title: Quantifying the 'escapers' among RNA species.
abstract: tRNAs are fundamental components of translation and emerging evidence places them more centrally in various other cellular processes. However, rather than being uniformly conserved, tRNA abundance is instead highly variable and adaptable. The amount of tRNA genes greatly differs among species. Moreover, even within the same genome, tRNA abundance shapes the proteome in a tissue- and cell-specific manner and is dynamically regulated in response to stress. Here, we review approaches for identification and quantification of tRNAs and their functional integrity. We discuss the resolution of each method and highlight new approaches with cell-wide resolution based on deep-sequencing technologies.
authors.affiliation: Biochemistry and Molecular Biology, Dept. of Chemistry and Biochemistry, University of Hamburg, Hamburg 20146, Germany.
authors.forename: Iolanda
authors.initials: I
authors.lastname: Ferro
authors.affiliation: Biochemistry and Molecular Biology, Dept. of Chemistry and Biochemistry, University of Hamburg, Hamburg 20146, Germany zoya.ignatova@chemie.uni-hamburg.de.
authors.forename: Zoya
authors.initials: Z
authors.lastname: Ignatova
mesh_terms: D000926:Anticodon / Q000235:genetics; D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D009690:Nucleic Acid Conformation; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012313:RNA / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D012343:RNA, Transfer / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
chemical_list: D000926:Anticodon; D012313:RNA; D012343:RNA, Transfer
keywords: ribonucleic acid; transfer ribonucleic acid (tRNA); transfer ribonucleic acid (tRNA) modification; transfer ribonucleic acid (tRNA)-sequencing; translation