title: Melamine Impairs Female Fertility via Suppressing Protein Level of Juno in Mouse Eggs.
abstract: Melamine is an organic nitrogenous compound widely used as an industrial chemical, and it has been recently reported by us that melamine has a toxic effect on the female reproductive system in mice, and renders females subfertile; the molecular basis, however, has not been adequately assessed. In the present study, we explore the underlying mechanism regarding how melamine compromises fertility in the mouse. The data showed that melamine exposure significantly impaired the fertilization capability of the egg during in vitro fertilization. To further figure out the cause, we analyzed ovastacin localization and protein level, the sperm binding ability of zona pellucida, and ZP2 cleavage status in unfertilized eggs from melamine fed mice, and no obvious differences were found between control and treatment groups. However, the protein level of Juno on the egg plasma membrane in the high-dose feeding group indeed significantly decreased compared to the control group. Thus, these data suggest that melamine compromises female fertility via suppressing Juno protein level on the egg membrane.
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 20095, China.
authors.forename: Xiaoxin
authors.initials: X
authors.lastname: Dai
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 20095, China.
authors.forename: Mianqun
authors.initials: M
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 20095, China.
authors.forename: Yajuan
authors.initials: Y
authors.lastname: Lu
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 20095, China.
authors.forename: Yilong
authors.initials: Y
authors.lastname: Miao
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 20095, China.
authors.forename: Changyin
authors.initials: C
authors.lastname: Zhou
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 20095, China.
authors.forename: Shaochen
authors.initials: S
authors.lastname: Sun
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 20095, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Xiong
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D005260:Female; D005298:Fertility / Q000187:drug effects* / Q000502:physiology; D051379:Mice; D008813:Mice, Inbred ICR; D010063:Ovum / Q000187:drug effects* / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000378:metabolism*; D013084:Sperm-Ovum Interactions / Q000187:drug effects / Q000502:physiology; D014227:Triazines / Q000494:pharmacology*; D015044:Zona Pellucida / Q000187:drug effects / Q000378:metabolism
chemical_list: D011956:Receptors, Cell Surface; D014227:Triazines; C543634:folate receptor 4, mouse; C011907:melamine