title: Nano-TiO2 Is Not Phytotoxic As Revealed by the Oilseed Rape Growth and Photosynthetic Apparatus Ultra-Structural Response.
abstract: Recently nano-materials are widely used but they have shown contrasting effects on human and plant life. Keeping in view the contrasting results, the present study has evaluated plant growth response, antioxidant system activity and photosynthetic apparatus physiological and ultrastructural changes in Brassica napus L. plants grown under a wide range (0, 500, 2500, 4000 mg/l) of nano-TiO2 in a pot experiment. Nano-TiO2 has significantly improved the morphological and physiological indices of oilseed rape plants under our experimental conditions. All the parameters i-e morphological (root length, plant height, fresh biomass), physiological (photosynthetic gas exchange, chlorophyll content, nitrate reductase activity) and antioxidant system (Superoxide dismutase, SOD; Guaiacol peroxidase, POD; Catalase, CAT) recorded have shown improvement in their performance by following nano-TiO2 dose-dependent manner. No significant chloroplast ultra-structural changes were observed. Transmission electron microscopic images have shown that intact & typical grana and stroma thylakoid membranes were in the chloroplast, which suggest that nano-TiO2 has not induced the stressful environment within chloroplast. Finally, it is suggested that, nano-TiO2 have growth promoting effect on oilseed rape plants.
authors.affiliation: Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.affiliation: Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan, PR China.|Department of Agronomy, University of Agriculture, Faisalabad, Pakistan.
authors.forename: Muhammad Shahbaz
authors.initials: MS
authors.lastname: Naeem
authors.affiliation: College of Life Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao, PR China.
authors.forename: Xiuping
authors.initials: X
authors.lastname: Wang
authors.affiliation: Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan, PR China.
authors.forename: Lixin
authors.initials: L
authors.lastname: Liu
authors.affiliation: Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan, PR China.
authors.forename: Chang
authors.initials: C
authors.lastname: Chen
authors.affiliation: Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan, PR China.
authors.forename: Ni
authors.initials: N
authors.lastname: Ma
authors.affiliation: Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan, PR China.
authors.forename: Chunlei
authors.initials: C
authors.lastname: Zhang
mesh_terms: D000975:Antioxidants / Q000378:metabolism; D001673:Biodegradation, Environmental; D029688:Brassica napus / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D029741:Brassica rapa / Q000187:drug effects* / Q000254:growth & development* / Q000378:metabolism; D002104:Cadmium / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D002734:Chlorophyll / Q000378:metabolism; D002736:Chloroplasts / Q000187:drug effects / Q000378:metabolism; D018527:Hydroponics / Q000379:methods; D008315:Malondialdehyde / Q000378:metabolism; D049329:Nanostructures / Q000633:toxicity*; D009195:Peroxidase / Q000378:metabolism; D010788:Photosynthesis / Q000187:drug effects*; D018515:Plant Leaves / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D012989:Soil Pollutants / Q000633:toxicity; D013312:Stress, Physiological / Q000187:drug effects; D013482:Superoxide Dismutase / Q000378:metabolism; D014025:Titanium / Q000633:toxicity*
chemical_list: D000975:Antioxidants; D012989:Soil Pollutants; D002104:Cadmium; D002734:Chlorophyll; C009495:titanium dioxide; D008315:Malondialdehyde; D014025:Titanium; C121048:guaiacol peroxidase; D002374:Catalase; D009195:Peroxidase; D013482:Superoxide Dismutase