title: Pyranocoumarins from Root Extracts of Peucedanum praeruptorum Dunn with Multidrug Resistance Reversal and Anti-Inflammatory Activities.
abstract: In the search for novel herbal-based anticancer agents, we isolated a new angular-type pyranocoumarin, (+)-cis-(3'S,4'S)-3'-angeloyl-4'-tigloylkhellactone (1) along with 12 pyranocoumarins (2-13), two furanocoumarins (14, 15), and a polyacetylene (16) were isolated from the roots of Peucedanum praeruptorum using chromatographic separation methods. The structures of the compounds were determined using spectroscopic analysis with nuclear magnetic resonance (NMR) and high-resolution-electrospray ionization-mass spectrometry (HR-ESI-MS). The multidrug-resistance (MDR) reversal and anti-inflammatory effects of all the isolated compounds were evaluated in human sarcoma MES-SA/Dx5 and lipopolysaccharide (LPS)-induced RAW 264.7 cells. Among the 16 tested compounds, two (2 and 16) downregulated nitric oxide (NO) production and five (1, 7, 8, 11, and 13) inhibited the efflux of drugs by MDR protein, indicating the reversal of MDR. Therefore, these compounds may be potential candidates for the development of effective agents against MDR forms of cancer.
authors.affiliation: KM Convergence Research Division, Korea Institute of Oriental Medicine, Daejeon 34054, Korea. junlee@kiom.re.kr.|Korean Medicine Life Science, University of Science &amp; Technology, Daejeon 34054, Korea. junlee@kiom.re.kr.
authors.forename: Jun
authors.initials: J
authors.lastname: Lee
authors.affiliation: KM Convergence Research Division, Korea Institute of Oriental Medicine, Daejeon 34054, Korea. ojung8384@kiom.re.kr.
authors.forename: You Jin
authors.initials: YJ
authors.lastname: Lee
authors.affiliation: KM Convergence Research Division, Korea Institute of Oriental Medicine, Daejeon 34054, Korea. jinheekim@kiom.re.kr.
authors.forename: Jinhee
authors.initials: J
authors.lastname: Kim
authors.affiliation: KM Convergence Research Division, Korea Institute of Oriental Medicine, Daejeon 34054, Korea. osbang@kiom.re.kr.
authors.forename: Ok-Sun
authors.initials: OS
authors.lastname: Bang
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D019661:Apiaceae / Q000737:chemistry*; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D018432:Drug Resistance, Multiple / Q000187:drug effects; D019008:Drug Resistance, Neoplasm / Q000187:drug effects; D005260:Female; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000166:cytology / Q000187:drug effects; D051379:Mice; D015394:Molecular Structure; D009569:Nitric Oxide / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry; D039681:Pyranocoumarins / Q000737:chemistry / Q000494:pharmacology*; D012509:Sarcoma / Q000188:drug therapy* / Q000473:pathology; D014594:Uterine Neoplasms / Q000188:drug therapy* / Q000473:pathology
chemical_list: D000893:Anti-Inflammatory Agents; D000970:Antineoplastic Agents; D016207:Cytokines; D008070:Lipopolysaccharides; D010936:Plant Extracts; D039681:Pyranocoumarins; D009569:Nitric Oxide
keywords: Peucedanum praeruptorum; Umbelliferae; anti-inflammation; multidrug resistance; pyranocoumarin