title: Ru(II)-polypyridyl surface functionalised gold nanoparticles as DNA targeting supramolecular structures and luminescent cellular imaging agents.
abstract: The development of Ru(II) functionalized gold nanoparticles 1–3·AuNP is described. These systems were found to be mono-disperse with a hydrodynamic radius of ca. 15 nm in water but gave rise to the formation of higher order structures in buffered solution. The interaction of 1–3·AuNP with DNA was also studied by spectroscopic and microscopic methods and suggested the formation of large self-assembly structures in solution. The uptake of 1–3·AuNP by cancer cells was studied using both confocal fluorescence as well as transmission electron microscopy (TEM), with the aim of investigating their potential as tools for cellular biology. These systems displaying a non-toxic profile with favourable photophysical properties may have application across various biological fields including diagnostics and therapeutics.
authors.affiliation: School of Chemistry and Trinity Biomedical Sciences Institute, Trinity College Dublin, Dublin 2, Ireland. gunnlaut@tcd.ie martinm9@tcd.ie.
authors.forename: Miguel
authors.initials: M
authors.lastname: Martínez-Calvo
authors.affiliation: School of Biochemistry and Immunology and Trinity Biomedical Sciences Institute, Trinity College, Dublin 2, Ireland. clive.williams@tcd.ie.
authors.forename: Kim N
authors.initials: KN
authors.lastname: Orange
authors.affiliation: School of Chemistry and Trinity Biomedical Sciences Institute, Trinity College Dublin, Dublin 2, Ireland. gunnlaut@tcd.ie martinm9@tcd.ie and Department of Chemistry, Maynooth University, National University of Ireland, Maynooth, Ireland.
authors.forename: Robert B P
authors.initials: RB
authors.lastname: Elmes
authors.affiliation: School of Chemistry and Trinity Biomedical Sciences Institute, Trinity College Dublin, Dublin 2, Ireland. gunnlaut@tcd.ie martinm9@tcd.ie.
authors.forename: Bjørn
authors.initials: B
authors.lastname: la Cour Poulsen
authors.affiliation: School of Biochemistry and Immunology and Trinity Biomedical Sciences Institute, Trinity College, Dublin 2, Ireland. clive.williams@tcd.ie.
authors.forename: D Clive
authors.initials: DC
authors.lastname: Williams
authors.affiliation: School of Chemistry and Trinity Biomedical Sciences Institute, Trinity College Dublin, Dublin 2, Ireland. gunnlaut@tcd.ie martinm9@tcd.ie.
authors.forename: Thorfinnur
authors.initials: T
authors.lastname: Gunnlaugsson
mesh_terms: D004273:DNA, Neoplasm / Q000378:metabolism*; D006046:Gold / Q000737:chemistry / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D009369:Neoplasms / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D061848:Optical Imaging; D012428:Ruthenium / Q000737:chemistry / Q000494:pharmacology
chemical_list: D004273:DNA, Neoplasm; D006046:Gold; D012428:Ruthenium