title: GM130 regulates epithelial-to-mesenchymal transition and invasion of gastric cancer cells via snail.
abstract: Gastric cancer is one of the most common causes of digestive tract tumor. Despite of recent advances in surgical techniques and development of adjuvant therapy, the underlying mechanisms of gastric cancer remain poorly understood and relevant insight into novel treatment strategies using gene target remains incomplete. Recently, several studies report that epithelial to mesenchymal transition (EMT) is a crucial process for the invasion and metastasis of epithelial tumors; however, the molecular mechanisms underlying this transition are unknown. As a cis-Golgi matrix protein, GM130 plays an important role in cell cycle progression and transport of protein in the secretory pathway. In this study, we found that GM130 expression has a positive correlation with the pathological differentiation and tumor node metastasis (TNM) stage of gastric cancer. High GM130 expression levels also predict shorter overall survival of gastric cancer patients. RNA interference-mediated knockdown of GM130 expression increased epithelial marker (E-cadherin) and decreased mesenchymal marker (N-cadherin and vimentin) expression in gastric cancer cells, suppressing cell invasion, and tumor formation. Furthermore, we found that GM130 upregulated expression of the key EMT regulator Snail (SNAI1), which mediated EMT activation and cell invasion by GM130. Taken together, our study indicates GM130 may be a promising therapeutic biomarker for gastric cancer.
authors.affiliation: Department of Cardiology, Bayannaoer City Hospital Inner Mongolia, China.
authors.forename: Jianquan
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Department of Medical Cosmetology Bayannaoer City Hospital Inner Mongolia, China.
authors.forename: Chun
authors.initials: C
authors.lastname: Yang
authors.affiliation: Department of Cardiology, Bayannaoer City Hospital Inner Mongolia, China.
authors.forename: Shujun
authors.initials: S
authors.lastname: Guo
authors.affiliation: Department of Orthopedics, Bayannaoer City Hospital Inner Mongolia, China.
authors.forename: Yonggang
authors.initials: Y
authors.lastname: Wu
mesh_terms: D015703:Antigens, CD / Q000378:metabolism; D001324:Autoantigens / Q000235:genetics / Q000378:metabolism*; D001665:Binding Sites; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D015820:Cadherins / Q000378:metabolism; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D002465:Cell Movement; D058750:Epithelial-Mesenchymal Transition; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053208:Kaplan-Meier Estimate; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D011401:Promoter Regions, Genetic; D034622:RNA Interference; D015398:Signal Transduction; D000071250:Snail Family Transcription Factors; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000401:mortality / Q000473:pathology / Q000628:therapy; D013997:Time Factors; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014162:Transfection; D014746:Vimentin / Q000378:metabolism
chemical_list: D015703:Antigens, CD; D001324:Autoantigens; D014408:Biomarkers, Tumor; C513384:CDH1 protein, human; C050361:CDH2 protein, human; D015820:Cadherins; C097741:Golgin subfamily A member 2; D008565:Membrane Proteins; C000604407:SNAI1 protein, human; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors; D014746:Vimentin
keywords: EMT; GM130; gastric cancer; invasion; migration