title: microRNA-342-5p and miR-608 inhibit colon cancer tumorigenesis by targeting NAA10.
abstract: miRNAs have been shown to play pivotal roles in the establishment and progression of colon cancer, but their underlying mechanisms are not fully understood. N-acetyltransferase NAA10 participates in many cellular processes, including tumorigenesis. Here we showed that miR-342-5p and miR-608 suppressed the tumorigenesis of colon cancer cells in vitro and in vivo by targeting NAA10 mRNA for degradation. Overexpression of miR-342-5p or miR-608 decreased NAA10 mRNA and protein levels and thereby suppressed cell proliferation, migration, and cell-cycle progression, as well as promoted apoptosis in SW480 and SW620 cells. More importantly, miR-342-5p and miR-608 significantly decreased the tumorigenic capacity of SW480 and SW620 cells in a mouse xenograft model. We also observed an inverse correlation between the expression of NAA10 and that of both miRNAs. Our results implicate miR-342-5p and miR-608 in colon cancer development and unveil the underlying mechanism of this phenomenon, which involves NAA10.
authors.affiliation: The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, China.
authors.forename: Hongju
authors.initials: H
authors.lastname: Yang
authors.affiliation: Human Genetics Center of Yunnan University, Kunming, Yunnan, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Li
authors.affiliation: Institute of Medicinal Biology Chinese Academy of Medical Science, Kunming, Yunnan, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Niu
authors.affiliation: Human Genetics Center of Yunnan University, Kunming, Yunnan, China.
authors.forename: Bai
authors.initials: B
authors.lastname: Li
authors.affiliation: The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, China.
authors.forename: Dejun
authors.initials: D
authors.lastname: Jiang
authors.affiliation: The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, China.
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Wan
authors.affiliation: The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, China.
authors.forename: Qingmei
authors.initials: Q
authors.lastname: Yang
authors.affiliation: The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Jiang
authors.affiliation: The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Wei
authors.affiliation: The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, China.
authors.forename: Song
authors.initials: S
authors.lastname: Bai
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D003110:Colonic Neoplasms / Q000235:genetics* / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D051379:Mice; D016513:Mice, SCID; D035683:MicroRNAs / Q000235:genetics*; D063209:N-Terminal Acetyltransferase A / Q000235:genetics* / Q000378:metabolism; D063213:N-Terminal Acetyltransferase E / Q000235:genetics* / Q000378:metabolism; D023041:Xenograft Model Antitumor Assays
chemical_list: C546176:MIRN342 microRNA, human; C000591804:MIRN608 microRNA, human; D035683:MicroRNAs; D063209:N-Terminal Acetyltransferase A; C504824:NAA10 protein, human; D063213:N-Terminal Acetyltransferase E
keywords: NAA10; colon cancer; miR-342-5p; miR-608