title: Fabrication of multi-biofunctional gelatin-based electrospun fibrous scaffolds for enhancement of osteogenesis of mesenchymal stem cells.
abstract: Biofunctional scaffolds that support the adhesion, proliferation, and osteo-differentiation of mesenchymal stem cells (MSCs) are critical for bone tissue engineering. In this study, a simple in situ UV-crosslinking strategy was utilized to fabricate gelatin electrospun fibrous (GEF) scaffolds with multiple biosignals, including cell adhesive Arg-Gly-Asp (RGD) peptide, osteo-conductive hydroxyapatite (HAp) nanoparticles, and osteo-inductive bone morphogenic protein-2 (BMP-2). The adhesion and proliferation of MSCs on the GEF scaffolds were improved by the incorporation of RGD. Meanwhile, the incorporation of HAp and BMP-2 enhanced osteo-differentiation of MSCs. The three incorporated bio-factors exert a synergistic effect on osteogenesis of MSCs in the GEF scaffolds. This strategy of incorporating multiple biomolecules could be used to fabricate crosslinked electrospun scaffolds of natural polymers for tissue-engineering applications.
authors.affiliation: Department of Chemical Engineering, National Taiwan University, No. 1, Roosevelt Rd., Sec. 4, Taipei 106, Taiwan.
authors.forename: Wei-Han
authors.initials: WH
authors.lastname: Lin
authors.affiliation: Department of Chemical Engineering, National Taiwan University, No. 1, Roosevelt Rd., Sec. 4, Taipei 106, Taiwan.
authors.forename: Jiashing
authors.initials: J
authors.lastname: Yu
authors.affiliation: Tissue Regeneration Materials Unit, International Center for Materials Nanoarchitectonics (MANA), National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan.
authors.forename: Guoping
authors.initials: G
authors.lastname: Chen
authors.affiliation: Department of Chemical Engineering, National Taiwan University, No. 1, Roosevelt Rd., Sec. 4, Taipei 106, Taiwan. Electronic address: weibortsai@ntu.edu.tw.
authors.forename: Wei-Bor
authors.initials: WB
authors.lastname: Tsai
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D002460:Cell Line; D017886:Durapatite / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D055664:Electrochemical Techniques / Q000379:methods; D005780:Gelatin / Q000737:chemistry* / Q000378:metabolism / Q000528:radiation effects; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D053758:Nanoparticles / Q000737:chemistry / Q000378:metabolism; D009842:Oligopeptides / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D010012:Osteogenesis / Q000187:drug effects / Q000502:physiology*; D015203:Reproducibility of Results; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D014466:Ultraviolet Rays
chemical_list: D055396:Bone Morphogenetic Protein 2; D009842:Oligopeptides; C047981:arginyl-glycyl-aspartic acid; D005780:Gelatin; D017886:Durapatite; D000469:Alkaline Phosphatase; D002118:Calcium
keywords: Bone morphogenetic protein-2; Electrospun fibers; Gelatin; Hydroxyapatite; Osteogenesis; RGD