title: Dopamine biosensor based on surface functionalized nanostructured nickel oxide platform.
abstract: A dopamine biosensor has been developed using nickel oxide nanoparticles (NPs) and tyrosinase enzyme conjugate. Nickel oxide (NiO) NPs were synthesized by sol-gel method using anionic surfactant, sodium dodecyl sulphate (SDS), as template to control the size of synthesized nanoparticles. The structural and morphological studies of the prepared NPs were carried out using X-ray diffraction (XRD), transmission electron microscopy (TEM) and dynamic light scattering (DLS) techniques. Afterwards, tyrosinase enzyme molecules were adsorbed on NiO NPs surface and enzyme coated NPs were deposited on indium tin oxide (ITO) coated flexible polyethylene terephthalate (PET) substrate by solution casting method. The formation of enzyme-NPs conjugate was investigated by atomic force microscopy (AFM) and Fourier transform infrared spectroscopy (FTIR) techniques and used in selective detection and estimation of neurochemical dopamine by electrochemical method. The fabricated Tyrosinase/NiO/ITO electrode exhibits high sensitivity of 60.2nA/µM in linear detection range (2-100μM) with a detection limit of 1.038μM. The proposed sensor had a response time of 45s, long shelf life (45 days) with good reproducibility and selectivity in presence of interfering substances and was validated with real samples. The tyrosinase enzyme functionalized NiO platform has good bio-sensing efficacy and can be used in detection of other catecholamines and phenolic neurochemicals.
authors.affiliation: Centre for Biomedical Engineering, Indian Institute of Technology Delhi, Hauz Khas, New Delhi 110016, India.
authors.forename: Appan
authors.initials: A
authors.lastname: Roychoudhury
authors.affiliation: Department of Chemical Engineering, Indian Institute of Technology Delhi, Hauz Khas, New Delhi 110016, India.
authors.forename: Suddhasatwa
authors.initials: S
authors.lastname: Basu
authors.affiliation: Centre for Biomedical Engineering, Indian Institute of Technology Delhi, Hauz Khas, New Delhi 110016, India. Electronic address: sandeepjha@cbme.iitd.ac.in.
authors.forename: Sandeep Kumar
authors.initials: SK
authors.lastname: Jha
mesh_terms: D000364:Agaricus / Q000201:enzymology; D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D002417:Cattle; D004298:Dopamine / Q000097:blood*; D055664:Electrochemical Techniques / Q000379:methods*; D004800:Enzymes, Immobilized / Q000737:chemistry; D057230:Limit of Detection; D014442:Monophenol Monooxygenase / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D009532:Nickel / Q000737:chemistry*; D011093:Polyethylene Terephthalates / Q000737:chemistry; D017971:Tin Compounds / Q000737:chemistry
chemical_list: D004800:Enzymes, Immobilized; D011093:Polyethylene Terephthalates; D017971:Tin Compounds; C109984:indium tin oxide; D009532:Nickel; C028007:nickel monoxide; D014442:Monophenol Monooxygenase; D004298:Dopamine
keywords: Dopamine biosensor; Electrochemical sensor; Neurochemical detection; NiO nanoparticles; Tyrosinase