title: Characterization, antioxidative and bifidogenic effects of polysaccharides from Pleurotus eryngii after heat treatments.
abstract: Polysaccharides were extracted from freeze-dried-, oven-dried- and boiling-treated Pleurotus eryngii (FDPEPS, ODPEPS and BTPEPS). Yield of FDPEPS and its total carbohydrates, total polysaccharides, reducing sugars, phenolics and protein content were higher than ODPEPS and BTPEPS. High performance liquid chromatography (HPLC) identified that FDPEPS, ODPEPS, and BTPEPS were composed of mannose (5.75%, 5.52%, 6.97%), glucose (88.90%, 89.31%, 87.68%) and galactose (5.34%, 5.17%, 5.35%). All three polysaccharides showed typical saccharic absorption bands in FT-IR. The FDPEPS showed the highest antioxidant activities in ferric reducing antioxidant power (FRAP), ABTS, superoxide anion and hydroxyl radical scavenging tests. Denser aggregates and larger serum pores were observed in confocal micrographs of soymilk added with ODPEPS. Viability of Bifidobacterium longum in soymilk added with polysaccharides was significantly higher (p<0.01) than those without polysaccharides during fermentation. Heat treatments applied before extraction affected the properties, composition and microstructures of FDPEPS, ODPEPS and BTPEPS.
authors.affiliation: Food and Nutritional Science, School of Biological Sciences, The University of Hong Kong, Pokfulam Road, Hong Kong. Electronic address: siqianli@hku.hk.
authors.forename: Siqian
authors.initials: S
authors.lastname: Li
authors.affiliation: Food and Nutritional Science, School of Biological Sciences, The University of Hong Kong, Pokfulam Road, Hong Kong. Electronic address: npshah@hku.hk.
authors.forename: Nagendra P
authors.initials: NP
authors.lastname: Shah
mesh_terms: D000975:Antioxidants / Q000032:analysis* / Q000494:pharmacology; D001644:Bifidobacterium / Q000187:drug effects / Q000254:growth & development*; D002851:Chromatography, High Pressure Liquid; D005285:Fermentation; D005612:Freeze Drying; D062610:Fungal Polysaccharides / Q000032:analysis* / Q000494:pharmacology; D005690:Galactose / Q000032:analysis; D005947:Glucose / Q000032:analysis; D006358:Hot Temperature; D017665:Hydroxyl Radical / Q000737:chemistry; D008358:Mannose / Q000032:analysis; D010084:Oxidation-Reduction; D010636:Phenols / Q000032:analysis; D020076:Pleurotus / Q000737:chemistry*; D041942:Soy Milk / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D013481:Superoxides / Q000737:chemistry
chemical_list: D000975:Antioxidants; D062610:Fungal Polysaccharides; D010636:Phenols; D013481:Superoxides; D017665:Hydroxyl Radical; D005947:Glucose; D008358:Mannose; D005690:Galactose
keywords: Antioxidant activity; Bifidogenic effect; Heat treatment; Pleurotus eryngii; Polysaccharides; Structure characterization