title: Microfluidic Impedimetric Cell Regeneration Assay to Monitor the Enhanced Cytotoxic Effect of Nanomaterial Perfusion.
abstract: In the last decade, the application of nanomaterials (NMs) in technical products and biomedicine has become a rapidly increasing market trend. As the safety and efficacy of NMs are of utmost importance, new methods are needed to study the dynamic interactions of NMs at the nano-biointerface. However, evaluation of NMs based on standard and static cell culture end-point detection methods does not provide information on the dynamics of living biological systems, which is crucial for the understanding of physiological responses. To bridge this technological gap, we here present a microfluidic cell culture system containing embedded impedance microsensors to continuously and non-invasively monitor the effects of NMs on adherent cells under varying flow conditions. As a model, the impact of silica NMs on the vitality and regenerative capacity of human lung cells after acute and chronic exposure scenarios was studied over an 18-h period following a four-hour NM treatment. Results of the study demonstrated that the developed system is applicable to reliably analyze the consequences of dynamic NM exposure to physiological cell barriers in both nanotoxicology and nanomedicine.
authors.affiliation: BioSensor Technologies, AIT Austrian Institute of Technology GmbH, 1190 Vienna, Austria. mario.rothbauer@gmail.com.
authors.forename: Mario
authors.initials: M
authors.lastname: Rothbauer
authors.affiliation: BioSensor Technologies, AIT Austrian Institute of Technology GmbH, 1190 Vienna, Austria. ipraisler@gmail.com.
authors.forename: Irene
authors.initials: I
authors.lastname: Praisler
authors.affiliation: Molecular and Cellular Oncology, ENT/University Medical Center Mainz, 55116 Mainz, Germany. docter@uni-mainz.de.
authors.forename: Dominic
authors.initials: D
authors.lastname: Docter
authors.affiliation: Molecular and Cellular Oncology, ENT/University Medical Center Mainz, 55116 Mainz, Germany. rstauber@uni-mainz.de.
authors.forename: Roland H
authors.initials: RH
authors.lastname: Stauber
authors.affiliation: BioSensor Technologies, AIT Austrian Institute of Technology GmbH, 1190 Vienna, Austria. peter.ertl@ait.ac.at.
authors.forename: Peter
authors.initials: P
authors.lastname: Ertl
mesh_terms: D018929:Cell Culture Techniques / Q000295:instrumentation*; D045744:Cell Line, Tumor; D017097:Electric Impedance; D006801:Humans; D056656:Lab-On-A-Chip Devices; D008168:Lung / Q000166:cytology* / Q000187:drug effects* / Q000473:pathology / Q000502:physiology; D049329:Nanostructures / Q000633:toxicity*; D010477:Perfusion / Q000295:instrumentation; D012038:Regeneration; D012822:Silicon Dioxide / Q000633:toxicity*
chemical_list: D012822:Silicon Dioxide
keywords: biosensor; cell chip; impedance; lab-on-a-chip; lung cancer; nanoparticle; nanotoxicology