title: Simultaneous saccharification and co-fermentation for improving the xylose utilization of steam exploded corn stover at high solid loading.
abstract: Simultaneous saccharification and co-fermentation (SSCF) of steam exploded corn stover (SECS) was investigated at 5-25% solid loadings compared with other conversion processes. SECS was washed with a 15-fold excess of deionized water to remove inhibitors of hydrolysis and fermentation. The concentration, yield, and productivity of ethanol was 34.3g/L, 90.0%, 2.61g/L/h in the co-fermentation of 60g/L glucose and 10g/L xylose by Saccharomyces cerevisiae IPE003. Ethanol concentration and productivity increased with increasing solid loading while ethanol yield decreased in all conversion processes of SECS. Glucan and xylan conversion was 82.0% and 82.1% in SSCF at 20% solid loading, respectively, while the concentration, yield and productivity of ethanol was 60.8g/L, 75.3% and 0.63g/L/h. The feeding strategy of SECS addition within 24h improved the SSCF performance. Therefore, SSCF increased ethanol productivity and was an effective conversion process for ethanol production at high solid loading.
authors.affiliation: State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China; University of Chinese Academy of Sciences, Beijing 100190, China.
authors.forename: Zhi-Hua
authors.initials: ZH
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China. Electronic address: hzchen@ipe.ac.cn.
authors.forename: Hong-Zhang
authors.initials: HZ
authors.lastname: Chen
mesh_terms: D000431:Ethanol / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D006868:Hydrolysis; D012441:Saccharomyces cerevisiae / Q000254:growth & development / Q000378:metabolism; D013227:Steam; D014994:Xylose / Q000378:metabolism*; D003313:Zea mays / Q000737:chemistry*
chemical_list: D013227:Steam; D000431:Ethanol; D014994:Xylose; D005947:Glucose
keywords: Fed-batch; High solid loading; S. cerevisiae IPE003; Simultaneous saccharification and co-fermentation; Xylose fermentation