title: LncRNA NONRATT021972 involved the pathophysiologic processes mediated by P2X7 receptors in stellate ganglia after myocardial ischemic injury.
abstract: Adenosine triphosphate (ATP) acts on P2X receptors to initiate signal transmission. P2X7 receptors play a role in the pathophysiological process of myocardial ischemic injury. Long noncoding RNAs (lncRNAs) participate in numerous biological functions independent of protein translation. LncRNAs are implicated in nervous system diseases. This study investigated the effects of NONRATT021972 small interference RNA (siRNA) on the pathophysiologic processes mediated by P2X7 receptors in stellate ganglia (SG) after myocardial ischemic injury. Our results demonstrated that the expression of NONRATT021972 in SG was significantly higher in the myocardial ischemic (MI) group than in the control group. Treatment of MI rats with NONRATT021972 siRNA, the P2X7 antagonist brilliant blue G (BBG), or P2X7 siRNA improved the histology of injured ischemic cardiac tissues and decreased the elevated concentrations of serum myocardial enzymes, creatine kinase (CK), CK isoform MB (CK-MB), lactate dehydrogenase (LDH) and aspartate aminotransferase (AST) compared to the MI rats. NONRATT021972 siRNA, BBG, or P2X7 siRNA treatment in MI rats decreased the expression levels of P2X7 immunoreactivity, P2X7 messenger RNA (mRNA), and P2X7 protein, interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and phosphorylated p38 mitogen-activated protein kinase (p38 MAPK) in the SG compared to MI rats. NONRATT021972 siRNA treatment prevented the pathophysiologic processes mediated by P2X7 receptors in the SG after myocardial ischemic injury.
authors.affiliation: Department of Physiology, Medical School of Nanchang University, Nanchang, 330006, People's Republic of China.
authors.forename: Lifang
authors.initials: L
authors.lastname: Zou
authors.affiliation: Department of Physiology, Medical School of Nanchang University, Nanchang, 330006, People's Republic of China.
authors.forename: Guihua
authors.initials: G
authors.lastname: Tu
authors.affiliation: Undergraduate student of grade 2012, Medical School of Nanchang University, Nanchang, 330006, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Xie
authors.affiliation: Undergraduate student of grade 2012, Medical School of Nanchang University, Nanchang, 330006, People's Republic of China.
authors.forename: Shiyao
authors.initials: S
authors.lastname: Wen
authors.affiliation: Undergraduate student of grade 2012, Medical School of Nanchang University, Nanchang, 330006, People's Republic of China.
authors.forename: Qiuyu
authors.initials: Q
authors.lastname: Xie
authors.affiliation: Department of Physiology, Medical School of Nanchang University, Nanchang, 330006, People's Republic of China.
authors.forename: Shuangmei
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Physiology, Medical School of Nanchang University, Nanchang, 330006, People's Republic of China.
authors.forename: Guilin
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Physiology, Medical School of Nanchang University, Nanchang, 330006, People's Republic of China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Department of Physiology, Medical School of Nanchang University, Nanchang, 330006, People's Republic of China.
authors.forename: Hong
authors.initials: H
authors.lastname: Xu
authors.affiliation: Department of Physiology, Medical School of Nanchang University, Nanchang, 330006, People's Republic of China.
authors.forename: Shouyu
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Physiology, Medical School of Nanchang University, Nanchang, 330006, People's Republic of China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Xue
authors.affiliation: Department of Physiology, Medical School of Nanchang University, Nanchang, 330006, People's Republic of China.
authors.forename: Bing
authors.initials: B
authors.lastname: Wu
authors.affiliation: Department of Physiology, Medical School of Nanchang University, Nanchang, 330006, People's Republic of China.
authors.forename: Qiulan
authors.initials: Q
authors.lastname: Lv
authors.affiliation: Department of Physiology, Medical School of Nanchang University, Nanchang, 330006, People's Republic of China.
authors.forename: Mofeng
authors.initials: M
authors.lastname: Ying
authors.affiliation: Department of Physiology, Medical School of Nanchang University, Nanchang, 330006, People's Republic of China.
authors.forename: Xi
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Physiology, Medical School of Nanchang University, Nanchang, 330006, People's Republic of China. liangsd@hotmail.com.
authors.forename: Shangdong
authors.initials: S
authors.lastname: Liang
mesh_terms: D000818:Animals; D016207:Cytokines / Q000096:biosynthesis; D007150:Immunohistochemistry; D017202:Myocardial Ischemia / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism*; D009206:Myocardium / Q000201:enzymology; D058920:Purinergic P2X Receptor Antagonists / Q000494:pharmacology; D062085:RNA, Long Noncoding / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D034741:RNA, Small Interfering; D051381:Rats; D017207:Rats, Sprague-Dawley; D058486:Receptors, Purinergic P2X7 / Q000235:genetics* / Q000378:metabolism*; D013233:Stellate Ganglion / Q000378:metabolism*
chemical_list: D016207:Cytokines; D058920:Purinergic P2X Receptor Antagonists; D062085:RNA, Long Noncoding; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D058486:Receptors, Purinergic P2X7
keywords: Adenosine triphosphate; Long noncoding RNA; Myocardial ischemia; P2X7 receptor; Stellate ganglia