title: A lincRNA connected to cell mortality and epigenetically-silenced in most common human cancers.
abstract: Immortality is an essential characteristic of human carcinoma cells. We recently developed an efficient, reproducible method that immortalizes human mammary epithelial cells (HMEC) in the absence of gross genomic changes by targeting 2 critical senescence barriers. Consistent transcriptomic changes associated with immortality were identified using microarray analysis of isogenic normal finite pre-stasis, abnormal finite post-stasis, and immortal HMECs from 4 individuals. A total of 277 genes consistently changed in cells that transitioned from post-stasis to immortal. Gene ontology analysis of affected genes revealed biological processes significantly altered in the immortalization process. These immortalization-associated changes showed striking similarity to the gene expression changes seen in The Cancer Genome Atlas (TCGA) clinical breast cancer data. The most dramatic change in gene expression seen during the immortalization step was the downregulation of an unnamed, incompletely annotated transcript that we called MORT, for mortality, since its expression was closely associated with the mortal, finite lifespan phenotype. We show here that MORT (ZNF667-AS1) is expressed in all normal finite lifespan human cells examined to date and is lost in immortalized HMEC. MORT gene silencing at the mortal/immortal boundary was due to DNA hypermethylation of its CpG island promoter. This epigenetic silencing is also seen in human breast cancer cell lines and in a majority of human breast tumor tissues. The functional importance of DNA hypermethylation in MORT gene silencing is supported by the ability of 5-aza-2'-deoxycytidine to reactivate MORT expression. Analysis of TCGA data revealed deregulation of MORT expression due to DNA hypermethylation in 15 out of the 17 most common human cancers. The epigenetic silencing of MORT in a large majority of the common human cancers suggests a potential fundamental role in cellular immortalization during human carcinogenesis.
authors.affiliation: a Arizona Cancer Center; The University of Arizona ; Tucson , AZ USA.
authors.forename: Lukas
authors.initials: L
authors.lastname: Vrba
authors.affiliation: b Life Sciences Division; Lawrence Berkeley National Laboratory ; Berkeley , CA USA.
authors.forename: James C
authors.initials: JC
authors.lastname: Garbe
authors.affiliation: a Arizona Cancer Center; The University of Arizona ; Tucson , AZ USA.|b Life Sciences Division; Lawrence Berkeley National Laboratory ; Berkeley , CA USA.
authors.forename: Martha R
authors.initials: MR
authors.lastname: Stampfer
authors.affiliation: a Arizona Cancer Center; The University of Arizona ; Tucson , AZ USA.|c Department of Pharmacology & Toxicology ; College of Pharmacy; The University of Arizona ; Tucson , AZ USA.
authors.forename: Bernard W
authors.initials: BW
authors.lastname: Futscher
mesh_terms: D000369:Aged, 80 and over; D045744:Cell Line, Tumor; D002470:Cell Survival; D019175:DNA Methylation; D044127:Epigenesis, Genetic; D004847:Epithelial Cells; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods; D006801:Humans; D042361:Mammary Glands, Human / Q000166:cytology*; D008875:Middle Aged; D009369:Neoplasms / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D062085:RNA, Long Noncoding / Q000235:genetics*; D055815:Young Adult
chemical_list: C000611395:MORT long noncoding RNA, human; D062085:RNA, Long Noncoding
keywords: DNA methylation; HMEC; MORT; ZNF667-AS1; breast cancer; epigenetics; immortality; lincRNA; lncRNA; mammary epithelia; ncRNA