title: Structural, compositional, mechanical characterization and biological assessment of bovine-derived hydroxyapatite coatings reinforced with MgF2 or MgO for implants functionalization.
abstract: Hydroxyapatite (HA) is a consecrated biomaterial for bone reconstruction. In the form of thin films deposited by pulsed laser technologies, it can be used to cover metallic implants aiming to increase biocompatibility and osseointegration rate. HA of animal origin (bovine, BHA) reinforced with MgF2 (2wt.%) or MgO (5wt.%) were used for deposition of thin coatings with improved adherence, biocompatibility and antimicrobial activity. For pulsed laser deposition experiments, a KrF* (λ=248nm, τFWHM≤25ns) excimer laser source was used. The deposited structures were characterized from a physical-chemical point of view by X-Ray Diffraction, Fourier Transform Infra-Red Spectroscopy, Scanning Electron Microscopy in top- and cross-view modes, Energy Dispersive X-Ray Spectroscopy and Pull-out adherence tests. The microbiological assay using the HEp-2 cell line revealed that all target materials and deposited thin films are non-cytotoxic. We conducted tests on three strains isolated from patients with dental implants failure, i.e. Microccocus sp., Enterobacter sp. and Candida albicans sp. The most significant anti-biofilm effect against Microcococcus sp. strain, at 72h, was obtained in the presence of BHA:MgO thin films. For Enterobacter sp. strain a superior antimicrobial activity at 72h was noticed, in respect with simple BHA or Ti control. The enhanced antimicrobial performances, correlated with good cytocompatibility and mechanical properties recommend these biomaterials as an alternative to synthetic HA for the fabrication of reliable implant coatings for dentistry and other applications.
authors.affiliation: National Institute for Lasers, Plasma and Radiation Physics, Magurele RO-077125, Romania.
authors.forename: Natalia
authors.initials: N
authors.lastname: Mihailescu
authors.affiliation: National Institute of Materials Physics, Magurele RO-077125, Romania.
authors.forename: G E
authors.initials: GE
authors.lastname: Stan
authors.affiliation: National Institute for Lasers, Plasma and Radiation Physics, Magurele RO-077125, Romania.
authors.forename: L
authors.initials: L
authors.lastname: Duta
authors.affiliation: Department of Microbiology, Faculty of Biology, Bucharest RO-060101, Romania.
authors.forename: Mariana Carmen
authors.initials: MC
authors.lastname: Chifiriuc
authors.affiliation: Stefan S. Nicolau Institute of Virology, 85 Mihai Bravu Avenue, Bucharest RO-030304, Romania.
authors.forename: Coralia
authors.initials: C
authors.lastname: Bleotu
authors.affiliation: National Institute for Lasers, Plasma and Radiation Physics, Magurele RO-077125, Romania.
authors.forename: M
authors.initials: M
authors.lastname: Sopronyi
authors.affiliation: National Institute for Lasers, Plasma and Radiation Physics, Magurele RO-077125, Romania.
authors.forename: C
authors.initials: C
authors.lastname: Luculescu
authors.affiliation: Department of Bioengineering, Faculty of Engineering, Marmara University, Goztepe, Istanbul TR-34722, Turkey; Advance Nanomaterials Research Laboratory, Marmara University, Goztepe, Istanbul TR-34722, Turkey.
authors.forename: F N
authors.initials: FN
authors.lastname: Oktar
authors.affiliation: National Institute for Lasers, Plasma and Radiation Physics, Magurele RO-077125, Romania. Electronic address: ion.mihailescu@inflpr.ro.
authors.forename: I N
authors.initials: IN
authors.lastname: Mihailescu
mesh_terms: D000818:Animals; D002417:Cattle; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D017886:Durapatite / Q000737:chemistry*; D005459:Fluorides / Q000737:chemistry*; D017616:Magnesium Compounds / Q000737:chemistry*; D008277:Magnesium Oxide / Q000737:chemistry*; D019736:Prostheses and Implants
chemical_list: D020099:Coated Materials, Biocompatible; D017616:Magnesium Compounds; D008277:Magnesium Oxide; C031288:magnesium fluoride; D017886:Durapatite; D005459:Fluorides
keywords: Bovine bone; Highly adherent coatings; Pulsed laser deposition; Reinforced hydroxyapatite; Titanium