title: Development of an orthotopic model of human metastatic prostate cancer in the NOD-SCIDγ mouse (Mus musculus) anterior prostate.
abstract: Prostate cancer is one of the most prevalent oncological diseases in males worldwide, and the mortalities resulting from this type of cancer are mainly due to metastasis. The most common models for the study of metastasis are transgenic and immunocompromised mice, which enable the study of the metastatic process in a controlled way by the injection of prostate cancer cells into the mice. In the present study, NOD-SCIDγ mice were injected orthotopically with PC3 cells in the anterior prostate in order to establish a metastatic model. The results demonstrated the development and growth of a primary tumor that preceded the formation of micrometastases in the lung, liver and pancreas, followed by macrometastases in the liver. This model adequately represents the dynamics of the metastatic process, and may be useful for novel therapeutic assays and post-surgical relapse studies.
authors.affiliation: Laboratory of Molecular and Cellular Andrology, Physiology and Biophysics Program, Institute of Biomedical Sciences, Faculty of Medicine, University of Chile, Santiago 8380453, Chile ; Department of Animal Pathology, Faculty of Veterinary and Animal Sciences, University of Chile, Santiago 8820808, Chile.
authors.forename: Federico F
authors.initials: FF
authors.lastname: Cifuentes
authors.affiliation: Laboratory of Molecular and Cellular Andrology, Physiology and Biophysics Program, Institute of Biomedical Sciences, Faculty of Medicine, University of Chile, Santiago 8380453, Chile.
authors.forename: Rodrigo H
authors.initials: RH
authors.lastname: Valenzuela
authors.affiliation: Laboratory of Molecular and Cellular Andrology, Physiology and Biophysics Program, Institute of Biomedical Sciences, Faculty of Medicine, University of Chile, Santiago 8380453, Chile.
authors.forename: Héctor R
authors.initials: HR
authors.lastname: Contreras
authors.affiliation: Laboratory of Molecular and Cellular Andrology, Physiology and Biophysics Program, Institute of Biomedical Sciences, Faculty of Medicine, University of Chile, Santiago 8380453, Chile.
authors.forename: Enrique A
authors.initials: EA
authors.lastname: Castellón
keywords: NOD-SCIDγ mouse; anterior prostate; metastasis; primary tumor; prostate cancer