title: miR-548d-3p/TP53BP2 axis regulates the proliferation and apoptosis of breast cancer cells.
abstract: Fast growth and hardly any apoptosis are important characteristics of breast cancer, which assure the spread via invasion and metastasis of breast cancer cells. Inhibition of fast proliferation and induction of apoptosis are critical way to cure this cancer. microRNAs (miRNAs) had been increasingly reported to be the critical regulator of tumorigenesis. In our study, we found that increasing copy number of miR-548d-2-3p is critically involved poor prognosis. We overexpressed miR-548d-3p in MDA-MB-231cells and found that the proliferation was promoted significantly, whereas the inhibition of miR-548d-3p repressed the proliferation of MDA-MB-231 cells and also induced the increase in apoptosis. Additionally, we found that miR-548d-3p downregulated the expression of TP53BP2 by directly targeting the 3'UTR. We also found that knockdown of TP53BP2 significantly resorted the proliferation and apoptosis regulated by miR-548d-3p inhibitor. Our study showed that miR-548d-3p/TP53BP2 pathway is critically involved in the proliferation and apoptosis of breast cancer cells and may be new therapeutic target of breast cancer cells.
authors.affiliation: Department of Anesthesiology, Zhengzhou Central Hospital Affiliated to Zhengzhou University, 195 Tongbai Road, Zhengzhou, Henan, 450007, China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Song
authors.affiliation: Department of Anesthesiology, Zhengzhou Central Hospital Affiliated to Zhengzhou University, 195 Tongbai Road, Zhengzhou, Henan, 450007, China.
authors.forename: Jiangqiang
authors.initials: J
authors.lastname: Song
authors.affiliation: Department of Anesthesiology, Zhengzhou Central Hospital Affiliated to Zhengzhou University, 195 Tongbai Road, Zhengzhou, Henan, 450007, China.
authors.forename: Qimin
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Department of Anesthesiology, Zhengzhou Central Hospital Affiliated to Zhengzhou University, 195 Tongbai Road, Zhengzhou, Henan, 450007, China.
authors.forename: Yanling
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Department of Anesthesiology, Zhengzhou Central Hospital Affiliated to Zhengzhou University, 195 Tongbai Road, Zhengzhou, Henan, 450007, China.
authors.forename: Nai
authors.initials: N
authors.lastname: Sun
authors.affiliation: Department of Anesthesiology, Zhengzhou Central Hospital Affiliated to Zhengzhou University, 195 Tongbai Road, Zhengzhou, Henan, 450007, China.
authors.forename: Jieyu
authors.initials: J
authors.lastname: Ma
authors.affiliation: Department of Anesthesiology, Zhengzhou Central Hospital Affiliated to Zhengzhou University, 195 Tongbai Road, Zhengzhou, Henan, 450007, China.
authors.forename: Qiu
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Department of Anesthesiology, Zhengzhou Central Hospital Affiliated to Zhengzhou University, 195 Tongbai Road, Zhengzhou, Henan, 450007, China.
authors.forename: Guishan
authors.initials: G
authors.lastname: Xia
authors.affiliation: Department of Galactophore, Zhengzhou Central Hospital Affiliated to Zhengzhou University, 195 Tongbai Road, Zhengzhou, Henan, 450007, China.
authors.forename: Yanping
authors.initials: Y
authors.lastname: Huo
authors.affiliation: Department of Anesthesiology, Huangshi Central Hospita, Affiliated Hospital of Hubei Polytechnic University, Huangshi, 435000, China.
authors.forename: Longqiu
authors.initials: L
authors.lastname: Yang
authors.affiliation: Department of Anesthesiology, Zhengzhou Central Hospital Affiliated to Zhengzhou University, 195 Tongbai Road, Zhengzhou, Henan, 450007, China.
authors.forename: Baolin
authors.initials: B
authors.lastname: Li
mesh_terms: D020413:3' Untranslated Regions; D017209:Apoptosis / Q000235:genetics*; D051017:Apoptosis Regulatory Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D001665:Binding Sites; D001943:Breast Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000401:mortality / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D056915:DNA Copy Number Variations; D005260:Female; D018628:Gene Dosage; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D006801:Humans; D035683:MicroRNAs / Q000737:chemistry / Q000235:genetics*; D011379:Prognosis; D034622:RNA Interference; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics
chemical_list: D020413:3' Untranslated Regions; D051017:Apoptosis Regulatory Proteins; C561887:MIRN548 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; C090468:TP53BP2 protein, human
keywords: Apoptosis; TP53BP2; breast cancer; microRNA-548d; proliferation