title: Genome-wide comparative chromosome maps of Arvicola amphibius, Dicrostonyx torquatus, and Myodes rutilus.
abstract: The subfamily Arvicolinae consists of a great number of species with highly diversified karyotypes. In spite of the wide use of arvicolines in biological and medicine studies, the data on their karyotype structures are limited. Here, we made a set of painting probes from flow-sorted chromosomes of a male Palearctic collared lemming (Dicrostonyx torquatus, DTO). Together with the sets of painting probes made previously from the field vole (Microtus agrestis, MAG) and golden hamster (Mesocricetus auratus, MAU), we carried out a reciprocal chromosome painting between these three species. The three sets of probes were further hybridized onto the chromosomes of the Eurasian water vole (Arvicola amphibius) and northern red-backed vole (Myodes rutilus). We defined the diploid chromosome number in D. torquatus karyotype as 2n = 45 + Bs and showed that the system of sex chromosomes is X1X2Y1. The probes developed here provide a genomic tool-kit, which will help to investigate the evolutionary biology of the Arvicolinae rodents. Our results show that the syntenic association MAG1/17 is present not only in Arvicolinae but also in some species of Cricetinae; and thus, should not be considered as a cytogenetic signature for Arvicolinae. Although cytogenetic signature markers for the genera have not yet been found, our data provides insight into the likely ancestral karyotype of Arvicolinae. We conclude that the karyotypes of modern voles could have evolved from a common ancestral arvicoline karyotype (AAK) with 2n = 56 mainly by centric fusions and fissions.
authors.affiliation: Institute of Molecular and Cellular Biology, SB RAS, Novosibirsk, 630090, Russia. rosa@mcb.nsc.ru.|Novosibirsk State University, Novosibirsk, 630090, Russia. rosa@mcb.nsc.ru.
authors.forename: Svetlana A
authors.initials: SA
authors.lastname: Romanenko
authors.affiliation: Institute of Molecular and Cellular Biology, SB RAS, Novosibirsk, 630090, Russia.
authors.forename: Natalya A
authors.initials: NA
authors.lastname: Lemskaya
authors.affiliation: Institute of Molecular and Cellular Biology, SB RAS, Novosibirsk, 630090, Russia.|Novosibirsk State University, Novosibirsk, 630090, Russia.
authors.forename: Vladimir A
authors.initials: VA
authors.lastname: Trifonov
authors.affiliation: Institute of Molecular and Cellular Biology, SB RAS, Novosibirsk, 630090, Russia.
authors.forename: Natalya A
authors.initials: NA
authors.lastname: Serdyukova
authors.affiliation: Department of Veterinary Medicine, Cambridge Resource Centre for Comparative Genomics, University of Cambridge, Madingley Road, Cambridge, CB3 OES, UK.
authors.forename: Patricia C M
authors.initials: PC
authors.lastname: O'Brien
authors.affiliation: A. N. Severtsov Institute of Ecology and Evolution, Moscow, 119071, Russia.
authors.forename: Nina Sh
authors.initials: NSh
authors.lastname: Bulatova
authors.affiliation: Zoological Institute, RAS, Saint-Petersburg, 190000, Russia.
authors.forename: Feodor N
authors.initials: FN
authors.lastname: Golenishchev
authors.affiliation: Department of Veterinary Medicine, Cambridge Resource Centre for Comparative Genomics, University of Cambridge, Madingley Road, Cambridge, CB3 OES, UK.
authors.forename: Malcolm A
authors.initials: MA
authors.lastname: Ferguson-Smith
authors.affiliation: Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SA, UK.
authors.forename: Fengtang
authors.initials: F
authors.lastname: Yang
authors.affiliation: Institute of Molecular and Cellular Biology, SB RAS, Novosibirsk, 630090, Russia.
authors.forename: Alexander S
authors.initials: AS
authors.lastname: Graphodatsky
mesh_terms: D000818:Animals; D003411:Arvicolinae / Q000235:genetics*; D005075:Biological Evolution; D002460:Cell Line; D002869:Chromosome Aberrations; D002871:Chromosome Banding; D002874:Chromosome Mapping / Q000379:methods*; D020223:Chromosome Painting / Q000379:methods*; D006224:Cricetinae; D005819:Genetic Markers / Q000235:genetics; D059785:Karyotype; D008647:Mesocricetus / Q000235:genetics*; D010802:Phylogeny; D012730:Sex Chromosomes / Q000235:genetics; D026801:Synteny / Q000235:genetics*
chemical_list: D005819:Genetic Markers
keywords: Comparative cytogenetics; Karyotype evolution; Lemming; Voles