title: A Novel Method of Somatic Cell Nuclear Transfer with Minimum Equipment.
abstract: Somatic cell nuclear transfer (SCNT) is an exceptional experimental biology technique with an arguably great contribution to our current understanding of developmental plasticity. Many students and young researchers are interested in taking advantage of SCNT virtues in their experiments but the cost of micromanipulation microscopes, intensive training programs, and also the sophisticated process of SCNT may dissuade them from entering this amazing field of science. Here, we describe the details of a streamlined manual method of SCNT that can be performed using very basic equipment found in every embryology laboratory: the Pasteur pipette and stereomicroscope. The overall method introduced is very simple and a person with no previous experience in cloning can learn and adopt the basic routines of this technique independently.
authors.affiliation: Department of Reproductive Biotechnology at Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, 16635-148, Isfahan, Iran. smhosseini@royaninstitute.org.
authors.forename: S M
authors.initials: SM
authors.lastname: Hosseini
authors.affiliation: Department of Reproductive Biotechnology at Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, 16635-148, Isfahan, Iran.
authors.forename: F
authors.initials: F
authors.lastname: Moulavi
authors.affiliation: Department of Reproductive Biotechnology at Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, 16635-148, Isfahan, Iran. mh.nasr-esfahani@royaninstitute.org.
authors.forename: M H
authors.initials: MH
authors.lastname: Nasr-Esfahani
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002453:Cell Cycle; D002459:Cell Fusion / Q000379:methods; D019976:Cloning, Organism / Q000295:instrumentation / Q000379:methods; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D008845:Microinjections / Q000379:methods; D053652:Nuclear Transfer Techniques / Q000295:instrumentation; D009865:Oocytes / Q000166:cytology; D012756:Sheep
keywords: Manual method; Pasteur pipette; Somatic cell nuclear transfer