title: Simultaneous Quantitation of Lamotrigine, Levetiracetam, 10-Hydroxycarbazepine, Topiramate, and Zonisamide in Serum Using HPLC-MS/MS.
abstract: Antiepileptic drugs (AEDs) are a diverse group of pharmacological agents used in the treatment of epileptic seizures. Over the past several decades some new AEDs, including lamotrigine (LTG), levetiracetam (LVA), oxcarbazepine (OXC), topiramate (TOP), and zonisamide (ZNS), have become widely used. This chapter describes a very simple and rapid liquid chromatography-tandem mass spectrometry method for simultaneous quantitation of LVA, ZNS, LTG, TOP, and MHD in human serum. The method requires a very small amount of serum (50 μL) for multiple drug measurements and has a total analysis time of 4 min that makes it well suited for routine clinical analysis of several drugs simultaneously. The imprecision (CVs) measured at various concentrations across the analytical measurement range (AMR) are less than 7% for all analytes. The AMR for each analyte is as follows: LVA (1-100 μg/mL), ZNS (0.8-80 μg/mL), TOP (0.5-50 μg/mL), and 0.6-60 μg/mL for LTG and MHD.
authors.affiliation: Department of Laboratory Medicine, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY, 10065, USA. carlowd@mskcc.org.
authors.forename: Dean C
authors.initials: DC
authors.lastname: Carlow
authors.affiliation: The Department of Pathology and Laboratory Medicine, Children'sHospital of Philadelphia, 34th St. and Civic Center Blvd., Philadelphia, PA, 19104, USA.
authors.forename: Heng
authors.initials: H
authors.lastname: Shi
authors.affiliation: Department of Laboratory Medicine, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY, 10065, USA.
authors.forename: Ryan C
authors.initials: RC
authors.lastname: Schofield
mesh_terms: D000927:Anticonvulsants / Q000097:blood*; D002220:Carbamazepine / Q000031:analogs & derivatives* / Q000097:blood; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D016903:Drug Monitoring / Q000379:methods*; D005632:Fructose / Q000031:analogs & derivatives* / Q000097:blood; D006801:Humans; D007555:Isoxazoles / Q000097:blood*; D000077213:Lamotrigine; D000077287:Levetiracetam; D010889:Piracetam / Q000031:analogs & derivatives* / Q000097:blood; D053719:Tandem Mass Spectrometry / Q000379:methods; D000077236:Topiramate; D014227:Triazines / Q000097:blood*; D000078305:Zonisamide
chemical_list: C039775:10,11-dihydro-10-hydroxycarbamazepine; D000927:Anticonvulsants; D007555:Isoxazoles; D014227:Triazines; D000077236:Topiramate; D005632:Fructose; D002220:Carbamazepine; D000077287:Levetiracetam; D000078305:Zonisamide; D000077213:Lamotrigine; D010889:Piracetam
keywords: 10-Hydroxycarbazepine; Antiepileptic drugs; Lamotrigine; Levetiracetam; Mass spectrometry; Therapeutic drug monitoring; Topiramate; Zonisamide