title: Changes in global gene expression profiles induced by HPV 16 E6 oncoprotein variants in cervical carcinoma C33-A cells.
abstract: We analyzed the effects of the expression of HPV 16 E6 oncoprotein variants (AA-a, AA-c, E-A176/G350, E-C188/G350, E-G350), and the E-Prototype in global gene expression profiles in an in vitro model. E6 gene was cloned into an expression vector fused to GFP and was transfected in C33-A cells. Affymetrix GeneChip Human Transcriptome Array 2.0 platform was used to analyze the expression of over 245,000 coding transcripts. We found that HPV16 E6 variants altered the expression of 387 different genes in comparison with E-Prototype. The altered genes are involved in cellular processes related to the development of cervical carcinoma, such as adhesion, angiogenesis, apoptosis, differentiation, cell cycle, proliferation, transcription and protein translation. Our results show that polymorphic changes in HPV16 E6 natural variants are sufficient to alter the overall gene expression profile in C33-A cells, explaining in part the observed differences in oncogenic potential of HPV16 variants.
authors.affiliation: Laboratorio de Biomedicina Molecular, Unidad Académica de Ciencias Químico Biológicas, Universidad Autónoma de Guerrero, Chilpancingo, Gro., México. Electronic address: zak_ana@yahoo.com.mx.
authors.forename: Ana Elvira
authors.initials: AE
authors.lastname: Zacapala-Gómez
authors.affiliation: Laboratorio de Biomedicina Molecular, Unidad Académica de Ciencias Químico Biológicas, Universidad Autónoma de Guerrero, Chilpancingo, Gro., México. Electronic address: odelmoralh@gmail.com.
authors.forename: Oscar
authors.initials: O
authors.lastname: Del Moral-Hernández
authors.affiliation: Departamento de Biomedicina Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), México, D.F., México. Electronic address: nvillega@cinvestav.mx.
authors.forename: Nicolás
authors.initials: N
authors.lastname: Villegas-Sepúlveda
authors.affiliation: Laboratorio de Genómica del Cáncer, Instituto Nacional de Medicina Genómica (INMEGEN), México, D.F., México. Electronic address: ahidalgo@inmegen.gob.mx.
authors.forename: Alfredo
authors.initials: A
authors.lastname: Hidalgo-Miranda
authors.affiliation: Laboratorio de Genómica del Cáncer, Instituto Nacional de Medicina Genómica (INMEGEN), México, D.F., México. Electronic address: sromero_cordoba@hotmail.com.
authors.forename: Sandra Lorena
authors.initials: SL
authors.lastname: Romero-Córdoba
authors.affiliation: Laboratorio de Genómica del Cáncer, Instituto Nacional de Medicina Genómica (INMEGEN), México, D.F., México. Electronic address: frebeltran@hotmail.com.
authors.forename: Fredy Omar
authors.initials: FO
authors.lastname: Beltrán-Anaya
authors.affiliation: Laboratorio de Biomedicina Molecular, Unidad Académica de Ciencias Químico Biológicas, Universidad Autónoma de Guerrero, Chilpancingo, Gro., México. Electronic address: leyvamarco13@gmail.com.
authors.forename: Marco Antonio
authors.initials: MA
authors.lastname: Leyva-Vázquez
authors.affiliation: Laboratorio de Investigación en Citopatología e Histoquímica, Unidad Académica de Ciencias Químico Biológicas, Universidad Autónoma de Guerrero, Chilpancingo, Gro., México. Electronic address: uzdelcarmen14@gmail.com.
authors.forename: Luz Del Carmen
authors.initials: Ldel C
authors.lastname: Alarcón-Romero
authors.affiliation: Laboratorio de Biomedicina Molecular, Unidad Académica de Ciencias Químico Biológicas, Universidad Autónoma de Guerrero, Chilpancingo, Gro., México. Electronic address: b.illadesaguiar@gmail.com.
authors.forename: Berenice
authors.initials: B
authors.lastname: Illades-Aguiar
mesh_terms: D019677:Artificial Gene Fusion; D045744:Cell Line, Tumor; D004847:Epithelial Cells / Q000821:virology*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000235:genetics; D054884:Host-Pathogen Interactions; D052162:Human papillomavirus 16 / Q000235:genetics*; D006801:Humans; D046228:Microarray Analysis; D020411:Oligonucleotide Array Sequence Analysis; D009856:Oncogene Proteins, Viral / Q000096:biosynthesis / Q000235:genetics*; D011110:Polymorphism, Genetic; D012097:Repressor Proteins / Q000096:biosynthesis / Q000235:genetics*; D059467:Transcriptome
chemical_list: C068651:E6 protein, Human papillomavirus type 16; D009856:Oncogene Proteins, Viral; D012097:Repressor Proteins; D049452:Green Fluorescent Proteins
keywords: C33-A cells; Cervical carcinoma; Global gene expression; HPV 16 E6 oncoprotein variants; HPV 16 variants