title: Effects of Lutein and Zeaxanthin on LPS-Induced Secretion of IL-8 by Uveal Melanocytes and Relevant Signal Pathways.
abstract: The effects of lutein and zeaxanthin on lipopolysaccharide- (LPS-) induced secretion of IL-8 by uveal melanocytes (UM) were tested in cultured human UM. MTT assay revealed that LPS (0.01-1 μg/mL) and lutein and zeaxanthin (1-10 μM) did not influence the cell viability of cultured UM. LPS caused a dose-dependent increase of secretion of IL-8 by cultured UM. Lutein and zeaxanthin did not affect the constitutive secretion of IL-8. However, lutein and zeaxanthin decreased LPS-induced secretion of IL-8 in cultured UM in a dose-dependent manner. LPS significantly increased NF-κB levels in cell nuclear extracts and p-JNK levels in the cell lysates from UM, but not p-p38 MAPK and p-ERG. Lutein or zeaxanthin significantly reduced LPS-induced increase of NF-κB and p-JNK levels, but not p38 MAPK and ERG levels. The present study demonstrated that lutein and zeaxanthin inhibited LPS-induced secretion of IL-8 in cultured UM via JNK and NF-κB signal pathways. The anti-inflammatory effects of lutein and zeaxanthin might be explored as a therapeutic approach in the management of uveitis and other inflammatory diseases of the eye.
authors.affiliation: Department of Ophthalmology, Show Chwan Memorial Hospital, No. 526, Sec. 1, Zhongshan Road, Changhua 500, Taiwan ; Institute of Electrical and Computer Engineering, National Chiao Tung University, Hsinchu 30010, Taiwan ; Central Taiwan University of Science and Technology, No. 666, Buzih Road, Beitun District, Taichung 40601, Taiwan.
authors.forename: Shih-Chun
authors.initials: SC
authors.lastname: Chao
authors.affiliation: Albert Einstein College of Medicine, Yeshiva University, 1300 Morris Park Avenue, Bronx, NY 10461, USA.
authors.forename: Tommaso
authors.initials: T
authors.lastname: Vagaggini
authors.affiliation: Department of Ophthalmology, Show Chwan Memorial Hospital, No. 526, Sec. 1, Zhongshan Road, Changhua 500, Taiwan.
authors.forename: Chan-Wei
authors.initials: CW
authors.lastname: Nien
authors.affiliation: Institute of Electrical and Computer Engineering, National Chiao Tung University, Hsinchu 30010, Taiwan.
authors.forename: Sheng-Chieh
authors.initials: SC
authors.lastname: Huang
authors.affiliation: Department of Ophthalmology, Show Chwan Memorial Hospital, No. 526, Sec. 1, Zhongshan Road, Changhua 500, Taiwan ; Department of Optometry, Yuan Pei University, Hsinchu 30015, Taiwan.
authors.forename: Hung-Yu
authors.initials: HY
authors.lastname: Lin