title: Vitamin C inhibit the proliferation, migration and epithelial-mesenchymal-transition of lens epithelial cells by destabilizing HIF-1α.
abstract: Posterior capsular opacification (PCO), the main complication of cataract surgery, is mainly caused by the proliferation, migration, and epithelial-mesenchymal transition (EMT) of the residual lens epithelial cells (LECs).Vitamin C was reported to reduce the risk of forming a cataract. However, there has been no study showing the association between vitamin C and PCO. In this study, we found that vitamin C could inhibit the migration and proliferation of human lens epithelial cells. We also found that vitamin C could increase the proline hydroxylation of HIF-1α and reduce the activity of HIF-1α. Moreover, vitamin C could not inhibit the activity of proline-mutant HIF-1α (402/564). Overexpression of wild-type HIF-1α or proline-mutant HIF-1α was found to increase the proliferation and migration of human lens epithelial cells. Differently, vitamin C could inhibit the proliferation and migration in wild-type HIF-1α-overexpressing lens epithelial cells but not the proline-mutant HIF-1α-overexpressing cells. Additionally, vitamin C was also found to inhibit the expression of EMT transcription factors TWIST. We then found that vitamin C could repress the EMT phenotypes induced by the overexpression of wild-type HIF-1α but not the proline-mutant HIF-1α. These results provide evidence that vitamin C plays a role in the repression of proliferation, migration, and EMT of human lens epithelial cells by destabilizing HIF-1α.
authors.affiliation: Department of Ophthalmology, The Second Affiliated Hospital of Xi'an Jiaotong University Xi'an 710004, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Department of Ophthalmology, The Second Affiliated Hospital of Xi'an Jiaotong University Xi'an 710004, China.
authors.forename: Yanlong
authors.initials: Y
authors.lastname: Quan
authors.affiliation: Department of Ophthalmology, The Second Affiliated Hospital of Xi'an Jiaotong University Xi'an 710004, China.
authors.forename: Jianming
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Ophthalmology, The Second Affiliated Hospital of Xi'an Jiaotong University Xi'an 710004, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.affiliation: Department of Ophthalmology, The Second Affiliated Hospital of Xi'an Jiaotong University Xi'an 710004, China.
authors.forename: Yuping
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Department of Ophthalmology, The Second Affiliated Hospital of Xi'an Jiaotong University Xi'an 710004, China.
authors.forename: Aiyi
authors.initials: A
authors.lastname: Zhou
keywords: HIF-1α; Lens epithelial cells; Vitamin C; epithelial-mesenchymal-transition; migration; posterior capsular opacification; proliferation