title: Widespread Neuronal Transduction of the Rodent CNS via Neonatal Viral Injection.
abstract: The rapid pace of neuroscience research demands equally efficient and flexible methods for genetically manipulating and visualizing selected neurons within the rodent brain. The use of viral vectors for gene delivery saves the time and cost of traditional germline transgenesis and offers the versatility of readily available reagents that can be easily customized to meet individual experimental needs. Here, we present a protocol for widespread neuronal transduction based on intraventricular viral injection of the neonatal mouse brain. Injections can be done either free-hand or assisted by a stereotaxic device to produce lifelong expression of virally delivered transgenes.
authors.affiliation: Department of Neuroscience, Baylor College of Medicine, Houston, TX, USA.
authors.forename: Ji-Yoen
authors.initials: JY
authors.lastname: Kim
authors.affiliation: Department of Neuroscience, Baylor College of Medicine, Houston, TX, USA.
authors.forename: Stacy D
authors.initials: SD
authors.lastname: Grunke
authors.affiliation: Department of Neuroscience, Baylor College of Medicine, Houston, TX, USA. jankowsk@bcm.edu.|Departments of Neurology and Neurosurgery, Huffington Center on Aging, Baylor College of Medicine, Houston, TX, USA. jankowsk@bcm.edu.
authors.forename: Joanna L
authors.initials: JL
authors.lastname: Jankowsky
mesh_terms: D000818:Animals; D000831:Animals, Newborn / Q000821:virology; D000229:Dependovirus / Q000235:genetics / Q000502:physiology*; D005822:Genetic Vectors / Q000008:administration & dosage; D007276:Injections, Intraventricular; D051379:Mice; D009474:Neurons / Q000378:metabolism*; D014161:Transduction, Genetic
keywords: Adeno-associated virus; Intracerebroventricular injection; Neonatal brain; Transgenic mouse; Viral transduction