title: Actinorectispora indicagen. nov., sp. nov. isolated from soil, a member of the familyPseudonocardiaceae.
abstract: The taxonomic positions of three Gram-stain-positive, aerobic strains, designated YIM 75722, 75726 and 75728T, and isolated from a soil sample collected from Kurnool of Andhra Pradesh province, India, were assessed using a polyphasic approach. Growth was observed at pH 7.0-10.0 (optimum pH 7.0), 15-28 °C (optimum 28 °C) and 0-8 % (w/v) NaCl (grew without NaCl). Strains showed cylindrical spores with straight-chain morphology on aerial mycelium, but did not reveal sporangium-like structures or fragmentation of the substrate mycelium. Whole-cell hydrolysates of all strains contained galactose and ribose as the diagnostic sugars andmeso-diaminopimelic acid as the diamino acid. The predominant menaquinone was MK-9(H4); MK-9 (H6) and MK-10 (H4) were present in smaller amounts. The phospholipid pattern consisted mainly of diphosphatidylglycerol, phosphatidylglycerol and phosphatidylcholine. The major fatty acids were i-C15 : 0, ai-C15 : 0, i-C17 : 0and ai-C17 : 0. The genomic DNA G+C content was 68.0 mol%. Phylogenetic analysis, based on 16S rRNA gene sequences, revealed that strain YIM 75728Tshould be placed within the familyPseudonocardiaceae, in which the strain formed a distinct lineage. The combination of phylogenetic analysis, phenotypic characteristics and chemotaxonomic data support the conclusion that strain YIM 75728Trepresents a novel species of a novel genus of the familyPseudonocardiaceaefor which the nameActinorectispora indicagen. nov., sp. nov., is proposed. Strain YIM 75728T( = DSM 45410T= CCTCC AA 209065T) is the type strain ofActinorectispora indica. Strain YIM 75728Twas considered as the type strain over the other two strains based on the highest sequence read length of the strain.
authors.affiliation: Department of Medical Laboratory Technology,Faculty of Applied Medical Sciences, Northern Border University, Arar-91431, Northern Borders,Kingdom of Saudi Arabia.|Yunnan Institute of Microbiology,Yunnan University, Kunming- 650091, Yunnan, PRChina.|Department of Microbiology,Gulbarga University, Gulbarga- 585106, Karnataka,India.
authors.forename: Syed Raziuddin
authors.initials: SR
authors.lastname: Quadri
authors.affiliation: Key Laboratory of Marine Bio-resources Sustainable Utilization CAS,RNAM Center for Marine Microbiology, Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou- 510301, PRChina.
authors.forename: Xin-Peng
authors.initials: XP
authors.lastname: Tian
authors.affiliation: Key Laboratory of Marine Bio-resources Sustainable Utilization CAS,RNAM Center for Marine Microbiology, Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou- 510301, PRChina.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Medical Laboratory Technology,Faculty of Applied Medical Sciences, Northern Border University, Arar-91431, Northern Borders,Kingdom of Saudi Arabia.
authors.forename: Jamal Al
authors.initials: JA
authors.lastname: Ruwaili
authors.affiliation: Bioproducts Research Chair (BRC),College of Science, King Saud University, Riyadh 11451,Kingdom of Saudi Arabia.
authors.forename: Wael N
authors.initials: WN
authors.lastname: Hozzein
authors.affiliation: Department of Microbiology,Gulbarga University, Gulbarga- 585106, Karnataka,India.
authors.forename: Dayanand
authors.initials: D
authors.lastname: Agsar
authors.affiliation: Yunnan Institute of Microbiology,Yunnan University, Kunming- 650091, Yunnan, PRChina.|State Key Laboratory of Biocontrol,Key Laboratory of Biodiversity Dynamics and Conservation of Guangdong Higher Education Institutes, College of Ecology and Evolution, Sun Yat-Sen University, Guangzhou 510275, PRChina.
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Li
authors.affiliation: NCIM-Resource Center,CSIR-National Chemical Laboratory, Pune- 411008,India.
authors.forename: Syed G
authors.initials: SG
authors.lastname: Dastager