title: Purification of Early and Late Endosomes.
abstract: Proteomic analysis of early and late endosomes has been constrained by the limited purity of the endosomal fractions that can be achieved by biochemical methods. Here we briefly review endocytic pathways, and then introduce fractionation strategies that have been used to improve the purity of isolated endosomes. In addition, we describe innovative proteomics analysis methods that have been shown to partially circumvent the limitations found in the enrichment steps.
authors.affiliation: Biocenter, Division of Cell Biology, Innsbruck Medical University, A-6020 Innsbruck, Austria.
authors.forename: Mariana E G
authors.initials: ME
authors.lastname: de Araújo
authors.affiliation: Biocenter, Division of Cell Biology, Innsbruck Medical University, A-6020 Innsbruck, Austria.
authors.forename: Giorgia
authors.initials: G
authors.lastname: Lamberti
authors.affiliation: Biocenter, Division of Cell Biology, Innsbruck Medical University, A-6020 Innsbruck, Austria.
authors.forename: Lukas A
authors.initials: LA
authors.lastname: Huber
mesh_terms: D000818:Animals; D002458:Cell Fractionation / Q000379:methods*; D011992:Endosomes / Q000737:chemistry*; D006801:Humans; D020543:Proteome / Q000032:analysis* / Q000302:isolation & purification*
chemical_list: D020543:Proteome