title: Myeloperoxidase in human peripheral blood lymphocytes: Production and subcellular localization.
abstract: Myeloperoxidase (MPO) is an important enzyme in the front-line protection against microorganisms. In peripheral blood, it is accepted that MPO is only produced by myeloid-lineage cells. Thus, MPO presence is unexpected in lymphocytes. We showed recently that B1-lymphocytes from mice have MPO. Here, we showed that subsets of human peripheral B, CD4(+) and CD8(+) T lymphocytes express MPO. The content of MPO in lymphocytes was very low compared to neutrophils/monocytes with a preferential distribution in the nucleus and perinuclear region. Also, we performed a MPO mRNA expression analysis from human blood cells derived from microarray raw data publicly available, showing that MPO is modulated in infectious disease. MPO was increased in CD4(+) T lymphocytes from HIV chronic infection and in CD8(+) T lymphocytes from HCV-positive patients. Our study points out MPO as a multifunctional protein due to its subcellular localization and expression modulation in lymphocytes indicating alternative unknown functions for MPO in lymphocytes.
authors.affiliation: Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, São Paulo, SP 05508, Brazil.
authors.forename: Sabrina Sayori
authors.initials: SS
authors.lastname: Okada
authors.affiliation: Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, São Paulo, SP 05508, Brazil.
authors.forename: Edson Mendes
authors.initials: EM
authors.lastname: de Oliveira
authors.affiliation: Departamento de Ciências Biológicas, Universidade Federal de Alfenas, Alfenas, MG 37130, Brazil.
authors.forename: Tomaz Henrique
authors.initials: TH
authors.lastname: de Araújo
authors.affiliation: Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, Universidade Federal de Alfenas, Alfenas, MG 37130, Brazil.
authors.forename: Maria Rita
authors.initials: MR
authors.lastname: Rodrigues
authors.affiliation: Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, São Paulo, SP 05508, Brazil.
authors.forename: Renata Chaves
authors.initials: RC
authors.lastname: Albuquerque
authors.affiliation: Departamento de Microbiologia Imunobiologia e Parasitologia, Universidade Federal de São Paulo, São Paulo, SP 04023, Brazil.
authors.forename: Renato Arruda
authors.initials: RA
authors.lastname: Mortara
authors.affiliation: Núcleo de Microscopia Eletrônica, Instituto Adolfo Lutz, São Paulo, SP 01246, Brazil.
authors.forename: Noemi Nosomi
authors.initials: NN
authors.lastname: Taniwaki
authors.affiliation: Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, São Paulo, SP 05508, Brazil; Emory Vaccine Center, Yerkes National Primate Research Center, Emory University, Atlanta, GA 30329, USA.
authors.forename: Helder Imoto
authors.initials: HI
authors.lastname: Nakaya
authors.affiliation: Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, São Paulo, SP 05508, Brazil.
authors.forename: Ana
authors.initials: A
authors.lastname: Campa
authors.affiliation: Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, São Paulo, SP 05508, Brazil. Electronic address: carol@usp.br.
authors.forename: Ana Carolina Ramos
authors.initials: AC
authors.lastname: Moreno
mesh_terms: D001402:B-Lymphocytes / Q000201:enzymology* / Q000276:immunology; D015153:Blotting, Western; D015496:CD4-Positive T-Lymphocytes / Q000201:enzymology* / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000201:enzymology* / Q000276:immunology; D002469:Cell Separation; D005434:Flow Cytometry; D015658:HIV Infections / Q000201:enzymology / Q000276:immunology; D006526:Hepatitis C / Q000201:enzymology / Q000276:immunology; D006801:Humans; D016130:Immunophenotyping; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D020411:Oligonucleotide Array Sequence Analysis; D009195:Peroxidase / Q000096:biosynthesis* / Q000276:immunology; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D009195:Peroxidase
keywords: Electron microscopy; FACS; Fluorescence microscopy; Moonlighting protein; Subcellular localization