title: A standardized kit for automated quantitative assessment of candidate protein biomarkers in human plasma.
abstract: BACKGROUND
  An increasingly popular mass spectrometry-based quantitative approach for health-related research in the biomedical field involves the use of stable isotope-labeled standards (SIS) and multiple/selected reaction monitoring (MRM/SRM). To improve inter-laboratory precision and enable more widespread use of this 'absolute' quantitative technique in disease-biomarker assessment studies, methods must be standardized. Results/methodology: Using this MRM-with-SIS-peptide approach, we developed an automated method (encompassing sample preparation, processing and analysis) for quantifying 76 candidate protein markers (spanning >4 orders of magnitude in concentration) in neat human plasma.
  
  
  CONCLUSIONS
  The assembled biomarker assessment kit - the 'BAK-76' - contains the essential materials (SIS mixes), methods (for acquisition and analysis), and tools (Qualis-SIS software) for performing biomarker discovery or verification studies in a rapid and standardized manner.
authors.affiliation: University of Victoria - Genome British Columbia Proteomics Centre, Vancouver Island Technology Park, #3101-4464 Markham St., Victoria, BC V8Z 7X8, Canada.
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Percy
authors.affiliation: University of Victoria - Genome British Columbia Proteomics Centre, Vancouver Island Technology Park, #3101-4464 Markham St., Victoria, BC V8Z 7X8, Canada.|Center for Proteomics & Metabolomics, Leiden University Medical Center, 2333 ZA, Leiden, The Netherlands.
authors.forename: Yassene
authors.initials: Y
authors.lastname: Mohammed
authors.affiliation: University of Victoria - Genome British Columbia Proteomics Centre, Vancouver Island Technology Park, #3101-4464 Markham St., Victoria, BC V8Z 7X8, Canada.
authors.forename: Juncong
authors.initials: J
authors.lastname: Yang
authors.affiliation: University of Victoria - Genome British Columbia Proteomics Centre, Vancouver Island Technology Park, #3101-4464 Markham St., Victoria, BC V8Z 7X8, Canada.|Department of Biochemistry & Microbiology, University of Victoria, Petch Building Room 207, 3800 Finnerty Rd., Victoria, BC V8P 5C2, Canada.
authors.forename: Christoph H
authors.initials: CH
authors.lastname: Borchers
mesh_terms: D015415:Biomarkers / Q000097:blood*; D001798:Blood Proteins / Q000032:analysis*; D006801:Humans; D011786:Quality Control; D012015:Reference Standards
chemical_list: D015415:Biomarkers; D001798:Blood Proteins