title: An Unprecedented Occurrence of Δ5,9- and Δ9,15-Dienoic Fatty Acids in Ovaries of the Archaeogastropod Limpet Cellana toreuma.
abstract: A detailed structural diversity of dienoic fatty acids (FA), including non-methylene-interrupted dienoic FA, of triacylglycerols and polar lipids in ovaries of Cellana toreuma was clarified for the first time by using capillary gas chromatography-mass spectrometry of their 3-pyridylcarbinol esters and argentation thin-layer chromatography. Interestingly, in addition to 5,9-octadecadienoic (18:2Δ5,9), 5,9-eicosadienoic (20:2Δ5,9), 5,9-heneicosadienoic (21:2Δ5,9), 5,9-docosadienoic (22:2Δ5,9), 5,9-tricosadienoic (23:2Δ5,9), and 5,9-tetracosadienoic (24:2Δ5,9) acids, previously identified in ovaries of C. grata, rare FA 5,9-hexadecadienoic (16:2Δ5,9), 5,9-nonadecadienoic (19:2Δ5,9), and 21-methyl-5,9-docosadienoic (iso 23:2Δ5,9) were newly recognized in ovaries of C. toreuma. Detectable amounts of four Δ9,15-dienoic FA were present in the ovary lipids. The FA identified were one novel 9,15-heneicosadienoic (21:2Δ9,15) acid and known 9,15-docosadienoic (22:2Δ9,15), 9,15-tricosadienoic (23:2Δ9,15), and 9,15-tetracosadienoic (24:2Δ9,15) acids. The findings help to explain the broad evidence of the structural diversity in marine gastropods and suggest biomarkers to evaluate marine food web relations.
authors.affiliation: Bioscience Laboratory, Miyako College, Iwate Prefectural University, Miyako, 027-0039, Japan. ajoe@iwate-pu.ac.jp.
authors.forename: Hideki
authors.initials: H
authors.lastname: Kawashima
authors.affiliation: Department of Food Science and Human Nutrition, Faculty of Human Life Sciences, Fuji Women's University, Ishikari, 061-3204, Japan.
authors.forename: Masao
authors.initials: M
authors.lastname: Ohnishi
mesh_terms: D000818:Animals; D002855:Chromatography, Thin Layer; D004952:Esters / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry* / Q000145:classification / Q000302:isolation & purification; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D049851:Gastropoda / Q000737:chemistry; D010053:Ovary / Q000737:chemistry*; D014280:Triglycerides / Q000737:chemistry* / Q000145:classification
chemical_list: D004952:Esters; D005227:Fatty Acids; D014280:Triglycerides
keywords: Archaeogastropods; Limpet; Non-methylene-interrupted fatty acid; Ovary; Δ5,9-Dienoic fatty acid; Δ9,15-Dienoic fatty acid