title: Light-Up Probes Based on Fluorogens with Aggregation-Induced Emission Characteristics for Monoamine Oxidase-A Activity Study in Solution and in Living Cells.
abstract: Fluorogens with aggregation-induced emission (AIEgens) have emerged as a powerful and versatile platform for the development of novel biosensors. In this study, a series of water-soluble fluorescent probes based on tetraphenylethylene (TPE) were designed and synthesized for the detection of monoamine oxidases (MAOs) based on specific interactions between the probes and the proteins. Among the six probes developed, t-TPEM displays a significant fluorescence increase upon introduction of MAOs. Of particular significance is that the fluorescence of t-TPEM in the presence of MAO-A is 21-fold higher than other proteins including MAO-B. Lineweaver-Burk plots reveal that t-TPEM acts as an uncompetitive inhibitor of MAO-A with Ki = 17.1 μM, which confirms its good binding affinity toward MAO-A. Furthermore, a cell imaging experiment reveals that t-TPEM is able to selectively monitor the activity of MAO-A which is localized in mitochondria of MCF-7 cells.
authors.affiliation: Department of General Surgery, Jinhua Central Hospital , Jinhua 321000, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Shen
authors.affiliation: Institute of Bioengineering, Zhejiang University of Technology , Chaowang Road 18, Hangzhou 310014, China.
authors.forename: Jiajun
authors.initials: J
authors.lastname: Yu
authors.affiliation: Institute of Bioengineering, Zhejiang University of Technology , Chaowang Road 18, Hangzhou 310014, China.
authors.forename: Jingyan
authors.initials: J
authors.lastname: Ge
authors.affiliation: Department of Chemical and Biomolecular Engineering, National University of Singapore , 4 Science Drive 4 117585, Singapore.
authors.forename: Ruoyu
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Institute of Bioengineering, Zhejiang University of Technology , Chaowang Road 18, Hangzhou 310014, China.|Institute of Biotechnology, RWTH Aachen University , Worringerweg 3, 52074 Aachen, Germany.
authors.forename: Feng
authors.initials: F
authors.lastname: Cheng
authors.affiliation: Institute of Bioengineering, Zhejiang University of Technology , Chaowang Road 18, Hangzhou 310014, China.
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of General Surgery, Jinhua Central Hospital , Jinhua 321000, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Fan
authors.affiliation: Department of General Surgery, Jinhua Central Hospital , Jinhua 321000, China.
authors.forename: Shian
authors.initials: S
authors.lastname: Yu
authors.affiliation: Department of Chemical and Biomolecular Engineering, National University of Singapore , 4 Science Drive 4 117585, Singapore.
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
authors.affiliation: Institute of Bioengineering, Zhejiang University of Technology , Chaowang Road 18, Hangzhou 310014, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhu
mesh_terms: D001665:Binding Sites; D002470:Cell Survival; D005030:Ethylenes / Q000737:chemistry; D005456:Fluorescent Dyes / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D008024:Ligands; D061986:MCF-7 Cells; D008995:Monoamine Oxidase / Q000378:metabolism*; D012996:Solutions; D013050:Spectrometry, Fluorescence
chemical_list: D005030:Ethylenes; D005456:Fluorescent Dyes; D008024:Ligands; D012996:Solutions; C036216:ethylene; D008995:Monoamine Oxidase
keywords: AIEgen; aggregation-induced emission; enzymatic assay; monoamine oxidase-A; tetraphenylethylene