title: Simultaneous HPLC Quantitative Analysis of Nine Bioactive Constituents in Scirpus Yagara Ohwi. (Cyperaceae).
abstract: The tuber of Scirpus yagara Ohwi. (Cyperaceae) has long been used in traditional Chinese medicine (TCM). Several chemical constituents isolated from it possess a variety of physiologically activities such as anti-inflammatory, antitumor and antioxidant. A simultaneous high-performance liquid chromatography (HPLC) analysis was developed and validated for the determination of nine active components in tubers and aerial parts of S. yagara. The analysis was performed on a YMC-Pack ODS-A column (4.6 × 250 mm, 5 μm, 30 nm) with a multilinear gradient mobile phase of water-formic acid (100 : 0.2, v/v) and methanol. The established HPLC method was validated in terms of linearity, sensitivity, precision, accuracy, recovery and stability. All analyzed components were detected in the whole tested samples, and the contents of most components in the aerial parts were even higher than those in the tubers. Moreover, the best harvest period was discovered to be November, which is different from the traditional. The method developed was successfully applied for simultaneous qualitative and quantitative analysis of nine active components in S. yagara.
authors.affiliation: Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine, Nanjing 210023, Jiangsu, China.
authors.forename: Jianfang
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine, Nanjing 210023, Jiangsu, China.
authors.forename: Nuoshu
authors.initials: N
authors.lastname: Zou
authors.affiliation: Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine, Nanjing 210023, Jiangsu, China liangqiaoli2008@163.com.
authors.forename: Qiaoli
authors.initials: Q
authors.lastname: Liang
authors.affiliation: Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine, Nanjing 210023, Jiangsu, China.
authors.forename: Yamin
authors.initials: Y
authors.lastname: Tang
authors.affiliation: Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine, Nanjing 210023, Jiangsu, China.
authors.forename: Jin'ao
authors.initials: J
authors.lastname: Duan
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002934:Cinnamates / Q000302:isolation & purification*; D029785:Cyperaceae / Q000737:chemistry*; D004365:Drugs, Chinese Herbal; D005561:Formates; D062385:Hydroxybenzoates / Q000302:isolation & purification*; D000432:Methanol; D035261:Plant Components, Aerial / Q000737:chemistry; D035281:Plant Tubers / Q000737:chemistry*; D015203:Reproducibility of Results; D012621:Seasons; D012680:Sensitivity and Specificity; D012997:Solvents; D014867:Water
chemical_list: D002934:Cinnamates; D004365:Drugs, Chinese Herbal; D005561:Formates; D062385:Hydroxybenzoates; D012997:Solvents; D014867:Water; C030544:formic acid; D000432:Methanol