title: Immunohistochemical Evaluation of Angiogenesis Related Markers in Pyogenic Granuloma of Gingiva.
abstract: BACKGROUND
  Pyogenic granuloma is a common non-neoplastic connective tissue proliferation. ICAM-1 and VCAM-1 are vascular adhesion molecules and CD34 is a marker for evaluation of angiogenesis. The purpose of this study was to compare the immunohistochemical expression of ICAM-1, VCAM-1 and CD34 in oral pyogenic granuloma and normal gingiva.
  
  
  METHODS
  This study was performed on thirty five formalin-fixed, paraffin embedded samples of gingival pyogenic granuloma. Also we used thirty five paraffined blocks of normal gingiva as control group which were taken from crown lengthening surgery. We employed immunohistochemistry staining for our prepared microscopic slides using monoclonal mouse anti-human antibodies against ICAM-1 (CD54), VCAM-1 (CD106) and CD34. Slides were examined under light microscope and then the mean amount of stained vessels also known as microvascular density (MVD) in highly vascularized areas (hot spots) was measured. Paired t-test and repeated measures ANOVA were used to compare the difference between quantitative variables and Chi-square test for qualitative variables in different groups. Pearson correlation coefficient was used to compare relations between quantitative variables. P<0.05 was considered significant.
  
  
  RESULTS
  The mean of MVD for ICAM-1, VCAM-1 and CD34 was significantly higher in pyogenic granuloma than normal gingiva (p<0.001 and p<0.001 and p<0.001, respectively). Expression of CD34 in pyogenic granuloma was significantly higher than ICAM-1 and VCAM-1 (P<0.001). Besides, expression of ICAM-1 in normal gingiva, was significantly lower than two other markers (p<0.001).
  
  
  CONCLUSIONS
  Regarding the results, it seems that ICAM-1, VCAM-1 and CD34 are useful biomarkers in evaluation of vascular and inflammatory lesions such as gingival pyogenic granuloma and the results indicate the role of these biomarkers in pathogenesis of oral pyogenic granuloma.
authors.affiliation: Dental Materials Research Center, Dental Faculty, Babol University of Medical Sciences, Babol, Iran E-mail : hashemipoorgolnar2@gmail.com.
authors.forename: Maryam
authors.initials: M
authors.lastname: Seyedmajidi
authors.forename: Shahryar
authors.initials: S
authors.lastname: Shafaee
authors.forename: Golnarsadat
authors.initials: G
authors.lastname: Hashemipour
authors.forename: Ali
authors.initials: A
authors.lastname: Bijani
authors.forename: Hodis
authors.initials: H
authors.lastname: Ehsani
mesh_terms: D000328:Adult; D018952:Antigens, CD34 / Q000378:metabolism*; D003430:Cross-Sectional Studies; D005260:Female; D005881:Gingiva / Q000098:blood supply* / Q000473:pathology*; D017789:Granuloma, Pyogenic / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D008297:Male; D009389:Neovascularization, Pathologic / Q000473:pathology*; D014021:Tissue Preservation; D019010:Vascular Cell Adhesion Molecule-1 / Q000378:metabolism*
chemical_list: D018952:Antigens, CD34; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1