title: [Cloning and expression analysis of transcription factor gene DoWRKY1 in Dendrobium officinale].
abstract: WRKY transcription factors are novel transcriptional regulatory factors, which play an important role in regulating plant development, metabolism and other physiological processes. In this study, a new Dendrobium officinale WRKY transcription factor, designated as DoWRKY1 was cloned by using RT-PCR and RACE (GenBank Accession No. KF953910). Bioinformatic analysis demonstrated that, the full-length cDNA of DoWRKY1 was 1,704 bp. And DoWRKY1 contained a 1,629 bp open reading frame (ORF) that encoding a peptide of 542 amino acid residues. The putative DoWRKY1 protein contained two conserved WRKY domains and it belonged to the group I WRKY family protein. Yeast one-hybrid experiment showed that DoWRKY1 had transcriptional activation ability in yeast, and it could activate the expression of downstream report genes (His3 and Ade2). Semi-quantitative RT-PCR experiment showed that DoWRKY1 expressed in roots, stems, leaves and protocorm-like bodies. Real-time qRT-PCR proved that DoWRKY1 could be induced by methyl jasmonate (MeJA) and chitosan (Chitosan), and the expression level of this gene can reach the expression peak at 2 h and 1 h, respectively. These results are useful for further determination of the regulation function of this gene in secondary metabolism of D. officinale.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhao
authors.forename: Shi-wei
authors.initials: SW
authors.lastname: Sun
authors.forename: Can-can
authors.initials: CC
authors.lastname: Meng
authors.forename: Qing
authors.initials: Q
authors.lastname: Jin
authors.forename: Hong-hong
authors.initials: HH
authors.lastname: Fan
authors.forename: Yi
authors.initials: Y
authors.lastname: Lin
authors.forename: Yong-ping
authors.initials: YP
authors.lastname: Cai
mesh_terms: D003001:Cloning, Molecular; D031667:Dendrobium / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D010940:Plant Proteins / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D010940:Plant Proteins; D014157:Transcription Factors