title: The effect of induced hypertension on neurological outcome in forebrain ischaemia model in rats.
abstract: BACKGROUND
  The present study investigated the effects of induced hypertension on hippocampal cell death after forebrain ischaemia in rats.
  
  
  METHODS
  In this study, forebrain ischaemia was induced in 20 Sprague-Dawley rats by clamping the bilateral common carotid arteries to induce systemic hypotension for 8min. All rats then underwent reperfusion during which the induced hypertension group (n=10) received intermittent intravenous injections of phenylephrine (5μg) to maintain their mean arterial blood pressure at 20mmHg above baseline for 10min and the control group (n=10) did not receive any treatment. In both groups, the numbers of viable and apoptotic neuronal cells in the cornu ammonis 1 (CA1) area of the hippocampus were evaluated 7 days after the induction of ischaemia.
  
  
  RESULTS
  The mean percentage of viable neuronal cells was higher in the induced hypertension group than in the control group (35% vs. 26%, respectively; p=0.004), but there was no significant difference in the proportion of apoptotic neuronal cells between the groups (57% vs. 43%, respectively; p=0.165).
  
  
  CONCLUSIONS
  Induced hypertension significantly attenuated necrotic cell death in the hippocampal CA1 area, but apoptotic cell death was not affected.
authors.affiliation: Department of Anesthesiology and Pain Medicine, Seoul National University Hospital, Seoul, Republic of Korea.
authors.forename: Yoo Sun
authors.initials: YS
authors.lastname: Jung
authors.affiliation: Department of Anesthesiology and Pain Medicine, Seoul Metropolitan Government Seoul National University, Boramae Medical Center, Seoul, Republic of Korea.
authors.forename: Ye-Reum
authors.initials: YR
authors.lastname: Han
authors.affiliation: Department of Anesthesiology and Pain Medicine, College of Medicine, Inha University, Incheon, Republic of Korea.
authors.forename: Byung-Gun
authors.initials: BG
authors.lastname: Kim
authors.affiliation: Department of Anesthesiology and Pain Medicine, Seoul National University Bundang Hospital, Seoul, Republic of Korea.
authors.forename: Ah-Young
authors.initials: AY
authors.lastname: Oh
authors.affiliation: Department of Anesthesiology and Pain Medicine, Seoul National University Hospital, Seoul, Republic of Korea.
authors.forename: Hee-Pyoung
authors.initials: HP
authors.lastname: Park
authors.affiliation: Department of Anesthesiology and Pain Medicine, Seoul National University Bundang Hospital, Seoul, Republic of Korea.
authors.forename: Jung-Won
authors.initials: JW
authors.lastname: Hwang
authors.affiliation: Department of Anesthesiology and Pain Medicine, Seoul National University Hospital, Seoul, Republic of Korea.
authors.forename: Young-Jin
authors.initials: YJ
authors.lastname: Lim
authors.affiliation: Department of Anesthesiology and Pain Medicine, Seoul National University Bundang Hospital, Seoul, Republic of Korea. Electronic address: ytjeon@snubh.org.
authors.forename: Young-Tae
authors.initials: YT
authors.lastname: Jeon
mesh_terms: D000818:Animals; D017209:Apoptosis; D002545:Brain Ischemia / Q000473:pathology*; D056547:CA1 Region, Hippocampal / Q000187:drug effects / Q000473:pathology*; D004195:Disease Models, Animal; D006973:Hypertension / Q000473:pathology*; D002546:Ischemic Attack, Transient / Q000473:pathology*; D009474:Neurons / Q000187:drug effects / Q000473:pathology*; D018696:Neuroprotective Agents / Q000494:pharmacology*; D018384:Oxidative Stress / Q000502:physiology; D016548:Prosencephalon / Q000187:drug effects / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D018696:Neuroprotective Agents
keywords: Apoptosis; Brain ischaemia; Hypertension; Necrosis; Phenylephrine