title: QUANTITY: An Isobaric Tag for Quantitative Glycomics.
abstract: Glycan is an important class of macromolecules that play numerous biological functions. Quantitative glycomics--analysis of glycans at global level--however, is far behind genomics and proteomics owing to technical challenges associated with their chemical properties and structural complexity. As a result, technologies that can facilitate global glycan analysis are highly sought after. Here, we present QUANTITY (Quaternary Amine Containing Isobaric Tag for Glycan), a quantitative approach that can not only enhance detection of glycans by mass spectrometry, but also allow high-throughput glycomic analysis from multiple biological samples. This robust tool enabled us to accomplish glycomic survey of bioengineered Chinese Hamster Ovary (CHO) cells with knock-in/out enzymes involved in protein glycosylation. Our results demonstrated QUANTITY is an invaluable technique for glycan analysis and bioengineering.
authors.affiliation: Department of Pathology, Johns Hopkins University, Baltimore, MD, USA.
authors.forename: Shuang
authors.initials: S
authors.lastname: Yang
authors.affiliation: Institute for Bioscience and Biotechnology Research, University of Maryland College Park, Rockville, MD, USA.|Analytical Biotechnology, MedImmune LLC, Gaithersburg, MD, USA.
authors.forename: Meiyao
authors.initials: M
authors.lastname: Wang
authors.affiliation: Department of Pathology, Johns Hopkins University, Baltimore, MD, USA.
authors.forename: Lijun
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD, USA.
authors.forename: Bojiao
authors.initials: B
authors.lastname: Yin
authors.affiliation: School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, China.
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Song
authors.affiliation: Institute for Bioscience and Biotechnology Research, University of Maryland College Park, Rockville, MD, USA.|Biomolecular Measurement Division, National Institute of Standard and Technology, Gaithersburg, MD, USA.
authors.forename: Illarion V
authors.initials: IV
authors.lastname: Turko
authors.affiliation: Biomolecular Measurement Division, National Institute of Standard and Technology, Gaithersburg, MD, USA.
authors.forename: Karen W
authors.initials: KW
authors.lastname: Phinney
authors.affiliation: Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD, USA.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Betenbaugh
authors.affiliation: Department of Pathology, Johns Hopkins University, Baltimore, MD, USA.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Institute for Bioscience and Biotechnology Research, University of Maryland College Park, Rockville, MD, USA.
authors.forename: Shuwei
authors.initials: S
authors.lastname: Li
mesh_terms: D000465:Algorithms; D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D006031:Glycosylation; D013058:Mass Spectrometry / Q000379:methods; D055432:Metabolomics / Q000379:methods*; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D015203:Reproducibility of Results
chemical_list: D011134:Polysaccharides