title: Oxidative Stress Attenuates Lipid Synthesis and Increases Mitochondrial Fatty Acid Oxidation in Hepatoma Cells Infected with Hepatitis C Virus.
abstract: Cytopathic effects are currently believed to contribute to hepatitis C virus (HCV)-induced liver injury and are readily observed in Huh7.5 cells infected with the JFH-1 HCV strain, manifesting as apoptosis highly correlated with growth arrest. Reactive oxygen species, which are induced by HCV infection, have recently emerged as activators of AMP-activated protein kinase. The net effect is ATP conservation via on/off switching of metabolic pathways that produce/consume ATP. Depending on the scenario, this can have either pro-survival or pro-apoptotic effects. We demonstrate reactive oxygen species-mediated activation of AMP-activated kinase in Huh7.5 cells during HCV (JFH-1)-induced growth arrest. Metabolic labeling experiments provided direct evidence that lipid synthesis is attenuated, and β-oxidation is enhanced in these cells. A striking increase in nuclear peroxisome proliferator-activated receptor α, which plays a dominant role in the expression of β-oxidation genes after ligand-induced activation, was also observed, and we provide evidence that peroxisome proliferator-activated receptor α is constitutively activated in these cells. The combination of attenuated lipid synthesis and enhanced β-oxidation is not conducive to lipid accumulation, yet cellular lipids still accumulated during this stage of infection. Notably, the serum in the culture media was the only available source for polyunsaturated fatty acids, which were elevated (2-fold) in the infected cells, implicating altered lipid import/export pathways in these cells. This study also provided the first in vivo evidence for enhanced β-oxidation during HCV infection because HCV-infected SCID/Alb-uPA mice accumulated higher plasma ketones while fasting than did control mice. Overall, this study highlights the reprogramming of hepatocellular lipid metabolism and bioenergetics during HCV infection, which are predicted to impact both the HCV life cycle and pathogenesis.
authors.affiliation: From the Departments of Surgery,; Li Ka Shing Institute of Virology, University of Alberta, Edmonton, Alberta T6G 2E1, Canada. Electronic address: donnad@ualberta.ca.
authors.forename: Donna N
authors.initials: DN
authors.lastname: Douglas
authors.affiliation: From the Departments of Surgery,; Li Ka Shing Institute of Virology, University of Alberta, Edmonton, Alberta T6G 2E1, Canada.
authors.forename: Christopher Hao
authors.initials: CH
authors.lastname: Pu
authors.affiliation: From the Departments of Surgery,; Li Ka Shing Institute of Virology, University of Alberta, Edmonton, Alberta T6G 2E1, Canada.
authors.forename: Jamie T
authors.initials: JT
authors.lastname: Lewis
authors.affiliation: Medical Microbiology and Immunology.
authors.forename: Rakesh
authors.initials: R
authors.lastname: Bhat
authors.affiliation: Medical Microbiology and Immunology.
authors.forename: Anwar
authors.initials: A
authors.lastname: Anwar-Mohamed
authors.affiliation: Li Ka Shing Institute of Virology, University of Alberta, Edmonton, Alberta T6G 2E1, Canada; Medical Microbiology and Immunology.
authors.forename: Michael
authors.initials: M
authors.lastname: Logan
authors.affiliation: KMT Hepatech Inc., and.
authors.forename: Garry
authors.initials: G
authors.lastname: Lund
authors.affiliation: Li Ka Shing Institute of Virology, University of Alberta, Edmonton, Alberta T6G 2E1, Canada; Medical Microbiology and Immunology.
authors.forename: William R
authors.initials: WR
authors.lastname: Addison
authors.affiliation: Cell Biology,; Pediatrics.
authors.forename: Richard
authors.initials: R
authors.lastname: Lehner
authors.affiliation: From the Departments of Surgery,; Li Ka Shing Institute of Virology, University of Alberta, Edmonton, Alberta T6G 2E1, Canada.
authors.forename: Norman M
authors.initials: NM
authors.lastname: Kneteman
mesh_terms: D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D005227:Fatty Acids / Q000378:metabolism*; D016174:Hepacivirus / Q000502:physiology*; D006526:Hepatitis C / Q000378:metabolism* / Q000821:virology; D006801:Humans; D008055:Lipids / Q000096:biosynthesis*; D008099:Liver / Q000378:metabolism; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D051379:Mice; D016513:Mice, SCID; D008928:Mitochondria / Q000235:genetics / Q000378:metabolism*; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D047493:PPAR alpha / Q000235:genetics / Q000378:metabolism
chemical_list: D005227:Fatty Acids; D008055:Lipids; D047493:PPAR alpha
keywords: AMP-activated kinase (AMPK); hepatitis C virus (HCV); lipogenesis; oxidative stress; β-oxidation