title: Transcriptional profiles discriminate patients with pulmonary tuberculosis from non-tuberculous individuals depending on the presence of non-insulin diabetes mellitus.
abstract: Our objective was to identify transcriptional biomarkers in peripheral blood mononuclear cells (PBMC) that discriminate individuals with latent tuberculosis infection (LTBI) from those with pulmonary tuberculosis (PTB) in subjects with non-insulin-dependent diabetes mellitus (NIDDM) and in individuals without NIDDM. Using gene expression microarrays we identified differentially expressed genes from lungs of mice infected with Mycobacterium tuberculosis (Mtb) or a mutant (ΔsigH) representing a non-inflammatory model. Genes expressed in blood, with inflammatory related functions were evaluated in humans by RT-qPCR. NCF1 and ORM transcripts have the better discriminatory capacity to identify PTB subjects from LTBI and non-infected controls (NICs) independently of the presence of NIDDM. The sequential evaluation of the mRNA levels of NCF1 and ORM as multiple diagnostic tests showed 95% Sensitivity (Se) and 80% Specificity (Sp). In addition, FPR2 promises to be a good biomarker for the PTB detection in subjects with NIDDM (Se=100%; Sp=90%).
authors.affiliation: BioMedical Research Unit of Zacatecas, Mexican Institute of Social Security (IMSS), Zacatecas, Mexico.
authors.forename: Carmen J
authors.initials: CJ
authors.lastname: Serrano
authors.affiliation: BioMedical Research Unit of Zacatecas, Mexican Institute of Social Security (IMSS), Zacatecas, Mexico.
authors.forename: Betzaida
authors.initials: B
authors.lastname: Cuevas-Córdoba
authors.affiliation: BioMedical Research Unit of Zacatecas, Mexican Institute of Social Security (IMSS), Zacatecas, Mexico; Department of Immunology, Faculty of Medicine, Autonomous University of San Luis Potosí (UASLP), SLP, Mexico.
authors.forename: Noé
authors.initials: N
authors.lastname: Macías-Segura
authors.affiliation: BioMedical Research Unit of Zacatecas, Mexican Institute of Social Security (IMSS), Zacatecas, Mexico.
authors.forename: Rosa Angélica
authors.initials: RA
authors.lastname: González-Curiel
authors.affiliation: BioMedical Research Unit of Zacatecas, Mexican Institute of Social Security (IMSS), Zacatecas, Mexico.
authors.forename: Víctor Yordani
authors.initials: VY
authors.lastname: Martínez-Balderas
authors.affiliation: BioMedical Research Unit of Zacatecas, Mexican Institute of Social Security (IMSS), Zacatecas, Mexico.
authors.forename: Leonor
authors.initials: L
authors.lastname: Enciso-Moreno
authors.affiliation: TB Program, Bill and Melinda Gates Foundation, Seattle, USA.
authors.forename: Peter
authors.initials: P
authors.lastname: Small
authors.affiliation: Experimental Pathology Section, Department of Pathology, National Institute of Medical Sciences and Nutrition Salvador Zubirán, Mexico City, Mexico.
authors.forename: Rogelio
authors.initials: R
authors.lastname: Hernández-Pando
authors.affiliation: BioMedical Research Unit of Zacatecas, Mexican Institute of Social Security (IMSS), Zacatecas, Mexico. Electronic address: enciso_2000@yahoo.com.
authors.forename: José Antonio
authors.initials: JA
authors.lastname: Enciso-Moreno
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D015415:Biomarkers; D016000:Cluster Analysis; D003924:Diabetes Mellitus, Type 2 / Q000150:complications*; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D008297:Male; D051379:Mice; D008875:Middle Aged; D014397:Tuberculosis, Pulmonary / Q000150:complications* / Q000175:diagnosis* / Q000503:physiopathology
chemical_list: D015415:Biomarkers
keywords: Biomarkers; Diabetes; Transcriptional profile; Tuberculosis