title: MicroRNA-323 regulates ischemia/reperfusion injury-induced neuronal cell death by targeting BRI3.
abstract: OBJECTIVE
  MicroRNA-323 (miR-323) has been reported to be upregulated in Ischemia/Reperfusion (I/R) injury-treated neuronal cell. However, the effect and underlying mechanism of miR-323 in I/R-induced neuronal cell death remains poorly understood. The current study was aim to investigate the role and molecular basis of miR-323 in I/R-induced neuronal cell.
  
  
  METHODS
  An oxygen-glucose deprivation (OGD) model of hippocampal neuron I/R was produced in vitro. Cell apoptosis, cell survival, and the expression of miR-323 were determined after 6 h, 12 h and 24 h after OGD treatment. The up- or down-regulation of miR-323 was performed by miR-323 mimics or anti-miR-323, respectively.
  
  
  RESULTS
  OGD induced apoptosis and suppressed survival in rat hippocampal neurons. And the expression levels of miR-323 were increased after OGD treatment. Furthermore, the up-regulation of miR-323 promoted apoptosis and suppressed survival, whereas the inhibition of miR-323 suppressed apoptosis and enhanced survival in OGD-treated neurons. Moreover, miR-323 could directly bind to BRI3 3'-UTR. Notably, the knockdown of BRI3 by BRI3 siRNA apparently abrogated cell survival and induced cell apoptosis in rat neurons.
  
  
  CONCLUSIONS
  This study indicated that miR-323 might regulate ischemia/reperfusion-induced rat neuronal cell death via targeting BRI3.
authors.affiliation: Department of Geratology, Pu Ai Hospital of Tongji Medical College, Huazhong University of Science and Technology Wuhan 430033, China.
authors.forename: Liu
authors.initials: L
authors.lastname: Yang
authors.affiliation: Department of Geratology, Pu Ai Hospital of Tongji Medical College, Huazhong University of Science and Technology Wuhan 430033, China.
authors.forename: Yin
authors.initials: Y
authors.lastname: Xiong
authors.affiliation: Department of Geratology, Pu Ai Hospital of Tongji Medical College, Huazhong University of Science and Technology Wuhan 430033, China.
authors.forename: Xian-Feng
authors.initials: XF
authors.lastname: Hu
authors.affiliation: Department of Geratology, Pu Ai Hospital of Tongji Medical College, Huazhong University of Science and Technology Wuhan 430033, China.
authors.forename: Yan-Hua
authors.initials: YH
authors.lastname: Du
mesh_terms: D020413:3' Untranslated Regions; D000818:Animals; D000831:Animals, Newborn; D017209:Apoptosis; D001665:Binding Sites; D016923:Cell Death; D015687:Cell Hypoxia; D002478:Cells, Cultured; D005786:Gene Expression Regulation; D005947:Glucose / Q000172:deficiency; D006624:Hippocampus / Q000378:metabolism* / Q000473:pathology; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009410:Nerve Degeneration; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism* / Q000473:pathology; D010100:Oxygen / Q000378:metabolism; D034622:RNA Interference; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000235:genetics / Q000378:metabolism* / Q000473:pathology / Q000517:prevention & control; D013997:Time Factors; D014162:Transfection
chemical_list: D020413:3' Untranslated Regions; C000610909:BRI3 protein, rat; C000610908:MIRN323 microRNA, rat; D008565:Membrane Proteins; D035683:MicroRNAs; D009419:Nerve Tissue Proteins; D005947:Glucose; D010100:Oxygen
keywords: BRI3; MicroRNA-323; ischemia/reperfusion injury; neuronal apoptosis