title: Plant Cell-Based Recombinant Antibody Manufacturing with a 200 L Orbitally Shaken Disposable Bioreactor.
abstract: Tobacco BY-2 cells are an attractive platform for the manufacture of a variety of biopharmaceutical proteins, including antibodies. Here, we describe the scaled-up cultivation of human IgG-secreting BY-2 cells in a 200 L orbitally shaken disposable bioreactor, resulting in cell growth and recombinant protein yields that are proportionately comparable with those obtained from cultivations in 500 mL shake flasks. Furthermore, we present an efficient downstream process for antibody recovery from the viscous spent culture medium using expanded bed adsorption (EBA) chromatography.
authors.affiliation: Fraunhofer IME, Forckenbeckstraße 6, 52074, Aachen, Germany. nicole.raven@ime.fraunhofer.de.
authors.forename: Nicole
authors.initials: N
authors.lastname: Raven
authors.affiliation: Fraunhofer IME, Forckenbeckstraße 6, 52074, Aachen, Germany. stefan.schillberg@ime.fraunhofer.de.
authors.forename: Stefan
authors.initials: S
authors.lastname: Schillberg
authors.affiliation: Fraunhofer IME, Forckenbeckstraße 6, 52074, Aachen, Germany. stefan.rasche@ime.fraunhofer.de.
authors.forename: Stefan
authors.initials: S
authors.lastname: Rasche
mesh_terms: D019149:Bioreactors; D002845:Chromatography; D007074:Immunoglobulin G / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification; D059828:Plant Cells; D030821:Plants, Genetically Modified; D011994:Recombinant Proteins / Q000276:immunology / Q000302:isolation & purification; D014026:Nicotiana / Q000235:genetics* / Q000378:metabolism
chemical_list: D007074:Immunoglobulin G; D011994:Recombinant Proteins
keywords: Antibody purification; Expanded bed chromatography; Nicotiana tabacum Bright Yellow 2 (BY-2); Plant cell suspension culture; Scaled-up manufacture; Single-use technology