title: Feasibility study with 99mTc-HYNIC-βAla-Bombesin(7-14) as an agent to early visualization of lung tumour cells in nude mice.
abstract: OBJECTIVE
  More sensitive and accurate imaging approaches for early detection and therapy monitoring of lung tumours are needed to ameliorate prognosis and outcome. Lung tumours are known to overexpress receptors for bombesin-like peptides. However, thus far, no study has demonstrated the potential role of bombesin-like peptides in identifying A549 lung tumour cells in xenograft animal models. Thus, we evaluate the feasibility of Tc-HYNIC-βAla-Bombesin(7-14) as an imaging probe in lung cancer.
  
  
  RESULTS
  Xenograft lung tumours were implanted in nude mice and evaluated by histopathological analysis. Tumours were easily visualized by Tc-HYNIC-βAla-Bombesin(7-14) within 30 days after inoculation of the A549 cell line into mice. Scintigraphic images showed high tumour-to-background ratio.
  
  
  CONCLUSIONS
  The data obtained in this study indicate that Tc-HYNIC-βAla-Bombesin(7-14) may be useful as an imaging probe to detect A549 lung cancer cells. To our knowledge, this is the first time that this specific radiocompound has been used to visualize non-small-cell lung cancer A549 in mice. Further translational research in humans is required to establish the potential role of this radiocompound in clinical practice.
authors.affiliation: aDepartment of Pharmacy bDepartment of General Pathology, Biological Sciences Institute, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais cDepartment of Pharmaceutical Sciences, Federal University of Santa Catarina, Florianópolis, Santa Catarina, Brazil dDepartment of Nuclear Medicine, Molecular Imaging, Radiology, NeuroRadiology, Medical Physics, Clinical Laboratory, Microbiology & Pathology, Santa Maria dela Misericordia Hospital, Rovigo, Italy.
authors.forename: Lucas L
authors.initials: LL
authors.lastname: Marostica
authors.forename: André L B
authors.initials: AL
authors.lastname: de Barros
authors.forename: Juliana O
authors.initials: JO
authors.lastname: Silva
authors.forename: Sávia C A
authors.initials: SC
authors.lastname: Lopes
authors.forename: Breno S
authors.initials: BS
authors.lastname: Salgado
authors.forename: Sotirios
authors.initials: S
authors.lastname: Chondrogiannis
authors.forename: Domenico
authors.initials: D
authors.lastname: Rubello
authors.forename: Geovanni D
authors.initials: GD
authors.lastname: Cassali
authors.forename: Eloir P
authors.initials: EP
authors.lastname: Schenkel
authors.forename: Valbert N
authors.initials: VN
authors.lastname: Cardoso
authors.forename: Cláudia M O
authors.initials: CM
authors.lastname: Simões
authors.forename: Mônica C
authors.initials: MC
authors.lastname: Oliveira
mesh_terms: D000072283:A549 Cells; D000409:Alanine / Q000737:chemistry*; D000818:Animals; D001839:Bombesin / Q000031:analogs & derivatives* / Q000737:chemistry* / Q000493:pharmacokinetics; D002471:Cell Transformation, Neoplastic; D005240:Feasibility Studies; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000000981:diagnostic imaging* / Q000473:pathology; D051379:Mice; D008819:Mice, Nude; D015609:Organotechnetium Compounds / Q000737:chemistry*; D014018:Tissue Distribution
chemical_list: D015609:Organotechnetium Compounds; C000606095:technetium 99m HYNIC-beta-Ala-bombesin(7-14); D000409:Alanine; D001839:Bombesin