title: Protective Effects of Manassantin A against Ethanol-Induced Gastric Injury in Rats.
abstract: Manassantin A, a neolignan isolated from Saururus chinensis, is a major phytochemical compound that has various biological activities, including anti-inflammatory, neuroleptic, and human acyl-CoA : cholesterol acyltransferase (ACAT) inhibitory activities. In this study, we investigated the protective effects of manassantin A against ethanol-induced acute gastric injury in rats. Gastric injury was induced by intragastric administration of 5 mL/kg body weight of absolute ethanol to each rat. The positive control group and the manassantin A group were given oral doses of omeprazole (20 mg/kg) or manassantin A (15 mg/kg), respectively, 1 h prior to the administration of absolute ethanol. Our examinations revealed that manassantin A pretreatment reduced ethanol-induced hemorrhage, hyperemia, and epithelial cell loss in the gastric mucosa. Manassantin A pretreatment also attenuated the increased lipid peroxidation associated with ethanol-induced acute gastric lesions, increased the mucosal glutathione (GSH) content, and enhanced the activities of antioxidant enzymes. The levels of pro-inflammatory cytokines, tumor necrosis factor-α (TNF-α), interleukin (IL)-6, and IL-1β were clearly decreased in the manassantin A-pretreated group. In addition, manassantin A pretreatment enhanced the levels of cyclooxygenase (COX)-1, COX-2, and prostaglandin E2 (PGE2) and reduced the inducible nitric oxide synthase (iNOS) overproduction and nuclear factor kappa B (NF-κB) phosphorylation. Collectively, these results indicate that manassantin A protects the gastric mucosa from ethanol-induced acute gastric injury, and suggest that these protective effects might be associated with COX/PGE2 stimulation, inhibition of iNOS production and NF-κB activation, and improvements in the antioxidant and anti-inflammatory status.
authors.affiliation: Department of Veterinary Pathology, College of Veterinary Medicine, Chungnam National University.
authors.forename: Ji-Won
authors.initials: JW
authors.lastname: Song
authors.forename: Chang-Seob
authors.initials: CS
authors.lastname: Seo
authors.forename: Tae-In
authors.initials: TI
authors.lastname: Kim
authors.forename: Og-Sung
authors.initials: OS
authors.lastname: Moon
authors.forename: Young-Suk
authors.initials: YS
authors.lastname: Won
authors.forename: Hwa-Young
authors.initials: HY
authors.lastname: Son
authors.forename: Jong-Keun
authors.initials: JK
authors.lastname: Son
authors.forename: Hyo-Jung
authors.initials: HJ
authors.lastname: Kwon
mesh_terms: D000818:Animals; D000897:Anti-Ulcer Agents / Q000737:chemistry / Q000494:pharmacology*; D002374:Catalase; D000431:Ethanol; D005978:Glutathione; D017705:Lignans / Q000737:chemistry / Q000494:pharmacology*; D008297:Male; D008315:Malondialdehyde; D015394:Molecular Structure; D009853:Omeprazole / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D032134:Saururaceae / Q000737:chemistry; D013272:Stomach Diseases / Q000139:chemically induced* / Q000517:prevention & control; D013482:Superoxide Dismutase
chemical_list: D000897:Anti-Ulcer Agents; D017705:Lignans; D000431:Ethanol; D008315:Malondialdehyde; C054895:manassantin A; D002374:Catalase; D013482:Superoxide Dismutase; D005978:Glutathione; D009853:Omeprazole