title: Laboratory Evaluation of a Dual-Path Platform Assay for Rapid Point-of-Care HIV and Syphilis Testing.
abstract: We assessed the laboratory performance of the Chembio dual-path platform HIV-syphilis rapid immunodiagnostic test and electronic reader for detection of HIV and Treponema pallidum antibodies in 450 previously characterized serum specimens. For visual or electronic reader HIV antibody detection, the sensitivity was 100% and the specificity was 98.7%. For visual T. pallidum antibody detection, the test sensitivity was 94.7% and the specificity was 100.0%; with the electronic reader, the sensitivity was 94.7% and the specificity was 99.7%.
authors.affiliation: Laboratory of Sexual Health, Universidad Peruana Cayetano Heredia, Lima, Peru Dirección de Investigación, Socios en Salud Sucursal del Perú, Lima, Peru srleons@gmail.com.
authors.forename: S R
authors.initials: SR
authors.lastname: Leon
authors.affiliation: Laboratory of Sexual Health, Universidad Peruana Cayetano Heredia, Lima, Peru.
authors.forename: L B
authors.initials: LB
authors.lastname: Ramos
authors.affiliation: Laboratory of Sexual Health, Universidad Peruana Cayetano Heredia, Lima, Peru.
authors.forename: S K
authors.initials: SK
authors.lastname: Vargas
authors.affiliation: David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California, USA.
authors.forename: N
authors.identifier: http://orcid.org/0000-0002-3667-9719
authors.initials: N
authors.lastname: Kojima
authors.affiliation: Laboratory of Sexual Health, Universidad Peruana Cayetano Heredia, Lima, Peru Department of Clinical Pathology, National Hospital Daniel A. Carrion, Callao, Peru.
authors.forename: D G
authors.initials: DG
authors.lastname: Perez
authors.affiliation: Laboratory of Sexual Health, Universidad Peruana Cayetano Heredia, Lima, Peru.
authors.forename: C F
authors.initials: CF
authors.lastname: Caceres
authors.affiliation: Division of Infectious Diseases, Department of Medicine, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California, USA.
authors.forename: J D
authors.initials: JD
authors.lastname: Klausner
mesh_terms: D000942:Antigens, Bacterial / Q000276:immunology; D060085:Coinfection; D015483:HIV Antibodies / Q000276:immunology; D016655:HIV Core Protein p24 / Q000276:immunology; D015658:HIV Infections / Q000175:diagnosis*; D006801:Humans; D007159:Immunologic Tests / Q000379:methods / Q000592:standards; D019095:Point-of-Care Systems; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013587:Syphilis / Q000175:diagnosis*
chemical_list: D000942:Antigens, Bacterial; D015483:HIV Antibodies; D016655:HIV Core Protein p24