title: Meibomian Gland Absence Related Dry Eye in Ectodysplasin A Mutant Mice.
abstract: Meibomian gland dysfunction is the most frequent cause of evaporative dry eye, yet its underlying pathophysiology is unknown. To gain insight into this pathophysiology, we characterized the time-dependent tear film and ocular surface changes occurring in X-linked anhidrotic-hypohidrotic ectodermal dysplasia mice (Tabby), which lack the meibomian gland. These mice sequentially developed corneal epithelial defects, central corneal stromal edema, neovascularization, and pannus 8 to 16 weeks after birth. Aqueous tear secretion was normal, whereas tear break-up time and ex vivo tear evaporation times were all shortened. Corneal epithelial microvilli were less numerous, conjunctival goblet cell density was unaffected, and MUC5AC and MUC5B gene expression was increased. Markers of squamous metaplasia (cytokeratin 10 and small proline-rich protein 1B) were noticed in the corneal epithelium of Tabby mice as early as the fourth week. Taken together, the Tabby mouse is a relevant meibomian gland dysfunction-related dry eye model that may lead to a better understanding of how meibomian glands are related to ocular surface health. This model may also help with discovering novel drug options for treating evaporative dry eye.
authors.affiliation: Eye Institute of Xiamen University, Xiamen, Fujian, China; Department of Ophthalmology & Visual Science, Medical College of Xiamen University, Xiamen, Fujian, China; Fujian Provincial Key Laboratory of Ophthalmology and Visual Science, Xiamen, Fujian, China.
authors.forename: Yen-Chiao
authors.initials: YC
authors.lastname: Wang
authors.affiliation: Eye Institute of Xiamen University, Xiamen, Fujian, China; Department of Ophthalmology & Visual Science, Medical College of Xiamen University, Xiamen, Fujian, China; Fujian Provincial Key Laboratory of Ophthalmology and Visual Science, Xiamen, Fujian, China.
authors.forename: Sanming
authors.initials: S
authors.lastname: Li
authors.affiliation: Eye Institute of Xiamen University, Xiamen, Fujian, China; Department of Ophthalmology & Visual Science, Medical College of Xiamen University, Xiamen, Fujian, China; Fujian Provincial Key Laboratory of Ophthalmology and Visual Science, Xiamen, Fujian, China.
authors.forename: Xiaoxiao
authors.initials: X
authors.lastname: Chen
authors.affiliation: Eye Institute of Xiamen University, Xiamen, Fujian, China; Department of Ophthalmology & Visual Science, Medical College of Xiamen University, Xiamen, Fujian, China; Fujian Provincial Key Laboratory of Ophthalmology and Visual Science, Xiamen, Fujian, China.
authors.forename: Baikai
authors.initials: B
authors.lastname: Ma
authors.affiliation: Eye Institute of Xiamen University, Xiamen, Fujian, China; Department of Ophthalmology & Visual Science, Medical College of Xiamen University, Xiamen, Fujian, China; Fujian Provincial Key Laboratory of Ophthalmology and Visual Science, Xiamen, Fujian, China.
authors.forename: Hui
authors.initials: H
authors.lastname: He
authors.affiliation: Eye Institute of Xiamen University, Xiamen, Fujian, China; Department of Ophthalmology & Visual Science, Medical College of Xiamen University, Xiamen, Fujian, China; Fujian Provincial Key Laboratory of Ophthalmology and Visual Science, Xiamen, Fujian, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Liu
authors.affiliation: Eye Institute of Xiamen University, Xiamen, Fujian, China; Department of Ophthalmology & Visual Science, Medical College of Xiamen University, Xiamen, Fujian, China; Fujian Provincial Key Laboratory of Ophthalmology and Visual Science, Xiamen, Fujian, China.
authors.forename: Jingwen
authors.initials: J
authors.lastname: Yu
authors.affiliation: Eye Institute of Xiamen University, Xiamen, Fujian, China; Department of Ophthalmology & Visual Science, Medical College of Xiamen University, Xiamen, Fujian, China; Fujian Provincial Key Laboratory of Ophthalmology and Visual Science, Xiamen, Fujian, China.
authors.forename: Liying
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Eye Institute of Xiamen University, Xiamen, Fujian, China; Department of Ophthalmology & Visual Science, Medical College of Xiamen University, Xiamen, Fujian, China; Fujian Provincial Key Laboratory of Ophthalmology and Visual Science, Xiamen, Fujian, China.
authors.forename: Yongxiong
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Eye Institute of Xiamen University, Xiamen, Fujian, China; Department of Ophthalmology & Visual Science, Medical College of Xiamen University, Xiamen, Fujian, China; Fujian Provincial Key Laboratory of Ophthalmology and Visual Science, Xiamen, Fujian, China; Department of Corneal & Ocular Surface Diseases, Xiamen University Affiliated Xiamen Eye Center, Xiamen, Fujian, China. Electronic address: zuguoliu@xmu.edu.cn.
authors.forename: Zuguo
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Eye Institute of Xiamen University, Xiamen, Fujian, China; Department of Ophthalmology & Visual Science, Medical College of Xiamen University, Xiamen, Fujian, China; Fujian Provincial Key Laboratory of Ophthalmology and Visual Science, Xiamen, Fujian, China; Department of Corneal & Ocular Surface Diseases, Xiamen University Affiliated Xiamen Eye Center, Xiamen, Fujian, China. Electronic address: wei1018@xmu.edu.cn.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D015352:Dry Eye Syndromes / Q000235:genetics / Q000473:pathology*; D053331:Ectodysplasins / Q000235:genetics; D005141:Eyelid Diseases / Q000235:genetics / Q000473:pathology*; D005455:Fluorescent Antibody Technique; D008297:Male; D008537:Meibomian Glands / Q000002:abnormalities*; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D008855:Microscopy, Electron, Scanning; D060888:Real-Time Polymerase Chain Reaction; D059467:Transcriptome
chemical_list: D053331:Ectodysplasins; C506019:Eda protein, mouse