title: Metal induced self-assembly of designed V-shape protein into 2D wavy supramolecular nanostructure.
abstract: In order to understand and imitate the more complex bio-processes and fascinating functions in nature, protein self-assembly has been studied and has attracted more and more interest in recent years. Artificial self-assemblies of proteins have been constructed through many strategies. However, the design of complicated protein self-assemblies utilizing the special profile of building blocks remains a challenge. We herein report linear and 2D nanostructures constructed from a V shape SMAC protein and induced by metal coordination. Zigzag nanowires and wavy 2D nanostructures have been demonstrated by AFM and TEM. The zigzag nanowires can translate to a 2D nanostructure with an excess of metal ions, which reveals the step by step assembly process. Fluorescence and UV/Vis spectra have also been obtained to further study the mechanism and process of self-assembly. Upon the protein nanostructure, fluorescence resonance energy transfer (FRET) could also be detected using fluorescein modified proteins as building blocks. This article provides an approach for designing and controlling self-assembled protein nanostructures with a distinctive topological morphology.
authors.affiliation: State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun 130012, China. junqiuliu@jlu.edu.cn.
authors.forename: S P
authors.initials: SP
authors.lastname: Qiao
authors.affiliation: State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun 130012, China. junqiuliu@jlu.edu.cn.
authors.forename: C
authors.initials: C
authors.lastname: Lang
authors.affiliation: State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun 130012, China. junqiuliu@jlu.edu.cn.
authors.forename: R D
authors.initials: RD
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun 130012, China. junqiuliu@jlu.edu.cn.
authors.forename: X M
authors.initials: XM
authors.lastname: Li
authors.affiliation: State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun 130012, China. junqiuliu@jlu.edu.cn.
authors.forename: T F
authors.initials: TF
authors.lastname: Yan
authors.affiliation: State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun 130012, China. junqiuliu@jlu.edu.cn.
authors.forename: T Z
authors.initials: TZ
authors.lastname: Pan
authors.affiliation: State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun 130012, China. junqiuliu@jlu.edu.cn.
authors.forename: L L
authors.initials: LL
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun 130012, China. junqiuliu@jlu.edu.cn.
authors.forename: X T
authors.initials: XT
authors.lastname: Fan
authors.affiliation: State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun 130012, China. junqiuliu@jlu.edu.cn.
authors.forename: X
authors.initials: X
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun 130012, China. junqiuliu@jlu.edu.cn.
authors.forename: C X
authors.initials: CX
authors.lastname: Hou
authors.affiliation: State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun 130012, China. junqiuliu@jlu.edu.cn.
authors.forename: Q
authors.initials: Q
authors.lastname: Luo
authors.affiliation: State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun 130012, China. junqiuliu@jlu.edu.cn.
authors.forename: J Y
authors.initials: JY
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun 130012, China. junqiuliu@jlu.edu.cn.
authors.forename: J Q
authors.initials: JQ
authors.lastname: Liu