title: The major proteins of the seed of the fruit of the date palm (Phoenix dactylifera L.): Characterisation and emulsifying properties.
abstract: Proteins were extracted from the seeds of the fruit of the date palm. Proteomic analysis and SDS-PAGE electrophoresis of the extracted proteome suggested it is composed predominantly of the storage proteins glycinin and β-conglycinin, although over 300 proteins were detected, 91 of which were identified with confidence. In terms of protein type, the largest numbers of proteins were associated, not unexpectedly, with metabolism and energy functions, which reflected the requirements of the germinating and growing embryonic plant. The emulsifying properties of the extracted proteins were determined. Date seed protein exhibited a lower emulsifying activity than either whey protein concentrate or soy protein isolate, at each of the pH values tested. However, the stability of the emulsions produced with all three proteins was very similar at the different pH values. This combination of large emulsion droplet size and high emulsion stability properties suggested that the date proteins may adsorb as large protein oligomers.
authors.affiliation: Department of Food & Beverage Science, School of Life Sciences, Heriot-Watt University, Edinburgh EH14 4AS, United Kingdom.
authors.forename: Ibrahim
authors.initials: I
authors.lastname: Akasha
authors.affiliation: Department of Food & Beverage Science, School of Life Sciences, Heriot-Watt University, Edinburgh EH14 4AS, United Kingdom.
authors.forename: Lydia
authors.initials: L
authors.lastname: Campbell
authors.affiliation: Department of Food & Beverage Science, School of Life Sciences, Heriot-Watt University, Edinburgh EH14 4AS, United Kingdom.
authors.forename: Julien
authors.initials: J
authors.lastname: Lonchamp
authors.affiliation: Department of Food & Beverage Science, School of Life Sciences, Heriot-Watt University, Edinburgh EH14 4AS, United Kingdom. Electronic address: S.R.Euston@hw.ac.uk.
authors.forename: Stephen R
authors.initials: SR
authors.lastname: Euston
mesh_terms: D004591:Electrophoresis, Polyacrylamide Gel; D004655:Emulsions / Q000737:chemistry; D005638:Fruit / Q000737:chemistry* / Q000378:metabolism; D065749:Phoeniceae / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry* / Q000378:metabolism; D020543:Proteome / Q000737:chemistry / Q000378:metabolism; D040901:Proteomics; D012639:Seeds / Q000737:chemistry* / Q000378:metabolism
chemical_list: D004655:Emulsions; D010940:Plant Proteins; D020543:Proteome
keywords: Date seed proteins; Emulsifying properties; Proteomics