title: Matrix Metalloproteinase-9/Neutrophil Gelatinase-Associated Lipocalin Complex Activity in Human Glioma Samples Predicts Tumor Presence and Clinical Prognosis.
abstract: Matrix metalloproteinase-9/neutrophil gelatinase-associated lipocalin (MMP-9/NGAL) complex activity is elevated in brain tumors and may serve as a molecular marker for brain tumors. However, the relationship between MMP-9/NGAL activity in brain tumors and patient prognosis and treatment response remains unclear. Here, we compared the clinical characteristics of glioma patients with the MMP-9/NGAL activity measured in their respective tumor and urine samples. Using gelatin zymography assays, we found that MMP-9/NGAL activity was significantly increased in tumor tissues (TT) and preoperative urine samples (Preop-1d urine). Activity was reduced by seven days after surgery (Postop-1w urine) and elevated again in cases of tumor recurrence. The MMP-9/NGAL status correlated well with MRI-based tumor assessments. These findings suggest that MMP-9/NGAL activity could be a novel marker to detect gliomas and predict the clinical outcome of patients.
authors.affiliation: Department of Neurosurgery, Affiliated Shantou Hospital of Sun Yat-sen University, Shantou 515041, China.
authors.forename: Ming-Fa
authors.initials: MF
authors.lastname: Liu
authors.affiliation: Department of Neurosurgery, Affiliated Shantou Hospital of Sun Yat-sen University, Shantou 515041, China.
authors.forename: Yong-Yang
authors.initials: YY
authors.lastname: Hu
authors.affiliation: Department of Neurosurgery, Affiliated Shantou Hospital of Sun Yat-sen University, Shantou 515041, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Jin
authors.affiliation: Department of Neurosurgery, Affiliated Shantou Hospital of Sun Yat-sen University, Shantou 515041, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Xu
authors.affiliation: Department of Pathology, Affiliated Shantou Hospital of Sun Yat-sen University, Shantou 515041, China.
authors.forename: Shao-Hong
authors.initials: SH
authors.lastname: Wang
authors.affiliation: Department of Radiology, Affiliated Shantou Hospital of Sun Yat-sen University, Shantou 515041, China.
authors.forename: Guang-Zhou
authors.initials: GZ
authors.lastname: Du
authors.affiliation: Department of Biochemistry and Molecular Biology, Shantou University Medical College, Shantou 515041, China.
authors.forename: Bing-Li
authors.initials: BL
authors.lastname: Wu
authors.affiliation: Institute of Oncologic Pathology, Shantou University Medical College, Shantou 515041, China.
authors.forename: Li-Yan
authors.initials: LY
authors.lastname: Li
authors.affiliation: Institute of Oncologic Pathology, Shantou University Medical College, Shantou 515041, China.
authors.forename: Li-Yan
authors.initials: LY
authors.lastname: Xu
authors.affiliation: Department of Biochemistry and Molecular Biology, Shantou University Medical College, Shantou 515041, China.
authors.forename: En-Min
authors.initials: EM
authors.lastname: Li
authors.affiliation: Department of Neurosurgery, Affiliated Shantou Hospital of Sun Yat-sen University, Shantou 515041, China.
authors.forename: Hai-Xiong
authors.initials: HX
authors.lastname: Xu
mesh_terms: D000209:Acute-Phase Proteins / Q000235:genetics / Q000378:metabolism* / Q000652:urine; D000293:Adolescent; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism* / Q000652:urine; D001932:Brain Neoplasms / Q000175:diagnosis*; D016022:Case-Control Studies; D002648:Child; D005260:Female; D005910:Glioma / Q000175:diagnosis*; D006801:Humans; D000071068:Lipocalin-2; D054834:Lipocalins / Q000235:genetics / Q000378:metabolism* / Q000652:urine; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism* / Q000652:urine; D008875:Middle Aged; D011379:Prognosis; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism* / Q000652:urine
chemical_list: D000209:Acute-Phase Proteins; D014408:Biomarkers, Tumor; C489506:LCN2 protein, human; D000071068:Lipocalin-2; D054834:Lipocalins; D011518:Proto-Oncogene Proteins; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9