title: Study on Prevalence, Antibiotic Susceptibility, and tuf Gene Sequence-Based Genotyping of Species-Level of Coagulase-Negative Staphylococcus Isolated From Keratitis Caused by Using Soft Contact Lenses.
abstract: OBJECTIVE
  To study on antibiotic susceptibility and identify coagulase-negative Staphylococcus (CoNS) species based on tuf gene sequencing from keratitis followed by using soft contact lenses in Isfahan, Iran, 2013.
  
  
  METHODS
  This study examined 77 keratitis cases. The samples were cultured and the isolation of CoNS was done by phenotypic tests, and in vitro sensitivity testing was done by Kirby-Bauer disk diffusion susceptibility method.
  
  
  RESULTS
  Thirty-eight of isolates were conveniently identified as CoNS. In this study, 27 (71.1%), 21 (55.3%), and 16 (42.1%) were resistant to penicillin, erythromycin, and tetracycline, respectively. One hundred percent of isolates were sensitive to gentamicin, and 36 (94.7%) and 33 (86.8%) of isolates were sensitive to chloramphenicol and ciprofloxacin, respectively. Also, resistances to cefoxitin were 7 (18.4%). Analysis of tuf gene proved to be discriminative and sensitive in which all the isolates were identified with 99.0% similarity to reference strains, and Staphylococcus epidermidis had the highest prevalence among other species.
  
  
  CONCLUSIONS
  Results of this study showed that CoNS are the most common agents causing contact lens-associated microbial keratitis, and the tuf gene sequencing analysis is a reliable method for distinguishing CoNS species. Also gentamycin, chloramphenicol, and ciprofloxacin are more effective than the other antibacterial agents against these types of bacteria.
authors.affiliation: Departments of Microbiology and Virology (J.F., H.F., H.G.S., B.N.E., N.S.H., S.M.), and Ophthalmology (A.Z., A.P.), School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran; and Department of Microbiology and Virology (M.S., S.B., G.O.), School of Medicine and Student Research Committee, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Jamshid
authors.initials: J
authors.lastname: Faghri
authors.forename: Alireza
authors.initials: A
authors.lastname: Zandi
authors.forename: Alireza
authors.initials: A
authors.lastname: Peiman
authors.forename: Hossein
authors.initials: H
authors.lastname: Fazeli
authors.forename: Bahram Nasr
authors.initials: BN
authors.lastname: Esfahani
authors.forename: Hajieh Ghasemian
authors.initials: HG
authors.lastname: Safaei
authors.forename: Nafiseh Sadat
authors.initials: NS
authors.lastname: Hosseini
authors.forename: Sina
authors.initials: S
authors.lastname: Mobasherizadeh
authors.forename: Mansour
authors.initials: M
authors.lastname: Sedighi
authors.forename: Samaneh
authors.initials: S
authors.lastname: Burbur
authors.forename: Golfam
authors.initials: G
authors.lastname: Oryan
mesh_terms: D000328:Adult; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D003263:Contact Lenses, Hydrophilic / Q000009:adverse effects*; D004269:DNA, Bacterial / Q000235:genetics; D004352:Drug Resistance, Microbial; D005260:Female; D005838:Genotype; D006801:Humans; D007634:Keratitis / Q000453:epidemiology / Q000209:etiology / Q000382:microbiology*; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D010444:Peptide Elongation Factor Tu / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D015995:Prevalence; D013203:Staphylococcal Infections / Q000453:epidemiology / Q000209:etiology / Q000382:microbiology*; D013210:Staphylococcus / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification; D055815:Young Adult
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D010444:Peptide Elongation Factor Tu