title: Next generation sequencing applications for breast cancer research.
abstract: For some time, cancer has not been thought of as a disease, but as a multifaceted, heterogeneous complex of genotypic and phenotypic manifestations leading to tumorigenesis. Due to recent technological progress, the outcome of cancer patients can be greatly improved by introducing in clinical practice the advantages brought about by the development of next generation sequencing techniques. Biomedical suppliers have come up with various applications which medical researchers can use to characterize a patient's disease from molecular and genetic point of view in order to provide caregivers with rapid and relevant information to guide them in choosing the most appropriate course of treatment, with maximum efficiency and minimal side effects. Breast cancer, whose incidence has risen dramatically, is a good candidate for these novel diagnosis and therapeutic approaches, particularly when referring to specific sequencing panels which are designed to detect germline or somatic mutations in genes that are involved in breast cancer tumorigenesis and progression. Benchtop next generation sequencing machines are becoming a more common presence in the clinical setting, empowering physicians to better treat their patients, by offering early diagnosis alternatives, targeted remedies, and bringing medicine a step closer to achieving its ultimate goal, personalized therapy.
authors.affiliation: Functional Genomics, Proteomics and Experimental Pathology Department, Prof. Dr. I. Chiricuta Oncology Institute, Cluj-Napoca, Romania ; Research Center for Functional Genomics, Biomedicine and Translational Medicine, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, Romania ; Faculty of Biology and Geology, Babes Bolyai Univesity, Cluj-Napoca, Romania.
authors.forename: Roxana Cojocneanu
authors.initials: RC
authors.lastname: Petric
authors.affiliation: Functional Genomics, Proteomics and Experimental Pathology Department, Prof. Dr. I. Chiricuta Oncology Institute, Cluj-Napoca, Romania ; Research Center for Functional Genomics, Biomedicine and Translational Medicine, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, Romania.
authors.forename: Laura-Ancuta
authors.initials: LA
authors.lastname: Pop
authors.affiliation: Functional Genomics, Proteomics and Experimental Pathology Department, Prof. Dr. I. Chiricuta Oncology Institute, Cluj-Napoca, Romania.
authors.forename: Ancuta
authors.initials: A
authors.lastname: Jurj
authors.affiliation: Functional Genomics, Proteomics and Experimental Pathology Department, Prof. Dr. I. Chiricuta Oncology Institute, Cluj-Napoca, Romania ; University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Romania.
authors.forename: Lajos
authors.initials: L
authors.lastname: Raduly
authors.affiliation: 2nd Department of Internal Medicine, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, Romania.
authors.forename: Dan
authors.initials: D
authors.lastname: Dumitrascu
authors.affiliation: Taxonomy and Ecology Department, NIRDBS - Institute of Biological Research, Cluj-Napoca, Romania.
authors.forename: Nicolae
authors.initials: N
authors.lastname: Dragos
authors.affiliation: Functional Genomics, Proteomics and Experimental Pathology Department, Prof. Dr. I. Chiricuta Oncology Institute, Cluj-Napoca, Romania ; Research Center for Functional Genomics, Biomedicine and Translational Medicine, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, Romania ; Department of Experimental Therapeutics, MD Anderson Cancer Center, Houston, Texas, USA ; Department of Immunology, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, Romania.
authors.forename: Ioana Berindan
authors.initials: IB
authors.lastname: Neagoe
keywords: benchtop sequencers; breast cancer; genome; next generation sequencing (NGS); personalized therapy