title: MicroRNA-18a regulates invasive meningiomas via hypoxia-inducible factor-1α.
abstract: The aim of the present study was to investigate the effects of microRNA-18a (miR-18a) on the invasiveness and metastasis of invasive meningiomas and the underlying mechanism. A total of 69 patients with meningiomas (30 patients in the invasive meningioma group and 39 patients in the non-invasive meningioma group) and 48 cases in the control group were enrolled. Samples of meningioma tissues, serum and cerebrospinal fluid were collected. Reverse transcription-quantitative polymerase chain reaction was performed to quantify the expression levels of hypoxia-inducible factor-1α (HIF-1α) mRNA and miR-18a. Western blot analysis was used to determine protein expression levels of HIF-1α. The expression levels of HIF-1α mRNA and protein in all three types of sample from the invasive meningioma group were significantly higher compared with those in the control and non-invasive meningioma groups (P<0.05), and the expression levels of HIF-1α mRNA in the serum and cerebrospinal fluid of the non-invasive meningioma group were significantly higher compared with those in the control group (P<0.05). The expression levels of miR-18a in the invasive meningioma group were significantly reduced compared with those in the control and non-invasive meningioma groups (P<0.05), whereas the levels of miR-18a in the non-invasive meningioma group were significantly lower compared with those in the control group (P<0.05). The expression of HIF-1α is significantly upregulated in patients with invasive meningiomas, possibly due to the downregulation of miR-18a expression. Therefore, miR-18a may regulate invasive meningiomas via HIF-1α.
authors.affiliation: Department of Neurosurgery, Laiwu City People's Hospital, Laiwu, Shandong 271100, P.R. China.
authors.forename: Puxian
authors.initials: P
authors.lastname: Li
authors.affiliation: Department of Neurosurgery, Laiwu City People's Hospital, Laiwu, Shandong 271100, P.R. China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Department of Neurosurgery, Laiwu City People's Hospital, Laiwu, Shandong 271100, P.R. China.
authors.forename: Fengjia
authors.initials: F
authors.lastname: Li
authors.affiliation: Department of Neurosurgery, Laiwu City People's Hospital, Laiwu, Shandong 271100, P.R. China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Pan
authors.affiliation: Department of Neurosurgery, Laiwu City People's Hospital, Laiwu, Shandong 271100, P.R. China.
authors.forename: Zhenrui
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Neurosurgery, Laiwu City People's Hospital, Laiwu, Shandong 271100, P.R. China.
authors.forename: Xiangdong
authors.initials: X
authors.lastname: Lu
authors.affiliation: Department of Neurosurgery, Laiwu City People's Hospital, Laiwu, Shandong 271100, P.R. China.
authors.forename: Chunyu
authors.initials: C
authors.lastname: Song
authors.affiliation: Department of Neurosurgery, Laiwu City People's Hospital, Laiwu, Shandong 271100, P.R. China.
authors.forename: Xingtao
authors.initials: X
authors.lastname: Diao
keywords: hypoxia-inducible factor-1α; invasiveness; meningioma; microRNA-18a