title: BmRobo2/3 is required for axon guidance in the silkworm Bombyx mori.
abstract: Axon guidance is critical for proper wiring of the nervous system. During the neural development, the axon guidance molecules play a key role and direct axons to choose the correct way to reach the target. Robo, as the receptor of axon guidance molecule Slit, is evolutionarily conserved from planarians to humans. However, the function of Robo in the silkworm, Bombyx mori, remained unknown. In this study, we cloned robo2/3 from B. mori (Bmrobo2/3), a homologue of robo2/3 in Tribolium castaneum. Moreover, BmRobo2/3 was localized in the neuropil, and RNAi-mediated knockdown of Bmrobo2/3 resulted in the longitudinal connectives forming closer to the midline. These data demonstrate that BmRobo2/3 is required for axon guidance in the silkworm.
authors.affiliation: Laboratory of Developmental Genetics, Shandong Agricultural University, Tai'an, Shandong 271018, China.
authors.forename: Xiao-Tong
authors.initials: XT
authors.lastname: Li
authors.affiliation: Laboratory of Developmental Genetics, Shandong Agricultural University, Tai'an, Shandong 271018, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Yu
authors.affiliation: Laboratory of Developmental Genetics, Shandong Agricultural University, Tai'an, Shandong 271018, China.
authors.forename: Qi-Sheng
authors.initials: QS
authors.lastname: Zhou
authors.affiliation: Laboratory of Developmental Genetics, Shandong Agricultural University, Tai'an, Shandong 271018, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Laboratory of Developmental Genetics, Shandong Agricultural University, Tai'an, Shandong 271018, China.
authors.forename: Zhao-Yang
authors.initials: ZY
authors.lastname: Liu
authors.affiliation: Laboratory of Developmental Genetics, Shandong Agricultural University, Tai'an, Shandong 271018, China. Electronic address: wzcui88@163.com.
authors.forename: Wei-Zheng
authors.initials: WZ
authors.lastname: Cui
authors.affiliation: Laboratory of Developmental Genetics, Shandong Agricultural University, Tai'an, Shandong 271018, China. Electronic address: liuqingxin@sdau.edu.cn.
authors.forename: Qing-Xin
authors.initials: QX
authors.lastname: Liu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001369:Axons / Q000378:metabolism* / Q000502:physiology; D001483:Base Sequence; D012831:Bombyx / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D017952:Ganglia, Invertebrate / Q000166:cytology / Q000378:metabolism; D019476:Insect Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D055495:Neurogenesis; D011971:Receptors, Immunologic / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
chemical_list: D019476:Insect Proteins; D011971:Receptors, Immunologic
keywords: Bombyx mori; Neural development; RNA interference; Robo; Slit