title: Forensic genetic value of a 27 Y-STR loci multiplex (Yfiler(®) Plus kit) in an Italian population sample.
abstract: The analysis of Y chromosome short tandem repeat (Y-STR) haplotypes provides important information that can be used for investigative purposes and in population studies. The Yfiler(®) Plus PCR Amplification kit (Yfiler(®) Plus, Thermo Fisher Scientific, Waltham, MA, USA) allows the multiplex amplification of 27 Y-STRs, including 7 rapidly mutating markers (RM Y-STRs). In this study, 203 unrelated males from Italy, which were subdivided into 4 different geographical groups (North, Center, South and Sardinia) were analyzed. Several intra-population diversity indexes were computed and compared to those obtained using only loci either from the minimal haplotype or the 17-plex (Yfiler(®), Thermo Fisher Scientific, Waltham, MA, USA). In addition, inter-population diversity analysis (RST) among the four Italian samples was performed. The same analysis was also used to compare the Italian sub-sets to other European populations where the Yfiler(®) Plus haplotype frequency data were available. The Sardinians were significantly differentiated from the other three Italian groups, thus requiring a specific sub-national Y-STR haplotype database. The Yfiler(®) Plus kit showed a high power of discrimination which is useful for criminal investigations, principally due to the inclusion of RM Y-STRs.
authors.affiliation: Carabinieri, Reparto Investigazioni Scientifiche di Roma, Sezione di Biologia, Rome, Italy.
authors.forename: Cesare
authors.initials: C
authors.lastname: Rapone
authors.affiliation: Dipartimento di Biologia e Biotecnologie "Charles Darwin", Sapienza Università di Roma, Rome, Italy.
authors.forename: Eugenia
authors.initials: E
authors.lastname: D'Atanasio
authors.affiliation: Thermo Fisher Scientific, Monza, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Agostino
authors.affiliation: Carabinieri, Reparto Investigazioni Scientifiche di Roma, Sezione di Biologia, Rome, Italy.
authors.forename: Martina
authors.initials: M
authors.lastname: Mariano
authors.affiliation: Dipartimento di Chimica e Tecnologie del Farmaco, Sapienza Università di Roma, Rome, Italy.
authors.forename: Maria Teresa
authors.initials: MT
authors.lastname: Papaluca
authors.affiliation: Dipartimento di Biologia e Biotecnologie "Charles Darwin", Sapienza Università di Roma, Rome, Italy. Electronic address: fulvio.cruciani@uniroma1.it.
authors.forename: Fulvio
authors.initials: F
authors.lastname: Cruciani
authors.affiliation: Carabinieri, Reparto Investigazioni Scientifiche di Roma, Sezione di Biologia, Rome, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Berti
mesh_terms: D041322:Chromosomes, Human, Y; D004247:DNA / Q000032:analysis; D016172:DNA Fingerprinting / Q000379:methods*; D005260:Female; D053803:Forensic Genetics / Q000379:methods*; D005787:Gene Frequency; D005828:Genetics, Population; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D007558:Italy; D008297:Male; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction
chemical_list: D004247:DNA
keywords: Italian population; Rapidly mutating Y-markers; Sardinian population; Y-STR; Yfiler(®) plus