title: Using resonance light scattering and UV/vis absorption spectroscopy to study the interaction between gliclazide and bovine serum albumin.
abstract: At different temperatures (298, 310 and 318 K), the interaction between gliclazide and bovine serum albumin (BSA) was investigated using fluorescence quenching spectroscopy, resonance light scattering spectroscopy and UV/vis absorption spectroscopy. The first method studied changes in the fluorescence of BSA on addition of gliclazide, and the latter two methods studied the spectral change in gliclazide while BSA was being added. The results indicated that the quenching mechanism between BSA and gliclazide was static. The binding constant (Ka ), number of binding sites (n), thermodynamic parameters, binding forces and Hill's coefficient were calculated at three temperatures. Values for the binding constant obtained using resonance light scattering and UV/vis absorption spectroscopy were much greater than those obtained from fluorescence quenching spectroscopy, indicating that methods monitoring gliclazide were more accurate and reasonable. In addition, the results suggest that other residues are involved in the reaction and the mode 'point to surface' existed in the interaction between BSA and gliclazide. Copyright © 2015 John Wiley & Sons, Ltd.
authors.affiliation: Key Laboratory of Analytical Science and Technology of Hebei Province, College of Chemistry & Environmental Science, Hebei University, Baoding, People's Republic of China.
authors.forename: Qiu-Ju
authors.initials: QJ
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Analytical Science and Technology of Hebei Province, College of Chemistry & Environmental Science, Hebei University, Baoding, People's Republic of China.
authors.forename: Bao-Sheng
authors.initials: BS
authors.lastname: Liu
authors.affiliation: Key Laboratory of Analytical Science and Technology of Hebei Province, College of Chemistry & Environmental Science, Hebei University, Baoding, People's Republic of China.
authors.forename: Gai-Xia
authors.initials: GX
authors.lastname: Li
authors.affiliation: Key Laboratory of Analytical Science and Technology of Hebei Province, College of Chemistry & Environmental Science, Hebei University, Baoding, People's Republic of China.
authors.forename: Rong
authors.initials: R
authors.lastname: Han
mesh_terms: D000818:Animals; D002417:Cattle; D005907:Gliclazide / Q000737:chemistry*; D012542:Scattering, Radiation; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
chemical_list: D012710:Serum Albumin, Bovine; D005907:Gliclazide
keywords: UV/vis absorption spectroscopy; bovine serum albumin; fluorescence quenching spectroscopy; gliclazide; resonance light scattering spectroscopy