title: Application of genetically engineered microbial whole-cell biosensors for combined chemosensing.
abstract: The progress of genetically engineered microbial whole-cell biosensors for chemosensing and monitoring has been developed in the last 20 years. Those biosensors respond to target chemicals and produce output signals, which offer a simple and alternative way of assessment approaches. As actual pollution caused by human activities usually contains a combination of different chemical substances, how to employ those biosensors to accurately detect real contaminant samples and evaluate biological effects of the combined chemicals has become a realistic object of environmental researches. In this review, we outlined different types of the recent method of genetically engineered microbial whole-cell biosensors for combined chemical evaluation, epitomized their detection performance, threshold, specificity, and application progress that have been achieved up to now. We also discussed the applicability and limitations of this biosensor technology and analyzed the optimum conditions for their environmental assessment in a combined way.
authors.affiliation: College of Life Sciences, Nanjing Normal University, Nanjing, 210023, China.
authors.forename: Wei
authors.initials: W
authors.lastname: He
authors.affiliation: College of Life Sciences, Nanjing Normal University, Nanjing, 210023, China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Yuan
authors.affiliation: College of Geography Sciences, Nanjing Normal University, Nanjing, 210023, China.
authors.forename: Wen-Hui
authors.initials: WH
authors.lastname: Zhong
authors.affiliation: Department of Genetics and Plant Breeding, Sher-e-Bangla Agricultural University, Dhaka, 1207, Bangladesh.
authors.forename: Md Ashaduzzaman
authors.initials: MA
authors.lastname: Siddikee
authors.affiliation: College of Life Sciences, Nanjing Normal University, Nanjing, 210023, China. daichuanchao@njnu.edu.cn.
authors.forename: Chuan-Chao
authors.initials: CC
authors.lastname: Dai
mesh_terms: D001419:Bacteria / Q000235:genetics* / Q000378:metabolism*; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D004785:Environmental Pollutants / Q000032:analysis* / Q000378:metabolism; D005818:Genetic Engineering
chemical_list: D004785:Environmental Pollutants
keywords: Bioavailability; Combined chemicals; Engineered microbial whole-cell biosensor