title: Functional characterization of the tumor suppressor CMTM8 and its association with prognosis in bladder cancer.
abstract: Previous research revealed that CMTM8 acts as a tumor suppressor gene in variety cancers. However, the role of CMTM8 in bladder cancer has never been reported. In this study, the expression profile of CMTM8 was examined in bladder cancer tissues and bladder cancer cell lines. The effects of CMTM8 on bladder cancer cell proliferation, apoptosis, migration, and invasion were examined. Bladder tumor tissues from 84 cases were examined for CMTM8 expression by immunohistochemistry. Disease-specific survival was investigated using a Kaplan-Meier analysis, and Cox proportional hazards analysis was assessed. Our results showed that upregulation of CMTM8 in the T24 cell line could suppress T24 cells proliferation, migration and invasion and enhance the sensitivity to Epirubicin. Kaplan-Meier analysis revealed that the expression of CMTM8 was correlated with the survival time of bladder cancer patients. Altogether, our data suggested that CMTM8 is an important tumor suppressor gene in human bladder cancer and qualified as a useful prognostic indicator for patients with bladder cancer.
authors.affiliation: Department of Urology, Peking University People's Hospital, 11 Xizhimen Street, Xicheng District, Beijing, 100044, China.
authors.forename: Shiying
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Immunology, School of Basic Medical Sciences, and Key Laboratory of Medical Immunology of Ministry of Health, Peking University Health Science Center, 38 Xueyuan Road, Beijing, 100191, China.
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Pei
authors.affiliation: Department of Urology, Peking University People's Hospital, 11 Xizhimen Street, Xicheng District, Beijing, 100044, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Hu
authors.affiliation: National Institutes for Food and Drug Control, Beijing, 100053, China.
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Immunology, School of Basic Medical Sciences, and Key Laboratory of Medical Immunology of Ministry of Health, Peking University Health Science Center, 38 Xueyuan Road, Beijing, 100191, China.
authors.forename: Xiaoning
authors.initials: X
authors.lastname: Mo
authors.affiliation: Department of Immunology, School of Basic Medical Sciences, and Key Laboratory of Medical Immunology of Ministry of Health, Peking University Health Science Center, 38 Xueyuan Road, Beijing, 100191, China.
authors.forename: Quansheng
authors.initials: Q
authors.lastname: Song
authors.affiliation: Department of Immunology, School of Basic Medical Sciences, and Key Laboratory of Medical Immunology of Ministry of Health, Peking University Health Science Center, 38 Xueyuan Road, Beijing, 100191, China.
authors.forename: Yingmei
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Urology, Peking University People's Hospital, 11 Xizhimen Street, Xicheng District, Beijing, 100044, China. cavinx@sina.com.
authors.forename: Kexin
authors.initials: K
authors.lastname: Xu
authors.affiliation: Department of Immunology, School of Basic Medical Sciences, and Key Laboratory of Medical Immunology of Ministry of Health, Peking University Health Science Center, 38 Xueyuan Road, Beijing, 100191, China. yw@bjmu.edu.cn.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Urology, Peking University People's Hospital, 11 Xizhimen Street, Xicheng District, Beijing, 100044, China.
authors.forename: Yanqun
authors.initials: Y
authors.lastname: Na
mesh_terms: D000328:Adult; D000368:Aged; D017209:Apoptosis / Q000235:genetics; D014408:Biomarkers, Tumor / Q000096:biosynthesis / Q000235:genetics*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D018925:Chemokines / Q000096:biosynthesis / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053208:Kaplan-Meier Estimate; D062792:MARVEL Domain-Containing Proteins / Q000096:biosynthesis / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D025521:Tumor Suppressor Proteins / Q000235:genetics; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; C507076:CMTM8 protein, human; D018925:Chemokines; D062792:MARVEL Domain-Containing Proteins; D025521:Tumor Suppressor Proteins
keywords: Bladder cancer; CMTM8; Prognosis; Survival; Tumor suppressor