title: Collagen Promotes Higher Adhesion, Survival and Proliferation of Mesenchymal Stem Cells.
abstract: Mesenchymal stem cells (MSC) can differentiate into several cell types and are desirable candidates for cell therapy and tissue engineering. However, due to poor cell survival, proliferation and differentiation in the patient, the therapy outcomes have not been satisfactory. Although several studies have been done to understand the conditions that promote proliferation, differentiation and migration of MSC in vitro and in vivo, still there is no clear understanding on the effect of non-cellular bio molecules. Of the many factors that influence the cell behavior, the immediate cell microenvironment plays a major role. In this context, we studied the effect of extracellular matrix (ECM) proteins in controlling cell survival, proliferation, migration and directed MSC differentiation. We found that collagen promoted cell proliferation, cell survival under stress and promoted high cell adhesion to the cell culture surface. Increased osteogenic differentiation accompanied by high active RHOA (Ras homology gene family member A) levels was exhibited by MSC cultured on collagen. In conclusion, our study shows that collagen will be a suitable matrix for large scale production of MSC with high survival rate and to obtain high osteogenic differentiation for therapy.
authors.affiliation: Stem Cell Biology Lab, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, Assam, India.
authors.forename: Chinnapaka
authors.initials: C
authors.lastname: Somaiah
authors.affiliation: Stem Cell Biology Lab, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, Assam, India.
authors.forename: Atul
authors.initials: A
authors.lastname: Kumar
authors.affiliation: Stem Cell Biology Lab, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, Assam, India.
authors.forename: Darilang
authors.initials: D
authors.lastname: Mawrie
authors.affiliation: Stem Cell Biology Lab, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, Assam, India.
authors.forename: Amit
authors.initials: A
authors.lastname: Sharma
authors.affiliation: Stem Cell Biology Lab, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, Assam, India.
authors.forename: Suraj Dasharath
authors.initials: SD
authors.lastname: Patil
authors.affiliation: Department of Hematology, Gauhati Medical College Hospital, Assam, India.
authors.forename: Jina
authors.initials: J
authors.lastname: Bhattacharyya
authors.affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, Assam, India.
authors.forename: Rajaram
authors.initials: R
authors.lastname: Swaminathan
authors.affiliation: Stem Cell Biology Lab, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, Assam, India.
authors.forename: Bithiah Grace
authors.initials: BG
authors.lastname: Jaganathan
mesh_terms: D000199:Actins / Q000378:metabolism; D050156:Adipogenesis / Q000187:drug effects; D001854:Bone Marrow Cells / Q000166:cytology; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000737:chemistry / Q000494:pharmacology*; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D008856:Microscopy, Fluorescence; D008928:Mitochondria / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects; D012333:RNA, Messenger / Q000378:metabolism; D020742:rhoA GTP-Binding Protein / Q000235:genetics / Q000378:metabolism
chemical_list: D000199:Actins; D016326:Extracellular Matrix Proteins; D012333:RNA, Messenger; C066737:RHOA protein, human; D003094:Collagen; D020742:rhoA GTP-Binding Protein