title: Efficiency of Base Excision Repair of Oxidative DNA Damage and Its Impact on the Risk of Colorectal Cancer in the Polish Population.
abstract: DNA oxidative lesions are widely considered as a potential risk factor for colorectal cancer development. The aim of this work was to determine the role of the efficiency of base excision repair, both in lymphocytes and in epithelial tissue, in patients with CRC and healthy subjects. SNPs were identified within genes responsible for steps following glycosylase action in BER, and patients and healthy subjects were genotyped. A radioisotopic BER assay was used for assessing repair efficiency and TaqMan for genotyping. Decreased BER activity was observed in lymphocyte extract from CRC patients and in cancer tissue extract, compared to healthy subjects. In addition, polymorphisms of EXO1, LIG3, and PolB may modulate the risk of colorectal cancer by decreasing (PolB) or increasing (LIG3 and EXO1) the chance of malignant transformation.
authors.affiliation: Department of Clinical Chemistry and Biochemistry, Medical University of Lodz, Plac Hallera 1, 90-647 Lodz, Poland.
authors.forename: J
authors.initials: J
authors.lastname: Kabzinski
authors.affiliation: Department of Clinical Chemistry and Biochemistry, Medical University of Lodz, Plac Hallera 1, 90-647 Lodz, Poland.
authors.forename: B
authors.initials: B
authors.lastname: Mucha
authors.affiliation: Department of Clinical Chemistry and Biochemistry, Medical University of Lodz, Plac Hallera 1, 90-647 Lodz, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Cuchra
authors.affiliation: Department of Clinical Chemistry and Biochemistry, Medical University of Lodz, Plac Hallera 1, 90-647 Lodz, Poland.
authors.forename: L
authors.initials: L
authors.lastname: Markiewicz
authors.affiliation: Department of Clinical Chemistry and Biochemistry, Medical University of Lodz, Plac Hallera 1, 90-647 Lodz, Poland.
authors.forename: K
authors.initials: K
authors.lastname: Przybylowska
authors.affiliation: Department of General and Colorectal Surgery, Medical University of Lodz, Plac Hallera 1, 90-647 Lodz, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Dziki
authors.affiliation: Department of General and Colorectal Surgery, Medical University of Lodz, Plac Hallera 1, 90-647 Lodz, Poland.
authors.forename: L
authors.initials: L
authors.lastname: Dziki
authors.affiliation: Department of Clinical Chemistry and Biochemistry, Medical University of Lodz, Plac Hallera 1, 90-647 Lodz, Poland.
authors.forename: I
authors.initials: I
authors.lastname: Majsterek
mesh_terms: D000368:Aged; D015179:Colorectal Neoplasms / Q000235:genetics*; D004249:DNA Damage; D004260:DNA Repair; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000235:genetics*; D011044:Poland; D020641:Polymorphism, Single Nucleotide
chemical_list: D009363:Neoplasm Proteins