title: Preparation, characterisation and antibacterial activity of a florfenicol-loaded solid lipid nanoparticle suspension.
abstract: A florfenicol-loaded solid lipid nanoparticle (FFC-SLN) suspension was prepared by hot homogenisation and ultrasonic technique. The suspension was characterised for its release profile, stability, toxicity, and the physicochemical properties of the nanoparticles. Antibacterial activity of the suspension was evaluated in vitro and in vivo. The results showed that the mean diameter, polydispersity index and zeta potential of the nanoparticles were 253 ± 3 nm, 0.409 ± 0.022 and 47.5 ± 0.21 mV, respectively. In vitro release profile showed the FFC-SLN suspension had sustained release effect. The minimum inhibition concentration values of the FFC-SLN suspension were 6 and 3 µg/mL against Staphylococcus aureus and Escherichia coli respectively, compared with 3.5 and 2 µg/mL of native florfenicol. The suspension was relatively stable at 4°C and less stable at room temperature during 9 months storage. Although the nanoparticle carriers exhibited cytotoxicity in cell cultures, the LD50 of the lyophilised dry power of the suspension was higher than 5 g/kg body weight. Mortality protection against E. coli lethal infection in mice showed that the nanoparticle suspension had much better efficacy (6/10) than native drug (1/10). These results indicate that FFC-SLN suspension could be a promising formulation in veterinary medicine.
authors.affiliation: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, China Agricultural University, 2 Yuanmingyuan Road West, Beijing 100193, People's Republic of China.
authors.forename: Ting
authors.initials: T
authors.lastname: Wang
authors.affiliation: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, China Agricultural University, 2 Yuanmingyuan Road West, Beijing 100193, People's Republic of China.
authors.forename: Xiaojin
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, China Agricultural University, 2 Yuanmingyuan Road West, Beijing 100193, People's Republic of China.
authors.forename: Mengmeng
authors.initials: M
authors.lastname: Lu
authors.affiliation: Inner Mongolia Saikexing Reproductive Biotechnology Co. Ltd., Inner Mongolia University College of Life Sciences, Shengle Economic District, Helin Geer, Huhhot, Inner Mongolia 011517, People's Republic of China.
authors.forename: Xihe
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, China Agricultural University, 2 Yuanmingyuan Road West, Beijing 100193, People's Republic of China. zhouwz@cau.edu.cn.
authors.forename: WenZhong
authors.initials: W
authors.lastname: Zhou
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000138:chemical synthesis; D018407:Bacterial Physiological Phenomena / Q000187:drug effects*; D002368:Castor Oil / Q000737:chemistry*; D002470:Cell Survival / Q000187:drug effects; D003692:Delayed-Action Preparations / Q000008:administration & dosage / Q000138:chemical synthesis*; D004058:Diffusion; D004305:Dose-Response Relationship, Drug; D004339:Drug Compounding / Q000379:methods; D004927:Escherichia coli Infections / Q000188:drug therapy* / Q000473:pathology; D005260:Female; D007928:Lethal Dose 50; D008297:Male; D051379:Mice; D053769:Nanocapsules / Q000008:administration & dosage / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D013535:Suspensions; D013839:Thiamphenicol / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D003692:Delayed-Action Preparations; D053769:Nanocapsules; D013535:Suspensions; D002368:Castor Oil; C035534:florfenicol; D013839:Thiamphenicol