title: Ahrensia marinasp. nov., a dimethylsulfoniopropionate-cleaving bacterium isolated from seawater, and emended descriptions of the genusAhrensiaandAhrensia kielensis.
abstract: A Gram-stain-negative, strictly aerobic, light-beige, rod-shaped, motile bacterium with peritrichous flagella that cleaves dimethylsulfoniopropionate (DMSP), designated strain LZD062T, was isolated from bottom seawater of the East China Sea. The isolate required sea salts for growth and grew optimally at pH 8.0 and 28 °C and in the presence of 2 % (w/v) NaCl. The major fatty acid (>10 %) was summed feature 8 (C18 : 1ω7cand/or C18 : 1ω6c) and the major polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, one unidentified aminolipid and one unidentified glycolipid. Ubiquinone Q-10 was the only quinone detected. Phylogenetic analyses based on 16S rRNA andatpDgene sequences placed LZD062Twithin the genusAhrensiaof the familyPhyllobacteriaceaein the classAlphaproteobacteria. The most closely related type strain was, in both cases,Ahrensia kielensisJCM 20689T( = IAM 12618T= DSM 5890T), which gave sequence similarities of 97.7 % in the 16S rRNA gene and 90.4 % in theatpDgene. Genome relatedness between strain LZD062TandA. kielensisJCM 20689Twas computed using both genome-to-genome distance analysis and average nucleotide identity, giving values of 22.10 ± 2.35 and 79.55 %, respectively. The genomic DNA G+C content calculated from the genome sequence was 50.1 mol%. On the basis of our polyphasic analyses, strain LZD062Tis considered to represent a novel species of the genusAhrensia, for which the nameAhrensia marinasp. nov. is proposed. The type strain is LZD062T( = MCCC 1K00254T= JCM 30117T= DSM 28886T). Emended descriptions of the genusAhrensiaandAhrensia kielensisare also proposed.
authors.affiliation: College of Marine Life Sciences, Ocean University of China, Qingdao, PR China.
authors.forename: Ji
authors.initials: J
authors.lastname: Liu
authors.affiliation: College of Marine Life Sciences, Ocean University of China, Qingdao, PR China.
authors.forename: Yanan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Marine Life Sciences, Ocean University of China, Qingdao, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Institute of Evolution & Marine Biodiversity, Ocean University of China, Qingdao 266003, PR China.|College of Marine Life Sciences, Ocean University of China, Qingdao, PR China.
authors.forename: Xiao-Hua
authors.initials: XH
authors.lastname: Zhang