title: Rapid and sensitive detection of porcine torovirus by a reverse transcription loop-mediated isothermal amplification assay (RT-LAMP).
abstract: Porcine torovirus (PToV) is associated with swine gastroenteritis, but its pathogenesis is uncertain because there is limited information regarding PToV due to its difficulty to adapt in vitro. This study has developed a rapid one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) method for the detection of PToV. A set of four primers specific to six regions within the PToV's highly conserved fragment of the M gene was designed for use with the RT-LAMP assay. The RT-LAMP assay was sensitive with a detection limit of 1 × 10(1)copies/μL, which was 100-fold higher than reverse-transcription PCR. No cross-reaction was observed with other similar viruses. A total of 175 clinical specimens were collected from the Sichuan province, and PToV was detected by the established RT-LAMP assay with a positive rate of 39.2% (69/175). This study developed the first rapid, sensitive, simple, cost-effective and accurate method for the detection of PToV. The results show that the RT-LAMP assay is highly feasible in clinical settings.
authors.affiliation: Key Laboratory of Animal Biotechnology Center, College of Veterinary Medicine of Sichuan Agricultural University, Huimin Road 211, Wenjiang district, Sichuan 611134, PR China. Electronic address: abtclxw@126.com.
authors.forename: Xiaowan
authors.initials: X
authors.lastname: Liu
authors.affiliation: Key Laboratory of Animal Biotechnology Center, College of Veterinary Medicine of Sichuan Agricultural University, Huimin Road 211, Wenjiang district, Sichuan 611134, PR China. Electronic address: god098@163.com.
authors.forename: Yuancheng
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Animal Biotechnology Center, College of Veterinary Medicine of Sichuan Agricultural University, Huimin Road 211, Wenjiang district, Sichuan 611134, PR China. Electronic address: abtcyf@126.com.
authors.forename: Fan
authors.initials: F
authors.lastname: Yang
authors.affiliation: Key Laboratory of Animal Biotechnology Center, College of Veterinary Medicine of Sichuan Agricultural University, Huimin Road 211, Wenjiang district, Sichuan 611134, PR China. Electronic address: 1210695671@qq.com.
authors.forename: Pengjuan
authors.initials: P
authors.lastname: Liu
authors.affiliation: Key Laboratory of Animal Biotechnology Center, College of Veterinary Medicine of Sichuan Agricultural University, Huimin Road 211, Wenjiang district, Sichuan 611134, PR China. Electronic address: 429197524@qq.com.
authors.forename: Yuhan
authors.initials: Y
authors.lastname: Cai
authors.affiliation: Key Laboratory of Animal Biotechnology Center, College of Veterinary Medicine of Sichuan Agricultural University, Huimin Road 211, Wenjiang district, Sichuan 611134, PR China. Electronic address: 514570512@qq.com.
authors.forename: Jianbo
authors.initials: J
authors.lastname: Huang
authors.affiliation: Key Laboratory of Animal Biotechnology Center, College of Veterinary Medicine of Sichuan Agricultural University, Huimin Road 211, Wenjiang district, Sichuan 611134, PR China; Key Laboratory of Animal Diseases and Human Health of Sichuan Province, College of Veterinary Medicine of Sichuan Agricultural University, Huimin Road 211, Wenjiang District, Sichuan 611134, PR China. Electronic address: abtczl72@126.com.
authors.forename: Ling
authors.initials: L
authors.lastname: Zhu
authors.affiliation: Key Laboratory of Animal Biotechnology Center, College of Veterinary Medicine of Sichuan Agricultural University, Huimin Road 211, Wenjiang district, Sichuan 611134, PR China; Key Laboratory of Animal Diseases and Human Health of Sichuan Province, College of Veterinary Medicine of Sichuan Agricultural University, Huimin Road 211, Wenjiang District, Sichuan 611134, PR China. Electronic address: abtcxzw@126.com.
authors.forename: Zhiwen
authors.initials: Z
authors.lastname: Xu
mesh_terms: D000818:Animals; D003429:Cross Reactions; D017931:DNA Primers; D006801:Humans; D057230:Limit of Detection; D021141:Nucleic Acid Amplification Techniques / Q000191:economics / Q000379:methods*; D012367:RNA, Viral; D048348:Reverse Transcription; D012680:Sensitivity and Specificity; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis / Q000821:virology*; D017003:Torovirus / Q000235:genetics / Q000302:isolation & purification*; D018176:Torovirus Infections / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology
chemical_list: D017931:DNA Primers; D012367:RNA, Viral
keywords: Porcine torovirus; Rapid detection; Reverse transcription loop-mediated isothermal amplification assay