title: Tumor necrosis factor-α and interleukin-6 gene polymorphism association with susceptibility to celiac disease in Italian patients.
abstract: The aim of this research was to study polymorphisms in the genes encoding cytokines interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) in patients with celiac disease (CD) antigens DQ2 (DQ2-positive) or DQ8 (DQ8-positive). We compared the results with healthy controls to determine whether any of the polymorphisms have a role in susceptibility to CD. A case-control of 192 patients with CD (96 DQ2-positive and 96 DQ8-positive) and 96 healthy controls from northeast Italy were included in the study. Analysis of single nucleotide polymorphisms (SNPs) was carried out using the polymerase chain reaction-restriction fragment length polymorphism method. Significant differences for the TNF-α(-308 G>A) polymorphism were observed when we compared the flowing groups: DQ2-positive with controls [odds ratio (OR) = 0.45, P = 0.0002]; DQ8-positive with controls (OR = 3.55, P < 0.0001); and DQ2-positive with DQ8-positive (OR = 0.12, P < 0.0001). We did not observe a statistically significant association between IL-6 (-174 G>C) polymorphism and CD (P > 0.05). Our results suggest that TNF-α(-308 G>A) polymorphism may play a role in susceptibility to CD in Italian patients.
authors.affiliation: Instituto de Ciências Biológicas, Universidade de Pernambuco, Recife, PE, Brasil.
authors.forename: R M
authors.initials: RM
authors.lastname: de Albuquerque Maranhão
authors.affiliation: Departamento de Genética, Universidade Federal de Pernambuco, Recife, PE, Brasil.
authors.forename: F A
authors.initials: FA
authors.lastname: Martins Esteves
authors.affiliation: Departamento de Genética, Universidade Federal de Pernambuco, Recife, PE, Brasil.
authors.forename: S
authors.initials: S
authors.lastname: Crovella
authors.affiliation: Istituto di Ricovero e Cura a Carattere Scientifico Materno Infantile Burlo Garofolo, Trieste, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Segat
authors.affiliation: Departamento de Biologia, Universidade Federal Rural de Pernambuco, Recife, PE, Brasil.
authors.forename: P R
authors.initials: PR
authors.lastname: Eleutério Souza
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000483:Alleles; D016022:Case-Control Studies; D002446:Celiac Disease / Q000453:epidemiology* / Q000235:genetics*; D002648:Child; D002675:Child, Preschool; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006683:HLA-DQ Antigens / Q000235:genetics; D006801:Humans; D015850:Interleukin-6 / Q000235:genetics*; D007558:Italy / Q000453:epidemiology; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*; D055815:Young Adult
chemical_list: D006683:HLA-DQ Antigens; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha