title: Reverse micellar extraction of bromelain from pineapple peel--Effect of surfactant structure.
abstract: Pineapple peel is generally disposed or used as compost. This study was focused on extracting bromelain from pineapple peel by using reverse micelles. It was found that gemini surfactant C12-8-C12·2Br (octamethylene-α,ω-bis(dimethyldodecylammonium bromide)) showed distinctive advantage over its monomeric counterpart DTAB (dodecyl trimethyl ammonium bromide); under optimized condition, the bromelain extracted with C12-8-C12·2Br reverse micelle had an activity recovery of 163% and a purification fold of 3.3, while when using DTAB reverse micelle, the activity recovery was 95% and the purification fold was 1.7. Therefore, the spacer of gemini surfactant should play a positive role in bromelain extraction and may suggest the potential of gemini surfactant in protein separation since it has been so far rarely used in relative experiments or technologies.
authors.affiliation: School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, Jiangsu 225002, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wan
authors.affiliation: School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, Jiangsu 225002, PR China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Guo
authors.affiliation: School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, Jiangsu 225002, PR China.
authors.forename: Zhitong
authors.initials: Z
authors.lastname: Miao
authors.affiliation: School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, Jiangsu 225002, PR China. Electronic address: guoxia@yzu.edu.cn.
authors.forename: Xia
authors.initials: X
authors.lastname: Guo
mesh_terms: D031230:Ananas / Q000737:chemistry*; D001963:Bromelains / Q000737:chemistry*; D001965:Bromides / Q000737:chemistry*; D008823:Micelles; D010936:Plant Extracts / Q000737:chemistry; D000644:Quaternary Ammonium Compounds / Q000737:chemistry*; D013329:Structure-Activity Relationship; D013501:Surface-Active Agents / Q000737:chemistry*
chemical_list: D001965:Bromides; D008823:Micelles; D010936:Plant Extracts; D000644:Quaternary Ammonium Compounds; D013501:Surface-Active Agents; D001963:Bromelains; C051470:ammonium bromide
keywords: Bromelain; Pineapple peel; Protein extraction; Reverse micelle; Surfactant