title: AsHSP17, a creeping bentgrass small heat shock protein modulates plant photosynthesis and ABA-dependent and independent signalling to attenuate plant response to abiotic stress.
abstract: Heat shock proteins (HSPs) are molecular chaperones that accumulate in response to heat and other abiotic stressors. Small HSPs (sHSPs) belong to the most ubiquitous HSP subgroup with molecular weights ranging from 12 to 42 kDa. We have cloned a new sHSP gene, AsHSP17 from creeping bentgrass (Agrostis stolonifera) and studied its role in plant response to environmental stress. AsHSP17 encodes a protein of 17 kDa. Its expression was strongly induced by heat in both leaf and root tissues, and by salt and abscisic acid (ABA) in roots. Transgenic Arabidopsis plants constitutively expressing AsHSP17 exhibited enhanced sensitivity to heat and salt stress accompanied by reduced leaf chlorophyll content and decreased photosynthesis under both normal and stressed conditions compared to wild type. Overexpression of AsHSP17 also led to hypersensitivity to exogenous ABA and salinity during germination and post-germinative growth. Gene expression analysis indicated that AsHSP17 modulates expression of photosynthesis-related genes and regulates ABA biosynthesis, metabolism and ABA signalling as well as ABA-independent stress signalling. Our results suggest that AsHSP17 may function as a protein chaperone to negatively regulate plant responses to adverse environmental stresses through modulating photosynthesis and ABA-dependent and independent signalling pathways.
authors.affiliation: Turfgrass Research Institute, Beijing Forestry University, Beijing, 100083, China.|Department of Genetics and Biochemistry, Clemson University, 110 Biosystems Research Complex, Clemson, SC, 29634, USA.|Key Laboratory of Crop Growth Regulation of Hebei Province, Agricultural University of Hebei, Baoding, 071001, China.
authors.forename: Xinbo
authors.initials: X
authors.lastname: Sun
authors.affiliation: College of Life Science, Jilin Agricultural University, Changchun, Jilin, 130118, China.|Department of Genetics and Biochemistry, Clemson University, 110 Biosystems Research Complex, Clemson, SC, 29634, USA.
authors.forename: Chunyu
authors.initials: C
authors.lastname: Sun
authors.affiliation: Department of Genetics and Biochemistry, Clemson University, 110 Biosystems Research Complex, Clemson, SC, 29634, USA.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Genetics and Biochemistry, Clemson University, 110 Biosystems Research Complex, Clemson, SC, 29634, USA.
authors.forename: Qian
authors.initials: Q
authors.lastname: Hu
authors.affiliation: Turfgrass Research Institute, Beijing Forestry University, Beijing, 100083, China.
authors.forename: Liebao
authors.initials: L
authors.lastname: Han
authors.affiliation: Department of Genetics and Biochemistry, Clemson University, 110 Biosystems Research Complex, Clemson, SC, 29634, USA.
authors.forename: Hong
authors.initials: H
authors.lastname: Luo
mesh_terms: D000040:Abscisic Acid / Q000502:physiology*; D031703:Agrostis / Q000235:genetics / Q000502:physiology*; D017360:Arabidopsis; D002734:Chlorophyll / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000502:physiology; D017343:Genes, Plant / Q000502:physiology; D006360:Heat-Shock Proteins / Q000235:genetics / Q000502:physiology*; D010788:Photosynthesis / Q000502:physiology*; D018515:Plant Leaves / Q000502:physiology; D010940:Plant Proteins / Q000235:genetics / Q000502:physiology*; D030821:Plants, Genetically Modified; D015398:Signal Transduction / Q000502:physiology; D013312:Stress, Physiological / Q000502:physiology
chemical_list: D006360:Heat-Shock Proteins; D010940:Plant Proteins; D002734:Chlorophyll; D000040:Abscisic Acid
keywords: ABA; AsHSP17; RNA-seq; abiotic stress; creeping bentgrass; salt tolerance; small heat shock protein