title: Effect of adjunctive application of epigallocatechin-3-gallate and ethanol-wet bonding on adhesive-dentin bonds.
abstract: OBJECTIVE
  To determine the effect of the combined use of epigallocatechin-3-gallate (EGCG) and ethanol-wet bonding (EWB) on resin-dentin bonds.
  
  
  METHODS
  Sixty molars were sectioned, polished, and randomly divided into six groups (n=10) according to the following pretreatments: group 1, water-wet bonding (WWB); group 2, WWB with 0.02% (w/v) EGCG; group 3, WWB with 0.1% EGCG; group 4, EWB; group 5, EWB with 0.02% EGCG; and group 6, EWB with 0.1% EGCG. An etch-and-rinse adhesive was then used, followed by the resin composites building. The microtensile bond strength (MTBS), failure modes and interfacial nanoleakage were separately determined after 24h water storage or 10,000 runs of thermocycling.
  
  
  RESULTS
  Both pretreatment method (P<0.05) and thermocycling (P<0.05) significantly influenced bond strength and nanoleakage. Irrespective of thermocycling, the 0.02% EGCG/ethanol (group 5) pretreated adhesive-dentin interfaces showed higher MTBS than the control group (P<0.05). Nanoleakage expression of all groups increased after thermocycling (P<0.05) except group 5. Adhesive failure was the main fracture pattern in all groups.
  
  
  CONCLUSIONS
  This study showed that pretreatment with 0.02% EGCG/ethanol solutions can effectively improve immediate bond strength and bond stability of etch-and-rinse adhesives on dentin.
  
  
  CONCLUSIONS
  The adjunctive application of EGCG and EWB provides a new strategy for dentists to obtain the desired bond effectiveness during adhesive restoration in clinical practice.
authors.affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedical Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, China.
authors.forename: Hongye
authors.initials: H
authors.lastname: Yang
authors.affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedical Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, China.
authors.forename: Jingmei
authors.initials: J
authors.lastname: Guo
authors.affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedical Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, China.
authors.forename: Donglai
authors.initials: D
authors.lastname: Deng
authors.affiliation: College of Stomatology, Guangxi Medical University, Nanning, China.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Chen
authors.affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedical Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, China. Electronic address: huangcui@whu.edu.cn.
authors.forename: Cui
authors.initials: C
authors.lastname: Huang
mesh_terms: D000134:Acid Etching, Dental / Q000379:methods; D000269:Adhesives / Q000737:chemistry*; D002392:Catechin / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000737:chemistry; D003188:Composite Resins / Q000737:chemistry; D001840:Dental Bonding / Q000379:methods*; D003804:Dentin / Q000737:chemistry / Q000187:drug effects; D017220:Dentin-Bonding Agents / Q000737:chemistry*; D000431:Ethanol / Q000008:administration & dosage / Q000737:chemistry*; D006801:Humans; D008689:Methacrylates / Q000737:chemistry; D008964:Molar, Third; D011897:Random Allocation; D014867:Water / Q000737:chemistry
chemical_list: D000269:Adhesives; D003188:Composite Resins; D017220:Dentin-Bonding Agents; D008689:Methacrylates; D014867:Water; D000431:Ethanol; D002392:Catechin; C045651:epigallocatechin gallate
keywords: Adhesive; Dentin; Epigallocatechin-3-gallate; Ethanol–wet bonding