title: Increased Expression of Neuregulin 1 and erbB2 Tyrosine Kinase in the Bladder of Rats With Cyclophosphamide-Induced Interstitial Cystitis.
abstract: OBJECTIVE
  The aim of this study was to evaluate changes in expressions of neuregulin (NRG)1 and erbB2 tyrosine kinase (ErbB2) in bladders of rats with cyclophosphamide (CYP)-induced interstitial cystitis (IC).
  
  
  METHODS
  Twenty-four Sprague-Dawley rats were divided into the IC group (n=16) and the control group (n=8). After inducing IC with intraperitoneal CYP injection, expressions of NRG1 and ErbB2 were analyzed using western blotting and reverse transcriptase-polymerase chain reaction.
  
  
  RESULTS
  In Western blotting, relative intensities and distributions of both NRG1 and ErbB2 were approximately 1.5- and 3.2-fold higher, respectively, in the IC group than in the control group (mean±standard deviation: 1.42±0.09 vs. 0.93±0.15 and 0.93±0.16 vs. 0.29±0.08, P<0.05). In the rat bladder samples, mRNA expression levels of NRG1 and ErbB2 were higher in the IC group than in the control group (P<0.05).
  
  
  CONCLUSIONS
  Our study has demonstrated significant changes in mRNA expression and immunoreactivity of NRG1 and ErbB2 receptors in the urinary bladder after CYP-induced IC. These results suggest that the up-regulated NRG1 may play a role in inducing an overactive bladder and promoting regeneration in the inflammatory bladder with CYP-induced IC.
authors.affiliation: Department of Urology, Chungnam National University School of Medicine, Daejeon, Korea.
authors.forename: Ki Hak
authors.initials: KH
authors.lastname: Song
authors.affiliation: Department of Urology, The Catholic University of Korea, Daejeon St. Mary's Hospital, Daejeon, Korea.
authors.forename: Chang Shik
authors.initials: CS
authors.lastname: Youn
authors.affiliation: Department of Urology, Chungnam National University School of Medicine, Daejeon, Korea.
authors.forename: Chung Lyul
authors.initials: CL
authors.lastname: Lee
authors.affiliation: Department of Urology, Chungnam National University School of Medicine, Daejeon, Korea.
authors.forename: Seung Woo
authors.initials: SW
authors.lastname: Yang
authors.affiliation: Department of Urology, Konyang University College of Medicine, Daejeon, Korea.
authors.forename: Young Seop
authors.initials: YS
authors.lastname: Chang
authors.affiliation: Department of Physiology, Yonsei University Wonju College of Medicine, Wonju, Korea.
authors.forename: Seoung Woo
authors.initials: SW
authors.lastname: Jeong
authors.affiliation: Department of Urology, Chungnam National University School of Medicine, Daejeon, Korea.
authors.forename: Chong Koo
authors.initials: CK
authors.lastname: Sul
keywords: Cystitis, Interstitial; Neuregulin-1; Rats; Receptor, ErbB-2