title: Rapid Quantification of Four Anthocyanins in Red Grape Wine by Hydrophilic Interaction Liquid Chromatography/Triple Quadrupole Linear Ion Trap Mass Spectrometry.
abstract: The identification and quantification of four anthocyanins (cyanidin-3-O-glucoside, peonidin-3-O-glucoside, delphinidin-3-O-glucoside, and malvidin-3-O-glucoside) in red grape wine were carried out by hydrophilic interaction liquid chromatography/triple quadrupole linear ion trap MS (HILIC/QTrap-MS/MS). Samples were diluted directly and separated on a Merck ZIC HILIC column with 20 mM ammonium acetate solution-acetonitrile mobile phase. Quantitative data acquisition was carried out in the multiple reaction monitoring mode. Additional identification and confirmation of target compounds were performed using the enhanced product ion mode of the linear ion trap. The LOQs were in the range 0.05-1.0 ng/mL. The average recoveries were in the range 94.6 to 104.5%. The HILIC/QTrap-MS/MS platform offers the best sensitivity and specificity for characterization and quantitative determination of the four anthocyanins in red grape wines and fulfills the quality criteria for routine laboratory application.
authors.affiliation: Faculty of Life Science and Chemical Engineering, Huaiyin Institute of Technology, Huaian 223003, People's Republic of China.
authors.forename: Yongming
authors.initials: Y
authors.lastname: Sun
authors.forename: Biqi
authors.initials: B
authors.lastname: Xia
authors.forename: Xiangzhun
authors.initials: X
authors.lastname: Chen
authors.forename: Chuansong
authors.initials: C
authors.lastname: Duanmu
authors.forename: Denghao
authors.initials: D
authors.lastname: Li
authors.forename: Chao
authors.initials: C
authors.lastname: Han
mesh_terms: D000872:Anthocyanins / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods*; D057927:Hydrophobic and Hydrophilic Interactions; D007477:Ions / Q000737:chemistry; D057230:Limit of Detection; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013997:Time Factors; D027843:Vitis / Q000737:chemistry*; D014920:Wine / Q000032:analysis*
chemical_list: D000872:Anthocyanins; D007477:Ions