title: Advenella alkanexedenssp. nov., an alkane-degrading bacterium isolated from biogas slurry samples.
abstract: A novel aerobic bacterium, designated strain LAM0050T, was isolated from a biogas slurry sample, which had been enriched with diesel oil for 30 days. Cells of strain LAM0050Twere gram-stain-negative, non-motile, non-spore-forming and coccoid-shaped. The optimal temperature and pH for growth were 30-35 °C and 8.5, respectively. The strain did not require NaCl for growth, but tolerated up to 5.3 % (w/v) NaCl. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain LAM0050Twas a member of the genusAdvenella, and was most closely related toAdvenella faeciporciKCTC 23732T,Advenella incenataCCUG 45225T,Advenella kashmirensisDSM 17095TandAdvenella mimigardefordensisDSM 17166T, with 98.1, 96.6, 96.6 and 96.3 % sequence similarity, respectively. The DNA-DNA hybridization relatedness between strain LAM0050TandA. faeciporciKCTC 23732Twas 41.7 ± 2.4 %. The genomic DNA G+C content was 51.2 mol%, as determined by theTmmethod. The major fatty acids of strain LAM0050Twere C16 : 0, C17 : 0cyclo, summed feature 3 (C16 : 1ω7cand/or C16 : 1ω6c) and summed feature 8 (C18 : 1ω7cand/or C18 : 1ω6c). The predominant ubiquinone was Q-8. The main polar lipids were diphosphatidyglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine and four unidentified phospholipids. Based on the phenotypic and genotypic properties, strain LAM0050Tis suggested to represent a novel species of the genusAdvenella, for which the nameAdvenella alkanexedenssp. nov., is proposed, the type strain is LAM0050T( = ACCC 06485T= JCM 30465T).
authors.affiliation: Key Laboratory of Microbial Resources (Ministry of Agriculture, PR China), Institute of Agricultural Resources and Regional Planning, CAAS, Beijing 100081, PR China.|Key Laboratory of Development and Application of Rural Renewable Energy (MOA, PR China), Biogas Institute of Ministry of Agriculture, Chengdu 610041, PR China.
authors.forename: Huimin
authors.initials: H
authors.lastname: Wang
authors.affiliation: Key Laboratory of Microbial Resources (Ministry of Agriculture, PR China), Institute of Agricultural Resources and Regional Planning, CAAS, Beijing 100081, PR China.
authors.forename: Shan
authors.initials: S
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Development and Application of Rural Renewable Energy (MOA, PR China), Biogas Institute of Ministry of Agriculture, Chengdu 610041, PR China.
authors.forename: Yanwei
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Laboratory of Microbial Resources (Ministry of Agriculture, PR China), Institute of Agricultural Resources and Regional Planning, CAAS, Beijing 100081, PR China.
authors.forename: Delong
authors.initials: D
authors.lastname: Kong
authors.affiliation: Key Laboratory of Microbial Resources (Ministry of Agriculture, PR China), Institute of Agricultural Resources and Regional Planning, CAAS, Beijing 100081, PR China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Guo
authors.affiliation: Key Laboratory of Microbial Resources (Ministry of Agriculture, PR China), Institute of Agricultural Resources and Regional Planning, CAAS, Beijing 100081, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Key Laboratory of Microbial Resources (Ministry of Agriculture, PR China), Institute of Agricultural Resources and Regional Planning, CAAS, Beijing 100081, PR China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Dong
authors.affiliation: Key Laboratory of Microbial Resources (Ministry of Agriculture, PR China), Institute of Agricultural Resources and Regional Planning, CAAS, Beijing 100081, PR China.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Ruan