title: Seroepidemiological Survey of Canine Leishmania Infections from Peripheral Areas in Natal, Northeast Brazil.
abstract: OBJECTIVE
  Human visceral leishmaniasis is endemic in Natal, northeast of Brazil, where the domestic dog is an important parasite reservoir in the infectious cycle of Leishmania spp. In this study, was evaluated the antileishmanial IgG antibody and epidemiological factors related to canine visceral leishmaniasis (CVL).
  
  
  METHODS
  Sera samples obtained by venipuncture of 1,426 dogs living in areas of human visceral leishmaniasis occurrence were tested for detection of IgG anti-leishmania antibodies with Immunofluorescence Antibody Assay (IFA) and Enzyme Linked Immunosorbent Assay (ELISA). Chi-square (x(2)) and Odds Ratio (OR) were calculated. Differences were considered statistically significant at p≤0.05.
  
  
  RESULTS
  The overall seroprevalence was 10.30% (147/1,426); prevalence increased when the samples were found to be positive at least for one technique ([417/1,426] 29.3%). The high percentage of seroprevalence was observed in Nova República (15.35%), Nova Natal I (12.7%) and Lagoa Azul (11.4%) neighborhoods. In the Planalto, Soledade and Brasil Novo, the infection rates ranged from 7.5 at 8.06%. (p=0.00051). There was neither statistically significant difference between leishmanial infection nor clinical signs of disease (p=0.84; OR=1.0 [0.41; 2.3]), sex (p=0.78, OR=0.94 [0.66; 1.28]); and breed (p=0.92; OR=1.0 [0.65; 1.54]) were observed.
  
  
  CONCLUSIONS
  The RESULTS suggest that CVL is widely distributed in Natal, may be increasingly urbanizing and will spread through neighborhoods but not endemic, resulting in a serious public health problem, emphasizing the need for epidemiological studies to a greater understanding of the distribution of canine leishmaniasis in these specific areas and contribute proactively to the public health policies.
authors.affiliation: Centro de Controle de Zoonoses, Departamento de Vigilância à Saúde, Secretaria Municipal de Saúde. Natal/RN,Brazil ; Centro de Controle de Zoonoses, Departamento de Vigilância à Saúde, Secretaria Municipal de Saúde. Natal/RN,Brazil.
authors.forename: Isabelle Ribeiro
authors.initials: IR
authors.lastname: Barbosa
authors.affiliation: Centro de Controle de Zoonoses, Departamento de Vigilância à Saúde, Secretaria Municipal de Saúde. Natal/RN,Brazil.
authors.forename: Francisco Canindé
authors.initials: FC
authors.lastname: Carlota
authors.affiliation: Centro de Controle de Zoonoses, Departamento de Vigilância à Saúde, Secretaria Municipal de Saúde. Natal/RN,Brazil ; Centro de Controle de Zoonoses, Departamento de Vigilância à Saúde, Secretaria Municipal de Saúde. Natal/RN,Brazil.
authors.forename: Valter Ferreira
authors.initials: VF
authors.lastname: de Andrade-Neto
keywords: Canine visceral leishmaniasis; ELISA assay; Epidemiology; IFA assay.