title: SNaPBceBcon: a Practical Tool for Identification and Genotyping of Burkholderia cepacia and Burkholderia contaminans.
abstract: We propose an optimized protocol for an extensive population analysis of Burkholderia cepacia and Burkholderia contaminans. Seven new polymorphisms were added to the recently proposed SNaPBcen assay, and a total of 18 markers ensured the clear identification and distinction of B. cepacia and B. contaminans isolates and high genotypic discrimination (Simpson index of 0.94) compared to those for multilocus sequence typing.
authors.affiliation: IPATIMUP, Institute of Molecular Pathology and Immunology, University of Porto, Porto, Portugal Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal ricjparaujo@yahoo.com.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Araujo
authors.affiliation: IPATIMUP, Institute of Molecular Pathology and Immunology, University of Porto, Porto, Portugal Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal Division of Hygiene and Medical Microbiology, Innsbruck Medical University, Innsbruck, Austria.
authors.forename: Rita
authors.initials: R
authors.lastname: Caramalho
authors.affiliation: iBB-Institute for Bioengineering and Biosciences, Department of Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, Lisbon, Portugal.
authors.forename: Carla
authors.initials: C
authors.lastname: Coutinho
authors.affiliation: iBB-Institute for Bioengineering and Biosciences, Department of Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, Lisbon, Portugal.
authors.forename: Isabel
authors.initials: I
authors.lastname: Sá-Correia
mesh_terms: D019121:Burkholderia Infections / Q000175:diagnosis / Q000209:etiology / Q000382:microbiology; D016956:Burkholderia cepacia / Q000145:classification* / Q000235:genetics*; D042602:Burkholderia cepacia complex / Q000145:classification / Q000235:genetics; D003550:Cystic Fibrosis / Q000150:complications; D005838:Genotype; D060005:Genotyping Techniques; D006801:Humans; D058885:Multilocus Sequence Typing; D020641:Polymorphism, Single Nucleotide