title: IL-1α-induced microvascular endothelial cells promote neutrophil killing by increasing MMP-9 concentration and lysozyme activity.
abstract: The recruitment of neutrophils by endothelial cells during infection has been extensively studied, but little is known about the regulation of neutrophils activity by endothelial cells. To examine the role of microvascular endothelial cells in neutrophil killing, we established a transmigration model using rat intestinal microvascular endothelial cells (RIMVECs) and measured the extracellular and intracellular killing of Escherichia coli, Lactobacillus acidophilus, and Staphylococcus aureus by transendothelial neutrophils. We observed that blood neutrophils engulfed bacteria but did not kill them, and lipopolysaccharide- or hemolysin-injured RIMVECs inhibited the extracellular and intracellular bactericidal activity of transendothelial neutrophils. In comparison, interleukin-1α-induced RIMVECs promoted the extracellular and intracellular killing activity of transendothelial neutrophils and significantly increased MMP-9 concentration and lysozyme activity in transendothelial neutrophils (p < 0.01 and p < 0.001, respectively). Our results demonstrated that activation of endothelial cells enhanced bactericidal activity of transendothelial neutrophils and bacterial toxin damage of endothelial cells led to reduction in bactericidal activity of transendothelial neutrophils. These findings offered new insight into the role of endothelial cells in the bactericidal activity of neutrophils.
authors.affiliation: Beijing Traditional Chinese Veterinary Engineering Center and Beijing Key Laboratory of Traditional Chinese Veterinary Medicine, Beijing University of Agriculture, No.7 BeiNong Road, Changping, Beijing, 102206, China.|National Center for Veterinary Drug Safety Evaluation, China Agricultural University, Beijing, 100193, China.
authors.forename: Xiaoye
authors.initials: X
authors.lastname: Liu
authors.affiliation: Beijing Traditional Chinese Veterinary Engineering Center and Beijing Key Laboratory of Traditional Chinese Veterinary Medicine, Beijing University of Agriculture, No.7 BeiNong Road, Changping, Beijing, 102206, China. donghong523@163.com.
authors.forename: Hong
authors.initials: H
authors.lastname: Dong
authors.affiliation: Beijing Traditional Chinese Veterinary Engineering Center and Beijing Key Laboratory of Traditional Chinese Veterinary Medicine, Beijing University of Agriculture, No.7 BeiNong Road, Changping, Beijing, 102206, China.
authors.forename: Mingming
authors.initials: M
authors.lastname: Wang
authors.affiliation: Department of Biology, Tennessee Center for Botanical Medicine Research, Middle Tennessee State University, Murfreesboro, TN, USA.
authors.forename: Ying
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Beijing Traditional Chinese Veterinary Engineering Center and Beijing Key Laboratory of Traditional Chinese Veterinary Medicine, Beijing University of Agriculture, No.7 BeiNong Road, Changping, Beijing, 102206, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Beijing Traditional Chinese Veterinary Engineering Center and Beijing Key Laboratory of Traditional Chinese Veterinary Medicine, Beijing University of Agriculture, No.7 BeiNong Road, Changping, Beijing, 102206, China.
authors.forename: Ge
authors.initials: G
authors.lastname: Hu
authors.affiliation: Beijing Traditional Chinese Veterinary Engineering Center and Beijing Key Laboratory of Traditional Chinese Veterinary Medicine, Beijing University of Agriculture, No.7 BeiNong Road, Changping, Beijing, 102206, China.
authors.forename: Huiqing
authors.initials: H
authors.lastname: Duan
authors.affiliation: Beijing Traditional Chinese Veterinary Engineering Center and Beijing Key Laboratory of Traditional Chinese Veterinary Medicine, Beijing University of Agriculture, No.7 BeiNong Road, Changping, Beijing, 102206, China. muxiang1109@sina.com.
authors.forename: Xiang
authors.initials: X
authors.lastname: Mu
mesh_terms: D000818:Animals; D001424:Bacterial Infections / Q000276:immunology* / Q000382:microbiology; D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic; D042783:Endothelial Cells / Q000276:immunology* / Q000382:microbiology; D005786:Gene Expression Regulation; D054884:Host-Pathogen Interactions; D053582:Interleukin-1alpha / Q000378:metabolism; D008070:Lipopolysaccharides / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism*; D009113:Muramidase / Q000378:metabolism; D009504:Neutrophils / Q000276:immunology*; D051381:Rats; D011919:Rats, Inbred Strains; D057705:Transendothelial and Transepithelial Migration / Q000276:immunology*
chemical_list: D053582:Interleukin-1alpha; D008070:Lipopolysaccharides; D009113:Muramidase; D020780:Matrix Metalloproteinase 9
keywords: Interleukin-1 alpha; Lysozyme; Matrix metalloprotein-9; Microvascular endothelial cells; Neutrophils