title: Using Live-Cell Markers in Maize to Analyze Cell Division Orientation and Timing.
abstract: Recently developed live-cell markers provide an opportunity to explore the dynamics and localization of proteins in maize, an important crop and model for monocot development. A step-by-step method is outlined for observing and analyzing the process of division in maize cells. The steps include plant growth conditions, sample preparation, time-lapse setup, and calculation of division rates.
authors.affiliation: Department of Botany and Plant Sciences, Center for Plant Cell Biology, University of California, Riverside, 900 University Ave., Riverside, CA, 92521, USA. carolyn.rasmussen@ucr.edu.
authors.forename: Carolyn G
authors.initials: CG
authors.lastname: Rasmussen
mesh_terms: D002455:Cell Division; D002470:Cell Survival; D008164:Luminescent Proteins / Q000032:analysis; D018613:Microscopy, Confocal / Q000379:methods*; D008870:Microtubules / Q000648:ultrastructure; D008938:Mitosis; D003313:Zea mays / Q000166:cytology* / Q000254:growth & development / Q000648:ultrastructure
chemical_list: D008164:Luminescent Proteins
keywords: Cell division; Confocal; Fluorescent proteins; Live-cell imaging; Maize; Microscopy; Microtubules; Mitosis; Plant