title: The effect of arbutin on lipid peroxidation and antioxidant capacity in the serum of cyclosporine-treated rats.
abstract: BACKGROUND
  Cyclosporine A (CsA) is a potent immunosuppressant drug with therapeutic and toxic actions. The use of CsA is limited by its toxicity. Several researchers had proposed that oxidative stress could play an important role in CsA-induced toxicity. Arbutin has recently been shown to possess antioxidative and free radical scavenging abilities. The present study was designed to investigate the in vivo effects of arbutin on lipid peroxidation and antioxidant capacity in the serum of cyclosporine treated rats.
  
  
  METHODS
  Adult male Wistar rats were divided into six groups (n=8/group): (I) control (no CsA and arbutin administration), (II and III) were treated subcutaneously (Sc) with arbutin (50,100 mg/kg/bw), respectively, (IV) administered CsA (25 mg/kg/bw) intraperitoneally (IP), (V and VI) received the combination of CsA (25 mg/kg/bw) i.p and arbutin (50,100 mg/kg/bw) Sc daily, respectively. At the end of the treatment (after3 weeks), serum lipid peroxidation was measured by thiobarbituric acid-reacting substances (TBARS) and serum total antioxidant capacity (ferric reducing ability of plasma [FRAP]) was assayed based on spectrophotometric method.
  
  
  RESULTS
  TBARS had been significantly increased by CsA administration compared with control rats. Arbutin (50mg/kg/bw) completely prevented this effect, but arbutin (100 mg/kg/bw) alone or in combination with CsA significantly increased lipid peroxidation compared with controls.
  
  
  CONCLUSIONS
  Our data indicate that arbutin (50mg/kg/bw) had protective effect in the CsA-induced toxicity but high concentration of arbutin (100mg/kg/bw) showed meaningful oxidative and lipoperoxidative effects.
authors.affiliation: Department of Biochemistry, Babol University of Medical Sciences, Babol, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Khadir
authors.affiliation: Cellular and Molecular Biology Research Center, Babol University of Medical Sciences, Babol, Iran.
authors.forename: Mahdi
authors.initials: M
authors.lastname: Pouramir
authors.affiliation: Fatemehzahra Infertility and Reproductive Health Research Center, Babol University of Medical Sciences, Babol, Iran.
authors.forename: Seyyed Gholamali
authors.initials: SG
authors.lastname: Joorsaraee
authors.affiliation: Department of Anatomy, Babol University of Medical Sciences, Babol, Iran.
authors.forename: Farideh
authors.initials: F
authors.lastname: Feizi
authors.affiliation: Non-Communicable Pediatric Diseases Research Center, Babol University of Medical Sciences, Babol, Iran.
authors.forename: Hadi
authors.initials: H
authors.lastname: Sorkhi
authors.affiliation: Department of Biochemistry, Babol University of Medical Sciences, Babol, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Yousefi
keywords: Antioxidant; Arbutin; C yclosporin A; Oxidative stress