title: Bone Morphogenetic Protein-6 (BMP-6) Stimulates the Antrum Formation by the Regulation of its Signalling Pathway in Caprine Pre-antral Follicles Cultured In Vitro.
abstract: BMP-6 has been found to be important to ovarian cells and oocyte, as well as to uterus. Thus, this study investigated the effect of bone morphogenetic protein (BMP-6) and recombinant follicle-stimulating hormone (rFSH) alone or in combination on the in vitro culture (IVC) of isolated caprine secondary follicles (Experiment 1) and the mRNA levels for BMP receptors/Smad signalling pathway (BMPR1A, BMPR2, SMAD1, SMAD4, SMAD5, SMAD6, SMAD7 and SMAD8) in vivo and in vitro using BMP-6 (Experiment 2). Secondary follicles were cultured in αMEM(+) alone (control medium) or supplemented with BMP-6 at 1 or 10 ng/ml and rFSH alone or the combination of both BMP-6 concentrations and rFSH. The results from Experiment 1 showed that the antrum formation rate was higher in the BMP-6 at 1 ng/ml (p < 0.05) than in MEM. In Experiment 2, the mRNA expression for BMPR2, SMAD1, SMAD5 and SMAD6 was detected in non-cultured control and after in vitro culture (MEM and 1 ng/ml BMP-6); while the expression of SMAD7 and SMAD8 mRNA was only detected after IVC, SMAD4 was only detected in the BMP-6 at 1 ng/ml treatment. In conclusion, the low BMP-6 concentration positively influenced antrum formation and ensured normal mRNA expression for BMP receptor and Smads after IVC of caprine secondary follicles.
authors.affiliation: Faculty of Veterinary Medicine, Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), State University of Ceará, Fortaleza, CE, Brazil.
authors.forename: V R
authors.initials: VR
authors.lastname: Araújo
authors.affiliation: Faculty of Veterinary Medicine, Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), State University of Ceará, Fortaleza, CE, Brazil.
authors.forename: G M
authors.initials: GM
authors.lastname: Silva
authors.affiliation: Medical School, Morphology Department, Federal University of Ceará, Fortaleza, CE, Brazil.
authors.forename: A B G
authors.initials: AB
authors.lastname: Duarte
authors.affiliation: Faculty of Veterinary Medicine, Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), State University of Ceará, Fortaleza, CE, Brazil.
authors.forename: D M
authors.initials: DM
authors.lastname: Magalhães-Padilha
authors.affiliation: Laboratory of Molecular Biology and Development, University of Fortaleza, Fortaleza, CE, Brazil.
authors.forename: A P
authors.initials: AP
authors.lastname: Almeida
authors.affiliation: Faculty of Veterinary Medicine, Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), State University of Ceará, Fortaleza, CE, Brazil.
authors.forename: F O
authors.initials: FO
authors.lastname: Lunardi
authors.affiliation: Faculty of Veterinary Medicine, Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), State University of Ceará, Fortaleza, CE, Brazil.
authors.forename: M K B
authors.initials: MK
authors.lastname: Serafim
authors.affiliation: Laboratory of Animal Physiology, Department of Animal Science, Federal University of Ceará, Fortaleza, CE, Brazil.
authors.forename: A A A
authors.initials: AA
authors.lastname: Moura
authors.affiliation: Faculty of Veterinary Medicine, Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), State University of Ceará, Fortaleza, CE, Brazil.
authors.forename: C C
authors.initials: CC
authors.lastname: Campello
authors.affiliation: Faculty of Veterinary Medicine, Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), State University of Ceará, Fortaleza, CE, Brazil.
authors.forename: A P R
authors.initials: AP
authors.lastname: Rodrigues
authors.affiliation: Faculty of Veterinary Medicine, Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), State University of Ceará, Fortaleza, CE, Brazil.
authors.forename: J R
authors.initials: JR
authors.lastname: Figueiredo
mesh_terms: D000818:Animals; D055418:Bone Morphogenetic Protein 6 / Q000494:pharmacology*; D052004:Bone Morphogenetic Protein Receptors / Q000235:genetics; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D006041:Goats / Q000502:physiology*; D006080:Ovarian Follicle / Q000187:drug effects* / Q000502:physiology*; D012333:RNA, Messenger / Q000032:analysis; D011994:Recombinant Proteins / Q000494:pharmacology; D015398:Signal Transduction / Q000187:drug effects*; D046509:Tissue Culture Techniques / Q000662:veterinary
chemical_list: D055418:Bone Morphogenetic Protein 6; D012333:RNA, Messenger; D011994:Recombinant Proteins; D005640:Follicle Stimulating Hormone; D052004:Bone Morphogenetic Protein Receptors