title: Protective Effects of Diallyl Sulfide on Ovalbumin-Induced Pulmonary Inflammation of Allergic Asthma Mice by MicroRNA-144, -34a, and -34b/c-Modulated Nrf2 Activation.
abstract: Allergic airway disorder is characterized by an increase in the level of reactive oxygen species (ROS). The induction of inflammation and hyperresponsiveness by an allergen was ameliorated by antioxidants in vivo. This study investigated the protective effects and underlying mechanism of diallyl sulfide (DAS) on ovalbumin (OVA)-induced allergic asthma of BALB/c mice. The animals were intraperitoneally sensitized by inhaling OVA to induce chronic airway inflammation. By administering DAS, a decrease of the infiltrated inflammatory cell counts and the levels of IL-4 and IL-10 in bronchoalveolar lavage fluid as well as the OVA-specific immunoglobulin E levels in sera were observed. DAS also effectively inhibited OVA-induced inflammatory cell infiltration and mucus hypersecretion in lung tissue. Several OVA-induced inflammatory factors (ROS, 8-hydroxy-2'-deoxyguanosine, 8-iso-prostaglandin F2α, and NF-κB) were inhibited by DAS. In addition, DAS increased OVA inhalation-reduced levels of Nrf2 activation by regulating microRNA-144, -34a and -34b/c. Together, the pathogenesis of OVA-induced asthma is highly associated with oxidative stress, and DAS may be an effective supplement to alleviate this disease.
authors.affiliation: Department of Food Science and Biotechnology, National Chung Hsing University , 250 Kuokuang Road, Taichung 40227, Taiwan.
authors.forename: Cheng-Ying
authors.initials: CY
authors.lastname: Ho
authors.affiliation: Department of Food Science and Biotechnology, National Chung Hsing University , 250 Kuokuang Road, Taichung 40227, Taiwan.|School of Nutrition and Health Sciences, Taipei Medical University , 250 Wu-Hsing Street, Taipei 11031, Taiwan.
authors.forename: Chi-Cheng
authors.initials: CC
authors.lastname: Lu
authors.affiliation: Graduate Institute of Applied Living Science, Tainan University of Technology , 529 Zhongzheng Road, Yongkang District, Tainan City 71002, Taiwan.
authors.forename: Chia-Jui
authors.initials: CJ
authors.lastname: Weng
authors.affiliation: Department of Food Science and Biotechnology, National Chung Hsing University , 250 Kuokuang Road, Taichung 40227, Taiwan.|Agricultural Biotechnology Center, National Chung Hsing University , 250 Kuokuang Road, Taichung 40227, Taiwan.
authors.forename: Gow-Chin
authors.initials: GC
authors.lastname: Yen
mesh_terms: D000818:Animals; D018927:Anti-Asthmatic Agents / Q000008:administration & dosage*; D001249:Asthma / Q000139:chemically induced / Q000235:genetics / Q000276:immunology / Q000517:prevention & control*; D005260:Female; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics / Q000276:immunology; D015847:Interleukin-4 / Q000235:genetics / Q000276:immunology; D008168:Lung / Q000187:drug effects / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D035683:MicroRNAs / Q000235:genetics / Q000276:immunology*; D051267:NF-E2-Related Factor 2 / Q000235:genetics* / Q000276:immunology; D010047:Ovalbumin / Q000009:adverse effects; D013440:Sulfides / Q000008:administration & dosage*
chemical_list: D018927:Anti-Asthmatic Agents; C551064:MIRN144 microRNA, mouse; C551848:MIRN34a microRNA, mouse; D035683:MicroRNAs; D051267:NF-E2-Related Factor 2; C495636:Nfe2l2 protein, mouse; D013440:Sulfides; D016753:Interleukin-10; D015847:Interleukin-4; D010047:Ovalbumin
keywords: Nrf2; asthma; diallyl sulfide; inflammation; ovalbumin