title: Interferon-λ1 suppresses invasion and enhances autophagy in human osteosarcoma cell.
abstract: OBJECTIVE
  The purpose of the present study was to determine whether type III IFN can modulate the autophagic response in human osteosarcoma cell.
  
  
  METHODS
  Human osteosarcoma cell were treated with Interferon-λ1. We investigated that Interferon-λ1 could inhibit the invasive ability of osteosarcoma cells by Matrigel invasion assay. Autophagy were assessed by acridine orange staining, MDC staining and Transmission electron microscopy.
  
  
  RESULTS
  In this study, we found that Interferon-λ1 could inhibit the invasive ability of osteosarcoma cells. Acridine orange staining and MDC staining showed that Interferon-λ1 triggered the accumulation of acidic vesicular and autolysosomes in osteosarcoma cell. The acridine orange osteosarcoma cell ratios were 3.6 ± 0.5%, 4.5 ± 0.8%, and 12.4 ± 1.7% after treatment with 1, 10, and 100 ng/mL Interferon-λ1 for 48 h. Osteosarcoma cell cells treated with 100 ng/mL Interferon-λ1 for 48 h developed autophagy some-like characteristics, including single or double-membrane vacuoles containing intact and degraded cellular debris.
  
  
  CONCLUSIONS
  Interferon-λ1 could inhibit the invasive ability of osteosarcoma cells. Autophagy can be induced in a dose-dependent manner by treatment with Interferon-λ1 in osteosarcoma cell.
authors.affiliation: Department of Clinical Laboratory, Third Affiliated Hospital of Anhui Medical University Hefei 230032, China.
authors.forename: Dongmei
authors.initials: D
authors.lastname: Gao
authors.affiliation: Department of Microbiology, Anhui Medical University 69 Meishan Road, Hefei 230032, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Department of Clinical Laboratory, Third Affiliated Hospital of Anhui Medical University Hefei 230032, China.
authors.forename: Xiuyi
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Clinical Laboratory, Third Affiliated Hospital of Anhui Medical University Hefei 230032, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Xia
authors.affiliation: Department of Clinical Laboratory, Third Affiliated Hospital of Anhui Medical University Hefei 230032, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Cai
authors.affiliation: Department of Clinical Laboratory, Third Affiliated Hospital of Anhui Medical University Hefei 230032, China.
authors.forename: Jiwen
authors.initials: J
authors.lastname: Pan
authors.affiliation: Department of Clinical Laboratory, Third Affiliated Hospital of Anhui Medical University Hefei 230032, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Department of Clinical Laboratory, Third Affiliated Hospital of Anhui Medical University Hefei 230032, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Fang
authors.affiliation: Department of Clinical Laboratory, Third Affiliated Hospital of Anhui Medical University Hefei 230032, China.
authors.forename: Shouzhu
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Clinical Laboratory, Third Affiliated Hospital of Anhui Medical University Hefei 230032, China.
authors.forename: He
authors.initials: H
authors.lastname: Wen
keywords: Interferon-λ1; acridine orange; autophagy; transmission electron microscopy