title: Silole-Containing Polymer Nanodot: An Aqueous Low-Potential Electrochemiluminescence Emitter for Biosensing.
abstract: A novel D-A conjugated polymer backbone containing silole and 9-octyl-9H-carbazole units was synthesized via Sonogashira reaction. This silole-containing polymer (SCP) was further used to prepare SCP dots with a nanoprecipitation method, which showed an electrochemiluminescence (ECL) emission at relatively low potential in aqueous solution. The strong anodic ECL emission could be observed at +0.4 V (vs Ag/AgCl) with a peak value at +0.78 V in the presence of tri-n-propylamine (TPrA) as a co-reactant, which came from the band gap emission of the excited SCP dots. The ECL emission could be quenched via resonance energy transfer from the excited SCP dots to an acceptor. Thus, a low-potential anodic ECL sensing strategy was proposed for ECL detection of the acceptor-related analytes. Using dopamine as the analyte, whose electro-oxidation product could act as the energy acceptor to quench the ECL emission of SCP dots, the ECL detection method showed a detection limit of 50 nM and high anti-interference ability. This work demonstrates an example of polymer dots as an ECL emitter and its potential application in ECL detection methodology.
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University , Nanjing 210023, P. R. China.
authors.forename: Yaqiang
authors.initials: Y
authors.lastname: Feng
authors.affiliation: Key Lab of Mesoscopic Chemistry of MOE, School of Chemistry and Chemical Engineering, Nanjing University , Nanjing 210023, P. R. China.
authors.forename: Chunhui
authors.initials: C
authors.lastname: Dai
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University , Nanjing 210023, P. R. China.
authors.forename: Jianping
authors.initials: J
authors.lastname: Lei
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University , Nanjing 210023, P. R. China.
authors.forename: Huangxian
authors.initials: H
authors.lastname: Ju
authors.affiliation: Key Lab of Mesoscopic Chemistry of MOE, School of Chemistry and Chemical Engineering, Nanjing University , Nanjing 210023, P. R. China.
authors.forename: Yixiang
authors.initials: Y
authors.lastname: Cheng
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D004298:Dopamine / Q000032:analysis*; D055664:Electrochemical Techniques; D049449:Luminescence; D015394:Molecular Structure; D053758:Nanoparticles / Q000737:chemistry*; D010084:Oxidation-Reduction; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D012821:Silanes / Q000138:chemical synthesis / Q000737:chemistry*; D014867:Water / Q000737:chemistry
chemical_list: D011108:Polymers; D012821:Silanes; C540376:silole; D014867:Water; D004298:Dopamine