title: Single Amino Acid Switch between a Flavin-Dependent Dehalogenase and Nitroreductase.
abstract: A single mutation within a flavoprotein is capable of switching the catalytic activity of a dehalogenase into a nitroreductase. This change in function correlates with a destabilization of the one-electron-reduced flavin semiquinone that is differentially expressed in the nitro-FMN reductase superfamily during redox cycling. The diversity of function within such a superfamily therefore has the potential to arise from rapid evolution, and its members should provide a convenient basis for developing new catalysts with an altered specificity of choice.
authors.affiliation: Department of Chemistry, Johns Hopkins University , Baltimore, Maryland 21218, United States.
authors.forename: Arnab
authors.initials: A
authors.lastname: Mukherjee
authors.affiliation: Department of Chemistry, Johns Hopkins University , Baltimore, Maryland 21218, United States.
authors.forename: Steven E
authors.initials: SE
authors.lastname: Rokita
mesh_terms: D000596:Amino Acids / Q000737:chemistry* / Q000235:genetics; D005415:Flavins / Q000737:chemistry* / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D015394:Molecular Structure; D009154:Mutation; D009601:Nitroreductases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D010088:Oxidoreductases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
chemical_list: D000596:Amino Acids; D005415:Flavins; D010088:Oxidoreductases; D009601:Nitroreductases