title: Resistance Mechanisms and Molecular Docking Studies of Four Novel QoI Fungicides in Peronophythora litchii.
abstract: Peronophythora litchii is the causal agent of litchi downy blight. Enestroburin, SYP-1620, SYP-2815 and ZJ0712 are four novel QoI fungicides developed by China. Eight mutants of P. litchii resistant to these QoI fungicides and azoxystrobin (as a known QoI fungicide) were obtained in our preliminary work. In this study, the full length of the cytochrome b gene in P. litchii, which has a full length of 382 amino acids, was cloned from both sensitive isolates and resistant mutants, and single-site mutations G142A, G142S, Y131C, or F128S were found in resistant mutants. Molecular docking was used to predict how the mutations alter the binding of the five QoI fungicides to the Qo-binding pockets. The results have increased our understanding of QoI fungicide-resistance mechanisms and may help in the development of more potent inhibitors against plant diseases in the fields.
authors.affiliation: Department of Plant Pathology, College of Agriculture and Biotechnology, China Agricultural University, Beijing, 100193, China.
authors.forename: Yuxin
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: College of Forestry, Beijing Forestry University, Beijing, 100083, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Chen
authors.affiliation: College of Agro-grassland Science, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Hu
authors.affiliation: Department of Chemistry, College of Science, China Agricultural University, Beijing, 100193, China.
authors.forename: Hongxia
authors.initials: H
authors.lastname: Duan
authors.affiliation: Department of Plant Pathology, College of Agriculture and Biotechnology, China Agricultural University, Beijing, 100193, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Lin
authors.affiliation: Department of Plant Pathology, College of Agriculture and Biotechnology, China Agricultural University, Beijing, 100193, China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Liu
authors.affiliation: Department of Plant Pathology, College of Agriculture and Biotechnology, China Agricultural University, Beijing, 100193, China.
authors.forename: Qingxiao
authors.initials: Q
authors.lastname: Meng
authors.affiliation: State Key Laboratory of the Discovery and Development of Novel Pesticide, Shenyang Research Institute of Chemical Industry co., Ltd., Shenyang, 110021, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.affiliation: State Key Laboratory of the Discovery and Development of Novel Pesticide, Shenyang Research Institute of Chemical Industry co., Ltd., Shenyang, 110021, China.
authors.forename: Naiguo
authors.initials: N
authors.lastname: Si
authors.affiliation: State Key Laboratory of the Discovery and Development of Novel Pesticide, Shenyang Research Institute of Chemical Industry co., Ltd., Shenyang, 110021, China.
authors.forename: Changling
authors.initials: C
authors.lastname: Liu
authors.affiliation: Department of Plant Pathology, College of Agriculture and Biotechnology, China Agricultural University, Beijing, 100193, China.
authors.forename: Xili
authors.initials: X
authors.lastname: Liu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002417:Cattle; D045303:Cytochromes b / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D025141:Drug Resistance, Fungal / Q000235:genetics; D005659:Fungicides, Industrial / Q000737:chemistry* / Q000494:pharmacology*; D008968:Molecular Conformation; D062105:Molecular Docking Simulation; D056004:Molecular Dynamics Simulation; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009154:Mutation; D009868:Oomycetes / Q000737:chemistry*; D040081:Protein Array Analysis; D016415:Sequence Alignment; D013329:Structure-Activity Relationship
chemical_list: D005659:Fungicides, Industrial; D045303:Cytochromes b