title: [Role of tumour cell invasion/migration in the vascularisation of experimental lung metastases].
abstract: Treatment of patients with lung metastases remains a major challenge. A possible target for therapies is the inhibition of vascularization of metastases. Our study aimed to determine the possible mechanisms of the experimental lung metastasis vascularisation for tumours of various origins. We created lung metastases by intravenous injection of five tumour cell lines (HT1080, HT25, B16, C26 and MATB). Each cell line showed the same vascularisation type. Tumours gained vasculature by advancing through the alveolar spaces thereby incorporating the pre-existing alveolar capillaries (i.e. vessel co-option). From the alveolar spaces tumours entered into the alveolar walls. The tumour cells during the invasion/migration separated the pneumocytes from the capillaries. During this process the basement membrane was split into an epithelial and an endothelial layer. The heavily compressed pneumocytes inside the tumour became fragmented but the incorporated and stripped vessels remained functional, so they were able to provide blood supply for the metastases.
authors.affiliation: I. Sz. Patológiai és Kísérleti Rákkutató Intézet, Semmelweis Egyetem, Budapest, Hungary. nesszy20@gmail.com.
authors.forename: Vanessza
authors.initials: V
authors.lastname: Szabó
authors.affiliation: Országos Onkológiai Intézet, Budapest, Hungary.
authors.forename: Edina
authors.initials: E
authors.lastname: Bugyik
authors.affiliation: I. Sz. Patológiai és Kísérleti Rákkutató Intézet, Semmelweis Egyetem, Budapest, Hungary. nesszy20@gmail.com.
authors.forename: Katalin
authors.initials: K
authors.lastname: Dezsõ
authors.affiliation: Országos Korányi Tbc és Pulmonológiai Intézet, Budapest, Hungary.
authors.forename: József
authors.initials: J
authors.lastname: Tóvári
authors.affiliation: Mellkassebészeti Osztály, Bécsi Orvostudományi Egyetem, Bécs, Austria.
authors.forename: Balázs
authors.initials: B
authors.lastname: Döme
authors.affiliation: Molekuláris Onkológia Kutatócsoport, MTA-SE, Budapest, Hungary.
authors.forename: Sándor
authors.initials: S
authors.lastname: Paku