title: Assessment of thermal treatment via irrigation of external ear to reduce cisplatin-induced hearing loss.
abstract: Systemic and local changes in body temperature can have a profound effect on traumatic injuries including those to the inner ear. Therefore, we investigated the effects of acutely increasing or decreasing the temperature of the external ear canal on cisplatin-induced hearing loss. The external auditory canals of male guinea pigs were acutely irrigated with warm (44 °C), euthermic (37 °C), or cool (30 °C) water and subsequently injected with cisplatin (12 mg/kg, i.p.). Hearing was assessed by the auditory brainstem response and cochleograms were prepared to determine loss of hair cells. Ear canal irrigation with warm water potentiated cisplatin-induced hearing loss and outer hair cell loss whereas cool ear canal irrigation showed significant protection from cisplatin-induced hearing loss and outer hair cell loss. These results suggest that non-invasive cool water ear canal irrigation may be highly effective clinical procedure for protecting against cisplatin-induced hearing loss.
authors.affiliation: Department of Otolaryngology and Communicative Sciences, University of Mississippi Medical Center, USA. Electronic address: cspankovich@umc.edu.
authors.forename: Christopher
authors.initials: C
authors.lastname: Spankovich
authors.affiliation: School of Behavioral and Brain Sciences, University of Texas at Dallas, USA.
authors.forename: Edward
authors.initials: E
authors.lastname: Lobarinas
authors.affiliation: Center for Hearing and Deafness, University at Buffalo, Buffalo, NY 14214, USA.
authors.forename: Dalian
authors.initials: D
authors.lastname: Ding
authors.affiliation: Center for Hearing and Deafness, University at Buffalo, Buffalo, NY 14214, USA.
authors.forename: Richard
authors.initials: R
authors.lastname: Salvi
authors.affiliation: School of Behavioral and Brain Sciences, University of Texas at Dallas, USA.
authors.forename: Colleen G
authors.initials: CG
authors.lastname: Le Prell
mesh_terms: D000818:Animals; D001309:Auditory Threshold; D001833:Body Temperature Regulation; D002945:Cisplatin; D003051:Cochlea / Q000503:physiopathology; D004195:Disease Models, Animal; D004424:Ear Canal / Q000503:physiopathology*; D016057:Evoked Potentials, Auditory, Brain Stem; D006168:Guinea Pigs; D018072:Hair Cells, Auditory, Outer / Q000473:pathology; D006309:Hearing; D034381:Hearing Loss / Q000139:chemically induced / Q000473:pathology / Q000503:physiopathology / Q000517:prevention & control*; D006979:Hyperthermia, Induced / Q000009:adverse effects; D007036:Hypothermia, Induced; D008297:Male; D007507:Therapeutic Irrigation / Q000379:methods*
chemical_list: D002945:Cisplatin
keywords: Cisplatin; Hyperthermia; Hypothermia; Otoprotection; Ototoxicity; Thermal