title: Nucleotide sequence analysis of Vietnamese highly pathogenic porcine reproductive and respiratory syndrome virus from 2013 to 2014 based on the NSP2 and ORF5 coding regions.
abstract: A total of 34 highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) strains isolated from Vietnam during 2013-2014 were sequenced and analyzed. A partial sequence of ORF1a corresponding to the nonstructural protein 2 (Nsp2) coding region and the full sequence of open reading frame 5 (ORF5) gene was used for the analysis. The HP-PRRSV strains were isolated from pig herds that had never been vaccinated for PRRSV. Nucleotide sequence identities in the portions of ORF1a corresponding to the nonstructural protein 2 (Nsp2) coding region and ORF5 ranged from 96.4 to 100 % and 83.2 to 100 %, respectively. All of the 34 Vietnamese HP-PRRSV strains showed two discontinuous 30-amino-acid deletions in the Nsp2 coding region as a genetic marker of prototypic Chinese HP-PRRSV. The amino acid arginine (R) was present at positions 13 and 151 in ORF5 in 29 out of 34 Vietnamese HP-PRRSV isolates, as well as in the prototypic Chinese HP-PRRSV. Sequence analysis of the ORF5 genes of all Vietnamese HP-PRRSVs revealed six subgroups: Viet 1 to 4, JAX1-like, and VR-2332-like. Nucleotide and amino acid sequence analysis of 34 Vietnamese HP-PRRSV isolates from 2013-2014 indicated that Vietnamese HP-PRRSV has undergone rapid evolutionary changes in recent years when compared with Vietnamese HP-PRRSV isolated before 2012.
authors.affiliation: Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, 151-742, Republic of Korea.|Faculty of Animal Husbandry and Veterinary Medicine, Nonglam University, Thu Duc District, Ho Chi Minh, Vietnam.
authors.forename: Duy Tien
authors.initials: DT
authors.lastname: Do
authors.affiliation: Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, 151-742, Republic of Korea.
authors.forename: Changhoon
authors.initials: C
authors.lastname: Park
authors.affiliation: Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, 151-742, Republic of Korea.
authors.forename: Kyuhyung
authors.initials: K
authors.lastname: Choi
authors.affiliation: Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, 151-742, Republic of Korea.
authors.forename: Jiwoon
authors.initials: J
authors.lastname: Jeong
authors.affiliation: Faculty of Animal Husbandry and Veterinary Medicine, Nonglam University, Thu Duc District, Ho Chi Minh, Vietnam.
authors.forename: Toan Tat
authors.initials: TT
authors.lastname: Nguyen
authors.affiliation: Faculty of Animal Husbandry and Veterinary Medicine, Nonglam University, Thu Duc District, Ho Chi Minh, Vietnam.
authors.forename: Dung Thi Hanh
authors.initials: DT
authors.lastname: Le
authors.affiliation: Faculty of Animal Husbandry and Veterinary Medicine, Nonglam University, Thu Duc District, Ho Chi Minh, Vietnam.
authors.forename: Khoa Minh
authors.initials: KM
authors.lastname: Vo
authors.affiliation: Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, 151-742, Republic of Korea. swine@snu.ac.kr.
authors.forename: Chanhee
authors.initials: C
authors.lastname: Chae
mesh_terms: D000818:Animals; D001120:Arginine / Q000235:genetics; D016000:Cluster Analysis; D019143:Evolution, Molecular; D005819:Genetic Markers; D008969:Molecular Sequence Data; D010802:Phylogeny; D019318:Porcine Reproductive and Respiratory Syndrome / Q000821:virology*; D019316:Porcine respiratory and reproductive syndrome virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D012367:RNA, Viral / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion; D017385:Sequence Homology; D013552:Swine; D014744:Vietnam; D014759:Viral Envelope Proteins / Q000235:genetics*; D017361:Viral Nonstructural Proteins / Q000235:genetics*
chemical_list: D005819:Genetic Markers; D012367:RNA, Viral; D014759:Viral Envelope Proteins; D017361:Viral Nonstructural Proteins; C114789:glycoprotein 5, PRRSV; D001120:Arginine