title: Enzyme-guided DNA Sewing Architecture.
abstract: With the advent of nanotechnology, a variety of nanoarchitectures with varied physicochemical properties have been designed. Owing to the unique characteristics, DNAs have been used as a functional building block for novel nanoarchitecture. In particular, a self-assembly of long DNA molecules via a piece DNA staple has been utilized to attain such constructs. However, it needs many talented prerequisites (e.g., complicated computer program) with fewer yields of products. In addition, it has many limitations to overcome: for instance, (i) thermal instability under moderate environments and (ii) restraint in size caused by the restricted length of scaffold strands. Alternatively, the enzymatic sewing linkage of short DNA blocks is simply designed into long DNA assemblies but it is more error-prone due to the undeveloped sequence data. Here, we present, for the first time, a comprehensive study for directly combining DNA structures into higher DNA sewing constructs through the 5'-end cohesive ligation of T4 enzyme. Inspired by these achievements, the synthesized DNA nanomaterials were also utilized for effective detection and real-time diagnosis of cancer-specific and cytosolic RNA markers. This generalized protocol for generic DNA sewing is expected to be useful in several DNA nanotechnology as well as any nucleic acid-related fields.
authors.affiliation: School of Chemical Engineering, Sungkyunkwan University, Suwon, Gyeonggi-do, 440-746, South Korea.
authors.forename: In Hyun
authors.initials: IH
authors.lastname: Song
authors.affiliation: School of Chemical Engineering, Sungkyunkwan University, Suwon, Gyeonggi-do, 440-746, South Korea.
authors.forename: Seung Won
authors.initials: SW
authors.lastname: Shin
authors.affiliation: School of Chemical Engineering, Sungkyunkwan University, Suwon, Gyeonggi-do, 440-746, South Korea.
authors.forename: Kyung Soo
authors.initials: KS
authors.lastname: Park
authors.affiliation: GREMAN, UMR7347, Université François Rabelais, 37200, France.
authors.forename: Yves
authors.initials: Y
authors.lastname: Lansac
authors.affiliation: School of Materials Science and Engineering, Gwangju Institute of Science and Technology, Gwangju, 61005, South Korea.
authors.forename: Yun Hee
authors.initials: YH
authors.lastname: Jang
authors.affiliation: School of Chemical Engineering, Sungkyunkwan University, Suwon, Gyeonggi-do, 440-746, South Korea.|SKKU Advanced Institute of Nanotechnology (SAINT), Sungkyunkwan University, Suwon, Gyeonggi-do, 440-746, South Korea.
authors.forename: Soong Ho
authors.initials: SH
authors.lastname: Um
mesh_terms: D015374:Biosensing Techniques; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D011088:DNA Ligases / Q000737:chemistry; D006801:Humans; D049329:Nanostructures / Q000737:chemistry*; D036103:Nanotechnology; D009690:Nucleic Acid Conformation; D012313:RNA / Q000737:chemistry
chemical_list: D012313:RNA; D004247:DNA; D011088:DNA Ligases