title: Development of microsatellite loci for Taxus wallichiana var. wallichiana (Taxaceae) and cross-amplification in Taxaceae.
abstract: Nine polymorphic microsatellite loci were isolated and characterized in Taxus wallichiana var. wallichiana, an endangered species in China. The number of alleles per locus ranged from 2 to 20. Observed and expected heterozygosities varied from 0.0260 to 0.5325 and 0.3603 to 0.9231, respectively. Positive cross-amplification of the 9 loci was observed in 2 other varieties of T. wallichiana and 4 other Taxaceae species. These loci will be of value for studying population genetic structures and for genetic resource conservation in T. wallichiana and other Taxus species.
authors.affiliation: State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Silviculture of the State Forestry Administration, Lab of Molecular Identification of Plant Varieties, Research Institute of Forestry, Chinese Academy of Forestry, Haidian District, Beijing, China.
authors.forename: B B
authors.initials: BB
authors.lastname: Cheng
authors.affiliation: State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Silviculture of the State Forestry Administration, Lab of Molecular Identification of Plant Varieties, Research Institute of Forestry, Chinese Academy of Forestry, Haidian District, Beijing, China.
authors.forename: Q W
authors.initials: QW
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Silviculture of the State Forestry Administration, Lab of Molecular Identification of Plant Varieties, Research Institute of Forestry, Chinese Academy of Forestry, Haidian District, Beijing, China.
authors.forename: Y Q
authors.initials: YQ
authors.lastname: Zheng
mesh_terms: D000483:Alleles; D056727:Endangered Species; D056426:Genetic Loci; D005828:Genetics, Population; D018895:Microsatellite Repeats; D011110:Polymorphism, Genetic; D020946:Taxus / Q000145:classification / Q000235:genetics*