title: Studies on the Coordination of Ribosomal Protein Assembly Events Involved in Processing and Stabilization of Yeast Early Large Ribosomal Subunit Precursors.
abstract: Cellular production of ribosomes involves the formation of highly defined interactions between ribosomal proteins (r-proteins) and ribosomal RNAs (rRNAs). Moreover in eukaryotic cells, efficient ribosome maturation requires the transient association of a large number of ribosome biogenesis factors (RBFs) with newly forming ribosomal subunits. Here, we investigated how r-protein assembly events in the large ribosomal subunit (LSU) rRNA domain II are coordinated with each other and with the association of RBFs in early LSU precursors of the yeast Saccharomyces cerevisiae. Specific effects on the pre-ribosomal association of RBFs could be observed in yeast mutants blocked in LSU rRNA domain II assembly. Moreover, formation of a cluster of r-proteins was identified as a downstream event in LSU rRNA domain II assembly. We analyzed in more detail the functional relevance of eukaryote specific bridges established by this r-protein cluster between LSU rRNA domain II and VI and discuss how they can support the stabilization and efficient processing of yeast early LSU precursor RNAs.
authors.affiliation: Lehrstuhl für Biochemie III, Universität Regensburg, Regensburg, Germany.
authors.forename: Uli
authors.initials: U
authors.lastname: Ohmayer
authors.affiliation: Instituto de Biología Funcional y Genómica (IBFG), CSIC/Universidad de Salamanca, Salamanca, Spain.
authors.forename: Álvaro
authors.initials: Á
authors.lastname: Gil-Hernández
authors.affiliation: Lehrstuhl für Biochemie III, Universität Regensburg, Regensburg, Germany.
authors.forename: Martina
authors.initials: M
authors.lastname: Sauert
authors.affiliation: Instituto de Biología Funcional y Genómica (IBFG), CSIC/Universidad de Salamanca, Salamanca, Spain.
authors.forename: Pilar
authors.initials: P
authors.lastname: Martín-Marcos
authors.affiliation: Instituto de Biología Funcional y Genómica (IBFG), CSIC/Universidad de Salamanca, Salamanca, Spain.
authors.forename: Mercedes
authors.initials: M
authors.lastname: Tamame
authors.affiliation: Lehrstuhl für Biochemie III, Universität Regensburg, Regensburg, Germany.
authors.forename: Herbert
authors.initials: H
authors.lastname: Tschochner
authors.affiliation: Lehrstuhl für Biochemie III, Universität Regensburg, Regensburg, Germany.
authors.forename: Joachim
authors.initials: J
authors.lastname: Griesenbeck
authors.affiliation: Lehrstuhl für Biochemie III, Universität Regensburg, Regensburg, Germany.
authors.forename: Philipp
authors.initials: P
authors.lastname: Milkereit
mesh_terms: D002352:Carrier Proteins / Q000378:metabolism; D008958:Models, Molecular; D009687:Nuclear Proteins / Q000378:metabolism; D011487:Protein Conformation; D054730:Protein Interaction Domains and Motifs; D011499:Protein Processing, Post-Translational; D055550:Protein Stability; D012323:RNA Processing, Post-Transcriptional; D012331:RNA, Fungal / Q000378:metabolism; D012335:RNA, Ribosomal / Q000378:metabolism; D016601:RNA-Binding Proteins; D012269:Ribosomal Proteins / Q000737:chemistry / Q000378:metabolism*; D054683:Ribosome Subunits, Large, Eukaryotic / Q000737:chemistry / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; C429647:NOC2 protein, S cerevisiae; D009687:Nuclear Proteins; D012331:RNA, Fungal; D012335:RNA, Ribosomal; D016601:RNA-Binding Proteins; D012269:Ribosomal Proteins; D029701:Saccharomyces cerevisiae Proteins; C027937:ribosomal protein L16