title: Proteomic analysis of physiological versus pathological cardiac remodeling in animal models expressing mutations in myosin essential light chains.
abstract: In this study we aimed to provide an in-depth proteomic analysis of differentially expressed proteins in the hearts of transgenic mouse models of pathological and physiological cardiac hypertrophy using tandem mass tag labeling and liquid chromatography tandem mass spectrometry. The Δ43 mouse model, expressing the 43-amino-acid N-terminally truncated myosin essential light chain (ELC) served as a tool to study the mechanisms of physiological cardiac remodeling, while the pathological hypertrophy was investigated in A57G (Alanine 57 → Glycine) ELC mice. The results showed that 30 proteins were differentially expressed in Δ43 versus A57G hearts as determined by multiple pair comparisons of the mutant versus wild-type (WT) samples with P < 0.05. The A57G hearts showed differential expression of nine mitochondrial proteins involved in metabolic processes compared to four proteins for ∆43 hearts when both mutants were compared to WT hearts. Comparisons between ∆43 and A57G hearts showed an upregulation of three metabolically important mitochondrial proteins but downregulation of nine proteins in ∆43 hearts. The physiological model of cardiac hypertrophy (∆43) showed no changes in the levels of Ca(2+)-binding proteins relative to WT, while the pathologic model (A57G) showed the upregulation of three Ca(2+)-binding proteins, including sarcalumenin. Unique differences in chaperone and fatty acid metabolism proteins were also observed in Δ43 versus A57G hearts. The proteomics data support the results from functional studies performed previously on both animal models of cardiac hypertrophy and suggest that the A57G- and not ∆43- mediated alterations in fatty acid metabolism and Ca(2+) homeostasis may contribute to pathological cardiac remodeling in A57G hearts.
authors.affiliation: Department of Neurobiology, Physiology, and Behavior, University of California, Davis, CA, 95616, USA. avgomes@ucdavis.edu.
authors.forename: Aldrin V
authors.initials: AV
authors.lastname: Gomes
authors.affiliation: Department of Molecular and Cellular Pharmacology, University of Miami Miller School of Medicine, Miami, FL, 33136, USA.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Kazmierczak
authors.affiliation: Department of Neurobiology, Physiology, and Behavior, University of California, Davis, CA, 95616, USA.
authors.forename: Jenice X
authors.initials: JX
authors.lastname: Cheah
authors.affiliation: Department of Neurobiology, Physiology, and Behavior, University of California, Davis, CA, 95616, USA.
authors.forename: Jennifer E
authors.initials: JE
authors.lastname: Gilda
authors.affiliation: Department of Molecular and Cellular Pharmacology, University of Miami Miller School of Medicine, Miami, FL, 33136, USA.
authors.forename: Chen-Ching
authors.initials: CC
authors.lastname: Yuan
authors.affiliation: Department of Molecular and Cellular Pharmacology, University of Miami Miller School of Medicine, Miami, FL, 33136, USA.
authors.forename: Zhiqun
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: Department of Molecular and Cellular Pharmacology, University of Miami Miller School of Medicine, Miami, FL, 33136, USA. dszczesna@med.miami.edu.
authors.forename: Danuta
authors.initials: D
authors.lastname: Szczesna-Cordary
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D006332:Cardiomegaly / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D004195:Disease Models, Animal; D015536:Down-Regulation / Q000502:physiology; D005260:Female; D006321:Heart / Q000502:physiology*; D051379:Mice; D008822:Mice, Transgenic; D024101:Mitochondrial Proteins / Q000378:metabolism; D009154:Mutation / Q000235:genetics*; D009206:Myocardium / Q000378:metabolism*; D018994:Myosin Light Chains / Q000235:genetics* / Q000378:metabolism; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D015854:Up-Regulation / Q000502:physiology; D020257:Ventricular Remodeling / Q000235:genetics / Q000502:physiology*
chemical_list: D024101:Mitochondrial Proteins; D018994:Myosin Light Chains; D020543:Proteome; D002118:Calcium
keywords: Molecular proteomics; Myosin ELC; Pathological hypertrophy; Transgenic mice