title: Analysis of Chemical Constituents in Wuzi-Yanzong-Wan by UPLC-ESI-LTQ-Orbitrap-MS.
abstract: Wuzi-Yanzong-Wan (WZYZW), a classical traditional Chinese medicine (TCM) prescription containing Fructus Lych, Semen Cuscutae (fried), Fructus Rubi, Fructus Schisandrae chinensis (steamed) and Semen Plantaginis (fried with salt), is widely used to treat impotence, sterility, spermatorrhea, premature ejaculation, lumbago and post-micturation dribble. However, the chemical profile of WZYZW has not been established yet. In this work, a rapid and sensitive method for systematically screening and identifying the chemical constituents of WZYZW in both positive and negative ion modes using Ultra-Performance LC coupled with ESI-linear ion trap-Orbitrap tandem mass spectrometry (UPLC-ESI-LTQ-Orbitrap-MS) has been developed. Based on the chromatographic and spectrometric data, and referring to the literature, we could tentatively identify 106 compounds, including organic acids, flavonoids, phenylpropanoids, alkaloids and terpenoids. Fourteen ingredients from Fructus Lych were identified, while 10 ingredients were from Semen Cuscutae (fried), 33 ingredients were from Fructus Rubi, 37 ingredients were from Fructus Schisandrae chinensis (steamed), and 20 ingredients were from Semen Plantaginis (fried with salt). The results may provide essential data for further quality control, pharmacological research and clinical evaluation of WZYZW. Furthermore, this study indicates the developed approach based on UPLC-ESI-LTQ-Orbitrap-MS is suitable for characterizing the chemical profiles of TCM prescriptions. This is the first report to provide a comprehensive analysis of the chemical constituents of WZYZW.
authors.affiliation: Beijing Key Lab of Chinese Materia Medica Quality Evaluation, School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China. zoudixin@163.com.
authors.forename: Dixin
authors.initials: D
authors.lastname: Zou
authors.affiliation: Beijing Key Lab of Chinese Materia Medica Quality Evaluation, School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China. qing_wang_123@126.com.
authors.forename: Jinfeng
authors.initials: J
authors.lastname: Wang
authors.affiliation: Beijing Key Lab of Chinese Materia Medica Quality Evaluation, School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China. 13301080686@163.com.
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Beijing Tong Ren Tang Group Co., Ltd., Beijing 100051, China. 15301127363@163.com.
authors.forename: Suhua
authors.initials: S
authors.lastname: Xie
authors.affiliation: Beijing Key Lab of Chinese Materia Medica Quality Evaluation, School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China. xiaogangvsmylove@163.com.
authors.forename: Qing
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Beijing Key Lab of Chinese Materia Medica Quality Evaluation, School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China. 18547789137@163.com.
authors.forename: Kexin
authors.initials: K
authors.lastname: Xu
authors.affiliation: Beijing Key Lab of Chinese Materia Medica Quality Evaluation, School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China. linrch307@sina.com.
authors.forename: Ruichao
authors.initials: R
authors.lastname: Lin
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005419:Flavonoids / Q000032:analysis*; D008516:Medicine, Chinese Traditional; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D004365:Drugs, Chinese Herbal; D005419:Flavonoids; C528421:wuzi yanzong
keywords: UPLC-ESI-LTQ-Orbitrap-MS; Wuzi-Yanzong-Wan; constituents; identification