title: Induction of Spermatogenesis by Bone Marrow-derived Mesenchymal Stem Cells in Busulfan-induced Azoospermia in Hamster.
abstract: BACKGROUND
  Bone marrow-derived mesenchymal stem cells (BM-MSCs) have potential of differentiation and they secrete anti-inflammatory cytokines and growth factors which make them appropriate for cell therapy.
  
  
  OBJECTIVE
  Were to evaluate the healing effect of BM-MSCs transplantation on germinal cells of busulfan-induced azoospermic hamsters.
  
  
  METHODS
  In the present experimental case control study, BM-MSCs were isolated from bone marrow of donor albino hamsters. Five mature male recipient hamsters received two doses of 10 mg/kg of busulfan with 21 days interval to stop endogenous spermatogenesis. After induction of azoospermia, right testis of hamsters was injected with 10(6) BM-MSCs via efferent duct and the left one remained as azoospermia control testis. Five normal mature hamsters were selected as normal intact control. After 35 days, testes and epididymis of three groups were removed for histological evaluation.
  
  
  RESULTS
  Histomorphological analyses of BM-MSCs treated testes and epididymis showed the epithelial tissue of seminiferous tubules had normal morphology and spermatozoa were present in epididymis tubes. Spermatogenesis was observed in most cell-treated seminiferous tubules. The untreated seminiferous tubules were empty.
  
  
  CONCLUSIONS
  Transplanted BM-MSCs could successfully induce spermatogenesis in seminiferous tubules of azoospermic hamster. Therefore, BM-MSCs can be an attractive candidate in cell transplantation of azoospermia.
authors.affiliation: Stem Cell and Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Amin
authors.initials: A
authors.lastname: Tamadon
authors.affiliation: Stem Cell and Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Davood
authors.initials: D
authors.lastname: Mehrabani
authors.affiliation: Department of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
authors.forename: Farhad
authors.initials: F
authors.lastname: Rahmanifar
authors.affiliation: Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
authors.forename: Alireza Raayat
authors.initials: AR
authors.lastname: Jahromi
authors.affiliation: DVM graduated, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
authors.forename: Mohadeseh
authors.initials: M
authors.lastname: Panahi
authors.affiliation: Stem Cell and Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Shahrokh
authors.initials: S
authors.lastname: Zare
authors.affiliation: Stem Cell and Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Khodabandeh
authors.affiliation: Stem Cell and Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Iman Razeghian
authors.initials: IR
authors.lastname: Jahromi
authors.affiliation: Stem Cell and Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran ; Department of Pharmacology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Nader
authors.initials: N
authors.lastname: Tanideh
authors.affiliation: Stem Cell and Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran ; Department of Human Genetic, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Dianatpour
authors.affiliation: Hematology and Bone Marrow Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Mani
authors.initials: M
authors.lastname: Ramzi
authors.affiliation: Laboratory Animal Center, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Omid
authors.initials: O
authors.lastname: Koohi-Hoseinabadi
keywords: Azoospermia; Bone marrow; Busulfan; Cell therapy; Hamster; Mesenchymal stem cell