title: Differential Analysis of 2-D Maps by Pixel-Based Approaches.
abstract: Two approaches to the analysis of 2-D maps are available: the first one involves a step of spot detection on each gel image; the second one is based instead on the direct differential analysis of 2-D map images, following a pixel-based procedure. Both approaches strongly depend on the proper alignment of the gel images, but the pixel-based approach allows to solve important drawbacks of the spot-volume procedure, i.e., the problem of missing data and of overlapping spots. However, this approach is quite computationally intensive and requires the use of algorithms able to separate the information (i.e., spot-related information) from the background. Here, the most recent pixel-based approaches are described.
authors.affiliation: Department of Sciences and Technological Innovation, University of Piemonte Orientale, Viale Michel 11, 15121, Alessandria, Italy. emilio.marengo@uniupo.it.
authors.forename: Emilio
authors.initials: E
authors.lastname: Marengo
authors.affiliation: Department of Sciences and Technological Innovation, University of Piemonte Orientale, Viale Michel 11, 15121, Alessandria, Italy. elisa.robotti@uniupo.it.
authors.forename: Elisa
authors.initials: E
authors.lastname: Robotti
authors.affiliation: Department of Sciences and Technological Innovation, University of Piemonte Orientale, Viale Michel 11, 15121, Alessandria, Italy.
authors.forename: Fabio
authors.initials: F
authors.lastname: Quasso
mesh_terms: D000465:Algorithms; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D011506:Proteins
keywords: Fuzzy logic; Gel-electrophoresis; Pixel-based approach; Three-way PCA