title: Characterization and sub-cellular localization of GalNAc-binding proteins isolated from human hepatic stellate cells.
abstract: Although the expression levels of total GalNAc-binding proteins (GNBPs) were up-regulated significantly in human hepatic stellate cells (HSCs) activated with transforming growth factor-β1(TGF-β1), yet little is known about the precise types, distribution and sub-cellular localization of the GNBPs in HSCs. Here, 264 GNBPs from the activated HSCs and 257 GNBPs from the quiescent HSCs were identified and annotated. A total of 46 GNBPs were estimated to be significantly up-regulated and 40 GNBPs were estimated to be significantly down-regulated in the activated HSCs. For example, the GNBPs (i.e. BTF3, COX17, and ATP5A1) responsible for the regulation of protein binding were up-regulated, and those (i.e. FAM114A1, ENO3, and TKT) responsible for the regulation of protein binding were down-regulated in the activated HSCs. The motifs of the isolated GNBPs showed that Proline residue had the maximum preference in consensus sequences. The western blotting showed the expression levels of COX17, and PRMT1 were significantly up-regulated, while, the expression level of CLIC1(B5) was down-regulated in the activated HSCs and liver cirrhosis tissues. Moreover, the GNBPs were sub-localized in the Golgi apparatus of HSCs. In conclusion, the precision alteration of the GNBPs referred to pathological changes in liver fibrosis/cirrhosis may provide useful information to find new molecular mechanism of HSC activation and discover the biomarkers for diagnosis of liver fibrosis/cirrhosis as well as development of new anti-fibrotic strategies.
authors.affiliation: Laboratory for Functional Glycomics, College of Life Sciences, Northwest University, Xi'an, 710069, PR China.
authors.forename: Yaogang
authors.initials: Y
authors.lastname: Zhong
authors.affiliation: Laboratory for Functional Glycomics, College of Life Sciences, Northwest University, Xi'an, 710069, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Laboratory for Functional Glycomics, College of Life Sciences, Northwest University, Xi'an, 710069, PR China.
authors.forename: Hanjie
authors.initials: H
authors.lastname: Yu
authors.affiliation: Laboratory for Functional Glycomics, College of Life Sciences, Northwest University, Xi'an, 710069, PR China.
authors.forename: Jiaxu
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Cell Engineering Research Centre and Department of Cell Biology, Fourth Military Medical University, Xi'an, 710032, PR China.
authors.forename: Xiu-Xuan
authors.initials: XX
authors.lastname: Sun
authors.affiliation: Laboratory for Functional Glycomics, College of Life Sciences, Northwest University, Xi'an, 710069, PR China.
authors.forename: Wentian
authors.initials: W
authors.lastname: Chen
authors.affiliation: Cell Engineering Research Centre and Department of Cell Biology, Fourth Military Medical University, Xi'an, 710032, PR China. Electronic address: hjbian@fmmu.edu.cn.
authors.forename: Huijie
authors.initials: H
authors.lastname: Bian
authors.affiliation: Laboratory for Functional Glycomics, College of Life Sciences, Northwest University, Xi'an, 710069, PR China. Electronic address: zhengli@nwu.edu.cn.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Li
mesh_terms: D000116:Acetylgalactosamine / Q000378:metabolism*; D002478:Cells, Cultured; D006031:Glycosylation; D055166:Hepatic Stellate Cells / Q000378:metabolism* / Q000648:ultrastructure*; D006801:Humans; D013347:Subcellular Fractions / Q000378:metabolism*; D014018:Tissue Distribution
chemical_list: D000116:Acetylgalactosamine
keywords: Characterization; GalNAc-binding proteins; Human hepatic stellate cells; Liver fibrosis; Motifs; Sub-cellular localization