title: Analytical performance of the Alere™ i Influenza A&B assay for the rapid detection of influenza viruses.
abstract: The analytical performance of the new Alere™ i Influenza A&B kit (AL-Flu) assay, based on isothermal nucleic acids amplification, was evaluated and compared with an antigen detection method, SD Bioline Influenza Virus Antigen Test (SDB), and an automated real-time RT-PCR, Simplexa™ Flu A/B & VRS Direct assay (SPX), for detection of influenza viruses. An "in-house" RT-PCR was used as the reference method. Sensitivity of AL-Flu, SDB, and SPX was 71.7%, 34.8%, and 100%, respectively. Specificity was 100% for all techniques. The turnaround time was 13min for AL-Flu, 15min for SDB, and 75min for SPX. The Alere™ i Influenza A&B assay is an optimal point-of-care assay for influenza diagnosis in clinical emergency settings, and is more sensitive and specific than antigen detection methods.
authors.affiliation: Servicio de Microbiología, Hospital Universitario Virgen de las Nieves, Instituto Biosanitario de Granada, Granada, Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: Riazzo
authors.affiliation: Servicio de Microbiología, Hospital Universitario Virgen de las Nieves, Instituto Biosanitario de Granada, Granada, Spain. Electronic address: mercedes.perez.ruiz.sspa@juntadeandalucia.es.
authors.forename: Mercedes
authors.initials: M
authors.lastname: Pérez-Ruiz
authors.affiliation: Servicio de Microbiología, Hospital Universitario Virgen de las Nieves, Instituto Biosanitario de Granada, Granada, Spain.
authors.forename: Sara
authors.initials: S
authors.lastname: Sanbonmatsu-Gámez
authors.affiliation: Servicio de Microbiología, Hospital Universitario Virgen de las Nieves, Instituto Biosanitario de Granada, Granada, Spain.
authors.forename: Irene
authors.initials: I
authors.lastname: Pedrosa-Corral
authors.affiliation: Servicio de Microbiología, Hospital Universitario Virgen de las Nieves, Instituto Biosanitario de Granada, Granada, Spain.
authors.forename: José
authors.initials: J
authors.lastname: Gutiérrez-Fernández
authors.affiliation: Servicio de Microbiología, Hospital Universitario Virgen de las Nieves, Instituto Biosanitario de Granada, Granada, Spain.
authors.forename: José-María
authors.initials: JM
authors.lastname: Navarro-Marí
mesh_terms: D006801:Humans; D009980:Influenza A virus / Q000302:isolation & purification*; D009981:Influenza B virus / Q000302:isolation & purification*; D007251:Influenza, Human / Q000175:diagnosis* / Q000821:virology*; D025202:Molecular Diagnostic Techniques; D021141:Nucleic Acid Amplification Techniques; D012189:Retrospective Studies; D013997:Time Factors
keywords: Detección rápida; Gripe; Influenza; Molecular testing; Método molecular; Rapid detection