title: DEVELOPMENT OF CHROMATOGRAPHIC METHOD FOR DETERMINATION OF DRUGS REDUCING CHOLESTEROL LEVEL--STATINS AND EZETIMIBE.
abstract: The presented developed HPLC method and GC method may be used to separate and determine all analyzed 3-hydroxy-3-methylglutaryl-coenzyme A reductase inhibitors (statins) and ezetimibe using a single columns and a uniform methodology. In order to perform qualitative and quantitative tests of statins and ezetimibe the Symmetry C18 column 250 mm x 4.6 mm, 5 µm, the mobile phase: acetonitrile:water (70:30, v/v), adjusted to pH = 2.5 and a spectrophotometric detector for the HPLC method were used. For GC method column HP-1; 30 m x 0.25 mm x 0.25 µm and FID detector were selected. All results and statistical data obtained indicate good method sensitivity and precision. The RSD values are appropriate for both newly developed methods.
authors.forename: Elżbieta
authors.initials: E
authors.lastname: Kublin
authors.forename: Ewa
authors.initials: E
authors.lastname: Malanowicz
authors.forename: Barbara
authors.initials: B
authors.lastname: Kaczmarska-Graczyk
authors.forename: Krystyna
authors.initials: K
authors.lastname: Czerwińska
authors.forename: Elżbieta
authors.initials: E
authors.lastname: Wyszomirska
authors.forename: Aleksander P
authors.initials: AP
authors.lastname: Mazurek
mesh_terms: D000924:Anticholesteremic Agents / Q000032:analysis*; D002849:Chromatography, Gas / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D000069438:Ezetimibe / Q000032:analysis*; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors / Q000032:analysis*
chemical_list: D000924:Anticholesteremic Agents; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D000069438:Ezetimibe