title: Genetic diversity analysis of Capparis spinosa L. populations by using ISSR markers.
abstract: Capparis spinosa L. is an important medicinal species in the Xinjiang Province of China. Ten natural populations of C. spinosa from 3 locations in North, Central, and South Xinjiang were studied using morphological trait inter simple sequence repeat (ISSR) molecular markers to assess the genetic diversity and population structure. In this study, the 10 ISSR primers produced 313 amplified DNA fragments, with 52% of fragments being polymorphic. Unweighted pair-group method with arithmetic average (UPGMA) cluster analysis indicated that 10 C. spinosa populations were clustered into 3 geographically distinct groups. The Nei gene of C. spinosa populations in different regions had Diversity and Shannon's information index ranges of 0.1312-0.2001 and 0.1004-0.1875, respectively. The 362 markers were used to construct the dendrogram based on the UPGMA cluster analysis. The dendrogram indicated that 10 populations of C. spinosa were clustered into 3 geographically distinct groups. The results showed these genotypes have high genetic diversity, and can be used for an alternative breeding program.
authors.affiliation: Xinjiang Institute of Materia Medica, WLMQ, Xinjiang Province, China liu_chong02@163.com.
authors.forename: C
authors.initials: C
authors.lastname: Liu
authors.affiliation: Xinjiang Institute of Materia Medica, WLMQ, Xinjiang Province, China.
authors.forename: G P
authors.initials: GP
authors.lastname: Xue
authors.affiliation: Xinjiang Institute of Materia Medica, WLMQ, Xinjiang Province, China.
authors.forename: B
authors.initials: B
authors.lastname: Cheng
authors.affiliation: Xinjiang Institute of Materia Medica, WLMQ, Xinjiang Province, China.
authors.forename: X
authors.initials: X
authors.lastname: Wang
authors.affiliation: Xinjiang Institute of Materia Medica, WLMQ, Xinjiang Province, China.
authors.forename: J
authors.initials: J
authors.lastname: He
authors.affiliation: Xinjiang Institute of Materia Medica, WLMQ, Xinjiang Province, China.
authors.forename: G H
authors.initials: GH
authors.lastname: Liu
authors.affiliation: Xinjiang Institute of Materia Medica, WLMQ, Xinjiang Province, China.
authors.forename: W J
authors.initials: WJ
authors.lastname: Yang
mesh_terms: D031484:Capparis / Q000145:classification / Q000235:genetics*; D016000:Cluster Analysis; D005819:Genetic Markers; D014644:Genetic Variation; D005828:Genetics, Population; D005838:Genotype; D018895:Microsatellite Repeats; D058885:Multilocus Sequence Typing; D010802:Phylogeny; D011110:Polymorphism, Genetic; D019655:Quantitative Trait, Heritable
chemical_list: D005819:Genetic Markers