title: Supramolecular Au(I)-Cu(I) Complexes as New Luminescent Labels for Covalent Bioconjugation.
abstract: Two new supramolecular organometallic complexes, namely, [Au6Cu2(C2C6H4CHO)6(PPh2C6H4PPh2)3](PF6)2 and [Au6Cu2(C2C6H4NCS)6(PPh2C6H4PPh2)3](PF6)2, with highly reactive aldehyde and isothiocyanate groups have been synthesized and characterized using X-ray crystallography, ESI mass spectrometry, and NMR spectroscopy. The compounds obtained demonstrated bright emission in solution with the excited-state lifetime in microsecond domain both under single- and two-photon excitation. The luminescent complexes were found to be suitable for bioconjugation in aqueous media. In particular, they are able to form the covalent conjugates with proteins of different molecular size (soybean trypsin inhibitor, human serum albumin, rabbit anti-HSA antibodies). The conjugates demonstrated a high level of the phosphorescent emission from the covalently bound label, excellent solubility, and high stability in physiological media. The highest quantum yield, storage stability, and luminance were detected for bioconjugates formed by covalent attachment of the aldehyde-bearing supramolecular Au(I)-Cu(I) complex. The measured biological activity of one of the labeled model proteins clearly showed that introduced label did not prevent the biorecognition and specific protein-protein complex formation that was extremely important for the application of the conjugates in biomolecular detection and imaging.
authors.affiliation: St. Petersburg State University , Institute of Chemistry, Universitesky pr. 26, 198504 St. Petersburg, Russia.
authors.forename: Andrei A
authors.initials: AA
authors.lastname: Belyaev
authors.affiliation: St. Petersburg State University , Institute of Chemistry, Universitesky pr. 26, 198504 St. Petersburg, Russia.
authors.forename: Dmitrii V
authors.initials: DV
authors.lastname: Krupenya
authors.affiliation: St. Petersburg State University , Institute of Chemistry, Universitesky pr. 26, 198504 St. Petersburg, Russia.
authors.forename: Elena V
authors.initials: EV
authors.lastname: Grachova
authors.affiliation: St. Petersburg State University , Institute of Earth Sciences, 199034 St. Petersburg, Russia.
authors.forename: Vladislav V
authors.initials: VV
authors.lastname: Gurzhiy
authors.affiliation: St. Petersburg State University , Department of Physics, 198504 St. Petersburg, Russia.|Institute of Nanobiotechnologies, Peter the Great St. Petersburg Polytechnic University , 195251 St. Petersburg, Russia.
authors.forename: Alexei S
authors.initials: AS
authors.lastname: Melnikov
authors.affiliation: St. Petersburg State University , Department of Physics, 198504 St. Petersburg, Russia.|Institute of Nanobiotechnologies, Peter the Great St. Petersburg Polytechnic University , 195251 St. Petersburg, Russia.
authors.forename: Pavel Yu
authors.initials: PY
authors.lastname: Serdobintsev
authors.affiliation: St. Petersburg State University , Institute of Chemistry, Universitesky pr. 26, 198504 St. Petersburg, Russia.
authors.forename: Ekaterina S
authors.initials: ES
authors.lastname: Sinitsyna
authors.affiliation: St. Petersburg State University , Institute of Chemistry, Universitesky pr. 26, 198504 St. Petersburg, Russia.
authors.forename: Evgenia G
authors.initials: EG
authors.lastname: Vlakh
authors.affiliation: St. Petersburg State University , Institute of Chemistry, Universitesky pr. 26, 198504 St. Petersburg, Russia.
authors.forename: Tatiana B
authors.initials: TB
authors.lastname: Tennikova
authors.affiliation: St. Petersburg State University , Institute of Chemistry, Universitesky pr. 26, 198504 St. Petersburg, Russia.
authors.forename: Sergey P
authors.initials: SP
authors.lastname: Tunik
mesh_terms: D000818:Animals; D000906:Antibodies / Q000737:chemistry / Q000378:metabolism; D056831:Coordination Complexes / Q000138:chemical synthesis* / Q000737:chemistry; D003300:Copper / Q000737:chemistry*; D018360:Crystallography, X-Ray; D006046:Gold / Q000737:chemistry*; D006801:Humans; D017879:Isothiocyanates / Q000737:chemistry; D049408:Luminescent Agents / Q000737:chemistry* / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D011817:Rabbits; D012709:Serum Albumin / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization; D014360:Trypsin Inhibitor, Kunitz Soybean / Q000737:chemistry / Q000378:metabolism
chemical_list: D000906:Antibodies; D056831:Coordination Complexes; D017879:Isothiocyanates; D049408:Luminescent Agents; D012709:Serum Albumin; C037152:isothiocyanic acid; D006046:Gold; D003300:Copper; D014360:Trypsin Inhibitor, Kunitz Soybean