title: Genome-wide identification of conserved microRNA and their response to drought stress in Dongxiang wild rice (Oryza rufipogon Griff.).
abstract: OBJECTIVE
  To identify drought stress-responsive conserved microRNA (miRNA) from Dongxiang wild rice (Oryza rufipogon Griff., DXWR) on a genome-wide scale, high-throughput sequencing technology was used to sequence libraries of DXWR samples, treated with and without drought stress.
  
  
  RESULTS
  505 conserved miRNAs corresponding to 215 families were identified. 17 were significantly down-regulated and 16 were up-regulated under drought stress. Stem-loop qRT-PCR revealed the same expression patterns as high-throughput sequencing, suggesting the accuracy of the sequencing result was high. Potential target genes of the drought-responsive miRNA were predicted to be involved in diverse biological processes. Furthermore, 16 miRNA families were first identified to be involved in drought stress response from plants.
  
  
  CONCLUSIONS
  These results present a comprehensive view of the conserved miRNA and their expression patterns under drought stress for DXWR, which will provide valuable information and sequence resources for future basis studies.
authors.affiliation: College of Life Sciences, Jiangxi Normal University, Nanchang, 330022, China.
authors.forename: Fantao
authors.initials: F
authors.lastname: Zhang
authors.affiliation: College of Life Sciences, Jiangxi Normal University, Nanchang, 330022, China.
authors.forename: Xiangdong
authors.initials: X
authors.lastname: Luo
authors.affiliation: College of Life Sciences, Jiangxi Normal University, Nanchang, 330022, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: College of Life Sciences, Jiangxi Normal University, Nanchang, 330022, China. xiejiankun11@163.com.
authors.forename: Jiankun
authors.initials: J
authors.lastname: Xie
mesh_terms: D001483:Base Sequence; D017124:Conserved Sequence; D055864:Droughts; D018506:Gene Expression Regulation, Plant; D059014:High-Throughput Nucleotide Sequencing; D035683:MicroRNAs / Q000235:genetics*; D012275:Oryza / Q000235:genetics* / Q000254:growth & development; D040641:Quantitative Trait Loci; D018749:RNA, Plant / Q000235:genetics; D013312:Stress, Physiological
chemical_list: D035683:MicroRNAs; D018749:RNA, Plant
keywords: Dongxiang wild rice; Drought resistance; Genetic resource; High-throughput sequencing; microRNA