title: Combined urea-thin layer chromatography and silver nitrate-thin layer chromatography for micro separation and determination of hard-to-detect branched chain fatty acids in natural lipids.
abstract: A simple, fast and efficient procedure was developed for micro separation and enrichment of branched chain fatty acids (BCFA) from natural products using successive thin layer chromatography (TLC) technique coupling novel urea-TLC with AgNO3-TLC, which rely on the formation of urea adduction and AgNO3 bonding in methanol. These natural lipids contain a significant amount of straight chain fatty acids (FA). Fresh and fast urea-TLC and AgNO3-TLC plate making techniques were developed with more even coating and less coating material contamination before being utilized for separation. Goat milk fat was used as a model. Various experimental parameters that affect urea-TLC and AgNO3-TLC separation of BCFA were investigated and optimized, including coating of urea, concentration of original oil sample, mobile phase and sample application format. High efficiency of removal of straight chain FA was achieved with a low amount of sample in an easy and fast way. A total BCFA mix with much higher purity than previous studies was successfully achieved. The developed method has also been applied for the concentration and analysis of BCFA in cow milk fat and Anchovy oil.
authors.affiliation: State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, No. 1800 Lihu Ave., Wuxi 214122, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Yan
authors.affiliation: State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, No. 1800 Lihu Ave., Wuxi 214122, China. Electronic address: wangxg1002@gmail.com.
authors.forename: Xingguo
authors.initials: X
authors.lastname: Wang
authors.affiliation: Food Inspection Authority of Zhangjiagang Entry-Exit Inspection and Quarantine Bureau, Zhangjiagang 215600, China.
authors.forename: Yijun
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Wuxi Maternity and Child Health Care Hospital, Wuxi 212422, China.
authors.forename: Jingying
authors.initials: J
authors.lastname: Xiang
authors.affiliation: State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, No. 1800 Lihu Ave., Wuxi 214122, China.
authors.forename: Xiaosan
authors.initials: X
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, No. 1800 Lihu Ave., Wuxi 214122, China.
authors.forename: Huijun
authors.initials: H
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, No. 1800 Lihu Ave., Wuxi 214122, China.
authors.forename: Yunping
authors.initials: Y
authors.lastname: Yao
authors.affiliation: State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, No. 1800 Lihu Ave., Wuxi 214122, China.
authors.forename: Ruijie
authors.initials: R
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, No. 1800 Lihu Ave., Wuxi 214122, China.
authors.forename: Xiaoqiang
authors.initials: X
authors.lastname: Zou
authors.affiliation: State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, No. 1800 Lihu Ave., Wuxi 214122, China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, No. 1800 Lihu Ave., Wuxi 214122, China.
authors.forename: Qingzhe
authors.initials: Q
authors.lastname: Jin
mesh_terms: D000818:Animals; D002417:Cattle; D002855:Chromatography, Thin Layer / Q000379:methods; D005227:Fatty Acids / Q000032:analysis*; D005260:Female; D005395:Fish Oils / Q000737:chemistry; D006041:Goats; D007202:Indicators and Reagents; D008055:Lipids / Q000737:chemistry*; D008892:Milk / Q000737:chemistry; D012835:Silver Nitrate; D014508:Urea
chemical_list: D005227:Fatty Acids; D005395:Fish Oils; D007202:Indicators and Reagents; D008055:Lipids; D014508:Urea; D012835:Silver Nitrate
keywords: AgNO(3); BCFA; GC–MS; Thin layer chromatography; Urea