title: Optimization of magnetosome production by Acidithiobacillus ferrooxidans using desirability function approach.
abstract: Present study aimed to resolve the conflict between cell growth and magnetosome formation of Acidithiobacillus ferrooxidans (A. ferrooxidans) in batch experiments by applying response surface methodology (RSM) integrated a desirability function approach. The effects of several operating parameters on cell growth (OD600) and magnetosome production (Cmag) were evaluated. The maximum overall desirability (D) of 0.923 was achieved at iron concentration of 125.07mM, shake speed of 122.37rpm and nitrogen concentration of 2.40g/L. Correspondingly, the OD600 and Cmag were 0.522 and 1.196, respectively. The confirmation experiment confirmed that the optimum OD600 and Cmag obtained were in good agreement with the predicted values. The inductively coupled plasma atomic emission spectrometer (ICP-AES) and transmission electron microscopy (TEM) analyses revealed that the production of magnetosomes could be improved via optimization. X-ray diffraction (XRD) showed the magnetosomes are magnetite. Results indicated that RSM with a desirability function was a useful technique to get the maximum OD600 and Cmag simultaneously.
authors.affiliation: College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163319, PR China. Electronic address: hekouyanlei@gmail.com.
authors.forename: Lei
authors.initials: L
authors.lastname: Yan
authors.affiliation: College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163319, PR China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Zhang
authors.affiliation: School of Basic Medical Sciences, Ningxia Medical University, Yinchuan 750004, PR China.
authors.forename: Hetao
authors.initials: H
authors.lastname: Liu
authors.affiliation: College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163319, PR China.
authors.forename: Weidong
authors.initials: W
authors.lastname: Wang
authors.affiliation: School of Pharmacy, Lanzhou University, Lanzhou 730000, PR China.
authors.forename: Peng
authors.initials: P
authors.lastname: Chen
authors.affiliation: School of Pharmacy, Lanzhou University, Lanzhou 730000, PR China.
authors.forename: Hongyu
authors.initials: H
authors.lastname: Li
mesh_terms: D042763:Acidithiobacillus / Q000737:chemistry* / Q000378:metabolism*; D055944:Magnetosomes / Q000737:chemistry* / Q000378:metabolism*
keywords: Acidithiobacillus ferrooxidans; Desirability function; Magnetosome; Optimization; Response surface methodology