title: Loss of Periostin Results in Impaired Regeneration and Pancreatic Atrophy after Cerulein-Induced Pancreatitis.
abstract: The extracellular matrix molecule periostin (POSTN, encoded by POSTN), which is secreted by activated pancreatic stellate cells, has important functions in chronic pancreatitis and pancreatic cancer. However, the role of POSTN in acute pancreatitis and subsequent regeneration processes has not been addressed so far. We analyzed the function of POSTN in pancreatic exocrine regeneration after the induction of a severe acute pancreatitis. Postn-deficient mice and wild-type control animals received repetitive cerulein injections, and a detailed histologic analysis of pancreatic tissues was performed. Although there was no difference in pancreatitis severity in the acute inflammatory phase, the recovery of the exocrine pancreas was massively impaired in Postn-deficient mice. Loss of Postn expression was accompanied by strong pancreatic atrophy and acinar-to-adipocyte differentiation, which was also reflected in gene expression patterns. Our data suggest that POSTN is a crucial factor for proper exocrine lineage-specific regeneration after severe acute pancreatitis.
authors.affiliation: Department of Surgery, Technische Universität München, Munich, Germany.
authors.forename: Simone
authors.initials: S
authors.lastname: Hausmann
authors.affiliation: Department of Surgery, Technische Universität München, Munich, Germany; Institute of Pathology, Heinrich-Heine University, Düsseldorf, Germany.
authors.forename: Ivonne
authors.initials: I
authors.lastname: Regel
authors.affiliation: Institute of Pathology, Technische Universität München, Munich, Germany.
authors.forename: Katja
authors.initials: K
authors.lastname: Steiger
authors.affiliation: Department of Surgery, Technische Universität München, Munich, Germany; Institute of Pathology, Heinrich-Heine University, Düsseldorf, Germany.
authors.forename: Nadine
authors.initials: N
authors.lastname: Wagner
authors.affiliation: Department of Surgery, Technische Universität München, Munich, Germany.
authors.forename: Manja
authors.initials: M
authors.lastname: Thorwirth
authors.affiliation: Institute of Pathology, Technische Universität München, Munich, Germany.
authors.forename: Anna M
authors.initials: AM
authors.lastname: Schlitter
authors.affiliation: Institute of Pathology, Heinrich-Heine University, Düsseldorf, Germany; Institute of Pathology, Technische Universität München, Munich, Germany.
authors.forename: Irene
authors.initials: I
authors.lastname: Esposito
authors.affiliation: Department of Surgery, Technische Universität München, Munich, Germany; Department of Surgery, University of Heidelberg, Heidelberg, Germany.
authors.forename: Christoph W
authors.initials: CW
authors.lastname: Michalski
authors.affiliation: Department of Surgery, Technische Universität München, Munich, Germany.
authors.forename: Helmut
authors.initials: H
authors.lastname: Friess
authors.affiliation: Department of Surgery, Technische Universität München, Munich, Germany; Royal Liverpool and Broadgreen University Hospitals, Liverpool, United Kingdom; Department of Surgery, Heinrich-Heine University, Düsseldorf, Germany.
authors.forename: Jörg
authors.initials: J
authors.lastname: Kleeff
authors.affiliation: Department of Surgery, Technische Universität München, Munich, Germany; Department of Surgery, Koc School of Medicine, Istanbul, Turkey. Electronic address: merkan@ku.edu.tr.
authors.forename: Mert
authors.initials: M
authors.lastname: Erkan
mesh_terms: D000818:Animals; D001284:Atrophy; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D002108:Ceruletide / Q000633:toxicity; D004195:Disease Models, Animal; D007150:Immunohistochemistry; D051379:Mice; D018345:Mice, Knockout; D046790:Pancreas, Exocrine / Q000473:pathology / Q000502:physiology; D010195:Pancreatitis / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology*; D060888:Real-Time Polymerase Chain Reaction; D012038:Regeneration
chemical_list: D015815:Cell Adhesion Molecules; C082838:Postn protein, mouse; D002108:Ceruletide