title: pH-Dependent Changes in the Mechanisms of Transport of Chlorine e6 and Its Derivatives in the Blood.
abstract: We studied the effects of medium pH on steady-state distribution of chlorine e6 and its derivatives between the main transport proteins of human blood plasma. The decrease in medium pH from weakly alkaline (pH 7.4) to acid (pH 5.0) was followed by an increase in relative affinity of chlorines to lipoproteins and reduced their affinity to serum albumin. pH-Dependent changes in the parameters of distribution of photosensitizers between the plasma and blood cells was revealed. We discussed the role of charge and polarity degree of photosensitizer molecule in the mechanism of binding to serum albumin. A possible role of changes in hydrogen ion activity in the processes of selective accumulation of chlorines by tumor cells is discussed.
authors.affiliation: Research Laboratory of Biophysics and Biotechnology, Department of Biophysics, Physical Faculty, Belorussian State University, Minsk, Republic of Belarus. ivan2khl@mail.ru.
authors.forename: I I
authors.initials: II
authors.lastname: Khludeev
authors.affiliation: Department of Physics and Mathematics, Pediatric Faculty, N. I. Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: L A
authors.initials: LA
authors.lastname: Kozyr'
authors.affiliation: Research Laboratory of Biophysics and Biotechnology, Department of Biophysics, Physical Faculty, Belorussian State University, Minsk, Republic of Belarus.
authors.forename: T E
authors.initials: TE
authors.lastname: Zorina
authors.affiliation: Research Laboratory of Biophysics and Biotechnology, Department of Biophysics, Physical Faculty, Belorussian State University, Minsk, Republic of Belarus.
authors.forename: V P
authors.initials: VP
authors.lastname: Zorin
mesh_terms: D001692:Biological Transport / Q000502:physiology; D002735:Chlorophyllides; D006801:Humans; D006863:Hydrogen-Ion Concentration; D011166:Porphyrins / Q000097:blood* / Q000378:metabolism*; D044967:Serum / Q000378:metabolism; D012709:Serum Albumin / Q000378:metabolism
chemical_list: D002735:Chlorophyllides; D011166:Porphyrins; D012709:Serum Albumin; C062985:phytochlorin
keywords: albumin; blood serum; chlorine e 6; lipoproteins; pH of medium