title: In vitro anticancer activities of Schiff base and its lanthanum complex.
abstract: Schiff base metal complexes are well-known to intercalate DNA. The La(III) complexes have been synthesized such that they hinder with the role of the topoisomerases, which control the topology of DNA during the cell-division cycle. Although several promising chemotherapeutics have been developed, on the basis of Schiff base metal complex DNA intercalating system they did not proceed past clinical trials due to their dose-limiting toxicity. Herein, we discuss an alternative compound, the La(III) complex, [La(L(1))2Cl3]·7H2O based on a Schiff base ligand 2,3-dihydro-1H-indolo-[2,3-b]-phenazin-4(5H)-ylidene)benzothiazole-2-amine (L(1)), and report in vitro cell studies. Results of antitumor activity using cell viability assay, reactive oxygen species (ROS) generation and nuclear condensation in PC-3 (Human, prostate carcinoma) cells show that the metal complex is more potent than ligand. La(III) complexes have been synthesized by reaction of lanthanum(III) salt in 1:2M ratio with ligands L(1) and 3-(ethoxymethylene)-2,3-dihydro-1H-indolo[2,3-b]-phenazin-4(5H)-ylidene)benzathiazole-2-amine (L(2)) in methanol. The ligands and their La(III) complexes were characterized by molar conductance, magnetic susceptibility, elemental analyses, FT-IR, UV-Vis, (1)H/(13)C NMR, thermogravimetric, XRD, and SEM analysis.
authors.affiliation: Department of Chemistry, Banasthali University, Rajasthan 304022, India.
authors.lastname: Neelima
authors.affiliation: Department of Chemistry, Banasthali University, Rajasthan 304022, India.
authors.forename: Kavita
authors.initials: K
authors.lastname: Poonia
authors.affiliation: Molecular Endocrinology Lab, Department of Zoology, Lucknow University, UP, India.
authors.forename: Sahabjada
authors.initials: S
authors.lastname: Siddiqui
authors.affiliation: Molecular Endocrinology Lab, Department of Zoology, Lucknow University, UP, India.
authors.forename: Md
authors.initials: M
authors.lastname: Arshad
authors.affiliation: Department of Chemistry, Banasthali University, Rajasthan 304022, India. Electronic address: dsbchoudhary2002@gmail.com.
authors.forename: Dinesh
authors.initials: D
authors.lastname: Kumar
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology; D052160:Benzothiazoles / Q000737:chemistry / Q000494:pharmacology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D056831:Coordination Complexes / Q000737:chemistry* / Q000494:pharmacology; D006801:Humans; D007811:Lanthanum / Q000737:chemistry* / Q000494:pharmacology; D008297:Male; D008958:Models, Molecular; D011467:Prostate / Q000187:drug effects; D011471:Prostatic Neoplasms / Q000188:drug therapy; D012545:Schiff Bases / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D000970:Antineoplastic Agents; D052160:Benzothiazoles; D056831:Coordination Complexes; D012545:Schiff Bases; C523775:aminobenzothiazole compound; D007811:Lanthanum
keywords: In-vitro anticancer activities; Lanthanum complexes; Schiff base