title: MiR-145 suppresses cell proliferation and motility by inhibiting ROCK1 in hepatocellular carcinoma.
abstract: MicroRNAs (miRNAs) play key roles in cancer development and progression. In the present study, we investigated the role of miR-145 in the progression of hepatocellular carcinoma (HCC). Ten HCC cell lines and samples from 96 patients with HCC were analyzed for the expression of miR-145 by quantitative real-time polymerase chain reaction (qRT-PCR). Overexpression of miR-145 was established by transfecting mimics into HepG2 and QGY-7703 cells. Cell proliferation and cell migration were assessed by cell viability assay and transwell assay. Western blot was to verify ROCK1 as a novel target gene of miR-145. Our results showed that miR-145 was frequently downregulated in HCC tumors and cell lines. Overexpression of miR-145 in HCC cell lines significantly inhibited cell proliferation, migration, and invasion in vitro. ROCK1 was identified as a target of miR-145, and ectopic expression of miR-145 downregulated ROCK1. Together, these findings indicate that miR-145 acts as a tumor suppressor and its downregulation in tumor tissues may contribute to the progression and metastasis of HCC through a mechanism involving ROCK1, suggesting miR-145 as a potential new diagnostic and therapeutic target for the treatment of HCC.
authors.affiliation: Department of Clinical Laboratory, Kunming General Hospital, Chinese People's Liberation Army, Daguan Road, Kunming, 650032, Yunnan, China. drwanglan67@163.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Ding
authors.affiliation: Department of Orthopedics, Kunming General Hospital of Chinese People's Liberation Army, Daguan Road, Kunming, 650032, Yunnan, China.
authors.forename: Hongbo
authors.initials: H
authors.lastname: Tan
authors.affiliation: Department of Clinical Hematology, Third Military Medical University, Chongqing, 400038, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Zhao
authors.affiliation: Department of Clinical Laboratory, Kunming General Hospital, Chinese People's Liberation Army, Daguan Road, Kunming, 650032, Yunnan, China.
authors.forename: Xuemei
authors.initials: X
authors.lastname: Li
authors.affiliation: Division of Cellular and Molecular Diagnostics, Center of Laboratory Medicine, Chengdu Military General Hospital, Chengdu, 610083, Sichuan, China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Clinical Laboratory, Kunming General Hospital, Chinese People's Liberation Army, Daguan Road, Kunming, 650032, Yunnan, China.
authors.forename: Changli
authors.initials: C
authors.lastname: Jiang
authors.affiliation: Department of Clinical Laboratory, Kunming General Hospital, Chinese People's Liberation Army, Daguan Road, Kunming, 650032, Yunnan, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Clinical Laboratory, Kunming General Hospital, Chinese People's Liberation Army, Daguan Road, Kunming, 650032, Yunnan, China.
authors.forename: Lan
authors.initials: L
authors.lastname: Wang
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000473:pathology; D049109:Cell Proliferation / Q000235:genetics; D002470:Cell Survival / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D054460:rho-Associated Kinases / Q000096:biosynthesis* / Q000235:genetics
chemical_list: C541010:MIRN145 microRNA, human; D035683:MicroRNAs; C515851:ROCK1 protein, human; D054460:rho-Associated Kinases
keywords: Hepatocellular carcinoma; MiR-145; Migration; Proliferation; ROCK1