title: Target Gene Discovery for Novel Therapeutic Agents in Cancer Treatment.
abstract: Target identification of novel therapeutic drugs is pivotal for the establishment of (1) new anticancer regiments, (2) to control side effects of the drugs, and (3) to identify appropriate combinations with established drugs. Here, we describe several in vitro assays applicable to characterize different characteristics of tumor cells. Furthermore, we present a protocol for establishing a reporter gene system for in vivo imaging, allowing for the study of drug effects in small animal models.
authors.affiliation: Department of Radiology and Neuroradiology, Section Molecular Imaging, Christian-Albrechts-Universität zu Kiel, Am Botanischen Garten 14, 24118, Kiel, Germany. stiwari@email.uni-kiel.de.
authors.forename: Sanjay
authors.initials: S
authors.lastname: Tiwari
authors.affiliation: Institute for Human Genetics, University of Kiel and University Hospital Schleswig-Holstein, Campus Kiel, Arnold-Heller-Strasse 3, House 10, 24105, Kiel, Germany.
authors.forename: Ole
authors.initials: O
authors.lastname: Ammerpohl
authors.affiliation: Institute for Experimental Cancer Research, Section for Molecular Oncology, University Hospital Schleswig-Holstein, Campus Kiel, Arnold-Heller-Strasse 3, House 17, 24105, Kiel, Germany.
authors.forename: Holger
authors.initials: H
authors.lastname: Kalthoff
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D018929:Cell Culture Techniques / Q000379:methods; D002453:Cell Cycle / Q000187:drug effects; D055808:Drug Discovery / Q000379:methods*; D005434:Flow Cytometry / Q000379:methods; D017930:Genes, Reporter / Q000187:drug effects*; D006801:Humans; D016328:NF-kappa B / Q000032:analysis; D009369:Neoplasms / Q000188:drug therapy* / Q000235:genetics* / Q000473:pathology; D061848:Optical Imaging / Q000379:methods; D014162:Transfection / Q000379:methods; D051598:Whole Body Imaging / Q000379:methods
chemical_list: D000970:Antineoplastic Agents; D016328:NF-kappa B
keywords: Apoptosis; Cell cycle; FACS; Fluorescence; In vivo imaging; Tumor invasion