title: Molecular cloning and expression analysis of jasmonic acid dependent but salicylic acid independent LeWRKY1.
abstract: Various plant genes can be activated or inhibited by phytohormones under conditions of biotic and abiotic stress, especially in response to jasmonic acid (JA) and salicylic acid (SA). Interactions between JA and SA may be synergistic or antagonistic, depending on the stress condition. In this study, we cloned a full-length cDNA (LeWRKY1, GenBank accession No. FJ654265) from Lycopersicon esculentum by rapid amplification of cDNA ends. Sequence analysis showed that this gene is a group II WRKY transcription factor. Analysis of LeWRKY1 mRNA expression in various tissues by qRT-PCR showed that the highest and lowest expression occurred in the leaves and stems, respectively. In addition, LeWRKY1 expression was induced by JA and Botrytis cinerea Pers., but not by SA.
authors.affiliation: Plant Science and Technology College, Beijing University of Agriculture, Beijing, China.
authors.forename: M
authors.initials: M
authors.lastname: Lu
authors.affiliation: School of Life Sciences, Shandong Normal University, Jinan, China.
authors.forename: L F
authors.initials: LF
authors.lastname: Wang
authors.affiliation: School of Life Sciences, Shandong Normal University, Jinan, China.
authors.forename: X H
authors.initials: XH
authors.lastname: Du
authors.affiliation: Center of Tissue Culture, Beijing University of Agriculture, Beijing, China.
authors.forename: Y K
authors.initials: YK
authors.lastname: Yu
authors.affiliation: Plant Science and Technology College, Beijing University of Agriculture, Beijing, China.
authors.forename: J B
authors.initials: JB
authors.lastname: Pan
authors.affiliation: Plant Science and Technology College, Beijing University of Agriculture, Beijing, China.
authors.forename: Z J
authors.initials: ZJ
authors.lastname: Nan
authors.affiliation: Plant Science and Technology College, Beijing University of Agriculture, Beijing, China.
authors.forename: J
authors.initials: J
authors.lastname: Han
authors.affiliation: Plant Science and Technology College, Beijing University of Agriculture, Beijing, China.
authors.forename: W X
authors.initials: WX
authors.lastname: Wang
authors.affiliation: Plant Science and Technology College, Beijing University of Agriculture, Beijing, China.
authors.forename: Q Z
authors.initials: QZ
authors.lastname: Zhang
authors.affiliation: Plant Science and Technology College, Beijing University of Agriculture, Beijing, China.
authors.forename: Q P
authors.initials: QP
authors.lastname: Sun
mesh_terms: D020171:Botrytis / Q000378:metabolism; D003001:Cloning, Molecular / Q000379:methods; D003517:Cyclopentanes / Q000378:metabolism*; D018076:DNA, Complementary / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D017343:Genes, Plant / Q000235:genetics*; D018551:Solanum lycopersicum / Q000235:genetics*; D054883:Oxylipins / Q000378:metabolism*; D010937:Plant Growth Regulators / Q000235:genetics; D018515:Plant Leaves / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics*; D018547:Plant Stems / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D020156:Salicylic Acid / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics
chemical_list: D003517:Cyclopentanes; D018076:DNA, Complementary; D054883:Oxylipins; D010937:Plant Growth Regulators; D010940:Plant Proteins; D012333:RNA, Messenger; D014157:Transcription Factors; C011006:jasmonic acid; D020156:Salicylic Acid