title: Inhibitory effect of microwaved thinned nectarine extracts on polyphenol oxidase activity.
abstract: By-products from agricultural practices or from the fruit processing industry are a source of bioactive compounds that could be used in the food industry. Such by-products include thinned fruits, which are expected to contain high quantities of interesting compounds. One possible application of this fruits is the prevention of the enzymatic browning suffered by fruits and vegetables after minimal processing. The aim of this study is to determine the in vitro and in vivo activity of microwaved extracts obtained from thinned nectarines. It has been observed that in vitro the extracts obtained after the application of high microwave power levels (500, 1000 and 1500 W) are mixed type inhibitors of polyphenoloxidase enzyme, showing an irreversible inactivation. This inhibition could be attributed to the Maillard reaction products formed during the microwave treatment. In vivo, a solution of 2% of the extract obtained at 1500 W inhibited the enzymatic browning in minimally processed peaches for 8 days of storage.
authors.affiliation: Plant Food Research Group, Instituto Agroalimentario de Aragón-IA2 - (Universidad de Zaragoza-CITA), Miguel Servet, 177, 50013, Spain.
authors.forename: Diego
authors.initials: D
authors.lastname: Redondo
authors.affiliation: Plant Food Research Group, Instituto Agroalimentario de Aragón-IA2 - (Universidad de Zaragoza-CITA), Miguel Servet, 177, 50013, Spain. Electronic address: ugeventu@unizar.es.
authors.forename: María E
authors.initials: ME
authors.lastname: Venturini
authors.affiliation: Plant Food Research Group, Instituto Agroalimentario de Aragón-IA2 - (Universidad de Zaragoza-CITA), Miguel Servet, 177, 50013, Spain.
authors.forename: Rosa
authors.initials: R
authors.lastname: Oria
authors.affiliation: Aula Dei Scientific and Technological Park, Avda. Montañana 930, 50059 Zaragoza, Spain.
authors.forename: Esther
authors.initials: E
authors.lastname: Arias
mesh_terms: D004156:Catechol Oxidase / Q000737:chemistry* / Q000378:metabolism; D005514:Food Irradiation; D005638:Fruit / Q000737:chemistry*; D015416:Maillard Reaction; D008872:Microwaves; D010084:Oxidation-Reduction; D010940:Plant Proteins / Q000737:chemistry* / Q000378:metabolism; D000068239:Prunus persica / Q000737:chemistry / Q000201:enzymology* / Q000528:radiation effects*
chemical_list: D010940:Plant Proteins; D004156:Catechol Oxidase
keywords: Browning; Fresh-cut peach; Maillard; Polyphenol oxidase; Thinned