title: Association between MDM2 SNP309 T>G polymorphism and the risk of bladder cancer: new data in a Chinese population and an updated meta-analysis.
abstract: OBJECTIVE
  Human murine double minute 2 protein (MDM2) is mainly a negative regulator of p53 tumor suppressor pathway. We aimed to investigate the association between MDM2 SNP309 polymorphism and bladder cancer risk.
  
  
  METHODS
  A total of 535 bladder cancer patients and 649 health controls were recruited for our study. MDM2 SNP309 T>G polymorphism was genotyped by polymerase chain reaction-ligase detection reaction method. Logistic regression was used to analyze the relationship between the genotype and susceptibility of bladder cancer. Kaplan-Meier estimates and log-rank test were obtained to analyze the association between the genotype and risk of recrudesce in nonmuscle-invasive bladder cancer patients. A multivariable Cox proportional hazards model was fitted to identify independent prognostic factors. To further investigate the association, we conducted a meta-analysis including six studies.
  
  
  RESULTS
  The frequency of the MDM2 SNP309 T>G polymorphism showed no significant difference between cases and controls (all P>0.05). In the stratification analysis, the results showed that G allele carriers were prone to have a significant decrease in risk of low-grade bladder cancer (adjusted odds ratio: 0.613, 95% confidence interval: 0.427-0.881), and G variant was associated with a significantly reduced risk of recurrence in nonmuscle-invasive bladder cancer patients with or without chemotherapy (P<0.05). The results of the meta-analysis showed that G allele and GG genotype of MDM2 SNP309 polymorphism were significantly associated with increased risk of bladder cancer in Caucasians (both P<0.05), and no association was observed in total populations and Asians (P>0.05).
  
  
  CONCLUSIONS
  MDM2 SNP309 T>G polymorphism has no influence on bladder cancer risk in Asians, but this single nucleotide polymorphism may be associated with genetic susceptibility of bladder cancer among Caucasians.
authors.affiliation: Department of Urology, The Second Hospital of Tianjin Medical University, Tianjin, People's Republic of China ; Tianjin Key Laboratory of Urology, Tianjin Institute of Urology, Tianjin, People's Republic of China.
authors.forename: Linguo
authors.initials: L
authors.lastname: Xie
authors.affiliation: Tianjin Key Laboratory of Urology, Tianjin Institute of Urology, Tianjin, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Department of Urology, The Second Hospital of Tianjin Medical University, Tianjin, People's Republic of China ; Tianjin Key Laboratory of Urology, Tianjin Institute of Urology, Tianjin, People's Republic of China.
authors.forename: Tao
authors.initials: T
authors.lastname: Chen
authors.affiliation: Department of Urology, The Second Hospital of Tianjin Medical University, Tianjin, People's Republic of China ; Tianjin Key Laboratory of Urology, Tianjin Institute of Urology, Tianjin, People's Republic of China.
authors.forename: Dawei
authors.initials: D
authors.lastname: Tian
authors.affiliation: Department of Dermatology, The Second Hospital of Tianjin Medical University, Tianjin, People's Republic of China.
authors.forename: Yujuan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Urology, The Second Hospital of Tianjin Medical University, Tianjin, People's Republic of China ; Tianjin Key Laboratory of Urology, Tianjin Institute of Urology, Tianjin, People's Republic of China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Tianjin Key Laboratory of Urology, Tianjin Institute of Urology, Tianjin, People's Republic of China.
authors.forename: Na
authors.initials: N
authors.lastname: Ding
authors.affiliation: Department of Urology, The Second Hospital of Tianjin Medical University, Tianjin, People's Republic of China ; Tianjin Key Laboratory of Urology, Tianjin Institute of Urology, Tianjin, People's Republic of China.
authors.forename: Zhonghua
authors.initials: Z
authors.lastname: Shen
authors.affiliation: Department of Urology, The Second Hospital of Tianjin Medical University, Tianjin, People's Republic of China ; Tianjin Key Laboratory of Urology, Tianjin Institute of Urology, Tianjin, People's Republic of China.
authors.forename: Hao
authors.initials: H
authors.lastname: Xu
authors.affiliation: Department of Urology, Tianjin Nankai Hospital, Tianjin, People's Republic of China.
authors.forename: Xuewu
authors.initials: X
authors.lastname: Nian
authors.affiliation: Department of Urology, The Second Hospital of Tianjin Medical University, Tianjin, People's Republic of China ; Tianjin Key Laboratory of Urology, Tianjin Institute of Urology, Tianjin, People's Republic of China.
authors.forename: Nan
authors.initials: N
authors.lastname: Sha
authors.affiliation: Department of Urology, The Second Hospital of Tianjin Medical University, Tianjin, People's Republic of China ; Tianjin Key Laboratory of Urology, Tianjin Institute of Urology, Tianjin, People's Republic of China.
authors.forename: Ruifa
authors.initials: R
authors.lastname: Han
authors.affiliation: Department of Urology, The Second Hospital of Tianjin Medical University, Tianjin, People's Republic of China ; Tianjin Key Laboratory of Urology, Tianjin Institute of Urology, Tianjin, People's Republic of China.
authors.forename: Hailong
authors.initials: H
authors.lastname: Hu
authors.affiliation: Department of Urology, The Second Hospital of Tianjin Medical University, Tianjin, People's Republic of China ; Tianjin Key Laboratory of Urology, Tianjin Institute of Urology, Tianjin, People's Republic of China.
authors.forename: Changli
authors.initials: C
authors.lastname: Wu
keywords: MDM2; bladder cancer; meta-analysis; recurrence; rs2279744; single nucleotide polymorphism