title: Correlation between proinflammatory role of a lectin from Typhonium giganteum Engl. and macrophage.
abstract: OBJECTIVE
  To analyze the correlation between proinflammatory effects of a lectin from Typhonium giganteum Engl. and macrophage.
  
  
  METHODS
  T. giganteum lectin (TGL) was extracted from the tuber of T. giganteum and purified, and was then identified by using SDS-PAGE gel electrophoresis in combination with mass spectrometry. The morphologic changes of macrophage after being stimulated by TGL were observed with scanning electron microscopy. The influences of such stimulation on neutrophil migration were evaluated by establishing an in vitro macrophage-neutrophil co-culture migration model. By establishing a rat peritoneal macrophage in vitro cultured model, the effects of TGL stimulation on inflammatory factors TNF-α and IL-1β released by macrophage were analyzed. With p65 as the index, the expressions of the NF-κB signaling pathway in the cytoplasm and nucleus were detected before and after TGL stimulation respectively. Furthermore, we also investigated whether the inhibitor for NF-κB signaling pathway BAY11-7082 can block p65 nuclear translocation.
  
  
  RESULTS
  After being stimulated by TGL, macrophage had increased volume, number of pseudopodia and gradually cracked cell membrane, accompanied by evidently induced migration of neutrophils due to released inflammatory factors. As the concentration of TGL varied, NF-κB's monomer p65 had different expression levels in the cytoplasm and nucleus, while BAY11-7082 can indeed block the nuclear translocation of p65.
  
  
  CONCLUSIONS
  TGL-induced inflammation was closely related to macrophage mediation.
authors.affiliation: College of Pharmacology, Nanjing University of Chinese Medicine Nanjing 210023, China.
authors.forename: Yaozong
authors.initials: Y
authors.lastname: Pan
authors.affiliation: College of Pharmacology, Nanjing University of Chinese Medicine Nanjing 210023, China ; Jiangsu Key Laboratory of Chinese Medicine Processing Nanjing 210023, China ; Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing Nanjing 210023, China.
authors.forename: Hongli
authors.initials: H
authors.lastname: Yu
authors.affiliation: College of Pharmacology, Nanjing University of Chinese Medicine Nanjing 210023, China ; Jiangsu Key Laboratory of Chinese Medicine Processing Nanjing 210023, China ; Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing Nanjing 210023, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Wu
authors.affiliation: College of Pharmacology, Nanjing University of Chinese Medicine Nanjing 210023, China.
authors.forename: Yeqing
authors.initials: Y
authors.lastname: Chen
authors.affiliation: College of Pharmacology, Nanjing University of Chinese Medicine Nanjing 210023, China.
authors.forename: Kuilong
authors.initials: K
authors.lastname: Wang
authors.affiliation: College of Pharmacology, Nanjing University of Chinese Medicine Nanjing 210023, China.
authors.forename: Liping
authors.initials: L
authors.lastname: Liu
authors.affiliation: College of Pharmacology, Nanjing University of Chinese Medicine Nanjing 210023, China.
authors.forename: Yangping
authors.initials: Y
authors.lastname: Jin
authors.affiliation: College of Pharmacology, Nanjing University of Chinese Medicine Nanjing 210023, China.
authors.forename: Chengchao
authors.initials: C
authors.lastname: Zhang
mesh_terms: D000818:Animals; D029064:Araceae; D015153:Blotting, Western; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D002853:Chromatography, Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D007249:Inflammation / Q000378:metabolism*; D037102:Lectins / Q000494:pharmacology*; D017737:Macrophages, Peritoneal / Q000187:drug effects*; D013058:Mass Spectrometry; D008855:Microscopy, Electron, Scanning; D009504:Neutrophils / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D035281:Plant Tubers; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D037102:Lectins; D010936:Plant Extracts
keywords: NF-κB; Typhonium giganteum Engl. Lectin; macrophage