title: MicroRNA-144 mediates metabolic shift in ovarian cancer cells by directly targeting Glut1.
abstract: Warburg effect is characterized by an increased utilization of glucose via glycolysis in cancer cells, even when enough oxygen is present to properly respire. Recent studies demonstrate that deregulation of microRNAs contributes to the Warburg effect. In the present study, we show that miR-144 is downregulated while glucose transporter 1 (Glut1) is upregulated in ovarian cancers. In vitro studies further showed that miR-144 inhibits Glut1 expression through targeting its 3'-untranslated region. As a result, cells overexpressing miR-144 exhibited a metabolic shift, including enhanced glucose uptake and lactate production. The altered glucose metabolism induced by miR-144 also leads to a rapid growth of ovarian cancer cells. Taken together, our results indicate that miR-144 may serve as a molecular switch to regulate glycolysis in ovarian cancer by targeting the expression of Glut1.
authors.affiliation: Department of gynecology, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, 9th Jinsui Road, Guangzhou, Guangdong, 510623, People's Republic of China.
authors.forename: Jia-Ying
authors.initials: JY
authors.lastname: Fan
authors.affiliation: Department of gynecology, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, 9th Jinsui Road, Guangzhou, Guangdong, 510623, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of gynecology, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, 9th Jinsui Road, Guangzhou, Guangdong, 510623, People's Republic of China.
authors.forename: Jing-Ying
authors.initials: JY
authors.lastname: Xie
authors.affiliation: Department of gynecology, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, 9th Jinsui Road, Guangzhou, Guangdong, 510623, People's Republic of China.
authors.forename: Yan-Ling
authors.initials: YL
authors.lastname: Lu
authors.affiliation: Department of gynecology, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, 9th Jinsui Road, Guangzhou, Guangdong, 510623, People's Republic of China.
authors.forename: Kun
authors.initials: K
authors.lastname: Shi
authors.affiliation: Department of gynecology, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, 9th Jinsui Road, Guangzhou, Guangdong, 510623, People's Republic of China. DrYanqingHuang@163.com.
authors.forename: Yan-Qing
authors.initials: YQ
authors.lastname: Huang
mesh_terms: D020413:3' Untranslated Regions; D000818:Animals; D001665:Binding Sites; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D004195:Disease Models, Animal; D015536:Down-Regulation; D004734:Energy Metabolism; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D005947:Glucose / Q000378:metabolism; D051272:Glucose Transporter Type 1 / Q000235:genetics*; D006019:Glycolysis; D064593:Heterografts; D006801:Humans; D008297:Male; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D034622:RNA Interference; D047368:Tumor Burden
chemical_list: D020413:3' Untranslated Regions; D051272:Glucose Transporter Type 1; C554293:MIRN144 microRNA, human; D035683:MicroRNAs; D005947:Glucose
keywords: Glut1; MicroRNA-144; Ovarian cancer; Warburg effect