title: Expression of Multiple Functional RNAs or Proteins from One Viral Vector.
abstract: In this chapter, we will cover the available design choices for enabling expression of two functional protein or RNA sequences from a single viral vector. Such vectors are very useful in the neuroscience-related field of neuronal control and modulation, e.g., using optogenetics or DREADDs, but are also desirable in applications of CRISPR/Cas9 in situ genome editing and more refined therapeutic approaches. Each approach to achieving this combined expression has its own strengths and limitations, which makes them more or less suitable for different applications. In this chapter, we describe the available alternatives and provide tips on how they can be implemented.
authors.affiliation: Molecular Neuromodulation, Wallenberg Neuroscience Center, Lund University, 117, 221 00, Lund, Sweden. tomas.bjorklund@med.lu.se.
authors.forename: Tomas
authors.initials: T
authors.lastname: Björklund
mesh_terms: D064113:CRISPR-Cas Systems; D002490:Central Nervous System / Q000378:metabolism; D000229:Dependovirus / Q000235:genetics; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics; D016086:Lentivirus / Q000235:genetics; D011506:Proteins / Q000235:genetics*; D012313:RNA / Q000235:genetics*; D019076:Transgenes
chemical_list: D011506:Proteins; D012313:RNA
keywords: Adeno-associated viral vector; Bicistronic; Bidirectional promoter; Cloning; Gene therapy; Genome editing; IRES; In vivo gene transfer; Lentiviral vector; Ribosome skipping; Transgene expression