title: Transgenic n-3 PUFAs enrichment leads to weight loss via modulating neuropeptides in hypothalamus.
abstract: Body weight is related to fat mass, which is associated with obesity. Our study explored the effect of fat-1 gene on body weight in fat-1 transgenic mice. In present study, we observed that the weight/length ratio of fat-1 transgenic mice was lower than that of wild-type mice. The serum levels of triglycerides (TG), cholesterol (CT), high-density lipoprotein cholesterol (HDL-c), low-density lipoprotein cholesterol (LDL-c) and blood glucose (BG) in fat-1 transgenic mice were all decreased. The weights of peri-bowels fat, perirenal fat and peri-testicular fat in fat-1 transgenic mice were reduced. We hypothesized that increase of n-3 PUFAs might alter the expression of hypothalamic neuropeptide genes and lead to loss of body weight in fat-1 transgenic mice. Therefore, we measured mRNA levels of appetite neuropeptides, Neuropeptide Y (NPY), Agouti-related peptides (AgRP), Proopiomelanocortin (POMC), Cocaine and amphetamine regulated transcript (CART), ghrelin and nesfatin-1 in hypothalamus by real-time PCR. Compared with wild-type mice, the mRNA levels of CART, POMC and ghrelin were higher, while the mRNA levels of NPY, AgRP and nesfatin-1 were lower in fat-1 transgenic mice. The results indicate that fat-1 gene or n-3 PUFAs participates in regulation of body weight, and the mechanism of this phenomenon involves the expression of appetite neuropeptides and lipoproteins in fat-1 transgenic mice.
authors.affiliation: Department of Biochemistry and Molecular Biology, Medical College of Qingdao University, Qingdao, Shandong 266021, PR China.
authors.forename: Shuangshuang
authors.initials: S
authors.lastname: Ma
authors.affiliation: Department of Biochemistry and Molecular Biology, Medical College of Qingdao University, Qingdao, Shandong 266021, PR China.
authors.forename: Yinlin
authors.initials: Y
authors.lastname: Ge
authors.affiliation: Department of Biochemistry and Molecular Biology, Medical College of Qingdao University, Qingdao, Shandong 266021, PR China.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Gai
authors.affiliation: Department of Biochemistry and Molecular Biology, Medical College of Qingdao University, Qingdao, Shandong 266021, PR China.
authors.forename: Meilan
authors.initials: M
authors.lastname: Xue
authors.affiliation: Department of Biochemistry and Molecular Biology, Medical College of Qingdao University, Qingdao, Shandong 266021, PR China.
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.affiliation: Mathazhusazhu General Hospital, Harvard Medical College, Boston, USA.
authors.forename: Jingxuan
authors.initials: J
authors.lastname: Kang
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, China.
authors.forename: Jianbo
authors.initials: J
authors.lastname: Wan
authors.affiliation: Department of Biochemistry and Molecular Biology, Medical College of Qingdao University, Qingdao, Shandong 266021, PR China. Electronic address: zhangjinyu6768@163.com.
authors.forename: Jinyu
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000273:Adipose Tissue / Q000033:anatomy & histology; D000818:Animals; D001066:Appetite / Q000235:genetics; D001786:Blood Glucose / Q000378:metabolism; D049628:Body Size / Q000235:genetics; D015820:Cadherins / Q000235:genetics*; D002784:Cholesterol / Q000097:blood; D015525:Fatty Acids, Omega-3 / Q000378:metabolism*; D007031:Hypothalamus / Q000378:metabolism*; D008297:Male; D008822:Mice, Transgenic; D009479:Neuropeptides / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D014280:Triglycerides / Q000097:blood; D015431:Weight Loss / Q000235:genetics*
chemical_list: D001786:Blood Glucose; D015820:Cadherins; D015525:Fatty Acids, Omega-3; D009479:Neuropeptides; D012333:RNA, Messenger; D014280:Triglycerides; C502406:fat1 protein, mouse; D002784:Cholesterol
keywords: Body weight; Fat-1 gene; Hypothalamus; Neuropeptide