title: Parallel Syntheses of Peptides on Teflon-Patterned Paper Arrays (SyntArrays).
abstract: Screening of peptides to find the ligands that bind to specific targets is an important step in drug discovery. These high-throughput screens require large number of structural variants of peptides to be synthesized and tested. This chapter describes the generation of arrays of peptides on Teflon-patterned sheets of paper. First, the protocol describes the patterning of paper with a Teflon solution to produce arrays with solvophobic barriers that are able to confine organic solvents. Next, we describe the parallel syntheses of 96 peptides on Teflon-patterned arrays using the SPOT synthesis method.
authors.affiliation: Department of Chemistry, Alberta Glycomics Center, University of Alberta, 11227 Saskatchewan Drive, Edmonton, AB, Canada, T6G 2G2.
authors.forename: Frédérique
authors.initials: F
authors.lastname: Deiss
authors.affiliation: Department of Chemistry, Alberta Glycomics Center, University of Alberta, 11227 Saskatchewan Drive, Edmonton, AB, Canada, T6G 2G2.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Chemistry, Alberta Glycomics Center, University of Alberta, 11227 Saskatchewan Drive, Edmonton, AB, Canada, T6G 2G2. ratmir.derda@ualberta.ca.
authors.forename: Ratmir
authors.initials: R
authors.lastname: Derda
mesh_terms: D000409:Alanine / Q000737:chemistry; D060326:Chemistry Techniques, Synthetic / Q000379:methods*; D000431:Ethanol / Q000737:chemistry; D010209:Paper; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism; D011138:Polytetrafluoroethylene / Q000737:chemistry*; D040081:Protein Array Analysis / Q000379:methods*; D012997:Solvents / Q000737:chemistry; D013242:Sterilization
chemical_list: D010455:Peptides; D012997:Solvents; D000431:Ethanol; D011138:Polytetrafluoroethylene; D000409:Alanine
keywords: Ligand screening; Paper array; Paper patterning; Peptides; SPOT synthesis