title: Ochrobactrum endophyticum sp. nov., isolated from roots of Glycyrrhiza uralensis.
abstract: A novel Gram-staining negative, motile, rod-shaped and aerobic bacterial strain, designated EGI 60010(T), was isolated from healthy roots of Glycyrrhiza uralensis F. collected from Yili County, Xinjiang Province, North-West China. The 16S rRNA gene sequence of strain EGI 60010(T) showed 97.2 % sequence similarities with Ochrobactrum anthropi ATCC 49188(T) and Ochrobactrum cytisi ESC1(T), and 97.1 % with Ochrobactrum lupini LUP21(T). The phylogenetic analysis based on 16S rRNA gene sequences showed that the new isolate clustered with members of the genera Ochrobactrum, and formed a distinct clade in the neighbour-joining tree. Q-10 was identified as the respiratory quinone for strain EGI 60010(T). The major fatty acids were summed feature 8 (C18:1 ω6c and/or C18:1 ω7c), C19:0 cyclo ω8c, summed feature 4 (C17:1 iso I/anteiso B) and C16:0. The polar lipids detected were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylglycerol and phosphatidylcholine. The DNA G+C content of strain EGI 60010(T) was determined to be 60.4 mol%. The genomic DNA relatedness values determined between strain EGI 60010(T) and the closely related strains O. anthropi JCM 21032(T), O. cytisi CCTCC AB2014258(T) and O. lupini NBRC 102587(T) were 50.3, 50.0 and 41.6 %, respectively. Based on the results of the molecular studies supported by its differentiating phenotypic characteristics, strain EGI 60010(T) was considered to represent a novel species within the genus Ochrobactrum, for which the name Ochrobactrum endophyticum sp. nov., is proposed. The type strain is EGI 60010(T) (=CGMCC 1.15082(T) = KCTC 42485(T) = DSM 29930(T)).
authors.affiliation: Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürümqi, 830011, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: Kunming Medical University Haiyuan College, Kunming, 650106, People's Republic of China.
authors.forename: Yan-Qiong
authors.initials: YQ
authors.lastname: Li
authors.affiliation: Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, People's Republic of China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Jiang
authors.affiliation: China Tobacco Yunnan Industrial Co. Ltd., Kunming, 650231, People's Republic of China.
authors.forename: Rui
authors.initials: R
authors.lastname: Gao
authors.affiliation: State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, College of Ecology and Evolution, Sun Yat-Sen University, Guangzhou, 510275, People's Republic of China.
authors.forename: Salam
authors.initials: S
authors.lastname: Nimaichand
authors.affiliation: China Tobacco Yunnan Industrial Co. Ltd., Kunming, 650231, People's Republic of China.
authors.forename: Yan-Qing
authors.initials: YQ
authors.lastname: Duan
authors.affiliation: Department of Biotechnology and Microbiology, Faculty of Biology and Soil Science, National University of Uzbekistan, Tashkent, Republic of Uzbekistan, 100174.
authors.forename: Dilfuza
authors.initials: D
authors.lastname: Egamberdieva
authors.affiliation: China Tobacco Yunnan Industrial Co. Ltd., Kunming, 650231, People's Republic of China. kibchenwei@126.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.affiliation: Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürümqi, 830011, People's Republic of China. liwenjun3@mail.sysu.edu.cn.|Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, People's Republic of China. liwenjun3@mail.sysu.edu.cn.|State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, College of Ecology and Evolution, Sun Yat-Sen University, Guangzhou, 510275, People's Republic of China. liwenjun3@mail.sysu.edu.cn.
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Li
mesh_terms: D001482:Base Composition; D002681:China; D005227:Fatty Acids / Q000032:analysis; D029882:Glycyrrhiza uralensis / Q000382:microbiology*; D042041:Ochrobactrum / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology*; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D005227:Fatty Acids; D010743:Phospholipids; D011809:Quinones; D012336:RNA, Ribosomal, 16S
keywords: Endophytic bacteria; Glycyrrhiza uralensis F.; Ochrobactrum endophyticum sp. nov.