title: Production and Analysis of Biological Properties of Recombinant Human Apolipoprotein A-I.
abstract: Production of recombinant human apolipoprotein A-I (apoA-I) in E. coli cells is described and its biological properties are compared with those of natural protein. Recombinant apoA-I was isolated as a chimeric polypeptide and then processed to a mature form apoA-I (rapo-I). We studied the ability of the resulting protein to penetrate into hepatocyte nuclei and regulate the rate of DNA biosynthesis in complex with estriol. Penetration of rapoA-I conjugated with FITC into hepatocyte nuclei was demonstrated. rapoA-I-estriol and apoA-I-estriol complexes induced similar increase in DNA biosynthesis rate in isolated hepatocytes, which confi rms functional similarity of the obtained recombinant mature protein (rapoA-I) and native human apoA-I.
authors.affiliation: Laboratory of Medical Biotechnology, Research Institute of Biochemistry, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia. borrelia@mail.ru.
authors.forename: A V
authors.initials: AV
authors.lastname: Ryabchenko
authors.affiliation: Laboratory of Medical Biotechnology, Research Institute of Biochemistry, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia.
authors.forename: M V
authors.initials: MV
authors.lastname: Kotova
authors.affiliation: Laboratory of Medical Biotechnology, Research Institute of Biochemistry, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia.
authors.forename: N V
authors.initials: NV
authors.lastname: Tverdohleb
authors.affiliation: Laboratory of Medical Biotechnology, Research Institute of Biochemistry, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia.
authors.forename: R A
authors.initials: RA
authors.lastname: Knyazev
authors.affiliation: Laboratory of Medical Biotechnology, Research Institute of Biochemistry, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia.
authors.forename: L M
authors.initials: LM
authors.lastname: Polyakov
mesh_terms: D016632:Apolipoprotein A-I / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology*; D002466:Cell Nucleolus / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D002478:Cells, Cultured; D004261:DNA Replication / Q000187:drug effects; D004926:Escherichia coli; D004964:Estriol / Q000494:pharmacology; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D011993:Recombinant Fusion Proteins / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: C505693:APOA1 protein, human; D016632:Apolipoprotein A-I; D005456:Fluorescent Dyes; D011993:Recombinant Fusion Proteins; D004964:Estriol; D016650:Fluorescein-5-isothiocyanate
keywords: DNA biosynthesis; E. coli; estriol; hepatocytes; recombinant apoA-I protein