title: A simple, fast and inexpensive method to assess salt stress tolerance of aerial plant part: Investigations in the mandarin group.
abstract: For grafted plants, salt stress tolerance of the aerial plant part is poorly documented. Thus, we developed a simple, fast and inexpensive method to identify tolerant genotypes. Twigs of 14 mandarin accessions that we previously analyzed as seedlings were cut in solution to prevent embolism and were then evaluated in salt stress condition for a week. Physiological parameters such as gas exchanges, leaf Cl(-) and Na(+), as well as the presence of H2O2 and the activity of enzymes involved in ROS synthesis and detoxification processes were analyzed. One accession known to be tolerant as rootstock was shown to be sensitive with limited Cl(-) translocation from the solution to the shoot while sensitive accessions when grown as seedlings presented limited wilting symptoms and accumulated large leaf Cl(-) content. A model is proposed to explain the different strategies of the plant to cope with high toxic ion content. This method allows separation of the root compartment, where ion exclusion mechanisms may exist and have an impact on the salt stress tolerance of the whole plant.
authors.affiliation: CIRAD, UMR AGAP, Avenue Agropolis, TA A-75/02, 34398 Montpellier cedex 5, France; IVIA, Ctra. Moncada-Náquera Km 5, 46113 Moncada, Valencia, Spain.
authors.forename: Jihène
authors.initials: J
authors.lastname: Ben Yahmed
authors.affiliation: CIRAD, UMR AGAP, Avenue Agropolis, TA A-75/02, 34398 Montpellier cedex 5, France.
authors.forename: Tahise M
authors.initials: TM
authors.lastname: de Oliveira
authors.affiliation: IVIA, Ctra. Moncada-Náquera Km 5, 46113 Moncada, Valencia, Spain.
authors.forename: Pedro
authors.initials: P
authors.lastname: Novillo
authors.affiliation: IVIA, Ctra. Moncada-Náquera Km 5, 46113 Moncada, Valencia, Spain.
authors.forename: Anna
authors.initials: A
authors.lastname: Quinones
authors.affiliation: IVIA, Ctra. Moncada-Náquera Km 5, 46113 Moncada, Valencia, Spain.
authors.forename: Maria-Angeles
authors.initials: MA
authors.lastname: Forner
authors.affiliation: IVIA, Ctra. Moncada-Náquera Km 5, 46113 Moncada, Valencia, Spain.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Salvador
authors.affiliation: CIRAD, UMR AGAP, Avenue Agropolis, TA A-75/02, 34398 Montpellier cedex 5, France.
authors.forename: Yann
authors.initials: Y
authors.lastname: Froelicher
authors.affiliation: INAT, 43, Avenue Charles Nicolle 1082, Tunis, Mahrajène, Tunisia.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Ben Mimoun
authors.affiliation: IVIA, Ctra. Moncada-Náquera Km 5, 46113 Moncada, Valencia, Spain.
authors.forename: Manuel
authors.initials: M
authors.lastname: Talon
authors.affiliation: CIRAD, UMR AGAP, Avenue Agropolis, TA A-75/02, 34398 Montpellier cedex 5, France.
authors.forename: Patrick
authors.initials: P
authors.lastname: Ollitrault
authors.affiliation: CIRAD, UMR AGAP, Avenue Agropolis, TA A-75/02, 34398 Montpellier cedex 5, France; IVIA, Ctra. Moncada-Náquera Km 5, 46113 Moncada, Valencia, Spain. Electronic address: raphael.morillon@cirad.fr.
authors.forename: Raphaël
authors.initials: R
authors.lastname: Morillon
mesh_terms: D000383:Agriculture / Q000191:economics / Q000379:methods*; D002957:Citrus / Q000235:genetics / Q000502:physiology*; D005838:Genotype; D018515:Plant Leaves / Q000502:physiology*; D055049:Salt Tolerance; D012965:Sodium Chloride / Q000494:pharmacology*
chemical_list: D012965:Sodium Chloride
keywords: Aerial plant part; Citrus; Mandarin; Salt stress; Varieties