title: Resistance and integron characterization of Acinetobacter baumannii in a teaching hospital in Chongqing, China.
abstract: A total of 189 Acinetobacter baumannii isolates were collected in 2011 from a teaching hospital in Chongqing, China. Susceptibility data showed strains carrying integrons were significantly more resistant to all tested antibiotics that strains lacking integrons. Five types of gene cassettes belonging to class I integrons were identified in this study, and for the first time two types of gene cassettes belonging to class II integrons are reported. Most of the cassettes belong to a class I integron (136/144) encoding arr3, aacA4, dfrA17, aadA5, aadB, cat, blaOXA10 , aadA1, aadA2, dfrA and aacC1. Isolates contained a class I gene cassette; AadA2-HP-dfrA was the prevalent strain in this hospital. A class II integron was detected in eight strains, which contained the type IV fimbriae expression regulatory gene pilR and sulfate adenylyltransferase, suggesting a possible role in multidrug resistance. The major epidemic strains from intensive care unit patients belong to international clone 2. In conclusion, the presence of integrons was significantly associated with multiple drug resistance of A. baumannii in this hospital, and class I integron isolates bearing AadA2-HP-dfrA were the prevalent strain in this hospital.
authors.affiliation: Institute of Modern Biopharmaceuticals, State Key Laboratory Breeding Base of Eco-Environment and Bio-Resource of the Three Gorges Area, Key Laboratory of Eco-Environments in Three Gorges Reservoir Region, Ministry of Education, School of Life Sciences, Southwest University, Beibei, China ; The Fifth People's Hospital of Chongqing, Nanan, China.
authors.forename: C
authors.initials: C
authors.lastname: Huang
authors.affiliation: The Key Laboratory of Molecular Biology of Infectious Diseases of the Chinese Ministry of Education, Chongqing Medical University, Yuzhong, Chongqing, China ; Chongqing Center for Clinical Laboratory, The Third People's Hospital of Chongqing, Yuzhong, Chongqing, China.
authors.forename: Q
authors.initials: Q
authors.lastname: Long
authors.affiliation: The Fifth People's Hospital of Chongqing, Nanan, China.
authors.forename: K
authors.initials: K
authors.lastname: Qian
authors.affiliation: Institute of Modern Biopharmaceuticals, State Key Laboratory Breeding Base of Eco-Environment and Bio-Resource of the Three Gorges Area, Key Laboratory of Eco-Environments in Three Gorges Reservoir Region, Ministry of Education, School of Life Sciences, Southwest University, Beibei, China.
authors.forename: T
authors.initials: T
authors.lastname: Fu
authors.affiliation: Institute of Modern Biopharmaceuticals, State Key Laboratory Breeding Base of Eco-Environment and Bio-Resource of the Three Gorges Area, Key Laboratory of Eco-Environments in Three Gorges Reservoir Region, Ministry of Education, School of Life Sciences, Southwest University, Beibei, China ; Chongqing Center for Clinical Laboratory, The Third People's Hospital of Chongqing, Yuzhong, Chongqing, China.
authors.forename: Z
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Chongqing Center for Clinical Laboratory, The Third People's Hospital of Chongqing, Yuzhong, Chongqing, China.
authors.forename: P
authors.initials: P
authors.lastname: Liao
authors.affiliation: Institute of Modern Biopharmaceuticals, State Key Laboratory Breeding Base of Eco-Environment and Bio-Resource of the Three Gorges Area, Key Laboratory of Eco-Environments in Three Gorges Reservoir Region, Ministry of Education, School of Life Sciences, Southwest University, Beibei, China.
authors.forename: J
authors.initials: J
authors.lastname: Xie
keywords: Acinetobacter baumannii; Intensive Care Unit; drug resistance; integron; integron cassettes