title: Overexpression of Prdx1 in hilar cholangiocarcinoma: a predictor for recurrence and prognosis.
abstract: Prdx1 is an important member of peroxiredoxins (Prdxs) regulating various cellular signaling and differentiation. Prdx1 confers an aggressive survival phenotype of cancer cells and drug-resistance, yet its role in hilar cholangiocarcinoma is not fully investigated. In present study, we detected the expression profile of Prdx1 in 88 hilar cholangiocarcinoma by tissue arrays and immunohistochemistry. Prdx1 level was down-regulated by specific Prdx1-shRNA in vitro and the possible mechanism was investigated. Overexpression of Prdx1 was observed in 53 of 88 cases (60.2%). Prdx1 expression was significantly associated with tumor invasion, nodal metastasis, advanced disease stage. Down-regulation of Prdx1 inhibited cell proliferation and colony formation of QBC939 cells and reduced the level of SNAT1 expression. Patients with Prdx1 overexpression had a shorter disease-free survival and overall survival than those without Prdx1 expression. Multivariate analysis showed that Prdx1 was an independent prognostic factor for patients with hilar cholangiocarcinoma. The data indicate that Prdx1 may contribute to the development and progression of hilar cholangiocarcinoma, partially through regulating SNAT1 expression, and may be used as a biomarker in predicting the outcome of patients with hilar cholangiocarcinoma.
authors.affiliation: Department of Radiology, Shanghai East Hospital, Tongji University School of Medicine Shanghai 200120, China ; Department of Radiology, Changzheng Hospital Shanghai 20003, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Department of Radiology, Shanghai East Hospital, Tongji University School of Medicine Shanghai 200120, China.
authors.forename: Weiwen
authors.initials: W
authors.lastname: Shen
authors.affiliation: Department of Oncology, Changzheng Hospital Shanghai 200433, China.
authors.forename: Xiaojing
authors.initials: X
authors.lastname: He
authors.affiliation: Department of Radiology, Shanghai East Hospital, Tongji University School of Medicine Shanghai 200120, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Qian
authors.affiliation: Department of Radiology, Changzheng Hospital Shanghai 20003, China.
authors.forename: Shiyuan
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Medical Oncology, Shanghai East Hospital, Tongji University School of Medicine Shanghai 200120, China.
authors.forename: Guanzhen
authors.initials: G
authors.lastname: Yu
mesh_terms: D000328:Adult; D000368:Aged; D001650:Bile Duct Neoplasms / Q000378:metabolism / Q000401:mortality / Q000473:pathology*; D014408:Biomarkers, Tumor / Q000032:analysis*; D015153:Blotting, Western; D018572:Disease-Free Survival; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D018285:Klatskin Tumor / Q000378:metabolism / Q000401:mortality / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local / Q000378:metabolism / Q000473:pathology; D054464:Peroxiredoxins / Q000096:biosynthesis*; D011379:Prognosis; D016016:Proportional Hazards Models; D034741:RNA, Small Interfering; D046888:Tissue Array Analysis; D014162:Transfection; D015854:Up-Regulation
chemical_list: D014408:Biomarkers, Tumor; D034741:RNA, Small Interfering; C515940:PRDX1 protein, human; D054464:Peroxiredoxins
keywords: Hilar cholangiocarcinoma; Prdx1; SNAT1; prognosis; tissue microarray