title: Incorporation of copper ions into crystals of T2 copper-depleted laccase from Botrytis aclada.
abstract: Laccases belong to the class of multicopper oxidases catalyzing the oxidation of phenols accompanied by the reduction of molecular oxygen to water without the formation of hydrogen peroxide. The activity of laccases depends on the number of Cu atoms per enzyme molecule. The structure of type 2 copper-depleted laccase from Botrytis aclada has been solved previously. With the aim of obtaining the structure of the native form of the enzyme, crystals of the depleted laccase were soaked in Cu(+)- and Cu(2+)-containing solutions. Copper ions were found to be incorporated into the active site only when Cu(+) was used. A comparative analysis of the native and depleted forms of the enzymes was performed.
authors.affiliation: Laboratory of Enzyme Engineering, A. N. Bach Institute of Biochemistry, Leninsky pr. 33, Moscow 119071, Russian Federation.
authors.forename: E M
authors.initials: EM
authors.lastname: Osipov
authors.affiliation: Laboratory of Enzyme Engineering, A. N. Bach Institute of Biochemistry, Leninsky pr. 33, Moscow 119071, Russian Federation.
authors.forename: K M
authors.initials: KM
authors.lastname: Polyakov
authors.affiliation: Laboratory of Enzyme Engineering, A. N. Bach Institute of Biochemistry, Leninsky pr. 33, Moscow 119071, Russian Federation.
authors.forename: T V
authors.initials: TV
authors.lastname: Tikhonova
authors.affiliation: Department of Food Science and Technology, BOKU - University of Natural Resources and Life Sciences, Muthgasse 18, 1190 Wien, Austria.
authors.forename: R
authors.initials: R
authors.lastname: Kittl
authors.affiliation: RSC `Kurchatov Institute', Acad. Kurchatov sq. 1, Moscow 123182, Russian Federation.
authors.forename: P V
authors.initials: PV
authors.lastname: Dorovatovskii
authors.affiliation: Laboratory of Enzyme Engineering, A. N. Bach Institute of Biochemistry, Leninsky pr. 33, Moscow 119071, Russian Federation.
authors.forename: S V
authors.initials: SV
authors.lastname: Shleev
authors.affiliation: Laboratory of Enzyme Engineering, A. N. Bach Institute of Biochemistry, Leninsky pr. 33, Moscow 119071, Russian Federation.
authors.forename: V O
authors.initials: VO
authors.lastname: Popov
authors.affiliation: Department of Food Science and Technology, BOKU - University of Natural Resources and Life Sciences, Muthgasse 18, 1190 Wien, Austria.
authors.forename: R
authors.initials: R
authors.lastname: Ludwig
mesh_terms: D000595:Amino Acid Sequence; D020171:Botrytis / Q000201:enzymology*; D003300:Copper / Q000378:metabolism*; D003460:Crystallization; D018360:Crystallography, X-Ray; D007477:Ions; D042845:Laccase / Q000737:chemistry / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D013696:Temperature
chemical_list: D007477:Ions; D003300:Copper; D042845:Laccase
keywords: copper(I) complex; full copper content; laccase