title: Activation of NLRP3 inflammasome in human neutrophils by Helicobacter pylori infection.
abstract: TLRs and NLRs participate in the immune system recognition of Helicobacter pylori. However, little is known about the mechanisms leading to inflammasome activation by H. pylori and if NLRs in neutrophils are involved in the process. We studied how NOD-like receptor family, pyrin domain-containing 3 (NLRP3) inflammasome components are involved in IL-1β maturation in human neutrophils in response to the infection and if they are dependent on T4SS (type IV secretion system) and TLRs. Human neutrophils were cultured and infected with the 26695 or the VirD4- H. pylori strains; the IL-1β concentration was analyzed by ELISA, and we also evaluated the activation of TLRs 2 and 4. The infection of neutrophils with both strains of H. pylori induced production of IL-1β and expression of the NLRP3 inflammasome components such as apoptosis-associated speck-like protein with CARD domain and NLRP3 protein. The infection also increased the activity of caspase-1, which is required for the maturation of IL-1β. Our study shows, for the first time, that H. pylori infection induces the expression and activation of components of NLRP3 inflammasomes in human neutrophils and that the activation is independent of a functional T4SS and TLR2 and TLR4.
authors.affiliation: Laboratorio de Investigación en Inmunología y Proteómica, Hospital Infantil de México Federico Gómez, México City, México Unidad de Investigación Médica en Enfermedades Infecciosas, Hospital de Pediatría, CMN SXXI, IMSS, México City, México.
authors.forename: Erandi
authors.initials: E
authors.lastname: Pérez-Figueroa
authors.affiliation: Unidad de Investigación Médica en Enfermedades Infecciosas, Hospital de Pediatría, CMN SXXI, IMSS, México City, México.
authors.forename: Javier
authors.initials: J
authors.lastname: Torres
authors.affiliation: Laboratorio de Investigación en Inmunología y Proteómica, Hospital Infantil de México Federico Gómez, México City, México Unidad de Investigación Médica en Enfermedades Infecciosas, Hospital de Pediatría, CMN SXXI, IMSS, México City, México.
authors.forename: Norma
authors.initials: N
authors.lastname: Sánchez-Zauco
authors.affiliation: Laboratorio de Biología del Desarrollo, Hospital Infantil de México Federico Gómez, México City, México.
authors.forename: Alejandra
authors.initials: A
authors.lastname: Contreras-Ramos
authors.affiliation: CONACYT Research Fellow-Hospital Infantil de México Federico Gómez.
authors.forename: Lourdes
authors.initials: L
authors.lastname: Alvarez-Arellano
authors.affiliation: Laboratorio de Investigación en Inmunología y Proteómica, Hospital Infantil de México Federico Gómez, México City, México cmaldobe@yahoo.com.
authors.forename: Carmen
authors.initials: C
authors.lastname: Maldonado-Bernal
mesh_terms: D017209:Apoptosis; D002352:Carrier Proteins / Q000378:metabolism*; D020170:Caspase 1 / Q000378:metabolism; D002478:Cells, Cultured; D016481:Helicobacter Infections / Q000276:immunology*; D016480:Helicobacter pylori / Q000276:immunology*; D006801:Humans; D058847:Inflammasomes / Q000378:metabolism*; D053583:Interleukin-1beta / Q000378:metabolism; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; D009504:Neutrophils / Q000276:immunology* / Q000382:microbiology; D015398:Signal Transduction; D051195:Toll-Like Receptor 2 / Q000378:metabolism; D051197:Toll-Like Receptor 4 / Q000378:metabolism
chemical_list: D002352:Carrier Proteins; D058847:Inflammasomes; D053583:Interleukin-1beta; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; C453881:NLRP3 protein, human; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4; D020170:Caspase 1
keywords: Helicobacter pylori; IL-1β; Inflammasome; human neutrophils; inflammatory response