title: Genetic Determinants of RNA Editing Levels of ADAR Targets in Drosophila melanogaster.
abstract: RNA editing usually affects only a fraction of expressed transcripts and there is a vast amount of variation in editing levels of ADAR (adenosine deaminase, RNA-specific) targets. Here we explore natural genetic variation affecting editing levels of particular sites in 81 natural strains of Drosophila melanogaster. The analysis of associations between editing levels and single-nucleotide polymorphisms allows us to map putative cis-regulatory regions affecting editing of 16 A-to-I editing sites (cis-RNA editing quantitative trait loci or cis-edQTLs, P < 10(-8)). The observed changes in editing levels are validated by independent molecular technique. All identified regulatory variants are located in close proximity of modulated editing sites. Moreover, colocalized editing sites are often regulated by same loci. Similar to expression and splicing QTL studies, the characterization of edQTLs will greatly expand our understanding of cis-regulatory evolution of gene expression.
authors.affiliation: Department of Biological Sciences, University of Southern California, Los Angeles, California 90089 Institute for Information Transmission Problems, Moscow, Russia 127994 kurmanga@usc.edu snuzhdin@usc.edu.
authors.forename: Yerbol Z
authors.initials: YZ
authors.lastname: Kurmangaliyev
authors.affiliation: Department of Biological Sciences, University of Southern California, Los Angeles, California 90089.
authors.forename: Sammi
authors.initials: S
authors.lastname: Ali
authors.affiliation: Department of Biological Sciences, University of Southern California, Los Angeles, California 90089 Department of Computational Biology, Saint Petersburg Polytechnical University, Saint Petersburg, Russia 195251 kurmanga@usc.edu snuzhdin@usc.edu.
authors.forename: Sergey V
authors.initials: SV
authors.lastname: Nuzhdin
mesh_terms: D000243:Adenosine Deaminase / Q000235:genetics*; D000818:Animals; D002874:Chromosome Mapping; D029721:Drosophila Proteins / Q000235:genetics*; D004331:Drosophila melanogaster / Q000235:genetics*; D020869:Gene Expression Profiling; D014644:Genetic Variation; D040641:Quantitative Trait Loci; D017393:RNA Editing; D015203:Reproducibility of Results; D059467:Transcriptome
chemical_list: D029721:Drosophila Proteins; C567956:Adar protein, Drosophila; D000243:Adenosine Deaminase
keywords: Drosophila; RNA editing; natural variation; quantitative trait loci