title: Contribution of polyamines metabolism and GABA shunt to chilling tolerance induced by nitric oxide in cold-stored banana fruit.
abstract: Effect of exogenous nitric oxide (NO) on polyamines (PAs) catabolism, γ-aminobutyric acid (GABA) shunt, proline accumulation and chilling injury of banana fruit under cold storage was investigated. Banana fruit treated with NO sustained lower chilling injury index than the control. Notably elevated nitric oxide synthetase activity and endogenous NO level were observed in NO-treated banana fruit. PAs contents in treated fruit were significantly higher than control fruit, due to the elevated activities of arginine decarboxylase and ornithine decarboxylase. NO treatment increased the activities of diamine oxidase, polyamine oxidase and glutamate decarboxylase, while reduced GABA transaminase activity to lower levels compared with control fruit, which resulted the accumulation of GABA. Besides, NO treatment upregulated proline content and significantly enhanced the ornithine aminotransferase activity. These results indicated that the chilling tolerance induced by NO treatment might be ascribed to the enhanced catabolism of PAs, GABA and proline.
authors.affiliation: Zhejiang University, College of Biosystems Engineering and Food Science, Zhejiang Key Laboratory for Agro-Food Processing, Hangzhou 310058, People's Republic of China; Institute of Agro-Food Science and Technology, Shandong Academy of Agricultural Sciences, Jinan 250100, People's Republic of China.
authors.forename: Yansheng
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Zhejiang University, College of Biosystems Engineering and Food Science, Zhejiang Key Laboratory for Agro-Food Processing, Hangzhou 310058, People's Republic of China. Electronic address: luozisheng@zju.edu.cn.
authors.forename: Zisheng
authors.initials: Z
authors.lastname: Luo
authors.affiliation: Zhejiang University, College of Biosystems Engineering and Food Science, Zhejiang Key Laboratory for Agro-Food Processing, Hangzhou 310058, People's Republic of China.
authors.forename: Linchun
authors.initials: L
authors.lastname: Mao
authors.affiliation: Zhejiang University, College of Biosystems Engineering and Food Science, Zhejiang Key Laboratory for Agro-Food Processing, Hangzhou 310058, People's Republic of China.
authors.forename: Tiejin
authors.initials: T
authors.lastname: Ying
mesh_terms: D002262:Carboxy-Lyases / Q000378:metabolism; D003080:Cold Temperature; D061353:Food Storage; D005638:Fruit / Q000737:chemistry / Q000201:enzymology / Q000378:metabolism*; D005968:Glutamate Decarboxylase / Q000378:metabolism; D028521:Musa / Q000737:chemistry* / Q000201:enzymology / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism; D011073:Polyamines / Q000378:metabolism*; D011392:Proline / Q000378:metabolism; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
chemical_list: D010940:Plant Proteins; D011073:Polyamines; D009569:Nitric Oxide; D005680:gamma-Aminobutyric Acid; D011392:Proline; D002262:Carboxy-Lyases; D005968:Glutamate Decarboxylase; C019722:arginine decarboxylase
keywords: Banana fruit; Chilling injury; GABA; Nitric oxide; Polyamine; Proline