title: Effect of BMAP-28 on human thyroid cancer TT cells is mediated by inducing apoptosis.
abstract: Thyroid cancer is the most common malignant endocrine tumor, with significant morbidity and mortality. Bovine myeloid antimicrobial peptide 28 (BMAP-28) is a cathelicidin that is found in bovine neutrophils. In the present study, the effect and relative mechanism of BMAP-28 on the human thyroid cancer TT cell linein vitroandin vivowere investigated. A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, flow cytometry and a TT-xenograft mouse model were used in this study. The data obtained indicated that BMAP-28 significantly inhibited the proliferation of the TT cellsin vitro. In addition, the Annexin V-fluorescein isothiocyanate/propidium iodide assay detected that BMAP-28 induced apoptotic effects in the TT cells. Moreover, the expression of activated caspase-3 and -9 was upregulated at the transcriptional and translational levels. Simultaneously, the expression of matrix metalloproteinase (MMP)3 and MMP9 was downregulated following BMAP-28 treatment. Finally, BMAP-28 significantly prevented the tumor growth in the TT-xenograft mouse model. These results indicated that BMAP-28 could be a potential agent for the treatment of thyroid cancer.
authors.affiliation: Division of Thyroid Surgery, Jilin Provincial Key Laboratory of Surgical Translational Medicine, China-Japan Union Hospital of Jilin Univeristy, Changchun, Jilin 130033, P.R. China.
authors.forename: Daqi
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Department of Anesthesiology, The Second Hospital of Jilin University, Jilin University School of Pharmaceutical Sciences, Changchun, Jilin 130033, P.R. China.
authors.forename: Lanlan
authors.initials: L
authors.lastname: Wan
authors.affiliation: Department of Neurosurgery, China-Japan Union Hospital of Jilin University, Changchun, Jilin 130033, P.R. China.
authors.forename: Jinnan
authors.initials: J
authors.lastname: Zhang
authors.affiliation: School of Life Science and Technology, China Pharmaceutical University, Nanjing, Jiangsu 210009, P.R. China.
authors.forename: Chang
authors.initials: C
authors.lastname: Liu
authors.affiliation: Division of Thyroid Surgery, Jilin Provincial Key Laboratory of Surgical Translational Medicine, China-Japan Union Hospital of Jilin Univeristy, Changchun, Jilin 130033, P.R. China.
authors.forename: Hui
authors.initials: H
authors.lastname: Sun
keywords: BMAP-28; TT cells; apoptosis; caspase-3; caspase-9; matrix metalloproteinase 3; matrix metalloproteinase 9; thyroid cancer