title: Monitoring Biopolymer Degradation by Taylor Dispersion Analysis.
abstract: This work aims at demonstrating the interest of modern Taylor dispersion analysis (TDA), performed in narrow internal diameter capillary, for monitoring biopolymer degradations. Hydrolytic and enzymatic degradations of dendrigraft poly-l-lysine taken as model compounds have been performed and monitored by TDA at different degradation times. Different approaches for the data processing of the taylorgrams are compared, including simple integration of the taylorgram, curve fitting with a finite number of Gaussian peaks, cumulant-like method and Constrained Regularized Linear Inversion approach. Valuable information on the kinetics of the enzymatic/hydrolytic degradation reactions and on the degradation process can be obtained by TDA.
authors.affiliation: Institut des Biomolécules Max Mousseron (IBMM, UMR 5247 CNRS, Université de Montpellier, Ecole Nationale Supérieure de Chimie de Montpellier), Campus Triolet, Place Eugène Bataillon, CC 1706, 34095 Montpellier Cedex 5, France.
authors.forename: Joseph
authors.initials: J
authors.lastname: Chamieh
authors.affiliation: Institut des Biomolécules Max Mousseron (IBMM, UMR 5247 CNRS, Université de Montpellier, Ecole Nationale Supérieure de Chimie de Montpellier), Campus Triolet, Place Eugène Bataillon, CC 1706, 34095 Montpellier Cedex 5, France.
authors.forename: Jean Philippe
authors.initials: JP
authors.lastname: Biron
authors.affiliation: Laboratoire Charles Coulomb (L2C), UMR 5221 CNRS, Université de Montpellier, Montpellier, F-France.
authors.forename: Luca
authors.initials: L
authors.lastname: Cipelletti
authors.affiliation: Institut des Biomolécules Max Mousseron (IBMM, UMR 5247 CNRS, Université de Montpellier, Ecole Nationale Supérieure de Chimie de Montpellier), Campus Triolet, Place Eugène Bataillon, CC 1706, 34095 Montpellier Cedex 5, France.
authors.forename: Hervé
authors.initials: H
authors.lastname: Cottet
mesh_terms: D004058:Diffusion; D006868:Hydrolysis; D007700:Kinetics; D011107:Polylysine / Q000737:chemistry*; D012212:Rheology / Q000295:instrumentation / Q000379:methods*
chemical_list: D011107:Polylysine