title: Gut Commensal E. coli Proteins Activate Host Satiety Pathways following Nutrient-Induced Bacterial Growth.
abstract: The composition of gut microbiota has been associated with host metabolic phenotypes, but it is not known if gut bacteria may influence host appetite. Here we show that regular nutrient provision stabilizes exponential growth of E. coli, with the stationary phase occurring 20 min after nutrient supply accompanied by bacterial proteome changes, suggesting involvement of bacterial proteins in host satiety. Indeed, intestinal infusions of E. coli stationary phase proteins increased plasma PYY and their intraperitoneal injections suppressed acutely food intake and activated c-Fos in hypothalamic POMC neurons, while their repeated administrations reduced meal size. ClpB, a bacterial protein mimetic of α-MSH, was upregulated in the E. coli stationary phase, was detected in plasma proportional to ClpB DNA in feces, and stimulated firing rate of hypothalamic POMC neurons. Thus, these data show that bacterial proteins produced after nutrient-induced E. coli growth may signal meal termination. Furthermore, continuous exposure to E. coli proteins may influence long-term meal pattern.
authors.affiliation: Inserm UMR1073, Nutrition, Gut and Brain Laboratory, Rouen 76183, France; Institute for Research and Innovation in Biomedicine (IRIB), Rouen University, Normandy University, Rouen 76000, France.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Breton
authors.affiliation: Inserm UMR1073, Nutrition, Gut and Brain Laboratory, Rouen 76183, France; Institute for Research and Innovation in Biomedicine (IRIB), Rouen University, Normandy University, Rouen 76000, France.
authors.forename: Naouel
authors.initials: N
authors.lastname: Tennoune
authors.affiliation: Inserm UMR1073, Nutrition, Gut and Brain Laboratory, Rouen 76183, France; Institute for Research and Innovation in Biomedicine (IRIB), Rouen University, Normandy University, Rouen 76000, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Lucas
authors.affiliation: Inserm UMR1073, Nutrition, Gut and Brain Laboratory, Rouen 76183, France; Institute for Research and Innovation in Biomedicine (IRIB), Rouen University, Normandy University, Rouen 76000, France.
authors.forename: Marie
authors.initials: M
authors.lastname: Francois
authors.affiliation: Inserm UMR1073, Nutrition, Gut and Brain Laboratory, Rouen 76183, France; Institute for Research and Innovation in Biomedicine (IRIB), Rouen University, Normandy University, Rouen 76000, France.
authors.forename: Romain
authors.initials: R
authors.lastname: Legrand
authors.affiliation: Inserm UMR1073, Nutrition, Gut and Brain Laboratory, Rouen 76183, France; Institute for Research and Innovation in Biomedicine (IRIB), Rouen University, Normandy University, Rouen 76000, France.
authors.forename: Justine
authors.initials: J
authors.lastname: Jacquemot
authors.affiliation: Inserm UMR1073, Nutrition, Gut and Brain Laboratory, Rouen 76183, France; Institute for Research and Innovation in Biomedicine (IRIB), Rouen University, Normandy University, Rouen 76000, France.
authors.forename: Alexis
authors.initials: A
authors.lastname: Goichon
authors.affiliation: Inserm UMR1073, Nutrition, Gut and Brain Laboratory, Rouen 76183, France; Institute for Research and Innovation in Biomedicine (IRIB), Rouen University, Normandy University, Rouen 76000, France.
authors.forename: Charlène
authors.initials: C
authors.lastname: Guérin
authors.affiliation: Microbiology Laboratory GRAM, EA2656, Rouen 76183, France; Institute for Research and Innovation in Biomedicine (IRIB), Rouen University, Normandy University, Rouen 76000, France.
authors.forename: Johann
authors.initials: J
authors.lastname: Peltier
authors.affiliation: Microbiology Laboratory GRAM, EA2656, Rouen 76183, France; Institute for Research and Innovation in Biomedicine (IRIB), Rouen University, Normandy University, Rouen 76000, France; Rouen University Hospital, CHU Charles Nicolle, Rouen 76183, France.
authors.forename: Martine
authors.initials: M
authors.lastname: Pestel-Caron
authors.affiliation: PISSARO Proteomic Platform, Mont-Saint-Aignan 76821, France; Institute for Research and Innovation in Biomedicine (IRIB), Rouen University, Normandy University, Rouen 76000, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Chan
authors.affiliation: PISSARO Proteomic Platform, Mont-Saint-Aignan 76821, France; Institute for Research and Innovation in Biomedicine (IRIB), Rouen University, Normandy University, Rouen 76000, France.
authors.forename: David
authors.initials: D
authors.lastname: Vaudry
authors.affiliation: Animal Behavior Platform (SCAC), Rouen 76183, France; Institute for Research and Innovation in Biomedicine (IRIB), Rouen University, Normandy University, Rouen 76000, France.
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: do Rego
authors.affiliation: Centre for Taste and Feeding Behaviour, UMR 6265-CNRS, 1324-INRA, Bourgogne Franche Comté University, Dijon F 21000, France.
authors.forename: Fabienne
authors.initials: F
authors.lastname: Liénard
authors.affiliation: Centre for Taste and Feeding Behaviour, UMR 6265-CNRS, 1324-INRA, Bourgogne Franche Comté University, Dijon F 21000, France.
authors.forename: Luc
authors.initials: L
authors.lastname: Pénicaud
authors.affiliation: Centre for Taste and Feeding Behaviour, UMR 6265-CNRS, 1324-INRA, Bourgogne Franche Comté University, Dijon F 21000, France.
authors.forename: Xavier
authors.initials: X
authors.lastname: Fioramonti
authors.affiliation: Neuropharmacology Research Group, School of Pharmacy and Biomedical Sciences University of Portsmouth, Portsmouth PO 1 2DT, UK.
authors.forename: Ivor S
authors.initials: IS
authors.lastname: Ebenezer
authors.affiliation: Department of Neuroscience, Karolinska Institutet, Stockholm 17176, Sweden.
authors.forename: Tomas
authors.initials: T
authors.lastname: Hökfelt
authors.affiliation: Inserm UMR1073, Nutrition, Gut and Brain Laboratory, Rouen 76183, France; Institute for Research and Innovation in Biomedicine (IRIB), Rouen University, Normandy University, Rouen 76000, France; Rouen University Hospital, CHU Charles Nicolle, Rouen 76183, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Déchelotte
authors.affiliation: Inserm UMR1073, Nutrition, Gut and Brain Laboratory, Rouen 76183, France; Institute for Research and Innovation in Biomedicine (IRIB), Rouen University, Normandy University, Rouen 76000, France. Electronic address: serguei.fetissov@univ-rouen.fr.
authors.forename: Sergueï O
authors.initials: SO
authors.lastname: Fetissov
mesh_terms: D000255:Adenosine Triphosphate / Q000096:biosynthesis; D000679:Amygdala / Q000378:metabolism; D000818:Animals; D055724:Electrophysiological Phenomena; D049071:Endopeptidase Clp; D004926:Escherichia coli / Q000254:growth & development* / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000378:metabolism*; D005247:Feeding Behavior; D005260:Female; D041981:Gastrointestinal Tract / Q000382:microbiology*; D052216:Glucagon-Like Peptide 1 / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000378:metabolism; D007031:Hypothalamus / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000378:metabolism; D019894:Peptide YY / Q000378:metabolism; D011333:Pro-Opiomelanocortin / Q000378:metabolism; D040901:Proteomics; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D012528:Satiety Response
chemical_list: D029968:Escherichia coli Proteins; D006360:Heat-Shock Proteins; D016760:Proto-Oncogene Proteins c-fos; D019894:Peptide YY; D011333:Pro-Opiomelanocortin; D052216:Glucagon-Like Peptide 1; D000255:Adenosine Triphosphate; D049071:Endopeptidase Clp; C069775:ClpB protein, E coli