title: Sucrose metabolism gene families and their biological functions.
abstract: Sucrose, as the main product of photosynthesis, plays crucial roles in plant development. Although studies on general metabolism pathway were well documented, less information is available on the genome-wide identification of these genes, their expansion and evolutionary history as well as their biological functions. We focused on four sucrose metabolism related gene families including sucrose synthase, sucrose phosphate synthase, sucrose phosphate phosphatase and UDP-glucose pyrophosphorylase. These gene families exhibited different expansion and evolutionary history as their host genomes experienced differentiated rates of the whole genome duplication, tandem and segmental duplication, or mobile element mediated gene gain and loss. They were evolutionarily conserved under purifying selection among species and expression divergence played important roles for gene survival after expansion. However, we have detected recent positive selection during intra-species divergence. Overexpression of 15 sorghum genes in Arabidopsis revealed their roles in biomass accumulation, flowering time control, seed germination and response to high salinity and sugar stresses. Our studies uncovered the molecular mechanisms of gene expansion and evolution and also provided new insight into the role of positive selection in intra-species divergence. Overexpression data revealed novel biological functions of these genes in flowering time control and seed germination under normal and stress conditions.
authors.affiliation: Genome Structural Biology Group, Temasek Life Sciences Laboratory, National University of Singapore, Singapore 117604.|TLL-IOB Joint R&D Laboratory, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.
authors.forename: Shu-Ye
authors.initials: SY
authors.lastname: Jiang
authors.affiliation: TLL-IOB Joint R&D Laboratory, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.
authors.forename: Yun-Hua
authors.initials: YH
authors.lastname: Chi
authors.affiliation: TLL-IOB Joint R&D Laboratory, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.
authors.forename: Ji-Zhou
authors.initials: JZ
authors.lastname: Wang
authors.affiliation: TLL-IOB Joint R&D Laboratory, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.
authors.forename: Jun-Xia
authors.initials: JX
authors.lastname: Zhou
authors.affiliation: TLL-IOB Joint R&D Laboratory, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.
authors.forename: Yan-Song
authors.initials: YS
authors.lastname: Cheng
authors.affiliation: TLL-IOB Joint R&D Laboratory, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.
authors.forename: Bao-Lan
authors.initials: BL
authors.lastname: Zhang
authors.affiliation: Genome Structural Biology Group, Temasek Life Sciences Laboratory, National University of Singapore, Singapore 117604.
authors.forename: Ali
authors.initials: A
authors.lastname: Ma
authors.affiliation: Genome Structural Biology Group, Temasek Life Sciences Laboratory, National University of Singapore, Singapore 117604.
authors.forename: Jeevanandam
authors.initials: J
authors.lastname: Vanitha
authors.affiliation: Genome Structural Biology Group, Temasek Life Sciences Laboratory, National University of Singapore, Singapore 117604.|TLL-IOB Joint R&D Laboratory, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.
authors.forename: Srinivasan
authors.initials: S
authors.lastname: Ramachandran
mesh_terms: D018533:Biomass; D050260:Carbohydrate Metabolism / Q000235:genetics*; D004251:DNA Transposable Elements; D019143:Evolution, Molecular; D035264:Flowers; D020440:Gene Duplication; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D014644:Genetic Variation; D018745:Genome, Plant; D055106:Genome-Wide Association Study; D023281:Genomics / Q000379:methods; D018525:Germination / Q000235:genetics; D005810:Multigene Family; D010641:Phenotype; D010802:Phylogeny; D010944:Plants / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D030821:Plants, Genetically Modified; D012641:Selection, Genetic; D013312:Stress, Physiological / Q000235:genetics; D013395:Sucrose / Q000378:metabolism*
chemical_list: D004251:DNA Transposable Elements; D013395:Sucrose