title: Urinary apolipoprotein M as a biomarker of acute kidney injury in children undergoing heart surgery.
abstract: OBJECTIVE
  To investigate whether apoM is excreted in urine of children undergoing heart surgery and the potential of apoM as early biomarker of acute kidney injury (AKI).
  
  
  METHODS
  Urine was collected in children undergoing heart surgery. ApoM was measured with ELISA. U-apoM was characterized by gel filtration chromatography and western blotting.
  
  
  RESULTS
  ApoM was excreted into the urine 0-4 h postoperatively as the full-length apoM in particles smaller than plasma HDL. At 0 h, U-apoM predicted AKI with an area under the receiver-operating characteristics curve of 0.70 (p < 0.018). Sensitivity was 0.71 and specificity was 0.68 at a cutoff level at 1.45 nmol/l.
  
  
  CONCLUSIONS
  ApoM is excreted in the urine of children after cardiac surgery. Its potential as biomarker of AKI deserves exploration.
authors.affiliation: Department of Clinical Biochemistry, Rigshospitalet, University of Copenhagen, Denmark.
authors.forename: Eva Martha Madsen
authors.initials: EM
authors.lastname: Svarrer
authors.affiliation: Department of Cardiothoracic Surgery, Rigshospitalet, University of Copenhagen, Denmark.
authors.forename: Henrik Ørbæk
authors.initials: HØ
authors.lastname: Andersen
authors.affiliation: Department of Cardiothoracic Surgery, Rigshospitalet, University of Copenhagen, Denmark.
authors.forename: Morten
authors.initials: M
authors.lastname: Helvind
authors.affiliation: Department of Nephrology, University of Groningen & University Medical Center Groningen, Holland.
authors.forename: Maartje C J
authors.initials: MC
authors.lastname: Slagman
authors.affiliation: Department of Nephrology, University of Groningen & University Medical Center Groningen, Holland.
authors.forename: Gerjan
authors.initials: G
authors.lastname: Navis
authors.affiliation: Department of Endocrinology, University of Groningen & University Medical Center Groningen, Holland.
authors.forename: Robin P F
authors.initials: RP
authors.lastname: Dullaart
authors.affiliation: Department of Laboratory Medicine, Division of Clinical Chemistry, Lund University, University Hospital, Malmö, Sweden.
authors.forename: Björn
authors.initials: B
authors.lastname: Dahlbäck
authors.affiliation: Department of Clinical Biochemistry, Rigshospitalet, University of Copenhagen, Denmark.|Department of Biomedical Sciences, University of Copenhagen, Copenhagen, Denmark.|Department of Clinical Medicine, University of Copenhagen, Copenhagen, Denmark.
authors.forename: Lars Bo
authors.initials: LB
authors.lastname: Nielsen
mesh_terms: D058186:Acute Kidney Injury / Q000601:surgery* / Q000652:urine*; D000209:Acute-Phase Proteins / Q000652:urine; D001053:Apolipoproteins / Q000652:urine*; D000075243:Apolipoproteins M; D015415:Biomarkers / Q000652:urine; D006348:Cardiac Surgical Procedures; D002648:Child; D002675:Child, Preschool; D015331:Cohort Studies; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D000071068:Lipocalin-2; D054834:Lipocalins / Q000652:urine*; D008297:Male; D011518:Proto-Oncogene Proteins / Q000652:urine
chemical_list: C400523:APOM protein, human; D000209:Acute-Phase Proteins; D001053:Apolipoproteins; D000075243:Apolipoproteins M; D015415:Biomarkers; C489506:LCN2 protein, human; D000071068:Lipocalin-2; D054834:Lipocalins; D011518:Proto-Oncogene Proteins
keywords: AKI; Apolipoprotein M; acute kidney injury; apoM; biomarker; cardiac surgery; urine