title: Diversity and phylogeny of rhizobia associated with Desmodium spp. in Panxi, Sichuan, China.
abstract: Thirty-four rhizobial isolates were obtained from root nodules of four wild Desmodium species growing in Panxi, Sichuan, China. According to the combined ARDRA and IGS-RFLP (CACAI) cluster analysis, Rhizobium, Pararhizobium and Mesorhizobium isolates outnumbered Bradyrhizobium isolates. In general, the isolates representing the same species from the same site clustered together. Furthermore, the four Desmodium species were all nodulated by more than one rhizobial species. AFLP and phenotypic analyses showed that the 34 isolates represented at least 32 distinct strains. None of the strains were found from more than one site or host, indicating a high degree of rhizobial diversity in Panxi. In the multilocus sequence analysis, the isolates were assigned to Pararhizobium giardinii, Bradyrhizobium japonicum, Mesorhizobium septentrionale, and to undescribed species of the genera Rhizobium, Bradyrhizobium and Agrobacterium.
authors.affiliation: Department of Microbiology, College of Resources, Sichuan Agricultural University, Chengdu 625014, China. Electronic address: xkwei@sicau.edu.cn.
authors.forename: Kai Wei
authors.initials: KW
authors.lastname: Xu
authors.affiliation: Department of Microbiology, College of Resources, Sichuan Agricultural University, Chengdu 625014, China.
authors.forename: Lan
authors.initials: L
authors.lastname: Zou
authors.affiliation: Department of Environmental Sciences, University of Helsinki, Helsinki Fin-00014, Finland.
authors.forename: Petri
authors.initials: P
authors.lastname: Penttinen
authors.affiliation: Soil and Fertilizer Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China.
authors.forename: Xiangzhong
authors.initials: X
authors.lastname: Zeng
authors.affiliation: Department of Microbiology, College of Resources, Sichuan Agricultural University, Chengdu 625014, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Liu
authors.affiliation: Department of Microbiology, College of Resources, Sichuan Agricultural University, Chengdu 625014, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Zhao
authors.affiliation: Department of Microbiology, College of Resources, Sichuan Agricultural University, Chengdu 625014, China.
authors.forename: Cuiping
authors.initials: C
authors.lastname: Chen
authors.affiliation: Department of Microbiology, College of Resources, Sichuan Agricultural University, Chengdu 625014, China. Electronic address: cyxue2002@aliyun.com.
authors.forename: Yuan Xue
authors.initials: YX
authors.lastname: Chen
authors.affiliation: Department of Microbiology, College of Resources, Sichuan Agricultural University, Chengdu 625014, China. Electronic address: zhangxiaopingphd@126.com.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Zhang
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis; D001483:Base Sequence; D020369:Bradyrhizobium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D002681:China; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D007887:Fabaceae / Q000382:microbiology*; D059128:Mesorhizobium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D058885:Multilocus Sequence Typing; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S; D012231:Rhizobium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S
keywords: Desmodium; Genetic diversity; Housekeeping genes; MLSA; Rhizobia