title: Bacterial adhesion on biomedical surfaces covered by yttria stabilized zirconia.
abstract: The aim of this study was to compare the bacterial adhesion of Staphylococcus spp. on Ti-6Al-4V with respect to Ti-6Al-V modified alloys with a set of Cubic yttria stabilized zirconia (YSZ) and Ag-YSZ nanocomposite films. Silver is well known to have a natural biocidal character and its presence in the surface predicted to enhance the antimicrobial properties of biomedical surfaces. Microbial adhesion tests were performed using collection strains and twelve clinical strains of Staphylococcus aureus and Staphylococcus epidermidis. The adherence study was performed using a previously published protocol by Kinnari et al. Both collection strains and clinical isolates have shown lower bacterial adhesion to materials modified with respect to the alloy Ti-6Al-4V and the modification with silver reduced the bacterial adhesion for most of all the strains studied. Moreover the percentage of dead bacteria have been evaluated, demonstrating increased proportion of dead bacteria for the modified surfaces. Nanocrystalline silver dissolves releasing both Ag(+) and Ag(0) whereas other silver sources release only Ag(+). We can conclude that YSZ with nanocrystalline silver coating may lead to diminished postoperative infections and to increased corrosion and scratch resistance of YSZ incorporating alloys Ti-6Al-4V.
authors.affiliation: Department of Clinical Microbiology, IIS-Fundación Jiménez Díaz, Ave Reyes Católicos 2, 28040, Madrid, Spain. ramontanoira@hotmail.com.|Otorhinolaryngology-Head and Neck Surgery, University of Helsinki and Helsinki University Hospital, Helsinki, Finland. ramontanoira@hotmail.com.
authors.forename: Ramón
authors.initials: R
authors.lastname: Pérez-Tanoira
authors.affiliation: Institut Jean Lamour, UMR7198, Université de Lorraine, 54011, Nancy, France.
authors.forename: David
authors.initials: D
authors.lastname: Horwat
authors.affiliation: Otorhinolaryngology-Head and Neck Surgery, University of Helsinki and Helsinki University Hospital, Helsinki, Finland.
authors.forename: Teemu J
authors.initials: TJ
authors.lastname: Kinnari
authors.affiliation: Department of Clinical Microbiology, IIS-Fundación Jiménez Díaz, Ave Reyes Católicos 2, 28040, Madrid, Spain.
authors.forename: Concepción
authors.initials: C
authors.lastname: Pérez-Jorge
authors.affiliation: Department of Traumatology and Orthopaedic, IIS-La Paz, 28046, Madrid, Spain.
authors.forename: Enrique
authors.initials: E
authors.lastname: Gómez-Barrena
authors.affiliation: Centre de Compétences en Microscopies Electroniques et Microsondes (CC-MEM), Institut Jean Lamour, Parc de Saurupt, CS 50840, 54011, Nancy, France.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Migot
authors.affiliation: Department of Clinical Microbiology, IIS-Fundación Jiménez Díaz, Ave Reyes Católicos 2, 28040, Madrid, Spain.
authors.forename: Jaime
authors.initials: J
authors.lastname: Esteban
mesh_terms: D001422:Bacterial Adhesion; D008855:Microscopy, Electron, Scanning; D013499:Surface Properties; D015019:Yttrium / Q000737:chemistry*; D015040:Zirconium / Q000737:chemistry*
chemical_list: D015019:Yttrium; D015040:Zirconium; C028541:zirconium oxide