title: Application of succulent plant leaves for Agrobacterium infiltration-mediated protein production.
abstract: When expressing plant cell wall degrading enzymes in the widely used tobacco (Nicotiana benthamiana) after Agrobacterium infiltration, difficulties arise due to the thin leaf structure. Thick leaved succulents, Kalanchoe blossfeldiana and Hylotelephium telephium, were tested as alternatives. A xyloglucanase, as well as a xyloglucanase inhibitor protein was successfully produced.
authors.affiliation: USDA-ARS, Genetic improvement of Fruits and Vegetables Laboratory, 10300 Baltimore Ave, Beltsville, MD 20705, United States. Electronic address: richard.jones@ars.usda.gov.
authors.forename: Richard W
authors.initials: RW
authors.lastname: Jones
mesh_terms: D060054:Agrobacterium / Q000201:enzymology / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics; D029627:Crassulaceae / Q000378:metabolism; D017343:Genes, Plant; D006026:Glycoside Hydrolases / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000235:genetics; D031962:Kalanchoe / Q000382:microbiology*; D018515:Plant Leaves / Q000382:microbiology*; D030821:Plants, Genetically Modified; D010957:Plasmids / Q000235:genetics; D031963:Sedum / Q000382:microbiology; D014026:Nicotiana / Q000382:microbiology
chemical_list: D001426:Bacterial Proteins; D006026:Glycoside Hydrolases; C072818:xyloglucan endo(1-4)-beta-D-glucanase
keywords: Agrobacterium; Crassulaceae; Xyloglucanase; Xyloglucanase inhibitor