title: Crystallographic analysis of a cupin superfamily enzyme from Microcystis aeruginosa involved in aeruginosin biosynthesis.
abstract: Aeruginosins are a class of cyanobacteria-derived bioactive linear tetrapeptides composed of nonproteinogenic amino-acid residues, such as the 2-carboxy-6-hydroxyoctahydroindole (Choi) moiety, which is the hallmark of aeruginosin. The biosynthetic pathway of the Choi moiety remains elusive. Previous studies have suggested that AerE, a protein that possesses two cupin domains, participates in the biosynthesis of the Choi moiety. In this study, recombinant AerE from Microcystis aeruginosa, which was overexpressed in Escherichia coli and purified by Ni(2+)-chelating affinity and gel-filtration chromatography, was successfully crystallized and X-ray diffraction analysis was performed. The crystal belonged to the orthorhombic space group P212121, with unit-cell parameters a = 34.770, b = 62.133, c = 87.401 Å. The diffraction data from the crystal were scaled to a maximum resolution of 1.60 Å. The calculated Matthews coefficient of the crystal is 1.93 Å(3) Da(-1), suggesting that there is one molecule in the asymmetric unit.
authors.affiliation: Key Laboratory of Applied Marine Biotechnology of Ministry of Education, School of Marine Sciences, Ningbo University, Ningbo, Zhejiang 315211, People's Republic of China.
authors.forename: Xiaoting
authors.initials: X
authors.lastname: Qiu
authors.affiliation: Key Laboratory of Applied Marine Biotechnology of Ministry of Education, School of Marine Sciences, Ningbo University, Ningbo, Zhejiang 315211, People's Republic of China.
authors.forename: Cui
authors.initials: C
authors.lastname: Xu
authors.affiliation: Key Laboratory of Applied Marine Biotechnology of Ministry of Education, School of Marine Sciences, Ningbo University, Ningbo, Zhejiang 315211, People's Republic of China.
authors.forename: Xu
authors.initials: X
authors.lastname: Chen
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry*; D053898:Biosynthetic Pathways; D002850:Chromatography, Gel; D003460:Crystallization; D018360:Crystallography, X-Ray; D007211:Indoles / Q000378:metabolism*; D046931:Microcystis / Q000201:enzymology*; D008969:Molecular Sequence Data; D005810:Multigene Family; D016415:Sequence Alignment
chemical_list: D001426:Bacterial Proteins; D007211:Indoles
keywords: 2-carboxy-6-hydroxyoctahydroindole moiety; AerE; aeruginosin; cupin domain