title: A simple liquid extraction protocol for overcoming the ion suppression of triacylglycerols by phospholipids in liquid chromatography mass spectrometry studies.
abstract: It is well-known that triacylglycerol (TAG) ions are suppressed by phospholipid (PL) ions in regiospecific analysis of TAG by mass spectrometry (MS). Hence, it is essential to remove the PL during sample preparation prior to MS analysis. The present article proposes a cost-effective liquid-liquid extraction (LLE) method to remove PL from TAG in different kinds of biological samples by using methanol, hexane and water. High performance thin layer chromatography confirmed the lack of PL in krill oil and salmon liver samples, submitted to the proposed LLE protocol, and liquid chromatography tandem MS confirmed that the identified TAG ions were highly enhanced after implementing the LLE procedure.
authors.affiliation: National Institute of Nutrition and Seafood Research (NIFES), PO Box 2029 Nordnes, N-5817 Bergen, Norway. Electronic address: pedro.araujo@nifes.no.
authors.forename: Pedro
authors.initials: P
authors.lastname: Araujo
authors.affiliation: National Institute of Nutrition and Seafood Research (NIFES), PO Box 2029 Nordnes, N-5817 Bergen, Norway.
authors.forename: Ephrem
authors.initials: E
authors.lastname: Tilahun
authors.affiliation: National Institute of Nutrition and Seafood Research (NIFES), PO Box 2029 Nordnes, N-5817 Bergen, Norway.
authors.forename: Joar Fjørtoft
authors.initials: JF
authors.lastname: Breivik
authors.affiliation: National Institute of Nutrition and Seafood Research (NIFES), PO Box 2029 Nordnes, N-5817 Bergen, Norway.
authors.forename: Bashir M
authors.initials: BM
authors.lastname: Abdulkader
authors.affiliation: National Institute of Nutrition and Seafood Research (NIFES), PO Box 2029 Nordnes, N-5817 Bergen, Norway.
authors.forename: Livar
authors.initials: L
authors.lastname: Frøyland
authors.affiliation: Department of Chemistry, University of Bergen, N-5020 Bergen, Norway.
authors.forename: Yingxu
authors.initials: Y
authors.lastname: Zeng
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods; D033201:Euphausiacea; D005395:Fish Oils / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods; D010743:Phospholipids / Q000032:analysis*; D012474:Salmon; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014280:Triglycerides / Q000032:analysis*
chemical_list: D005395:Fish Oils; D010743:Phospholipids; D014280:Triglycerides
keywords: High perfomance thin layer chromatography; Liquid chromatography tandem mass spectrometric; Liquid–liquid extraction; Regiospecific analysis; Signal supression; Triacylglycerols