title: Pepino mosaic virus RNA-Dependent RNA Polymerase POL Domain Is a Hypersensitive Response-Like Elicitor Shared by Necrotic and Mild Isolates.
abstract: Pepino mosaic virus (PepMV) is an emerging pathogen that represents a serious threat to tomato production worldwide. PepMV-induced diseases manifest with a wide range of symptoms, including systemic necrosis. Our results showed that PepMV accumulation depends on the virus isolate, tomato cultivar, and environmental conditions, and associates with the development of necrosis. Substitution of lysine for glutamic acid at position 67 in the triple gene block 3 (TGB3) protein, previously described as a necrosis determinant, led to increased virus accumulation and was necessary but not sufficient to induce systemic necrosis. Systemic necrosis both in tomato and Nicotiana benthamiana shared hypersensitive response (HR) features, allowing the assessment of the role of different genomic regions on necrosis induction. Overexpression of both TGB3 and the polymerase domain (POL) of the RNA-dependent RNA polymerase (RdRp) resulted in necrosis, although only local expression of POL triggered HR-like symptoms. Our results also indicated that the necrosis-eliciting activity of POL resides in its highly conserved "palm" domain, and that necrosis was jasmonic acid-dependent but not salicylic acid-dependent. Altogether, our data suggest that the RdRp-POL domain plays an important role in PepMV necrosis induction, with necrosis development depending on the virus accumulation level, which can be modulated by the nature of TGB3, host genotype and environmental conditions.
authors.affiliation: First, second, third, fourth, sixth, and seventh authors: Centro de Edafología y Biología Aplicada del Segura (CEBAS), Consejo Superior de Investigaciones Científicas (CSIC), P.O. Box 164, 30100 Espinardo, Murcia, Spain; and fifth author: Institute of Plant Protection-National Research Institute, Department of Virology and Bacteriology, ul. Władysława Węgorka 20, 60-318 Poznán (Poland).
authors.forename: Raquel N
authors.initials: RN
authors.lastname: Sempere
authors.affiliation: First, second, third, fourth, sixth, and seventh authors: Centro de Edafología y Biología Aplicada del Segura (CEBAS), Consejo Superior de Investigaciones Científicas (CSIC), P.O. Box 164, 30100 Espinardo, Murcia, Spain; and fifth author: Institute of Plant Protection-National Research Institute, Department of Virology and Bacteriology, ul. Władysława Węgorka 20, 60-318 Poznán (Poland).
authors.forename: Cristina
authors.initials: C
authors.lastname: Gómez-Aix
authors.affiliation: First, second, third, fourth, sixth, and seventh authors: Centro de Edafología y Biología Aplicada del Segura (CEBAS), Consejo Superior de Investigaciones Científicas (CSIC), P.O. Box 164, 30100 Espinardo, Murcia, Spain; and fifth author: Institute of Plant Protection-National Research Institute, Department of Virology and Bacteriology, ul. Władysława Węgorka 20, 60-318 Poznán (Poland).
authors.forename: Fabiola
authors.initials: F
authors.lastname: Ruíz-Ramón
authors.affiliation: First, second, third, fourth, sixth, and seventh authors: Centro de Edafología y Biología Aplicada del Segura (CEBAS), Consejo Superior de Investigaciones Científicas (CSIC), P.O. Box 164, 30100 Espinardo, Murcia, Spain; and fifth author: Institute of Plant Protection-National Research Institute, Department of Virology and Bacteriology, ul. Władysława Węgorka 20, 60-318 Poznán (Poland).
authors.forename: Pedro
authors.initials: P
authors.lastname: Gómez
authors.affiliation: First, second, third, fourth, sixth, and seventh authors: Centro de Edafología y Biología Aplicada del Segura (CEBAS), Consejo Superior de Investigaciones Científicas (CSIC), P.O. Box 164, 30100 Espinardo, Murcia, Spain; and fifth author: Institute of Plant Protection-National Research Institute, Department of Virology and Bacteriology, ul. Władysława Węgorka 20, 60-318 Poznán (Poland).
authors.forename: Beata
authors.initials: B
authors.lastname: Hasiów-Jaroszewska
authors.affiliation: First, second, third, fourth, sixth, and seventh authors: Centro de Edafología y Biología Aplicada del Segura (CEBAS), Consejo Superior de Investigaciones Científicas (CSIC), P.O. Box 164, 30100 Espinardo, Murcia, Spain; and fifth author: Institute of Plant Protection-National Research Institute, Department of Virology and Bacteriology, ul. Władysława Węgorka 20, 60-318 Poznán (Poland).
authors.forename: María Amelia
authors.initials: MA
authors.lastname: Sánchez-Pina
authors.affiliation: First, second, third, fourth, sixth, and seventh authors: Centro de Edafología y Biología Aplicada del Segura (CEBAS), Consejo Superior de Investigaciones Científicas (CSIC), P.O. Box 164, 30100 Espinardo, Murcia, Spain; and fifth author: Institute of Plant Protection-National Research Institute, Department of Virology and Bacteriology, ul. Władysława Węgorka 20, 60-318 Poznán (Poland).
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Aranda
mesh_terms: D000595:Amino Acid Sequence; D003517:Cyclopentanes / Q000378:metabolism; D004777:Environment; D005838:Genotype; D054884:Host-Pathogen Interactions; D018551:Solanum lycopersicum / Q000235:genetics / Q000648:ultrastructure / Q000821:virology*; D008969:Molecular Sequence Data; D054883:Oxylipins / Q000378:metabolism; D010935:Plant Diseases / Q000821:virology*; D010937:Plant Growth Regulators / Q000378:metabolism; D018515:Plant Leaves / Q000235:genetics / Q000648:ultrastructure / Q000821:virology; D017863:Potexvirus / Q000201:enzymology* / Q000235:genetics / Q000472:pathogenicity / Q000648:ultrastructure; D017434:Protein Structure, Tertiary; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics*; D020156:Salicylic Acid / Q000378:metabolism; D016415:Sequence Alignment; D014026:Nicotiana / Q000235:genetics / Q000648:ultrastructure / Q000821:virology
chemical_list: D003517:Cyclopentanes; D054883:Oxylipins; D010937:Plant Growth Regulators; C011006:jasmonic acid; D012324:RNA-Dependent RNA Polymerase; D020156:Salicylic Acid
keywords: Solanum lycopersicum; potexvirus; symptom induction