title: A 3-day EGCG-supplementation reduces interstitial lactate concentration in skeletal muscle of overweight subjects.
abstract: Green tea, particularly epigallocatechin-3-gallate (EGCG), may affect body weight and composition, possibly by enhancing fat oxidation. The aim of this double-blind, randomized placebo-controlled cross-over study was to investigate whether 3-day supplementation with EGCG (282 mg/day) stimulates fat oxidation and lipolysis in 24 overweight subjects (age = 30 ± 2 yrs, BMI = 27.7 ± 0.3 kg/m(2)). Energy expenditure, substrate metabolism and circulating metabolites were determined during fasting and postprandial conditions. After 6 h, a fat biopsy was collected to examine gene expression. In 12 subjects, skeletal muscle glycerol, glucose and lactate concentrations were determined using microdialysis. EGCG-supplementation did not alter energy expenditure and substrate oxidation compared to placebo. Although EGCG reduced postprandial circulating glycerol concentrations (P = 0.015), no difference in skeletal muscle lipolysis was observed. Fasting (P = 0.001) and postprandial (P = 0.003) skeletal muscle lactate concentrations were reduced after EGCG-supplementation compared to placebo, despite similar tissue blood flow. Adipose tissue leptin (P = 0.05) and FAT/CD36 expression (P = 0.08) were increased after EGCG compared to placebo. In conclusion, 3-day EGCG-supplementation decreased postprandial plasma glycerol concentrations, but had no significant effects on skeletal muscle lipolysis and whole-body fat oxidation in overweight individuals. Furthermore, EGCG decreased skeletal muscle lactate concentrations, which suggest a shift towards a more oxidative muscle phenotype.
authors.affiliation: Department of Human Biology, NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University Medical Centre+, Maastricht, The Netherlands.
authors.forename: Jasper
authors.initials: J
authors.lastname: Most
authors.affiliation: Department of Human Biology, NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University Medical Centre+, Maastricht, The Netherlands.
authors.forename: Judith G P
authors.initials: JG
authors.lastname: van Can
authors.affiliation: Department of Human Movement Sciences, NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University Medical Centre, Maastricht, The Netherlands.
authors.forename: Jan-Willem
authors.initials: JW
authors.lastname: van Dijk
authors.affiliation: Department of Human Biology, NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University Medical Centre+, Maastricht, The Netherlands.
authors.forename: Gijs H
authors.initials: GH
authors.lastname: Goossens
authors.affiliation: Department of Human Biology, NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University Medical Centre+, Maastricht, The Netherlands.
authors.forename: Johan
authors.initials: J
authors.lastname: Jocken
authors.affiliation: DSM Nutritional Products, Basel, Switzerland.
authors.forename: Jeannette J
authors.initials: JJ
authors.lastname: Hospers
authors.affiliation: DSM Nutritional Products, Basel, Switzerland.
authors.forename: Igor
authors.initials: I
authors.lastname: Bendik
authors.affiliation: Department of Human Biology, NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University Medical Centre+, Maastricht, The Netherlands.
authors.forename: Ellen E
authors.initials: EE
authors.lastname: Blaak
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism; D002392:Catechin / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000378:metabolism; D019587:Dietary Supplements; D004734:Energy Metabolism; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D019344:Lactic Acid / Q000378:metabolism*; D008066:Lipolysis; D008297:Male; D055442:Metabolome; D055432:Metabolomics / Q000379:methods; D018482:Muscle, Skeletal / Q000378:metabolism*; D050177:Overweight / Q000235:genetics / Q000378:metabolism*; D010084:Oxidation-Reduction; D013997:Time Factors
chemical_list: D019344:Lactic Acid; D002392:Catechin; C045651:epigallocatechin gallate