title: Gene Expression Profiles of Main Olfactory Epithelium in Adenylyl Cyclase 3 Knockout Mice.
abstract: Adenylyl Cyclase 3 (AC3) plays an important role in the olfactory sensation-signaling pathway in mice. AC3 deficiency leads to defects in olfaction. However, it is still unknown whether AC3 deficiency affects gene expression or olfactory signal transduction pathways within the main olfactory epithelium (MOE). In this study, gene microarrays were used to screen differentially expressed genes in MOE from AC3 knockout (AC3(-/-)) and wild-type (AC3(+/+)) mice. The differentially expressed genes identified were subjected to bioinformatic analysis and verified by qRT-PCR. Gene expression in the MOE from AC3(-/-) mice was significantly altered, compared to AC3(+/+) mice. Of the 41266 gene probes, 3379 had greater than 2-fold fold change in expression levels between AC3(-/-) and AC3(+/+) mice, accounting for 8% of the total gene probes. Of these genes, 1391 were up regulated, and 1988 were down regulated, including 425 olfactory receptor genes, 99 genes that are specifically expressed in the immature olfactory neurons, 305 genes that are specifically expressed in the mature olfactory neurons, and 155 genes that are involved in epigenetic regulation. Quantitative RT-PCR verification of the differentially expressed epigenetic regulation related genes, olfactory receptors, ion transporter related genes, neuron development and differentiation related genes, lipid metabolism and membrane protein transport etc. related genes showed that P75NTR, Hinfp, Gadd45b, and Tet3 were significantly up-regulated, while Olfr370, Olfr1414, Olfr1208, Golf, Faim2, Tsg101, Mapk10, Actl6b, H2BE, ATF5, Kirrrel2, OMP, Drd2 etc. were significantly down-regulated. In summary, AC3 may play a role in proximal olfactory signaling and play a role in the regulation of differentially expressed genes in mouse MOE.
authors.affiliation: College of Life Science, Hebei University, Baoding 071002, China. zswang@hbu.edu.cn.
authors.forename: Zhenshan
authors.initials: Z
authors.lastname: Wang
authors.affiliation: College of Life Science, Hebei University, Baoding 071002, China. zhou66913@163.com.
authors.forename: Yanfen
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: College of Life Science, Hebei University, Baoding 071002, China. luoyingtao1987@sina.com.
authors.forename: Yingtao
authors.initials: Y
authors.lastname: Luo
authors.affiliation: College of Life Science, Hebei University, Baoding 071002, China. zhangjing_0521@sohu.com.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: College of Life Science, Hebei University, Baoding 071002, China. zypkid1412@sina.com.
authors.forename: Yunpeng
authors.initials: Y
authors.lastname: Zhai
authors.affiliation: College of Life Science, Hebei University, Baoding 071002, China. yangdong87@yahoo.com.
authors.forename: Dong
authors.initials: D
authors.lastname: Yang
authors.affiliation: College of Life Science, Hebei University, Baoding 071002, China. zhangzhe_2005@126.com.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China. liyongchao@genetics.ac.cn.
authors.forename: Yongchao
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Pharmacology, University of Washington, Seattle, WA 98195, USA. dstorm@u.washington.edu.
authors.forename: Daniel R
authors.initials: DR
authors.lastname: Storm
authors.affiliation: Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China. rlma@genetics.ac.cn.
authors.forename: Runlin Z
authors.initials: RZ
authors.lastname: Ma
mesh_terms: D000262:Adenylyl Cyclases / Q000172:deficiency*; D000818:Animals; D017209:Apoptosis / Q000235:genetics; D049109:Cell Proliferation; D019295:Computational Biology / Q000379:methods; D000242:Cyclic AMP / Q000378:metabolism; D044127:Epigenesis, Genetic; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D063990:Gene Ontology; D051379:Mice; D018345:Mice, Knockout; D058977:Molecular Sequence Annotation; D009831:Olfactory Mucosa / Q000378:metabolism*; D018034:Olfactory Receptor Neurons / Q000378:metabolism; D015203:Reproducibility of Results; D015398:Signal Transduction; D059467:Transcriptome
chemical_list: D000242:Cyclic AMP; D000262:Adenylyl Cyclases
keywords: adenylyl cyclase 3; differentially expressed genes; main olfactory epithelium; microarray analysis