title: Microscopy-Based High-Content Screening.
abstract: Image-based screening is used to measure a variety of phenotypes in cells and whole organisms. Combined with perturbations such as RNA interference, small molecules, and mutations, such screens are a powerful method for gaining systematic insights into biological processes. Screens have been applied to study diverse processes, such as protein-localization changes, cancer cell vulnerabilities, and complex organismal phenotypes. Recently, advances in imaging and image-analysis methodologies have accelerated large-scale perturbation screens. Here, we describe the state of the art for image-based screening experiments and delineate experimental approaches and image-analysis approaches as well as discussing challenges and future directions, including leveraging CRISPR/Cas9-mediated genome engineering.
authors.affiliation: Division Signaling and Functional Genomics, German Cancer Research Center (DKFZ) and Department of Cell and Molecular Biology, Heidelberg University, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany; German Cancer Consortium (DKTK), 69120 Heidelberg, Germany. Electronic address: m.boutros@dkfz.de.
authors.forename: Michael
authors.initials: M
authors.lastname: Boutros
authors.affiliation: Division Signaling and Functional Genomics, German Cancer Research Center (DKFZ) and Department of Cell and Molecular Biology, Heidelberg University, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany.
authors.forename: Florian
authors.initials: F
authors.lastname: Heigwer
authors.affiliation: Division Signaling and Functional Genomics, German Cancer Research Center (DKFZ) and Department of Cell and Molecular Biology, Heidelberg University, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany.
authors.forename: Christina
authors.initials: C
authors.lastname: Laufer
mesh_terms: D064113:CRISPR-Cas Systems; D002477:Cells / Q000737:chemistry* / Q000166:cytology; D057166:High-Throughput Screening Assays; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008853:Microscopy; D011506:Proteins / Q000032:analysis; D034622:RNA Interference
chemical_list: D011506:Proteins