title: Acinetobacter equisp. nov., isolated from horse faeces.
abstract: The taxonomic position of five strains isolated from horse faeces, and which shared identical 16S rRNA gene sequences, were studied. Cells of all isolates are Gram-stain-negative, obligately aerobic and have a rod-shaped appearance. The strains show highest 16S rRNA gene sequence similarities toAcinetobacter lwoffii(98.3 %),Acinetobacter haemolyticus(98.0 %),Acienetobacter johnsonii(97.9 %) andAcinetobacter brisouii(97.9 %). Whole-genome sequencing of strain 114Tand phylogeny reconstruction based on a core set of 1061Acinetobactergenes indicated thatA. bouvetiiCIP 107468Twas the closest relative among species of the genusAcinetobacterfor which whole genome sequences are available. The genomic DNA G+C content of strain 114Tis 34.9 mol%, which is lower than any other value reported for the genusAcinetobacter. The predominant polyamine is 1,3-diaminopropane, which is typical for the genusAcinetobacter. The most abundant fatty acids are C16 : 1ω7cand/or iso-C15 : 02-OH (36 %) and C16 : 0(28 %). The proportion of C18 : 1ω9c(7 %) is distinctively low compared to most species of the genus. The major ubiquinone of strain 114Tis Q-9. Microscopic studies revealed the presence of pili and the absence of flagella. The capability of all five strains to utilize l-arabinose and gentisate as well as their lack of growth at temperatures of 41 °C and above provide sufficient criteria to distinguish the isolates from all species of the genusAcinetobacterwith validly published names. Based on these combined data, the five isolates represent a novel species of the genusAcinetobacter, for which the nameAcinetobacter equisp. nov. is proposed. The type strain is 114T( = DSM 27228T= CCUG 65204T).
authors.affiliation: Robert Koch Institute, Wernigerode Branch, Burgstr. 37, D-38855 Wernigerode, Germany.
authors.forename: Marie T
authors.initials: MT
authors.lastname: Poppel
authors.affiliation: Robert Koch Institute, Wernigerode Branch, Burgstr. 37, D-38855 Wernigerode, Germany.
authors.forename: Evelyn
authors.initials: E
authors.lastname: Skiebe
authors.affiliation: Robert Koch Institute, Advanced Light and Electron Microscopy (ZBS 4), Seestr. 11, D-13353 Berlin, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Laue
authors.affiliation: Institute for Cell Biology and Neuroscience, Goethe University Frankfurt, Max-von-Laue-Str. 13, D-60438 Frankfurt am Main, Germany.
authors.forename: Holger
authors.initials: H
authors.lastname: Bergmann
authors.affiliation: Institute for Cell Biology and Neuroscience, Goethe University Frankfurt, Max-von-Laue-Str. 13, D-60438 Frankfurt am Main, Germany.
authors.forename: Ingo
authors.initials: I
authors.lastname: Ebersberger
authors.affiliation: Robert Koch Institute, Wernigerode Branch, Burgstr. 37, D-38855 Wernigerode, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Garn
authors.affiliation: Robert Koch Institute, Wernigerode Branch, Burgstr. 37, D-38855 Wernigerode, Germany.
authors.forename: Angelika
authors.initials: A
authors.lastname: Fruth
authors.affiliation: Division of Clinical Microbiology and Infection Biology, Institute of Bacteriology, Mycology and Hygiene, University of Veterinary Medicine, A-1210 Vienna, Austria.
authors.forename: Sandra
authors.initials: S
authors.lastname: Baumgardt
authors.affiliation: Division of Clinical Microbiology and Infection Biology, Institute of Bacteriology, Mycology and Hygiene, University of Veterinary Medicine, A-1210 Vienna, Austria.
authors.forename: Hans-Jürgen
authors.initials: HJ
authors.lastname: Busse
authors.affiliation: Robert Koch Institute, Wernigerode Branch, Burgstr. 37, D-38855 Wernigerode, Germany.
authors.forename: Gottfried
authors.initials: G
authors.lastname: Wilharm