title: An integrated genomic and proteomic approach to identify signatures of endosulfan exposure in hepatocellular carcinoma cells.
abstract: Present study reports the identification of genomic and proteomic signatures of endosulfan exposure in hepatocellular carcinoma cells (HepG2). HepG2 cells were exposed to sublethal concentration (15μM) of endosulfan for 24h. DNA microarray and MALDI-TOF-MS analyses revealed that endosulfan induced significant alterations in the expression level of genes and proteins involved in multiple cellular pathways (apoptosis, transcription, immune/inflammatory response, carbohydrate metabolism, etc.). Furthermore, downregulation of PHLDA gene, upregulation of ACIN1 protein and caspase-3 activation in exposed cells indicated that endosulfan can trigger apoptotic cascade in hepatocellular carcinoma cells. In total 135 transcripts and 19 proteins were differentially expressed. This study presents an integrated approach to identify the alteration of biological/cellular pathways in HepG2 cells upon endosulfan exposure.
authors.affiliation: Environmental Health Division, CSIR-National Environmental Engineering Research Institute (NEERI), Nagpur, India.
authors.forename: Deepa
authors.initials: D
authors.lastname: Gandhi
authors.affiliation: Environmental Health Division, CSIR-National Environmental Engineering Research Institute (NEERI), Nagpur, India.
authors.forename: Prashant
authors.initials: P
authors.lastname: Tarale
authors.affiliation: Environmental Health Division, CSIR-National Environmental Engineering Research Institute (NEERI), Nagpur, India.
authors.forename: Pravin K
authors.initials: PK
authors.lastname: Naoghare
authors.affiliation: Environmental Health Division, CSIR-National Environmental Engineering Research Institute (NEERI), Nagpur, India.
authors.forename: Amit
authors.initials: A
authors.lastname: Bafana
authors.affiliation: Environmental Health Division, CSIR-National Environmental Engineering Research Institute (NEERI), Nagpur, India.
authors.forename: Kannan
authors.initials: K
authors.lastname: Krishnamurthi
authors.affiliation: CNR Institute for Macromolecular Studies, Genoa, Italy.
authors.forename: Patrizio
authors.initials: P
authors.lastname: Arrigo
authors.affiliation: Environmental Health Division, CSIR-National Environmental Engineering Research Institute (NEERI), Nagpur, India. Electronic address: ss_devi@neeri.res.in.
authors.forename: Sivanesan
authors.initials: S
authors.lastname: Saravanadevi
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D004726:Endosulfan / Q000633:toxicity*; D020869:Gene Expression Profiling; D023281:Genomics / Q000379:methods*; D056945:Hep G2 Cells; D006801:Humans; D007306:Insecticides / Q000633:toxicity*; D008113:Liver Neoplasms / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D011506:Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D040901:Proteomics / Q000379:methods*
chemical_list: D007306:Insecticides; D011506:Proteins; D004726:Endosulfan
keywords: Common signatures; Endosulfan; Gene microarray; Hepatocellular carcinoma cells (HepG2); Proteomics