title: Aggregation of Ribosomal Protein S6 at Nucleolus Is Cell Cycle-Controlled and Its Function in Pre-rRNA Processing Is Phosphorylation Dependent.
abstract: Ribosomal protein S6 (rpS6) has long been regarded as one of the primary r-proteins that functions in the early stage of 40S subunit assembly, but its actual role is still obscure. The correct forming of 18S rRNA is a key step in the nuclear synthesis of 40S subunit. In this study, we demonstrate that rpS6 participates in the processing of 30S pre-rRNA to 18S rRNA only when its C-terminal five serines are phosphorylated, however, the process of entering the nucleus and then targeting the nucleolus does not dependent its phosphorylation. Remarkably, we also find that the aggregation of rpS6 at the nucleolus correlates to the phasing of cell cycle, beginning to concentrate in the nucleolus at later S phase and disaggregate at M phase. J. Cell. Biochem. 117: 1649-1657, 2016. © 2015 Wiley Periodicals, Inc.
authors.affiliation: Department of Clinical Genetics and Experimental Medicine, Fuzhou General Hospital, Xiamen University School of Medicine, Fuzhou, Fujian, 350025, P. R. China.|Beijing Institute of Biotechnology, Beijing, 100071, P. R. China.
authors.forename: Duo
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Beijing Institute of Biotechnology, Beijing, 100071, P. R. China.
authors.forename: Hui-Peng
authors.initials: HP
authors.lastname: Chen
authors.affiliation: Beijing Institute of Radiation Medicine, Beijing, 100850, P. R. China.
authors.forename: Hai-Feng
authors.initials: HF
authors.lastname: Duan
authors.affiliation: Beijing Institute of Biotechnology, Beijing, 100071, P. R. China.
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Gao
authors.affiliation: Beijing Institute of Biotechnology, Beijing, 100071, P. R. China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Shao
authors.affiliation: Beijing Institute of Biotechnology, Beijing, 100071, P. R. China.
authors.forename: Ke-Yan
authors.initials: KY
authors.lastname: Chen
authors.affiliation: Beijing Institute of Biotechnology, Beijing, 100071, P. R. China.
authors.forename: You-Liang
authors.initials: YL
authors.lastname: Wang
authors.affiliation: Department of Clinical Genetics and Experimental Medicine, Fuzhou General Hospital, Xiamen University School of Medicine, Fuzhou, Fujian, 350025, P. R. China.
authors.forename: Feng-Hua
authors.initials: FH
authors.lastname: Lan
authors.affiliation: Beijing Institute of Biotechnology, Beijing, 100071, P. R. China.
authors.forename: Xian-Wen
authors.initials: XW
authors.lastname: Hu
mesh_terms: D002455:Cell Division / Q000502:physiology; D002466:Cell Nucleolus / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D010766:Phosphorylation / Q000502:physiology; D066329:Protein Aggregates / Q000502:physiology*; D012322:RNA Precursors / Q000378:metabolism*; D012323:RNA Processing, Post-Transcriptional / Q000502:physiology*; D012337:RNA, Ribosomal, 18S / Q000378:metabolism*; D038601:Ribosomal Protein S6 / Q000378:metabolism*; D016196:S Phase / Q000502:physiology
chemical_list: D066329:Protein Aggregates; D012322:RNA Precursors; D012337:RNA, Ribosomal, 18S; D038601:Ribosomal Protein S6
keywords: 40S RIBOSOMAL SUBUNIT; CELL CYCLE; PHOSPHORYLATION; PRE-rRNA; RIBOSOMAL PROTEIN S6