title: Inhibitory effect of polyozellin on secretory group IIA phospholipase A2.
abstract: The expression of secretory group IIA phospholipase A2 (sPLA2-IIA) is enhanced by development of inflammatory disorders. In this study, sPLA2-IIA expression was induced in the lipopolysaccharide (LPS)-stimulated human umbilical vein endothelial cells and mice to evaluate the effect of polyozellin. Polyozellin, a major constituent of a Korea edible mushroom Polyozellus multiplex, has been known to exhibit the biological activities such as anti-oxidative and anti-inflammatory effects. Polyozellin remarkably suppressed the LPS-mediated protein expression and activity of sPLA2-IIA via inhibition of phosphorylation of cytosolic phospholipase A2 and extracellular signal-regulated kinase 1/2. These results demonstrated that polyozellin might play an important role in the modulation of sPLA2-IIA expression and activity in response to the inflammatory diseases.
authors.affiliation: Department of Anatomy and Histology, College of Korean Medicine, Daegu Hanny University, Gyeongsan, 712-715, South Korea.
authors.forename: Sae-Kwang
authors.initials: SK
authors.lastname: Ku
authors.affiliation: College of Pharmacy, CMRI, Research Institute of Pharmaceutical Sciences, Kyungpook National University, 80 Daehak-ro, Buk-gu, Daegu, 702-701, Republic of Korea.
authors.forename: Eun-Ju
authors.initials: EJ
authors.lastname: Yang
authors.affiliation: Department of Anatomy and Histology, College of Korean Medicine, Daegu Hanny University, Gyeongsan, 712-715, South Korea.
authors.forename: Hyejin
authors.initials: H
authors.lastname: Kang
authors.affiliation: Department of Anatomy and Histology, College of Korean Medicine, Daegu Hanny University, Gyeongsan, 712-715, South Korea.
authors.forename: Byeongjin
authors.initials: B
authors.lastname: Jung
authors.affiliation: College of Pharmacy, CMRI, Research Institute of Pharmaceutical Sciences, Kyungpook National University, 80 Daehak-ro, Buk-gu, Daegu, 702-701, Republic of Korea. baejs@knu.ac.kr.
authors.forename: Jong-Sup
authors.initials: JS
authors.lastname: Bae
mesh_terms: D000363:Agaricales / Q000737:chemistry; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology*; D002478:Cells, Cultured; D051546:Cyclooxygenase 2 / Q000378:metabolism; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D005663:Furans / Q000302:isolation & purification / Q000494:pharmacology*; D054501:Group II Phospholipases A2 / Q000037:antagonists & inhibitors* / Q000378:metabolism; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects* / Q000201:enzymology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D008813:Mice, Inbred ICR; D010766:Phosphorylation; D000071876:Prostaglandin-E Synthases / Q000378:metabolism; D018805:Sepsis / Q000188:drug therapy* / Q000201:enzymology / Q000382:microbiology; D013997:Time Factors
chemical_list: D000893:Anti-Inflammatory Agents; D004791:Enzyme Inhibitors; D005663:Furans; D008070:Lipopolysaccharides; C109015:polyozellin; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; D048049:Extracellular Signal-Regulated MAP Kinases; D054501:Group II Phospholipases A2; C516287:PLA2G2A protein, human; C516288:Pla2g2a protein, mouse; C000605862:PTGES protein, human; D000071876:Prostaglandin-E Synthases
keywords: HUVECs; Inflammation; Polyozellin; sPLA2-IIA