title: C-terminal domains of a histone demethylase interact with a pair of transcription factors and mediate specific chromatin association.
abstract: JmjC domain containing protein 14 (JMJ14) is an H3K4-specific histone demethylase that plays important roles in RNA-mediated gene silencing and flowering time regulation inArabidopsis. However, how JMJ14 is recruited to its target genes remains unclear. Here, we show that the C-terminal FYRN and FYRC domains of JMJ14 are required for RNA silencing and flowering time regulation. Chromatin binding of JMJ14 is lost upon deletion of its FYRN and FYRC domains, and H3K4me3 is increased. FYRN and FYRC domains interact with a pair of NAC domain containing transcription factors, NAC050 and NAC052. Genome-wide ChIP analysis revealed that JMJ14 and NAC050/052 share a set of common target genes with CTTGNNNNNCAAG consensus sequences. Mutations in eitherNAC052orNAC050impair RNA-mediated gene silencing. Together, our findings demonstrate an important role of FYRN and FYRC domains in targeting JMJ14 through direct interaction with NAC050/052 proteins, which reveals a novel mechanism of histone demethylase recruitment.
authors.affiliation: State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China ; College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Shuaibin
authors.initials: S
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China ; College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Yanyuan
authors.initials: Y
authors.lastname: Kang
authors.affiliation: State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Cui
authors.affiliation: Department of Molecular, Cell, and Developmental Biology, University of California, Los Angeles, CA 90095.
authors.forename: Ao
authors.initials: A
authors.lastname: Liu
authors.affiliation: Department of Molecular, Cell, and Developmental Biology, University of California, Los Angeles, CA 90095.
authors.forename: Angelique
authors.initials: A
authors.lastname: Deleris
authors.affiliation: Department of Molecular, Cell, and Developmental Biology, University of California, Los Angeles, CA 90095.
authors.forename: Maxim V C
authors.initials: MV
authors.lastname: Greenberg
authors.affiliation: State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Xiekui
authors.initials: X
authors.lastname: Cui
authors.affiliation: State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China ; College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Qiu
authors.affiliation: State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Falong
authors.initials: F
authors.lastname: Lu
authors.affiliation: Department of Biological Chemistry, University of California, Los Angeles, CA 90095.
authors.forename: James A
authors.initials: JA
authors.lastname: Wohlschlegel
authors.affiliation: Department of Molecular, Cell, and Developmental Biology, University of California, Los Angeles, CA 90095 ; Howard Hughes Medical Institute, University of California, Los Angeles, CA 90095.
authors.forename: Steven E
authors.initials: SE
authors.lastname: Jacobsen
authors.affiliation: State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China ; Collaborative Innovation Center of Genetics and Development, Fudan University, Shanghai 200433, China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Cao
keywords: JMJ14; NAC050; NAC052; histone demethylase; transgene silencing