title: Pseudotyping of G-Gene-Deficient Rabies Virus.
abstract: G-deleted fluorescent rabies virus (RV) pseudotyped with RV G proteins, SAD ΔG eGFP (RV CVS-G), can be used as single-round vectors for efficient retrograde labeling of neurons. For these experiments, as well as for monosynaptic tracing, which involves pseudotyping in situ, the use of the CVS strain G is recommended because of its high tropism for neurons. Pseudotype virus stocks generated by transfection of pCAGGS-G (or in MG139-on cells) contain the G protein of the vaccine strain SAD L16, which is broader in its tropism, and infects astrocytes, glia, and oligodendrocytes. We also describe a procedure for pseudotyping with ASLV Env A, which uses a cell-line expressing a version of the EnvA protein that is incorporated efficiently into the RV envelope (EnvARG(RGct)).
authors.forename: Nadin
authors.initials: N
authors.lastname: Hagendorf
authors.forename: Karl-Klaus
authors.initials: KK
authors.lastname: Conzelmann
mesh_terms: D000956:Antigens, Viral / Q000378:metabolism*; D002460:Cell Line; D017353:Gene Deletion; D017930:Genes, Reporter; D006023:Glycoproteins / Q000172:deficiency / Q000378:metabolism*; D006801:Humans; D008164:Luminescent Proteins / Q000032:analysis / Q000235:genetics; D009474:Neurons / Q000737:chemistry / Q000821:virology*; D061848:Optical Imaging / Q000379:methods; D011820:Rabies virus / Q000235:genetics*; D011994:Recombinant Proteins / Q000032:analysis / Q000235:genetics; D013194:Staining and Labeling / Q000379:methods; D013569:Synapses / Q000737:chemistry / Q000821:virology*; D014759:Viral Envelope Proteins / Q000172:deficiency / Q000378:metabolism*
chemical_list: D000956:Antigens, Viral; D006023:Glycoproteins; D008164:Luminescent Proteins; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins; C064951:glycoprotein G, Rabies virus