title: Concentric-flow electrokinetic injector enables serial crystallography of ribosome and photosystem II.
abstract: We describe a concentric-flow electrokinetic injector for efficiently delivering microcrystals for serial femtosecond X-ray crystallography analysis that enables studies of challenging biological systems in their unadulterated mother liquor. We used the injector to analyze microcrystals of Geobacillus stearothermophilus thermolysin (2.2-Å structure), Thermosynechococcus elongatus photosystem II (<3-Å diffraction) and Thermus thermophilus small ribosomal subunit bound to the antibiotic paromomycin at ambient temperature (3.4-Å structure).
authors.affiliation: Stanford PULSE Institute, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
authors.forename: Raymond G
authors.initials: RG
authors.lastname: Sierra
authors.affiliation: Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron, Hamburg, Germany.
authors.forename: Cornelius
authors.initials: C
authors.lastname: Gati
authors.affiliation: Stanford PULSE Institute, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
authors.forename: Hartawan
authors.initials: H
authors.lastname: Laksmono
authors.affiliation: Stanford PULSE Institute, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
authors.forename: E Han
authors.initials: EH
authors.lastname: Dao
authors.affiliation: Lawrence Berkeley National Laboratory, Berkeley, California, USA.
authors.forename: Sheraz
authors.initials: S
authors.lastname: Gul
authors.affiliation: Lawrence Berkeley National Laboratory, Berkeley, California, USA.
authors.forename: Franklin
authors.initials: F
authors.lastname: Fuller
authors.affiliation: Lawrence Berkeley National Laboratory, Berkeley, California, USA.|Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
authors.forename: Jan
authors.initials: J
authors.lastname: Kern
authors.affiliation: Lawrence Berkeley National Laboratory, Berkeley, California, USA.
authors.forename: Ruchira
authors.initials: R
authors.lastname: Chatterjee
authors.affiliation: Institute für Biologie, Humboldt University of Berlin, Berlin, Germany.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Ibrahim
authors.affiliation: Lawrence Berkeley National Laboratory, Berkeley, California, USA.
authors.forename: Aaron S
authors.initials: AS
authors.lastname: Brewster
authors.affiliation: Lawrence Berkeley National Laboratory, Berkeley, California, USA.
authors.forename: Iris D
authors.initials: ID
authors.lastname: Young
authors.affiliation: Lawrence Berkeley National Laboratory, Berkeley, California, USA.
authors.forename: Tara
authors.initials: T
authors.lastname: Michels-Clark
authors.affiliation: Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
authors.forename: Andrew
authors.initials: A
authors.lastname: Aquila
authors.affiliation: Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
authors.forename: Mengning
authors.initials: M
authors.lastname: Liang
authors.affiliation: Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
authors.forename: Mark S
authors.initials: MS
authors.lastname: Hunter
authors.affiliation: Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
authors.forename: Jason E
authors.identifier: http://orcid.org/0000-0001-6811-6083
authors.initials: JE
authors.lastname: Koglin
authors.affiliation: Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Boutet
authors.affiliation: Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
authors.forename: Elia A
authors.initials: EA
authors.lastname: Junco
authors.affiliation: Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
authors.forename: Brandon
authors.initials: B
authors.lastname: Hayes
authors.affiliation: Stanford PULSE Institute, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Bogan
authors.affiliation: Stanford PULSE Institute, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
authors.forename: Christina Y
authors.initials: CY
authors.lastname: Hampton
authors.affiliation: Department of Structural Biology, Stanford University School of Medicine, Stanford, California, USA.
authors.forename: Elisabetta V
authors.initials: EV
authors.lastname: Puglisi
authors.affiliation: Lawrence Berkeley National Laboratory, Berkeley, California, USA.
authors.forename: Nicholas K
authors.initials: NK
authors.lastname: Sauter
authors.affiliation: Stanford PULSE Institute, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
authors.forename: Claudiu A
authors.initials: CA
authors.lastname: Stan
authors.affiliation: Institute für Biologie, Humboldt University of Berlin, Berlin, Germany.
authors.forename: Athina
authors.initials: A
authors.lastname: Zouni
authors.affiliation: Lawrence Berkeley National Laboratory, Berkeley, California, USA.
authors.forename: Junko
authors.initials: J
authors.lastname: Yano
authors.affiliation: Lawrence Berkeley National Laboratory, Berkeley, California, USA.
authors.forename: Vittal K
authors.initials: VK
authors.lastname: Yachandra
authors.affiliation: Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
authors.forename: S Michael
authors.initials: SM
authors.lastname: Soltis
authors.affiliation: Department of Structural Biology, Stanford University School of Medicine, Stanford, California, USA.
authors.forename: Joseph D
authors.initials: JD
authors.lastname: Puglisi
authors.affiliation: Stanford PULSE Institute, SLAC National Accelerator Laboratory, Menlo Park, California, USA.|Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
authors.forename: Hasan
authors.identifier: http://orcid.org/0000-0002-9135-5397
authors.initials: H
authors.lastname: DeMirci
mesh_terms: D003461:Crystallography / Q000379:methods*; D008958:Models, Molecular; D045332:Photosystem II Protein Complex / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism*
chemical_list: D045332:Photosystem II Protein Complex