title: Good use of fruit wastes: eco-friendly synthesis of silver nanoparticles, characterization, BSA protein binding studies.
abstract: A simple and eco-friendly methodology for the green synthesis of silver nanoparticles (AgNPs) using a mango seed extract was evaluated. The AgNPs were characterized by ultraviolet-visible spectrophotometry, Fourier transform infrared spectroscopy, transmission electron microscopy, energy dispersive X-ray spectroscopy, and X-ray diffraction. The interaction between the green synthesized AgNPs and bovine serum albumin (BSA) in an aqueous solution at physiological pH was examined by fluorescence spectroscopy. The results confirmed that the AgNPs quenched the fluorophore of BSA by forming a ground state complex in aqueous solution. This fluorescence quenching data were also used to determine the binding sites and binding constants at different temperatures. The calculated thermodynamic parameters (ΔG°, ΔH° and ΔS°) suggest that the binding process occurs spontaneously through the involvement of electrostatic interactions. The synchronous fluorescence spectra showed a blue shift, indicating increasing hydrophobicity. Copyright © 2015 John Wiley & Sons, Ltd.
authors.affiliation: Department of Chemical Engineering, Yeungnam University, Gyeongsan-Si, Korea.
authors.forename: T V M
authors.initials: TV
authors.lastname: Sreekanth
authors.affiliation: Department of Biotechnology and Bio-Engineering, College of Engineering Sciences, Chonnam National University, Gwangju, 500-757, Korea.
authors.forename: Sambandam
authors.initials: S
authors.lastname: Ravikumar
authors.affiliation: Department of Chemical Engineering, Yeungnam University, Gyeongsan-Si, Korea.
authors.forename: Yong Rok
authors.initials: YR
authors.lastname: Lee
mesh_terms: D001665:Binding Sites; D055772:Green Chemistry Technology; D031022:Mangifera / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D010936:Plant Extracts / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism*; D012834:Silver / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D013816:Thermodynamics; D014961:X-Ray Diffraction
chemical_list: D010936:Plant Extracts; D012710:Serum Albumin, Bovine; D012834:Silver
keywords: green synthesis; mango seed; protein binding parameters; silver nanoparticles; thermodynamic parameters