title: Delayed Numerical Chromosome Aberrations in Human Fibroblasts by Low Dose of Radiation.
abstract: Radiation-induced genomic instability refers to a type of damage transmitted over many generations following irradiation. This delayed impact of radiation exposure may pose a high risk to human health and increases concern over the dose limit of radiation exposure for both the public and radiation workers. Therefore, the development of additional biomarkers is still needed for the detection of delayed responses following low doses of radiation exposure. In this study, we examined the effect of X-irradiation on delayed induction of numerical chromosomal aberrations in normal human fibroblasts irradiated with 20, 50 and 100 cGy of X-rays using the micronucleus-centromere assay. Frequencies of centromere negative- and positive-micronuclei, and aneuploidy of chromosome 1 and 4 were analyzed in the surviving cells at 28, 88 and 240 h after X-irradiation. X-irradiation increased the frequency of micronuclei (MN) in a dose-dependent manner in the cells at all measured time-points, but no significant differences in MN frequency among cell passages were observed. Aneuploid frequency of chromosomes 1 and 4 increased with radiation doses, and a significantly higher frequency of aneuploidy was observed in the surviving cells analyzed at 240 h compared to 28 h. These results indicate that low-dose of X-irradiation can induce delayed aneuploidy of chromosomes 1 and 4 in normal fibroblasts.
authors.affiliation: Department of Molecular Epidemiology, School of Public Health, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 151-742, Korea. yoonhee.cho@umontana.edu.|Current address: Department of Biomedical and Pharmaceutical Sciences, College of Health Professions and Biomedical Sciences, University of Montana, 32 Campus Drive, Missoula, MT 59812, USA. yoonhee.cho@umontana.edu.
authors.forename: Yoon Hee
authors.initials: YH
authors.lastname: Cho
authors.affiliation: Department of Preventive Medicine, School of Medicine, Jeju National University, 66 Jejudaehakno, Jeju-si, Jeju-do 690-756, Korea. suy0202@jejunu.ac.kr.
authors.forename: Su Young
authors.initials: SY
authors.lastname: Kim
authors.affiliation: Molecular Epidemiology Branch, Division of Cancer Epidemiology and Prevention, Research Institute, National Cancer Center, Goyang-si, Gyeonggi-do 410-769, Korea. eastsea93@hanmail.net.
authors.forename: Hae Dong
authors.initials: HD
authors.lastname: Woo
authors.affiliation: Da Vinci College of General Education, Chung-ang University, 84 HeukSeok-Ro, DongJak-gu, Seoul 156-756, Korea. yangjee4@hanmail.net.
authors.forename: Yang Jee
authors.initials: YJ
authors.lastname: Kim
authors.affiliation: Department of Radiation Oncology, Seoul National University College of Medicine, 101 Daehak-ro, Jongno-gu, Seoul 110-744, Korea. swha@snu.ac.kr.
authors.forename: Sung Whan
authors.initials: SW
authors.lastname: Ha
authors.affiliation: Department of Molecular Epidemiology, School of Public Health, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 151-742, Korea. chugnhw@snu.ac.kr.
authors.forename: Hai Won
authors.initials: HW
authors.lastname: Chung
mesh_terms: D000782:Aneuploidy; D002467:Cell Nucleus / Q000528:radiation effects*; D002470:Cell Survival / Q000528:radiation effects*; D002478:Cells, Cultured / Q000528:radiation effects; D002503:Centromere / Q000528:radiation effects*; D002869:Chromosome Aberrations / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D005347:Fibroblasts / Q000528:radiation effects*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D015162:Micronucleus Tests; D000069079:Radiation Exposure / Q000009:adverse effects*; D018570:Risk Assessment; D013997:Time Factors; D014965:X-Rays / Q000009:adverse effects*
keywords: X-irradiation; aneuploidy; micronuclei; micronucleus-centromere assay; radiation-induced genomic instability