title: Enzymatic monitoring of lignin and lignin derivatives biooxidation.
abstract: Lignin oxidation was enzymatically monitored by measuring methanol released during the reaction. The methanol was oxidized to formaldehyde and hydrogen peroxide, and the latter used to oxidize ABTS to a product measured spectrophotometrically. The efficiency was comparable to the commonly used gas chromatography method. The assay was fast and inexpensive.
authors.affiliation: Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, P.O. Box 124, SE-221 00 Lund, Sweden.
authors.forename: Victor
authors.initials: V
authors.lastname: Ibrahim
authors.affiliation: Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, P.O. Box 124, SE-221 00 Lund, Sweden. Electronic address: gashaw.mamo@biotek.lu.se.
authors.forename: Gashaw
authors.initials: G
authors.lastname: Mamo
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D002849:Chromatography, Gas / Q000379:methods; D057075:Enzyme Assays / Q000379:methods*; D005557:Formaldehyde / Q000032:analysis / Q000737:chemistry; D006735:Horseradish Peroxidase / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000737:chemistry; D042845:Laccase / Q000032:analysis / Q000737:chemistry; D008031:Lignin / Q000737:chemistry / Q000378:metabolism*; D000432:Methanol / Q000032:analysis / Q000737:chemistry; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000737:chemistry / Q000378:metabolism; D013053:Spectrophotometry / Q000379:methods
chemical_list: D005557:Formaldehyde; D008031:Lignin; D006861:Hydrogen Peroxide; D010088:Oxidoreductases; D042845:Laccase; D006735:Horseradish Peroxidase; D000432:Methanol
keywords: Gas chromatography; Horse radish peroxidase; Laccase; Lignin; Methanol determination