title: Genome-wide association for the outcome of fixed-time artificial insemination of Brahman heifers in northern Australia.
abstract: Fixed-time AI (FTAI) is a powerful tool for genetic improvement of extensively managed beef cattle. A genomewide association study (GWAS) was conducted to investigate genes and genetic markers associated with the outcome (pregnant or not pregnant) of FTAI in 614 commercial Brahman heifers genotyped for 18,895 SNP and imputed to 51,588 SNP. The likelihood of Brahman heifers becoming pregnant after hormonal treatment to synchronize ovulation followed by FTAI was influenced by the content of their genomes, as determined by a principal component analysis. The principal component analysis involved comparisons between the studied heifers and populations of known and ancestry. The heritability of FTAI outcome was = 0.18, which is higher than for most other reproductive outcome traits. The number of SNP associated with FTAI outcome was 101 ( < 0.001, false discovery rate = 0.53). Compared with all SNP tested, associated SNP had a tendency for highly divergent allelic frequencies between and . Associated SNP were located in nearly all chromosomes, a result that shows a complex genetic architecture that is typical of highly complex traits with low heritability. Considering this and previous GWAS that examined Brahman heifer puberty and postpartum anestrus interval, 3 genomic regions emerge as important for overall Brahman heifer fertility, which mapped to chromosomes 1, 7, and 9. Further analyses, including improved genome annotation, are required to elucidate the link between these regions and heifer fertility. Additional studies are needed to confirm SNP and gene associations reported herein and further elucidate the genetics of FTAI outcome. Future GWAS should target other Braham populations and additional cattle breeds with FTAI records, including breeds with higher ancestry.
authors.forename: L R
authors.initials: LR
authors.lastname: Porto-Neto
authors.forename: S
authors.initials: S
authors.lastname: Edwards
authors.forename: M R S
authors.initials: MR
authors.lastname: Fortes
authors.forename: S A
authors.initials: SA
authors.lastname: Lehnert
authors.forename: A
authors.initials: A
authors.lastname: Reverter
authors.forename: M
authors.initials: M
authors.lastname: McGowan
mesh_terms: D000818:Animals; D002417:Cattle / Q000235:genetics* / Q000502:physiology; D005260:Female; D005298:Fertility / Q000235:genetics; D005787:Gene Frequency; D016678:Genome; D055106:Genome-Wide Association Study; D005838:Genotype; D007315:Insemination, Artificial / Q000662:veterinary*; D049590:Postpartum Period; D011247:Pregnancy; D012741:Sexual Maturation / Q000235:genetics