title: Plasma Nitration of High-Density and Low-Density Lipoproteins in Chronic Kidney Disease Patients Receiving Kidney Transplants.
abstract: BACKGROUND
  Functional abnormalities of high-density lipoprotein (HDL) could contribute to cardiovascular disease in chronic kidney disease patients. We measured a validated marker of HDL dysfunction, nitrated apolipoprotein A-I, in kidney transplant recipients to test the hypothesis that a functioning kidney transplant reduces serum nitrated apoA-I concentrations.
  
  
  METHODS
  Concentrations of nitrated apoA-I and apoB were measured using indirect sandwich ELISA assays on sera collected from each transplant subject before transplantation and at 1, 3, and 12 months after transplantation. Patients were excluded if they have history of diabetes, treatment with lipid-lowering medications or HIV protease inhibitors, prednisone dose > 15 mg/day, nephrotic range proteinuria, serum creatinine > 1.5 mg/dL, or active inflammatory disease. Sera from 18 transplanted patients were analyzed. Four subjects were excluded due to insufficient data. Twelve and eight patients had creatinine < 1.5 mg/dL at 3 and 12 months after transplantation, respectively. RESULTS. Nitrated apoA-I was significantly reduced at 12 months after transplantation (p = 0.039). The decrease in apoA-I nitration was associated with significant reduction in myeloperoxidase (MPO) activity (p = 0.047). In contrast to apoA-I, nitrated apoB was not affected after kidney transplantation.
  
  
  CONCLUSIONS
  Patients with well-functioning grafts had significant reduction in nitrated apoA-I 12 months after kidney transplantation. Further studies are needed in a large cohort to determine if nitrated apoA-I can be used as a valuable marker for cardiovascular risk stratification in chronic kidney disease.
authors.affiliation: Department of Cell Biology, SUNY Downstate Medical Center, Brooklyn, NY, USA ; The Department of Veterans Affairs New York Harbor Healthcare System, Brooklyn, NY, USA.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Bakillah
authors.affiliation: Department of Medicine, SUNY Downstate Medical Center, Brooklyn, NY, USA.
authors.forename: Fasika
authors.initials: F
authors.lastname: Tedla
authors.affiliation: Department of Medicine, SUNY Downstate Medical Center, Brooklyn, NY, USA.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Ayoub
authors.affiliation: Department of Surgery, SUNY Downstate Medical Center, Brooklyn, NY, USA.
authors.forename: Devon
authors.initials: D
authors.lastname: John
authors.affiliation: Department of Cell Biology, SUNY Downstate Medical Center, Brooklyn, NY, USA ; Department of Medicine, SUNY Downstate Medical Center, Brooklyn, NY, USA.
authors.forename: Allen J
authors.initials: AJ
authors.lastname: Norin
authors.affiliation: Department of Cell Biology, SUNY Downstate Medical Center, Brooklyn, NY, USA ; The Department of Veterans Affairs New York Harbor Healthcare System, Brooklyn, NY, USA.
authors.forename: M Mahmood
authors.initials: MM
authors.lastname: Hussain
authors.affiliation: Department of Medicine, SUNY Downstate Medical Center, Brooklyn, NY, USA.
authors.forename: Clinton
authors.initials: C
authors.lastname: Brown
mesh_terms: D016632:Apolipoprotein A-I / Q000378:metabolism; D001055:Apolipoproteins B / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D016030:Kidney Transplantation; D015227:Lipid Peroxidation; D008075:Lipoproteins, HDL / Q000378:metabolism*; D008077:Lipoproteins, LDL / Q000378:metabolism*; D009195:Peroxidase / Q000378:metabolism; D051436:Renal Insufficiency, Chronic / Q000378:metabolism / Q000601:surgery*
chemical_list: D016632:Apolipoprotein A-I; D001055:Apolipoproteins B; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D009195:Peroxidase