title: Identification and molecular mapping of Rps11, a novel gene conferring resistance to Phytophthora sojae in soybean.
abstract: CONCLUSIONS
  Rps11 confers excellent resistance to predominant Phytophthora sojae isolates capable of defeating major Rps genes deployed into soybean production, representing a novel source of resistance for soybean cultivar enhancement.
  Phytophthora root and stem rot (PRSR), caused by the soil-borne pathogen Phytophthora sojae, is a devastating disease of soybean [Glycine max (L.) Merr.] throughout the world. Deploying resistant soybean cultivars is the most effective and environmentally friendly approach to managing this disease. The soybean landrace PI 594527 was found to carry excellent resistance to all P. sojae isolates examined, some of which were capable of overcoming the major Rps genesp, such as Rps1-k, Rps1-c, and Rps3-a, predominantly used for soybean protection in the past decades. A mapping population consisting of 58 F2 individuals and 209 F2:3 families derived from a cross between PI 594527 and the susceptible cultivar 'Williams' was used to characterize the inheritance pattern of the resistance to P. soja (Rps) in PI 594527. It was found that the resistance was conferred by a single Rps gene, designated Rps11, which was initially defined as an ~5 Mb genomic region at the beginning of chromosome 7 by bulked segregant analysis (BSA) with a nucleotide polymorphism (SNP) chip comprising 7039 SNP markers. Subsequently, simple sequence repeat (SSR) markers in the defined region were used to genotype the F2:3 mapping population to map Rps11 to a 225.3 kb genomic region flanked by SSR markers BARCSOYSSR_07_0286 and BARCSOYSSR_07_0300, according to the soybean reference genome sequence. Particularly, an SSR marker (i.e., BARCSOYSSR_07_0295) was found to tightly co-segregate with Rps11 in the mapping population and can be effectively used for marker-assisted selection of this gene for development of resistant soybean cultivars.
authors.affiliation: Department of Agronomy, Purdue University, West Lafayette, IN, 47907, USA.|Bayer CropScience LP, Research Triangle Park, NC, 27709, USA.
authors.forename: Jieqing
authors.initials: J
authors.lastname: Ping
authors.affiliation: Department of Agronomy, Purdue University, West Lafayette, IN, 47907, USA.
authors.forename: Joshua C
authors.initials: JC
authors.lastname: Fitzgerald
authors.affiliation: Department of Agronomy, Purdue University, West Lafayette, IN, 47907, USA.
authors.forename: Chunbao
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Agronomy, Purdue University, West Lafayette, IN, 47907, USA.|Department of Plant, Soil and Microbial Sciences, Michigan State University, East Lansing, MI, 48824, USA.
authors.forename: Feng
authors.initials: F
authors.lastname: Lin
authors.affiliation: Dow AgroSciences LLC., Indianapolis, IN, 46268, USA.|Bayer CropScience LP, Research Triangle Park, NC, 27709, USA.
authors.forename: Yonghe
authors.initials: Y
authors.lastname: Bai
authors.affiliation: Department of Plant, Soil and Microbial Sciences, Michigan State University, East Lansing, MI, 48824, USA.
authors.forename: Dechun
authors.initials: D
authors.lastname: Wang
authors.affiliation: Dow AgroSciences LLC., Indianapolis, IN, 46268, USA.
authors.forename: Rajat
authors.initials: R
authors.lastname: Aggarwal
authors.affiliation: Dow AgroSciences LLC., Indianapolis, IN, 46268, USA.
authors.forename: Maqsood
authors.initials: M
authors.lastname: Rehman
authors.affiliation: Dow AgroSciences LLC., Indianapolis, IN, 46268, USA.
authors.forename: Oswald
authors.initials: O
authors.lastname: Crasta
authors.affiliation: Department of Agronomy, Purdue University, West Lafayette, IN, 47907, USA. maj@purdue.edu.
authors.forename: Jianxin
authors.identifier: http://orcid.org/0000-0002-1474-812X
authors.initials: J
authors.lastname: Ma
mesh_terms: D002874:Chromosome Mapping; D018744:DNA, Plant / Q000235:genetics; D060467:Disease Resistance / Q000235:genetics*; D005799:Genes, Dominant; D017343:Genes, Plant; D060005:Genotyping Techniques; D040582:Inheritance Patterns; D018895:Microsatellite Repeats; D010838:Phytophthora; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D013025:Glycine max / Q000235:genetics* / Q000382:microbiology
chemical_list: D018744:DNA, Plant; D010940:Plant Proteins