title: Protective role of methylprednisolone and heparin in ischaemic-reperfusion injury of the rat testicle.
abstract: This study evaluated the therapeutic efficacy of heparin and methylprednisolone in the treatment of ischaemic reperfusion (IR) injury of the testis. Twenty-four male Sprague-Dawley rats were allocated equally into three groups of eight animals each. The left testes were rotated 720° for 2 h in the rats in the torsion-detorsion group. Rats in the treatment groups underwent the same surgical procedure as the torsion-detorsion group but were also given methylprednisolone (group II) or heparin (group III) by an intraperitoneal route 30 min prior to detorsion. Left orchiectomy was performed in all rats from each experimental animal at 2 h after detorsion, and the tissue was harvested for the measurement of malondialdehyde (MDA), protein carbonyl (PC) and nitric oxide (NO) and the endogenous antioxidant enzymes, superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase. Additional tissue was evaluated using histopathological and immunohistochemical changes. PC and MDA levels were significantly reduced in the treated groups compared to the control group. There was no statistically significant difference in NO level or SOD, GSH-Px and catalase activity among the treatment groups. Histopathological and immunohistochemical findings supported biochemical changes. It is concluded that pre-treatment with methylprednisolone or heparin protects the testis in ischaemic reperfusion injury caused by testicular torsion-detorsion.
authors.affiliation: Medical Biochemistry, Faculty of Medicine, Erzincan University, Erzincan, Turkey.
authors.forename: C
authors.initials: C
authors.lastname: Mertoğlu
authors.affiliation: Pediatric Surgery, Faculty of Medicine, Ufuk Şenel: Gaziosmanpasa University, Tokat, Turkey.
authors.forename: U
authors.initials: U
authors.lastname: Senel
authors.affiliation: Histology and Embryology, Faculty of Medicine, Yildirim Beyazit University, Ankara, Turkey.
authors.forename: S
authors.initials: S
authors.lastname: Cayli
authors.affiliation: Anatomy, Faculty of Medicine, Gaziosmanpasa University, Tokat, Turkey.
authors.forename: U
authors.initials: U
authors.lastname: Tas
authors.affiliation: Biochemistry, Bingol State Hospital, Bingol, Turkey.
authors.forename: Z
authors.initials: Z
authors.lastname: Küskü Kiraz
authors.affiliation: Medical Biochemistry, Faculty of Medicine, Gaziosmanpasa University, Tokat, Turkey.
authors.forename: H
authors.initials: H
authors.lastname: Özyurt
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use*; D000925:Anticoagulants / Q000627:therapeutic use*; D002374:Catalase / Q000378:metabolism; D004195:Disease Models, Animal; D005979:Glutathione Peroxidase / Q000378:metabolism; D006493:Heparin / Q000627:therapeutic use*; D007150:Immunohistochemistry; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008775:Methylprednisolone / Q000627:therapeutic use*; D009569:Nitric Oxide / Q000378:metabolism; D009919:Orchiectomy; D050050:Protein Carbonylation / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000378:metabolism; D013086:Spermatic Cord Torsion / Q000150:complications*; D013482:Superoxide Dismutase / Q000378:metabolism; D013737:Testis / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D016896:Treatment Outcome
chemical_list: D000893:Anti-Inflammatory Agents; D000925:Anticoagulants; D009569:Nitric Oxide; D008315:Malondialdehyde; D006493:Heparin; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D008775:Methylprednisolone
keywords: Heparin; ischaemic reperfusion; methylprednisolone; testicle