title: Complement Effectors of Inflammation in Cystic Fibrosis Lung Fluid Correlate with Clinical Measures of Disease.
abstract: In cystic fibrosis (CF), lung damage is mediated by a cycle of obstruction, infection, and inflammation. Here we explored complement inflammatory effectors in CF lung fluid. In this study soluble fractions (sols) from sputum samples of 15 CF patients were assayed for complement effectors and analyzed with clinical measurements. The pro-inflammatory peptide C5a was increased 4.8-fold (P = 0.04) in CF sols compared with controls. Incubation of CF sols with P. aeruginosa or S. aureus increased C5a concentration 2.3-fold (P = 0.02). A peptide inhibitor of complement C1 (PIC1) completely blocked the increase in C5a concentration from P. aeruginosa in CF sol in vitro (P = 0.001). C5a concentration in CF sol correlated inversely with body mass index (BMI) percentile in children (r = -0.77, P = 0.04). C3a, which has anti-inflammatory effects, correlated positively with FEV1% predicted (rs = 0.63, P = 0.02). These results suggest that complement effectors may significantly impact inflammation in CF lung fluid.
authors.affiliation: Department of Pediatrics, Eastern Virginia Medical School, 700 West Olney Road, Norfolk, Virginia, United States of America.|Children's Specialty Group, 811 Redgate Avenue, Norfolk, Virginia, United States of America.|Children's Hospital of The King's Daughters, 601 Children's Lane, Norfolk, Virginia, United States of America.
authors.forename: Laura A
authors.initials: LA
authors.lastname: Sass
authors.affiliation: Department of Pediatrics, Eastern Virginia Medical School, 700 West Olney Road, Norfolk, Virginia, United States of America.
authors.forename: Pamela S
authors.initials: PS
authors.lastname: Hair
authors.affiliation: Department of Pediatrics, Eastern Virginia Medical School, 700 West Olney Road, Norfolk, Virginia, United States of America.|Children's Hospital of The King's Daughters, 601 Children's Lane, Norfolk, Virginia, United States of America.
authors.forename: Amy M
authors.initials: AM
authors.lastname: Perkins
authors.affiliation: Department of Pediatrics, Eastern Virginia Medical School, 700 West Olney Road, Norfolk, Virginia, United States of America.|Children's Specialty Group, 811 Redgate Avenue, Norfolk, Virginia, United States of America.|Children's Hospital of The King's Daughters, 601 Children's Lane, Norfolk, Virginia, United States of America.|Department of Microbiology and Molecular Cell Biology, Eastern Virginia Medical School, 700 West Olney Road, Norfolk, Virginia, United States of America.
authors.forename: Tushar A
authors.initials: TA
authors.lastname: Shah
authors.affiliation: Department of Pediatrics, Eastern Virginia Medical School, 700 West Olney Road, Norfolk, Virginia, United States of America.|Children's Specialty Group, 811 Redgate Avenue, Norfolk, Virginia, United States of America.
authors.forename: Neel K
authors.initials: NK
authors.lastname: Krishna
authors.affiliation: Department of Pediatrics, Eastern Virginia Medical School, 700 West Olney Road, Norfolk, Virginia, United States of America.|Children's Specialty Group, 811 Redgate Avenue, Norfolk, Virginia, United States of America.|Children's Hospital of The King's Daughters, 601 Children's Lane, Norfolk, Virginia, United States of America.|Department of Microbiology and Molecular Cell Biology, Eastern Virginia Medical School, 700 West Olney Road, Norfolk, Virginia, United States of America.
authors.forename: Kenji M
authors.initials: KM
authors.lastname: Cunnion
mesh_terms: D001826:Body Fluids / Q000276:immunology*; D016022:Case-Control Studies; D002648:Child; D015936:Complement C5a / Q000378:metabolism*; D003550:Cystic Fibrosis / Q000276:immunology* / Q000503:physiopathology; D005500:Follow-Up Studies; D006801:Humans; D007249:Inflammation / Q000276:immunology* / Q000503:physiopathology; D008168:Lung / Q000276:immunology* / Q000503:physiopathology; D011379:Prognosis; D011552:Pseudomonas Infections / Q000276:immunology* / Q000382:microbiology / Q000503:physiopathology; D013183:Sputum / Q000276:immunology*; D013211:Staphylococcus aureus / Q000276:immunology
chemical_list: D015936:Complement C5a