title: RAB-10 Regulates Dendritic Branching by Balancing Dendritic Transport.
abstract: The construction of a large dendritic arbor requires robust growth and the precise delivery of membrane and protein cargoes to specific subcellular regions of the developing dendrite. How the microtubule-based vesicular trafficking and sorting systems are regulated to distribute these dendritic development factors throughout the dendrite is not well understood. Here we identify the small GTPase RAB-10 and the exocyst complex as critical regulators of dendrite morphogenesis and patterning in the C. elegans sensory neuron PVD. In rab-10 mutants, PVD dendritic branches are reduced in the posterior region of the cell but are excessive in the distal anterior region of the cell. We also demonstrate that the dendritic branch distribution within PVD depends on the balance between the molecular motors kinesin-1/UNC-116 and dynein, and we propose that RAB-10 regulates dendrite morphology by balancing the activity of these motors to appropriately distribute branching factors, including the transmembrane receptor DMA-1.
authors.affiliation: Howard Hughes Medical Institute, Department of Biology, Stanford University, Stanford, California, United States of America.
authors.forename: Caitlin A
authors.initials: CA
authors.lastname: Taylor
authors.affiliation: Howard Hughes Medical Institute, Department of Biology, Stanford University, Stanford, California, United States of America.
authors.forename: Jing
authors.initials: J
authors.lastname: Yan
authors.affiliation: Howard Hughes Medical Institute, Department of Biology, Stanford University, Stanford, California, United States of America.
authors.forename: Audrey S
authors.initials: AS
authors.lastname: Howell
authors.affiliation: Howard Hughes Medical Institute, Department of Biology, Stanford University, Stanford, California, United States of America.
authors.forename: Xintong
authors.initials: X
authors.lastname: Dong
authors.affiliation: Howard Hughes Medical Institute, Department of Biology, Stanford University, Stanford, California, United States of America.
authors.forename: Kang
authors.initials: K
authors.lastname: Shen
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics / Q000254:growth & development; D029742:Caenorhabditis elegans Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000096:biosynthesis / Q000235:genetics*; D003712:Dendrites / Q000235:genetics* / Q000378:metabolism; D004398:Dyneins / Q000235:genetics / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D016547:Kinesins / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D008565:Membrane Proteins / Q000096:biosynthesis / Q000235:genetics*; D055495:Neurogenesis / Q000235:genetics*; D021381:Protein Transport / Q000235:genetics; D020691:rab GTP-Binding Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D029742:Caenorhabditis elegans Proteins; D018797:Cell Cycle Proteins; C569907:DMA-1 protein, C elegans; D008565:Membrane Proteins; C502663:UNC-116 protein, C elegans; D004398:Dyneins; D016547:Kinesins; C510977:RAB-10 protein, C elegans; D020691:rab GTP-Binding Proteins