title: Effect of autologous platelet-rich plasma on the chondrogenic differentiation of rabbit adipose-derived stem cellsin vitro.
abstract: This study aimed to isolate rabbit adipose-derived stem cells (ADSCs) and explore the potential of platelet-rich plasma (PRP) in the chondrogenic differentiation of ADSCs, thereby potentially providing a new approach for the repair and regeneration of cartilage injury. Rabbit ADSCs were isolated and characterized by induction towards adipogenic, osteogenic and chondrogenic lineagesin vitro. The isolated ADSCs were also cultured with or without 10% PRP. Immunofluorescence staining, toluidine blue staining and reverse transcription-quantitative polymerase chain reaction (RT-qPCR) were used to detect type II collagen (Col II) and aggrecan (AGC) expression. Col II immunofluorescence staining and toluidine blue staining indicated that following induction by autologous PRP, ADSCs manifested Col II and AGC expression. The expression of Col II and AGC mRNA was significantly upregulated in the PRP-treated cells when compared with that in control cells. Autologous PRP produced by laboratory centrifugation was able to promote the chondrogenic differentiation of rabbit ADSCsin vitro.
authors.affiliation: Department of Orthopedics, Jianhu Hospital, Medical School of Nantong University, Jianhu, Jiangsu 224700, P.R. China.
authors.forename: Xiao-Bo
authors.initials: XB
authors.lastname: Tang
authors.affiliation: Department of Orthopedics, Jianhu Hospital, Medical School of Nantong University, Jianhu, Jiangsu 224700, P.R. China.
authors.forename: Pei-Long
authors.initials: PL
authors.lastname: Dong
authors.affiliation: Department of Orthopedics, Jianhu Hospital, Medical School of Nantong University, Jianhu, Jiangsu 224700, P.R. China.
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Orthopedics, Nanjing Integrative Medicine Hospital, Nanjing University of Traditional Chinese Medicine, Nanjing, Jiangsu 210014, P.R. China.
authors.forename: Hai-Yang
authors.initials: HY
authors.lastname: Zhou
authors.affiliation: Department of Orthopedics, Zhongda Hospital, Medical School of Southeast University, Nanjing, Jiangsu 210009, P.R. China.
authors.forename: Hai-Xiang
authors.initials: HX
authors.lastname: Zhang
authors.affiliation: Department of Orthopedics, Zhongda Hospital, Medical School of Southeast University, Nanjing, Jiangsu 210009, P.R. China.
authors.forename: Shan-Zheng
authors.initials: SZ
authors.lastname: Wang
keywords: adipose-derived stem cells; chondrogenic differentiation; growth factors; platelet-rich plasma