title: EliA is required for inducing the stearyl alcohol-mediated expression of secretory proteins and production of polyester in Ralstonia sp. NT80.
abstract: Addition of stearyl alcohol to the culture medium of Ralstonia sp. NT80 induced expression of a significant amount of secretory lipase. Comparative proteomic analysis of extracellular proteins from NT80 cells grown in the presence or absence of stearyl alcohol revealed that stearyl alcohol induced expression of several secretory proteins including lipase, haemolysin-coregulated protein and nucleoside diphosphate kinase. Expression of these secreted proteins was upregulated at the transcriptional level. Stearyl alcohol also induced the synthesis of polyhydroxyalkanoate. Secretory protein EliA was required for all these responses of NT80 cells to stearyl alcohol. Accordingly, the effects of stearyl alcohol were significantly reduced in the eliA deletion mutant cells of NT80 (ΔeliA). The remaining concentration of stearyl alcohol in the culture supernatant of the wild-type cells, but not that in the culture supernatant of the ΔeliA cells, clearly decreased during the course of growth. These observed phenotypes of the ΔeliA mutant were rescued by gene complementation. The results suggested that EliA is essential for these cells to respond to stearyl alcohol, and that it plays an important role in the recognition and assimilation of stearyl alcohol by NT80 cells.
authors.affiliation: Department of Applied Chemistry, Faculty of Science and Engineering, Chuo University, Bunkyo-ku, Tokyo, Japan.|Department of Life Science and Research Center for Life Science, College of Science, Rikkyo University, Toshima-ku, Tokyo, Japan.
authors.forename: Genki
authors.initials: G
authors.lastname: Akanuma
authors.affiliation: Department of Applied Chemistry, Faculty of Science and Engineering, Chuo University, Bunkyo-ku, Tokyo, Japan.
authors.forename: Rie
authors.initials: R
authors.lastname: Yoshizawa
authors.affiliation: Department of Applied Chemistry, Faculty of Science and Engineering, Chuo University, Bunkyo-ku, Tokyo, Japan.
authors.forename: Mari
authors.initials: M
authors.lastname: Nagakura
authors.affiliation: Genome Research Center, NODAI Research Institute, Tokyo University of Agriculture, Setagaya-ku, Tokyo, Japan.
authors.forename: Yuh
authors.initials: Y
authors.lastname: Shiwa
authors.affiliation: Department of Bioscience, Tokyo University of Agriculture, Setagaya-ku, Tokyo, Japan.
authors.forename: Satoru
authors.initials: S
authors.lastname: Watanabe
authors.affiliation: Genome Research Center, NODAI Research Institute, Tokyo University of Agriculture, Setagaya-ku, Tokyo, Japan.|Department of Bioscience, Tokyo University of Agriculture, Setagaya-ku, Tokyo, Japan.
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Yoshikawa
authors.affiliation: Department of Applied Chemistry and Biotechnology, Niihama National College of Technology, Niihama, Ehime, Japan.
authors.forename: Kazutoshi
authors.initials: K
authors.lastname: Ushio
authors.affiliation: Department of Applied Chemistry, Faculty of Science and Engineering, Chuo University, Bunkyo-ku, Tokyo, Japan.
authors.forename: Morio
authors.initials: M
authors.lastname: Ishizuka
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D018441:Biofilms / Q000254:growth & development; D003470:Culture Media / Q000737:chemistry; D005233:Fatty Alcohols / Q000378:metabolism*; D017353:Gene Deletion; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D006460:Hemolysin Proteins / Q000096:biosynthesis; D008049:Lipase / Q000096:biosynthesis; D046529:Microscopy, Electron, Transmission; D009701:Nucleoside-Diphosphate Kinase / Q000096:biosynthesis; D011091:Polyesters / Q000378:metabolism*; D054813:Polyhydroxyalkanoates / Q000096:biosynthesis; D043367:Ralstonia / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D005233:Fatty Alcohols; D006460:Hemolysin Proteins; D011091:Polyesters; D054813:Polyhydroxyalkanoates; C009316:stearyl alcohol; D009701:Nucleoside-Diphosphate Kinase; D008049:Lipase