title: Nucleation and Dynamics of Golgi-derived Microtubules.
abstract: Integrity of the Golgi apparatus requires the microtubule (MT) network. A subset of MTs originates at the Golgi itself, which in this case functions as a MT-organizing center (MTOC). Golgi-derived MTs serve important roles in post-Golgi trafficking, maintenance of Golgi integrity, cell polarity and motility, as well as cell type-specific functions, including neurite outgrowth/branching. Here, we discuss possible models describing the formation and dynamics of Golgi-derived MTs. How Golgi-derived MTs are formed is not fully understood. A widely discussed model implicates that the critical step of the process is recruitment of molecular factors, which drive MT nucleation (γ-tubulin ring complex, or γ-TuRC), to the Golgi membrane via specific scaffolding interactions. Based on recent findings, we propose to introduce an additional level of regulation, whereby MT-binding proteins and/or local tubulin dimer concentration at the Golgi helps to overcome kinetic barriers at the initial nucleation step. According to our model, emerging MTs are subsequently stabilized by Golgi-associated MT-stabilizing proteins. We discuss molecular factors potentially involved in all three steps of MT formation. To preserve proper cell functioning, a balance must be maintained between MT subsets at the centrosome and the Golgi. Recent work has shown that certain centrosomal factors are important in maintaining this balance, suggesting a close connection between regulation of centrosomal and Golgi-derived MTs. Finally, we will discuss potential functions of Golgi-derived MTs based on their nucleation site location within a Golgi stack.
authors.affiliation: Department of Cell and Developmental Biology, Vanderbilt University Medical Center Nashville, TN, USA.
authors.forename: Anna A W M
authors.initials: AA
authors.lastname: Sanders
authors.affiliation: Department of Cell and Developmental Biology, Vanderbilt University Medical Center Nashville, TN, USA.
authors.forename: Irina
authors.initials: I
authors.lastname: Kaverina
keywords: CLASP; Golgi apparatus; Golgi-derived microtubules; microtubule dynamics; microtubule nucleation; γ-TuRC