title: Decellularized Renal Matrix and Regenerative Medicine of the Kidney: A Different Point of View.
abstract: Over the past years, extracellular matrix (ECM) obtained from whole organ decellularization has been investigated as a platform for organ engineering. The ECM is composed of fibrous and nonfibrous molecules providing structural and biochemical support to the surrounding cells. Multiple decellularization techniques, including ours, have been optimized to maintain the composition, microstructure, and biomechanical properties of the native renal ECM that are difficult to obtain during the generation of synthetic substrates. There are evidences suggesting that in vivo implanted renal ECM has the capacity to induce formation of vasculature-like structures, but long-term in vivo transplantation and filtration activity by these tissue-engineered constructs have not been investigated or reported. Therefore, even if the process of renal decellularization is possible, the repopulation of the renal matrix with functional renal cell types is still very challenging. This review aims to summarize the current reports on kidney tissue engineering with the use of decellularized matrices and addresses the challenges in creating functional kidney units. Finally, this review discusses how future studies investigating cell-matrix interaction may aid the generation of a functional renal unit that would be transplantable into patients one day.
authors.affiliation: 1 Department of Development, Stem Cells and Regenerative Medicine, University of Southern California , Los Angeles, California.
authors.forename: Astgik
authors.initials: A
authors.lastname: Petrosyan
authors.affiliation: 2 Department of Urology, Children's Hospital Los Angeles , Los Angeles, California.
authors.forename: Ilenia
authors.initials: I
authors.lastname: Zanusso
authors.affiliation: 2 Department of Urology, Children's Hospital Los Angeles , Los Angeles, California.
authors.forename: Maria
authors.initials: M
authors.lastname: Lavarreda-Pearce
authors.affiliation: 2 Department of Urology, Children's Hospital Los Angeles , Los Angeles, California.
authors.forename: Scott
authors.initials: S
authors.lastname: Leslie
authors.affiliation: 2 Department of Urology, Children's Hospital Los Angeles , Los Angeles, California.
authors.forename: Sargis
authors.initials: S
authors.lastname: Sedrakyan
authors.affiliation: 2 Department of Urology, Children's Hospital Los Angeles , Los Angeles, California.
authors.forename: Roger E
authors.initials: RE
authors.lastname: De Filippo
authors.affiliation: 3 Department of General Surgery, Wake Forest School of Medicine , Winston Salem, North Carolina.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Orlando
authors.affiliation: 2 Department of Urology, Children's Hospital Los Angeles , Los Angeles, California.
authors.forename: Stefano
authors.initials: S
authors.lastname: Da Sacco
authors.affiliation: 2 Department of Urology, Children's Hospital Los Angeles , Los Angeles, California.
authors.forename: Laura
authors.initials: L
authors.lastname: Perin
mesh_terms: D005109:Extracellular Matrix; D006801:Humans; D007668:Kidney; D044968:Regenerative Medicine; D023822:Tissue Engineering; D054457:Tissue Scaffolds