title: Towards the development of an oral vaccine against porcine cysticercosis: expression of the protective HP6/TSOL18 antigen in transgenic carrots cells.
abstract: The Taenia solium HP6/TSOL18 antigen was produced in carrot cells, yielding an immunogenic protein that induced significant protection in an experimental murine model against T. crassiceps cysticercosis when orally administered. This result supports the potential of HP6/TSOL18-carrot as a low-cost anti-cysticercosis vaccine candidate. Cysticercosis is a zoonosis caused by Taenia solium that can be prevented by interrupting the parasite life cycle through pig vaccination. Several injectable vaccine candidates have been reported, but the logistic difficulties and costs for its application limited its use in nationwide control programs. Oral plant-based vaccines can deal with this limitation, because of their easy administration and low cost. A stable expression of the HP6/TSOL18 anti-T. solium cysticercosis protective antigen in carrot calli transformed with an optimized transgene is herein reported. An antigen accumulation up to 14 µg g(-1) of dry-weight biomass was achieved in the generated carrot lines. Mouse immunization with one of the transformed calli induced both specific IgG and IgA anti-HP6/TSOL18 antibodies. A statistically significant reduction in the expected number of T. crassiceps cysticerci was observed in mice orally immunized with carrot-made HP6/TSOL18, in a similar extent to that obtained by subcutaneous immunization with recombinant HP6/TSOL18 protein. In this study, a new oral plant-made version of the HP6/TSOL18 anti-cysticercosis vaccine is reported. The vaccine candidate should be further tested against porcine cysticercosis.
authors.affiliation: Laboratorio de Biofarmacéuticos Recombinantes, Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí, Av. Dr. Manuel Nava 6, 78210, San Luis Potosí, Mexico.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Monreal-Escalante
authors.affiliation: Laboratorio de Biofarmacéuticos Recombinantes, Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí, Av. Dr. Manuel Nava 6, 78210, San Luis Potosí, Mexico.
authors.forename: Dania O
authors.initials: DO
authors.lastname: Govea-Alonso
authors.affiliation: Dpto. Inmunología. Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Circuito Escolar, Ciudad Universitaria, 04510, Mexico, D.F., Mexico.
authors.forename: Marisela
authors.initials: M
authors.lastname: Hernández
authors.affiliation: Dpto. Inmunología. Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Circuito Escolar, Ciudad Universitaria, 04510, Mexico, D.F., Mexico.
authors.forename: Jacquelynne
authors.initials: J
authors.lastname: Cervantes
authors.affiliation: Laboratorio de Biofarmacéuticos Recombinantes, Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí, Av. Dr. Manuel Nava 6, 78210, San Luis Potosí, Mexico.
authors.forename: Jorge A
authors.initials: JA
authors.lastname: Salazar-González
authors.affiliation: Laboratorio de Biofarmacéuticos Recombinantes, Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí, Av. Dr. Manuel Nava 6, 78210, San Luis Potosí, Mexico.
authors.forename: Andrea
authors.initials: A
authors.lastname: Romero-Maldonado
authors.affiliation: Facultad de Medicina, Universidad Autónoma del Estado de Morelos, Cuernavaca, Mexico.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Rosas
authors.affiliation: Dpto. de Parasitología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, 28220, Madrid, Spain.
authors.forename: Teresa
authors.initials: T
authors.lastname: Garate
authors.affiliation: Dpto. Inmunología. Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Circuito Escolar, Ciudad Universitaria, 04510, Mexico, D.F., Mexico.
authors.forename: Gladis
authors.initials: G
authors.lastname: Fragoso
authors.affiliation: Dpto. Inmunología. Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Circuito Escolar, Ciudad Universitaria, 04510, Mexico, D.F., Mexico. edda@unam.mx.
authors.forename: Edda
authors.initials: E
authors.lastname: Sciutto
authors.affiliation: Laboratorio de Biofarmacéuticos Recombinantes, Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí, Av. Dr. Manuel Nava 6, 78210, San Luis Potosí, Mexico. rosales.s@fcq.uaslp.mx.
authors.forename: Sergio
authors.initials: S
authors.lastname: Rosales-Mendoza
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology*; D003551:Cysticercosis / Q000469:parasitology / Q000517:prevention & control / Q000662:veterinary*; D018552:Daucus carota / Q000235:genetics / Q000378:metabolism*; D005260:Female; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C; D011994:Recombinant Proteins; D013552:Swine; D041201:Taenia solium / Q000276:immunology*; D019076:Transgenes; D014612:Vaccines
chemical_list: D000947:Antigens, Helminth; D011994:Recombinant Proteins; C113407:TSOL-18 antigen; D014612:Vaccines
keywords: Cysticercosis; Delivery system; HP6/TSOL18; Oral vaccine; Plant-based vaccine; Taenia; Vaccination