title: Rapid and reliable genotyping of HLA-B*58:01 in four Chinese populations using a single-tube duplex real-time PCR assay.
abstract: OBJECTIVE
  HLA-B*58:01 is strongly associated with allopurinol-induced severe cutaneous adverse reactions. This study aimed to develop a new and convenient method for HLA-B*58:01 genotyping and to investigate HLA-B*58:01 distribution in different Chinese populations.
  
  
  METHODS
  Combining of sequence-specific primers and TaqMan probe, a single-tube duplex real-time PCR assay for HLA-B*58:01 typing was established.
  
  
  RESULTS
  The HLA-B*58:01 genotyping result in Buyei (n = 100) by real-time PCR showed 100% concordance with those by sequence-based typing. The prevalence of HLA-B*58:01 carrier in Buyei (17%, n = 100) was significantly higher than those in Northern Han (4%, n = 100), Tibetan (5.1%, n = 99) and Uighur (2%, n = 50) populations (p < 0.05).
  
  
  CONCLUSIONS
  The newly developed reliable assay was appropriate for HLA-B*58:01 detection prior to allopurinol administration in clinical settings.
authors.affiliation: The National Engineering Research Center for Miniaturized Detection Systems; College of Life Science, Northwest University, Xi'an, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Kang
authors.affiliation: The National Engineering Research Center for Miniaturized Detection Systems; College of Life Science, Northwest University, Xi'an, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Chen
authors.affiliation: The National Engineering Research Center for Miniaturized Detection Systems; College of Life Science, Northwest University, Xi'an, China.
authors.forename: Min
authors.initials: M
authors.lastname: Han
authors.affiliation: The National Engineering Research Center for Miniaturized Detection Systems; College of Life Science, Northwest University, Xi'an, China.
authors.forename: Zhengbin
authors.initials: Z
authors.lastname: Liu
authors.affiliation: The National Engineering Research Center for Miniaturized Detection Systems; College of Life Science, Northwest University, Xi'an, China.
authors.forename: Jinhui
authors.initials: J
authors.lastname: Liu
authors.affiliation: The National Engineering Research Center for Miniaturized Detection Systems; College of Life Science, Northwest University, Xi'an, China.
authors.forename: Penggao
authors.initials: P
authors.lastname: Dai
authors.affiliation: The National Engineering Research Center for Miniaturized Detection Systems; College of Life Science, Northwest University, Xi'an, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Chen
authors.affiliation: The National Engineering Research Center for Miniaturized Detection Systems; College of Life Science, Northwest University, Xi'an, China.
authors.forename: Huijuan
authors.initials: H
authors.lastname: Wang
mesh_terms: D000493:Allopurinol / Q000009:adverse effects; D044466:Asian People; D005838:Genotype; D015235:HLA-B Antigens / Q000235:genetics*; D006801:Humans; D015810:Linkage Disequilibrium; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D014969:Xanthine Oxidase / Q000037:antagonists & inhibitors
chemical_list: D015235:HLA-B Antigens; C041533:HLA-B58 antigen; D000493:Allopurinol; D014969:Xanthine Oxidase
keywords: HLA-B*58:01; allopurinol; real-time PCR; severe cutaneous adverse reactions