title: Evidence of 18F-FCH Uptake in Human T98G Glioblastoma Cells.
abstract: OBJECTIVE
  Tumor and chemo/radiotherapy-damaged brain tissues are hardly distinguishable by conventional morphological imaging. (18)F-FCH was compared against (18)F-FDG in the T98G glioblastoma cell line with regard to their radiopharmaceutical uptake, in order to test its diagnostic power on brain tumor lesions.
  
  
  METHODS
  Equimolar amounts of (18)F-FCH and (18)F-FDG were added to human glioblastoma T98G cells and human dermal fibroblasts for 20, 40, 60, 90 and 120 min of incubation. Radiopharmaceutical uptake was expressed as a percentage of the administered dose. Cold choline was used for binding competition experiments.
  
  
  RESULTS
  In T98G cells (18)F-FCH was taken-up in higher amounts than 18F-FDG after 60 min. In fibroblasts, uptake was lower than 1% for both radiopharmaceuticals. Cold choline reduced the uptake of FCH to 1% similarly to fibroblasts.
  
  
  CONCLUSIONS
  Our results prove the efficacy of (18)F-FCH as a promising tracer, better than (18)F-FDG in establishing the tumor-to-background ratio in brain tumors.
authors.affiliation: Department of Oncohaematology, Nuclear Medicine Unit, IRCCS San Matteo Hospital Foundation, Pavia, Italy.
authors.forename: Federica Eleonora
authors.initials: FE
authors.lastname: Buroni
authors.affiliation: Department of Oncohaematology, Radiotherapy Unit, IRCCS San Matteo Hospital Foundation, Pavia, Italy Department of Biology and Biotechnology "Lazzaro Spallanzani", University of Pavia, Pavia, Italy francesca.pasi@gmail.com.
authors.forename: Francesca
authors.initials: F
authors.lastname: Pasi
authors.affiliation: Department of Oncohaematology, Nuclear Medicine Unit, IRCCS San Matteo Hospital Foundation, Pavia, Italy.
authors.forename: Marco Giovanni
authors.initials: MG
authors.lastname: Persico
authors.affiliation: Department of Oncohaematology, Nuclear Medicine Unit, IRCCS San Matteo Hospital Foundation, Pavia, Italy.
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Lodola
authors.affiliation: Department of Oncohaematology, Nuclear Medicine Unit, IRCCS San Matteo Hospital Foundation, Pavia, Italy.
authors.forename: Carlo
authors.initials: C
authors.lastname: Aprile
authors.affiliation: Department of Biology and Biotechnology "Lazzaro Spallanzani", University of Pavia, Pavia, Italy.
authors.forename: Rosanna
authors.initials: R
authors.lastname: Nano
mesh_terms: D001932:Brain Neoplasms / Q000000981:diagnostic imaging* / Q000378:metabolism*; D045744:Cell Line, Tumor; D002794:Choline / Q000031:analogs & derivatives* / Q000493:pharmacokinetics; D019788:Fluorodeoxyglucose F18 / Q000493:pharmacokinetics*; D005909:Glioblastoma / Q000000981:diagnostic imaging* / Q000378:metabolism*; D006801:Humans; D011877:Radionuclide Imaging; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics*
chemical_list: D019275:Radiopharmaceuticals; D019788:Fluorodeoxyglucose F18; C514960:fluorocholine; D002794:Choline
keywords: 18F-fluorocholine; 18F-fluorodeoxyglucose; Glioma; T98G human cells; radiation injury