title: Increased urinary levels of podocyte glycoproteins, matrix metallopeptidases, inflammatory cytokines, and kidney injury biomarkers in women with preeclampsia.
abstract: To investigate kidney injury in preeclampsia, we analyzed 14 biomarkers in urine specimen from 4 groups of pregnant women (normotensive pregnant women and those with pregnancy complicated with chronic hypertension or mild or severe preeclampsia). These biomarkers included 1) podocyte glycoproteins nephrin and podocalyxin, 2) matrix metallopeptidase (MMP)-2 and MMP-9 and their inhibitor tissue inhibitor of metalloproteinase-2, 3) inflammatory molecules and cytokines soluble VCAM-1, TNF-α, soluble TNF receptor receptor-1, IL-6, IL-8, IL-10, and IL-18, and 4) kidney injury biomarkers neutrophil gelatinase-associated lipocalin and kidney injury molecule-1. Postpartum urine specimens (6-8 wk) from normotensive women and those with severe preeclampsia were also evaluated. We found that, first, urine levels of nephrin, MMP-2, MMP-9, and kidney injury molecule-1 were significantly higher before delivery in severe preeclampsia than normotensive groups. The increased levels were all reduced to levels similar to those of the normotensive control group in postpartum specimens from the severe preeclampsia group. Second, soluble VCAM-1, soluble TNF receptor-1, and neutrophil gelatinase-associated lipocalin levels were significantly increased in the severe preeclampsia group compared with the normotensive control group before delivery, but levels of these molecules were significantly reduced in postpartum specimens in both groups. Third, IL-6 and IL-8 levels were not different between preeclampsia and normotensive groups but significantly increased in pregnancy complicated with chronic hypertension. Finally, tissue inhibitor of metalloproteinase-2 and IL-18 levels were not different among the study groups before delivery but were significantly reduced in postpartum specimens from normotensive controls. Our results indicate that the kidney experiences an increased inflammatory response during pregnancy. Most interestingly, tubular epithelial cell injury may also occur in severe preeclampsia. These biomarkers could be used to assess podocyte or tubular injury and kidney inflammatory responses during pregnancy and to evaluate postpartum kidney injury recovery in pregnancy-complicated disorders.
authors.affiliation: Department of Obstetrics and Gynecology, Louisiana State University Health Sciences Center, Shreveport, Louisiana; and Department of Molecular and Cellular Physiology, Louisiana State University Health Sciences Center, Shreveport, Louisiana ywang1@lsuhsc.edu.
authors.forename: Yuping
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Obstetrics and Gynecology, Louisiana State University Health Sciences Center, Shreveport, Louisiana; and.
authors.forename: Yang
authors.initials: Y
authors.lastname: Gu
authors.affiliation: Department of Obstetrics and Gynecology, Louisiana State University Health Sciences Center, Shreveport, Louisiana; and.
authors.forename: Susan
authors.initials: S
authors.lastname: Loyd
authors.affiliation: Department of Obstetrics and Gynecology, Louisiana State University Health Sciences Center, Shreveport, Louisiana; and.
authors.forename: Xiuyue
authors.initials: X
authors.lastname: Jia
authors.affiliation: Department of Obstetrics and Gynecology, Louisiana State University Health Sciences Center, Shreveport, Louisiana; and.
authors.forename: Lynn J
authors.initials: LJ
authors.lastname: Groome
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000652:urine; D001794:Blood Pressure / Q000276:immunology; D016207:Cytokines / Q000276:immunology / Q000652:urine*; D005260:Female; D006023:Glycoproteins / Q000276:immunology / Q000652:urine*; D006801:Humans; D007668:Kidney / Q000276:immunology / Q000293:injuries / Q000378:metabolism; D020782:Matrix Metalloproteinases / Q000652:urine*; D050199:Podocytes / Q000276:immunology / Q000378:metabolism*; D011225:Pre-Eclampsia / Q000652:urine*; D011247:Pregnancy; D055815:Young Adult
chemical_list: D015415:Biomarkers; D016207:Cytokines; D006023:Glycoproteins; D020782:Matrix Metalloproteinases
keywords: kidney inflammatory response; podocyte glycoproteins; preeclampsia; pregnancy; tubular injury