title: Evaluation of different solvent mixtures in esterifiable lipids extraction from microalgae Botryococcus braunii for biodiesel production.
abstract: Non-polar and polar solvents as well as their mixtures were tested for the extraction of microalgae lipids and thus, to evaluate their effect on total and esterifiable lipids extraction yields with potential to be converted to biodiesel. The obtained results show an increase in lipids and esterifiable lipids extraction yields when non-polar and polar solvent mixtures were used. The higher esterifiable lipids extraction yield was 19.2%wt (based on dry biomass) using a chloroform-methanol mixture (75%v/v of methanol), corresponding to a 98.9%wt esterifiable lipids extraction. In addition, esterifiable lipids extraction yield of 18.9%wt (based on dry biomass) was obtained when a petroleum ether-methanol mixture (75%v/v of methanol) was used, corresponding to a 96.9%wt esterifiable lipids extraction.
authors.affiliation: Scientific and Technological Bioresources Nucleus, Universidad de La Frontera, Casilla 54-D, Temuco, Chile.
authors.forename: Pamela
authors.initials: P
authors.lastname: Hidalgo
authors.affiliation: Scientific and Technological Bioresources Nucleus, Universidad de La Frontera, Casilla 54-D, Temuco, Chile; Department of Chemical Engineering, Universidad de La Frontera, Casilla 54-D, Temuco, Chile.
authors.forename: Gustavo
authors.initials: G
authors.lastname: Ciudad
authors.affiliation: Scientific and Technological Bioresources Nucleus, Universidad de La Frontera, Casilla 54-D, Temuco, Chile; Department of Chemical Engineering, Universidad de La Frontera, Casilla 54-D, Temuco, Chile; Centre for Biotechnology & Bioengineering (CeBiB), Universidad de La Frontera, Casilla 54-D, Temuco, Chile. Electronic address: rodrigo.navia@ufrontera.cl.
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Navia
mesh_terms: D056804:Biofuels / Q000382:microbiology*; D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D000460:Chlorophyta / Q000187:drug effects / Q000378:metabolism*; D004951:Esterification / Q000187:drug effects; D000431:Ethanol / Q000737:chemistry; D005227:Fatty Acids / Q000378:metabolism; D008055:Lipids / Q000302:isolation & purification*; D000432:Methanol / Q000737:chemistry; D058086:Microalgae / Q000187:drug effects / Q000378:metabolism*; D012997:Solvents / Q000494:pharmacology*
chemical_list: D056804:Biofuels; D005227:Fatty Acids; D008055:Lipids; D012997:Solvents; D000431:Ethanol; D000432:Methanol
keywords: Lipid extraction; Microalgae; Non-polar solvents; Polar solvents