title: Hydrogen peroxide-induced secondary necrosis in conidia of Aspergillus fumigatus.
abstract: Aspergillus fumigatus conidia have been linked to severe aspergillosis in immunocompromised patients. Recently, the cytotoxic effect of secondary metabolites from A. fumigatus conidia was reported. In the present work, a methodology used to detect cell death markers in fungal hyphae was adapted to study conidia cell death. Additionally, the mechanism of H2O2-induced cell death was studied in A. fumigatus conidia for the first time. Data presented in this work reveal that the H2O2-induced conidial cell death was associated with a marked increase of TUNEL- and PI-positive cells. It is therefore suggested that conidia cell death occurs in a dose-dependent manner through a secondary necrosis mechanism. The knowledge of conidia cell death machinery may provide insights into the molecular mechanism of conidia-mediated toxicity to the respiratory tract and may pave the way for improved therapeutic approaches against A. fumigatus conidia-mediated diseases.
authors.affiliation: a Instituto de Investigação e Inovação em Saúde, Universidade Do Porto, Portugal.|b Instituto de Patologia e Imunologia Molecular da Universidade do Porto, Porto, Portugal.|c Departamento de Biologia, Faculdade de Ciências, Universidade do Porto, Porto, Portugal.
authors.forename: M
authors.initials: M
authors.lastname: Oliveira
authors.affiliation: d UCIBIO/REQUIMTE, Laboratório de Microbiologia, Departamento de Ciências Biológicas, Faculdade de Farmácia, Universidade do Porto, Rua Jorge Viterbo Ferreira, 228; 4050-313 Porto, Portugal.
authors.forename: C
authors.initials: C
authors.lastname: Pereira
authors.affiliation: d UCIBIO/REQUIMTE, Laboratório de Microbiologia, Departamento de Ciências Biológicas, Faculdade de Farmácia, Universidade do Porto, Rua Jorge Viterbo Ferreira, 228; 4050-313 Porto, Portugal.
authors.forename: C
authors.initials: C
authors.lastname: Bessa
authors.affiliation: b Instituto de Patologia e Imunologia Molecular da Universidade do Porto, Porto, Portugal.
authors.forename: R
authors.initials: R
authors.lastname: Araujo
authors.affiliation: d UCIBIO/REQUIMTE, Laboratório de Microbiologia, Departamento de Ciências Biológicas, Faculdade de Farmácia, Universidade do Porto, Rua Jorge Viterbo Ferreira, 228; 4050-313 Porto, Portugal.
authors.forename: L
authors.initials: L
authors.lastname: Saraiva
mesh_terms: D001232:Aspergillus fumigatus / Q000187:drug effects*; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D020287:In Situ Nick-End Labeling; D009336:Necrosis; D013172:Spores, Fungal / Q000187:drug effects / Q000502:physiology
chemical_list: D006861:Hydrogen Peroxide
keywords: Aspergillus fumigatus; conidia; conidies; hydrogen peroxide; nécrose secondaire; peroxyde d’hydrogène; secondary necrosis