title: Inspired by Nature: The Use of Plant-derived Substrate/Enzyme Combinations to Generate Antimicrobial Activity in situ.
abstract: The last decade has witnessed a renewed interest in antimicrobial agents. Plants have received particular attention and frequently rely on the spontaneous enzymatic conversion of an inactive precursor to an active agent. Such two-component substrate/enzyme defence systems can be reconstituted ex vivo. Here, the alliin/alliinase system from garlic seems to be rather effective against Saccharomyces cerevisiae, whilst the glucosinolate/myrosinase system from mustard appears to be more active against certain bacteria. Studies with myrosinase also confirm that enzyme and substrate can be added sequentially. Ultimately, such binary systems hold considerable promise and may be employed in a medical or agricultural context.
authors.forename: Ethiene Castellucci
authors.initials: EC
authors.lastname: Estevam
authors.forename: Sharoon
authors.initials: S
authors.lastname: Griffin
authors.forename: Muhammad Jawad
authors.initials: MJ
authors.lastname: Nasim
authors.forename: Dariusz
authors.initials: D
authors.lastname: Zieliński
authors.forename: Justyna
authors.initials: J
authors.lastname: Aszyk
authors.forename: Magdalena
authors.initials: M
authors.lastname: Osowicka
authors.forename: Natalia
authors.initials: N
authors.lastname: Dawidowska
authors.forename: Rinaldi
authors.initials: R
authors.lastname: Idroes
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Bartoszek
authors.forename: Claus
authors.initials: C
authors.lastname: Jacob
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology; D000969:Antinematodal Agents / Q000737:chemistry / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D013437:Carbon-Sulfur Lyases / Q000737:chemistry / Q000378:metabolism*; D003545:Cysteine / Q000031:analogs & derivatives* / Q000737:chemistry; D005961:Glucosinolates / Q000737:chemistry*; D006026:Glycoside Hydrolases / Q000737:chemistry / Q000378:metabolism*; D009348:Nematoda / Q000187:drug effects; D012441:Saccharomyces cerevisiae / Q000187:drug effects
chemical_list: D000890:Anti-Infective Agents; D000969:Antinematodal Agents; D005961:Glucosinolates; C006453:alliin; D006026:Glycoside Hydrolases; C022739:thioglucosidase; D013437:Carbon-Sulfur Lyases; C019243:alliin lyase; D003545:Cysteine