title: Transplantation of VEGFl65-overexpressing vascular endothelial progenitor cells relieves endothelial injury after deep vein thrombectomy.
abstract: The purpose of this study was to explore the therapeutic efficacy of VEGF165-overexpressing vascular endothelial progenitor cells (EPCs) in post-thrombotic syndrome.
  
  
  
  A thrombus model was developed to mimic the in-vivo setting, and adenovirus transfection was used to overexpress VEGF165 in EPCs. These cells were transplanted into the animal model, and their ability to relieve endothelial injury was evaluated using haematoxylin and eosin staining, immunohistochemistry and scanning electron microscopy.
  
  
  
  Ferric chloride was used to build rat models of the inferior vena cava thrombosis, and HEK 293A cells were used to amplify adenovirus that overexpresses VEGF165. EPCs were infected with adenovirus, and this was confirmed by fluorescence microscopy. Transplantation of VEGH165-overexpressing EPCs into injured endothelial sites led to faster repair of the post-thrombotic tunica intima than wild-type EPCs.
  
  
  
  Transplantation of VEGF165-overexpressing EPCs was found to promote repair of the tunica intima, thus improving rehabilitation after surgery.
authors.affiliation: Department of Vascular Surgery, Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China; Department of Vascular Surgery, Taihu Hospital, Wuxi, Jiangsu, China.
authors.forename: Jun-Jian
authors.initials: JJ
authors.lastname: Tang
authors.affiliation: Department of Vascular Surgery, Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China.
authors.forename: Qing-You
authors.initials: QY
authors.lastname: Meng
authors.affiliation: Department of Vascular Surgery, Taihu Hospital, Wuxi, Jiangsu, China.
authors.forename: Zhi-Xin
authors.initials: ZX
authors.lastname: Cai
authors.affiliation: Department of Vascular Surgery, Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China. Electronic address: flytsg1962@126.com.
authors.forename: Xiao-Qiang
authors.initials: XQ
authors.lastname: Li
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003131:Combined Modality Therapy / Q000379:methods; D042783:Endothelial Cells / Q000378:metabolism / Q000637:transplantation*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D033581:Stem Cell Transplantation / Q000379:methods*; D013234:Stem Cells / Q000378:metabolism; D017131:Thrombectomy / Q000009:adverse effects*; D016896:Treatment Outcome; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism*; D057772:Vascular System Injuries / Q000209:etiology / Q000378:metabolism / Q000517:prevention & control*; D020246:Venous Thrombosis / Q000628:therapy*
chemical_list: C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A
keywords: Adenovirus; Endothelial progenitor cells; Endothelium injury; Gene transfection; Vascular endothelial growth factor; Vein thrombectomy