title: In Vivo Imaging of Glycol Chitosan-Based Nanogel Biodistribution.
abstract: The preclinical development of nanomedicines raises several challenges and requires a comprehensive characterization. Among them is the evaluation of the biodistribution following systemic administration. In previous work, the biocompatibility and in vitro targeting ability of a glycol chitosan (GC) based nanogel have been validated. In the present study, its biodistribution in the mice is assessed, using near-infrared (NIR) fluorescence imaging as a tool to track the nanogel over time, after intravenous administration. Rapid whole body biodistribution of both Cy5.5 labeled GC nanogel and free polymer is found at early times. It remains widespreadly distributed in the body at least up to 6 h postinjection and its concentration then decreases drastically after 24 h. Nanogel blood circulation half-life lies around 2 h with the free linear GC polymer presenting lower blood clearance rate. After 24 h, the blood NIR fluorescence intensity associated with both samples decreases to insignificant values. NIR imaging of the organs shows that the nanogel had a body clearance time of ≈48 h, because at this time point a weak signal of NIR fluorescence is observed only in the kidneys. Hereupon it can be concluded that the engineered GC nanogel has a fairly long blood circulation time, suitable for biomedical applications, namely, drug delivery, simultaneously allowing efficient and quick body clearance.
authors.affiliation: Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057, Braga, Portugal.
authors.forename: Paula
authors.initials: P
authors.lastname: Pereira
authors.affiliation: I3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto and IBMC - Instituto de Biologia Molecular e Celular Rua do Campo Alegre, 4099-003, Porto, Portugal.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Correia
authors.affiliation: Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057, Braga, Portugal.
authors.forename: Francisco M
authors.initials: FM
authors.lastname: Gama
mesh_terms: D000818:Animals; D002232:Carbocyanines / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D048271:Chitosan / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D005782:Gels; D006018:Glycols / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D053758:Nanoparticles / Q000737:chemistry*; D061848:Optical Imaging / Q000379:methods*
chemical_list: C098793:CY5.5 cyanine dye; D002232:Carbocyanines; D005782:Gels; D006018:Glycols; D048271:Chitosan
keywords: Cy5.5; NIR imaging; biodistribution; blood clearance; glycol chitosan; nanogel