title: Expressed Sequence Tags Analysis and Design of Simple Sequence Repeats Markers from a Full-Length cDNA Library in Perilla frutescens (L.).
abstract: Perilla frutescens is valuable as a medicinal plant as well as a natural medicine and functional food. However, comparative genomics analyses of P. frutescens are limited due to a lack of gene annotations and characterization. A full-length cDNA library from P. frutescens leaves was constructed to identify functional gene clusters and probable EST-SSR markers via analysis of 1,056 expressed sequence tags. Unigene assembly was performed using basic local alignment search tool (BLAST) homology searches and annotated Gene Ontology (GO). A total of 18 simple sequence repeats (SSRs) were designed as primer pairs. This study is the first to report comparative genomics and EST-SSR markers from P. frutescens will help gene discovery and provide an important source for functional genomics and molecular genetic research in this interesting medicinal plant.
authors.affiliation: Bioherb Research Institute, Kangwon National University, Chuncheon 200-701, Republic of Korea.
authors.forename: Eun Soo
authors.initials: ES
authors.lastname: Seong
authors.affiliation: Department of Bioconvergence Science and Technology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon 200-701, Republic of Korea.
authors.forename: Ji Hye
authors.initials: JH
authors.lastname: Yoo
authors.affiliation: Department of Bioconvergence Science and Technology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon 200-701, Republic of Korea.
authors.forename: Jae Hoo
authors.initials: JH
authors.lastname: Choi
authors.affiliation: Department of Bioconvergence Science and Technology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon 200-701, Republic of Korea.
authors.forename: Chang Heum
authors.initials: CH
authors.lastname: Kim
authors.affiliation: Department of Bioconvergence Science and Technology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon 200-701, Republic of Korea.
authors.forename: Mi Ran
authors.initials: MR
authors.lastname: Jeon
authors.affiliation: Department of Bioconvergence Science and Technology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon 200-701, Republic of Korea.
authors.forename: Byeong Ju
authors.initials: BJ
authors.lastname: Kang
authors.affiliation: Hwajin Cosmetics, Hongcheon 250-807, Republic of Korea.
authors.forename: Jae Geun
authors.initials: JG
authors.lastname: Lee
authors.affiliation: Department of Agricultural Life Sciences, Kangwon National University, Chuncheon 200-701, Republic of Korea.
authors.forename: Seon Kang
authors.initials: SK
authors.lastname: Choi
authors.affiliation: Department of Applied Bioscience, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Bimal Kumar
authors.initials: BK
authors.lastname: Ghimire
authors.affiliation: Department of Bioconvergence Science and Technology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon 200-701, Republic of Korea.
authors.forename: Chang Yeon
authors.initials: CY
authors.lastname: Yu