title: Separation of Aeruginosin-865 from Cultivated Soil Cyanobacterium (Nostoc sp.) by Centrifugal Partition Chromatography combined with Gel Permeation Chromatography.
abstract: Aeruginosin-865 was isolated from cultivated soil cyanobacteria using a combination of centrifugal partition chromatography (CPC) and gel permeation chromatography. The solubility of Aer-865 in different solvents was evaluated using the conductor-like screening model for real solvents (COSMO-RS). The CPC separation was performed in descending mode with a biphasic solvent system composed of water-n-BuOH-acetic acid (5:4:1, v/v/v). The upper phase was used as a stationary phase, whereas the lower phase was employed as a mobile phase at a flow rate of 10 mL/min. The revolution speed and temperature of the separation column were 1700 rpm and 25 degrees C, respectively. Preparative CPC separation followed by gel permeation chromatography was performed on 50 mg of crude extract yielding Aer-865 (3.5 mg), with a purity over 95% as determined by HPLC. The chemical identity of the isolated compound was confirmed by comparing its spectroscopic data (UV, HRESI-MS, HRESI-MS/MS) with those of an authentic standard and data available in the literature.
authors.forename: José
authors.initials: J
authors.lastname: Cheel
authors.forename: Mirjana
authors.initials: M
authors.lastname: Minceva
authors.forename: Petra
authors.initials: P
authors.lastname: Urajová
authors.forename: Rabya
authors.initials: R
authors.lastname: Aslam
authors.forename: Pavel
authors.initials: P
authors.lastname: Hrouzek
authors.forename: Jiří
authors.initials: J
authors.lastname: Kopecký
mesh_terms: D002845:Chromatography / Q000379:methods*; D006020:Glycopeptides / Q000737:chemistry*; D055666:Lipopeptides / Q000737:chemistry*; D015394:Molecular Structure; D046937:Nostoc / Q000737:chemistry*
chemical_list: D006020:Glycopeptides; D055666:Lipopeptides; C000589019:aeruginosin-865