title: Role of an endothelin type A receptor antagonist in regulating torsion-induced testicular apoptosis in rats.
abstract: Testicular torsion is a well-known medical emergency that can lead to pathological changes in the testicular tissues and male infertility. This investigation was undertaken to gain insight into the effects of an endothelin type A receptor antagonist (BQ123) on torsion-induced germ cell loss. Twenty-eight male Wistar albino rats were divided into four groups. In group I (control group), a sham operation to the left testis was performed. In group II (I/R injury), I/R injury was created by rotating the left testis 720° in a clockwise direction for 2 h and detorsing the testis after 2 h. In group III (I/R injury+BQ123), the rats were subjected to I/R injury and BQ123 injection (1 mg/kg, intravenous). In group IV (control+BQ123), the sham operated rats were subjected to BQ123. The testes of the rats were removed in all groups. Torsion-induced apoptosis and the effects of BQ123 were examined by the terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate (dUTP) nick end labelling (TUNEL) technique, immunohistochemistry and western blotting. In group II, the number of TUNEL-positive cells increased after testicular torsion. Immunohistochemistry and western blotting showed that apoptotic proteins (active caspase 3 and Bax) were upregulated, and the anti-apoptotic protein Bcl2 was downregulated in I/R injury. The administration of BQ123 caused a significant decrease in the number of apoptotic cells and the expression of apoptotic proteins (p<0.05) when compared with the I/R injury group. No significant effect of BQ123 was observed in the testicular cells of group IV. This animal study provides evidence of the regulatory effects of BQ123 on torsion-induced testicular apoptosis.
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, Yildirim Beyazit University, Ankara, Turkey sevilcayli@yahoo.com.
authors.forename: Sevil
authors.initials: S
authors.lastname: Cayli
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, Gaziosmanpasa University, Tokat, Turkey.
authors.forename: Seda
authors.initials: S
authors.lastname: Ocakli
authors.affiliation: Department of Pediatric Surgery, Faculty of Medicine, Gaziosmanpasa University, Tokat, Turkey.
authors.forename: Ufuk
authors.initials: U
authors.lastname: Senel
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, Gaziosmanpasa University, Tokat, Turkey.
authors.forename: Zafer
authors.initials: Z
authors.lastname: Karaca
authors.affiliation: Department of Urology, Faculty of Medicine, Gaziosmanpasa University, Tokat, Turkey.
authors.forename: Fikret
authors.initials: F
authors.lastname: Erdemir
authors.affiliation: Department of Endocrinology and Metabolism, Diskapi Teaching and Research Hospital, Ankara and Department of Internal Medicine, School of Medicine (Kastamonu), Hacettepe University, Turkey.
authors.forename: Tuncay
authors.initials: T
authors.lastname: Delibasi
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D004195:Disease Models, Animal; D065130:Endothelin A Receptor Antagonists / Q000494:pharmacology*; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D008297:Male; D010456:Peptides, Cyclic / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000473:pathology; D013086:Spermatic Cord Torsion / Q000473:pathology*
chemical_list: D065130:Endothelin A Receptor Antagonists; D010456:Peptides, Cyclic; C072247:cyclo(Trp-Asp-Pro-Val-Leu)