title: HPLC Method for Chemical Fingerprinting of Guggul (Commiphora wightii)--Quantification of E- and Z-Guggulsterones and Detection of Possible Adulterants.
abstract: A reversed-phase high-performance liquid chromatography method was developed for the chemical fingerprinting of Commiphora wightii gum resin (guggul). This method was also used for the quantification of E- and Z-guggulsterones in different C. wightii gum resin samples. The analysis was conducted on a high-performance liquid chromatography system with a reversed-phase column using a gradient elution comprised of water and acetonitrile with 0.1% acetic acid, and a run time of 50 min at 38 °C. The calibration curve of E- and Z-guggulsterones showed good linearity with a standard correlation coefficient >0.999, which is within the established range (0.5-250 µg/mL). Twelve marker compounds were selected and successfully analyzed by this method in 22 C. wightii gum resin samples along with 9 gum resin samples of possible adulterant species.
authors.affiliation: National Center for Natural Products Research, University of Mississippi, University, MS, USA.
authors.forename: Rida
authors.initials: R
authors.lastname: Ahmed
authors.affiliation: National Center for Natural Products Research, University of Mississippi, University, MS, USA.
authors.forename: Yan-Hong
authors.initials: YH
authors.lastname: Wang
authors.affiliation: National Center for Natural Products Research, University of Mississippi, University, MS, USA.
authors.forename: Zulfiqar
authors.initials: Z
authors.lastname: Ali
authors.affiliation: National Center for Natural Products Research, University of Mississippi, University, MS, USA.
authors.forename: Troy J
authors.initials: TJ
authors.lastname: Smillie
authors.affiliation: National Center for Natural Products Research, University of Mississippi, University, MS, USA.
authors.forename: Ikhlas A
authors.initials: IA
authors.lastname: Khan
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D029744:Commiphora / Q000737:chemistry*; D011282:Pregnenediones / Q000032:analysis*; D012116:Resins, Plant / Q000737:chemistry
chemical_list: D011282:Pregnenediones; D012116:Resins, Plant; C023617:pregna-4,17-diene-3,16-dione