title: Expression and mutation analysis of Cyclin A and Ki-67 in glioma and their correlation with tumor progression.
abstract: The present study aimed to analyze the expression and mutation of Cyclin A and Ki-67 in gliomas, and determine their correlation with tumor progression. Tissue samples of 186 diagnosed glioma patients were examined immunohistochemically for Cyclin A and Ki-67 expression. Gene mutation analysis was performed on genomic DNA extracted from patient samples, using polymerase chain reaction amplification and sequencing. Cyclin A and Ki-67 expression were observed in the glioma and lymphatic metastasis tissues, and were analyzed using SPSS 14.0 statistical software. Of the total patients, 64 (34.41%) were Cyclin A-positive and 68 (36.56%) were Ki-67-positive. The expression of Cyclin A and Ki-67 in glioma was positively correlated with lymphatic metastasis. Statistically significant differences were observed in the mutation rate of Ki-67 (P<0.05), but not Cyclin A (P>0.05), between the gliomas and metastatic tumors. In conclusion, Cyclin A and Ki-67 are highly expressed in glioma tissues, and their expression and mutation are associated with the lymphatic metastasis of glioma in the brain. It may be concluded that Cyclin A and Ki-67 may be used as biomarkers to guide the diagnosis of glioma and evaluate the prognosis of affected patients.
authors.affiliation: Department of Neurology, Xinxiang Central Hospital, Xinxiang, Henan 453000, P.R. China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Miao
authors.affiliation: Department of Anatomy, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China.
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Neurology, Xinxiang Central Hospital, Xinxiang, Henan 453000, P.R. China.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Neurology, Xinxiang Central Hospital, Xinxiang, Henan 453000, P.R. China.
authors.forename: Xunzhao
authors.initials: X
authors.lastname: Gao
keywords: Cyclin A; Ki-67; glioma; lymphatic metastasis