title: Association of long non-coding RNA HOTTIP with progression and prognosis in colorectal cancer.
abstract: Long non-coding RNA (lncRNA) has an important role in carcinoma progression and prognosis. However, little is known about the pathological role of lncRNA HOTTIP (HOXA transcript at the distal tip) in colorectal cancer (CRC) patients. This study attempted to investigate the association of lncRNA HOTTIP expression with progression and prognosis in CRC patients. LncRNA HOTTIP expression was measured in 156 CRC tissues and 21 adjacent non-malignant tissues using qRT-PCR. In present study, our results indicated that lncRNA HOTTIP was highly expressed in CRC compared with adjacent non-malignant tissues (P<0.001), and positively correlated with T stage (T1-2 vs. T3-4, P = 0.001), clinical stage (I-II stages vs. III-IV stages, P = 0.003), and distant metastasis (absent vs. present, P = 0.014) in CRC patients. Furthermore, we also observed that increased lncRNA HOTTIP expression was an unfavorable prognostic factor in CRC patients (P = 0.001), regardless of T stage, distant metastasis and clinical stage. Finally, overexpression of lncRNA HOTTIP was supposed to be an independent poor prognostic factor for CRC patients through multivariate analysis (P = 0.017). In conclusion, lncRNA HOTTIP overexpression maybe serves as an unfavorable prognosis predictor for CRC patients. However, a further larger sample size investigation is needed to support our results.
authors.affiliation: Department of General Surgery, Affiliated Cancer Hospital of Zhengzhou University China.
authors.forename: Ying-Kun
authors.initials: YK
authors.lastname: Ren
authors.affiliation: Department of Gynecologic Oncology, Affiliated Cancer Hospital of Zhengzhou University China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Xiao
authors.affiliation: Department of General Surgery, Affiliated Cancer Hospital of Zhengzhou University China.
authors.forename: Xiang-Bin
authors.initials: XB
authors.lastname: Wan
authors.affiliation: Department of General Surgery, Affiliated Cancer Hospital of Zhengzhou University China.
authors.forename: Yu-Zhou
authors.initials: YZ
authors.lastname: Zhao
authors.affiliation: Department of General Surgery, Affiliated Cancer Hospital of Zhengzhou University China.
authors.forename: Jian
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Thoracic Surgery, Affiliated Cancer Hospital of Zhengzhou University China.
authors.forename: Yin
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of General Surgery, Affiliated Cancer Hospital of Zhengzhou University China.
authors.forename: Guang-Sen
authors.initials: GS
authors.lastname: Han
authors.affiliation: Department of Oncology, Affiliated Cancer Hospital of Zhengzhou University China.
authors.forename: Xiao-Bing
authors.initials: XB
authors.lastname: Chen
authors.affiliation: Department of General Surgery, Affiliated Cancer Hospital of Zhengzhou University China.
authors.forename: Qi-Yun
authors.initials: QY
authors.lastname: Zou
authors.affiliation: Department of General Surgery, Affiliated Cancer Hospital of Zhengzhou University China.
authors.forename: Gang-Cheng
authors.initials: GC
authors.lastname: Wang
authors.affiliation: Department of General Surgery, Affiliated Cancer Hospital of Zhengzhou University China.
authors.forename: Chao-Min
authors.initials: CM
authors.lastname: Lu
authors.affiliation: Department of General Surgery, Affiliated Cancer Hospital of Zhengzhou University China.
authors.forename: Yong-Chao
authors.initials: YC
authors.lastname: Xu
authors.affiliation: Department of General Surgery, Affiliated Cancer Hospital of Zhengzhou University China.
authors.forename: You-Cai
authors.initials: YC
authors.lastname: Wang
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000032:analysis / Q000235:genetics*; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D018450:Disease Progression; D005260:Female; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D016016:Proportional Hazards Models; D062085:RNA, Long Noncoding / Q000032:analysis / Q000096:biosynthesis*; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D014408:Biomarkers, Tumor; D062085:RNA, Long Noncoding
keywords: HOTTIP; colorectal cancer; lncRNA; prognosis