title: Compartment-specific expression of natural killer cell markers in renal transplantation: immune profile in acute rejection.
abstract: Natural killer (NK) cells have been implicated in graft dysfunction. Here, we formulated hypothesis that distinct patterns of expression NK cells markers correlated with acute rejection in kidney transplantation. Therefore, we studied the pattern of NK cell markers CD56, CD57, and CD16 in different compartments of biopsies obtained from recipients diagnosed with acute graft rejection, with or without donor-specific antibodies (DSA). DSA-negative biopsies-from patients with acute T-cell mediated rejection (aTCMR) had an increased expression of CD56+ and CD57+ cells (P = 0.004 and P = 0.001) in the interstitial compartment in comparison with DSA-positive biopsies from patients acute antibody-mediated rejection (aABMR) with (aABMR C4d+) and without C4d deposition (aABMR C4d-). CD16+ cells was increased (P = 0.03) in the glomerular compartment in DSA-positive biopsies. We assume that CD16+ expression and antibody-dependent cellular cytotoxicity (ADCC) in microvascular injury can be associated with aABMR. IFN-γ release from cytoplasmic granules of NK cell could be associated with aTCMR. Our findings suggest that NK cells need to be carefully evaluated because variations in NK cell marker expression might imply the involvement of different immune system pathways in graft rejection.
authors.affiliation: Department of Pathology, Botucatu Medical School, State University of São Paulo, São Paulo, Brazil.|Department of Pathology, Faculty of Medicine, University of São Paulo, São Paulo, Brazil.
authors.forename: Daniela Cristina
authors.initials: DC
authors.lastname: Dos Santos
authors.affiliation: Department of Immunology, Institute of Biomedical Sciences, University of São Paulo State, São Paulo, Brazil.
authors.forename: Erika Fernandes
authors.initials: EF
authors.lastname: Campos
authors.affiliation: Department of Immunology, Institute of Biomedical Sciences, University of São Paulo State, São Paulo, Brazil.
authors.forename: Niels Olsen
authors.initials: NO
authors.lastname: Saraiva Câmara
authors.affiliation: Department of Pathology, Faculty of Medicine, University of São Paulo, São Paulo, Brazil.
authors.forename: Daisa Silva Ribeiro
authors.initials: DS
authors.lastname: David
authors.affiliation: Department of Pathology, Faculty of Medicine, University of São Paulo, São Paulo, Brazil.
authors.forename: Denise Maria Avancini Costa
authors.initials: DM
authors.lastname: Malheiros
mesh_terms: D000293:Adolescent; D000328:Adult; D000906:Antibodies / Q000276:immunology; D001706:Biopsy; D019002:CD56 Antigen / Q000378:metabolism; D018959:CD57 Antigens / Q000378:metabolism; D003594:Cytoplasmic Granules / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation; D006084:Graft Rejection; D006085:Graft Survival; D006680:HLA Antigens / Q000276:immunology; D006801:Humans; D007107:Immune System; D007150:Immunohistochemistry; D007371:Interferon-gamma / Q000378:metabolism; D016030:Kidney Transplantation; D007694:Killer Cells, Natural / Q000166:cytology*; D008297:Male; D008856:Microscopy, Fluorescence; D008875:Middle Aged; D017452:Receptors, IgG / Q000378:metabolism; D013601:T-Lymphocytes / Q000166:cytology; D055815:Young Adult
chemical_list: D000906:Antibodies; D019002:CD56 Antigen; D018959:CD57 Antigens; D006680:HLA Antigens; C573415:IFNG protein, human; C522918:NCAM1 protein, human; D017452:Receptors, IgG; D007371:Interferon-gamma
keywords: acute T-cell mediated rejection; acute antibody-mediated rejection; donor-specific antibodies; natural killer cells; renal allograft pathology