title: Elucidating the Molecular Function of Human BOLA2 in GRX3-Dependent Anamorsin Maturation Pathway.
abstract: In eukaryotes, the interaction between members of the monothiol glutaredoxin family and members of the BolA-like protein family has been involved in iron metabolism. To investigate the still unknown functional role of the interaction between human glutaredoxin-3 (GRX3) and its protein partner BOLA2, we characterized at the atomic level the interaction of apo BOLA2 with the apo and holo states of GRX3 and studied the role of BOLA2 in the GRX3-dependent anamorsin maturation pathway. From these studies, it emerged that apo GRX3 and apo BOLA2 form a heterotrimeric complex, composed by two BOLA2 molecules and one GRX3 molecule. This complex is able to bind two [2Fe-2S](2+) clusters, each being bridged between a BOLA2 molecule and a monothiol glutaredoxin domain of GRX3, and to transfer both [2Fe-2S](2+) clusters to apo anamorsin producing its mature holo state. Collectively, the data suggest that the heterotrimeric complex can work as a [2Fe-2S](2+) cluster transfer component in cytosolic Fe/S protein maturation pathways.
authors.affiliation: Magnetic Resonance Center CERM, University of Florence , Via Luigi Sacconi 6, 50019, Sesto Fiorentino, Florence, Italy.|Department of Chemistry, University of Florence , Via della Lastruccia 3, 50019 Sesto Fiorentino, Florence, Italy.
authors.forename: Lucia
authors.initials: L
authors.lastname: Banci
authors.affiliation: Magnetic Resonance Center CERM, University of Florence , Via Luigi Sacconi 6, 50019, Sesto Fiorentino, Florence, Italy.|Department of Chemistry, University of Florence , Via della Lastruccia 3, 50019 Sesto Fiorentino, Florence, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Camponeschi
authors.affiliation: Magnetic Resonance Center CERM, University of Florence , Via Luigi Sacconi 6, 50019, Sesto Fiorentino, Florence, Italy.|Department of Chemistry, University of Florence , Via della Lastruccia 3, 50019 Sesto Fiorentino, Florence, Italy.
authors.forename: Simone
authors.initials: S
authors.lastname: Ciofi-Baffoni
authors.affiliation: Magnetic Resonance Center CERM, University of Florence , Via Luigi Sacconi 6, 50019, Sesto Fiorentino, Florence, Italy.|Department of Chemistry, University of Florence , Via della Lastruccia 3, 50019 Sesto Fiorentino, Florence, Italy.
authors.forename: Riccardo
authors.initials: R
authors.lastname: Muzzioli
mesh_terms: D002352:Carrier Proteins / Q000378:metabolism*; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism*; D011506:Proteins / Q000502:physiology*
chemical_list: C533596:BOLA2 protein, human; C489114:CIAPIN1 protein, human; D002352:Carrier Proteins; C404105:GLRX3 protein, human; D047908:Intracellular Signaling Peptides and Proteins; D011506:Proteins