title: Complete mitochondrial genome of Skeletonema marinoi (Mediophyceae, Bacillariophyta), a clonal chain forming diatom in the west coast of Korea.
abstract: The complete mitochondrial DNA of common planktonic diatom, Skeletonema marinoi JK029 was sequenced and characterized. The circular mitogenome contains 62 genes in 38 515 bp (29.7% GC), including 35 protein-coding, 2 rRNA, and 25 tRNA genes. Total 80% of protein-coding genes have usual ATG start codon and 20% have alternative start codons. The GC content of tRNA genes (39.8%) is relatively higher than those of the rRNA (32.9%) and CDS (29.3%). There are four cases of gene overlapping between neighboring genes, i.e., rrs-trnM, rps2-rps4, nad1-tatC, and rps11-trnY. Newly determined mitogenome of S. marinoi was compared with available seven diatoms and eight stramenopiles by using the maximum-likelihood analysis. The 34-CDS concatenated data (8528 amino acids) support the monophyly of Bacillariophyta. However, mitogenome data showed different higher class-levels clustering with previous study. These results suggested that additional mitogenome data will provide useful information for mitochondrial genome diversity and evolution of the diatoms and stramenopiles.
authors.affiliation: a Marine Ecosystem and Biological Research Center, Korea Institute of Ocean Science & Technology , Ansan , Republic of Korea.
authors.forename: Sung Min
authors.initials: SM
authors.lastname: An
authors.affiliation: b Department of Marine Sciences and Convergence Technology , Hanyang University , Ansan , Republic of Korea , and.
authors.forename: Soo Yeon
authors.initials: SY
authors.lastname: Kim
authors.affiliation: a Marine Ecosystem and Biological Research Center, Korea Institute of Ocean Science & Technology , Ansan , Republic of Korea.
authors.forename: Jae Hoon
authors.initials: JH
authors.lastname: Noh
authors.affiliation: a Marine Ecosystem and Biological Research Center, Korea Institute of Ocean Science & Technology , Ansan , Republic of Korea.|c Department of Marine Biology , Korea University of Science and Technology , Daejeon , Republic of Korea.
authors.forename: Eun Chan
authors.initials: EC
authors.lastname: Yang
mesh_terms: D001482:Base Composition; D018387:Codon, Initiator; D018388:Codon, Terminator; D004272:DNA, Mitochondrial / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D017377:Diatoms / Q000145:classification / Q000235:genetics*; D054629:Genome, Mitochondrial; D010802:Phylogeny; D012335:RNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D012343:RNA, Transfer / Q000737:chemistry / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA
chemical_list: D018387:Codon, Initiator; D018388:Codon, Terminator; D004272:DNA, Mitochondrial; D012335:RNA, Ribosomal; D012343:RNA, Transfer
keywords: Bacillariophyceae; Skeletonema marinoi JK029; diatoms; mitochondrial genome; stramenopiles