title: Antisense oligonucleotide delivery to cancer cell lines for the treatment of different cancer types.
abstract: Amphiphilic poly(3-hydroxylalkanoate) (PHA) copolymers find interesting applications in drug delivery. The aim of this study was to prepare nucleic acid adsorbed on (PHB-b-PEG-NH2) nanoparticle platform for gene delivery. For this purpose, PHB-b-PEG-NH2block copolymers were synthesized via transesterification reactions. The copolymers obtained were characterized by Proton Nuclear Magnetic Resonance (1H-NMR), Fourier Transform Infrared Spectrometer (FTIR), Thermogravimetric Analysis (TGA) and Differential Scanning Calorimetry (DSC) techniques. The cytotoxic, apoptotic and necrotic effects of these nanoparticles in the MDA 231 human breast cancer cell, the A549 human lung cancer cell and the L929 fibroblast cell lines were also investigated.
authors.affiliation: a Department of Electronic and Automation , Zonguldak Vocational School, Bülent Ecevit University , Kilimli , Zonguldak , Turkey.
authors.forename: Ebru
authors.initials: E
authors.lastname: Kilicay
authors.affiliation: b Department of Biology , Faculty of Science, Aksaray University , Aksaray , Turkey.
authors.forename: Ebru
authors.initials: E
authors.lastname: Erdal
authors.affiliation: c Department of Chemistry, Division of Physical Chemistry , Bülent Ecevit University , Beytepe , Zonguldak , Turkey.
authors.forename: Baki
authors.initials: B
authors.lastname: Hazer
authors.affiliation: d Department of Bioengineering , Faculty of Engineering, Kirikkale University , Kirikkale , Turkey.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Türk
authors.affiliation: e Department of Chemistry, Division of Biochemistry , Hacettepe University , Beytepe , Ankara , Turkey.
authors.forename: Emir Baki
authors.initials: EB
authors.lastname: Denkbas
mesh_terms: D000818:Animals; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D016503:Drug Delivery Systems / Q000379:methods*; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism; D051379:Mice; D020319:Oligodeoxyribonucleotides, Antisense / Q000494:pharmacology*; D000091402:Prohibitins
chemical_list: D020319:Oligodeoxyribonucleotides, Antisense; C000717328:PHB protein, human; D000091402:Prohibitins
keywords: Antisense oligonucleotide; PHB-PEG block copolymer; PHB-PEG nanoparticle; fibroblast cells; human breast cancer cells; human lung cancer cells