title: Preparation of nano/macroporous polycaprolactone microspheres for an injectable cell delivery system using room temperature ionic liquid and camphene.
abstract: The nano/macroporous polycaprolactone (PCL) microspheres with cell active surfaces were developed as an injectable cell delivery system. Room temperature ionic liquid (RTIL) and camphene were used as a liquid mold and a porogen, respectively. Various-sized spheres of 244-601μm with pores of various size and shape of 0.02-100μm, were formed depending on the camphene/RTIL ratio (0.8-2.6). To give cell activity, the surface of porous microspheres were further modified with nerve growth factors (NGF) containing gelatin to give a thin NGF/gelatin layer, to which the neural progenitor cells (PC-12) attached and extended their neurites on to the surface layers of the microspheres. The developed microspheres may be potentially applicable as a neuronal cell delivery scaffold for neuron tissue engineering.
authors.affiliation: Department of Nanobiomedical Science & BK21 PlUS NBM Global Research Center for Regenerative Medicine, Dankook University, Dandae-ro, Dongnam-gu, Cheonan-si, Chungnam 330-714, Republic of Korea; Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Dandae-ro, Dongnam-gu, Cheonan-si, Chungnam 330-714, Republic of Korea.
authors.forename: Seong Yeol
authors.initials: SY
authors.lastname: Kim
authors.affiliation: Department of Biomedical Engineering, Korea University, 145 Anam-ro, Seongbuk-gu, Seoul 136-713, Republic of Korea.
authors.forename: Ji-Young
authors.initials: JY
authors.lastname: Hwang
authors.affiliation: Department of Nanobiomedical Science & BK21 PlUS NBM Global Research Center for Regenerative Medicine, Dankook University, Dandae-ro, Dongnam-gu, Cheonan-si, Chungnam 330-714, Republic of Korea; Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Dandae-ro, Dongnam-gu, Cheonan-si, Chungnam 330-714, Republic of Korea. Electronic address: usshin12@dankook.ac.kr.
authors.forename: Ueon Sang
authors.initials: US
authors.lastname: Shin
mesh_terms: D000818:Animals; D000081008:Bicyclic Monoterpenes; D052578:Ionic Liquids / Q000737:chemistry*; D008863:Microspheres; D058608:Nanopores; D020932:Nerve Growth Factor / Q000737:chemistry*; D058953:Neural Stem Cells / Q000166:cytology / Q000378:metabolism; D016716:PC12 Cells; D011091:Polyesters / Q000737:chemistry*; D051381:Rats; D013729:Terpenes / Q000737:chemistry*
chemical_list: D000081008:Bicyclic Monoterpenes; D052578:Ionic Liquids; D011091:Polyesters; D013729:Terpenes; C016240:polycaprolactone; D020932:Nerve Growth Factor; C019286:camphene
keywords: An injectable cell delivery system; NGF-loaded microspheres; Nano- and micro-porous surface morphology; Porous PCL microspheres; Room temperature ionic liquids