title: Role of Msi1 and PYGO2 in esophageal squamous cell carcinoma depth of invasion.
abstract: Deregulation of developmental signaling pathways such as Wnt/b-catenin and NOTCH are commonly observed in different cancers. A normal wnt pathway is essential for development and tissue homeostasis to preserve a normal balance between the differentiation and proliferation. PYGO2 is the main transcription factor of wnt pathway, while Msi1 is one of the wnt inhibitors. In this study we assessed the correlation between Msi1 and PYGO2 mRNA expression using Real time polymerase chain reaction in 48 esophageal squamous cell carcinoma (ESCC) patients. Although, there was not any significant correlation between the levels of Msi1 and PYGO2 mRNA expression, we observed a significant correlation between the Msi1 and PYGO2 overexpressed cases and depth of tumor invasion (p = 0.05). In conclusion, despite the role of these markers in tumor depth of invasion there is not any feedback between Msi1 and PYGO2 gene expression in ESCC.
authors.affiliation: Division of Human Genetics, Immunology Research Center, Avicenna Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Meysam
authors.initials: M
authors.lastname: Moghbeli
authors.affiliation: Cardiothoracic Surgery and Transplant Research Center, Imam Reza Hospital, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Sadrizadeh
authors.affiliation: Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran.
authors.forename: Mohammad Mahdi
authors.initials: MM
authors.lastname: Forghanifard
authors.affiliation: Department of Gastroenterology, Imam Reza Hospital, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Hooman Mosannen
authors.initials: HM
authors.lastname: Mozaffari
authors.affiliation: Department of Anesthesiology and Critical Care, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Ebrahim
authors.initials: E
authors.lastname: Golmakani
authors.affiliation: Division of Human Genetics, Immunology Research Center, Avicenna Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran. abbaszadeganmr@mums.ac.ir.|Medical Genetics Research Center, Medical School, Mashhad University of Medical Sciences, Mashhad, Iran. abbaszadeganmr@mums.ac.ir.
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Abbaszadegan
keywords: Cancer stem cell; Expressional analysis; Metastasis; NOTCH pathway; Suppressor; Translation; Tumor; Wnt pathway