title: Plant Cell Division Analyzed by Transient Agrobacterium-Mediated Transformation of Tobacco BY-2 Cells.
abstract: The continuing analysis of plant cell division will require additional protein localization studies. This is greatly aided by GFP-technology, but plant transformation and the maintenance of transgenic lines can present a significant technical bottleneck. In this chapter I describe a method for the Agrobacterium-mediated genetic transformation of tobacco BY-2 cells. The method allows for the microscopic analysis of fluorescence-tagged proteins in dividing cells in within 2 days after starting a coculture. This transient transformation procedure requires only standard laboratory equipment. It is hoped that this rapid method would aid researchers conducting live-cell localization studies in plant mitosis and cytokinesis.
authors.affiliation: Botany Department, School of Biology and Chemistry, Osnabrück University, Postfach 44 69, Osnabrück, 49069, Germany. Henrik.buschmann@biologie.uni-osnabrueck.de.
authors.forename: Henrik
authors.initials: H
authors.lastname: Buschmann
mesh_terms: D060054:Agrobacterium / Q000235:genetics*; D002455:Cell Division; D018920:Coculture Techniques / Q000379:methods; D048749:Cytokinesis; D049452:Green Fluorescent Proteins / Q000032:analysis; D008856:Microscopy, Fluorescence / Q000379:methods; D008938:Mitosis; D059828:Plant Cells / Q000378:metabolism / Q000382:microbiology; D030821:Plants, Genetically Modified / Q000166:cytology* / Q000235:genetics* / Q000382:microbiology; D014026:Nicotiana / Q000166:cytology* / Q000235:genetics* / Q000382:microbiology; D014170:Transformation, Genetic
chemical_list: D049452:Green Fluorescent Proteins
keywords: Agrobacterium; Cell division; Cytokinesis; F-actin; Green fluorescent protein; Tobacco BY-2; Transient transformation