title: Fermented Brown Rice and Rice Bran with Aspergillus oryzae (FBRA) Prevents Inflammation-Related Carcinogenesis in Mice, through Inhibition of Inflammatory Cell Infiltration.
abstract: We have established an inflammation-related carcinogenesis model in mouse, in which regressive QR-32 cells subcutaneously co-implanted with a foreign body-gelatin sponge-convert themselves into lethal tumors due to massive infiltration of inflammatory cells into the sponge. Animals were fed with a diet containing 5% or 10% fermented brown rice and rice bran with Aspergillus oryzae (FBRA). In 5% and 10% FBRA diet groups, tumor incidences were lower (35% and 20%, respectively) than in the non-treated group (70%). We found that FBRA reduced the number of inflammatory cells infiltrating into the sponge. FBRA administration did not cause myelosuppression, which indicated that the anti-inflammatory effects of FBRA took place at the inflammatory lesion. FBRA did not have antitumor effects on the implanted QRsP-11 tumor cells, which is a tumorigenic cell line established from a tumor arisen after co-implantation of QR-32 cells with sponge. FBRA did not reduce formation of 8-hydroxy-2'-deoxyguanine adducts, a marker of oxidative DNA damage in the inflammatory lesion; however, it reduced expression of inflammation-related genes such as TNF-α, Mac-1, CCL3 and CXCL2. These results suggest that FBRA will be an effective chemopreventive agent against inflammation-related carcinogenesis that acts by inhibiting inflammatory cell infiltration into inflammatory lesions.
authors.affiliation: Division of Pathological Biochemistry, Tottori University Faculty of Medicine, Tottori 683-8503, Japan. k.onuma@med.tottori-u.ac.jp.
authors.forename: Kunishige
authors.initials: K
authors.lastname: Onuma
authors.affiliation: Division of Pathological Biochemistry, Tottori University Faculty of Medicine, Tottori 683-8503, Japan. kanda@med.tottori-u.ac.jp.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Kanda
authors.affiliation: Japanese Red Cross Society, Minato-ku, Tokyo 105-8521, Japan. s04313025@yahoo.co.jp.
authors.forename: Saori
authors.initials: S
authors.lastname: Suzuki Ikeda
authors.affiliation: Division of Pathological Biochemistry, Tottori University Faculty of Medicine, Tottori 683-8503, Japan. r_sakaki1986@yahoo.co.jp.
authors.forename: Ryuta
authors.initials: R
authors.lastname: Sakaki
authors.affiliation: Division of Pathological Biochemistry, Tottori University Faculty of Medicine, Tottori 683-8503, Japan. M15M8005Z@edu.tottori-u.ac.jp.
authors.forename: Takuya
authors.initials: T
authors.lastname: Nonomura
authors.affiliation: School of Nursing and Social Services, Health Sciences University of Hokkaido, 1757 Kanazawa, Ishikari-Tobetsu, Hokkaido 061-0293, Japan. mkobaya@hoku-iryo-u.ac.jp.
authors.forename: Masanobu
authors.initials: M
authors.lastname: Kobayashi
authors.affiliation: Division of Pathological Biochemistry, Tottori University Faculty of Medicine, Tottori 683-8503, Japan. osamitsu@med.tottori-u.ac.jp.|Chromosome Engineering Research Center, Tottori University, Tottori 683-8503, Japan. osamitsu@med.tottori-u.ac.jp.
authors.forename: Mitsuhiko
authors.initials: M
authors.lastname: Osaki
authors.affiliation: Genmai Koso Co., Ltd., Sapporo, Hokkaido 001-0012, Japan. shikanai@genmaikoso.co.jp.
authors.forename: Masataka
authors.initials: M
authors.lastname: Shikanai
authors.affiliation: Sapporo Cancer Seminar Foundation, Sapporo, Hokkaido 001-0012, Japan. scs-hk@phoenix-c.or.jp.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kobayashi
authors.affiliation: Division of Pathological Biochemistry, Tottori University Faculty of Medicine, Tottori 683-8503, Japan. fuokada@med.tottori-u.ac.jp.|Chromosome Engineering Research Center, Tottori University, Tottori 683-8503, Japan. fuokada@med.tottori-u.ac.jp.
authors.forename: Futoshi
authors.initials: F
authors.lastname: Okada
mesh_terms: D000818:Animals; D001236:Aspergillus oryzae / Q000378:metabolism*; D063646:Carcinogenesis; D054405:Chemokine CCL3 / Q000235:genetics / Q000378:metabolism; D054426:Chemokine CXCL2 / Q000235:genetics / Q000378:metabolism; D004249:DNA Damage; D004032:Diet; D005260:Female; D005285:Fermentation; D007249:Inflammation / Q000517:prevention & control*; D051379:Mice; D008810:Mice, Inbred C57BL; D009369:Neoplasms / Q000517:prevention & control*; D012275:Oryza / Q000737:chemistry / Q000382:microbiology*; D018384:Oxidative Stress; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism
chemical_list: C496534:Ccl3 protein, mouse; D054405:Chemokine CCL3; D054426:Chemokine CXCL2; C404915:Cxcl2 protein, mouse; D014409:Tumor Necrosis Factor-alpha
keywords: fermented brown rice and rice bran with Aspergillus oryzae (FBRA); inflammation; inflammation-related carcinogenesis