title: Aberrant expression of Arpin in human breast cancer and its clinical significance.
abstract: Arpin (Arp2/3 complex inhibitor), a novel protein found in 2013, plays a pivotal role in cell motility and migration. However, the precise role of Arpin in cancer is unclear. This study investigated the expression of Arpin in breast cancer and evaluated its correlation with the characteristics of clinical pathology and prognosis of breast cancer patients. Immunohistochemistry (IHC) for Arpin protein was performed on formalin-fixed, paraffin-embedded 176 breast cancer tissues and 43 normal breast tissues while qRT-PCR for Arpin mRNA with 104 paired tumour and paratumoural tissues from breast cancer patients respectively. The association of Arpin expression with clinical pathological features and survival was assessed in a retrospective cohort analysis of patients. The results showed that the expression of Arpin protein in cancer tissues was lower compared to that in normal breast and the expression of Arpin mRNA was also lower in cancer tissues than that in the matched paratumoural tissues. Among the 176 breast cancer patients, the lower expression of Arpin was significantly associated with advanced tumour, nodes and metastasis system stage, and the reduced Arpin expression was strongly associated with axillary lymph node metastasis using univariate and multivariate logistic regression analysis [odds ratio: 3.242; 95% confidence interval (CI): 1.526, 6.888; P < 0.05]. Furthermore, Arpin expression was an independent risk factor for recurrence-free survival (HR: 0.373; 95% CI: 0.171, 0.813; P < 0.05). As Arpin expression was first examined in human breast cancer tissues with qRT-PCR and IHC, our results suggest that Arpin downregulation may contribute to the initiation and development of breast cancer metastasis. Therefore, as a potential predictive marker, Arpin deserves future studies.
authors.affiliation: Medical Research Center, The Affiliated Hospital of Qingdao University, Qingdao, China.
authors.forename: Xiangping
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Ultrasonography, Qilu Hospital, Shandong University, Qingdao, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhao
authors.affiliation: Center of Diagnosis and Treatment of Breast Disease, The Affiliated Hospital of Qingdao University, Qingdao, China.
authors.forename: Haibo
authors.initials: H
authors.lastname: Wang
authors.affiliation: Center of Diagnosis and Treatment of Breast Disease, The Affiliated Hospital of Qingdao University, Qingdao, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Center of Diagnosis and Treatment of Breast Disease, The Affiliated Hospital of Qingdao University, Qingdao, China.
authors.forename: Mengdi
authors.initials: M
authors.lastname: Niu
authors.affiliation: Center of Diagnosis and Treatment of Breast Disease, The Affiliated Hospital of Qingdao University, Qingdao, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Sun
authors.affiliation: Center of Diagnosis and Treatment of Breast Disease, The Affiliated Hospital of Qingdao University, Qingdao, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Medical Research Center, The Affiliated Hospital of Qingdao University, Qingdao, China.
authors.forename: Ruyong
authors.initials: R
authors.lastname: Yao
authors.affiliation: Medical Research Center, The Affiliated Hospital of Qingdao University, Qingdao, China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Qu
mesh_terms: D001365:Axilla; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D001943:Breast Neoplasms / Q000378:metabolism* / Q000401:mortality; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D018572:Disease-Free Survival; D005260:Female; D015870:Gene Expression; D006801:Humans; D053208:Kaplan-Meier Estimate; D008198:Lymph Nodes / Q000378:metabolism* / Q000473:pathology; D008207:Lymphatic Metastasis; D042361:Mammary Glands, Human / Q000378:metabolism; D008875:Middle Aged; D015999:Multivariate Analysis; D012189:Retrospective Studies; D012307:Risk Factors
chemical_list: D014408:Biomarkers, Tumor; D002352:Carrier Proteins; C000604273:arpin protein, human
keywords: arpin; breast cancer; prognosis