title: Rapid extraction and determination of 25 bioactive constituents in Alpinia oxyphylla using microwave extraction with ultra high performance liquid chromatography with tandem mass spectrometry.
abstract: A novel and rapid microwave extraction and ultra high performance liquid chromatography with tandem mass spectrometry method was developed and validated for the simultaneous determination of 25 bioactive constituents (including two new constituents) in Fructus Alpinia oxyphylla. The optimized conditions of the microwave extraction was a microwave power of 300 W, extraction temperature of 80°C, solvent-to-solid ratio of 30 mL/g and extraction time of 8 min. Separation was achieved on a Waters ACQUITY UPLC(®) HSS C18 column (2.1 mm× 50 mm, 1.8 μm) using gradient elution with a mobile phase consisting of acetonitrile and 1 mM ammonium acetate at a flow rate of 0.2 mL/min. This is the first report of the simultaneous determination of 25 bioactive constituents in Fructus Alpinia oxyphylla by ultra high performance liquid chromatography with tandem mass spectrometry. The method was validated with good linearity, acceptable precision and accuracy. The validated method was successfully applied to determine the contents of 25 bioactive constituents in Fructus Alpinia oxyphylla from different sources and the analysis results were classified by hierarchical cluster analysis, which indicated the effect of different cultivation regions on the contents of constituents. This study provides powerful and practical guidance in the quality control of Alpinia oxyphylla and lays the foundation for further research of Alpinia oxyphylla.
authors.affiliation: Department of Pharmacy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan Province, P. R. China.|Key subject Laboratory of clinical medicine of colleges and universities in Henan province, Zhengzhou, Henan Province, P. R. China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Sun
authors.affiliation: Department of Pharmacy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan Province, P. R. China.|Key subject Laboratory of clinical medicine of colleges and universities in Henan province, Zhengzhou, Henan Province, P. R. China.
authors.forename: Xiangzhen
authors.initials: X
authors.lastname: Kong
authors.affiliation: Department of Pharmacy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan Province, P. R. China.|Key subject Laboratory of clinical medicine of colleges and universities in Henan province, Zhengzhou, Henan Province, P. R. China.
authors.forename: Lihua
authors.initials: L
authors.lastname: Zuo
authors.affiliation: Department of Pharmacy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan Province, P. R. China.|Key subject Laboratory of clinical medicine of colleges and universities in Henan province, Zhengzhou, Henan Province, P. R. China.
authors.forename: Jian
authors.initials: J
authors.lastname: Kang
authors.affiliation: Department of Pharmacy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan Province, P. R. China.|Key subject Laboratory of clinical medicine of colleges and universities in Henan province, Zhengzhou, Henan Province, P. R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Hou
authors.affiliation: Department of Pharmacy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan Province, P. R. China.|Key subject Laboratory of clinical medicine of colleges and universities in Henan province, Zhengzhou, Henan Province, P. R. China.
authors.forename: Xiaojian
authors.initials: X
authors.lastname: Zhang
mesh_terms: D032429:Alpinia / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D057230:Limit of Detection; D008872:Microwaves; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*
keywords: Bioactive constituents; Fructus Alpinia oxyphylla; Microwave extraction; Quantitative analysis