title: Cinidium officinale and its Bioactive Compound, Butylidenephthalide, Inhibit Laser-Induced Choroidal Neovascularization in a Rat Model.
abstract: Choroidal neovascularization (CNV) is a common pathology in age-related macular degeneration. In this study, we evaluated in a rat model the effect of an extract of Cinidium officinale Makino and its bioactive compound, butylidenephthalide, on laser-induced CNV. Experimental CNV was induced in Long-Evans rats by laser photocoagulation. C. officinale extract (COE) and butylidenephthalide was intraperitoneally injected once per day for ten days after laser photocoagulation. Choroidal flat mounts were prepared to measure CNV areas and macrophage infiltration. We used a protein array to evaluate the expression levels of angiogenic factors. The CNV area and macrophage infiltration in COE-treated rats were significantly lower than in vehicle-treated rats. COE decreased the expression levels of IGFBP-1, MCP-1, PAI-1, and VEGF. Additionally, butylidenephthalide also inhibited the laser-induced CNV formation and macrophage infiltration and down-regulated the expression of IGFBP-1, MCP-1 and VEGF. These results suggest that COE exerts anti-angiogenic effects on laser-induced CNV by inhibiting the expression of IGFBP-1, MCP-1, and VEGF, indicating that anti-angiogenic activities of COE may be in part due to its bioactive compound, butylidenephthalide.
authors.affiliation: College of Pharmacy, Chungnam National University, Daejeon 34134, Korea. candykong@kiom.re.kr.|Korean Medicine Convergence Research Division, Korea Institute of Oriental Medicine, Daejeon 34054, Korea. candykong@kiom.re.kr.
authors.forename: Yun Mi
authors.initials: YM
authors.lastname: Lee
authors.affiliation: Korean Medicine Convergence Research Division, Korea Institute of Oriental Medicine, Daejeon 34054, Korea. yrsanta@kiom.re.kr.
authors.forename: Yu Ri
authors.initials: YR
authors.lastname: Lee
authors.affiliation: Korean Medicine Convergence Research Division, Korea Institute of Oriental Medicine, Daejeon 34054, Korea. jskim@kiom.re.kr.
authors.forename: Jin Sook
authors.initials: JS
authors.lastname: Kim
authors.affiliation: College of Pharmacy, Chungnam National University, Daejeon 34134, Korea. yhk@cnu.ac.kr.
authors.forename: Young Ho
authors.initials: YH
authors.lastname: Kim
authors.affiliation: Korean Medicine Convergence Research Division, Korea Institute of Oriental Medicine, Daejeon 34054, Korea. dvmhyun@kiom.re.kr.
authors.forename: Junghyun
authors.initials: J
authors.lastname: Kim
mesh_terms: D020533:Angiogenesis Inhibitors / Q000737:chemistry / Q000494:pharmacology; D000818:Animals; D018932:Chemokine CCL2 / Q000235:genetics / Q000378:metabolism; D020256:Choroidal Neovascularization / Q000188:drug therapy / Q000209:etiology / Q000378:metabolism / Q000473:pathology*; D004195:Disease Models, Animal; D005786:Gene Expression Regulation / Q000187:drug effects; D018970:Insulin-Like Growth Factor Binding Protein 1 / Q000235:genetics / Q000378:metabolism; D007834:Lasers / Q000009:adverse effects; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D010796:Phthalic Anhydrides / Q000737:chemistry / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism
chemical_list: D020533:Angiogenesis Inhibitors; D018932:Chemokine CCL2; D018970:Insulin-Like Growth Factor Binding Protein 1; D010796:Phthalic Anhydrides; D010936:Plant Extracts; D042461:Vascular Endothelial Growth Factor A; C026105:butylidenephthalide
keywords: Cinidium officinale; age-related macular degeneration; butylidenephthalide; choroidal neovascularization