title: Soluble purified recombinant C2ORF40 protein inhibits esophageal cancer cell proliferation by inducing cell cycle G1phase block.
abstract: Chromosome 2 open reading frame 40 (C2ORF40) plays a significant role in numerous processes, including cell differentiation, senescence, apoptosis, inflammation and neuroendocrine hormone regulation. Moreover, C2ORF40 is a candidate tumor suppressor gene in a variety of tumors, and is closely associated with prognosis. Bioinformatics analysis has indicated that pro-C2ORF40 is a secreted protein with a signal peptide. Secreted C2ORF40 protein (sC2ORF40) exists in cancer cell medium. However, thus far, the exact biological function of sC2ORF40 in carcinogenesis has not been thoroughly researched. In the present study, the signal peptide sequence of the C2ORF40 complementary DNA was initially cut off to produce secreted recombinant human C2ORF40 protein (rhC2ORF40). The soluble rhC2ORF40 was expressed, purified and examined for tumor-suppressing function for the first time. The results revealed that the soluble purified rhC2ORF40 protein was concentrated with a purity of >95%. Furthermore, the rhC2ORF40 inhibited esophageal cancer cell proliferationin vitro(P<0.05) and caused cell cycle G1phase block, as determined by flow cytometric analysis (P<0.05). Overall, the soluble rhC2ORF40 protein with high purity and biological activity was obtained, which suppressed esophageal cancer cells proliferation by inducing cell cycle G1phase blockin vitro. Therefore, the soluble rhC2ORF40 protein could be potential biological therapy drug for esophageal carcinoma.
authors.affiliation: Department of Oncology, Zhengzhou University People's Hospital, Zhengzhou, Henan 450003, P.R. China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Oncology, Zhengzhou University People's Hospital, Zhengzhou, Henan 450003, P.R. China.
authors.forename: Linwei
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Oncology, Zhengzhou University People's Hospital, Zhengzhou, Henan 450003, P.R. China.
authors.forename: Wenyu
authors.initials: W
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Molecular Oncology and Department of Etiology and Carcinogenesis, Cancer Institute and Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, P.R. China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Oncology, Zhengzhou University People's Hospital, Zhengzhou, Henan 450003, P.R. China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Molecular Oncology and Department of Etiology and Carcinogenesis, Cancer Institute and Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, P.R. China.
authors.forename: Shixin
authors.initials: S
authors.lastname: Lu
keywords: biological therapy; cell proliferation; purification; rhC2ORF40