title: Dengue virus infection-enhancing antibody activities against Indonesian strains in inhabitants of central Thailand.
abstract: Dengue virus (DENV) infection-enhancing antibodies are a hypothetic factor to increase the dengue disease severity. In this study, we investigated the enhancing antibodies against Indonesian strains of DENV-1-4 in 50 healthy inhabitants of central Thailand (Bangkok and Uthai Thani). Indonesia and Thailand have seen the highest dengue incidence in Southeast Asia. The infection history of each subject was estimated by comparing his/her neutralizing antibody titers against prototype DENV-1-4 strains. To resolve the difficulty in obtaining foreign live viruses for use as assay antigens, we used a recombinant system to prepare single-round infectious dengue viral particles based on viral sequence information. Irrespective of the previously infecting serotype(s), most serum samples showed significantly higher enhancement titers against Indonesian DENV-2 strains than against Thai DENV-2 strains, whereas the opposite effect was observed for the DENV-3 strains. Equivalent enhancing activities were observed against both DENV-1 and DENV-4. These results suggest that the genotype has an impact on enhancing antibody activities against DENV-2 and DENV-3, because the predominant circulating genotypes of each serotype differ between Indonesia and Thailand.
authors.affiliation: BIKEN Endowed Department of Dengue Vaccine Development, Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, Thailand; BIKEN Endowed Department of Dengue Vaccine Development, Research Institute for Microbial Diseases, Osaka University, Osaka 565-0871, Japan. Electronic address: knmya@biken.osaka-u.ac.jp.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Yamanaka
authors.affiliation: BIKEN Endowed Department of Dengue Vaccine Development, Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, Thailand; BIKEN Endowed Department of Dengue Vaccine Development, Research Institute for Microbial Diseases, Osaka University, Osaka 565-0871, Japan.
authors.forename: Duangjai
authors.initials: D
authors.lastname: Oddgun
authors.affiliation: Mahidol-Osaka Center for Infectious Diseases, Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, Thailand; Mahidol-Osaka Center for Infectious Diseases, Research Institute for Microbial Diseases, Osaka University, Osaka 565-0871, Japan.
authors.forename: Nantarat
authors.initials: N
authors.lastname: Chantawat
authors.affiliation: Mahidol-Osaka Center for Infectious Diseases, Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, Thailand; Mahidol-Osaka Center for Infectious Diseases, Research Institute for Microbial Diseases, Osaka University, Osaka 565-0871, Japan.
authors.forename: Tamaki
authors.initials: T
authors.lastname: Okabayashi
authors.affiliation: Center of Excellence for Antibody Research, Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, Thailand. Electronic address: pongrama.ram@mahidol.ac.th.
authors.forename: Pongrama
authors.initials: P
authors.lastname: Ramasoota
authors.affiliation: Indonesia-Japan Collaborative Research Center for Emerging and Re-emerging Infectious Diseases, Institute of Tropical Disease, Airlangga University, Surabaya 60015, Indonesia.
authors.forename: Siti
authors.initials: S
authors.lastname: Churrotin
authors.affiliation: Indonesia-Japan Collaborative Research Center for Emerging and Re-emerging Infectious Diseases, Institute of Tropical Disease, Airlangga University, Surabaya 60015, Indonesia; Department of International Health, Kobe University Graduate School of Health Sciences, Hyogo 654-0142, Japan.
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Kotaki
authors.affiliation: Department of International Health, Kobe University Graduate School of Health Sciences, Hyogo 654-0142, Japan.
authors.forename: Masanori
authors.initials: M
authors.lastname: Kameoka
authors.affiliation: Indonesia-Japan Collaborative Research Center for Emerging and Re-emerging Infectious Diseases, Institute of Tropical Disease, Airlangga University, Surabaya 60015, Indonesia.
authors.forename: Soegeng
authors.initials: S
authors.lastname: Soegijanto
authors.affiliation: BIKEN Endowed Department of Dengue Vaccine Development, Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, Thailand; BIKEN Endowed Department of Dengue Vaccine Development, Research Institute for Microbial Diseases, Osaka University, Osaka 565-0871, Japan.
authors.forename: Eiji
authors.initials: E
authors.lastname: Konishi
mesh_terms: D000293:Adolescent; D000328:Adult; D019138:Antibodies, Blocking / Q000097:blood*; D057134:Antibodies, Neutralizing / Q000097:blood; D003716:Dengue Virus / Q000145:classification / Q000276:immunology* / Q000472:pathogenicity; D005260:Female; D064368:Healthy Volunteers; D006801:Humans; D008297:Male; D009500:Neutralization Tests; D016036:Seroepidemiologic Studies; D065288:Serogroup; D013785:Thailand / Q000453:epidemiology; D055815:Young Adult
chemical_list: D019138:Antibodies, Blocking; D057134:Antibodies, Neutralizing
keywords: Antibody-dependent enhancement; Dengue; Indonesia; Recombinant proteins; Thailand; Viral antigens