title: Use of Lanthanide-Containing Polyoxometalates to Sensitise the Emission of Fluorescent Labelled Serum Albumin.
abstract: Monitoring the interaction of biomolecules is important, and the use of energy transfer is a principal technique in elucidating nanoscale interactions. Lanthanide compounds are promising luminescent probes for biological samples as their emission is longer-lived than any native autofluorescence. Polyoxometalates (POMs) are interesting structural motifs to incorporate lanthanides, offering low toxicity and a size pertinent for biological applications. Here, we employ iso-structured POMs containing either terbium or europium and assess their interaction with serum albumin by sensitisation of a fluorescent tag on the protein via LRET (luminescence resonance energy transfer) by exciting the lanthanide. Time-resolved measurements showed energy transfer with an efficiency of over 90% for the POM-protein systems. The Tb-POM results were relatively straightforward, while those with the iso-structured Eu-POM were complicated by the effect of protein shielding from the aqueous environment.
authors.affiliation: School of Engineering and Built Environment, Glasgow Caledonian University, Cowcaddens Road, Glasgow, G4 0BA, UK.
authors.forename: A Sheila
authors.initials: AS
authors.lastname: Holmes-Smith
authors.affiliation: School of Engineering and Built Environment, Glasgow Caledonian University, Cowcaddens Road, Glasgow, G4 0BA, UK.
authors.forename: Jacob
authors.initials: J
authors.lastname: Crisp
authors.affiliation: Department of Chemistry, University of Delhi, Delhi -, 110007, India.
authors.forename: Firasat
authors.initials: F
authors.lastname: Hussain
authors.affiliation: Department of Chemistry, University of Zurich, Winterthurerstrasse 190, CH-8057, Zurich, Switzerland.
authors.forename: Greta R
authors.initials: GR
authors.lastname: Patzke
authors.affiliation: HORIBA Jobin Yvon IBH Ltd, 133 Finnieston Street, Glasgow, G3 8HB, UK. graham.hungerford@horiba.com.
authors.forename: Graham
authors.initials: G
authors.lastname: Hungerford
mesh_terms: D004735:Energy Transfer; D005453:Fluorescence; D031541:Fluorescence Resonance Energy Transfer; D006801:Humans; D028581:Lanthanoid Series Elements / Q000737:chemistry*; D012709:Serum Albumin / Q000737:chemistry*; D017973:Tungsten Compounds / Q000737:chemistry*; D000099294:Polyoxometalates
chemical_list: D028581:Lanthanoid Series Elements; D012709:Serum Albumin; D017973:Tungsten Compounds; C102296:polyoxometalate I; D000099294:Polyoxometalates
keywords: LRET; europium; protein; terbium; time-resolved luminescence