title: Physiological, morphological and biochemical studies of glyphosate tolerance in Mexican Cologania (Cologania broussonetii (Balb.) DC.).
abstract: In recent years, glyphosate-tolerant legumes have been used as cover crops for weed management in tropical areas of Mexico. Mexican cologania (Cologania broussonetii (Balb.) DC.) is an innate glyphosate-tolerant legume with a potential as a cover crop in temperate areas of the country. In this work, glyphosate tolerance was characterized in two Mexican cologania (a treated (T) and an untreated (UT)) populations as being representatives of the species, compared in turn to a glyphosate-susceptible hairy fleabane (S) (Conyza bonariensis (L.) Cronq.) population. Experiments revealed that T and UT Mexican cologania populations had a higher tolerance index (TI), and a lower shikimic acid accumulation and foliar retention than the hairy fleabane S population. Absorption and translocation, leaf morphology and metabolism studies were only carried out in the Mexican cologania T population and the hairy fleabane S population. The latter absorbed 37% more (14)C-glyphosate compared to the Mexican cologania T at 96 h after treatment (HAT). Mexican cologania T translocated less herbicide from the treated leaf to the remainder of the plant than hairy fleabane S. The Mexican cologania T presented a greater epicuticular wax coverage percentage than the hairy fleabane S. This morphological characteristic contributed to the low glyphosate absorption observed in the Mexican cologania. In addition, the Mexican cologania T metabolized glyphosate mainly into AMPA, formaldehyde and sarcosine. These results indicate that the high glyphosate tolerance observed in Mexican cologania is mainly due to the poor penetration and translocation of glyphosate into the active site, and the high glyphosate degradation into non-toxic substances.
authors.affiliation: Department of Agricultural Chemistry and Edaphology, University of Cordoba, E-14071, Cordoba, Spain. Electronic address: g12alalr@uco.es.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Alcántara de la Cruz
authors.affiliation: Institute for Sustainable Agriculture, Spanish National Research Council (IAS-CSIC), E-14004, Córdoba, Spain.
authors.forename: Francisco
authors.initials: F
authors.lastname: Barro
authors.affiliation: Department of Agricultural Parasitology, Chapingo Autonomous University, E-56230, Texcoco, Mexico.
authors.forename: José Alfredo
authors.initials: JA
authors.lastname: Domínguez-Valenzuela
authors.affiliation: Department of Agricultural Chemistry and Edaphology, University of Cordoba, E-14071, Cordoba, Spain.
authors.forename: Rafael
authors.initials: R
authors.lastname: De Prado
mesh_terms: D001692:Biological Transport; D035401:Conyza / Q000033:anatomy & histology / Q000187:drug effects / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D007887:Fabaceae / Q000033:anatomy & histology / Q000187:drug effects / Q000502:physiology*; D005998:Glycine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D053209:Herbicide Resistance; D006540:Herbicides / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D018515:Plant Leaves / Q000033:anatomy & histology / Q000187:drug effects / Q000502:physiology; D012765:Shikimic Acid / Q000378:metabolism; D064201:Trichomes; D000097797:Glyphosate
chemical_list: D005998:Glycine; D006540:Herbicides; D012765:Shikimic Acid; D000097797:Glyphosate
keywords: Absorption/translocation; Cover crop; Glyphosate metabolism; Glyphosate tolerance; Morphological characteristics; Shikimic acid