title: Periodic Nanoneedle and Buffer Zones Constructed on a Titanium Surface Promote Osteogenic Differentiation and Bone Calcification In Vivo.
abstract: Rapid and effective bone mineralization at the bone/implant interface is required for successful orthopedic and dental implants. In this study, two periodic microscale functionalized zones on titanium (MZT) are created, namely, nanoneedle zones and buffer zones. The aim of this design is to provide spatially regulated topographical cues on titanium to enhance the efficacy of bone regeneration. This goal is achieved using a versatile and effective technique in which nanoneedle structures are hydrothermally constructed on the surface of titanium sheets, after which selective laser irradiation is used to construct buffer zones. The zonal structures of the MZT overcome the suppressive effect of the nanoneedle film on osteoblasts. Additionally, the MZT exhibits zone-selective apatite deposition and protein adsorption. The accelerated in vitro osteoblast differentiation and nodule deposition on the MZT are confirmed. Elemental analysis of the bone nodules formed by the osteoblasts growing on the titanium and MZT demonstrates they have different compositions. Histological and scanning electron microscope analysis of the bone formation on in vivo implants shows that this process is also enhanced by the MZT implant. The concept of constructing functionalized zones on titanium implant could facilitate future research on improving the design of orthopedic and dental implant surfaces.
authors.affiliation: School of Materials Science and Engineering, South China University of Technology, Guangzhou, 510641, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Yu
authors.affiliation: Department of Dentistry, Baoan Central Hospital of Shenzhen, Shenzhen, 518102, China.
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Zhu
authors.affiliation: School of Materials Science and Engineering, South China University of Technology, Guangzhou, 510641, China.
authors.forename: Xiaolan
authors.initials: X
authors.lastname: Wang
authors.affiliation: School of Materials Science and Engineering, South China University of Technology, Guangzhou, 510641, China.
authors.forename: Shuangying
authors.initials: S
authors.lastname: Wang
authors.affiliation: School of Materials Science and Engineering, South China University of Technology, Guangzhou, 510641, China.
authors.forename: Weiping
authors.initials: W
authors.lastname: Li
authors.affiliation: Institute of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou, 510006, China.
authors.forename: Guoxin
authors.initials: G
authors.lastname: Tan
authors.affiliation: General Hospital of Guangzhou Military Command of PLA, Guangzhou, 510010, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: School of Materials Science and Engineering, South China University of Technology, Guangzhou, 510641, China.
authors.forename: Chengyun
authors.initials: C
authors.lastname: Ning
mesh_terms: D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects; D001842:Bone and Bones / Q000187:drug effects / Q000502:physiology; D002113:Calcification, Physiologic / Q000187:drug effects*; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D015921:Dental Implants; D051379:Mice; D049329:Nanostructures / Q000008:administration & dosage*; D036103:Nanotechnology / Q000379:methods; D009339:Needles; D010006:Osteoblasts / Q000187:drug effects; D010012:Osteogenesis / Q000187:drug effects*; D010550:Personal Space; D013499:Surface Properties / Q000187:drug effects*; D014025:Titanium / Q000008:administration & dosage*
chemical_list: D015921:Dental Implants; D014025:Titanium
keywords: bone minerals; buffer zones; nanoneedle films; osteogenic differentiation, titanium