title: Identification of a warm-temperature acclimation-associated 65-kDa protein encoded by a temperature- and infection-responsive gene in the Kumgang fat minnow Rhynchocypris kumgangensis.
abstract: Water temperature is one of the most important factors in fish physiology; thus, it is important to identify genes that respond to changes in water temperature. In this study, we identified a warm- temperature acclimation-associated 65-kDa protein (Wap65) in the Kumgang fat minnow Rhynchocypris kumgangensis, a small, cold-freshwater fish species endemic to Korea. Kumgang fat minnow Wap65-1 (kmWap65-1) was cloned using polymerase chain reaction (PCR)-based strategies, and was found to be highly homologous with teleost Wap65-1 and mammalian hemopexin, a heme-binding protein that transfers plasma heme into hepatocytes. kmWap65-1 mRNA was expressed mainly in the liver and its expression levels were significantly increased by both short- and long-term exposure to high temperature, which was evaluated by real-time quantitative PCR. Furthermore, the expression levels of kmWap65-1 were highly elevated by exposure to bacterial lipopolysaccharide. These results indicate that kmWap65-1 expression is associated with environmental stresses such as increases in water temperature and bacterial infection. J. Exp. Zool. 325A:65-74, 2016. © 2015 Wiley Periodicals, Inc.
authors.affiliation: Department of Life Science, Kyonggi University, Suwon, Republic of Korea.
authors.forename: Jisu
authors.initials: J
authors.lastname: Im
authors.affiliation: Department of Life Science, Kyonggi University, Suwon, Republic of Korea.
authors.forename: Gibeom
authors.initials: G
authors.lastname: Kwon
authors.affiliation: Department of Life Science, Kyonggi University, Suwon, Republic of Korea.
authors.forename: Dongsoo
authors.initials: D
authors.lastname: Kong
authors.affiliation: Department of Life Science, Kyonggi University, Suwon, Republic of Korea.
authors.forename: Sungho
authors.initials: S
authors.lastname: Ghil
mesh_terms: D000064:Acclimatization / Q000235:genetics*; D000818:Animals; D003530:Cyprinidae / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D029941:Fish Proteins; D006466:Hemopexin / Q000235:genetics; D006358:Hot Temperature; D010802:Phylogeny; D011506:Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D029941:Fish Proteins; D011506:Proteins; D012333:RNA, Messenger; D006466:Hemopexin