title: The protective effect of tadalafil on IMA (ischemia modified albumin) levels in experimental renal ischemia-reperfusion injury.
abstract: BACKGROUND
  To investigate the effect of the tadalafil in experimental renal I/R injury and to evaluate these changes with IMA (nonspesific early biomarker of ischemia), NO and MDA levels.
  
  
  METHODS
  Twenty four female Wistar rats were randomly divided into 3 groups (n=8): Group I, sham; Group II, 60 min I/R; Group III, 60 min I/R plus tadalafil. Tadalafil was administered via an orogastric tube (10 mg/kg) 24 h prior to the procedure. After ischemia of the left kidney and 1 h of reperfusion, blood samples were obtained, and the kidney was removed.
  
  
  RESULTS
  Statistically significant histopathologic changes were exist between groups, with the most severe injury was determined in group II in comparison to the others (X(2)=21,803, P=0.000). Also mean serum IMA levels were higher in group II, but not statistically significant (19.83±7.81 U/ml, 22.26±7.14 U/ml and 19.82±7.77 U/ml, P=0.613). In addition, NO values were lower in I/R groups (P=0.049). There were no differences among the groups in terms of MDA.
  
  
  CONCLUSIONS
  IMA may be used as a nonselective biomarker for IR injury before the occurrence of necrosis. Decreased IMA levels may indicate the nephroprotective effect of tadalafil in renal IR injury.
authors.affiliation: Department of Urology, School of Medicine, Adnan Menderes University Aydin, Turkey.
authors.forename: Akin Soner
authors.initials: AS
authors.lastname: Amasyali
authors.affiliation: Department of Urology, School of Medicine, Adnan Menderes University Aydin, Turkey.
authors.forename: Abdullah
authors.initials: A
authors.lastname: Akkurt
authors.affiliation: Department of Urology, School of Medicine, Adnan Menderes University Aydin, Turkey.
authors.forename: Ercan
authors.initials: E
authors.lastname: Kazan
authors.affiliation: Department of Biochemistry, School of Medicine, Adnan Menderes University Aydin, Turkey.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Yilmaz
authors.affiliation: Department of Urology, Faculty of Medicine, Istanbul Medeniyet University Istanbul, Turkey.
authors.forename: Bulent
authors.initials: B
authors.lastname: Erol
authors.affiliation: Department of Physiology School of Medicine, Adnan Menderes University Aydin, Turkey.
authors.forename: Yuksel
authors.initials: Y
authors.lastname: Yildiz
authors.affiliation: Department of Urology, School of Medicine, Adnan Menderes University Aydin, Turkey.
authors.forename: Haluk
authors.initials: H
authors.lastname: Erol
keywords: Tadalafil; ischemia modified albumin; renal ischemia reperfusion injury