title: Optimization for ultrasonic-microwave synergistic extraction of polysaccharides from Cornus officinalis and characterization of polysaccharides.
abstract: Ultrasonic-microwave synergistic extraction (UMSE) of polysaccharides from Cornus officinalis was optimized by response surface methodology (RSM). The effect of four different factors on the yield of C. officinalis polysaccharides (COP) was studied. RSM results showed that the optimal conditions were extraction time of 31.49823 min, microwave power of 99.39769 W, and water-to-raw material ratio of 28.16273. The COP yield was 11.38±0.31% using the modified optimal conditions, which was consistent with the value predicted by the model. The crude COP was purified by DEAE-Cellulose 52 chromatography and Sephadex G-100 chromatography. Five fractions, namely, crude COP, COP-1, COP-2, COP-3, and COP-4, were obtained. Monosaccharide composition analysis revealed that the COP was composed of glucose, arabinose, fucose, xylose, mannose, and rhamnose. Preliminary structural characterizations of COP were conducted by scanning electron microscopy and Fourier transform infrared spectroscopy.
authors.affiliation: School of Life Science and Chemical Engineering, Huaiyin Institute of Technology, Huaian 223003, China; Jiangsu Provincial Engineering Laboratory for Biomass Conversion and Process Integration, Huaiyin Institute of Technology, Huaian 223003, China.
authors.forename: Xiulian
authors.initials: X
authors.lastname: Yin
authors.affiliation: School of Life Science and Chemical Engineering, Huaiyin Institute of Technology, Huaian 223003, China; Jiangsu Provincial Engineering Laboratory for Biomass Conversion and Process Integration, Huaiyin Institute of Technology, Huaian 223003, China; Jiangsu Key Laboratory of Quality Control and Further Processing of Cereals and Oils, Nanjing University of Finance and Economics, 301 Wenyuan Road, Nanjing 210023, China. Electronic address: youqhong@163.com.
authors.forename: Qinghong
authors.initials: Q
authors.lastname: You
authors.affiliation: School of Life Science and Chemical Engineering, Huaiyin Institute of Technology, Huaian 223003, China.
authors.forename: Zhonghai
authors.initials: Z
authors.lastname: Jiang
authors.affiliation: School of Life Science and Chemical Engineering, Huaiyin Institute of Technology, Huaian 223003, China.
authors.forename: Xinghai
authors.initials: X
authors.lastname: Zhou
mesh_terms: D005591:Chemical Fractionation / Q000379:methods*; D029768:Cornus / Q000737:chemistry*; D008872:Microwaves; D008962:Models, Theoretical; D009005:Monosaccharides / Q000032:analysis; D010940:Plant Proteins / Q000032:analysis; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification*; D000069453:Ultrasonic Waves; D014574:Uronic Acids / Q000032:analysis
chemical_list: D009005:Monosaccharides; D010940:Plant Proteins; D011134:Polysaccharides; D014574:Uronic Acids
keywords: Cornus officinalis; The yield of Cornus officinalis polysaccharides; Ultrasonic-microwave synergistic extraction