title: Genetic Interaction Scoring Procedure for Bacterial Species.
abstract: A genetic interaction occurs when the phenotype of an organism carrying two mutant genes differs from what should have been observed given their independent influence. Such unexpected outcome indicates a mechanistic connection between the perturbed genes, providing a key source of functional information about the cell. Large-scale screening for genetic interactions involves measuring phenotypes of single and double mutants, which for microorganisms is usually done by automated analysis of images of ordered colonies. Obtaining accurate colony sizes, and using them to identify genetic interactions from such screens remains a challenging and time-consuming task. Here, we outline steps to compute genetic interaction scores in E. coli by measuring colony sizes from plate images, performing normalisation, and quantifying the strength of the effect.
authors.affiliation: EMBL-EBI (South Building), Wellcome Trust Genome Campus, Saffron Walden, CB10 1SD, UK.
authors.forename: Omar
authors.initials: O
authors.lastname: Wagih
authors.affiliation: EMBL-EBI (South Building), Wellcome Trust Genome Campus, Saffron Walden, CB10 1SD, UK.
authors.forename: Leopold
authors.initials: L
authors.lastname: Parts
mesh_terms: D001419:Bacteria / Q000235:genetics*; D011786:Quality Control
keywords: Colony; Genetic interaction; Image analysis; Normalisation; eSGA