title: Studying Cell Division Plane Positioning in Early-Stage Embryos.
abstract: Unraveling the mechanisms that govern division plane orientation is a major challenge to understand plant development. In this respect, the Arabidopsis early embryo is a model system of choice since embryogenesis is relatively simple and cell division planes orientation is highly predictable. Here, we present an integrated set of protocols to study 3D cell division patterns in early-stage Arabidopsis embryos that combine both cellular and sub-cellular localization of selected protein markers with spatial organization of cells, cytoskeleton, and nuclei.
authors.affiliation: Institut Jean-Pierre Bourgin (IJPB), INRA, UMR 1318, ERL 3559 CNRS, Saclay Plant Sciences, 78000, Versailles, France. Katia.Belcram@versailles.inra.fr.|Institut Jean-Pierre Bourgin (IJPB), AgroParisTech, UMR 1318, ERL 3559 CNRS, Saclay Plant Sciences, 78000, Versailles, France. Katia.Belcram@versailles.inra.fr.
authors.forename: Katia
authors.initials: K
authors.lastname: Belcram
authors.affiliation: Institut Jean-Pierre Bourgin (IJPB), INRA, UMR 1318, ERL 3559 CNRS, Saclay Plant Sciences, 78000, Versailles, France.|Institut Jean-Pierre Bourgin (IJPB), AgroParisTech, UMR 1318, ERL 3559 CNRS, Saclay Plant Sciences, 78000, Versailles, France.
authors.forename: Jean-Christophe
authors.initials: JC
authors.lastname: Palauqui
authors.affiliation: Institut Jean-Pierre Bourgin (IJPB), INRA, UMR 1318, ERL 3559 CNRS, Saclay Plant Sciences, 78000, Versailles, France.|Institut Jean-Pierre Bourgin (IJPB), AgroParisTech, UMR 1318, ERL 3559 CNRS, Saclay Plant Sciences, 78000, Versailles, France.
authors.forename: Martine
authors.initials: M
authors.lastname: Pastuglia
mesh_terms: D017360:Arabidopsis / Q000166:cytology* / Q000196:embryology*; D002455:Cell Division; D004210:Dissection / Q000379:methods; D005453:Fluorescence; D007150:Immunohistochemistry / Q000379:methods*; D018613:Microscopy, Confocal / Q000379:methods*; D008870:Microtubules / Q000648:ultrastructure; D012639:Seeds / Q000166:cytology* / Q000648:ultrastructure; D013194:Staining and Labeling / Q000379:methods; D016707:Tissue Fixation / Q000379:methods
keywords: Arabidopsis; Cell wall; Embryo; Fluorescent marker; Immunolocalization; Microtubules; Whole-mount