title: Identification and molecular characterization of Panax notoginseng virus A, which may represent an undescribed novel species of the genus Totivirus, family Totiviridae.
abstract: A double-stranded RNA (dsRNA), PN-S, isolated from Panax notoginseng was sequenced and characterized. dsRNA PN-S was determined to be 5003 base pairs (bp) in length and to have a genome organization similar to that of totiviruses. The two deduced proteins encoded by dsRNA PN-S have the highest amino acid (aa) sequence identity to CP and RNA-dependent RNA polymerase (RdRp) of black raspberry virus F (BrVF), a tentative member of the genus Totivirus, family Totiviridae, at 39% and 54%, respectively. In a phylogenetic tree based on an alignment of the RdRp amino acid sequences of dsRNA PN-S and selected totiviruses, dsRNA PN-S formed a monophyletic clade with dsRNA viruses belonging to the genus Totivirus. These data suggest that dsRNA PN-S may represent the genome of a member of a novel species of the genus Totivirus, family Totiviridae, for which we propose the name Panax notoginseng virus A (PnVA).
authors.affiliation: Engineering Research Centre of Ecology and Agricultural Use of Wetland, Ministry of Education, Yangtze University, Jingzhou, 434025, Hubei, China.
authors.forename: Lingfang
authors.initials: L
authors.lastname: Guo
authors.affiliation: Key Laboratory of Agro-Biodiversity and Pest Management of Education Ministry of China, Yunnan Agricultural University, Kunming, 650201, Yunnan, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Yang
authors.affiliation: Key Laboratory of Agro-Biodiversity and Pest Management of Education Ministry of China, Yunnan Agricultural University, Kunming, 650201, Yunnan, China.|Fujian Province Key Laboratory of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wu
authors.affiliation: Modern Education Technology Centre, Office of Teaching Affairs, Yunnan Agricultural University, Kunming, 650201, Yunnan, China.
authors.forename: Guanlin
authors.initials: G
authors.lastname: Tan
authors.affiliation: Engineering Research Centre of Ecology and Agricultural Use of Wetland, Ministry of Education, Yangtze University, Jingzhou, 434025, Hubei, China.
authors.forename: Shouguo
authors.initials: S
authors.lastname: Fang
authors.affiliation: Engineering Research Centre of Ecology and Agricultural Use of Wetland, Ministry of Education, Yangtze University, Jingzhou, 434025, Hubei, China. yangtze2008@126.com.|Fujian Province Key Laboratory of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, China. yangtze2008@126.com.
authors.forename: Songbai
authors.identifier: http://orcid.org/0000-0002-8425-1434
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Agro-Biodiversity and Pest Management of Education Ministry of China, Yunnan Agricultural University, Kunming, 650201, Yunnan, China. fanlikm@126.com.
authors.forename: Fan
authors.initials: F
authors.lastname: Li
mesh_terms: D036022:Capsid Proteins / Q000235:genetics; D016000:Cluster Analysis; D023061:Gene Order; D016679:Genome, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D053723:Panax notoginseng / Q000821:virology*; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics*; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D019196:Totiviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D036022:Capsid Proteins; D012367:RNA, Viral; D012324:RNA-Dependent RNA Polymerase