title: Human IgG detection in serum on polymer based Mach-Zehnder interferometric biosensors.
abstract: We report a new method for detecting human IgG (hIgG) in serum on integrated-optical Mach-Zehnder interferometer biosensors realized in a high index contrast polymer material system. In the linear range of the sensor (5-200 nM) we observed excellent signal recoveries (95-110%) in buffer and serum samples, which indicate the absence of matrix effects. Signal enhancement was reached by using secondary anti-human IgG antibodies, which bind to immobilized target IgGs and allow detecting concentrations down to 100 pM. This polymer based optical sensor is fully compatible with cost-efficient mass production technologies, which makes it an attractive alternative to inorganic optical sensors. Graphical abstract of the hIgG measured on polymer based photonic sensors using a direct binding assay and a signal enhancement strategy with secondary antibodies.
authors.affiliation: AIT Austrian Institute of Technology GmbH, Health & Environment Department, Molecular Diagnostics, 1220, Vienna, Austria, www.ait.ac.at. eva.melnik@ait.ac.at.
authors.forename: Eva
authors.initials: E
authors.lastname: Melnik
authors.affiliation: AIT Austrian Institute of Technology GmbH, Health & Environment Department, Molecular Diagnostics, 1220, Vienna, Austria, www.ait.ac.at.
authors.forename: Roman
authors.initials: R
authors.lastname: Bruck
authors.affiliation: AIT Austrian Institute of Technology GmbH, Health & Environment Department, Molecular Diagnostics, 1220, Vienna, Austria, www.ait.ac.at.
authors.forename: Paul
authors.initials: P
authors.lastname: Müellner
authors.affiliation: Phadia Austria GmbH, Part of Thermo Fisher Scientific Immuno Diagnostics, 1220, Vienna, Austria.
authors.forename: Thomas
authors.initials: T
authors.lastname: Schlederer
authors.affiliation: AIT Austrian Institute of Technology GmbH, Health & Environment Department, Molecular Diagnostics, 1220, Vienna, Austria, www.ait.ac.at.
authors.forename: Rainer
authors.initials: R
authors.lastname: Hainberger
authors.affiliation: University of Tübingen, Institute of Pharmaceutical Sciences, Auf der Morgenstelle 8, Tübingen, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Lämmerhofer
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D001774:Blood Chemical Analysis / Q000379:methods*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D007368:Interferometry; D008027:Light; D057230:Limit of Detection; D011108:Polymers / Q000737:chemistry*
chemical_list: D007074:Immunoglobulin G; D011108:Polymers
keywords: Mach-Zehnder interferometer; biosensor; human IgG; human serum; injection molding; polyimide; polymer waveguide