title: Molecular Characterization by Using Next-Generation Sequencing of Plasmids Containing blaNDM-7 in Enterobacteriaceae from Calgary, Canada.
abstract: Enterobacteriaceae with blaNDM-7 are relatively uncommon and had previously been described in Europe, India, the United States, and Japan. This study describes the characteristics of Enterobacteriaceae (Klebsiella pneumoniae [n = 2], Escherichia coli [n = 2], Serratia marcescens [n = 1], and Enterobacter hormaechei [n = 1] isolates) with blaNDM-7 obtained from 4 patients from Calgary, Canada, from 2013 to 2014. The 46,161-bp IncX3 plasmids with blaNDM-7 are highly similar to other blaNDM-harboring IncX3 plasmids and, interestingly, showed identical structures within the different isolates. This finding may indicate horizontal transmission within our health region, or it may indicate contact with individuals from areas of endemicity within the hospital setting. Patients infected or colonized with bacteria containing blaNDM-7 IncX3 plasmids generate infection control challenges. Epidemiological and molecular studies are required to better understand the dynamics of transmission, the risk factors, and the reservoirs for bacteria harboring blaNDM-7. To the best of our knowledge, this is the first report of S. marcescens and E. hormaechei with blaNDM-7.
authors.affiliation: Public Health Research Institute Tuberculosis Center, Rutgers University, New Brunswick, New Jersey, USA.
authors.forename: L
authors.initials: L
authors.lastname: Chen
authors.affiliation: Division of Microbiology, Calgary Laboratory Services, University of Calgary, Alberta, Canada.
authors.forename: G
authors.initials: G
authors.lastname: Peirano
authors.affiliation: Division of Microbiology, Calgary Laboratory Services, University of Calgary, Alberta, Canada Department of Pathology and Laboratory Medicine, University of Calgary, Alberta, Canada.
authors.forename: T
authors.initials: T
authors.lastname: Lynch
authors.affiliation: Public Health Research Institute Tuberculosis Center, Rutgers University, New Brunswick, New Jersey, USA.
authors.forename: K D
authors.initials: KD
authors.lastname: Chavda
authors.affiliation: Division of Microbiology, Calgary Laboratory Services, University of Calgary, Alberta, Canada Department of Pathology and Laboratory Medicine, University of Calgary, Alberta, Canada Department of Medicine, University of Calgary, Alberta, Canada Snyder Institute for Chronic Diseases, University of Calgary, Alberta, Canada.
authors.forename: D B
authors.initials: DB
authors.lastname: Gregson
authors.affiliation: Division of Microbiology, Calgary Laboratory Services, University of Calgary, Alberta, Canada Department of Pathology and Laboratory Medicine, University of Calgary, Alberta, Canada Department of Medicine, University of Calgary, Alberta, Canada Snyder Institute for Chronic Diseases, University of Calgary, Alberta, Canada.
authors.forename: D L
authors.initials: DL
authors.lastname: Church
authors.affiliation: Division of Microbiology, Calgary Laboratory Services, University of Calgary, Alberta, Canada Department of Pathology and Laboratory Medicine, University of Calgary, Alberta, Canada Department of Medicine, University of Calgary, Alberta, Canada Snyder Institute for Chronic Diseases, University of Calgary, Alberta, Canada.
authors.forename: J
authors.initials: J
authors.lastname: Conly
authors.affiliation: Public Health Research Institute Tuberculosis Center, Rutgers University, New Brunswick, New Jersey, USA.
authors.forename: B N
authors.initials: BN
authors.lastname: Kreiswirth
authors.affiliation: Division of Microbiology, Calgary Laboratory Services, University of Calgary, Alberta, Canada Department of Pathology and Laboratory Medicine, University of Calgary, Alberta, Canada Department of Microbiology, Immunology, and Infectious Diseases, University of Calgary, Alberta, Canada Snyder Institute for Chronic Diseases, University of Calgary, Alberta, Canada Department of Medical Microbiology, University of Pretoria, Pretoria, South Africa johann.pitout@cls.ab.ca.
authors.forename: J D
authors.initials: JD
authors.lastname: Pitout
mesh_terms: D000416:Alberta / Q000453:epidemiology; D001426:Bacterial Proteins / Q000235:genetics; D004755:Enterobacteriaceae / Q000187:drug effects* / Q000235:genetics*; D004756:Enterobacteriaceae Infections / Q000453:epidemiology / Q000382:microbiology; D005260:Female; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods; D006761:Hospitals; D006801:Humans; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000235:genetics; D008297:Male; D008826:Microbial Sensitivity Tests; D010957:Plasmids / Q000235:genetics*; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D001618:beta-Lactamases; C063912:carbapenemase