title: The Cerato-Platanin protein Epl-1 from Trichoderma harzianum is involved in mycoparasitism, plant resistance induction and self cell wall protection.
abstract: Trichoderma harzianum species are well known as biocontrol agents against important fungal phytopathogens. Mycoparasitism is one of the strategies used by this fungus in the biocontrol process. In this work, we analyzed the effect of Epl-1 protein, previously described as plant resistance elicitor, in expression modulation of T. harzianum genes involved in mycoparasitism process against phytopathogenic fungi; self cell wall protection and recognition; host hyphae coiling and triggering expression of defense-related genes in beans plants. The results indicated that the absence of Epl-1 protein affects the expression of all mycoparasitism genes analyzed in direct confrontation assays against phytopathogen Sclerotinia sclerotiorum as well as T. harzianum itself; the host mycoparasitic coiling process and expression modulation of plant defense genes showing different pattern compared with wild type strain. These data indicated the involvement T. harzianum Epl-1 in self and host interaction and also recognition of T. harzianum as a symbiotic fungus by the bean plants.
authors.affiliation: Department of Biochemistry and Immunology, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Eriston Vieira
authors.initials: EV
authors.lastname: Gomes
authors.affiliation: Department of Biochemistry and Immunology, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Mariana do Nascimento
authors.initials: Mdo N
authors.lastname: Costa
authors.affiliation: Department of Biochemistry and Immunology, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Renato Graciano
authors.initials: RG
authors.lastname: de Paula
authors.affiliation: Department of Molecular and Cellular Biology and Pathogenic Bioagents, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Rafael Ricci
authors.initials: RR
authors.lastname: de Azevedo
authors.affiliation: Department of Cellular Biology, University of Brasilia, Brasília, Distrito Federal, Brazil.
authors.forename: Francilene Lopes
authors.initials: FL
authors.lastname: da Silva
authors.affiliation: Department of Cellular Biology, University of Brasilia, Brasília, Distrito Federal, Brazil.
authors.forename: Eliane F
authors.initials: EF
authors.lastname: Noronha
authors.affiliation: Department of Biochemistry and Cellular Biology, Biological Sciences Institute, Federal University of Goias, Goiânia, Goiás, Brazil.
authors.forename: Cirano José
authors.initials: CJ
authors.lastname: Ulhoa
authors.affiliation: Department of Biochemistry, State University of Goias, Anápolis, Goiás, Brazil.
authors.forename: Valdirene Neves
authors.initials: VN
authors.lastname: Monteiro
authors.affiliation: Department of Microbiology, University School of Agricultural Engineers, University of León, Ponferrada, Spain.
authors.forename: Rosa Elena
authors.initials: RE
authors.lastname: Cardoza
authors.affiliation: Department of Microbiology, University School of Agricultural Engineers, University of León, Ponferrada, Spain.
authors.forename: Santiago
authors.initials: S
authors.lastname: Gutiérrez
authors.affiliation: Department of Biochemistry and Immunology, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Roberto Nascimento
authors.initials: RN
authors.lastname: Silva
mesh_terms: D002473:Cell Wall / Q000378:metabolism*; D016000:Cluster Analysis; D019295:Computational Biology / Q000379:methods; D060467:Disease Resistance / Q000235:genetics*; D036382:Ephrin-A1 / Q000235:genetics* / Q000378:metabolism*; D017353:Gene Deletion; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D018506:Gene Expression Regulation, Plant; D053263:Gene Regulatory Networks; D010935:Plant Diseases / Q000382:microbiology*; D021381:Protein Transport; D022161:Transport Vesicles / Q000378:metabolism; D014242:Trichoderma / Q000502:physiology*
chemical_list: D036382:Ephrin-A1