title: Tongue Epithelium Cells from shRNA Mediated Transgenic Goat Show High Resistance to Foot and Mouth Disease Virus.
abstract: Foot and mouth disease induced by foot and mouth disease virus (FMDV) is severe threat to cloven-hoofed domestic animals. The gene 3Dpol in FMDV genome encodes the viral RNA polymerase, a vital element for FMDV replication. In this study, a conserved 3D-7414shRNA targeting FMDV-3Dpol gene was designed and injected into pronuclear embryos to produce the transgenic goats. Sixty-one goats were produced, of which, seven goats positively integrated 3D-7414shRNA. Loss of function assay demonstrated that siRNA effectively knockdown 3Dpol gene in skin epithelium cells of transgenic goats. Subsequently, the tongue epithelium cells from transgenic and non-transgenic goats were infected with FMDV O/YS/CHA/05 strain. A significant decrease of virus titres and virus copy number was observed in cells of transgenic goats compared with that of non-transgenic goats, which indicated that 3D-7414siRNA inhibited FMDV replication by interfering FMDV-3Dpol gene. Furthermore, we found that expression of TLR7, RIG-I and TRAF6 was lower in FMDV infected cells from transgenic goats compared to that from non-transgenic goats, which might result from lower virus copy number in transgenic goats' cells. In conclusion, we successfully produced transgenic goats highly expressing 3D-7414siRNA targeting 3Dpol gene, and the tongue epithelium cells from the transgenic goats showed effective resistance to FMDV.
authors.affiliation: Department of Animal Genetics and Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China.
authors.forename: Wenting
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of Animal Genetics and Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China.
authors.forename: Kejun
authors.initials: K
authors.lastname: Wang
authors.affiliation: Department of Animal Genetics and Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China.
authors.forename: Shimeng
authors.initials: S
authors.lastname: Kang
authors.affiliation: State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China.
authors.forename: Shoulong
authors.initials: S
authors.lastname: Deng
authors.affiliation: Department of Animal Genetics and Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China.
authors.forename: Hongbing
authors.initials: H
authors.lastname: Han
authors.affiliation: Department of Animal Genetics and Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Lian
authors.affiliation: Department of Animal Genetics and Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China.
authors.forename: Zhengxing
authors.initials: Z
authors.lastname: Lian
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D060467:Disease Resistance / Q000235:genetics*; D004847:Epithelial Cells / Q000378:metabolism* / Q000821:virology*; D005536:Foot-and-Mouth Disease / Q000235:genetics* / Q000821:virology*; D029544:Foot-and-Mouth Disease Virus; D015870:Gene Expression; D020869:Gene Expression Profiling; D055785:Gene Knockdown Techniques; D006041:Goats; D034622:RNA Interference; D034741:RNA, Small Interfering; D011991:Receptors, Virus / Q000235:genetics / Q000378:metabolism; D014059:Tongue / Q000166:cytology*
chemical_list: D034741:RNA, Small Interfering; D011991:Receptors, Virus