title: Enhancement of CD3AK cell proliferation and killing ability by α-Thujone.
abstract: Thujone is a monoterpene ketone natural substance found mainly in wormwood and sage. Previous studies have shown that Thujone has various pharmacological effects, such as anti-tumor, analgesic, and insecticide. The effect of α-Thujone to human immune cells is still unknown. Our study focuses on investigating the effects and mechanism of α-Thujone to CD3AK (anti- CD3 antibody induced activated killer) cells proliferation and cytotoxicity to colon cancer cell lines. With cell proliferation and FCM assay, it is found that α-Thujone could significantly enhance CD3AK cell proliferation and expression of CD107a in a dose-dependent manner. The cytotoxicity to colon cancer cells detected by CCK-8 assay is also improved. The expressions of TNF-α and FasL detected with ELISA assay were not significantly changed. Mechanically, the study shows that α-Thujone could enhance the expression of p-ERK1/2 and p-Akt. In addition, α-Thujone has no cytotoxicity to HCT116 and SW620 cells proliferation. In a word, α-Thujone enhances CD3AK cell proliferation and cytotoxicity via the improvement of expression of CD107a, p-Akt and p-ERK1/2.
authors.affiliation: Department of Central Laboratory, 97th Hospital of PLA, 226 Tongshan Road, Xuzhou 221004, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Department of Central Laboratory, 97th Hospital of PLA, 226 Tongshan Road, Xuzhou 221004, China.
authors.forename: Jun-Quan
authors.initials: JQ
authors.lastname: Liu
authors.affiliation: Department of Central Laboratory, 97th Hospital of PLA, 226 Tongshan Road, Xuzhou 221004, China.
authors.forename: Zhong-Hai
authors.initials: ZH
authors.lastname: Zhou
authors.affiliation: Department of Central Laboratory, 97th Hospital of PLA, 226 Tongshan Road, Xuzhou 221004, China.
authors.forename: Xiao-Ting
authors.initials: XT
authors.lastname: Lv
authors.affiliation: Department of Central Laboratory, 97th Hospital of PLA, 226 Tongshan Road, Xuzhou 221004, China.
authors.forename: Yong-Qiang
authors.initials: YQ
authors.lastname: Chen
authors.affiliation: Department of Central Laboratory, 97th Hospital of PLA, 226 Tongshan Road, Xuzhou 221004, China.
authors.forename: Lei-Qing
authors.initials: LQ
authors.lastname: Sun
authors.affiliation: Department of Central Laboratory, 97th Hospital of PLA, 226 Tongshan Road, Xuzhou 221004, China. Electronic address: chenfuxingtitc@163.com.
authors.forename: Fu-Xing
authors.initials: FX
authors.lastname: Chen
mesh_terms: D000906:Antibodies / Q000378:metabolism; D019306:Artemisia / Q000276:immunology; D000081008:Bicyclic Monoterpenes; D017252:CD3 Complex / Q000276:immunology; D019496:Cancer Vaccines / Q000276:immunology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D003110:Colonic Neoplasms / Q000276:immunology / Q000628:therapy*; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D006801:Humans; D016219:Immunotherapy, Adoptive; D052118:Lysosomal-Associated Membrane Protein 1 / Q000378:metabolism; D020935:MAP Kinase Signaling System / Q000187:drug effects; D016260:Monocytes, Activated Killer / Q000187:drug effects* / Q000502:physiology; D039821:Monoterpenes / Q000494:pharmacology*; D010766:Phosphorylation / Q000187:drug effects; D051057:Proto-Oncogene Proteins c-akt / Q000235:genetics / Q000378:metabolism; D027543:Salvia officinalis / Q000276:immunology
chemical_list: D000906:Antibodies; D000081008:Bicyclic Monoterpenes; D017252:CD3 Complex; D019496:Cancer Vaccines; D052118:Lysosomal-Associated Membrane Protein 1; D039821:Monoterpenes; C005790:beta-thujone; D051057:Proto-Oncogene Proteins c-akt
keywords: CD3AK cells; Cytotoxicity; Human colon cancer cell; α­Thujone