title: Effects of morphine and sufentanil preconditioning against myocardial ischemic-reperfusion injury in rabbits.
abstract: OBJECTIVE
  This study aims to explore the treatment method of myocardial ischemia-reperfusion injury.
  
  
  METHODS
  Myocardial Ischemia-reperfusion rabbit model was established in this study. They were divided into four groups: sham operation (S) group, IRI control (I/R) group and IRI with morphine (MF) group and sufentanil (SF). Myocardial infarct size was compared with HE staining method. TUNEL assay was used to detect cell apoptosis.
  
  
  RESULTS
  Myocardial infarct size of control group and morphine and sufetanil group was 36.0±3.6, 23.0±1.2 and 27.1±2.3, respectively. There were significant differences between them (P < 0.01). Apoptotic index of I/R, MF and SF groups was 26.9±2.2, 12.5±2.3, 15.8±2.0, with statistical significance (P < 0.05). The concentration of CK-MB in serum: there were no significant differences of CK-MB between each group at baseline. The concentration of CK-MB after reperfusion were higher than that of baseline, except for group S (P < 0.05); Compared with group S, after reperfusion, the CK-MB of other three groups were higher (P < 0.05); The concentration of CK-MB in group MF and SF were lower than group I/R (P < 0.05); In contrast to group MF, the concentration of CK-MB after reperfusion was higher in group SF (P < 0.05).
  
  
  CONCLUSIONS
  Morphine and sufentanil can specifically protect the myocardial function.
authors.affiliation: Department of Anesthesiology, Jiangxi Provincial People's Hospital Nanchang, China.
authors.forename: Xiu-Hong
authors.initials: XH
authors.lastname: Wang
authors.affiliation: Department of Anesthesiology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University Guangzhou, China.
authors.forename: Jian-Feng
authors.initials: JF
authors.lastname: Zeng
authors.affiliation: Department of Surgery, Jiangxi Cancer Hospital Nanchang, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Lin
authors.affiliation: Department of Anesthesiology, The First Affiliated Hospital of Nanchang University Nanchang, China.
authors.forename: Shi-Biao
authors.initials: SB
authors.lastname: Chen
keywords: Myocardial ischemia-reperfusion; morphine; sufentanil