title: High potential of adhesion to biotic and abiotic surfaces by opportunistic Staphylococcus aureus strains isolated from orthodontic appliances.
abstract: Orthodontic and other oral appliances act as reservoir of opportunistic pathogens that can easily become resistant to antibiotics and cause systemic infections. The aim of this study was to investigate the ability of Staphylococcus aureus strains isolated from healthy patients with orthodontic appliances, to adhere to biotic (HeLa cells) and abiotic surfaces (polystyrene and dental alloy). Adhesive ability to polystyrene was tested by crystal violet staining and quantitative biofilm production on dental alloy surfaces was evaluated by MTT reduction assay. In addition, the presence of icaA and icaD genes was achieved by polymerase chain reaction (PCR). Qualitative biofilm production revealed that 70.6% of strains were slime producers. The metabolic activity of S. aureus biofilms on dental alloy surfaces was high and did not differ between tested strains. Moreover, all the isolates were adhesive to HeLa cells and 94% of them harbor icaA and icaD genes. Considerable adhesion and internalization capacity to the epithelial HeLa cells and strong biofilm production abilities together, with a high genotypic expression of icaA/icaD genes are an important equipment of S. aureus to colonize orthodontic appliances and eventually to disseminate towards other body areas.
authors.affiliation: Laboratory of Infectious Diseases and Biological Agents (LR99ES27), Faculty of Pharmacy, Monastir University, Monastir, Tunisia. Electronic address: Abderrahmen_merghni@yahoo.fr.
authors.forename: Abderrahmen
authors.initials: A
authors.lastname: Merghni
authors.affiliation: Laboratory of Infectious Diseases and Biological Agents (LR99ES27), Faculty of Pharmacy, Monastir University, Monastir, Tunisia.
authors.forename: Mouna
authors.initials: M
authors.lastname: Ben Nejma
authors.affiliation: Dento-Facial Orthopedics Department of Monastir Dental Clinic, Laboratory of Oral Health and Orofacial Rehabilitation (LR12ES11), Tunisia.
authors.forename: Ines
authors.initials: I
authors.lastname: Dallel
authors.affiliation: Dento-Facial Orthopedics Department of Monastir Dental Clinic, Laboratory of Oral Health and Orofacial Rehabilitation (LR12ES11), Tunisia.
authors.forename: Samir
authors.initials: S
authors.lastname: Tobji
authors.affiliation: Dento-Facial Orthopedics Department of Monastir Dental Clinic, Laboratory of Oral Health and Orofacial Rehabilitation (LR12ES11), Tunisia.
authors.forename: Adel
authors.initials: A
authors.lastname: Ben Amor
authors.affiliation: BioImaging Center Lille-FR3642, Lille, France.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Janel
authors.affiliation: BioImaging Center Lille-FR3642, Lille, France; Cellular Microbiology and Physics of Infection Group, Center of Infection and Immunity of Lille: CNRS UMR8204, INSERM U1019, Institut Pasteur de Lille, Lille University, France.
authors.forename: Frank
authors.initials: F
authors.lastname: Lafont
authors.affiliation: Laboratory of Infectious Diseases and Biological Agents (LR99ES27), Faculty of Pharmacy, Monastir University, Monastir, Tunisia.
authors.forename: Mahjoub
authors.initials: M
authors.lastname: Aouni
authors.affiliation: Laboratory of Infectious Diseases and Biological Agents (LR99ES27), Faculty of Pharmacy, Monastir University, Monastir, Tunisia; Laboratory of Microbiology, University Hospital of Fattouma Bourguiba, Monastir, Tunisia.
authors.forename: Maha
authors.initials: M
authors.lastname: Mastouri
mesh_terms: D000293:Adolescent; D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D018441:Biofilms; D004866:Equipment Contamination / Q000706:statistics & numerical data*; D005260:Female; D006801:Humans; D008297:Male; D009967:Orthodontic Appliances / Q000382:microbiology*; D013203:Staphylococcal Infections / Q000382:microbiology; D013211:Staphylococcus aureus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D055815:Young Adult
chemical_list: D001426:Bacterial Proteins
keywords: Adhesion; Biofilm; HeLa cells; Orthodontic appliances; Staphylococcus aureus