title: Determination of histamine in canned tuna by molecularly imprinted polymers-surface enhanced Raman spectroscopy.
abstract: We introduce a rapid, cost effective and reliable approach to determine histamine level in canned tuna. Molecularly imprinted polymers (MIPs) were synthesized as artificial antibodies towards histamine by utilizing the interaction between histamine and a functional monomer (methacrylic acid) to impress specific binding sites on polymer particles after polymerization. Polyvinyl chloride (PVC) was used to immobilize the MIPs, yielding a MIPs-PVC film that functioned as a recognition element to specifically separate histamine from tuna extract. A gold colloid solution served both as an eluting solvent to extract histamine from MIPs-PVC film and furnish a substrate for surface enhanced Raman spectroscopy (SERS) detection of histamine signals. Principal component analysis together with a partial least square regression (PLSR) model (R(2) = 0.947, RMSECV = 3.526) verified the reliability of MIPs-PVC-SERS approach for the detection and spectral analysis of histamine. Linear regression models were also constructed to relate the intensity of different histamine SERS bands with the corresponding spiking levels. One such model (using a band at 1576 cm(-1)) performed slightly better in predicting histamine content in tuna than the PLSR model. We conclude that our new MIPs-PVC-SERS approach can rapidly and reliably determine histamine at levels from 3 to 90 ppm in canned tuna meat.
authors.affiliation: Food, Nutrition and Health Program, Faculty of Land and Food Systems, The University of British Columbia, Vancouver, British Columbia, V6T 1Z4, Canada; Department of Chemistry, The University of British Columbia, British Columbia, V6T 1Z1, Canada.
authors.forename: Fang
authors.initials: F
authors.lastname: Gao
authors.affiliation: Department of Chemistry, The University of British Columbia, British Columbia, V6T 1Z1, Canada.
authors.forename: Edward
authors.initials: E
authors.lastname: Grant
authors.affiliation: Food, Nutrition and Health Program, Faculty of Land and Food Systems, The University of British Columbia, Vancouver, British Columbia, V6T 1Z4, Canada. Electronic address: xiaonan.lu@ubc.ca.
authors.forename: Xiaonan
authors.initials: X
authors.lastname: Lu
mesh_terms: D000327:Adsorption; D000818:Animals; D005396:Fish Products / Q000032:analysis*; D006632:Histamine / Q000032:analysis*; D054802:Molecular Imprinting; D011108:Polymers / Q000737:chemistry*; D013059:Spectrum Analysis, Raman / Q000379:methods*; D013499:Surface Properties; D014413:Tuna
chemical_list: D011108:Polymers; D006632:Histamine
keywords: Fish; Food safety; Histamine; MIPs; SERS