title: The Scion/Rootstock Genotypes and Habitats Affect Arbuscular Mycorrhizal Fungal Community in Citrus.
abstract: Citrus roots have rare root hairs and thus heavily depend on arbuscular mycorrhizal fungi (AMF) for mineral nutrient uptake. However, the AMF community structure of citrus is largely unknown. By using 454-pyrosequencing of 18S rRNA gene fragment, we investigated the genetic diversity of AMF colonizing citrus roots, and evaluated the impact of habitats and rootstock and scion genotypes on the AMF community structure. Over 7,40,000 effective sequences were obtained from 77 citrus root samples. These sequences were assigned to 75 AMF virtual taxa, of which 66 belong to Glomus, highlighting an absolute dominance of this AMF genus in symbiosis with citrus roots. The citrus AMF community structure is significantly affected by habitats and host genotypes. Interestingly, our data suggests that the genotype of the scion exerts a greater impact on the AMF community structure than that of the rootstock where the physical root-AMF association occurs. This study not only provides a comprehensive assessment for the community composition of the AMF in citrus roots under different conditions, but also sheds novel insights into how the AMF community might be indirectly influenced by the spatially separated yet metabolically connected partner-the scion-of the grafted citrus tree.
authors.affiliation: Key Laboratory of Horticultural Plant Biology (Ministry of Education), Key Laboratory of Horticultural Crop Biology and Genetic Improvement (Central Region, Ministry of Agriculture), College of Horticulture and Forestry Sciences, Huazhong Agricultural University Wuhan, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Song
authors.affiliation: Key Laboratory of Horticultural Plant Biology (Ministry of Education), Key Laboratory of Horticultural Crop Biology and Genetic Improvement (Central Region, Ministry of Agriculture), College of Horticulture and Forestry Sciences, Huazhong Agricultural University Wuhan, China.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Pan
authors.affiliation: Key Laboratory of Horticultural Plant Biology (Ministry of Education), Key Laboratory of Horticultural Crop Biology and Genetic Improvement (Central Region, Ministry of Agriculture), College of Horticulture and Forestry Sciences, Huazhong Agricultural University Wuhan, China.
authors.forename: Fuxi
authors.initials: F
authors.lastname: Bai
authors.affiliation: Key Laboratory of Horticultural Plant Biology (Ministry of Education), Key Laboratory of Horticultural Crop Biology and Genetic Improvement (Central Region, Ministry of Agriculture), College of Horticulture and Forestry Sciences, Huazhong Agricultural University Wuhan, China.
authors.forename: Jianyong
authors.initials: J
authors.lastname: An
authors.affiliation: Key Laboratory of Horticultural Plant Biology (Ministry of Education), Key Laboratory of Horticultural Crop Biology and Genetic Improvement (Central Region, Ministry of Agriculture), College of Horticulture and Forestry Sciences, Huazhong Agricultural University Wuhan, China.
authors.forename: Jihong
authors.initials: J
authors.lastname: Liu
authors.affiliation: Key Laboratory of Horticultural Plant Biology (Ministry of Education), Key Laboratory of Horticultural Crop Biology and Genetic Improvement (Central Region, Ministry of Agriculture), College of Horticulture and Forestry Sciences, Huazhong Agricultural University Wuhan, China.
authors.forename: Wenwu
authors.initials: W
authors.lastname: Guo
authors.affiliation: Laboratory of Molecular Biology, Department of Plant Sciences, Wageningen University Wageningen, Netherlands.
authors.forename: Ton
authors.initials: T
authors.lastname: Bisseling
authors.affiliation: Key Laboratory of Horticultural Plant Biology (Ministry of Education), Key Laboratory of Horticultural Crop Biology and Genetic Improvement (Central Region, Ministry of Agriculture), College of Horticulture and Forestry Sciences, Huazhong Agricultural University Wuhan, China.
authors.forename: Xiuxin
authors.initials: X
authors.lastname: Deng
authors.affiliation: Key Laboratory of Horticultural Plant Biology (Ministry of Education), Key Laboratory of Horticultural Crop Biology and Genetic Improvement (Central Region, Ministry of Agriculture), College of Horticulture and Forestry Sciences, Huazhong Agricultural University Wuhan, China ; Department of Plant Science and Landscape Architecture, Institute for Bioscience and Biotechnology Research, University of Maryland Rockville, MD, USA.
authors.forename: Shunyuan
authors.initials: S
authors.lastname: Xiao
keywords: arbuscular mycorrhizal fungi (AMF); citrus; community structure; habitat; scion/rootstock genotype