title: Selective separation and concentration of antihypertensive peptides from rapeseed protein hydrolysate by electrodialysis with ultrafiltration membranes.
abstract: Rapeseed protein isolate was subjected to alcalase digestion to obtain a protein hydrolysate that was separated into peptide fractions using electrodialysis with ultrafiltration membrane (EDUF) technology. The EDUF process (6h duration) led to isolation of three peptide fractions: anionic (recovered in KCl-1 compartment), cationic (recovered in KCl-2 compartment), and those that remained in the feed compartment, which was labeled final rapeseed protein hydrolysate (FRPH). As expected the KCl-1 peptides were enriched in negatively-charged (43.57%) while KCl-2 contained high contents of positively-charged (28.35%) amino acids. All the samples inhibited angiotensin converting enzyme (ACE) and renin activities in dose-dependent manner with original rapeseed protein hydrolysate having the least ACE-inhibitory IC50 value of 0.0932±0.0037 mg/mL while FRPH and KCl-2 had least renin-inhibitory IC50 values of 0.47±0.05 and 0.55±0.06 mg/mL, respectively. Six hours after oral administration (100 mg/kg body weight) to spontaneously hypertensive rats, the FRPH produced the maximum systolic blood pressure reduction of -51 mmHg.
authors.affiliation: College of Food Science & Engineering, Nanjing University of Finance and Economics, Nanjing 210023, People's Republic of China; Department of Human Nutritional Sciences and the Richardson Centre for Functional Foods and Nutraceuticals, University of Manitoba, Winnipeg, Manitoba R3T 2N2, Canada; College of Food Science, Jiangnan University, Wuxi 214122, People's Republic of China; College of Food Science and Engineering/Collaborative Innovation Center for Modern Grain Circulation and Safety/Key Laboratory of Grains and Oils Quality Control and Processing, Nanjing University of Finance and Economics, Nanjing 210023, China. Electronic address: rong.he@njue.edu.cn.
authors.forename: Rong
authors.initials: R
authors.lastname: He
authors.affiliation: Department of Human Nutritional Sciences and the Richardson Centre for Functional Foods and Nutraceuticals, University of Manitoba, Winnipeg, Manitoba R3T 2N2, Canada.
authors.forename: Abraham T
authors.initials: AT
authors.lastname: Girgih
authors.affiliation: Department of Food Sciences, Laval University, Quebec G1V 0A6, Canada.
authors.forename: Elodie
authors.initials: E
authors.lastname: Rozoy
authors.affiliation: Department of Food Sciences, Laval University, Quebec G1V 0A6, Canada.
authors.forename: Laurent
authors.initials: L
authors.lastname: Bazinet
authors.affiliation: College of Food Science & Engineering, Nanjing University of Finance and Economics, Nanjing 210023, People's Republic of China; College of Food Science, Jiangnan University, Wuxi 214122, People's Republic of China.
authors.forename: Xing-Rong
authors.initials: XR
authors.lastname: Ju
authors.affiliation: Department of Human Nutritional Sciences and the Richardson Centre for Functional Foods and Nutraceuticals, University of Manitoba, Winnipeg, Manitoba R3T 2N2, Canada. Electronic address: rotimi.aluko@umanitoba.ca.
authors.forename: Rotimi E
authors.initials: RE
authors.lastname: Aluko
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors / Q000032:analysis / Q000737:chemistry; D000818:Animals; D000959:Antihypertensive Agents / Q000032:analysis*; D001794:Blood Pressure / Q000187:drug effects; D029741:Brassica rapa / Q000737:chemistry*; D003956:Dialysis; D008297:Male; D010455:Peptides / Q000032:analysis*; D007703:Peptidyl-Dipeptidase A / Q000494:pharmacology; D011492:Protein Hydrolysates / Q000032:analysis*; D051381:Rats; D011918:Rats, Inbred SHR; D014462:Ultrafiltration; D000092502:Renin Inhibitors
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D000959:Antihypertensive Agents; D010455:Peptides; D007703:Peptidyl-Dipeptidase A; D011492:Protein Hydrolysates; D000092502:Renin Inhibitors; C488034:ACE protein, human
keywords: ACE; Electrodialysis with ultrafiltration membrane; Protein hydrolysate; Rapeseed; Renin; Spontaneously hypertensive rats; Systolic blood pressure