title: ANTIOXIDANT ACTIVITY AND FREE RADICAL SCAVENGING CAPACITY OF L-ARGININE AND NAHS: A COMPARATIVE IN VITRO STUDY.
abstract: In the family of gaseous transmitters, hydrogen sulfide (H2S) is considered as third member beside nitric oxide (NO) and carbon monoxide (CO), which can play physiological role in different organs. The present study was designed to elucidate the antioxidant and free radical scavenging potentials of L-arginnine (a source for endogenous production of NO in vivo) and NaHS (a source H2S) individually and in combination. Different assays like 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging, percent inhibition of linoleic acid peroxidation and reducing power assays were used to evaluate the free radical scavenging capacity and antioxidant activity of L-arginine and NaHS. Furthermore, study was aimed to know the antioxidant potential of both compounds at their effective doses in human body, which is 56 µM for H2S and 1.2 g/mL for L-arginine. The study also aimed to clear whether either NaHS, L-arginine or the mixture of NaHS and L-arginine in vitio (in the form of new compounds) is responsible for their therapeutic action. Results showed that NaHS, L-arginine and combination of NaHS + L-arginine showed good radical scavenging activity i.e., 55.60%, 52.10% and 52.32%, respectively. Moreover, NaHS was found to have ability to inhibit linoleic acid peroxidation by 53.98% at effective dose while L-arginine did not show inhibition of linoleic acid peroxidation. Combination of NaHS + L-arginine showed 54.15% inhibition of linoleic acid peroxidation, which is similar to that of H2S. Reducing power of NaHS was 0.073 and L-arginine showed 0.037, combination of NaHS + L-arginine showed 0.063. It can be concluded that NaHS showed better antioxidant potential in vitio as compared to L-arginine and the antioxidant activity of the mixture of NaHS + L-arginine is closed to the antioxidant activity of NaHS, which reflects that NaHS is a dominant factor in combination mixture that is responsible for antioxidant activity.
authors.forename: Ashfaq
authors.initials: A
authors.lastname: Ahmad
authors.forename: Munavvar Zubaid A
authors.initials: MZ
authors.lastname: Sattar
authors.forename: Hassaan A
authors.initials: HA
authors.lastname: Rathore
authors.forename: Abdullah I
authors.initials: AI
authors.lastname: Hussain
authors.forename: Safia Akhtar
authors.initials: SA
authors.lastname: Khan
authors.forename: Tabinda
authors.initials: T
authors.lastname: Fatima
authors.forename: Sheryar
authors.initials: S
authors.lastname: Afzal
authors.forename: Nor A
authors.initials: NA
authors.lastname: Abdullah
authors.forename: Edward J
authors.initials: EJ
authors.lastname: Johns
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D001120:Arginine / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000494:pharmacology*; D006801:Humans; D013440:Sulfides / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D016166:Free Radical Scavengers; D013440:Sulfides; D001120:Arginine; C025451:sodium bisulfide