title: New Treatment of Medullary and Papillary Human Thyroid Cancer: Biological Effects of Hyaluronic Acid Hydrogel Loaded With Quercetin Alone or in Combination to an Inhibitor of Aurora Kinase.
abstract: The aim of this paper is based on the use of a hyaluronic acid hydrogel of Quercetin tested alone and in combination to an inhibitor of Aurora Kinase type A and B (SNS-314) on human medullary and papillary thyroid cancer cells. Biological investigations were focused on the cellular uptake of the hydrogel, cell viability, antioxidant, and cytokines secretion studies. Quercetin delivered from hydrogel show a time and CD44 dependent interaction with both cell lines with significant anti-inflammatory effects. Combination of Quercetin and SNS-314 leads to a synergistic cytotoxic effect on medullary TT and papillary BCPAP cell lines with a significant reduction of the IC50 value. These results, highlights the importance of synergistic effect of the hyaluronic acid hydrogel of Quercetin with SNS-314 in the regulation of human thyroid cancer cell proliferation and emphasize the anti-tumor activity of these molecules. J. Cell. Physiol. 231: 1784-1795, 2016. © 2015 Wiley Periodicals, Inc.
authors.affiliation: Department of Anaesthesological, Surgical and Emergency Sciences, Second University of Naples, Naples, Italy.
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Quagliariello
authors.affiliation: Department of Anaesthesological, Surgical and Emergency Sciences, Second University of Naples, Naples, Italy.
authors.forename: Emilia
authors.initials: E
authors.lastname: Armenia
authors.affiliation: Department of Anaesthesological, Surgical and Emergency Sciences, Second University of Naples, Naples, Italy.
authors.forename: Caterina
authors.initials: C
authors.lastname: Aurilio
authors.affiliation: Department of Anaesthesological, Surgical and Emergency Sciences, Second University of Naples, Naples, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Rosso
authors.affiliation: Department of Anaesthesological, Surgical and Emergency Sciences, Second University of Naples, Naples, Italy.
authors.forename: Ottavia
authors.initials: O
authors.lastname: Clemente
authors.affiliation: Department of Anaesthesological, Surgical and Emergency Sciences, Second University of Naples, Naples, Italy.
authors.forename: Gabriele
authors.initials: G
authors.lastname: de Sena
authors.affiliation: Department of Medical, Surgical, Neurological, Metabolic, and Aging Sciences, Second University of Naples, Naples, Italy.
authors.forename: Manlio
authors.initials: M
authors.lastname: Barbarisi
authors.affiliation: Department of Anaesthesological, Surgical and Emergency Sciences, Second University of Naples, Naples, Italy.
authors.forename: Alfonso
authors.initials: A
authors.lastname: Barbarisi
mesh_terms: D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D000975:Antioxidants / Q000378:metabolism; D064096:Aurora Kinase A / Q000235:genetics / Q000378:metabolism*; D064107:Aurora Kinase B / Q000037:antagonists & inhibitors* / Q000378:metabolism; D002277:Carcinoma / Q000188:drug therapy* / Q000201:enzymology / Q000473:pathology; D018278:Carcinoma, Neuroendocrine / Q000188:drug therapy* / Q000201:enzymology / Q000473:pathology; D002291:Carcinoma, Papillary; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002626:Chemistry, Pharmaceutical; D016207:Cytokines / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004337:Drug Carriers; D004357:Drug Synergism; D006801:Humans; D018960:Hyaluronan Receptors / Q000378:metabolism; D006820:Hyaluronic Acid / Q000737:chemistry*; D020100:Hydrogels; D020128:Inhibitory Concentration 50; D010671:Phenylurea Compounds / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D047428:Protein Kinase Inhibitors / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D011794:Quercetin / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D013844:Thiazoles / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D000077273:Thyroid Cancer, Papillary; D013964:Thyroid Neoplasms / Q000188:drug therapy* / Q000201:enzymology / Q000473:pathology; D013997:Time Factors
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; C497483:CD44 protein, human; D016207:Cytokines; D004337:Drug Carriers; D018960:Hyaluronan Receptors; D020100:Hydrogels; D010671:Phenylurea Compounds; D047428:Protein Kinase Inhibitors; C532454:SNS 314; D013844:Thiazoles; D006820:Hyaluronic Acid; D011794:Quercetin; C576917:AURKA protein, human; C576921:AURKB protein, human; D064096:Aurora Kinase A; D064107:Aurora Kinase B