title: Electrochemical biosensor for Mycobacterium tuberculosis DNA detection based on gold nanotubes array electrode platform.
abstract: The template assisted electrochemical deposition technique was used for the synthesis of gold nanotubes array (AuNTsA). The morphological structure of the synthesized AuNTsA was observed by scanning electron microscopy and found that the individual nanotubes are around 1.5 μm in length with a diameter of 200 nm. Nanotubes are vertically aligned to the Au thick film, which is formed during the synthesis process of nanotubes. The electrochemical performance of the AuNTsA was compared with the bare Au electrode and found that AuNTsA has better electron transfer surface than bare Au electrode which is due to the high surface area. Hence, the AuNTsA was used as an electrode for the fabrication of DNA hybridization biosensor for detection of Mycobacterium Tuberculosis DNA. The DNA hybridization biosensor constructed by AuNTsA electrode was characterized by cyclic voltammetry technique with Fe(CN)6(3-/4-) as an electrochemical redox indicator. The selectivity of the fabricated biosensor was illustrated by hybridization with complementary DNA and non-complementary DNA with probe DNA immobilized AuNTsA electrode using methylene blue as a hybridization indicator. The developed electrochemical DNA biosensor shows good linear range of complementary DNA concentration from 0.01 ng/μL to 100 ng/μL with high detection limit.
authors.affiliation: Department of Emerging Materials Science, DGIST, Daegu 711-873, Republic of Korea.
authors.forename: Sri Ramulu
authors.initials: SR
authors.lastname: Torati
authors.affiliation: Department of Emerging Materials Science, DGIST, Daegu 711-873, Republic of Korea; School of Engineering Science and Technology, University of Hyderabad, Gachibowli, Hyderabad 500046, India.
authors.forename: Venu
authors.initials: V
authors.lastname: Reddy
authors.affiliation: Department of Physics, Andong National University, Andong 760-749, Korea.
authors.forename: Seok Soo
authors.initials: SS
authors.lastname: Yoon
authors.affiliation: Department of Emerging Materials Science, DGIST, Daegu 711-873, Republic of Korea. Electronic address: cgkim@dgist.ac.kr.
authors.forename: CheolGi
authors.initials: C
authors.lastname: Kim
mesh_terms: D015374:Biosensing Techniques; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification*; D055664:Electrochemical Techniques; D006046:Gold / Q000737:chemistry; D006801:Humans; D057230:Limit of Detection; D008855:Microscopy, Electron, Scanning; D009169:Mycobacterium tuberculosis / Q000737:chemistry / Q000302:isolation & purification* / Q000472:pathogenicity; D043942:Nanotubes / Q000737:chemistry; D014376:Tuberculosis / Q000175:diagnosis* / Q000382:microbiology
chemical_list: D004269:DNA, Bacterial; D006046:Gold
keywords: DNA biosensor; Electrodeposition; Hybridization; Methylene blue; Nanotubes