title: Determination of Ochratoxin A in Pig Kidneys by Immunoaffinity Cleanup and Ultra-High Performance Liquid Chromatography.
abstract: An accurate method was developed for determining ochratoxin A (OTA) in pig kidney using an immunoaffinity column for cleanup and ultra-HPLC/MS/MS for identification and quantification. Mean recoveries of OTA from kidney samples fortified at 0.10-5 μg/kg levels ranged from 74 to 92%, with RSDs of 4.6-7.5%. The LOD was estimated to be 0.03 μg/kg and the LOQ was 0.10 μg/kg based on an S/N in kidney of 3:1 and 10:1, respectively. The developed method was used for the determination of OTA in pig kidney. A survey of the OTA content of pig kidneys slaughtered in Chongqing, China, revealed that 35 out of 40 analyzed samples were contaminated; the OTA concentration in kidney ranged from trace level (0.03-0.1) to 0.323 μg/kg. This method was shown to be useful for accurately evaluating the intake of OTA from pig kidneys.
authors.affiliation: Bioengineering College of Chongqing University, Chongqing, 400030, People's Republic of China.
authors.forename: YaLi
authors.initials: Y
authors.lastname: Hou
authors.forename: Jinhui
authors.initials: J
authors.lastname: Zhou
authors.forename: YuPing
authors.initials: Y
authors.lastname: Li
authors.forename: JiangHua
authors.initials: J
authors.lastname: Xie
authors.forename: Li
authors.initials: L
authors.lastname: Zhou
authors.forename: FengLin
authors.initials: F
authors.lastname: Lv
mesh_terms: D000818:Animals; D002846:Chromatography, Affinity / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007668:Kidney / Q000737:chemistry*; D057230:Limit of Detection; D009793:Ochratoxins / Q000032:analysis*; D013552:Swine; D053719:Tandem Mass Spectrometry
chemical_list: D009793:Ochratoxins; C025589:ochratoxin A