title: Regulatory role of the cell adhesion molecule nectin-1 in GABAergic inhibitory synaptic transmission in the CA3 region of mouse hippocampus.
abstract: Proper operation of a neural circuit relies on both excitatory and inhibitory synapses. We previously showed that cell adhesion molecules nectin-1 and nectin-3 are localized at puncta adherentia junctions of the hippocampal mossy fiber glutamatergic excitatory synapses and that they do not regulate the excitatory synaptic transmission onto the CA3 pyramidal cells. We studied here the roles of these nectins in the GABAergic inhibitory synaptic transmission onto the CA3 pyramidal cells using nectin-1-deficient and nectin-3-deficient cultured mouse hippocampal slices. In these mutant slices, the amplitudes and frequencies of miniature excitatory postsynaptic currents were indistinguishable from those in the control slices. In the nectin-1-deficient slices, but not in the nectin-3-deficient slices, however, the amplitude of miniature inhibitory postsynaptic currents (mIPSCs) was larger than that in the control slices, although the frequency of the mIPSCs was not different between these two groups of slices. In the dissociated culture of hippocampal neurons from the nectin-1-deficient mice, the amplitude and frequency of mIPSCs were indistinguishable from those in the control neurons. Nectin-1 was not localized at or near the GABAergic inhibitory synapses. These results indicate that nectin-1 regulates the neuronal activities in the CA3 region of the hippocampus by suppressing the GABAergic inhibitory synaptic transmission.
authors.affiliation: Faculty of Health Sciences, Kobe University Graduate School of Health Sciences, Kobe, Hyogo, 654-0142, Japan.|Division of Neurophysiology, Department of Cellular Physiology, Kobe University Graduate School of Medicine, Kobe, Hyogo, 650-0017, Japan.|CREST, Japan Science and Technology Agency, Kobe, Hyogo, 650-0047, Japan.
authors.forename: Xiaoqi
authors.initials: X
authors.lastname: Geng
authors.affiliation: CREST, Japan Science and Technology Agency, Kobe, Hyogo, 650-0047, Japan.|Division of Pathogenetic Signaling, Department of Biochemistry and Molecular Biology, Kobe University Graduate School of Medicine, Kobe, Hyogo, 650-0047, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Mandai
authors.affiliation: CREST, Japan Science and Technology Agency, Kobe, Hyogo, 650-0047, Japan.|Division of Pathogenetic Signaling, Department of Biochemistry and Molecular Biology, Kobe University Graduate School of Medicine, Kobe, Hyogo, 650-0047, Japan.
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Maruo
authors.affiliation: CREST, Japan Science and Technology Agency, Kobe, Hyogo, 650-0047, Japan.|Department of Neural Regeneration and Cell Communication, Mie University Graduate School of Medicine, Tsu, Mie, 514-8507, Japan.
authors.forename: Shujie
authors.initials: S
authors.lastname: Wang
authors.affiliation: CREST, Japan Science and Technology Agency, Kobe, Hyogo, 650-0047, Japan.|Department of Neural Regeneration and Cell Communication, Mie University Graduate School of Medicine, Tsu, Mie, 514-8507, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Fujiwara
authors.affiliation: CREST, Japan Science and Technology Agency, Kobe, Hyogo, 650-0047, Japan.|Department of Neural Regeneration and Cell Communication, Mie University Graduate School of Medicine, Tsu, Mie, 514-8507, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Mizoguchi
authors.affiliation: CREST, Japan Science and Technology Agency, Kobe, Hyogo, 650-0047, Japan.|Division of Pathogenetic Signaling, Department of Biochemistry and Molecular Biology, Kobe University Graduate School of Medicine, Kobe, Hyogo, 650-0047, Japan.
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Takai
authors.affiliation: Faculty of Health Sciences, Kobe University Graduate School of Health Sciences, Kobe, Hyogo, 654-0142, Japan.|Division of Neurophysiology, Department of Cellular Physiology, Kobe University Graduate School of Medicine, Kobe, Hyogo, 650-0017, Japan.|CREST, Japan Science and Technology Agency, Kobe, Hyogo, 650-0047, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Mori
mesh_terms: D000818:Animals; D056654:CA3 Region, Hippocampal / Q000378:metabolism* / Q000648:ultrastructure; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D002478:Cells, Cultured; D019706:Excitatory Postsynaptic Potentials; D059330:GABAergic Neurons / Q000378:metabolism* / Q000648:ultrastructure; D005971:Glutamates / Q000378:metabolism; D053444:Inhibitory Postsynaptic Potentials; D051379:Mice; D000075983:Nectins; D017966:Pyramidal Cells / Q000378:metabolism; D013569:Synapses / Q000378:metabolism / Q000648:ultrastructure; D009435:Synaptic Transmission
chemical_list: D015815:Cell Adhesion Molecules; D005971:Glutamates; C000619015:Nectin1 protein, mouse; C000619017:Nectin3 protein, mouse; D000075983:Nectins