title: Generation of Footprint-Free Induced Pluripotent Stem Cells from Human Fibroblasts Using Episomal Plasmid Vectors.
abstract: Human induced pluripotent stem cells (hiPSCs) have provided novel insights into the etiology of disease and are set to transform regenerative medicine and drug screening over the next decade. The generation of human iPSCs free of a genetic footprint of the reprogramming process is crucial for the realization of these potential uses. Here we describe in detail the generation of human iPSC from control and disease-carrying individuals' fibroblasts using episomal plasmids.
authors.affiliation: Stem Cell Engineering Group, Australian Institute for Bioengineering and Nanotechnology, University of Queensland, Corner College and Cooper Rds (Building 75), St. Lucia, Brisbane, QLD, 4072, Australia.
authors.forename: Dmitry A
authors.initials: DA
authors.lastname: Ovchinnikov
authors.affiliation: Stem Cell Engineering Group, Australian Institute for Bioengineering and Nanotechnology, University of Queensland, Corner College and Cooper Rds (Building 75), St. Lucia, Brisbane, QLD, 4072, Australia.
authors.forename: Jane
authors.initials: J
authors.lastname: Sun
authors.affiliation: Stem Cell Engineering Group, Australian Institute for Bioengineering and Nanotechnology, University of Queensland, Corner College and Cooper Rds (Building 75), St. Lucia, Brisbane, QLD, 4072, Australia. e.wolvetang@uq.edu.au.
authors.forename: Ernst J
authors.initials: EJ
authors.lastname: Wolvetang
mesh_terms: D018929:Cell Culture Techniques; D065150:Cellular Reprogramming; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism*; D015870:Gene Expression; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics*; D016133:Polymerase Chain Reaction; D014162:Transfection / Q000379:methods; D019076:Transgenes
keywords: Episomal plasmid vectors; Fibroblasts; Genomic integration; Human induced pluripotent stem cells; Reprogramming; Transfection