title: Improved Antioxidant Capacity of Optimization of a Self-Microemulsifying Drug Delivery System for Resveratrol.
abstract: The use of nano-encapsulated resveratrol (RSV) in self-micro-emulsified drug delivery systems (SMEDDS) formulations was investigated. Self-emulsifying grading tests were used to establish the optimal ratio of oil, surfactant, and co-surfactant. The optimized system was further investigated for the droplet size and zeta potential at the different medium pH values by a Malvern Zetasizer and transmission electron microscopy (TEM). The antioxidant capacity and cytotoxicity of the formulation were detected by DCFH-DA and a CCK-8 assays. The results showed that the nano-emulsion based on ethyl oleate, Tween-80, and PEG-400 (35:40:25, w/w/w) was the most stable formulation due to the small droplet size (approximately 50 nm) and high zeta potential in a neutral environment. Furthermore, this formulation also exhibited a greater antioxidant capacity with less toxicity than free RSV. Taken together, considering these results and the simple fabrication process, this formulation could be used to deliver nutritional food supplements in a stable, efficient, and safe manner.
authors.affiliation: College of Life Sciences, Henan Normal University, Xinxiang 453007, China. yingch0816@163.com.
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.affiliation: College of Life Science and Biotechnology, Xinxiang Medical University, Xinxiang 453003, China. huiyongzhang115@163.com.
authors.forename: Huiyong
authors.initials: H
authors.lastname: Zhang
authors.affiliation: School of Life Science and Technology, Henan Institute of Science and Technology, Xinxiang 453003, China. yangjing_228@sohu.com.
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.affiliation: School of Life Science and Technology, Henan Institute of Science and Technology, Xinxiang 453003, China. haiyan20050101@163.com.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Sun
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D001682:Biological Availability; D049109:Cell Proliferation / Q000187:drug effects; D004337:Drug Carriers; D016503:Drug Delivery Systems; D004655:Emulsions; D016716:PC12 Cells; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism*; D000077185:Resveratrol; D013267:Stilbenes / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D004337:Drug Carriers; D004655:Emulsions; D017382:Reactive Oxygen Species; D013267:Stilbenes; D000077185:Resveratrol
keywords: antioxidant; cytotoxicity; nano-encapsulation; oil-in-water; resveratrol; self-emulsifying