title: In vitrogene expression profile of bovine peripheral blood mononuclear cells in earlyMycobacterium bovisinfection.
abstract: The intracellular parasiteMycobacterium bovis(M. bovis) causes tuberculosis in cattle and humans. Understanding the interactions betweenM. bovisand host cells is essential in developing tools for the prevention, detection, and treatment of M. bovis infection. Gene expression profiles provide a large amount of information regarding the molecular mechanisms underlying these interactions. The present study analyzed changes in gene expression in bovine peripheral blood mononuclear cells (PBMCs) at 0, 4 and 24 h following exposure to M. bovis. Using bovine whole-genome microarrays, a total of 420 genes were identified that exhibited significant alterations in expression (≥2-fold). Significantly enriched genes were identified using the Kyoto Encyclopedia of Genes and Genomes database, of which the highest differentially expressed genes were associated with the immune system, signal transduction, endocytosis, cellular transport, inflammation, and apoptosis. Of the genes associated with the immune system, 84.85% displayed downregulation. These findings support the view that M. bovis inhibits signaling pathways of antimycobacterial host defense in bovine PBMCs. Thesein vitrodata demonstrated that molecular alterations underlying the pathogenesis of tuberculosis begin early, during the initial 24 h following M. bovis infection.
authors.affiliation: School of Veterinary Medicine, National Taiwan University, Taipei 10617, Taiwan, R.O.C. ; Centers for Disease Control, Ministry of Health and Welfare, Taipei 10050, Taiwan, R.O.C.
authors.forename: Yafen
authors.initials: Y
authors.lastname: Cheng
authors.affiliation: School of Veterinary Medicine, National Taiwan University, Taipei 10617, Taiwan, R.O.C.
authors.forename: Chung-Hsi
authors.initials: CH
authors.lastname: Chou
authors.affiliation: School of Veterinary Medicine, National Taiwan University, Taipei 10617, Taiwan, R.O.C. ; Animal Health Research Institute, Council of Agriculture, Executive Yuan, New Taipei 25118, Taiwan, R.O.C.
authors.forename: Hsiang-Jung
authors.initials: HJ
authors.lastname: Tsai
keywords: Mycobacterium bovis; global gene expression; immune response; macrophage