title: Selective Detection of Single-Stranded DNA Molecules Using a Glass Nanocapillary Functionalized with DNA.
abstract: We describe glass nanocapillaries with single-stranded DNA molecules (ssDNA) covalently attached to the capillary surface. These DNA-functionalized nanocapillaries selectively facilitate the translocation of target ssDNA that is complementary to the probe ssDNA. In addition, the complementary target ssDNA exhibits an event duration time longer than that of the noncomplementary target ssDNA. The temperature dependence measurements of translocation events show that the longer duration time is a result of an interaction between probe and target ssDNA and is dependent on the base pair binding strength. These results demonstrate that single-base mismatch transport selectivity can be achieved using the DNA-functionalized nanocapillaries.
authors.affiliation: Department of Physics, Yonsei University , Seoul 120-749, Republic of Korea.
authors.forename: Yeoan
authors.initials: Y
authors.lastname: Youn
authors.affiliation: Department of Physics, Yonsei University , Seoul 120-749, Republic of Korea.
authors.forename: Choongman
authors.initials: C
authors.lastname: Lee
authors.affiliation: Department of Physics, Yonsei University , Seoul 120-749, Republic of Korea.
authors.forename: Joo Hyoung
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Department of Materials Science and Engineering, Yonsei University , Seoul 120-749, Republic of Korea.
authors.forename: Young Wook
authors.initials: YW
authors.lastname: Chang
authors.affiliation: Department of Physics, Yonsei University , Seoul 120-749, Republic of Korea.
authors.forename: Dug Young
authors.initials: DY
authors.lastname: Kim
authors.affiliation: Department of Physics, Yonsei University , Seoul 120-749, Republic of Korea.
authors.forename: Kyung-Hwa
authors.initials: KH
authors.lastname: Yoo
mesh_terms: D004277:DNA, Single-Stranded / Q000032:analysis* / Q000737:chemistry*; D005898:Glass / Q000737:chemistry*; D006801:Humans; D043942:Nanotubes / Q000737:chemistry*; D013696:Temperature
chemical_list: D004277:DNA, Single-Stranded