title: Novel remodeling of the mouse heart mitochondrial proteome in response to acute insulin stimulation.
abstract: OBJECTIVE
  Mitochondrial dysfunction contributes to the pathophysiology of diabetic cardiomyopathy. The aim of this study was to investigate the acute changes in the mitochondrial proteome in response to insulin stimulation.
  
  
  RESULTS
  Cardiac mitochondria from C57BL/6 mice after insulin stimulation were analyzed using two-dimensional fluorescence difference gel electrophoresis. MALDI-TOF MS/MS was utilized to identify differences. Two enzymes involved in metabolism and four structural proteins were identified. Succinyl-CoA ligase [ADP forming] subunit beta was identified as one of the differentially regulated proteins. Upon insulin stimulation, a relatively more acidic isoform of this protein was increased by 53% and its functional activity was decreased by ∼32%.
  
  
  CONCLUSIONS
  This proteomic remodeling in response to insulin stimulation may play an important role in the normal and diabetic heart.
authors.affiliation: UC Irvine Diabetes Center, University of California at Irvine, Irvine, CA 92697, USA; Sue and Bill Gross Stem Cell Research Center, University of California at Irvine, Irvine, CA 92697, USA; Department of Medicine, University of California at Irvine, Irvine, CA 92697, USA; Department of Medicine, University of California at San Diego, La Jolla, CA 92093, USA.
authors.forename: B A
authors.initials: BA
authors.lastname: Pedersen
authors.affiliation: UC Irvine Diabetes Center, University of California at Irvine, Irvine, CA 92697, USA; Sue and Bill Gross Stem Cell Research Center, University of California at Irvine, Irvine, CA 92697, USA; Department of Medicine, University of California at Irvine, Irvine, CA 92697, USA; Systomic Health LLC, Los Angeles, CA 90039, USA.
authors.forename: P G
authors.initials: PG
authors.lastname: Yazdi
authors.affiliation: UC Irvine Diabetes Center, University of California at Irvine, Irvine, CA 92697, USA; Sue and Bill Gross Stem Cell Research Center, University of California at Irvine, Irvine, CA 92697, USA; Department of Medicine, University of California at Irvine, Irvine, CA 92697, USA.
authors.forename: J F
authors.initials: JF
authors.lastname: Taylor
authors.affiliation: UC Irvine Diabetes Center, University of California at Irvine, Irvine, CA 92697, USA; Sue and Bill Gross Stem Cell Research Center, University of California at Irvine, Irvine, CA 92697, USA.
authors.forename: O S
authors.initials: OS
authors.lastname: Khattab
authors.affiliation: UC Irvine Diabetes Center, University of California at Irvine, Irvine, CA 92697, USA; Sue and Bill Gross Stem Cell Research Center, University of California at Irvine, Irvine, CA 92697, USA; Department of Medicine, University of California at Irvine, Irvine, CA 92697, USA.
authors.forename: Y-H
authors.initials: YH
authors.lastname: Chen
authors.affiliation: UC Irvine Diabetes Center, University of California at Irvine, Irvine, CA 92697, USA; Sue and Bill Gross Stem Cell Research Center, University of California at Irvine, Irvine, CA 92697, USA; Department of Medicine, University of California at Irvine, Irvine, CA 92697, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Chen
authors.affiliation: UC Irvine Diabetes Center, University of California at Irvine, Irvine, CA 92697, USA; Sue and Bill Gross Stem Cell Research Center, University of California at Irvine, Irvine, CA 92697, USA; Department of Medicine, University of California at Irvine, Irvine, CA 92697, USA; Department of Biological Chemistry, University of California at Irvine, Irvine, CA 92697, USA; Department of Physiology & Biophysics, University of California at Irvine, Irvine, CA 92697, USA. Electronic address: phwang@uci.edu.
authors.forename: P H
authors.initials: PH
authors.lastname: Wang
mesh_terms: D000214:Acyl Coenzyme A / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D015153:Blotting, Western; D058065:Diabetic Cardiomyopathies / Q000378:metabolism / Q000503:physiopathology*; D004195:Disease Models, Animal; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D007274:Injections, Intraperitoneal; D007328:Insulin / Q000494:pharmacology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008929:Mitochondria, Heart / Q000187:drug effects* / Q000502:physiology*; D020543:Proteome / Q000187:drug effects*; D011897:Random Allocation; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods; D020858:Tissue and Organ Harvesting; D020257:Ventricular Remodeling / Q000502:physiology
chemical_list: D000214:Acyl Coenzyme A; D007328:Insulin; D020543:Proteome; C012046:succinyl-coenzyme A
keywords: DIGE; Insulin; Mitochondria; Proteomics; Succinyl-CoA ligase