title: Generation of Chimeras from Porcine Induced Pluripotent Stem Cells.
abstract: Pig induced pluripotent stem cells (piPSCs) offer a great opportunity and a number of advantages in the generation of transgenic animals. These immortalized cells can undergo multiple rounds of genetic modifications (e.g., gene knock-in, knockout) and selection leading to animals that have optimized traits of biomedical or agricultural interests. In this chapter we describe the production and characterization of piPSCs, microinjection of piPSCs into embryos, embryo transfer and production of chimeric animals based on successful protocols.
authors.affiliation: Department of Animal and Dairy Science, Regenerative Bioscience Center, University of Georgia, 425 River Road, RM 316, Athens, GA, 30602, USA. westfranklin1@gmail.com.
authors.forename: Franklin D
authors.initials: FD
authors.lastname: West
authors.affiliation: Minitube of America, Mt. Horeb, WI, USA.
authors.forename: Steve L
authors.initials: SL
authors.lastname: Terlouw
authors.affiliation: JRD Biotechnology, Oregon, WI, USA.
authors.forename: John R
authors.initials: JR
authors.lastname: Dobrinsky
authors.affiliation: Department of Animal and Dairy Science, Regenerative Bioscience Center, University of Georgia, 425 River Road, RM 316, Athens, GA, 30602, USA.|State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, Nanning, China.|Animal Reproduction Institute, Guangxi University, Nanning, China.
authors.forename: Yangqing
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Department of Animal and Dairy Science, Regenerative Bioscience Center, University of Georgia, 425 River Road, RM 316, Athens, GA, 30602, USA.
authors.forename: Erin T
authors.initials: ET
authors.lastname: Jordan
authors.affiliation: Department of Animal and Dairy Science, Regenerative Bioscience Center, University of Georgia, 425 River Road, RM 316, Athens, GA, 30602, USA.
authors.forename: Steven L
authors.initials: SL
authors.lastname: Stice
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D065150:Cellular Reprogramming; D004624:Embryo Transfer; D004622:Embryo, Mammalian / Q000166:cytology; D005260:Female; D005822:Genetic Vectors / Q000235:genetics; D007150:Immunohistochemistry; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism*; D008845:Microinjections; D011247:Pregnancy; D013552:Swine; D014157:Transcription Factors / Q000235:genetics; D014161:Transduction, Genetic
chemical_list: D014157:Transcription Factors
keywords: Embryo microinjection; Embryo transfer; Induced pluripotent stem cells; Pig