title: Moutan cortex extract exerts protective effects in a rat model of cardiac ischemia/reperfusion.
abstract: Moutan cortex (MC) is a traditional Chinese medicine with diverse biological effects. The present study was performed to investigate the effects of MC on myocardial ischemia/reperfusion (I/R) in rats and to explore its possible mechanisms. Sprague-Dawley rats were administered MC extract (1.98 g/kg, i.g.) for 14 days and underwent a subsequent open-chest procedure involving 30 min of myocardial ischemia and 60 min of reperfusion. The cardioprotective effect of MC was demonstrated by reduced infarct size and marked improvement in the histopathological examination. The increase in the activity of superoxide dismutase (SOD) and glutathione (GSH) as well as the reduction of malondialdehyde (MDA) indicated that MC effectively promoted the anti-oxidative defense system. Increased anti-oxidative defense was accompanied by decreased release of lactate dehydrogenase (LDH) and creatine kinase (CK). The reduction in TUNEL-positive myocytes demonstrated that MC decreased myocardial apoptosis. The mRNA expression of B cell leukemia-2 (Bcl-2) was upregulated by MC and the ratio of Bcl-2/Bcl-2-associated X protein (Bax) mRNA expression was increased. MC pretreatment decreased the mRNA expression of inducible nitric oxide synthase (iNOS). The data from this study suggest that MC exerted protective effects on acute myocardial I/R injury via anti-oxidative and anti-apoptotic activities.
authors.affiliation: a Key Laboratory of Chinese Medicine Resource and Compound Prescription (Hubei University of Chinese Medicine), Ministry of Education, 1 Huang-jia-hu, Wuhan, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Dan
authors.affiliation: a Key Laboratory of Chinese Medicine Resource and Compound Prescription (Hubei University of Chinese Medicine), Ministry of Education, 1 Huang-jia-hu, Wuhan, China.
authors.forename: Liping
authors.initials: L
authors.lastname: Zhang
authors.affiliation: a Key Laboratory of Chinese Medicine Resource and Compound Prescription (Hubei University of Chinese Medicine), Ministry of Education, 1 Huang-jia-hu, Wuhan, China.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Qin
authors.affiliation: a Key Laboratory of Chinese Medicine Resource and Compound Prescription (Hubei University of Chinese Medicine), Ministry of Education, 1 Huang-jia-hu, Wuhan, China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Peng
authors.affiliation: a Key Laboratory of Chinese Medicine Resource and Compound Prescription (Hubei University of Chinese Medicine), Ministry of Education, 1 Huang-jia-hu, Wuhan, China.
authors.forename: Mingyan
authors.initials: M
authors.lastname: Wong
authors.affiliation: b Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1277 Jiefang Avenue, Wuhan, China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Tan
authors.affiliation: c Arkansas Children's Nutrition Center, Little Rock, AR 72202, USA.
authors.forename: Shanggong
authors.initials: S
authors.lastname: Yu
authors.affiliation: c Arkansas Children's Nutrition Center, Little Rock, AR 72202, USA.|d Departments of Pediatrics, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA.
authors.forename: Nianbai
authors.initials: N
authors.lastname: Fang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D003402:Creatine Kinase / Q000378:metabolism; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005978:Glutathione / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D017202:Myocardial Ischemia / Q000188:drug therapy* / Q000378:metabolism; D015428:Myocardial Reperfusion Injury / Q000188:drug therapy* / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism; D032383:Myocytes, Cardiac / Q000187:drug effects / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D029596:Paeonia; D010936:Plant Extracts / Q000494:pharmacology*; D020011:Protective Agents / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; D020011:Protective Agents; D012333:RNA, Messenger; D051028:bcl-2-Associated X Protein; C038881:moutan cortex; D008315:Malondialdehyde; D007770:L-Lactate Dehydrogenase; D052247:Nitric Oxide Synthase Type II; D013482:Superoxide Dismutase; D003402:Creatine Kinase; D005978:Glutathione
keywords: Moutan cortex; anti-apoptose; anti-apoptotic; anti-oxidative; antioxydant; cardioprotecteur; cardioprotective; cortex Moutan; ischemia/reperfusion; ischémie/reperfusion