title: Structural analysis and knock-out of a Burkholderia pseudomallei homolog of the eukaryotic transcription coactivator PC4.
abstract: Homologs of the eukaryotic transcription coactivator PC4, which also functions in DNA repair and oxidative stress, were recently identified in prokaryotes. Crystallographic analysis of BPSL1147, a putative homolog from the pathogen Burkholderia pseudomallei K96243, reveals a highly conserved core structure and suggests a nucleic acid binding mode similar to that of PC4. Knock-out and complementation experiments do not reveal distinguishing phenotypes under normal growth conditions or in the presence of H2O2, arguing against a critical role in repair or the oxidative stress response of Burkholderia. These results may reflect redundancy or point at a bacteriophage origin of Burkholderia PC4 homologs.
authors.affiliation: Department of Molecular Structural Biology, Institute for Biochemistry, University of Greifswald, Felix-Hausdorff-Strasse 4, D-17487 Greifswald, Germany. Electronic address: sebastiaan.werten@uni-greifswald.de.
authors.forename: Sebastiaan
authors.initials: S
authors.lastname: Werten
authors.affiliation: Friedrich Loeffler Institute for Medical Microbiology, Martin-Luther-Strasse 6, D-17475 Greifswald, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Kohler
authors.affiliation: Department of Molecular Structural Biology, Institute for Biochemistry, University of Greifswald, Felix-Hausdorff-Strasse 4, D-17487 Greifswald, Germany.
authors.forename: Niklas Johannes
authors.initials: NJ
authors.lastname: Bayer
authors.affiliation: Friedrich Loeffler Institute for Medical Microbiology, Martin-Luther-Strasse 6, D-17475 Greifswald, Germany.
authors.forename: Ivo
authors.initials: I
authors.lastname: Steinmetz
authors.affiliation: Department of Molecular Structural Biology, Institute for Biochemistry, University of Greifswald, Felix-Hausdorff-Strasse 4, D-17487 Greifswald, Germany.
authors.forename: Winfried
authors.initials: W
authors.lastname: Hinrichs
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D001665:Binding Sites; D016957:Burkholderia pseudomallei / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D018384:Oxidative Stress; D011485:Protein Binding; D014157:Transcription Factors / Q000737:chemistry* / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D014157:Transcription Factors
keywords: Bacteriophage; DNA replication, recombination and repair; Oxidative stress; Positive cofactor 4; Transcription