title: Resveratrol Protects against Helicobacter pylori-Associated Gastritis by Combating Oxidative Stress.
abstract: Helicobacter pylori (H. pylori)-induced oxidative stress has been shown to play a very important role in the inflammation of the gastric mucosa and increases the risk of developing gastric cancer. Resveratrol has many biological functions and activities, including antioxidant and anti-inflammatory effect. The purpose of this study was to probe whether resveratrol inhibits H. pylori-induced gastric inflammation and to elucidate the underlying mechanisms of any effect in mice. A mouse model of H. pylori infection was established via oral inoculation with H. pylori. After one week, mice were administered resveratrol (100 mg/kg body weight/day) orally for six weeks. The mRNA and protein levels of iNOS and IL-8 were assessed using RT-PCR, Western blot and ELISA. The expression levels of IκBα and phosphorylated IκBα (which embodies the level and activation of NF-κB), Heme Oxygenase-1 (HO-1; a potent antioxidant enzyme) and nuclear factor-erythroid 2 related factor 2 (Nrf2) were determined using Western blot, and lipid peroxide (LPO) level and myeloperoxidase (MPO) activity were examined using an MPO colorimetric activity assay, thiobarbituric acid reaction, and histological-grade using HE staining of the gastric mucosa. The results showed that resveratrol improved the histological infiltration score and decreased LPO level and MPO activity in the gastric mucosa. Resveratrol down-regulated the H. pylori-induced mRNA transcription and protein expression levels of IL-8 and iNOS, suppressed H. pylori-induced phosphorylation of IκBα, and increased the levels of HO-1 and Nrf2. In conclusion, resveratrol treatment exerted significant effects against oxidative stress and inflammation in H. pylori-infected mucosa through the suppression of IL-8, iNOS, and NF-κB, and moreover through the activation of the Nrf2/HO-1 pathway.
authors.affiliation: College of Food and Drug, University of Anhui Science and Technology, Bengbu 233100, China. zhangxlin@ahstu.edu.cn.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Zhang
authors.affiliation: The School of Life Science, University of Science and Technology of China, Hefei 230032, China. SA12226011@mail.ustc.edu.cn.
authors.forename: Anmin
authors.initials: A
authors.lastname: Jiang
authors.affiliation: College of Food and Drug, University of Anhui Science and Technology, Bengbu 233100, China. qibh@ahstu.edu.cn.
authors.forename: Banghua
authors.initials: B
authors.lastname: Qi
authors.affiliation: College of Food and Drug, University of Anhui Science and Technology, Bengbu 233100, China. mazy@ahstu.edu.cn.
authors.forename: Zhongyou
authors.initials: Z
authors.lastname: Ma
authors.affiliation: College of Food and Drug, University of Anhui Science and Technology, Bengbu 233100, China. xyytc1@163.com.
authors.forename: Youyi
authors.initials: Y
authors.lastname: Xiong
authors.affiliation: College of Food and Drug, University of Anhui Science and Technology, Bengbu 233100, China. doujf@ahstu.edu.cn.
authors.forename: Jinfeng
authors.initials: J
authors.lastname: Dou
authors.affiliation: College of Food and Drug, University of Anhui Science and Technology, Bengbu 233100, China. wangjf007@ahstu.edu.cn.|The Ministry of Agriculture Key Laboratory of Microbial Organic Fertilizer, Bengbu 233030, China. wangjf007@ahstu.edu.cn.
authors.forename: Jianfei
authors.initials: J
authors.lastname: Wang
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D004195:Disease Models, Animal; D005753:Gastric Mucosa / Q000187:drug effects / Q000378:metabolism / Q000382:microbiology / Q000473:pathology; D005756:Gastritis / Q000188:drug therapy / Q000209:etiology* / Q000378:metabolism*; D016481:Helicobacter Infections / Q000150:complications* / Q000382:microbiology; D016480:Helicobacter pylori; D051547:Heme Oxygenase-1 / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism; D008054:Lipid Peroxides / Q000378:metabolism; D008297:Male; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D009195:Peroxidase / Q000378:metabolism; D010766:Phosphorylation; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D000975:Antioxidants; D016209:Interleukin-8; D008054:Lipid Peroxides; D016328:NF-kappa B; D013267:Stilbenes; D009195:Peroxidase; D052247:Nitric Oxide Synthase Type II; D051547:Heme Oxygenase-1; D000077185:Resveratrol
keywords: HO-1; IL-8; Nrf2; gastritis; iNOS; oxidative stress; resveratrol