title: Pontibacter ummariensissp. nov., isolated from a hexachlorocyclohexane-contaminated soil.
abstract: A Gram-stain-negative, pinkish-red, rod-shaped bacterium designated strain NKM1Twas isolated from soil samples contaminated with hexachlorocyclohexane isomers, collected from Ummari village, Lucknow, Uttar Pradesh, India. The strain was characterized by a polyphasic taxonomic approach. 16S rRNA gene sequence analysis showed that strain NKM1Tclustered exclusively with members of the genusPontibacterof the familyCytophagaceae, phylumBacteroidetes.The 16S rRNA gene sequence similarity to type strains of the genusPontibacterranged from 96.7 to 93.8 %, with the highest sequence similarity found withPontibacter odishensisJC130T(96.7 %). Cells of strain NKM1Twere aerobic, non-flagellated and non-motile. Strain NKM1Twas catalase- and oxidase-positive but negative for nitrate reduction and hydrolysis of gelatin. The major fatty acids in strain NKM1Twere anteiso-C15 : 0, iso-C15 : 0, C16 : 0, C18 : 1ω9cand summed feature 3 (C16 : 1ω7cand/or C16 : 1ω6c). The polar lipid profile of strain NKM1Tshowed the presence of phosphatidylethanolamine and unknown glycolipids as well as aminolipids.sym-Homospermidine was found to be the major polyamine and menaquinone 7 (MK-7) was the major respiratory quinone. The DNA G+C content of strain NKM1Twas determined to be 58.6 mol%. Based on phenotypic, chemotaxonomic, physiological and biochemical evidence, it is proposed that isolate NKM1Trepresents a novel species that belongs to the genusPontibacter, for which the namePontibacter ummariensissp. nov. is proposed. The type strain is NKM1T( = DSM 100161T= KCTC 42944T= MCC 2777T).
authors.affiliation: Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi - 110007, India.
authors.forename: N K
authors.initials: NK
authors.lastname: Mahato
authors.affiliation: Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi - 110007, India.
authors.forename: C
authors.initials: C
authors.lastname: Tripathi
authors.affiliation: Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi - 110007, India.
authors.forename: N
authors.initials: N
authors.lastname: Nayyar
authors.affiliation: Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi - 110007, India.
authors.forename: A K
authors.initials: AK
authors.lastname: Singh
authors.affiliation: Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi - 110007, India.
authors.forename: R
authors.initials: R
authors.lastname: Lal