title: [Effect of Electroacupuncture Intervention at Different Time-points on Levels of HSP 70, MDA, SOD and GSH-PX of Liver in Rats with Simulated Weightlessness].
abstract: OBJECTIVE
  To observe the effect of acupuncture intervention at different time-points on the activity of superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), the content of malonaldehyde (MDA) and expression of heat shock protein 70 (HSP 70) of liver tissue in rats with simulated weightlessness, so as to explore its mechanism underlying improvement of liver injury in rats with simulated weightlessness.
  
  
  METHODS
  Twenty male Wistar rats were randomly divided into control group, model group, pre-acupuncture group and EA group, 5 rats in each. The model of simulated weightlessness was established by tail suspension for 4 week. One week before the tail suspension, the rats in the pre-acupuncture group were treated with electroacupuncture (EA) at "Shenshu" (BL 23), "Pishu"(BL 20) and "Sanyinjiao" (SP 6) for 30 min before treatment, once a day for 7 days. The rats in the EA group received tail suspension and acupuncture intervention at the same time. EA was applied for 30 min per treatment, once every other day for 14 times. Immunohistochemical staining was used to assay the expression of HSP 70 in the liver tissue. The activities of SOD and GSH-PX and content of MDA in liver tissues were examined by means of colourimetric method. Results Compared with the control group,the expression of HSP 70 and the content of MDA in the liver tissue were increased significantly (P < 0.01), and the activity of SOD and GSH-PX was notably reduced (P < 0.05) in the model group. Compared with the model group, the content of HSP 70 was significantly reduced in the pre-acupuncture group (P < 0.01). There were no significant changes in the levels of SOD, GSH-PX, MDA and HSP 70 in the EA group (P > 0.05). In comparison with the pre-acupuncture group, the activity of GSH-PX was lower (P < 0.05) and the content of MDA was higher (P < 0.05) in the EA group.
  
  
  CONCLUSIONS
  EA-pretreatment can suppress the increase of liver HSP 70 immunoactivity in rats with simulated weightlessness, being likely to improve the antioxidant ability of liver.
authors.forename: Yan
authors.initials: Y
authors.lastname: Song
authors.forename: Guo-zhen
authors.initials: GZ
authors.lastname: Zhao
authors.forename: Bai-xiao
authors.initials: BX
authors.lastname: Zhao
authors.forename: Bo
authors.initials: B
authors.lastname: Ji
authors.forename: De-sheng
authors.initials: DS
authors.lastname: Wang
authors.forename: He
authors.initials: H
authors.lastname: Zhang
authors.forename: Ying-qiu
authors.initials: YQ
authors.lastname: Mao
authors.forename: Ping
authors.initials: P
authors.lastname: Zhang
authors.forename: Yong-si
authors.initials: YS
authors.lastname: Xu
authors.forename: Ya-li
authors.initials: YL
authors.lastname: Liu
authors.forename: Ya-wen
authors.initials: YW
authors.lastname: Lu
authors.forename: Jian
authors.initials: J
authors.lastname: Dai
authors.forename: Ying-hui
authors.initials: YH
authors.lastname: Li
mesh_terms: D015669:Acupuncture Points; D000818:Animals; D015671:Electroacupuncture; D005979:Glutathione Peroxidase / Q000235:genetics / Q000378:metabolism*; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008099:Liver / Q000293:injuries / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism*; D008954:Models, Biological; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism*; D014893:Weightlessness / Q000009:adverse effects*
chemical_list: D018840:HSP70 Heat-Shock Proteins; D008315:Malondialdehyde; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase