title: Transduction of an immortalized olfactory ensheathing glia cell line with the green fluorescent protein (GFP) gene: Evaluation of its neuroregenerative capacity as a proof of concept.
abstract: Olfactory ensheathing glia (OEG) cells are known to foster axonal regeneration of central nervous system (CNS) neurons. Several lines of reversibly immortalized human OEG (ihOEG) have been previously established that enabled to develop models for their validation in vitro and in vivo. In this work, a constitutively GFP-expressing ihOEG cell line was obtained, and named Ts14-GFP. Ts14-GFP neuroregenerative ability was similar to that found for the parental line Ts14 and it can be assayed using in vivo transplantation experimental paradigms, after spinal cord or optic nerve damage. Additionally, we have engineered a low-regenerative ihOEG line, hTL2, using lentiviral transduction of the large T antigen from SV40 virus, denominated from now on Ts12. Ts12 can be used as a low regeneration control in these experiments.
authors.affiliation: Neuroregeneration Group, Biotechnology Department, Universidad Francisco de Vitoria, Ctra. Pozuelo-Majadahonda, Km. 1.800, Pozuelo de Alarcón, 28223 Madrid, Spain.
authors.forename: N
authors.initials: N
authors.lastname: Plaza
authors.affiliation: Neuroregeneration Group, Biotechnology Department, Universidad Francisco de Vitoria, Ctra. Pozuelo-Majadahonda, Km. 1.800, Pozuelo de Alarcón, 28223 Madrid, Spain.
authors.forename: D
authors.initials: D
authors.lastname: Simón
authors.affiliation: Neuroregeneration Group, Biotechnology Department, Universidad Francisco de Vitoria, Ctra. Pozuelo-Majadahonda, Km. 1.800, Pozuelo de Alarcón, 28223 Madrid, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Sierra
authors.affiliation: Neuroregeneration Group, Biotechnology Department, Universidad Francisco de Vitoria, Ctra. Pozuelo-Majadahonda, Km. 1.800, Pozuelo de Alarcón, 28223 Madrid, Spain. Electronic address: mteresa.moreno@uam.es.
authors.forename: M T
authors.initials: MT
authors.lastname: Moreno-Flores
mesh_terms: D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics; D000957:Antigens, Viral, Tumor / Q000235:genetics; D001369:Axons / Q000502:physiology; D002460:Cell Line; D018920:Coculture Techniques; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D016086:Lentivirus / Q000235:genetics; D009416:Nerve Regeneration; D009457:Neuroglia / Q000166:cytology / Q000502:physiology*; D009830:Olfactory Bulb / Q000166:cytology*; D051381:Rats; D012165:Retinal Ganglion Cells / Q000166:cytology; D014161:Transduction, Genetic
chemical_list: D000952:Antigens, Polyomavirus Transforming; D000957:Antigens, Viral, Tumor; D049452:Green Fluorescent Proteins
keywords: Axonal regeneration; Green fluorescent protein (GFP); Immortalized human olfactory ensheathing glia (ihOEG); Proof of concept; Retinal ganglion neuron (RGN)