title: Healing of localized gingival recessions treated with a coronally advanced flap alone or combined with an enamel matrix derivative and a porcine acellular dermal matrix: a preclinical study.
abstract: OBJECTIVE
  This study aimed to evaluate the effects of a porcine acellular dermal matrix (PADM) with or without an enamel matrix derivative (EMD) on gingival recession defects treated with a coronally advanced flap (CAF) in dogs.
  
  
  METHODS
  Miller class II gingival recession defects (5 mm wide and 7 mm deep) were surgically created on the labial side of bilateral maxillary canines in 12 dogs. After 8 weeks of plaque accumulation, the 24 chronic defects were randomly assigned to one of the following 4 treatments: CAF, CAF with PADM (CAF/PADM), CAF with EMD (CAF/EMD), and CAF with EMD and PADM (CAF/EMD/PADM). The animals were sacrificed 10 weeks after surgery for histologic evaluation.
  
  
  RESULTS
  In all groups, root coverage was obtained to a varying degree. PADM was well incorporated in gingival connective tissue in the CAF/PADM and in the CAF/EMD/PADM groups. The height of newly formed bone was significantly greater in the CAF/EMD/PADM group than in the CAF and CAF/PADM groups. New cementum with periodontal ligament-like tissue was predominantly found in the CAF/EMD and CAF/EMD/PADM groups. The CAF/EMD/PADM group showed the greatest amount of new cementum among the groups examined, although the difference was not statistically significant.
  
  
  CONCLUSIONS
  Within the limitations of the present study, it can be concluded that CAF/EMD/PADM treatment may promote periodontal regeneration in gingival recession defects.
  
  
  CONCLUSIONS
  The present results suggest that the combination of EMD and PADM in conjunction with CAF may represent a promising approach for treating single Miller class II gingival recessions.
authors.affiliation: Department of Periodontology, Kagoshima University Graduate School of Medical and Dental Sciences, 8-35-1, Sakuragaoka, Kagoshima, 890-8544, Japan. syoshi@dent.kagoshima-u.ac.jp.
authors.forename: Y
authors.initials: Y
authors.lastname: Shirakata
authors.affiliation: Department of Periodontology, School of Dental Medicine, University of Bern, Bern, Switzerland.
authors.forename: A
authors.initials: A
authors.lastname: Sculean
authors.affiliation: Department of Periodontology, Kagoshima University Graduate School of Medical and Dental Sciences, 8-35-1, Sakuragaoka, Kagoshima, 890-8544, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Shinohara
authors.affiliation: Department of Periodontology, Kagoshima University Graduate School of Medical and Dental Sciences, 8-35-1, Sakuragaoka, Kagoshima, 890-8544, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Sena
authors.affiliation: Department of Periodontology, Kagoshima University Graduate School of Medical and Dental Sciences, 8-35-1, Sakuragaoka, Kagoshima, 890-8544, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Takeuchi
authors.affiliation: Department of Periodontology, School of Dental Medicine, University of Bern, Bern, Switzerland.|Department of Oral Surgery and Stomatology, School of Dental Medicine, University of Bern, Bern, Switzerland.
authors.forename: D D
authors.initials: DD
authors.lastname: Bosshardt
authors.affiliation: Department of Periodontology, Kagoshima University Graduate School of Medical and Dental Sciences, 8-35-1, Sakuragaoka, Kagoshima, 890-8544, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Noguchi
mesh_terms: D062088:Acellular Dermis; D000818:Animals; D003131:Combined Modality Therapy; D003746:Dental Enamel Proteins / Q000494:pharmacology*; D004285:Dogs; D005889:Gingival Recession / Q000188:drug therapy* / Q000601:surgery*; D005893:Gingivoplasty / Q000379:methods; D012038:Regeneration; D013524:Surgical Flaps; D013552:Swine; D014945:Wound Healing / Q000502:physiology*
chemical_list: D003746:Dental Enamel Proteins
keywords: Animal study; Enamel matrix derivative; Gingival recession defects; Periodontal regeneration; Porcine acellular dermal matrix; Root coverage