title: Glycosylation of Dentin Matrix Protein 1 is critical for osteogenesis.
abstract: Proteoglycans play important roles in regulating osteogenesis. Dentin matrix protein 1 (DMP1) is a highly expressed bone extracellular matrix protein that regulates both bone development and phosphate metabolism. After glycosylation, an N-terminal fragment of DMP1 protein was identified as a new proteoglycan (DMP1-PG) in bone matrix. In vitro investigations showed that Ser(89) is the key glycosylation site in mouse DMP1. However, the specific role of DMP1 glycosylation is still not understood. In this study, a mutant DMP1 mouse model was developed in which the glycosylation site S(89) was substituted with G(89) (S89G-DMP1). The glycosylation level of DMP1 was down-regulated in the bone matrix of S89G-DMP1 mice. Compared with wild type mice, the long bones of S89G-DMP1 mice showed developmental changes, including the speed of bone remodeling and mineralization, the morphology and activities of osteocytes, and activities of both osteoblasts and osteoclasts. These findings indicate that glycosylation of DMP1 is a key posttranslational modification process during development and that DMP1-PG functions as an indispensable proteoglycan in osteogenesis.
authors.affiliation: Tongji University, School of Stomatology, Laboratory of oral biomedical science and translational medicine, Shanghai, China, 200072.|Harbin Medical University, Institute of Hard Tissue Development and Regeneration, Harbin, China, 150086.
authors.forename: Yao
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Tongji University, School of Stomatology, Laboratory of oral biomedical science and translational medicine, Shanghai, China, 200072.
authors.forename: Yuteng
authors.initials: Y
authors.lastname: Weng
authors.affiliation: Tongji University, School of Stomatology, Laboratory of oral biomedical science and translational medicine, Shanghai, China, 200072.
authors.forename: Chenyang
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Tongji University, School of Stomatology, Laboratory of oral biomedical science and translational medicine, Shanghai, China, 200072.
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Harbin Medical University, Institute of Hard Tissue Development and Regeneration, Harbin, China, 150086.
authors.forename: Chen
authors.initials: C
authors.lastname: Kang
authors.affiliation: Tongji University, School of Stomatology, Laboratory of oral biomedical science and translational medicine, Shanghai, China, 200072.
authors.forename: Zhongshuang
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Tongji University, School of medicine, stem cell center, Shanghai, China, 200072.
authors.forename: Bo
authors.initials: B
authors.lastname: Jing
authors.affiliation: Tongji University, School of Stomatology, Laboratory of oral biomedical science and translational medicine, Shanghai, China, 200072.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Tongji University, School of Stomatology, Laboratory of oral biomedical science and translational medicine, Shanghai, China, 200072.
authors.forename: Zuolin
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000818:Animals; D001857:Bone Matrix / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D006031:Glycosylation; D051379:Mice; D010006:Osteoblasts / Q000378:metabolism; D010012:Osteogenesis / Q000235:genetics*
chemical_list: C513308:Dmp1 protein, mouse; D016326:Extracellular Matrix Proteins