title: Enzymatic Hydrolysis of Hydrotropic Pulps at Different Substrate Loadings.
abstract: Enzymatic hydrolysis of cellulosic raw materials to produce nutrient broths for microbiological synthesis of ethanol and other valuable products is an important field of modern biotechnology. Biotechnological processing implies the selection of an effective pretreatment technique for raw materials. In this study, the hydrotropic treatment increased the reactivity of the obtained substrates toward enzymatic hydrolysis by 7.1 times for Miscanthus and by 7.3 times for oat hulls. The hydrotropic pulp from oat hulls was more reactive toward enzymatic hydrolysis compared to that from Miscanthus, despite that the substrates had similar compositions. As the initial substrate loadings were raised during enzymatic hydrolysis of the hydrotropic Miscanthus and oat hull pulps, the concentration of reducing sugars increased by 34 g/dm(3) and the yield of reducing sugars decreased by 31 %. The findings allow us to predict the efficiency of enzymatic hydrolysis of hydrotropic pulps from Miscanthus and oat hulls when scaling up the process by volume.
authors.affiliation: Laboratory of Bioconversion, Institute for Problems of Chemical and Energetic Technologies, Siberian Branch of the Russian Academy of Sciences (IPCET SB RAS), 1 Socialisticheskaya St, Biysk, 659322, Altai Krai, Russia. bioconversion@mail.ru.
authors.forename: Marina N
authors.initials: MN
authors.lastname: Denisova
authors.affiliation: Laboratory of Bioconversion, Institute for Problems of Chemical and Energetic Technologies, Siberian Branch of the Russian Academy of Sciences (IPCET SB RAS), 1 Socialisticheskaya St, Biysk, 659322, Altai Krai, Russia.
authors.forename: Ekaterina I
authors.initials: EI
authors.lastname: Makarova
authors.affiliation: Laboratory of Bioconversion, Institute for Problems of Chemical and Energetic Technologies, Siberian Branch of the Russian Academy of Sciences (IPCET SB RAS), 1 Socialisticheskaya St, Biysk, 659322, Altai Krai, Russia.
authors.forename: Igor N
authors.initials: IN
authors.lastname: Pavlov
authors.affiliation: Laboratory of Bioconversion, Institute for Problems of Chemical and Energetic Technologies, Siberian Branch of the Russian Academy of Sciences (IPCET SB RAS), 1 Socialisticheskaya St, Biysk, 659322, Altai Krai, Russia.
authors.forename: Vera V
authors.initials: VV
authors.lastname: Budaeva
authors.affiliation: Laboratory of Bioconversion, Institute for Problems of Chemical and Energetic Technologies, Siberian Branch of the Russian Academy of Sciences (IPCET SB RAS), 1 Socialisticheskaya St, Biysk, 659322, Altai Krai, Russia.
authors.forename: Gennady V
authors.initials: GV
authors.lastname: Sakovich
mesh_terms: D002480:Cellulase / Q000737:chemistry*; D002482:Cellulose / Q000737:chemistry; D006868:Hydrolysis; D008855:Microscopy, Electron, Scanning; D013379:Substrate Specificity
chemical_list: D002482:Cellulose; D002480:Cellulase
keywords: Enzymatic hydrolysis; Hydrotropic treatment; Miscanthus; Oat hulls; Pulp