title: Cloning and identification of the ASIP gene in Chinese raccoon dog (Nyctereutes procyonoides procyonoides).
abstract: The quantity, quality, and distribution of eumelanin and pheomelanin determine a wide variety of coat colors in animals. Three coat color variants exist in farmed wild-type Chinese raccoon dog (Nyctereutes procyonoides procyonoides), which is an important fur-bearing animal species. The ASIP gene is an important candidate gene for coat color variation in some species. In this study, the complete cDNA sequences of ASIP were amplified from a wild-type Chinese raccoon dog. Sequence analysis revealed the coding region of ASIP in Chinese raccoon dog to be 396-bp in length and two transcripts (accession Nos. KT224450 and KT224451) were identified due to the alternative use of exon 1 (1A and 1C). However, the alternative splicing pattern and the coding sequence of ASIP in three types of coat color variants were the same as those identified in the wild-type individual. Based on the results obtained in this study, we can exclude a role for alternative splicing of exon 1 and the coding sequence of ASIP in coat color variation in Chinese raccoon dog.
authors.affiliation: College of Animal Science, Jilin University, Changchun, China.
authors.forename: Y M
authors.initials: YM
authors.lastname: Li
authors.affiliation: College of Animal Science, Jilin University, Changchun, China.
authors.forename: S
authors.initials: S
authors.lastname: Si
authors.affiliation: College of Animal Science, Jilin University, Changchun, China.
authors.forename: P C
authors.initials: PC
authors.lastname: Guo
authors.affiliation: College of Animal Science, Jilin University, Changchun, China.
authors.forename: L L
authors.initials: LL
authors.lastname: Li
authors.affiliation: College of Animal Science, Jilin University, Changchun, China.
authors.forename: C Y
authors.initials: CY
authors.lastname: Bai
authors.affiliation: College of Animal Science, Jilin University, Changchun, China.
authors.forename: S Q
authors.initials: SQ
authors.lastname: Yan
mesh_terms: D054366:Agouti Signaling Protein / Q000737:chemistry / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D006197:Hair; D008969:Molecular Sequence Data; D010860:Pigments, Biological; D046031:Raccoon Dogs / Q000235:genetics*
chemical_list: D054366:Agouti Signaling Protein; D010860:Pigments, Biological