title: Penetration of resin-based materials into initial erosion lesion: A confocal microscopic study.
abstract: The application of resin-based materials is an alternative of treatment for eroded lesions. Nevertheless, there are no studies about the penetration of these materials into eroded lesion, which might affect its adhesion. Therefore, this study evaluated the penetration of four resin-based materials, with and without enamel etching. By using an in vitro protocol, types of treatment were studied at five levels (AdheSE(®) , Tetric N-Bond(®) , Single Bond 2(®) , Helioseal Clear(®) , Icon(®) ) and types of enamel etching in two levels (with and without). Materials were stained with 0.02 mg/mL ethanolic solution of tetramethylrhodamine isothiocyanate. Bovine enamel samples (4 × 4 mm) were immersed in 0.01 M HCl, pH 2.3, for 30 seconds to produce initial eroded lesions. Afterward, the materials were applied on half of sample enamel surface following the manufacturer's instructions. On the other half of sample, the materials were applied without etching the enamel. Materials penetration into the enamel was assessed by Confocal Laser Scanning Microscopy on reflection and fluorescence modes. The penetration depth (PD) was measured using ImageJ software. Data were analyzed by two-way ANOVA and Tukey test (P < 0.05). Regardless of the material, etched enamel resulted in higher PD than non-etched (P < 0.05). Icon(®) showed the highest PD in enamel followed by Helioseal Clear(®) (P < 0.05), with significant difference between them (P < 0.05) and no difference was found among AdheSE(®) , Tetric N-Bond(®) , and Single Bond 2(®) (P > 0.05). It can be concluded that prior enamel etching increased the materials penetration into eroded enamel and the Icon(®) -infiltrant presented highest penetration.
authors.affiliation: Department of Pediatric Dentistry Orthodontics and Public Health, Bauru School of Dentistry, University of São Paulo (FOB-USP), Alameda Dr. Octávio Pinheiro Brisolla 9-75, Bauru, São Paulo, 17012-101, Brazil.
authors.forename: Franciny Querobim
authors.initials: FQ
authors.lastname: Ionta
authors.affiliation: Department of Dentistry Endodontics and Dental Materials, Bauru School of Dentistry, University of São Paulo (FOB-USP), Alameda Dr. Octávio Pinheiro Brisolla 9-75, Bauru, São Paulo, 17012-101, Brazil.
authors.forename: Ana Paula
authors.initials: AP
authors.lastname: Boteon
authors.affiliation: Department of Pediatric Dentistry, Mineiros School of Dentistry, Center of Higher Education Rezende & Potrich (FAMA), Jau, São Paulo, Brazil.
authors.forename: Marcelo Juliano
authors.initials: MJ
authors.lastname: Moretto
authors.affiliation: Department of Operative Dentistry Endodontics and Dental Materials, Bauru School of Dentistry, University of São Paulo (FOB-USP), Alameda Dr. Octávio Pinheiro Brisolla 9-75, Bauru, São Paulo, 17012-101, Brazil.
authors.forename: Odair Bim
authors.initials: OB
authors.lastname: Júnior
authors.affiliation: Department of Pediatric Dentistry Orthodontics and Public Health, Bauru School of Dentistry, University of São Paulo (FOB-USP), Alameda Dr. Octávio Pinheiro Brisolla 9-75, Bauru, São Paulo, 17012-101, Brazil.
authors.forename: Heitor Marques
authors.initials: HM
authors.lastname: Honório
authors.affiliation: Department of Pediatric Dentistry Orthodontics and Public Health, Bauru School of Dentistry, University of São Paulo (FOB-USP), Alameda Dr. Octávio Pinheiro Brisolla 9-75, Bauru, São Paulo, 17012-101, Brazil.
authors.forename: Thiago Cruvinel
authors.initials: TC
authors.lastname: Silva
authors.affiliation: Department of Operative Dentistry Endodontics and Dental Materials, Bauru School of Dentistry, University of São Paulo (FOB-USP), Alameda Dr. Octávio Pinheiro Brisolla 9-75, Bauru, São Paulo, 17012-101, Brazil.
authors.forename: Linda
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Pediatric Dentistry Orthodontics and Public Health, Bauru School of Dentistry, University of São Paulo (FOB-USP), Alameda Dr. Octávio Pinheiro Brisolla 9-75, Bauru, São Paulo, 17012-101, Brazil.
authors.forename: Daniela
authors.initials: D
authors.lastname: Rios
mesh_terms: D000818:Animals; D017438:Bisphenol A-Glycidyl Methacrylate / Q000737:chemistry; D002417:Cattle; D003743:Dental Enamel / Q000737:chemistry; D006801:Humans; D018613:Microscopy, Confocal; D012117:Resins, Synthetic / Q000737:chemistry*; D014077:Tooth Erosion / Q000628:therapy*
chemical_list: D012117:Resins, Synthetic; C115612:single bond; D017438:Bisphenol A-Glycidyl Methacrylate
keywords: dental enamel; dental materials; icon infiltrant; microscopy confocal; tooth erosion