title: Continuous cellularization of calcium phosphate hybrid scaffolds induced by plasma polymer activation.
abstract: The generation of hybrid materials based on β-tricalcium phosphate (TCP) and various biodegradable polymers like poly(l-lactide-co-d,l-lactide) (PLA) represents a common approach to overcoming the disadvantages of pure TCP devices. These disadvantages lie in TCP's mechanical properties, such as brittleness. The positive characteristic of PLA - improvement of compressive strength of calcium phosphate scaffolds - is diametrically opposed to its cell attractiveness. Therefore, the objective of this work was to optimize osteoblast migration and cellularization inside a three-dimensionally (3D) printed, PLA polymer stabilized TCP hybrid scaffold by a plasma polymer process depositing amino groups via allylamine. MG-63 osteoblastic cells inside the 10mm hybrid scaffold were dynamically cultivated for 14days in a 3D model system integrated in a perfusion reactor. The whole TCP/PLA hybrid scaffold was continuously colonized due to plasma polymerized allylamine activation inducing the migration potential of osteoblasts.
authors.affiliation: University Medical Center Rostock, Cell Biology, Schillingallee 69, D-18057 Rostock, Germany.
authors.forename: Claudia
authors.initials: C
authors.lastname: Bergemann
authors.affiliation: University of Rostock, Fluid Technology and Microfluidics, Justus-von-Liebig Weg 6, D-18059 Rostock, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Cornelsen
authors.affiliation: Leibniz-Institute for Plasma Science and Technology (INP), Felix-Hausdorff-Str. 2, D-17489 Greifswald, Germany.
authors.forename: Antje
authors.initials: A
authors.lastname: Quade
authors.affiliation: INNOVENT e.V., Biomaterials Department, Pruessingstrasse 27B, D-07745 Jena, Germany.
authors.forename: Thorsten
authors.initials: T
authors.lastname: Laube
authors.affiliation: INNOVENT e.V., Biomaterials Department, Pruessingstrasse 27B, D-07745 Jena, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Schnabelrauch
authors.affiliation: University Medical Center Rostock, Cell Biology, Schillingallee 69, D-18057 Rostock, Germany.
authors.forename: Henrike
authors.initials: H
authors.lastname: Rebl
authors.affiliation: Institute for Polymer Technologies (IPT) e.V., Alter Holzhafen 19, D-23966 Wismar, Germany.
authors.forename: Volker
authors.initials: V
authors.lastname: Weißmann
authors.affiliation: University of Rostock, Fluid Technology and Microfluidics, Justus-von-Liebig Weg 6, D-18059 Rostock, Germany.
authors.forename: Hermann
authors.initials: H
authors.lastname: Seitz
authors.affiliation: University Medical Center Rostock, Cell Biology, Schillingallee 69, D-18057 Rostock, Germany. Electronic address: barbara.nebe@med.uni-rostock.de.
authors.forename: Barbara
authors.initials: B
authors.lastname: Nebe
mesh_terms: D002130:Calcium Phosphates / Q000737:chemistry*; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D006801:Humans; D058626:Plasma Gases; D011091:Polyesters / Q000737:chemistry* / Q000494:pharmacology*; D066330:Printing, Three-Dimensional; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D002130:Calcium Phosphates; D058626:Plasma Gases; D011091:Polyesters; C033616:poly(lactide); C020243:calcium phosphate
keywords: 3D printing; Calcium phosphate; Cell migration; Human osteoblasts; Perfusion cell reactor; Plasma technology; Tissue engineering