title: Lewis Y antigen modified CD47 is an independent risk factor for poor prognosis and promotes early ovarian cancer metastasis.
abstract: CD47 is a membrane receptor that belongs to the immunoglobulin superfamily and plays an important role in the mechanisms of tumor immune escape. CD47 participates in tumor immune escape by combining with SIRPα to reduce the phagocytic activity of macrophages. There are six potential N-glycosylation sites on CD47, and glycosylation is known to be necessary for its membrane localization. However, it is still unknown to what extent glycosylation influences CD47 ligand binding properties and subsequent signaling. By using immunoprecipitation and confocal laser scanning microscopy, we showed that CD47 contains Lewis y antigen. Immunohistochemical analysis demonstrated that both the positive expression and the overexpression of CD47 and Lewis y antigen in cancer tissues and borderline tumors were significantly higher than those in benign ovarian tumors and normal ovarian tissues (P < 0.05). A linear correlation between the expression patterns of CD47 and Lewis y antigen was evident (r = 0.47, P < 0.01). The high expression of CD47 and Lewis y antigen showed significant correlations with the clinical pathological parameters of ovarian cancer [International Federation of Gynecology and Obstetrics (FIGO) standards, lymph node metastasis, and degree of differentiation] (P < 0.05). The Cox model and Kaplan-Meier tests showed that high expression of CD47 was an independent adverse risk factor for the prognosis of ovarian cancer. Cases with both high CD47 and Lewis y antigen expression had poor prognoses. Our study demonstrates that Lewis y antigens of CD47 may play a crucial role in the development of ovarian cancer, and could be new targets for immunotherapy for ovarian cancer.
authors.affiliation: Department of Gynecology and Obstetrics, Shengjing Hospital Affiliated to China Medical University Liaoning, China.
authors.forename: Mingzi
authors.initials: M
authors.lastname: Tan
authors.affiliation: Department of Gynecology and Obstetrics, Shengjing Hospital Affiliated to China Medical University Liaoning, China.
authors.forename: Liancheng
authors.initials: L
authors.lastname: Zhu
authors.affiliation: Department of Gynecology and Obstetrics, Shengjing Hospital Affiliated to China Medical University Liaoning, China ; Department of Gynecology and Obstetrics, Beijing Chaoyang Hospital Affiliated to Capital Medical University Beijing, China.
authors.forename: Huiyu
authors.initials: H
authors.lastname: Zhuang
authors.affiliation: Department of Gynecology and Obstetrics, Shengjing Hospital Affiliated to China Medical University Liaoning, China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Hao
authors.affiliation: Department of Gynecology and Obstetrics, Shengjing Hospital Affiliated to China Medical University Liaoning, China.
authors.forename: Song
authors.initials: S
authors.lastname: Gao
authors.affiliation: Department of Gynecology and Obstetrics, Shengjing Hospital Affiliated to China Medical University Liaoning, China.
authors.forename: Shuice
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Gynecology and Obstetrics, Shengjing Hospital Affiliated to China Medical University Liaoning, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Department of Gynecology and Obstetrics, Shengjing Hospital Affiliated to China Medical University Liaoning, China.
authors.forename: Dawo
authors.initials: D
authors.lastname: Liu
authors.affiliation: Department of Gynecology and Obstetrics, Shengjing Hospital Affiliated to China Medical University Liaoning, China.
authors.forename: Juanjuan
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Gynecology and Obstetrics, Shengjing Hospital Affiliated to China Medical University Liaoning, China.
authors.forename: Bei
authors.initials: B
authors.lastname: Lin
keywords: CD47; Lewis y; ovarian cancer; prognosis; tumor immune escape