title: miR-215 functions as a tumor suppressor and directly targets ZEB2 in human non-small cell lung cancer.
abstract: MicroRNA-215 (miR-215) has previously been demonstrated to be dysregulated in a number of human malignancies and to be correlated with tumor progression. However, the expression and function of miR-215 in non-small cell lung cancer (NSCLC) has remained to be elucidated. Therefore, the present study aimed to investigate the effects of miR-215 in NSCLC tumorigenesis and development. Reverse transcription-quantitative polymerase chain reaction was used to evaluate miR-215 expression in NSCLC cell lines and primary tumor tissues. The association between miR-215 expression and certain clinicopathological factors was also determined, and the effects of miR-215 on the biological behavior of NSCLC cells were investigated. In addition, the potential regulatory function of miR-215 on zinc finger E-box-binding homeobox 2 (ZEB2) expression was examined. miR-215 expression was significantly downregulated in NSCLC cell lines and clinical specimens. Reduced miR-215 expression was significantly associated with lymph node metastasis and advanced TNM stage. Overexpression of miR-215 inhibited NSCLC cell proliferation, invasion and migration, and promoted cell apoptosisin vitro, and suppressed tumorigenicityin vivo. Furthermore, luciferase reporter assay analysis identified ZEB2 as a direct target of miR-215. These findings indicated that miR-215 may act as a tumor suppressor in NSCLC and may serve as a novel therapeutic agent for miR-based therapy.
authors.affiliation: Department of Pediatrics, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, P.R. China ; Department of Pediatrics, Central Hospital, Xiangyang, Hubei 441021, P.R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Hou
authors.affiliation: Department of Pediatrics, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, P.R. China.
authors.forename: Junwen
authors.initials: J
authors.lastname: Zhen
authors.affiliation: Department of Pediatrics, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, P.R. China.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Xu
authors.affiliation: Department of Pediatrics, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, P.R. China.
authors.forename: Kun
authors.initials: K
authors.lastname: Zhen
authors.affiliation: Department of Pediatrics, Central Hospital, Xiangyang, Hubei 441021, P.R. China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhu
authors.affiliation: Department of Pediatrics, Central Hospital, Xiangyang, Hubei 441021, P.R. China.
authors.forename: Rui
authors.initials: R
authors.lastname: Pan
authors.affiliation: Department of Pediatrics, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, P.R. China.
authors.forename: Chidong
authors.initials: C
authors.lastname: Zhao
keywords: invasion; miR-215; non-small cell lung cancer; proliferation; zinc finger E-box-binding homeobox 2