title: Outbreak of ranavirus infection in sheatfish, Silurus glanis (L.), in Poland.
abstract: Ranavirus was detected in adult sheatfish, with clinical signs, on a Polish fish farm in February. Farm isolates induced a strong cytopathic effect in vitro and were identified by electron microscopy and PCR amplification of the ranavirus specific gene fragment. Restriction analysis with the Acc I enzyme showed that isolated ranaviruses were different from the epizootic heamatopoietic necrosis virus (EHNV). We sequenced a fragment of the major capsid protein (MCP) gene and found that isolates were similar to other strains of ranaviruses, available in GenBank.
authors.forename: E
authors.initials: E
authors.lastname: Borzym
authors.forename: T A
authors.initials: TA
authors.lastname: Karpińska
authors.forename: M
authors.initials: M
authors.lastname: Reichert
mesh_terms: D000818:Animals; D002397:Catfishes; D004266:DNA Virus Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D005393:Fish Diseases / Q000453:epidemiology / Q000821:virology*; D010802:Phylogeny; D011044:Poland / Q000453:epidemiology; D017995:Ranavirus / Q000235:genetics / Q000302:isolation & purification*