title: Desmoplastic melanoma: an updated immunohistochemical analysis of 40 cases with a proposal for an additional panel of stains for diagnosis.
abstract: Desmoplastic melanoma (DM) is histologically characterized by a proliferation of spindle melanocytes dispersed in a collagenous stroma that can be mistaken for a variety of neoplasms. The purpose of this study was to analyze 40 cases of DM with a comprehensive panel of immunohistochemical markers (KBA.62, p16, Ezrin, WT-1, MITF-1, SOX-10, CD117, SOX-2, nestin, PNL2, p75, MART-1, gp100 and S100p) to obtain a more complete understanding of the potential use of these antibodies in the diagnosis of DM. We found that all cases of DM expressed p16, WT-1, SOX-10, nestin and S100p and 95% of cases expressed p75. There was variable expression with Ezrin, SOX-2, KBA.62, MART-1 and HMB-45. Most DMs did not express MITF-1, PNL2 and CD117. Conditions that may enter in the histologic differential diagnosis of DM, including dermal scars, fibromatosis and dermatofibromas were also studied. Nearly all control cases also stained positive for p16 but were negative for WT1, SOX10, nestin, p75 and S-100p, as well as for most of the other markers tested. We conclude that a panel of S-100p, WT1, SOX10, p75 and nestin may constitute the optimal panel with the most sensitive and specific combination of immunostain available for the diagnosis of DM.
authors.affiliation: Dermatopathology, Medical College of Wisconsin, Milwaukee, WI, USA.
authors.forename: Jose A
authors.initials: JA
authors.lastname: Plaza
authors.affiliation: Pathology, Medical College of Wisconsin, Milwaukee, WI, USA.
authors.forename: Peter
authors.initials: P
authors.lastname: Bonneau
authors.affiliation: Pathology and Dermatology, UT-M.D. Anderson Cancer Center, Houston, TX, USA.
authors.forename: Victor
authors.initials: V
authors.lastname: Prieto
authors.affiliation: Pathology and Dermatology, Hospital Obrero, La Paz, Plurinational State of Bolivia.
authors.forename: Martin
authors.initials: M
authors.lastname: Sangueza
authors.affiliation: Pathology, Medical College of Wisconsin, Milwaukee, WI, USA.
authors.forename: Alexander
authors.initials: A
authors.lastname: Mackinnon
authors.affiliation: Pathology, Medical College of Wisconsin, Milwaukee, WI, USA.
authors.forename: David
authors.initials: D
authors.lastname: Suster
authors.affiliation: Pathology, Bacchi Laboratory, Botucatu, Brazil.
authors.forename: Carlos
authors.initials: C
authors.lastname: Bacchi
authors.affiliation: Pathology, Bacchi Laboratory, Botucatu, Brazil.
authors.forename: Bruna
authors.initials: B
authors.lastname: Estrozi
authors.affiliation: Pathology, Charles University, Medical Faculty Hospital, Pilsen, Czech Republic.
authors.forename: Dmitry
authors.initials: D
authors.lastname: Kazakov
authors.affiliation: Pathology, Charles University, Medical Faculty Hospital, Pilsen, Czech Republic.
authors.forename: Denisa
authors.initials: D
authors.lastname: Kacerovska
authors.affiliation: Pathology, General Hospital S. Maria Della Misericordia, Udine, Italy.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Falconieri
authors.affiliation: Pathology, Medical College of Wisconsin, Milwaukee, WI, USA.
authors.forename: Saul
authors.initials: S
authors.lastname: Suster
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008545:Melanoma / Q000378:metabolism* / Q000473:pathology*; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000378:metabolism*; D012878:Skin Neoplasms / Q000378:metabolism* / Q000473:pathology*; D013194:Staining and Labeling / Q000379:methods
chemical_list: D009363:Neoplasm Proteins
keywords: desmoplastic melanoma; immunohistochemistry; melanoma; spindle cell melanoma