title: [The effect of paraquat on voltage-dependent anion channel and caspase-3, 8, 9 in the mitochondria of rat lung].
abstract: OBJECTIVE
  To investigate the effects of different concentrations of paraquat (PQ) poisoning on the expression of voltage-dependent anion channel (VDAC) and caspase family in the mitochondria of rat lung tissue, and to explore possible mechanisms of acute lung injury induced by acute PQ poisoning.
  
  
  METHODS
  Two hundred healthy adult Wister rats with equal numbers of male and female ones were randomly and equally divided into control group and poisoned group. The control group received one-time gastric lavage with 1 ml of normal saline, and the poisoned group with PQ (50 mg/kg) diluted in 1 ml of normal saline. Twenty rats were collected at 1, 24, 72, 120, and 168 h after lavage with normal saline or PQ and dissected after anesthesia. Mitochondria were separated from rat lung tissue, and the content of VDAC and caspase-3, -8, and -9 were determined.
  
  
  RESULTS
  The expression of VDAC and caspase-3, -8, and -9 in the poisoned rats were significantly higher than that in the control group (P < 0.001). At 1, 24, 72, 120, and 168 h after exposure, acute diffuse damages were found in alveolar capillary endothelial cells, alveolar epithelial cells, and pulmonary interstitial cells. Inflammatory cell infiltration in the pulmonary interstitium, alveolar structural disorder, and substantially increased fibroblasts were also found in rat lung tissue.
  
  
  CONCLUSIONS
  PQ poisoning can up-regulate the expression of VDAC and caspase-3, -8, and -9 in mitochondria of rat lung tissue to induce acute lung injury.
authors.affiliation: Hangzhou Normal University Affiliated Hospital, Hangzhou 310015, China.
authors.forename: Dengpan
authors.initials: D
authors.lastname: Lai
authors.affiliation: Hangzhou Normal University Affiliated Hospital, Hangzhou 310015, China.
authors.forename: Jinming
authors.initials: J
authors.lastname: Xia
authors.affiliation: Hangzhou Normal University Affiliated Hospital, Hangzhou 310015, China.
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Wang
authors.affiliation: Hangzhou Normal University Affiliated Hospital, Hangzhou 310015, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Wei
authors.affiliation: Hangzhou Normal University Affiliated Hospital, Hangzhou 310015, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Qian
authors.affiliation: Hangzhou Normal University Affiliated Hospital, Hangzhou 310015, China.
authors.forename: Qiuying
authors.initials: Q
authors.lastname: Lou
authors.affiliation: Hangzhou Normal University Affiliated Hospital, Hangzhou 310015, China.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Ren
authors.affiliation: Hangzhou Normal University Affiliated Hospital, Hangzhou 310015, China.
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Huang
mesh_terms: D055371:Acute Lung Injury / Q000139:chemically induced / Q000473:pathology*; D000818:Animals; D053148:Caspase 3 / Q000378:metabolism; D053181:Caspase 8 / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D020169:Caspases / Q000378:metabolism*; D005260:Female; D008168:Lung / Q000187:drug effects / Q000473:pathology; D008297:Male; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism; D010269:Paraquat / Q000506:poisoning*; D051381:Rats; D017207:Rats, Sprague-Dawley; D050994:Voltage-Dependent Anion Channels / Q000378:metabolism*
chemical_list: D050994:Voltage-Dependent Anion Channels; C505410:Casp3 protein, rat; C505528:Casp8 protein, rat; C506435:Casp9 protein, rat; D053148:Caspase 3; D053181:Caspase 8; D053453:Caspase 9; D020169:Caspases; D010269:Paraquat