title: Enhanced production of phenazine-like metabolite produced by Streptomyces aurantiogriseus VSMGT1014 against rice pathogen, Rhizoctonia solani.
abstract: The efficacy of a rhizobacterium Streptomyces aurantiogriseus VSMGT1014 for the production of bioactive metabolites with antifungal properties was evaluated under in vitro conditions. The production of bioactive metabolites by S. aurantiogriseus VSMGT1014 in International Streptomyces Project-2 (ISP-2) broth, supplemented with glucose and ammonium acetate was found to be the most suitable carbon and nitrogen sources for the maximum production of bioactive metabolites against rice pathogen, Rhizoctonia solani. The zone of inhibition range from 23.5 to 28.5 mm and 10.3 to 18.3 mm for glucose and ammonium acetate supplemented media, respectively. The culture filtrate of S. aurantiogriseus VSMGT1014 at pH 7.5, 37 °C at 120 rpm in 6 days of incubation showed the maximum production of bioactive metabolites with antagonistic potential. The crude metabolite was characterized by different spectral studies such as Ultraviolet spectrum, infrared-spectrum and based on the different analytical techniques, including thin layer chromatography (TLC) and high performance liquid chromatography (HPLC) with the retention time 29.4 and the bioactive metabolite was identified as phenazine, which was confirmed by pure phenazine compound as positive control.
authors.affiliation: Department of Microbial Technology, School of Biological Sciences,, Madurai Kamaraj University, Tamil Nadu, India.
authors.forename: Hariharan
authors.initials: H
authors.lastname: Harikrishnan
authors.affiliation: Department of Microbial Technology, School of Biological Sciences,, Madurai Kamaraj University, Tamil Nadu, India.|Department of Biology, Rutgers University, Camden, NJ, USA.
authors.forename: Vellasamy
authors.initials: V
authors.lastname: Shanmugaiah
authors.affiliation: Department of Microbial Technology, School of Biological Sciences,, Madurai Kamaraj University, Tamil Nadu, India.
authors.forename: Karmegham
authors.initials: K
authors.lastname: Nithya
authors.affiliation: UCIBIO and Department of Life Sciences, Faculty of Science and Technology, New University of Lisbon, Caparica, Portugal.
authors.forename: Natesan
authors.initials: N
authors.lastname: Balasubramanian
authors.affiliation: Microbiology Section, ICAR-Directorate of Soybean Research (ICAR-DSR Indore), Indore, India.
authors.forename: Mahaveer P
authors.initials: MP
authors.lastname: Sharma
authors.affiliation: Department of Biology, Rutgers University, Camden, NJ, USA.|Center for Computational and Integrative Biology, Rutgers University, Camden, NJ, USA.
authors.forename: Emma W
authors.initials: EW
authors.lastname: Gachomo
authors.affiliation: Department of Biology, Rutgers University, Camden, NJ, USA.|Center for Computational and Integrative Biology, Rutgers University, Camden, NJ, USA.
authors.forename: Simeon O
authors.initials: SO
authors.lastname: Kotchoni
mesh_terms: D000085:Acetates / Q000378:metabolism; D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D005947:Glucose / Q000378:metabolism; D012275:Oryza / Q000382:microbiology*; D010619:Phenazines / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D010935:Plant Diseases / Q000382:microbiology*; D012232:Rhizoctonia / Q000187:drug effects*; D013057:Spectrum Analysis; D013302:Streptomyces / Q000378:metabolism*; D013696:Temperature
chemical_list: D000085:Acetates; D000935:Antifungal Agents; D003470:Culture Media; D010619:Phenazines; C000598831:phenazine; D005947:Glucose; C018824:ammonium acetate
keywords: Antifungal metabolite; Phenazine; Rhizoctonia solani; Rice rhizosphere; Streptomyces aurantiogriseus