title: Determination of xanthotoxin using a liquid chromatography-mass spectrometry and its application to pharmacokinetics and tissue distribution model in rat.
abstract: A simple and selective liquid chromatography mass spectrometry method for determination of xanthotoxin in rat plasma and various tissues for pharmacokinetic was developed. Chromatographic separation was achieved on a C18 (2.1 mm × 150 mm, 5 μm) column with acetonitrile-0.1% formic acid in water as mobile phase with gradient elution. An electrospray ionization source was applied and operated in positive ion mode; selective ion monitoring (SIM) mode was used for quantification using target fragment ions m/z 217 for xanthotoxin and m/z 326 for the internal standard. The resulting calibration curves offered satisfactory linearity (R(2) > 0.99) within the test range. Mean recoveries of xanthotoxin in rat plasma were in the range of 79.9%-84.6%. RSD of intra-day and inter-day precision were both < 14%. The accuracy of the method ranged from 87.5% to 109.8%. The assay was successfully applied to the pharmacokinetics and tissue distribution model studies of xanthotoxin in rats. The oral bioavailability of xanthotoxin was 73.2% in rats.
authors.affiliation: Department of Pharmacy, Lishui Central Hospital Lishui 323000, China.
authors.forename: Weiqiang
authors.initials: W
authors.lastname: Tian
authors.affiliation: The Second Affiliated & Yuying Children's Hospital Wenzhou Medical University Wenzhou 325000, China.
authors.forename: Jinzhang
authors.initials: J
authors.lastname: Cai
authors.affiliation: Department of Pharmacy, Lishui Central Hospital Lishui 323000, China.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Clinical Lab Medicine, Taizhou Municipal Hospital Affiliated with Taizhou University Taizhou 331000, China.
authors.forename: Xinhua
authors.initials: X
authors.lastname: Luo
authors.affiliation: Department of Clinical Lab Medicine, Taizhou Municipal Hospital Affiliated with Taizhou University Taizhou 331000, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Shanghai Institute of Pharmaceutical Industry Shanghai 200437, China.
authors.forename: Zixue
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Shanghai Institute of Pharmaceutical Industry Shanghai 200437, China.
authors.forename: Qingwei
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: The First Affiliated Hospital of Wenzhou Medical University Wenzhou 325035, China.
authors.forename: Xianqin
authors.initials: X
authors.lastname: Wang
authors.affiliation: The First Affiliated Hospital of Wenzhou Medical University Wenzhou 325035, China.
authors.forename: Lufeng
authors.initials: L
authors.lastname: Hu
authors.affiliation: The First Affiliated Hospital of Wenzhou Medical University Wenzhou 325035, China.
authors.forename: Guanyang
authors.initials: G
authors.lastname: Lin
keywords: Xanthotoxin; determination; pharmacokinetics; tissue distribution