title: Successive Administration of Streptococcus Type 5 Group A Antigens and S. typhimurium Antigenic Complex Corrects Elevation of Serum Cytokine Concentration and Number of Bone Marrow Stromal Pluripotent Cells in CBA Mice Induced by Each Antigen Separately.
abstract: Administration of bacterial antigens to CBA mice induced an increase in serum concentration of virtually all cytokines with a peak in 4 h after administration of S. typhimurium antigens and in 7 h after administration of streptococcus antigens. In 20 h, cytokine concentrations returned to the control level or were slightly below it. In 4 h after administration of S. typhimurium antigens preceded 3 h before by administration of streptococcus antigens, we observed a significant decrease in serum concentrations of IFN-γ, IL-10, GM-CSF, IL-12, and TNF-α, in comparison with injection S. typhimurium antigens alone and IL-5, IL-10, GM-CSF, and TNF-α in comparison with injection of streptococcus antigens alone; the concentrations of IL-2 and IFN-γ, in contrast, increased by 1.5 times in this case. In 20 h after administration of S. typhimurium antigens, the number of multipotential stromal cells (MSC) in the bone marrow and their cloning efficiency (ECF-MSC) increased by 4.8 and 4.4 times, respectively, in comparison with the control, while after administration of streptococcus antigens by 2.6 and 2.4 times, respectively. In 20 h after administration of S. typhimurium antigens preceded 3 h before by administration of streptococcus antigens, these parameters increased by 3.2 and 2.9 times, respectively, in comparison with the control, i.e. the observed increase in the level of MSC count and ECF-MSC is more consistent with the response of the stromal tissue to streptococcus antigens. Thus, successive administration of two bacterial antigens corrected both serum cytokine profiles and MSC response to administration of each antigen separately, which indicates changeability of the stromal tissue in response to changes in the immune response.
authors.affiliation: Laboratory of Immunity Regulation and Immune Tolerance, Moscow, Russia. uliya.gorskaya@nearmedic.ru.|Laboratory of Bioactive Nanostructures, Moscow, Russia. uliya.gorskaya@nearmedic.ru.
authors.forename: Yu F
authors.initials: YF
authors.lastname: Gorskaya
authors.affiliation: Laboratory of Microbiology of Latent Infections; N. F. Gamaleya Research Institute of Epidemiology and Microbiology, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: T A
authors.initials: TA
authors.lastname: Danilova
authors.affiliation: Laboratory of Immunity Regulation and Immune Tolerance, Moscow, Russia.|Laboratory of Bioactive Nanostructures, Moscow, Russia.
authors.forename: V I
authors.initials: VI
authors.lastname: Grabko
authors.affiliation: Laboratory of Immunity Regulation and Immune Tolerance, Moscow, Russia.|Laboratory of Bioactive Nanostructures, Moscow, Russia.
authors.forename: V G
authors.initials: VG
authors.lastname: Nesterenko
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology*; D016207:Cytokines / Q000097:blood*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000378:metabolism; D016753:Interleukin-10 / Q000378:metabolism; D018664:Interleukin-12 / Q000378:metabolism; D008297:Male; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D051379:Mice; D008808:Mice, Inbred CBA; D039904:Pluripotent Stem Cells / Q000166:cytology*; D012486:Salmonella typhimurium / Q000276:immunology*; D013297:Streptococcus pyogenes / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D000942:Antigens, Bacterial; D016207:Cytokines; C508609:IL10 protein, human; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D018664:Interleukin-12; D016178:Granulocyte-Macrophage Colony-Stimulating Factor
keywords: cytokine profile of blood serum; immune response; mesenchymal stem cells; successive administration of antigens