title: Adoptive transfer of immune subsets prior to MCAO does not exacerbate stroke outcome in splenectomized mice.
abstract: The peripheral immune response contributes to neurologic impairment after stroke and the extent of initial damage is greater in males than females. We have previously shown that spleen cells directly contribute to ischemic damage in males, as splenectomy prior to experimental stroke eliminates the sex differences in infarct volume. This study aims to determine which specific subset of immune cells exert pathogenic effects when injected 24 hours before MCAO induction into splenectomized male and female WT mice. The results demonstrate that CD4/CD8/CD11b treated mice had no significant effect on infarct volumesvs. vehicle-treated control mice after MCAO. However, there were significant alterations to the resident peripheral immune composition. These results suggest that there are regulatory factors resulting from splenectomy or other possible influences that inhibit peripheral immune cell contribution to neuroinflammation and thus contributing to differential effects of the spleen on stroke outcome in males and female mice.
authors.affiliation: Department of Anesthesiology and Perioperative Medicine, Oregon Health and Science University, Portland, OR, USA.
authors.forename: Jianming
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Neurology, Oregon Health and Science University, Portland, OR, USA ; Neuroimmunology Research, VA Medical Center, Portland, OR, USA.
authors.forename: Abby L
authors.initials: AL
authors.lastname: Dotson
authors.affiliation: Department of Anesthesiology and Perioperative Medicine, Oregon Health and Science University, Portland, OR, USA ; Department of Behavioral Neuroscience, Oregon Health and Science University, Portland, OR, USA.
authors.forename: Stephanie J
authors.initials: SJ
authors.lastname: Murphy
authors.affiliation: Department of Anesthesiology and Perioperative Medicine, Oregon Health and Science University, Portland, OR, USA ; Department of Neurology, Oregon Health and Science University, Portland, OR, USA ; Neuroimmunology Research, VA Medical Center, Portland, OR, USA.
authors.forename: Halina
authors.initials: H
authors.lastname: Offner
authors.affiliation: Department of Anesthesiology and Perioperative Medicine, Oregon Health and Science University, Portland, OR, USA ; Department of Behavioral Neuroscience, Oregon Health and Science University, Portland, OR, USA ; Department of Medical and Molecular Genetics, Oregon Health and Science University, Portland, OR, USA.
authors.forename: Julie A
authors.initials: JA
authors.lastname: Saugstad
keywords: CD11b; CD4; CD8; experimental stroke; female mice; focal cerebral ischemia; immune modulation; inflammation; middle cerebral artery occlusion; splenectomy