title: Powdered coconut water as a storage medium to preserve the viability of periodontal ligament cells: a laboratory study.
abstract: OBJECTIVE
  To investigate the ability of newly developed powdered coconut water formulas (ACP) with different osmolarities to maintain the viability of periodontal ligament (PDL) cells over time compared with other solutions.
  
  
  METHODS
  Dogs teeth were extracted and stored for two periods, 3 h or 24 h, in the following media: long-shelf life CW (CW), pH-adjusted long-shelf life CW (pH-CW) and powdered CW that was pH and osmolality adjusted (ACP-404-I, 250 mOsm kg-1H2O; pH 7.0; ACP-404-II, 372 mOsm kg-1H2O; pH 7.0; ACP-404-III, 300 mOsm kg-1H2O; pH 7.4). The positive control group (Pc) corresponded to immediate measurement after tooth extraction, and two negative controls (Nc) corresponded to 3 h and 24 h of dry time. PDL cells were extracted, and cell viability analysed by Trypan blue exclusion. Data were analysed statistically using two-way anova followed by the Tukey test and one-way anova followed by the Dunnett test (P < 0.05).
  
  
  RESULTS
  At 3 h and 24 h, ACP-404-I had a performance similar to those of ACP-404-II and pH-CW, with significantly higher (P = 0.004) percentages of viable cells than ACP-404-III and CW. The positive control group had a significantly higher (P = 0.002) percentage of viable cells than the negative control groups, CW and ACP-404-III, irrespective of the period evaluated.
  
  
  CONCLUSIONS
  Powdered coconut water formulas, ACP-404-I and ACP-404-II, preserved viability for up to 24 h.
authors.affiliation: Department of Endodontics, Federal University of Uberlândia, Uberlândia, Brazil.
authors.forename: C C G
authors.initials: CC
authors.lastname: Moura
authors.affiliation: Department of Oral and Maxillofacial Surgery and Implantology, Federal University of Uberlândia, Uberlândia, Brazil.
authors.forename: P B F
authors.initials: PB
authors.lastname: Soares
authors.affiliation: Department of Operative Dentistry and Dental Materials, Federal University of Uberlândia, Uberlândia, Brazil.
authors.forename: M V P
authors.initials: MV
authors.lastname: Reis
authors.affiliation: Department of Morphology, Biomedical Sciences Institute, Federal University of Uberlândia, Uberlândia, Brazil.
authors.forename: P
authors.initials: P
authors.lastname: Dechichi
authors.affiliation: Veterinary Sciences Institute, Ceará State University, Fortaleza, Brazil.
authors.forename: C C M
authors.initials: CC
authors.lastname: Salgueiro
authors.affiliation: Veterinary Sciences Institute, Ceará State University, Fortaleza, Brazil.
authors.forename: M H N R
authors.initials: MH
authors.lastname: Sobral
authors.affiliation: Department of Oral and Maxillofacial Surgery and Implantology, Federal University of Uberlândia, Uberlândia, Brazil.
authors.forename: D
authors.initials: D
authors.lastname: Zanetta Barbosa
authors.affiliation: Department of Operative Dentistry and Dental Materials, Federal University of Uberlândia, Uberlândia, Brazil.
authors.forename: C J
authors.initials: CJ
authors.lastname: Soares
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects*; D003059:Cocos; D004285:Dogs; D019188:Organ Preservation Solutions / Q000494:pharmacology*; D009994:Osmolar Concentration; D010513:Periodontal Ligament / Q000166:cytology*; D011208:Powders
chemical_list: D019188:Organ Preservation Solutions; D011208:Powders
keywords: cell viability; coconut water; storage media; tooth avulsion