title: Neutralizing Antibody Response and Antibody-Dependent Cellular Cytotoxicity in HIV-1-Infected Individuals from Guinea-Bissau and Denmark.
abstract: The development of therapeutic and prophylactic HIV vaccines for African countries is urgently needed, but the question of what immunogens to use needs to be answered. One approach is to include HIV envelope immunogens derived from HIV-positive individuals from a geographically concentrated epidemic with more limited viral genetic diversity for a region-based vaccine. To address if there is a basis for a regional selected antibody vaccine, we have screened two regionally separate cohorts from Guinea-Bissau and Denmark for neutralizing antibody activity and antibody-dependent cellular cytotoxicity (ADCC) against local and nonlocal circulating HIV-1 strains. The neutralizing activity did not demonstrate higher potential against local circulating strains according to geography and subtype determination, but the plasma from Danish individuals demonstrated significantly higher inhibitory activity than that from Guinea-Bissau individuals against both local and nonlocal virus strains. Interestingly, an opposite pattern was observed with ADCC activity, where Guinea-Bissau individual plasma demonstrated higher activity than Danish plasma and was specifically against the local circulating subtype. Thus, on basis of samples from these two cohorts, no local-specific neutralizing activity was detected, but a local ADCC response was identified in the Guinea-Bissau samples, suggesting potential use of regional immunogens for an ADCC-inducing vaccine.
authors.affiliation: 1 Virus Research and Development Laboratory, Department of Microbiological Diagnostics and Virology, Statens Serum Institut , Copenhagen, Denmark .
authors.forename: Marie
authors.initials: M
authors.lastname: Borggren
authors.affiliation: 1 Virus Research and Development Laboratory, Department of Microbiological Diagnostics and Virology, Statens Serum Institut , Copenhagen, Denmark .
authors.forename: Sanne Skov
authors.initials: SS
authors.lastname: Jensen
authors.affiliation: 2 Biomedical Department, Virology Unit, Institute of Tropical Medicine , Antwerp, Belgium .
authors.forename: Leo
authors.initials: L
authors.lastname: Heyndrickx
authors.affiliation: 3 Department of Experimental Medical Science, Lund University , Lund, Sweden .
authors.forename: Angelica A
authors.initials: AA
authors.lastname: Palm
authors.affiliation: 4 Department of Infectious Diseases and Rheumatology, Copenhagen University Hospital , Copenhagen, Denmark .
authors.forename: Jan
authors.initials: J
authors.lastname: Gerstoft
authors.affiliation: 5 Department of Infectious Diseases, Copenhagen University Hospital , Hvidovre, Denmark .
authors.forename: Gitte
authors.initials: G
authors.lastname: Kronborg
authors.affiliation: 6 Bandim Health Project , Indepth Network, Bissau, Guinea-Bissau.|7 Department of Infectious Diseases, Aarhus University Hospital , Aarhus, Denmark .|8 Department of Clinical Immunology, Aarhus University Hospital , Aarhus, Denmark .
authors.forename: Bo Langhoff
authors.initials: BL
authors.lastname: Hønge
authors.affiliation: 6 Bandim Health Project , Indepth Network, Bissau, Guinea-Bissau.
authors.forename: Sanne
authors.initials: S
authors.lastname: Jespersen
authors.affiliation: 9 National Public Health Laboratory , Bissau, Guinea-Bissau.
authors.forename: Zacarias José
authors.initials: ZJ
authors.lastname: da Silva
authors.affiliation: 1 Virus Research and Development Laboratory, Department of Microbiological Diagnostics and Virology, Statens Serum Institut , Copenhagen, Denmark .
authors.forename: Ingrid
authors.initials: I
authors.lastname: Karlsson
authors.affiliation: 1 Virus Research and Development Laboratory, Department of Microbiological Diagnostics and Virology, Statens Serum Institut , Copenhagen, Denmark .|10 Infectious Disease Research Unit, Clinical Institute, University of Southern Denmark , Odense, Denmark .
authors.forename: Anders
authors.initials: A
authors.lastname: Fomsgaard
mesh_terms: D016915:AIDS Vaccines / Q000276:immunology; D057134:Antibodies, Neutralizing / Q000097:blood / Q000276:immunology*; D000920:Antibody-Dependent Cell Cytotoxicity / Q000276:immunology*; D001483:Base Sequence / Q000235:genetics; D003718:Denmark; D005260:Female; D006169:Guinea-Bissau; D015483:HIV Antibodies / Q000097:blood / Q000276:immunology*; D015488:HIV Antigens / Q000276:immunology*; D015699:HIV Envelope Protein gp120 / Q000235:genetics / Q000276:immunology; D015658:HIV Infections / Q000276:immunology / Q000821:virology; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D008297:Male; D054299:env Gene Products, Human Immunodeficiency Virus / Q000235:genetics / Q000276:immunology
chemical_list: D016915:AIDS Vaccines; D057134:Antibodies, Neutralizing; D015483:HIV Antibodies; D015488:HIV Antigens; D015699:HIV Envelope Protein gp120; D054299:env Gene Products, Human Immunodeficiency Virus; C097729:gp140 envelope protein, Human immunodeficiency virus 1