title: Magnesium lithospermate B acts against dextran sodiumsulfate-induced ulcerative colitis by inhibiting activation of the NRLP3/ASC/Caspase-1 pathway.
abstract: This study aimed to observe the therapeutic effects of magnesium lithospermate B on acute and chronic colitis induced by dextran sodiumsulfate (DSS) and the role of inflammasome complex (NOD-like receptor protein, NLRP; apoptosis-associated speck-like protein containing, ASC; caspase-1). Establishment of acute and chronic colitis models were by using 5% DSS oral administration in BALB/C male mice. Magnesium lithospermate B (240 mg/kg body weight) was given by subcutaneous injection. Samples were collected for biomarker assay, histological examination, immunohistochemical evaluation and western blot. There was obvious increase in TNF-α level and NLPR3, ASC, and caspase-1 expressions in acute and chronic colitis groups compared with the normal control. Significant decrease of the tumor necrosis factor-α level and the expressions of NLPR3, ASC, and caspase-1 were observed after treatment with magnesium lithospermate B. This study showed that magnesium lithospermate B could be used to treat acute and chronic colitis by inhibiting the activation of the NLRP3/ASC/Caspase-1 pathway.
authors.affiliation: Department of Gastroenterology, Huashan Hospital, Fudan University, Shanghai, China.
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Jiang
authors.affiliation: Department of Gastroenterology, Huashan Hospital, Fudan University, Shanghai, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Zhong
authors.affiliation: Department of Gastroenterology, Huashan Hospital, Fudan University, Shanghai, China.
authors.forename: Dayu
authors.initials: D
authors.lastname: Sun
authors.affiliation: Department of Gastroenterology, Huashan Hospital, Fudan University, Shanghai, China. Electronic address: rlan001@126.com.
authors.forename: Lan
authors.initials: L
authors.lastname: Rong
mesh_terms: D000818:Animals; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism; D053468:CARD Signaling Adaptor Proteins; D002352:Carrier Proteins / Q000378:metabolism; D020170:Caspase 1 / Q000378:metabolism; D003093:Colitis, Ulcerative / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D016264:Dextran Sulfate / Q000633:toxicity*; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage* / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects; D007279:Injections, Subcutaneous; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D015398:Signal Transduction / Q000187:drug effects*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D051017:Apoptosis Regulatory Proteins; D053468:CARD Signaling Adaptor Proteins; D002352:Carrier Proteins; D004365:Drugs, Chinese Herbal; C498534:Pycard protein, mouse; D014409:Tumor Necrosis Factor-alpha; C061091:lithospermate B; D016264:Dextran Sulfate; D020170:Caspase 1
keywords: Apoptosis-associated speck-like protein containing; Caspase-1; Magnesium lithospermate B; NOD-like receptor protein; Tumor necrosis factor-α; Ulcerative colitis