title: Metabolic engineering of Saccharomyces cerevisiae for linalool production.
abstract: OBJECTIVE
  To engineer the yeast Saccharomyces cerevisiae for the heterologous production of linalool.
  
  
  RESULTS
  Expression of linalool synthase gene from Lavandula angustifolia enabled heterologous production of linalool in S. cerevisiae. Downregulation of ERG9 gene, that encodes squalene synthase, by replacing its native promoter with the repressible MET3 promoter in the presence of methionine resulted in accumulation of 78 µg linalool l(-1) in the culture medium. This was more than twice that produced by the control strain. The highest linalool titer was obtained by combined repression of ERG9 and overexpression of tHMG1. The yeast strain harboring both modifications produced 95 μg linalool l(-1).
  
  
  CONCLUSIONS
  Although overexpression of tHMG1 and downregulation of ERG9 enhanced linalool titers threefold in the engineered yeast strain, alleviating linalool toxicity is necessary for further improvement of linalool biosynthesis in yeast.
authors.affiliation: Department of Agricultural Biotechnology, College of Agriculture, Isfahan University of Technology, Isfahan, 84156-83111, Iran.
authors.forename: Pegah
authors.initials: P
authors.lastname: Amiri
authors.affiliation: Department of Agricultural Biotechnology, College of Agriculture, Isfahan University of Technology, Isfahan, 84156-83111, Iran.
authors.forename: Azar
authors.initials: A
authors.lastname: Shahpiri
authors.affiliation: Department of Biotechnology, Faculty of Advanced Sciences and Technologies, University of Isfahan, Isfahan, 81746-73441, Iran. ma.asadollahi@ast.ui.ac.ir.
authors.forename: Mohammad Ali
authors.initials: MA
authors.lastname: Asadollahi
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemistry, University of Isfahan, Isfahan, 81746-73441, Iran.
authors.forename: Fariborz
authors.initials: F
authors.lastname: Momenbeik
authors.affiliation: Department of Chemical Engineering & Applied Chemistry, University of Toronto, 200 College Street, Toronto, ON, M5S 3E5, Canada.
authors.forename: Siavash
authors.initials: S
authors.lastname: Partow
mesh_terms: D000080462:Acyclic Monoterpenes; D015536:Down-Regulation; D013186:Farnesyl-Diphosphate Farnesyltransferase / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D006836:Hydro-Lyases / Q000235:genetics / Q000378:metabolism; D027523:Lavandula / Q000201:enzymology / Q000235:genetics; D060847:Metabolic Engineering; D053858:Metabolic Networks and Pathways / Q000235:genetics; D039821:Monoterpenes / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D000080462:Acyclic Monoterpenes; D039821:Monoterpenes; D011994:Recombinant Proteins; D029701:Saccharomyces cerevisiae Proteins; C018584:linalool; C496127:ERG9 protein, S cerevisiae; D013186:Farnesyl-Diphosphate Farnesyltransferase; D006836:Hydro-Lyases; C092055:S-linalool synthase
keywords: Lavandula angustifolia; Linalool; Linalool synthase; Metabolic engineering; Monoterpene; Saccharomyces cerevisiae