title: Microarray analysis of gene expression in olive flounder liver infected with viral haemorrhagic septicaemia virus (VHSV).
abstract: The most fatal viral pathogen in olive flounder Paralichthys olivaceus, is viral hemorrhagic septicemia virus, which afflicts over 48 species of freshwater and marine fish. Here, we performed gene expression profiling on transcripts isolated from VHSV-infected olive flounder livers using a 13 K cDNA microarray chip. A total of 1832 and 1647 genes were upregulated and down-regulated over two-fold, respectively, after infection. A variety of immune-related genes showing significant changes in gene expression were identified in upregulated genes through gene ontology annotation. These genes were grouped into categories such as antibacterial peptide, antigen-recognition and adhesion molecules, apoptosis, cytokine-related pathway, immune system, stress response, and transcription factor and regulatory factors. To verify the cDNA microarray data, we performed quantitative real-time PCR, and the results were similar to the microarray data. In conclusion, these results may be useful for the identification of specific genes or for the diagnosis of VHSV infection in flounder.
authors.affiliation: Biotechnology Research Division, National Fisheries Research and Development Institute, Busan 46083, Republic of Korea.
authors.forename: Hyun Kook
authors.initials: HK
authors.lastname: Cho
authors.affiliation: Biotechnology Research Division, National Fisheries Research and Development Institute, Busan 46083, Republic of Korea.
authors.forename: Julan
authors.initials: J
authors.lastname: Kim
authors.affiliation: Biotechnology Research Division, National Fisheries Research and Development Institute, Busan 46083, Republic of Korea.
authors.forename: Ji Young
authors.initials: JY
authors.lastname: Moon
authors.affiliation: Biotechnology Research Division, National Fisheries Research and Development Institute, Busan 46083, Republic of Korea.
authors.forename: Bo-Hye
authors.initials: BH
authors.lastname: Nam
authors.affiliation: Biotechnology Research Division, National Fisheries Research and Development Institute, Busan 46083, Republic of Korea.
authors.forename: Young-Ok
authors.initials: YO
authors.lastname: Kim
authors.affiliation: Biotechnology Research Division, National Fisheries Research and Development Institute, Busan 46083, Republic of Korea.
authors.forename: Woo-Jin
authors.initials: WJ
authors.lastname: Kim
authors.affiliation: Biotechnology Research Division, National Fisheries Research and Development Institute, Busan 46083, Republic of Korea.
authors.forename: Jung Youn
authors.initials: JY
authors.lastname: Park
authors.affiliation: Biotechnology Research Division, National Fisheries Research and Development Institute, Busan 46083, Republic of Korea.
authors.forename: Cheul Min
authors.initials: CM
authors.lastname: An
authors.affiliation: Department of Molecular Biology, Pusan National University, Busan 46241, Republic of Korea.
authors.forename: Jaehun
authors.initials: J
authors.lastname: Cheong
authors.affiliation: Biotechnology Research Division, National Fisheries Research and Development Institute, Busan 46083, Republic of Korea. Electronic address: heejkong@korea.kr.
authors.forename: Hee Jeong
authors.initials: HJ
authors.lastname: Kong
mesh_terms: D000818:Animals; D029941:Fish Proteins / Q000235:genetics* / Q000378:metabolism; D005432:Flounder; D020869:Gene Expression Profiling / Q000662:veterinary; D005786:Gene Expression Regulation; D031941:Hemorrhagic Septicemia, Viral / Q000235:genetics* / Q000276:immunology*; D008099:Liver / Q000276:immunology / Q000821:virology; D029141:Novirhabdovirus / Q000502:physiology*; D020411:Oligonucleotide Array Sequence Analysis / Q000662:veterinary*; D017422:Sequence Analysis, DNA / Q000662:veterinary
chemical_list: D029941:Fish Proteins
keywords: Cell cycle; Energy metabolism; Gene expression; Immune response; Microarray analysis; Olive flounder; Paralichthys olivaceus; Viral hemorrhagic septicemia virus