title: The effects of quercetin-loaded PLGA-TPGS nanoparticles on ultraviolet B-induced skin damages in vivo.
abstract: Ultraviolet (UV) radiation has deleterious effects on living organisms, and functions as a tumor initiator and promoter. Multiple natural compounds, like quercetin, have been shown the protective effects on UV-induced damage. However, quercetin is extremely hydrophobic and limited by its poor percutaneous permeation and skin deposition. Here, we show that quercetin-loaded PLGA-TPGS nanoparticles could overcome low hydrophilicity of quercetin and improve its anti-UVB effect. Quercetin-loaded NPs can significantly block UVB irradiation induced COX-2 up-expression and NF-kB activation in Hacat cell line. Moreover, PLGA-TPGS NPs could efficiently get through epidermis and reach dermis. Treatment of mice with quercetin-loaded NPs also attenuates UVB irradiation-associated macroscopic and histopathological changes in mice skin. These results demonstrated that copolymer PLGA-TPGS could be used as drug nanocarriers against skin damage and disease. The findings provide an external use of PLGA-TPGS nanocarriers for application in the treatment of skin diseases.
  
  
  BACKGROUND
  Skin is the largest organ in the body and is subjected to ultraviolet (UV) radiation damage daily from the sun. Excessive exposure has been linked to the development of skin cancer. Hence, topically applied agents can play a major role in skin protection. In this article, the authors developed quercetin-loaded PLGA-TPGS nanoparticles and showed their anti-UVB effect.
authors.affiliation: The Shenzhen Key Lab of Gene and Antibody Therapy, and Division of Life and Health Sciences, Graduate School at Shenzhen, Tsinghua University, Shenzhen, P.R. China; School of Life Sciences, Tsinghua University, Beijing, P.R. China.
authors.forename: Xianbing
authors.initials: X
authors.lastname: Zhu
authors.affiliation: The Shenzhen Key Lab of Gene and Antibody Therapy, and Division of Life and Health Sciences, Graduate School at Shenzhen, Tsinghua University, Shenzhen, P.R. China; School of Life Sciences, Tsinghua University, Beijing, P.R. China.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Zeng
authors.affiliation: The Shenzhen Key Lab of Gene and Antibody Therapy, and Division of Life and Health Sciences, Graduate School at Shenzhen, Tsinghua University, Shenzhen, P.R. China; School of Life Sciences, Tsinghua University, Beijing, P.R. China.
authors.forename: Xudong
authors.initials: X
authors.lastname: Zhang
authors.affiliation: The Shenzhen Key Lab of Gene and Antibody Therapy, and Division of Life and Health Sciences, Graduate School at Shenzhen, Tsinghua University, Shenzhen, P.R. China; School of Life Sciences, Tsinghua University, Beijing, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Cao
authors.affiliation: The Shenzhen Key Lab of Gene and Antibody Therapy, and Division of Life and Health Sciences, Graduate School at Shenzhen, Tsinghua University, Shenzhen, P.R. China; School of Life Sciences, Tsinghua University, Beijing, P.R. China.
authors.forename: Yilin
authors.initials: Y
authors.lastname: Wang
authors.affiliation: The Shenzhen Key Lab of Gene and Antibody Therapy, and Division of Life and Health Sciences, Graduate School at Shenzhen, Tsinghua University, Shenzhen, P.R. China; School of Life Sciences, Tsinghua University, Beijing, P.R. China.
authors.forename: Houjie
authors.initials: H
authors.lastname: Chen
authors.affiliation: The Shenzhen Key Lab of Gene and Antibody Therapy, and Division of Life and Health Sciences, Graduate School at Shenzhen, Tsinghua University, Shenzhen, P.R. China; School of Life Sciences, Tsinghua University, Beijing, P.R. China.
authors.forename: Teng
authors.initials: T
authors.lastname: Wang
authors.affiliation: The Shenzhen Key Lab of Gene and Antibody Therapy, and Division of Life and Health Sciences, Graduate School at Shenzhen, Tsinghua University, Shenzhen, P.R. China.
authors.forename: Hsiang-I
authors.initials: HI
authors.lastname: Tsai
authors.affiliation: The Shenzhen Key Lab of Gene and Antibody Therapy, and Division of Life and Health Sciences, Graduate School at Shenzhen, Tsinghua University, Shenzhen, P.R. China; School of Life Sciences, Tsinghua University, Beijing, P.R. China.
authors.forename: Ran
authors.initials: R
authors.lastname: Zhang
authors.affiliation: The Shenzhen Key Lab of Gene and Antibody Therapy, and Division of Life and Health Sciences, Graduate School at Shenzhen, Tsinghua University, Shenzhen, P.R. China; School of Life Sciences, Tsinghua University, Beijing, P.R. China.
authors.forename: Danfeng
authors.initials: D
authors.lastname: Chang
authors.affiliation: The Shenzhen Key Lab of Gene and Antibody Therapy, and Division of Life and Health Sciences, Graduate School at Shenzhen, Tsinghua University, Shenzhen, P.R. China; School of Life Sciences, Tsinghua University, Beijing, P.R. China.
authors.forename: Shuai
authors.initials: S
authors.lastname: He
authors.affiliation: The Shenzhen Key Lab of Gene and Antibody Therapy, and Division of Life and Health Sciences, Graduate School at Shenzhen, Tsinghua University, Shenzhen, P.R. China; School of Life Sciences, Tsinghua University, Beijing, P.R. China. Electronic address: mei.lin@sz.tsinghua.edu.cn.
authors.forename: Lin
authors.initials: L
authors.lastname: Mei
authors.affiliation: The Shenzhen Key Lab of Gene and Antibody Therapy, and Division of Life and Health Sciences, Graduate School at Shenzhen, Tsinghua University, Shenzhen, P.R. China; School of Life Sciences, Tsinghua University, Beijing, P.R. China. Electronic address: shixj@sz.tsinghua.edu.cn.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Shi
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000627:therapeutic use*; D002460:Cell Line; D004337:Drug Carriers / Q000737:chemistry; D005260:Female; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects / Q000473:pathology / Q000528:radiation effects; D019344:Lactic Acid / Q000737:chemistry*; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011794:Quercetin / Q000008:administration & dosage / Q000627:therapeutic use*; D011833:Radiation Injuries, Experimental / Q000188:drug therapy / Q000209:etiology / Q000473:pathology; D012867:Skin / Q000187:drug effects* / Q000473:pathology / Q000528:radiation effects*; D012871:Skin Diseases / Q000188:drug therapy* / Q000209:etiology / Q000473:pathology; D014466:Ultraviolet Rays / Q000009:adverse effects*; D014810:Vitamin E / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D004337:Drug Carriers; D014810:Vitamin E; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D011794:Quercetin; C014225:tocophersolan
keywords: Anti-ultraviolet (UV) radiation; Nanomedicine; PLGA-TPGS; Quercetin; Skin disease