title: Analysis of Major Histocompatibility Complex (MHC) Immunopeptidomes Using Mass Spectrometry.
abstract: The myriad of peptides presented at the cell surface by class I and class II major histocompatibility complex (MHC) molecules are referred to as the immunopeptidome and are of great importance for basic and translational science. For basic science, the immunopeptidome is a critical component for understanding the immune system; for translational science, exact knowledge of the immunopeptidome can directly fuel and guide the development of next-generation vaccines and immunotherapies against autoimmunity, infectious diseases, and cancers. In this mini-review, we summarize established isolation techniques as well as emerging mass spectrometry-based platforms (i.e. SWATH-MS) to identify and quantify MHC-associated peptides. We also highlight selected biological applications and discuss important current technical limitations that need to be solved to accelerate the development of this field.
authors.affiliation: From the ‡Department of Biology, Institute of Molecular Systems Biology, ETH Zurich, Zurich, Switzerland; caron@imsb.biol.ethz.ch aebersold@imsb.biol.ethz.ch.
authors.forename: Etienne
authors.initials: E
authors.lastname: Caron
authors.affiliation: §Department of Immunology, Interfaculty Institute for Cell Biology, University of Tübingen, Tübingen, Germany;
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Kowalewski
authors.affiliation: From the ‡Department of Biology, Institute of Molecular Systems Biology, ETH Zurich, Zurich, Switzerland;
authors.forename: Ching
authors.initials: C
authors.lastname: Chiek Koh
authors.affiliation: From the ‡Department of Biology, Institute of Molecular Systems Biology, ETH Zurich, Zurich, Switzerland;
authors.forename: Theo
authors.initials: T
authors.lastname: Sturm
authors.affiliation: §Department of Immunology, Interfaculty Institute for Cell Biology, University of Tübingen, Tübingen, Germany;
authors.forename: Heiko
authors.initials: H
authors.lastname: Schuster
authors.affiliation: From the ‡Department of Biology, Institute of Molecular Systems Biology, ETH Zurich, Zurich, Switzerland; ¶Faculty of Science, University of Zurich, Zurich, Switzerland caron@imsb.biol.ethz.ch aebersold@imsb.biol.ethz.ch.
authors.forename: Ruedi
authors.initials: R
authors.lastname: Aebersold
mesh_terms: D002462:Cell Membrane / Q000378:metabolism; D015395:Histocompatibility Antigens Class I / Q000737:chemistry / Q000378:metabolism*; D000949:Histocompatibility Antigens Class II / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D040901:Proteomics / Q000295:instrumentation / Q000379:methods
chemical_list: D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D010455:Peptides