title: [Transfected miR-1908 inhibits renal fibrosis via targeting transforming growth factor beta 1].
abstract: OBJECTIVE
  To explore the regulatory role of miR-1908 in renal fibrosis.
  
  
  METHODS
  The level of miR-1908 and transforming growth factor beta 1 (TGF-β1) mRNA during renal fibrosis were detected with real-time quantitative PCR. Bioinformatics and luciferase reporter gene analyses were applied to determine the targeting relationship between miR-1908 and TGF-β1 mRNA. After primary human renal interstitial fibroblasts were transfected with miR-1908 adenoviral expression vector in vitro, Western blotting was used to detect the protein levels of TGF-β1, smad2/3 and matrix metalloproteinase 2 (MMP-2) in the cells. Six weeks after intraperitoneal injection of miR-1908 adenoviral vector, the renal tissue sections of the renal fibrosis mouse models were stained with Masson staining.
  
  
  RESULTS
  Human miR-1908 showed a gradually decreasing expression during renal fibrosis process, which was completely contrary to the changes of TGF-β1 mRNA. Overexpression of miR-1908 suppressed the expressions of TGF-β1, smad2/3 and MMP-2 in human primary renal interstitial cells. The renal fibrosis was significantly relieved in the mice injected with miR-1908 adenovirus vector injection compared with the ones without injection.
  
  
  CONCLUSIONS
  miR-1908 could inhibit renal fibrosis through targeting TGF-β1.
authors.affiliation: Department of Nephrology, Shaanxi General Hospital of CAPF, Xi' an 710054, China. *Corresponding author, E-mail: xiefeixf@sohu.com.
authors.forename: Fei
authors.initials: F
authors.lastname: Xie
authors.affiliation: Department of Nephrology, Shaanxi General Hospital of CAPF, Xi' an 710054, China.
authors.forename: Xiaoshun
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Nephrology, Shaanxi General Hospital of CAPF, Xi' an 710054, China.
authors.forename: Chan
authors.initials: C
authors.lastname: Wei
authors.affiliation: Department of Nephrology, Shaanxi General Hospital of CAPF, Xi' an 710054, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Gou
authors.affiliation: Department of Nephrology, Shaanxi General Hospital of CAPF, Xi' an 710054, China.
authors.forename: Yanlong
authors.initials: Y
authors.lastname: Dang
authors.affiliation: Department of Nephrology, Shaanxi General Hospital of CAPF, Xi' an 710054, China.
authors.forename: Zelei
authors.initials: Z
authors.lastname: Shan
mesh_terms: D000328:Adult; D000818:Animals; D015536:Down-Regulation; D005260:Female; D005355:Fibrosis / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007668:Kidney / Q000378:metabolism / Q000473:pathology; D007674:Kidney Diseases / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism; D051379:Mice; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D015398:Signal Transduction; D051899:Smad2 Protein / Q000235:genetics / Q000378:metabolism; D053773:Transforming Growth Factor beta1 / Q000235:genetics* / Q000378:metabolism
chemical_list: C577645:MIRN1908 microRNA, human; D035683:MicroRNAs; C497782:SMAD2 protein, human; D051899:Smad2 Protein; D053773:Transforming Growth Factor beta1; D020778:Matrix Metalloproteinase 2