title: Association of NER pathway gene polymorphisms with susceptibility to laryngeal cancer in a Chinese population.
abstract: We systematically analyzed the association of nine SNPs of seven key NER pathway genes with the development of laryngeal cancer patients, and investigated whether NER pathway polymorphisms could serve as potential biomarkers for laryngeal cancer risk. 271 patients with pathologically proven laryngeal cancer and 271 control subjects were included in our study. Genotyping of ERCC1 rs11615 and rs2298881, ERCC2 rs13181 and rs50871, ERCC3 rs4150441, ERCC4 rs6498486, ERCC5 rs2094258, XPA rs2808668 and XPC rs2228001 were analyzed by polymerase chain reaction (PCR) coupled with restriction fragment length polymorphism (RFLP). By conditional logistic regression analysis, individuals carrying the TT genotype of ERCC1 rs11615 were correlated with an increased risk of larynx cancer when compared with the CC genotype (OR=1.89, 95% CI=1.07-3.37; P value=0.02). Moreover, individuals with the GG genotype of ERCC2 rs50871 were associated with an elevated risk of larynx cancer when compare with the TT genotype (OR=2.03, 95% CI=1.15-3.63; P value=0.01). We found a significant interaction between ERCC2 rs50871 polymorphism and tobacco smoking in the risk of larynx cancer (P for interaction <0.05). In conclusion, our study showed that ERCC1 rs11615 and ERCC2 rs50871 polymorphisms could influence the risk of larynx cancer in Chinese population, particularly among smokers.
authors.affiliation: Department of Otorhinolaryngology, The First Affiliated Hospital of Harbin Medical University Harbin 150001, China.
authors.forename: Yanan
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Department of Medical Oncology, The First Affiliated Hospital of Harbin Medical University Harbin 150001, China.
authors.forename: Lijun
authors.initials: L
authors.lastname: Tan
authors.affiliation: Department of Otorhinolaryngology, The First Affiliated Hospital of Harbin Medical University Harbin 150001, China.
authors.forename: Huijun
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Otorhinolaryngology, The First Affiliated Hospital of Harbin Medical University Harbin 150001, China.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Qin
authors.affiliation: Department of Otorhinolaryngology, The First Affiliated Hospital of Harbin Medical University Harbin 150001, China.
authors.forename: Jiangtao
authors.initials: J
authors.lastname: Liu
mesh_terms: D000368:Aged; D044466:Asian People / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D004720:Endonucleases / Q000235:genetics*; D005260:Female; D005838:Genotype; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D012907:Smoking / Q000009:adverse effects*; D051759:Xeroderma Pigmentosum Group D Protein / Q000235:genetics*
chemical_list: D004268:DNA-Binding Proteins; C071447:ERCC1 protein, human; D004720:Endonucleases; D051759:Xeroderma Pigmentosum Group D Protein; C497292:ERCC2 protein, human
keywords: NER pathway gene; laryngeal cancer; single nucleotide polymorphism