title: Effect of nicotinic acetylcholine receptor alpha 1 (nAChRα1) peptides on rabies virus infection in neuronal cells.
abstract: Rabies virus (RABV) is neurotropic and causes acute progressive encephalitis. Herein, we report the interaction of nAChRα1-subunit peptides with RABV and the effect of these peptides on RABV infection in cultured neuronal cells. Peptide sequences derived from torpedo, bovine, human and rats were synthesized and studied for their interactions with RABV using virus capture ELISA and peptide immunofluorescence. The results showed specific binding of the nAChRα1-subunit peptides to the RABV. In the virus adsorption assay, these peptides were found to inhibit the attachment of the RABV to the neuronal cells. The nAChRα1-subunit peptides inhibited the RABV infection and reduced viral gene expression in the cultured neuroblastoma (N2A) cells. Torpedo peptide sequence (T-32) had highest antiviral effect (IC50=14±3.01μM) compared to the other peptides studied. The results of the study indicated that nAChRα1-subunit peptides may act as receptor decoy molecules and inhibit the binding of virus to the native host cell receptors and hence may reduce viral infection.
authors.affiliation: Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Bareilly 243122, Uttar Pradesh, India.
authors.forename: Basavaraj
authors.initials: B
authors.lastname: Sajjanar
authors.affiliation: Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Bareilly 243122, Uttar Pradesh, India.
authors.forename: Shikha
authors.initials: S
authors.lastname: Saxena
authors.affiliation: Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Bareilly 243122, Uttar Pradesh, India.
authors.forename: Deepika
authors.initials: D
authors.lastname: Bisht
authors.affiliation: Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Bareilly 243122, Uttar Pradesh, India.
authors.forename: Arvind Kumar
authors.initials: AK
authors.lastname: Singh
authors.affiliation: National Institute of Veterinary Epidemiology and Disease Informatics, Bengaluru 560064, Karnataka, India.
authors.forename: G B
authors.initials: GB
authors.lastname: Manjunatha Reddy
authors.affiliation: Division of Veterinary Pathology, Indian Veterinary Research Institute, Bareilly 243122, Uttar Pradesh, India.
authors.forename: Rajendra
authors.initials: R
authors.lastname: Singh
authors.affiliation: Division of Biological Products, Indian Veterinary Research Institute, Bareilly 243122, Uttar Pradesh, India.
authors.forename: R P
authors.initials: RP
authors.lastname: Singh
authors.affiliation: Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Bareilly 243122, Uttar Pradesh, India. Electronic address: drsatishkumar_ivri@yahoo.co.in.
authors.forename: Satish
authors.initials: S
authors.lastname: Kumar
mesh_terms: D000818:Animals; D002417:Cattle; D045744:Cell Line, Tumor; D006801:Humans; D051379:Mice; D009474:Neurons / Q000378:metabolism* / Q000821:virology*; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000378:metabolism*; D011818:Rabies / Q000378:metabolism*; D011820:Rabies virus / Q000378:metabolism*; D051381:Rats; D011978:Receptors, Nicotinic / Q000378:metabolism*; D014101:Torpedo
chemical_list: C523021:CHRNA1 protein, human; D010446:Peptide Fragments; D011978:Receptors, Nicotinic
keywords: Neuroblastoma cells; Peptides; Rabies virus; Receptor subunits