title: Viral Vector Production: Adenovirus.
abstract: Adenoviral vectors have proven to be valuable resources in the development of novel therapies aimed at targeting pathological conditions of the central nervous system, including Alzheimer's disease and neoplastic brain lesions. Not only can some genetically engineered adenoviral vectors achieve remarkably efficient and specific gene delivery to target cells, but they also may act as anticancer agents by selectively replicating within cancer cells.Due to the great interest in using adenoviral vectors for various purposes, the need for a comprehensive protocol for viral vector production is especially apparent. Here, we describe the process of generating an adenoviral vector in its entirety, including the more complex process of adenoviral fiber modification to restrict viral tropism in order to achieve more efficient and specific gene delivery.
authors.affiliation: The University of Chicago Medicine, 5841 S. Maryland Avenue, MC 3026, Chicago, IL, USA.
authors.forename: Julius W
authors.initials: JW
authors.lastname: Kim
authors.affiliation: The University of Chicago Medicine, 5841 S. Maryland Avenue, MC 3026, Chicago, IL, USA.
authors.forename: Ramin A
authors.initials: RA
authors.lastname: Morshed
authors.affiliation: The University of Chicago Medicine, 5841 S. Maryland Avenue, MC 3026, Chicago, IL, USA.
authors.forename: J Robert
authors.initials: JR
authors.lastname: Kane
authors.affiliation: The University of Chicago Medicine, 5841 S. Maryland Avenue, MC 3026, Chicago, IL, USA.
authors.forename: Brenda
authors.initials: B
authors.lastname: Auffinger
authors.affiliation: The University of Chicago Medicine, 5841 S. Maryland Avenue, MC 3026, Chicago, IL, USA.
authors.forename: Jian
authors.initials: J
authors.lastname: Qiao
authors.affiliation: The University of Chicago Medicine, 5841 S. Maryland Avenue, MC 3026, Chicago, IL, USA. mlesniak@surgery.bsd.uchicago.edu.
authors.forename: Maciej S
authors.initials: MS
authors.lastname: Lesniak
mesh_terms: D000256:Adenoviridae / Q000235:genetics / Q000254:growth & development* / Q000502:physiology; D005822:Genetic Vectors / Q000096:biosynthesis; D057809:HEK293 Cells; D006801:Humans; D056189:Viral Tropism
keywords: Adenoviral gene therapy; Adenovirus; Adenovirus fiber modification; Adenovirus tropism; Viral vector production