title: RNA-seq de novo Assembly Reveals Differential Gene Expression in Glossina palpalis gambiensis Infected with Trypanosoma brucei gambiense vs. Non-Infected and Self-Cured Flies.
abstract: Trypanosoma brucei gambiense (Tbg), causing the sleeping sickness chronic form, completes its developmental cycle within the tsetse fly vector Glossina palpalis gambiensis (Gpg) before its transmission to humans. Within the framework of an anti-vector disease control strategy, a global gene expression profiling of trypanosome infected (susceptible), non-infected, and self-cured (refractory) tsetse flies was performed, on their midguts, to determine differential genes expression resulting from in vivo trypanosomes, tsetse flies (and their microbiome) interactions. An RNAseq de novo assembly was achieved. The assembled transcripts were mapped to reference sequences for functional annotation. Twenty-four percent of the 16,936 contigs could not be annotated, possibly representing untranslated mRNA regions, or Gpg- or Tbg-specific ORFs. The remaining contigs were classified into 65 functional groups. Only a few transposable elements were present in the Gpg midgut transcriptome, which may represent active transpositions and play regulatory roles. One thousand three hundred and seventy three genes differentially expressed (DEGs) between stimulated and non-stimulated flies were identified at day-3 post-feeding; 52 and 1025 between infected and self-cured flies at 10 and 20 days post-feeding, respectively. The possible roles of several DEGs regarding fly susceptibility and refractoriness are discussed. The results provide new means to decipher fly infection mechanisms, crucial to develop anti-vector control strategies.
authors.affiliation: UMR 177, Institut de Recherche Pour le Développement-CIRAD, CIRAD TA A-17/G Montpellier, France.
authors.forename: Illiassou
authors.initials: I
authors.lastname: Hamidou Soumana
authors.affiliation: Institut National de la Recherche Agronomique, GenoToul, UR875 Castanet-Tolosan, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Klopp
authors.affiliation: UMR 177, Institut de Recherche Pour le Développement-CIRAD, CIRAD TA A-17/G Montpellier, France.
authors.forename: Sophie
authors.initials: S
authors.lastname: Ravel
authors.affiliation: Institut National de la Recherche Agronomique, GenoToul, UR875 Castanet-Tolosan, France.
authors.forename: Ibouniyamine
authors.initials: I
authors.lastname: Nabihoudine
authors.affiliation: UMR 177, Institut de Recherche Pour le Développement-CIRAD, CIRAD TA A-17/G Montpellier, France.
authors.forename: Bernadette
authors.initials: B
authors.lastname: Tchicaya
authors.affiliation: Centre National de la Recherche Scientifique, Unité Mixte de Recherche 5203, Institut de Génomique Fonctionnelle Montpellier, France ; Institut National de la Santé et de la Recherche Médicale U661 Montpellier, France ; Universités de Montpellier 1 and 2, UMR 5203 Montpellier, France ; Montpellier GenomiX, Institut de Génomique Fonctionnelle Montpellier, France.
authors.forename: Hugues
authors.initials: H
authors.lastname: Parrinello
authors.affiliation: UMR MIVEGEC (Institut de Recherche pour le Développement 224-Centre National de la Recherche Scientifique 5290-UM1-UM2), Institut de Recherche pour le Développement Montpellier, France.
authors.forename: Luc
authors.initials: L
authors.lastname: Abate
authors.affiliation: Centre National de la Recherche Scientifique, Unité Mixte de Recherche 5203, Institut de Génomique Fonctionnelle Montpellier, France ; Institut National de la Santé et de la Recherche Médicale U661 Montpellier, France ; Universités de Montpellier 1 and 2, UMR 5203 Montpellier, France ; Montpellier GenomiX, Institut de Génomique Fonctionnelle Montpellier, France.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Rialle
authors.affiliation: UMR 177, Institut de Recherche Pour le Développement-CIRAD, CIRAD TA A-17/G Montpellier, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Geiger
keywords: Glossina palpalis gambiensis; RNA-seq; Trypanosoma brucei gambiense; de novo assembly; human African trypanosomiasis; in vivo metatranscriptomics