title: Antibacterial activity of Baccharis dracunculifolia in planktonic cultures and biofilms of Streptococcus mutans.
abstract: Streptococcus mutans is an important cariogenic microorganism, and alternative methods for its elimination are required. Different concentrations of Baccharis dracunculifolia essential oil (EO) were tested to determine its minimal inhibitory concentration (MIC) in planktonic cultures, and this concentration was used in S. mutans biofilms. Additionally, we assessed the effect of a 0.12% chlorhexidine (CHX) and saline solution in S. mutans biofilms. The biofilms were grown in discs of composite resin for 48h and exposed to B. dracunculifolia, CHX or saline solution for 5min. The viability of the biofilms was determined by counting the colony-forming units per milliliter (CFU/ml) in agar, which was statistically significant (P<0.05). The MIC of the B. dracunculifolia EO to planktonic growth of S. mutans was 6%. In biofilms of S. mutans clinical isolates, B. dracunculifolia EO (6%) and CHX resulted in reductions of 53.3-91.1% and 79.1-96.6%, respectively. For the biofilm formed by the S. mutans reference strain, the reductions achieved with B. dracunculifolia EO and CHX were, respectively, 39.3% and 88.1%. It was concluded that B. dracunculifolia EO showed antibacterial activity and was able to control this oral microorganism, which otherwise causes dental caries.
authors.affiliation: Unesp - Univ Estadual Paulista, School of Dentistry, Institute of Science and Technology, Department of Biosciences and Oral Diagnosis, Francisco José Longo 777, São Dimas, São José dos Campos, CEP: 12245-000 SP, Brazil. Electronic address: cricabio@gmail.com.
authors.forename: Cristiane A
authors.initials: CA
authors.lastname: Pereira
authors.affiliation: Unesp - Univ Estadual Paulista, School of Dentistry, Institute of Science and Technology, Department of Biosciences and Oral Diagnosis, Francisco José Longo 777, São Dimas, São José dos Campos, CEP: 12245-000 SP, Brazil. Electronic address: carol_biolog@yahoo.com.br.
authors.forename: Anna Carolina B Pereira
authors.initials: AC
authors.lastname: Costa
authors.affiliation: Unitau - Univ de Taubaté, School of Dentistry, Department of Restorative Dentistry, Expedicionário Ernesto Pereira 110, Centro, Taubaté, CEP: 12020-330 SP, Brazil. Electronic address: prili@yahoo.com.br.
authors.forename: Priscila Christiane S
authors.initials: PC
authors.lastname: Liporoni
authors.affiliation: Unitau - Univ de Taubaté, School of Dentistry, Department of Restorative Dentistry, Expedicionário Ernesto Pereira 110, Centro, Taubaté, CEP: 12020-330 SP, Brazil. Electronic address: marcosrego@uol.com.br.
authors.forename: Marcos A
authors.initials: MA
authors.lastname: Rego
authors.affiliation: Unesp - Univ Estadual Paulista, School of Dentistry, Institute of Science and Technology, Department of Biosciences and Oral Diagnosis, Francisco José Longo 777, São Dimas, São José dos Campos, CEP: 12245-000 SP, Brazil. Electronic address: olavojorge@fosjc.unesp.br.
authors.forename: Antonio Olavo C
authors.initials: AO
authors.lastname: Jorge
mesh_terms: D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000494:pharmacology*; D031175:Baccharis / Q000737:chemistry*; D018441:Biofilms / Q000187:drug effects*; D002710:Chlorhexidine / Q000494:pharmacology; D015169:Colony Count, Microbial; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability / Q000187:drug effects; D009822:Oils, Volatile / Q000302:isolation & purification / Q000494:pharmacology*; D012965:Sodium Chloride / Q000494:pharmacology; D013295:Streptococcus mutans / Q000187:drug effects* / Q000502:physiology
chemical_list: D000900:Anti-Bacterial Agents; D009822:Oils, Volatile; D012965:Sodium Chloride; D002710:Chlorhexidine
keywords: Baccharis; Biofilms; Dental caries; Streptococcus mutans