title: MiR-631/ZAP70: A novel axis in the migration and invasion of prostate cancer cells.
abstract: MicroRNAs (miRNAs) are short, endogenous non-coding RNA molecules involved in cancer initiation and progression. Using transwell migration and invasion assays, we found that miR-631 inhibited the migration and invasion of prostate cancer (PCa) cells. Bioinformatic algorithms indicated the 3'-untranslated region (3'-UTR) of zeta-associated protein 70 (ZAP70) has a putative binding site for miR-631. We found that miR-631 can bind to the 3'-UTR of ZAP70 and decrease its expression. Further studies confirmed that ZAP70 facilitates PCa cell migration and invasion. Interestingly, using gain- and loss-of function experiments, we found that ZAP70 is a major target of miR-631 and largely mediates its activity. In addition, we further discovered that miR-631 was downregulated and ZAP70 was overexpressed in PCa cell lines and PCa tissues. A concordant inverse correlation between miR-631 and ZAP70 was also found in PCa tissues. In all, our study demonstrates that miR-631 decreases PCa cell migration and invasion by dampening ZAP70 expression.
authors.affiliation: The Department of Urology Sugery, The First Affiliate Hospital of Liaoning Medical University, Jinzhou, Liaoning 121000, China. Electronic address: shenzhirui1@163.com.
authors.forename: Dewang
authors.initials: D
authors.lastname: Fu
authors.affiliation: The Department of Urology Sugery, The First Affiliate Hospital of Liaoning Medical University, Jinzhou, Liaoning 121000, China. Electronic address: liuben1013@163.com.
authors.forename: Ben
authors.initials: B
authors.lastname: Liu
authors.affiliation: The Department of Urology Sugery, The First Affiliate Hospital of Liaoning Medical University, Jinzhou, Liaoning 121000, China. Electronic address: zanglie1013@163.com.
authors.forename: Li E
authors.initials: LE
authors.lastname: Zang
authors.affiliation: The Department of Urology Sugery, The First Affiliate Hospital of Liaoning Medical University, Jinzhou, Liaoning 121000, China. Electronic address: lyjianghm@163.com.
authors.forename: Huamao
authors.initials: H
authors.lastname: Jiang
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement; D002470:Cell Survival; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000473:pathology*; D015398:Signal Transduction; D051746:ZAP-70 Protein-Tyrosine Kinase / Q000378:metabolism*
chemical_list: C000607908:MIRN631 microRNA, human; D035683:MicroRNAs; D051746:ZAP-70 Protein-Tyrosine Kinase; C497261:ZAP70 protein, human
keywords: Invasion; MicroRNA-631; Migration; Prostate cancer; ZAP70