title: Variants and haplotypes in Flap endonuclease 1 and risk of gallbladder cancer and gallstones: a population-based study in China.
abstract: The role of FEN1 genetic variants on gallstone and gallbladder cancer susceptibility is unknown. FEN1 SNPs were genotyped using the polymerase chain reaction-restriction fragment length polymorphism method in blood samples from 341 gallbladder cancer patients and 339 healthy controls. The distribution of FEN1-69G > A genotypes among controls (AA, 20.6%; GA, 47.2% and GG 32.2%) was significantly different from that among gallbladder cancer cases (AA, 11.1%; GA, 48.1% and GG, 40.8%), significantly increased association with gallbladder cancer was observed for subjects with both the FEN1-69G > A GA (OR = 1.73, 95% CI = 1.01-2.63) and the FEN1-69G > A GG (OR = 2.29, 95% CI = 1.31-3.9). The distribution of FEN1 -4150T genotypes among controls (TT, 21.8%;GT, 49.3% and GG 28.9%) was significantly different from that among gallbladder cancer cases (TT, 12.9%; GT, 48.4% and GG 38.7%), significantly increased association with gallbladder cancer was observed for subjects with both the FEN1-4150T GT(OR = 1.93, 95% CI = 1.04-2.91) and the FEN1-4150T GG(OR = 2.56, 95% CI = 1.37-5.39). A significant trend towards increased association with gallbladder cancer was observed with potentially higher-risk FEN1-69G > A genotypes (P < 0.001, χ2 trend test) and FEN14150G > T (P < 0.001, χ2 trend test) in gallstone presence but not in gallstone absence (P = 0.81, P = 0.89, respectively). In conclusion, this study revealed firstly that FEN1 polymorphisms and haplotypes are associated with gallbladder cancer risk.
authors.affiliation: Department of General Surgery, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510080, China.|Department of General Surgery and Transplantation Surgery, University Hospital Duisburg-Essen, D-45122, Germany.
authors.forename: Xingyuan
authors.initials: X
authors.lastname: Jiao
authors.affiliation: Department of Biostatistics, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510080, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of General Surgery, The First Affiliated Hospital, Xian Medical College, Xian 710061, China.
authors.forename: Liansuo
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Department of General Surgery, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510080, China.
authors.forename: Jinyun
authors.initials: J
authors.lastname: He
authors.affiliation: Department of General Surgery, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510080, China.
authors.forename: Chonghua
authors.initials: C
authors.lastname: Yang
authors.affiliation: Department of General Surgery, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510080, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Department of General Surgery, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510080, China.
authors.forename: Ronglin
authors.initials: R
authors.lastname: Hu
authors.affiliation: Deparment of Pathology, Sun Yat-Sen University School of Medicine, Guangzhou 510080, China.
authors.forename: Canqiao
authors.initials: C
authors.lastname: Luo
authors.affiliation: Department of Molecular Biology, Sun Yat-Sen University School of Pharmacy, Guangzhou 510080, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Du
authors.affiliation: Department of General Surgery and Transplantation Surgery, University Hospital Duisburg-Essen, D-45122, Germany.
authors.forename: Jian
authors.initials: J
authors.lastname: Fu
authors.affiliation: Department of Hepatobiliary Surgery, The First Affiliated Hospital, Xian Jiaotong University, Xian 710061, China.
authors.forename: Jinsen
authors.initials: J
authors.lastname: Shi
authors.affiliation: Department of General Surgery, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510080, China.
authors.forename: Rui
authors.initials: R
authors.lastname: He
authors.affiliation: Department of General Surgery, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510080, China.
authors.forename: Dongming
authors.initials: D
authors.lastname: Li
authors.affiliation: Department of Anatomy, Shenzhen University School of Medicine, Shenzhen 518060, China.
authors.forename: Wang
authors.initials: W
authors.lastname: Jun
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D044466:Asian People / Q000235:genetics; D002681:China; D005260:Female; D045585:Flap Endonucleases / Q000235:genetics*; D005706:Gallbladder Neoplasms / Q000201:enzymology / Q000208:ethnology / Q000235:genetics*; D042882:Gallstones / Q000201:enzymology / Q000208:ethnology / Q000235:genetics*; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease / Q000208:ethnology / Q000235:genetics*; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D015810:Linkage Disequilibrium; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D011159:Population Surveillance / Q000379:methods; D012307:Risk Factors
chemical_list: D045585:Flap Endonucleases