title: RUNX3 gene polymorphisms and haplotypes in Mexican patients with colorectal cancer.
abstract: We analyzed a possible association between RUNX3 gene polymorphisms and haplotypes in Mexican patients with colorectal cancer (CRC). Genomic DNA samples were obtained from the peripheral blood of 176 Mexican patients with CRC at diagnosis and from 195 individuals that formed the control group. The polymorphisms were detected by polymerase chain reaction-restriction fragment length polymorphism. Association was estimated by odds ratio (OR). The haplotypes and linkage disequilibrium were established using the Arlequin v3.5 software. We found that the RUNX3 polymorphisms analyzed were in Hardy-Weinberg equilibrium. The RUNX3 rs2236852 AA genotype and A allele showed association with CRC (OR = 0.39, 95%CI = 0.21-0.73, P < 0.01; OR = 0.65, 95%CI = 0.49-0.87, P < 0.01, respectively), while the rs6672420, rs11249206, and rs760805 polymorphisms did not show significant association with CRC. The TA haplotype (SNPs rs760805 and rs2236852) showed an increased risk for CRC (OR = 2.52, 95%CI = 1.47-4.30, P < 0.001). In conclusion, we found that the AA genotype and A allele of rs2236852 polymorphism confer a decreased CRC risk, while the TA haplotype appears to increase the risk of CRC development in Mexican patients.
authors.affiliation: Doctorado en Genética Humana and Instituto de Genética Humana, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Jalisco, México.
authors.forename: S
authors.initials: S
authors.lastname: Suárez-Villanueva
authors.affiliation: Doctorado en Genética Humana and Instituto de Genética Humana, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Jalisco, México.
authors.forename: M L
authors.initials: ML
authors.lastname: Ayala-Madrigal
authors.affiliation: Laboratorio de Inmunobiología, Centro Universitario de Ciencias Biológicas y Agropecuarias, Universidad de Guadalajara, Zapopan, Jalisco, México.
authors.forename: J
authors.initials: J
authors.lastname: Peregrina-Sandoval
authors.affiliation: Laboratorio de Genética Humana, Centro Universitario del Sur, Universidad de Guadalajara, Ciudad Guzmán, Jalisco, México.
authors.forename: N
authors.initials: N
authors.lastname: Macías-Gómez
authors.affiliation: Doctorado en Genética Humana and Instituto de Genética Humana, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Jalisco, México.
authors.forename: R
authors.initials: R
authors.lastname: Ramírez-Ramírez
authors.affiliation: Doctorado en Genética Humana and Instituto de Genética Humana, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Jalisco, México.
authors.forename: R
authors.initials: R
authors.lastname: Muñiz-Mendoza
authors.affiliation: Doctorado en Genética Humana and Instituto de Genética Humana, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Jalisco, México.
authors.forename: J M
authors.initials: JM
authors.lastname: Moreno-Ortiz
authors.affiliation: Hospital Civil de Guadalajara "Dr. Juan I. Menchaca", Guadalajara, Jalisco, México.
authors.forename: M
authors.initials: M
authors.lastname: Centeno-Flores
authors.affiliation: Hospital Civil de Guadalajara "Dr. Juan I. Menchaca", Guadalajara, Jalisco, México.
authors.forename: V
authors.initials: V
authors.lastname: Maciel-Gutiérrez
authors.affiliation: Hospital Civil de Guadalajara “Fray Antonio Alcalde”, Guadalajara, Jalisco, México
authors.forename: E
authors.initials: E
authors.lastname: Cabrales
authors.affiliation: Doctorado en Genética Humana and Instituto de Genética Humana, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Jalisco, México.
authors.forename: M
authors.initials: M
authors.lastname: Gutiérrez-Angulo
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D016022:Case-Control Studies; D015179:Colorectal Neoplasms / Q000235:genetics*; D050677:Core Binding Factor Alpha 3 Subunit / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D006239:Haplotypes; D006801:Humans; D008297:Male; D008800:Mexico; D008875:Middle Aged; D016017:Odds Ratio; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors; D055815:Young Adult
chemical_list: D050677:Core Binding Factor Alpha 3 Subunit