title: Effect of Zinc on Appetite Regulatory Peptides in the Hypothalamus of Salmonella-Challenged Broiler Chickens.
abstract: The effects of dietary Zinc (Zn) supplementation on the gene expression of appetite regulatory peptides were investigated in Salmonella-infected broiler chickens. Broiler chickens (Arbor Acres, 1 day old) were allocated randomly into 24 pens of 10 birds. The chickens from 12 pens were fed with basal diet and the other with basal diet supplemented with Zn (ZnSO4·H2O, 120 mg/kg). At 5 days of age, the chickens were divided into 4 treatments with 6 pens: basal diet; basal diet and Salmonella challenge; Zn-supplemented diet; Zn-supplemented diet and Salmonella challenge. At 42 days of age, the hypothalamus from 6 chickens per treatment (1 chicken per pen) was individually collected for gene expression determination. Results showed that dietary supplementation of Zn reduced the gene expression of hypothalamic ghrelin and tumor necrosis factor alpha (TNF-α) (P < 0.05). Salmonella infection upregulated the messenger RNA (mRNA) levels of hypothalamic neuropeptide Y (NPY) and TNF-α. Zn supplementation and Salmonella inoculation were significantly correlated with the mRNA levels of toll-like receptor 2-1 (P < 0.05). However, neither dietary Zn supplementation nor Salmonella inoculation had significant effect on hypothalamic agouti-related protein, cocaine- and amphetamine-regulated transcript, and pro-opiomelanocortin. This study shows that dietary Zn supplementation promoted orexigenic appetite regulatory peptides and reduced the expression of the inflammatory cytokine TNF-α in the hypothalamus of Salmonella-challenged broilers.
authors.affiliation: Department of Animal Science, Shandong Agricultural University, Taian, Shandong, 271018, China.
authors.forename: Xiyi
authors.initials: X
authors.lastname: Hu
authors.affiliation: Department of Animal Science, Faculty of Agriculture, University of Kurdistan, Sanandaj, 66177-15175, Iran.
authors.forename: Ardashir
authors.initials: A
authors.lastname: Sheikhahmadi
authors.affiliation: Department of Animal Science, Shandong Agricultural University, Taian, Shandong, 271018, China.
authors.forename: Xianlei
authors.initials: X
authors.lastname: Li
authors.affiliation: Division Livestock-Nutrition-Quality, Department of Biosystems, KU Leuven, Kasteelpark Arenberg 30, 3001, Leuven, Belgium.
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Animal Science, Shandong Agricultural University, Taian, Shandong, 271018, China.
authors.forename: Hongchao
authors.initials: H
authors.lastname: Jiao
authors.affiliation: Department of Animal Science, Shandong Agricultural University, Taian, Shandong, 271018, China.
authors.forename: Hai
authors.initials: H
authors.lastname: Lin
authors.affiliation: College of Animal Science, China Agricultural University, Beijing, 100094, China.
authors.forename: Bingkun
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Department of Animal Science, Shandong Agricultural University, Taian, Shandong, 271018, China. naposong@qq.com.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Song
mesh_terms: D000818:Animals; D001066:Appetite / Q000187:drug effects* / Q000502:physiology; D002645:Chickens; D019587:Dietary Supplements; D007031:Hypothalamus / Q000187:drug effects* / Q000378:metabolism*; D008297:Male; D010455:Peptides / Q000235:genetics / Q000378:metabolism*; D011201:Poultry Diseases / Q000378:metabolism / Q000382:microbiology*; D012475:Salmonella / Q000502:physiology*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000235:genetics; D015032:Zinc / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D010455:Peptides; D014409:Tumor Necrosis Factor-alpha; D015032:Zinc
keywords: Appetite; Broiler; Hypothalamus; Salmonella typhimurium; Zinc