title: In-vitro cytotoxicity assessment of carbon-nanodot-conjugated Fe-aminoclay (CD-FeAC) and its bio-imaging applications.
abstract: We have investigated the cytotoxic assay of Fe-aminoclay (FeAC) nanoparticles (NPs) and simultaneous imaging in HeLa cells by photoluminescent carbon nanodots (CD) conjugation. Non-cytotoxic, photostable, and CD NPs are conjugated with cationic FeAC NPs where CD NPs play a role in bio-imaging and FeAC NPs act as a substrate for CD conjugation and help to uptake of NPs into cancer cells due to positively charged surface of FeAC NPs in physiological media. As increase of CD-FeAC NPs loading in HeLa cell in vitro, it showed slight cytotoxicity at 1000 μg/mL but no cytotoxicity for normal cells up to concentration of 1000 μg/mL confirmed by two 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and neutral red (NR) assays, with further observations by 4',6-diamidino-2-phenylindole (DAPI) stained confocal microscopy images, possessing that CD-FeAC NPs can be used as potential drug delivery platforms in cancer cells with simultaneous imaging. Graphical abstract CD conjugation with organo-building blocks of delaminated FeAC NPs.
authors.affiliation: Department of Chemical and Biomolecular Engineering (BK21+ Program), KAIST, 291 Daehakno, Yuseong-gu, Daejeon, 305-701, Republic of Korea. biormang@kaist.ac.kr.
authors.forename: Kyoung Suk
authors.initials: KS
authors.lastname: Kang
authors.affiliation: Advanced Nano-Surface Research Group, Korea Basic Science Institute (KBSI), Daejeon, 305-333, Republic of Korea. leeho@kbsi.re.kr.
authors.forename: Hyun Uk
authors.initials: HU
authors.lastname: Lee
authors.affiliation: Department of BioNano Technology, Gachon University, 1342 Seongnamdaero, Sujeong-gu, Seongnam-si, Gyeonggi-do, 461-701, Republic of Korea. moonil@gachon.ac.kr.
authors.forename: Moon Il
authors.initials: MI
authors.lastname: Kim
authors.affiliation: Advanced Nano-Surface Research Group, Korea Basic Science Institute (KBSI), Daejeon, 305-333, Republic of Korea. parkso86@naver.com.
authors.forename: So Young
authors.initials: SY
authors.lastname: Park
authors.affiliation: Department of Chemistry, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul, 156-756, Republic of Korea. sungjin.chang@gmail.com.
authors.forename: Sung-Jin
authors.initials: SJ
authors.lastname: Chang
authors.affiliation: Department of Bio and Brain Engineering (BK21+ Program), KAIST, 291 Daehakno, Yuseong-gu, Daejeon, 305-701, Republic of Korea. jihopark@kaist.ac.kr.
authors.forename: Ji-Ho
authors.initials: JH
authors.lastname: Park
authors.affiliation: Department of Biological Engineering, College of Engineering, Inha University, Incheon, 402-751, Republic of Korea. yunsuk.huh0311@gmail.com.
authors.forename: Yun Suk
authors.initials: YS
authors.lastname: Huh
authors.affiliation: Advanced Nano-Surface Research Group, Korea Basic Science Institute (KBSI), Daejeon, 305-333, Republic of Korea. jouhahn@kbsi.re.kr.
authors.forename: Jouhahn
authors.initials: J
authors.lastname: Lee
authors.affiliation: Division of Analytical Research, Korea Basic Science Institute (KBSI), Gangneung, 200-701, Republic of Korea. yangmino@kbsi.re.kr.
authors.forename: Mino
authors.initials: M
authors.lastname: Yang
authors.affiliation: Department of BioNano Technology, Gachon University, 1342 Seongnamdaero, Sujeong-gu, Seongnam-si, Gyeonggi-do, 461-701, Republic of Korea. dreamdbs@gachon.ac.kr.
authors.forename: Young-Chul
authors.initials: YC
authors.lastname: Lee
authors.affiliation: Department of Chemical and Biomolecular Engineering (BK21+ Program), KAIST, 291 Daehakno, Yuseong-gu, Daejeon, 305-701, Republic of Korea. hgpark1@kaist.ac.kr.
authors.forename: Hyun Gyu
authors.initials: HG
authors.lastname: Park
mesh_terms: D000818:Animals; D002244:Carbon / Q000737:chemistry*; D016923:Cell Death; D002470:Cell Survival; D004705:Endocytosis; D006367:HeLa Cells; D006801:Humans; D057446:Hydrodynamics; D021621:Imaging, Three-Dimensional / Q000379:methods*; D007211:Indoles / Q000378:metabolism; D007501:Iron / Q000737:chemistry*; D058085:Iron Compounds / Q000737:chemistry*; D051379:Mice; D018613:Microscopy, Confocal; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D017640:Silicates / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D017550:Spectroscopy, Fourier Transform Infrared; D055672:Static Electricity; D014961:X-Ray Diffraction
chemical_list: D007211:Indoles; D058085:Iron Compounds; D017640:Silicates; C007293:DAPI; D002244:Carbon; D007501:Iron