title: Ursolic Acid Inhibits the Proliferation of Gastric Cancer Cells by Targeting miR-133a.
abstract: Ursolic acid (UA), a potential chemotherapeutic agent, has the properties of inhibition of the growth of many human cancer cell lines. Whether UA can inhibit the growth and metastasis of human gastric cancer cells remains unknown. In this study, it was found that UA inhibited the growth and metastasis of human gastric cancer cells in vitro. Our results showed the increase of the percent of apoptotic cells and G1 phase, the inhibition of cell migrations well as the decrease of the expression of Bax, caspase 3 and Bcl-2 in BGC-823 cells after the treatment with UA. Real-time quantitative PCR analysis showed that UA treatment upregulated the level of miR-133a in BGC-823 cells. Overexpression of miR-133a increased the G1 phase of cell cycle and decreased Akt1 expression in BGC-823 cells. These outcomes might be secondary to the increased expression of miR-133a after the treatment with UA.
authors.affiliation: Department of Laboratory Medicine, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, P.R. China.
authors.forename: Fenfen
authors.initials: F
authors.lastname: Xiang
authors.forename: Chunying
authors.initials: C
authors.lastname: Pan
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Kong
authors.forename: Rong
authors.initials: R
authors.lastname: Wu
authors.forename: Jiemin
authors.initials: J
authors.lastname: Jiang
authors.forename: Yueping
authors.initials: Y
authors.lastname: Zhan
authors.forename: Jian
authors.initials: J
authors.lastname: Xu
authors.forename: Xingang
authors.initials: X
authors.lastname: Gu
authors.forename: Xiangdong
authors.initials: X
authors.lastname: Kang
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D016503:Drug Delivery Systems / Q000379:methods*; D006801:Humans; D035683:MicroRNAs / Q000819:agonists / Q000096:biosynthesis* / Q000235:genetics; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000517:prevention & control; D014315:Triterpenes / Q000008:administration & dosage*; D000097245:Ursolic Acid
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D035683:MicroRNAs; D014315:Triterpenes; D000097245:Ursolic Acid; C548234:MIRN133 microRNA, human