title: Evaluation of the Nature of Collagen Fibers in KCOT, Dentigerous Cyst and Ameloblastoma using Picrosirius Red Stain - A Comparative Study.
abstract: BACKGROUND
  Reciprocal interaction between dental epithelium and mesenchyme is thought to be crucial for normal odontogenesis. Thus, the mesenchymal influence of the fibrous capsules may play an important role in the maintenance of epithelial expression. Collagen is the major component of the extracellular matrix and possibly there is an alteration in the nature and structure of collagen in various pathological conditions. Studies by polarizing microscopy have also shown that there is a difference in collagen and probably these differences may play a role in their biologic behaviour.
  
  
  OBJECTIVE
  The purpose of this study was to evaluate the nature of collagen fibers in keratocystic odontogenic tumour (KCOT), dentigerous cyst (DC), unicystic ameloblastoma (UA) and solid/multicystic ameloblastoma (SMA) and correlating this with their biological behaviour.
  
  
  METHODS
  Five diagnosed cases each of UA, SMA, KCOT and DC were taken and stained using Picrosirius red stain kit and evaluated using a polarizing microscope.
  
  
  METHODS
  Chi-square test was used to analyse the results.
  
  
  CONCLUSIONS
  Collagen fibers in dentigerous cysts showed predominant yellowish-red birefringence and fibers in KCOT and ameloblastomas showed a predominantly greenish-yellow birefringence. Hence, our study suggests that the nature and character of collagen fibers may influence the clinical behaviour of the lesion. Since ours is a pilot study, to corroborate our view, studies with larger sample size are required to substantiate the results.
authors.affiliation: Post Graduate Student, Department of Oral and Maxillofacial Pathology, SVS Institute of Dental Sciences , Mahabubnagar, Telangana, India .
authors.forename: Yukti
authors.initials: Y
authors.lastname: Raj
authors.affiliation: Professor and Head, Department of Oral and Maxillofacial Pathology, SVS Institute of Dental Sciences , Mahabubnagar, Telangana, India .
authors.forename: Manne Srinivas Muni
authors.initials: MS
authors.lastname: Sekhar
authors.affiliation: Professor, Department of Oral and Maxillofacial Pathology, SVS Institute of Dental Sciences , Mahabubnagar, Telangana, India .
authors.forename: Sanjeevareddygari
authors.initials: S
authors.lastname: Shylaja
authors.affiliation: Reader, Department of Oral and Maxillofacial Pathology, SVS Institute of Dental Sciences , Mahabubnagar, Telangana, India .
authors.forename: Sangala Nagendra
authors.initials: SN
authors.lastname: Bhavani
authors.affiliation: Senior Lecturer, Department of Oral and Maxillofacial Pathology, SVS Institute Of Dental Sciences , Mahabubnagar, Telangana, India .
authors.forename: Oruganti Venkata
authors.initials: OV
authors.lastname: Ramanand
authors.affiliation: Post Graduate Student, Department of Oral and Maxillofacial Pathology, SVS Institute of Dental Sciences , Mahabubnagar, Telangana, India .
authors.forename: Spandana
authors.initials: S
authors.lastname: Patha
authors.affiliation: Post Graduate Student, Department of Oral and Maxillofacial Pathology, SVS Institute of Dental Sciences , Mahabubnagar, Telangana, India .
authors.forename: Sharath Kumar
authors.initials: SK
authors.lastname: Reddy
authors.affiliation: Post Graduate Student, Department of Oral and Maxillofacial Pathology, SVS Institute of Dental Sciences , Mahabubnagar, Telangana, India .
authors.forename: Akula Sandhya
authors.initials: AS
authors.lastname: Rani
keywords: Collagen fibers; Odontogenic cysts and tumours; Picrosirius red; Polarizing microscopy