title: [Validation of GlobalFiler® PCR Amplification Kit and the STR Polymorphism].
abstract: OBJECTIVE
  To test the technical parameters of GlobalFiler® PCR Amplification Kit for its application to forensic application value and to investigate the genetic polymorphisms.
  
  
  METHODS
  The validation was conducted in sensitivity, mixed samples, species specificity, adaptability, survivability, consistency, peak height balance and stability. The amplification and detection of the genomic DNA from 373 unrelated individuals from Beijing Han nationality were extracted by automation workstation.
  
  
  RESULTS
  Global-Filer® PCR Amplification Kit was adaptive to some mixed, degraded and inhibited samples. The power of sensitivity and adaptability and peak height balance showed well. The distributions of genotype frequencies for 21 STR loci in the population were all in accordance with Hardy-Weinberg equilibrium (P > 0.05). The PIC value of the 21 STR loci was among 0.536 to 0.940; the H value was among 0.558 to 0.933; the DP value was among 0.783 to 0.992; the PE value was among 0.243 to 0.874.
  
  
  CONCLUSIONS
  GlobalFiler® PCR Amplification Kit is suitable for criminal cases and DNA database in forensic practice. And 21 STR loci in Beijing Han nationality have high polymorphism, which have application value in forensic practice and population genetics.
authors.forename: Zhi-yong
authors.initials: ZY
authors.lastname: Lu
authors.forename: Lu-yan
authors.initials: LY
authors.lastname: Xue
authors.forename: Qing-xia
authors.initials: QX
authors.lastname: Zhang
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhao
authors.forename: Jin-jie
authors.initials: JJ
authors.lastname: Liu
authors.forename: Hui
authors.initials: H
authors.lastname: Tang
mesh_terms: D044466:Asian People / Q000208:ethnology* / Q000235:genetics*; D000068476:Beijing; D030561:Databases, Nucleic Acid; D005006:Ethnicity; D005787:Gene Frequency; D056426:Genetic Loci / Q000235:genetics; D005828:Genetics, Population; D005838:Genotype; D006801:Humans; D016133:Polymerase Chain Reaction / Q000295:instrumentation* / Q000592:standards*; D011110:Polymorphism, Genetic; D015203:Reproducibility of Results; D013045:Species Specificity