title: Overexpression of AtPTPA in Arabidopsis increases protein phosphatase 2A activity by promoting holoenzyme formation and ABA negatively affects holoenzyme formation.
abstract: AtPTPA is a critical regulator for the holoenzyme assembling of protein phosphatase 2A (PP2A) in Arabidopsis. Characterization of AtPTPA improves our understanding of the function and regulation of PP2A in eukaryotes. Further analysis of AtPTPA-overexpressing plants indicates that AtPTPA increases PP2A activity by promoting PP2A's AC dimer formation, thereby holoenzyme assembling. Plant hormone abscisic acid (ABA) reduces PP2A enzyme activity by negatively affects PP2A's AC dimer formation. Therefore, AtPTPA is a positive factor that promotes PP2A holoenzyme assembly, and ABA is a negative factor that prevents PP2A holoenzyme assembly.
authors.affiliation: a Department of Biological Sciences ; Texas Tech University ; Lubbock , TX USA.
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
authors.affiliation: a Department of Biological Sciences ; Texas Tech University ; Lubbock , TX USA.
authors.forename: Xunlu
authors.initials: X
authors.lastname: Zhu
authors.affiliation: b Zhejiang Academy of Agricultural Sciences ; Hangzhou, Zhejiang Province , China.
authors.forename: Guoxin
authors.initials: G
authors.lastname: Shen
authors.affiliation: a Department of Biological Sciences ; Texas Tech University ; Lubbock , TX USA.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology*; D017360:Arabidopsis / Q000187:drug effects / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D020035:Holoenzymes / Q000378:metabolism*; D018832:Molecular Chaperones / Q000378:metabolism*; D054648:Protein Phosphatase 2 / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D020035:Holoenzymes; D018832:Molecular Chaperones; C000610671:PTPA protein, Arabidopsis; D000040:Abscisic Acid; D054648:Protein Phosphatase 2
keywords: Arabidopsis; PP2A holoenzyme assembly; PP2Ac methylation; protein phosphatase 2A