title: Kocuria arsenatissp. nov., an arsenic-resistant endophytic actinobacterium associated withProsopis laegivatagrown on high-arsenic-polluted mine tailing.
abstract: A Gram-stain-positive, aerobic, non-motile, coccoid, arsenic-resistant actinobacterial strain, designated CM1E1T, was isolated from the lateral root tissue ofProsopis laegivatagrown on a mine tailing in San Luis Potosi, Mexico. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CM1E1Twas clustered closely with species of the genusKocuria,and showed the highest sequence similarity of 98.7 % toKocuria rhizophilaTA68T. The DNA G+C content of strain CM1E1Twas 74.5 mol% (Tm). The major fatty acids were anteiso-C15 : 0, anteiso-C17 : 0and iso-C15 : 0. The peptidoglycan of the cell wall contained lysine and alanine. The major respiratory quinones were MK-7(H2) and MK-8(H2). On the basis of the phenotypic characterization, phylogenetic relationships and chemotaxonomic analyses, strain CM1E1Trepresents a novel species of the genusKocuria, for which the nameKocuria arsenatissp. nov. is proposed. The type strain is CM1E1T( = CCBAU 101092T= HAMBI 3625T= LMG 28671T).
authors.affiliation: Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, México D. F. 11340, México.
authors.forename: Brenda
authors.initials: B
authors.lastname: Román-Ponce
authors.affiliation: State Key Laboratory of Agrobiotechnology and College of Biological Sciences, China Agricultural University, Beijing 100193, PR China.
authors.forename: Dan
authors.initials: D
authors.lastname: Wang
authors.affiliation: Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, México D. F. 11340, México.
authors.forename: María
authors.initials: M
authors.lastname: Soledad Vásquez-Murrieta
authors.affiliation: State Key Laboratory of Agrobiotechnology and College of Biological Sciences, China Agricultural University, Beijing 100193, PR China.
authors.forename: Wen
authors.initials: W
authors.lastname: Feng Chen
authors.affiliation: Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, México D. F. 11340, México.
authors.forename: Paulina
authors.initials: P
authors.lastname: Estrada-de Los Santos
authors.affiliation: State Key Laboratory of Agrobiotechnology and College of Biological Sciences, China Agricultural University, Beijing 100193, PR China.
authors.forename: Xin
authors.initials: X
authors.lastname: Hua Sui
authors.affiliation: State Key Laboratory of Agrobiotechnology and College of Biological Sciences, China Agricultural University, Beijing 100193, PR China.|Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, México D. F. 11340, México.
authors.forename: En
authors.initials: E
authors.lastname: Tao Wang