title: Coassembly of Photosystem II and ATPase as Artificial Chloroplast for Light-Driven ATP Synthesis.
abstract: Adenosine triphosphate (ATP) is one of the most important energy sources in living cells, which can drive serial key biochemical processes. However, generation of a proton gradient for ATP production in an artificial way poses a great challenge. In nature, photophosphorylation occurring in chloroplasts is an ideal prototype of ATP production. In this paper we imitate the light-to-ATP conversion process occurring in the thylakoid membrane by construction of FoF1-ATPase proteoliposome-coated PSII-based microspheres with well-defined core@shell structures using molecular assembly. Under light illumination, PSII can split water into protons, oxygen, and electrons and can generate a proton gradient for ATPase to produce ATP. Thus, an artificially designed chloroplast for PSII-driven ATP synthesis is realized. This biomimetic system will help to understand the photophosphorylation process and may facilitate the development of ATP-driven devices by remote light control.
authors.affiliation: Beijing National Laboratory for Molecular Sciences (BNLMS), CAS Key Lab of Colloid, Interface and Chemical Thermodynamics, Institute of Chemistry, Chinese Academy of Sciences , Beijing 100190, China.
authors.forename: Xiyun
authors.initials: X
authors.lastname: Feng
authors.affiliation: Beijing National Laboratory for Molecular Sciences (BNLMS), CAS Key Lab of Colloid, Interface and Chemical Thermodynamics, Institute of Chemistry, Chinese Academy of Sciences , Beijing 100190, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Jia
authors.affiliation: Beijing National Laboratory for Molecular Sciences (BNLMS), CAS Key Lab of Colloid, Interface and Chemical Thermodynamics, Institute of Chemistry, Chinese Academy of Sciences , Beijing 100190, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Cai
authors.affiliation: Beijing National Laboratory for Molecular Sciences (BNLMS), CAS Key Lab of Colloid, Interface and Chemical Thermodynamics, Institute of Chemistry, Chinese Academy of Sciences , Beijing 100190, China.
authors.forename: Jinbo
authors.initials: J
authors.lastname: Fei
authors.affiliation: Beijing National Laboratory for Molecular Sciences (BNLMS), CAS Key Lab of Colloid, Interface and Chemical Thermodynamics, Institute of Chemistry, Chinese Academy of Sciences , Beijing 100190, China.
authors.forename: Junbai
authors.initials: J
authors.lastname: Li
mesh_terms: D000255:Adenosine Triphosphate / Q000096:biosynthesis*; D040761:Biomimetic Materials / Q000737:chemistry* / Q000378:metabolism; D002736:Chloroplasts / Q000737:chemistry / Q000528:radiation effects / Q000648:ultrastructure; D008027:Light; D008863:Microspheres; D010785:Photophosphorylation / Q000528:radiation effects; D045332:Photosystem II Protein Complex / Q000737:chemistry* / Q000378:metabolism; D011510:Proteolipids / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D006180:Proton-Translocating ATPases / Q000737:chemistry* / Q000378:metabolism; D011522:Protons; D013816:Thermodynamics
chemical_list: D045332:Photosystem II Protein Complex; D011510:Proteolipids; D011522:Protons; C020485:proteoliposomes; D000255:Adenosine Triphosphate; D006180:Proton-Translocating ATPases
keywords: ATP synthase; biomimetic synthesis; coassembly; photosynthesis; photosystem II