title: Facile Synthesis of Folic Acid-Modified Iron Oxide Nanoparticles for Targeted MR Imaging in Pulmonary Tumor Xenografts.
abstract: The purpose of this study was to develop folic acid (FA)-modified iron oxide (Fe3O4) nanoparticles (NPs) for targeted magnetic resonance imaging (MRI) of H460 lung carcinoma cells.
  
  
  
  Water-dispersible Fe3O4 NPs synthesized via a mild reduction method were conjugated with FA to generate FA-targeted Fe3O4 NPs. The specificity of FA-targeted Fe3O4 NPs to bind FA receptor was investigated in vitro by cellular uptake and cell MRI and in vivo by MRI of H460 tumors.
  
  
  
  The formed NPs displayed good biocompatibility and ultrahigh r 2 relaxivity (440.01/mM/s). The targeting effect of the NPs to H460 cells was confirmed by in vitro cellular uptake and cell MRI. H460 tumors showed a significant reduction in T2 signal intensity at 0.85 h, which then recovered and returned to control at 2.35 h.
  
  
  
  The results indicate that the prepared FA-targeted Fe3O4 NPs have potential to be used as T2 negative contrast agents in targeted MRI.
authors.affiliation: Department of Radiology, Shanghai General Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 200080, China.
authors.forename: Zaixian
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai, 201620, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Department of Radiology, Shanghai General Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 200080, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Radiology, Shanghai General Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 200080, China.
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Radiology, Shanghai General Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 200080, China.
authors.forename: Chengzhong
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Radiology, Shanghai General Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 200080, China.
authors.forename: Zhongling
authors.initials: Z
authors.lastname: Wang
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai, 201620, China. xshi@dhu.edu.cn.
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Shi
authors.affiliation: Department of Radiology, Shanghai General Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 200080, China. guixiangzhang@sina.com.
authors.forename: Guixiang
authors.initials: G
authors.lastname: Zhang
mesh_terms: D016923:Cell Death; D045744:Cell Line, Tumor; D004705:Endocytosis; D005290:Ferric Compounds / Q000737:chemistry*; D005492:Folic Acid / Q000737:chemistry*; D006801:Humans; D008175:Lung Neoplasms / Q000175:diagnosis*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D014018:Tissue Distribution; D023041:Xenograft Model Antitumor Assays
chemical_list: D005290:Ferric Compounds; C000499:ferric oxide; D005492:Folic Acid
keywords: FA; H460 cells; Iron oxide nanoparticles; MRI