title: Integrated micro-biochemical approach for phytoremediation of cadmium and lead contaminated soils using Gladiolus grandiflorus L cut flower.
abstract: The potential of vermicompost, elemental sulphur, Thiobacillus thiooxidans and Pseudomonas putida for phytoremediation is well known individually but their integrated approach has not been discovered so far. The present work highlights the consideration of so far overlooked aspects of their integrated treatment by growing the ornamental plant, Gladiolus grandiflorus L in uncontaminated and sewage-contaminated soils (sulphur-deficient alluvial Entisols, pH 7.6-7.8) for phytoremediation of cadmium and lead under pot experiment. Between vermicompost and elemental sulphur, the response of vermicompost was higher towards improvement in the biometric parameters of plants, whereas the response of elemental sulphur was higher towards enhanced bioaccumulation of heavy metals under soils. The integrated treatment (T7: vermicompost 6g and elemental sulphur 0.5gkg(-1) soil and co-inoculation of the plant with T. thiooxidans and P. putida) was found superior in promoting root length, plant height and dry biomass of the plant. The treatment T7 caused enhanced accumulation of Cd up to 6.96 and 6.45mgkg(-1) and Pb up to 22.6 and 19.9mgkg(-1) in corm and shoot, respectively at the contaminated soil. T7 showed maximum remediation efficiency of 0.46% and 0.19% and bioaccumulation factor of 2.92 and 1.21 and uptake of 6.75 and 21.4mgkg(-1) dry biomass for Cd and Pb respectively in the contaminated soil. The integrated treatment T7 was found significant over the individual treatments to promote plant growth and enhance phytoremediation. Hence, authors conclude to integrate vermicompost, elemental sulphur and microbial co-inoculation for the enhanced clean-up of Cd and Pb-contaminated soils.
authors.affiliation: Sheila Dhar Institute of Soil Science, Department of Chemistry, University of Allahabad, Allahabad 211002, India.
authors.forename: Dinesh
authors.initials: D
authors.lastname: Mani
authors.affiliation: The National Academy of Sciences, India, Allahabad 211002, India. Electronic address: chitranjan.alld@gmail.com.
authors.forename: Chitranjan
authors.initials: C
authors.lastname: Kumar
authors.affiliation: Sheila Dhar Institute of Soil Science, Department of Chemistry, University of Allahabad, Allahabad 211002, India.
authors.forename: Niraj Kumar
authors.initials: NK
authors.lastname: Patel
mesh_terms: D013856:Acidithiobacillus thiooxidans; D001673:Biodegradation, Environmental; D018533:Biomass; D002104:Cadmium / Q000032:analysis / Q000378:metabolism; D035264:Flowers; D027621:Iridaceae / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D007854:Lead / Q000032:analysis / Q000378:metabolism; D063245:Plant Development / Q000187:drug effects; D016958:Pseudomonas putida; D012987:Soil / Q000737:chemistry; D012989:Soil Pollutants / Q000032:analysis / Q000378:metabolism*; D013455:Sulfur / Q000494:pharmacology*
chemical_list: D012987:Soil; D012989:Soil Pollutants; D002104:Cadmium; D007854:Lead; D013455:Sulfur
keywords: Elemental sulphur; Gladiolus grandiflorus L; Heavy metals; Microbe-assisted phytoremediation; Uptake; Vermicompost