title: A versatile Multisite Gateway-compatible promoter and transgenic line collection for cell type-specific functional genomics in Arabidopsis.
abstract: Multicellular organisms are composed of many cell types that acquire their specific fate through a precisely controlled pattern of gene expression in time and space dictated in part by cell type-specific promoter activity. Understanding the contribution of highly specialized cell types in the development of a whole organism requires the ability to isolate or analyze different cell types separately. We have characterized and validated a large collection of root cell type-specific promoters and have generated cell type-specific marker lines. These benchmarked promoters can be readily used to evaluate cell type-specific complementation of mutant phenotypes, or to knockdown gene expression using targeted expression of artificial miRNA. We also generated vectors and characterized transgenic lines for cell type-specific induction of gene expression and cell type-specific isolation of nuclei for RNA and chromatin profiling. Vectors and seeds from transgenic Arabidopsis plants will be freely available, and will promote rapid progress in cell type-specific functional genomics. We demonstrate the power of this promoter set for analysis of complex biological processes by investigating the contribution of root cell types in the IRT1-dependent root iron uptake. Our findings revealed the complex spatial expression pattern of IRT1 in both root epidermis and phloem companion cells and the requirement for IRT1 to be expressed in both cell types for proper iron homeostasis.
authors.affiliation: Laboratoire de Reproduction et Développement des Plantes, UMR 5667 CNRS/INRA/ENS-Lyon/Université de Lyon, 46 allée d'Italie, 69364 Lyon Cedex 07, France.
authors.forename: Maria Del Mar
authors.initials: MDM
authors.lastname: Marquès-Bueno
authors.affiliation: Institut de Biologie de l'Ecole Normale Supérieure, UMR 8197 CNRS/INSERM, Paris 75005, France.
authors.forename: Ana Karina
authors.initials: AK
authors.lastname: Morao
authors.affiliation: Institute for Integrative Biology of the Cell, UMR 9198 CNRS/CEA/University Paris-Sud, Avenue de la Terrasse, 91190 Gif-sur-Yvette, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Cayrel
authors.affiliation: Laboratoire de Reproduction et Développement des Plantes, UMR 5667 CNRS/INRA/ENS-Lyon/Université de Lyon, 46 allée d'Italie, 69364 Lyon Cedex 07, France.
authors.forename: Matthieu Pierre
authors.initials: MP
authors.lastname: Platre
authors.affiliation: University of Lausanne, Department of Plant Molecular Biology, UNIL-Sorge, 1015 Lausanne, Switzerland.
authors.forename: Marie
authors.initials: M
authors.lastname: Barberon
authors.affiliation: Institut de Biologie de l'Ecole Normale Supérieure, UMR 8197 CNRS/INSERM, Paris 75005, France.
authors.forename: Erwann
authors.initials: E
authors.lastname: Caillieux
authors.affiliation: Institut de Biologie de l'Ecole Normale Supérieure, UMR 8197 CNRS/INSERM, Paris 75005, France.
authors.forename: Vincent
authors.initials: V
authors.lastname: Colot
authors.affiliation: Laboratoire de Reproduction et Développement des Plantes, UMR 5667 CNRS/INRA/ENS-Lyon/Université de Lyon, 46 allée d'Italie, 69364 Lyon Cedex 07, France.
authors.forename: Yvon
authors.initials: Y
authors.lastname: Jaillais
authors.affiliation: Institut de Biologie de l'Ecole Normale Supérieure, UMR 8197 CNRS/INSERM, Paris 75005, France.
authors.forename: François
authors.initials: F
authors.lastname: Roudier
authors.affiliation: Institute for Integrative Biology of the Cell, UMR 9198 CNRS/CEA/University Paris-Sud, Avenue de la Terrasse, 91190 Gif-sur-Yvette, France.
authors.forename: Grégory
authors.initials: G
authors.lastname: Vert
mesh_terms: D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D023281:Genomics / Q000379:methods*; D030821:Plants, Genetically Modified / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*
chemical_list: D029681:Arabidopsis Proteins
keywords: BREAK lines; INTACT; LINE UP lines; RED TIDE lines; SAND lines