title: A simple highly sensitive and selective aptamer-based colorimetric sensor for environmental toxins microcystin-LR in water samples.
abstract: A simple and highly sensitive aptamer-based colorimetric sensor was developed for selective detection of Microcystin-LR (MC-LR). The aptamer (ABA) was employed as recognition element which could bind MC-LR with high-affinity, while gold nanoparticles (AuNPs) worked as sensing materials whose plasma resonance absorption peaks red shifted upon binding of the targets at a high concentration of sodium chloride. With the addition of MC-LR, the random coil aptamer adsorbed on Au NPs altered into regulated structure to form MC-LR-aptamer complexes and broke away from the surface of Au NPs, leading to the aggregation of AuNPs, and the color converted from red to blue due to the interparticle plasmon coupling. Results showed that our aptamer-based colorimetric sensor exhibited rapid and sensitive detection performance for MC-LR with linear range from 0.5 nM to 7.5 μM and the detection limit reached 0.37 nM. Meanwhile, the pollutants usually coexisting with MC-LR in pollutant water samples had not demonstrated disturbance for detecting of MC-LR. The mechanism was also proposed suggesting that high affinity interaction between aptamer and MC-LR significantly enhanced the sensitivity and selectivity for MC-LR detection. Besides, the established method was utilized in analyzing real water samples and splendid sensitivity and selectivity were obtained as well.
authors.affiliation: Department of Chemistry, Tongji University, 1239 Siping Road, Shanghai 200092, People's Republic of China.
authors.forename: Xiuyan
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Chemistry, Tongji University, 1239 Siping Road, Shanghai 200092, People's Republic of China.
authors.forename: Ruojie
authors.initials: R
authors.lastname: Cheng
authors.affiliation: Department of Chemistry, Tongji University, 1239 Siping Road, Shanghai 200092, People's Republic of China.
authors.forename: Huijie
authors.initials: H
authors.lastname: Shi
authors.affiliation: Department of Chemistry, Tongji University, 1239 Siping Road, Shanghai 200092, People's Republic of China.
authors.forename: Bo
authors.initials: B
authors.lastname: Tang
authors.affiliation: Department of Chemistry, Tongji University, 1239 Siping Road, Shanghai 200092, People's Republic of China.
authors.forename: Hanshuang
authors.initials: H
authors.lastname: Xiao
authors.affiliation: Department of Chemistry, Tongji University, 1239 Siping Road, Shanghai 200092, People's Republic of China. Electronic address: g.zhao@tongji.edu.cn.
authors.forename: Guohua
authors.initials: G
authors.lastname: Zhao
mesh_terms: D000327:Adsorption; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D003124:Colorimetry; D060766:Drinking Water / Q000032:analysis; D006046:Gold / Q000737:chemistry*; D008387:Marine Toxins; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D052998:Microcystins / Q000032:analysis* / Q000737:chemistry; D046529:Microscopy, Electron, Transmission; D061005:Ponds / Q000032:analysis; D014873:Water Pollutants / Q000032:analysis* / Q000737:chemistry
chemical_list: D052157:Aptamers, Nucleotide; D060766:Drinking Water; D008387:Marine Toxins; D052998:Microcystins; D014873:Water Pollutants; D006046:Gold; C057862:cyanoginosin LR
keywords: Aptamer; Colorimetric sensor; High selectivity; High sensitivity; Microcystin-LR (MC-LR)