title: Analysis of Recombinant Proteins in Transgenic Rice Seeds: Identity, Localization, Tolerance to Digestion, and Plant Stress Response.
abstract: Rice seeds are an ideal production platform for high-value recombinant proteins in terms of economy, scalability, safety, and stability. Strategies for the expression of large amounts of recombinant proteins in rice seeds have been established in the past decade and transgenic rice seeds that accumulate recombinant products such as bioactive peptides and proteins, which promote the health and quality of life of humans, have been generated in many laboratories worldwide. One of the most important advantages is the potential for direct oral delivery of transgenic rice seeds without the need for recombinant protein purification (downstream processing), which has been attributed to the high expression levels of recombinant products. Transgenic rice will be beneficial as a delivery system for pharmaceuticals and nutraceuticals in the future. This chapter introduces the strategy for producing recombinant protein in the edible part (endosperm) of the rice grain and describes methods for the analysis of transgenic rice seeds in detail.
authors.affiliation: Functional Transgenic Crops Research Unit, Genetically Modified Organism Research Center, National Institute of Agrobiological Sciences, Kannondai 2-1-2, Tsukuba, Ibaraki, 305-8602, Japan.
authors.forename: Yuhya
authors.initials: Y
authors.lastname: Wakasa
authors.affiliation: Functional Transgenic Crops Research Unit, Genetically Modified Organism Research Center, National Institute of Agrobiological Sciences, Kannondai 2-1-2, Tsukuba, Ibaraki, 305-8602, Japan. takaiwa@affrc.go.jp.
authors.forename: Fumio
authors.initials: F
authors.lastname: Takaiwa
mesh_terms: D056625:Endosperm / Q000378:metabolism* / Q000502:physiology; D012275:Oryza / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D030821:Plants, Genetically Modified; D059748:Proteolysis; D011994:Recombinant Proteins / Q000032:analysis / Q000378:metabolism*; D013312:Stress, Physiological
chemical_list: D011994:Recombinant Proteins
keywords: Oryza sativa L.; Protein body; Recombinant protein; Seed storage protein; Transgenic rice