title: The preliminary study of immune superparamagnetic iron oxide nanoparticles for the detection of lung cancer in magnetic resonance imaging.
abstract: To improve the sensitive and specific detection of metastasis of lung cancer, this study fabricated immune superparamagnetic iron oxide nanoparticles (SPIONs) used in magnetic resonance (MR) immumoimaging. These SPIONs were coated with oleic acid and carboxymethyl dextran, and then conjugated to mouse anti-CD44v6 monoclonal antibody. The physicochemical properties of magnetic nanoparticles without monoclonal antibody were characterized by X-ray powder diffraction (XRD), thermogravimetric analysis (TGA), and Fourier transform infrared spectroscopy (FTIR). The sizes of the nanoparticles were determined by dynamic light scattering measurements (DLS) and transmission electron microscope (TEM). Coated nanoparticles could well disperse in water with low dosage of CMD as the Fe/CMD ratio is 1/1 and 2/1 (w/w). Importantly, these SPIONs have relatively high saturation magnetization, as measured by vibrating sample magnetometer (VSM). They could efficiently become the transversal relaxation times (T2) contrast agent to improve detection limit through measured in vitro magnetic resonance imaging (MRI) and actively target human lung adenocarcinoma (A549) cells in vitro cell culture. Thus, these immune SPIONs are potentially useful for lung tumor-targeting diagnosis.
authors.affiliation: College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu 610065, China.
authors.forename: Xinyuan
authors.initials: X
authors.lastname: Wan
authors.affiliation: College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu 610065, China.
authors.forename: Yuanqing
authors.initials: Y
authors.lastname: Song
authors.affiliation: College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu 610065, China.
authors.forename: Nijia
authors.initials: N
authors.lastname: Song
authors.affiliation: College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu 610065, China. Electronic address: jiehua_li@scu.edu.cn.
authors.forename: Jiehua
authors.initials: J
authors.lastname: Li
authors.affiliation: Institute of Digestive Surgery and State key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, China.
authors.forename: Lie
authors.initials: L
authors.lastname: Yang
authors.affiliation: Department of Radiology, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, China. Electronic address: dr.liyuan@163.com.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Li
authors.affiliation: College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu 610065, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Tan
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology; D045744:Cell Line, Tumor; D003911:Dextrans / Q000737:chemistry; D004355:Drug Stability; D006801:Humans; D018960:Hyaluronan Receptors / Q000276:immunology; D018796:Immunoconjugates / Q000737:chemistry*; D008175:Lung Neoplasms / Q000175:diagnosis* / Q000473:pathology; D008279:Magnetic Resonance Imaging / Q000379:methods*; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D051379:Mice; D014867:Water / Q000737:chemistry
chemical_list: D000911:Antibodies, Monoclonal; C112697:CD44v6 antigen; D003911:Dextrans; D018960:Hyaluronan Receptors; D018796:Immunoconjugates; D058185:Magnetite Nanoparticles; D014867:Water; C014392:carboxymethyl dextran
keywords: Active target; Carboxymethyl dextran; Oleic acid; SPIONs