title: [Rat intestine absorption kinetics study on cucurbitacin B-sodium deoxycholate/phospholipid mixed nanomicelles with in vitro everted gut sacs model].
abstract: To investigate the absorption kinetics of Cu B-SDC/PLC-MMs in rat different intestinal segments and compared with the absorption of Cu B suspension. The in vitro everted gut sacs model was established to study the absorption characteristics of Cu B-SDC/ PLC-MMs in rat duodenum, jejunum, ileum and colon, and the content of cucurbitacin B was detected by HPLC method, and the effects of concentrations on intestinal absorption were evaluated as well. The results showed that the absorption of Cu B-SDC/PLC-MMs was linearity at different intestine segment and different concentrations (R2 > 0.9), which was consistent with zero order rate process. The Ka of different intestine segments showed a concentration-dependent increasing along with the raised concentration of Cu B-SDC/ PLC-MMs, indicating that it was likely to be a mechanism of passive absorption. The best absorption site of Cu B-SDC/PLC-MMs was ileum, and its absorptions in different intestinal segments were superior to cucurbitacin B suspension. SDC/PLC-MMs could significantly enhance the intestinal absorption of cucurbitacin B, and the study of intestinal absorption kinetics of Cu B-SDC/PLC-MMs had gave a support to its further reasonable solidfication.
authors.forename: Ling
authors.initials: L
authors.lastname: Cheng
authors.forename: Bao-de
authors.initials: BD
authors.lastname: Shen
authors.forename: Juan-juan
authors.initials: JJ
authors.lastname: Li
authors.forename: Ling
authors.initials: L
authors.lastname: Qiu
authors.forename: Gang
authors.initials: G
authors.lastname: Shen
authors.forename: Li-hong
authors.initials: LH
authors.lastname: Zhang
authors.forename: Jin
authors.initials: J
authors.lastname: Han
authors.forename: Hai-long
authors.initials: HL
authors.lastname: Yuan
mesh_terms: D000818:Animals; D003840:Deoxycholic Acid / Q000008:administration & dosage*; D005260:Female; D007408:Intestinal Absorption; D007700:Kinetics; D008297:Male; D008823:Micelles; D053758:Nanoparticles; D010743:Phospholipids / Q000008:administration & dosage*; D051381:Rats; D017208:Rats, Wistar; D014315:Triterpenes / Q000008:administration & dosage / Q000493:pharmacokinetics*
chemical_list: D008823:Micelles; D010743:Phospholipids; D014315:Triterpenes; D003840:Deoxycholic Acid; C041246:cucurbitacin B