title: Copper-64 Dichloride as Theranostic Agent for Glioblastoma Multiforme: A Preclinical Study.
abstract: Glioblastoma multiforme (GBM) is the most common primary malignant brain tumor in adults with a median survival time less than one year. To date, there are only a limited number of effective agents available for GBM therapy and this does not seem to add much survival advantage over the conventional approach based on surgery and radiotherapy. Therefore, the development of novel therapeutic approaches to GBM is essential and those based on radionuclide therapy could be of significant clinical impact. Experimental evidence has clearly demonstrated that cancer cells have a particularly high fractional content of copper inside the nucleus compared to normal cells. This behavior can be conveniently exploited both for diagnosis and for delivering therapeutic payloads (theranostic) of the radionuclide copper-64 into the nucleus of cancerous cells by intravenous administration of its simplest chemical form as dichloride salt [(64)Cu]CuCl2. To evaluate the potential theranostic role of [(64)Cu]CuCl2 in GBM, the present work reports results from a preclinical study carried out in a xenografted GBM tumor mouse model. Biodistribution data of this new agent were collected using a small-animal PET tomograph. Subsequently, groups of tumor implanted nude mice were treated with [(64)Cu]CuCl2 to simulate single- and multiple-dose therapy protocols, and results were analyzed to estimate therapeutic efficacy.
authors.affiliation: Nuclear Medicine, University "Aldo Moro", Piazza Giulio Cesare 11, 70124 Bari, Italy.
authors.forename: Cristina
authors.initials: C
authors.lastname: Ferrari
authors.affiliation: Nuclear Medicine, University "Aldo Moro", Piazza Giulio Cesare 11, 70124 Bari, Italy.
authors.forename: Artor Niccoli
authors.initials: AN
authors.lastname: Asabella
authors.affiliation: Nuclear Medicine, Hospital "Spirito Santo", Pescara, Italy.
authors.forename: Carlo
authors.initials: C
authors.lastname: Villano
authors.affiliation: Advanced Center Oncology Macerata (ACOM) S.p.A., Montecosaro, Italy.
authors.forename: Beatrice
authors.initials: B
authors.lastname: Giacobbi
authors.affiliation: Advanced Center Oncology Macerata (ACOM) S.p.A., Montecosaro, Italy.
authors.forename: Daniela
authors.initials: D
authors.lastname: Coccetti
authors.affiliation: Advanced Center Oncology Macerata (ACOM) S.p.A., Montecosaro, Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Panichelli
authors.affiliation: Nuclear Medicine, University "Aldo Moro", Piazza Giulio Cesare 11, 70124 Bari, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Rubini
mesh_terms: D000818:Animals; D001921:Brain / Q000000981:diagnostic imaging / Q000473:pathology* / Q000528:radiation effects; D045744:Cell Line, Tumor; D003131:Combined Modality Therapy; D003301:Copper Radioisotopes / Q000627:therapeutic use*; D005909:Glioblastoma / Q000000981:diagnostic imaging / Q000473:pathology / Q000532:radiotherapy*; D006801:Humans; D051379:Mice; D000068936:Theranostic Nanomedicine; D014018:Tissue Distribution; D014057:Tomography, X-Ray Computed; D023041:Xenograft Model Antitumor Assays
chemical_list: D003301:Copper Radioisotopes