title: DETERMINATION OF FLUCONAZOLE IN HUMAN PLASMA BY REVERSE PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY.
abstract: A rapid, simple and reproducible high performance liquid chromatographic method was developed and validated for determination of fluconazole in human plasma. The separation was performed on MZ C8 column (125 x 4 mm, 5 µm) using acetonitrile - potassium dihydrogen phosphate buffer (15 : 85, v/v), pH 3.0, as the mobile phase at a flow rate of 1.5 mL/min. The wavelength was set at 261 nm. The assay enables the measurement of fluconazole for therapeutic drug monitoring with a minimum quantification limit of 20 ng/mL. The method involves simple, protein precipitation procedure and analytical recovery was complete. The calibration curve was linear over the concentration range 0.1-4 µg/mL. The coefficients of variation for inter-day and intra-day assay were found to be less than 10%.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Safaei
authors.forename: Eskandar
authors.initials: E
authors.lastname: Alipour
authors.forename: Alireza
authors.initials: A
authors.lastname: Shafaati
authors.forename: Afshin
authors.initials: A
authors.lastname: Zarghp
mesh_terms: D000935:Antifungal Agents / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D015725:Fluconazole / Q000097:blood*; D006801:Humans; D008297:Male
chemical_list: D000935:Antifungal Agents; D015725:Fluconazole