title: A recombinant nucleocapsid protein-based indirect enzyme-linked immunosorbent assay to detect antibodies against porcine deltacoronavirus.
abstract: Recently, porcine deltacoronavirus (PDCoV) has been proven to be associated with enteric disease in piglets. Diagnostic tools for serological surveys of PDCoV remain in the developmental stage when compared with those for other porcine coronaviruses. In our study, an indirect enzyme-linked immunosorbent assay (ELISA) (rPDCoV-N-ELISA) was developed to detect antibodies against PDCoV using a histidine-tagged recombinant nucleocapsid (N) protein as an antigen. The rPDCoV-N-ELISA did not cross-react with antisera against porcine epidemic diarrhea virus, swine transmissible gastroenteritis virus, porcine group A rotavirus, classical swine fever virus, porcine circovirus-2, porcine pseudorabies virus, and porcine reproductive and respiratory syndrome virus; the receiver operating characteristic (ROC) curve analysis revealed 100% sensitivity and 90.4% specificity of the rPDCoV-N-ELISA based on samples of known status (n=62). Analyses of field samples (n=319) using the rPDCoV-N-ELISA indicated that 11.59% of samples were positive for antibodies against PDCoV. These data demonstrated that the rPDCoV-N-ELISA can be used for epidemiological investigations of PDCoV and that PDCoV had a low serum prevalence in pig population in Heilongjiang province, northeast China.
authors.affiliation: College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, No. 5 Xinfeng Road, Sartu District, Daqing 163319, P.R. China.
authors.forename: Mingjun
authors.initials: M
authors.lastname: Su
authors.forename: Chunqiu
authors.initials: C
authors.lastname: Li
authors.forename: Donghua
authors.initials: D
authors.lastname: Guo
authors.forename: Shan
authors.initials: S
authors.lastname: Wei
authors.forename: Xinyu
authors.initials: X
authors.lastname: Wang
authors.forename: Yufei
authors.initials: Y
authors.lastname: Geng
authors.forename: Shuang
authors.initials: S
authors.lastname: Yao
authors.forename: Jing
authors.initials: J
authors.lastname: Gao
authors.forename: Enyu
authors.initials: E
authors.lastname: Wang
authors.forename: Xiwen
authors.initials: X
authors.lastname: Zhao
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Wang
authors.forename: Jianfa
authors.initials: J
authors.lastname: Wang
authors.forename: Rui
authors.initials: R
authors.lastname: Wu
authors.forename: Li
authors.initials: L
authors.lastname: Feng
authors.forename: Dongbo
authors.initials: D
authors.lastname: Sun
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis* / Q000276:immunology; D002681:China; D003332:Coronaviridae / Q000276:immunology*; D003333:Coronaviridae Infections / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D019590:Nucleocapsid Proteins / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis / Q000276:immunology / Q000821:virology*
chemical_list: D000914:Antibodies, Viral; D019590:Nucleocapsid Proteins; D011994:Recombinant Proteins