title: Genetic variants of vascular endothelial growth factor are associated with recurrent implantation failure in Korean women.
abstract: Vascular endothelial growth factor (VEGF) is involved in embryonic development, decidual vascularization and placenta angiogenesis. This study was performed to determine whether there is an association between genetic polymorphisms in the VEGF gene and the development of recurrent implantation failure (RIF) in Korean women. A total of 119 women diagnosed with RIF and 236 control subjects were genotyped for VEGF polymorphic sites including rs833061 (-460T>C), rs25648 (-7C>T) and rs3025020 (-583C>T) using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assays and real-time PCR. The VEGF rs833061 C allele and rs25648 T allele were significantly associated with increased RIF risk (odds ratio [OR] = 1.813 [1.161-2.831], P = 0.009, OR = 2.213 [1.254-3.903], P = 0.005). The rs833061/rs25648 TC/CT, TC/CT+TT, and rs833061/rs3025020 TC+CC/TT genotypes were more frequent in the RIF group compared with the control group (OR = 2.130 [1.092-4.156], P = 0.025, OR = 2.130 [1.092-4.156], OR = 4.261 [1.163-15.620], P = 0.028, respectively). The results of this study suggests that VEGF polymorphisms are associated with RIF development. Therefore, we postulate that VEGF polymorphisms might be useful markers to predict RIF development. Further studies are warranted to elucidate the role of VEGF variants and RIF development.
authors.affiliation: Department of Obstetrics and Gynecology, CHA Gangnam Medical Center, CHA University, Seoul, Republic of Korea.
authors.forename: Yong Wook
authors.initials: YW
authors.lastname: Jung
authors.affiliation: Department of Biomedical Science, College of Life Science, CHA University, Seongnam-si, Republic of Korea.
authors.forename: Jung Oh
authors.initials: JO
authors.lastname: Kim
authors.affiliation: Department of Biomedical Science, College of Life Science, CHA University, Seongnam-si, Republic of Korea.
authors.forename: HyungChul
authors.initials: H
authors.lastname: Rah
authors.affiliation: Department of Obstetrics and Gynecology, CHA Bundang Medical Center, CHA University, Seongnam-si, Republic of Korea.
authors.forename: Ji Hyang
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Department of Obstetrics and Gynecology, CHA Bundang Medical Center, CHA University, Seongnam-si, Republic of Korea.
authors.forename: Young Ran
authors.initials: YR
authors.lastname: Kim
authors.affiliation: Fertility Center of CHA Gangnam Medical Center, CHA University, Seoul, Republic of Korea.
authors.forename: Yubin
authors.initials: Y
authors.lastname: Lee
authors.affiliation: Fertility Center of CHA Gangnam Medical Center, CHA University, Seoul, Republic of Korea. Electronic address: wooslee@cha.ac.kr.
authors.forename: Woo Sik
authors.initials: WS
authors.lastname: Lee
authors.affiliation: Department of Biomedical Science, College of Life Science, CHA University, Seongnam-si, Republic of Korea. Electronic address: nkkim@cha.ac.kr.
authors.forename: Nam Keun
authors.initials: NK
authors.lastname: Kim
mesh_terms: D000328:Adult; D000483:Alleles; D016022:Case-Control Studies; D010064:Embryo Implantation; D005260:Female; D020022:Genetic Predisposition to Disease; D014644:Genetic Variation; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D007247:Infertility, Female / Q000628:therapy*; D007621:Karyotyping; D016017:Odds Ratio; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D011446:Prospective Studies; D056910:Republic of Korea; D012306:Risk; D017211:Treatment Failure; D016896:Treatment Outcome; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics*
chemical_list: C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A
keywords: VEGF; female infertility; genetic association study; recurrent implantation failure; single nucleotide polymorphism