title: Genome-Wide Screening of mRNA Expression in Leprosy Patients.
abstract: Leprosy, an infectious disease caused by Mycobacterium leprae, affects millions of people worldwide. However, little is known regarding its molecular pathophysiological mechanisms. In this study, a comprehensive assessment of human mRNA was performed on leprosy skin lesions by using DNA chip microarrays, which included the entire spectrum of the disease along with its reactional states. Sixty-six samples from leprotic lesions (10TT, 10BT, 10BB, 10BL, 4LL, 14R1, and 10R2) and nine skin biopsies from healthy individuals were used as controls (CC) (ages ranged from 06 to 83 years, 48 were male and 29 female). The evaluation identified 1580 differentially expressed mRNAs [Fold Change (FC) ≥ 2.0, p ≤ 0.05] in diseased lesions vs. healthy controls. Some of these genes were observed in all forms of the disease (CD2, CD27, chit1, FA2H, FAM26F, GZMB, MMP9, SLAMF7, UBD) and others were exclusive to reactional forms (Type "1" reaction: GPNMB, IL1B, MICAL2, FOXQ1; Type "2" reaction: AKR1B10, FAM180B, FOXQ1, NNMT, NR1D1, PTX3, TNFRSF25). In literature, these mRNAs have been associated with numerous pathophysiological processes and signaling pathways and are present in a large number of diseases. The role of these mRNAs maybe studied in the context of developing new diagnostic markers and therapeutic targets for leprosy.
authors.affiliation: Department of Anatomic Pathology, Instituto Lauro de Souza Lima São Paulo, Brazil ; Department of Anatomic Pathology, A.C. Camargo Cancer Center São Paulo, Brazil.
authors.forename: Andrea de Faria F
authors.initials: Ade F
authors.lastname: Belone
authors.affiliation: Division of Research and Education, Instituto Lauro de Souza Lima São Paulo, Brazil.
authors.forename: Patrícia S
authors.initials: PS
authors.lastname: Rosa
authors.affiliation: Department of Health Science, Universidade do Sagrado Coração São Paulo, Brazil.
authors.forename: Ana P F
authors.initials: AP
authors.lastname: Trombone
authors.affiliation: Department of Anatomic Pathology, Instituto Lauro de Souza Lima São Paulo, Brazil.
authors.forename: Luciana R V
authors.initials: LR
authors.lastname: Fachin
authors.affiliation: Ambulatory of Leprosy, Jardim Guanabara Health Center Rondonópolis, Brazil.
authors.forename: Cássio C
authors.initials: CC
authors.lastname: Guidella
authors.affiliation: Division of Research and Education, Instituto Lauro de Souza Lima São Paulo, Brazil.
authors.forename: Somei
authors.initials: S
authors.lastname: Ura
authors.affiliation: Division of Dermathology, Instituto Lauro de Souza Lima São Paulo, Brazil.
authors.forename: Jaison A
authors.initials: JA
authors.lastname: Barreto
authors.affiliation: Laboratory of Genomics and Molecular Biology, CIPE, A.C. Camargo Cancer Center Sao Paulo, Brazil.
authors.forename: Mabel G
authors.initials: MG
authors.lastname: Pinilla
authors.affiliation: Laboratory of Genomics and Molecular Biology, CIPE, A.C. Camargo Cancer Center Sao Paulo, Brazil.
authors.forename: Alex F
authors.initials: AF
authors.lastname: de Carvalho
authors.affiliation: Laboratory of Genomics and Molecular Biology, CIPE, A.C. Camargo Cancer Center Sao Paulo, Brazil.
authors.forename: Dirce M
authors.initials: DM
authors.lastname: Carraro
authors.affiliation: Department of Anatomic Pathology, A.C. Camargo Cancer Center São Paulo, Brazil.
authors.forename: Fernando A
authors.initials: FA
authors.lastname: Soares
authors.affiliation: Department of Anatomic Pathology, Instituto Lauro de Souza Lima São Paulo, Brazil.
authors.forename: Cleverson T
authors.initials: CT
authors.lastname: Soares
keywords: immunohistochemistry; leprosy; mRNA; microarray; signaling pathways