title: Quantification of Docetaxel in Serum Using Turbulent Flow Liquid Chromatography Electrospray Tandem Mass Spectrometry (TFC-HPLC-ESI-MS/MS).
abstract: Docetaxel is a second-generation taxane and is used clinically as an anti-neoplastic agent in cancer chemotherapy via an anti-mitotic mechanism. Its efficacy is limited to a narrow therapeutic window. Inappropriately high concentrations may cause erythema, fluid retention, nausea, diarrhea, and neutropenia. As a result, dosing recommendations have changed from high dosage loading every 3 weeks to lower dosage loading weekly. We describe a method that can be used for therapeutic drug monitoring of docetaxel levels using turbulent flow liquid chromatography electrospray tandem mass spectrometry (TFC-HPLC-ESI-MS/MS). The method is rapid, requiring only 6.3 min per analytical run following a simple protein crash. The method requires only 100 μL of serum. Concentrations of docetaxel were quantified by a calibration curve relating the peak-area ratio of docetaxel to a deuterated internal standard (docetaxel-D9). The method was linear from 7.8 to 1000 ng/mL, with imprecision ≤6.2 %.
authors.affiliation: Department of Pathology, The Johns Hopkins University School of Medicine, Meyer B-125, 600N. Wolfe Street, Baltimore, MD, 21287, USA.
authors.forename: Christopher A
authors.initials: CA
authors.lastname: Crutchfield
authors.affiliation: Department of Pathology, The Johns Hopkins University School of Medicine, Meyer B-125, 600N. Wolfe Street, Baltimore, MD, 21287, USA.
authors.forename: Mark A
authors.initials: MA
authors.lastname: Marzinke
authors.affiliation: Department of Pathology, The Johns Hopkins University School of Medicine, Meyer B-125, 600N. Wolfe Street, Baltimore, MD, 21287, USA. wclarke@jhmi.edu.
authors.forename: William A
authors.initials: WA
authors.lastname: Clarke
mesh_terms: D000970:Antineoplastic Agents / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D000077143:Docetaxel; D016903:Drug Monitoring / Q000379:methods*; D006801:Humans; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*; D043823:Taxoids / Q000097:blood*
chemical_list: D000970:Antineoplastic Agents; D043823:Taxoids; D000077143:Docetaxel
keywords: Anti-neoplastic; Docetaxel; Turbulent flow liquid chromatography electrospray tandem mass spectrometry