title: Impaired antiviral response of adenovirus-transformed cell lines supports virus replication.
abstract: Activation of the innate immune response represents one of the most important cellular mechanisms to limit virus replication and spread in cell culture. Here, we examined the effect of adenoviral gene expression on the antiviral response in adenovirus-transformed cell lines; HEK293, HEK293SF and AGE1.HN. We demonstrate that the expression of the early region protein 1A in these cell lines impairs their ability to activate antiviral genes by the IFN pathway. This property may help in the isolation of newly emerging viruses and the propagation of interferon-sensitive virus strains.
authors.affiliation: Max Planck Institute for Dynamics of Complex Technical Systems, Sandtorstrasse 1, 39106 Magdeburg, Germany.
authors.forename: Mandy
authors.initials: M
authors.lastname: Bachmann
authors.affiliation: Max Planck Institute for Dynamics of Complex Technical Systems, Sandtorstrasse 1, 39106 Magdeburg, Germany.
authors.forename: Theresa
authors.initials: T
authors.lastname: Breitwieser
authors.affiliation: Helmholtz Center for Infection Research, Inhoffenstrasse 7, 38124 Braunschweig, Germany.
authors.forename: Christoph
authors.initials: C
authors.lastname: Lipps
authors.affiliation: Helmholtz Center for Infection Research, Inhoffenstrasse 7, 38124 Braunschweig, Germany.
authors.forename: Dagmar
authors.initials: D
authors.lastname: Wirth
authors.affiliation: ProBioGen AG, Goethestrasse 54, 13086 Berlin, Germany.
authors.forename: Ingo
authors.initials: I
authors.lastname: Jordan
authors.affiliation: Max Planck Institute for Dynamics of Complex Technical Systems, Sandtorstrasse 1, 39106 Magdeburg, Germany.|Otto von Guericke University Magdeburg, Universitaetsplatz 2, 39106 Magdeburg, Germany.
authors.forename: Udo
authors.initials: U
authors.lastname: Reichl
authors.affiliation: Otto von Guericke University Magdeburg, Universitaetsplatz 2, 39106 Magdeburg, Germany.|Max Planck Institute for Dynamics of Complex Technical Systems, Sandtorstrasse 1, 39106 Magdeburg, Germany.
authors.forename: Timo
authors.initials: T
authors.lastname: Frensing
mesh_terms: D000256:Adenoviridae / Q000276:immunology* / Q000502:physiology*; D002461:Cell Line, Transformed; D006801:Humans; D057131:Immune Evasion; D007113:Immunity, Innate; D014764:Viral Proteins / Q000096:biosynthesis / Q000276:immunology; D014779:Virus Replication
chemical_list: D014764:Viral Proteins