title: Impact of OmpR on the membrane proteome of Yersinia enterocolitica in different environments: repression of major adhesin YadA and heme receptor HemR.
abstract: Enteropathogenic Yersinia enterocolitica is able to grow within or outside the mammalian host. Previous transcriptomic studies have indicated that the regulator OmpR plays a role in the expression of hundreds of genes in enterobacteria. Here, we have examined the impact of OmpR on the production of Y. enterocolitica membrane proteins upon changes in temperature, osmolarity and pH. Proteomic analysis indicated that the loss of OmpR affects the production of 120 proteins, a third of which are involved in uptake/transport, including several that participate in iron or heme acquisition. A set of proteins associated with virulence was also affected. The influence of OmpR on the abundance of adhesin YadA and heme receptor HemR was examined in more detail. OmpR was found to repress YadA production and bind to the yadA promoter, suggesting a direct regulatory effect. In contrast, the repression of hemR expression by OmpR appears to be indirect. These findings provide new insights into the role of OmpR in remodelling the cell surface and the adaptation of Y. enterocolitica to different environmental niches, including the host.
authors.affiliation: Department of Applied Microbiology, Institute of Microbiology, Faculty of Biology, University of Warsaw, Ilji Miecznikowa 1, Warsaw, 02-096, Poland.
authors.forename: Marta
authors.initials: M
authors.lastname: Nieckarz
authors.affiliation: Department of Applied Microbiology, Institute of Microbiology, Faculty of Biology, University of Warsaw, Ilji Miecznikowa 1, Warsaw, 02-096, Poland.
authors.forename: Adrianna
authors.initials: A
authors.lastname: Raczkowska
authors.affiliation: Mass Spectrometry Laboratory, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawińskiego 5a, Warsaw, 02-106, Poland.
authors.forename: Janusz
authors.initials: J
authors.lastname: Dębski
authors.affiliation: Mass Spectrometry Laboratory, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawińskiego 5a, Warsaw, 02-106, Poland.
authors.forename: Michał
authors.initials: M
authors.lastname: Kistowski
authors.affiliation: Institute of Genetics and Biotechnology, Faculty of Biology, University of Warsaw, Pawińskiego 5a, Warsaw, 02-106, Poland.|Mass Spectrometry Laboratory, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawińskiego 5a, Warsaw, 02-106, Poland.
authors.forename: Michał
authors.initials: M
authors.lastname: Dadlez
authors.affiliation: Max von Pettenkofer Institute for Hygiene and Medical Microbiology, Ludwig Maximilians University, Pettenkoferstrasse 9a, Munich, 80336, Germany.
authors.forename: Jürgen
authors.initials: J
authors.lastname: Heesemann
authors.affiliation: Max von Pettenkofer Institute for Hygiene and Medical Microbiology, Ludwig Maximilians University, Pettenkoferstrasse 9a, Munich, 80336, Germany.
authors.forename: Ombeline
authors.initials: O
authors.lastname: Rossier
authors.affiliation: Department of Applied Microbiology, Institute of Microbiology, Faculty of Biology, University of Warsaw, Ilji Miecznikowa 1, Warsaw, 02-096, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Brzostek
mesh_terms: D018829:Adhesins, Bacterial / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D009994:Osmolar Concentration; D011401:Promoter Regions, Genetic; D020543:Proteome / Q000378:metabolism; D040901:Proteomics; D011956:Receptors, Cell Surface / Q000096:biosynthesis* / Q000235:genetics; D015534:Trans-Activators / Q000235:genetics*; D014774:Virulence; D015008:Yersinia enterocolitica / Q000235:genetics*
chemical_list: D018829:Adhesins, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; C078168:HemR protein, Yersinia enterocolitica; D020543:Proteome; D011956:Receptors, Cell Surface; D015534:Trans-Activators; C064832:YadA protein, Yersinia; C112177:osmolarity response regulator proteins