title: Separation, characterization and anticancer activities of a sulfated polysaccharide from Undaria pinnatifida.
abstract: The purpose of this paper was to investigate separation, characterization and anticancer activities of a sulfated polysaccharide (SPUP) from Undaria pinnatifida. Firstly, polysaccharide from U. pinnatifida was separated by DEAE-52 cellulose and Sephacryl S-400 column chromatography. As results, SPUP was obtained with the yield of 19.42%. Then, SPUP was characterized using chemical analysis, gas chromatography, size-exclusion HPLC chromatography, UV-vis spectra and FT-IR spectrum. The content of total sugar, uronic acid, protein and sulfate radical were 80.48%, 3.21%, 7.12% and 29.14%, respectively. SPUP was a heteropolysaccharide composed of fucose, glucose and galactose in a molar percentage of 27.15:19.34:53.51 with molecular weight of 97.9 kDa. Finally, the strongly against breast cancer activity of SPUP was confirmed by DMBA-induced breast cancer rats model. AS results, SPUP can significantly restrain breast abnormal enlargement, prolong tumor latency and reduced tumor incidence. Immunomodulatory activity and regulating abnormal sex hormones level might contribute to its anticancer activities.
authors.affiliation: School of Integrated Chinese and Western Medicine, Binzhou Medical University, Yantai 264003, Shangdong, PR China. Electronic address: hykh888@163.com.
authors.forename: Yun
authors.initials: Y
authors.lastname: Han
authors.affiliation: School of Chinese Medicine, Shandong College of Traditional Chinese Medicine, Yantai 264199, Shangdong, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wu
authors.affiliation: Affiliated Huaian Hospital of Xuzhou Medical College, Huaian 223002, Jiangsu, PR China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Liu
authors.affiliation: Affiliated Huaian Hospital of Xuzhou Medical College, Huaian 223002, Jiangsu, PR China.
authors.forename: Youdong
authors.initials: Y
authors.lastname: Hu
authors.affiliation: School of Integrated Chinese and Western Medicine, Binzhou Medical University, Yantai 264003, Shangdong, PR China.
authors.forename: Qiusheng
authors.initials: Q
authors.lastname: Zheng
authors.affiliation: School of Integrated Chinese and Western Medicine, Binzhou Medical University, Yantai 264003, Shangdong, PR China.
authors.forename: Binsheng
authors.initials: B
authors.lastname: Wang
authors.affiliation: School of Integrated Chinese and Western Medicine, Binzhou Medical University, Yantai 264003, Shangdong, PR China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Lin
authors.affiliation: Affiliated Huaian Hospital of Xuzhou Medical College, Huaian 223002, Jiangsu, PR China. Electronic address: xial_li@qq.com.
authors.forename: Xia
authors.initials: X
authors.lastname: Li
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology*; D002850:Chromatography, Gel / Q000379:methods; D005260:Female; D005643:Fucose / Q000737:chemistry; D005690:Galactose / Q000737:chemistry; D005947:Glucose / Q000737:chemistry; D008970:Molecular Weight; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013431:Sulfates / Q000737:chemistry; D044648:Undaria
chemical_list: D000970:Antineoplastic Agents; D011134:Polysaccharides; D013431:Sulfates; C069025:sulfate radical; D005643:Fucose; D005947:Glucose; D005690:Galactose
keywords: Characterization and anticancer activity; Polysaccharide; Separation; Undaria pinnatifida