title: The Saccharomyces cerevisiae poly(A) binding protein Pab1 as a target for eliciting stress tolerant phenotypes.
abstract: When exploited as cell factories, Saccharomyces cerevisiae cells are exposed to harsh environmental stresses impairing titer, yield and productivity of the fermentative processes. The development of robust strains therefore represents a pivotal challenge for the implementation of cost-effective bioprocesses. Altering master regulators of general cellular rewiring represents a possible strategy to evoke shaded potential that may accomplish the desirable features. The poly(A) binding protein Pab1, as stress granules component, was here selected as the target for obtaining widespread alterations in mRNA metabolism, resulting in stress tolerant phenotypes. Firstly, we demonstrated that the modulation of Pab1 levels improves robustness against different stressors. Secondly, the mutagenesis of PAB1 and the application of a specific screening protocol on acetic acid enriched medium allowed the isolation of the further ameliorated mutant pab1 A60-9. These findings pave the way for a novel approach to unlock industrially promising phenotypes through the modulation of a post-transcriptional regulatory element.
authors.affiliation: Department of Biotechnology and Biosciences, University of Milano-Bicocca, Milano, 20126, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Martani
authors.affiliation: Department of Biotechnology and Biosciences, University of Milano-Bicocca, Milano, 20126, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Marano
authors.affiliation: Department of Biotechnology and Biosciences, University of Milano-Bicocca, Milano, 20126, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Bertacchi
authors.affiliation: Department of Biotechnology and Biosciences, University of Milano-Bicocca, Milano, 20126, Italy.|SYSBIO - Centre of Systems Biology, Milano and Roma, Italy.
authors.forename: Danilo
authors.initials: D
authors.lastname: Porro
authors.affiliation: Department of Biotechnology and Biosciences, University of Milano-Bicocca, Milano, 20126, Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Branduardi
mesh_terms: D019342:Acetic Acid / Q000378:metabolism; D000220:Adaptation, Biological; D015870:Gene Expression; D006358:Hot Temperature; D009154:Mutation; D010641:Phenotype; D039122:Poly(A)-Binding Protein I / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D013312:Stress, Physiological / Q000235:genetics
chemical_list: D039122:Poly(A)-Binding Protein I; D012333:RNA, Messenger; D019342:Acetic Acid