title: Skeletal Myoblast Cell Sheet Implantation Ameliorates Both Systolic and Diastolic Cardiac Performance in Canine Dilated Cardiomyopathy Model.
abstract: BACKGROUND
  Improving both systolic and diastolic function may be the most important factor in treating heart failure. In this study, we hypothesized that cell-sheet transplantation could improve these function in the damaged heart.
  
  
  METHODS
  We generated a dilated cardiomyopathy model in beagles by continuous ventricle pacing at 240 beats per minute. After 4 weeks, the beagles underwent skeletal myoblast cell sheet transplantation (SMCST) or a sham operation, and rapid ventricle pacing continued for an additional 4 weeks. Six of the e8 beagles treated by SMCST were still alive 4 weeks after the procedure. We evaluated SMCST's cardiotherapeutic effects by comparing beagles treated by SMCST with beagles that underwent a sham operation (control, n = 5).
  
  
  RESULTS
  Diastolic function, as well as systolic function improved significantly in the SMCST group as compared with the sham group (control vs SMCST group, median [interquartile range]: E/E', 16 [0.9] vs 11 [1.0]; P < 0.001; tau, 47 [6.0] vs 36 [4.4] ms: P = 0.005. Ejection fraction, 22 (6.0) versus 46 (7.5) %, P < 0.001; end-systolic elastance, 2.5 (0.4) versus 8.2 (3.5) mm Hg/ml, P = 0.001). Histological examination revealed that the volume of collagen I and the collagen I/III ratio in the myocardium were significantly higher in the control than that in the SMCST group (collagen I, 6.0 [0.8] vs 2.6 [1.3]; P = 0.006; collagen I/III ratio, 4.8 [1.7] vs 1.2 [0.4]; P = 0.010).
  
  
  CONCLUSIONS
  The potential of SMCST to ameliorate both systolic and diastolic performance was proven. The SMCST may be an alternative therapy of conventional medical treatment in the dilated cardiomyopathy heart.
authors.affiliation: 1 Division of Cardiovascular Surgery, Kobe University Graduate School of Medicine, Kobe, Japan. 2 Division of Cardiovascular Surgery, Osaka University Graduate School of Medicine, Suita, Japan. 3 Department of Molecular Pathology, Graduate School of Medicine and Health Sciences, Osaka University Graduate School of Medicine, Osaka, Japan. 4 Division of Functional Diagnostics, Department of Health Sciences, Osaka University Graduate School of Medicine, Osaka, Japan. 5 Division of Biostatistics, Hyogo College of Medicine, Hyogo, Japan.
authors.forename: Tomonori
authors.initials: T
authors.lastname: Shirasaka
authors.forename: Shigeru
authors.initials: S
authors.lastname: Miyagawa
authors.forename: Satsuki
authors.initials: S
authors.lastname: Fukushima
authors.forename: Naomasa
authors.initials: N
authors.lastname: Kawaguchi
authors.forename: Satoshi
authors.initials: S
authors.lastname: Nakatani
authors.forename: Takashi
authors.initials: T
authors.lastname: Daimon
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Okita
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Sawa
mesh_terms: D000818:Animals; D002304:Cardiac Pacing, Artificial; D002311:Cardiomyopathy, Dilated / Q000175:diagnosis / Q000209:etiology / Q000378:metabolism / Q000503:physiopathology / Q000601:surgery*; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D024042:Collagen Type I / Q000378:metabolism; D024043:Collagen Type II / Q000378:metabolism; D003971:Diastole; D004195:Disease Models, Animal; D004285:Dogs; D005260:Female; D005355:Fibrosis; D032448:Myoblasts, Skeletal / Q000378:metabolism / Q000637:transplantation*; D009206:Myocardium / Q000378:metabolism / Q000473:pathology; D020127:Recovery of Function; D013318:Stroke Volume; D013599:Systole; D013997:Time Factors; D016277:Ventricular Function, Left; D020257:Ventricular Remodeling
chemical_list: D024042:Collagen Type I; D024043:Collagen Type II