title: Synthetic mRNA Reprogramming of Human Fibroblast Cells.
abstract: Reprogramming of somatic cells, such as skin fibroblasts, to pluripotency was first achieved by forced expression of four transcription factors using integrating retroviral or lentiviral vectors, which result in integration of exogenous DNA into cellular genome and present a formidable barrier to therapeutic application of induced pluripotent stem cells (iPSCs). To facilitate the translation of iPSC technology to clinical practice, mRNA reprogramming method that generates transgene-free iPSCs is a safe and efficient method, eliminating bio-containment concerns associated with viral vectors, as well as the need for weeks of screening of cells to confirm that viral material has been completely eliminated during cell passaging.
authors.affiliation: Stem Cell and Genetic Engineering Group, Department of Materials Engineering, Faculty of Engineering, Monash University-Clayton Campus, Wellington Road, Clayton, VIC, 3800, Australia. jun.liu@monash.edu.
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.affiliation: Stem Cell and Genetic Engineering Group, Department of Materials Engineering, Faculty of Engineering, Monash University, Clayton, VIC, 3800, Australia.|South Australian Research and Development Institute (SARDI), Turretfield Research Centre, Rosedale, SA, Australia.
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Verma
mesh_terms: D002454:Cell Differentiation; D002478:Cells, Cultured; D065150:Cellular Reprogramming; D018920:Coculture Techniques; D061252:Feeder Cells; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism*; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism; D012333:RNA, Messenger / Q000138:chemical synthesis / Q000235:genetics*; D014162:Transfection; D019076:Transgenes
chemical_list: D012333:RNA, Messenger
keywords: Induced pluripotent stem cells; Modified mRNA; Reprogramming; Transfection; Transgene-free