title: Stress tolerance and biocontrol performance of the yeast antagonist, Candida diversa, change with morphology transition.
abstract: As an eco-friendly management method, biological control of postharvest diseases, utilizing antagonistic yeasts, is a research topic receiving considerable attention. Detailed knowledge on the biology of yeast antagonists is crucial when considering their potential application and development as biocontrol products. Changes in the growth form, such as single-cell to pseudohyphae, have been associated with the mode of action in postharvest biocontrol yeasts. In this study, the antagonistic yeast, Candida diversa, reversibly shifted from a single-cell morphology on yeast peptone dextrose (YPD) medium with 2 % agar to a pseudohyphal morphology on YPD with 0.3 % agar. The tolerance of the pseudohyphal form to heat and oxidative stresses, as well as the biocontrol efficacy against Botrytis cinerea on apple and kiwifruit stored at 25 and 4 °C, was significantly higher as compared to the single-cell form. This study provides new information on the ability of C. diversa to change its morphology and the impact of the morphology shift on stress tolerance and biocontrol performance.
authors.affiliation: School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei, 230009, China.
authors.forename: Guangkun
authors.initials: G
authors.lastname: Li
authors.affiliation: School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei, 230009, China.
authors.forename: Mengshan
authors.initials: M
authors.lastname: Chi
authors.affiliation: School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei, 230009, China.
authors.forename: Huizhen
authors.initials: H
authors.lastname: Chen
authors.affiliation: School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei, 230009, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Sui
authors.affiliation: Key Laboratory of Plant Pathology, Ministry of Agriculture, Department of Plant Pathology, China Agricultural University, Beijing, 100193, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei, 230009, China.|Anhui Wanjiang Institute of Poultry Technology, Hefei University of Technology, Xuancheng Campus, Xuancheng, 242000, China.
authors.forename: Yongsheng
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Yantai Lvyun Biotechnology Co. Ltd, Yantai, 264003, China.
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Yantai Lvyun Biotechnology Co. Ltd, Yantai, 264003, China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Sun
authors.affiliation: School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei, 230009, China.|Anhui Wanjiang Institute of Poultry Technology, Hefei University of Technology, Xuancheng Campus, Xuancheng, 242000, China.
authors.forename: Guoqing
authors.initials: G
authors.lastname: Liu
authors.affiliation: Key Laboratory of Plant Pathology, Ministry of Agriculture, Department of Plant Pathology, China Agricultural University, Beijing, 100193, China. wangqi@cau.edu.cn.
authors.forename: Qi
authors.initials: Q
authors.lastname: Wang
authors.affiliation: School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei, 230009, China. jialiu1983@163.com.|Anhui Wanjiang Institute of Poultry Technology, Hefei University of Technology, Xuancheng Campus, Xuancheng, 242000, China. jialiu1983@163.com.
authors.forename: Jia
authors.initials: J
authors.lastname: Liu
mesh_terms: D029042:Actinidia / Q000382:microbiology; D000362:Agar / Q000378:metabolism; D061046:Biological Control Agents / Q000494:pharmacology*; D020171:Botrytis / Q000502:physiology*; D002175:Candida / Q000166:cytology* / Q000254:growth & development / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D006358:Hot Temperature; D027845:Malus / Q000382:microbiology; D018384:Oxidative Stress; D010935:Plant Diseases / Q000382:microbiology / Q000517:prevention & control*; D013312:Stress, Physiological / Q000187:drug effects*
chemical_list: D061046:Biological Control Agents; D000362:Agar
keywords: Eco-friendly management; Morphology transition; Postharvest disease; Stress tolerance; Yeast biocontrol agent