title: Agrobacterium-mediated transformation of tomato (Solanum lycopersicum L.) using the expansin 10 (CsEXP10) gene.
abstract: The cucumber expansin 10 (CsEXP10) gene was previously cloned from young cucumber fruits but its role has not been defined. To determine the role of this gene in plant growth and development, a CsEXP10 gene transformation system was established. The open reading frame of the gene was inserted behind the CaMV35S promoter of vector pCAMBIA1301, and the construct was introduced into tomato plants by Agrobacterium-mediated transformation. In total, 19 kanamycin-positive lines were produced and nine independent transgenic lines were identified by β-glucuronidase and polymerase chain reaction (PCR) analysis. Quantitative real-time PCR analysis showed that levels of the CsEXP10 transcript were higher in transgenic lines than in a non-transgenic line.
authors.affiliation: School of Horticulture and Landscape Architecture, Henan Institute of Science and Technology, Xinxiang, Henan, China sunyd201@163.com.
authors.forename: Y D
authors.initials: YD
authors.lastname: Sun
authors.affiliation: School of Horticulture and Landscape Architecture, Henan Institute of Science and Technology, Xinxiang, Henan, China.
authors.forename: W R
authors.initials: WR
authors.lastname: Luo
authors.affiliation: School of Horticulture and Landscape Architecture, Henan Institute of Science and Technology, Xinxiang, Henan, China.
authors.forename: S Y
authors.initials: SY
authors.lastname: Sun
authors.affiliation: School of Horticulture and Landscape Architecture, Henan Institute of Science and Technology, Xinxiang, Henan, China.
authors.forename: L
authors.initials: L
authors.lastname: Ni
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D023061:Gene Order; D005822:Genetic Vectors / Q000235:genetics; D018551:Solanum lycopersicum / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified; D014170:Transformation, Genetic
chemical_list: C446526:EXP10 protein, Lycopersicon esculentum; D010940:Plant Proteins