title: [Effect of hydrogen-saline on lung injury and heme oxygenase-1 expression in the lung tissue of acute paraquat-intoxicated mice].
abstract: OBJECTIVE
  To investigate the effect of Hydrogen-saline on Lung Injury and HO-1 Expression in The Lung Tissue of Acute Paraquat-intoxicated mice.
  
  
  METHODS
  108 male clean-grade mice were divided randomly into 3 groups: normal control group (n = 8), PQ group (n = 50) and PQ HN group (n = 50), PQ group were lavaged with 20% PQ (50 mg/kg). PQ+Hyclrogen saline group were intxaperitoneal injected with 5 ml/kg saturated hydragen saline after lavaged, 2 times/d. We observe its performance after the poisoning, The lung tissue were taken to HE stained, MDA and SOD activity of lung tissue homogenate were detected, HO-1 activity were observed by immunohistochemistry and western method at 6 h, 1 d, 3 d, 7 d and 14 d.
  
  
  RESULTS
  mice came to shortness of breath, exhaustion and death after poisoned. PQ+hydrogen saline group was more alleviative than PQ group. The lung MDA of PQ group was markedly increased at 6 h and 24 h and SOD was decreased at 6 h, 24 h, 3 d, 7 d, 14 d than normal control group. In Comparison with PQ group, the lung MDA was decreased at 24 h and SOD was increased at 24 h and 3 d (P < 0.05). HO-1 expression trend and distribution in PQ+hydrogen saline group are similar with PQ group, but were significantly higher than that of PQ group and the control group each time point (P < 0.05).
  
  
  CONCLUSIONS
  Oxidative stress plays important roles in lung injury caused by paraquat. Hydrogen-saline may increase expression of HO-1 and alleviate oxidative stress damage in lung.
authors.affiliation: Emergency Department of University-Town Hospital of Chongqing Medical University, Chongqing 401331, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Liu
authors.forename: Dongmei
authors.initials: D
authors.lastname: Song
authors.affiliation: E-mail: 565497171@qq.com.
authors.forename: Yu
authors.initials: Y
authors.lastname: Jiang
authors.forename: Yun
authors.initials: Y
authors.lastname: Ge
mesh_terms: D055371:Acute Lung Injury / Q000378:metabolism / Q000473:pathology*; D000818:Animals; D051547:Heme Oxygenase-1 / Q000378:metabolism*; D006859:Hydrogen; D008168:Lung / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D018384:Oxidative Stress; D010269:Paraquat / Q000633:toxicity*; D012462:Saline Solution, Hypertonic / Q000627:therapeutic use*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D008565:Membrane Proteins; D012462:Saline Solution, Hypertonic; D008315:Malondialdehyde; D006859:Hydrogen; D051547:Heme Oxygenase-1; C496542:Hmox1 protein, mouse; D013482:Superoxide Dismutase; D010269:Paraquat