title: HTR1B gene variants associate with the susceptibility of Raynauds' phenomenon in workers exposed hand-arm vibration.
abstract: OBJECTIVE
  To explore whether polymorphic variants of the HTR1B gene are associated with the susceptibility of Raynauds' Phenomenon (RP) coursed by vibration.
  
  
  METHODS
  148 subjects exposed to vibration for more than 2 years were classified into either induced white finger (VWF) group (n = 72), or non-VWF group (n = 76). Vibration exposure levels were measured and assessed following ISO 5349-1:2001 protocol. All workers were genotyped by sequencing for the single nucleotide polymorphisms (SNPs) in the 5'-flanking and coding region of HTR1B. Genetic characteristics and linkage disequilibrium (LD) were analyzed with Haploview. Serum serotonin levels of each subject were detected using ELISA. The association between the susceptibility of vascular damage and genotype was analyzed via logistic regression.
  
  
  RESULTS
  7 known SNPs were obtained and their allele frequencies were inserted into the Hardy-Weinberg equilibrium. rs6297 variant genotype had an increased risk of VWF compared with wild genotype (OR = 2.14, 95% CI = 1.04- 4.58, P < 0.05). rs6298 mutant type (AG+GG) was found to have a significant interaction on vibration exposure LN(CEI), accounting for VWF occurrence. LN(5-HT) level is significantly different between the VWF group (x¯±s= 1.99±1.09 ng/mL) and the non-VWF group (x¯±s= 2.72±1.47 ng/mL).
  
  
  CONCLUSIONS
  Serotonin levels may affect the progression of secondary RP. Polymorphic variants of the HTR1B gene are associated with the susceptibility of secondary RP in vibration-exposed occupational populations of Chinese Han people.
authors.affiliation: Guangdong Prevention and Cure Centre of Occupational Diseases, Guangzhou, China.
authors.forename: Qingsong
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Guangdong Prevention and Cure Centre of Occupational Diseases, Guangzhou, China.
authors.forename: Li
authors.initials: L
authors.lastname: Lang
authors.affiliation: Guangdong Prevention and Cure Centre of Occupational Diseases, Guangzhou, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Xiao
authors.affiliation: Guangdong Prevention and Cure Centre of Occupational Diseases, Guangzhou, China.
authors.forename: Hansheng
authors.initials: H
authors.lastname: Lin
authors.affiliation: Guangdong Prevention and Cure Centre of Occupational Diseases, Guangzhou, China.
authors.forename: Aichu
authors.initials: A
authors.lastname: Yang
authors.affiliation: Guangdong Prevention and Cure Centre of Occupational Diseases, Guangzhou, China.
authors.forename: Hongling
authors.initials: H
authors.lastname: Li
authors.affiliation: Key Laboratory of Occupational Health and Safety, Beijing Municipal Institute of Labor Protection, Beijing, China.
authors.forename: Shichuan
authors.initials: S
authors.lastname: Tang
authors.affiliation: Guangdong Prevention and Cure Centre of Occupational Diseases, Guangzhou, China.
authors.forename: Hanlin
authors.initials: H
authors.lastname: Huang
mesh_terms: D000328:Adult; D001132:Arm / Q000473:pathology*; D005260:Female; D020022:Genetic Predisposition to Disease; D006225:Hand / Q000473:pathology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009784:Occupational Diseases / Q000235:genetics* / Q000473:pathology; D020641:Polymorphism, Single Nucleotide; D011928:Raynaud Disease / Q000235:genetics* / Q000473:pathology; D044302:Receptor, Serotonin, 5-HT1B / Q000235:genetics*; D014732:Vibration / Q000009:adverse effects*
chemical_list: C470302:HTR1B protein, human; D044302:Receptor, Serotonin, 5-HT1B
keywords: HTR1B; Raynauds’ Phenomenon (RP); single nucleotide polymorphisms; vascular damage; vibration-induced white finger (VWF)