title: Disruption and eradication of P. aeruginosa biofilms using nitric oxide-releasing chitosan oligosaccharides.
abstract: Biofilm disruption and eradication were investigated as a function of nitric oxide- (NO) releasing chitosan oligosaccharide dose and the results compared with control (i.e., non-NO-releasing) chitosan oligosaccharides and tobramycin. Quantification of biofilm expansion/contraction and multiple-particle tracking microrheology were used to assess the structural integrity of the biofilm before and after antibacterial treatment. While tobramycin had no effect on the physical properties of the biofilm, NO-releasing chitosan oligosaccharides exhibited dose-dependent behavior with biofilm degradation. Control chitosan oligosaccharides increased biofilm elasticity, indicating that the scaffold may mitigate the biofilm disrupting power of nitric oxide somewhat. The results from this study indicate that nitric oxide-releasing chitosan oligosaccharides act as dual-action therapeutics capable of eradicating and physically disrupting P. aeruginosa biofilms.
authors.affiliation: a Department of Chemistry , University of North Carolina at Chapel Hill , Chapel Hill , NC , USA.
authors.forename: Katelyn P
authors.initials: KP
authors.lastname: Reighard
authors.affiliation: b The Marsico Lung Institute , University of North Carolina at Chapel Hill , Chapel Hill , NC , USA.|c Department of Physics and Astronomy , University of North Carolina at Chapel Hill , Chapel Hill , NC , USA.
authors.forename: David B
authors.initials: DB
authors.lastname: Hill
authors.affiliation: b The Marsico Lung Institute , University of North Carolina at Chapel Hill , Chapel Hill , NC , USA.
authors.forename: Graham A
authors.initials: GA
authors.lastname: Dixon
authors.affiliation: a Department of Chemistry , University of North Carolina at Chapel Hill , Chapel Hill , NC , USA.
authors.forename: Brittany V
authors.initials: BV
authors.lastname: Worley
authors.affiliation: a Department of Chemistry , University of North Carolina at Chapel Hill , Chapel Hill , NC , USA.
authors.forename: Mark H
authors.initials: MH
authors.lastname: Schoenfisch
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D048271:Chitosan / Q000138:chemical synthesis / Q000737:chemistry*; D009569:Nitric Oxide / Q000737:chemistry / Q000494:pharmacology*; D009844:Oligosaccharides / Q000138:chemical synthesis / Q000737:chemistry*; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000254:growth & development; D012212:Rheology; D014031:Tobramycin / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D009844:Oligosaccharides; D009569:Nitric Oxide; D048271:Chitosan; D014031:Tobramycin
keywords: Nitric oxide; P. aeruginosa; biofilm; rheology