title: Overexpression of histone demethylase JMJD5 promotes metastasis and indicates a poor prognosis in breast cancer.
abstract: In this study, we showed the expression of JMJD5 was increased in breast cancer tissues and breast adenocarcinoma cell lines MCF-7 as well as triple negative breast cancer cell lines MDA-MB-231 compared with paired adjacent normal mammary tissues and normal mammary epithelial cell lines MCF-10A. The higher expression of JMJD5 was significantly corresponded with clinical stage, histological grade and lymph node metastasis. Overexpression of JMJD5 promoted cell invasion and induce EMT, while JMJD5 siRNA inhibits MDA-MB-231 cells invasion in vitro. Moreover, qChIP analysis revealed the Snail family proteins Snai1 was the direct target of JMJD5 in breast cancer cells. Luciferase reporter assays suggested that the overexpression of JMJD5 resulted in the activation of Snail1 promoter-driven luciferase reporter. The changes in the level of RNA and protein implied that the activation of Snail was the important mechanisms by which JMJD5 triggers metastasis. We also detected the higher expression of JMJD5 protein was an independent unfavorable biomarker for worse overall survival in breast cancer patients. Therefore, our results identified an important role for JMJD5 in breast cancer through the regulation of snail1.
authors.affiliation: Department of Nuclear Medicine, Qilu Hospital, Shandong University Wenhua Xi Road, Jinan 250012, Shandong Province, China ; Department of Nuclear Medicine, Weifang People's Hospital Weifang 261000, Shandong Province, China.
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: Department of Interventional Radiology, Weifang Peoples Hospital Weifang 261000, Shandong Province, China.
authors.forename: Chuntao
authors.initials: C
authors.lastname: Sun
authors.affiliation: Department of Nuclear Medicine, Weifang People's Hospital Weifang 261000, Shandong Province, China.
authors.forename: Fengqi
authors.initials: F
authors.lastname: Li
authors.affiliation: Department of Nuclear Medicine, Qilu Hospital, Shandong University Wenhua Xi Road, Jinan 250012, Shandong Province, China.
authors.forename: Jiankui
authors.initials: J
authors.lastname: Han
authors.affiliation: Department of Nuclear Medicine, Weifang People's Hospital Weifang 261000, Shandong Province, China.
authors.forename: Xanjun
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Nuclear Medicine, Weifang People's Hospital Weifang 261000, Shandong Province, China.
authors.forename: Zhenguo
authors.initials: Z
authors.lastname: Song
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D000328:Adult; D000368:Aged; D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D045744:Cell Line, Tumor; D005260:Female; D056466:Histone Demethylases / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008207:Lymphatic Metastasis / Q000235:genetics* / Q000473:pathology; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000235:genetics / Q000473:pathology; D011379:Prognosis; D011401:Promoter Regions, Genetic; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D064726:Triple Negative Breast Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D015854:Up-Regulation; D055815:Young Adult
chemical_list: C000604407:SNAI1 protein, human; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors; D056466:Histone Demethylases; C550342:KDM8 protein, human
keywords: JMJD5; biomarker; breast cancer; metastasis; snail