title: Expression of STK39 in peripheral blood of hypertension patients and the relationship between its genetic polymorphism and blood pressure.
abstract: This study investigated the STK39 expression in peripheral blood of hypertension patients and the relation between its genetic polymorphism and blood pressure. The observation group comprised of 42 primary hypertension patients admitted to our hospital, and the control group comprised of 30 healthy individuals who underwent physical examination in our hospital during the same period. Fasting venous blood was collected from both groups in the morning to determine the STK39 mRNA and protein levels in peripheral blood using quantitative real-time PCR and western blot. STK39 gene SNP (rs6433027) was sequenced using PCR and its genetic variation was analyzed. The relationship between STK39 protein level, genetic variation, and diastolic and systolic blood pressure was also analyzed. The observation group showed increased STK39 mRNA and protein levels in peripheral blood compared to the control group, and the difference was statistically significant (P < 0.05), suggesting C/T mutation in STK39 gene SNP (rs6433027). Correlation analysis showed positive association between STK39 protein level and diastolic and systolic blood pressure (P < 0.05), indicating a positive association between C/T genetic mutation and diastolic and systolic blood pressure (P < 0.05). In conclusion, STK39 mRNA and protein express abnormally in primary hypertension patients with genetic variation, which is related to the blood pressure.
authors.affiliation: Department of Cardiology II, The Third Affiliated Hospital of Xinxiang Medical University, Xingxiang, China.
authors.forename: B
authors.initials: B
authors.lastname: Li
authors.affiliation: Department of Cardiology I, The Third Affiliated Hospital of Xinxiang Medical University, Xingxiang, China.
authors.forename: M
authors.initials: M
authors.lastname: Yang
authors.affiliation: Department of Cardiology II, The Third Affiliated Hospital of Xinxiang Medical University, Xingxiang, China.
authors.forename: J W
authors.initials: JW
authors.lastname: Liu
mesh_terms: D000368:Aged; D001483:Base Sequence; D015415:Biomarkers; D001794:Blood Pressure / Q000235:genetics*; D016022:Case-Control Studies; D000075222:Essential Hypertension; D005260:Female; D015870:Gene Expression; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006801:Humans; D006973:Hypertension / Q000097:blood / Q000235:genetics* / Q000503:physiopathology*; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D017346:Protein Serine-Threonine Kinases / Q000097:blood / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D015415:Biomarkers; D012333:RNA, Messenger; D017346:Protein Serine-Threonine Kinases; C486409:STK39 protein, human