title: Exopolysaccharides produced by Oenococcus oeni: From genomic and phenotypic analysis to technological valorization.
abstract: Oenococcus oeni (O. oeni), which is the main species that drives malolactic fermentation (FML), an essential step for wine microbial stabilization and quality improvement, is known to produce exopolysaccharides (EPS). Depending on the strain, these EPS can be soluble, remain attached to the cell or both. In the present study, fourteen strains were examined for eps gene content and EPS production capacities. Cell-linked and soluble heteropolysaccharides made of glucose, galactose and rhamnose, soluble β-glucan, and soluble dextran or levan were found, depending on the strain. The protective potential of either cell-linked heteropolysaccharides or dextrans produced was then studied during freeze drying of the bacterial strains.
authors.affiliation: Univ. Bordeaux, ISVV, EA 4577 Unité de recherche Oenologie, Institut polytechnique de Bordeaux, F-33140 Villenave d'Ornon, France. Electronic address: maroula.dimo@hotmail.com.
authors.forename: Maria
authors.initials: M
authors.lastname: Dimopoulou
authors.affiliation: Univ. Bordeaux, ISVV, EA 4577 Unité de recherche Oenologie, Institut polytechnique de Bordeaux, F-33140 Villenave d'Ornon, France.
authors.forename: Tiphaine
authors.initials: T
authors.lastname: Bardeau
authors.affiliation: Univ. Bordeaux, ISVV, EA 4577 Unité de recherche Oenologie, Institut polytechnique de Bordeaux, F-33140 Villenave d'Ornon, France.
authors.forename: Pierre-Yves
authors.initials: PY
authors.lastname: Ramonet
authors.affiliation: Univ. Bordeaux, ISVV, EA 4577 Unité de recherche Oenologie, Institut polytechnique de Bordeaux, F-33140 Villenave d'Ornon, France; INRA, ISVV, USC 1366 Œnologie, F-33140 Villenave d'Ornon, France.
authors.forename: Cécile
authors.initials: C
authors.lastname: Miot-Certier
authors.affiliation: Univ. Bordeaux, ISVV, EA 4577 Unité de recherche Oenologie, Institut polytechnique de Bordeaux, F-33140 Villenave d'Ornon, France; INRA, ISVV, USC 1366 Œnologie, F-33140 Villenave d'Ornon, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Claisse
authors.affiliation: INRA, UMR1083, Sciences pour l'œnologie, F-34060 Montpellier, France.
authors.forename: Thiery
authors.initials: T
authors.lastname: Doco
authors.affiliation: Univ. Bordeaux, Bordeaux Imaging Center, UMS 3420 CNRS - US4 INSERM, 146 rue Léo Saignat, 33076 Bordeaux, France.
authors.forename: Melina
authors.initials: M
authors.lastname: Petrel
authors.affiliation: Univ. Bordeaux, ISVV, EA 4577 Unité de recherche Oenologie, Institut polytechnique de Bordeaux, F-33140 Villenave d'Ornon, France.
authors.forename: Patrick
authors.initials: P
authors.lastname: Lucas
authors.affiliation: Univ. Bordeaux, ISVV, EA 4577 Unité de recherche Oenologie, Institut polytechnique de Bordeaux, F-33140 Villenave d'Ornon, France.
authors.forename: Marguerite
authors.initials: M
authors.lastname: Dols-Lafargue
mesh_terms: D016667:Bacterial Capsules / Q000737:chemistry / Q000648:ultrastructure; D005285:Fermentation / Q000502:physiology; D005612:Freeze Drying; D023281:Genomics; D046529:Microscopy, Electron, Transmission; D056585:Oenococcus / Q000737:chemistry* / Q000378:metabolism*; D010641:Phenotype; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification; D014920:Wine / Q000382:microbiology
chemical_list: D011135:Polysaccharides, Bacterial
keywords: Freeze drying; Malolactic starters; Oenococcus oeni; Polysaccharides