title: Wound Healing Effects of Rose Placenta in a Mouse Model of Full-Thickness Wounds.
abstract: BACKGROUND
  Rosa damascena, a type of herb, has been used for wound healing in Eastern folk medicine. The goal of this study was to evaluate the effectiveness of rose placenta from R. damascena in a full-thickness wound model in mice.
  
  
  METHODS
  Sixty six-week-old C57BL/6N mice were used. Full-thickness wounds were made with an 8-mm diameter punch. Two wounds were made on each side of the back, and wounds were assigned randomly to the control and experimental groups. Rose placenta (250 µg) was injected in the experimental group, and normal saline was injected in the control group. Wound sizes were measured with digital photography, and specimens were harvested. Immunohistochemical staining was performed to assess the expression of epidermal growth factor (EGF), vascular endothelial growth factor (VEGF), transforming growth factor-β1 (TGF-β1), and CD31. Vessel density was measured. Quantitative analysis using an enzyme-linked immunosorbent assay (ELISA) for EGF was performed. All evaluations were performed on postoperative days 0, 2, 4, 7, and 10. Statistical analyses were performed using the paired t-test.
  
  
  RESULTS
  On days 4, 7, and 10, the wounds treated with rose placenta were significantly smaller. On day 2, VEGF and EGF expression increased in the experimental group. On days 7 and 10, TGF-β1 expression decreased in the experimental group. On day 10, vessel density increased in the experimental group. The increase in EGF on day 2 was confirmed with ELISA.
  
  
  CONCLUSIONS
  Rose placenta was found to be associated with improved wound healing in a mouse full-thickness wound model via increased EGF release. Rose placenta may potentially be a novel drug candidate for enhancing wound healing.
authors.affiliation: Department of Plastic and Reconstructive Surgery, Gil Medical Center, Gachon University School of Medicine, Incheon, Korea.
authors.forename: Yang Woo
authors.initials: YW
authors.lastname: Kim
authors.affiliation: Department of Plastic and Reconstructive Surgery, Gil Medical Center, Gachon University School of Medicine, Incheon, Korea.
authors.forename: Seung Ryeol
authors.initials: SR
authors.lastname: Baek
authors.affiliation: Department of Plastic and Reconstructive Surgery, Gil Medical Center, Gachon University School of Medicine, Incheon, Korea.
authors.forename: Eun Sook
authors.initials: ES
authors.lastname: Lee
authors.affiliation: Department of Pathology, Gil Medical Center, Gachon University School of Medicine, Incheon, Korea.
authors.forename: Sang Ho
authors.initials: SH
authors.lastname: Lee
authors.affiliation: Anti-Aging Research Institute, Bio-FD&C Co., LTD, Incheon, Korea.
authors.forename: Sang Hyun
authors.initials: SH
authors.lastname: Moh
authors.affiliation: Anti-Aging Research Institute, Bio-FD&C Co., LTD, Incheon, Korea.
authors.forename: Soo Yun
authors.initials: SY
authors.lastname: Kim
authors.affiliation: Anti-Aging Research Institute, Bio-FD&C Co., LTD, Incheon, Korea.
authors.forename: Ji Hong
authors.initials: JH
authors.lastname: Moh
authors.affiliation: Ginza Tomato, Tokyo, Japan.
authors.forename: Chieko
authors.initials: C
authors.lastname: Kondo
authors.affiliation: Department of Plastic and Reconstructive Surgery, Gil Medical Center, Gachon University School of Medicine, Incheon, Korea.
authors.forename: Young Woo
authors.initials: YW
authors.lastname: Cheon
keywords: Epidermal growth factor; Mice; Rosa; Wound healing