title: Antimicrobial activity and cytocompatibility of silver nanoparticles coated catheters via a biomimetic surface functionalization strategy.
abstract: Catheter-related bloodstream infections are a significant problem in the clinic and may result in a serious infection. Here, we developed a facile and green procedure for buildup of silver nanoparticles (AgNPs) on the central venous catheters (CVCs) surface. Inspired by mussel adhesive proteins, dopamine was used to form a thin polydopamine layer and induce AgNPs formation without additional reductants or stabilizers. The chemical and physicochemical properties of AgNPs coated CVCs were characterized by scanning electron microscopy, X-ray photoelectron spectroscopy, Raman spectroscopy, and water contact angle. The Staphylococcus aureus culture experiment was used to study the antibacterial properties. The cytocompatibility was assessed by water soluble tetrazolium salts (WST-1) assay, fluorescence staining, and scanning electron microscopy analysis. The results indicated that the CVCs surface was successfully coated with compact AgNPs. AgNPs were significantly well separated and spherical with a size of 30-50 nm. The density of AgNPs could be modulated by the concentration of silver nitrate solution. The antibacterial activity was dependent on the AgNPs dose. The high dose of AgNPs showed excellent antibacterial activity while associated with increased cytotoxicity. The appropriate density of AgNPs coated CVCs could exhibit improved biocompatibility and maintained evident sterilization effect. It is promising to design mussel-inspired silver releasing CVCs with both significant antimicrobial efficacy and appropriate biological safety.
authors.affiliation: Department of Cardiology, The Affiliated Dongnan Hospital of Xiamen University, Zhangzhou, People's Republic of China.
authors.forename: Ke
authors.initials: K
authors.lastname: Wu
authors.affiliation: Department of Medical Materials, Beijing Medical Implant Engineering Research Center, Beijing Naton Technology Group, Beijing, People's Republic of China ; State Key Laboratory of Physical Chemistry of Solid Surfaces, Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, People's Republic of China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Medical Materials, Beijing Medical Implant Engineering Research Center, Beijing Naton Technology Group, Beijing, People's Republic of China ; State Key Laboratory of Physical Chemistry of Solid Surfaces, Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, People's Republic of China.
authors.forename: Yanmei
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Cardiology, The Affiliated Dongnan Hospital of Xiamen University, Zhangzhou, People's Republic of China.
authors.forename: Jiexi
authors.initials: J
authors.lastname: Deng
authors.affiliation: Department of Medical Materials, Beijing Medical Implant Engineering Research Center, Beijing Naton Technology Group, Beijing, People's Republic of China ; State Key Laboratory of Physical Chemistry of Solid Surfaces, Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, People's Republic of China.
authors.forename: Changjian
authors.initials: C
authors.lastname: Lin
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D040761:Biomimetic Materials / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D062905:Central Venous Catheters; D020099:Coated Materials, Biocompatible / Q000494:pharmacology*; D053768:Metal Nanoparticles / Q000737:chemistry*; D051379:Mice; D008826:Microbial Sensitivity Tests; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D056951:Photoelectron Spectroscopy; D012834:Silver / Q000494:pharmacology*; D013059:Spectrum Analysis, Raman; D013211:Staphylococcus aureus / Q000187:drug effects; D014867:Water
chemical_list: D000890:Anti-Infective Agents; D020099:Coated Materials, Biocompatible; D014867:Water; D012834:Silver
keywords: antibacterial activity; bio-compatibility; central venous catheter; surface modification