title: Complete genome sequence of and proposal of Thermofilum uzonense sp. nov. a novel hyperthermophilic crenarchaeon and emended description of the genus Thermofilum.
abstract: A strain of a hyperthermophilic filamentous archaeon was isolated from a sample of Kamchatka hot spring sediment. Isolate 1807-2 grew optimally at 85 °C, pH 6.0-6.5, the parameters being close to those at the sampling site. 16S rRNA gene sequence analysis placed the novel isolate in the crenarchaeal genus Thermofilum; Thermofilum pendens was its closest valid relative (95.7 % of sequence identity). Strain 1807-2 grew organothrophically using polysaccharides (starch and glucomannan), yeast extract or peptone as substrates. The addition of other crenarchaea culture broth filtrates was obligatory required for growth and could not be replaced by the addition of these organisms' cell wall fractions, as it was described for T. pendens. The genome of strain 1807-2 was sequenced using Illumina and PGM technologies. The average nucleotide identities between genome of strain 1807-2 and T. pendens strain HRK 5(T) and "T. adornatus" strain 1910b were 85 and 82 %, respectively. On the basis of 16S rRNA gene sequence phylogeny, ANI calculations and phenotypic differences we propose a novel species Thermofilum uzonense with the type strain 1807-2(T) (= DSM 28062(T) = JCM 19810(T)). Project information and genome sequence was deposited in Genbank under IDs PRJNA262459 and CP009961, respectively.
authors.affiliation: Immanuel Kant Baltic Federal University, Kaliningrad, Russian Federation.
authors.forename: Stepan V
authors.initials: SV
authors.lastname: Toshchakov
authors.affiliation: Immanuel Kant Baltic Federal University, Kaliningrad, Russian Federation.
authors.forename: Aleksei A
authors.initials: AA
authors.lastname: Korzhenkov
authors.affiliation: Immanuel Kant Baltic Federal University, Kaliningrad, Russian Federation.
authors.forename: Nazar I
authors.initials: NI
authors.lastname: Samarov
authors.affiliation: Immanuel Kant Baltic Federal University, Kaliningrad, Russian Federation.
authors.forename: Ilia O
authors.initials: IO
authors.lastname: Mazunin
authors.affiliation: Immanuel Kant Baltic Federal University, Kaliningrad, Russian Federation.
authors.forename: Oleg I
authors.initials: OI
authors.lastname: Mozhey
authors.affiliation: Pirogov Russian National Research Medical University, Moscow, Russian Federation.
authors.forename: Ilya S
authors.initials: IS
authors.lastname: Shmyr
authors.affiliation: Winogradsky Institute of Microbiology, Research Center for Biotechnology Russian Academy of Sciences, Moscow, Russian Federation.
authors.forename: Ksenia S
authors.initials: KS
authors.lastname: Derbikova
authors.affiliation: Winogradsky Institute of Microbiology, Research Center for Biotechnology Russian Academy of Sciences, Moscow, Russian Federation.
authors.forename: Evgeny A
authors.initials: EA
authors.lastname: Taranov
authors.affiliation: Immanuel Kant Baltic Federal University, Kaliningrad, Russian Federation.
authors.forename: Irina N
authors.initials: IN
authors.lastname: Dominova
authors.affiliation: Winogradsky Institute of Microbiology, Research Center for Biotechnology Russian Academy of Sciences, Moscow, Russian Federation.
authors.forename: Elizaveta A
authors.initials: EA
authors.lastname: Bonch-Osmolovskaya
authors.affiliation: Immanuel Kant Baltic Federal University, Kaliningrad, Russian Federation.
authors.forename: Maxim V
authors.initials: MV
authors.lastname: Patrushev
authors.affiliation: Winogradsky Institute of Microbiology, Research Center for Biotechnology Russian Academy of Sciences, Moscow, Russian Federation.
authors.forename: Olga A
authors.initials: OA
authors.lastname: Podosokorskaya
authors.affiliation: Winogradsky Institute of Microbiology, Research Center for Biotechnology Russian Academy of Sciences, Moscow, Russian Federation.
authors.forename: Ilya V
authors.initials: IV
authors.lastname: Kublanov
keywords: Crenarchaeota; Kamchatka; Phylogeny; Polysaccharides hydrolysis; Thermofilum; hyperthermophile