title: miR-874 suppresses the proliferation and metastasis of osteosarcoma by targeting E2F3.
abstract: Increasing evidence indicates that microRNAs (miRNAs) play critical roles in osteosarcoma (OS) occurrence and development. MicroRNA-874 (miR-874) has proven to be dysregulated in several human cancers. However, the biological function and underlying molecular mechanism of miR-874 in OS remain unclear. In this study, we aimed to investigate the biological role and potential mechanism of miR-874 in OS. Here, we found that miR-874 expression was significantly decreased in OS cell lines and tissues by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR), and its expression was correlated with tumor-node-metastasis (TNM) stage, tumor size, and lymph node metastasis (all P < 0.01). Functional study revealed that overexpression of miR-874 in OS cells could remarkably inhibit proliferation, migration, and invasion and induce cell apoptosis. In addition, E2F transcription factor 3 (E2F3) was confirmed as a target of miR-874 in OS cells. E2F3 mRNA expression was upregulated and was inversely correlated with the level of miR-874 in OS tissues. Importantly, downregulation of E2F3 mimicked the effect of overexpression miR-874 in OS cells, and E2F3 overexpression partially attenuated the tumor-suppressive effects of miR-874 in OS cells. Taken together, these findings suggested that miR-874 might suppress the growth and metastasis of OS cells partially by targeting E2F3.
authors.affiliation: Department of Radiology, The First Hospital of Jilin University, No.71 Xinmin Street, Changchun, 130021, Jilin, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Dong
authors.affiliation: Department of Joint Surgery, The First Hospital of Jilin University, No. 71 Xinmin Street, Changchun, 130021, Jilin, China.
authors.forename: Yubao
authors.initials: Y
authors.lastname: Gong
authors.affiliation: Department of Joint Surgery, The First Hospital of Jilin University, No. 71 Xinmin Street, Changchun, 130021, Jilin, China.
authors.forename: Debao
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Department of Joint surgery, Inner Monggolia People's Hospital, Huhehaote, 010000, Inner Monggolia, China.
authors.forename: Huricha
authors.initials: H
authors.lastname: Bao
authors.affiliation: Department of Joint Surgery, The First Hospital of Jilin University, No. 71 Xinmin Street, Changchun, 130021, Jilin, China. guguisan1110@126.com.
authors.forename: Guishan
authors.initials: G
authors.lastname: Gu
mesh_terms: D020413:3' Untranslated Regions; D000328:Adult; D000368:Aged; D017209:Apoptosis / Q000235:genetics; D001665:Binding Sites; D001859:Bone Neoplasms / Q000235:genetics* / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D015536:Down-Regulation; D050699:E2F3 Transcription Factor / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging; D012516:Osteosarcoma / Q000235:genetics* / Q000473:pathology*; D034622:RNA Interference; D047368:Tumor Burden
chemical_list: D020413:3' Untranslated Regions; D050699:E2F3 Transcription Factor; C561946:MIRN874 microRNA, human; D035683:MicroRNAs
keywords: E2F3; Osteosarcoma; miR-874; microRNAs