title: Decellularization and Recellularization of Rat Livers With Hepatocytes and Endothelial Progenitor Cells.
abstract: Whole-organ decellularization has been identified as a promising choice for tissue engineering. The aim of the present study was to engineer intact whole rat liver scaffolds and repopulate them with hepatocytes and endothelial progenitor cells (EPCs) in a bioreactor. Decellularized liver scaffolds were obtained by perfusing Triton X-100 with ammonium hydroxide. The architecture and composition of the original extracellular matrix were preserved, as confirmed by morphologic, histological, and immunolabeling methods. To determine biocompatibility, the scaffold was embedded in the subcutaneous adipose layer of the back of a heterologous animal to observe the infiltration of inflammatory cells. Hepatocytes were reseeded using a parenchymal injection method and cultured by continuous perfusion. EPCs were reseeded using a portal vein infusion method. Morphologic and functional examination showed that the hepatocytes and EPCs grew well in the scaffold. The present study describes an effective method of decellularization and recellularization of rat livers, providing the foundation for liver engineering and the development of bioartificial livers.
authors.affiliation: Department of General Surgery, Affiliated Hospital of Nantong University, Nantong, China.|Department of Emergency Surgery, Affiliated Hospital of Nantong University, Nantong, China.
authors.forename: Pengcheng
authors.initials: P
authors.lastname: Zhou
authors.affiliation: Department of General Surgery, Affiliated Hospital of Nantong University, Nantong, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Surgical Comprehensive Laboratory, Affiliated Hospital of Nantong University, Nantong, China.
authors.forename: Yibing
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Department of General Surgery, Affiliated Hospital of Nantong University, Nantong, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of General Surgery, Affiliated Hospital of Nantong University, Nantong, China.
authors.forename: Changchun
authors.initials: C
authors.lastname: Ling
authors.affiliation: Department of General Surgery, Affiliated Hospital of Nantong University, Nantong, China.
authors.forename: Qingsong
authors.initials: Q
authors.lastname: Guo
authors.affiliation: Department of General Surgery, Affiliated Hospital of Nantong University, Nantong, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of General Surgery, Affiliated Hospital of Nantong University, Nantong, China.
authors.forename: Shajun
authors.initials: S
authors.lastname: Zhu
authors.affiliation: Department of General Surgery, Affiliated Hospital of Nantong University, Nantong, China.
authors.forename: Xiangjun
authors.initials: X
authors.lastname: Fan
authors.affiliation: Department of General Surgery, Affiliated Hospital of Nantong University, Nantong, China.
authors.forename: Mingyan
authors.initials: M
authors.lastname: Zhu
authors.affiliation: Department of Pathology, Affiliated Hospital of Nantong University, Nantong, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Huang
authors.affiliation: Department of General Surgery, Affiliated Hospital of Nantong University, Nantong, China.|Surgical Comprehensive Laboratory, Affiliated Hospital of Nantong University, Nantong, China.
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Department of General Surgery, Affiliated Hospital of Nantong University, Nantong, China.
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000818:Animals; D019149:Bioreactors; D049109:Cell Proliferation; D002478:Cells, Cultured; D066026:Endothelial Progenitor Cells / Q000166:cytology / Q000637:transplantation*; D005109:Extracellular Matrix / Q000737:chemistry*; D022781:Hepatocytes / Q000166:cytology / Q000637:transplantation*; D008099:Liver / Q000737:chemistry / Q000166:cytology* / Q000648:ultrastructure; D008810:Mice, Inbred C57BL; D051381:Rats; D017207:Rats, Sprague-Dawley; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
keywords: Decellularization; Endothelial progenitor cells; Extracellular matrix; Liver; Recellularization; Tissue Engineering