title: Simultaneous Determination of Menthol and Eucalyptol by the Densitometric HPTLC Method in Some External Analgesic Formulations.
abstract: Several analgesic pharmaceutical formulations dispensed for external use contain menthol and eucalyptol. Simultaneous determination of the two monoterpenes was achieved utilizing high-performance thin layer chromatography (HPTLC) densitometric analysis. The stationary phase composed of silica gel 60 F254 (E-Merck, Germany) glass-backed plates coated with 0.2 mm layers. The used mobile phase hexane : ethyl acetate 8 : 2 (%, v/v) resulted in sharp, symmetrical and well-resolved peak at an Rf value of 0.34 ± 0.04 and 0.56 ± 0.04 for menthol and eucalyptol, respectively. Linearly range for menthol was 100-800 ng/spot (r(2) = 0.9979), whereas that for eucalyptol was 52.25-1107 ng/spot (r(2) = 0.9937). The developed method was validated following the International Conference on Harmonization guidelines. The method was found to be simple, accurate, time saving and enable analyses of many samples simultaneously.
authors.affiliation: Department of Pharmacognosy, College of Pharmacy, Salman Bin Abdulaziz University, PO Box 173, Al-Kharj 11942, Saudi Arabia prawez_pharma@yahoo.com.
authors.forename: Prawez
authors.initials: P
authors.lastname: Alam
authors.affiliation: Department of Pharmacognosy, College of Pharmacy, King Saud University, PO Box 2457, Riyadh 11451, Saudi Arabia.
authors.forename: Saleh I
authors.initials: SI
authors.lastname: Alqasoumi
authors.affiliation: Department of Pharmacognosy, College of Pharmacy, Salman Bin Abdulaziz University, PO Box 173, Al-Kharj 11942, Saudi Arabia Department of Pharmacognosy, College of Pharmacy, Alexandria University, Alexandria 21215, Egypt.
authors.forename: Maged S
authors.initials: MS
authors.lastname: Abdel-Kader
mesh_terms: D000700:Analgesics / Q000737:chemistry*; D002855:Chromatography, Thin Layer / Q000379:methods*; D003511:Cyclohexanols / Q000032:analysis*; D000077591:Eucalyptol; D057230:Limit of Detection; D008610:Menthol / Q000032:analysis*; D039821:Monoterpenes / Q000032:analysis*; D015203:Reproducibility of Results
chemical_list: D000700:Analgesics; D003511:Cyclohexanols; D039821:Monoterpenes; D008610:Menthol; D000077591:Eucalyptol