title: Biomolecular Interactions of Tannin Isolated from Oenothera gigas with Liposomes.
abstract: We have examined the interaction between hydrolysable tannin 1-O-galloyl-4,6-hexahydroxydiphenoyl-β-D-glucose (OGβDG) with neutral liposomes as a model of cell membranes composed of three lipids: lecithin, 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) at different mass ratios. OGβDG in the concentration range 0.5-15 µg/ml (0.4-12 µM) strongly interacts with liposomal membranes by changing their structure, surface charge and fluidity. Used OGβDG molecules decrease and increase the rigidity of hydrophilic surface and hydrophobic parts of liposomes, respectively. At higher concentrations of tannin (>15 µM), liposomes are aggregated. Fourier Transform Infra-Red (FTIR) analysis showed that mainly -OH groups from OGβDG and also PO(2-) groups from phospholipids are responsible for the interaction. Obtained data indicate the importance of membrane lipid composition in interactions between tannins and cells.
authors.affiliation: Department of Biophysics, Laboratory of Molecular Biophysics, Faculty of Biology and Chemistry, University of Bialystok, 15-950, Bialystok, Poland. sekowski100@vp.pl.
authors.forename: Szymon
authors.initials: S
authors.lastname: Sekowski
authors.affiliation: Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, 90-236, Lodz, Poland.
authors.forename: Maksim
authors.initials: M
authors.lastname: Ionov
authors.affiliation: Department of Natural Products Chemistry, Faculty of Biology and Chemistry, University of Bialystok, 15-950, Bialystok, Poland.|Bio-Nano-Techno Center, Faculty of Biology and Chemistry, University of Bialystok, 15-950, Bialystok, Poland.
authors.forename: Alina
authors.initials: A
authors.lastname: Dubis
authors.affiliation: Institute of Bioorganic Chemistry, Academy of Science of Republic of Uzbekistan, Tashkent-143, Uzbekistan.
authors.forename: Saidmukhtar
authors.initials: S
authors.lastname: Mavlyanov
authors.affiliation: Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, 90-236, Lodz, Poland.
authors.forename: Maria
authors.initials: M
authors.lastname: Bryszewska
authors.affiliation: Department of Biophysics, Laboratory of Molecular Biophysics, Faculty of Biology and Chemistry, University of Bialystok, 15-950, Bialystok, Poland.
authors.forename: Maria
authors.initials: M
authors.lastname: Zamaraeva
mesh_terms: D047348:Hydrolyzable Tannins / Q000737:chemistry; D008051:Lipid Bilayers / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry*; D008560:Membrane Fluidity; D015394:Molecular Structure; D031664:Oenothera / Q000737:chemistry*; D010316:Particle Size; D010743:Phospholipids / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification; D017550:Spectroscopy, Fourier Transform Infrared; D013634:Tannins / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D047348:Hydrolyzable Tannins; D008051:Lipid Bilayers; D008081:Liposomes; D010743:Phospholipids; D010936:Plant Extracts; D013634:Tannins
keywords: Fluorescence; Liposomes; Tannins; Zeta potential