title: Molecular identification of nontuberculous mycobacteria isolated from pyogenic bovine tissues in South Darfur State and Alsabalouga slaughterhouse at Omdurman area, Sudan.
abstract: This study identified nontuberculous mycobacteria (NTM) recovered from bovine pyogenic affections obtained at necropsy using the molecular target 16S-23S rDNA internal transcribed spacer region. Postmortem inspection of cattle was conducted at South Darfur State abattoirs and Alsabalouga Slaughterhouse at Omdurman area during 2007-2009. Specimens were examined for the presence of acid fast bacteria (AFB) using microscopic and standard culturing techniques. AFB were identified phenotypically and confirmed by 16S-23S rDNA ITS. Fifty nine NTM were recovered and confirmed as acid fast filaments out of 165 positive AFB specimens, of which 52 isolates were identified as bovine farcy causative agents, while 7 cultures were excluded due to drying. 16S-23S rDNA ITS of NTM revealed three different amplicons 500 bp. (32) isolates, 550 bp. (2) isolates and 600 bp. (14) isolates. Four isolates were contaminated.
authors.affiliation: Departments of Veterinary Medicine, Faculty of Agriculture and Veterinary Medicine, Qassim University, Saudi Arabia.
authors.forename: A E El
authors.initials: AE
authors.lastname: Tigani-Asil
authors.affiliation: Departments of Microbiology, Faculty of Veterinary Medicine, University of Khartoum, Sudan.
authors.forename: S M El
authors.initials: SM
authors.lastname: Sanousi
authors.affiliation: Department of Pathology, Veterinary Research laboratory - Nyala, National Veterinary Research Corporation, Sudan.
authors.forename: M A
authors.initials: MA
authors.lastname: Aljameel
authors.affiliation: Department of Tuberculosis, Institute of Tropical Diseases, Ministry of Science and Technology, Sudan.
authors.forename: H El
authors.initials: HE
authors.lastname: Beir
authors.affiliation: Department of Pathology, Faculty of Veterinary Science, University of Nyala, Sudan.
authors.forename: A
authors.initials: A
authors.lastname: Adam
authors.affiliation: Department of Pathology, Faculty of Veterinary Science, University of Nyala, Sudan.
authors.forename: M M
authors.initials: MM
authors.lastname: Abdallatif
authors.affiliation: Department of Pathology, Faculty of Veterinary Science, University of Nyala, Sudan.
authors.forename: M E
authors.initials: ME
authors.lastname: Hamid
keywords: Acid fast bacteria; Bovine farcy; Nontuberculous mycobacteria; Pyogenic infection