title: Manipulation of the ATP pool as a tool for metabolic engineering.
abstract: Cofactor engineering has been long identified as a valuable tool for metabolic engineering. Besides interventions targeting the pools of redox cofactors, many studies addressed the adenosine pools of microorganisms. In this mini-review, we discuss interventions that manipulate the availability of ATP with a special focus on ATP wasting strategies. We discuss the importance to fine-tune the ATP yield along a production pathway to balance process performance parameters like product yield and volumetric productivity.
authors.affiliation: Max Planck Institute for Dynamics of Complex Technical Systems, Sandtorstrasse 1, Magdeburg, 39106, Germany haedicke@mpi-magdeburg.mpg.de.
authors.forename: Oliver
authors.initials: O
authors.lastname: Hädicke
authors.affiliation: Max Planck Institute for Dynamics of Complex Technical Systems, Sandtorstrasse 1, Magdeburg, 39106, Germany.
authors.forename: Steffen
authors.initials: S
authors.lastname: Klamt
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D004734:Energy Metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D060847:Metabolic Engineering / Q000379:methods*; D053858:Metabolic Networks and Pathways; D008954:Models, Biological; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*
chemical_list: D000255:Adenosine Triphosphate
keywords: adenosine 5′-triphosphate (ATP) futile cycling; metabolic engineering; product yield; volumetric productivity