title: Comparison of hemostatic dressings for superficial wounds using a new spectrophotometric coagulation assay.
abstract: BACKGROUND
  Due to demographical changes the number of elderly patients depending on oral anticoagulation is expected to rise. Prolonged bleeding times in case of traumatic injuries represent the drawback of these medications, not only in major trauma, but also in superficial wounds. Therefore, dressings capable of accelerating coagulation onset and shortening bleeding times are desirable for these patients.
  
  
  METHODS
  The hemostatic potential and physical properties of different types of superficial wound dressings (standard wound pad, two alginates, chitosan, collagen (Lyostypt(®)), oxidized cellulose, and QuikClot(®)) were assessed in vitro. For this purpose the clotting times of blood under the influence of the named hemostatics from healthy volunteers were compared with Marcumar(®) or ASS(®) treated patients. For that, a newly developed coagulation assay based on spectrophotometric extinction measurements of thrombin activity was used.
  
  
  RESULTS
  The fastest coagulation onset was observed for oxidized cellulose (Ø 2.47 min), Lantor alginate-L (Ø 2.50 min) and QuikClot(®) (Ø 3.01 min). Chitosan (Ø 5.32 min) and the collagen Lyostypt(®) (Ø 7.59 min) induced clotting comparatively late. Regarding physical parameters, QuikClot(®) showed the lowest absorption capacity and speed while chitosan and both alginates achieved the highest. While oxidized cellulose displayed the best clotting times, unfortunately it also revealed low absorption capacity.
  
  
  CONCLUSIONS
  All tested specimens seem to induce clotting independently from the administered type of oral anticoagulant, providing the possibility to neglect the disadvantage in clotting times arising from anticoagulation on a local basis. QuikClot(®), oxidized cellulose and unexpectedly alginate-L were superior to chitosan and Lyostypt(®). Due to its additional well-known positive effect on wound healing alginate-L should be considered for further investigations.
authors.affiliation: Institute for Research in Operative Medicine (IFOM), Witten/Herdecke University, Ostmerheimer Str. 200, 51109, Cologne, Germany. julian-dario.rembe@uni-wh.de.
authors.forename: Julian-Dario
authors.initials: JD
authors.lastname: Rembe
authors.affiliation: Institute for Research in Operative Medicine (IFOM), Witten/Herdecke University, Ostmerheimer Str. 200, 51109, Cologne, Germany. julia.boehm@uni-wh.de.
authors.forename: Julia K
authors.initials: JK
authors.lastname: Böhm
authors.affiliation: Institute for Research in Operative Medicine (IFOM), Witten/Herdecke University, Ostmerheimer Str. 200, 51109, Cologne, Germany. carolin.fromm-dornieden@uni-wh.de.
authors.forename: Carolin
authors.initials: C
authors.lastname: Fromm-Dornieden
authors.affiliation: Institute for Research in Operative Medicine (IFOM), Witten/Herdecke University, Ostmerheimer Str. 200, 51109, Cologne, Germany. nadine.schaefer@uni-wh.de.
authors.forename: Nadine
authors.initials: N
authors.lastname: Schäfer
authors.affiliation: Department of Traumatology, Orthopaedic Surgery and Sports Traumatology, Cologne-Merheim Medical Centre (CMMC), Witten/Herdecke University, Campus Cologne-Merheim, Ostmerheimer Str. 200, 51109, Cologne, Germany. marc.maegele@uni-wh.de.
authors.forename: Marc
authors.initials: M
authors.lastname: Maegele
authors.affiliation: Department of Traumatology, Orthopaedic Surgery and Sports Traumatology, Cologne-Merheim Medical Centre (CMMC), Witten/Herdecke University, Campus Cologne-Merheim, Ostmerheimer Str. 200, 51109, Cologne, Germany. matthias.froehlich@uni-wh.de.
authors.forename: Matthias
authors.initials: M
authors.lastname: Fröhlich
authors.affiliation: Institute for Research in Operative Medicine (IFOM), Witten/Herdecke University, Ostmerheimer Str. 200, 51109, Cologne, Germany. ewa.stuermer@uni-wh.de.
authors.forename: Ewa K
authors.initials: EK
authors.lastname: Stuermer
mesh_terms: D001458:Bandages; D006490:Hemostatics; D006801:Humans; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D006490:Hemostatics