title: Potential Wound Healing Activities of Galla Rhois in Human Fibroblasts and Keratinocytes.
abstract: Wound healing is a complex process orchestrated by the regeneration of the epithelium and the remodeling of the extracellular matrix through processes like collagen deposition. Galla Rhois has been widely used in traditional Korean medicine for its various pharmacological effects, including an anticoccidial effect, however, little is known about its healing activity. The purpose of this study was to determine the effects of Galla Rhois ethanol extract (GRE) on wound healing activities, including H2O2-induced oxidative stress, cell migration, and lactate dehydrogenase (LDH) release assays using human keratinocyte (HaCaT) and dermal fibroblasts (CCD-986SK). In addition, total soluble collagen deposition and collagen gene expression for Type I and III collagen were evaluated in CCD-986SK. Total tannin and flavonoid contents for GRE were measured. GRE induced a significant increase in the number and migration of cells, along with a decrease in cell death and LDH release. In addition, it also induced the over-expression of collagen Type I and III mRNA and caused increased synthesis of total soluble collagen. The contents of total tannin and flavonoid for GRE were 55.7% ([Formula: see text][Formula: see text]mg/g) and 62.9% ([Formula: see text][Formula: see text]mg/g), respectively. The results suggest that GRE can cause accelerated wound healing by increasing cell survival, proliferation, migration, and collagen synthesis along with a potential anti-oxidant property. This evidence provides novel insight into natural therapy for tissue injury.
authors.affiliation: 1 Research and Development Division, Korea Promotion Institute for Traditional Medicine Industry, Gyeongsan 712-260, Republic of Korea.
authors.forename: Hyo-Hyun
authors.initials: HH
authors.lastname: Park
authors.affiliation: 1 Research and Development Division, Korea Promotion Institute for Traditional Medicine Industry, Gyeongsan 712-260, Republic of Korea.
authors.forename: Na-Young
authors.initials: NY
authors.lastname: Park
authors.affiliation: 1 Research and Development Division, Korea Promotion Institute for Traditional Medicine Industry, Gyeongsan 712-260, Republic of Korea.
authors.forename: Sun-Gun
authors.initials: SG
authors.lastname: Kim
authors.affiliation: 1 Research and Development Division, Korea Promotion Institute for Traditional Medicine Industry, Gyeongsan 712-260, Republic of Korea.
authors.forename: Kyu-Tae
authors.initials: KT
authors.lastname: Jeong
authors.affiliation: 1 Research and Development Division, Korea Promotion Institute for Traditional Medicine Industry, Gyeongsan 712-260, Republic of Korea.
authors.forename: Eu-Jin
authors.initials: EJ
authors.lastname: Lee
authors.affiliation: 1 Research and Development Division, Korea Promotion Institute for Traditional Medicine Industry, Gyeongsan 712-260, Republic of Korea.
authors.forename: Eunkyung
authors.initials: E
authors.lastname: Lee
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis; D004848:Epithelium / Q000502:physiology; D005109:Extracellular Matrix / Q000378:metabolism / Q000502:physiology; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology*; D016166:Free Radical Scavengers; D006430:Hemiptera; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology; D008517:Phytotherapy; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D012038:Regeneration / Q000187:drug effects; D029045:Rhus / Q000737:chemistry* / Q000469:parasitology; D012867:Skin / Q000166:cytology; D013268:Stimulation, Chemical; D013634:Tannins; D014945:Wound Healing / Q000187:drug effects*; D014947:Wounds and Injuries / Q000188:drug therapy / Q000503:physiopathology
chemical_list: D016166:Free Radical Scavengers; D010936:Plant Extracts; D013634:Tannins; D003094:Collagen; D007770:L-Lactate Dehydrogenase
keywords: Collagen; Fibroblast; Galla Rhois; Keratinocyte; Oxidative Stress; Wound Healing