title: A second-generation expression system for tyrosine-sulfated proteins and its application in crop protection.
abstract: Posttranslational modification (PTM) of proteins and peptides is important for diverse biological processes in plants and animals. The paucity of heterologous expression systems for PTMs and the technical challenges associated with chemical synthesis of these modified proteins has limited detailed molecular characterization and therapeutic applications. Here we describe an optimized system for expression of tyrosine-sulfated proteins in Escherichia coli and its application in a bio-based crop protection strategy in rice.
authors.affiliation: Department of Plant Pathology and the Genome Center, University of California, Davis, Davis, CA 95616, USA. pcronald@ucdavis.edu.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Schwessinger
authors.forename: Xiang
authors.initials: X
authors.lastname: Li
authors.forename: Thomas L
authors.initials: TL
authors.lastname: Ellinghaus
authors.forename: Leanne Jade G
authors.initials: LJ
authors.lastname: Chan
authors.forename: Tong
authors.initials: T
authors.lastname: Wei
authors.forename: Anna
authors.initials: A
authors.lastname: Joe
authors.forename: Nicholas
authors.initials: N
authors.lastname: Thomas
authors.forename: Rory
authors.initials: R
authors.lastname: Pruitt
authors.forename: Paul D
authors.initials: PD
authors.lastname: Adams
authors.forename: Maw Sheng
authors.initials: MS
authors.lastname: Chern
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Petzold
authors.forename: Chang C
authors.initials: CC
authors.lastname: Liu
authors.forename: Pamela C
authors.initials: PC
authors.lastname: Ronald
mesh_terms: D000383:Agriculture / Q000379:methods; D000069601:Crop Protection / Q000379:methods; D018556:Crops, Agricultural; D004926:Escherichia coli / Q000235:genetics*; D049452:Green Fluorescent Proteins / Q000235:genetics; D012275:Oryza / Q000235:genetics / Q000382:microbiology*; D010455:Peptides / Q000737:chemistry; D010957:Plasmids / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000737:chemistry; D058615:Synthetic Biology / Q000379:methods*; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000737:chemistry
chemical_list: D010455:Peptides; D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins; C005662:tyrosine O-sulfate; D014443:Tyrosine