title: From the Macro to the Micro: Gel Mapping to Differentiate between Sporozoites of Two Immunologically Distinct Strains of Eimeria maxima (Strains M6 and Guelph).
abstract: Two immunologically distinct strains of E. maxima were examined in this study: the M6 strain and the Guelph strain. The differential expression between the sporozoites of the two strains of E. maxima was determined by image analysis of 100 μg of protein from each strain separated by standard one- and conventional two-dimensional polyacrylamide gel electrophoresis. In addition to differences in both molecular weight and the electrophoretic mobility, differences in the intensity of polypeptide bands for example, GS 136.4 and M6 169 were explored. Pooled gels were prepared from each strain. A representative 2D-PAGE gel spanning a non-linear pH range of 3-10 of E. maxima strain M6 consisted of approximately 694 polypeptide spots with about 67 (9.6%) of the polypeptide spots being unique relative to the other strain. E. maxima strain GS had about 696 discernable polypeptide spots with 69 spots (9.9%) that differed from those of the M6 strain. In-depth characterization of the variable polypeptide spots; unique polypeptide spots (absence or presence) and shared polypeptide spots with modifications may lead to novel vaccine target in the form of multi-component, multi-stage, multi-immunovariant strains, multi-species subunit vaccine, and diagnostic probe for E. maxima.
authors.affiliation: State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing, 100193, China.|National Animal Protozoa Laboratory & College of Veterinary Medicine, China Agricultural University, Beijing, China.|Faculty of Science, Kafr El-Sheikh University, Kafr El-Sheikh, Egypt.|University of Guelph, Guelph, Ontario, Canada.
authors.forename: Saeed
authors.initials: S
authors.lastname: El-Ashram
authors.affiliation: State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing, 100193, China.|National Animal Protozoa Laboratory & College of Veterinary Medicine, China Agricultural University, Beijing, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Yin
authors.affiliation: Department of Pharmacology, Hebei North University, Zhangjiakou, Hebei, China.
authors.forename: Hongbin
authors.initials: H
authors.lastname: Liu
authors.affiliation: College of Applied Health Sciences in arras, Qassim, Qassim University, Saudi Arabia.
authors.forename: Ibrahim
authors.initials: I
authors.lastname: Al Nasr
authors.affiliation: State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing, 100193, China.|National Animal Protozoa Laboratory & College of Veterinary Medicine, China Agricultural University, Beijing, China.
authors.forename: Xianyong
authors.initials: X
authors.lastname: Liu
authors.affiliation: State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing, 100193, China.|National Animal Protozoa Laboratory & College of Veterinary Medicine, China Agricultural University, Beijing, China.
authors.forename: Xun
authors.initials: X
authors.lastname: Suo
authors.affiliation: University of Guelph, Guelph, Ontario, Canada.
authors.forename: John
authors.initials: J
authors.lastname: Barta
mesh_terms: D000818:Animals; D002645:Chickens / Q000469:parasitology; D004539:Eimeria / Q000235:genetics / Q000276:immunology*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D034101:Sporozoites / Q000276:immunology*