title: Development of polymorphic microsatellite markers for the Killarney Fern (Vandenboschia speciosa, Hymenophyllaceae).
abstract: OBJECTIVE
  We characterize 10 microsatellite loci in the endangered fern Vandenboschia speciosa (Hymenophyllaceae), enabling studies on the genetic population structure of this Macaronesian-European species using DNA hypervariable markers.
  
  
  RESULTS
  Ten primer sets were developed and tested on 47 individuals in a total of two Iberian populations of V. speciosa. The primers amplified di- and hexanucelotide repeats. The number of alleles ranged from two to eight, and the expected heterozygosity ranged from 0.107 to 0.807 among the populations analyzed.
  
  
  CONCLUSIONS
  The 10 microsatellite markers developed will be useful in characterizing the genetic diversity of V. speciosa and understanding its population structure (including the possible structure between sporophyte and gametophyte phases) and biogeographic history, and will provide important genetic data for the conservation of this species.
authors.affiliation: Department of Botany, Faculty of Sciences, Universidad de Granada, Calle Severo Ochoa s/n, 18071 Granada, Spain.
authors.forename: M Del Carmen
authors.initials: Mdel C
authors.lastname: García-López
authors.affiliation: Department of Botany, Faculty of Sciences, Universidad de Granada, Calle Severo Ochoa s/n, 18071 Granada, Spain.
authors.forename: Samira Ben-Menni
authors.initials: SB
authors.lastname: Schuler
authors.affiliation: Department of Genetics, Faculty of Sciences, Universidad de Granada, Calle Severo Ochoa s/n, 18071 Granada, Spain.
authors.forename: Inmaculada
authors.initials: I
authors.lastname: López-Flores
authors.affiliation: Department of Botany, Faculty of Sciences, Universidad de Granada, Calle Severo Ochoa s/n, 18071 Granada, Spain.
authors.forename: Marta
authors.initials: M
authors.lastname: Nieto-Lugilde
authors.affiliation: Department of Botany, Faculty of Sciences, Universidad de Granada, Calle Severo Ochoa s/n, 18071 Granada, Spain.
authors.forename: Laura
authors.initials: L
authors.lastname: Terrón-Camero
authors.affiliation: Department of Botany, Faculty of Sciences, Universidad de Granada, Calle Severo Ochoa s/n, 18071 Granada, Spain.
authors.forename: Ismael Mazuecos
authors.initials: IM
authors.lastname: Aguilera
authors.affiliation: Department of Botany, Faculty of Sciences, Universidad de Granada, Calle Severo Ochoa s/n, 18071 Granada, Spain.
authors.forename: Víctor N
authors.initials: VN
authors.lastname: Suárez-Santiago
keywords: Hymenophyllaceae; Vandenboschia speciosa; endangered fern; microsatellite markers; population genetics