title: Superoxide dismutase and taurine supplementation improves in vitro blastocyst yield from poor-quality feline oocytes.
abstract: Blastocyst production in vitro seems to be crucial part of assisted reproduction techniques in feline species. However, the results of cats' oocyte maturation and embryo development are still lower than those in other species. The aim of this study was to evaluate whether the supplementation with superoxide dismutase (SOD) and taurine during maturation or culture would improve the blastocyst yield obtained from lower grades of oocytes, that are usually discarded, as not suitable for further in vitro purposes. To investigate the effect of antioxidants' addition, the good- and poor-quality oocytes, were cultured with the addition of 10-mmol taurine and 600 UI/mL SOD. The nuclear maturity, embryo development, and blastocyst quality were subsequently assessed. In control group, without antioxidant supplementation, significantly less poor-quality oocytes matured (42% vs. 62%) and more degenerated (35% vs. 20%), comparing to the experimental group supplemented with SOD and taurine. The amount of obtained blastocyst was much higher, when poor quality oocytes were supplemented with SOD and taurine (supplementation to IVM-4%; supplementation to IVC-5.5%; supplementation to IVM and IVC-5.9% of blastocyst), comparing to not supplemented control group (1.3%). The best blastocysts were obtained when poor oocytes had antioxidants added only during embryo culture (185 ± 13.4 blastomeres vs. 100 ± 1.5 in control). In the present study, we reported that the lower grades of oocytes can better mature and form significantly more blastocysts with better quality, when cultured with addition of SOD and taurine.
authors.affiliation: Faculty of Veterinary Medicine, Department of Reproduction and Clinic of Farm Animals, Wroclaw University of Environmental and Life Sciences, Wrocław, Poland. Electronic address: malgorzata.ochota@up.wroc.pl.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Ochota
authors.affiliation: Faculty of Veterinary Medicine, Department of Reproduction and Clinic of Farm Animals, Wroclaw University of Environmental and Life Sciences, Wrocław, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Pasieka
authors.affiliation: Faculty of Veterinary Medicine, Department of Reproduction and Clinic of Farm Animals, Wroclaw University of Environmental and Life Sciences, Wrocław, Poland.
authors.forename: Wojciech
authors.initials: W
authors.lastname: Niżański
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001755:Blastocyst / Q000166:cytology / Q000187:drug effects*; D002415:Cats; D018929:Cell Culture Techniques / Q000379:methods / Q000662:veterinary; D002478:Cells, Cultured; D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology; D047108:Embryonic Development / Q000187:drug effects; D005260:Female; D005307:Fertilization in Vitro / Q000662:veterinary; D059471:In Vitro Oocyte Maturation Techniques / Q000379:methods* / Q000662:veterinary; D008297:Male; D009865:Oocytes / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D013482:Superoxide Dismutase / Q000494:pharmacology*; D013654:Taurine / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D003470:Culture Media; D013654:Taurine; D013482:Superoxide Dismutase
keywords: Blastocyst production; Cat; Oocyte maturation; Superoxide dismutase; Taurine