title: Antioxidant C-glycosylflavones of Drymaria cordata (Linn.) Willd.
abstract: A new C-glycosylflavone, drymaritin E (6-C-(3-keto-β-digitoxopyranosyl)-4'-O-(β-D-glucopyranosyl)-7-methoxyl-5,4'-dihydroxylflavone) 1 was isolated from the oily upper phase (SU) of the MeOH extract from aerial parts of Drymaria cordata together with two known compounds (cassiaoccidentalin A 2 and anemonin 3) and an inseparable mixture of two known C-glycosylflavones 5,4'-dihydroxy-7-methoxyflavone-6-C-(2''-O-α-L-rhamnopyranosyl)-β-D-glucopyranoside 4a and 5,7,3',4'-tetrahydroxyflavone-6-C-(2''-O-α-L-rhamnopyranosyl)-β-D-glucopyranoside 4b. The alkaline hydrolysis of 3 led to a new hemisynthetic derivative, sodium anemonate (sodium 2-((1'E) 2'-sodium-carboxylate-vinyl)-5-oxo-cyclohex-1-ene carboxylate) 3a. The chemical structures were determined by spectroscopic methods ((1)H NMR, (13)C NMR, (1)H-(1)H COSY, HMBC, HSQC, and NOESY) and mass spectrometry (ESI-MS). C-glycosylflavones had significant free radical-scavenging activities on the radical 2,2-diphenyl-1-picrylhydrazyl (DPPH). However, SU and compounds 3 and 3a exhibited no activity. In particular, compound 1 exhibited a concentration-dependent radical scavenging activity on DPPH with EC50 of 31.43 µg/mL.
authors.affiliation: Laboratory of Environmental and Applied Chemistry, Department of Chemistry, Faculty of Science, University of Dschang, Box 183, Dschang, Cameroon.
authors.forename: Raymond N
authors.initials: RN
authors.lastname: Nono
authors.affiliation: Laboratory of Animal Physiology and Phytopharmacology, Department of Animal Biology, Faculty of Science, University of Dschang, Box 67, Dschang, Cameroon.
authors.forename: Elvine P
authors.initials: EP
authors.lastname: Nguelefack-Mbuyo
authors.affiliation: Laboratory of Environmental and Applied Chemistry, Department of Chemistry, Faculty of Science, University of Dschang, Box 183, Dschang, Cameroon.
authors.forename: Laurence K
authors.initials: LK
authors.lastname: Nzowa
authors.affiliation: Laboratory of Environmental and Applied Chemistry, Department of Chemistry, Faculty of Science, University of Dschang, Box 183, Dschang, Cameroon.
authors.forename: Beaudelaire K
authors.initials: BK
authors.lastname: Ponou
authors.affiliation: Laboratory of Environmental and Applied Chemistry, Department of Chemistry, Faculty of Science, University of Dschang, Box 183, Dschang, Cameroon.
authors.forename: Rémy B
authors.initials: RB
authors.lastname: Teponno
authors.affiliation: Laboratory of Animal Physiology and Phytopharmacology, Department of Animal Biology, Faculty of Science, University of Dschang, Box 67, Dschang, Cameroon.
authors.forename: Télesphore B
authors.initials: TB
authors.lastname: Nguelefack
authors.affiliation: School of Science and Technology, Chemistry Division, University of Camerino, Via S. Agostino 1, 62032, Camerino, Italy.
authors.forename: Luciano
authors.initials: L
authors.lastname: Barboni
authors.affiliation: Laboratory of Environmental and Applied Chemistry, Department of Chemistry, Faculty of Science, University of Dschang, Box 183, Dschang, Cameroon. tapondjou2001@yahoo.fr.
authors.forename: Léon A
authors.initials: LA
authors.lastname: Tapondjou
authors.affiliation: Department of Pharmaceutical Engineering, Sandji University, Wonju, 220-702, Republic of Korea. hjpark@sangji.ac.kr.
authors.forename: Hee-Juhn
authors.initials: HJ
authors.lastname: Park
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D029748:Caryophyllaceae; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism; D047309:Flavones / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D008297:Male; D035261:Plant Components, Aerial; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000975:Antioxidants; C421808:C-glucosylflavone; D047309:Flavones; D005419:Flavonoids; D010936:Plant Extracts
keywords: Anemonin; Antioxidant activity; C-glycosylflavone; Caryophyllaceae; Drymaria cordata