title: Escherichia coli Vertebral Osteomyelitis Diagnosed According to Broad-range 16S rRNA Gene Polymerase Chain Reaction (PCR).
abstract: Identifying the causative agent of pyogenic osteomyelitis is often challenging, especially when antibiotics are administered before a biopsy. We herein present a case of osteomyelitis in the cervical vertebrae presenting with progressive paralytic symptoms, in which we successfully identified Escherichia coli from a biopsy specimen using broad-range 16S rRNA gene polymerase chain reaction (PCR) even though sensitive antibiotics had been used for more than 50 days before the biopsy. Broad-range 16S rRNA gene PCR is a useful diagnostic method, especially when prebiopsy antibiotics are unavoidably used for a clinically unstable state.
authors.affiliation: AIDS Clinical Center, National Center for Global Health and Medicine, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Shibata
authors.forename: Ryutaro
authors.initials: R
authors.lastname: Tanizaki
authors.forename: Koji
authors.initials: K
authors.lastname: Watanabe
authors.forename: Kenta
authors.initials: K
authors.lastname: Makabe
authors.forename: Naoki
authors.initials: N
authors.lastname: Shoda
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kutsuna
authors.forename: Maki
authors.initials: M
authors.lastname: Nagamatsu
authors.forename: Shinichi
authors.initials: S
authors.lastname: Oka
authors.forename: Norio
authors.initials: N
authors.lastname: Ohmagari
mesh_terms: D000900:Anti-Bacterial Agents; D002574:Cervical Vertebrae; D004756:Enterobacteriaceae Infections / Q000175:diagnosis*; D004926:Escherichia coli / Q000235:genetics*; D005260:Female; D006801:Humans; D008875:Middle Aged; D010019:Osteomyelitis / Q000175:diagnosis*; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D012336:RNA, Ribosomal, 16S