title: MiR542-3p Regulates the Epithelial-Mesenchymal Transition by Directly Targeting BMP7 in NRK52e.
abstract: Accumulating evidence demonstrated that miRNAs are highly involved in kidney fibrosis and Epithelial-Eesenchymal Transition (EMT), however, the mechanisms of miRNAs in kidney fibrosis are poorly understood. In this work, we identified that miR542-3p could promote EMT through down-regulating bone morphogenetic protein 7 (BMP7) expression by targeting BMP7 3'UTR. Firstly, real-time PCR results showed that miR542-3p was significantly up-regulated in kidney fibrosis in vitro and in vivo. Moreover, Western blot results demonstrated that miR542-3p may promote EMT in the NRK52e cell line. In addition, we confirmed that BMP7, which played a crucial role in anti-kidney fibrosis and suppressed the progression of EMT, was a target of miR542-3p through Dual-Luciferase reporter assay, as did Western blot analysis. The effects of miR542-3p on regulating EMT could also be suppressed by transiently overexpressing BMP7 in NRK52e cells. Taken together, miR542-3p may be a critical mediator of the induction of EMT via directly targeting BMP7.
authors.affiliation: The Division of Molecular Nephrology and the Creative Training Center for Undergraduates, The M.O.E. Key Laboratory of Laboratory Medical Diagnostics, the College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. liuzhicheng323@163.com.
authors.forename: Zhicheng
authors.initials: Z
authors.lastname: Liu
authors.affiliation: The Seventh Class of 2012 year entry, the Third Clinical College, Chongqing Medical University, Chongqing 400016, China. zhouyuru93@163.com.
authors.forename: Yuru
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: The Division of Molecular Nephrology and the Creative Training Center for Undergraduates, The M.O.E. Key Laboratory of Laboratory Medical Diagnostics, the College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. yyokyy1126@hotmail.com.
authors.forename: Yue
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: The Division of Molecular Nephrology and the Creative Training Center for Undergraduates, The M.O.E. Key Laboratory of Laboratory Medical Diagnostics, the College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. nifang168@163.com.
authors.forename: Fang
authors.initials: F
authors.lastname: Nie
authors.affiliation: The Division of Molecular Nephrology and the Creative Training Center for Undergraduates, The M.O.E. Key Laboratory of Laboratory Medical Diagnostics, the College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. pengrui911@foxmail.com.
authors.forename: Rui
authors.initials: R
authors.lastname: Peng
authors.affiliation: The Division of Molecular Nephrology and the Creative Training Center for Undergraduates, The M.O.E. Key Laboratory of Laboratory Medical Diagnostics, the College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. lqianyincqmu@163.com.
authors.forename: Qianyin
authors.initials: Q
authors.lastname: Li
authors.affiliation: The Division of Molecular Nephrology and the Creative Training Center for Undergraduates, The M.O.E. Key Laboratory of Laboratory Medical Diagnostics, the College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. Zhongshilyu@163.com.
authors.forename: Zhongshi
authors.initials: Z
authors.lastname: Lyu
authors.affiliation: The Division of Molecular Nephrology and the Creative Training Center for Undergraduates, The M.O.E. Key Laboratory of Laboratory Medical Diagnostics, the College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. mao1204086118@163.com.
authors.forename: Zhaomin
authors.initials: Z
authors.lastname: Mao
authors.affiliation: The Division of Molecular Nephrology and the Creative Training Center for Undergraduates, The M.O.E. Key Laboratory of Laboratory Medical Diagnostics, the College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. lyhuang0603@sina.com.
authors.forename: Liyuan
authors.initials: L
authors.lastname: Huang
authors.affiliation: The Division of Molecular Nephrology and the Creative Training Center for Undergraduates, The M.O.E. Key Laboratory of Laboratory Medical Diagnostics, the College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. shmily900519@foxmail.com.
authors.forename: Li
authors.initials: L
authors.lastname: Zhou
authors.affiliation: The Division of Molecular Nephrology and the Creative Training Center for Undergraduates, The M.O.E. Key Laboratory of Laboratory Medical Diagnostics, the College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. 18715854036@sina.cn.
authors.forename: Yiman
authors.initials: Y
authors.lastname: Li
authors.affiliation: The Division of Molecular Nephrology and the Creative Training Center for Undergraduates, The M.O.E. Key Laboratory of Laboratory Medical Diagnostics, the College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. lanyxiu@163.com.
authors.forename: Jing
authors.initials: J
authors.lastname: Hao
authors.affiliation: The Division of Molecular Nephrology and the Creative Training Center for Undergraduates, The M.O.E. Key Laboratory of Laboratory Medical Diagnostics, the College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. cqmunds@163.com.
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Ni
authors.affiliation: The Division of Molecular Nephrology and the Creative Training Center for Undergraduates, The M.O.E. Key Laboratory of Laboratory Medical Diagnostics, the College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. qiannijincqmu@sina.com.
authors.forename: Qianni
authors.initials: Q
authors.lastname: Jin
authors.affiliation: The Division of Molecular Nephrology and the Creative Training Center for Undergraduates, The M.O.E. Key Laboratory of Laboratory Medical Diagnostics, the College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. lys960110@sina.com.
authors.forename: Yaoshui
authors.initials: Y
authors.lastname: Long
authors.affiliation: The Division of Molecular Nephrology and the Creative Training Center for Undergraduates, The M.O.E. Key Laboratory of Laboratory Medical Diagnostics, the College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. 18883936591@163.com.
authors.forename: Pan
authors.initials: P
authors.lastname: Ju
authors.affiliation: The Division of Molecular Nephrology and the Creative Training Center for Undergraduates, The M.O.E. Key Laboratory of Laboratory Medical Diagnostics, the College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. yuwen0717@sina.com.
authors.forename: Wen
authors.initials: W
authors.lastname: Yu
authors.affiliation: The Division of Molecular Nephrology and the Creative Training Center for Undergraduates, The M.O.E. Key Laboratory of Laboratory Medical Diagnostics, the College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. keithljn@sina.cn.
authors.forename: Jianing
authors.initials: J
authors.lastname: Liu
authors.affiliation: The Division of Molecular Nephrology and the Creative Training Center for Undergraduates, The M.O.E. Key Laboratory of Laboratory Medical Diagnostics, the College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. hyx_zuichu@outlook.com.
authors.forename: Yanxia
authors.initials: Y
authors.lastname: Hu
authors.affiliation: The Division of Molecular Nephrology and the Creative Training Center for Undergraduates, The M.O.E. Key Laboratory of Laboratory Medical Diagnostics, the College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. zhouqin@cqmu.edu.cn.
authors.forename: Qin
authors.initials: Q
authors.lastname: Zhou
mesh_terms: D000818:Animals; D001665:Binding Sites; D055419:Bone Morphogenetic Protein 7 / Q000737:chemistry / Q000235:genetics*; D002460:Cell Line; D004195:Disease Models, Animal; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics*; D005355:Fibrosis; D005786:Gene Expression Regulation; D006801:Humans; D007674:Kidney Diseases / Q000235:genetics / Q000473:pathology; D051379:Mice; D035683:MicroRNAs / Q000737:chemistry / Q000235:genetics*; D034622:RNA Interference; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics; D053773:Transforming Growth Factor beta1 / Q000378:metabolism
chemical_list: D055419:Bone Morphogenetic Protein 7; C572357:MIRN542 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; D053773:Transforming Growth Factor beta1
keywords: Epithelial-Eesenchymal Transition (EMT); bone morphogenetic protein 7 (BMP7); kidney fibrosis; miR542-3p; transforming growth factor beta 1 (TGFβ1)