title: Keratins 17 and 19 expression as prognostic markers in oral squamous cell carcinoma.
abstract: Five-year survival rates for oral squamous cell carcinoma (OSCC) are 30% and the mortality rate is 50%. Immunohistochemistry panels are used to evaluate proliferation, vascularization, apoptosis, HPV infection, and keratin expression, which are important markers of malignant progression. Keratins are a family of intermediate filaments predominantly expressed in epithelial cells and have an essential role in mechanical support and cytoskeleton formation, which is essential for the structural integrity and stability of the cell. In this study, we analyzed the expressions of keratins 17 and 19 (K17 and K19) by immunohistochemistry in tumoral and non-tumoral tissues from patients with OSCC. The results show that expression of these keratins is higher in tumor tissues compared to non-tumor tissues. Positive K17 expression correlates with lymph node metastasis and multivariate analysis confirmed this relationship, revealing a 6-fold increase in lymph node metastasis when K17 is expressed. We observed a correlation between K17 expression with disease-free survival and disease-specific death in patients who received surgery and radiotherapy. Multivariate analysis revealed that low expression of K17 was an independent marker for early disease relapse and disease-specific death in patients treated with surgery and radiotherapy, with an approximately 4-fold increased risk when compared to high K17 expression. Our results suggest a potential role for K17 and K19 expression profiles as tumor prognostic markers in OSCC patients.
authors.affiliation: Laboratório de Biologia Molecular, Hospital Heliópolis, São Paulo, SP, Brasil.
authors.forename: B A
authors.initials: BA
authors.lastname: Coelho
authors.affiliation: Programa de Pós-Graduação em Biotecnologia, Universidade Federal do Espírito Santo, Vitória, ES, Brasil.
authors.forename: G T
authors.initials: GT
authors.lastname: Peterle
authors.affiliation: Escola Multicampi de Ciências Médicas do Rio Grande do Norte, Universidade Federal do Rio Grande do Norte, Caicó, RN, Brasil.
authors.forename: M
authors.initials: M
authors.lastname: Santos
authors.affiliation: Programa de Pós-Graduação em Biotecnologia, Universidade Federal do Espírito Santo, Vitória, ES, Brasil.
authors.forename: L P
authors.initials: LP
authors.lastname: Agostini
authors.affiliation: Programa de Pós-Graduação em Biotecnologia, Universidade Federal do Espírito Santo, Vitória, ES, Brasil.
authors.forename: L L
authors.initials: LL
authors.lastname: Maia
authors.affiliation: Programa de Pós-Graduação em Biotecnologia, Universidade Federal do Espírito Santo, Vitória, ES, Brasil.
authors.forename: E
authors.initials: E
authors.lastname: Stur
authors.affiliation: Programa de Pós-Graduação em Biotecnologia, Universidade Federal do Espírito Santo, Vitória, ES, Brasil.
authors.forename: C V M
authors.initials: CV
authors.lastname: Silva
authors.affiliation: Programa de Pós-Graduação em Biotecnologia, Universidade Federal do Espírito Santo, Vitória, ES, Brasil.
authors.forename: S O
authors.initials: SO
authors.lastname: Mendes
authors.affiliation: Programa de Pós-Graduação em Biotecnologia, Universidade Federal do Espírito Santo, Vitória, ES, Brasil.
authors.forename: C C J
authors.initials: CC
authors.lastname: Almança
authors.affiliation: Programa de Pós-Graduação em Biotecnologia, Universidade Federal do Espírito Santo, Vitória, ES, Brasil.
authors.forename: F V
authors.initials: FV
authors.lastname: Freitas
authors.affiliation: Programa de Pós-Graduação em Biotecnologia, Universidade Federal do Espírito Santo, Vitória, ES, Brasil.
authors.forename: A R
authors.initials: AR
authors.lastname: Borçoi
authors.affiliation: Programa de Pós-Graduação em Biotecnologia, Universidade Federal do Espírito Santo, Vitória, ES, Brasil.
authors.forename: A B
authors.initials: AB
authors.lastname: Archanjo
authors.affiliation: Laboratório de Biologia Molecular, Hospital Heliópolis, São Paulo, SP, Brasil.
authors.forename: A M C
authors.initials: AM
authors.lastname: Mercante
authors.affiliation: Departamento de Patologia Bucal, Faculdade de Odontologia, Universidade de São Paulo, São Paulo, SP, Brasil.
authors.forename: F D
authors.initials: FD
authors.lastname: Nunes
authors.affiliation: Laboratório de Biologia Molecular, Hospital Heliópolis, São Paulo, SP, Brasil.
authors.forename: M B
authors.initials: MB
authors.lastname: Carvalho
authors.affiliation: Departamento de Biologia Molecular, Faculdade de Medicina, São José do Rio Preto, SP, Brasil.
authors.forename: E H
authors.initials: EH
authors.lastname: Tajara
authors.affiliation: Programa de Pós-Graduação em Biotecnologia, Universidade Federal do Espírito Santo, Vitória, ES, Brasil.
authors.forename: I D
authors.initials: ID
authors.lastname: Louro
authors.affiliation: Programa de Pós-Graduação em Biotecnologia, Universidade Federal do Espírito Santo, Vitória, ES, Brasil.
authors.forename: A M A
authors.initials: AM
authors.lastname: Silva-Conforti
mesh_terms: D014408:Biomarkers, Tumor / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology / Q000628:therapy; D018572:Disease-Free Survival; D004847:Epithelial Cells / Q000378:metabolism; D005260:Female; D006801:Humans; D007633:Keratins / Q000378:metabolism*; D008207:Lymphatic Metastasis / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000378:metabolism* / Q000473:pathology / Q000628:therapy; D009364:Neoplasm Recurrence, Local / Q000378:metabolism / Q000473:pathology; D011379:Prognosis
chemical_list: D014408:Biomarkers, Tumor; D007633:Keratins