title: Patterning hypoxic multicellular spheroids in a 3D matrix - a promising method for anti-tumor drug screening.
abstract: 3D multicellular spheroid models are of great value in the investigation of tumor biology and tumor responses to chemotherapy and radiation. To establish a mimicking tumor microenvironment in vitro, we developed a straightforward method by patterning hypoxic multicellular spheroids in a 3D matrix. The efficacy of this approach was evaluated by characterizing spheroid formation, invasive capability and phenotypic transition in aggressive human glioma cells. We observed enhanced cell proliferation, spheroid formation and invasive capability in U87 glioma cells transfected with hypoxia-inducible factors (HIFs) compared with non-treated cells. We also demonstrated that the overexpression of HIFs in hypoxic glioma cells may promote cell migration by epithelial-mesenchymal transition within the 3D matrix. Compared with conventional 3D culturing techniques, the simple operation, rapid prototyping, low cost and high throughput format of the micro-patterning method facilitates the characterization of cell proliferation, migration, phenotypic function and drug evaluation in physiologically relevant 3D microenvironments. This in vitro 3D system can recapitulate the physiologically relevant tumor microenvironment and is a promising method for 3D anti-tumor drug screening and the identification of novel targets for tumor invasion and angiogenesis.
authors.affiliation: Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.|The First Affiliated Hospital of Dalian Medical University, Dalian, China.|State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang, China.
authors.forename: Jingyun
authors.initials: J
authors.lastname: Ma
authors.affiliation: Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang, China.
authors.forename: Haibo
authors.initials: H
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang, China.
authors.forename: Lianqing
authors.initials: L
authors.lastname: Liu
authors.affiliation: Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Shi
authors.affiliation: Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.
authors.forename: Yanfeng
authors.initials: Y
authors.lastname: Li
authors.affiliation: Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China. jhqin@dicp.ac.cn.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Qin
mesh_terms: D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics*; D015687:Cell Hypoxia; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D046508:Culture Techniques / Q000379:methods*; D004354:Drug Screening Assays, Antitumor; D058750:Epithelial-Mesenchymal Transition; D005910:Glioma / Q000235:genetics* / Q000473:pathology*; D006801:Humans; D018874:Spheroids, Cellular / Q000166:cytology / Q000473:pathology*; D059016:Tumor Microenvironment
chemical_list: D051792:Basic Helix-Loop-Helix Proteins
keywords: Drug screening; Hypoxia-inducible factors (HIFs); Micro-patterning; Multicellular spheroids; Phenotype transition