title: Stage-Specific Fatty Acid Fluxes Play a Regulatory Role in Glycerolipid Metabolism during Seed Development in Jatropha curcas L.
abstract: The present study describes the changes in lipid profile as well as fatty acid fluxes during seed development in Jatropha curcas L. Endosperm from 34, 37, and 40 days after anthesis (DAA), incubated with [(14)C]acetate, showed significant synthesis of phosphatidylcholine (PC) at seed maturation. The fatty acid methyl ester profile showed PC from 34 DAA was rich in palmitic acid (16:0), whereas PC from 37 and 40 DAA was rich in oleic acid (18:1n-9). Molecular species analysis of diacylglycerol (DAG) indicated DAG (16:0/18:2n-6) was in abundance at 34 DAA, whereas DAG (18:1n-9/18:2n-6) was significantly high at 40 DAA. Triacylglycerol (TAG) analysis revealed TAG (16:0/18:2n-6/16:0) was abundant at 34 DAA, whereas TAG (18:1n-9/18:2n-6/18:1n-9) formed the majority at 40 DAA. Expression of two types of diacylglycerol acyltransferases varied with seed maturation. These data demonstrate stage-specific distinct pools of PC and DAG synthesis during storage TAG accumulation in Jatropha seed.
authors.affiliation: Department of Plant Sciences, School of Life Sciences, University of Hyderabad , Hyderabad 500046, India.
authors.forename: Bharatula Sri Krishna
authors.initials: BS
authors.lastname: Chaitanya
authors.affiliation: Department of Plant Sciences, School of Life Sciences, University of Hyderabad , Hyderabad 500046, India.
authors.forename: Sumit
authors.initials: S
authors.lastname: Kumar
authors.affiliation: Council of Scientific and Industrial Research, Indian Institute of Chemical Technology , Hyderabad 500007, India.
authors.forename: Shiva Shanker
authors.initials: SS
authors.lastname: Kaki
authors.affiliation: Council of Scientific and Industrial Research, Indian Institute of Chemical Technology , Hyderabad 500007, India.
authors.forename: Marrapu
authors.initials: M
authors.lastname: Balakrishna
authors.affiliation: Council of Scientific and Industrial Research, Indian Institute of Chemical Technology , Hyderabad 500007, India.
authors.forename: Mallampalli Sri Lakshmi
authors.initials: MS
authors.lastname: Karuna
authors.affiliation: Council of Scientific and Industrial Research, Indian Institute of Chemical Technology , Hyderabad 500007, India.
authors.forename: Rachapudi Badari Narayana
authors.initials: RB
authors.lastname: Prasad
authors.affiliation: Department of Plant Sciences, School of Life Sciences, University of Hyderabad , Hyderabad 500046, India.
authors.forename: Pidaparty Seshadri
authors.initials: PS
authors.lastname: Sastry
authors.affiliation: Department of Plant Sciences, School of Life Sciences, University of Hyderabad , Hyderabad 500046, India.
authors.forename: Attipalli Ramachandra
authors.initials: AR
authors.lastname: Reddy
mesh_terms: D004075:Diglycerides / Q000032:analysis; D056625:Endosperm / Q000737:chemistry; D005227:Fatty Acids / Q000032:analysis*; D015870:Gene Expression; D020404:Glycerophospholipids / Q000378:metabolism*; D031286:Jatropha / Q000254:growth & development*; D019301:Oleic Acid / Q000032:analysis; D019308:Palmitic Acid / Q000032:analysis; D010713:Phosphatidylcholines / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism; D010743:Phospholipids / Q000032:analysis; D012639:Seeds / Q000737:chemistry* / Q000235:genetics / Q000254:growth & development*; D013997:Time Factors; D014280:Triglycerides / Q000032:analysis
chemical_list: D004075:Diglycerides; D005227:Fatty Acids; D020404:Glycerophospholipids; D010713:Phosphatidylcholines; D010743:Phospholipids; D014280:Triglycerides; D019301:Oleic Acid; D019308:Palmitic Acid
keywords: 18:1-rich TAG; Jatropha curcas; acyl-edited DAG; phosphatidylcholine