title: Development of a Loop-Mediated Isothermal Amplification Assay for Rapid and Specific Identification of ACT Producing Alternaria alternata, the Agent of Brown Spot Disease in Tangerine.
abstract: Rapid, accurate, and easy identification of pathogenic agents has always been important in medicine, veterinary, and agriculture. The brown spot infection is among the most common diseases in tangerine caused by Alternaria alternata. Due to the existence of seven various pathotypes of A. alternata species, it is challenging and time consuming to detect a pathotype responsible for citrus brown spot. In this study, we were seeking a rapid and specific approach to identify the tangerine pathotype within the A. alternata-pathogenic species, using the loop-mediated isothermal amplification (LAMP) method and actts2 gene as a marker molecule. Nine pathogenic samples were obtained from the region of Ramsar, Iran, and certified as A. alternata-pathogenic isolates. Specific primers were designed for regions coding for Alternaria citri toxin (ACT), and the PCR and LAMP reactions were performed. Our data showed that the primers designed for the tangerine pathotype of A. alternata were specific, and in both reactions, positive results were only observed in desired pathotypes. In the other pathotypes of this species as well as other standard fungal samples as negative controls, no positive result was observed. Therefore, our results suggest the possibility to detect the tangerine-specific A. alternata pathotype from other related species with a high accuracy and in early stages of the disease.
authors.affiliation: Department of Microbial Biotechnology, School of Biology and Center of Excellence in Phylogeny of Living Organisms, College of Science, University of Tehran, Enghelab Ave., Tehran, 141556455, Iran. hmoghimi@ut.ac.ir.|Microbial Technology and Products Research Center, University of Tehran, Ghods St., Enghelab Ave., Tehran, 1417864411, Iran. hmoghimi@ut.ac.ir.
authors.forename: Hamid
authors.initials: H
authors.lastname: Moghimi
authors.affiliation: Department of Microbial Biotechnology, School of Biology and Center of Excellence in Phylogeny of Living Organisms, College of Science, University of Tehran, Enghelab Ave., Tehran, 141556455, Iran.|Microbial Technology and Products Research Center, University of Tehran, Ghods St., Enghelab Ave., Tehran, 1417864411, Iran.
authors.forename: Amir
authors.initials: A
authors.lastname: Moradi
authors.affiliation: Department of Microbial Biotechnology, School of Biology and Center of Excellence in Phylogeny of Living Organisms, College of Science, University of Tehran, Enghelab Ave., Tehran, 141556455, Iran.|Microbial Technology and Products Research Center, University of Tehran, Ghods St., Enghelab Ave., Tehran, 1417864411, Iran.
authors.forename: Javad
authors.initials: J
authors.lastname: Hamedi
authors.affiliation: Department of Microbial Biotechnology, School of Biology and Center of Excellence in Phylogeny of Living Organisms, College of Science, University of Tehran, Enghelab Ave., Tehran, 141556455, Iran.|Microbial Technology and Products Research Center, University of Tehran, Ghods St., Enghelab Ave., Tehran, 1417864411, Iran.
authors.forename: Mina
authors.initials: M
authors.lastname: Basiri
mesh_terms: D000528:Alternaria / Q000302:isolation & purification / Q000378:metabolism* / Q000472:pathogenicity; D002957:Citrus / Q000382:microbiology*; D009181:Mycoses / Q000382:microbiology*; D010935:Plant Diseases / Q000382:microbiology*
keywords: ACT; Brown spot; Citrus fungal disease; LAMP; Molecular detection