title: [Se-ZnCu-65P enhances mouse peritoneal macrophage phagocytosis and inhibits secretion of NO and H2O2 in vitro].
abstract: OBJECTIVE
  To investigate the effect of selenopeptide on phagocytosis, NO and H2O2 secretion of mouse peritoneal macrophages.
  
  
  METHODS
  Mouse peritoneal macrophages induced by lipopolysaccharide (LPS) were cultured for 24 hours by various concentrations of Se-ZnCu-65P, which is a selenopeptide with double antioxidant activity of superoxide dismutase (SOD) and glutathione peroxidase (GPx). Then, the relative cell activity was determined by methyl thiazolyl tetrazolium (MTT) assays, the phagocytic ability of macrophages was evaluated by neutral red uptake assay, nitric oxide (NO) content was examined by nitrate reductase method, and hydrogen peroxide (H2O2) content was detected by molybdate colorimetric method.
  
  
  RESULTS
  When Se-ZnCu-65P acted on macrophages alone, the relative cell viability was enhanced, the phagocytic ability was not affected, H2O2 content was reduced, and NO level was almost unchanged. However, the phagocytic ability of macrophages induced by LPS was raised, meanwhile, secretion of NO and H2O2 was promoted. When Se-ZnCu-65P acted on macrophages induced by LPS, the phagocytic ability were further enhanced, and secretion of NO and H2O2 was inhibited significantly.
  
  
  CONCLUSIONS
  Se-ZnCu-65P can effectively improve the relative cell activity of mouse peritoneal macrophages induced by LPS, enhance the phagocytic ability of macrophages, and reduce the secretion levels of NO and H2O2.
authors.affiliation: Department of Biochemistry and Molecular Biology, Jilin Medical University, Jilin 132013, China. *Corresponding author, E-mail: wangcheng1040@163.com.
authors.forename: Cheng
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Biochemistry and Molecular Biology, Jilin Medical University, Jilin 132013, China.
authors.forename: Chunhong
authors.initials: C
authors.lastname: Sui
authors.affiliation: Ministry of Education Key Laboratory of Molecular Enzymology and Engineering, Jilin University, Changchun 130012, China.
authors.forename: Ganglin
authors.initials: G
authors.lastname: Yan
authors.affiliation: Department of Biochemistry and Molecular Biology, Jilin Medical University, Jilin 132013, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Biochemistry and Molecular Biology, Jilin Medical University, Jilin 132013, China.
authors.forename: Wengan
authors.initials: W
authors.lastname: Pan
mesh_terms: D000818:Animals; D005260:Female; D005979:Glutathione Peroxidase / Q000235:genetics / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000378:metabolism*; D017737:Macrophages, Peritoneal / Q000201:enzymology* / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009569:Nitric Oxide / Q000378:metabolism*; D010587:Phagocytosis; D012643:Selenium / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism*
chemical_list: D009569:Nitric Oxide; D006861:Hydrogen Peroxide; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D012643:Selenium