title: Filling the Green Gap of a Megadalton Photosystem I Complex by Conjugation of Organic Dyes.
abstract: Photosynthesis is Nature's major process for converting solar into chemical energy. One of the key players in this process is the multiprotein complex photosystem I (PSI) that through absorption of incident photons enables electron transfer, which makes this protein attractive for applications in bioinspired photoactive hybrid materials. However, the efficiency of PSI is still limited by its poor absorption in the green part of the solar spectrum. Inspired by the existence of natural phycobilisome light-harvesting antennae, we have widened the absorption spectrum of PSI by covalent attachment of synthetic dyes to the protein backbone. Steady-state and time-resolved photoluminescence reveal that energy transfer occurs from these dyes to PSI. It is shown by oxygen-consumption measurements that subsequent charge generation is substantially enhanced under broad and narrow band excitation. Ultimately, surface photovoltage (SPV) experiments prove the enhanced activity of dye-modified PSI even in the solid state.
authors.forename: Pavlo I
authors.initials: PI
authors.lastname: Gordiichuk
authors.affiliation: The Bio and Molecular Electronics Group, Department of Materials Science and Engineering, Faculty of Engineering and University Center for Nano Science and Nanotechnology, Tel Aviv University , Tel-Aviv, 69978, Israel.
authors.forename: Dolev
authors.initials: D
authors.lastname: Rimmerman
authors.forename: Avishek
authors.initials: A
authors.lastname: Paul
authors.forename: Daniel A
authors.initials: DA
authors.lastname: Gautier
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Gruszka
authors.forename: Manfred
authors.initials: M
authors.lastname: Saller
authors.forename: Jan Willem
authors.initials: JW
authors.lastname: de Vries
authors.forename: Gert-Jan A H
authors.initials: GJ
authors.lastname: Wetzelaer
authors.forename: Marianna
authors.initials: M
authors.lastname: Manca
authors.forename: Widianta
authors.initials: W
authors.lastname: Gomulya
authors.affiliation: Department of Materials Engineering and the Ilze Katz Institute for Nanoscale Science and Technology, Ben Gurion University of the Negev , Beer-Sheva, Israel.
authors.forename: Maayan
authors.initials: M
authors.lastname: Matmor
authors.affiliation: The Bio and Molecular Electronics Group, Department of Materials Science and Engineering, Faculty of Engineering and University Center for Nano Science and Nanotechnology, Tel Aviv University , Tel-Aviv, 69978, Israel.
authors.forename: Ekaterina
authors.initials: E
authors.lastname: Gloukhikh
authors.forename: Mark
authors.initials: M
authors.lastname: Loznik
authors.affiliation: Department of Materials Engineering and the Ilze Katz Institute for Nanoscale Science and Technology, Ben Gurion University of the Negev , Beer-Sheva, Israel.
authors.forename: Nurit
authors.initials: N
authors.lastname: Ashkenasy
authors.affiliation: Molecular Electronics Group, Max Planck Institute for Polymer Research , Ackermannweg 10, 55128 Mainz, Germany.
authors.forename: Paul W M
authors.initials: PW
authors.lastname: Blom
authors.affiliation: Plant Biochemistry, Ruhr University Bochum , D-44780 Bochum, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Rögner
authors.forename: Maria Antonietta
authors.initials: MA
authors.lastname: Loi
authors.affiliation: The Bio and Molecular Electronics Group, Department of Materials Science and Engineering, Faculty of Engineering and University Center for Nano Science and Nanotechnology, Tel Aviv University , Tel-Aviv, 69978, Israel.
authors.forename: Shachar
authors.initials: S
authors.lastname: Richter
authors.forename: Andreas
authors.initials: A
authors.lastname: Herrmann
mesh_terms: D000458:Cyanobacteria / Q000737:chemistry; D004735:Energy Transfer; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry*; D006576:Heterocyclic Compounds, 4 or More Rings / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D049449:Luminescence; D008239:Lysine / Q000737:chemistry; D046529:Microscopy, Electron, Transmission; D010100:Oxygen / Q000737:chemistry / Q000378:metabolism; D045331:Photosystem I Protein Complex / Q000737:chemistry*
chemical_list: C521550:Atto590; D005456:Fluorescent Dyes; D006576:Heterocyclic Compounds, 4 or More Rings; D045331:Photosystem I Protein Complex; D008239:Lysine; D010100:Oxygen