title: Determination of Menthol in Plasma and Urine by Gas Chromatography/Mass Spectrometry (GC/MS).
abstract: Menthol, a monoterpene, is a principal component of peppermint oil and is used extensively in consumer products as a flavoring aid. It is also commonly used medicinally as a topical skin coolant; to treat inflammation of the mucous membranes, digestive problems, and irritable bowel syndrome (IBS); and in preventing spasms during endoscopy and for its spasmolytic effect on the smooth muscle of the gastrointestinal tract. Menthol has a half life of 3-6 h and is rapidly metabolized to menthol glucuronide which is detectable in urine and serum following menthol use. We describe a method for the determination of total menthol in human plasma and urine using liquid/liquid extraction, gas chromatography/mass spectrometry (GC/MS) in selected ion monitoring mode and menthol-d4 as the internal standard. Controls are prepared with menthol glucuronide and all samples undergo enzymatic hydrolysis for the quantification of total menthol. The method has a linear range of 5-1000 ng/mL, and coefficient of variation <10%.
authors.affiliation: Department of Pathology and Laboratory Medicine, Children's Mercy Hospitals and Clinics, 2401 Gillham Road, Kansas City, MO, 64108, USA.
authors.forename: Judy
authors.initials: J
authors.lastname: Peat
authors.affiliation: Department of Pathology and Laboratory Medicine, Children's Mercy Hospitals and Clinics, 2401 Gillham Road, Kansas City, MO, 64108, USA.
authors.forename: Clint
authors.initials: C
authors.lastname: Frazee
authors.affiliation: Department of Pathology and Laboratory Medicine, Children's Mercy Hospitals and Clinics, 2401 Gillham Road, Kansas City, MO, 64108, USA.|Department of Pediatrics, Children's Mercy Hospitals and Clinics, 2401 Gillham Road, Kansas City, MO, 64108, USA.
authors.forename: Gregory
authors.initials: G
authors.lastname: Kearns
authors.affiliation: Department of Pathology and Laboratory Medicine, Children's Mercy Hospitals and Clinics, 2401 Gillham Road, Kansas City, MO, 64108, USA. ugarg@cmh.edu.
authors.forename: Uttam
authors.initials: U
authors.lastname: Garg
mesh_terms: D000982:Antipruritics / Q000097:blood* / Q000652:urine*; D005421:Flavoring Agents / Q000493:pharmacokinetics; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D043183:Irritable Bowel Syndrome / Q000188:drug therapy; D059625:Liquid-Liquid Extraction / Q000379:methods; D036142:Mentha piperita; D008610:Menthol / Q000097:blood* / Q000652:urine*; D010938:Plant Oils / Q000737:chemistry
chemical_list: D000982:Antipruritics; D005421:Flavoring Agents; D010938:Plant Oils; D008610:Menthol; C015424:peppermint oil
keywords: GCMS; Irritable bowel syndrome; Menthol; Peppermint oil