title: The ability of multipotent mesenchymal stromal cells from the bone marrow of patients with leukemia to maintain normal hematopoietic progenitor cells.
abstract: BACKGROUND
  The development of leukemia impairs normal hematopoiesis and marrow stromal microenvironment. The aim of the investigation was to study the ability of multipotent mesenchymal stromal cells (MSCs) derived from the bone marrow of patients with leukemia to maintain normal hematopoietic progenitor cells.
  
  
  METHODS
  MSCs were obtained from the bone marrow of 14 patients with acute lymphoblastic (ALL), 25 with myeloid (AML), and 15 with chronic myeloid (CML) leukemia. As a control, MSCs from 22 healthy donors were used. The incidence of cobblestone area forming cells (CAFC 7-8 d) in the bone marrow of healthy donor cultivated on the supportive layer of patients MSCs was measured.
  
  
  RESULTS
  The ability of MSCs from AML and ALL patients at the moment of diagnosis to maintain normal CAFC was significantly decreased when compared to donors. After chemotherapy, the restoration of ALL patients' MSCs functions was slower than that of AML. CML MSCs maintained CAFC better than donors' at the moment of diagnosis and this ability increased with treatment.
  
  
  CONCLUSIONS
  The ability of patients' MSCs to maintain normal hematopoietic progenitor cells was shown to change in comparison with MSCs from healthy donors and depended on nosology. During treatment, the functional capacity of patients' MSCs had been partially restored.
authors.affiliation: National Research Center for Hematology, Moscow, Russia.
authors.forename: Tamara
authors.initials: T
authors.lastname: Sorokina
authors.affiliation: National Research Center for Hematology, Moscow, Russia.
authors.forename: Irina
authors.initials: I
authors.lastname: Shipounova
authors.affiliation: National Research Center for Hematology, Moscow, Russia.
authors.forename: Alexey
authors.initials: A
authors.lastname: Bigildeev
authors.affiliation: National Research Center for Hematology, Moscow, Russia.
authors.forename: Nataliya
authors.initials: N
authors.lastname: Petinati
authors.affiliation: National Research Center for Hematology, Moscow, Russia.
authors.forename: Nina
authors.initials: N
authors.lastname: Drize
authors.affiliation: National Research Center for Hematology, Moscow, Russia.
authors.forename: Anna
authors.initials: A
authors.lastname: Turkina
authors.affiliation: National Research Center for Hematology, Moscow, Russia.
authors.forename: Ekaterina
authors.initials: E
authors.lastname: Chelysheva
authors.affiliation: National Research Center for Hematology, Moscow, Russia.
authors.forename: Oleg
authors.initials: O
authors.lastname: Shukhov
authors.affiliation: National Research Center for Hematology, Moscow, Russia.
authors.forename: Larisa
authors.initials: L
authors.lastname: Kuzmina
authors.affiliation: National Research Center for Hematology, Moscow, Russia.
authors.forename: Elena
authors.initials: E
authors.lastname: Parovichnikova
authors.affiliation: National Research Center for Hematology, Moscow, Russia.
authors.forename: Valery
authors.initials: V
authors.lastname: Savchenko
mesh_terms: D000293:Adolescent; D000328:Adult; D001853:Bone Marrow / Q000473:pathology; D016022:Case-Control Studies; D002452:Cell Count; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002478:Cells, Cultured; D005260:Female; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D007938:Leukemia / Q000473:pathology*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008875:Middle Aged; D055815:Young Adult
keywords: acute lymphoblastic leukemia; acute myeloid leukemia; chronic myeloid leukemia; cobblestone area forming cells; multipotent mesenchymal stromal cells