title: Juvenile zebrafish in the vitellogenin blank period as an alternative test organism for evaluation of estrogenic activity of chemicals.
abstract: The present study aimed to determine the suitable development period for zebrafish to evaluate estrogenic activities accurately. An enzyme-linked immunosorbent assay was developed and used to detect the vitellogenin (Vtg)-derived yolk proteins and newly produced Vtg, and 9 d to 56 d posthatching was determined as the Vtg-blank period. Juveniles in this period were found to have lower baseline Vtg levels than adult males and were considered an alternative test organism for detecting environmental estrogens. Environ Toxicol Chem 2016;35:1783-1787. © 2015 SETAC.
authors.affiliation: Marine Life Science College, Ocean University of China, Qingdao, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.affiliation: Marine Life Science College, Ocean University of China, Qingdao, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Zhao
authors.affiliation: Marine Life Science College, Ocean University of China, Qingdao, China.
authors.forename: Ruihou
authors.initials: R
authors.lastname: Shan
authors.affiliation: Marine Life Science College, Ocean University of China, Qingdao, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Tian
authors.affiliation: Marine Life Science College, Ocean University of China, Qingdao, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Marine Life Science College, Ocean University of China, Qingdao, China.
authors.forename: Shaoguo
authors.initials: S
authors.lastname: Ru
mesh_terms: D000818:Animals; D004784:Environmental Monitoring / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay; D004958:Estradiol / Q000633:toxicity*; D004967:Estrogens / Q000633:toxicity*; D005260:Female; D008297:Male; D014819:Vitellogenins / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D015027:Zebrafish / Q000254:growth & development* / Q000378:metabolism
chemical_list: D004967:Estrogens; D014819:Vitellogenins; D014874:Water Pollutants, Chemical; D004958:Estradiol
keywords: Biomarker; Environmental estrogens; Juvenile zebrafish; Vitellogenin