title: Evaluation of microleakage in class V composite restorations using dye penetration and micro-CT.
abstract: OBJECTIVE
  The aim of this study was the evaluation of microleakage in class V cavities using micro-CT imaging and dye penetration.
  
  
  METHODS
  Sixty class V cavities were prepared, randomly divided into five groups (Adper Single Bond Plus (ASB), ExciTE (EX), iBond TE (ITE), Optibond Solo Plus (OSP), Prime & Bond NT (PB)) and restored with Venus Diamond. After thermocycling and dye penetration test with 50 wt% ammoniacal silver nitrate, eight samples per group were sectioned longitudinally and evaluated under a coaxial incident light microscope (enamel (E) and dentin (D) measurement in mm). The remaining samples were analyzed by counting voids in the coronal and cervical adhesive areas by means of micro-CT.
  
  
  RESULTS
  In dentin, no statistically significant difference in dye penetration was found. In enamel, OSP showed significant higher microleakage than all other adhesives (p < 0.001). Groups ITE, ASB, PB, and EX showed a significantly lower penetration depth in E than in D (p < 0.001). The results of the micro-CT examination in dentin are contrary to the findings of the microleakage evaluation. Regarding enamel, only group PB showed significant more voids than EX in enamel (p < 0.02). Within the adhesive groups, PB showed significantly more voids in E than in D (p < 0.04).
  
  
  CONCLUSIONS
  This test method must be optimized by developing a new leakage tracer for a better differentiation between hard tissues and dental materials.
  
  
  CONCLUSIONS
  The micro-CT is not yet a vulnerable tool to evaluate the marginal integrity of resin composites.
authors.affiliation: Department of Conservative Dentistry, Periodontology and Preventive Dentistry, Hannover Medical School, 30625, Carl-Neuberg-Strasse 1, Hannover, Germany. Jacker-Guhr.Silke@mh-hannover.de.
authors.forename: S
authors.initials: S
authors.lastname: Jacker-Guhr
authors.affiliation: Department of Restorative Dentistry, Oregon Health and Science University, 3181 S.W. Sam Jackson Park Rd., Portland, OR, 97239-3098, USA.
authors.forename: G
authors.initials: G
authors.lastname: Ibarra
authors.affiliation: Department of Conservative Dentistry, Periodontology and Preventive Dentistry, Hannover Medical School, 30625, Carl-Neuberg-Strasse 1, Hannover, Germany.
authors.forename: L S
authors.initials: LS
authors.lastname: Oppermann
authors.affiliation: Department of Conservative Dentistry, Periodontology and Preventive Dentistry, Hannover Medical School, 30625, Carl-Neuberg-Strasse 1, Hannover, Germany.
authors.forename: A-K
authors.initials: AK
authors.lastname: Lührs
authors.affiliation: Department of Conservative Dentistry, Periodontology and Preventive Dentistry, Hannover Medical School, 30625, Carl-Neuberg-Strasse 1, Hannover, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Rahman
authors.affiliation: Department of Conservative Dentistry, Periodontology and Preventive Dentistry, Hannover Medical School, 30625, Carl-Neuberg-Strasse 1, Hannover, Germany.
authors.forename: W
authors.initials: W
authors.lastname: Geurtsen
mesh_terms: D004396:Coloring Agents; D003188:Composite Resins; D003737:Dental Cavity Preparation / Q000379:methods*; D003763:Dental Leakage / Q000000981:diagnostic imaging*; D003793:Dental Restoration, Permanent; D006801:Humans; D055114:X-Ray Microtomography
chemical_list: D004396:Coloring Agents; D003188:Composite Resins
keywords: Class V cavities; Dye penetration; Micro-CT; Microleakage