title: mRNA and protein expression of the angiogenesis-related genes EDIL3, AMOT and ECM1 in mesenchymal stem cells in psoriatic dermis.
abstract: BACKGROUND
  Dermal microvasculature expansion and angiogenesis are prominent in psoriasis. Our previous microarray study showed that the angiogenesis-related genes EDIL3 (epidermal growth factor-like repeats and discoidin I-like domains 3), AMOT (angiomotin) and ECM1 (extracellular matrix protein 1), had high expression levels in dermal mesenchymal stem cells (DMSCs) from psoriatic skin lesions.
  
  
  OBJECTIVE
  To investigate the mRNA and protein expressions of EDIL3, AMOT and ECM1 in DMSCs derived from psoriatic skin in order to better determine the molecular mechanisms of angiogenesis in the skin.
  
  
  METHODS
  DMSCs from 12 patients with psoriasis and 14 healthy controls (HCs) were cultured to passage 3, and identified by morphology, immunophenotype and multipotential differentiation. The mRNA and protein expressions of EDIL3, AMOT, and ECM1 in the DMSCs were determined using real-time reverse transcription PCR and western blotting.
  
  
  RESULTS
  DMSCs displayed spindle-like morphology and surface protein expression, and were able to differentiate into osteoblasts, chondrocytes and adipocytes. mRNA expression analysis showed that EDIL3, AMOT and ECM1 were expressed at 2.54-fold, 2.11-fold, and 1.90-fold higher levels, respectively, in psoriatic DMSCs compared with HC DMSCs (all P < 0.05). Protein analysis showed significantly (all P < 0.01) higher concentrations of EDIL3, AMOT and ECM1in the psoriasis group than in the HC group.
  
  
  CONCLUSIONS
  Our data demonstrate for the first time that expression of EDIL3, AMOT and ECM1 is altered in DMSCs in psoriasis, suggesting that EDIL3, AMOT and ECM1 are involved in the excessive angiogenesis and vasodilation observed in psoriasis.
authors.affiliation: Institute of Dermatology, Taiyuan City Central Hospital, Taiyuan, Shanxi, China.
authors.forename: X
authors.initials: X
authors.lastname: Niu
authors.affiliation: Institute of Dermatology, Taiyuan City Central Hospital, Taiyuan, Shanxi, China.
authors.forename: W
authors.initials: W
authors.lastname: Chang
authors.affiliation: Institute of Dermatology, Taiyuan City Central Hospital, Taiyuan, Shanxi, China.
authors.forename: R
authors.initials: R
authors.lastname: Liu
authors.affiliation: Institute of Dermatology, Taiyuan City Central Hospital, Taiyuan, Shanxi, China.
authors.forename: R
authors.initials: R
authors.lastname: Hou
authors.affiliation: Institute of Dermatology, Taiyuan City Central Hospital, Taiyuan, Shanxi, China.
authors.forename: J
authors.initials: J
authors.lastname: Li
authors.affiliation: Laboratory Animal Center, Shanxi Medical University, Taiyuan, Shanxi, China.
authors.forename: C
authors.initials: C
authors.lastname: Wang
authors.affiliation: Institute of Dermatology, Taiyuan City Central Hospital, Taiyuan, Shanxi, China.
authors.forename: X
authors.initials: X
authors.lastname: Li
authors.affiliation: Institute of Dermatology, Taiyuan City Central Hospital, Taiyuan, Shanxi, China.
authors.forename: K
authors.initials: K
authors.lastname: Zhang
mesh_terms: D000328:Adult; D000091082:Angiomotins; D002135:Calcium-Binding Proteins; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D016022:Case-Control Studies; D015815:Cell Adhesion Molecules; D002478:Cells, Cultured; D016326:Extracellular Matrix Proteins / Q000235:genetics / Q000378:metabolism*; D005260:Female; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D008840:Microfilament Proteins; D008875:Middle Aged; D009389:Neovascularization, Pathologic / Q000235:genetics; D011565:Psoriasis / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D012867:Skin / Q000098:blood supply / Q000378:metabolism*; D055815:Young Adult
chemical_list: C423985:AMOT protein, human; D000091082:Angiomotins; D002135:Calcium-Binding Proteins; D002352:Carrier Proteins; D015815:Cell Adhesion Molecules; C490607:ECM1 protein, human; C110183:EDIL3 protein, human; D016326:Extracellular Matrix Proteins; D036341:Intercellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D008840:Microfilament Proteins; D012333:RNA, Messenger