title: Grb7 gene amplification and protein expression by FISH and IHC in ovarian cancer.
abstract: OBJECTIVE
  Overexpression of growth factor receptor-bound protein 7 (Grb7) has been found in numerous human cancers. The aim of this study was to evaluate the correlation between Grb7 gene amplification and protein expression in ovarian cancer (OC).
  
  
  METHODS
  We use Tissue Microarray (TMA) respectively to detect the gene amplification and protein expression of Grb7 in 90 cases OC and 10 control specimens of normal ovarian tissues by IHC and FISH.
  
  
  RESULTS
  The Grb7 protein expression by IHC analysis was observed in 52/90 (57.8%) OC with 3 cases (3.3%) scored 3(+) and 9 cases (10%) scored 2(+) Grb7 gene amplification by FISH analysis was successfully detectable in 6 specimens with a positive rate of 6.8% (6/88) in which immunostaining 3(+), 2(+) and negative (1(+)/0) expressions of Grb7 were 100.0% (3/3), 11.1% (1/9) and 2.6% (2/76), respectively. Our data exhibited that the IHC and FISH results had a good consistency between Grb7 gene amplification and Grb7 protein expression (Kappa = 0.651, P < 0.001). Both the results of IHC and FISH revealed that Grb7 did not seem to have a role in OC clinicopathology.
  
  
  CONCLUSIONS
  There is a close relationship between Grb7 gene amplification and GRB7 protein overexpression in human OC. IHC might have limited diagnostic value especially in these tumors and especially in characterizing genetically diverse borderline cases, FISH could be superior to IHC.
authors.affiliation: Department of Gynecology, The Second Affiliated Hospital of Chongqing Medical University Chongqing, P. R. China.
authors.forename: Manman
authors.initials: M
authors.lastname: Zeng
authors.affiliation: Department of Gynecology, The Second Affiliated Hospital of Chongqing Medical University Chongqing, P. R. China.
authors.forename: Zhu
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Department of Gynecology, The Second Affiliated Hospital of Chongqing Medical University Chongqing, P. R. China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Hu
authors.affiliation: Department of Gynecology, The Second Affiliated Hospital of Chongqing Medical University Chongqing, P. R. China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Gynecology, The Second Affiliated Hospital of Chongqing Medical University Chongqing, P. R. China.
authors.forename: Xiaotao
authors.initials: X
authors.lastname: Yang
authors.affiliation: Department of Gynecology, The Second Affiliated Hospital of Chongqing Medical University Chongqing, P. R. China.
authors.forename: Hailong
authors.initials: H
authors.lastname: Ran
authors.affiliation: Molecular Medical Laboratory, Chongqing Medical University Chongqing, P. R. China.
authors.forename: Yanan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Molecular Medical Laboratory, Chongqing Medical University Chongqing, P. R. China.
authors.forename: Xu
authors.initials: X
authors.lastname: Li
authors.affiliation: Molecular Medical Laboratory, Chongqing Medical University Chongqing, P. R. China.
authors.forename: Qiubo
authors.initials: Q
authors.lastname: Yu
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000032:analysis* / Q000235:genetics*; D016022:Case-Control Studies; D005260:Female; D051382:GRB7 Adaptor Protein / Q000032:analysis* / Q000235:genetics*; D005784:Gene Amplification; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D008875:Middle Aged; D010051:Ovarian Neoplasms / Q000737:chemistry* / Q000235:genetics* / Q000473:pathology; D011237:Predictive Value of Tests; D046888:Tissue Array Analysis; D015854:Up-Regulation; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; C495955:GRB7 protein, human; D051382:GRB7 Adaptor Protein
keywords: Ovarian cancer; fluorescence in situ hybridization; growth factor receptor-bound protein 7 (Grb7); immunohistochemistry; tissue microarray