title: Development of an Amino Acid-Functionalized Fluorescent Nanocarrier to Deliver a Toxin to Kill Insect Pests.
abstract: Large-scale cultivation of Bacillus thuringiensis Berliner (Bt) crops has led to the rapid development of drug resistance. Herein, a fluorescent star poly(amino acid) is synthesized with l-isoleucine functionalization for the efficient delivery of either positively or negatively charged exogenous proteins into live cells. Poly(amino acid)s (P1)/Cry1Ab complexes greatly increase the cytotoxicity of the Bt toxin, Cry1Ab, and efficiently kill Bt-resistant pests.
authors.affiliation: Department of Entomology, China Agricultural University, 100193, Beijing, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, Key Laboratory of Carbon Fiber and Functional Polymers, Ministry of Education, Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, 100029, Beijing, China.
authors.forename: Shusen
authors.initials: S
authors.lastname: You
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, Key Laboratory of Carbon Fiber and Functional Polymers, Ministry of Education, Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, 100029, Beijing, China.
authors.forename: Chendong
authors.initials: C
authors.lastname: Ji
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, Key Laboratory of Carbon Fiber and Functional Polymers, Ministry of Education, Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, 100029, Beijing, China.
authors.forename: Meizhen
authors.initials: M
authors.lastname: Yin
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, Key Laboratory of Carbon Fiber and Functional Polymers, Ministry of Education, Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, 100029, Beijing, China.
authors.forename: Wantai
authors.initials: W
authors.lastname: Yang
authors.affiliation: Department of Entomology, China Agricultural University, 100193, Beijing, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Shen
mesh_terms: D000596:Amino Acids / Q000737:chemistry*; D000818:Animals; D001427:Bacterial Toxins / Q000737:chemistry* / Q000378:metabolism; D004331:Drosophila melanogaster / Q000378:metabolism; D004337:Drug Carriers / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D007094:Imides / Q000737:chemistry; D008958:Models, Molecular; D049329:Nanostructures / Q000737:chemistry*; D010569:Perylene / Q000031:analogs & derivatives / Q000737:chemistry; D010572:Pest Control, Biological; D011487:Protein Conformation
chemical_list: D000596:Amino Acids; D001427:Bacterial Toxins; D004337:Drug Carriers; D005456:Fluorescent Dyes; D007094:Imides; C521332:perylenediimide; D010569:Perylene
keywords: Bt toxin; Bt-resistant pests; drug resistance; perylenediimide chromophores; protein nanocarriers