title: In Planta Response of Arabidopsis to Photothermal Impact Mediated by Gold Nanoparticles.
abstract: Biological responses to photothermal effects of gold nanoparticles (GNPs) have been demonstrated and employed for various applications in diverse systems except for one important class - plants. Here, the uptake of GNPs through Arabidopsis thaliana roots and translocation to leaves are reported. Successful plasmonic nanobubble generation and acoustic signal detection in planta is demonstrated. Furthermore, Arabidopsis leaves harboring GNPs and exposed to continuous laser or noncoherent light show elevated temperatures across the leaf surface and induced expression of heat-shock regulated genes. Overall, these results demonstrate that Arabidopsis can readily take up GNPs through the roots and translocate the particles to leaf tissues. Once within leaves, GNPs can act as photothermal agents for on-demand remote activation of localized biological processes in plants.
authors.affiliation: Department of BioSciences, Rice University, Houston, TX, 77005, USA.
authors.forename: Yeonjong
authors.initials: Y
authors.lastname: Koo
authors.affiliation: Department of BioSciences, Rice University, Houston, TX, 77005, USA.
authors.forename: Ekaterina Y
authors.initials: EY
authors.lastname: Lukianova-Hleb
authors.affiliation: Department of BioSciences, Rice University, Houston, TX, 77005, USA.
authors.forename: Joann
authors.initials: J
authors.lastname: Pan
authors.affiliation: Department of Horticultural Sciences, Texas A&M University, College Station, TX, 77843, USA.
authors.forename: Sean M
authors.initials: SM
authors.lastname: Thompson
authors.affiliation: Department of BioSciences, Rice University, Houston, TX, 77005, USA.|Department of Physics and Astronomy, Rice University, Houston, TX, 77005, USA.
authors.forename: Dmitri O
authors.initials: DO
authors.lastname: Lapotko
authors.affiliation: Department of BioSciences, Rice University, Houston, TX, 77005, USA.
authors.forename: Janet
authors.initials: J
authors.lastname: Braam
mesh_terms: D000162:Acoustics; D017360:Arabidopsis / Q000187:drug effects* / Q000528:radiation effects*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D001692:Biological Transport / Q000187:drug effects / Q000528:radiation effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000528:radiation effects; D017343:Genes, Plant; D006046:Gold / Q000494:pharmacology*; D021621:Imaging, Three-Dimensional; D007834:Lasers; D008027:Light; D053768:Metal Nanoparticles / Q000737:chemistry*; D018515:Plant Leaves / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D058526:Plant Vascular Bundle / Q000187:drug effects / Q000528:radiation effects; D013696:Temperature
chemical_list: D029681:Arabidopsis Proteins; D006046:Gold