title: Beneficial Influence of Short-Term Germination on Decreasing Allergenicity of Peanut Proteins.
abstract: Most allergenic storage proteins in peanuts are degraded during seed germination. By altering this natural physiological process, it might be possible to reduce peanut protein allergenicity. However, little is known about the change in allergenic proteins and their corresponding immunocreactivity, and the effects of major environmental conditions on their allergenicity during germination. In this study, the influence of different germination conditions (temperature and light) on the degradation of Ara h1 and allergenicity changes of peanut seeds was evaluated by ELISA and Western blotting. The results showed that the 40- and 65-kDa proteins in peanut seeds degraded rapidly during the time course, beginning at 60 (at 25 °C) and 108 h (at 20 °C), and the corresponding immunocreactivity of Ara h1 decreased approximately one-third after 5 to 7 d of germination. Compared with the cotyledons, the embryonic axes had a higher proportion of Ara h1, which was then degraded relatively faster during germination, resulting in a significant reduction in its allergenicity. Although a higher temperature improved the seed germination rate, it affected sprout quality (as did light); therefore, 25 °C and dark surroundings were suitable conditions under which peanut sprouts were processed; neither factor significantly affected the allergenicity of Ara h1. These results provided a theoretical basis for studies using biological methods to reduce peanut allergenicity.
authors.affiliation: School of Food Science and Technology, Jiangnan Univ, Wu, Xi, 214122, China.
authors.forename: Yingchao
authors.initials: Y
authors.lastname: Li
authors.affiliation: School of Food Science and Technology, Jiangnan Univ, Wu, Xi, 214122, China.
authors.forename: Xiulan
authors.initials: X
authors.lastname: Sun
authors.affiliation: School of Food Science and Technology, Jiangnan Univ, Wu, Xi, 214122, China.
authors.forename: Zhezhe
authors.initials: Z
authors.lastname: Ma
authors.affiliation: Inst. of Agricultural Products Processing, Ningbo Inst. of Agricultural Sciences, Ningbo, 315040, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Cui
authors.affiliation: School of Food Science and Technology, Jiangnan Univ, Wu, Xi, 214122, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Du
authors.affiliation: School of Food Science and Technology, Jiangnan Univ, Wu, Xi, 214122, China.
authors.forename: Xiuhua
authors.initials: X
authors.lastname: Xia
authors.affiliation: School of Food Science and Technology, Jiangnan Univ, Wu, Xi, 214122, China.
authors.forename: He
authors.initials: H
authors.lastname: Qian
mesh_terms: D000485:Allergens / Q000276:immunology*; D000818:Animals; D000906:Antibodies / Q000097:blood; D052179:Antigens, Plant / Q000276:immunology / Q000378:metabolism*; D010367:Arachis / Q000378:metabolism*; D015153:Blotting, Western; D005511:Food Handling / Q000379:methods*; D005512:Food Hypersensitivity / Q000097:blood / Q000517:prevention & control; D018525:Germination; D006023:Glycoproteins / Q000276:immunology / Q000378:metabolism*; D006358:Hot Temperature; D008027:Light; D008297:Male; D008565:Membrane Proteins; D009754:Nuts / Q000378:metabolism*; D010940:Plant Proteins / Q000276:immunology / Q000378:metabolism*; D011817:Rabbits; D036226:Seedlings; D012639:Seeds / Q000378:metabolism
chemical_list: D000485:Allergens; D000906:Antibodies; D052179:Antigens, Plant; C500889:Ara h 1 protein, Arachis hypogaea; D006023:Glycoproteins; D008565:Membrane Proteins; D010940:Plant Proteins
keywords: Ara h1; allergenic protein, allergenicity; germination; peanut seed