title: Anti-tumor activity evaluation of novel chrysin-organotin compound in MCF-7 cells.
abstract: Chrysin (5,7-dihydroxylflavone, Chry) is a natural flavonoid extracted from plants and propolis. In this work, a novel chrysin-organotin (Chry-Sn) compound with enhanced anticancer activities was synthesized by the reaction of chrysin and triphenyltin chloride, and its potential anticancer effects against cancer cells were measured using various methods. Sulforhodamine B (SRB) results showed that chrysin and Chry-Sn had significant inhibition effects on the proliferation of MCF-7, A549 and HeLa human cancer cell lines in a dose- and time- dependent manner. These results suggested that Chry-Sn possessed enhanced anticancer effects. Hoechst 33258 staining and acridine orange staining results showed apoptosis and nuclei fragments significantly increased after being treated with chrysin and Chry-Sn respectively. Moreover, chrysin and Chry-Sn significantly increased ROS levels in MCF-7 cells. Western blot results showed that chrysin and Chry-Sn activated caspase 3 and induced autophagy by increasing LC3-II level. All results showed collectively that Chry-Sn could be a more promising drug than chrysin in anticancer treatment.
authors.affiliation: School of Life Science, Liaocheng University, Liaocheng 252059, PR China. Electronic address: hongzhuanxuan@163.com.
authors.forename: Hong-Zhuan
authors.initials: HZ
authors.lastname: Xuan
authors.affiliation: School of Chemical & Chemical Engineering, Liaocheng University, Liaocheng 252059, PR China.
authors.forename: Jun-Hong
authors.initials: JH
authors.lastname: Zhang
authors.affiliation: School of Life Science, Liaocheng University, Liaocheng 252059, PR China.
authors.forename: Yue-Hua
authors.initials: YH
authors.lastname: Wang
authors.affiliation: School of Life Science, Liaocheng University, Liaocheng 252059, PR China.
authors.forename: Chong-Luo
authors.initials: CL
authors.lastname: Fu
authors.affiliation: School of Life Science, Liaocheng University, Liaocheng 252059, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D001343:Autophagy / Q000187:drug effects; D053148:Caspase 3 / Q000378:metabolism; D045744:Cell Line, Tumor; D005419:Flavonoids / Q000737:chemistry* / Q000494:pharmacology*; D006367:HeLa Cells; D006801:Humans; D061986:MCF-7 Cells; D009947:Organotin Compounds / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D005419:Flavonoids; D009947:Organotin Compounds; C043561:chrysin; D053148:Caspase 3
keywords: Apoptosis; Autophagy; Chrysin; Chrysin–organotin; MCF-7 cells