title: Integrative miRNA and whole-genome analyses of epicardial adipose tissue in patients with coronary atherosclerosis.
abstract: BACKGROUND
  Epicardial adipose tissue (EAT) is an atypical fat depot surrounding the heart with a putative role in the development of atherosclerosis.
  
  
  RESULTS
  We profiled genes and miRNAs in perivascular EAT and subcutaneous adipose tissue (SAT) of metabolically healthy patients without coronary artery disease (CAD) vs. metabolic patients with CAD. Compared with SAT, a specific tuning of miRNAs and genes points to EAT as a tissue characterized by a metabolically active and pro-inflammatory profile. Then, we depicted both miRNA and gene signatures of EAT in CAD, featuring a down-regulation of genes involved in lipid metabolism, mitochondrial function, nuclear receptor transcriptional activity, and an up-regulation of those involved in antigen presentation, chemokine signalling, and inflammation. Finally, we identified miR-103-3p as candidate modulator of CCL13 in EAT, and a potential biomarker role for the chemokine CCL13 in CAD.
  
  
  CONCLUSIONS
  EAT in CAD is characterized by changes in the regulation of metabolism and inflammation with miR-103-3p/CCL13 pair as novel putative actors in EAT function and CAD.
authors.affiliation: Department of Interdisciplinary Medicine, University of Bari 'Aldo Moro', Piazza Giulio Cesare 11, 70124 Bari, Italy Fondazione Mario Negri Sud, Santa Maria Imbaro (CH), Italy Ageing Research Center (CeSI), 'G. d'Annunzio' University Foundation, Chieti, Italy.
authors.forename: Michele
authors.initials: M
authors.lastname: Vacca
authors.affiliation: Cardiovascular Department, 'S.Orsola Malpighi' Hospital, University of Bologna, Bologna, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Di Eusanio
authors.affiliation: Department of Interdisciplinary Medicine, University of Bari 'Aldo Moro', Piazza Giulio Cesare 11, 70124 Bari, Italy National Cancer Research Center IRCCS 'Giovanni Paolo II', Bari, Italy.
authors.forename: Marica
authors.initials: M
authors.lastname: Cariello
authors.affiliation: Department of Interdisciplinary Medicine, University of Bari 'Aldo Moro', Piazza Giulio Cesare 11, 70124 Bari, Italy National Cancer Research Center IRCCS 'Giovanni Paolo II', Bari, Italy.
authors.forename: Giusi
authors.initials: G
authors.lastname: Graziano
authors.affiliation: Department of Interdisciplinary Medicine, University of Bari 'Aldo Moro', Piazza Giulio Cesare 11, 70124 Bari, Italy Fondazione Mario Negri Sud, Santa Maria Imbaro (CH), Italy National Cancer Research Center IRCCS 'Giovanni Paolo II', Bari, Italy.
authors.forename: Simona
authors.initials: S
authors.lastname: D'Amore
authors.affiliation: Cardiovascular Department, 'S.Orsola Malpighi' Hospital, University of Bologna, Bologna, Italy.
authors.forename: Francesco Dimitri
authors.initials: FD
authors.lastname: Petridis
authors.affiliation: Fondazione Mario Negri Sud, Santa Maria Imbaro (CH), Italy.
authors.forename: Andria
authors.initials: A
authors.lastname: D'orazio
authors.affiliation: Fondazione Mario Negri Sud, Santa Maria Imbaro (CH), Italy.
authors.forename: Lorena
authors.initials: L
authors.lastname: Salvatore
authors.affiliation: Fondazione Mario Negri Sud, Santa Maria Imbaro (CH), Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Tamburro
authors.affiliation: Cardiovascular Department, 'S.Orsola Malpighi' Hospital, University of Bologna, Bologna, Italy.
authors.forename: Gianluca
authors.initials: G
authors.lastname: Folesani
authors.affiliation: Unit of Cardiology, 'San Paolo' Hospital, Bari, Italy.
authors.forename: David
authors.initials: D
authors.lastname: Rutigliano
authors.affiliation: Fondazione Mario Negri Sud, Santa Maria Imbaro (CH), Italy.
authors.forename: Fabio
authors.initials: F
authors.lastname: Pellegrini
authors.affiliation: Department of Interdisciplinary Medicine, University of Bari 'Aldo Moro', Piazza Giulio Cesare 11, 70124 Bari, Italy.
authors.forename: Carlo
authors.initials: C
authors.lastname: Sabbà
authors.affiliation: Department of Interdisciplinary Medicine, University of Bari 'Aldo Moro', Piazza Giulio Cesare 11, 70124 Bari, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Palasciano
authors.affiliation: Cardiovascular Department, 'S.Orsola Malpighi' Hospital, University of Bologna, Bologna, Italy.
authors.forename: Roberto
authors.initials: R
authors.lastname: Di Bartolomeo
authors.affiliation: Department of Interdisciplinary Medicine, University of Bari 'Aldo Moro', Piazza Giulio Cesare 11, 70124 Bari, Italy Fondazione Mario Negri Sud, Santa Maria Imbaro (CH), Italy National Cancer Research Center IRCCS 'Giovanni Paolo II', Bari, Italy antonio.moschetta@uniba.it.
authors.forename: Antonio
authors.initials: A
authors.lastname: Moschetta
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D050197:Atherosclerosis / Q000235:genetics*; D018925:Chemokines / Q000378:metabolism; D005786:Gene Expression Regulation / Q000235:genetics*; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D015894:Genome, Human; D055106:Genome-Wide Association Study; D006801:Humans; D007249:Inflammation / Q000235:genetics; D050356:Lipid Metabolism / Q000235:genetics*; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged
chemical_list: D018925:Chemokines; D035683:MicroRNAs
keywords: Epicardial adipose tissue; Gene expression; Metabolic syndrome; Nuclear receptors; miRNA