title: Helicobacter pylori antigens, acetylsalicylic acid, LDL and 7-ketocholesterol - their potential role in destabilizing the gastric epithelial cell barrier. An in vitro model of Kato III cells.
abstract: Colonization of gastric tissue in humans by H. pylori Gram-negative bacteria initiates gastric and duodenal ulcers and even gastric cancers. Infections promote inflammation and damage to gastric epithelium which might be followed by the impairment of its barrier function. The role of H. pylori components in these processes has not been specified. H. pylori cytotoxicity may potentially increase in the milieu of anti-inflammatory drugs including acetylsalicylic acid (ASA). The lipid transport-associated molecule such as low density lipoprotein (LDL), which is a classic risk factor of coronary heart disease (CHD) and 7-ketocholesterol (7-kCh) a product of cholesterol oxidation, which may occur during the oxidative stress in LDL could also be considered as pro-inflammatory. The aim of this study was to evaluate the cytotoxicity of H. pylori antigens, ASA, LDL and 7-kCh towards Kato III gastric epithelial cells, on the basis of the cell ability to reduce tetrazolium salt (MTT) and morphology of cell nuclei assessed by 4',6-diamidino-2-phenylindole (DAPI) staining. Kato III cells were stimulated for 24 h, at 37°C and 5% CO2, with H. pylori antigens: cytotoxin associated gene A (CagA) protein, the urease A subunit (UreA), lipopolysaccharide (LPS) and ASA, LDL or 7-kCh. H. pylori LPS, ASA, LDL and 7-kCh, but not H. pylori glycine acid extract (GE), demonstrated cytotoxicity against Kato III cells, which was related to a diminished percentage of MTT reducing cells and to an increased cell population with the signs of DNA damage. The results suggest that damage to gastric epithelial cells can be induced independently by H. pylori antigens, ASA and endogenous lipid transport-associated molecules. During H. pylori infection in vivo, especially in CHD patients, synergistic or antagonistic interactions between these factors might possibly influence the disease course. Further study is necessary to explain these potential effects.
authors.affiliation: Division of Gastroimmunology, Department of Immunology and Infectious Biology, Institute of Microbiology, Biotechnology and Immunology, Faculty of Biology and Environmental Protection, University of Lodz, Łódź, Poland.
authors.forename: Adrian
authors.initials: A
authors.lastname: Gajewski
authors.affiliation: Division of Gastroimmunology, Department of Immunology and Infectious Biology, Institute of Microbiology, Biotechnology and Immunology, Faculty of Biology and Environmental Protection, University of Lodz, Łódź, Poland.
authors.forename: Eliza
authors.initials: E
authors.lastname: Mnich
authors.affiliation: Division of Gastroimmunology, Department of Immunology and Infectious Biology, Institute of Microbiology, Biotechnology and Immunology, Faculty of Biology and Environmental Protection, University of Lodz, Łódź, Poland.
authors.forename: Karol
authors.initials: K
authors.lastname: Szymański
authors.affiliation: Intercollegiate Faculty of Biotechnology UG&MUG, Department of Molecular Bacteriology, Gdańsk, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Hinc
authors.affiliation: Intercollegiate Faculty of Biotechnology UG&MUG, Department of Molecular Bacteriology, Gdańsk, Poland.
authors.forename: Michał
authors.initials: M
authors.lastname: Obuchowski
authors.affiliation: Department of Microbiology, School of Natural Sciences, National University of Ireland Galway, Galway, Ireland.
authors.forename: Anthony P
authors.initials: AP
authors.lastname: Moran
authors.affiliation: Division of Gastroimmunology, Department of Immunology and Infectious Biology, Institute of Microbiology, Biotechnology and Immunology, Faculty of Biology and Environmental Protection, University of Lodz, Łódź, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Chmiela
mesh_terms: D000942:Antigens, Bacterial / Q000276:immunology*; D001241:Aspirin / Q000494:pharmacology*; D008078:Cholesterol, LDL / Q000502:physiology*; D005753:Gastric Mucosa / Q000187:drug effects / Q000276:immunology / Q000473:pathology*; D016480:Helicobacter pylori / Q000276:immunology*; D006801:Humans; D066298:In Vitro Techniques; D007653:Ketocholesterols / Q000502:physiology*
chemical_list: D000942:Antigens, Bacterial; D008078:Cholesterol, LDL; D007653:Ketocholesterols; C003001:7-ketocholesterol; D001241:Aspirin