title: Methanol regulated yeast promoters: production vehicles and toolbox for synthetic biology.
abstract: Promoters are indispensable elements of a standardized parts collection for synthetic biology. Regulated promoters of a wide variety of well-defined induction ratios and expression strengths are highly interesting for many applications. Exemplarily, we discuss the application of published genome scale transcriptomics data for the primary selection of methanol inducible promoters of the yeast Pichia pastoris (Komagataella sp.). Such a promoter collection can serve as an excellent toolbox for cell and metabolic engineering, and for gene expression to produce heterologous proteins.
authors.affiliation: Department of Biotechnology, BOKU-University of Natural Resources and Life Sciences Vienna, Muthgasse 18, 1190, Vienna, Austria. brigitte.gasser@boku.ac.at.|Austrian Centre of Industrial Biotechnology (ACIB GmbH), Muthgasse 11, Vienna, Austria. brigitte.gasser@boku.ac.at.
authors.forename: Brigitte
authors.initials: B
authors.lastname: Gasser
authors.affiliation: Department of Biotechnology, BOKU-University of Natural Resources and Life Sciences Vienna, Muthgasse 18, 1190, Vienna, Austria. matthias.steiger@boku.ac.at.|Austrian Centre of Industrial Biotechnology (ACIB GmbH), Muthgasse 11, Vienna, Austria. matthias.steiger@boku.ac.at.
authors.forename: Matthias G
authors.initials: MG
authors.lastname: Steiger
authors.affiliation: Department of Biotechnology, BOKU-University of Natural Resources and Life Sciences Vienna, Muthgasse 18, 1190, Vienna, Austria. diethard.mattanovich@boku.ac.at.|Austrian Centre of Industrial Biotechnology (ACIB GmbH), Muthgasse 11, Vienna, Austria. diethard.mattanovich@boku.ac.at.
authors.forename: Diethard
authors.initials: D
authors.lastname: Mattanovich
mesh_terms: D015966:Gene Expression Regulation, Fungal; D060847:Metabolic Engineering; D000432:Methanol / Q000378:metabolism*; D010843:Pichia / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D058615:Synthetic Biology / Q000379:methods*
chemical_list: D011994:Recombinant Proteins; D000432:Methanol