title: Sphere-Like Protein-Glycopolymer Nanostructures Tailored by Polyassociation.
abstract: Key parameters allow a reproducible polyassociation between avidin and biotinylated glycopolymers in order to fabricate defined supramolecular nanostructures for future (bio)medical and biotechnological applications. Thus, the polymerization efficiency of biotinylated glycopolymers in the fabrication of biohybrid structures (BHS) was investigated with regard to the influence of (i) the degree of biotinylation of the dendritic glycoarchitectures, (ii) two biotin linkers, (iii) the dendritic scaffold (perfectly branched vs hyperbranched), and (iv) the ligand-receptor stoichiometry. The adjustment of all these parameters opens the way to fabricate defined sizes of the final biohybrid structures as a multifunctional platform ready for their use in different applications. Various analytical techniques, including purification of BHS, were used to gain fundamental insights into the structural properties of the resulting protein-glycopolymer BHS. Finally, the elucidation of pivotal conformational properties of isolated BHS with defined sizes by asymmetrical flow field flow fractionation study revealed that they mainly possess spherical-/star-like properties. From this study, the fundamental knowledge can be likely transferred to other assemblies formed by molecular recognition processes (e.g., adamantane-β-cyclodextrin).
authors.affiliation: Leibniz-Institut für Polymerforschunng Dresden e.V. , Hohe Straße 6, D-01069 Dresden, Germany.|Technische Universität Dresden , 01062 Dresden, Germany.
authors.forename: Franka
authors.initials: F
authors.lastname: Ennen
authors.affiliation: Leibniz-Institut für Polymerforschunng Dresden e.V. , Hohe Straße 6, D-01069 Dresden, Germany.|Technische Universität Dresden , 01062 Dresden, Germany.
authors.forename: Philipp
authors.initials: P
authors.lastname: Fenner
authors.affiliation: Leibniz-Institut für Polymerforschunng Dresden e.V. , Hohe Straße 6, D-01069 Dresden, Germany.
authors.forename: Susanne
authors.initials: S
authors.lastname: Boye
authors.affiliation: Leibniz-Institut für Polymerforschunng Dresden e.V. , Hohe Straße 6, D-01069 Dresden, Germany.|Technische Universität Dresden , 01062 Dresden, Germany.
authors.forename: Albena
authors.initials: A
authors.lastname: Lederer
authors.affiliation: Leibniz-Institut für Polymerforschunng Dresden e.V. , Hohe Straße 6, D-01069 Dresden, Germany.
authors.forename: Hartmut
authors.initials: H
authors.lastname: Komber
authors.affiliation: Leibniz-Institut für Polymerforschunng Dresden e.V. , Hohe Straße 6, D-01069 Dresden, Germany.|Technische Universität Dresden , 01062 Dresden, Germany.
authors.forename: Brigitte
authors.initials: B
authors.lastname: Voit
authors.affiliation: Leibniz-Institut für Polymerforschunng Dresden e.V. , Hohe Straße 6, D-01069 Dresden, Germany.
authors.forename: Dietmar
authors.initials: D
authors.lastname: Appelhans
mesh_terms: D001360:Avidin / Q000737:chemistry; D001710:Biotin / Q000737:chemistry; D019921:Biotinylation / Q000379:methods; D031521:Fractionation, Field Flow / Q000379:methods; D008024:Ligands; D049329:Nanostructures / Q000737:chemistry*; D058105:Polymerization; D011108:Polymers / Q000737:chemistry*; D011485:Protein Binding; D013499:Surface Properties; D047392:beta-Cyclodextrins / Q000737:chemistry
chemical_list: D008024:Ligands; D011108:Polymers; D047392:beta-Cyclodextrins; D001360:Avidin; D001710:Biotin