title: Adult human dental pulp stem cells promote blood-brain barrier permeability through vascular endothelial growth factor-a expression.
abstract: Stem cell therapy is a promising new treatment option for stroke. Intravascular administration of stem cells is a valid approach as stem cells have been shown to transmigrate the blood-brain barrier. The mechanism that causes this effect has not yet been elucidated. We hypothesized that stem cells would mediate localized discontinuities in the blood-brain barrier, which would allow passage into the brain parenchyma. Here, we demonstrate that adult human dental pulp stem cells express a soluble factor that increases permeability across an in vitro model of the blood-brain barrier. This effect was shown to be the result of vascular endothelial growth factor-a. The effect could be amplified by exposing dental pulp stem cell to stromal-derived factor 1, which stimulates vascular endothelial growth factor-a expression. These findings support the use of dental pulp stem cell in therapy for stroke.
authors.affiliation: Stroke Research Programme, School of Medicine, University of Adelaide, Adelaide, Australia Centre for Stem Cell Research, Robinson Institute, Adelaide, Australia.
authors.forename: Joshua N
authors.initials: JN
authors.lastname: Winderlich
authors.affiliation: Stroke Research Programme, School of Medicine, University of Adelaide, Adelaide, Australia Centre for Stem Cell Research, Robinson Institute, Adelaide, Australia.
authors.forename: Karlea L
authors.initials: KL
authors.lastname: Kremer
authors.affiliation: Stroke Research Programme, School of Medicine, University of Adelaide, Adelaide, Australia Centre for Stem Cell Research, Robinson Institute, Adelaide, Australia Department of Neurology, Queen Elizabeth Hospital, Woodville, Australia simon.koblar@adelaide.edu.au.
authors.forename: Simon A
authors.initials: SA
authors.lastname: Koblar
mesh_terms: D000328:Adult; D001812:Blood-Brain Barrier / Q000378:metabolism*; D064987:Cell- and Tissue-Based Therapy; D002478:Cells, Cultured; D054377:Chemokine CXCL12; D003782:Dental Pulp / Q000166:cytology*; D006801:Humans; D010539:Permeability; D033581:Stem Cell Transplantation / Q000379:methods*; D013234:Stem Cells / Q000378:metabolism / Q000502:physiology*; D020521:Stroke / Q000628:therapy*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism; D055815:Young Adult
chemical_list: D054377:Chemokine CXCL12; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A
keywords: Dental pulp stem cells; blood–brain barrier; in vitro; stromal-derived factor 1; vascular endothelial growth factor