study was designed to investigate the effects of start codon of nosM on the biosynthesis of nosiheptide. Target genes were amplified by overlap PCR. After homologous recombination to construct engineered strains, nosiheptide production was analyzed by HPLC. Three mutants with different start codon of nosM were constructed, and nosiheptide production of each mutant was analyzed and compared. Replacement of the start codon of nosM significantly decreased the production of nosiheptide. In conclusion, start codon usage could greatly affect the biosynthetic efficiency in the biosynthetic gene cluster of nosiheptide.
authors.affiliation: State Key Laboratory of Natural Medicines and Laboratory of Chemical Biology, China Pharmaceutical University, Nanjing 210009, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Jiang
authors.affiliation: State Key Laboratory of Natural Medicines and Laboratory of Chemical Biology, China Pharmaceutical University, Nanjing 210009, China.
authors.forename: Yan-Jiu
authors.initials: YJ
authors.lastname: Xue
authors.affiliation: State Key Laboratory of Natural Medicines and Laboratory of Chemical Biology, China Pharmaceutical University, Nanjing 210009, China.
authors.forename: Wei-Ying
authors.initials: WY
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Natural Medicines and Laboratory of Chemical Biology, China Pharmaceutical University, Nanjing 210009, China.
authors.forename: Min
authors.initials: M
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Natural Medicines and Laboratory of Chemical Biology, China Pharmaceutical University, Nanjing 210009, China.
authors.forename: Xu-Ri
authors.initials: XR
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Natural Medicines and Laboratory of Chemical Biology, China Pharmaceutical University, Nanjing 210009, China. Electronic address: shuzhenwang@cpu.edu.cn.
authors.forename: Shu-Zhen
authors.initials: SZ
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Natural Medicines and Laboratory of Chemical Biology, China Pharmaceutical University, Nanjing 210009, China. Electronic address: yjchen@cpu.edu.cn.
authors.forename: Yi-Jun
authors.initials: YJ
authors.lastname: Chen
mesh_terms: D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D002851:Chromatography, High Pressure Liquid; D018387:Codon, Initiator; D004926:Escherichia coli; D005798:Genes, Bacterial; D009154:Mutation; D013302:Streptomyces / Q000235:genetics / Q000378:metabolism; D013844:Thiazoles / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D018387:Codon, Initiator; D013844:Thiazoles; C014134:nosiheptide
keywords: Biosynthesi; Leader peptide; Nosiheptide; Start codon