title: Mechanisms of the beneficial effect of sevoflurane in liver ischemia/reperfusion injury.
abstract: OBJECTIVE
  To evaluate the underlying mechanisms by which sevoflurane protects the liver against ischemia/reperfusion injury evaluate the mechanism by which sevoflurane exerts this protective effect.
  
  
  METHODS
  Twenty-six rats were subjected to partial ischemia/reperfusion injury for 1h: one group received no treatment, one group received sevoflurane, and sham group of animals received laparotomy only. Four hours after reperfusion, levels of alanine and aspartate aminotransferases, tumor necrosis factor-a, and interleukins 6 and 10 were measured. Analyses of mitochondrial oxidation and phosphorylation, malondialdehyde content, histology, and pulmonary vascular permeability were performed.
  
  
  RESULTS
  Serum levels of alanine and aspartate aminotransferases were significantly lower in the sevoflurane group compared to untreated controls (p<0.05). The sevoflurane group also showed preservation of liver mitochondrial function compared to untreated controls (p<0.05). Sevoflurane administration did not alter increases in serum levels of tumor necrosis factor-a, and interleukins 6 and 10. Sevoflurane treatment significantly reduced the coagulative necrosis induced by ischemia/reperfusion (p<0.05). Pulmonary vascular permeability was preserved in the sevoflurane group compared to untreated controls.
  
  
  CONCLUSIONS
  Sevoflurane administration protects the liver against ischemia/reperfusion injury, via preservation of mitochondrial function, and also preserves lung vascular permeability.
authors.affiliation: School of Medicine, Universidade de São Paulo, Brazil.
authors.forename: Fernanda Paula
authors.initials: FP
authors.lastname: Cavalcante
authors.affiliation: School of Medicine, USP, Sao Paulo, SP, Brazil.
authors.forename: Ana Maria Mendonça
authors.initials: AM
authors.lastname: Coelho
authors.affiliation: School of Medicine, USP, Sao Paulo, SP, Brazil.
authors.forename: Marcel Cerqueira Cesar
authors.initials: MC
authors.lastname: Machado
authors.affiliation: School of Medicine, USP, Sao Paulo, SP, Brazil.
authors.forename: Sandra Nassa
authors.initials: SN
authors.lastname: Sampietre
authors.affiliation: School of Medicine, USP, Sao Paulo, SP, Brazil.
authors.forename: Rosely Antunes
authors.initials: RA
authors.lastname: Patzina
authors.affiliation: School of Medicine, USP, Sao Paulo, SP, Brazil.
authors.forename: Márcio Augusto
authors.initials: MA
authors.lastname: Diniz
authors.affiliation: School of Medicine, USP, Sao Paulo, SP, Brazil.
authors.forename: Eleazar
authors.initials: E
authors.lastname: Chaib
authors.affiliation: School of Medicine, USP, Sao Paulo, SP, Brazil.
authors.forename: Luiz Augusto Carneiro
authors.initials: LA
authors.lastname: D'Albuquerque
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D018685:Anesthetics, Inhalation / Q000494:pharmacology*; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D002199:Capillary Permeability / Q000187:drug effects; D016207:Cytokines / Q000097:blood; D007511:Ischemia / Q000473:pathology / Q000517:prevention & control*; D015227:Lipid Peroxidation; D008099:Liver / Q000098:blood supply* / Q000473:pathology; D008297:Male; D008738:Methyl Ethers / Q000494:pharmacology*; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000502:physiology; D009336:Necrosis; D010766:Phosphorylation; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000473:pathology / Q000517:prevention & control*; D015203:Reproducibility of Results; D000077149:Sevoflurane; D013997:Time Factors
chemical_list: D018685:Anesthetics, Inhalation; D016207:Cytokines; D008738:Methyl Ethers; D000077149:Sevoflurane; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase