title: Antioxidant nutrients in plasma of Japanese patients with chronic obstructive pulmonary disease, asthma-COPD overlap syndrome and bronchial asthma.
abstract: BACKGROUND
  Few studies to date have investigated the antioxidant nutrients such as vitamin C (ascorbic acid), vitamin E (α-tocopherol), retinol and carotenoids in plasma from patients with pulmonary disease in Japan. To clarify the role of antioxidant nutrients such as vitamin C, vitamin E, retinol and various carotenoids in plasma of Japanese patients with chronic obstructive lung diseases (COPD), asthma-COPD overlap syndrome (ACOS) and/or bronchial asthma (BA), we compared to healthy elderly controls.
  
  
  METHODS
  Ascorbic acid (AA), carotenoids (lutein, zeaxanthin, β-cryptoxanthin, α-carotene, β-carotene and lycopene), retinol and α-tocopherol levels in plasma were determined by using a high performance liquid chromatography. Reduced glutathione (GSH), oxidised glutathione (GSSG) in whole blood and urinary 8-OHdG were also determined.
  
  
  RESULTS
  Plasma AA level of COPD subjects was significantly lower than that of healthy elderly people. Conversely, ACOS and BA subjects showed no significant difference from healthy elderly people. Moreover, plasma lycopene and total carotenoid levels and GSH content in blood were significantly lower in COPD subjects than these in healthy elderly people. However, other redox markers such as GSSG, GSH/GSSG ratio and urinary 8-OHdG found no significant differences between COPD, ACOS and BA compared to healthy elderly people.
  
  
  CONCLUSIONS
  These results suggested that COPD of Japanese patients may develop partly because of oxidative stress derived from a shortage of antioxidant nutrients, especially of AA and lycopene, as well as GSH while this may not be the case in both ACOS and BA.
authors.affiliation: Division of Respiratory Medicine, Juntendo University Faculty of Medicine & Graduate School of Medicine, Tokyo, Japan.
authors.forename: Yuzo
authors.initials: Y
authors.lastname: Kodama
authors.affiliation: Molecular Regulation of Aging, Tokyo Metropolitan Institute of Gerontology, Tokyo, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Kishimoto
authors.affiliation: Department of Respiratory Medicine, Toho University Omori Medical Center, Tokyo, Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Muramatsu
authors.affiliation: Department of Laboratory Medicine, Toho University Faculty of Medicine, Tokyo, Japan.
authors.forename: Junko
authors.initials: J
authors.lastname: Tatebe
authors.affiliation: Research & Development Division, Kagome, Nasu-gun, Tochigi, Japan.
authors.forename: Yu
authors.initials: Y
authors.lastname: Yamamoto
authors.affiliation: Department of Respiratory Medicine, Toho University Omori Medical Center, Tokyo, Japan.
authors.forename: Nao
authors.initials: N
authors.lastname: Hirota
authors.affiliation: Division of Respiratory Medicine, Juntendo University Faculty of Medicine & Graduate School of Medicine, Tokyo, Japan.
authors.forename: Yukinari
authors.initials: Y
authors.lastname: Itoigawa
authors.affiliation: Division of Respiratory Medicine, Juntendo University Faculty of Medicine & Graduate School of Medicine, Tokyo, Japan.
authors.forename: Ryo
authors.initials: R
authors.lastname: Atsuta
authors.affiliation: Division of Respiratory Medicine, Juntendo University Faculty of Medicine & Graduate School of Medicine, Tokyo, Japan.
authors.forename: Kengo
authors.initials: K
authors.lastname: Koike
authors.affiliation: Division of Respiratory Medicine, Juntendo University Faculty of Medicine & Graduate School of Medicine, Tokyo, Japan.
authors.forename: Tadashi
authors.initials: T
authors.lastname: Sato
authors.affiliation: Research & Development Division, Kagome, Nasu-gun, Tochigi, Japan.
authors.forename: Koich
authors.initials: K
authors.lastname: Aizawa
authors.affiliation: Division of Respiratory Medicine, Juntendo University Faculty of Medicine & Graduate School of Medicine, Tokyo, Japan.
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Takahashi
authors.affiliation: Department of Laboratory Medicine, Toho University Faculty of Medicine, Tokyo, Japan.
authors.forename: Toshisuke
authors.initials: T
authors.lastname: Morita
authors.affiliation: Department of Respiratory Medicine, Toho University Omori Medical Center, Tokyo, Japan.
authors.forename: Sakae
authors.initials: S
authors.lastname: Homma
authors.affiliation: Division of Respiratory Medicine, Juntendo University Faculty of Medicine & Graduate School of Medicine, Tokyo, Japan.
authors.forename: Kuniaki
authors.initials: K
authors.lastname: Seyama
authors.affiliation: Molecular Regulation of Aging, Tokyo Metropolitan Institute of Gerontology, Tokyo, Japan.
authors.forename: Akihito
authors.initials: A
authors.lastname: Ishigami
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000975:Antioxidants / Q000032:analysis*; D001205:Ascorbic Acid / Q000097:blood; D001249:Asthma / Q000097:blood / Q000503:physiopathology* / Q000652:urine; D015415:Biomarkers / Q000097:blood*; D002338:Carotenoids / Q000097:blood; D002853:Chromatography, Liquid / Q000379:methods; D005260:Female; D005502:Food; D005978:Glutathione / Q000097:blood / Q000378:metabolism / Q000652:urine; D006801:Humans; D007564:Japan / Q000453:epidemiology; D000077276:Lycopene; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress / Q000502:physiology; D029424:Pulmonary Disease, Chronic Obstructive / Q000097:blood / Q000503:physiopathology* / Q000652:urine; D012129:Respiratory Function Tests / Q000379:methods; D012907:Smoking / Q000009:adverse effects
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D002338:Carotenoids; D005978:Glutathione; D001205:Ascorbic Acid; D000077276:Lycopene
keywords: ascorbic acid; asthma-COPD overlap syndrome; bronchial asthma; carotenoid; chronic obstructive pulmonary disease; lycopene