title: Synthesis of rosmarinic acid analogues in Escherichia coli.
abstract: OBJECTIVE
  To produce rosmarinic acid analogues in the recombinant Escherichia coli BLRA1, harboring a 4-coumarate: CoA ligase from Arabidopsis thaliana (At4CL) and a rosmarinic acid synthase from Coleus blumei (CbRAS).
  
  
  RESULTS
  Incubation of the recombinant E. coli strain BLRA1 with exogenously supplied phenyllactic acid (PL) and analogues as acceptor substrates, and coumaric acid and analogues as donor substrates led to production of 18 compounds, including 13 unnatural RA analogues.
  
  
  CONCLUSIONS
  This work demonstrates the viability of synthesizing a broad range of rosmarinic acid analogues in E. coli, and sheds new light on the substrate specificity of CbRAS.
authors.affiliation: Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China.|Key Laboratory of Systems Microbial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China.
authors.forename: Yibin
authors.initials: Y
authors.lastname: Zhuang
authors.affiliation: Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China.|Department of Food Science and Technology, School of Biological Engineering, East China University of Science and Technology, Shanghai, 200237, China.
authors.forename: Jingjie
authors.initials: J
authors.lastname: Jiang
authors.affiliation: Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China.|Key Laboratory of Systems Microbial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China.
authors.forename: Huiping
authors.initials: H
authors.lastname: Bi
authors.affiliation: Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China.|Key Laboratory of Systems Microbial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Yin
authors.affiliation: Department of Food Science and Technology, School of Biological Engineering, East China University of Science and Technology, Shanghai, 200237, China. swliu@ecust.edu.cn.
authors.forename: Shaowei
authors.initials: S
authors.lastname: Liu
authors.affiliation: Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China. liu_t@tib.cas.cn.|Key Laboratory of Systems Microbial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China. liu_t@tib.cas.cn.
authors.forename: Tao
authors.initials: T
authors.lastname: Liu
mesh_terms: D002934:Cinnamates / Q000378:metabolism*; D003066:Coenzyme A Ligases / Q000235:genetics / Q000378:metabolism; D053630:Depsides; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics / Q000254:growth & development*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D060847:Metabolic Engineering / Q000379:methods; D013379:Substrate Specificity; D000097123:Rosmarinic Acid
chemical_list: D002934:Cinnamates; D003066:Coenzyme A Ligases; D053630:Depsides; D029968:Escherichia coli Proteins; D000097123:Rosmarinic Acid
keywords: 4-Coumarate:CoA ligase; Biosynthesis; Escherichia coli; Phenyllactic acid; Rosmarinic acid analogues; Rosmarinic acid synthase