title: Anti-angiogenic activities of CRBGP from buccal glands of lampreys (Lampetra japonica).
abstract: Cysteine-rich secretory proteins (CRISPs), characterized by 16 conserved cysteines, are distributed in a wide range of organisms, such as secernenteas, amphibians, reptiles and mammals. In the previous studies, a novel CRISP family member (cysteine-rich buccal gland protein, CRBGP) was separated from the buccal gland of lampreys (Lampetra japonica, L. japonica). Lamprey CRBGP could not only suppress depolarization-induced contraction of rat tail arterial smooth muscle, but also block voltage-gated sodium channels (VGSCs). In the present study, the anti-angiogenic activities of lamprey CRBGP were investigated using endothelial cells and chick chorioallantoic membrane (CAM) models. In vitro assays, lamprey CRBGP is able to induce human umbilical vein endothelial cells (HUVECs) apoptosis by disturbing the calcium homeostasis and mitochondria functions. In addition, lamprey CRBGP could inhibit proliferation, adhesion, migration, invasion and tube formation of HUVECs by affecting the organization of F-actin and expression level of matrix metallo-proteinase 2 (MMP-2), matrix metallo-proteinase 9 (MMP-9) and vascular endothelial growth factor A (VEGFA) which are related to angiogenesis. In vivo assays, lamprey CRBGP could suppress the blood vessel formation in CAM models. Therefore, lamprey CRBGP is an important protein present in the buccal gland of lampreys and might help lampreys suppress the contraction of blood vessels, nociceptive responses and wound healing of host fishes during their feeding time. In addition, lamprey CRBGP might have the potential to act as an effective anti-angiogenic factor for the treatment of abnormal angiogenesis induced diseases.
authors.affiliation: School of Life Sciences, Liaoning Normal University, Dalian 116081, PR China; Lamprey Research Center, Liaoning Normal University, Dalian 116081, PR China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Jiang
authors.affiliation: School of Life Sciences, Liaoning Normal University, Dalian 116081, PR China; Lamprey Research Center, Liaoning Normal University, Dalian 116081, PR China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Liu
authors.affiliation: School of Life Sciences, Liaoning Normal University, Dalian 116081, PR China; Lamprey Research Center, Liaoning Normal University, Dalian 116081, PR China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Duan
authors.affiliation: School of Life Sciences, Liaoning Normal University, Dalian 116081, PR China; Lamprey Research Center, Liaoning Normal University, Dalian 116081, PR China.
authors.forename: Meng
authors.initials: M
authors.lastname: Gou
authors.affiliation: School of Life Sciences, Liaoning Normal University, Dalian 116081, PR China; Lamprey Research Center, Liaoning Normal University, Dalian 116081, PR China.
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
authors.affiliation: School of Life Sciences, Liaoning Normal University, Dalian 116081, PR China; Lamprey Research Center, Liaoning Normal University, Dalian 116081, PR China.
authors.forename: Jihong
authors.initials: J
authors.lastname: Wang
authors.affiliation: School of Life Sciences, Liaoning Normal University, Dalian 116081, PR China; Lamprey Research Center, Liaoning Normal University, Dalian 116081, PR China. Electronic address: liqw@263.net.
authors.forename: Qingwei
authors.initials: Q
authors.lastname: Li
authors.affiliation: School of Life Sciences, Liaoning Normal University, Dalian 116081, PR China; Lamprey Research Center, Liaoning Normal University, Dalian 116081, PR China. Electronic address: liulangmao1980@126.com.
authors.forename: Rong
authors.initials: R
authors.lastname: Xiao
mesh_terms: D020533:Angiogenesis Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D017209:Apoptosis; D002610:Cheek; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D007798:Lampreys; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D008565:Membrane Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D020533:Angiogenesis Inhibitors; D008565:Membrane Proteins; D042461:Vascular Endothelial Growth Factor A; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9
keywords: Anti-angiogenesis; Buccal gland; CRBGP; Lamprey