title: Direct Lipido-Metabolomics of Single Floating Cells for Analysis of Circulating Tumor Cells by Live Single-cell Mass Spectrometry.
abstract: Direct trapping of a single floating cell, i.e. a white blood cell from a drop of blood, within a nanospray tip was followed by super-sonication after the addition of ionization solvent. Molecular detection of an increased number of peaks with a higher intensity and a wider m/z range, which extends from metabolites to lipids, was acquired than of that without sonication. This method was applied to a few separated circulating tumor cells (CTC) from a neuroblastoma patient's blood to obtain their lipido-metabolomic molecular profile at the single cell level. In addition to vital molecules such as amino acids, catechol amine metabolites, which are specific to neuroblastoma, and drugs included in the patient's course of therapy were detected. This established "direct single-cell lipido-metabolomic method" seems to be useful for direct and wide range molecular detection not only for many live single-cells, but also for rare cells, such as CTCs, for future molecular diagnosis.
authors.affiliation: Natural Science Center for Basic Research and Development, Hiroshima University.
authors.forename: Eiso
authors.initials: E
authors.lastname: Hiyama
authors.forename: Ahmed
authors.initials: A
authors.lastname: Ali
authors.forename: Sara
authors.initials: S
authors.lastname: Amer
authors.forename: Takanori
authors.initials: T
authors.lastname: Harada
authors.forename: Kazumi
authors.initials: K
authors.lastname: Shimamoto
authors.forename: Rie
authors.initials: R
authors.lastname: Furushima
authors.forename: Yasmine
authors.initials: Y
authors.lastname: Abouleila
authors.forename: Samy
authors.initials: S
authors.lastname: Emara
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Masujima
mesh_terms: D000008:Abdominal Neoplasms / Q000097:blood / Q000378:metabolism; D002648:Child; D006801:Humans; D007962:Leukocytes / Q000378:metabolism*; D050356:Lipid Metabolism; D008297:Male; D055432:Metabolomics / Q000295:instrumentation / Q000379:methods*; D009360:Neoplastic Cells, Circulating / Q000378:metabolism*; D009447:Neuroblastoma / Q000097:blood / Q000378:metabolism; D059010:Single-Cell Analysis / Q000295:instrumentation / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*