title: Association between leukaemia inhibitory factor gene polymorphism and pregnancy outcomes after assisted reproduction techniques.
abstract: Certain gene polymorphisms are associated with implantation failure and pregnancy loss. Studies of leukaemia inhibitory factor (LIF) gene polymorphisms are scarce. The LIF single nucleotide polymorphism (SNP) thymine (T)/guanine (G) (rs929271) was studied in women to determine whether an association existed with pregnancy outcomes after intracytoplasmic sperm injection (ICSI); 411 women who underwent ICSI were recruited. DNA was extracted from the peripheral blood, and the LIF gene SNP T/G (rs929271) was genotyped using real-time polymerase chain reaction. Participants were divided into three groups according to their LIF genotype: T/T (n = 168), T/G (n = 202) and G/G (n = 41). All IVF and ICSI procedures were carried out under the same clinical and laboratory conditions. The ICSI cumulative results (from fresh plus frozen cycles) of each genotype group were analysed. The G/G genotype in women was associated with a higher implantation rate (T/T: 15.9%, T/G: 16.2%, G/G: 27.0%; P < 0.05), ongoing pregnancy rate/patient (T/T: 31.5%, T/G: 36.1%, G/G: 53.7%; P < 0.05) and ongoing pregnancy rate/transfer (T/T: 18.5%, T/G: 20.2%, G/G: 36.7%; P < 0.05). LIF SNP T/G (rs929271) seems to be a susceptibility biomarker capable of predicting implantation efficiency and pregnancy outcomes.
authors.affiliation: Center for Human Reproduction Prof Franco Jr., Ribeirao Preto, SP, Brazil; Paulista Center for Diagnosis Research and Training, Ribeirao Preto, SP, Brazil.
authors.forename: Joao Batista A
authors.initials: JB
authors.lastname: Oliveira
authors.affiliation: Paulista Center for Diagnosis Research and Training, Ribeirao Preto, SP, Brazil.
authors.forename: Laura D
authors.initials: LD
authors.lastname: Vagnini
authors.affiliation: Center for Human Reproduction Prof Franco Jr., Ribeirao Preto, SP, Brazil; Paulista Center for Diagnosis Research and Training, Ribeirao Preto, SP, Brazil.
authors.forename: Claudia G
authors.initials: CG
authors.lastname: Petersen
authors.affiliation: Paulista Center for Diagnosis Research and Training, Ribeirao Preto, SP, Brazil.
authors.forename: Adriana
authors.initials: A
authors.lastname: Renzi
authors.affiliation: Paulista Center for Diagnosis Research and Training, Ribeirao Preto, SP, Brazil.
authors.forename: Gabriela R
authors.initials: GR
authors.lastname: Oliveira-Pelegrin
authors.affiliation: Center for Human Reproduction Prof Franco Jr., Ribeirao Preto, SP, Brazil; Paulista Center for Diagnosis Research and Training, Ribeirao Preto, SP, Brazil.
authors.forename: Ana L
authors.initials: AL
authors.lastname: Mauri
authors.affiliation: Center for Human Reproduction Prof Franco Jr., Ribeirao Preto, SP, Brazil.
authors.forename: Juliana
authors.initials: J
authors.lastname: Ricci
authors.affiliation: Center for Human Reproduction Prof Franco Jr., Ribeirao Preto, SP, Brazil.
authors.forename: Fabiana C
authors.initials: FC
authors.lastname: Massaro
authors.affiliation: Center for Human Reproduction Prof Franco Jr., Ribeirao Preto, SP, Brazil; Paulista Center for Diagnosis Research and Training, Ribeirao Preto, SP, Brazil.
authors.forename: Felipe
authors.initials: F
authors.lastname: Dieamant
authors.affiliation: Center for Human Reproduction Prof Franco Jr., Ribeirao Preto, SP, Brazil; Paulista Center for Diagnosis Research and Training, Ribeirao Preto, SP, Brazil; Women's Health Reference Center, Perola Byington Hospital, Sao Paulo, SP, Brazil.
authors.forename: Mario
authors.initials: M
authors.lastname: Cavagna
authors.affiliation: Center for Human Reproduction Prof Franco Jr., Ribeirao Preto, SP, Brazil; Paulista Center for Diagnosis Research and Training, Ribeirao Preto, SP, Brazil.
authors.forename: Ricardo L R
authors.initials: RL
authors.lastname: Baruffi
authors.affiliation: Center for Human Reproduction Prof Franco Jr., Ribeirao Preto, SP, Brazil; Paulista Center for Diagnosis Research and Training, Ribeirao Preto, SP, Brazil. Electronic address: crh@crh.com.br.
authors.forename: Jose G
authors.initials: JG
authors.lastname: Franco
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D010064:Embryo Implantation / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007246:Infertility / Q000453:epidemiology / Q000235:genetics / Q000628:therapy; D053605:Leukemia Inhibitory Factor / Q000235:genetics*; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D011247:Pregnancy; D011256:Pregnancy Outcome / Q000453:epidemiology / Q000235:genetics*; D018873:Pregnancy Rate; D027724:Reproductive Techniques, Assisted / Q000706:statistics & numerical data
chemical_list: C507837:LIF protein, human; D053605:Leukemia Inhibitory Factor
keywords: LIF T/G (rs929271) polymorphism; implantation; pregnancy; single nucleotide polymorphism; women's genotype