title: Ungulate copiparvovirus 1 (bovine parvovirus 2): characterization of a new genotype and associated viremia in different bovine age groups.
abstract: A novel bovine parvovirus 2 (BPV2) genotype comprising 5394 nt was identified by next generation sequencing from sera of healthy cattle at different age groups farmed in the state of Rio Grande do Sul, Brazil. The genome organization of new BPV2 genotype retains the two ORFs typical of members of the Parvovirinae with 86.4 % of overall nucleotide sequence identities in comparison to other members of the subfamily. Phylogenetic analysis revealed similar clustering with two previously described bovine BPV2 within the genus Copiparvovirus. No significant differences (P ≥ 0.05) were detected in the distribution of BPV2 infection in cattle at different age groups. This is the third complete or near complete genome sequence of BPV2 reported to date and may contribute to a better understanding of the biology of copiparvoviruses and its interactions with the host.
authors.affiliation: Virology, FEPAGRO Animal Health - Instituto de Pesquisas Veterinárias Desidério Finamor (IPVDF), Estrada do Conde 6000, Eldorado do Sul, RS, 92990-000, Brazil. spcibulski@gmail.com.|Virology Laboratory, Department of Microbiology, Immunology and Parasitology, Instituto de Ciências Básicas da Saúde, UFRGS. Av. Sarmento Leite 500, sala 208, Porto Alegre, RS, 90050-170, Brazil. spcibulski@gmail.com.
authors.forename: Samuel Paulo
authors.initials: SP
authors.lastname: Cibulski
authors.affiliation: Virology, FEPAGRO Animal Health - Instituto de Pesquisas Veterinárias Desidério Finamor (IPVDF), Estrada do Conde 6000, Eldorado do Sul, RS, 92990-000, Brazil.|Virology Laboratory, Department of Microbiology, Immunology and Parasitology, Instituto de Ciências Básicas da Saúde, UFRGS. Av. Sarmento Leite 500, sala 208, Porto Alegre, RS, 90050-170, Brazil.
authors.forename: Thais Fumaco
authors.initials: TF
authors.lastname: Teixeira
authors.affiliation: Virology, FEPAGRO Animal Health - Instituto de Pesquisas Veterinárias Desidério Finamor (IPVDF), Estrada do Conde 6000, Eldorado do Sul, RS, 92990-000, Brazil.
authors.forename: Helton Fernandes
authors.initials: HF
authors.lastname: Dos Santos
authors.affiliation: Virology Laboratory, Department of Microbiology, Immunology and Parasitology, Instituto de Ciências Básicas da Saúde, UFRGS. Av. Sarmento Leite 500, sala 208, Porto Alegre, RS, 90050-170, Brazil.
authors.forename: Francisco Esmaile
authors.initials: FE
authors.lastname: de Sales Lima
authors.affiliation: Virology, FEPAGRO Animal Health - Instituto de Pesquisas Veterinárias Desidério Finamor (IPVDF), Estrada do Conde 6000, Eldorado do Sul, RS, 92990-000, Brazil.
authors.forename: Camila Mengue
authors.initials: CM
authors.lastname: Scheffer
authors.affiliation: Virology, FEPAGRO Animal Health - Instituto de Pesquisas Veterinárias Desidério Finamor (IPVDF), Estrada do Conde 6000, Eldorado do Sul, RS, 92990-000, Brazil.
authors.forename: Ana Paula Muterle
authors.initials: AP
authors.lastname: Varela
authors.affiliation: Virology, FEPAGRO Animal Health - Instituto de Pesquisas Veterinárias Desidério Finamor (IPVDF), Estrada do Conde 6000, Eldorado do Sul, RS, 92990-000, Brazil.
authors.forename: Diane Alves
authors.initials: DA
authors.lastname: de Lima
authors.affiliation: Virology, FEPAGRO Animal Health - Instituto de Pesquisas Veterinárias Desidério Finamor (IPVDF), Estrada do Conde 6000, Eldorado do Sul, RS, 92990-000, Brazil.
authors.forename: Candice
authors.initials: C
authors.lastname: Schmidt
authors.affiliation: Departamento de Desarrollo Biotecnológico, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay.
authors.forename: Fernando
authors.initials: F
authors.lastname: Silveira
authors.affiliation: Virology, FEPAGRO Animal Health - Instituto de Pesquisas Veterinárias Desidério Finamor (IPVDF), Estrada do Conde 6000, Eldorado do Sul, RS, 92990-000, Brazil.
authors.forename: Laura Lopes
authors.initials: LL
authors.lastname: de Almeida
authors.affiliation: Virology, FEPAGRO Animal Health - Instituto de Pesquisas Veterinárias Desidério Finamor (IPVDF), Estrada do Conde 6000, Eldorado do Sul, RS, 92990-000, Brazil.|Virology Laboratory, Department of Microbiology, Immunology and Parasitology, Instituto de Ciências Básicas da Saúde, UFRGS. Av. Sarmento Leite 500, sala 208, Porto Alegre, RS, 90050-170, Brazil.
authors.forename: Paulo Michel
authors.initials: PM
authors.lastname: Roehe
mesh_terms: D000367:Age Factors; D000818:Animals; D052660:Bocavirus / Q000145:classification / Q000235:genetics*; D001938:Brazil; D002417:Cattle / Q000821:virology*; D004279:DNA, Viral; D016679:Genome, Viral; D005838:Genotype; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D014766:Viremia / Q000662:veterinary
chemical_list: D004279:DNA, Viral
keywords: Bovine parvovirus 2; Copiparvovirus; High-throughput sequencing; NGS; Sera samples