title: In vitro drug susceptibility of 40 international reference rapidly growing mycobacteria to 20 antimicrobial agents.
abstract: Rapidly growing mycobacteria (RGM) are human pathogens that are relatively easily identified by acid-fast staining but are proving difficult to treat in the clinic. In this study, we performed susceptibility testing of 40 international reference RGM species against 20 antimicrobial agents using the cation-adjusted Mueller-Hinton (CAMH) broth microdilution based on the minimum inhibitory concentration (MIC) assay recommended by the guidelines of the Clinical and Laboratory Standards Institute (CLSI). The results demonstrated that RGM organisms were resistant to the majority of first-line antituberculous agents but not to second-line fluoroquinolones or aminoglycosides. Three drugs (amikacin, tigecycline and linezolid) displayed potent antimycobacterial activity against all tested strains. Capreomycin, levofloxacin and moxifloxacin emerged as promising candidates for the treatment of RGM infections, and cefoxitin and meropenem were active against most strains. Mycobacterium chelonae (M. chelonae), M. abscessus, M. bolletii, M. fortuitum, M. boenickei, M. conceptionense, M. pseudoshottsii, M. septicum and M. setense were the most resistant RGM species. These results provide significant insight into the treatment of RGM species and will assist optimization of clinical criteria.
authors.affiliation: Department of Immunology, Changzhi Medical College Changzhi 046000, Shanxi, China ; State Key Laboratory of Infectious Diseases Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention & National Tuberculosis Reference Laboratory Beijing 102206, China ; Department of Immunology, Xiangya School of Medicine, Central South University Changsha 410078, Hunan, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Pang
authors.affiliation: State Key Laboratory of Infectious Diseases Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention & National Tuberculosis Reference Laboratory Beijing 102206, China ; Department of Physiology, Xiangya School of Medicine, Central South University Changsha 410078, Hunan, China.
authors.forename: Guilian
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Physiology, Xiangya School of Medicine, Central South University Changsha 410078, Hunan, China.
authors.forename: Li
authors.initials: L
authors.lastname: Wan
authors.affiliation: State Key Laboratory of Infectious Diseases Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention & National Tuberculosis Reference Laboratory Beijing 102206, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: State Key Laboratory of Infectious Diseases Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention & National Tuberculosis Reference Laboratory Beijing 102206, China.
authors.forename: Haican
authors.initials: H
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Infectious Diseases Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention & National Tuberculosis Reference Laboratory Beijing 102206, China.
authors.forename: Xiuqin
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Institute of Hepatology of Changzhi Medical College Changzhi 046000, Shanxi, China.
authors.forename: Zhongfu
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Infectious Diseases Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention & National Tuberculosis Reference Laboratory Beijing 102206, China ; Department of Physiology, Xiangya School of Medicine, Central South University Changsha 410078, Hunan, China.
authors.forename: Kanglin
authors.initials: K
authors.lastname: Wan
keywords: Drug susceptibility; antimicrobial; minimum inhibitory concentration (MIC); rapidly growing mycobacteria (RGM); reference strains