title: Apoptotic and antioxidant activities of methanol extract of Mussaenda roxburghii leaves.
abstract: roxburghii. Anticancer activity of MMR has been carried out on Ehrlich ascites carcinoma (EAC) cells with three different doses (20, 40 and 60 mg/kg/day) by observing different parameters such as tumor weight, survival time of EAC-bearing mice, growth inhibition of EAC cells, morphological changes and nuclear damage of EAC cells etc. whereas antioxidant activity was determined by measuring total antioxidant, DPPH free radical scavenging, ferrous reducing capacity assay. The extract showed highest anticancer activity at 60 mg/kg day¬-⁻¹(i.p.). It caused 81.4% (P<0.01) cells growth inhibition and reduced tumor burden significantly (78.5%; P<0.001) in comparison to control. It also increased life span of EAC-bearing mice significantly (73.5%; P<0.01). MMR treated EAC cells showed membrane blebbing, chromatin condensation, nuclear fragmentation (apoptotic feature) in Hoechst 33342 staining under fluorescence microscope. DNA fragmentation assay in agarose gel (1.5%) electrophoresis also rectified that it causes EAC cells death by apoptosis. MMR also exhibited moderate antioxidant properties in dose dependent manner. Thus, this plant can therefore be considering a resource for natural chemo-preventive drugs as well as a possible pharmaceutical supplement.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi, Bangladesh.
authors.forename: Farhadul
authors.initials: F
authors.lastname: Islam
authors.affiliation: Department of Pharmacy, Jessore Science and Technology University, Jessore, Bangladesh.
authors.forename: Obayed
authors.initials: O
authors.lastname: Raihan
authors.affiliation: Department of Pharmacy, International Islamic University Chittagong, Chittagong, Bangladesh.
authors.forename: Dipjoy
authors.initials: D
authors.lastname: Chowdhury
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi, Bangladesh.
authors.forename: Mahbuba
authors.initials: M
authors.lastname: Khatun
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi, Bangladesh.
authors.forename: Natasha
authors.initials: N
authors.lastname: Zuberi
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi, Bangladesh.
authors.forename: Laboni
authors.initials: L
authors.lastname: Khatun
authors.affiliation: Department of Pharmacy, University of Rajshahi, Rajshahi, Bangladesh.
authors.forename: Afrina
authors.initials: A
authors.lastname: Brishti
authors.affiliation: Department of Pharmacy, International Islamic University Chittagong, Chittagong, Bangladesh.
authors.forename: Entaz
authors.initials: E
authors.lastname: Bahar
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D017209:Apoptosis / Q000187:drug effects*; D001713:Biphenyl Compounds / Q000737:chemistry; D002286:Carcinoma, Ehrlich Tumor / Q000188:drug therapy* / Q000473:pathology; D045744:Cell Line, Tumor; D053938:DNA Fragmentation; D004305:Dose-Response Relationship, Drug; D007928:Lethal Dose 50; D008297:Male; D000432:Methanol / Q000737:chemistry*; D051379:Mice; D008517:Phytotherapy; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D018515:Plant Leaves; D010946:Plants, Medicinal; D019666:Rubiaceae / Q000737:chemistry; D012997:Solvents / Q000737:chemistry*; D013997:Time Factors; D047368:Tumor Burden
chemical_list: D000970:Antineoplastic Agents; D000975:Antioxidants; D001713:Biphenyl Compounds; D010851:Picrates; D010936:Plant Extracts; D012997:Solvents; C004931:1,1-diphenyl-2-picrylhydrazyl; D000432:Methanol