title: Coumarins and flavonoid from Murraya paniculata (L.) Jack: Antibacterial and anti-inflammation activity.
abstract: The ethyl acetate extract of leaves of Murraya paniculata (L.) Jack was described in the previous in vitro study on the inhibition effect on the growth of periodontopathic bacteria and the reduction of cytokines from LPS-stimulated macrophages. In this study, four coumarins including murrangatin (1), murrangatin acetate (2), murranganonesenecionate (3), micropubescin (4) and one flavonoid, 3', 4', 5', 7-tetramethoxyflavone (5) were isolated from the leaves of ethyl acetate extract of M. paniculata. MTT assay was used to test cytotoxicity on human gingival fibroblast and monocytes. The isolated compounds were evaluated for their antibacterial effect against Porphyromonas gingivalis (ATCC33277) and anti-inflammation on lipopolysaccharide-stimulated inflammation using monocyte cells. All isolated compounds exhibited antibacterial activity against P. gingivalis (ATCC 33277). Murranganonesenecionate (3) was highly potent anti-inflammation properties. The coumarin constituents from M. paniculata leaves might be potential lead molecules for the development of antimicrobial drugs for treating periodontal disease.
authors.affiliation: Department of Advanced General Dentistry, Faculty of Dentistry, Mahidol University, Bangkok, Thailand.
authors.forename: Pirasut
authors.initials: P
authors.lastname: Rodanant
authors.affiliation: Department of Chemistry, Faculty of Science, Ramkhamhaeng University, Bangkok, Thailand.
authors.forename: Pichit
authors.initials: P
authors.lastname: Khetkam
authors.affiliation: Department of Chemistry, Faculty of Science, Ramkhamhaeng University, Bangkok, Thailand.
authors.forename: Apichart
authors.initials: A
authors.lastname: Suksamrarn
authors.affiliation: Department of Microbiology, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand.
authors.forename: Jintakorn
authors.initials: J
authors.lastname: Kuvatanasuchati
mesh_terms: D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D002470:Cell Survival / Q000187:drug effects; D003374:Coumarins / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D006801:Humans; D007249:Inflammation / Q000276:immunology / Q000378:metabolism / Q000517:prevention & control*; D020128:Inhibitory Concentration 50; D009000:Monocytes / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D008517:Phytotherapy; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D018515:Plant Leaves; D010946:Plants, Medicinal; D016966:Porphyromonas gingivalis / Q000187:drug effects* / Q000254:growth & development; D027821:Rutaceae / Q000737:chemistry; D020298:U937 Cells
chemical_list: D000900:Anti-Bacterial Agents; D000893:Anti-Inflammatory Agents; D003374:Coumarins; D005419:Flavonoids; D010936:Plant Extracts