title: [B cells from streptozotocin-induced mouse diabetic model inhibit insulin secretion of MIN-6 cells and promote production of insulin autoantibody].
abstract: OBJECTIVE
  To explore the role of B cells in the progression of mouse diabetes induced by streptozotocin (STZ).
  
  
  METHODS
  The ICR mouse diabetic model was induced by low-dose STZ injection. Radioimmunoassay was performed to examine the serum levels of insulin, and ELISA was conducted to detect insulin autoantibody (IAA). B cells from STZ-treated mice and control group were sorted by CD19 magnetic activated cell sorting (MACS), and cell purity was verified by flow cytometry. The PBS-treated MIN-6 cells were used as blank control group (group A); the isolated normal mouse B cells were incubated with MIN-6 cells as group B; B cells isolated from normal mice were pre-stimulated with 20 μg/mL lipopolysaccharides (LPS), then were incubated with MIN-6 cells, as group C; and B cells isolated from STZ-treated mice were incubated with MIN-6 cells, as group D. The mRNA and protein levels of insulin were detected by quantitative real-time PCR (qRT-PCR) and radioimmunoassay, respectively. The levels of IAA and transforming growth factor β(TGF-β) were detected by ELISA.
  
  
  RESULTS
  Compared with group A, STZ-treated mice showed reduced body mass and serum insulin, but increased blood glucose and IAA levels. Flow cytometry indicated that the proportion of CD19(+) B cells were 98%. Compared with group A or B, group C or D presented with decreased mRNA and protein levels of insulin in MIN-6 cells, simultaneously, increased expressions of IAA and TGF-β.
  
  
  CONCLUSIONS
  B cells isolated from STZ-treated mice promotes the production of IAA and inhibits the secretion of insulin in MIN-6 cells.
authors.affiliation: Department of Immunology, College of Basic Medicine, Anhui Medical University, Hefei 230032; Department of Clinical Laboratory, Second People' s Hospital of Anhui Province, Hefei 230011, China.
authors.forename: Man
authors.initials: M
authors.lastname: Qin
authors.affiliation: Department of Clinical Laboratory, Second People' s Hospital of Anhui Province, Hefei 230011, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Xu
authors.affiliation: Department of Clinical Laboratory, Second People' s Hospital of Anhui Province, Hefei 230011, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.affiliation: Department of Clinical Laboratory, Second People' s Hospital of Anhui Province, Hefei 230011, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Tang
authors.affiliation: Department of Clinical Laboratory, Second People' s Hospital of Anhui Province, Hefei 230011, China.
authors.forename: Jingru
authors.initials: J
authors.lastname: Sun
authors.affiliation: Department of Immunology, College of Basic Medicine, Anhui Medical University, Hefei 230032, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wei
authors.affiliation: Department of Immunology, College of Basic Medicine, Anhui Medical University, Hefei 230032, China. *Corresponding author, E-mail: huangbaojun516@163.com.
authors.forename: Baojun
authors.initials: B
authors.lastname: Huang
mesh_terms: D000818:Animals; D001323:Autoantibodies / Q000378:metabolism*; D001402:B-Lymphocytes / Q000378:metabolism*; D002460:Cell Line; D049109:Cell Proliferation; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000139:chemically induced / Q000503:physiopathology; D004195:Disease Models, Animal; D007328:Insulin / Q000097:blood*; D007330:Insulin Antibodies / Q000378:metabolism*; D050417:Insulin-Secreting Cells / Q000378:metabolism*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D013311:Streptozocin / Q000009:adverse effects; D016212:Transforming Growth Factor beta / Q000378:metabolism
chemical_list: D001323:Autoantibodies; D007328:Insulin; D007330:Insulin Antibodies; D016212:Transforming Growth Factor beta; D013311:Streptozocin