title: Formation and Characterization of Supported Lipid Bilayers Composed of Hydrogenated and Deuterated Escherichia coli Lipids.
abstract: Supported lipid bilayers are widely used for sensing and deciphering biomolecular interactions with model cell membranes. In this paper, we present a method to form supported lipid bilayers from total lipid extracts of Escherichia coli by vesicle fusion. We show the validity of this method for different types of extracts including those from deuterated biomass using a combination of complementary surface sensitive techniques; quartz crystal microbalance, neutron reflection and atomic force microscopy. We find that the head group composition of the deuterated and the hydrogenated lipid extracts is similar (approximately 75% phosphatidylethanolamine, 13% phosphatidylglycerol and 12% cardiolipin) and that both samples can be used to reconstitute high-coverage supported lipid bilayers with a total thickness of 41 ± 3 Å, common for fluid membranes. The formation of supported lipid bilayers composed of natural extracts of Escherichia coli allow for following biomolecular interactions, thus advancing the field towards bacterial-specific membrane biomimics.
authors.affiliation: Nano-Science Center and Department of Chemistry, University of Copenhagen, Copenhagen, Denmark.|European Spallation Source ESS AB, Lund, Sweden.
authors.forename: Tania Kjellerup
authors.initials: TK
authors.lastname: Lind
authors.affiliation: Nano-Science Center and Department of Chemistry, University of Copenhagen, Copenhagen, Denmark.|European Spallation Source ESS AB, Lund, Sweden.
authors.forename: Hanna
authors.initials: H
authors.lastname: Wacklin
authors.affiliation: Institute of Medical Physics and Biophysics, Faculty of Medicine, University of Leipzig, Leipzig, Germany.
authors.forename: Jürgen
authors.initials: J
authors.lastname: Schiller
authors.affiliation: Institut Laue-Langevin, Life Science Group, Grenoble, France.
authors.forename: Martine
authors.initials: M
authors.lastname: Moulin
authors.affiliation: Institut Laue-Langevin, Life Science Group, Grenoble, France.
authors.forename: Michael
authors.initials: M
authors.lastname: Haertlein
authors.affiliation: Centre for Membrane Pumps in Cells and Disease-PUMPKIN, Department of Plant and Environmental Sciences, University of Copenhagen, Copenhagen, Denmark.
authors.forename: Thomas Günther
authors.initials: TG
authors.lastname: Pomorski
authors.affiliation: Nano-Science Center and Department of Chemistry, University of Copenhagen, Copenhagen, Denmark.|Malmoe University, Department of Biomedical Sciences, Health & Society, 20500 Malmoe, Sweden.
authors.forename: Marité
authors.initials: M
authors.lastname: Cárdenas
mesh_terms: D002308:Cardiolipins / Q000737:chemistry; D003903:Deuterium / Q000737:chemistry*; D004926:Escherichia coli / Q000737:chemistry*; D006859:Hydrogen / Q000737:chemistry*; D006865:Hydrogenation; D007700:Kinetics; D008051:Lipid Bilayers / Q000737:chemistry*; D008561:Membrane Fusion; D018625:Microscopy, Atomic Force; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010715:Phosphatidylglycerols / Q000737:chemistry; D058525:Quartz Crystal Microbalance Techniques; D013816:Thermodynamics
chemical_list: D002308:Cardiolipins; D008051:Lipid Bilayers; D010714:Phosphatidylethanolamines; D010715:Phosphatidylglycerols; C483858:phosphatidylethanolamine; D006859:Hydrogen; D003903:Deuterium