title: SpotCaliper: fast wavelet-based spot detection with accurate size estimation.
abstract: SpotCaliper is a novel wavelet-based image-analysis software providing a fast automatic detection scheme for circular patterns (spots), combined with the precise estimation of their size. It is implemented as an ImageJ plugin with a friendly user interface. The user is allowed to edit the results by modifying the measurements (in a semi-automated way), extract data for further analysis. The fine tuning of the detections includes the possibility of adjusting or removing the original detections, as well as adding further spots.
  
  
  
  The main advantage of the software is its ability to capture the size of spots in a fast and accurate way.
  
  
  
  http://bigwww.epfl.ch/algorithms/spotcaliper/
  
  
  
  zsuzsanna.puspoki@epfl.ch
  
  
  
  Supplementary data are available at Bioinformatics online.
authors.affiliation: Biomedical Imaging Group, École polytechnique fédérale de Lausanne (EPFL), Lausanne CH-1015, Switzerland.
authors.forename: Zsuzsanna
authors.initials: Z
authors.lastname: Püspöki
authors.affiliation: Biomedical Imaging Group, École polytechnique fédérale de Lausanne (EPFL), Lausanne CH-1015, Switzerland.
authors.forename: Daniel
authors.initials: D
authors.lastname: Sage
authors.affiliation: Biomedical Imaging Group, École polytechnique fédérale de Lausanne (EPFL), Lausanne CH-1015, Switzerland.
authors.forename: John Paul
authors.initials: JP
authors.lastname: Ward
authors.affiliation: Biomedical Imaging Group, École polytechnique fédérale de Lausanne (EPFL), Lausanne CH-1015, Switzerland.
authors.forename: Michael
authors.initials: M
authors.lastname: Unser
mesh_terms: D000465:Algorithms; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D012984:Software; D014584:User-Computer Interface