title: [Testing of apathogenic influenza virus H5N3 as a poultry live vaccine].
abstract: Four H5N2 experimental vaccine strains and the apathogenic wild duck H5N3 influenza virus A/duck/ Moscow/4182/2010 (dk/4182) were tested as a live poultry vaccine. Experimental strains had the hemagglutinin of the A/Vietnam/1203/04 strain lacking the polybasic HA cleavage site or the hemagglutinin from attenuated virus (Ku/ at) that was derived from the highly pathogenic influenza virus A/chicken/Kurgan/3/2005 (H5N1). The hemagglutinin of the Ku-at has the amino acid substitutions Asp54/Asn and Lys222/Thr in HA1 and Val48/Ile and Lys131/Thr in HA2, while maintaining the polybasic HA cleavage site at an invariable level. The other genes of these experimental strains were from the H2N2 cold-adapted master strain A/Leningrad/134/17/57 (VN-Len and Ku-Len) or from the apathogenic H6N2 virus A/gull/Moscow/3100/2006 (VN-Gull and Ku-Gull). A single immunization of mice with all tested strains elicited a high level of serum antibodies and provided complete protection against the challenge with the lethal dose of A/chicken/Kurgan/3/05. The pathogenicity indexes of the Ku-at and the other strains for chicken were virtually zero, whereas the index of the parent H5N1 virus A/chicken/Kurgan/3/2005 was 2.98. Intravenous, intranasal, and aerosol routes of vaccination were compared. It was shown that the strain dk/4182 was totally apathogenic for one-day-old chicken and provided complete protection against the highly pathogenic H5N1 virus.
authors.forename: E Y
authors.initials: EY
authors.lastname: Boravleva
authors.forename: I A
authors.initials: IA
authors.lastname: Chvala
authors.forename: N F
authors.initials: NF
authors.lastname: Lomakina
authors.forename: P I
authors.initials: PI
authors.lastname: Repin
authors.forename: N S
authors.initials: NS
authors.lastname: Mudrak
authors.forename: L G
authors.initials: LG
authors.lastname: Rudenko
authors.forename: A S
authors.initials: AS
authors.lastname: Gambaryan
authors.forename: V V
authors.initials: VV
authors.lastname: Drygin
mesh_terms: D000818:Animals; D002645:Chickens / Q000276:immunology / Q000821:virology; D009980:Influenza A virus / Q000276:immunology*; D007252:Influenza Vaccines / Q000276:immunology*; D005585:Influenza in Birds / Q000276:immunology / Q000517:prevention & control; D051379:Mice; D011201:Poultry Diseases / Q000276:immunology / Q000517:prevention & control; D014613:Vaccines, Attenuated / Q000276:immunology
chemical_list: D007252:Influenza Vaccines; D014613:Vaccines, Attenuated