title: Marek's disease virus undergoes complete morphogenesis after reactivation in a T-lymphoblastoid cell line transformed by recombinant fluorescent marker virus.
abstract: T-lymphocytes are central targets of Marek's disease, a major chicken disease induced by the oncogenic alphaherpesvirus Marek's disease virus (MDV). T-lymphocyte infection is also associated with immunosuppression and virus latency. To decipher viral morphogenesis in T-lymphocytes, we used the recombinant vRB-1B 47EGFP marker virus to generate a new lymphoblastoid cell line, 3867K, that exhibited typical properties of other MDV-transformed chicken cell lines in term of cell markers, reactivation rate and infectivity. Examination of reactivating EGFP-positive 3867K cells by transmission electron microscopy revealed the presence of most types of herpesvirus particles inside the cells but no extracellular ones. Quantification of virion types indicated only 5% cytoplasmic particles, with 0.5% being mature. This study demonstrated that MDV morphogenesis is complete upon reactivation in T-lymphocytes, albeit with poor efficiency, with a defect in the exit of virions from the nucleus and secondary envelopment, as occurs in infected fibroblasts.
authors.affiliation: INRA UMR1282 Infectious Disease and Public Health, ISP, BIOVA team, 37380 Nouzilly, France.
authors.forename: Caroline
authors.initials: C
authors.lastname: Denesvre
authors.affiliation: INRA UMR1282 Infectious Disease and Public Health, ISP, BIOVA team, 37380 Nouzilly, France.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Rémy
authors.affiliation: INRA UMR1282 Infectious Disease and Public Health, ISP, BIOVA team, 37380 Nouzilly, France.
authors.forename: Laetitia
authors.initials: L
authors.lastname: Trapp-Fragnet
authors.affiliation: Avian Oncogenic Virus Group, The Pirbright Institute, Guildford, GU24 0NF, UK.
authors.forename: Lorraine P
authors.initials: LP
authors.lastname: Smith
authors.affiliation: Département des Microscopies (Plateau Technologique Analyse des Systèmes Biologiques), Université François Rabelais, Tours, France.
authors.forename: Sonia
authors.initials: S
authors.lastname: Georgeault
authors.affiliation: INRA UMR1282 Infectious Disease and Public Health, ISP, BIOVA team, 37380 Nouzilly, France.
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Vautherot
authors.affiliation: Avian Oncogenic Virus Group, The Pirbright Institute, Guildford, GU24 0NF, UK.
authors.forename: Venugopal
authors.initials: V
authors.lastname: Nair
mesh_terms: D000818:Animals; D002460:Cell Line; D002645:Chickens; D005347:Fibroblasts / Q000821:virology; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000235:genetics; D008381:Herpesvirus 2, Gallid / Q000235:genetics / Q000502:physiology*; D046529:Microscopy, Electron, Transmission; D008967:Molecular Biology / Q000379:methods; D013601:T-Lymphocytes / Q000821:virology*; D014771:Virion / Q000648:ultrastructure*; D014773:Virology / Q000379:methods; D014775:Virus Activation; D019065:Virus Assembly
chemical_list: C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins