title: New tools for reconstruction and heterologous expression of natural product biosynthetic gene clusters.
abstract: Natural product scaffolds remain a major source and inspiration for human therapeutics. However, generation of a natural product in the post-genomic era often requires reconstruction of the corresponding biosynthetic gene cluster in a heterologous host. In the burgeoning fields of synthetic biology and metabolic engineering, a significant amount of efforts has been devoted to develop DNA assembly techniques with higher efficiency, fidelity, and modularity, and heterologous expression systems with higher productivity and yield. Here we describe recent advances in DNA assembly and host engineering and highlight their applications in natural product discovery and engineering.
authors.affiliation: State Key Laboratory of Biotherapy, West China Hospital, Sichuan University and Collaborative Innovation Center of Biotherapy, Chengdu, Sichuan, P. R. China and Department of Chemical and Biomolecular Engineering and Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA. zhao5@illinois.edu.
authors.forename: Yunzi
authors.initials: Y
authors.lastname: Luo
authors.affiliation: Department of Chemical and Biomolecular Engineering and Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA. zhao5@illinois.edu.
authors.forename: Behnam
authors.initials: B
authors.lastname: Enghiad
authors.affiliation: Department of Chemical and Biomolecular Engineering and Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA. zhao5@illinois.edu and Departments of Chemistry, Biochemistry and Bioengineering, Center for Biophysics and Computational Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
authors.forename: Huimin
authors.initials: H
authors.lastname: Zhao
mesh_terms: D001688:Biological Products / Q000737:chemistry / Q000378:metabolism* / Q000627:therapeutic use; D004247:DNA / Q000378:metabolism; D006801:Humans; D015394:Molecular Structure; D005810:Multigene Family
chemical_list: D001688:Biological Products; D004247:DNA