title: Colon Cancer Chemoprevention by Sage Tea Drinking: Decreased DNA Damage and Cell Proliferation.
abstract: Salvia officinalis and some of its isolated compounds have been found to be preventive of DNA damage and increased proliferation in vitro in colon cells. In the present study, we used the azoxymethane model to test effects of S. officinalis on colon cancer prevention in vivo. The results showed that sage treatment reduced the number of ACF formed only if administered before azoxymethane injection, demonstrating that sage tea drinking has a chemopreventive effect on colorectal cancer. A decrease in the proliferation marker Ki67 and in H2 O2 -induced and azoxymethane-induced DNA damage to colonocytes and lymphocytes were found with sage treatment. This confirms in vivo the chemopreventive effects of S. officinalis. Taken together, our results show that sage treatment prevented initiation phases of colon carcinogenesis, an effect due, at least in part, to DNA protection, and reduced proliferation rates of colon epithelial cell that prevent mutations and their fixation through cell replication. These chemopreventive effects of S. officinalis on colon cancer add to the many health benefits attributed to sage and encourage its consumption.
authors.affiliation: Department of Biology, School of Sciences, University of Minho, 4710-057, Braga, Portugal.
authors.forename: Dalila F N
authors.initials: DF
authors.lastname: Pedro
authors.affiliation: Department of Biology, School of Sciences, University of Minho, 4710-057, Braga, Portugal.|Laboratory of Cellular, Molecular and Analytical Studies, CIIMAR, University of Porto, 4050-123, Porto, Portugal (Present adress).
authors.forename: Alice A
authors.initials: AA
authors.lastname: Ramos
authors.affiliation: CITAB, Department of Biology, School of Sciences, University of Minho, 4710-057, Braga, Portugal.
authors.forename: Cristovao F
authors.initials: CF
authors.lastname: Lima
authors.affiliation: Life and Health Sciences Research Institute (ICVS), Health Sciences School, University of Minho, 4710-057, Braga, Portugal.|ICVS/3B's - PT Government Associate Laboratory, Braga/Guimarães, Portugal.
authors.forename: Fatima
authors.initials: F
authors.lastname: Baltazar
authors.affiliation: CITAB, Department of Biology, School of Sciences, University of Minho, 4710-057, Braga, Portugal.
authors.forename: Cristina
authors.initials: C
authors.lastname: Pereira-Wilson
mesh_terms: D058739:Aberrant Crypt Foci / Q000473:pathology; D000818:Animals; D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D001397:Azoxymethane; D049109:Cell Proliferation / Q000187:drug effects; D018890:Chemoprevention; D015179:Colorectal Neoplasms / Q000517:prevention & control*; D019392:Cytochrome P-450 CYP2E1 / Q000378:metabolism; D004249:DNA Damage / Q000187:drug effects*; D005260:Female; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D011916:Rats, Inbred F344; D027543:Salvia officinalis / Q000737:chemistry*; D000069459:Teas, Herbal
chemical_list: D016588:Anticarcinogenic Agents; D010936:Plant Extracts; D000069459:Teas, Herbal; D019392:Cytochrome P-450 CYP2E1; D001397:Azoxymethane
keywords: DNA damage; Salvia officinalis tea; colon cancer; comet assay