title: Mass Spectrometric Quantification of Amphipathic, Polyphenolic Antioxidant of the Pacific Oyster (Crassostrea gigas).
abstract: A novel amphipathic phenolic compound, 3,5-dihydroxy-4-methoxybenzyl alcohol (DHMBA), that can be isolated from the Pacific oyster (Crassostrea gigas) has been found to protect human hepatocytes against oxidative stress. This study aims to establish a method for the measurement of DHMBA for industrial application. Liquid chromatography-tandem mass spectrometry using deuterated DHMBA as an internal standard and a polar end-capped ODS (Hypersil GOLD aQ) as the solid phase was validated. The limit of detection was 0.04 pmol (S/N = 5), and the limit of quantitation was 0.1 pmol (S/N = 10). The calibration curve was linear throughout the range of 0.1 - 16 pmol (r(2) = 0.9995). This method successfully quantified DHMBA in oysters from 11 sea areas in Japan. The results showed that the yield of DHMBA was variable from 9.8 to 58.8 μg g(-1) whole oyster meat wet weight but not affected by the seawater temperature. The proposed LC-MS/MS method is useful in quantitative studies for DHMBA and potentially for other amphipathic substances.
authors.affiliation: Faculty of Health Sciences, Hokkaido University.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Okabe
authors.forename: Shu-Ping
authors.initials: SP
authors.lastname: Hui
authors.forename: Hirotoshi
authors.initials: H
authors.lastname: Fuda
authors.forename: Takayuki
authors.initials: T
authors.lastname: Furukawa
authors.forename: Seiji
authors.initials: S
authors.lastname: Takeda
authors.forename: Rojeet
authors.initials: R
authors.lastname: Shrestha
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Miura
authors.forename: Mitsugu
authors.initials: M
authors.lastname: Watanabe
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Chiba
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000032:analysis*; D001592:Benzyl Alcohols / Q000032:analysis*; D002138:Calibration; D002853:Chromatography, Liquid / Q000379:methods*; D049896:Crassostrea / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D057230:Limit of Detection; D059808:Polyphenols / Q000032:analysis*; D012015:Reference Standards; D015203:Reproducibility of Results; D012623:Seawater / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013696:Temperature
chemical_list: C000611248:3,5-dihydroxy-4-methoxybenzyl alcohol; D000975:Antioxidants; D001592:Benzyl Alcohols; D059808:Polyphenols; C026679:octadecylsilica; D012822:Silicon Dioxide; D006108:Graphite