title: Transmembrane 7 superfamily member 4 regulates cell cycle progression in breast cancer cells.
abstract: OBJECTIVE
  TM7SF4 (transmembrane 7 superfamily member 4) gene encodes a seven-pass transmembrane protein that is primarily expressed in dendritic cells called as dendritic cell-specific expressed seven transmembrane protein (DC-STAMP). This protein regulates immunological functions, osteoclastogenesis and myeloid differentiation. Although the roles of TM7SF4 have been currently studied on Paget's disease of bone and papillary thyroid cancers, it is unclear whether TM7SF4 plays a role in breast cancer. In current study, we investigated the expression of TM7SF4 in human breast cancer cell lines.
  
  
  METHODS
  In this study, five breast cancer lines were cultured. Small hairpin RNA against TM7SF4 using a lentiviral vector was generated and transfected into MCF-7 breast cancer cells. Effects of down-regulating TM7SF4 in transfected cells were examined by Western blot, RT-PCR, apoptotic rate, colony formation, and cell cycle analyses.
  
  
  RESULTS
  The results demonstrated that down-regulation of TM7SF4 led to a decrease in colony formation in MCF-7 cells compared to the control group. This is likely due to a decrease in proliferation and cell cycle and an increase in apoptosis.
  
  
  CONCLUSIONS
  To our knowledge, our data demonstrate for the first time that TM7SF4 plays an essential role in regulating cell cycle progression in breast cancer.
authors.affiliation: Department of Pathology, The Third Affiliated Hospital, Zhengzhou University, Zhengzhou, People Republic of China. xianxu77@163.com.
authors.forename: X X
authors.initials: XX
authors.lastname: Zeng
authors.forename: T J
authors.initials: TJ
authors.lastname: Chu
authors.forename: J Y
authors.initials: JY
authors.lastname: Yuan
authors.forename: W
authors.initials: W
authors.lastname: Zhang
authors.forename: Y M
authors.initials: YM
authors.lastname: Du
authors.forename: Z Y
authors.initials: ZY
authors.lastname: Ban
authors.forename: D M
authors.initials: DM
authors.lastname: Lei
authors.forename: J
authors.initials: J
authors.lastname: Cao
authors.forename: Z
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000502:physiology*; D017209:Apoptosis / Q000502:physiology; D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology; D002453:Cell Cycle / Q000502:physiology*; D045744:Cell Line, Tumor; D018450:Disease Progression; D005260:Female; D006801:Humans; D061986:MCF-7 Cells; D008565:Membrane Proteins / Q000502:physiology*; D034741:RNA, Small Interfering / Q000494:pharmacology / Q000502:physiology
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C501589:DCSTAMP protein, human; D008565:Membrane Proteins; D034741:RNA, Small Interfering