title: IARS2 silencing induces non-small cell lung cancer cells proliferation inhibition, cell cycle arrest and promotes cell apoptosis.
abstract: The purpose of this study was to investigate the potential role of Ileucyl-tRNA synthetase (IARS2) silencing in non-small cell lung cancer (NSCLC). The silencing of IARS2 in H1299 cells and A549 cells were performed by lentivirus encoding shRNAs. The efficiency of IARS2 silencing was detected by quantitative real time PCR and western blot. The effects of IARS2 silencing on cell growth, cell apoptosis, cell cycle and cell colony formation ability were assessed by cells counting, MTT assay, flow cytometer analysis and soft agar colony formation assay, respectively. Compared with negative control group, IARS2 was significantly knockdown by transfection with lentivirus encoding shRNA of IARS2. The IARS2 silencing significantly inhibited the cells proliferation and cells colony formation ability, induced cell cycle arrest at G1/S phase and promoted cell apoptosis. IARS2 silencing induced NSCLC cells growth inhibition, cell cycle arrest and promoted cell apoptosis. These results suggest that IARS2 may be a novel target for the treatment of NSCLC.
authors.forename: J
authors.initials: J
authors.lastname: Yin
authors.forename: W
authors.initials: W
authors.lastname: Liu
authors.forename: R
authors.initials: R
authors.lastname: Li
authors.forename: J
authors.initials: J
authors.lastname: Liu
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
authors.forename: W
authors.initials: W
authors.lastname: Tang
authors.forename: K
authors.initials: K
authors.lastname: Wang
mesh_terms: D000604:Amino Acyl-tRNA Synthetases / Q000235:genetics* / Q000378:metabolism; D017209:Apoptosis; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics; D002453:Cell Cycle; D059447:Cell Cycle Checkpoints / Q000235:genetics / Q000502:physiology; D049109:Cell Proliferation / Q000187:drug effects / Q000502:physiology; D020868:Gene Silencing; D006801:Humans; D016086:Lentivirus / Q000235:genetics; D008175:Lung Neoplasms / Q000235:genetics; D014162:Transfection
chemical_list: D000604:Amino Acyl-tRNA Synthetases; C549096:LARS2 protein, human
keywords: IARS2 silencing; NSCLC; cell apoptosis; cell cycle.; cell proliferation