title: Single peak parameters technique for simultaneous measurements: Spectrophotometric sequential injection determination of Fe(II) and Fe(III).
abstract: Spectrophotometric sequential injection system (SI) is proposed to automate the method of simultaneous determination of Fe(II) and Fe(III) on the basis of parameters of a single peak. In the developed SI system, sample and mixture of reagents (1,10-phenanthroline and sulfosalicylic acid) are introduced into a vessel, where in an acid environment (pH≅3) appropriate compounds of Fe(II) and Fe(III) with 1,10-phenanthroline and sulfosalicylic acid are formed, respectively. Then, in turn, air, sample, EDTA and sample again, are introduced into a holding coil. After the flow reversal, a segment of air is removed from the system by an additional valve and as EDTA replaces sulfosalicylic acid forming a more stable colorless compound with Fe(III), a complex signal is registered. Measurements are performed at wavelength 530 nm. The absorbance measured at minimum of the negative peak and the area or the absorbance measured at maximum of the signal can be used as measures corresponding to Fe(II) and Fe(III) concentrations, respectively. The time of the peak registration is about 2 min. Two-component calibration has been applied to analysis. Fe(II) and Fe(III) can be determined within the concentration ranges of 0.04-4.00 and 0.1-5.00 mg L(-1), with precision less than 2.8% and 1.7% (RSD), respectively and accuracy better than 7% (RE). The detection limit is 0.04 and 0.09 mg L(-1) for Fe(II) and Fe(III), respectively. The method was applied to analysis of artesian water samples.
authors.affiliation: Faculty of Chemistry, Jagiellonian University in Krakow, Ingardena 3, 30-060 Krakow, Poland. Electronic address: j.kozak@uj.edu.pl.
authors.forename: J
authors.initials: J
authors.lastname: Kozak
authors.affiliation: Faculty of Chemistry, Jagiellonian University in Krakow, Ingardena 3, 30-060 Krakow, Poland.
authors.forename: J
authors.initials: J
authors.lastname: Paluch
authors.affiliation: Faculty of Chemistry, Jagiellonian University in Krakow, Ingardena 3, 30-060 Krakow, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Węgrzecka
authors.affiliation: Division of Petroleum Processing, Oil and Gas Institute - National Research Institute, Łukasiewicza 1, 31-429 Krakow, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Kozak
authors.affiliation: Faculty of Chemistry, Jagiellonian University in Krakow, Ingardena 3, 30-060 Krakow, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Wieczorek
authors.affiliation: Faculty of Chemistry, Jagiellonian University in Krakow, Ingardena 3, 30-060 Krakow, Poland.
authors.forename: J
authors.initials: J
authors.lastname: Kochana
authors.affiliation: Faculty of Chemistry, Jagiellonian University in Krakow, Ingardena 3, 30-060 Krakow, Poland.
authors.forename: P
authors.initials: P
authors.lastname: Kościelniak
keywords: Sequential injection analysis; Simultaneous determination of Fe(II) and Fe(III); Two-component analysis