title: Protein disorder reduced in Saccharomyces cerevisiae to survive heat shock.
abstract: Recent experiments established that a culture of Saccharomyces cerevisiae (baker's yeast) survives sudden high temperatures by specifically duplicating the entire chromosome III and two chromosomal fragments (from IV and XII). Heat shock proteins (HSPs) are not significantly over-abundant in the duplication. In contrast, we suggest a simple algorithm to " postdict " the experimental results: Find a small enough chromosome with minimal protein disorder and duplicate this region. This algorithm largely explains all observed duplications. In particular, all regions duplicated in the experiment reduced the overall content of protein disorder. The differential analysis of the functional makeup of the duplication remained inconclusive. Gene Ontology (GO) enrichment suggested over-representation in processes related to reproduction and nutrient uptake. Analyzing the protein-protein interaction network (PPI) revealed that few network-central proteins were duplicated. The predictive hypothesis hinges upon the concept of reducing proteins with long regions of disorder in order to become less sensitive to heat shock attack.
authors.affiliation: Department of Informatics, Bioinformatics & Computational Biology, TUM, Munich, Germany ; Institute of Experimental Physics, Division of Biophysics, University of Warsaw, Warsaw, Poland.
authors.forename: Esmeralda
authors.initials: E
authors.lastname: Vicedo
authors.affiliation: Department of Informatics, Bioinformatics & Computational Biology, TUM, Munich, Germany ; Graduate School of Information Science in Health, TUM, Munich, Germany.
authors.forename: Zofia
authors.initials: Z
authors.lastname: Gasik
authors.affiliation: Department of Informatics, Bioinformatics & Computational Biology, TUM, Munich, Germany ; Institute of Systems Biology, Shanghai University, Shanghai, China.
authors.forename: Yu-An
authors.initials: YA
authors.lastname: Dong
authors.affiliation: Department of Informatics, Bioinformatics & Computational Biology, TUM, Munich, Germany.
authors.forename: Tatyana
authors.initials: T
authors.lastname: Goldberg
authors.affiliation: Department of Informatics, Bioinformatics & Computational Biology, TUM, Munich, Germany ; Institute of Advanced Study, TUM, Munich, Germany ; Institute for Food and Plant Sciences WZW, TUM, Freising, Germany.
authors.forename: Burkhard
authors.initials: B
authors.lastname: Rost
keywords: GO terms; Saccharomyces cerevisiae; chromosomal duplication; heat shock proteins; heat-shock stress; protein disorder; protein-protein interactions