title: Changes of ferrous iron and its transporters after intracerebral hemorrhage in rats.
abstract: OBJECTIVE
  Ferrous iron is a major source inducing oxidative stress after intracerebral hemorrhage (ICH). Divalent metal transporter1 (DMT1) is the important and well-known plasma membrane transport protein which was proved to be involved in the transport of free ferrous iron in mammals. Ferroportin 1 (FPN1) is the unique exporter of ferrous iron from mammalian cells. The role of DMT1 and FPN1 in brain after ICH is still not elucidated. Therefore, we measure the expression of DMT1 and FPN1, to explore the correlations between ferrous iron and its specific transporters after ICH.
  
  
  METHODS
  Ninety-six Sprague-Dawley rats received intra-striatal infusions of 0.5 U type IV collagenase to establish ICH model. Ferrous iron content in brain was determined using Turnbull's method. DMT1 and FPN1 expression were examined by immunohistochemical staining and Real-Time quantitative polymerase chain reaction (RT-PCR). With the use of confocal laser microscopy, we determined the colocalization of DMT1 and FPN1 at 1, 3, 7 and 14 days after ICH.
  
  
  RESULTS
  Ferrous iron deposition was shown in the perihematomal zone as early as 1 day after ICH; it reached a peak after 7 days and was not elevated within 14 days following ICH. The expression of the DMT1 upregulated and reached to peak at day 7 after ICH. FPN1 reached a plateau at 3 days post-ICH. Expression levels of DMT1 and FPN1 were in parallel with ferrous iron deposition. There was a positive correlation between FPN1 and DMT1. DMT1 mainly localized in the cytoplasm of glias and neurons. FPN1 were mostly distributed on the membrane of endothelial cells and glias. Confocal microscope showed that DMT1 colocalized with FPN1.
  
  
  CONCLUSIONS
  DMT1 and FPN1 are positively influenced by ferrous iron status in brain after ICH. DMT1 and FPN1 attenuate iron overload after ICH via increasing transmembrane iron export.
authors.affiliation: Department of Neurology, The Second Hospital, Shanxi Medical University 382 Wuyi Avenue, Taiyuan 030001, Shanxi, China.
authors.forename: Gaiqing
authors.initials: G
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, Second Affiliated Hospital, School of Medicine, Zhejiang University 88 Jiefang Road, Hangzhou 310009, Zhejiang, China.
authors.forename: Anwen
authors.initials: A
authors.lastname: Shao
authors.affiliation: Department of Neurology, The Second Hospital, Shanxi Medical University 382 Wuyi Avenue, Taiyuan 030001, Shanxi, China.
authors.forename: Weimin
authors.initials: W
authors.lastname: Hu
authors.affiliation: Department of Neurology, The Second Hospital, Shanxi Medical University 382 Wuyi Avenue, Taiyuan 030001, Shanxi, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Xue
authors.affiliation: Department of Neurology, Shanxi Large Hospital Taiyuan 030000, Shanxi,China.
authors.forename: Hongping
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Department of Neurology, Tianjin First Center Hospital Tianjin 300192, China.
authors.forename: Xiaojie
authors.initials: X
authors.lastname: Jin
authors.affiliation: Department of Neurology, The Second Hospital, Shanxi Medical University 382 Wuyi Avenue, Taiyuan 030001, Shanxi, China.
authors.forename: Guanglai
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Neurology, The Second Hospital, Shanxi Medical University 382 Wuyi Avenue, Taiyuan 030001, Shanxi, China.
authors.forename: Zhitang
authors.initials: Z
authors.lastname: Sun
authors.affiliation: Department of Neurology, The Second Hospital, Shanxi Medical University 382 Wuyi Avenue, Taiyuan 030001, Shanxi, China.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
mesh_terms: D000818:Animals; D001692:Biological Transport; D001921:Brain / Q000378:metabolism* / Q000473:pathology; D027682:Cation Transport Proteins / Q000235:genetics / Q000378:metabolism*; D002543:Cerebral Hemorrhage / Q000139:chemically induced / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D017364:Collagenases; D004195:Disease Models, Animal; D007501:Iron / Q000378:metabolism*; D008297:Male; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D000098742:Ferroportin; D000099236:Solute Carrier Family 11, Member 2
chemical_list: D027682:Cation Transport Proteins; D017364:Collagenases; D007501:Iron; D000098742:Ferroportin; D000099236:Solute Carrier Family 11, Member 2
keywords: Ferrous iron; divalent metal transporter1; ferroportin1; intracerebral hemorrhage