title: Euterpe oleracea Mart.-Derived Polyphenols Protect Mice from Diet-Induced Obesity and Fatty Liver by Regulating Hepatic Lipogenesis and Cholesterol Excretion.
abstract: The aim of this study was to investigate the effect of a polyphenol-rich Açaí seed extract (ASE, 300 mg/kg-1d-1) on adiposity and hepatic steatosis in mice that were fed a high-fat (HF) diet and its underlying mechanisms based on hepatic lipid metabolism and oxidative stress. Four groups were studied: C57BL/6 mice that were fed with standard diet (10% fat, Control), 10% fat + ASE (ASE), 60% fat (HF), and 60% fat + ASE (HF + ASE) for 12 weeks. We evaluated the food intake, body weight gain, serum glucose and lipid profile, hepatic cholesterol and triacyglycerol (TG), hepatic expression of pAMPK, lipogenic proteins (SREBP-1c, pACC, ACC, HMG-CoA reductase) and cholesterol excretion transporters, ABCG5 and ABCG8. We also evaluated the steatosis in liver sections and oxidative stress. ASE reduced body weight gain, food intake, glucose levels, accumulation of cholesterol and TG in the liver, which was associated with a reduction of hepatic steatosis. The increased expressions of SREBP-1c and HMG-CoA reductase and reduced expressions of pAMPK and pACC/ACC in HF group were antagonized by ASE. The ABCG5 and ABCG8 transporters expressions were increased by the extract. The antioxidant effect of ASE was demonstrated in liver of HF mice by restoration of SOD, CAT and GPx activities and reduction of the increased levels of malondialdehyde and protein carbonylation. In conclusion, ASE substantially reduced the obesity and hepatic steatosis induced by HF diet by reducing lipogenesis, increasing cholesterol excretion and improving oxidative stress in the liver, providing a nutritional resource for prevention of obesity-related adiposity and hepatic steatosis.
authors.affiliation: Department of Pharmacology, Institute of Biology, State University of Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Paola Raquel B
authors.initials: PR
authors.lastname: de Oliveira
authors.affiliation: Department of Pharmacology, Institute of Biology, State University of Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Cristiane A
authors.initials: CA
authors.lastname: da Costa
authors.affiliation: Department of Pharmacology, Institute of Biology, State University of Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Graziele F
authors.initials: GF
authors.lastname: de Bem
authors.affiliation: Department of Pharmacology, Institute of Biology, State University of Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Viviane S C
authors.initials: VS
authors.lastname: Cordeiro
authors.affiliation: Department of Pharmacology, Institute of Biology, State University of Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Izabelle B
authors.initials: IB
authors.lastname: Santos
authors.affiliation: Department of Pharmacology, Institute of Biology, State University of Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Lenize C R M
authors.initials: LC
authors.lastname: de Carvalho
authors.affiliation: Department of Physiological Sciences, Institute of Biology, State University of Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Ellen Paula S
authors.initials: EP
authors.lastname: da Conceição
authors.affiliation: Department of Physiological Sciences, Institute of Biology, State University of Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Patrícia Cristina
authors.initials: PC
authors.lastname: Lisboa
authors.affiliation: Department of Pharmacology, Institute of Biology, State University of Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Dayane T
authors.initials: DT
authors.lastname: Ognibene
authors.affiliation: Department of Pharmacy, Federal University of Pará, Belém, Pará, Brazil.
authors.forename: Pergentino José C
authors.initials: PJ
authors.lastname: Sousa
authors.affiliation: IPPN, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Gabriel R
authors.initials: GR
authors.lastname: Martins
authors.affiliation: IPPN, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Antônio Jorge R
authors.initials: AJ
authors.lastname: da Silva
authors.affiliation: Department of Pharmacology, Institute of Biology, State University of Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Roberto S
authors.initials: RS
authors.lastname: de Moura
authors.affiliation: Department of Pharmacology, Institute of Biology, State University of Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Angela C
authors.initials: AC
authors.lastname: Resende
mesh_terms: D054392:Adipokines / Q000378:metabolism; D000273:Adipose Tissue / Q000187:drug effects / Q000473:pathology; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001835:Body Weight / Q000187:drug effects; D002784:Cholesterol / Q000096:biosynthesis / Q000378:metabolism*; D059305:Diet, High-Fat / Q000009:adverse effects; D004435:Eating / Q000187:drug effects; D065748:Euterpe / Q000737:chemistry*; D005227:Fatty Acids / Q000096:biosynthesis; D005786:Gene Expression Regulation / Q000187:drug effects; D050155:Lipogenesis / Q000187:drug effects*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D065626:Non-alcoholic Fatty Liver Disease / Q000209:etiology / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D009765:Obesity / Q000209:etiology / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D009929:Organ Size / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D059808:Polyphenols / Q000494:pharmacology*; D012639:Seeds / Q000737:chemistry
chemical_list: D054392:Adipokines; D000975:Antioxidants; D005227:Fatty Acids; D059808:Polyphenols; D002784:Cholesterol