title: NdhV Is a Subunit of NADPH Dehydrogenase Essential for Cyclic Electron Transport in Synechocystis sp. Strain PCC 6803.
abstract: Two mutants sensitive to heat stress for growth and impaired in NADPH dehydrogenase (NDH-1)-dependent cyclic electron transport around photosystem I (NDH-CET) were isolated from the cyanobacterium Synechocystis sp. strain PCC 6803 transformed with a transposon-bearing library. Both mutants had a tag in the same sll0272 gene, encoding a protein highly homologous to NdhV identified in Arabidopsis (Arabidopsis thaliana). Deletion of the sll0272 gene (ndhV) did not influence the assembly of NDH-1 complexes and the activities of CO2 uptake and respiration but reduced the activity of NDH-CET. NdhV interacted with NdhS, a ferredoxin-binding subunit of cyanobacterial NDH-1 complex. Deletion of NdhS completely abolished NdhV, but deletion of NdhV had no effect on the amount of NdhS. Reduction of NDH-CET activity was more significant in ΔndhS than in ΔndhV. We therefore propose that NdhV cooperates with NdhS to accept electrons from reduced ferredoxin.
authors.affiliation: College of Life and Environment Sciences, Shanghai Normal University, Shanghai 200234, China.
authors.forename: Fudan
authors.identifier: http://orcid.org/0000-0002-8882-5968
authors.initials: F
authors.lastname: Gao
authors.affiliation: College of Life and Environment Sciences, Shanghai Normal University, Shanghai 200234, China.
authors.forename: Jiaohong
authors.initials: J
authors.lastname: Zhao
authors.affiliation: College of Life and Environment Sciences, Shanghai Normal University, Shanghai 200234, China.
authors.forename: Xiaozhuo
authors.initials: X
authors.lastname: Wang
authors.affiliation: College of Life and Environment Sciences, Shanghai Normal University, Shanghai 200234, China.
authors.forename: Shen
authors.initials: S
authors.lastname: Qin
authors.affiliation: College of Life and Environment Sciences, Shanghai Normal University, Shanghai 200234, China.
authors.forename: Lanzhen
authors.initials: L
authors.lastname: Wei
authors.affiliation: College of Life and Environment Sciences, Shanghai Normal University, Shanghai 200234, China wma@shnu.edu.cn.
authors.forename: Weimin
authors.identifier: http://orcid.org/0000-0003-4964-415X
authors.initials: W
authors.lastname: Ma
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D002245:Carbon Dioxide / Q000378:metabolism; D004579:Electron Transport; D005288:Ferredoxins / Q000378:metabolism; D008958:Models, Molecular; D009154:Mutation; D009252:NADPH Dehydrogenase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D045331:Photosystem I Protein Complex / Q000378:metabolism; D011485:Protein Binding; D000072417:Protein Domains; D021122:Protein Subunits; D017384:Sequence Deletion; D046939:Synechocystis / Q000201:enzymology* / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D005288:Ferredoxins; D045331:Photosystem I Protein Complex; D021122:Protein Subunits; D002245:Carbon Dioxide; D009252:NADPH Dehydrogenase