title: Optimized Ratiometric Fluorescent Probes by Peptide Self-Assembly.
abstract: We report in this study on optimized ratiometric fluorescent probes by peptide self-assembly. The resulting self-assembled nanoprobes show extraordinary stability in aqueous solutions and extremely low background fluorescence in buffer solutions. Our optimized probes with much bigger ratiometric fluorescence ratios also show an enhanced cellular uptake, lower background noise, and much brighter fluorescence signal in the cell experiment. Our study provides a versatile and very useful strategy to design and produce fluorescent probes with better performance.
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, and Tianjin Key Laboratory of Molecular Drug Research, and ‡Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, and Collaborative Innovation Center of Chemical Science and Engineering, Tianjin, Nankai University , Tianjin 300071, People's Republic of China.
authors.forename: Yanbin
authors.initials: Y
authors.lastname: Cai
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, and Tianjin Key Laboratory of Molecular Drug Research, and ‡Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, and Collaborative Innovation Center of Chemical Science and Engineering, Tianjin, Nankai University , Tianjin 300071, People's Republic of China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhan
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, and Tianjin Key Laboratory of Molecular Drug Research, and ‡Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, and Collaborative Innovation Center of Chemical Science and Engineering, Tianjin, Nankai University , Tianjin 300071, People's Republic of China.
authors.forename: Haosheng
authors.initials: H
authors.lastname: Shen
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, and Tianjin Key Laboratory of Molecular Drug Research, and ‡Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, and Collaborative Innovation Center of Chemical Science and Engineering, Tianjin, Nankai University , Tianjin 300071, People's Republic of China.
authors.forename: Duo
authors.initials: D
authors.lastname: Mao
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, and Tianjin Key Laboratory of Molecular Drug Research, and ‡Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, and Collaborative Innovation Center of Chemical Science and Engineering, Tianjin, Nankai University , Tianjin 300071, People's Republic of China.
authors.forename: Shenglu
authors.initials: S
authors.lastname: Ji
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, and Tianjin Key Laboratory of Molecular Drug Research, and ‡Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, and Collaborative Innovation Center of Chemical Science and Engineering, Tianjin, Nankai University , Tianjin 300071, People's Republic of China.
authors.forename: Ruihua
authors.initials: R
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, and Tianjin Key Laboratory of Molecular Drug Research, and ‡Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, and Collaborative Innovation Center of Chemical Science and Engineering, Tianjin, Nankai University , Tianjin 300071, People's Republic of China.
authors.forename: Bing
authors.initials: B
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, and Tianjin Key Laboratory of Molecular Drug Research, and ‡Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, and Collaborative Innovation Center of Chemical Science and Engineering, Tianjin, Nankai University , Tianjin 300071, People's Republic of China.
authors.forename: Deling
authors.initials: D
authors.lastname: Kong
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, and Tianjin Key Laboratory of Molecular Drug Research, and ‡Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, and Collaborative Innovation Center of Chemical Science and Engineering, Tianjin, Nankai University , Tianjin 300071, People's Republic of China.
authors.forename: Ling
authors.initials: L
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, and Tianjin Key Laboratory of Molecular Drug Research, and ‡Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, and Collaborative Innovation Center of Chemical Science and Engineering, Tianjin, Nankai University , Tianjin 300071, People's Republic of China.
authors.forename: Zhimou
authors.initials: Z
authors.lastname: Yang
mesh_terms: D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D006834:Hydrazines / Q000032:analysis; D006862:Hydrogen Sulfide / Q000032:analysis; D008856:Microscopy, Fluorescence; D015394:Molecular Structure; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D006834:Hydrazines; D010455:Peptides; C029424:hydrazine; D006862:Hydrogen Sulfide