title: Albumin adsorption on CoCrMo alloy surfaces.
abstract: Proteins can adsorb on the surface of artificial joints immediately after being implanted. Although research studying protein adsorption on medical material surfaces has been carried out, the mechanism of the proteins' adsorption which affects the corrosion behaviour of such materials still lacks in situ observation at the micro level. The adsorption of bovine serum albumin (BSA) on CoCrMo alloy surfaces was studied in situ by AFM and SKPFM as a function of pH and the charge of CoCrMo alloy surfaces. Results showed that when the specimens were uncharged, hydrophobic interaction could govern the process of the adsorption rather than electrostatic interaction, and BSA molecules tended to adsorb on the surfaces forming a monolayer in the side-on model. Results also showed that adsorbed BSA molecules could promote the corrosion process for CoCrMo alloys. When the surface was positively charged, the electrostatic interaction played a leading role in the adsorption process. The maximum adsorption occurred at the isoelectric point (pH 4.7) of BSA.
authors.affiliation: Corrosion and Protection Center, Key Laboratory for Environmental Fracture (MOE) University of Science and Technology Beijing, Beijing 100083, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Yan
authors.affiliation: Corrosion and Protection Center, Key Laboratory for Environmental Fracture (MOE) University of Science and Technology Beijing, Beijing 100083, China.
authors.forename: Hongjuan
authors.initials: H
authors.lastname: Yang
authors.affiliation: Corrosion and Protection Center, Key Laboratory for Environmental Fracture (MOE) University of Science and Technology Beijing, Beijing 100083, China.
authors.forename: Yanjing
authors.initials: Y
authors.lastname: Su
authors.affiliation: Corrosion and Protection Center, Key Laboratory for Environmental Fracture (MOE) University of Science and Technology Beijing, Beijing 100083, China.
authors.forename: Lijie
authors.initials: L
authors.lastname: Qiao
mesh_terms: D000327:Adsorption; D000818:Animals; D002417:Cattle; D002858:Chromium Alloys / Q000737:chemistry*; D003035:Cobalt / Q000737:chemistry*; D055664:Electrochemical Techniques / Q000379:methods; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D007526:Isoelectric Point; D018625:Microscopy, Atomic Force / Q000379:methods; D008982:Molybdenum / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D055672:Static Electricity
chemical_list: D002858:Chromium Alloys; D012710:Serum Albumin, Bovine; D003035:Cobalt; D008982:Molybdenum