title: Methylation-sensitive amplified polymorphism-based genome-wide analysis of cytosine methylation profiles in Nicotiana tabacum cultivars.
abstract: This study aimed to investigate cytosine methylation profiles in different tobacco (Nicotiana tabacum) cultivars grown in China. Methylation-sensitive amplified polymorphism was used to analyze genome-wide global methylation profiles in four tobacco cultivars (Yunyan 85, NC89, K326, and Yunyan 87). Amplicons with methylated C motifs were cloned by reamplified polymerase chain reaction, sequenced, and analyzed. The results show that geographical location had a greater effect on methylation patterns in the tobacco genome than did sampling time. Analysis of the CG dinucleotide distribution in methylation-sensitive polymorphic restriction fragments suggested that a CpG dinucleotide cluster-enriched area is a possible site of cytosine methylation in the tobacco genome. The sequence alignments of the Nia1 gene (that encodes nitrate reductase) in Yunyan 87 in different regions indicate that a C-T transition might be responsible for the tobacco phenotype. T-C nucleotide replacement might also be responsible for the tobacco phenotype and may be influenced by geographical location.
authors.affiliation: School of Basic Medicine, Xinxiang Medical University, Xinxiang, Henan, China.
authors.forename: J
authors.initials: J
authors.lastname: Jiao
authors.affiliation: School of Basic Medicine, Xinxiang Medical University, Xinxiang, Henan, China.
authors.forename: J
authors.initials: J
authors.lastname: Wu
authors.affiliation: The Third Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan, China.
authors.forename: Z
authors.initials: Z
authors.lastname: Lv
authors.affiliation: Agronomic College, Sichuan Agriculture University, Ya'an, Sichuan, China.
authors.forename: C
authors.initials: C
authors.lastname: Sun
authors.affiliation: School of Basic Medicine, Xinxiang Medical University, Xinxiang, Henan, China.
authors.forename: L
authors.initials: L
authors.lastname: Gao
authors.affiliation: School of Basic Medicine, Xinxiang Medical University, Xinxiang, Henan, China.
authors.forename: X
authors.initials: X
authors.lastname: Yan
authors.affiliation: School of Basic Medicine, Xinxiang Medical University, Xinxiang, Henan, China.
authors.forename: L
authors.initials: L
authors.lastname: Cui
authors.affiliation: Agronomic College, Sichuan Agriculture University, Ya'an, Sichuan, China.
authors.forename: Z
authors.initials: Z
authors.lastname: Tang
authors.affiliation: Agronomic College, Sichuan Agriculture University, Ya'an, Sichuan, China.
authors.forename: B
authors.initials: B
authors.lastname: Yan
authors.affiliation: Pharmacy College, Xinxiang Medical University, Xinxiang, Henan, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Jia
mesh_terms: D002681:China; D018899:CpG Islands / Q000235:genetics; D003596:Cytosine / Q000378:metabolism*; D019175:DNA Methylation / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics; D017343:Genes, Plant / Q000235:genetics; D018745:Genome, Plant / Q000235:genetics; D050901:Nitrate Reductase / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic / Q000235:genetics*; D014026:Nicotiana / Q000235:genetics* / Q000378:metabolism*
chemical_list: D018744:DNA, Plant; D003596:Cytosine; D050901:Nitrate Reductase