title: Design of a predicted MHC restricted short peptide immunodiagnostic and vaccine candidate for Fowl adenovirus C in chicken infection.
abstract: Fowl adenoviruses (FAdVs) are the ethiologic agents of multiple pathologies in chicken. There are five different species of FAdVs grouped as FAdV-A, FAdV-B, FAdV-C, FAdV-D, and FAdV-E. It is of interest to develop immunodiagnostics and vaccine candidate for Peruvian FAdV-C in chicken infection using MHC restricted short peptide candidates. We sequenced the complete genome of one FAdV strain isolated from a chicken of a local farm. A total of 44 protein coding genes were identified in each genome. We sequenced twelve Cobb chicken MHC alleles from animals of different farms in the central coast of Peru, and subsequently determined three optimal human MHC-I and four optimal human MHC-II substitute alleles for MHC-peptide prediction. The potential MHC restricted short peptide epitope-like candidates were predicted using human specific (with determined suitable chicken substitutes) NetMHC MHC-peptide prediction model with web server features from all the FAdV genomes available. FAdV specific peptides with calculated binding values to known substituted chicken MHC-I and MHC-II were further filtered for diagnostics and potential vaccine epitopes. Promiscuity to the 3/4 optimal human MHC-I/II alleles and conservation among the available FAdV genomes was considered in this analysis. The localization on the surface of the protein was considered for class II predicted peptides. Thus, a set of class I and class II specific peptides from FAdV were reported in this study. Hence, a multiepitopic protein was built with these peptides, and subsequently tested to confirm the production of specific antibodies in chicken.
authors.affiliation: Farvet s.A.C. Carretera Panamericana Sur N° 766 Km 198.5, Chincha Alta. Ica - Peru ; Laboratorio de Bioinformática y Biologáa Molecular, Laboratorios de Investigación y Desarrollo, Facultad de Ciencias y Filosofáa, Universidad Peruana Cayetano Heredia, Av. Honorio Delgado 430, San Martin de Porres. Lima - Peru.
authors.forename: Hugo
authors.initials: H
authors.lastname: Valdivia-Olarte
authors.affiliation: Farvet s.A.C. Carretera Panamericana Sur N° 766 Km 198.5, Chincha Alta. Ica - Peru ; Laboratorio de Bioinformática y Biologáa Molecular, Laboratorios de Investigación y Desarrollo, Facultad de Ciencias y Filosofáa, Universidad Peruana Cayetano Heredia, Av. Honorio Delgado 430, San Martin de Porres. Lima - Peru.
authors.forename: David
authors.initials: D
authors.lastname: Requena
authors.affiliation: Farvet s.A.C. Carretera Panamericana Sur N° 766 Km 198.5, Chincha Alta. Ica - Peru ; Laboratorio de Bioinformática y Biologáa Molecular, Laboratorios de Investigación y Desarrollo, Facultad de Ciencias y Filosofáa, Universidad Peruana Cayetano Heredia, Av. Honorio Delgado 430, San Martin de Porres. Lima - Peru.
authors.forename: Manuel
authors.initials: M
authors.lastname: Ramirez
authors.affiliation: Farvet s.A.C. Carretera Panamericana Sur N° 766 Km 198.5, Chincha Alta. Ica - Peru.
authors.forename: Luis E
authors.initials: LE
authors.lastname: Saravia
authors.affiliation: Farvet s.A.C. Carretera Panamericana Sur N° 766 Km 198.5, Chincha Alta. Ica - Peru.
authors.forename: Ray
authors.initials: R
authors.lastname: Izquierdo
authors.affiliation: Farvet s.A.C. Carretera Panamericana Sur N° 766 Km 198.5, Chincha Alta. Ica - Peru.
authors.forename: Francesca
authors.initials: F
authors.lastname: Falconi-Agapito
authors.affiliation: Farvet s.A.C. Carretera Panamericana Sur N° 766 Km 198.5, Chincha Alta. Ica - Peru.
authors.forename: Milagros
authors.initials: M
authors.lastname: Zavaleta
authors.affiliation: Farvet s.A.C. Carretera Panamericana Sur N° 766 Km 198.5, Chincha Alta. Ica - Peru.
authors.forename: Iván
authors.initials: I
authors.lastname: Best
authors.affiliation: Farvet s.A.C. Carretera Panamericana Sur N° 766 Km 198.5, Chincha Alta. Ica - Peru.
authors.forename: Manolo
authors.initials: M
authors.lastname: Fernández-Díaz
authors.affiliation: Farvet s.A.C. Carretera Panamericana Sur N° 766 Km 198.5, Chincha Alta. Ica - Peru ; Laboratorio de Bioinformática y Biologáa Molecular, Laboratorios de Investigación y Desarrollo, Facultad de Ciencias y Filosofáa, Universidad Peruana Cayetano Heredia, Av. Honorio Delgado 430, San Martin de Porres. Lima - Peru.
authors.forename: Mirko
authors.initials: M
authors.lastname: Zimic