title: In vitro evaluation of decellularized ECM-derived surgical scaffold biomaterials.
abstract: Decellularized extracellular matrix (ECM) biomaterials are increasingly used in regenerative medicine for abdominal tissue repair. Emerging ECM biomaterials with greater compliance target surgical procedures like breast and craniofacial reconstruction to enhance aesthetic outcome. Clinical studies report improved outcomes with newly designed ECM scaffolds, but their comparative biological characteristics have received less attention. In this study, we investigated scaffolds derived from dermis (AlloDerm Regenerative Tissue Matrix), small intestinal submucosa (Surgisis 4-layer Tissue Graft and OASIS Wound Matrix), and mesothelium (Meso BioMatrix Surgical Mesh and Veritas Collagen Matrix) and evaluated biological properties that modulate cellular responses and recruitment. An assay panel was utilized to assess the ECM scaffold effects upon cells. Results of the material-conditioned media study demonstrated Meso BioMatrix and OASIS best supported cell proliferation. Meso BioMatrix promoted the greatest migration and chemotaxis signaling, followed by Veritas and OASIS; OASIS had superior suppression of cell apoptosis. The direct adhesion assay indicated that AlloDerm, Meso BioMatrix, Surgisis, and Veritas had sidedness that affected cell-material interactions. In the chick chorioallantoic membrane assay, Meso BioMatrix and OASIS best supported cell infiltration. Among tested materials, Meso BioMatrix and OASIS demonstrated characteristics that facilitate scaffold incorporation, making them promising choices for many clinical applications. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 585-593, 2017.
authors.affiliation: Center for Regenerative Medicine, Massachusetts General Hospital, Boston, Massachusetts, 02114.|Tongji Hospital, Huazhong University of Science and Technology, Wuhan, Hubei, 434300, People's Republic of China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Luo
authors.affiliation: Center for Regenerative Medicine, Massachusetts General Hospital, Boston, Massachusetts, 02114.
authors.forename: Katherine M
authors.initials: KM
authors.lastname: Kulig
authors.affiliation: Center for Regenerative Medicine, Massachusetts General Hospital, Boston, Massachusetts, 02114.|The Department of Biomedical and Chemical Engineering, Syracuse Biomaterials Institute, Syracuse University, Syracuse, New York, 13244.
authors.forename: Eric B
authors.initials: EB
authors.lastname: Finkelstein
authors.affiliation: Center for Regenerative Medicine, Massachusetts General Hospital, Boston, Massachusetts, 02114.
authors.forename: Margaret F
authors.initials: MF
authors.lastname: Nicholson
authors.affiliation: DSM Biomedical, Exton, Pennysylvania, 19341.
authors.forename: Xiang-Hong
authors.initials: XH
authors.lastname: Liu
authors.affiliation: DSM Biomedical, Exton, Pennysylvania, 19341.
authors.forename: Scott M
authors.initials: SM
authors.lastname: Goldman
authors.affiliation: Department of Surgery, Massachusetts General Hospital, Boston, Massachusetts, 02114.|Harvard Medical School, Boston, Massachusetts, 02115.
authors.forename: Joseph P
authors.initials: JP
authors.lastname: Vacanti
authors.affiliation: Harvard Medical School, Boston, Massachusetts, 02115.|Department of Orthopaedic Surgery, Massachusetts General Hospital, Boston, Massachusetts, 02114.
authors.forename: Brian E
authors.initials: BE
authors.lastname: Grottkau
authors.affiliation: Center for Regenerative Medicine, Massachusetts General Hospital, Boston, Massachusetts, 02114.|Department of Surgery, Massachusetts General Hospital, Boston, Massachusetts, 02114.|Harvard Medical School, Boston, Massachusetts, 02115.
authors.forename: Irina
authors.initials: I
authors.lastname: Pomerantseva
authors.affiliation: Center for Regenerative Medicine, Massachusetts General Hospital, Boston, Massachusetts, 02114.|Department of Surgery, Massachusetts General Hospital, Boston, Massachusetts, 02114.|Harvard Medical School, Boston, Massachusetts, 02115.
authors.forename: Cathryn A
authors.initials: CA
authors.lastname: Sundback
authors.affiliation: Center for Regenerative Medicine, Massachusetts General Hospital, Boston, Massachusetts, 02114.|Department of Surgery, Massachusetts General Hospital, Boston, Massachusetts, 02114.|Harvard Medical School, Boston, Massachusetts, 02115.|Department of Orthopaedic Surgery, Massachusetts General Hospital, Boston, Massachusetts, 02114.
authors.forename: Craig M
authors.initials: CM
authors.lastname: Neville
mesh_terms: D000818:Animals; D017209:Apoptosis; D002417:Cattle; D049109:Cell Proliferation; D002633:Chemotaxis; D020405:Dermis / Q000737:chemistry*; D005109:Extracellular Matrix / Q000737:chemistry*; D005347:Fibroblasts / Q000378:metabolism*; D006801:Humans; D051379:Mice; D041681:NIH 3T3 Cells; D015398:Signal Transduction; D013552:Swine; D054457:Tissue Scaffolds / Q000737:chemistry*
keywords: cell adhesion; cell proliferation; extracellular matrix; in vitro; scaffolds