title: Automated data collection in single particle electron microscopy.
abstract: Automated data collection is an integral part of modern workflows in single particle electron microscopy (EM) research. This review surveys the software packages available for automated single particle EM data collection. The degree of automation at each stage of data collection is evaluated, and the capabilities of the software packages are described. Finally, future trends in automation are discussed.
authors.affiliation: The National Resource for Automated Molecular Microscopy, New York Structural Biology Center, New York, NY 10027, USA Simons Electron Microscopy Center, New York Structural Biology Center, 89 Convent Ave, New York, NY 10027, USA Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA.
authors.forename: Yong Zi
authors.initials: YZ
authors.lastname: Tan
authors.affiliation: The National Resource for Automated Molecular Microscopy, New York Structural Biology Center, New York, NY 10027, USA Simons Electron Microscopy Center, New York Structural Biology Center, 89 Convent Ave, New York, NY 10027, USA.
authors.forename: Anchi
authors.initials: A
authors.lastname: Cheng
authors.affiliation: The National Resource for Automated Molecular Microscopy, New York Structural Biology Center, New York, NY 10027, USA Simons Electron Microscopy Center, New York Structural Biology Center, 89 Convent Ave, New York, NY 10027, USA Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA.
authors.forename: Clinton S
authors.initials: CS
authors.lastname: Potter
authors.affiliation: The National Resource for Automated Molecular Microscopy, New York Structural Biology Center, New York, NY 10027, USA Simons Electron Microscopy Center, New York Structural Biology Center, 89 Convent Ave, New York, NY 10027, USA Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA bcarr@nysbc.org.
authors.forename: Bridget
authors.initials: B
authors.lastname: Carragher
mesh_terms: D001331:Automation; D020285:Cryoelectron Microscopy / Q000295:instrumentation / Q000379:methods*; D003625:Data Collection; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D012984:Software
keywords: automation; data collection; high throughput; single particle electron microscopy; software packages; target acquisition