title: Distribution of HPV genotypes in Shanghai women.
abstract: OBJECTIVE
  To study the Distribution of HPV genotypes in Shanghai women.
  
  
  METHODS
  Cohort study.
  
  
  METHODS
  Shanghai First Maternity and Infant Hospital affiliated with Tongji University.
  
  
  METHODS
  Patients those attended in the cervical disease diagnosis and treatment center of Shanghai First Maternity and Infant Hospital between January 2011 and December 2014.
  
  
  METHODS
  HPV GenoArray test kit (HybriBio Ltd) was used to perform HPV genotyping and was also used in DNA amplification and HybriBio's proprietary flow-through hybridization technique.
  
  
  RESULTS
  In this study, total patients analyzed were 4585. Among 4585 sample the HPV positive patients were 1460 i.e. 31.84% in total. On the basis of pathological report normal were 1358, with inflammation 2441, with low grade lesion were 399, high grade lesion were 353, CIN were 19 and cervical carcinoma were 15. Among normal HPV positive were 215 (15.8%), among inflammation HPV positive were 735 (30.11%). HPV positive in low grade lesion were 353 i.e. 59.77%. In high grade lesion 211 were HPV positive among 272 (68.17%). The percentage of HPV positive was 73.68% i.e. 14 out of 19 patient in cervical carcinoma in situ. 13 patient out of 15 i.e. 86.67% of Cervical carcinoma were HPV positive. Among all percentage of HPV positive was high among cervical carcinoma then cervical carcinoma in situ then high grade lesion in decreasing fashion to low grade lesion and in normal. Highest prevalence i.e. 22.67% is of HPV 52 subtype and HPV 16 has second highest prevalence with 17.67% among HPV positive cases. Sensitivity of TCT detection is 71.6%. Specificity of TCT detection is 79.6%. Sensitivity of HPV-DNA detection is 65.2%. Specificity of HPV-DNA detection is 78.2%.
  
  
  CONCLUSIONS
  HPV is one of major health concern in shanghai having high prevalence rate in comparison to other part of china and other part of world. This has implications for the future cervical cancer burden and the priority to be given to prevent cervical cancer in Shanghai, especially, given the promising efficacy of prophylactic vaccines against HPV52, 16 and 58. This study also shows high sensitivity and specificity of TCT and HPV-DNA detection.
authors.affiliation: Department of Gynecology, Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine Shanghai 200040, China.
authors.forename: Suman
authors.initials: S
authors.lastname: Singh
authors.affiliation: Department of Gynecology, Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine Shanghai 200040, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhou
authors.affiliation: Department of Gynecology, Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine Shanghai 200040, China.
authors.forename: Yunyun
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Department of Gynecology, Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine Shanghai 200040, China.
authors.forename: Xianghong
authors.initials: X
authors.lastname: Xu
authors.affiliation: Department of Gynecology, Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine Shanghai 200040, China.
authors.forename: Xiaojie
authors.initials: X
authors.lastname: Huang
authors.affiliation: Department of Gynecology, Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine Shanghai 200040, China.
authors.forename: Junwei
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Department of Gynecology, Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine Shanghai 200040, China.
authors.forename: Lingfei
authors.initials: L
authors.lastname: Han
authors.affiliation: Department of Gynecology, Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine Shanghai 200040, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Wang
authors.affiliation: Department of Gynecology, Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine Shanghai 200040, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Sun
authors.affiliation: Department of Gynecology, Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine Shanghai 200040, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Li
mesh_terms: D000293:Adolescent; D000328:Adult; D002277:Carcinoma / Q000175:diagnosis / Q000453:epidemiology / Q000821:virology*; D002681:China / Q000453:epidemiology; D004279:DNA, Viral / Q000235:genetics*; D005260:Female; D005838:Genotype; D061809:Human Papillomavirus DNA Tests; D006801:Humans; D008875:Middle Aged; D017720:Molecular Epidemiology; D027383:Papillomaviridae / Q000235:genetics* / Q000302:isolation & purification; D030361:Papillomavirus Infections / Q000175:diagnosis / Q000453:epidemiology / Q000821:virology*; D011237:Predictive Value of Tests; D015995:Prevalence; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014504:Urban Health; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis / Q000453:epidemiology / Q000821:virology*; D055815:Young Adult; D002578:Uterine Cervical Dysplasia / Q000175:diagnosis / Q000453:epidemiology / Q000821:virology*
chemical_list: D004279:DNA, Viral
keywords: Cervical intraepithelial neoplasia; HPV distribution in shanghai; HPV genotype; HPV infection; HPV subtypes; TCT sensitivity; TCT specificity