title: Altering Tropism of rAAV by Directed Evolution.
abstract: Directed evolution represents an attractive approach to derive AAV capsid variants capable of selectively infect specific tissue or cell targets. It involves the generation of an initial library of high complexity followed by cycles of selection during which the library is progressively enriched for target-specific variants. Each selection cycle consists of the following: reconstitution of complete AAV genomes within plasmid molecules; production of virions for which each particular capsid variant is matched with the particular capsid gene encoding it; recovery of capsid gene sequences from target tissue after systemic administration. Prevalent variants are then analyzed and evaluated.
authors.affiliation: Department of Pediatrics, College of Medicine, University of Florida, 2033 Mowry Road, CGRC 235, Gainesville, FL, 32610, USA.
authors.forename: Damien
authors.initials: D
authors.lastname: Marsic
authors.affiliation: Department of Pediatrics, College of Medicine, University of Florida, 2033 Mowry Road, CGRC 235, Gainesville, FL, 32610, USA. szlt@ufl.edu.
authors.forename: Sergei
authors.initials: S
authors.lastname: Zolotukhin
mesh_terms: D000818:Animals; D000229:Dependovirus / Q000235:genetics / Q000502:physiology*; D015723:Gene Library; D005818:Genetic Engineering; D016679:Genome, Viral; D057809:HEK293 Cells; D006801:Humans; D051379:Mice; D010957:Plasmids / Q000235:genetics*; D056189:Viral Tropism; D014771:Virion / Q000235:genetics*
keywords: AAV; Capsid library; Directed evolution; Gene therapy; In vivo selection