title: Highly sensitive and simultaneous detection of melamine and aflatoxin M1 in milk products by multiplexed planar waveguide fluorescence immunosensor (MPWFI).
abstract: Mycotoxins and industrial chemicals, such as aflatoxin M1 and melamine, now commonly exist in milk and cause potential health risks. This study presents an indirect competitive immunoassay through multiplex planar waveguide fluorescence immunosensor (MPWFI) for rapid, sensitive, and simultaneous detection and quantification of aflatoxin M1 and melamine by applying the principle of immunoreaction and total internal reflect fluorescent. Double-channel standard curves with appropriate logistic correlation (R(2)>0.99) were plotted, respectively. The working ranges (0.073-0.400 ng/mL and 26.38-270.00 ng/mL, respectively) were calculated, as well as the limit of detection (0.045 and 13.37 ng/mL, respectively), when two analytes were simultaneously detected. Both results satisfied the requirements for the maximum amount set by the WHO, which illustrated that the current method was better than some other standard methods. The recovery rates in the actual samples ranged from 85% to 103%, with relative standard deviations between 1.3% and 6.5%, which indicated high accuracy and repeatability.
authors.affiliation: State Key Joint Laboratory of Environmental Simulate & Pollution Control, School of Environment, Tsinghua University, Beijing 10084, China.
authors.forename: Hongli
authors.initials: H
authors.lastname: Guo
authors.affiliation: State Key Joint Laboratory of Environmental Simulate & Pollution Control, School of Environment, Tsinghua University, Beijing 10084, China.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Hebei Institute of Food Quality Supervision Inspection & Research, Shijiazhuang 050091, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: State Key Joint Laboratory of Environmental Simulate & Pollution Control, School of Environment, Tsinghua University, Beijing 10084, China.
authors.forename: Baodong
authors.initials: B
authors.lastname: Song
authors.affiliation: Hebei Institute of Food Quality Supervision Inspection & Research, Shijiazhuang 050091, China.
authors.forename: Jingxuan
authors.initials: J
authors.lastname: Zhang
authors.affiliation: State Key Joint Laboratory of Environmental Simulate & Pollution Control, School of Environment, Tsinghua University, Beijing 10084, China. Electronic address: hanchang@mail.tsinghua.edu.cn.
authors.forename: Hanchang
authors.initials: H
authors.lastname: Shi
mesh_terms: D016607:Aflatoxin M1 / Q000032:analysis*; D000818:Animals; D002417:Cattle; D005453:Fluorescence; D005506:Food Contamination / Q000032:analysis*; D007118:Immunoassay / Q000379:methods*; D008892:Milk / Q000737:chemistry*; D014227:Triazines / Q000032:analysis*
chemical_list: D014227:Triazines; D016607:Aflatoxin M1; C011907:melamine
keywords: Aflatoxin M1; Immunoassay; Melamine; Milk products; Multiplex planar waveguide fluorescence immunosensor