title: Mapping of brain lipid binding protein (Blbp) in the brain of adult zebrafish, co-expression with aromatase B and links with proliferation.
abstract: Adult fish exhibit a strong neurogenic capacity due to the persistence of radial glial cells. In zebrafish, radial glial cells display well-established markers such as the estrogen-synthesizing enzyme (AroB) and the brain lipid binding protein (Blbp), which is known to strongly bind omega-3 polyunsaturated fatty acids such as docosahexaenoic acid (DHA). While Blpb is mainly described in the telencephalon of adult zebrafish, its expression in the remaining regions of the brain is poorly documented. The present study was designed to further investigate Blbp expression in the brain, its co-expression with AroB, and its link with radial glial cells proliferation in zebrafish. We generated a complete and detailed mapping of Blbp expression in the whole brain and show its complete co-expression with AroB, except in some tectal and hypothalamic regions. By performing PCNA and Blbp immunohistochemistry on cyp19a1b-GFP (AroB-GFP) fish, we also demonstrated preferential Blbp expression in proliferative radial glial cells in almost all regions studied. To our knowledge, this is the first complete and detailed mapping of Blbp-expressing cells showing strong association between Blbp and radial glial cell proliferation in the adult brain of fish. Given that zebrafish is now recognized models for studying neurogenesis and brain repair, our data provide detailed characterization of Blbp in the entire brain and open up a broad field of research investigating the role of omega-3 polyunsaturated fatty acids in neural stem cell activity in fish.
authors.affiliation: Inserm, UMR 1188 Diabète athérothrombose Thérapies Réunion Océan Indien (DéTROI), plateforme CYROI, Sainte-Clotilde, F-97490, France; Université de La Réunion, UMR 1188, Sainte-Clotilde, F-97490, France; Inserm U1085, Université de Rennes 1, Campus de Beaulieu, Research Institute in Health, Environment and Occupation, SFR Biosit, Rennes cedex, France. Electronic address: nicolas.diotel@univ-reunion.fr.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Diotel
authors.affiliation: Inserm U1085, Université de Rennes 1, Campus de Beaulieu, Research Institute in Health, Environment and Occupation, SFR Biosit, Rennes cedex, France.
authors.forename: Colette
authors.initials: C
authors.lastname: Vaillant
authors.affiliation: Inserm U1085, Université de Rennes 1, Campus de Beaulieu, Research Institute in Health, Environment and Occupation, SFR Biosit, Rennes cedex, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Kah
authors.affiliation: Inserm U1085, Université de Rennes 1, Campus de Beaulieu, Research Institute in Health, Environment and Occupation, SFR Biosit, Rennes cedex, France.
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Pellegrini
mesh_terms: D000818:Animals; D001141:Aromatase / Q000235:genetics* / Q000378:metabolism; D001921:Brain / Q000378:metabolism*; D001931:Brain Mapping; D049109:Cell Proliferation; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D005260:Female; D008297:Male; D009457:Neuroglia / Q000166:cytology / Q000378:metabolism; D015027:Zebrafish / Q000378:metabolism; D029961:Zebrafish Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D029961:Zebrafish Proteins; C490175:fabp7a protein, zebrafish; D001141:Aromatase; C440728:Cyp19a1b protein, zebrafish
keywords: Aromatase B; Blbp; Estrogen; Neurogenesis; Radial glial cells; Zebrafish