title: Tenacibaculum finnmarkense sp. nov., a fish pathogenic bacterium of the family Flavobacteriaceae isolated from Atlantic salmon.
abstract: A novel Gram-stain negative, aerobic, non-flagellated, rod-shaped gliding bacterial strain, designated HFJ(T), was isolated from a skin lesion of a diseased Atlantic salmon (Salmo salar L.) in Finnmark, Norway. Colonies were observed to be yellow pigmented with entire and/or undulating margins and did not adhere to the agar. The 16S rRNA gene sequence showed that the strain belongs to the genus Tenacibaculum (family Flavobacteriaceae, phylum 'Bacteroidetes'). Strain HFJ(T) exhibits high 16S rRNA gene sequence similarity values to Tenacibaculum dicentrarchi NCIMB 14598(T) (97.2 %). The strain was found to grow at 2-20 °C and only in the presence of sea salts. The respiratory quinone was identified as menaquinone 6 and the major fatty acids were identified as summed feature 3 (comprising C16:1 ω7c and/or iso-C15:0 2-OH), iso-C15:0, anteiso-C15:0, iso-C15:1 and iso-C15:0 3-OH. The DNA G+C content was determined to be 34.1 mol%. DNA-DNA hybridization and comparative phenotypic and genetic tests were performed with the phylogenetically closely related type strains, T. dicentrarchi NCIMB 14598(T) and Tenacibaculum ovolyticum NCIMB 13127(T). These data, as well as phylogenetic analyses, suggest that strain HFJ(T) should be classified as a representative of a novel species in the genus Tenacibaculum, for which the name Tenacibaculum finnmarkense sp. nov. is proposed; the type strain is HFJ (T) = (DSM 28541(T) = NCIMB 42386(T)).
authors.affiliation: Fish Disease Research Group, Department of Biology, University of Bergen, Thormøhlensgt 55, Bergen, 5020, Norway. sverre.smage@cermaq.com.|Cermaq Group AS, Dronning Eufemias Gate 16, Oslo, 0191, Norway. sverre.smage@cermaq.com.
authors.forename: Sverre Bang
authors.initials: SB
authors.lastname: Småge
authors.affiliation: Cermaq Group AS, Dronning Eufemias Gate 16, Oslo, 0191, Norway.
authors.forename: Øyvind Jakobsen
authors.initials: ØJ
authors.lastname: Brevik
authors.affiliation: Cermaq Group AS, Dronning Eufemias Gate 16, Oslo, 0191, Norway.
authors.forename: Henrik
authors.initials: H
authors.lastname: Duesund
authors.affiliation: Cermaq Norway, Gjerbakknes, Nordfold, 8286, Norway.
authors.forename: Karl Fredrik
authors.initials: KF
authors.lastname: Ottem
authors.affiliation: Fish Disease Research Group, Department of Biology, University of Bergen, Thormøhlensgt 55, Bergen, 5020, Norway.
authors.forename: Kuninori
authors.initials: K
authors.lastname: Watanabe
authors.affiliation: Fish Disease Research Group, Department of Biology, University of Bergen, Thormøhlensgt 55, Bergen, 5020, Norway.
authors.forename: Are
authors.initials: A
authors.lastname: Nylund
mesh_terms: D000818:Animals; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism; D005393:Fish Diseases / Q000382:microbiology*; D045826:Flavobacteriaceae Infections / Q000382:microbiology / Q000662:veterinary*; D008969:Molecular Sequence Data; D009664:Norway; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D020317:Salmo salar / Q000382:microbiology*; D060493:Tenacibaculum / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S
keywords: Norway; Polyphasic taxonomy; Salmon farming; Skin lesions; Ulcerative disease; Winter ulcers