title: Generation of Viable Mice from Induced Pluripotent Stem Cells (iPSCs) Through Tetraploid Complementation.
abstract: Tetraploid complementation assay is the most rigorous criteria for pluripotency characterization of pluripotent stem cells including embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). Pluripotent stem cells could complement the developmental deficiency of tetraploid embryos and thus support the full-term mice development. Here we describe the protocol for tetraploid complementation using iPSCs to produce viable all-iPSC mice.
authors.affiliation: Institute of Cancer Stem Cell, Dalian Medical University, Dalian, People's Republic of China.|National Institute of Biological Sciences, NIBS, #7 Science Park Road, Zhongguancun Life Science Park, Beijing, 102206, People's Republic of China.
authors.forename: Lan
authors.initials: L
authors.lastname: Kang
authors.affiliation: National Institute of Biological Sciences, NIBS, #7 Science Park Road, Zhongguancun Life Science Park, Beijing, 102206, People's Republic of China. gaoshaorong@nibs.ac.cn.|School of Life Sciences and Technology, Tongji University, Shanghai, People's Republic of China. gaoshaorong@nibs.ac.cn.
authors.forename: Shaorong
authors.initials: S
authors.lastname: Gao
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000378:metabolism; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D019976:Cloning, Organism; D004622:Embryo, Mammalian; D047108:Embryonic Development; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism*; D051379:Mice; D008845:Microinjections; D057891:Tetraploidy
keywords: Induced pluripotent stem cells (iPSCs); Pluripotency; Tetraploid complementation