title: The fusion of autophagosome with lysosome is impaired in L-arginine-induced acute pancreatitis.
abstract: OBJECTIVE
  Acute pancreatitis is an inflammatory pancreatic disease that carries considerable morbidity and mortality. The pathogenesis of this disease remains poorly understood. We investigated the incidence of autophagy in mice following induction of acute pancreatitis.
  
  
  METHODS
  Mice were received intraperitoneal injections of L-arginine (200 mg × 2/100 g BW), while controls were administered with saline. Pancreatic tissues were assessed by histology, electron microscopy and western blotting.
  
  
  RESULTS
  Injection of L-arginine resulted in the accumulation of autophagosomes and a relative paucity of autolysosomes. Moreover, the autophagy marker p62 is significantly increased. However, the lysosomal-associated membrane protein-2 (Lamp-2), a protein that is required for the proper fusion of autophagosomes with lysosomes, is decreased in acute pancreatitis. These results suggest that a crucial role for autophagy and Lamp-2 in the pathogenesis of acute pancreatitis.
  
  
  CONCLUSIONS
  Our data suggest that the autophagic flux is impaired in acute pancreatitis. The depletion of Lamp-2 may play a role in the pathogenesis of acute pancreatitis.
authors.affiliation: Department of Gastrointestinal and Pancreatic Surgery, Third Xiangya Hospital, Central South University Changsha 410013, Hunan, China.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Zhu
authors.affiliation: Department of Gastrointestinal and Pancreatic Surgery, Third Xiangya Hospital, Central South University Changsha 410013, Hunan, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Yu
authors.affiliation: Department of Gastrointestinal and Pancreatic Surgery, Third Xiangya Hospital, Central South University Changsha 410013, Hunan, China.
authors.forename: Shaihong
authors.initials: S
authors.lastname: Zhu
authors.affiliation: Department of Endocrinology, Third Xiangya Hospital, Central South University Changsha 410013, Hunan, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Hematology, Third Xiangya Hospital, Central South University Changsha 410013, Hunan, China.
authors.forename: Ben
authors.initials: B
authors.lastname: Lu
authors.affiliation: Department of Gastrointestinal and Pancreatic Surgery, Third Xiangya Hospital, Central South University Changsha 410013, Hunan, China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Gastrointestinal and Pancreatic Surgery, Third Xiangya Hospital, Central South University Changsha 410013, Hunan, China.
authors.forename: Can
authors.initials: C
authors.lastname: Yu
mesh_terms: D000208:Acute Disease; D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism; D000818:Animals; D001120:Arginine; D001343:Autophagy; D015415:Biomarkers / Q000378:metabolism; D015153:Blotting, Western; D004195:Disease Models, Animal; D006360:Heat-Shock Proteins / Q000378:metabolism; D052119:Lysosomal-Associated Membrane Protein 2 / Q000378:metabolism; D008247:Lysosomes / Q000378:metabolism / Q000473:pathology* / Q000648:ultrastructure; D008297:Male; D008561:Membrane Fusion; D051379:Mice; D008855:Microscopy, Electron, Scanning; D010179:Pancreas / Q000378:metabolism / Q000473:pathology* / Q000648:ultrastructure; D010195:Pancreatitis / Q000139:chemically induced* / Q000378:metabolism / Q000473:pathology*; D000071456:Sequestosome-1 Protein
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D015415:Biomarkers; D006360:Heat-Shock Proteins; D052119:Lysosomal-Associated Membrane Protein 2; D000071456:Sequestosome-1 Protein; C101343:Sqstm1 protein, mouse; D001120:Arginine
keywords: Acute pancreatitis; L-arginine; Lamp-2; autophagy; p62