title: Schizosaccharomyces pombe Homologs of Human DJ-1 Are Stationary Phase-Associated Proteins That Are Involved in Autophagy and Oxidative Stress Resistance.
abstract: The Parkinson's disease protein DJ-1 is involved in various cellular functions including detoxification of dicarbonyl compounds, autophagy and oxidative stress response. DJ-1 homologs are widely found in both prokaryotes and eukaryotes, constituting a superfamily of proteins that appear to be involved in stress response. Schizosaccharomyces pombe contains six DJ-1 homologs, designated Hsp3101-Hsp3105 and Sdj1 (previously named SpDJ-1). Here we show that deletion of any one of these six genes somehow affects autophagy during prolonged stationary phase. Furthermore, deletions of each of these DJ-1 homologs result in reduced stationary phase survival. Deletion of sdj1 also increases the sensitivity of stationary-phase cells to oxidative stress induced by hydrogen peroxide (H2O2) whereas overexpression of sdj1 has the opposite effect. Consistent with their role in stationary phase, expression of hsp3101, hsp3102, hsp3105 and sdj1, and to a lesser extent hsp3103 and hsp3104, is increased in stationary phase. The induction of hsp3101, hsp3102, hsp3105 and sdj1 involves the Sty1-regulated transcription factor Atf1 but not the transcription factor Pap1. Our results firmly establish that S. pombe homologs of DJ-1 are stationary-phase associated proteins and are likely involved in autophagy and antioxidant defense in stationary phase of S. pombe cells.
authors.affiliation: Jiangsu Key Laboratory for Microbes and Functional Genetics, College of Life Science, Nanjing Normal University, Nanjing, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Su
authors.affiliation: Jiangsu Key Laboratory for Microbes and Functional Genetics, College of Life Science, Nanjing Normal University, Nanjing, China.
authors.forename: Caiping
authors.initials: C
authors.lastname: Chen
authors.affiliation: Jiangsu Key Laboratory for Microbes and Functional Genetics, College of Life Science, Nanjing Normal University, Nanjing, China.
authors.forename: Linting
authors.initials: L
authors.lastname: Huang
authors.affiliation: Jiangsu Key Laboratory for Microbes and Functional Genetics, College of Life Science, Nanjing Normal University, Nanjing, China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Yan
authors.affiliation: Jiangsu Key Laboratory for Microbes and Functional Genetics, College of Life Science, Nanjing Normal University, Nanjing, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Huang
mesh_terms: D051697:Activating Transcription Factor 1 / Q000235:genetics; D000975:Antioxidants / Q000378:metabolism; D001343:Autophagy / Q000187:drug effects / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects / Q000235:genetics; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics*; D015513:Oncogene Proteins / Q000235:genetics*; D018384:Oxidative Stress / Q000187:drug effects / Q000235:genetics*; D000073718:Pancreatitis-Associated Proteins; D000071617:Protein Deglycase DJ-1; D012568:Schizosaccharomyces / Q000235:genetics*; D029702:Schizosaccharomyces pombe Proteins / Q000187:drug effects / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
chemical_list: D051697:Activating Transcription Factor 1; D000975:Antioxidants; D004268:DNA-Binding Proteins; D047908:Intracellular Signaling Peptides and Proteins; D015513:Oncogene Proteins; D000073718:Pancreatitis-Associated Proteins; C000612141:REG3A protein, human; D029702:Schizosaccharomyces pombe Proteins; D006861:Hydrogen Peroxide; C105131:PARK7 protein, human; D000071617:Protein Deglycase DJ-1