title: The effect of protein supplement concentration in embryo transfer medium on clinical outcome of IVF/ICSI cycles: a prospective, randomized clinical trial.
abstract: The aim of this prospective, randomized clinical trial (RCT) was to evaluate whether the supplemental protein concentration in embryo transfer (ET) medium affects the clinical outcomes in IVF-ET. A total of 750 patients undergoing IVF-ET who met the inclusion criteria were randomly divided into three groups, according to the concentration of synthetic serum substitute (SSS) in ET medium as follows: 10% (Group A), 20% (Group B) and 50% (Group C). The patient characteristics and embryology data were all similar among the groups. The rates of implantation, clinical pregnancy and live birth were compared. Clinical pregnancy (44.61%, 48.79% and 45.49%), multiple pregnancy (24.18%, 28.71% and 25.0%), implantation (28.21%, 30.68% and 29.86%) and live birth (41.67%, 43.96% and 41.70%) rates in the three groups (A, B and C, respectively) showed no significant differences. This RCT demonstrates that supplemental protein concentration in the ET medium does not affect the treatment outcomes in IVF-ET. There was no statistical evidence to support the hypothesis that supplemental protein concentration in the ET medium influences treatment outcomes in IVF-ET.
authors.affiliation: Reproductive Health Center, Second Affiliated Hospital of Wenzhou Medical University, 109 Xueyuan Road, Wenzhou, Zhejiang Province, 32500, China.
authors.forename: Jianying
authors.initials: J
authors.lastname: Huang
authors.affiliation: Reproductive Health Center, Second Affiliated Hospital of Wenzhou Medical University, 109 Xueyuan Road, Wenzhou, Zhejiang Province, 32500, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Chen
authors.affiliation: Reproductive Health Center, Second Affiliated Hospital of Wenzhou Medical University, 109 Xueyuan Road, Wenzhou, Zhejiang Province, 32500, China.
authors.forename: Xiaosheng
authors.initials: X
authors.lastname: Lu
authors.affiliation: Reproductive Health Center, Second Affiliated Hospital of Wenzhou Medical University, 109 Xueyuan Road, Wenzhou, Zhejiang Province, 32500, China.
authors.forename: Xiaona
authors.initials: X
authors.lastname: Wang
authors.affiliation: Reproductive Health Center, Second Affiliated Hospital of Wenzhou Medical University, 109 Xueyuan Road, Wenzhou, Zhejiang Province, 32500, China.
authors.forename: HaiTao
authors.initials: H
authors.lastname: Xi
authors.affiliation: Reproductive Health Center, Second Affiliated Hospital of Wenzhou Medical University, 109 Xueyuan Road, Wenzhou, Zhejiang Province, 32500, China.
authors.forename: ChunFang
authors.initials: C
authors.lastname: Zhu
authors.affiliation: Reproductive Health Center, Second Affiliated Hospital of Wenzhou Medical University, 109 Xueyuan Road, Wenzhou, Zhejiang Province, 32500, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Reproductive Health Center, Second Affiliated Hospital of Wenzhou Medical University, 109 Xueyuan Road, Wenzhou, Zhejiang Province, 32500, China.
authors.forename: Jieqiang
authors.initials: J
authors.lastname: Lv
authors.affiliation: Reproductive Health Center, Second Affiliated Hospital of Wenzhou Medical University, 109 Xueyuan Road, Wenzhou, Zhejiang Province, 32500, China. Electronic address: dafeng76@126.com.
authors.forename: Hongshan
authors.initials: H
authors.lastname: Ge
mesh_terms: D000328:Adult; D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology*; D046149:Embryo Culture Techniques / Q000379:methods; D004624:Embryo Transfer / Q000379:methods*; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods / Q000706:statistics & numerical data; D006801:Humans; D008297:Male; D011247:Pregnancy; D011256:Pregnancy Outcome / Q000453:epidemiology; D011506:Proteins / Q000494:pharmacology*; D020554:Sperm Injections, Intracytoplasmic / Q000379:methods / Q000706:statistics & numerical data; D016896:Treatment Outcome
chemical_list: D003470:Culture Media; D011506:Proteins
keywords: clinical outcomes; prospective randomized controlled trial; serum albumin; transfer medium