title: IL-1β Is Involved with the Generation of Pain in Experimental Autoimmune Encephalomyelitis.
abstract: Pain is one of the main symptoms of multiple sclerosis, a demyelinating disease of the central nervous system that affects millions of people worldwide. The experimental autoimmune encephalomyelitis (EAE) is considered an experimental model of multiple sclerosis, and besides motor weakness, hypernociception is one of the clinical signs of animals with EAE. In this study, we investigated the influence of some cytokines in the generation of the hypernociceptive response in a mouse model of EAE using MOG35-55. We measured some cytokines in the dorsal root ganglia (DRG), an important anatomical structure involved in pain. We found increased levels of the cytokines TNF-α, IL-1β, and Kc in DRGs of animals with EAE. We used the antibody IL-1ra to antagonize the effects of IL-1β, and animals presented a decrease in the hypernociceptive response. Thus, our results suggest that hypernociception in this experimental model of EAE may be a consequence of the increase in some cytokines in DRGs, especially IL-1β.
authors.affiliation: Department of Basic and Health Sciences, Universidade Federal de Juiz de Fora-campus Governador Valadares, de Juiz de Fora, Brazil. dhenrodrigues@gmail.com.|Translational Psychoneuroimmunology Group, Laboratory of Immunopharmacology, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil. dhenrodrigues@gmail.com.|Department of Biochemistry, Universidade Federal de Minas Gerais, Belo Horizonte, MG, 31270-901, Brazil. dhenrodrigues@gmail.com.
authors.forename: David Henrique
authors.initials: DH
authors.lastname: Rodrigues
authors.affiliation: Department of Biochemistry, Universidade Federal de Minas Gerais, Belo Horizonte, MG, 31270-901, Brazil.
authors.forename: Bruno Pereira
authors.initials: BP
authors.lastname: Leles
authors.affiliation: Department of Biochemistry, Universidade Federal de Minas Gerais, Belo Horizonte, MG, 31270-901, Brazil.
authors.forename: Vivian Vasconcelos
authors.initials: VV
authors.lastname: Costa
authors.affiliation: Translational Psychoneuroimmunology Group, Laboratory of Immunopharmacology, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
authors.forename: Aline Silva
authors.initials: AS
authors.lastname: Miranda
authors.affiliation: Department of Biochemistry, Universidade Federal de Minas Gerais, Belo Horizonte, MG, 31270-901, Brazil.
authors.forename: Daniel
authors.initials: D
authors.lastname: Cisalpino
authors.affiliation: Department of Biochemistry, Universidade Federal de Minas Gerais, Belo Horizonte, MG, 31270-901, Brazil.
authors.forename: Dawidson Assis
authors.initials: DA
authors.lastname: Gomes
authors.affiliation: Department of Biochemistry, Universidade Federal de Minas Gerais, Belo Horizonte, MG, 31270-901, Brazil.
authors.forename: Danielle Glória
authors.initials: DG
authors.lastname: de Souza
authors.affiliation: Translational Psychoneuroimmunology Group, Laboratory of Immunopharmacology, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.|Department of Biochemistry, Universidade Federal de Minas Gerais, Belo Horizonte, MG, 31270-901, Brazil.
authors.forename: Antônio Lúcio
authors.initials: AL
authors.lastname: Teixeira
mesh_terms: D000818:Animals; D054360:Chemokine CXCL1 / Q000378:metabolism; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000150:complications* / Q000235:genetics / Q000378:metabolism* / Q000503:physiopathology; D005260:Female; D005727:Ganglia, Spinal / Q000378:metabolism / Q000473:pathology; D053590:Interleukin 1 Receptor Antagonist Protein / Q000378:metabolism; D053583:Interleukin-1beta / Q000378:metabolism*; D008810:Mice, Inbred C57BL; D063308:Myelin-Oligodendrocyte Glycoprotein; D062559:NAV1.8 Voltage-Gated Sodium Channel / Q000235:genetics / Q000378:metabolism; D059225:Nociception; D010146:Pain / Q000150:complications* / Q000235:genetics / Q000378:metabolism* / Q000503:physiopathology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013116:Spinal Cord / Q000378:metabolism / Q000473:pathology; D050916:TRPV Cation Channels / Q000235:genetics / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D054360:Chemokine CXCL1; C475895:Cxcl1 protein, mouse; D053590:Interleukin 1 Receptor Antagonist Protein; D053583:Interleukin-1beta; D063308:Myelin-Oligodendrocyte Glycoprotein; D062559:NAV1.8 Voltage-Gated Sodium Channel; D012333:RNA, Messenger; C568318:Scn10a protein, mouse; D050916:TRPV Cation Channels; C493921:TRPV1 protein, mouse; D014409:Tumor Necrosis Factor-alpha
keywords: Cytokines; Dorsal root ganglia; Experimental autoimmune encephalomyelitis; Hypernociception