title: The first protocol of stable isotope ratio assessment in tumor tissues based on original research.
abstract: Thanks to proteomics and metabolomics, for the past several years there has been a real explosion of information on the biology of cancer, which has been achieved by spectroscopic methods, including mass spectrometry. These modern techniques can provide answers to key questions about tissue structure and mechanisms of its pathological changes. However, despite the thousands of spectroscopic studies in medicine, there is no consensus on issues ranging from the choice of research tools, acquisition and preparation of test material to the interpretation and validation of the results, which greatly reduces the possibility of transforming the achieved knowledge to progress in the treatment of individual patients. The aim of this study was to verify the utility of isotope ratio mass spectrometry in the evaluation of tumor tissues. Based on experimentation on animal tissues and human neoplasms, the first protocol of stable isotope ratio assessment of carbon and nitrogen isotopes in tumor tissues was established.
authors.affiliation: Katarzyna Taran MD, PhD, Department of Pathology, Medical University of Lodz, Pomorska 251, 92-216 Lodz, Poland, tel. +48 42 675 76 35, e-mail: dr.taran.patho@gmail.com.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Taran
authors.forename: Toma
authors.initials: T
authors.lastname: Frączek
authors.forename: Rafal
authors.initials: R
authors.lastname: Kamiński
authors.forename: Anna
authors.initials: A
authors.lastname: Sitkiewicz
authors.forename: Jozef
authors.initials: J
authors.lastname: Kobos
authors.forename: Piotr
authors.initials: P
authors.lastname: Paneth
mesh_terms: D000818:Animals; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007554:Isotopes / Q000032:analysis*; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D009369:Neoplasms / Q000737:chemistry*
chemical_list: D007554:Isotopes