title: Microvirga makkahensis sp. nov., and Microvirga arabica sp. nov., isolated from sandy arid soil.
abstract: The taxonomic positions of two Gram-negative strains, SV1470(T) and SV2184P(T), isolated from arid soil samples, were determined using a polyphasic approach. Analysis of the 16S rRNA gene and the concatenated sequences of three housekeeping gene loci (dnaK, rpoB and gyrB) confirmed that the strains belong to the genus Microvirga. Strain SV1470(T) was found to be closely related to Microvirga vignae BR3299(T) (98.8 %), Microvirga flocculans TFB(T) (98.3 %) and Microvirga lupini Lut6(T) (98.2 %), whilst similarity to other type strains of the genus ranged from 97.8 to 96.3 %; strain SV2184P(T) was found to be closely related to Microvirga aerilata 5420S-16(T) (98.0 %), Microvirga zambiensis WSM3693(T) (97.8 %) and M. flocculans ATCC BAA-817(T) (97.4 %), whilst similarity to other type strains of the genus ranged from 97.2 to 95.9 %. The G + C content of the genomic DNA was determined to be 61.5 mol % for strain SV1470(T) and 62.1 mol % for strain SV2184P(T). Both strains were found to have the same quinone system, with Q-10 as the major ubiquinone. The polar lipid profile of strain SV1470(T) was found to consist of phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, one unidentified phospholipid and one unidentified aminolipid, while that of strain SV2184P(T) consisted of phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, one unidentified aminolipid, one unidentified aminophospholipid and two unidentified phospholipids. DNA-DNA relatedness studies showed that the two strains belong to different genomic species. The strains were also distinguished using a combination of phenotypic properties. Based on the genotypic and phenotypic data, the novel species Microvirga makkahensis sp. nov. (type strain SV1470(T) = DSM 25394(T) = KCTC 23863(T) = NRRL-B 24875(T)) and Microvirga arabica sp. nov. (type strain SV2184P(T) = DSM 25393(T) = KCTC 23864(T) = NRRL-B 24874(T)) are proposed.
authors.affiliation: Department of Biology, Faculty of Art and Science, Ondokuz Mayis University, 55139, Atakum-Samsun, Turkey.|Department of Bioengineering, Faculty of Engineering and Architecture, Sinop University, 57000, Nasuhbasoglu-Sinop, Turkey.
authors.forename: Aysel
authors.initials: A
authors.lastname: Veyisoglu
authors.affiliation: Department of Biology, Faculty of Art and Science, Ondokuz Mayis University, 55139, Atakum-Samsun, Turkey.
authors.forename: Demet
authors.initials: D
authors.lastname: Tatar
authors.affiliation: Department of Biology, Faculty of Art and Science, Ondokuz Mayis University, 55139, Atakum-Samsun, Turkey.
authors.forename: Hayrettin
authors.initials: H
authors.lastname: Saygin
authors.affiliation: Molecular Biology and Genetics Department, Faculty of Science, Karadeniz Technical University, 61080, Trabzon, Turkey.
authors.forename: Kadriye
authors.initials: K
authors.lastname: Inan
authors.affiliation: Science Teaching Programme, Gazi Faculty of Education, Gazi University, 06500, Ankara, Turkey.
authors.forename: Demet
authors.initials: D
authors.lastname: Cetin
authors.affiliation: Department of Biology, Faculty of Science, Anadolu University, 26470, Eskisehir, Turkey.
authors.forename: Kiymet
authors.initials: K
authors.lastname: Guven
authors.affiliation: Department of Biology, Faculty of Art and Science, Mersin University, 33342, Ciftlikköy-Mersin, Turkey.
authors.forename: Munir
authors.initials: M
authors.lastname: Tuncer
authors.affiliation: Department of Biology, Faculty of Art and Science, Ondokuz Mayis University, 55139, Atakum-Samsun, Turkey. nsahin@omu.edu.tr.
authors.forename: Nevzat
authors.initials: N
authors.lastname: Sahin
mesh_terms: D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism; D042082:Methylobacteriaceae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D012987:Soil
keywords: Alphaproteobacteria; Microvirga arabica; Microvirga makkahensis; Polyphasic taxonomy; Proteobacteria