title: Establishment of drug delivery system nanocapsulated with an antioxidant (+)-catechin hydrate and sodium meta borate chelator against sodium fluoride induced oxidative stress in rats.
abstract: Oxidative stress a major cause of fluoride induced toxicity and mitochondrial impairment in common in experimental rats during chronic exposure of fluoride. Attempts have been made in the present experiment to diminish oxidative damage, combined therapy with (+)-catechin hydrate (an antioxidant) and sodium meta borate (chelator) were used. Fluoride intoxication in rats was performed by using 13mg/kg NaF and both antioxidant CH and chelator SMB were used at a concentration of 8.98μM/kg body weight. Mixture of CH and SMB in free or in PLGA nanocapsule encapsulated form were prepared. The efficacies of those formulations were tested in combating free radical mediated oxidative insult produced by sodium fluoride (NaF). The amalgamated therapy used in this experiment was shown to reduce fluoride levels in liver, brain and kidney from 9.5, 5.5, 6.3μg/g to 4.6, 2, 2.6μg/g, respectively. Our result indicated that the combined chelator and antioxidant therapy in nanocapsulated drug delivery system could provide a projection in combating fluoride induced mitochondrial impairment in rat model.
authors.affiliation: Department of Biotechnology, Oriental Institute of Science and Technology (Vidyasagar University), Dewandighi, Burdwan 02, India. Electronic address: arpita.pari@gmail.com.
authors.forename: Arpita
authors.initials: A
authors.lastname: Samanta
authors.affiliation: Central Institute of Freshwater Aquaculture, Rahara, India. Electronic address: s.bt.chanda@gmail.com.
authors.forename: Soumen
authors.initials: S
authors.lastname: Chanda
authors.affiliation: Department of Biotechnology, Oriental Institute of Science and Technology (Vidyasagar University), Dewandighi, Burdwan 02, India. Electronic address: bidyut2006@gmail.com.
authors.forename: Bidyut
authors.initials: B
authors.lastname: Bandyopadhyay
authors.affiliation: Drug Development Diagnostics and Biotechnology, CSIR-Indian Institute of Chemical Biology, 4, Raja S.C. Mullick Road, Jadavpur, Kolkata 700032, India. Electronic address: dasnirmalendu@hotmail.com.
authors.forename: Nirmalendu
authors.initials: N
authors.lastname: Das
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001881:Borates / Q000494:pharmacology*; D001921:Brain / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D002392:Catechin / Q000737:chemistry / Q000494:pharmacology*; D002614:Chelating Agents / Q000494:pharmacology*; D003094:Collagen / Q000378:metabolism; D016503:Drug Delivery Systems; D005260:Female; D005978:Glutathione / Q000378:metabolism; D021621:Imaging, Three-Dimensional; D007668:Kidney / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D018625:Microscopy, Atomic Force; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D051336:Mitochondrial Membranes / Q000378:metabolism; D053769:Nanocapsules / Q000737:chemistry* / Q000648:ultrastructure; D053758:Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D018384:Oxidative Stress / Q000187:drug effects*; D010316:Particle Size; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism; D012969:Sodium Fluoride / Q000633:toxicity*; D014783:Viscosity
chemical_list: D000975:Antioxidants; D001881:Borates; D002614:Chelating Agents; D053769:Nanocapsules; D017382:Reactive Oxygen Species; D002392:Catechin; D012969:Sodium Fluoride; D003094:Collagen; C010634:sodium borate; D005978:Glutathione
keywords: Catechin hydrate; Fluoride; Mitochondrial impairment; Nanocapsule; Oxidative stress; Sodium meta borate