title: Extract of Ziziphus jujuba Fruit (Jujube) Stimulates Expression of Enzymes Responsible for Heme Recycle via Anti-oxidant Response Element in Cultured Murine Macrophages.
abstract: Jujube, the fruit of Ziziphus jujuba Mill., is a functional food and commonly used as a health supplement worldwide. To study the beneficial role of jujube in heme iron recycling during erythrophagocytosis, the expression of heme oxygenase-1 (HO-1), biliverdin reductase A and B, and ferroportin were determined in jujube-treated cultured RAW 264.7 macrophages. Application of a chemically standardized jujube water extract in cultured RAW 264.7 cells for 24 h stimulated the expressions of HO-1, biliverdin reductase A, biliverdin reductase B, and ferroportin in a concentration-dependent manner, having the maximal responses from twofolds to threefolds. A plasmid containing anti-oxidant response element, a regulator for HO-1 transcription, was transfected into RAW 264.7 cells. Application of jujube water extract onto the transfected macrophages stimulated the anti-oxidant response element-mediated transcriptional activity by twofolds. These results supported the potential capacity of jujube by regulating expressions of heme iron recycling genes in cultured macrophages.
authors.affiliation: Pharmaceutical Department, Shenzhen Traditional Chinese Medicine Hospital, Guangzhou University of Chinese Medicine, Shenzhen, China.|Division of Life Science and Center for Chinese Medicine, The Hong Kong University of Science and Technology, Hong Kong, China.
authors.forename: Jianping
authors.initials: J
authors.lastname: Chen
authors.affiliation: Division of Life Science and Center for Chinese Medicine, The Hong Kong University of Science and Technology, Hong Kong, China.
authors.forename: Candy T W
authors.initials: CT
authors.lastname: Lam
authors.affiliation: Pharmaceutical Department, Shenzhen Traditional Chinese Medicine Hospital, Guangzhou University of Chinese Medicine, Shenzhen, China.
authors.forename: Zhonggui
authors.initials: Z
authors.lastname: Li
authors.affiliation: Division of Life Science and Center for Chinese Medicine, The Hong Kong University of Science and Technology, Hong Kong, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Yao
authors.affiliation: Division of Life Science and Center for Chinese Medicine, The Hong Kong University of Science and Technology, Hong Kong, China.
authors.forename: Huangquan
authors.initials: H
authors.lastname: Lin
authors.affiliation: Division of Life Science and Center for Chinese Medicine, The Hong Kong University of Science and Technology, Hong Kong, China.
authors.forename: Tina T X
authors.initials: TT
authors.lastname: Dong
authors.affiliation: Division of Life Science and Center for Chinese Medicine, The Hong Kong University of Science and Technology, Hong Kong, China.
authors.forename: Karl W K
authors.initials: KW
authors.lastname: Tsim
mesh_terms: D000818:Animals; D061745:Antioxidant Response Elements; D027682:Cation Transport Proteins / Q000378:metabolism; D057899:Cytophagocytosis; D005638:Fruit / Q000737:chemistry; D006418:Heme / Q000378:metabolism*; D051547:Heme Oxygenase-1 / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects / Q000201:enzymology*; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D044925:Oxidoreductases Acting on CH-CH Group Donors / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D010957:Plasmids; D000067996:RAW 264.7 Cells; D014162:Transfection; D031957:Ziziphus / Q000737:chemistry*; D000098742:Ferroportin
chemical_list: D027682:Cation Transport Proteins; D008565:Membrane Proteins; D010936:Plant Extracts; D000098742:Ferroportin; D006418:Heme; D051547:Heme Oxygenase-1; C496542:Hmox1 protein, mouse; D044925:Oxidoreductases Acting on CH-CH Group Donors; C019789:biliverdin reductase
keywords: Rhamnaceae; Ziziphus jujuba; anti-oxidant response element; heme iron recycling; heme oxygenase-1