title: Power-Frequency Magnetic Field Inhibits Adipogenic Differentiation in Human ADSC.
abstract: OBJECTIVE
  Semicircular lipoatrophy (SL) is an idiopathic condition characterized by atrophy of subcutaneous fatty tissue. Although several studies have suggested a possible association between SL and occupational exposure to power frequency magnetic fields (MF), no mechanism has been proposed so far that explains an influence of these fields on adipogenesis.
  
  
  METHODS
  The study investigates the effects of a 50 Hz, 100 µT MF on the adipogenesis of stem cells isolated from human adipose tissue (ADSC). Cells were plated in Petri dishes and either exposed intermittently to the field for 42 hours or sham-exposed.
  
  
  RESULTS
  Field exposure significantly reduced lipid accumulation within the cells, revealed in Oil Red O stained samples by spectrophotometry and colorimetry. Early cell passages were particularly sensitive to the effect: 30.40 ± 5.77% and 47.96 ± 12.47% below controls in the spectrophotometric and colorimetric assays, respectively. Such antiadipogenic effect was accompanied by significant changes in the expression of key effectors/regulators of early adipogenesis: PPARx03B3;, p-ERK1/2 and Sox9, indicating that at least the ERK/PPARx03B3; signaling pathway could be involved in the effect.
  
  
  CONCLUSIONS
  These results constitute an experimental support to the hypothesis that power frequency MF can be one of the factors involved in the etiology of SL.
authors.forename: María Antonia
authors.initials: MA
authors.lastname: Martínez
authors.forename: María Ángeles
authors.initials: MÁ
authors.lastname: Trillo
authors.forename: María Antonia
authors.initials: MA
authors.lastname: Cid
authors.forename: Carlos L
authors.initials: CL
authors.lastname: Paíno
authors.forename: Alejandro
authors.initials: A
authors.lastname: Úbeda
mesh_terms: D000273:Adipose Tissue / Q000166:cytology* / Q000378:metabolism; D002454:Cell Differentiation; D002478:Cells, Cultured; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D050356:Lipid Metabolism; D020935:MAP Kinase Signaling System; D008280:Magnetics; D047495:PPAR gamma / Q000378:metabolism; D055758:SOX9 Transcription Factor / Q000378:metabolism
chemical_list: D047495:PPAR gamma; D055758:SOX9 Transcription Factor; C527932:SOX9 protein, human