title: Stochasticity and homeostasis in the E. coli replication and division cycle.
abstract: How cells correct for stochasticity to coordinate the chromosome replication and cellular division cycle is poorly understood. We used time-lapse microscopy and fluorescently labelled SeqA to determine the timing of birth, initiation, termination, and division, as well as cell size throughout the cell cycle. We found that the time between birth and initiation (B-period) compensates for stochastic variability in birth size and growth rate. The time between termination and division (D-period) also compensates for size and growth variability, invalidating the notion that replication initiation is the principal trigger for cell division. In contrast, the time between initiation and termination (C-period) did not display such compensations. Interestingly, the C-period did show small but systematic decreases for cells that spontaneously grew faster, which suggests a coupling between metabolic fluctuations and replication. An auto-regressive theoretical framework was employed to compare different possible models of sub-period control.
authors.affiliation: FOM Institute AMOLF, 1098 XG Amsterdam, the Netherlands.
authors.forename: Aileen
authors.initials: A
authors.lastname: Adiciptaningrum
authors.affiliation: Dipartimento di Fisica and INFN, University of Torino, V. Pietro Giuria 1, Torino, I-10125, Italy.
authors.forename: Matteo
authors.initials: M
authors.lastname: Osella
authors.affiliation: Department of Biochemistry, University of Oxford, Oxford OX1 3QU, United Kingdom.
authors.forename: M Charl
authors.initials: MC
authors.lastname: Moolman
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06, UMR 7238, Computational and Quantitative Biology, 15 rue de l'École de Médecine, Paris, France.|CNRS, UMR 7238 Paris, France.
authors.forename: Marco
authors.initials: M
authors.lastname: Cosentino Lagomarsino
authors.affiliation: FOM Institute AMOLF, 1098 XG Amsterdam, the Netherlands.
authors.forename: Sander J
authors.initials: SJ
authors.lastname: Tans
mesh_terms: D002453:Cell Cycle; D002455:Cell Division; D004261:DNA Replication; D004926:Escherichia coli / Q000502:physiology*; D006706:Homeostasis; D050296:Microbial Viability; D008954:Models, Biological