title: BrdU-label-retaining cells in rat eccrine sweat glands over time.
abstract: Cell proliferation and turnover are fueled by stem cells. In a previous study, we demonstrated that rat eccrine sweat glands contained abundant bromodeoxyuridine (BrdU)-label-retaining cells (LRCs). However, morphological observations showed that eccrine sweat glands usually show little or no signs of homeostatic change. In this study, we account for why the homeostatic change is rare in eccrine sweat glands based on cytokinetic changes in BrdU-LRC turnover, and also determine the BrdU-labeled cell type. Thirty-six newborn SD rats, were injected intraperitoneally with 50mg/kg BrdU twice daily at a 2h interval for 4 consecutive days. After a chase period of 4, 6, 8, 12, 24 and 32 weeks, rats were euthanized, and the hind footpads were removed and processed for BrdU immunostaining, and BrdU/α-SMA and BrdU/K14 double-immunostaining. BrdU-LRCs were observed in the ducts, secretory coils and mesenchymal cells at all survival time points. The percentage of BrdU(+) cells in rat eccrine sweat glands averaged 4.2±1.2% after 4 weeks of chase, increased slightly by the 6th week, averaging 4.4±0.9%, and peaked at 8 weeks, averaging 5.3±1.0%. Subsequently, the average percentage of BrdU(+) cells declined to 3.2±0.8% by the 32nd week. There was no difference in the percentage of BrdU-LRCs among the different survival time points except that a significant difference in the percentage of BrdU-LRCs detected at 24 weeks versus 8 weeks, and 32 weeks versus 8 weeks, was observed. We concluded that the BrdU-LRCs turnover is slow in eccrine sweat glands.
authors.affiliation: Burn and Plastic Surgery, The Second Affiliated Hospital, Shantou University Medical College, North Dongxia Road, Shantou 515041, Guangdong Province, China; Research Center for Translational Medicine, Shantou University Medical College, North Dongxia Road, Shantou 515041, Guangdong Province, China. Electronic address: lihaihong1051@126.com.
authors.forename: Haihong
authors.initials: H
authors.lastname: Li
authors.affiliation: Burn and Plastic Surgery, The Second Affiliated Hospital, Shantou University Medical College, North Dongxia Road, Shantou 515041, Guangdong Province, China.
authors.forename: Mingjun
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Burn and Plastic Surgery, The Second Affiliated Hospital, Shantou University Medical College, North Dongxia Road, Shantou 515041, Guangdong Province, China.
authors.forename: Xuexue
authors.initials: X
authors.lastname: Li
authors.affiliation: Burn and Plastic Surgery, The Second Affiliated Hospital, Shantou University Medical College, North Dongxia Road, Shantou 515041, Guangdong Province, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Chen
authors.affiliation: Research Center for Translational Medicine, Shantou University Medical College, North Dongxia Road, Shantou 515041, Guangdong Province, China.
authors.forename: Bingna
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Burn and Plastic Surgery, The Second Affiliated Hospital, Shantou University Medical College, North Dongxia Road, Shantou 515041, Guangdong Province, China.
authors.forename: Shijie
authors.initials: S
authors.lastname: Tang
authors.affiliation: Burns Institute, The First Affiliated Hospital, Chinese PLA General Hospital, Trauma Center of Postgraduate Medical School, 51 Fucheng Road, Beijing 100037, China.
authors.forename: Xiaobing
authors.initials: X
authors.lastname: Fu
mesh_terms: D000818:Animals; D001973:Bromodeoxyuridine / Q000378:metabolism; D049109:Cell Proliferation; D004439:Eccrine Glands / Q000166:cytology* / Q000378:metabolism; D005260:Female; D007150:Immunohistochemistry; D007202:Indicators and Reagents / Q000378:metabolism; D008297:Male; D017207:Rats, Sprague-Dawley
chemical_list: D007202:Indicators and Reagents; D001973:Bromodeoxyuridine
keywords: Cell turnover; Eccrine sweat glands; Label-retaining cells (LRCs); Rat; Stem cell