title: Expression of human papillomavirus and prognosis of juvenile laryngeal papilloma.
abstract: The aim of this study was to evaluate the correlation between clinical behavior and expression of human papillomavirus (HPV) in patients with juvenile laryngeal papillomatosis, in an attempt to develop an effective molecular biological method to predict prognosis. We included 37 patients with juvenile laryngeal papillomatosis in the study group and 10 cases each of juvenile vocal cord polyps and juvenile normal laryngeal mucosa as the control group. We detected HPV by immunocytochemistry and in situ hybridization, identified the virus type, and measured HPV-DNA content using a computer-assisted, color pathological image-analysis system. Additionally, we conducted a retrospective study with regard to the patients' clinical history to evaluate the prognosis. The data of the 2 groups were compared and statistically analyzed, including a correlation with prognosis. In the study group, 67.3% (25/37) were positive for HPV-Ag by immunocytochemistry; whereas 53.2%, 45.8%, and 25.4% were positive for HPV6b-DNA, HPV11-DNA, and HPV6b+11-DNA, respectively, by in situ hybridization. HPV was not detected in the control group. There was a significant difference between two groups (P < 0.05). Compared to HPV11-DNA-positive cases, those that were positive for HPV6b-DNA and HPV6b+11-DNA showed lower results on average, for age at first diagnosis and self-relief, number of surgeries, and interval between surgeries. Our findings suggest that immunocytochemistry and in situ hybridization are useful methods to evaluate the prognosis of juvenile laryngeal papilloma (JLP) and that HPV6b-positivity can be used as an index to predict the development and outcome of JLP.
authors.affiliation: Department of Otolaryngology, Tongren Hospital Affiliated to Shanghai Jiaotong University School of Medicine Shanghai 200336, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Otolaryngology, Eye Ear Nose & Throat Hospital of Fudan University Shanghai 200031, China.
authors.forename: Tian-Yu
authors.initials: TY
authors.lastname: Zhang
authors.affiliation: Department of Otolaryngology, Eye Ear Nose & Throat Hospital of Fudan University Shanghai 200031, China.
authors.forename: Le-Tian
authors.initials: LT
authors.lastname: Tan
authors.affiliation: Department of Otolaryngology, Eye Ear Nose & Throat Hospital of Fudan University Shanghai 200031, China.
authors.forename: Shu-Yi
authors.initials: SY
authors.lastname: Wang
authors.affiliation: Institude of Biochemistry and Cell Biology of Medical College of Shanghai Jiaotong University Shanghai 200025, China.
authors.forename: Yu-Ying
authors.initials: YY
authors.lastname: Chen
authors.affiliation: Department of Otolaryngology, Eye Ear Nose & Throat Hospital of Fudan University Shanghai 200031, China.
authors.forename: Jie-Yan
authors.initials: JY
authors.lastname: Tian
authors.affiliation: Department of Otolaryngology, Eye Ear Nose & Throat Hospital of Fudan University Shanghai 200031, China.
authors.forename: Wen-Ying
authors.initials: WY
authors.lastname: Da
authors.affiliation: Department of Otolaryngology, Tongren Hospital Affiliated to Shanghai Jiaotong University School of Medicine Shanghai 200336, China.
authors.forename: Ping
authors.initials: P
authors.lastname: He
authors.affiliation: Department of Otolaryngology, Tongren Hospital Affiliated to Shanghai Jiaotong University School of Medicine Shanghai 200336, China.
authors.forename: Ya-Ming
authors.initials: YM
authors.lastname: Zhao
keywords: DNA probes; Laryngeal neoplasm; human papilloma; human papillomavirus; papillomavirus