title: Bak Foong pills combined with metformin in the treatment of a polycystic ovarian syndrome rat model.
abstract: The aim of the present study was to investigate the treatment effects and associated mechanism of Bak Foong pills (BFPs) combined with metformin in the treatment of polycystic ovarian syndrome (PCOS). BFPs and/or metformin were administrated to treat the PCOS rats, and the weights and morphologies of the ovary, uterus and adrenal gland were measured. The levels of fasting blood glucose (FBG), serum testosterone (T), luteinizing hormone, fasting insulin (FIN) and insulin-like growth factor-1 were also measured, and the homeostasis model assessment of insulin resistance (HOMA-IR) was calculated. The expression level of androgen receptor (AR) in the ovarian tissue, and the cytochrome P450 cholesterol side-chain cleavage enzyme (P450scc) mRNA levels in the ovary and adrenal tissues were detected. The levels of T, FIN, FBG and HOMA-IR in the combination group were significantly reduced; the wet weights of the ovary and the adrenal gland were decreased significantly, while that of the uterus was increased, and the histological morphology benignly recovered. The rats of each treatment group all experienced restored ovulation. The AR expression level in the treatment group was reduced, and the P450scc mRNA levels in the ovary and the adrenal gland of the combined treatment group were decreased. BFPs combined with metformin significantly affected PCOS, and the possible mechanism involved in the treatment may have been through the reduction of P450scc generation. BFPs may reduce the androgen levels, thus allowing the ovary to restore ovulation.
authors.affiliation: Center of Reproductive Medicine, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, P.R. China.
authors.forename: Wenhui
authors.initials: W
authors.lastname: Liu
authors.affiliation: Translational Medicine Institute, Affiliated to The First People's Hospital of Chenzhou, University of South China, Chenzhou, Hunan 423000, P.R. China.
authors.forename: Wenpei
authors.initials: W
authors.lastname: Liu
authors.affiliation: Department of Obstetrics and Gynecology, The Second Hospital of Yinzhou, Ningbo, Zhejiang 315000, P.R. China.
authors.forename: Yuling
authors.initials: Y
authors.lastname: Fu
authors.affiliation: Center of Reproductive Medicine, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, P.R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Center of Reproductive Medicine, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, P.R. China.
authors.forename: Yuanzhen
authors.initials: Y
authors.lastname: Zhang
keywords: Bak Foong pills; cytochrome P450 cholesterol side-chain cleavage enzyme; insulin-like factor-1; metformin; polycystic ovarian syndrome