title: Benzoate degradation by Rhodococcus opacus 1CP after dormancy: Characterization of dioxygenases involved in the process.
abstract: The process of benzoate degradation by strain Rhodococcus opacus 1CP after a five-year dormancy was investigated and its peculiarities were revealed. The strain was shown to be capable of growth on benzoate at a concentration of up to 10 g L(-1). The substrate specificity of benzoate dioxygenase (BDO) during the culture growth on a medium with a low (200-250 mg L(-1)) and high (4 g L(-1)) concentration of benzoate was assessed. BDO of R. opacus 1CP was shown to be an extremely narrow specificity enzyme. Out of 31 substituted benzoates, only with one, 3-chlorobenzoate, its activity was higher than 9% of that of benzoate. Two dioxygenases, catechol 1,2-dioxygenase (Cat 1,2-DO) and protocatechuate 3,4-dioxygenase (PCA 3,4-DO), were identified in a cell-free extract, purified and characterized. The substrate specificity of Cat 1,2-DO isolated from cells of strain 1CP after the dormancy was found to differ significantly from that of Cat 1,2-DO isolated earlier from cells of this strain grown on benzoate. By its substrate specificity, the described Cat 1,2-DO was close to the Cat 1,2-DO from strain 1CP grown on 4-methylbenzoate. Neither activity nor inhibition by protocatechuate was observed during the reaction of Cat 1,2-DO with catechol, and catechol had no inhibitory effect on the reaction of PCA 3,4-DO with protocatechuate.
authors.affiliation: a FSBIS G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences , Pushchino , Russia.
authors.forename: Inna P
authors.initials: IP
authors.lastname: Solyanikova
authors.affiliation: a FSBIS G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences , Pushchino , Russia.
authors.forename: Elena V
authors.initials: EV
authors.lastname: Emelyanova
authors.affiliation: a FSBIS G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences , Pushchino , Russia.|b Pushchino State Natural Science Institute , Pushchino , Russia.
authors.forename: Oksana V
authors.initials: OV
authors.lastname: Borzova
authors.affiliation: a FSBIS G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences , Pushchino , Russia.|b Pushchino State Natural Science Institute , Pushchino , Russia.
authors.forename: Ludmila A
authors.initials: LA
authors.lastname: Golovleva
mesh_terms: D001565:Benzoates / Q000378:metabolism; D001673:Biodegradation, Environmental; D050548:Catechol 1,2-Dioxygenase / Q000378:metabolism; D002396:Catechols / Q000378:metabolism; D002474:Cell-Free System; D002723:Chlorobenzoates / Q000378:metabolism; D049308:Dioxygenases / Q000378:metabolism*; D062385:Hydroxybenzoates / Q000378:metabolism; D011520:Protocatechuate-3,4-Dioxygenase / Q000378:metabolism; D012240:Rhodococcus / Q000378:metabolism* / Q000502:physiology; D013379:Substrate Specificity
chemical_list: D001565:Benzoates; D002396:Catechols; D002723:Chlorobenzoates; D062385:Hydroxybenzoates; C036427:3-chlorobenzoic acid; C009091:protocatechuic acid; C009504:4-toluic acid; D049308:Dioxygenases; D050548:Catechol 1,2-Dioxygenase; D011520:Protocatechuate-3,4-Dioxygenase; C034221:catechol
keywords: Rhodococcus opacus 1CP; benzoate; benzoate dioxygenase; catechol 1,2-dioxygenase; protocatechuate 3,4-dioxygenase; substrate specificity