title: Metabolic analysis of canine peripheral blood mononuclear cells treated ex vivo with dexamethasone.
abstract: In dogs, hyperadrenocorticism (HAC) is associated with insulin resistance and diabetes does progress with HAC. There are significant differences in the transcriptomic and proteomic patterns of activated T cells, which parallel the findings in muscle tissues. The aim of this study was to assess how glucocorticoids affect intracellular metabolites in canine peripheral blood mononuclear cells (CnPBMCs) using dexamethasone. A total of 96 metabolites were identified by capillary electrophoresis time-of-flight mass spectrometry (CE-TOFMS). After incubation with dexamethasone, the metabolites glucose 1-phosphate, glucose 6-phosphate, fructose 6-phosphate, sedoheptulose 7-phosphate and acetyl-CoA were significantly increased. However, ATP, CTP, dATP, pyruvic acid and NADP(+) were significantly decreased. These results show that a glucocorticoid reduces the catabolic reaction of glucose and accordingly decreases the glucose requirements of CnPBMCs.
authors.affiliation: Department of Veterinary Science, School of Veterinary Medicine, Nippon Veterinary and Life Science University, 1-7-1 Kyonancho, Musashino, Tokyo 180-8602, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Nozawa
authors.affiliation: Department of Veterinary Science, School of Veterinary Medicine, Nippon Veterinary and Life Science University, 1-7-1 Kyonancho, Musashino, Tokyo 180-8602, Japan.
authors.forename: Touko
authors.initials: T
authors.lastname: Sato
authors.affiliation: Department of Veterinary Science, School of Veterinary Medicine, Nippon Veterinary and Life Science University, 1-7-1 Kyonancho, Musashino, Tokyo 180-8602, Japan.
authors.forename: Kinya
authors.initials: K
authors.lastname: Katayama
authors.affiliation: Department of Veterinary Nursing and Technology, School of Veterinary Medicine, Nippon Veterinary and Life Science University, 1-7-1 Kyonancho, Musashino, Tokyo 180-8602, Japan.
authors.forename: Katsumi
authors.initials: K
authors.lastname: Ishioka
authors.affiliation: Department of Veterinary Nursing and Technology, School of Veterinary Medicine, Nippon Veterinary and Life Science University, 1-7-1 Kyonancho, Musashino, Tokyo 180-8602, Japan.
authors.forename: Toshinori
authors.initials: T
authors.lastname: Sako
authors.affiliation: Department of Veterinary Science, School of Veterinary Medicine, Nippon Veterinary and Life Science University, 1-7-1 Kyonancho, Musashino, Tokyo 180-8602, Japan.
authors.forename: Toshiro
authors.initials: T
authors.lastname: Arai
authors.affiliation: Department of Veterinary Science, School of Veterinary Medicine, Nippon Veterinary and Life Science University, 1-7-1 Kyonancho, Musashino, Tokyo 180-8602, Japan. Electronic address: tazaki@nvlu.ac.jp.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Tazaki
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002952:Citric Acid Cycle / Q000187:drug effects; D003907:Dexamethasone / Q000494:pharmacology*; D004285:Dogs; D005938:Glucocorticoids / Q000494:pharmacology*; D006019:Glycolysis; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000378:metabolism
chemical_list: D005938:Glucocorticoids; D003907:Dexamethasone; D000255:Adenosine Triphosphate
keywords: Capillary electrophoresis time-of-flight mass spectrometry; Dexamethasone; Dog; Metabolites; Peripheral blood mononuclear cells