title: Fusogenic Reactive Oxygen Species Triggered Charge-Reversal Vector for Effective Gene Delivery.
abstract: A novel fusogenic lipidic polyplex (FLPP) vector is designed to fuse with cell membranes, mimicking viropexis, and eject the polyplex into the cytosol, where the cationic polymer is subsequently oxidized by intracellular reactive oxygen species and converts to being negatively charged, efficiently releasing the DNA. The vector delivering suicide gene achieves significantly better inhibition of tumor growth than doxorubicin.
authors.affiliation: Center for Bionanoengineering and Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310027, China.|Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, 310027, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.affiliation: Center for Bionanoengineering and Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310027, China.
authors.forename: Jiajia
authors.initials: J
authors.lastname: Xiang
authors.affiliation: Center for Bionanoengineering and Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310027, China.
authors.forename: Dingcheng
authors.initials: D
authors.lastname: Zhu
authors.affiliation: Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, 310027, China.
authors.forename: Liming
authors.initials: L
authors.lastname: Jiang
authors.affiliation: Center for Bionanoengineering and Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310027, China.
authors.forename: Zhuxian
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: Center for Bionanoengineering and Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310027, China.
authors.forename: Jianbin
authors.initials: J
authors.lastname: Tang
authors.affiliation: Center for Bionanoengineering and Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310027, China.
authors.forename: Xiangrui
authors.initials: X
authors.lastname: Liu
authors.affiliation: Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203, China.
authors.forename: Yongzhuo
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Center for Bionanoengineering and Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310027, China.
authors.forename: Youqing
authors.initials: Y
authors.lastname: Shen
mesh_terms: D001692:Biological Transport; D045744:Cell Line, Tumor; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004337:Drug Carriers / Q000737:chemistry* / Q000378:metabolism; D018014:Gene Transfer Techniques; D006801:Humans; D042541:Intracellular Space / Q000378:metabolism; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D010084:Oxidation-Reduction; D011108:Polymers / Q000737:chemistry* / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*
chemical_list: D004337:Drug Carriers; D011108:Polymers; D017382:Reactive Oxygen Species; D004247:DNA
keywords: ROS-responsive; charge reversal polymers; fusogenic lipid vectors; gene delivery; phenylboronic acid (PBA)