title: Control of growth factor binding and release in bisphosphonate functionalized hydrogels guides rapid differentiation of precursor cells in vitro.
abstract: An in situ cross-linkable hyaluronan hydrogel functionalized with bisphosphonate (BP) groups allows tunable release of bone morphogenetic protein-2 (BMP-2) determined by the amount of BP groups. The high affinity of matrix-anchored BP groups towards BMP-2 permits guided differentiation of entrapped progenitor cells in 3-D cultures.
authors.affiliation: Science for Life Laboratory, Department of Chemistry-Ångström Laboratory, Uppsala University, Uppsala, Sweden. dmitri.ossipov@kemi.uu.se.
authors.forename: Sujit
authors.initials: S
authors.lastname: Kootala
authors.affiliation: Science for Life Laboratory, Department of Chemistry-Ångström Laboratory, Uppsala University, Uppsala, Sweden. dmitri.ossipov@kemi.uu.se.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Science for Life Laboratory, Department of Chemistry-Ångström Laboratory, Uppsala University, Uppsala, Sweden. dmitri.ossipov@kemi.uu.se.
authors.forename: Sara
authors.initials: S
authors.lastname: Ghalib
authors.affiliation: Unit of Biomedical Radiation Sciences, Rudbeck Laboratory, Uppsala University, S-75121 Uppsala, Sweden.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Tolmachev
authors.affiliation: Science for Life Laboratory, Department of Chemistry-Ångström Laboratory, Uppsala University, Uppsala, Sweden. dmitri.ossipov@kemi.uu.se.
authors.forename: Jöns
authors.initials: J
authors.lastname: Hilborn
authors.affiliation: Science for Life Laboratory, Department of Chemistry-Ångström Laboratory, Uppsala University, Uppsala, Sweden. dmitri.ossipov@kemi.uu.se.
authors.forename: Dmitri A
authors.initials: DA
authors.lastname: Ossipov
mesh_terms: D055396:Bone Morphogenetic Protein 2 / Q000737:chemistry* / Q000378:metabolism; D002454:Cell Differentiation; D004164:Diphosphonates / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry*; D020100:Hydrogels / Q000737:chemistry*
chemical_list: C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D004164:Diphosphonates; D020100:Hydrogels; D006820:Hyaluronic Acid