title: Substrate Interaction Networks of the Escherichia coli Chaperones: Trigger Factor, DnaK and GroEL.
abstract: In the dense cellular environment, protein misfolding and inter-molecular protein aggregation compete with protein folding. Chaperones associate with proteins to prevent misfolding and to assist in folding to the native state. In Escherichia coli, the chaperones trigger factor, DnaK/DnaJ/GrpE, and GroEL/ES are the major chaperones responsible for insuring proper de novo protein folding. With multitudes of proteins produced by the bacterium, the chaperones have to be selective for their substrates. Yet, chaperone selectivity cannot be too specific. Recent biochemical and high-throughput studies have provided important insights highlighting the strategies used by chaperones in maintaining proteostasis in the cell. Here, we discuss the substrate networks and cooperation among these protein folding chaperones.
authors.affiliation: Department of Biochemistry, University of Toronto, 1 King's College Circle, Medical Sciences Building, Room 5308, Toronto, ON, M5S 1A8, Canada.
authors.forename: Vaibhav
authors.initials: V
authors.lastname: Bhandari
authors.affiliation: Department of Biochemistry, University of Toronto, 1 King's College Circle, Medical Sciences Building, Room 5308, Toronto, ON, M5S 1A8, Canada. walid.houry@utoronto.ca.
authors.forename: Walid A
authors.initials: WA
authors.lastname: Houry
mesh_terms: D018835:Chaperonin 10 / Q000737:chemistry / Q000502:physiology; D018834:Chaperonin 60 / Q000502:physiology*; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000502:physiology*; D018840:HSP70 Heat-Shock Proteins / Q000737:chemistry / Q000502:physiology*; D006360:Heat-Shock Proteins / Q000737:chemistry / Q000502:physiology; D018832:Molecular Chaperones / Q000502:physiology*; D019696:Peptidylprolyl Isomerase / Q000737:chemistry / Q000502:physiology*; D017510:Protein Folding
chemical_list: D018835:Chaperonin 10; D018834:Chaperonin 60; D029968:Escherichia coli Proteins; C489400:GrpE protein, E coli; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; C063842:dnaK protein, E coli; C403324:trigger factor, E coli; D019696:Peptidylprolyl Isomerase
keywords: Chaperone interaction network; DnaK/DnaJ/GrpE; GroEL/GroES; Molecular chaperones; Protein aggregation; Protein folding; Trigger factor