title: The circulating microRNome demonstrates distinct lymphocyte subset-dependent signatures.
abstract: Upon activation, lymphocytes release vesicles containing microRNAs (miRNAs). However, little is known as to whether this release results in modulation of circulating miRNAs (the miRNome) in the serum. The present work aims to identify lymphocyte subset-specific signatures of miRNAs within the serum circulating miRNome. We therefore assessed serum miRNA expression profiles in wild-type mice; in mice lacking either CD4(+) T cells, CD8(+) T cells, invariant natural killer T (iNKT) cells, or B cells; and, as a control, in mice in which Dicer has been ablated in T lymphocytes. We found that specific serum miRNAs are differentially modulated when different lymphocyte subsets are lacking. In particular, the serum level of miR-181b-5p, previously demonstrated to be fundamental for the development of iNKT cells, is specifically reduced in mice in which iNKT cells are absent. Interestingly, our results indicate a direct link between the biological role of a single miRNA in lymphocyte development and its serum level, and prove that even a population composed of relatively few cells in vivo, such as iNKT lymphocytes, has a measurable effect on the serum circulating miRNome.
authors.affiliation: Istituto Nazionale Genetica Molecolare "Romeo ed Enrica Invernizzi", Milan, Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: de Candia
authors.affiliation: Istituto Nazionale Genetica Molecolare "Romeo ed Enrica Invernizzi", Milan, Italy.
authors.forename: Anna
authors.initials: A
authors.lastname: Torri
authors.affiliation: Experimental Immunology Unit, San Raffaele Scientific Institute, Milan, Italy.
authors.forename: Maya
authors.initials: M
authors.lastname: Fedeli
authors.affiliation: Experimental Immunology Unit, San Raffaele Scientific Institute, Milan, Italy.
authors.forename: Valentina
authors.initials: V
authors.lastname: Viganò
authors.affiliation: Istituto Nazionale Genetica Molecolare "Romeo ed Enrica Invernizzi", Milan, Italy.
authors.forename: Donatella
authors.initials: D
authors.lastname: Carpi
authors.affiliation: Istituto Nazionale Genetica Molecolare "Romeo ed Enrica Invernizzi", Milan, Italy.
authors.forename: Tatiana
authors.initials: T
authors.lastname: Gorletta
authors.affiliation: Experimental Immunology Unit, San Raffaele Scientific Institute, Milan, Italy.
authors.forename: Giulia
authors.initials: G
authors.lastname: Casorati
authors.affiliation: Istituto Nazionale Genetica Molecolare "Romeo ed Enrica Invernizzi", Milan, Italy.|Department of Medical Biotechnology and Translational Medicine, University of Milan, Milan, Italy.
authors.forename: Massimiliano
authors.initials: M
authors.lastname: Pagani
authors.affiliation: Experimental Immunology Unit, San Raffaele Scientific Institute, Milan, Italy.
authors.forename: Paolo
authors.initials: P
authors.lastname: Dellabona
authors.affiliation: Istituto Nazionale Genetica Molecolare "Romeo ed Enrica Invernizzi", Milan, Italy.|DISCCO, Department of Clinical Sciences and Community Health, University of Milan, Milan, Italy.
authors.forename: Sergio
authors.initials: S
authors.lastname: Abrignani
mesh_terms: D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D016207:Cytokines / Q000096:biosynthesis; D053487:DEAD-box RNA Helicases / Q000172:deficiency / Q000235:genetics; D005434:Flow Cytometry; D051379:Mice; D035683:MicroRNAs / Q000097:blood* / Q000235:genetics; D055611:Natural Killer T-Cells / Q000276:immunology*; D043244:Ribonuclease III / Q000172:deficiency / Q000235:genetics; D016176:T-Lymphocyte Subsets / Q000276:immunology*
chemical_list: D016207:Cytokines; D035683:MicroRNAs; C509781:Dicer1 protein, mouse; D043244:Ribonuclease III; D053487:DEAD-box RNA Helicases
keywords: Circulating microRNAs · invariant natural killer T lymphocytes · Lymphocyte subsets