title: Multivariate calibration-assisted high-performance liquid chromatography with dual UV and fluorimetric detection for the analysis of natural and synthetic sex hormones in environmental waters and sediments.
abstract: A green method is reported based on non-sophisticated instrumental for the quantification of seven natural and synthetic estrogens, three progestagens and one androgen in the presence of real interferences. The method takes advantage of: (1) chromatography, allowing total or partial resolution of a large number of compounds, (2) dual detection, permitting selection of the most appropriate signal for each analyte and, (3) second-order calibration, enabling mathematical resolution of incompletely resolved chromatographic bands and analyte determination in the presence of interferents. Consumption of organic solvents for cleaning, extraction and separation are markedly decreased because of the coupling with MCR-ALS (multivariate curve resolution/alternating least-squares) which allows the successful resolution in the presence of other co-eluting matrix constituents. Rigorous IUPAC detection limits were obtained: 6-24 ng L(-1) in water, and 0.1-0.9 ng g(-1) in sediments. Relative prediction errors were 2-10% (water) and 1-8% (sediments).
authors.affiliation: Instituto de Química Rosario (CONICET-UNR), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 531, 2000 Rosario, Argentina.
authors.forename: Rocío L
authors.initials: RL
authors.lastname: Pérez
authors.affiliation: Instituto de Química Rosario (CONICET-UNR), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 531, 2000 Rosario, Argentina. Electronic address: escandar@iquir-conicet.gov.ar.
authors.forename: Graciela M
authors.initials: GM
authors.lastname: Escandar
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D005470:Fluorometry / Q000379:methods*; D019015:Geologic Sediments / Q000032:analysis*; D012739:Gonadal Steroid Hormones / Q000032:analysis* / Q000138:chemical synthesis; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D012739:Gonadal Steroid Hormones; D014874:Water Pollutants, Chemical
keywords: Dual UV-fluorescence detection; Endocrine disruptors; Environmental samples; High-performance-liquid chromatography; Multivariate curve resolution