title: Rickettsia rickettsii isolation from naturally infected Amblyomma parvum ticks by centrifugation in a 24-well culture plate technique.
abstract: Rocky Mountain spotted fever is an acute illness caused by Rickettsia rickettsii (R. rickettsii) and is transmitted by the bite of ticks of the genera Dermacentor, Amblyomma and Rhipicephalus. The illness results in a high mortality rate and may be easily confused with other febrile syndromes. In Yucatan State, Mexico, childhood cases with a high mortality have been reported. In this work we report the isolation of a Mexican R. rickettsii strain from a tick egg mass using an alternative method for Rickettsia isolation with 24-well plates. We also identified a potential vector of R. rickettsii in the southeast of Mexico, which is Amblyomma parvum.
authors.affiliation: Regional Research Center "Dr. Hideyo Noguchi", Autonomous University of Yucatan, Mexico.
authors.forename: K
authors.initials: K
authors.lastname: Dzul-Rosado
authors.affiliation: Faculty of Medicine, Interinstitutional Unit of Clinical and Epidemiological Research. Autonomous University of Yucatan, Mexico.
authors.forename: G
authors.initials: G
authors.lastname: Peniche-Lara
authors.affiliation: Regional Research Center "Dr. Hideyo Noguchi", Autonomous University of Yucatan, Mexico.
authors.forename: R
authors.initials: R
authors.lastname: Tello-Martín
authors.affiliation: Faculty of Medicine, Interinstitutional Unit of Clinical and Epidemiological Research. Autonomous University of Yucatan, Mexico.
authors.forename: J
authors.initials: J
authors.lastname: Zavala-Velázquez
authors.affiliation: Federal University of Mato Grosso, Laboratory of Veterinary Parasitology, Department of Basic Science and animal production, Faculty of Agronomy, Animal Medicine and zootechny, Brazil.
authors.forename: R de Campos
authors.initials: Rde C
authors.lastname: Pacheco
authors.affiliation: Parasitic Disease laboratory, Department of Preventive Veterinary Medicine and Animal Health, Faculty of Veterinary Medicine and Zootechny, São Paulo University, São Paulo, SP, Brazil.
authors.forename: M B
authors.initials: MB
authors.lastname: Labruna
authors.affiliation: Government Health Services, Hospital Agustín O´Horan SSY, Calle Itzaes s/n. Col Centro, Merida Yucatan, Mexico.
authors.forename: E C
authors.initials: EC
authors.lastname: Sánchez
authors.affiliation: Regional Research Center "Dr. Hideyo Noguchi", Autonomous University of Yucatan, Mexico.
authors.forename: J
authors.initials: J
authors.lastname: Zavala-Castro
keywords: Amblyomma parvum; Rickettsia rickettsii; Rocky Mountain spotted fever