title: Periodontal disease exacerbates systemic ovariectomy-induced bone loss in mice.
abstract: Periodontal pathogens and/or inflammatory products from periodontitis participate in the development or progression of systemic diseases. In this context, periodontitis acts as a modifying factor to systemic health, including diabetes and cardiovascular diseases. Osteoporosis is an increasingly prevalent condition in our aging population and considered a risk factor for periodontal disease, but the effect of periodontitis on systemic bone homeostasis is unknown. We thus evaluated the effects of experimental periodontitis (EP) on systemic bone loss and the influence of estrogen deficiency in this context, using a mouse model of combined periodontitis and osteoporosis. Experimental periodontitis (EP) was induced by a ligature insertion around the mandibular first molars and Porphyromonas gingivalis infection. Three-dimensional microcomputed tomographic analyses performed 48days following infection revealed that EP and ovariectomy (OVX) induced a significantly higher femoral and mandibular bone loss compared to EP or OVX alone. EP alone did not induce systemic bone loss. In addition, the EP+OVX and EP groups showed significantly higher levels of tumor necrosis factor (TNF)-α than OVX and control groups at end point. These results suggest that periodontitis could be a risk factor for systemic bone loss, especially in post-menopausal women, and warrant further clinical investigations to confirm this association and propose adapted prophylactic and curative therapies.
authors.affiliation: Department of Bioscience and Oral Diagnosis, Institute of Science and Technology of São José dos Campos, Univ Estadual Paulista-UNESP, São José dos Campos, São Paulo, Brazil. Electronic address: ana.anbinder@fosjc.unesp.br.
authors.forename: Ana Lia
authors.initials: AL
authors.lastname: Anbinder
authors.affiliation: Department of Bioscience and Oral Diagnosis, Institute of Science and Technology of São José dos Campos, Univ Estadual Paulista-UNESP, São José dos Campos, São Paulo, Brazil.
authors.forename: Renata M
authors.initials: RM
authors.lastname: Moraes
authors.affiliation: Department of Bioscience and Oral Diagnosis, Institute of Science and Technology of São José dos Campos, Univ Estadual Paulista-UNESP, São José dos Campos, São Paulo, Brazil.
authors.forename: Gabriela M G
authors.initials: GMG
authors.lastname: Lima
authors.affiliation: Department of Bioscience and Oral Diagnosis, Institute of Science and Technology of São José dos Campos, Univ Estadual Paulista-UNESP, São José dos Campos, São Paulo, Brazil.
authors.forename: Felipe E
authors.initials: FE
authors.lastname: Oliveira
authors.affiliation: Department of Bioscience and Oral Diagnosis, Institute of Science and Technology of São José dos Campos, Univ Estadual Paulista-UNESP, São José dos Campos, São Paulo, Brazil.
authors.forename: Débora R C
authors.initials: DRC
authors.lastname: Campos
authors.affiliation: Department of Bioscience and Oral Diagnosis, Institute of Science and Technology of São José dos Campos, Univ Estadual Paulista-UNESP, São José dos Campos, São Paulo, Brazil.
authors.forename: Rodnei D
authors.initials: RD
authors.lastname: Rossoni
authors.affiliation: Department of Bioscience and Oral Diagnosis, Institute of Science and Technology of São José dos Campos, Univ Estadual Paulista-UNESP, São José dos Campos, São Paulo, Brazil.
authors.forename: Luciane D
authors.initials: LD
authors.lastname: Oliveira
authors.affiliation: Department of Bioscience and Oral Diagnosis, Institute of Science and Technology of São José dos Campos, Univ Estadual Paulista-UNESP, São José dos Campos, São Paulo, Brazil.
authors.forename: Juliana C
authors.initials: JC
authors.lastname: Junqueira
authors.affiliation: Vanderbilt Center for Bone Biology, Department of Medicine, Department of Pharmacology and Department of Cancer Biology, Vanderbilt University Medical Center, Nashville, TN 37215, USA.
authors.forename: Yun
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Vanderbilt Center for Bone Biology, Department of Medicine, Department of Pharmacology and Department of Cancer Biology, Vanderbilt University Medical Center, Nashville, TN 37215, USA.
authors.forename: Florent
authors.initials: F
authors.lastname: Elefteriou
mesh_terms: D000818:Animals; D001835:Body Weight; D001862:Bone Resorption / Q000209:etiology* / Q000473:pathology*; D024042:Collagen Type I / Q000097:blood / Q000652:urine; D016207:Cytokines / Q000097:blood / Q000652:urine; D003739:Dental Cementum / Q000473:pathology; D003743:Dental Enamel / Q000473:pathology; D018450:Disease Progression; D005260:Female; D005269:Femur / Q000000981:diagnostic imaging / Q000473:pathology; D008334:Mandible / Q000000981:diagnostic imaging / Q000473:pathology; D008807:Mice, Inbred BALB C; D015675:Osteocalcin / Q000097:blood / Q000652:urine; D010052:Ovariectomy / Q000009:adverse effects*; D010455:Peptides / Q000097:blood / Q000652:urine; D010510:Periodontal Diseases / Q000150:complications* / Q000473:pathology*; D014409:Tumor Necrosis Factor-alpha / Q000097:blood / Q000652:urine; D055114:X-Ray Microtomography
chemical_list: D024042:Collagen Type I; D016207:Cytokines; D010455:Peptides; D014409:Tumor Necrosis Factor-alpha; C052929:collagen type I trimeric cross-linked peptide; D015675:Osteocalcin
keywords: Fracture risk; Osteoporosis; Ovariectomy; Periodontitis; TNF-α