title: Type 1 Diabetes High-Risk HLA Class II Determination by Polymerase Chain Reaction Sequence-Specific Primers.
abstract: The only strategy to select individuals at increased risk for type 1 diabetes for primary prevention trials is through genetic risk assessment. While genome-wide association studies have identified more than 40 loci associated with type 1 diabetes, the single most important genetic determinants lie within the human leucocyte antigen gene family on chromosome 6.In this chapter we describe a protocol for a straightforward, cheap strategy to determine HLA class II mediated risk of type 1 diabetes. This method has proved robust for genotyping whole-genome-amplified DNA as well as DNA extracted directly from human tissues.
authors.affiliation: Diabetes and Metabolism Unit, School of Clinical Sciences, Southmead Hospital, University of Bristol, Bristol, UK.
authors.forename: Rachel J
authors.initials: RJ
authors.lastname: Aitken
authors.affiliation: Diabetes and Metabolism Unit, School of Clinical Sciences, Southmead Hospital, University of Bristol, Bristol, UK.
authors.forename: Georgina L
authors.initials: GL
authors.lastname: Mortimer
authors.affiliation: Diabetes and Metabolism Unit, School of Clinical Sciences, Southmead Hospital, University of Bristol, Bristol, UK. K.M.Gillespie@bristol.ac.uk.
authors.forename: Kathleen M
authors.initials: KM
authors.lastname: Gillespie
mesh_terms: D017931:DNA Primers; D003922:Diabetes Mellitus, Type 1 / Q000175:diagnosis* / Q000235:genetics; D000949:Histocompatibility Antigens Class II / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012307:Risk Factors; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D017931:DNA Primers; D000949:Histocompatibility Antigens Class II
keywords: Genetic risk; HLA class 1; HLA class II