title: 2,8-Decadiene-1,10-Diol Inhibits Lipopolysaccharide-Induced Inflammatory Responses Through Inactivation of Mitogen-Activated Protein Kinase and Nuclear Factor-κB Signaling Pathway.
abstract: Amomum tsao-ko (A. tsao-ko) has been used as a traditional medicine for the treatment of infectious and digestive disorders. In the present study, we report the anti-inflammatory activity and molecular mechanism of 2,8-decadiene-1,10-diol (DDO) isolated from the extract of A. tsao-ko in lipopolysaccharide-stimulated RAW 264.7 cells. DDO treatment inhibited the production of nitric oxide and prostaglandin E2 by downregulating inducible nitric oxide synthase and cyclooxygenase-2 expression, respectively. Moreover, DDO suppressed the production of pro-inflammatory cytokines such as interleukin-6 and tumor necrosis factor-α. These inhibitory effects of DDO on the expression of inflammatory proteins were found to be mediated through the inactivation of mitogen-activated protein kinases (MAPKs) such as extracellular signal-regulated kinase, c-Jun-N-terminal kinase and p38(MAPK), and inhibition of nuclear factor-κB (NF-κB) pathways including degradation of inhibitor of κB-α and nuclear localization of NF-κB. Taken together, these findings demonstrate the pharmacological roles and molecular mechanisms of DDO in regulating inflammatory responses, and suggest further evaluation and development of DDO as a potent therapeutic agent for the treatment of inflammatory disorders.
authors.affiliation: Department of Pharmacy, College of Pharmacy, Dankook University, Cheonan, 31116, Republic of Korea.
authors.forename: Mi-Sun
authors.initials: MS
authors.lastname: Kim
authors.affiliation: Bio-center, Gyeonggi Institute of Science & Technology Promotion, Suwon, 16229, Republic of Korea.
authors.forename: Eun-Kyung
authors.initials: EK
authors.lastname: Ahn
authors.affiliation: Bio-center, Gyeonggi Institute of Science & Technology Promotion, Suwon, 16229, Republic of Korea.
authors.forename: Seong Su
authors.initials: SS
authors.lastname: Hong
authors.affiliation: Department of Pharmacy, College of Pharmacy, Dankook University, Cheonan, 31116, Republic of Korea. jsoh@dankook.ac.kr.|Bio-center, Gyeonggi Institute of Science & Technology Promotion, Suwon, 16229, Republic of Korea. jsoh@dankook.ac.kr.
authors.forename: Joa Sub
authors.initials: JS
authors.lastname: Oh
mesh_terms: D035801:Amomum / Q000378:metabolism; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002461:Cell Line, Transformed; D051546:Cyclooxygenase 2 / Q000096:biosynthesis; D015232:Dinoprostone / Q000096:biosynthesis; D015536:Down-Regulation / Q000187:drug effects; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism*; D005233:Fatty Alcohols / Q000494:pharmacology*; D007249:Inflammation / Q000188:drug therapy* / Q000473:pathology; D015850:Interleukin-6 / Q000096:biosynthesis; D048031:JNK Mitogen-Activated Protein Kinases / Q000378:metabolism*; D008070:Lipopolysaccharides; D055670:Medicine, Korean Traditional; D051379:Mice; D000072000:NF-KappaB Inhibitor alpha / Q000378:metabolism*; D016328:NF-kappa B / Q000037:antagonists & inhibitors* / Q000378:metabolism; D009569:Nitric Oxide / Q000096:biosynthesis; D052247:Nitric Oxide Synthase Type II / Q000096:biosynthesis; D010936:Plant Extracts / Q000494:pharmacology; D000067996:RAW 264.7 Cells; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism*
chemical_list: C000613264:2,8-decadiene-1,10-diol; D000893:Anti-Inflammatory Agents; D005233:Fatty Alcohols; D015850:Interleukin-6; D008070:Lipopolysaccharides; D016328:NF-kappa B; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D000072000:NF-KappaB Inhibitor alpha; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse; C077623:Ptgs2 protein, mouse; D051546:Cyclooxygenase 2; D048049:Extracellular Signal-Regulated MAP Kinases; D048031:JNK Mitogen-Activated Protein Kinases; D048051:p38 Mitogen-Activated Protein Kinases; D015232:Dinoprostone
keywords: 2,8-decadiene-1,10-diol; mitogen-activated protein kinases; nitric oxide; nuclear factor-κB; prostaglandin E2