title: Development of germ cell neoplasia in situ in chinchilla rabbits.
abstract: The present study was designed to describe the development of germ cell neoplasia in situ in Chinchilla rabbit by administration of estradiol. The study was performed in rabbits distributed into two groups: control and 17 β-estradiol. The determination of histological alterations and POU5F1 and c-kit proteins employed as biomarkers for the diagnosis of this neoplasia was carried out. Testicular descent and complete spermatogenesis were observed in the control group. The protein biomarkers were negative. However, in the rabbits treated with estradiol, the testes remained undescended with the gonocytes undifferentiated to spermatogonia. There were histological lesions owing to germ cell neoplasia in situ and positive to POU5F1 and c-kit proteins. These findings indicate that the chinchilla rabbit is an ideal model to study this neoplasia in which the histological characteristics and biomarkers of the disease could be clearly observed. Using this model we suggested that the persisting gonocytes could be responsible for the development of germ cell neoplasia in situ.
authors.affiliation: Laboratory of Reproductive Biology, National Institute of Pediatrics and Faculty of Veterinary Medicine and Animal Husbandry, National Autonomous University of Mexico, Mexico City, Mexico. rmvigueras@yahoo.com.mx.
authors.forename: Rosa María
authors.initials: RM
authors.lastname: Vigueras-Villaseñor
authors.affiliation: Laboratory of Reproductive Biology, National Institute of Pediatrics, Mexico City, Mexico.
authors.forename: Paola
authors.initials: P
authors.lastname: Montelongo Solís
authors.affiliation: Laboratory of Reproductive Biology, National Institute of Pediatrics, Mexico City, Mexico.
authors.forename: Margarita
authors.initials: M
authors.lastname: Chávez-Saldaña
authors.affiliation: Faculty of Veterinary Medicine and Animal Husbandry, National Autonomous University of Mexico, Mexico City, Mexico.
authors.forename: Oscar
authors.initials: O
authors.lastname: Gutiérrez-Pérez
authors.affiliation: Laboratory of Reproductive Biology, National Institute of Pediatrics, Mexico City, Mexico.
authors.forename: Lucero
authors.initials: L
authors.lastname: Cortés Trujillo
authors.affiliation: Laboratory of Reproductive Biology, National Institute of Pediatrics, Mexico City, Mexico. rocajc1@yahoo.com.mx.
authors.forename: Julio César
authors.initials: JC
authors.lastname: Rojas-Castañeda
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000032:analysis; D002278:Carcinoma in Situ / Q000473:pathology*; D002682:Chinchilla; D004195:Disease Models, Animal; D007150:Immunohistochemistry; D008297:Male; D046529:Microscopy, Electron, Transmission; D050814:Octamer Transcription Factor-3 / Q000032:analysis; D019009:Proto-Oncogene Proteins c-kit / Q000032:analysis; D011817:Rabbits; D060888:Real-Time Polymerase Chain Reaction; D013736:Testicular Neoplasms / Q000473:pathology*
chemical_list: D014408:Biomarkers, Tumor; D050814:Octamer Transcription Factor-3; D019009:Proto-Oncogene Proteins c-kit