title: Fabrication of PEG-carboxymethylcellulose hydrogel by thiol-norbornene photo-click chemistry.
abstract: Both poly(ethylene glycol) (PEG) and carboxymethylcellulose (CMC) are biocompatible polymers, which have been widely utilized in biomedical fields. In this study, we demonstrated the fabrication of pH-sensitive PEG-CMC hydrogels based on thiol-norbornene photo-click reaction and characterized their properties, such as swelling ratio, stiffness, degradation, and protein drug release. For the hydrogel fabrication, tetra-arm PEG and CMC were functionalized with norbornene groups and thiol groups, respectively. The hydrogels fabricated with varying concentration (0-3%) of CMCSH and a fixed concentration (4%) of PEG4NB by orthogonal step-growth photopolymerization showed high gel fractions (∼0.85). The presence of CMCSH in hydrogel did not affected gel point (∼4 s) but significantly prolonged completion time of gelation. Swelling ratios of PEG-CMC increased from ∼32 to ∼60 and the shear elastic modulus decreased from ∼3000 to ∼600 Pa with an increase of CMCSH content (0-3%). PEG-CMC hydrogel containing more CMCSH not only underwent slower hydrolytic bulk degradation but also showed a slower BSA release in acidic condition. These results indicate thiol-norbornene PEG-CMC hydrogel has potential as pH-sensitive protein drug carrier.
authors.affiliation: Department of Biosystems and Biomaterials Science and Engineering, Seoul National University, Seoul 08826, Republic of Korea.
authors.forename: Sora
authors.initials: S
authors.lastname: Lee
authors.affiliation: Department of Biosystems and Biomaterials Science and Engineering, Seoul National University, Seoul 08826, Republic of Korea.
authors.forename: Young Hwan
authors.initials: YH
authors.lastname: Park
authors.affiliation: Department of Biosystems and Biomaterials Science and Engineering, Seoul National University, Seoul 08826, Republic of Korea. Electronic address: ki.cs@snu.ac.kr.
authors.forename: Chang Seok
authors.initials: CS
authors.lastname: Ki
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry; D002266:Carboxymethylcellulose Sodium / Q000737:chemistry*; D057930:Click Chemistry / Q000379:methods; D003432:Cross-Linking Reagents / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry; D055119:Elastic Modulus; D020100:Hydrogels / Q000737:chemistry*; D006868:Hydrolysis; D011092:Polyethylene Glycols / Q000737:chemistry*; D013438:Sulfhydryl Compounds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D004337:Drug Carriers; D020100:Hydrogels; D013438:Sulfhydryl Compounds; D011092:Polyethylene Glycols; D002266:Carboxymethylcellulose Sodium
keywords: Carboxymethylcellulose; Hydrogel; Photopolymerization