title: Lack of association between the insulin receptor substrates-1 Gly972Arg polymorphism and type-2 diabetes mellitus among Saudis from Eastern Saudi Arabia.
abstract: OBJECTIVE
  To investigate the association between  the insulin receptor substrate-1 (IRS1) Gly972Arg polymorphism and type-2 diabetes mellitus (T2DM) among Saudis from Eastern Saudi Arabia.
  
  
  METHODS
  This study was conducted between May and December 2014 at King Fahad Hospital of the University, Al-Khobar, Kingdom of Saudi Arabia. In a case-control study design, a total of 143 subjects (age range: 35-73 years) comprising 74 healthy controls and 69 patients with T2DM were examined. Blood samples were collected from subjects and subjected to genomic DNA extraction and chemical analysis. The IRS1 Gly972Arg polymorphism was then genotyped using the standard polymerase chain reaction-restriction fragment length polymorphism technique.
  
  
  RESULTS
  Eight out of 74 (10.8%) of the control group carried at least one copy of the mutated allele. The frequency (8.7%) of the IRS1 variant was also found in the diabetic group. Logistic regression analysis showed an adjusted odds ratio of 1.04, 95% confidence interval 0.28 - 3.95, and a p-value of 0.94.
  
  
  CONCLUSIONS
  We failed to find any association between the IRS1 Gly972Arg polymorphism and T2DM.
authors.affiliation: Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, University of Dammam, Dammam, Kingdom of Saudi Arabia. E-mail. hawra.alsalman@gmail.com.
authors.forename: Hawra A
authors.initials: HA
authors.lastname: Alsalman
authors.forename: Yahia A
authors.initials: YA
authors.lastname: Kaabi
mesh_terms: D000328:Adult; D000368:Aged; D001120:Arginine / Q000235:genetics*; D016022:Case-Control Studies; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics*; D005260:Female; D005998:Glycine / Q000235:genetics*; D006801:Humans; D055504:Insulin Receptor Substrate Proteins / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D012529:Saudi Arabia
chemical_list: D055504:Insulin Receptor Substrate Proteins; D001120:Arginine; D005998:Glycine