title: Highly sensitive electrochemical sensing platform for lead ion based on synergetic catalysis of DNAzyme and Au-Pd porous bimetallic nanostructures.
abstract: In this work, a sensitive and specific electrochemical biosensor for lead ion (Pb(2+)) detection was developed by coupling with synergetic catalysis of porous Au-Pd bimetallic nanoparticles and hemin/G-quadruplex-based DNAzyme. In the presence of target Pb(2+), the substrate strand was cleaved and the enzyme strand was released. Subsequently, G-rich DNA-labeled Au-Pd bimetallic nanoparticle was linked with the released enzyme strand through the helper DNA. Upon addition of hemin, a large number of hemin/G-quadruplex-based DNAzyme molecules were formed on the electrode to serve as nicotinamide adenine dinucleotide hydrogen (NADH) oxidase and peroxidase mimics. DNAzyme could catalyzed the reduction of H2O2, generated from NADH in the presence of O2, to produce an electrochemical signal when using thionine as the electron mediator. Introduction of porors Au-Pd bimetallic nanoparticles could enhance the detectable signal and cause the increase in the sensitivity. Experimental results showed that the variations (∆I) in the cathodic peak currents of the biosensor were linearly dependent on target Pb(2+) concentrations from 1.0 pM to 100 nM with a detection limit (LOD) of 0.34 pM. The excellent performance of the sensing platform indicated its promising prospect as a valuable tool for simple and cost-effective Pb(2+) detection in practical application.
authors.affiliation: Key Laboratory of Analysis and Detection for Food Safety (Ministry of Education & Fujian Province), Institute of Nanomedicine and Nanobiosensing, Department of Chemistry, Fuzhou University, Fuzhou 350108, PR China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Analysis and Detection for Food Safety (Ministry of Education & Fujian Province), Institute of Nanomedicine and Nanobiosensing, Department of Chemistry, Fuzhou University, Fuzhou 350108, PR China.
authors.forename: Youxiu
authors.initials: Y
authors.lastname: Lin
authors.affiliation: Key Laboratory of Analysis and Detection for Food Safety (Ministry of Education & Fujian Province), Institute of Nanomedicine and Nanobiosensing, Department of Chemistry, Fuzhou University, Fuzhou 350108, PR China.
authors.forename: Yuping
authors.initials: Y
authors.lastname: Lin
authors.affiliation: Key Laboratory of Analysis and Detection for Food Safety (Ministry of Education & Fujian Province), Institute of Nanomedicine and Nanobiosensing, Department of Chemistry, Fuzhou University, Fuzhou 350108, PR China. Electronic address: qhw76@fzu.edu.cn.
authors.forename: Qiaohua
authors.initials: Q
authors.lastname: Wei
authors.affiliation: Key Laboratory of Analysis and Detection for Food Safety (Ministry of Education & Fujian Province), Institute of Nanomedicine and Nanobiosensing, Department of Chemistry, Fuzhou University, Fuzhou 350108, PR China.
authors.forename: Guonan
authors.initials: G
authors.lastname: Chen
authors.affiliation: Key Laboratory of Analysis and Detection for Food Safety (Ministry of Education & Fujian Province), Institute of Nanomedicine and Nanobiosensing, Department of Chemistry, Fuzhou University, Fuzhou 350108, PR China. Electronic address: dianping.tang@fzu.edu.cn.
authors.forename: Dianping
authors.initials: D
authors.lastname: Tang
mesh_terms: D015374:Biosensing Techniques; D002384:Catalysis; D021881:DNA, Catalytic / Q000737:chemistry; D055664:Electrochemical Techniques / Q000379:methods*; D054856:G-Quadruplexes; D006046:Gold / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000737:chemistry; D007854:Lead / Q000737:chemistry / Q000302:isolation & purification*; D057230:Limit of Detection; D049329:Nanostructures / Q000737:chemistry*; D010165:Palladium / Q000737:chemistry
chemical_list: D021881:DNA, Catalytic; D007854:Lead; D010165:Palladium; D006046:Gold; D006861:Hydrogen Peroxide
keywords: Bimetallic nanoparticles; DNAzyme; Electrochemical biosensor; Lead ion