title: Polymorphisms in the regulatory region of the porcine MYLPF gene are related to meat quality traits in the Large White breed.
abstract: The MYLPF gene encodes fast myosin regulatory light chain, and is a positional and functional candidate gene for meat quality. The aim of this study was to identify associations between SNPs in the promoter region of the porcine MYLPF gene and meat quality traits. A total of 22 SNPs were identified in a population of crossbred animals (n=86) and based on minor allele frequency and proximity to the transcription start site, five SNPs were genotyped in purebred; Large White (n=98), Duroc (n=99) and Pietrain (n=98) pigs. No associations were observed in the Pietrain breed, while the Duroc breed was almost monomorphic for all SNPs. In the Large White breed SNP g-1314A>G and linked SNPS g.-871T>G, g.-566T>C, g.-403C>G were associated with ultimate pH and driploss (P<0.05). This study identified associations between MYLPF and meat quality and highlights the importance of considering the genetic background within gene-assisted selection programmes.
authors.affiliation: School of Veterinary Medicine, University College Dublin, Belfield, Dublin 4, Ireland. Electronic address: marion.ryan@ucd.ie.
authors.forename: M T
authors.initials: MT
authors.lastname: Ryan
authors.affiliation: School of Veterinary Medicine, University College Dublin, Belfield, Dublin 4, Ireland.
authors.forename: A M
authors.initials: AM
authors.lastname: O'Halloran
authors.affiliation: Teagasc, Ashtown Food Research Centre, Ashtown Dublin 15, Ireland.
authors.forename: R M
authors.initials: RM
authors.lastname: Hamill
authors.affiliation: Functional Genomics & Glycomics Group, National University of Ireland Galway, Galway, Ireland.
authors.forename: G C
authors.initials: GC
authors.lastname: Davey
authors.affiliation: IRTA, Product Quality E-17121 Monells, Girona, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Gil
authors.affiliation: Genus PLC/PIC, Alpha Building, London Road, Nantwich CW5 7JW, UK.
authors.forename: O I
authors.initials: OI
authors.lastname: Southwood
authors.affiliation: School of Veterinary Medicine, University College Dublin, Belfield, Dublin 4, Ireland.
authors.forename: T
authors.initials: T
authors.lastname: Sweeney
mesh_terms: D000273:Adipose Tissue / Q000502:physiology; D000818:Animals; D005260:Female; D005786:Gene Expression Regulation; D008297:Male; D008460:Meat / Q000592:standards*; D018994:Myosin Light Chains / Q000235:genetics / Q000378:metabolism*; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D013552:Swine / Q000235:genetics / Q000502:physiology
chemical_list: D018994:Myosin Light Chains
keywords: Duroc; Glycolytic; HUMMLC2B; Myosin light chain; Pietrain