title: Anti-skeletal muscle atrophy effect of Oenothera odorata root extract via reactive oxygen species-dependent signaling pathways in cellular and mouse model.
abstract: Skeletal muscle atrophy can be defined as a decrease of muscle volume caused by injury or lack of use. This condition is associated with reactive oxygen species (ROS), resulting in various muscular disorders. We acquired 2D and 3D images using micro-computed tomography in gastrocnemius and soleus muscles of sciatic-denervated mice. We confirmed that sciatic denervation-small animal model reduced muscle volume. However, the intraperitoneal injection of Oenothera odorata root extract (EVP) delayed muscle atrophy compared to a control group. We also investigated the mechanism of muscle atrophy's relationship with ROS. EVP suppressed expression of SOD1, and increased expression of HSP70, in both H2O2-treated C2C12 myoblasts and sciatic-denervated mice. Moreover, EVP regulated apoptotic signals, including caspase-3, Bax, Bcl-2, and ceramide. These results indicate that EVP has a positive effect on reducing the effect of ROS on muscle atrophy.
authors.affiliation: a Division of Biological Science and Technology, Yonsei-Fraunhofer Medical Device Lab , College of Science and Technology, Yonsei University , Wonju , Korea.
authors.forename: Yong-Hyeon
authors.initials: YH
authors.lastname: Lee
authors.affiliation: a Division of Biological Science and Technology, Yonsei-Fraunhofer Medical Device Lab , College of Science and Technology, Yonsei University , Wonju , Korea.
authors.forename: Wan-Joong
authors.initials: WJ
authors.lastname: Kim
authors.affiliation: a Division of Biological Science and Technology, Yonsei-Fraunhofer Medical Device Lab , College of Science and Technology, Yonsei University , Wonju , Korea.
authors.forename: Myung-Hun
authors.initials: MH
authors.lastname: Lee
authors.affiliation: a Division of Biological Science and Technology, Yonsei-Fraunhofer Medical Device Lab , College of Science and Technology, Yonsei University , Wonju , Korea.
authors.forename: Sun-Young
authors.initials: SY
authors.lastname: Kim
authors.affiliation: b Department of Biomedical Engineering, Yonsei-Fraunhofer Medical Device Lab , College of Health Science, Yonsei University , Wonju , Korea.
authors.forename: Dong-Hyun
authors.initials: DH
authors.lastname: Seo
authors.affiliation: b Department of Biomedical Engineering, Yonsei-Fraunhofer Medical Device Lab , College of Health Science, Yonsei University , Wonju , Korea.
authors.forename: Han-Sung
authors.initials: HS
authors.lastname: Kim
authors.affiliation: c Center for Translational Bone, Joint and Soft Tissue Research, Medical Faculty and University Hospital, Technische Universität Dresden , Dresden , Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Gelinsky
authors.affiliation: a Division of Biological Science and Technology, Yonsei-Fraunhofer Medical Device Lab , College of Science and Technology, Yonsei University , Wonju , Korea.
authors.forename: Tack-Joong
authors.initials: TJ
authors.lastname: Kim
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D002518:Ceramides / Q000378:metabolism; D003714:Denervation / Q000009:adverse effects; D004195:Disease Models, Animal; D005786:Gene Expression Regulation; D018840:HSP70 Heat-Shock Proteins / Q000819:agonists / Q000235:genetics / Q000378:metabolism; D007274:Injections, Intraperitoneal; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D009133:Muscular Atrophy / Q000188:drug therapy* / Q000209:etiology / Q000378:metabolism / Q000473:pathology; D032446:Myoblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D031664:Oenothera / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors* / Q000378:metabolism; D012584:Sciatic Nerve / Q000601:surgery; D015398:Signal Transduction / Q000187:drug effects*; D013482:Superoxide Dismutase / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D000072105:Superoxide Dismutase-1; D051028:bcl-2-Associated X Protein / Q000235:genetics / Q000378:metabolism
chemical_list: C494827:Bax protein, mouse; D002518:Ceramides; D018840:HSP70 Heat-Shock Proteins; D010936:Plant Extracts; D019253:Proto-Oncogene Proteins c-bcl-2; D017382:Reactive Oxygen Species; D051028:bcl-2-Associated X Protein; C052617:Bcl2 protein, mouse; C000606291:Sod1 protein, mouse; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1; C505409:Casp3 protein, mouse; D053148:Caspase 3
keywords: Oenothera odorata; heat shock protein 70; muscle atrophy; reactive oxygen species; superoxide dismutase