title: [Specific PCR Molecular Identification of Euconunmia Cortex].
abstract: OBJECTIVE
  To screen specific DNA sequence by rDNA-ITS sequence analysis at Eucommmia Cortex and its adulterants, then specific primers were designed to identify Eucommmia Cortex rapidly.
  
  
  METHODS
  rDNA-ITS sequences of Eucommmia Cortex and its adulterants were downloaded from NCBI. The specific DNA sequence in the rDNA-ITS sequence of Eucommmia Cortex was found by compared with all sequences. Specific primers for authentic the Eucommmia Cortex were designed. PCR amplification and detection methods were optimized.
  
  
  RESULTS
  PCR amplification products were detected through agarose gel electrophoresis and fluorescence reaction. The 400 bp identification band for Eucommmia Cortex could be produced and green fluorescent could be observed under ultraviolet light after adding the dye SYBR Green I. However its adulterants were without identification hand and green fluorescent.
  
  
  CONCLUSIONS
  Specific PCR molecular identification can be applied to identify Eucommmia Cortex, the time is shortened significantly by PCR amplification optimization, and fluorescence detection can be more visually display the identification results. In a word, this study provides new ideas and methods for rapid identification of Eucommmia Cortex.
authors.forename: Ling
authors.initials: L
authors.lastname: Ding
authors.forename: Dan
authors.initials: D
authors.lastname: Zhao
authors.forename: Tao
authors.initials: T
authors.lastname: Zhou
authors.forename: Wei-ke
authors.initials: WK
authors.lastname: Jiang
authors.forename: Cheng-hong
authors.initials: CH
authors.lastname: Xiao
mesh_terms: D004396:Coloring Agents; D017931:DNA Primers; D018744:DNA, Plant / Q000235:genetics; D004365:Drugs, Chinese Herbal; D004587:Electrophoresis, Agar Gel; D031283:Eucommiaceae / Q000235:genetics*; D005453:Fluorescence; D010880:Piperidines; D024301:Plant Bark; D016133:Polymerase Chain Reaction; D013892:Thioxanthenes
chemical_list: D004396:Coloring Agents; D017931:DNA Primers; D018744:DNA, Plant; D004365:Drugs, Chinese Herbal; D010880:Piperidines; D013892:Thioxanthenes; C004327:pimethixene