title: Inducing Pluripotency in Cattle.
abstract: Nuclear reprogramming technologies in general and induced pluripotent stem cells (iPSCs) in particular have opened the door to a vast number of practical applications in regenerative medicine and biotechnology. It also represents a possible alternative to the still evasive achievement of embryonic stem cells (ESCs) isolation from refractory species such as Bos. taurus. Herein, we described a protocol for bovine iPSCs (biPSCs) generation and characterization. The protocol is based on the overexpression of the exogenous transcription factors NANOG, OCT4, SOX2, KLF4 and c-MYC, using a pantropic retroviral system.
authors.affiliation: Monash Institute for Medical Research, Monash University, Clayton, VIC, 3168, Australia. luismalaver@gmail.com.|Australian Animal Health Laboratories, CSIRO Biosecurity Flagship, East Geelong, VIC, 3219, Australia. luismalaver@gmail.com.
authors.forename: Luis F
authors.initials: LF
authors.lastname: Malaver-Ortega
authors.affiliation: Department of Animal Science, Ferdowsi University of Mashhad, Mashhad, Iran.
authors.forename: Amir
authors.initials: A
authors.lastname: Taheri-Ghahfarokhi
authors.affiliation: Department of Chemistry and Biotechnology, Faculty of Science, Engineering and Technology, Swinburne University of Technology, Hawthorn, VIC, 3122, Australia.
authors.forename: Huseyin
authors.initials: H
authors.lastname: Sumer
mesh_terms: D000818:Animals; D002417:Cattle; D018929:Cell Culture Techniques; D002471:Cell Transformation, Neoplastic; D065150:Cellular Reprogramming; D004195:Disease Models, Animal; D058732:Embryoid Bodies; D005260:Female; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism*; D005822:Genetic Vectors / Q000235:genetics; D064593:Heterografts; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism*; D000090062:Kruppel-Like Factor 4; D051379:Mice; D012190:Retroviridae / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013724:Teratoma / Q000235:genetics / Q000473:pathology; D014161:Transduction, Genetic; D019076:Transgenes
keywords: Bos. taurus; Bovine; Nuclear reprogramming; Pluripotency; Retrovirus; Stem cells; Transduction; iPSCs