title: Fasudil, a Rho-Associated Protein Kinase Inhibitor, Attenuates Traumatic Retinal Nerve Injury in Rabbits.
abstract: Inhibition of RhoA/Rock could promote axon growth and alleviate optic nerve injury. However, the role of RhoA/Rock in the traumatic retinal nerve in vivo was not completely clear. In this study, we established a rabbit model of traumatic retinal nerve injury, and primary retinal ganglion cells (RGCs) were isolated and cultured under hypoxia-hypoglycemia condition that was mock to the microenvironment in the injured retinas in vivo. The Rock inhibitor fasudil was used to treat primary RGCs and ear vein injected into the model rabbits in vivo. RhoA/Rock signaling was activated in the injured optic nerve in rabbits. Western blotting analysis showed that RhoA/Rock signaling in the retina was activated during the traumatic optic neuropathy. Data on gene expression examination and Annexin V/PI dual staining combined with flow cytometry analysis displayed that fasudil injection reduced expression of Rho/Rock and apoptotic genes, as well as the apoptosis of RGCs in traumatic retinal nerve injury in vitro and in vivo. Moreover, fasudil injection reduced expression of Rho/Rock and apoptotic genes, as well as the apoptosis of RGCs in the rabbits with traumatic retinal nerve injury in vivo. In conclusion, fasudil treatment could significantly reduce the apoptosis of RGCs and relieved retinal nerve injury in vitro and in vivo.
authors.affiliation: Department of Neurosurgery, The Second Affiliated Hospital of Xinjiang Medical University, No.38 Nanhu East Road, Urumqi, 830063, Xinjiang, China.|Department of Neurosurgery, People's Hospital of Xinjiang Bazhou Region, Korla, 841000, Xinjiang, China.
authors.forename: Jianglong
authors.initials: J
authors.lastname: Yu
authors.affiliation: Department of Neurosurgery, The Second Affiliated Hospital of Xinjiang Medical University, No.38 Nanhu East Road, Urumqi, 830063, Xinjiang, China. xinpingluan163@163.com.
authors.forename: Xinping
authors.initials: X
authors.lastname: Luan
authors.affiliation: Department of Neurosurgery, People's Hospital of Xinjiang Bazhou Region, Korla, 841000, Xinjiang, China.
authors.forename: Shiying
authors.initials: S
authors.lastname: Lan
authors.affiliation: Department of Neurosurgery, The Second Affiliated Hospital of Xinjiang Medical University, No.38 Nanhu East Road, Urumqi, 830063, Xinjiang, China.
authors.forename: Baofeng
authors.initials: B
authors.lastname: Yan
authors.affiliation: Department of Neurosurgery, The Second Affiliated Hospital of Xinjiang Medical University, No.38 Nanhu East Road, Urumqi, 830063, Xinjiang, China.
authors.forename: Aba
authors.initials: A
authors.lastname: Maier
mesh_terms: D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use; D000818:Animals; D017209:Apoptosis; D002478:Cells, Cultured; D008297:Male; D020221:Optic Nerve Injuries / Q000188:drug therapy* / Q000378:metabolism; D047428:Protein Kinase Inhibitors / Q000494:pharmacology* / Q000627:therapeutic use; D011817:Rabbits; D012165:Retinal Ganglion Cells / Q000187:drug effects* / Q000378:metabolism; D020742:rhoA GTP-Binding Protein / Q000037:antagonists & inhibitors* / Q000378:metabolism
chemical_list: D047428:Protein Kinase Inhibitors; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D020742:rhoA GTP-Binding Protein; C049347:fasudil
keywords: Apoptosis; Retinal ganglion cells; RhoA/Rock signaling; Traumatic optic neuropathy