title: Molecular Characterization and TRAP Analysis of Gene in Dendranthema morifolium.
abstract: The molecular maker system - TRAP was applied to develop a novel and more accurate method to identify the variety and establish the evolutionary relationship of different categories of Dendranthema morifolium. A software - GENESIS 2.4 was used to conduct the cluster analysis and genetic dendrogram establishment. The results showed that 202 different fragments were amplified with 6 pair primers using the TRAP marker system. The polymorphic fragments number is 45, which takes up to 22.3%. The cluster analysis showed that 4 materials used in this study can be classified into 2 main groups and 3 subgroups. The genetic identity is 0.0767 and the average genetic distance is 0.9236 among the four materials. A new tool using the TRAP marker system is more accurate and can be used to identify different categories of Dendranthema morifolium at molecular level.
authors.affiliation: Guangxi Medical University, Nanning Pharmaceutical college Guangxi China.
authors.forename: Y
authors.initials: Y
authors.lastname: Luo
authors.affiliation: Guangxi Medical University, Nanning Pharmaceutical college Guangxi China.
authors.forename: Q
authors.initials: Q
authors.lastname: Li
authors.affiliation: Guangxi Medical University, Nanning Pharmaceutical college Guangxi China.
authors.forename: X
authors.initials: X
authors.lastname: Wang
authors.affiliation: Guangxi Medical University, Nanning Pharmaceutical college Guangxi China.
authors.forename: F
authors.initials: F
authors.lastname: Yang
authors.affiliation: Guangxi Medical University, Nanning Pharmaceutical college Guangxi China.
authors.forename: S
authors.initials: S
authors.lastname: Nong
authors.affiliation: Guangxi Medical University, Nanning Pharmaceutical college Guangxi China.
authors.forename: Q
authors.initials: Q
authors.lastname: Li
authors.affiliation: Guangxi Medical University, Nanning Pharmaceutical college Guangxi China zhudan_zhudan@sina.cn.
authors.forename: D
authors.initials: D
authors.lastname: Zhu
mesh_terms: D005075:Biological Evolution; D002681:China; D031202:Chrysanthemum / Q000235:genetics*; D016000:Cluster Analysis; D004365:Drugs, Chinese Herbal; D020224:Expressed Sequence Tags; D005819:Genetic Markers; D005821:Genetic Techniques; D005828:Genetics, Population; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D012984:Software
chemical_list: D004365:Drugs, Chinese Herbal; D005819:Genetic Markers