title: [Effect of chronic aluminum exposure on neuron apoptosis and expression of P53 phosphorylation in rats].
abstract: OBJECTIVE
  To explore the role of P53 phosphorylation in neuron apoptosis of rats by chronic aluminum exposure.
  
  
  METHODS
  A total of male 40 SD rats were divided randomly into 4 groups (n = 10/dose), the exposed groups were fed with normal diet with different concentration of AlCl3 · 6H2O for 6 months respectively. The dosage of low, middle and high groups were 10.73, 107.33, 1073.33 mg/kg in sequence. The control group received normal diet. The neuron apoptosis was measured by method of Tunel. The expressions of P53 and pP53-ser15 protein in the cortex were detected by Western-blot.
  
  
  RESULTS
  Tunel staining showed that the low, middle and high group rats had increased apoptosis rate than control group (P < 0.01). Western-blot test demonstrated that the expression of P53 protein in the cortex of high group rats were significantly higher than the control and low groups (P < 0.05). The expression of pP53-ser15 protein in the cortex of middle and high group rats were also higher than the control and low groups (P < 0.05).
  
  
  CONCLUSIONS
  Chronic aluminum exposure can lead to over expression of P53 and pP53-ser15 protein in cerebral cortex, which maybe one of the most important mechanisms of neuron apoptosis induced by AlCl3.
authors.affiliation: Taigang Center for Disease Control and Prevention, Taiyuan 030003, China.
authors.forename: Baolong
authors.initials: B
authors.lastname: Pan
authors.forename: Shuandong
authors.initials: S
authors.lastname: Guo
authors.forename: Liang
authors.initials: L
authors.lastname: Guo
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.affiliation: E-mail: niuqiao55@163.com.
authors.forename: Qiao
authors.initials: Q
authors.lastname: Niu
mesh_terms: D000535:Aluminum / Q000633:toxicity*; D000077410:Aluminum Chloride; D017607:Aluminum Compounds / Q000633:toxicity; D000818:Animals; D017209:Apoptosis; D002540:Cerebral Cortex / Q000378:metabolism; D002712:Chlorides / Q000633:toxicity; D008297:Male; D009474:Neurons / Q000166:cytology / Q000187:drug effects*; D010766:Phosphorylation; D051381:Rats; D017207:Rats, Sprague-Dawley; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
chemical_list: D017607:Aluminum Compounds; D002712:Chlorides; D016159:Tumor Suppressor Protein p53; D000077410:Aluminum Chloride; D000535:Aluminum