title: Bioethanol production from taro waste using thermo-tolerant yeast Kluyveromyces marxianus K21.
abstract: In the present study, evaluation and optimization of taro waste (TW), which was mainly composed of taro peels that contain many starch residues, as the main carbon source in medium were studied. The flask studies showed the optimal medium was using 170g/L of TW which is about 100g/L of glucose and 9g/L of CGM as alternative nitrogen source. Simultaneous saccharification and fermentation (SSF) exhibited higher bioethanol productivity toward separation hydrolysis and fermentation (SHF). The optimal condition of SSF was 5% of Kluyveromyces marxianus K21 inoculum at 40°C resulting in the maximum ethanol concentration (48.98g/L) and productivity (2.23g/L/h) after 22h of cultivation. The scaling up experiment in a 5L bioreactor demonstrated that K21 can still maintain its capability. After 20h of cultivation, 43.78g/L of ethanol (2.19g/L/h of productivity) was achieved corresponding to a 94.2% theoretical ethanol yield.
authors.affiliation: Graduate Institute of Food Science Technology, National Taiwan University, Taipei 10617, Taiwan.
authors.forename: Wei-Hao
authors.initials: WH
authors.lastname: Wu
authors.affiliation: Green Energy and Environment Research Laboratories, Industrial Technology Research Institute, Hsinchu 31040, Taiwan.
authors.forename: Wei-Chun
authors.initials: WC
authors.lastname: Hung
authors.affiliation: Department of Agricultural Chemistry, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei 10617, Taiwan.
authors.forename: Kai-Yin
authors.initials: KY
authors.lastname: Lo
authors.affiliation: Department of Food Science, Tunghai University, Taichung, Taiwan.
authors.forename: Yen-Hui
authors.initials: YH
authors.lastname: Chen
authors.affiliation: Green Energy and Environment Research Laboratories, Industrial Technology Research Institute, Hsinchu 31040, Taiwan.
authors.forename: Hou-Peng
authors.initials: HP
authors.lastname: Wan
authors.affiliation: Graduate Institute of Food Science Technology, National Taiwan University, Taipei 10617, Taiwan; Institute of Biotechnology, National Taiwan University, Taipei 10617, Taiwan. Electronic address: kccheng@ntu.edu.tw.
authors.forename: Kuan-Chen
authors.initials: KC
authors.lastname: Cheng
mesh_terms: D019149:Bioreactors; D031060:Colocasia / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D006868:Hydrolysis; D007716:Kluyveromyces / Q000254:growth & development / Q000378:metabolism*; D014866:Waste Products
chemical_list: D014866:Waste Products; D000431:Ethanol; D005947:Glucose
keywords: Alternative nitrogen source; Bioethanol; Kluyveromyces marxianus K21; Simultaneous saccharification fermentation; Taro waste