title: Use of the cobas 4800 system for the rapid detection of toxigenic Clostridium difficile and methicillin-resistant Staphylococcus aureus.
abstract: The new cobas® Cdiff and cobas® MRSA/SA tests were compared with conventional methods for the rapid detection of toxigenic Clostridium difficile and methicillin-resistant Staphylococcus aureus. The final concordance between cobas Cdiff Test and GDH/toxin gene screening was 97.62% and between cobas MRSA/SA Test and chromogenic culture, 91.30%, respectively.
authors.affiliation: Department of Microbiology, Complejo Hospitalario Universitario A Coruña-INIBIC, La Coruña, Spain.
authors.forename: Raquel
authors.initials: R
authors.lastname: Moure
authors.affiliation: Department of Microbiology, Complejo Hospitalario Universitario A Coruña-INIBIC, La Coruña, Spain.
authors.forename: Ángeles
authors.initials: Á
authors.lastname: Cañizares
authors.affiliation: Department of Microbiology, Complejo Hospitalario Universitario A Coruña-INIBIC, La Coruña, Spain.
authors.forename: María
authors.initials: M
authors.lastname: Muíño
authors.affiliation: Department of Microbiology, Complejo Hospitalario Universitario A Coruña-INIBIC, La Coruña, Spain.
authors.forename: Margarita
authors.initials: M
authors.lastname: Lobato
authors.affiliation: Department of Microbiology, Complejo Hospitalario Universitario A Coruña-INIBIC, La Coruña, Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Fernández
authors.affiliation: Department of Microbiology, Complejo Hospitalario Universitario A Coruña-INIBIC, La Coruña, Spain.
authors.forename: María
authors.initials: M
authors.lastname: Rodríguez
authors.affiliation: Department of Microbiology, Complejo Hospitalario Universitario A Coruña-INIBIC, La Coruña, Spain.
authors.forename: Maria José
authors.initials: MJ
authors.lastname: Gude
authors.affiliation: Department of Microbiology, Complejo Hospitalario Universitario A Coruña-INIBIC, La Coruña, Spain. Electronic address: MA.del.Mar.Tomas.Carmona@sergas.es.
authors.forename: Maria
authors.initials: M
authors.lastname: Tomás
authors.affiliation: Department of Microbiology, Complejo Hospitalario Universitario A Coruña-INIBIC, La Coruña, Spain. Electronic address: German.Bou.Arevalo@sergas.es.
authors.forename: Germán
authors.initials: G
authors.lastname: Bou
mesh_terms: D001427:Bacterial Toxins / Q000032:analysis* / Q000302:isolation & purification; D001431:Bacteriological Techniques / Q000295:instrumentation / Q000379:methods; D016360:Clostridioides difficile / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D003015:Clostridium Infections / Q000382:microbiology; D003470:Culture Media; D004768:Enterotoxins / Q000032:analysis / Q000302:isolation & purification; D006801:Humans; D055624:Methicillin-Resistant Staphylococcus aureus / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D025202:Molecular Diagnostic Techniques / Q000295:instrumentation* / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D013203:Staphylococcal Infections / Q000382:microbiology
chemical_list: D001427:Bacterial Toxins; D003470:Culture Media; D004768:Enterotoxins
keywords: Clostridium difficile; Cobas 4800; MRSA; Real-time PCR