title: Turtle hatchlings show behavioral types that are robust to developmental manipulations.
abstract: There can be substantial variation among individuals within a species in how they behave, even under similar conditions; this pattern is found in many species and across taxa. However, the mechanisms that give rise to this behavioral variation are often unclear. This study investigated the influence of environmental manipulations during development on behavioral variation in hatchlings of the red-eared slider turtle (Trachemys scripta). First, we examined the effects of three manipulations during incubation (estrone sulfate exposure, corticosterone exposure, and thermal fluctuations) on hatchling righting response and exploration. Second, we determined whether hatchlings showed consistent differences (i.e. behavioral types) in their righting response and exploration across days and months, and whether these behaviors were correlated with one another. Finally, we examined whether righting response was predictive of ecologically relevant behaviors such as habitat choice and dispersal. Hatchling behavior was robust to our early manipulations; none of the pre-hatch treatments affected later behavior. There were significant clutch effects, which due to the split-clutch design suggests genetic underpinnings and/or maternal effects. We found evidence for behavioral types in turtles; both righting response and exploration were strongly repeatable and these behaviors were positively correlated. Righting response was not predictive of dispersal ability in the field, necessitating a revision in the general interpretations of righting response as a proxy for dispersal ability in turtles. Thus, turtle hatchlings show consistent behavioral differences that are robust to early developmental manipulations, and while not necessarily predictive of dispersal, these behavioral types can have important consequences throughout ontogeny.
authors.affiliation: School of Biological Sciences, Illinois State University, Julian Hall 210, Campus Box 4120, Normal, IL 61790, United States. Electronic address: afwilso@ilstu.edu.
authors.forename: A W
authors.initials: AW
authors.lastname: Carter
authors.affiliation: School of Biological Sciences, Illinois State University, Julian Hall 210, Campus Box 4120, Normal, IL 61790, United States. Electronic address: rpaitz@ilstu.edu.
authors.forename: R T
authors.initials: RT
authors.lastname: Paitz
authors.affiliation: School of Integrative Biology, University of Illinois, 286 Morrill Hall MC-120 505 S. Goodwin Ave., Urbana, IL 61801, United States; Department of Zoology, University of Cambridge, Downing Street, Cambridge CB2 3EJ, United Kingdom. Electronic address: kemcghee@sewanee.edu.
authors.forename: K E
authors.initials: KE
authors.lastname: McGhee
authors.affiliation: School of Biological Sciences, Illinois State University, Julian Hall 210, Campus Box 4120, Normal, IL 61790, United States. Electronic address: rmbowde@ilstu.edu.
authors.forename: R M
authors.initials: RM
authors.lastname: Bowden
mesh_terms: D000818:Animals; D002755:Choice Behavior / Q000187:drug effects / Q000502:physiology; D003345:Corticosterone / Q000008:administration & dosage / Q000378:metabolism; D017753:Ecosystem; D004970:Estrone / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000378:metabolism; D005106:Exploratory Behavior / Q000187:drug effects / Q000502:physiology*; D005260:Female; D007087:Illinois; D008297:Male; D009043:Motor Activity / Q000187:drug effects / Q000502:physiology*; D014426:Turtles / Q000254:growth & development* / Q000502:physiology*
chemical_list: D004970:Estrone; C017296:estrone sulfate; D003345:Corticosterone
keywords: Behavioral type; Dispersal; Exploration; Incubation; Righting response; Steroids