title: Cytotoxicity assays to evaluate tannery effluents treated by photoelectrooxidation.
abstract: The advanced oxidation process (AOP) is used to increase the treatment efficiency of effluents however, it is necessary to compare the toxicity of treated and untreated effluents to evaluate if the decontamination process does not cause any biological harm. Cultured cells have been previously used to assess the genotoxic and cytotoxic potential of various compounds. Hence, the aim of this work was to assess the applicability of cytotoxicity assays to evaluate the toxicity related to the AOP treatment. Samples of an industrial effluent were collected after their treatment by a conventional method. Cytotoxicity of standard and AOP treated effluents was assessed in CRIB and HEp-2 cell line using the MTT and neutral red assays. We observed decrease at cell viability in the both assays (50% MTT and 13% NRU) when cells were exposed to the AOP treatment in the highest concentration. Thus, cytotoxic assays in cultured cells can be explored as an useful method to evaluate toxicity as well as to optimize effluents treatment process.
authors.affiliation: Instituto de Ciências da Saúde, Universidade Feevale, Novo Hamburgo, RS, Brazil.
authors.forename: N
authors.initials: N
authors.lastname: Jaeger
authors.affiliation: Instituto de Ciências da Saúde, Universidade Feevale, Novo Hamburgo, RS, Brazil.
authors.forename: J P
authors.initials: JP
authors.lastname: Moraes
authors.affiliation: Programa de Pós-graduação em Qualidade Ambiental, Universidade Feevale, Novo Hamburgo, RS, Brazil.
authors.forename: C R
authors.initials: CR
authors.lastname: Klauck
authors.affiliation: Programa de Pós-graduação em Qualidade Ambiental, Universidade Feevale, Novo Hamburgo, RS, Brazil.
authors.forename: G
authors.initials: G
authors.lastname: Gehlen
authors.affiliation: Programa de Pós-graduação em Qualidade Ambiental, Universidade Feevale, Novo Hamburgo, RS, Brazil.
authors.forename: M A S
authors.initials: MA
authors.lastname: Rodrigues
authors.affiliation: Instituto de Ciências da Saúde, Universidade Feevale, Novo Hamburgo, RS, Brazil.
authors.forename: A L
authors.initials: AL
authors.lastname: Ziulkoski
mesh_terms: D000818:Animals; D002417:Cattle; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003603:Cytotoxins / Q000633:toxicity*; D055664:Electrochemical Techniques; D006801:Humans; D007220:Industrial Waste / Q000032:analysis; D010084:Oxidation-Reduction; D010782:Photolysis; D013633:Tanning; D018675:Toxicity Tests; D062065:Wastewater / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D003603:Cytotoxins; D007220:Industrial Waste; D062065:Wastewater; D014874:Water Pollutants, Chemical