title: Artificial polyepitope HIV-1 immunogen containing mimotope of 2F5 epitope.
abstract: Constructing a vaccine against HIV-1, able to induce production of broadly neutralizing antibodies, is crucial. We report here the selection and characterization of RDWSFDRWSLSEFWL peptide mimotope that binds specifically to bNAbs 2F5. The peptide mimotope was selected from 15-mer phage-displayed peptide library by using Mab 2F5 as the selecting agent. The most abundant RDWSFDRWSLSEFWL peptide was inserted into a carrier, an artificial polyepitope immunogen - TBI (T- and B-cell immunogen). TBI-2F5 polyepitope immunogen that includes the mimotope of 2F5 epitope was constructed. It was shown that sera of mice immunized with TBI-2F5 protein recognized TBI protein as well as RDWSFDRWSLSEFWL peptide. The capacity of sera of immunized mice to neutralize HIV-1 was demonstrated using subtype B env-pseudoviruses of HIV-1 QH0692.42 and PVO.4. Based on these results, we conclude that peptide mimotope of 2F5 epitope RDWSFDRWSLSEFWL can be an essential component for a successful HIV-vaccine.
authors.affiliation: SRC VB Vector, Koltsovo, the Novosibirsk region, Russia. shherbakova_ns@vector.nsc.ru.
authors.forename: Nadezhda S
authors.initials: NS
authors.lastname: Shcherbakova
authors.forename: Dmitry N
authors.initials: DN
authors.lastname: Shcherbakov
authors.forename: Anastasiya Yu
authors.initials: AY
authors.lastname: Bakulina
authors.forename: Larisa I
authors.initials: LI
authors.lastname: Karpenko
authors.forename: Alexander B
authors.initials: AB
authors.lastname: Ryzhikov
authors.forename: Alexander A
authors.initials: AA
authors.lastname: Ilyichev
mesh_terms: D016915:AIDS Vaccines / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology; D057134:Antibodies, Neutralizing / Q000276:immunology; D000080908:Broadly Neutralizing Antibodies; D000939:Epitopes / Q000737:chemistry / Q000276:immunology*; D015483:HIV Antibodies; D015497:HIV-1 / Q000276:immunology* / Q000472:pathogenicity; D006801:Humans; D051379:Mice; D014614:Vaccines, Synthetic / Q000737:chemistry / Q000276:immunology*
chemical_list: C558560:2F5 monoclonal antibody; D016915:AIDS Vaccines; D000911:Antibodies, Monoclonal; D057134:Antibodies, Neutralizing; D000080908:Broadly Neutralizing Antibodies; D000939:Epitopes; D015483:HIV Antibodies; D014614:Vaccines, Synthetic