title: Evaluation of the long-term imprecision of the Capillarys 2 Flex Piercing® with serial differences in patient Hemoblogin A1c data: A comparison with two common immunoassays.
abstract: Since HbA1c became a diagnostic criterion for type 2 diabetes, assays measuring this analyte were required to be analytically stronger. Although precision of Sebia Capillarys 2 Flex Piercing® was previously evaluated with standard methods and material, we wanted to document the long-term performance of this analyzer in two different centers.
  
  
  METHODS
  In this study, we evaluated, with a non-conventional methodology, the analytical precision of the Sebia Capillarys 2 Flex Piercing® using HbA1c patient data and compared it with two different common commercial immunoassays.
  
  
  RESULTS
  CV of 1.33% and 1.32% was obtained in the two centers for the electrophoretic technique compared to 2.66% and 3.92% for the Roche Cobas Integra 800® and Beckman Synchron DxC® immunoassays technique respectively.
  
  
  CONCLUSIONS
  Our results show that capillary electrophoresis is more robust than the 2 immunoassays tested for long-term precision and even exceeds minimal requirements recommended.
authors.affiliation: Département de biologie médicale, Centre Intégré Universitaire de Santé et de Services Sociaux du Saguenay-Lac-St-Jean, Hôpital de Chicoutimi, Saguenay, Québec, Canada. Electronic address: Renee.Guerin.csssc@ssss.gouv.qc.ca.
authors.forename: Renée
authors.initials: R
authors.lastname: Guérin
authors.affiliation: Département de biologie médicale, Centre Intégré de Santé et de Services Sociaux des Laurentides, Hôpital régional de St-Jérôme, St-Jérôme, Québec, Canada.
authors.forename: Samuel
authors.initials: S
authors.lastname: Dugré-Brisson
mesh_terms: D019075:Electrophoresis, Capillary / Q000379:methods*; D006442:Glycated Hemoglobin / Q000032:analysis*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D015203:Reproducibility of Results
chemical_list: D006442:Glycated Hemoglobin; C517652:hemoglobin A1c protein, human
keywords: Capillary electrophoresis; Capillarys 2 flex piercing®; Hemoglobin A1c; Long-term imprecision