title: First comparison of adjuvant for trivalent inactivated Haemophilus parasuis serovars 4, 5 and 12 vaccines against Glässer's disease.
abstract: Haemophilus parasuis has had a huge impact in the swine industry throughout the world. Inactivated bacterium for H. parasuis is a traditional vaccine that can elicit efficient protection against homologous challenges. The objective of this study was to screen for the adjuvant-enhanced immune effect of trivalent inactivated H. parasuis serovars 4, 5 and 12 (prevalent serovars in China) vaccines against Glässer's disease. The adjuvants of mineral oil, aluminum hydroxide, Montanide GEL 01 PR, Montanide IMS 1313N VG and Montanide ISA 760 VG were used to make emulsified inactivated H. parasuis serovars 4, 5 and 12, respectively. Safety, antibody titer and protective efficacy of these vaccines were examined separately in piglets, and the feasibility of microagglutination test for detecting antibody titer of H. parasuis was confirmed for the first time. Due to easy of injection, high safety, rapidly immune responses, high concentrations of antibody, and 100% of protective efficacy for piglets, Montanide GEL 01 PR adjuvant can provide more homologous serovar protection than other domestically developed inactivated vaccines and should be used as a candidate adjuvant.
authors.affiliation: Lab of Veterinary Microbiology, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, China.
authors.forename: Qiao
authors.initials: Q
authors.lastname: Xue
authors.affiliation: Lab of Veterinary Microbiology, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, China. Electronic address: zhaozhanqin@126.com.
authors.forename: Zhanqin
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: Lab of Veterinary Microbiology, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, China.
authors.forename: Huisheng
authors.initials: H
authors.lastname: Liu
authors.affiliation: Lab of Veterinary Microbiology, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, China; Lab of Medical Engineering, College of Medical Technology and Engineering, Henan University of Science and Technology, Luoyang, China.
authors.forename: Kunpeng
authors.initials: K
authors.lastname: Chen
authors.affiliation: Lab of Medical Engineering, College of Medical Technology and Engineering, Henan University of Science and Technology, Luoyang, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Xue
authors.affiliation: Lab of Veterinary Microbiology, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, China.
authors.forename: Le
authors.initials: L
authors.lastname: Wang
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines / Q000009:adverse effects / Q000276:immunology*; D006097:Granulation Tissue / Q000473:pathology; D006192:Haemophilus Infections / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary*; D044137:Haemophilus parasuis / Q000145:classification* / Q000276:immunology; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology / Q000517:prevention & control*
chemical_list: D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines
keywords: Adjuvant; Haemophilus parasuis; Montanide GEL 01 PR; Vaccine