title: Mass spectrometry based translational proteomics for biomarker discovery and application in colorectal cancer.
abstract: Colorectal cancer (CRC) is a leading cause of cancer-related death in the world. Clinically, early detection of the disease is the most effective approach to tackle this tough challenge. Discovery and development of reliable and effective diagnostic tools for the assessment of prognosis and prediction of response to drug therapy are urgently needed for personalized therapies and better treatment outcomes. Among many ongoing efforts in search for potential CRC biomarkers, MS-based translational proteomics provides a unique opportunity for the discovery and application of protein biomarkers toward better CRC early detection and treatment. This review updates most recent studies that use preclinical models and clinical materials for the identification of CRC-related protein markers. Some new advances in the development of CRC protein markers such as CRC stem cell related protein markers, SRM/MRM-MS and MS cytometry approaches are also discussed in order to address future directions and challenges from bench translational research to bedside clinical application of CRC biomarkers.
authors.affiliation: Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Sino-Africa Joint Research Center, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, P. R. China.|Haematology and Oncology Division, Children's Hospital Los Angeles, Los Angeles, CA, USA.
authors.forename: Hong
authors.initials: H
authors.lastname: Ma
authors.affiliation: Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Sino-Africa Joint Research Center, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, P. R. China.|University of Chinese Academy of Sciences, Beijing, P. R. China.
authors.forename: Guilin
authors.initials: G
authors.lastname: Chen
authors.affiliation: Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Sino-Africa Joint Research Center, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, P. R. China.
authors.forename: Mingquan
authors.initials: M
authors.lastname: Guo
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000235:genetics* / Q000378:metabolism; D003113:Colonoscopy; D015179:Colorectal Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology / Q000628:therapy; D004195:Disease Models, Animal; D055088:Early Detection of Cancer / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D009363:Neoplasm Proteins / Q000235:genetics* / Q000378:metabolism; D014411:Neoplastic Stem Cells / Q000378:metabolism / Q000473:pathology; D009780:Occult Blood; D057285:Precision Medicine; D040901:Proteomics / Q000295:instrumentation / Q000379:methods*; D057170:Translational Research, Biomedical
chemical_list: D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins
keywords: Biomarker; Colorectal cancer; MRM; MS; Translational proteomics