title: Stimulation of neuronal cells by culture supernatant of T lymphocytes triggered by anti-CD3 mAb followed by propagation in the presence of interleukin-2.
abstract: Performance status (PS) frequently improves occurs in cancer patients who have been infused with their own lymphokine-activated killer T cells (LAK-T). In the present study, a culture supernatant of LAK-T (LAK-T sup) administered to 8-week-old rats caused neurogenesis as evidenced by increased 5-ethynyl-2'-deoxyuridine staining of brain tissues. Intravenous injection of granulocyte-macrophage colony stimulating factor (GM-CSF), a major cytokine in LAK-T sup, had a similar effect. Furthermore, LAK-T sup induced Ca(++) increase in rat hippocampal brain slices that was detected in neuronal cells by emission of Fluo-8 NW at 520 nm. The same effect was observed with an rGM-CSF solution. GM-CSF may activate neuronal cells by stimulating the glial cells that surround and attach to them. If so, GM-CSF and LAK-T sup may improve the motor neurons of patients with amyotrophic lateral sclerosis. The neurogenerative effect of GM-CSF in LAK-T sup may also help improve brain function in aged adults including those with dementia such as Alzheimer's disease.
authors.affiliation: Choju Medical Institute, Fukushimura Hospital.|Department of Molecular Neurobiology, Graduate School of Medical Sciences, Nagoya City University, Japan.
authors.forename: Masae
authors.initials: M
authors.lastname: Ishiguro
authors.affiliation: Choju Medical Institute, Fukushimura Hospital.
authors.forename: Alan
authors.initials: A
authors.lastname: Okada
authors.affiliation: Department of Molecular Neurobiology, Graduate School of Medical Sciences, Nagoya City University, Japan.
authors.forename: Kiyofumi
authors.initials: K
authors.lastname: Asai
authors.affiliation: Choju Medical Institute, Fukushimura Hospital.
authors.forename: Kiyohide
authors.initials: K
authors.lastname: Kojima
authors.affiliation: Choju Medical Institute, Fukushimura Hospital.|Department of Molecular Neurobiology, Graduate School of Medical Sciences, Nagoya City University, Japan.
authors.forename: Hidechika
authors.initials: H
authors.lastname: Okada
mesh_terms: D000328:Adult; D000544:Alzheimer Disease / Q000628:therapy; D000690:Amyotrophic Lateral Sclerosis / Q000628:therapy; D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D001921:Brain / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D049109:Cell Proliferation / Q000502:physiology; D016207:Cytokines / Q000276:immunology; D005434:Flow Cytometry; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D006801:Humans; D016219:Immunotherapy, Adoptive / Q000379:methods; D007376:Interleukin-2 / Q000276:immunology / Q000494:pharmacology*; D015979:Killer Cells, Lymphokine-Activated / Q000166:cytology / Q000187:drug effects / Q000276:immunology* / Q000637:transplantation; D008297:Male; D055495:Neurogenesis / Q000276:immunology; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D013602:T-Lymphocytes, Cytotoxic / Q000187:drug effects* / Q000276:immunology; D015854:Up-Regulation
chemical_list: D000911:Antibodies, Monoclonal; D016207:Cytokines; D007376:Interleukin-2; D016178:Granulocyte-Macrophage Colony-Stimulating Factor
keywords: granulocyte-macrophage colony stimulating factor; lymphokine-activated killer T cells; neurogenesis; performance status