title: The anti-membranous glomerulonephritic activity of purified polysaccharides from Irpex lacteus Fr.
abstract: The present study aims to identify the major active component from mutant Irpex lacteus, which protects against cationic bovine serum albumin (C-BSA)-induced membranous glomerulonephropathy (MGN). The candidate component ILN3A (MW: 2264 kDa) was purified from mutant Irpex lacteus water extract. The backbone of ILN3A comprises (1→2) and (1→4) linkages, and (1)H NMR spectrum suggests the existence of α- and β-glycosidic anomeric carbon. In tissue culture study, ILN3A inhibits mesangial cell proliferation. In MGN rats, ILN3A reverses structural changes in kidney, suppresses abnormal high level of urine protein and restores concentration of serum albumin. ILN3A also reduces total cholesterol, triglycerides, and creatinine in serum, and 6-keto-PGF in kidney cortex. Further study shows ILN3A restores serum Interleukin 2, Interleukin 2 receptor, Interleukin 6, tumor necrosis factor α, and renal cortical nuclear factor kappa B. Our data shows ILN3A, the major active component of mutant Irpex lacteus, is a novel candidate anti-inflammatory medicine to treat MGN in clinics.
authors.affiliation: College of Life Science, Jilin University, Changchun 130012, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Wang
authors.affiliation: College of Life Science, Jilin University, Changchun 130012, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Song
authors.affiliation: College of Life Science, Jilin University, Changchun 130012, China.
authors.forename: Di
authors.initials: D
authors.lastname: Wang
authors.affiliation: College of Life Science, Jilin University, Changchun 130012, China.
authors.forename: Na
authors.initials: N
authors.lastname: Zhang
authors.affiliation: College of Life Science, Jilin University, Changchun 130012, China.
authors.forename: Jiahui
authors.initials: J
authors.lastname: Lu
authors.affiliation: College of Life Science, Jilin University, Changchun 130012, China.
authors.forename: Qingfan
authors.initials: Q
authors.lastname: Meng
authors.affiliation: College of Life Science, Jilin University, Changchun 130012, China.
authors.forename: Yulin
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: School of Chinese Medicine, The University of HongKong, 10 Sassoon Road, Pokfulam, HongKong, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Wang
authors.affiliation: Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin agricultural University, Changchun 130118, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: College of Life Science, Jilin University, Changchun 130012, China; The State Engineering Laboratory of AIDS Vaccine, Jilin University, Changchun 130012, China. Electronic address: jluwangdi@outlook.com.
authors.forename: Di
authors.initials: D
authors.lastname: Wang
authors.affiliation: College of Life Science, Jilin University, Changchun 130012, China; The State Engineering Laboratory of AIDS Vaccine, Jilin University, Changchun 130012, China. Electronic address: tenglesheng@jlu.edu.cn.
authors.forename: Lesheng
authors.initials: L
authors.lastname: Teng
mesh_terms: D000818:Animals; D001487:Basidiomycota / Q000737:chemistry*; D015415:Biomarkers / Q000097:blood; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D004195:Disease Models, Animal; D062610:Fungal Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D015433:Glomerulonephritis, Membranous / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology*; D007672:Kidney Cortex / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D050527:Mesangial Cells / Q000187:drug effects / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D011460:Prostaglandins F / Q000378:metabolism; D051381:Rats; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D015415:Biomarkers; D062610:Fungal Polysaccharides; D016328:NF-kappa B; D011460:Prostaglandins F
keywords: Glomerulonephritis; Nuclear factor kappa B; Polysaccharides of Irpex lacteus Fr.