title: A Proteomics Approach to Investigate miR-153-3p and miR-205-5p Targets in Neuroblastoma Cells.
abstract: MicroRNAs are key regulators associated with numerous diseases. In HEK293 cells, miR-153-3p and miR-205-5p down-regulate alpha-synuclein (SNCA) and Leucine-rich repeat kinase 2 (LRRK2), two key proteins involved in Parkinson's disease (PD). We have used two-dimensional gel electrophoresis (2D-PAGE) coupled to mass spectrometry (MS) to identify a spectrum of miR-153-3p and miR-205-5p targets in neuronal SH-SY5Y cells. We overexpressed and inhibited both microRNAs in SH-SY5Y cells and through comparative proteomics profiling we quantified ~240 protein spots from each analysis. Combined, thirty-three protein spots were identified showing significant (p-value < 0.05) changes in abundance. Modulation of miR-153-3p resulted in seven up-regulated proteins and eight down-regulated proteins. miR-205 modulation resulted in twelve up-regulated proteins and six down-regulated proteins. Several of the proteins are associated with neuronal processes, including peroxiredoxin-2 and -4, cofilin-1, prefoldin 2, alpha-enolase, human nucleoside diphosphate kinase B (Nm23) and 14-3-3 protein epsilon. Many of the differentially expressed proteins are involved in diverse pathways including metabolism, neurotrophin signaling, actin cytoskeletal regulation, HIF-1 signaling and the proteasome indicating that miR-153-3p and miR-205-5p are involved in the regulation of a wide variety of biological processes in neuroblastoma cells.
authors.affiliation: Department of Biological Sciences, St. John's University, New York, NY, 11439, United States of America.
authors.forename: Ketan S
authors.initials: KS
authors.lastname: Patil
authors.affiliation: Department of Biological Sciences, St. John's University, New York, NY, 11439, United States of America.
authors.forename: Indranil
authors.initials: I
authors.lastname: Basak
authors.affiliation: Department of Biological Sciences, St. John's University, New York, NY, 11439, United States of America.
authors.forename: Ramavati
authors.initials: R
authors.lastname: Pal
authors.affiliation: Department of Chemistry, York College and the Graduate Center, The City University of New York, New York, NY, 11451, United States of America.
authors.forename: Hsin-Pin
authors.initials: HP
authors.lastname: Ho
authors.affiliation: Norwegian Center for Movement Disorders, Stavanger University Hospital, 4068, Stavanger, Norway.
authors.forename: Guido
authors.initials: G
authors.lastname: Alves
authors.affiliation: Department of Chemistry, York College and the Graduate Center, The City University of New York, New York, NY, 11451, United States of America.
authors.forename: Emmanuel J
authors.initials: EJ
authors.lastname: Chang
authors.affiliation: Norwegian Center for Movement Disorders, Stavanger University Hospital, 4068, Stavanger, Norway.
authors.forename: Jan Petter
authors.initials: JP
authors.lastname: Larsen
authors.affiliation: Department of Biological Sciences, St. John's University, New York, NY, 11439, United States of America.|Norwegian Center for Movement Disorders, Stavanger University Hospital, 4068, Stavanger, Norway.
authors.forename: Simon Geir
authors.initials: SG
authors.lastname: Møller
mesh_terms: D002453:Cell Cycle; D045744:Cell Line, Tumor; D015180:Electrophoresis, Gel, Two-Dimensional; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000378:metabolism*; D009447:Neuroblastoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D040901:Proteomics; D014158:Transcription, Genetic / Q000235:genetics
chemical_list: D035683:MicroRNAs; D009363:Neoplasm Proteins; C556500:MIRN153 microRNA, human; C534425:MIR205, human