title: Deinococcus actinosclerussp. nov., a novel bacterium isolated from soil of a rocky hillside.
abstract: Three Gram-stain-positive, catalase- and oxidase-positive coccus- or rod-shaped bacterial strains, designated BM2T, BM4 and BM5, were isolated from soil in South Korea. They showed strong resistance to gamma radiation with aD10value of 9 kGy but weak UVC resistance. The 16S rRNA sequences of strains BM2T, BM4 and BM5 represent a novel subline within the genusDeinococcusin the familyDeinococcaceae. The 16S rRNA gene sequences of strains BM2T, BM4 and BM5 were indistinguishable and showed 98.1-87.3 % similarity with other species of the genusDeinococcus. Strain BM2Texhibited relatively high levels of DNA-DNA hybridization with BM4 (87 ± 0.8 %) and BM5 (92 ± 1.2 %). Meanwhile, it showed a low level of DNA-DNA hybridization ( < 30 %) with other closely related species of the genusDeinococcus. The strains showed the typical chemotaxonomic characteristics of the genusDeinococcus, with the presence of menaquinone 8 as the respiratory quinone; the major fatty acids were summed feature 3 (composed of C16 : 1ω7c/C16 : 1ω6c), C15 : 1ω6cand C16 : 0. The DNA G+C content of strain BM2Twas 69.7 mol%. The polar lipid profile included major amounts of phosphatidylglycerol, phosphatidylcholine and an unknown aminolipid. On the basis of phenotypic and genotypic properties, and phylogenetic distinctiveness, strains BM2T, BM4 and BM5 should be classified in a novel species in the genusDeinococcus, for which the nameDeinococcus actinosclerussp. nov. is proposed. The type strain is BM2T( = KEMB 5401-184T= JCM 30700T); reference strains are BM4 ( = JCM 30701) and BM5 ( = JCM 30702).
authors.affiliation: Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University, Seoul 139-774, Korea.
authors.forename: Eun
authors.initials: E
authors.lastname: Sun Joo
authors.affiliation: Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University, Seoul 139-774, Korea.
authors.forename: Jae
authors.initials: J
authors.lastname: Jin Lee
authors.affiliation: Microorganism Resources Division, National Institute of Biological Resources, Incheon 404-107, Republic of Korea.
authors.forename: Myung-Suk
authors.initials: MS
authors.lastname: Kang
authors.affiliation: Radiation Research Division for Biotechnology, Korea Atomic Energy Research Institute, Jeongeup 580-185, Republic of Korea.
authors.forename: Sangyong
authors.initials: S
authors.lastname: Lim
authors.affiliation: Radiation Research Division for Biotechnology, Korea Atomic Energy Research Institute, Jeongeup 580-185, Republic of Korea.
authors.forename: Sun-Wook
authors.initials: SW
authors.lastname: Jeong
authors.affiliation: Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University, Seoul 139-774, Korea.
authors.forename: Eun
authors.initials: E
authors.lastname: Bit Kim
authors.affiliation: Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University, Seoul 139-774, Korea.
authors.forename: Seon
authors.initials: S
authors.lastname: Hwa Jeon
authors.affiliation: Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University, Seoul 139-774, Korea.
authors.forename: Sathiyaraj
authors.initials: S
authors.lastname: Srinivasan
authors.affiliation: Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University, Seoul 139-774, Korea.
authors.forename: Myung
authors.initials: M
authors.lastname: Kyum Kim