title: Enterobacter bugandensissp. nov., isolated from neonatal blood.
abstract: A total of 17Enterobacter-like isolates were obtained from blood during a septicaemia outbreak in a neonatal unit, Tanzania, that could not be assigned based on phenotypic test to any existingEnterobacterspecies. Eight representative outbreak isolates were investigated in detail. Fermentation characteristics, biochemical assays and fatty acid profiles for taxonomic analysis were determined and supplemented with information derived from whole genome sequences. Phenotypic and morphological tests revealed that these isolates were Gram-stain-negative, rod-shaped, highly motile and facultatively anaerobic. The fatty acid profile was similar to those of the type strains for all recognizedEnterobacterspecies, with quantitative differences in C17 : 0, C18 : 1ω7cand C17 : 0cyclo fatty acids. Whole genome sequencing was used to identify taxonomically relevant characteristics, i.e. for 16S rRNA gene sequence analysis, multi-locus sequence analysis (MLSA),in silicoDNA-DNA hybridization (isDDH) and average nucleotide identity (ANI). Draft genomes were approximately 4.9 Mb in size with a G+C content of 56.0 mol%. The 16S rRNA gene sequence of these eight isolates showed >97 % similarity to allEnterobacterspecies, while MLSA clustered them closely with the type strains ofEnterobacter xiangfangensisandEnterobacter hormaechei. These eight strains showed less than 70 %isDDH identity with the type strains ofEnterobacterspecies. In addition, less than 95 % ANI to the type strains ofEnterobacterspecies was observed. From these results, it is concluded that these isolates possess sufficient characteristics to differentiate them from all recognizedEnterobacterspecies, and should therefore be considered as representing a novel species. The nameEnterobacter bugandensissp. nov. is proposed with EB-247T( = DSM 29888T= NCCB 100573T) as the type strain.
authors.affiliation: Institute for Medical Microbiology, Justus-Liebig University, and German Centre for Infection Research (DZIF), Partner Site Giessen-Marburg-Langen, Campus Giessen, Justus-Liebig University, Germany.
authors.forename: Swapnil
authors.initials: S
authors.lastname: Doijad
authors.affiliation: Institute for Medical Microbiology, Justus-Liebig University, and German Centre for Infection Research (DZIF), Partner Site Giessen-Marburg-Langen, Campus Giessen, Justus-Liebig University, Germany.
authors.forename: Can
authors.initials: C
authors.lastname: Imirzalioglu
authors.affiliation: Institute for Medical Microbiology, Justus-Liebig University, and German Centre for Infection Research (DZIF), Partner Site Giessen-Marburg-Langen, Campus Giessen, Justus-Liebig University, Germany.
authors.forename: Yancheng
authors.initials: Y
authors.lastname: Yao
authors.affiliation: Institute for Medical Microbiology, Justus-Liebig University, and German Centre for Infection Research (DZIF), Partner Site Giessen-Marburg-Langen, Campus Giessen, Justus-Liebig University, Germany.
authors.forename: Niladri Bhusan
authors.initials: NB
authors.lastname: Pati
authors.affiliation: Institute for Medical Microbiology, Justus-Liebig University, and German Centre for Infection Research (DZIF), Partner Site Giessen-Marburg-Langen, Campus Giessen, Justus-Liebig University, Germany.
authors.forename: Linda
authors.initials: L
authors.lastname: Falgenhauer
authors.affiliation: Institute for Medical Microbiology, Justus-Liebig University, and German Centre for Infection Research (DZIF), Partner Site Giessen-Marburg-Langen, Campus Giessen, Justus-Liebig University, Germany.
authors.forename: Torsten
authors.initials: T
authors.lastname: Hain
authors.affiliation: Leibniz Institute DSMZ - German Collection of Microorganisms and Cell Cultures, Braunschweig, and German Centre of Infection Research (DZIF), Partner Site Hannover-Braunschweig, Braunschweig, Germany.
authors.forename: Bärbel U
authors.initials: BU
authors.lastname: Foesel
authors.affiliation: Leibniz Institute DSMZ - German Collection of Microorganisms and Cell Cultures, Braunschweig, and German Centre of Infection Research (DZIF), Partner Site Hannover-Braunschweig, Braunschweig, Germany.
authors.forename: Birte
authors.initials: B
authors.lastname: Abt
authors.affiliation: Leibniz Institute DSMZ - German Collection of Microorganisms and Cell Cultures, Braunschweig, and German Centre of Infection Research (DZIF), Partner Site Hannover-Braunschweig, Braunschweig, Germany.
authors.forename: Jörg
authors.initials: J
authors.lastname: Overmann
authors.affiliation: Department of Microbiology, Weill Bugando School of Medicine, PO Box 1464, Mwanza, Tanzania.
authors.forename: Mariam M
authors.initials: MM
authors.lastname: Mirambo
authors.affiliation: Department of Microbiology, Weill Bugando School of Medicine, PO Box 1464, Mwanza, Tanzania.
authors.forename: Stephen E
authors.initials: SE
authors.lastname: Mshana
authors.affiliation: Institute for Medical Microbiology, Justus-Liebig University, and German Centre for Infection Research (DZIF), Partner Site Giessen-Marburg-Langen, Campus Giessen, Justus-Liebig University, Germany.
authors.forename: Trinad
authors.initials: T
authors.lastname: Chakraborty