title: Inhibition of Glioblastoma Cell Growth In Vitro and In Vivo by Brucine, a Component of Chinese Medicine.
abstract: Glioblastoma multiforme (GBM) is one of the most common glial cell tumors and has drawn more and more attention in the clinic in recent years. Brucine has been reported to significantly suppress gastric cancer, lung cancer, and prostate cancer growth in vivo by inducing cell apoptosis. Here, the effects of brucine on U251 human glioma cell growth were investigated in vitro by cell proliferation assay, FACs, and qPCR in a xenograft tumor model. Treatment with brucine reduced the expression of BCL-2 and cyclooxygenase-2 (COX-2), while upregulated BAX expression in U251 human glioma cells resulted in reduced glioma cell survival rate and inhibited the growth of xenograft tumors. We concluded that brucine has a suppressive effect on U251 human glioma cells in vitro and in vivo, which could help in understanding the role of brucine in glioma cells and guiding drug use in the clinic.
authors.affiliation: Department of Pathology, Norman Bethune School of Medicine, Jilin University, Changchun, P. R. China.
authors.forename: Wang
authors.initials: W
authors.lastname: Ruijun
authors.forename: Meng
authors.initials: M
authors.lastname: Wenbin
authors.forename: Wang
authors.initials: W
authors.lastname: Yumin
authors.forename: Zhang
authors.initials: Z
authors.lastname: Ruijian
authors.forename: Huang
authors.initials: H
authors.lastname: Puweizhong
authors.forename: Li
authors.initials: L
authors.lastname: Yulin
mesh_terms: D000818:Animals; D001932:Brain Neoplasms / Q000188:drug therapy* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005260:Female; D005909:Glioblastoma / Q000188:drug therapy* / Q000473:pathology; D006131:Growth Inhibitors / Q000494:pharmacology / Q000627:therapeutic use*; D006801:Humans; D008516:Medicine, Chinese Traditional / Q000379:methods; D051379:Mice; D008819:Mice, Nude; D013331:Strychnine / Q000031:analogs & derivatives* / Q000627:therapeutic use; D023041:Xenograft Model Antitumor Assays / Q000379:methods
chemical_list: D006131:Growth Inhibitors; C083806:brucine; D013331:Strychnine