title: DNA induced sequestration of a bioactive cationic fluorophore from the lipid environment: A spectroscopic investigation.
abstract: The effect of calf-thymus DNA (ctDNA) on the lipid bound probe, formed by the cationic phenazinium dye phenosafranin (PSF) and the anionic lipid dimyristoyl-L-α-phosphatidylglycerol (DMPG), has been unearthed exploiting various spectroscopic techniques. Steady state and time-resolved fluorometric studies and measurements of circular dichroism and DNA helix melting temperature reveal that in the presence of DNA the probe is dislodged from the lipid environment and gets intercalated within the DNA helix. The work qualitatively illustrates that the anionic lipid can be used as a potential nanocarrier for delivering the cationic drugs to the most relevant biomacromolecular target, DNA.
authors.affiliation: Department of Chemistry, Jadavpur University, Kolkata 700 032, India.
authors.forename: Saptarshi
authors.initials: S
authors.lastname: Ghosh
authors.affiliation: Department of Chemistry, Jadavpur University, Kolkata 700 032, India.
authors.forename: Pronab
authors.initials: P
authors.lastname: Kundu
authors.affiliation: Department of Chemistry, Jadavpur University, Kolkata 700 032, India. Electronic address: nitin.chattopadhyay@yahoo.com.
authors.forename: Nitin
authors.initials: N
authors.lastname: Chattopadhyay
mesh_terms: D000818:Animals; D002412:Cations / Q000737:chemistry; D002417:Cattle; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D010619:Phenazines / Q000737:chemistry; D010715:Phosphatidylglycerols / Q000737:chemistry; D044366:Transition Temperature
chemical_list: D002412:Cations; D005456:Fluorescent Dyes; D010619:Phenazines; D010715:Phosphatidylglycerols; C011977:phenosafranine; D004247:DNA; C427203:calf thymus DNA; C002773:dimyristoylphosphatidylglycerol
keywords: Circular dichroism; Fluorescence; Helix melting; Lipid; Phenosafanin; ctDNA