title: [Segment Cloning and Expression of Human VIGILIN Gene].
abstract: OBJECTIVE
  To investigate the mechanisms of interaction between high-density lipoprotein binding protein (HDLBP)-VIGILIN with other proteins, we cloned VIGILIN cDNA N, KH1-7, KH8-12, KH13-14, and C fragments separately into expression vector, and identify the expressed proteins.
  
  
  METHODS
  The recombinant plasmid pDsred2-N1/VIGILIN was used as template to amplify VIGILIN full length, VIGILIN N terminal, KH1- 7, KH8-12, KH13-14, C terminal and recombinated them with pGEX 5X 3. After transformed into E. coli BL21 cells, the recombinants were confirmed by enzyme digestion and sequence analysis. After optimizing the IPTG inducing condition, we induced GST-VIGILIN fusion proteins on the appropriate conditions.
  
  
  RESULTS
  The recombinant plasmids of pGEX 5X 3/VIGILIN FL, pGEX 5X 3/VIGILIN N terminal, pGEX 5X 3/VIGILIN KH1-7, pGEX 5X 3/VIGILIN KH8-12, pGEX 5X 3/VIGILIN KH13-14, pGEX 5X 3/VIGILIN C terminal were constructed successfully, and induced the GST-VIGILIN fusion proteins.
  
  
  CONCLUSIONS
  pGEX 5X 3/VIGILIN FL, pGEX 5X 3/VIGILIN N terminal, pGEX 5X 3/VIGILIN KH1-7, pGEX SX 3/VIGILIN KH8-12, pGEX 5X 3/ VIGILIN KH13-14, pGEX 5X 3/VIGILIN C terminal recombinant plasmids were constructed successfully, and their corresponding fusion proteins were successfully expressed.
authors.forename: Xiao-qin
authors.initials: XQ
authors.lastname: Yu
authors.forename: Ling
authors.initials: L
authors.lastname: Wei
authors.forename: Qiu-ying
authors.initials: QY
authors.lastname: Liu
authors.forename: Yuan
authors.initials: Y
authors.lastname: Huang
authors.forename: Rong-ce
authors.initials: RC
authors.lastname: Zhao
authors.forename: Yang
authors.initials: Y
authors.lastname: Qin
mesh_terms: D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004926:Escherichia coli; D006801:Humans; D008075:Lipoproteins, HDL / Q000378:metabolism; D010957:Plasmids; D016601:RNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis
chemical_list: D018076:DNA, Complementary; D008075:Lipoproteins, HDL; D016601:RNA-Binding Proteins; D011993:Recombinant Fusion Proteins; C075197:high density lipoprotein binding protein