title: Angiogenic Type I Collagen Extracellular Matrix Integrated with Recombinant Bacteriophages Displaying Vascular Endothelial Growth Factors.
abstract: Here, a growth-factor-integrated natural extracellular matrix of type I collagen is presented that induces angiogenesis. The developed matrix adapts type I collagen nanofibers integrated with synthetic colloidal particles of recombinant bacteriophages that display vascular endothelial growth factor (VEGF). The integration is achieved during or after gelation of the type I collagen and the matrix enables spatial delivery of VEGF into a desired region. Endothelial cells that contact the VEGF are found to invade into the matrix to form tube-like structures both in vitro and in vivo, proving the angiogenic potential of the matrix.
authors.affiliation: School of Mechanical Engineering, Korea University Anam Dong, Seongbuk, Seoul, 136-701, Korea.
authors.forename: Junghyo
authors.initials: J
authors.lastname: Yoon
authors.affiliation: Nanomedicine, Korea Institute of Science and Technology in Europe, Uni-Campus Nord E 71, 66123, Saarbrücken, Germany.
authors.forename: Nuriye
authors.initials: N
authors.lastname: Korkmaz Zirpel
authors.affiliation: Department of Biotechnology, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul, 120-749, Korea.
authors.forename: Hyun-Ji
authors.initials: HJ
authors.lastname: Park
authors.affiliation: The California Institute for Quantitative Biosciences, University of California Berkeley, Berkeley, CA, 94720, USA.
authors.forename: Sewoon
authors.initials: S
authors.lastname: Han
authors.affiliation: PLC Inc. Rm 701, Star valley, Gasan-dong, Geumcheon-gu, Seoul, 153-777, Korea.
authors.forename: Kyung Hoon
authors.initials: KH
authors.lastname: Hwang
authors.affiliation: Department of Biotechnology, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul, 120-749, Korea.
authors.forename: Jisoo
authors.initials: J
authors.lastname: Shin
authors.affiliation: Department of Biotechnology, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul, 120-749, Korea.
authors.forename: Seung-Woo
authors.initials: SW
authors.lastname: Cho
authors.affiliation: School of Undergraduate Science, Daegu Gyeongbuk Institute of Science and Technology, 333, Techno jungang-daero, Hyeonpung-myeon, Dalseong-gun, Daegu, 711-873, Korea.
authors.forename: Chang-Hoon
authors.initials: CH
authors.lastname: Nam
authors.affiliation: School of Mechanical Engineering, Korea University Anam Dong, Seongbuk, Seoul, 136-701, Korea.
authors.forename: Seok
authors.initials: S
authors.lastname: Chung
mesh_terms: D000818:Animals; D001435:Bacteriophages / Q000378:metabolism*; D061505:Cell Surface Display Techniques; D024042:Collagen Type I / Q000494:pharmacology*; D004797:Enzyme-Linked Immunosorbent Assay; D005109:Extracellular Matrix / Q000187:drug effects / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D006801:Humans; D008807:Mice, Inbred BALB C; D008856:Microscopy, Fluorescence; D057139:Nanofibers / Q000737:chemistry; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D015202:Protein Engineering; D051381:Rats; D011995:Recombination, Genetic / Q000235:genetics*; D042442:Vascular Endothelial Growth Factors / Q000378:metabolism*
chemical_list: D024042:Collagen Type I; D042442:Vascular Endothelial Growth Factors
keywords: angiogenesis; bacteriophages; collagen; growth factors, VEGF