title: Competitive Immunoassays Using Antigen Microarrays.
abstract: In this work, a non-fouling antigen competitive immunoassay microarray based on the polymer brush is reported to detect multiple mycotoxins. The detection is achieved by utilizing highly specific monoclonal antibodies produced in our laboratory. The polymer brush, poly[oligo(ethylene glycol) methacrylate-co-glycidyl methacrylate] (POEGMA-co-GMA), is synthesized via surface-initiated atom transfer radical polymerization (SI-ATRP) on standard glass slides. In the polymer brush, the epoxy groups of glycidyl methacrylate (GMA) residues provide covalent binding sites for spotted antigens. Moreover, the abundant poly(ethylene glycol) (PEG) side chains in the brush are able to ultimately suppress the nonspecific protein adsorption in solution (non-fouling). The polymer brush shows a high and uniform protein loading, along with a high resistance to nonspecific protein absorption that are both important to achieve a highly sensitive immunoassay. As a demonstration of a multiplex assay, aflatoxin B1 (AFB1), ochratoxin A (OTA), and zearalenone (ZEN) are selected as antigen targets for simultaneous detections using the microarray.
authors.affiliation: Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan, 430062, People's Republic of China.
authors.forename: Zhaowei
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Institute for Clean Energy & Advanced Materials, Southwest University, Chongqing, 400715, People's Republic of China.
authors.forename: Weihua
authors.initials: W
authors.lastname: Hu
authors.affiliation: Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan, 430062, People's Republic of China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan, 430062, People's Republic of China. peiwuli@oilcrops.cn.
authors.forename: Peiwu
authors.initials: P
authors.lastname: Li
authors.affiliation: Institute for Clean Energy & Advanced Materials, Southwest University, Chongqing, 400715, People's Republic of China. ecmli@swu.edu.cn.
authors.forename: Changming
authors.initials: C
authors.lastname: Li
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000941:Antigens / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D004852:Epoxy Compounds / Q000737:chemistry; D005260:Female; D007114:Immunization; D007118:Immunoassay / Q000379:methods*; D008689:Methacrylates / Q000737:chemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D046228:Microarray Analysis / Q000379:methods*; D009183:Mycotoxins / Q000032:analysis / Q000737:chemistry / Q000276:immunology; D011092:Polyethylene Glycols / Q000737:chemistry
chemical_list: D000911:Antibodies, Monoclonal; D000941:Antigens; D004852:Epoxy Compounds; D008689:Methacrylates; D009183:Mycotoxins; C524499:polyethylene glycol methacrylate; D011092:Polyethylene Glycols; C007870:glycidyl methacrylate
keywords: Antigen microarray; Competitive immunoassay; Mycotoxins; Polymer brush