title: Genetic Evaluation of E. coli Strains Isolated from Asymptomatic Children with Neurogenic Bladders.
abstract: This study was conducted to describe the genetic profiles of E. coli that colonize asymptomatic pediatric neurogenic bladders. E. coli was isolated from 25 of 80 urine samples. Patients were excluded if they presented with symptomatic urinary tract infection or received treatment with antibiotics in the preceding three months. Multiplex PCR was performed to determine E. coli phylotype (A, B1, B2, and D) and the presence of seven pathogenicity islands (PAIs) and 10 virulence factors (VFs). E. coli strains were predominantly of the B1 and B2 phylotype, with few strains in the A or D phylotype. The PAIs IV536, ICFT073, and IICFT073 had the highest prevalence: 76%, 64%, and 48%, respectively. The PAIs II536, IJ96, and IIJ96 were less prevalent: 28%, 20%, and 24%, respectively. The most prevalent VF was vat (40%), while the least prevalent VFs were sfa (8%) and iha (12%). None of the strains carried the VF fyuA, which is very common in uropathogenic E. coli (UPEC). The genetic profiles of E. coli in this cohort seem to be more similar to UPEC than to commensal E. coli. However, they appear to have reduced virulence potential that allows them to colonize asymptomatically.
authors.affiliation: Division of Pediatric Urology, Medical College of Wisconsin, Children's Hospital of Wisconsin, 999 North 92nd Street, Milwaukee, WI 53226, USA.
authors.forename: John
authors.initials: J
authors.lastname: Kryger
authors.affiliation: Department of Urology, University of Wisconsin, 1685 Highland Avenue, Madison, WI 53705, USA.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Burleigh
authors.affiliation: Division of Pediatric Urology, Medical College of Wisconsin, Children's Hospital of Wisconsin, 999 North 92nd Street, Milwaukee, WI 53226, USA.
authors.forename: Melissa
authors.initials: M
authors.lastname: Christensen
authors.affiliation: Department of Urology, University of Wisconsin, 1685 Highland Avenue, Madison, WI 53705, USA.
authors.forename: Walter
authors.initials: W
authors.lastname: Hopkins