title: Arabidopsis Cell Division Cycle 20.1 Is Required for Normal Meiotic Spindle Assembly and Chromosome Segregation.
abstract: Cell division requires proper spindle assembly; a surveillance pathway, the spindle assembly checkpoint (SAC), monitors whether the spindle is normal and correctly attached to kinetochores. The SAC proteins regulate mitotic chromosome segregation by affecting CDC20 (Cell Division Cycle 20) function. However, it is unclear whether CDC20 regulates meiotic spindle assembly and proper homolog segregation. Here, we show that the Arabidopsis thaliana CDC20.1 gene is indispensable for meiosis and male fertility. We demonstrate that cdc20.1 meiotic chromosomes align asynchronously and segregate unequally and the metaphase I spindle has aberrant morphology. Comparison of the distribution of meiotic stages at different time points between the wild type and cdc20.1 reveals a delay of meiotic progression from diakinesis to anaphase I. Furthermore, cdc20.1 meiocytes exhibit an abnormal distribution of a histone H3 phosphorylation mark mediated by the Aurora kinase, providing evidence that CDC20.1 regulates Aurora localization for meiotic chromosome segregation. Further evidence that CDC20.1 and Aurora are functionally related was provided by meiosis-specific knockdown of At-Aurora1 expression, resulting in meiotic chromosome segregation defects similar to those of cdc20.1. Taken together, these results suggest a critical role for CDC20.1 in SAC-dependent meiotic chromosome segregation.
authors.affiliation: State Key Laboratory of Genetic Engineering and Collaborative Innovation Center of Genetics and Development, Ministry of Education Key Laboratory of Biodiversity Sciences and Ecological Engineering, Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai 200438, China.
authors.forename: Baixiao
authors.initials: B
authors.lastname: Niu
authors.affiliation: State Key Laboratory of Genetic Engineering and Collaborative Innovation Center of Genetics and Development, Ministry of Education Key Laboratory of Biodiversity Sciences and Ecological Engineering, Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai 200438, China.
authors.forename: Liudan
authors.initials: L
authors.lastname: Wang
authors.affiliation: Center for Genomics and Biotechnology, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
authors.forename: Liangsheng
authors.identifier: http://orcid.org/0000-0003-1919-3677
authors.initials: L
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Genetic Engineering and Collaborative Innovation Center of Genetics and Development, Ministry of Education Key Laboratory of Biodiversity Sciences and Ecological Engineering, Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai 200438, China.
authors.forename: Ding
authors.initials: D
authors.lastname: Ren
authors.affiliation: State Key Laboratory of Genetic Engineering and Collaborative Innovation Center of Genetics and Development, Ministry of Education Key Laboratory of Biodiversity Sciences and Ecological Engineering, Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai 200438, China.
authors.forename: Ren
authors.initials: R
authors.lastname: Ren
authors.affiliation: Department of Biology and the Carolina Center for Genome Sciences, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599-3280 Lineberger Comprehensive Cancer Center, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599-3280.
authors.forename: Gregory P
authors.identifier: http://orcid.org/0000-0002-7962-3862
authors.initials: GP
authors.lastname: Copenhaver
authors.affiliation: State Key Laboratory of Genetic Engineering and Collaborative Innovation Center of Genetics and Development, Ministry of Education Key Laboratory of Biodiversity Sciences and Ecological Engineering, Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai 200438, China Center for Evolutionary Biology, Institutes of Biomedical Sciences School of Life Sciences, Fudan University, Shanghai 200433, China yx_wang@fudan.edu.cn hongma@fudan.edu.cn.
authors.forename: Hong
authors.identifier: http://orcid.org/0000-0001-8717-4422
authors.initials: H
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Genetic Engineering and Collaborative Innovation Center of Genetics and Development, Ministry of Education Key Laboratory of Biodiversity Sciences and Ecological Engineering, Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai 200438, China yx_wang@fudan.edu.cn hongma@fudan.edu.cn.
authors.forename: Yingxiang
authors.identifier: http://orcid.org/0000-0001-6085-5615
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000705:Anaphase; D064173:Anaphase-Promoting Complex-Cyclosome / Q000235:genetics / Q000378:metabolism; D017360:Arabidopsis / Q000166:cytology / Q000235:genetics* / Q000502:physiology; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D064199:Cdc20 Proteins / Q000235:genetics* / Q000378:metabolism; D059447:Cell Cycle Checkpoints; D020090:Chromosome Segregation; D055785:Gene Knockdown Techniques; D018386:Kinetochores / Q000378:metabolism; D008540:Meiosis; D008677:Metaphase; D051479:Plant Infertility; D008941:Spindle Apparatus / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; C577480:CDC20 protein, Arabidopsis; D064199:Cdc20 Proteins; D064173:Anaphase-Promoting Complex-Cyclosome