title: Microarray Developed on Plastic Substrates.
abstract: There is a huge potential interest to use synthetic polymers as versatile solid supports for analytical microarraying. Chemical modification of polycarbonate (PC) for covalent immobilization of probes, micro-printing of protein or nucleic acid probes, development of indirect immunoassay, and development of hybridization protocols are described and discussed.
authors.affiliation: Departamento de Química, Instituto interuniversitario de Reconocimiento Molecular y Desarrollo Tecnológico, Universitat Politècnica de València, Camino de Vera s/n, 46022, Valencia, Spain.
authors.forename: María-José
authors.initials: MJ
authors.lastname: Bañuls
authors.affiliation: Departamento de Química, Instituto interuniversitario de Reconocimiento Molecular y Desarrollo Tecnológico, Universitat Politècnica de València, Camino de Vera s/n, 46022, Valencia, Spain.
authors.forename: Sergi B
authors.initials: SB
authors.lastname: Morais
authors.affiliation: Departamento de Química, Instituto interuniversitario de Reconocimiento Molecular y Desarrollo Tecnológico, Universitat Politècnica de València, Camino de Vera s/n, 46022, Valencia, Spain.
authors.forename: Luis A
authors.initials: LA
authors.lastname: Tortajada-Genaro
authors.affiliation: Departamento de Química, Instituto interuniversitario de Reconocimiento Molecular y Desarrollo Tecnológico, Universitat Politècnica de València, Camino de Vera s/n, 46022, Valencia, Spain. amaquieira@qim.upv.es.
authors.forename: Ángel
authors.initials: Á
authors.lastname: Maquieira
mesh_terms: D000447:Aldehydes / Q000737:chemistry; D000588:Amines / Q000737:chemistry; D001419:Bacteria / Q000302:isolation & purification; D002264:Carboxylic Acids / Q000737:chemistry; D006241:Haptens / Q000737:chemistry; D007118:Immunoassay; D046228:Microarray Analysis / Q000379:methods*; D009693:Nucleic Acid Hybridization; D010969:Plastics / Q000737:chemistry*; D011076:Polycarboxylate Cement / Q000737:chemistry; D011506:Proteins / Q000737:chemistry; D013499:Surface Properties
chemical_list: D000447:Aldehydes; D000588:Amines; D002264:Carboxylic Acids; D006241:Haptens; D010969:Plastics; D011076:Polycarboxylate Cement; D011506:Proteins; C005506:polycarbonate
keywords: Hybridization assay; Immunoassay; Optical detection; Plastic substrates; Polycarbonate; Probe covalent immobilization; Surface functionalization; Synthetic polymers