title: Lipopolysaccharide preconditioning prevents acceleration of kindling epileptogenesis induced by traumatic brain injury.
abstract: 10-20% of symptomatic epilepsies are post-traumatic. We examined effect of LPS preconditioning on epileptogenesis after controlled cortical impact (CCI). LPS (0.01, 0.1 and 0.5 mg/kg) was injected i.p. to rats 5 days before induction of CCI to parieto-temporal cortex. Kindling started 24h after CCI by i.p. injection of 30 mg/kg of pentylenetetrazole every other day until manifestation of 3 consecutive generalized seizures. CCI injury accelerated the rate of kindled seizures acquisition. LPS (0.1 and 0.5 mg/kg) prevented the acceleration of kindling. LPS preconditioning significantly decreased IL-1β and TNF-α over-expression and the number of damaged neurons in the hippocampus of traumatic rats.
authors.affiliation: Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran; Department of Neuroscience, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran; Department of Physiology and Pharmacology, Pasteur Institute of Iran, Tehran, Iran; Department of Basic Sciences, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Mansoureh
authors.initials: M
authors.lastname: Eslami
authors.affiliation: Department of Physiology and Pharmacology, Pasteur Institute of Iran, Tehran, Iran. Electronic address: sayyahm2@pasteur.ac.ir.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Sayyah
authors.affiliation: Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Mansoureh
authors.initials: M
authors.lastname: Soleimani
authors.affiliation: Shefa Neuroscience Research Center, Tehran, Iran.
authors.forename: Leila
authors.initials: L
authors.lastname: Alizadeh
authors.affiliation: Brain and Spinal cord injury Research Center, Neuroscience Institute, Imam Khomeini Hospital, Tehran University of Medical Sciences, Tehran, Iran; Department of Neuroscience, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran. Electronic address: mhadjighassem@tums.ac.ir.
authors.forename: Mahmoudreza
authors.initials: M
authors.lastname: Hadjighassem
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001930:Brain Injuries / Q000150:complications* / Q000188:drug therapy; D003292:Convulsants / Q000633:toxicity; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004334:Drug Administration Schedule; D004827:Epilepsy / Q000209:etiology* / Q000517:prevention & control*; D004926:Escherichia coli; D006624:Hippocampus / Q000187:drug effects / Q000473:pathology; D053583:Interleukin-1beta / Q000378:metabolism; D007696:Kindling, Neurologic / Q000187:drug effects; D008070:Lipopolysaccharides / Q000008:administration & dosage*; D008297:Male; D010433:Pentylenetetrazole / Q000633:toxicity; D051381:Rats; D017208:Rats, Wistar; D013702:Temporal Lobe / Q000187:drug effects / Q000473:pathology; D013997:Time Factors
chemical_list: D003292:Convulsants; D053583:Interleukin-1beta; D008070:Lipopolysaccharides; D010433:Pentylenetetrazole
keywords: Controlled cortical impact; Epileptogenesis; LPS preconditioning; PTZ kindling