title: Cytrap Marker Systems for In Vivo Visualization of Cell Cycle Progression in Arabidopsis.
abstract: This chapter focuses on visualization of cell cycle progression in plant tissues using the dual-color marker system "Cytrap." The Cytrap line carries a part of Arabidopsis CDT1a fused to the red fluorescent protein (RFP) gene, which monitors the cell cycle phases from S to late G2 or early mitosis, and the G2/M-specific cyclin B1 marker fused to the green fluorescent protein (GFP) gene. We introduce growth conditions of Arabidopsis roots, the setup for microscopic observation, and analysis of obtained images.
authors.affiliation: Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara, 630-0192, Japan.|JST, CREST, Ikoma, Nara, 630-0192, Japan.
authors.forename: Shiori Sugamata
authors.initials: SS
authors.lastname: Aki
authors.affiliation: Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara, 630-0192, Japan. mumeda@bs.naist.jp.|JST, CREST, Ikoma, Nara, 630-0192, Japan. mumeda@bs.naist.jp.
authors.forename: Masaaki
authors.initials: M
authors.lastname: Umeda
mesh_terms: D017360:Arabidopsis / Q000166:cytology* / Q000235:genetics; D029681:Arabidopsis Proteins / Q000032:analysis* / Q000235:genetics; D002453:Cell Cycle; D018797:Cell Cycle Proteins / Q000032:analysis* / Q000235:genetics; D004867:Equipment Design; D008164:Luminescent Proteins / Q000032:analysis* / Q000235:genetics; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods; D061848:Optical Imaging / Q000295:instrumentation / Q000379:methods*; D018517:Plant Roots / Q000166:cytology* / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000032:analysis / Q000235:genetics; D000097573:Red Fluorescent Protein
chemical_list: D029681:Arabidopsis Proteins; D018797:Cell Cycle Proteins; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D000097573:Red Fluorescent Protein; C493651:CDT1a protein, Arabidopsis
keywords: Arabidopsis; Cell cycle; Fluorescent protein marker; Real-time imaging; Root