title: Synthesis of the biotinylated anti-HIV compound BMMP and the target identification study.
abstract: BMMP [2-(benzothiazol-2-ylmethylthio)-4-methylpyrimidine], an inhibitor of HIV-1 replication, was linked to biotin to study the interaction with the presumed target, HIV-1 Pr55(Gag) or CA, by means of surface plasmon resonance. The synthesized Biotin-BMMP inhibited HIV-1 replication to a similar extent as BMMP alone, but did not interact with Pr55(Gag) or CA, suggesting that BMMP exerts its activity by a different mechanism.
authors.affiliation: Department of Bioorganic Medicinal Chemistry, Faculty of Life Sciences, Kumamoto University, 5-1 Oe-honmachi, Chuo-ku, Kumamoto 862-0973, Japan. Electronic address: 141y1023@st.kumamoto-u.ac.jp.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Kamo
authors.affiliation: Department of Bioorganic Medicinal Chemistry, Faculty of Life Sciences, Kumamoto University, 5-1 Oe-honmachi, Chuo-ku, Kumamoto 862-0973, Japan. Electronic address: 155y2008@st.kumamoto-u.ac.jp.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tateishi
authors.affiliation: Department of Bioorganic Medicinal Chemistry, Faculty of Life Sciences, Kumamoto University, 5-1 Oe-honmachi, Chuo-ku, Kumamoto 862-0973, Japan. Electronic address: kk1205@kumamoto-u.ac.jp.
authors.forename: Ryoko
authors.initials: R
authors.lastname: Koga
authors.affiliation: Department of Bioorganic Medicinal Chemistry, Faculty of Life Sciences, Kumamoto University, 5-1 Oe-honmachi, Chuo-ku, Kumamoto 862-0973, Japan. Electronic address: okamoto@gpo.kumamoto-u.ac.jp.
authors.forename: Yoshinari
authors.initials: Y
authors.lastname: Okamoto
authors.affiliation: Department of Bioorganic Medicinal Chemistry, Faculty of Life Sciences, Kumamoto University, 5-1 Oe-honmachi, Chuo-ku, Kumamoto 862-0973, Japan. Electronic address: motsuka@gpo.kumamoto-u.ac.jp.
authors.forename: Masami
authors.initials: M
authors.lastname: Otsuka
authors.affiliation: Research Institute for Drug Discovery, School of Pharmacy, Kumamoto University, 5-1 Oe-honmachi, Chuo-ku, Kumamoto 862-0973, Japan. Electronic address: mfujita@kumamoto-u.ac.jp.
authors.forename: Mikako
authors.initials: M
authors.lastname: Fujita
mesh_terms: D019380:Anti-HIV Agents / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D052160:Benzothiazoles / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D019921:Biotinylation; D004305:Dose-Response Relationship, Drug; D015497:HIV-1 / Q000187:drug effects*; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D011743:Pyrimidines / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D014779:Virus Replication / Q000187:drug effects
chemical_list: C000609210:2-(benzothiazol-2-ylmethylthio)-4-methylpyrimidine; D019380:Anti-HIV Agents; D052160:Benzothiazoles; D011743:Pyrimidines
keywords: BMMP; Biotin; CA; HIV-1; Pr55(Gag)