title: Role of nutrients and environmental conditions for the production of dextransucrase from L. mesenteroides KIBGE-IB26.
abstract: The bacterial strains capable of producing dextransucrase enzyme were isolated from different fruits and vegetables sources. In primary screening, five strains were selected on the basis dextransucrase production and among them L. mesenteroides KIBGE- IB26 isolated from bottle gourd (Lagenaria Vulgaris) was selected for further studies. For the enhancement of enzyme production, different physicochemical parameters were optimized. Maximum production of dextransucrase was achieved after 06 hrs using sucrose (20.0 g/l) as a substrate at 25°C. Maximum dextransucrase production was achieved when medium pH was kept 7.5 before sterilization. In addition, medium was also supplemented with CaCl2 and K2HPO4 and maximum enzyme production was achieved at 0.0025 g/dl calcium chloride and 2.0 g/dl K₂HPO₄with enzyme activity of 87 DSU/ml/hr. Production of dextransucrase in shorter period of time makes this strain an attractive candidate for commercial production of dextransucrase.
authors.affiliation: Pharmaceutical Research Centre, Pakistan Council of Scientific & Industrial Research (PCSIR) Laboratories Complex, Karachi, Karachi, Pakistan.
authors.forename: Samina
authors.initials: S
authors.lastname: Iqbal
authors.affiliation: Industrial Biotechnology Section, The Karachi Institute of Biotechnology & Genetic Engineering (KIBGE), University of Karachi, Karachi, Pakistan.
authors.forename: Afsheen
authors.initials: A
authors.lastname: Aman
authors.affiliation: Pharmaceutical Research Centre, Pakistan Council of Scientific & Industrial Research (PCSIR) Laboratories Complex, Karachi, Karachi, Pakistan.
authors.forename: Saeeda
authors.initials: S
authors.lastname: Bano
authors.affiliation: Industrial Biotechnology Section, The Karachi Institute of Biotechnology & Genetic Engineering (KIBGE), University of Karachi, Karachi, Pakistan.
authors.forename: Nadir Naveed
authors.initials: NN
authors.lastname: Sidduiqui
authors.affiliation: Industrial Biotechnology Section, The Karachi Institute of Biotechnology & Genetic Engineering (KIBGE), University of Karachi, Karachi, Pakistan.
authors.forename: Asma
authors.initials: A
authors.lastname: Ansari
authors.affiliation: Industrial Biotechnology Section, The Karachi Institute of Biotechnology & Genetic Engineering (KIBGE), University of Karachi, Karachi, Pakistan.
authors.forename: Shah AliUl
authors.initials: SA
authors.lastname: Qader
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis*; D002122:Calcium Chloride / Q000378:metabolism; D005285:Fermentation; D005964:Glucosyltransferases / Q000096:biosynthesis*; D006863:Hydrogen-Ion Concentration; D007934:Leuconostoc / Q000201:enzymology*; D010710:Phosphates / Q000378:metabolism; D017680:Potassium Compounds / Q000378:metabolism; D013395:Sucrose / Q000378:metabolism; D013696:Temperature; D013997:Time Factors
chemical_list: D001426:Bacterial Proteins; D010710:Phosphates; D017680:Potassium Compounds; D013395:Sucrose; C013216:potassium phosphate; D005964:Glucosyltransferases; C020025:dextransucrase; D002122:Calcium Chloride