title: Fatty acid analysis of triacylglycerols: Preparation of fatty acid methyl esters for gas chromatography.
abstract: A method to prepare fatty acid methyl esters was developed for fatty acid analysis of triacylglycerols by gas chromatography (GC). Triacylglycerols were mixed with methanolic CH3ONa in hexane containing a mid-polar solvent for 10 s at room temperature. Under these conditions, trioleoylglycerol was converted to methyl oleate with an average yield of 99.3%. This procedure gave reliable and reproducible data on fatty acid compositions determined by GC.
authors.affiliation: Kyoto Integrated Science & Technology Bio-Analysis Center, Advanced Science, Technology & Management Research Institute of Kyoto (ASTEM), Kyoto 600-8813, Japan. Electronic address: ichihara@kpu.ac.jp.
authors.forename: Ken'ichi
authors.initials: K
authors.lastname: Ichihara
authors.affiliation: Kyoto Municipal Institute of Industrial Technology and Culture, Kyoto 600-8815, Japan.
authors.forename: Chihiro
authors.initials: C
authors.lastname: Kohsaka
authors.affiliation: Kyoto Municipal Institute of Industrial Technology and Culture, Kyoto 600-8815, Japan.
authors.forename: Naohiro
authors.initials: N
authors.lastname: Tomari
authors.affiliation: Kyoto Municipal Institute of Industrial Technology and Culture, Kyoto 600-8815, Japan.
authors.forename: Tamami
authors.initials: T
authors.lastname: Kiyono
authors.affiliation: Kyoto Municipal Institute of Industrial Technology and Culture, Kyoto 600-8815, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Wada
authors.affiliation: Kyoto Municipal Institute of Industrial Technology and Culture, Kyoto 600-8815, Japan.
authors.forename: Kiyoo
authors.initials: K
authors.lastname: Hirooka
authors.affiliation: Kyoto Municipal Institute of Industrial Technology and Culture, Kyoto 600-8815, Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Yamamoto
mesh_terms: D002849:Chromatography, Gas; D004952:Esters / Q000737:chemistry; D005227:Fatty Acids / Q000032:analysis*; D006586:Hexanes / Q000737:chemistry; D000432:Methanol / Q000737:chemistry; D009829:Oleic Acids / Q000032:analysis / Q000378:metabolism; D013696:Temperature; D014280:Triglycerides / Q000737:chemistry*
chemical_list: D004952:Esters; D005227:Fatty Acids; D006586:Hexanes; D009829:Oleic Acids; D014280:Triglycerides; C005576:methyl oleate; D000432:Methanol
keywords: Fatty acid analysis; Fatty acid methyl ester; Gas chromatography; Triacylglycerol