title: The diameter of nanotubes formed on Ti-6Al-4V alloy controls the adhesion and differentiation of Saos-2 cells.
abstract: Ti-6Al-4V-based nanotubes were prepared on a Ti-6Al-4V surface by anodic oxidation on 10 V, 20 V, and 30 V samples. The 10 V, 20 V, and 30 V samples and a control smooth Ti-6Al-4V sample were evaluated in terms of their chemical composition, diameter distribution, and cellular response. The surfaces of the 10 V, 20 V, and 30 V samples consisted of nanotubes of a relatively wide range of diameters that increased with the voltage. Saos-2 cells had a similar initial adhesion on all nanotube samples to the control Ti-6Al-4V sample, but it was lower than on glass. On day 3, the highest concentrations of both vinculin and talin measured by enzyme-linked immunosorbent assay and intensity of immunofluorescence staining were on 30 V nanotubes. On the other hand, the highest concentrations of ALP, type I collagen, and osteopontin were found on 10 V and 20 V samples. The final cellular densities on 10 V, 20 V, and 30 V samples were higher than on glass. Therefore, the controlled anodization of Ti-6Al-4V seems to be a useful tool for preparing nanostructured materials with desirable biological properties.
authors.affiliation: Department of Biomaterials and Tissue Engineering, Institute of Physiology, Czech Academy of Sciences, Prague, Czech Republic.
authors.forename: Elena
authors.initials: E
authors.lastname: Filova
authors.affiliation: Department of Metals and Corrosion Engineering, University of Chemistry and Technology, Prague, Czech Republic.
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Fojt
authors.affiliation: Department of Biomaterials and Tissue Engineering, Institute of Physiology, Czech Academy of Sciences, Prague, Czech Republic.
authors.forename: Marketa
authors.initials: M
authors.lastname: Kryslova
authors.affiliation: Department of Metals and Corrosion Engineering, University of Chemistry and Technology, Prague, Czech Republic.
authors.forename: Hynek
authors.initials: H
authors.lastname: Moravec
authors.affiliation: Department of Metals and Corrosion Engineering, University of Chemistry and Technology, Prague, Czech Republic.
authors.forename: Ludek
authors.initials: L
authors.lastname: Joska
authors.affiliation: Department of Biomaterials and Tissue Engineering, Institute of Physiology, Czech Academy of Sciences, Prague, Czech Republic.
authors.forename: Lucie
authors.initials: L
authors.lastname: Bacakova
mesh_terms: D000199:Actins / Q000378:metabolism; D000497:Alloys; D015415:Biomarkers / Q000378:metabolism; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D005455:Fluorescent Antibody Technique; D006801:Humans; D043942:Nanotubes / Q000737:chemistry*; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects; D010012:Osteogenesis / Q000187:drug effects; D010316:Particle Size; D056951:Photoelectron Spectroscopy; D013499:Surface Properties; D014025:Titanium / Q000494:pharmacology*; D016596:Vinculin / Q000378:metabolism
chemical_list: D000199:Actins; D000497:Alloys; D015415:Biomarkers; D016596:Vinculin; C031462:titanium alloy (TiAl6V4); D014025:Titanium
keywords: Saos-2 cells; cell adhesion; nanostructure; osteogenic differentiation; titanium nanotubes