title: Biochemical conversion of sugarcane straw hemicellulosic hydrolyzate supplemented with co-substrates for xylitol production.
abstract: Biotechnological production of xylitol is an attractive route to add value to a sugarcane biorefinery, through utilization of the hemicellulosic fraction of sugarcane straw, whose availability is increasing in Brazil. Herein, supplementation of the sugarcane straw hemicellulosic hydrolyzate (xylose 57gL(-1)) with maltose, sucrose, cellobiose or glycerol was proposed, and their effect as co-substrates on xylitol production by Candida guilliermondii FTI 20037 was studied. Sucrose (10gL(-1)) and glycerol (0.7gL(-1)) supplementation led to significant increase of 8.88% and 6.86% on xylose uptake rate (1.11gL(-1)h(-1) and 1.09gL(-1)), respectively, but only with sucrose, significant increments of 12.88% and 8.69% on final xylitol concentration (36.11gL(-1)) and volumetric productivity (0.75gL(-1)h(-1)), respectively, were achieved. Based on these results, utilization of complex sources of sucrose, derived from agro-industries, as nutritional supplementation for xylitol production can be proposed as a strategy for improving the yeast performance and reducing the cost of this bioprocess by replacing more expensive nutrients.
authors.affiliation: Departamento de Biotecnologia, Escola de Engenharia de Lorena, Universidade de São Paulo, 12602-810 Lorena, São Paulo, Brazil. Electronic address: ahernandez@usp.br.
authors.forename: A F
authors.initials: AF
authors.lastname: Hernández-Pérez
authors.affiliation: Departamento de Biotecnologia, Escola de Engenharia de Lorena, Universidade de São Paulo, 12602-810 Lorena, São Paulo, Brazil.
authors.forename: I A L
authors.initials: IA
authors.lastname: Costa
authors.affiliation: Departamento de Biotecnologia, Escola de Engenharia de Lorena, Universidade de São Paulo, 12602-810 Lorena, São Paulo, Brazil.
authors.forename: D D V
authors.initials: DD
authors.lastname: Silva
authors.affiliation: Departamento de Biotecnologia, Escola de Engenharia de Lorena, Universidade de São Paulo, 12602-810 Lorena, São Paulo, Brazil.
authors.forename: K J
authors.initials: KJ
authors.lastname: Dussán
authors.affiliation: Departamento de Biotecnologia, Escola de Engenharia de Lorena, Universidade de São Paulo, 12602-810 Lorena, São Paulo, Brazil.
authors.forename: T R
authors.initials: TR
authors.lastname: Villela
authors.affiliation: Departamento de Engenharia, Universidade Estadual Paulista "Júlio de Mesquita Filho", 12516-410 Guaratinguetá, São Paulo, Brazil.
authors.forename: E V
authors.initials: EV
authors.lastname: Canettieri
authors.affiliation: Departamento de Engenharia, Universidade Estadual Paulista "Júlio de Mesquita Filho", 12516-410 Guaratinguetá, São Paulo, Brazil.
authors.forename: J A
authors.initials: JA
authors.lastname: Carvalho
authors.affiliation: Laboratório Associado de Combustão e Propulsão, Instituto Nacional de Pesquisas Espaciais, 12630-970 Cachoeira Paulista, São Paulo, Brazil.
authors.forename: T G
authors.initials: TG
authors.lastname: Soares Neto
authors.affiliation: Departamento de Biotecnologia, Escola de Engenharia de Lorena, Universidade de São Paulo, 12602-810 Lorena, São Paulo, Brazil.
authors.forename: M G A
authors.initials: MG
authors.lastname: Felipe
mesh_terms: D001709:Biotechnology / Q000379:methods*; D001938:Brazil; D002175:Candida / Q000378:metabolism*; D002475:Cellobiose / Q000378:metabolism; D005285:Fermentation; D005990:Glycerol / Q000378:metabolism; D006868:Hydrolysis; D008320:Maltose / Q000378:metabolism; D018520:Plant Shoots / Q000737:chemistry / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry*; D031786:Saccharum / Q000737:chemistry* / Q000378:metabolism; D013395:Sucrose / Q000378:metabolism; D014993:Xylitol / Q000096:biosynthesis*; D014994:Xylose / Q000378:metabolism
chemical_list: D011134:Polysaccharides; D002475:Cellobiose; D013395:Sucrose; D008320:Maltose; C007916:hemicellulose; D014994:Xylose; D005990:Glycerol; D014993:Xylitol
keywords: Candida guilliermondii; Co-substrates; Hemicellulosic hydrolyzate; Sugarcane straw; Xylitol