title: Combinatorial application of two aldehyde oxidoreductases on isobutanol production in the presence of furfural.
abstract: Furfural is a toxic by-product formulated from pretreatment processes of lignocellulosic biomass. In order to utilize the lignocellulosic biomass on isobutanol production, inhibitory effect of the furfural on isobutanol production was investigated and combinatorial application of two oxidoreductases, FucO and YqhD, was suggested as an alternative strategy. Furfural decreased cell growth and isobutanol production when only YqhD or FucO was employed as an isobutyraldehyde oxidoreductase. However, combinatorial overexpression of FucO and YqhD could overcome the inhibitory effect of furfural giving higher isobutanol production by 110% compared with overexpression of YqhD. The combinatorial oxidoreductases increased furfural detoxification rate 2.1-fold and also accelerated glucose consumption 1.4-fold. When it compares to another known system increasing furfural tolerance, membrane-bound transhydrogenase (pntAB), the combinatorial aldehyde oxidoreductases were better on cell growth and production. Thus, to control oxidoreductases is important to produce isobutanol using furfural-containing biomass and the combinatorial overexpression of FucO and YqhD can be an alternative strategy.
authors.affiliation: Department of Microbial Engineering, College of Engineering, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul, 143-701, Republic of Korea.
authors.forename: Hyung-Min
authors.initials: HM
authors.lastname: Seo
authors.affiliation: Department of Microbial Engineering, College of Engineering, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul, 143-701, Republic of Korea.
authors.forename: Jong-Min
authors.initials: JM
authors.lastname: Jeon
authors.affiliation: Department of Microbial Engineering, College of Engineering, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul, 143-701, Republic of Korea.
authors.forename: Ju Hee
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of Microbial Engineering, College of Engineering, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul, 143-701, Republic of Korea.
authors.forename: Hun-Suk
authors.initials: HS
authors.lastname: Song
authors.affiliation: Department of Microbial Engineering, College of Engineering, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul, 143-701, Republic of Korea.
authors.forename: Han-Byul
authors.initials: HB
authors.lastname: Joo
authors.affiliation: Food Ingredients Center, Foods R&D, CheilJedang, Guro-dong, Guro-gu, Seoul, 152-051, Republic of Korea.
authors.forename: Sung-Hee
authors.initials: SH
authors.lastname: Park
authors.affiliation: Department of Environmental Engineering, Ajou University, 206, World Cup-ro, Yeongtong-gu, Suwon, Gyeonggi-do, 443-749, Republic of Korea.
authors.forename: Kwon-Young
authors.initials: KY
authors.lastname: Choi
authors.affiliation: Biotechnology Process Engineering Center, Korea Research Institute Bioscience Biotechnology (KRIBB), Gwahangno, Yuseong-gu, Taejon, 305-806, Republic of Korea.
authors.forename: Yong Hyun
authors.initials: YH
authors.lastname: Kim
authors.affiliation: Department of Biological and Chemical Engineering, Hongik University, Sejong-ro 2639, Jochiwon, Sejong City, Republic of Korea.
authors.forename: Kyungmoon
authors.initials: K
authors.lastname: Park
authors.affiliation: Biotechnology Process Engineering Center, Korea Research Institute Bioscience Biotechnology (KRIBB), Gwahangno, Yuseong-gu, Taejon, 305-806, Republic of Korea.
authors.forename: Jungoh
authors.initials: J
authors.lastname: Ahn
authors.affiliation: Biotechnology Process Engineering Center, Korea Research Institute Bioscience Biotechnology (KRIBB), Gwahangno, Yuseong-gu, Taejon, 305-806, Republic of Korea.
authors.forename: Hongweon
authors.initials: H
authors.lastname: Lee
authors.affiliation: Department of Microbial Engineering, College of Engineering, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul, 143-701, Republic of Korea. seokor@konkuk.ac.kr.|Institute for Ubiquitous Information Technology and Applications (CBRU), Konkuk University, Seoul, 143-701, Republic of Korea. seokor@konkuk.ac.kr.
authors.forename: Yung-Hun
authors.initials: YH
authors.lastname: Yang
mesh_terms: D000445:Aldehyde Oxidoreductases / Q000378:metabolism*; D000447:Aldehydes / Q000378:metabolism; D018533:Biomass; D000440:Butanols / Q000378:metabolism*; D002455:Cell Division / Q000187:drug effects; D004926:Escherichia coli / Q000166:cytology / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D005662:Furaldehyde / Q000378:metabolism* / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism; D009250:NADP Transhydrogenases / Q000378:metabolism
chemical_list: D000447:Aldehydes; D000440:Butanols; C040507:isobutyl alcohol; C017439:isobutyraldehyde; D005662:Furaldehyde; D000445:Aldehyde Oxidoreductases; D009250:NADP Transhydrogenases; D005947:Glucose
keywords: Aldehyde oxidoreductase; Escherichia coli; Furfural; Isobutanol