title: Functional analysis of UMOD gene and its effect on inflammatory cytokines in serum of essential hypertension patients.
abstract: OBJECTIVE
  The study aimed to investigate the function of uromodulin (UMOD) gene and its effect on inflammatory cytokines in serum of essential hypertension patients.
  
  
  METHODS
  The online database and software of computer were used for bioinformatics analysis on UMOD gene as well as the structure and function of its encoding proteins. Moreover, radioimmunoassay and enzyme linked immunosorbent assay was adopted to validate the content of urine UMOD protein of essential hypertension patients and their serum inflammatory cytokines.
  
  
  RESULTS
  As an alkaline and hydrophilic protein, UMOD has no transmembrane region, but it does have a signal peptide sequence. It is mainly located extracellularly, belonging to a secreted protein, whose secondary structure was based mainly on Random coil which account for 58.44%. According to function prediction, it is found that the UMOD protein has stress response which may be participate in the inflammatory reaction. It has been observed from the experiment which was designed on the basis of the correlation between inflammation reaction and essential hypertension that the content of urine UMOD protein of essential hypertension patients who is in stage I was (28.71 ± 10.53) mg/24 h and when compared with the control group's content (30.15 ± 14.10 mg/24 h), the difference was not obviously; The content of urine UMOD protein of essential hypertension patients who's in stage II and III was (18.24 ± 6.12) mg/24 h and (9.43 ± 3.16) mg/24 h, respectively, which were obviously lower than that of the control group (P<0.01). Additionally, the serum inflammatory cytokines, such as TNF-α, IL-6 and IL1-α content of essential hypertension patients were all markedly higher than that of control group (P<0.05).
  
  
  CONCLUSIONS
  For essential hypertension patients, there's a close relationship between the expression level of UMOD gene and inflammatory cytokines, which were manifested as the negative correlation between the level of the gene's expression and inflammatory cytokines. That has certain reference value to realize the targeted treatment for essential hypertension through regulated blood pressure conversely in the view of expression level of inflammatory cytokines.
authors.affiliation: Department of Cardiology, The Second Affiliated Hospital of Zhengzhou University Zhengzhou, Henan Province, China.
authors.forename: Liguo
authors.initials: L
authors.lastname: Jian
authors.affiliation: Department of Cardiology, The Second Affiliated Hospital of Zhengzhou University Zhengzhou, Henan Province, China.
authors.forename: Xian'en
authors.initials: X
authors.lastname: Fa
authors.affiliation: Department of Respiratory Medicine, The Second Affiliated Hospital of Zhengzhou University Zhengzhou, Henan Province, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: Department of Cardiology, The Second Affiliated Hospital of Zhengzhou University Zhengzhou, Henan Province, China.
authors.forename: Shichao
authors.initials: S
authors.lastname: Liu
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000097:blood / Q000652:urine; D001794:Blood Pressure; D016022:Case-Control Studies; D019295:Computational Biology; D016207:Cytokines / Q000097:blood*; D030541:Databases, Genetic; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D006973:Hypertension / Q000097:blood* / Q000175:diagnosis / Q000235:genetics / Q000503:physiopathology / Q000652:urine*; D018836:Inflammation Mediators / Q000097:blood*; D008297:Male; D008875:Middle Aged; D011237:Predictive Value of Tests; D060066:Protein Interaction Maps; D017433:Protein Structure, Secondary; D011863:Radioimmunoassay; D012720:Severity of Illness Index; D013329:Structure-Activity Relationship; D058949:Uromodulin / Q000737:chemistry / Q000235:genetics / Q000652:urine*
chemical_list: D015415:Biomarkers; D016207:Cytokines; D018836:Inflammation Mediators; C548612:UMOD protein, human; D058949:Uromodulin
keywords: UMOD; essential hypertension; gene ontology; inflammatory cytokines; protein function analysis