title: Activation of AMPA receptor in the infralimbic cortex facilitates extinction and attenuates the heroin-seeking behavior in rats.
abstract: Infralimbic cortex (IL) is proposed to suppress cocaine seeking after extinction, but whether the IL regulates the extinction and reinstatement of heroin-seeking behavior is unknown. To address this issue, the male SD rats were trained to self-administer heroin under a FR1 schedule for consecutive 14 days, then the rats underwent 7 daily 2h extinction session in the operant chamber. The activation of IL by microinjection PEPA, an allosteric AMPA receptor potentiator into IL before each of extinction session facilitated the extinction responding after heroin self-administration, but did not alter the locomotor activity in an open field testing environment. Other rats were first trained under a FR1 schedule for heroin self-administration for 14 days, followed by 14 days of extinction training, and reinstatement of heroin-seeking induced by cues was measured for 2h. Intra-IL microinjecting of PEPA at 15min prior to test inhibited the reinstatement of heroin-seeking induced by cues. Moreover, the expression of GluR1 in the IL and NAc remarkably increased after treatment with PEPA during the reinstatement. These finding suggested that activation of glutamatergic projection from IL to NAc shell may be involved in the extinction and reinstatement of heroin-seeking.
authors.affiliation: Laboratory of Behavioral Neuroscience, Ningbo Institute of Microcirculation and Henbane, Ningbo Addiction Research and Treatment Center, School of Medicine, Ningbo University, Ningbo 315010, Zhejiang Province, PR China.
authors.forename: Weisheng
authors.initials: W
authors.lastname: Chen
authors.affiliation: Yinzhou Tongji High School, Ningbo 315175, Zhejiang Province, PR China.
authors.forename: Yiqi
authors.initials: Y
authors.lastname: Wang
authors.affiliation: The Children's Hospital Zhejiang University School of Medicine, Hangzhou 310003, Zhejiang Province, PR China.
authors.forename: Anna
authors.initials: A
authors.lastname: Sun
authors.affiliation: Hwa Chong Institution, 661 Bukit Timah Road, Singapore 269734, Singapore.
authors.forename: Linyi
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Laboratory of Behavioral Neuroscience, Ningbo Institute of Microcirculation and Henbane, Ningbo Addiction Research and Treatment Center, School of Medicine, Ningbo University, Ningbo 315010, Zhejiang Province, PR China.
authors.forename: Wenjin
authors.initials: W
authors.lastname: Xu
authors.affiliation: Laboratory of Behavioral Neuroscience, Ningbo Institute of Microcirculation and Henbane, Ningbo Addiction Research and Treatment Center, School of Medicine, Ningbo University, Ningbo 315010, Zhejiang Province, PR China.
authors.forename: Huaqiang
authors.initials: H
authors.lastname: Zhu
authors.affiliation: Laboratory of Behavioral Neuroscience, Ningbo Institute of Microcirculation and Henbane, Ningbo Addiction Research and Treatment Center, School of Medicine, Ningbo University, Ningbo 315010, Zhejiang Province, PR China.
authors.forename: Dingding
authors.initials: D
authors.lastname: Zhuang
authors.affiliation: Laboratory of Behavioral Neuroscience, Ningbo Institute of Microcirculation and Henbane, Ningbo Addiction Research and Treatment Center, School of Medicine, Ningbo University, Ningbo 315010, Zhejiang Province, PR China.
authors.forename: Miaojun
authors.initials: M
authors.lastname: Lai
authors.affiliation: Laboratory of Behavioral Neuroscience, Ningbo Institute of Microcirculation and Henbane, Ningbo Addiction Research and Treatment Center, School of Medicine, Ningbo University, Ningbo 315010, Zhejiang Province, PR China.
authors.forename: Fuqiang
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Laboratory of Behavioral Neuroscience, Ningbo Institute of Microcirculation and Henbane, Ningbo Addiction Research and Treatment Center, School of Medicine, Ningbo University, Ningbo 315010, Zhejiang Province, PR China. Electronic address: whzhou@vip.163.com.
authors.forename: Wenhua
authors.initials: W
authors.lastname: Zhou
authors.affiliation: Laboratory of Behavioral Neuroscience, Ningbo Institute of Microcirculation and Henbane, Ningbo Addiction Research and Treatment Center, School of Medicine, Ningbo University, Ningbo 315010, Zhejiang Province, PR China. Electronic address: lihufen@163.com.
authors.forename: Huifen
authors.initials: H
authors.lastname: Liu
mesh_terms: D000494:Allosteric Regulation; D000818:Animals; D058999:Drug-Seeking Behavior / Q000187:drug effects*; D005108:Extinction, Psychological / Q000187:drug effects*; D003932:Heroin / Q000008:administration & dosage*; D008297:Male; D008845:Microinjections; D009714:Nucleus Accumbens / Q000378:metabolism; D010642:Phenoxyacetates / Q000494:pharmacology; D017397:Prefrontal Cortex / Q000187:drug effects* / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D018091:Receptors, AMPA / Q000819:agonists* / Q000378:metabolism; D012646:Self Administration
chemical_list: C108189:4-(2-(phenylsulfonylamino)ethylthio)-2,6-difluorophenoxyacetamide; D010642:Phenoxyacetates; D018091:Receptors, AMPA; D003932:Heroin
keywords: AMPA receptor; Extinction; Frontal cortex; Heroin; Inhibitory memory