title: Expression pattern of bcar3, a downstream target of Gata2, and its binding partner, bcar1, during Xenopus development.
abstract: Primitive hematopoiesis generates red blood cells that deliver oxygen to the developing embryo. Mesodermal cells commit to a primitive blood cell fate during gastrulation and, in order to do so the mesoderm must receive non-cell autonomous signals transmitted from other germ layers. In Xenopus, the transcription factor Gata2 functions in ectodermal cells to generate or transmit the non-cell autonomous signals. Here we have identified Breast Cancer Antiestrogen Resistance 3 (bcar3) as a gene that is induced in ectodermal cells downstream of Gata2. Bcar3 and its binding partner Bcar1 function to transduce integrin signaling, leading to changes in cellular morphology, motility and adhesion. We show that gata2, bcar3 and bcar1 are co-expressed in ventral ectoderm from early gastrula to early tailbud stages. At later stages of development, bcar3 and bcar1 are co-expressed in the spinal cord, notochord, fin mesenchyme and pronephros but each shows additional unique sites of expression. These co-expression and unique expression patterns suggest that Bcar3 and Bcar1 may function together but also independently during Xenopus development.
authors.affiliation: Department of Neurobiology and Anatomy, Division of Hematology and Hematologic Malignancies, University of Utah, School of Medicine, 20 North 1900 East, Salt Lake City, UT 94132, USA; Department of Internal Medicine, Division of Hematology and Hematologic Malignancies, University of Utah, School of Medicine, 20 North 1900 East, Salt Lake City, UT 94132, USA.
authors.forename: Yangsook Song
authors.initials: YS
authors.lastname: Green
authors.affiliation: Department of Cell, Developmental and Cancer Biology, Oregon Health and Science University, School of Medicine, 3181 S.W. Sam Jackson Park Rd., Portland, OR 97239-3098, USA.
authors.forename: Sunjong
authors.initials: S
authors.lastname: Kwon
authors.affiliation: Department of Neurobiology and Anatomy, Division of Hematology and Hematologic Malignancies, University of Utah, School of Medicine, 20 North 1900 East, Salt Lake City, UT 94132, USA; Department of Internal Medicine, Division of Hematology and Hematologic Malignancies, University of Utah, School of Medicine, 20 North 1900 East, Salt Lake City, UT 94132, USA. Electronic address: jan.christian@neuro.utah.edu.
authors.forename: Jan L
authors.initials: JL
authors.lastname: Christian
mesh_terms: D000818:Animals; D004475:Ectoderm / Q000378:metabolism; D050989:GATA2 Transcription Factor / Q000235:genetics* / Q000378:metabolism; D005775:Gastrula / Q000378:metabolism*; D020662:Guanine Nucleotide Exchange Factors / Q000235:genetics* / Q000378:metabolism; D006410:Hematopoiesis; D046228:Microarray Analysis; D011485:Protein Binding; D015398:Signal Transduction; D046509:Tissue Culture Techniques; D029867:Xenopus Proteins / Q000235:genetics* / Q000378:metabolism; D014982:Xenopus laevis
chemical_list: C000610493:Bcar1 protein, Xenopus; C000610492:Bcar3 protein, Xenopus; D050989:GATA2 Transcription Factor; C494714:GATA2 protein, Xenopus; D020662:Guanine Nucleotide Exchange Factors; D029867:Xenopus Proteins
keywords: Bcar1; Bcar3; Ectoderm; Gastrulation; Gata2; Non-cell autonomous signals; Primitive hematopoiesis; Xenopus laevis