title: Distinctive immunostaining of claudin-4 in spiradenomas.
abstract: The intercellular bridges are essential structures in maintaining the histologic organization of the epithelium, while providing a very efficient way to exchange molecules between cells and transduction of the cell-to-cell and matrix-to-cell signals. Derangement in those important structures' physical integrity and/or function, which can be assessed by the presence or absence of several intercellular bridge proteins including claudin-4, E-cadherin, and β-catenin, was found to be related to several phenomena in the path to the neoplastic transformation. However, these proteins have not been studied in the wide variety of the skin neoplasms, in detail. Herein, we immunohistochemically assessed the expression patterns of these 3 intercellular bridge proteins on a total of 86 epidermal and eccrine adnexal tumors including basal cell carcinoma, squamous cell carcinoma, poroma, spiradenoma, syringoma, and hidradenoma. We observed a selective and distinct claudin-4 expression in the ductal-type cells of all cases of spiradenomas. Similarly, in the poromas, syringomas, and hidradenomas, claudin-4 was only positive in the luminal cells of microcystic structures, although not as conspicuous as in the spiradenomas. On the other hand, E-cadherin and β-catenin were positive in almost all types of the tumors, in a way which was not contributory to differentiate from each other. In conclusion, we think that claudin-4 can be helpful at least in making a reliable differential diagnosis of spiradenoma when overlapping morphologic features do not allow to further subclassification in the overwhelming variety of the adnexal tumors.
authors.affiliation: Department of Pathology, Gülhane Military Medical Academy and School of Medicine, Ankara, Turkey. Electronic address: nyigit@gata.edu.tr.
authors.forename: Nuri
authors.initials: N
authors.lastname: Yiğit
authors.affiliation: Department of Pathology, İzmir Military Hospital, İzmir, Turkey. Electronic address: ertugrul_celik@hotmail.com.
authors.forename: Ertuğrul
authors.initials: E
authors.lastname: Çelik
authors.affiliation: Department of Pathology, Gülhane Military Medical Academy and School of Medicine, Ankara, Turkey. Electronic address: ibrahimyavan@gmail.com.
authors.forename: İbrahim
authors.initials: İ
authors.lastname: Yavan
authors.affiliation: Department of Pathology, Gülhane Military Medical Academy and School of Medicine, Ankara, Turkey. Electronic address: agunal@gata.edu.tr.
authors.forename: Armağan
authors.initials: A
authors.lastname: Günal
authors.affiliation: Department of Pathology, Gülhane Military Medical Academy and School of Medicine, Ankara, Turkey. Electronic address: bkurt@gata.edu.tr.
authors.forename: Bülent
authors.initials: B
authors.lastname: Kurt
authors.affiliation: Department of Pathology, Gülhane Military Medical Academy and School of Medicine, Ankara, Turkey. Electronic address: ykarslioglu@gata.edu.tr.
authors.forename: Yıldırım
authors.initials: Y
authors.lastname: Karslıoğlu
authors.affiliation: Department of Pathology, Gülhane Military Medical Academy and School of Medicine, Ankara, Turkey. Electronic address: oonguru@gata.edu.tr.
authors.forename: Önder
authors.initials: Ö
authors.lastname: Öngürü
authors.affiliation: Department of Pathology, Gülhane Military Medical Academy and School of Medicine, Ankara, Turkey. Electronic address: aozcan@gata.edu.tr.
authors.forename: Ayhan
authors.initials: A
authors.lastname: Özcan
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis*; D062506:Claudin-4 / Q000032:analysis / Q000096:biosynthesis*; D006801:Humans; D007150:Immunohistochemistry; D018294:Neoplasms, Adnexal and Skin Appendage / Q000175:diagnosis* / Q000378:metabolism; D012189:Retrospective Studies; D012878:Skin Neoplasms / Q000175:diagnosis* / Q000378:metabolism
chemical_list: D014408:Biomarkers, Tumor; C568157:CLDN4 protein, human; D062506:Claudin-4
keywords: Claudin-4; Eccrine tumors; Intercellular bridge proteins; Skin tumors; Spiradenoma