title: Acetobacter mustisp. nov., isolated from Bobal grape must.
abstract: An acetic acid bacterium (strain Bo7T), obtained during a study of the microbial diversity of spontaneous fermentations of Bobal grape must, was subjected to a taxonomic study using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences allocated strain Bo7Tto the genusAcetobacter, and revealedAcetobacter acetiandAcetobacter oenito be nearest neighbours (99.57 % 16S rRNA gene sequence similarity between strain Bo7TandA. oeniCECT 5830T, and 98.76 % between strain Bo7TandA. acetiCECT 298T). Cells of strain Bo7Tare Gram-negative, motile rods, catalase-positive and oxidase-negative. The DNA G+C content of strain Bo7Twas 58.0 mol%. DNA-DNA hybridizations demonstrated that strain Bo7Tbelongs to a single novel genospecies that can be differentiated from its nearest phylogenetic neighbours by the following phenotypic characteristics: no production of 5-keto-d-gluconic acid from d-glucose, growth with glycerol but not with methanol or maltose as sole carbon sources, and growth on yeast extract with 30 % d-glucose. The major fatty acid was C18 : 1ω7c/C18 : 1ω6c(summed feature 8; approx. 56 %); other fatty acids in significant amounts (>5 %) were C16 : 02-OH (11 %), C16 : 0(7 %), C14 : 02-OH (7 %) and C14 : 03-OH/iso-C16 : 1I (summed feature 2; 6 %). The results obtained indicate that strain Bo7Trepresents a novel species of the genusAcetobacter, for which the nameAcetobacter mustisp. nov. is proposed. The type strain is Bo7T( = DSM 23824T= CECT 7722T).
authors.affiliation: ENOLAB - Laboratori de Microbiologia Enològica, ERI-ISIC BioTecMed, Universitat de València, c/ Dr. Moliner 50 E46100, Burjassot-València, Spain.
authors.forename: Sergi
authors.initials: S
authors.lastname: Ferrer
authors.affiliation: ENOLAB - Laboratori de Microbiologia Enològica, ERI-ISIC BioTecMed, Universitat de València, c/ Dr. Moliner 50 E46100, Burjassot-València, Spain.
authors.forename: Rosario
authors.initials: R
authors.lastname: Mañes-Lázaro
authors.affiliation: ENOLAB - Laboratori de Microbiologia Enològica, ERI-ISIC BioTecMed, Universitat de València, c/ Dr. Moliner 50 E46100, Burjassot-València, Spain.
authors.forename: Yaiza
authors.initials: Y
authors.lastname: Benavent-Gil
authors.affiliation: ENOLAB - Laboratori de Microbiologia Enològica, ERI-ISIC BioTecMed, Universitat de València, c/ Dr. Moliner 50 E46100, Burjassot-València, Spain.
authors.forename: Alba
authors.initials: A
authors.lastname: Yépez
authors.affiliation: ENOLAB - Laboratori de Microbiologia Enològica, ERI-ISIC BioTecMed, Universitat de València, c/ Dr. Moliner 50 E46100, Burjassot-València, Spain.
authors.forename: Isabel
authors.initials: I
authors.lastname: Pardo