title: Coagulation factor II from rock bream (Oplegnathus fasciatus): First report on the molecular biological function and expression analysis in the teleost.
abstract: The rapid haemostasis of fish prevents bleeding or infection that could be caused by physical properties of the aquatic environment. Additionally, the innate immune system is the first line of defence against infection and is responsible for the recognition of pathogen-associated molecular patterns, which are important for the activation of acquired immune responses. Coagulation factor II (CFII) is an important factor in the coagulation system and is involved in recognition and interaction with various bacterial and extracellular proteins. In this study, we identified and characterised the gene encoding CFII in rock bream (Oplegnathus fasciatus) (RbCFII) and analysed its expression in various tissues after a pathogen challenge. The full-length RbCFII cDNA (2079 bp) contained an open reading frame of 1854 bp encoding 617 amino acids. Alignment analysis revealed that a gamma-carboxyglutamic acid-rich domain, two kringle domains, and a trypsin-like serine protease domain of the deduced protein were well conserved. RbCFII was ubiquitously expressed in all tissues examined but, predominantly detected in the liver and skin. RbCFII expression was dramatically up-regulated in the kidney, spleen and liver after infection with Edwardsiella tarda, Streptococcus iniae, or red seabream iridovirus. The recombinant protein RbCFII (rRbCFII) produced using an Escherichia coli expression system was able to bind all examined bacteria. Interestingly, rRbCFII has agglutination activities towards E. coli and E. tarda, while no agglutination was shown toward Vibrio ordalii and S. iniae. These findings indicate that rRbCFII performs an immunological function in the immune response, and might be involved in innate immunity as well as blood coagulation.
authors.affiliation: Department of Marine Biology and Aquaculture, College of Marine Science, Gyeongsang National University, 38 Cheondaegukchi-Gil, Tongyeong, Gyeongnam 650-160, Republic of Korea.
authors.forename: Kwang-Min
authors.initials: KM
authors.lastname: Choi
authors.affiliation: Department of Marine Biology and Aquaculture, College of Marine Science, Gyeongsang National University, 38 Cheondaegukchi-Gil, Tongyeong, Gyeongnam 650-160, Republic of Korea.
authors.forename: Ji-Min
authors.initials: JM
authors.lastname: Jeong
authors.affiliation: Department of Marine Biology and Aquaculture, College of Marine Science, Gyeongsang National University, 38 Cheondaegukchi-Gil, Tongyeong, Gyeongnam 650-160, Republic of Korea.
authors.forename: Jin-Sol
authors.initials: JS
authors.lastname: Bae
authors.affiliation: Department of Marine Biology and Aquaculture, College of Marine Science, Gyeongsang National University, 38 Cheondaegukchi-Gil, Tongyeong, Gyeongnam 650-160, Republic of Korea.
authors.forename: Dong-Hee
authors.initials: DH
authors.lastname: Cho
authors.affiliation: Pathology Division, National Fisheries Research and Development Institute, Busan 619-900, Republic of Korea.
authors.forename: Sung Hee
authors.initials: SH
authors.lastname: Jung
authors.affiliation: Pathology Division, National Fisheries Research and Development Institute, Busan 619-900, Republic of Korea.
authors.forename: Jee-Youn
authors.initials: JY
authors.lastname: Hwang
authors.affiliation: Department of Marine Biology and Aquaculture, College of Marine Science, Gyeongsang National University, 38 Cheondaegukchi-Gil, Tongyeong, Gyeongnam 650-160, Republic of Korea.
authors.forename: Gun-Wook
authors.initials: GW
authors.lastname: Baeck
authors.affiliation: Department of Marine Biology and Aquaculture, College of Marine Science, Gyeongsang National University, 38 Cheondaegukchi-Gil, Tongyeong, Gyeongnam 650-160, Republic of Korea. Electronic address: vinus96@hanmail.net.
authors.forename: Chan-Il
authors.initials: CI
authors.lastname: Park
mesh_terms: D000372:Agglutination Tests; D000818:Animals; D004266:DNA Virus Infections / Q000276:immunology / Q000662:veterinary; D018076:DNA, Complementary / Q000235:genetics; D020609:Edwardsiella tarda; D004756:Enterobacteriaceae Infections / Q000276:immunology / Q000662:veterinary; D005260:Female; D005393:Fish Diseases / Q000276:immunology; D029941:Fish Proteins / Q000235:genetics / Q000276:immunology; D017994:Iridovirus; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008807:Mice, Inbred BALB C; D010473:Perciformes / Q000235:genetics / Q000276:immunology / Q000382:microbiology / Q000821:virology; D010802:Phylogeny; D011516:Prothrombin / Q000235:genetics / Q000276:immunology; D012333:RNA, Messenger / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D017422:Sequence Analysis, DNA; D013154:Spleen / Q000378:metabolism; D013290:Streptococcal Infections / Q000276:immunology / Q000662:veterinary; D013291:Streptococcus
chemical_list: D018076:DNA, Complementary; D029941:Fish Proteins; D012333:RNA, Messenger; D011994:Recombinant Proteins; D011516:Prothrombin
keywords: Coagulation factor II; Coagulation system; Innate immunity; Oplegnathus fasciatus; Recombinant protein