title: Molecular characterization and functional analysis of tumor necrosis factor receptor-associated factor 2 in the Pacific oyster.
abstract: Tumor necrosis factor receptor (TNFR)-associated factors (TRAFs) are a family of crucial adaptors, playing vital roles in mediating signal transduction in immune signaling pathways, including RIG-I-like receptor (RLR) signaling pathway. In the present study, a new TRAF family member (CgTRAF2) was identified in the Pacific oyster, Crassostrea gigas. Comparison and phylogenetic analysis revealed that CgTRAF2 could be a new member of the invertebrate TRAF2 family. Quantitative real-time PCR revealed that CgTRAF2 mRNA was highly expressed in the digestive gland, gills, and hemocytes, and it was significantly up-regulated after Vibrio alginolyticus and ostreid herpesvirus 1 (OsHV-1) challenge. The CgTRAF2 mRNA expression profile in different developmental stages of oyster larvae suggested that CgTRAF2 could function in early larval development. CgTRAF2 mRNA expression pattern, after the silence of CgMAVS (Mitochondrial Antiviral Signaling) -like, indicated that CgTRAF2 might function downstream of CgMAVS-like. Moreover, the subcellular localization analysis revealed that CgTRAF2 was localized in cytoplasm, and it may play predominately important roles in signal transduction. Collectively, these results demonstrated that CgTRAF2 might play important roles in the innate immunity and larval development of the Pacific oyster.
authors.affiliation: National & Local Joint Engineering Laboratory of Ecological Mariculture, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.
authors.forename: Baoyu
authors.initials: B
authors.lastname: Huang
authors.affiliation: National & Local Joint Engineering Laboratory of Ecological Mariculture, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.
authors.forename: Linlin
authors.initials: L
authors.lastname: Zhang
authors.affiliation: National & Local Joint Engineering Laboratory of Ecological Mariculture, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.
authors.forename: Yishuai
authors.initials: Y
authors.lastname: Du
authors.affiliation: National & Local Joint Engineering Laboratory of Ecological Mariculture, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China. Electronic address: lili@qdio.ac.cn.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: National & Local Joint Engineering Laboratory of Ecological Mariculture, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Xueying
authors.initials: X
authors.lastname: Tang
authors.affiliation: National & Local Joint Engineering Laboratory of Ecological Mariculture, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China. Electronic address: gfzhang@qdio.ac.cn.
authors.forename: Guofan
authors.initials: G
authors.lastname: Zhang
mesh_terms: D000818:Animals; D049896:Crassostrea / Q000235:genetics* / Q000382:microbiology / Q000821:virology; D018076:DNA, Complementary / Q000235:genetics; D041981:Gastrointestinal Tract / Q000378:metabolism; D005880:Gills / Q000378:metabolism; D006434:Hemocytes / Q000378:metabolism; D006564:Herpesviridae; D006566:Herpesviridae Infections / Q000235:genetics / Q000662:veterinary; D008969:Molecular Sequence Data; D010802:Phylogeny; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D047992:TNF Receptor-Associated Factor 2 / Q000235:genetics*; D015854:Up-Regulation; D014735:Vibrio Infections / Q000235:genetics / Q000662:veterinary; D044149:Vibrio alginolyticus
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger; D047992:TNF Receptor-Associated Factor 2
keywords: Crassostrea gigas; Innate immunity; MAVS; Pacific oyster; TRAF2