title: Facile Fabrication of a Silver Nanoparticle Immersed, Surface-Enhanced Raman Scattering Imposed Paper Platform through Successive Ionic Layer Absorption and Reaction for On-Site Bioassays.
abstract: We introduce a novel, facile, rapid, low-cost, highly reproducible, and power-free synthesizable fabrication method of paper-based silver nanoparticle (AgNP) immersed surface-enhanced Raman scattering (SERS) platform, known as the successive ionic layer absorption and reaction (SILAR) method. The rough and porous properties of the paper led to direct synthesis of AgNPs on the surface as well as in the paper due to capillary effects, resulting in improved plasmon coupling with interparticles and interlayers. The proposed SERS platform showed an enhancement factor of 1.1 × 10(9), high reproducibility (relative standard deviation of 4.2%), and 10(-12) M rhodamine B highly sensitive detection limit by optimizing the SILAR conditions including the concentration of the reactive solution (20/20 mM/mM AgNO3/NaBH4) and the number of SILAR cycles (six). The applicability of the SERS platform was evaluated using two samples including human cervical fluid for clinical diagnosis of human papillomavirus (HPV) infection, associated with cervical cancer, and a malachite green (MG) solution for fungicide and parasiticide in aquaculture, associated with human carcinogenesis. The AgNP-immersed SERS-functionalized platform using the SILAR technique allowed for high chemical structure sensitivity without additional tagging or chemical modification, making it a good alternative for early clinical diagnosis of HPV infection and detection of MG-activated human carcinogenesis.
authors.affiliation: Department of Medical Engineering, Graduate School, Kyung Hee University , Seoul, 02447, Korea.
authors.forename: Wansun
authors.initials: W
authors.lastname: Kim
authors.affiliation: Department of Obstetrics and Gynecology, The Catholic University of Korea , Kyonggi-do, 11765, Korea.
authors.forename: Yeon-Hee
authors.initials: YH
authors.lastname: Kim
authors.affiliation: Department of Medical Engineering, Graduate School, Kyung Hee University , Seoul, 02447, Korea.|Department of Biomedical Engineering, College of Medicine, Kyung Hee University , Seoul, 02447, Korea.
authors.forename: Hun-Kuk
authors.initials: HK
authors.lastname: Park
authors.affiliation: Department of Medical Engineering, Graduate School, Kyung Hee University , Seoul, 02447, Korea.|Department of Biomedical Engineering, College of Medicine, Kyung Hee University , Seoul, 02447, Korea.
authors.forename: Samjin
authors.initials: S
authors.lastname: Choi
mesh_terms: D001681:Biological Assay / Q000379:methods*; D015374:Biosensing Techniques / Q000379:methods*; D006801:Humans; D007477:Ions / Q000737:chemistry*; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry*; D010209:Paper; D030361:Papillomavirus Infections / Q000175:diagnosis; D016062:Porosity; D012834:Silver / Q000737:chemistry; D013059:Spectrum Analysis, Raman
chemical_list: D007477:Ions; D012834:Silver
keywords: AgNPs; HPV; SERS; SILAR; malachite green; on-site bioassay; paper substrate