title: Antimicrobial Cellobiose Dehydrogenase-Chitosan Particles.
abstract: Increasing prevalence of chronic wounds and microbial infection constitute a severe health challenge. The situation is further complicated by emerging multidrug resistance making the treatment of infections increasingly difficult. Here, a novel antimicrobial system based on in situ release of hydrogen peroxide (H2O2) by cellobiose dehydrogenase (CDH) immobilized on chitosan (CTS) particles is described. Covalent immobilization using carbodiimide coupling lead to a higher amount of protein immobilized on CTS (104 μg CDH/mg CTS) when compared to noncovalent immobilization, which, however, showed highest recovery of CDH activity (0.01 U/mg CTS). The CDH-CTS in situ generated H2O2 completely inhibited growth of Escherichia coli and Staphylococcus aureus over a period of 24 h. This resilient antimicrobial system represents a novel strategy for preventing infection with potential application in counteracting microbial colonization of chronic wounds.
authors.affiliation: Institute of Environmental Biotechnology, BOKU-University of Natural Resources and Life Sciences , Vienna, Konrad Lorenz Straße 20, 3430 Tulln an der Donau, Austria.
authors.forename: Gregor
authors.initials: G
authors.lastname: Tegl
authors.affiliation: Institute of Environmental Biotechnology, BOKU-University of Natural Resources and Life Sciences , Vienna, Konrad Lorenz Straße 20, 3430 Tulln an der Donau, Austria.
authors.forename: Barbara
authors.initials: B
authors.lastname: Thallinger
authors.affiliation: Institute of Environmental Biotechnology, BOKU-University of Natural Resources and Life Sciences , Vienna, Konrad Lorenz Straße 20, 3430 Tulln an der Donau, Austria.
authors.forename: Bianca
authors.initials: B
authors.lastname: Beer
authors.affiliation: Department of Food Science and Technology, BOKU-University of Natural Resources and Life Sciences , Muthgasse 18, 1190 Vienna, Austria.
authors.forename: Christoph
authors.initials: C
authors.lastname: Sygmund
authors.affiliation: Department of Food Science and Technology, BOKU-University of Natural Resources and Life Sciences , Muthgasse 18, 1190 Vienna, Austria.
authors.forename: Roland
authors.initials: R
authors.lastname: Ludwig
authors.affiliation: Institute of Environmental Biotechnology, BOKU-University of Natural Resources and Life Sciences , Vienna, Konrad Lorenz Straße 20, 3430 Tulln an der Donau, Austria.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Rollett
authors.affiliation: Institute of Environmental Biotechnology, BOKU-University of Natural Resources and Life Sciences , Vienna, Konrad Lorenz Straße 20, 3430 Tulln an der Donau, Austria.|Botswana International University of Science and Technology , Private Bag 16, Palapye, Botswana.
authors.forename: Gibson S
authors.initials: GS
authors.lastname: Nyanhongo
authors.affiliation: Institute of Environmental Biotechnology, BOKU-University of Natural Resources and Life Sciences , Vienna, Konrad Lorenz Straße 20, 3430 Tulln an der Donau, Austria.|ACIB-Austrian Centre of Industrial Biotechnology , Konrad Lorenz Straße 20, 3430 Tulln, Austria.
authors.forename: Georg M
authors.initials: GM
authors.lastname: Guebitz
mesh_terms: D000327:Adsorption; D000890:Anti-Infective Agents / Q000494:pharmacology*; D002237:Carbohydrate Dehydrogenases / Q000378:metabolism*; D048271:Chitosan / Q000737:chemistry*; D003432:Cross-Linking Reagents / Q000494:pharmacology; D004795:Enzyme Stability / Q000187:drug effects; D004800:Enzymes, Immobilized / Q000378:metabolism; D004926:Escherichia coli / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D017550:Spectroscopy, Fourier Transform Infrared; D013211:Staphylococcus aureus / Q000187:drug effects; D013696:Temperature
chemical_list: D000890:Anti-Infective Agents; D003432:Cross-Linking Reagents; D004800:Enzymes, Immobilized; D048271:Chitosan; D002237:Carbohydrate Dehydrogenases; C019859:cellobiose-quinone oxidoreductase
keywords: cellobiose dehydrogenase; chronic wounds; enzyme; hydrogen peroxide; immobilization; infection