title: Yinzhihuang attenuates ANIT-induced intrahepatic cholestasis in rats through upregulation of Mrp2 and Bsep expressions.
abstract: BACKGROUND
  The treatment of intrahepatic cholestasis has been limited, and development of an effective drug is needed. Clinical studies have shown that Yinzhihuang (YZH), a traditional Chinese decoction, enhances bilirubin clearance. The goal of this study was to determine the protective effect of YZH on experimental intrahepatic cholestasis in young rats and to explore its underlying molecular mechanisms.
  
  
  METHODS
  Intrahepatic cholestasis in rats was induced by α-naphthylisothiocyanate (ANIT) on days 1 and 8. The rats received YZH, ursodeoxycholic acid (UDCA), or vehicle for 9 d and were killed on either day 3 or day 10. Serum biomarkers, liver histology, and the distribution of protein and mRNA expression of Mrp2 and Bsep were analyzed.
  
  
  RESULTS
  YZH treatment resulted in decreased levels of serum biomarkers except γ-glutamyl transpeptidase, attenuated liver histological injuries, increased protein expressions of Mrp2 and Bsep, and upregulated expressions of Mrp2 and Bsep mRNAs. The effects of YZH on serum biomarkers (aminotransferase, alanine aminotransferase, and direct bilirubin), liver histology, and Mrp2 mRNA expressions were significantly greater and earlier than those of UDCA.
  
  
  CONCLUSIONS
  Our results suggest that YZH has protective effect against ANIT-induced intrahepatic cholestasis in rats, through upregulation of Mrp2 and Bsep expressions.
authors.affiliation: Department of Neonatology, Nanfang Hospital, Southern Medical University, Guangzhou, China.|Department of Pediatrics, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, China.
authors.forename: Qiao-Qun
authors.initials: QQ
authors.lastname: Ou
authors.affiliation: Department of Neonatology, Nanfang Hospital, Southern Medical University, Guangzhou, China.
authors.forename: Xin-Hua
authors.initials: XH
authors.lastname: Qian
authors.affiliation: Division of Pediatric Gastroenterology, Department of Pediatrics, Children's Mercy Hospital, University of Missouri Kansas City School of Medicine, Kansas City, Missouri.
authors.forename: Ding-You
authors.initials: DY
authors.lastname: Li
authors.affiliation: Department of Pediatrics, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, China.
authors.forename: You-Xiang
authors.initials: YX
authors.lastname: Zhang
authors.affiliation: Department of Neonatology, Nanfang Hospital, Southern Medical University, Guangzhou, China.
authors.forename: Xia-Nan
authors.initials: XN
authors.lastname: Pei
authors.affiliation: Department of Neonatology, Nanfang Hospital, Southern Medical University, Guangzhou, China.
authors.forename: Jin-Wen
authors.initials: JW
authors.lastname: Chen
authors.affiliation: Department of Pediatrics, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, China.
authors.forename: Li
authors.initials: L
authors.lastname: Yu
mesh_terms: D015058:1-Naphthylisothiocyanate / Q000633:toxicity*; D000074020:ATP Binding Cassette Transporter, Subfamily B, Member 11; D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002780:Cholestasis, Intrahepatic / Q000517:prevention & control*; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D008099:Liver / Q000378:metabolism; D000089762:Multidrug Resistance-Associated Protein 2; D027425:ATP-Binding Cassette, Sub-Family C Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D015854:Up-Regulation
chemical_list: D015058:1-Naphthylisothiocyanate; D000074020:ATP Binding Cassette Transporter, Subfamily B, Member 11; D018528:ATP-Binding Cassette Transporters; D015415:Biomarkers; D004365:Drugs, Chinese Herbal; D000089762:Multidrug Resistance-Associated Protein 2; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D012333:RNA, Messenger; C093491:ABCB11 protein, human; C000715087:ABCC2 protein, human; C070600:yin zhi huang