title: Mesenchymal stem cell-conditioned medium accelerates wound healing with fewer scars.
abstract: Mesenchymal stem cells (MSCs) derived from umbilical cords (UC-MSCs) have been shown to enhance cutaneous wound healing by means of the paracrine activity. Fibroblasts are the primary cells involved in wound repair. The paracrine effects of UC-MSCs on dermal fibroblasts have not been fully explored in vitro or in vivo. Dermal fibroblasts were treated with conditioned media from UC-MSCs (UC-MSC-CM). In this model, UC-MSC-CM increased the proliferation and migration of dermal fibroblasts. Moreover, adult dermal fibroblasts transitioned into a phenotype with a low myofibroblast formation capacity, a decreased ratio of transforming growth factor-β1,3 (TGF-β1/3) and an increased ratio of matrix metalloproteinase/tissue inhibitor of metalloproteinases (MMP/TIMP). Additionally, UC-MSC-CM-treated wounds showed accelerated healing with fewer scars compared with control groups. These observations suggest that UC-MSC-CM may be a feasible strategy to promote cutaneous repair and a potential means to realise scarless healing.
authors.affiliation: Wound Healing and Cell Biology Laboratory, Institute of Basic Medical Science, Chinese PLA General Hospital, Beijing, P. R. China.|Trauma Treatment Center, Central Laboratory, Chinese PLA General Hospital Hainan Branch, Sanya, P. R. China.
authors.forename: Meirong
authors.initials: M
authors.lastname: Li
authors.affiliation: Trauma Treatment Center, Central Laboratory, Chinese PLA General Hospital Hainan Branch, Sanya, P. R. China.
authors.forename: Fuxin
authors.initials: F
authors.lastname: Luan
authors.affiliation: Trauma Treatment Center, Central Laboratory, Chinese PLA General Hospital Hainan Branch, Sanya, P. R. China.
authors.forename: Yali
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Wound Healing and Cell Biology Laboratory, Institute of Basic Medical Science, Chinese PLA General Hospital, Beijing, P. R. China.
authors.forename: Haojie
authors.initials: H
authors.lastname: Hao
authors.affiliation: Wound Healing and Cell Biology Laboratory, Institute of Basic Medical Science, Chinese PLA General Hospital, Beijing, P. R. China.
authors.forename: Jiejie
authors.initials: J
authors.lastname: Liu
authors.affiliation: Wound Healing and Cell Biology Laboratory, Institute of Basic Medical Science, Chinese PLA General Hospital, Beijing, P. R. China.
authors.forename: Liang
authors.initials: L
authors.lastname: Dong
authors.affiliation: Wound Healing and Cell Biology Laboratory, Institute of Basic Medical Science, Chinese PLA General Hospital, Beijing, P. R. China.
authors.forename: Xiaobing
authors.initials: X
authors.lastname: Fu
authors.affiliation: Wound Healing and Cell Biology Laboratory, Institute of Basic Medical Science, Chinese PLA General Hospital, Beijing, P. R. China.
authors.forename: Weidong
authors.initials: W
authors.lastname: Han
mesh_terms: D049109:Cell Proliferation / Q000502:physiology*; D017077:Culture Media, Conditioned; D005347:Fibroblasts / Q000502:physiology*; D006801:Humans; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D014945:Wound Healing / Q000502:physiology*
chemical_list: D017077:Culture Media, Conditioned
keywords: Fibroblast; Mesenchymal stem cell-conditioned medium; Scarless; TGF-β