title: Scaffold-cell bone engineering in a validated preclinical animal model: precursors vs differentiated cell source.
abstract: The properties of osteoblasts (OBs) isolated from the axial skeleton (tOBs) differ from OBs of the orofacial skeleton (mOBs) due to the different embryological origins of the bones. The aim of the study was to assess and compare the regenerative potential of allogenic bone marrow-derived mesenchymal progenitor cells with allogenic tOBs and allogenic mOBs in combination with a mPCL-TCP scaffold in critical-sized segmental bone defects in sheep tibiae. After 6 months, the tibiae were explanted and underwent biomechanical testing, micro-computed tomography (microCT) and histological and immunohistochemical analyses. Allogenic MPCs demonstrated a trend towards a better outcome in biomechanical testing and the mean values of newly formed bone. Biomechanical, microCT and histological analysis showed no significant differences in the bone regeneration potential of tOBs and mOBs in our in vitro study, as well as in the bone regeneration potential of different cell types in vivo. Copyright © 2015 John Wiley & Sons, Ltd.
authors.affiliation: Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Queensland, Australia.|Department of Trauma Surgery, University of Regensburg, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Berner
authors.affiliation: Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Queensland, Australia.
authors.forename: J
authors.initials: J
authors.lastname: Henkel
authors.affiliation: Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Queensland, Australia.
authors.forename: M A
authors.initials: MA
authors.lastname: Woodruff
authors.affiliation: Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Queensland, Australia.
authors.forename: S
authors.initials: S
authors.lastname: Saifzadeh
authors.affiliation: Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Queensland, Australia.
authors.forename: G
authors.initials: G
authors.lastname: Kirby
authors.affiliation: Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Queensland, Australia.|Department of Trauma Surgery, University of Regensburg, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Zaiss
authors.affiliation: Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Queensland, Australia.
authors.forename: J
authors.initials: J
authors.lastname: Gohlke
authors.affiliation: Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Queensland, Australia.|Department of Orthopaedics and Accident Surgery, Waldkrankenhaus Protestant Hospital, Berlin, Germany.
authors.forename: J C
authors.initials: JC
authors.lastname: Reichert
authors.affiliation: Department of Trauma Surgery, University of Regensburg, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Nerlich
authors.affiliation: Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Queensland, Australia.
authors.forename: M A
authors.initials: MA
authors.lastname: Schuetz
authors.affiliation: Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Queensland, Australia.
authors.forename: D W
authors.initials: DW
authors.lastname: Hutmacher
mesh_terms: D064591:Allografts; D000818:Animals; D001861:Bone Regeneration; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D010006:Osteoblasts / Q000378:metabolism / Q000637:transplantation; D010012:Osteogenesis; D012756:Sheep; D013977:Tibia / Q000000981:diagnostic imaging / Q000293:injuries* / Q000378:metabolism*; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds; D055114:X-Ray Microtomography
keywords: allogenic cells; bone defect; bone regeneration; bone tissue engineering; mesenchymal progenitor cells; osteoblasts; polycaprolactone; scaffolds