title: Long Term Influence of Carbon Nanoparticles on Health and Liver Status in Rats.
abstract: Due to their excellent biocompatibility, carbon nanoparticles have been widely investigated for prospective biomedical applications. However, their impact on an organism with prolonged exposure is still not well understood. Here, we performed an experiment investigating diamond, graphene oxide and graphite nanoparticles, which were repeatedly administrated intraperitoneally into Wistar rats for four weeks. Some of the animals was sacrificed after the last injection, whereas the rest were sacrificed twelve weeks after the last exposure. We evaluated blood morphology and biochemistry, as well as the redox and inflammatory state of the liver. The results show the retention of nanoparticles within the peritoneal cavity in the form of prominent aggregates in proximity to the injection site, as well as the presence of some nanoparticles in the mesentery. Small aggregates were also visible in the liver serosa, suggesting possible transportation to the liver. However, none of the tested nanoparticles affected the health of animals. This lack of toxic effect may suggest the potential applicability of nanoparticles as drug carriers for local therapies, ensuring accumulation and slow release of drugs into a targeted tissue without harmful systemic side effects.
authors.affiliation: Division of Nanobiotechnology, Warsaw University of Life Sciences, Warsaw, Poland.
authors.forename: Barbara
authors.initials: B
authors.lastname: Strojny
authors.affiliation: Division of Nanobiotechnology, Warsaw University of Life Sciences, Warsaw, Poland.
authors.forename: Natalia
authors.initials: N
authors.lastname: Kurantowicz
authors.affiliation: Division of Nanobiotechnology, Warsaw University of Life Sciences, Warsaw, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Sawosz
authors.affiliation: Division of Nanobiotechnology, Warsaw University of Life Sciences, Warsaw, Poland.
authors.forename: Marta
authors.initials: M
authors.lastname: Grodzik
authors.affiliation: Division of Nanobiotechnology, Warsaw University of Life Sciences, Warsaw, Poland.
authors.forename: Sławomir
authors.initials: S
authors.lastname: Jaworski
authors.affiliation: Division of Nanobiotechnology, Warsaw University of Life Sciences, Warsaw, Poland.
authors.forename: Marta
authors.initials: M
authors.lastname: Kutwin
authors.affiliation: Division of Nanobiotechnology, Warsaw University of Life Sciences, Warsaw, Poland.
authors.forename: Mateusz
authors.initials: M
authors.lastname: Wierzbicki
authors.affiliation: Division of Nanobiotechnology, Warsaw University of Life Sciences, Warsaw, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Hotowy
authors.affiliation: Department of Chemical Technologies, Institute of Electronic Materials Technology, Warsaw, Poland.
authors.forename: Ludwika
authors.initials: L
authors.lastname: Lipińska
authors.affiliation: Department of Veterinary Clinical and Animal Sciences, University of Copenhagen, Copenhagen, Denmark.
authors.forename: André
authors.initials: A
authors.lastname: Chwalibog
mesh_terms: D000818:Animals; D002244:Carbon / Q000008:administration & dosage* / Q000493:pharmacokinetics; D018130:Diamond / Q000008:administration & dosage / Q000493:pharmacokinetics; D004337:Drug Carriers / Q000008:administration & dosage* / Q000493:pharmacokinetics; D006108:Graphite / Q000008:administration & dosage / Q000493:pharmacokinetics; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D053758:Nanoparticles / Q000008:administration & dosage* / Q000009:adverse effects / Q000737:chemistry; D010529:Peritoneal Cavity; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
chemical_list: D004337:Drug Carriers; D002244:Carbon; D018130:Diamond; D006108:Graphite