title: IBDV particles packaged with only segment A dsRNA.
abstract: Multi-segmented dsRNA viruses have been suggested to utilize cis-acting elements in the plus-strand RNA to accomplish genomic RNA assortment during viral packaging. It is not clear if bi-segmented dsRNA birnavirus uses the same strategy. By applying a reverse genetic technique, we generated IBDV particles packaged with only segment A by co-transfection DF-1 cells of cDNA from segment A and VP1 (without 5' and 3' noncoding region of segment B) supporting random assortment mechanism and indicating the packaging elements of segment B include sequences in the 5' and 3' NCR. However, gfp-containing IBDV could not be generated in the presence of gfp cDNA constructs flanked by 5' and 3' NCR from segment A or segment B. The data suggest additional packaging signals are required for IBDV genomic packaging. The presence of VP1 protein in the IBDV-A particles also suggests the formation of ribonucleoprotein (RNP) complexes might be involved in the assembly of viral particles.
authors.affiliation: Department of Comparative Pathobiology, College of Veterinary Medicine, Purdue University, West Lafayette, IN 47907, USA.
authors.forename: Yung-Yi C
authors.initials: YY
authors.lastname: Mosley
authors.affiliation: Department of Comparative Pathobiology, College of Veterinary Medicine, Purdue University, West Lafayette, IN 47907, USA.
authors.forename: Ching Ching
authors.initials: CC
authors.lastname: Wu
authors.affiliation: Department of Comparative Pathobiology, College of Veterinary Medicine, Purdue University, West Lafayette, IN 47907, USA. Electronic address: tllin@purdue.edu.
authors.forename: Tsang Long
authors.initials: TL
authors.lastname: Lin
mesh_terms: D000818:Animals; D002460:Cell Line; D002645:Chickens; D007243:Infectious bursal disease virus / Q000235:genetics / Q000502:physiology*; D012330:RNA, Double-Stranded / Q000235:genetics / Q000378:metabolism*; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism*; D059386:Reverse Genetics; D019065:Virus Assembly
chemical_list: D012330:RNA, Double-Stranded; D012367:RNA, Viral
keywords: Cis-acting packaging signals; IBDV; IBDV-A viral particles; Noncoding regions; Segment A