title: Analysis on the complete genome of a novel caprine parainfluenza virus 3.
abstract: Parainfluenza virus type 3 (PIV3) is one of the most important viral respiratory pathogens for humans and for many animals. One unique caprine PIV3 (CPIV3) strain named JS2013 was isolated in Chinese goat flocks with respiratory diseases in 2013. Now, the complete genome sequence of the strain JS2013 had been determined. A total of 15 overlapping DNA clones, covering the entire genome of the virus, were obtained by primer walking RT-PCR. The sequences of the 3' and 5' termini of the viral genome were amplified by 3' and 5' RACE. The viral genome was 15,618 nucleotides (nt) in length, which was consisted of six genes in the order 5'-leader-N-P/C/V-M-F-HN-L-tailer-3'. The junction sequences between two genes were highly conserved gene start and stop signal sequences, and trinucleotide intergenic regions (IGR) similar to those of other reported PIV3 strains. Phylogenetic analysis based on the complete genomes of JS2013 with other strains of genus Respirovirus demonstrated that the JS2013 obviously differed from HPIV1, Sendai virus, HPIV3 and other reported BPIV3 genotypes. Further analysis of HN genes of JS2013 along with two more CPIV3 strains isolated later indicated that CPIV3 strains formed a separate cluster. The results presented here suggested that CPIV3 is a new member of the genus Respirovirus.
authors.affiliation: Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China; Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing 210014, China; National Center for Engineering Research of Veterinary Bio-products, Nanjing 210014, China.
authors.forename: Leilei
authors.initials: L
authors.lastname: Yang
authors.affiliation: Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China; Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing 210014, China; National Center for Engineering Research of Veterinary Bio-products, Nanjing 210014, China.
authors.forename: Wenliang
authors.initials: W
authors.lastname: Li
authors.affiliation: Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China; Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing 210014, China; National Center for Engineering Research of Veterinary Bio-products, Nanjing 210014, China.
authors.forename: Li
authors.initials: L
authors.lastname: Mao
authors.affiliation: Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China; Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing 210014, China; National Center for Engineering Research of Veterinary Bio-products, Nanjing 210014, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Hao
authors.affiliation: Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China; Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing 210014, China; National Center for Engineering Research of Veterinary Bio-products, Nanjing 210014, China.
authors.forename: Zhongyu
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China; Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing 210014, China; National Center for Engineering Research of Veterinary Bio-products, Nanjing 210014, China.
authors.forename: Wenwen
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China; Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing 210014, China; National Center for Engineering Research of Veterinary Bio-products, Nanjing 210014, China.
authors.forename: Jiawu
authors.initials: J
authors.lastname: Deng
authors.affiliation: Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China; Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing 210014, China; National Center for Engineering Research of Veterinary Bio-products, Nanjing 210014, China. Electronic address: 1776556843@qq.com.
authors.forename: Jieyuan
authors.initials: J
authors.lastname: Jiang
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D005814:Genes, Viral; D016679:Genome, Viral; D023281:Genomics; D015511:Goat Diseases / Q000821:virology*; D006041:Goats; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D018108:Respirovirus / Q000145:classification* / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
keywords: Caprine parainfluenza virus 3 (CPIV3); Complete genome; ORF; Phylogenetic analysis