title: Identification and evaluation of the chemical similarity of Yindan xinnaotong samples by ultra high performance liquid chromatography with quadrupole time-of-flight mass spectrometry fingerprinting.
abstract: Yindan xinnaotong, a compound preparation used in traditional Chinese medicine, is composed of eight herbs: Ginkgo biloba leaf (yinxingye), Salvia miltiorrhizae (danshen), Herba gynostemmatis (jiaogulan), Erigerontis herba (dengzhanxixin), Allii sativi bulbus (dasuan), Notoginseng radixe rhizoma (sanqi), Crataegi fructus (shanzha), and Borneolum (tianranbingpian). Yindan xinnaotong is primarily used to treat cardiovascular and cerebrovascular diseases. However, to date, no scientific methods have been established to assess the quality of Yindan xinnaotong. Therefore, a combinatorial method was developed based on chemical constituent identification and fingerprint analysis to assess the consistency of Yindan xinnaotong quality. In this study, ultra high performance liquid chromatography coupled with time-of-flight mass spectrometry was used to identify the chemical components of Yindan xinnaotong soft capsules. Approximately 74 components were detected, of which 70, including flavonoids, ginkgolide, phenolic acid, diterpenoid tanshinones, and ginsenoside, were tentatively identified. A fingerprint analysis was also conducted to evaluate the uniformity of the quality of Yindan xinnaotong soft capsules. Ten batches of Yindan xinnaotong soft capsules were analyzed. All of the resulting chromatograms were imported into the "Similarity Evaluation System for Chromatographic Fingerprints of TCM" (Chinese Pharmacopoeia Commission, version 2004A). The similarity scores of common peaks from these samples ranged from 0.903-1.000, indicating that samples from different batches were highly correlated.
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Science, Beijing, P. R. China.
authors.forename: Leilei
authors.initials: L
authors.lastname: Gong
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Science, Beijing, P. R. China.
authors.forename: Xu
authors.initials: X
authors.lastname: Haiyu
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Science, Beijing, P. R. China.
authors.forename: Lan
authors.initials: L
authors.lastname: Wang
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Science, Beijing, P. R. China.
authors.forename: Yin
authors.initials: Y
authors.lastname: Xiaojie
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Science, Beijing, P. R. China.|School of Traditional Chinese Medicine, Capital Medical University, Beijing, P. R. China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Huijun
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Science, Beijing, P. R. China.|School of Traditional Chinese Medicine, Capital Medical University, Beijing, P. R. China.
authors.forename: Wang
authors.initials: W
authors.lastname: Songsong
authors.affiliation: Key Laboratory of Bioactive Substance and Resource Utilisation of the Chinese Herbal Medicine Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, P. R. China.
authors.forename: Long
authors.initials: L
authors.lastname: Cheng
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Science, Beijing, P. R. China.
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Ma
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Science, Beijing, P. R. China.
authors.forename: Shuangrong
authors.initials: S
authors.lastname: Gao
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Science, Beijing, P. R. China.
authors.forename: Rixin
authors.initials: R
authors.lastname: Liang
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Science, Beijing, P. R. China.
authors.forename: Hongjun
authors.initials: H
authors.lastname: Yang
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D008516:Medicine, Chinese Traditional; D018696:Neuroprotective Agents / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D004365:Drugs, Chinese Herbal; D018696:Neuroprotective Agents; C528303:Xinnao Shutong
keywords: Fingerprint analysis; Similarity evaluation; Traditional Chinese medicine; Yindan xinnaotong