title: A Large-scale Survey of CRF55_01B from Men-Who-Have-Sex-with-Men in China: implying the Evolutionary History and Public Health Impact.
abstract: The HIV-1 epidemic among men-who-have-sex-with-men (MSM) continues to expand in China, involving the co-circulation of several different lineages of HIV-1 strains, including subtype B and CRF01_AE. This expansion has created conditions that facilitate the generation of new recombinant strains. A molecular epidemiologic survey among MSM in 11 provinces/cities around China was conducted from 2008 to 2013. Based on pol nucleotide sequences, a total of 19 strains (1.95%) belonged to the CRF55_01B were identified from 975 MSM in 7 provinces, with the prevalence range from 1.5% to 12.5%. Near full length genome (NFLG) sequences from six epidemiologically-unlinked MSM were amplified for analyzing evolutionary history, an identical genome structure composed of CRF01_AE and subtype B with four unique recombination breakpoints in the pol region were identified. Bayesian molecular clock analyses for both CRF01_AE and B segments indicated that the estimated time of the most recent common ancestors of CRF55_01B was around the year 2000. Our study found CRF55_01B has spread throughout the most provinces with high HIV-1 prevalence and highlights the importance of continual surveillance of dynamic changes in HIV-1 strains, the emergence of new recombinants, and the need for implementing effective prevention measures specifically targeting the MSM population in China.
authors.affiliation: Key Laboratory of AIDS Immunology of National Health and Family Planning Commission, Department of Laboratory Medicine, The First Affiliated Hospital of China Medical University, Shenyang, China.
authors.forename: Xiaoxu
authors.initials: X
authors.lastname: Han
authors.affiliation: Key Laboratory of AIDS Immunology of National Health and Family Planning Commission, Department of Laboratory Medicine, The First Affiliated Hospital of China Medical University, Shenyang, China.|AIDS Research Center, National Institute of Infectious Diseases, Tokyo, Japan.
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Takebe
authors.affiliation: Key Laboratory of AIDS Immunology of National Health and Family Planning Commission, Department of Laboratory Medicine, The First Affiliated Hospital of China Medical University, Shenyang, China.
authors.forename: Weiqing
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Key Laboratory of AIDS Immunology of National Health and Family Planning Commission, Department of Laboratory Medicine, The First Affiliated Hospital of China Medical University, Shenyang, China.
authors.forename: Minghui
authors.initials: M
authors.lastname: An
authors.affiliation: Key Laboratory of AIDS Immunology of National Health and Family Planning Commission, Department of Laboratory Medicine, The First Affiliated Hospital of China Medical University, Shenyang, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhao
authors.affiliation: Key Laboratory of AIDS Immunology of National Health and Family Planning Commission, Department of Laboratory Medicine, The First Affiliated Hospital of China Medical University, Shenyang, China.
authors.forename: Qinghai
authors.initials: Q
authors.lastname: Hu
authors.affiliation: Key Laboratory of AIDS Immunology of National Health and Family Planning Commission, Department of Laboratory Medicine, The First Affiliated Hospital of China Medical University, Shenyang, China.
authors.forename: Junjie
authors.initials: J
authors.lastname: Xu
authors.affiliation: Infectious Diseases Department, Beijing Youan Hospital, Capital Medical University, Beijing, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Wu
authors.affiliation: Sexually transmitted Disease and AIDS Department, Anhui Provincial Center for Disease Control and Prevention, Hefei, China.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Wu
authors.affiliation: Yunnan Provincial Center for Disease Control and Prevention, Kunming, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Lu
authors.affiliation: AIDS/STIs Prevention and Control Department, Hunan Provincial Center for Disease Control and Prevention, Changsha, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.affiliation: Sichuan provincial center for disease control and prevention, Chengdu, China.
authors.forename: Shu
authors.initials: S
authors.lastname: Liang
authors.affiliation: Henan Provincial Center for Disease Control and Prevention, Zhengzhou, China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Sexually Transmitted Disease and AIDS Prevention and Control Department, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing, China.
authors.forename: Hongjing
authors.initials: H
authors.lastname: Yan
authors.affiliation: Sexually transmitted Disease and AIDS Department, Shandong Provincial Center for Disease Control and Prevention, Jinan, China.
authors.forename: Jihua
authors.initials: J
authors.lastname: Fu
authors.affiliation: Infectious Disease Department, Guangzhou No. 8 Renmin Hospital, Guangzhou, China.
authors.forename: Weiping
authors.initials: W
authors.lastname: Cai
authors.affiliation: Sexually transmitted Disease and AIDS Department, Shanghai Municipal Center for Disease Control and Prevention, Shanghai, China.
authors.forename: Minghua
authors.initials: M
authors.lastname: Zhuang
authors.affiliation: Key Laboratory of AIDS Immunology of National Health and Family Planning Commission, Department of Laboratory Medicine, The First Affiliated Hospital of China Medical University, Shenyang, China.
authors.forename: Christina
authors.initials: C
authors.lastname: Liao
authors.affiliation: Key Laboratory of AIDS Immunology of National Health and Family Planning Commission, Department of Laboratory Medicine, The First Affiliated Hospital of China Medical University, Shenyang, China.|Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Shang
mesh_terms: D000328:Adult; D044466:Asian People; D002681:China / Q000453:epidemiology; D003430:Cross-Sectional Studies; D019143:Evolution, Molecular; D005787:Gene Frequency; D005838:Genotype; D005843:Geography; D015658:HIV Infections / Q000208:ethnology / Q000635:transmission / Q000821:virology*; D015497:HIV-1 / Q000145:classification / Q000235:genetics / Q000502:physiology*; D018451:Homosexuality, Male / Q000208:ethnology / Q000706:statistics & numerical data*; D054884:Host-Pathogen Interactions; D006801:Humans; D008297:Male; D010802:Phylogeny; D015995:Prevalence; D011634:Public Health / Q000706:statistics & numerical data / Q000639:trends; D013045:Species Specificity; D011795:Surveys and Questionnaires; D054302:pol Gene Products, Human Immunodeficiency Virus / Q000145:classification / Q000235:genetics
chemical_list: D054302:pol Gene Products, Human Immunodeficiency Virus