title: Progression of inflammation during immunodeficient mouse skeletal muscle regeneration.
abstract: The skeletal muscle injury triggers the inflammatory response which is crucial for damaged muscle fiber degradation and satellite cell activation. Immunodeficient mice are often used as a model to study the myogenic potential of transplanted human stem cells. Therefore, it is crucial to elucidate whether such model truly reflects processes occurring under physiological conditions. To answer this question we compared skeletal muscle regeneration of BALB/c, i.e. animals producing all types of inflammatory cells, and SCID mice. Results of our study documented that initial stages of muscles regeneration in both strains of mice were comparable. However, lower number of mononucleated cells was noticed in regenerating SCID mouse muscles. Significant differences in the number of CD14-/CD45+ and CD14+/CD45+ cells between BALB/c and SCID muscles were also observed. In addition, we found important differences in M1 and M2 macrophage levels of BALB/c and SCID mouse muscles identified by CD68 and CD163 markers. Thus, our data show that differences in inflammatory response during muscle regeneration, were not translated into significant modifications in muscle regeneration.
authors.affiliation: Department of Cytology, Faculty of Biology, University of Warsaw, Miecznikowa 1 St., 02-096, Warsaw, Poland.
authors.forename: Iwona
authors.initials: I
authors.lastname: Grabowska
authors.affiliation: Department of Cytology, Faculty of Biology, University of Warsaw, Miecznikowa 1 St., 02-096, Warsaw, Poland.
authors.forename: Magdalena A
authors.initials: MA
authors.lastname: Mazur
authors.affiliation: Department of Cytology, Faculty of Biology, University of Warsaw, Miecznikowa 1 St., 02-096, Warsaw, Poland.
authors.forename: K
authors.initials: K
authors.lastname: Kowalski
authors.affiliation: Department of Cytology, Faculty of Biology, University of Warsaw, Miecznikowa 1 St., 02-096, Warsaw, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Helinska
authors.affiliation: Department of Cytology, Faculty of Biology, University of Warsaw, Miecznikowa 1 St., 02-096, Warsaw, Poland.
authors.forename: Jerzy
authors.initials: J
authors.lastname: Moraczewski
authors.affiliation: Department of Cytology, Faculty of Biology, University of Warsaw, Miecznikowa 1 St., 02-096, Warsaw, Poland.
authors.forename: Władysława
authors.initials: W
authors.lastname: Stremińska
authors.affiliation: Laboratory of Flow Cytometry, Medical Center of Postgraduate Education, Marymoncka 99/103 St., 01-813, Warsaw, Poland.
authors.forename: Grażyna
authors.initials: G
authors.lastname: Hoser
authors.affiliation: Laboratory of Flow Cytometry, Medical Center of Postgraduate Education, Marymoncka 99/103 St., 01-813, Warsaw, Poland.
authors.forename: Jerzy
authors.initials: J
authors.lastname: Kawiak
authors.affiliation: Department of Cytology, Faculty of Biology, University of Warsaw, Miecznikowa 1 St., 02-096, Warsaw, Poland.
authors.forename: Maria A
authors.initials: MA
authors.lastname: Ciemerych
authors.affiliation: Department of Cytology, Faculty of Biology, University of Warsaw, Miecznikowa 1 St., 02-096, Warsaw, Poland. edbrzoska@biol.uw.edu.pl.
authors.forename: Edyta
authors.initials: E
authors.lastname: Brzoska
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D007249:Inflammation / Q000378:metabolism / Q000473:pathology*; D008264:Macrophages / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D016513:Mice, SCID; D018482:Muscle, Skeletal / Q000378:metabolism / Q000473:pathology*; D009135:Muscular Diseases / Q000378:metabolism / Q000473:pathology; D012038:Regeneration / Q000502:physiology*
chemical_list: D015415:Biomarkers
keywords: Immunodeficiency; Inflammation; Muscle regeneration