title: HDAC6 Regulates the Chaperone-Mediated Autophagy to Prevent Oxidative Damage in Injured Neurons after Experimental Spinal Cord Injury.
abstract: Hypoxia-ischemia- (HI-) induced oxidative stress plays a role in secondary pathocellular processes of acute spinal cord injury (SCI) due to HI from many kinds of mechanical trauma. Increasing evidence suggests that the histone deacetylase-6 (HDAC6) plays an important role in cell homeostasis in both physiological and abnormal, stressful, pathological conditions. This paper found that inhibition of HDAC6 accelerated reactive oxygen species (ROS) generation and cell apoptosis in response to the HI. Deficiency of HDAC6 hindered the chaperone-mediated autophagy (CMA) activity to resistance of HI-induced oxidative stress. Furthermore, this study provided the experimental evidence for the potential role of HDAC6 in the regulation of CMA by affecting HSP90 acetylation. Therefore, HDAC6 plays an important role in the function of CMA pathway under the HI stress induced by SCI and it may be a potential therapeutic target in acute SCI model.
authors.affiliation: Department of Rehabilitation, First Affiliated Hospital of Soochow University, Suzhou 215006, China ; Institute of Neuroscience, Soochow University, Suzhou 215123, China.
authors.forename: Min
authors.initials: M
authors.lastname: Su
authors.affiliation: Department of Orthopaedics, First Affiliated Hospital of Soochow University, Suzhou 215006, China.
authors.forename: Huaqing
authors.initials: H
authors.lastname: Guan
authors.affiliation: Department of Orthopaedics, First Affiliated Hospital of Soochow University, Suzhou 215006, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Institute of Neuroscience, Soochow University, Suzhou 215123, China.
authors.forename: Yarong
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Institute for Cardiovascular Science, Soochow University, Suzhou 215006, China.
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Teng
authors.affiliation: Department of Rehabilitation, First Affiliated Hospital of Soochow University, Suzhou 215006, China.
authors.forename: Weixin
authors.initials: W
authors.lastname: Yang
mesh_terms: D000818:Animals; D017209:Apoptosis; D001343:Autophagy; D004195:Disease Models, Animal; D005260:Female; D018841:HSP90 Heat-Shock Proteins / Q000378:metabolism*; D000073864:Histone Deacetylase 6; D006655:Histone Deacetylases / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D009474:Neurons / Q000378:metabolism* / Q000473:pathology; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013119:Spinal Cord Injuries / Q000378:metabolism* / Q000473:pathology
chemical_list: D018841:HSP90 Heat-Shock Proteins; D017382:Reactive Oxygen Species; C499033:Hdac6 protein, mouse; D000073864:Histone Deacetylase 6; D006655:Histone Deacetylases