title: [The Role of Cancer-associated Fibroblasts in Invasive Behavior of Pituitary Adenoma].
abstract: OBJECTIVE
  To determine the role of cancer associated fibroblasts (CAFs) in the invasive behavior of pituitary adenoma.
  
  
  METHODS
  Pituitary adenoma tissues were divided into invasive group (IPA) and non-invasive group (nIPA) according to pre-operative MRI and observations during surgery. Those tissues were cultured and CAFs were identified through a smooth muscle actin (α-SMA). The migratory and invasive ability of CAFs was tested with transwell migration and invasion assay. The expressions of α-SMA and matrix metalloproteinase (MMP)-9 from CAFs were determined by immunocytochemistry and Western blot.
  
  
  RESULTS
  All cultured CAFs expressed α-SMA. No significant difference in migratory ability of CAFs was found between the IPA and nIPA tissues; however, CAFs from the IPA tissues had stronger invasive ability than those from the nIPA tissues (P= 0. 010). Higher levels of MMP-9 expression were found in group IPA as compared with nIPA (P=0. 025). No significant difference in the expression of α-SMA was found between the two groups.
  
  
  CONCLUSIONS
  CAFs may promote invasive behavior by secreting more MMP-9, which may play a part in the invasive behavior of pituitary adenomas.
authors.forename: Chuan-hong
authors.initials: CH
authors.lastname: Zhong
authors.forename: Bei
authors.initials: B
authors.lastname: Tao
authors.forename: Yao
authors.initials: Y
authors.lastname: Wu
authors.forename: Li-lei
authors.initials: LL
authors.lastname: Peng
authors.forename: Pei-zhi
authors.initials: PZ
authors.lastname: Zhou
authors.forename: Li-gang
authors.initials: LG
authors.lastname: Chen
authors.forename: Shu
authors.initials: S
authors.lastname: Jiang
mesh_terms: D000199:Actins / Q000378:metabolism; D000236:Adenoma / Q000473:pathology*; D015153:Blotting, Western; D002465:Cell Movement; D005347:Fibroblasts / Q000166:cytology*; D006801:Humans; D007150:Immunohistochemistry; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D009361:Neoplasm Invasiveness; D010911:Pituitary Neoplasms / Q000473:pathology*
chemical_list: C541116:ACTA2 protein, human; D000199:Actins; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9