title: Investigation of antitumor potential of Ni(II) complexes with tridentate PNO acylhydrazones of 2-(diphenylphosphino)benzaldehyde and monodentate pseudohalides.
abstract: Square-planar azido Ni(II) complex with condensation product of 2-(diphenylphosphino)benzaldehyde and Girard's T reagent was synthesized and its crystal structure was determined. Cytotoxic activity of the azido complex and previously synthesized isothiocyanato, cyanato and chlorido Ni(II) complexes with this ligand was examined on six tumor cell lines (HeLa, A549, K562, MDA-MB-453, MDA-MB-361 and LS-174) and two normal cell line (MRC-5 and BEAS-2B). All the investigated nickel(II) complexes were cytotoxic against all tumor cell lines. The newly synthesized azido complex showed selectivity to HeLa and A549 tumor cell lines compared to the normal cells (for A549 IC50 was similar to that of cisplatin). Azido complex interferes with cell cycle phase distribution of A549 and HeLa cells and possesses nuclease activity towards supercoiled DNA. The observed selectivity of the azido complex for some tumor cell lines can be connected with its strong DNA damaging activity.
authors.affiliation: Faculty of Chemistry, University of Belgrade, Studentski trg 12-16, 11000, Belgrade, Serbia.
authors.forename: Božidar
authors.initials: B
authors.lastname: Čobeljić
authors.affiliation: Faculty of Chemistry, University of Belgrade, Studentski trg 12-16, 11000, Belgrade, Serbia.
authors.forename: Milica
authors.initials: M
authors.lastname: Milenković
authors.affiliation: Faculty of Chemistry and Chemical Technology, University of Ljubljana, Večna pot 113, 1000, Ljubljana, Slovenia.
authors.forename: Andrej
authors.initials: A
authors.lastname: Pevec
authors.affiliation: Faculty of Chemistry and Chemical Technology, University of Ljubljana, Večna pot 113, 1000, Ljubljana, Slovenia.
authors.forename: Iztok
authors.initials: I
authors.lastname: Turel
authors.affiliation: Institute of Chemistry, Technology and Metallurgy, University of Belgrade, Njegoševa 12, P.O. Box 815, 11000, Belgrade, Serbia.
authors.forename: Miroslava
authors.initials: M
authors.lastname: Vujčić
authors.affiliation: Institute of Chemistry, Technology and Metallurgy, University of Belgrade, Njegoševa 12, P.O. Box 815, 11000, Belgrade, Serbia.
authors.forename: Barbara
authors.initials: B
authors.lastname: Janović
authors.affiliation: Laboratory for Experimental Pharmacology, Department of Experimental Oncology, Institute for Oncology and Radiology of Serbia, Pasterova 14, Belgrade, Serbia.
authors.forename: Nevenka
authors.initials: N
authors.lastname: Gligorijević
authors.affiliation: Faculty of Chemistry, University of Belgrade, Studentski trg 12-16, 11000, Belgrade, Serbia.
authors.forename: Dušan
authors.initials: D
authors.lastname: Sladić
authors.affiliation: Laboratory for Experimental Pharmacology, Department of Experimental Oncology, Institute for Oncology and Radiology of Serbia, Pasterova 14, Belgrade, Serbia.
authors.forename: Siniša
authors.initials: S
authors.lastname: Radulović
authors.affiliation: Laboratory for Experimental Pharmacology, Department of Experimental Oncology, Institute for Oncology and Radiology of Serbia, Pasterova 14, Belgrade, Serbia.
authors.forename: Katarina
authors.initials: K
authors.lastname: Jovanović
authors.affiliation: Faculty of Chemistry, University of Belgrade, Studentski trg 12-16, 11000, Belgrade, Serbia. kka@chem.bg.ac.rs.
authors.forename: Katarina
authors.initials: K
authors.lastname: Anđelković
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D001547:Benzaldehydes / Q000737:chemistry*; D018360:Crystallography, X-Ray; D006219:Halogens / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D006835:Hydrazones / Q000737:chemistry*; D008856:Microscopy, Fluorescence; D009532:Nickel / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000970:Antineoplastic Agents; D001547:Benzaldehydes; D006219:Halogens; D006835:Hydrazones; D009532:Nickel
keywords: Cytotoxicity; DNA interactions; Girard’s T reagent; Nickel(II) complexes; Phosphine ligands