title: An Ex vivo culture model for placental cytomegalovirus infection using slices of Guinea pig placental tissue.
abstract: Congenital infection with human cytomegalovirus (CMV) through the placenta is one of the major causes of birth and developmental abnormalities. Guinea pig CMV (GPCMV) causes in utero infection, which makes its animal models useful for studies on congenital diseases. Here, we established an ex vivo culture method for tissue slices prepared from guinea pig placentas and demonstrated that viral spread in the model resembles those in the placenta of GPCMV-infected animals and that the infection is independent of the pentameric glycoprotein complex for endothelial/epithelial cell tropism. Thus, this model affords a useful tool for pathobiological studies on CMV placental infection.
authors.affiliation: Department of Virology I, National Institute of Infectious Diseases, Tokyo, Japan. Electronic address: syamada@niid.go.jp.
authors.forename: Souichi
authors.initials: S
authors.lastname: Yamada
authors.affiliation: Department of Pathology, National Institute of Infectious Diseases, Tokyo, Japan.
authors.forename: Harutaka
authors.initials: H
authors.lastname: Katano
authors.affiliation: Department of Pathology, National Institute of Infectious Diseases, Tokyo, Japan.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Sato
authors.affiliation: Department of Virology I, National Institute of Infectious Diseases, Tokyo, Japan.
authors.forename: Saki
authors.initials: S
authors.lastname: Fukuchi
authors.affiliation: Department of Virology I, National Institute of Infectious Diseases, Tokyo, Japan.
authors.forename: Kaede
authors.initials: K
authors.lastname: Hashimoto
authors.affiliation: Department of Virology I, National Institute of Infectious Diseases, Tokyo, Japan; Department of Microbiology and Immunology, Gifu Pharmaceutical University, Gifu, Japan. Electronic address: inoue@gifu-pu.ac.jp.
authors.forename: Naoki
authors.initials: N
authors.lastname: Inoue
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D003586:Cytomegalovirus Infections / Q000151:congenital* / Q000473:pathology*; D004195:Disease Models, Animal; D005260:Female; D006168:Guinea Pigs; D018445:Infectious Disease Transmission, Vertical; D008962:Models, Theoretical; D009924:Organ Culture Techniques; D010920:Placenta / Q000473:pathology* / Q000821:virology; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000473:pathology*; D061251:Primary Cell Culture / Q000379:methods
keywords: Congenital infection; Cytomegalovirus; Guinea pig; Placenta; Slice culture