title: Silencing of a Germin-Like Protein Gene (CchGLP) in Geminivirus-Resistant Pepper (Capsicum chinense Jacq.) BG-3821 Increases Susceptibility to Single and Mixed Infections by Geminiviruses PHYVV and PepGMV.
abstract: Germin-like proteins (GLPs) are encoded by a family of genes found in all plants, and in terms of function, the GLPs are implicated in the response of plants to biotic and abiotic stresses. CchGLP is a gene encoding a GLP identified in a geminivirus-resistant Capsicum chinense Jacq accession named BG-3821, and it is important in geminivirus resistance when transferred to susceptible tobacco in transgenic experiments. To characterize the role of this GLP in geminivirus resistance in the original accession from which this gene was identified, this work aimed at demonstrating the possible role of CchGLP in resistance to geminiviruses in Capsicum chinense Jacq. BG-3821. Virus-induced gene silencing studies using a geminiviral vector based in PHYVV component A, displaying that silencing of CchGLP in accession BG-3821, increased susceptibility to geminivirus single and mixed infections. These results suggested that CchGLP is an important factor for geminivirus resistance in C. chinense BG-3821 accession.
authors.affiliation: C.A. Ingeniería de Biosistemas, Facultad de Ingeniería-Campus Amazcala, Carretera a Chichimequillas, Km. 1, S/N, El Marques, Queretaro C.P. 76229, Mexico. lauralyo@yahoo.com.mx.
authors.forename: Laura
authors.initials: L
authors.lastname: Mejía-Teniente
authors.affiliation: Instituto Tecnológico de Roque, Departamento de Ingeniería en Industrias Alimentarias, Km. 8 Carr. Celaya-J. Rosas, Roque, Celaya, Gto C.P. 38110, Mexico. ahuitzolt_jjr@yahoo.com.mx.
authors.forename: Ahuizolt de Jesús
authors.initials: Ade J
authors.lastname: Joaquin-Ramos
authors.affiliation: C.A. Ingeniería de Biosistemas, Facultad de Ingeniería-Campus Amazcala, Carretera a Chichimequillas, Km. 1, S/N, El Marques, Queretaro C.P. 76229, Mexico. torresirineo@gmail.com.
authors.forename: Irineo
authors.initials: I
authors.lastname: Torres-Pacheco
authors.affiliation: Departamento de Ingeniería Genética, Centro de Investigación y de Estudios Avanzados (CINVESTAV)-Unidad Irapuato, Carretera Irapuato-Leon, Km 9.6, Libramiento norte, Irapuato, Guanajuato A.P. 629, Mexico. rrivera@ira.cinvestav.mx.
authors.forename: Rafael F
authors.initials: RF
authors.lastname: Rivera-Bustamante
authors.affiliation: Departamento de Ingeniería Bioquímica, Instituto Tecnológico de Celaya, Ave. Tecnológico y A, Garcia-Cubas, S/N, Col. FOVISSSTE, Celaya, Gto A.P. 57, Mexico. lorenzogo@yahoo.com.
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Guevara-Olvera
authors.affiliation: C.A. Ingeniería de Biosistemas, Facultad de Ingeniería-Campus Amazcala, Carretera a Chichimequillas, Km. 1, S/N, El Marques, Queretaro C.P. 76229, Mexico. ricog@uaq.mx.
authors.forename: Enrique
authors.initials: E
authors.lastname: Rico-García
authors.affiliation: C.A. Ingeniería de Biosistemas, Facultad de Ingeniería-Campus Amazcala, Carretera a Chichimequillas, Km. 1, S/N, El Marques, Queretaro C.P. 76229, Mexico. ramonggg66@gmail.com.
authors.forename: Ramon G
authors.initials: RG
authors.lastname: Guevara-Gonzalez
mesh_terms: D002212:Capsicum / Q000235:genetics / Q000276:immunology* / Q000821:virology*; D060085:Coinfection / Q000276:immunology / Q000821:virology; D060467:Disease Resistance; D017791:Geminiviridae / Q000254:growth & development* / Q000276:immunology*; D020868:Gene Silencing; D010935:Plant Diseases / Q000235:genetics / Q000276:immunology / Q000821:virology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D010940:Plant Proteins
keywords: Germin-like proteins; J0101; Mn-SOD; VIGS; plant-virus interaction; resistance; transient expression