title: Transient Expression of Mammalian Genes in N. benthamiana to Modulate N-Glycosylation.
abstract: Nicotiana benthamiana has shown great success as a platform for the production of recombinant proteins. Here, we describe methods to transiently express high levels of recombinant proteins and simultaneously modulate their glycosylation pattern toward human-like structures. The method aims to generate recombinant proteins with a targeted largely homogeneous glycosylation profile for structure-function studies.
authors.affiliation: Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Muthgasse 18, 1190, Vienna, Austria.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Castilho
authors.affiliation: Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Muthgasse 18, 1190, Vienna, Austria. herta.steinkellner@boku.ac.at.
authors.forename: Herta
authors.initials: H
authors.lastname: Steinkellner
mesh_terms: D000818:Animals; D015870:Gene Expression; D018014:Gene Transfer Techniques; D016695:Glycosyltransferases / Q000235:genetics*; D006801:Humans; D008322:Mammals / Q000378:metabolism; D030821:Plants, Genetically Modified; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000235:genetics*; D014026:Nicotiana / Q000201:enzymology / Q000235:genetics*
chemical_list: D011994:Recombinant Proteins; D016695:Glycosyltransferases
keywords: Glycoengineering; Nicotiana benthamiana; Recombinant proteins; Transient expression