title: Characterization of Pseudomonas aeruginosa phage KPP21 belonging to family Podoviridae genus N4-like viruses isolated in Japan.
abstract: Bacteriophages (phages) belonging to the family Podoviridae genus N4-like viruses have been used as therapeutic agent in phage therapy against Pseudomonas aeruginosa infections. P. aeruginosa phage KPP21 was isolated in Japan, and phylogenetically investigated the phages belonging to this viral genus. Morphological and genetic analyses confirmed that phage KPP21 belongs to the family Podoviridae genus N4-like viruses. Moreover, phylogenetic analyses based on putative DNA polymerase and major virion protein showed that P. aeruginosa phages belonging to the genus N4-like viruses are separated into two lineages and that phage KPP21 is in the same clade as phage LUZ7.
authors.affiliation: Department of Microbiology and Infection, Kochi Medical School, Kochi University, Kochi.
authors.forename: Ryu
authors.initials: R
authors.lastname: Shigehisa
authors.affiliation: Department of Veterinary Microbiology, School of Veterinary Medicine, Azabu University, Kanagawa.
authors.forename: Jumpei
authors.initials: J
authors.lastname: Uchiyama
authors.affiliation: Science Research Center, Kochi University, Kochi.
authors.forename: Shin-Ichiro
authors.initials: S
authors.lastname: Kato
authors.affiliation: Department of Veterinary Microbiology, School of Veterinary Medicine, Azabu University, Kanagawa.
authors.forename: Iyo
authors.initials: I
authors.lastname: Takemura-Uchiyama
authors.affiliation: Department of Microbiology and Infection, Kochi Medical School, Kochi University, Kochi.
authors.forename: Kotoe
authors.initials: K
authors.lastname: Yamaguchi
authors.affiliation: Department of Microbiology and Infection, Kochi Medical School, Kochi University, Kochi.
authors.forename: Reina
authors.initials: R
authors.lastname: Miyata
authors.affiliation: Science Research Center, Kochi University, Kochi.
authors.forename: Takako
authors.initials: T
authors.lastname: Ujihara
authors.affiliation: Department of Microbiology, Kitasato University School of Medicine, Kanagawa, Japan.
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Sakaguchi
authors.affiliation: Department of Veterinary Microbiology, School of Veterinary Medicine, Azabu University, Kanagawa.
authors.forename: Noriaki
authors.initials: N
authors.lastname: Okamoto
authors.affiliation: Department of Veterinary Microbiology, School of Veterinary Medicine, Azabu University, Kanagawa.
authors.forename: Hidekatsu
authors.initials: H
authors.lastname: Shimakura
authors.affiliation: Department of Microbiology and Infection, Kochi Medical School, Kochi University, Kochi.
authors.forename: Masanori
authors.initials: M
authors.lastname: Daibata
authors.affiliation: Department of Veterinary Microbiology, School of Veterinary Medicine, Azabu University, Kanagawa.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Sakaguchi
authors.affiliation: Department of Microbiology and Infection, Kochi Medical School, Kochi University, Kochi.
authors.forename: Shigenobu
authors.initials: S
authors.lastname: Matsuzaki
mesh_terms: D001482:Base Composition; D002874:Chromosome Mapping; D004279:DNA, Viral / Q000235:genetics*; D016679:Genome, Viral; D007564:Japan; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D010802:Phylogeny; D017902:Podoviridae / Q000145:classification* / Q000302:isolation & purification / Q000648:ultrastructure; D011552:Pseudomonas Infections / Q000821:virology; D017105:Pseudomonas Phages / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000648:ultrastructure; D011550:Pseudomonas aeruginosa / Q000821:virology*
chemical_list: D004279:DNA, Viral
keywords: Pseudomonas aeruginosa; bacteriophage; phage therapy; phylogeny