title: CRIM1, a newfound cancer-related player, regulates the adhesion and migration of lung cancer cells.
abstract: CRIM1 is a member of the bone morphogenetic protein (BMP) antagonists; however, the role of CRIM1 in controlling cancer cell behavior remains unknown. This study investigated its function in the A549 cell line in vitro. The results show that treating cells with CRIM1 peptide could increase the migration and adhesion of A549. Consistently, silencing the CRIM1 expression decreased the migration and adhesion of A549. Furthermore, the CRIM1 protein expression was increased in A549 which were treated with transforming growth factor beta 1 to induced EMT. However, CRIM1 peptide treatment could increase the expression of N-CAD and E-CAD expression. Finally, overexpression of the oncogene YAP1 resulted in an up-regulation of the CRIM1 expression in A549, suggesting that CRIM1 was a target of the Hippo pathway. These observations provide evidence for the first time that CRIM1 plays a role in cancer cells by enhancing the migration and adhesion and increasing the expression of N-CAD and E-CAD.
authors.affiliation: a Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University , Chongqing , China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zeng
authors.affiliation: a Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University , Chongqing , China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: a Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University , Chongqing , China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Yi
authors.affiliation: a Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University , Chongqing , China.
authors.forename: Yanjiao
authors.initials: Y
authors.lastname: Wu
authors.affiliation: a Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University , Chongqing , China.
authors.forename: Rongxue
authors.initials: R
authors.lastname: Wan
authors.affiliation: a Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University , Chongqing , China.
authors.forename: Liling
authors.initials: L
authors.lastname: Tang
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000096:biosynthesis; D015703:Antigens, CD / Q000096:biosynthesis; D052004:Bone Morphogenetic Protein Receptors; D019485:Bone Morphogenetic Proteins / Q000037:antagonists & inhibitors; D015820:Cadherins / Q000096:biosynthesis; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics / Q000473:pathology*; D002448:Cell Adhesion / Q000187:drug effects / Q000235:genetics*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects / Q000235:genetics*; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000473:pathology*; D008565:Membrane Proteins / Q000235:genetics* / Q000494:pharmacology; D010750:Phosphoproteins / Q000096:biosynthesis; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D015398:Signal Transduction / Q000187:drug effects; D014157:Transcription Factors; D000091102:YAP-Signaling Proteins
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D015703:Antigens, CD; D019485:Bone Morphogenetic Proteins; C050361:CDH2 protein, human; C486175:CRIM1 protein, human; D015820:Cadherins; D008565:Membrane Proteins; D010750:Phosphoproteins; D034741:RNA, Small Interfering; D014157:Transcription Factors; D000091102:YAP-Signaling Proteins; C088374:YAP1 protein, human; D052004:Bone Morphogenetic Protein Receptors
keywords: CRIM1; YAP1; adhesion; cancer; migration