title: The impact of the IL-1β, IL-1Ra, IL-2, IL-6 and IL-10 gene polymorphisms on the development of Behcet's disease and their association with the phenotype.
abstract: OBJECTIVE
  This trial was designed to investigate the effects of the interleukin (IL)-1β, IL-1Ra, IL-2, IL-6, IL-10 gene polymorphisms on Behcet's disease (BD) occurrence and the association between the polymorphisms and the phenotype.
  
  
  METHODS
  The study population consisted of 71 patients and 70 age and gender-matched healthy subjects. Each of the participants had 2cc of blood withdrawn, which was placed into a whole blood tube, and the DNA was obtained using the NucleoSpin(®) Blood DNA Isolation kit. To display the band lengths, the products were amplified using the primary pairs of the interleukins investigated and developed in a 2% agarose gel.
  
  
  RESULTS
  There were no significant differences between the groups with respect to the IL-1Ra, IL-1β, IL-2, IL-6 and the IL-10 gene polymorphism distributions. In the patient group the IL-1RN2 gene polymorphism was detected to be statistically correlated with the presence of articular involvement (p=0.0283) and the IL-1β gene polymorphism was statistically correlated with the presence of an ocular lesion (p=0.0178). The evaluation of the IL-2 gene polymorphism (p=0.0065) and IL-10 gene polymorphism (p=0.0483) distributions with respect to age of BD onset revealed a statistically significant distribution.
  
  
  CONCLUSIONS
  The statistical correlations between the articular involvement and IL-1RN, the ocular involvement and the IL-1β, and the age of disease onset and the IL-2 and IL-10 gene polymorphisms, detected for the first time in the literature, suggest that these polymorphisms could be statistically associated with the disease symptoms and used as prognostic factors.
authors.affiliation: Department of Medical Genetics, Mevlana University Faculty of Medicine, Konya, Turkey.
authors.forename: Savaş
authors.initials: S
authors.lastname: Barış
authors.affiliation: Department of Internal Medicine, Mevlana University Faculty of Medicine, Konya, Turkey. Electronic address: dromerakyurek@gmail.com.
authors.forename: Ömer
authors.initials: Ö
authors.lastname: Akyürek
authors.affiliation: Department of Medical Genetics, Zonguldak Karaelmas University Medical Faculty Training and Research Hospital, Zonguldak, Turkey.
authors.forename: Ahmet
authors.initials: A
authors.lastname: Dursun
authors.affiliation: Department of Biostatistics, Yıldırım Beyazıt University, Ankara, Turkey.
authors.forename: Mesut
authors.initials: M
authors.lastname: Akyol
mesh_terms: D000293:Adolescent; D000328:Adult; D001528:Behcet Syndrome / Q000175:diagnosis / Q000235:genetics* / Q000628:therapy; D016022:Case-Control Studies; D005260:Female; D005500:Follow-Up Studies; D005819:Genetic Markers; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D053590:Interleukin 1 Receptor Antagonist Protein / Q000235:genetics; D016753:Interleukin-10 / Q000235:genetics; D053583:Interleukin-1beta / Q000235:genetics; D007376:Interleukin-2 / Q000235:genetics; D015850:Interleukin-6 / Q000235:genetics; D007378:Interleukins / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D011379:Prognosis; D055815:Young Adult
chemical_list: D005819:Genetic Markers; C508609:IL10 protein, human; C508585:IL1B protein, human; C507763:IL1RN protein, human; C508594:IL2 protein, human; C508600:IL6 protein, human; D053590:Interleukin 1 Receptor Antagonist Protein; D053583:Interleukin-1beta; D007376:Interleukin-2; D015850:Interleukin-6; D007378:Interleukins; D016753:Interleukin-10
keywords: Behcet's disease; Enfermedad de Behcet; IL-1Ra; IL-1β; IL-2; IL-6 and IL-10; IL-6 e IL-10