title: Infusion of basic fibroblast growth factor ameliorates acute graft versus host disease in mice after haploidentical hematopoietic stem cell transplantation.
abstract: The aim of this study was to investigate the effect of basic fibroblast growth factor (bFGF) on acute graft versus host disease (aGVHD) in mice after haploidentical hematopoietic stem cell transplantation (haplo-HSCT). Haplo-HSCT mice model was established followed by dividing into three groups with 12 mice in each group, group 1 with infusion of 100g/kg bFGF, group 2 with infusion of 20 g/kg bFGF and control group without infusion. Clinical manifestation and survival time of mice after haplo-HSCT were monitored. On day 14 post transplantation, mice were sacrificed for pathology analysis of liver and the changes of mesenchymal stem cells (MSC). Compared to haplo-HSCT group, clinical manifestations of aGVHD in bFGF infusion group were significantly ameliorated. Furthermore, bFGF infusion also significantly prolonged the survival time of mice after transplantation (P<0.05) as demonstrated by Kaplan-Meier survival analysis with more infusion of bFGF, the longer survival of mice. Pathology analysis showed the severity of aGVHD in bFGF infusion group (1 and 2) was less severe than haplo-HSCT group with higher proliferation of bone marrow MSC in group 1. In conclusion, these studies demonstrated that infusion of bFGF ameliorated aGVHD in mice after haplo-HSCT.
authors.affiliation: Department of Hematology, The ChengDu Military General Hospital, China.
authors.forename: Fangyi
authors.initials: F
authors.lastname: Fan
authors.affiliation: Department of Hematology, The ChengDu Military General Hospital, China.
authors.forename: Li
authors.initials: L
authors.lastname: Fu
authors.affiliation: Department of Hematology, The ChengDu Military General Hospital, China.
authors.forename: Guangcui
authors.initials: G
authors.lastname: He
authors.affiliation: Department of Hematology, The ChengDu Military General Hospital, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Deng
authors.affiliation: Department of Hematology, The ChengDu Military General Hospital, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Qiu
authors.affiliation: Department of Hematology, The ChengDu Military General Hospital, China.
authors.forename: Yilan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Hematology, The ChengDu Military General Hospital, China.
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Miao
authors.affiliation: Department of Hematology, The ChengDu Military General Hospital, China.
authors.forename: Yanrong
authors.initials: Y
authors.lastname: Shuai
authors.affiliation: Department of Hematology, The ChengDu Military General Hospital, China. Electronic address: yisu804@sina.com.
authors.forename: Yi
authors.initials: Y
authors.lastname: Su
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects / Q000378:metabolism; D002454:Cell Differentiation; D004195:Disease Models, Animal; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000008:administration & dosage*; D006086:Graft vs Host Disease / Q000175:diagnosis / Q000188:drug therapy* / Q000209:etiology* / Q000401:mortality; D018380:Hematopoietic Stem Cell Transplantation / Q000009:adverse effects; D007958:Leukocyte Count; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D010641:Phenotype; D014184:Transplantation, Homologous
chemical_list: D016222:Fibroblast Growth Factor 2
keywords: Acute graft versus host disease; Basic fibroblast growth factor; Hematopoietic stem cell transplantation