title: Selenium speciation in paired serum and cerebrospinal fluid samples of sheep.
abstract: This study was performed to characterise selenium (Se) and Se species in cerebrospinal fluid (CSF) of sheep and its relation to the respective Se concentrations in serum. Paired samples from 10 adult sheep were used for the study. Five sheep were fed a diet with a marginal Se concentration of <0.05mg Se/kg diet dry weight (dw, Se(-)), and five animals were fed the same diet supplemented with sodium selenite revealing a concentration of 0.2mg Se/kg diet dw (Se(+)). The feeding strategy was conducted for two years; The results on metabolic effects were published previously. At the end of the feeding period, paired samples of serum and CSF were collected and analysed using ion exchange chromatography inductively coupled plasma-dynamic reaction cell-mass spectrometry (IEC-ICP-DRC-MS) technique for total Se concentration and concentrations of Se species. Albumin concentrations were analysed additionally. The feeding strategy caused significant differences (p<0.01) in serum Se concentrations with 33.1±5.11μg Se/l in the Se(-) group and 96.5±18.3μg Se/l in the Se(+) group, respectively. The corresponding total Se concentrations in CSF were 4.38±1.02μg Se/l and 6.13±1.64μg Se/l in the Se(-) and the Se(+) group, respectively, missing statistical significance (p=0.077). IEC-ICP-DRC-MS technique was able to differentiate the Se species selenoprotein P-bound Se (SePP), selenomethionine, glutathione peroxidase-bound Se (Se-GPx), selenocystine, thioredoxin reductase-bound Se, ovine serum albumin-bound Se (Se-OSA), SeIV and SeVI in ovine serum and CSF. Quantitatively, SePP is the main selenoprotein in ovine serum followed by Se-GPx. The CSF/blood ratio of albumin (QAlbumin) reflected a physiological function of the blood-CSF barrier in all sheep. QSe-species were higher than QAlbumin both feeding groups, supporting the hypothesis of local production of Se species in the brain. Significant positive regression lines for CSF vs. serum were found for albumin and Se-OSA only, suggesting a role of albumin to convey Se across the blood-CSF barrier. The ovine model, together with the IEC-ICP-DRC-MS technique to characterise the Se species, might be a worthwhile model for further studies as repeated sample collection as well as modification of the nutritional status is feasible and effective.
authors.affiliation: Klinik für kleine Klauentiere und Forensische Medizin und Ambulatorische Klinik, Stiftung Tierärztliche Hochschule Hannover, Bischofsholer Damm 15, D-30173 Hannover, Germany. Electronic address: esther.humann-ziehank@tiho-hannover.de.
authors.forename: Esther
authors.initials: E
authors.lastname: Humann-Ziehank
authors.affiliation: Klinik für kleine Klauentiere und Forensische Medizin und Ambulatorische Klinik, Stiftung Tierärztliche Hochschule Hannover, Bischofsholer Damm 15, D-30173 Hannover, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Ganter
authors.affiliation: Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt Research Unit Analytical BioGeoChemistry, Ingolstädter Landstraße 1, D-85764 Neuherberg, München, Germany.
authors.forename: Bernhard
authors.initials: B
authors.lastname: Michalke
mesh_terms: D000818:Animals; D005979:Glutathione Peroxidase / Q000378:metabolism; D006801:Humans; D012643:Selenium / Q000097:blood* / Q000134:cerebrospinal fluid*; D051149:Selenoprotein P / Q000378:metabolism; D012709:Serum Albumin / Q000378:metabolism; D012756:Sheep / Q000097:blood* / Q000134:cerebrospinal fluid*; D013880:Thioredoxin-Disulfide Reductase / Q000378:metabolism
chemical_list: D051149:Selenoprotein P; D012709:Serum Albumin; D005979:Glutathione Peroxidase; D013880:Thioredoxin-Disulfide Reductase; D012643:Selenium
keywords: Cerebrospinal fluid; IEC–ICP–DRC–MS; Selenium; Selenoproteins; Serum; Sheep