title: Determination of the cytotoxic activity of Campylobacter strains isolated from bovine and swine carcasses in north-eastern Poland.
abstract: The study was carried out to determine the cytotoxin production by Campylobacter spp. isolated from slaughtered cattle and swine in north-eastern Poland. In total three commercial slaughterhouses were sampled during one year. Carcass swabs were taken to detect the level of Campylobacter spp. contamination. Campylobacter spp. was found in 50 (34%) out of 147 swine carcasses examined. PCR analysis revealed 4 (8%) isolates to be C. jejuni, and 46 (92%) to be C. coli. From a total of 373 bovine carcasses, Campylobacter spp. were isolated from 49 (13.1%) samples. The results regarding the occurrence of cdt genes associated with cytotoxicity indicated that 100% of C. jejuni and 67.4% C. coli obtained from pigs had all three cdtA, cdtB and cdtC genes. In case of C. jejuni strains isolated from cattle all cdt genes were confirmed in 93.9% isolates. The isolates possessesing all cdt genes had higher cytotoxic activity against cell lines used. The isolates both from cattle and swine were characterized by the highest cytotoxicity against HeLa cells. The values obtained reached 80.8% for C. jejuni isolates from cattle and 76.2% for C. jejuni and 69.0% for C. coli isolates from swine. High prevalence of cytotoxicity in Campylobacter spp. indicates a significant epidemiological role of this pathogen in human infections.
authors.forename: B
authors.initials: B
authors.lastname: Wysok
authors.forename: J
authors.initials: J
authors.lastname: Uradziński
authors.forename: J
authors.initials: J
authors.lastname: Wojtacka
mesh_terms: D000818:Animals; D016466:CHO Cells; D002167:Campylobacter / Q000145:classification / Q000302:isolation & purification* / Q000502:physiology; D002417:Cattle; D002522:Chlorocebus aethiops; D006224:Cricetinae; D003412:Cricetulus; D005516:Food Microbiology; D006367:HeLa Cells; D006801:Humans; D008460:Meat / Q000382:microbiology*; D011044:Poland; D013552:Swine / Q000382:microbiology; D014709:Vero Cells