title: Inhibitory effects of Na7PMo11CuO40 on mushroom tyrosinase and melanin formation and its antimicrobial activities.
abstract: Keggin-type Cu-substituted phosphomolybdic acid (Na7PMo11CuO40, abbreviated as PMo11Cu) was synthesized and characterized. The inhibitory effects of PMo11Cu on mushroom tyrosinase and melanin formation in B16 melanoma cells were studied. The results showed that PMo11Cu could strongly inhibit the activity of tyrosinase, and it was reversible and competitive inhibitor. The IC50 value was estimated to be 0.48 mM for diphenolase activity. PMo11Cu also exhibited inhibitory effects on cell viability, cellular tyrosinase activity and melanin formation in B16 melanoma cells at concentrations ranging from 0 to 200 μM for 24 h. Furthermore, the antimicrobial activities of PMo11Cu against Sarcina lutea, Staphylococcus aureus, Bacillus subtilis and Escherichia coli were investigated. The results showed that PMo11Cu had an obvious antimicrobial activities, and it was more effective against two kinds of coccus than two kinds of bacillus. This study may provide theoretical basis for designing novel effective mushroom tyrosinase inhibitors and extend the use of polyoxometalates in the fields of food preservation and depigmentation.
authors.affiliation: College of Food and Bioengineering, Jimei University, Xiamen 361021, PR China.
authors.forename: Rui
authors.initials: R
authors.lastname: Xing
authors.affiliation: College of Chemistry and Life Science, Quanzhou Normal College, Quanzhou 362000, PR China. Electronic address: dwf320@163.com.
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.affiliation: College of Chemistry and Life Science, Quanzhou Normal College, Quanzhou 362000, PR China.
authors.forename: Le
authors.initials: L
authors.lastname: Dong
authors.affiliation: College of Food and Bioengineering, Jimei University, Xiamen 361021, PR China.
authors.forename: A-Ping
authors.initials: AP
authors.lastname: Zheng
authors.affiliation: College of Food and Bioengineering, Jimei University, Xiamen 361021, PR China. Electronic address: wanglimerry@jmu.edu.cn.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.affiliation: College of Food and Bioengineering, Jimei University, Xiamen 361021, PR China.
authors.forename: Wen-Jin
authors.initials: WJ
authors.lastname: Su
authors.affiliation: College of Chemistry and Life Science, Quanzhou Normal College, Quanzhou 362000, PR China.
authors.forename: Ting
authors.initials: T
authors.lastname: Lin
mesh_terms: D000363:Agaricales / Q000201:enzymology*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D005519:Food Preservation; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D008543:Melanins / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D008546:Melanoma, Experimental / Q000378:metabolism / Q000473:pathology; D008826:Microbial Sensitivity Tests; D008982:Molybdenum / Q000737:chemistry / Q000494:pharmacology*; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors*; D010756:Phosphoric Acids / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D004791:Enzyme Inhibitors; D008543:Melanins; C000609180:Na7PMo11CuO40; D010756:Phosphoric Acids; D008982:Molybdenum; D014442:Monophenol Monooxygenase
keywords: Antimicrobial activity; Melanin formation; Polyoxometalates; Tyrosinase inhibition