title: EMMPRIN expression is involved in the development of interstitial fibrosis and tubular atrophy in human kidney allografts.
abstract: BACKGROUND
  Matrix metalloproteinases (MMP) are involved in the development of interstitial fibrosis and tubular atrophy (IF/TA) in renal disease. The synthesis of MMP is activated by the extracellular matrix metalloproteinases inducer protein (EMMPRIN). To analyze the role of EMMPRIN in IF/TA, we retrospectively detected EMMPRIN expression in specimens of human renal allografts with various levels of IF/TA.
  
  
  METHODS
  Immunohistochemistry was performed to detect EMMPRIN expression. In a retrospective analysis, a total cohort of 50 specimens were divided according to BANFF-classification into four subgroups (0-3): no, mild (≤ 25%), moderate (26-50%), or severe (>50%) IF/TA. Among other parameters, renal function was analyzed and compared to EMMPRIN expression.
  
  
  RESULTS
  In 24 of 38 biopsies, we detected positive EMMPRIN staining. All nephrectomy (n = 12) samples were negative for EMMPRIN. Positive staining in the biopsy samples was detectable on the basolateral side of tubular epithelial cells. EMMPRIN staining was negatively correlated with IF/TA (p < 0.001). We found significant differences between the mean EMMPRIN expression in IF/TA groups 0 and 3 (p = 0.021) and groups 1 and 3 (p = 0.004). Furthermore, we found significant correlations between EMMPRIN staining and renal function.
  
  
  CONCLUSIONS
  Our data suggest that EMMPRIN is involved in the pathophysiology of IF/TA.
authors.affiliation: Department of Nephrology, Klinikum rechts der Isar, Technische Universität München, Munich, Germany.
authors.forename: Stephan
authors.initials: S
authors.lastname: Kemmner
authors.affiliation: Department of Nephrology, Klinikum rechts der Isar, Technische Universität München, Munich, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Schulte
authors.affiliation: Department of Pathology, Klinikum rechts der Isar, Technische Universität München, Munich, Germany.
authors.forename: Claus Hann
authors.initials: CH
authors.lastname: von Weyhern
authors.affiliation: Deutsches Herzzentrum München, Technische Universität München, Munich, Germany.
authors.forename: Roland
authors.initials: R
authors.lastname: Schmidt
authors.affiliation: Department of Nephrology, Klinikum rechts der Isar, Technische Universität München, Munich, Germany.
authors.forename: Marcus
authors.initials: M
authors.lastname: Baumann
authors.affiliation: Department of Nephrology, Klinikum rechts der Isar, Technische Universität München, Munich, Germany.
authors.forename: Uwe
authors.initials: U
authors.lastname: Heemann
authors.affiliation: Department of Nephrology, Klinikum rechts der Isar, Technische Universität München, Munich, Germany.
authors.forename: Lutz
authors.initials: L
authors.lastname: Renders
authors.affiliation: Department of Nephrology, Klinikum rechts der Isar, Technische Universität München, Munich, Germany.
authors.forename: Christoph
authors.initials: C
authors.lastname: Schmaderer
mesh_terms: D064591:Allografts; D001284:Atrophy / Q000209:etiology / Q000378:metabolism / Q000473:pathology*; D051926:Basigin / Q000378:metabolism*; D005260:Female; D005355:Fibrosis / Q000209:etiology / Q000378:metabolism / Q000473:pathology*; D005500:Follow-Up Studies; D006084:Graft Rejection / Q000175:diagnosis* / Q000378:metabolism*; D006801:Humans; D007677:Kidney Function Tests; D016030:Kidney Transplantation / Q000009:adverse effects*; D007684:Kidney Tubules / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009392:Nephrectomy / Q000009:adverse effects; D011183:Postoperative Complications; D011379:Prognosis; D012189:Retrospective Studies; D012307:Risk Factors
chemical_list: C497886:BSG protein, human; D051926:Basigin
keywords: CD147/basigin; chronic allograft dysfunction; extracellular matrix metalloproteinases inducer; interstitial fibrosis and tubular atrophy; kidney transplantation; matrix metalloproteinases