title: FSH/LH ratio in females and intracytoplasmic sperm injection.
abstract: OBJECTIVE
  To observe the effect of follicle stimulating hormone-luteinizing hormone ratio on oocyte parameters, quality of embryo, implantation and clinical pregnancy rate after intracytoplasmic sperm injection.
  
  
  METHODS
  The retrospective study was conducted at a fertility clinic in Islamabad, Pakistan, and comprised data of primary infertile females who underwent intracytoplasmic sperm injection from June 2011 to March 2013. All subjects had duration of infertility more than two years, and age range was 20-40 years. Follicle stimulating hormone and luteinizing hormone were estimated by enzyme-linked immunosorbent assay on day 3 of the cycle and the ratio was calculated. Groups were stratified on the basis of median ratio into groups I <1.26 and group II >1.26. SPSS 20 was used for data analysis.
  
  
  RESULTS
  Of the 282 females, 143(51%) were in group I and 139(49%) in group II. Pregnancy was acquired by 79(55%) and 22(16%) females in group I and II respectively. The number of retrieved, metaphase, fertilised oocytes, cleaved embryos and endometrial thickness was significantly larger in group I (p< 0.0001).
  
  
  CONCLUSIONS
  Follicle stimulating hormone-luteinizing hormone ratio less than 1.26 was associated with good oocyte parameters, top quality embryo and implantation after intracytoplasmic sperm injection.
authors.affiliation: Department of Biological and Biomedical Sciences, Aga Khan University, Karachi, Pakistan.
authors.forename: Rehana
authors.initials: R
authors.lastname: Rehman
authors.affiliation: 2nd Year Student, Dow Medical College, Karachi, Pakistan.
authors.forename: Hareem
authors.initials: H
authors.lastname: Syed
authors.affiliation: Department of Peadriatics, Biological & Biomedical Sciences, Aga Khan University, Karachi, Pakistan.
authors.forename: Najeeha Talat
authors.initials: NT
authors.lastname: Iqbal
authors.affiliation: Final Year Student, Dow Medical College, Karachi, Pakistan.
authors.forename: Sara
authors.initials: S
authors.lastname: Arif
authors.affiliation: Department of Biological and Biomedical Sciences, Aga Khan University, Karachi, Pakistan.
authors.forename: Saeeda
authors.initials: S
authors.lastname: Sheharyar
mesh_terms: D000328:Adult; D010064:Embryo Implantation; D005260:Female; D005640:Follicle Stimulating Hormone / Q000097:blood*; D006801:Humans; D007986:Luteinizing Hormone / Q000097:blood*; D010154:Pakistan; D011247:Pregnancy; D011256:Pregnancy Outcome; D012189:Retrospective Studies; D020554:Sperm Injections, Intracytoplasmic; D055815:Young Adult
chemical_list: D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone
keywords: Infertility, Intracytoplasmic sperm injection, follicle stimulating hormone, luteinizing hormone, FSH/LH ratio.