title: Production of Recombinant Proteins in the Chloroplast of the Green Alga Chlamydomonas reinhardtii.
abstract: Chloroplast transformation in the green algae Chlamydomonas reinhardtii can be used for the production of valuable recombinant proteins. Here, we describe chloroplast transformation of C. reinhardtii followed by protein detection. Genes of interest integrate stably by homologous recombination into the chloroplast genome following introduction by particle bombardment. Genes are inherited and expressed in lines recovered after selection in the presence of an antibiotic. Recombinant proteins can be detected by conventional techniques like immunoblotting and purified from liquid cultures.
authors.affiliation: Unidad Profesional Interdisciplinaria de Biotecnología, Instituto Politécnico Nacional, Av. Acueducto SN, Col. Barrio La Laguna Ticomán, CP. 07340, Mexico City, Mexico.
authors.forename: Daniel
authors.initials: D
authors.lastname: Guzmán-Zapata
authors.affiliation: Unidad Profesional Interdisciplinaria de Biotecnología, Instituto Politécnico Nacional, Av. Acueducto SN, Col. Barrio La Laguna Ticomán, CP. 07340, Mexico City, Mexico.
authors.forename: Karla Soledad
authors.initials: KS
authors.lastname: Macedo-Osorio
authors.affiliation: Unidad Profesional Interdisciplinaria de Biotecnología, Instituto Politécnico Nacional, Av. Acueducto SN, Col. Barrio La Laguna Ticomán, CP. 07340, Mexico City, Mexico.
authors.forename: Alma Lorena
authors.initials: AL
authors.lastname: Almaraz-Delgado
authors.affiliation: Unidad Profesional Interdisciplinaria de Biotecnología, Instituto Politécnico Nacional, Av. Acueducto SN, Col. Barrio La Laguna Ticomán, CP. 07340, Mexico City, Mexico.
authors.forename: Noé
authors.initials: N
authors.lastname: Durán-Figueroa
authors.affiliation: Unidad Profesional Interdisciplinaria de Biotecnología, Instituto Politécnico Nacional, Av. Acueducto SN, Col. Barrio La Laguna Ticomán, CP. 07340, Mexico City, Mexico. jbadilloc@ipn.mx.
authors.forename: Jesus Agustín
authors.initials: JA
authors.lastname: Badillo-Corona
mesh_terms: D019470:Biolistics; D016825:Chlamydomonas reinhardtii / Q000235:genetics* / Q000378:metabolism; D002736:Chloroplasts / Q000235:genetics* / Q000378:metabolism; D054628:Genome, Chloroplast; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics*; D014170:Transformation, Genetic
chemical_list: D011994:Recombinant Proteins
keywords: Chlamydomonas reinhardtii; Chloroplast biotechnology; Chloroplast transformation; Green algae; Particle bombardment; Recombinant protein