title: Purification and characterization of a chloride ion-dependent α-glucosidase from the midgut gland of Japanese scallop (Patinopecten yessoensis).
abstract: Marine glycoside hydrolases hold enormous potential due to their habitat-related characteristics such as salt tolerance, barophilicity, and cold tolerance. We purified an α-glucosidase (PYG) from the midgut gland of the Japanese scallop (Patinopecten yessoensis) and found that this enzyme has unique characteristics. The use of acarbose affinity chromatography during the purification was particularly effective, increasing the specific activity 570-fold. PYG is an interesting chloride ion-dependent enzyme. Chloride ion causes distinctive changes in its enzymatic properties, increasing its hydrolysis rate, changing the pH profile of its enzyme activity, shifting the range of its pH stability to the alkaline region, and raising its optimal temperature from 37 to 55 °C. Furthermore, chloride ion altered PYG's substrate specificity. PYG exhibited the highest Vmax/Km value toward maltooctaose in the absence of chloride ion and toward maltotriose in the presence of chloride ion.
authors.affiliation: a Research Faculty of Agriculture , Hokkaido University , Sapporo , Japan.
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Masuda
authors.affiliation: a Research Faculty of Agriculture , Hokkaido University , Sapporo , Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Okuyama
authors.affiliation: a Research Faculty of Agriculture , Hokkaido University , Sapporo , Japan.
authors.forename: Takahisa
authors.initials: T
authors.lastname: Iizuka
authors.affiliation: a Research Faculty of Agriculture , Hokkaido University , Sapporo , Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Nakai
authors.affiliation: a Research Faculty of Agriculture , Hokkaido University , Sapporo , Japan.
authors.forename: Wataru
authors.initials: W
authors.lastname: Saburi
authors.affiliation: a Research Faculty of Agriculture , Hokkaido University , Sapporo , Japan.
authors.forename: Taro
authors.initials: T
authors.lastname: Fukukawa
authors.affiliation: a Research Faculty of Agriculture , Hokkaido University , Sapporo , Japan.
authors.forename: Janjira
authors.initials: J
authors.lastname: Maneesan
authors.affiliation: a Research Faculty of Agriculture , Hokkaido University , Sapporo , Japan.
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Tagami
authors.affiliation: b Aomori Industrial Research Center , Aomori , Japan.
authors.forename: Tetsushi
authors.initials: T
authors.lastname: Naraoka
authors.affiliation: a Research Faculty of Agriculture , Hokkaido University , Sapporo , Japan.
authors.forename: Haruhide
authors.initials: H
authors.lastname: Mori
authors.affiliation: a Research Faculty of Agriculture , Hokkaido University , Sapporo , Japan.
authors.forename: Atsuo
authors.initials: A
authors.lastname: Kimura
mesh_terms: D000818:Animals; D002712:Chlorides / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007700:Kinetics; D049897:Pectinidae; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013379:Substrate Specificity; D013696:Temperature; D000520:alpha-Glucosidases / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D002712:Chlorides; D000520:alpha-Glucosidases
keywords: chloride ion dependent; scallop α-glucosidase; substrate specificity