title: Analysis of an "off-ladder" allele at the Penta D short tandem repeat locus.
abstract: Kinship testing of a father and his son from Guangxi, China, the location of the Zhuang minority people, was performed using the PowerPlex® 18D System with a short tandem repeat typing kit. The results indicated that both the father and his son had an off-ladder allele at the Penta D locus, with a genetic size larger than that of the maximal standard allelic ladder. To further identify this locus, monogenic amplification, gene cloning, and genetic sequencing were performed. Sequencing analysis demonstrated that the fragment size of the Penta D-OL locus was 469 bp and the core sequence was [AAAGA]21, also called Penta D-21. The rare Penta D-21 allele was found to be distributed among the Zhuang population from the Guangxi Zhuang Autonomous Region of China; therefore, this study improved the range of DNA data available for this locus and enhanced our ability for individual identification of gene loci.
authors.affiliation: Clinical Laboratory Center, Traditional Chinese Medical Hospital of Langfang City, Langfang, Hebei, China.
authors.forename: Y L
authors.initials: YL
authors.lastname: Yang
authors.affiliation: Department of Internal Medicine, Traditional Chinese Medical Hospital of Langfang City, Langfang, Hebei, China.
authors.forename: J G
authors.initials: JG
authors.lastname: Wang
authors.affiliation: Clinical Laboratory Center, Traditional Chinese Medical Hospital of Langfang City, Langfang, Hebei, China.
authors.forename: D X
authors.initials: DX
authors.lastname: Wang
authors.affiliation: Clinical Laboratory Center, Traditional Chinese Medical Hospital of Langfang City, Langfang, Hebei, China.
authors.forename: W Y
authors.initials: WY
authors.lastname: Zhang
authors.affiliation: Department of Forensics Laboratory, Nanning Institute of Transfusion Medicine, Nanning, Guangxi, China.
authors.forename: X J
authors.initials: XJ
authors.lastname: Liu
authors.affiliation: Clinical Laboratory Center, Traditional Chinese Medical Hospital of Langfang City, Langfang, Hebei, China.
authors.forename: J
authors.initials: J
authors.lastname: Cao
authors.affiliation: Department of Hematology, Traditional Chinese Medical Hospital of Langfang City, Langfang, Hebei, China shulianyang@126.com.
authors.forename: S L
authors.initials: SL
authors.lastname: Yang
mesh_terms: D000483:Alleles; D002681:China; D003001:Cloning, Molecular; D019075:Electrophoresis, Capillary; D056426:Genetic Loci; D014644:Genetic Variation; D006801:Humans; D018895:Microsatellite Repeats / Q000235:genetics*; D009844:Oligosaccharides; D017422:Sequence Analysis, DNA
chemical_list: D009844:Oligosaccharides; C064764:PENTA