title: Enzymatically triggered rupture of polymersomes.
abstract: Polymersomes are robust vesicles made from amphiphilic block co-polymers. Large populations of uniform giant polymersomes with defined, entrapped species can be made by templating of double-emulsions using microfluidics. In the present study, a series of two enzymatic reactions, one inside and the other outside of the polymersome, were designed to induce rupture of polymersomes. We measured how the kinetics of rupture were affected by altering enzyme concentration. These results suggest that protocells with entrapped enzymes can be engineered to secrete contents on cue.
authors.affiliation: Department of Chemical and Biomolecular Engineering, The University of Pennsylvania, Philadelphia PA, USA. hammer@seas.upenn.edu daeyeon@seas.upenn.edu.
authors.forename: Woo-Sik
authors.initials: WS
authors.lastname: Jang
authors.affiliation: Department of Chemical and Biomolecular Engineering, The University of Pennsylvania, Philadelphia PA, USA. hammer@seas.upenn.edu daeyeon@seas.upenn.edu.
authors.forename: Seung Chul
authors.initials: SC
authors.lastname: Park
authors.affiliation: Department of Chemical and Biomolecular Engineering, The University of Pennsylvania, Philadelphia PA, USA. hammer@seas.upenn.edu daeyeon@seas.upenn.edu.
authors.forename: Ellen H
authors.initials: EH
authors.lastname: Reed
authors.affiliation: Department of Chemical Engineering, Rowan University, Glassboro NJ, USA.
authors.forename: Kevin P
authors.initials: KP
authors.lastname: Dooley
authors.affiliation: Harriton High School, Rosemont PA, USA.
authors.forename: Samuel F
authors.initials: SF
authors.lastname: Wheeler
authors.affiliation: Department of Chemical and Biomolecular Engineering, The University of Pennsylvania, Philadelphia PA, USA. hammer@seas.upenn.edu daeyeon@seas.upenn.edu.
authors.forename: Daeyeon
authors.initials: D
authors.lastname: Lee
authors.affiliation: Department of Chemical and Biomolecular Engineering, The University of Pennsylvania, Philadelphia PA, USA. hammer@seas.upenn.edu daeyeon@seas.upenn.edu and Department of Bioengineering, The University of Pennsylvania, Philadelphia PA, USA.
authors.forename: Daniel A
authors.initials: DA
authors.lastname: Hammer
mesh_terms: D004655:Emulsions / Q000737:chemistry*; D005949:Glucose Oxidase / Q000737:chemistry / Q000378:metabolism*; D008081:Liposomes / Q000737:chemistry; D044085:Microfluidics; D011095:Polyethylenes / Q000737:chemistry*
chemical_list: D004655:Emulsions; D008081:Liposomes; D011095:Polyethylenes; D005949:Glucose Oxidase