title: Expression of vascular endothelial growth factor (VEGF) under hypoxia in placenta with intrahepatic cholestasis of pregnancy and its clinically pathological significance.
abstract: The expression of vascular endothelial growth factor (VEGF) under hypoxia in the placenta with intrahepatic cholestasis of pregnancy (ICP) was observed, and mechanisms of ICP fetal distress were discussed.
  
  
  METHODS
  Different culturing times were established in hypoxia incubator, and protein expressions of VEGF in placental tissue were observed using immunohistochemical S-P method.
  
  
  RESULTS
  After 4 h hypoxic culture, VEGF protein expression in ICP group was higher than the normal group with significant difference (P < 0.05). With the extension of hypoxic exposure, VEGF protein expression in both groups was suppressed, but no distinction in-between. Regression analyses indicated a noticeable effect of CG on VEGF expression, the higher the CG was, the lower the VEGF protein expression was (P < 0.05).
  
  
  CONCLUSIONS
  Short term hypoxia induces up-regulation of VEGF expression in ICP placenta, and this adaptive change is probably a protective mechanism of fetus in ICP.
authors.affiliation: Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Soochow University Suzhou, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Soochow University Suzhou, China.
authors.forename: Xiao-Hong
authors.initials: XH
authors.lastname: Pan
authors.affiliation: Department of Molecular Medicine Center, The Second Affiliated Hospital of Soochow University Suzhou, China.
authors.forename: Li
authors.initials: L
authors.lastname: Xiao
mesh_terms: D000328:Adult; D002780:Cholestasis, Intrahepatic / Q000378:metabolism*; D005260:Female; D006801:Humans; D000860:Hypoxia / Q000378:metabolism*; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D007231:Infant, Newborn; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D011248:Pregnancy Complications / Q000378:metabolism*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*
chemical_list: C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A
keywords: Vascular endothelial growth factor; hypoxia; immunohistochemistry; intrahepatic cholestasis of pregnancy; placenta