title: In Vitro Fermentation of Xylooligosaccharides Produced from Miscanthus × giganteus by Human Fecal Microbiota.
abstract: Purified xylooligosaccharides from Miscanthus × giganteus (M×G XOS) were used in an in vitro fermentation experiment inoculated with human fecal microbiota. A commercial XOS product and pectin were used as controls. Decreases in pH by 2.3, 2.4, and 2.0 units and production of short-chain fatty acids (SCFA; acetic acid, 7764.2, 6664.1, and 6387.9 μmol/g; propionic acid, 1006.7, 1089.5, and 661.5 μmol/g; and butyric acid, 955.5, 1252.9, and 917.7 μmol/g) were observed in M×G XOS, commercial XOS, and pectin medium after 12 h of fermentation, respectively. Titers of Bifidobacterium spp., Lactobacillus spp., and Escherichia coli increased when fed all three substrates as monitored by qPCR. There was no significant trend for Clostridium perfringens. During fermentation, M×G XOS was statistically equivalent in performance to the commercial XOS sample as measured by culture acidification and growth of health-promoting bacteria and resulted in the highest SCFA production among the three substrates.
authors.affiliation: Department of Agricultural and Biological Engineering, University of Illinois at Urbana-Champaign , 1304 West Pennsylvania Avenue, Urbana, Illinois 61801, United States.
authors.forename: Ming-Hsu
authors.initials: MH
authors.lastname: Chen
authors.affiliation: Department of Animal Sciences, University of Illinois at Urbana-Champaign , 1207 West Gregory Drive, Urbana, Illinois 61801, United States.
authors.forename: Kelly S
authors.initials: KS
authors.lastname: Swanson
authors.affiliation: Department of Animal Sciences, University of Illinois at Urbana-Champaign , 1207 West Gregory Drive, Urbana, Illinois 61801, United States.
authors.forename: George C
authors.initials: GC
authors.lastname: Fahey
authors.affiliation: Bioenergy Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture , 1815 North University Street, Peoria, Illinois 61604, United States.
authors.forename: Bruce S
authors.initials: BS
authors.lastname: Dien
authors.affiliation: Department of Animal Sciences, University of Illinois at Urbana-Champaign , 1207 West Gregory Drive, Urbana, Illinois 61801, United States.
authors.forename: Alison N
authors.initials: AN
authors.lastname: Beloshapka
authors.affiliation: Department of Animal Sciences, University of Illinois at Urbana-Champaign , 1207 West Gregory Drive, Urbana, Illinois 61801, United States.
authors.forename: Laura L
authors.initials: LL
authors.lastname: Bauer
authors.affiliation: Department of Agricultural and Biological Engineering, University of Illinois at Urbana-Champaign , 1304 West Pennsylvania Avenue, Urbana, Illinois 61801, United States.
authors.forename: Kent D
authors.initials: KD
authors.lastname: Rausch
authors.affiliation: Department of Agricultural and Biological Engineering, University of Illinois at Urbana-Champaign , 1304 West Pennsylvania Avenue, Urbana, Illinois 61801, United States.
authors.forename: M E
authors.initials: ME
authors.lastname: Tumbleson
authors.affiliation: Department of Agricultural and Biological Engineering, University of Illinois at Urbana-Champaign , 1304 West Pennsylvania Avenue, Urbana, Illinois 61801, United States.
authors.forename: Vijay
authors.initials: V
authors.lastname: Singh
mesh_terms: D001419:Bacteria / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism*; D005232:Fatty Acids, Volatile / Q000378:metabolism; D005243:Feces / Q000382:microbiology*; D005285:Fermentation; D005965:Glucuronates / Q000378:metabolism*; D006801:Humans; D064307:Microbiota; D009844:Oligosaccharides / Q000378:metabolism*; D006109:Poaceae / Q000378:metabolism / Q000382:microbiology*; D056692:Prebiotics / Q000032:analysis / Q000382:microbiology
chemical_list: D005232:Fatty Acids, Volatile; D005965:Glucuronates; D009844:Oligosaccharides; D056692:Prebiotics; C570991:xylooligosaccharide
keywords: Miscanthus × giganteus; biorefinery; coproducts; fecal microbiota; in vitro fermentation; prebiotics; xylooligosaccharides