title: Efficacy of Disinfection of Dental Stone Casts: Virkon versus Sodium Hypochlorite.
abstract: OBJECTIVE
  The purpose of this experimental study was to compare the disinfection efficacy of sodium hypochlorite and peroxygenic acid (Virkon) solutions for dental stone casts contaminated with microbial strains.
  
  
  METHODS
  A total of 960 spherical stone beads with a diameter of 10 mm were prepared and used as carriers of bacterial inoculums. They were individually inoculated by soaking in broth culture media containing each of the four understudy microorganisms. Different concentrations of Virkon and hypochlorite solutions were prepared using distilled water and then were sprayed on the surfaces of dental casts contaminated with Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus subtilis and Candida albicans. The pour plate technique was used to evaluate the antimicrobial efficacy of each solution. Microbicidal effect (ME) was calculated according to the log10 of control colony counts minus the log10 of the remaining colony counts after the antimicrobial procedure. Statistical difference was assessed using the Kruskal Wallis and the Man Whitney U tests with a significance of 95%.
  
  
  RESULTS
  We observed different bactericidal effects of Virkon at various concentrations; 1% Virkon killed S. aureus, P aeruginosa, and Candida albicans, while 3% Virkon solution was required to kill B. subtilis. For S. aureus, P. aeruginosa, and C. albicans, no significant difference was observed between 1% Virkon and 0.525% sodium hypochlorite (P >0.05). For B. subtilis, the efficacy of 3% Virkon and 0.525% sodium hypochlorite was not significantly different (P >0.999).
  
  
  CONCLUSIONS
  According to the obtained results for Virkon and based on its low toxicity and good environmental compatibility, it may be recommended as an antimicrobial disinfectant for dental stone casts as non-critical items.
authors.affiliation: Assistant Professor, Department of Prosthodontics, Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Elnaz
authors.initials: E
authors.lastname: Moslehifard
authors.affiliation: Associate Professor, Faculty of Pharmacy, Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Farzaneh
authors.initials: F
authors.lastname: Lotfipour
authors.affiliation: Assistant Professor, Department of Prosthodontics, Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Mahmood
authors.initials: M
authors.lastname: Robati Anaraki
authors.affiliation: Assistant Professor, Department of Prosthodontics, Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Elnaz
authors.initials: E
authors.lastname: Shafee
authors.affiliation: Assistant Professor, Department of Pediatric, Faculty of Dentistry, Zanjan University of Medical Sciences, Zanjan, Iran.
authors.forename: Shabnam
authors.initials: S
authors.lastname: Tamjid-Shabestari
authors.affiliation: Assistant Professor, Department of Prosthodontics, Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Tahereh
authors.initials: T
authors.lastname: Ghaffari
keywords: Dental gypsum; Disinfectants; Infection control; Monoperoxysulfate; Sodium hypochlorite