title: Virulence Genes in Expanded-Spectrum-Cephalosporin-Resistant and -Susceptible Escherichia coli Isolates from Treated and Untreated Chickens.
abstract: This study investigated antimicrobial resistance, screened for the presence of virulence genes involved in intestinal infections, and determined phylogenetic groups of Escherichia coli isolates from untreated poultry and poultry treated with ceftiofur, an expanded-spectrum cephalosporin. Results show that none of the 76 isolates appeared to be Shiga toxin-producing E. coli or enteropathogenic E. coli. All isolates were negative for the major virulence factors/toxins tested (ehxA, cdt, heat-stable enterotoxin [ST], and heat-labile enterotoxin [LT]). The few virulence genes harbored in isolates generally did not correlate with isolate antimicrobial resistance or treatment status. However, some of the virulence genes were significantly associated with certain phylogenetic groups.
authors.affiliation: ANSES, Ploufragan Laboratory, Ploufragan, France Université Européenne de Bretagne, Rennes, France.
authors.forename: S
authors.initials: S
authors.lastname: Baron
authors.affiliation: Université Paris-Est, ANSES, Food Safety Laboratory, Platform IdentyPath, Maisons-Alfort, France.
authors.forename: S
authors.initials: S
authors.lastname: Delannoy
authors.affiliation: ANSES, Ploufragan Laboratory, Ploufragan, France Université Européenne de Bretagne, Rennes, France.
authors.forename: S
authors.initials: S
authors.lastname: Bougeard
authors.affiliation: ANSES, Ploufragan Laboratory, Ploufragan, France Université Européenne de Bretagne, Rennes, France.
authors.forename: E
authors.initials: E
authors.lastname: Larvor
authors.affiliation: ANSES, Ploufragan Laboratory, Ploufragan, France Université Européenne de Bretagne, Rennes, France.
authors.forename: E
authors.initials: E
authors.lastname: Jouy
authors.affiliation: ANSES, Ploufragan Laboratory, Ploufragan, France Université Européenne de Bretagne, Rennes, France.
authors.forename: O
authors.initials: O
authors.lastname: Balan
authors.affiliation: Université Paris-Est, ANSES, Food Safety Laboratory, Platform IdentyPath, Maisons-Alfort, France.
authors.forename: P
authors.initials: P
authors.lastname: Fach
authors.affiliation: ANSES, Ploufragan Laboratory, Ploufragan, France Université Européenne de Bretagne, Rennes, France isabelle.kempf@anses.fr.
authors.forename: I
authors.initials: I
authors.lastname: Kempf
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000627:therapeutic use*; D002511:Cephalosporins / Q000627:therapeutic use*; D002645:Chickens / Q000382:microbiology*; D024901:Drug Resistance, Multiple, Bacterial; D004926:Escherichia coli / Q000187:drug effects* / Q000302:isolation & purification / Q000472:pathogenicity; D004927:Escherichia coli Infections / Q000188:drug therapy* / Q000382:microbiology; D008826:Microbial Sensitivity Tests; D011201:Poultry Diseases / Q000188:drug therapy* / Q000382:microbiology; D037521:Virulence Factors / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D002511:Cephalosporins; D037521:Virulence Factors; C053503:ceftiofur