title: Autoantibody Profile of a Cohort of 78 Italian Patients with Mucous Membrane Pemphigoid: Correlation Between Reactivity Profile and Clinical Involvement.
abstract: Direct diagnosis of mucous membrane pemphigoid (MMP) is not easy. Circulating autoantibodies targeting bullous pemphigoid antigens of 180 kDa and 230 kDa (BP180 and BP230), α6β4 integrin, laminin 332 and type VII collagen (Col VII) are not always present. The aims of this study were to characterize the humoral immune response of a cohort of Italian patients with MMP, its association with clinical involvement and severity, and to design an algorithm for efficient serological diagnosis. Seventy-eight MMP sera were studied retrospectively by indirect immunofluorescence on salt-split skin, enzyme-linked immunosorbent assay (ELISA) and immunoblotting. Indirect immunofluorescence on salt-split skin resulted in the most sensitive approach for diagnosis of MMP. BP180 was the major autoantigen in MMP patients with oral and cutaneous involvement. Significant associations were found between BP180 reactivity and oral and cutaneous localization of the lesions (p = 0.006), and between Col VII positivity and Setterfield severity score (p = 0.020).
authors.affiliation: Department of Dermatology, IRCCS AOU san martino-IST, DISSAL, Sezione di Dermatologia , University of Genoa, IT-16100 Genova, Italy. emanuele.cozzani@unige.it.
authors.forename: Emanuele
authors.initials: E
authors.lastname: Cozzani
authors.forename: Giovanni
authors.initials: G
authors.lastname: Di Zenzo
authors.forename: Valentina
authors.initials: V
authors.lastname: Calabresi
authors.forename: Marco
authors.initials: M
authors.lastname: Carrozzo
authors.forename: Martina
authors.initials: M
authors.lastname: Burlando
authors.forename: Lora
authors.initials: L
authors.lastname: Longanesi
authors.forename: Amilcare
authors.initials: A
authors.lastname: Cerri
authors.forename: Marzia
authors.initials: M
authors.lastname: Caproni
authors.forename: Francesco
authors.initials: F
authors.lastname: Sera
authors.forename: Emiliano
authors.initials: E
authors.lastname: Antiga
authors.forename: Pietro
authors.initials: P
authors.lastname: Quaglino
authors.forename: Angelo V
authors.initials: AV
authors.lastname: Marzano
authors.forename: Aurora
authors.initials: A
authors.lastname: Parodi
mesh_terms: D000368:Aged; D001323:Autoantibodies / Q000097:blood*; D024143:Collagen Type VII / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D056724:Immunity, Humoral; D015151:Immunoblotting; D007558:Italy; D008297:Male; D024041:Non-Fibrillar Collagens / Q000276:immunology; D010390:Pemphigoid, Benign Mucous Membrane / Q000276:immunology*; D012189:Retrospective Studies; D012720:Severity of Illness Index
chemical_list: D001323:Autoantibodies; D024143:Collagen Type VII; D024041:Non-Fibrillar Collagens