title: Minor Role of Plasminogen in Complement Activation on Cell Surfaces.
abstract: Atypical hemolytic uremic syndrome (aHUS) is a rare, but severe thrombotic microangiopathy. In roughly two thirds of the patients, mutations in complement genes lead to uncontrolled activation of the complement system against self cells. Recently, aHUS patients were described with deficiency of the fibrinolytic protein plasminogen. This zymogen and its protease form plasmin have both been shown to interact with complement proteins in the fluid phase. In this work we studied the potential of plasminogen to restrict complement propagation. In hemolytic assays, plasminogen inhibited complement activation, but only when it had been exogenously activated to plasmin and when it was used at disproportionately high concentrations compared to serum. Addition of only the zymogen plasminogen into serum did not hinder complement-mediated lysis of erythrocytes. Plasminogen could not restrict deposition of complement activation products on endothelial cells either, as was shown with flow cytometry. With platelets, a very weak inhibitory effect on deposition of C3 fragments was observed, but it was considered too weak to be significant for disease pathogenesis. Thus it was concluded that plasminogen is not an important regulator of complement on self cells. Instead, addition of plasminogen was shown to clearly hinder platelet aggregation in serum. This was attributed to plasmin causing disintegration of formed platelet aggregates. We propose that reduced proteolytic activity of plasmin on structures of growing thrombi, rather than on complement activation fragments, explains the association of plasminogen deficiency with aHUS. This adds to the emerging view that factors unrelated to the complement system can also be central to aHUS pathogenesis and suggests that future research on the mechanism of the disease should expand beyond complement dysregulation.
authors.affiliation: Department of Bacteriology and Immunology, and Research Programs Unit, Immunobiology, University of Helsinki, Helsinki, Finland.
authors.forename: Satu
authors.initials: S
authors.lastname: Hyvärinen
authors.affiliation: Department of Bacteriology and Immunology, and Research Programs Unit, Immunobiology, University of Helsinki, Helsinki, Finland.
authors.forename: T Sakari
authors.initials: TS
authors.lastname: Jokiranta
mesh_terms: D001792:Blood Platelets / Q000378:metabolism* / Q000473:pathology; D003167:Complement Activation; D004912:Erythrocytes / Q000378:metabolism* / Q000473:pathology; D005260:Female; D005341:Fibrinolysin / Q000378:metabolism*; D006461:Hemolysis; D006463:Hemolytic-Uremic Syndrome / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D008297:Male; D010958:Plasminogen / Q000378:metabolism*; D010974:Platelet Aggregation; D059748:Proteolysis
chemical_list: D010958:Plasminogen; D005341:Fibrinolysin