title: Parallel Reaction Monitoring: A Targeted Experiment Performed Using High Resolution and High Mass Accuracy Mass Spectrometry.
abstract: The parallel reaction monitoring (PRM) assay has emerged as an alternative method of targeted quantification. The PRM assay is performed in a high resolution and high mass accuracy mode on a mass spectrometer. This review presents the features that make PRM a highly specific and selective method for targeted quantification using quadrupole-Orbitrap hybrid instruments. In addition, this review discusses the label-based and label-free methods of quantification that can be performed with the targeted approach.
authors.affiliation: W.M. Keck Foundation Biotechnology Resource Laboratory, School of Medicine, Yale University, 300 George Street, New Haven, CT 06511, USA. navin.rauniyar@yale.edu.|Department of Molecular Biophysics &amp; Biochemistry, Yale University, New Haven, CT 06520, USA. navin.rauniyar@yale.edu.
authors.forename: Navin
authors.initials: N
authors.lastname: Rauniyar
mesh_terms: D000818:Animals; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods* / Q000592:standards; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D011506:Proteins / Q000737:chemistry / Q000378:metabolism; D040901:Proteomics / Q000379:methods* / Q000592:standards
chemical_list: D010455:Peptides; D011506:Proteins
keywords: PRM; Q-Exactive; SRM; accurate mass; high resolution; mass spectrometry; parallel reaction monitoring; proteomics; quantitative proteomics; selected reaction monitoring; targeted