title: Genetic association of marbling score with intragenic nucleotide variants at selection signals of the bovine genome.
abstract: Selection signals of Korean cattle might be attributed largely to artificial selection for meat quality. Rapidly increased intragenic markers of newly annotated genes in the bovine genome would help overcome limited findings of genetic markers associated with meat quality at the selection signals in a previous study. The present study examined genetic associations of marbling score (MS) with intragenic nucleotide variants at selection signals of Korean cattle. A total of 39 092 nucleotide variants of 407 Korean cattle were utilized in the association analysis. A total of 129 variants were selected within newly annotated genes in the bovine genome. Their genetic associations were analyzed using the mixed model with random polygenic effects based on identical-by-state genetic relationships among animals in order to control for spurious associations produced by population structure. Genetic associations of MS were found (P<3.88×10-4) with six intragenic nucleotide variants on bovine autosomes 3 (cache domain containing 1, CACHD1), 5 (like-glycosyltransferase, LARGE), 16 (cell division cycle 42 binding protein kinase alpha, CDC42BPA) and 21 (snurportin 1, SNUPN; protein tyrosine phosphatase, non-receptor type 9, PTPN9; chondroitin sulfate proteoglycan 4, CSPG4). In particular, the genetic associations with CDC42BPA and LARGE were confirmed using an independent data set of Korean cattle. The results implied that allele frequencies of functional variants and their proximity variants have been augmented by directional selection for greater MS and remain selection signals in the bovine genome. Further studies of fine mapping would be useful to incorporate favorable alleles in marker-assisted selection for MS of Korean cattle.
authors.affiliation: Department of Bioinformatics and Life Science,Soongsil University,369 Sangdo-ro,Dongjak-gu,Seoul 156-743,Republic of Korea.
authors.forename: J
authors.initials: J
authors.lastname: Ryu
authors.affiliation: Department of Bioinformatics and Life Science,Soongsil University,369 Sangdo-ro,Dongjak-gu,Seoul 156-743,Republic of Korea.
authors.forename: C
authors.initials: C
authors.lastname: Lee
mesh_terms: D000483:Alleles; D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000235:genetics*; D005787:Gene Frequency; D056726:Genetic Association Studies; D005819:Genetic Markers / Q000235:genetics; D014644:Genetic Variation; D016678:Genome / Q000235:genetics*; D005838:Genotype; D008297:Male; D008460:Meat / Q000592:standards*
chemical_list: D005819:Genetic Markers
keywords: cattle; genetic marker; marbling score; meat quality; selection signature