title: A report on the outbreak of Zika virus on Easter Island, South Pacific, 2014.
abstract: Zika virus (ZIKV) is an emerging mosquito-borne flavivirus circulating in Asia and Africa. In 2013, a large outbreak was reported on the archipelago of French Polynesia. In this study, we report the detection and molecular characterization of Zika virus for the first time in Chile from an outbreak among the inhabitants of Easter Island. A total of 89 samples from patients suspected of having ZIKV infection were collected between the period from January to May, 2014. Molecular diagnosis of the virus was performed by RT-PCR followed by the sequencing of the region containing the NS5 gene. A comparison of the viral nucleic acid sequence with those of other strains of ZIKA virus was performed using the MEGA software. Fifty-one samples were found positive for ZIKV by RT-PCR analysis. Further analysis of the NS5 gene revealed that the ZIKV strains identified in Easter Island were most closely related to those found in French Polynesia (99.8 to 99.9% nt and 100% aa sequence identity). These results strongly suggest that the transmission pathway leading to the introduction of Zika virus on Easter Island has its origin in French Polynesia.
authors.affiliation: Subdepartment of Molecular Genetics, Public Health Institute of Chile, Santiago, Chile.
authors.forename: J
authors.initials: J
authors.lastname: Tognarelli
authors.affiliation: Subdepartment of Molecular Genetics, Public Health Institute of Chile, Santiago, Chile.
authors.forename: S
authors.initials: S
authors.lastname: Ulloa
authors.affiliation: Emergent and Hepatic Virus Laboratory, Public Health Institute of Chile, Santiago, Chile.
authors.forename: E
authors.initials: E
authors.lastname: Villagra
authors.affiliation: Subdepartment of Molecular Genetics, Public Health Institute of Chile, Santiago, Chile.
authors.forename: J
authors.initials: J
authors.lastname: Lagos
authors.affiliation: Subdepartment of Molecular Genetics, Public Health Institute of Chile, Santiago, Chile.
authors.forename: C
authors.initials: C
authors.lastname: Aguayo
authors.affiliation: Subdepartment of Virology, Public Health Institute of Chile, Santiago, Chile.
authors.forename: R
authors.initials: R
authors.lastname: Fasce
authors.affiliation: Subdepartment of Molecular Genetics, Public Health Institute of Chile, Santiago, Chile.
authors.forename: B
authors.initials: B
authors.lastname: Parra
authors.affiliation: Emergent and Hepatic Virus Laboratory, Public Health Institute of Chile, Santiago, Chile.
authors.forename: J
authors.initials: J
authors.lastname: Mora
authors.affiliation: Emergent and Hepatic Virus Laboratory, Public Health Institute of Chile, Santiago, Chile.
authors.forename: N
authors.initials: N
authors.lastname: Becerra
authors.affiliation: Emergent and Hepatic Virus Laboratory, Public Health Institute of Chile, Santiago, Chile.
authors.forename: N
authors.initials: N
authors.lastname: Lagos
authors.affiliation: Emergent and Hepatic Virus Laboratory, Public Health Institute of Chile, Santiago, Chile.
authors.forename: L
authors.initials: L
authors.lastname: Vera
authors.affiliation: Subdepartment of Molecular Genetics, Public Health Institute of Chile, Santiago, Chile.
authors.forename: B
authors.initials: B
authors.lastname: Olivares
authors.affiliation: Emergent and Hepatic Virus Laboratory, Public Health Institute of Chile, Santiago, Chile.
authors.forename: M
authors.initials: M
authors.lastname: Vilches
authors.affiliation: Subdepartment of Molecular Genetics, Public Health Institute of Chile, Santiago, Chile. jfernand@ispch.cl.
authors.forename: J
authors.initials: J
authors.lastname: Fernández
mesh_terms: D016000:Cluster Analysis; D004196:Disease Outbreaks; D006801:Humans; D017720:Molecular Epidemiology; D010802:Phylogeny; D011114:Polynesia / Q000453:epidemiology; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D000071244:Zika Virus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D000071243:Zika Virus Infection / Q000453:epidemiology*
chemical_list: D012367:RNA, Viral
keywords: Easter Island; Outbreak; South Pacific; ZIKV; Zika virus