title: Preparation of Nonionic Vesicles Using the Supercritical Carbon Dioxide Reverse Phase Evaporation Method and Analysis of Their Solution Properties.
abstract: We have previously reported a new preparation method for liposomes using supercritical carbon dioxide (scCO2) as a solvent, referred to as the supercritical carbon dioxide reverse phase evaporation (scRPE) method. In our previous work, addition of ethanol to scCO2 as a co-solvent was needed, because lipid molecules had to be dissolved in scCO2 to form liposomes. In this new study, niosomes (nonionic surfactant vesicles) were prepared from various nonionic surfactants using the scRPE method. Among the nonionic surfactants tested were polyoxyethylene (6) stearylether (C18EO6), polyoxyethylene (5) phytosterolether (BPS-5), polyoxyethylene (6) sorbitan stearylester (TS-106V), and polyoxyethylene (4) sorbitan stearylester (Tween 61). All these surfactants have hydrophilic-lipophilic balance values (HLBs) around 9.5 to 9.9, and they can all form niosomes using the scRPE method even in the absence of ethanol. The high solubility of these surfactants in scCO2 was shown to be an important factor in yielding niosomes without ethanol addition. The niosomes prepared with the scRPE method had higher trapping efficiencies than those prepared using the conventional Bangham method, since the scRPE method gives a large number of unilamellar vesicles while the Bangham method gives multilamellar vesicles. Polyoxyethylene-type nonionic surfactants with HLB values from 9.5 to 9.9 were shown to be optimal for the preparation of niosomes with the scRPE method.
authors.affiliation: Cosmos Technical Center Co., Ltd.
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Yamaguchi
authors.forename: Koji
authors.initials: K
authors.lastname: Tsuchiya
authors.forename: Kenichi
authors.initials: K
authors.lastname: Sakai
authors.forename: Masahiko
authors.initials: M
authors.lastname: Abe
authors.forename: Hideki
authors.initials: H
authors.lastname: Sakai
mesh_terms: D002245:Carbon Dioxide / Q000737:chemistry*; D055598:Chemical Phenomena; D000431:Ethanol / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D008081:Liposomes; D011092:Polyethylene Glycols / Q000737:chemistry; D012995:Solubility; D012996:Solutions; D013501:Surface-Active Agents; D014835:Volatilization
chemical_list: D008081:Liposomes; D012996:Solutions; D013501:Surface-Active Agents; D002245:Carbon Dioxide; D000431:Ethanol; D011092:Polyethylene Glycols