title: DNP-KLH Yields Changes in Leukocyte Populations and Immunoglobulin Isotype Use with Different Immunization Routes in Zebrafish.
abstract: Distinct methods are required for inducing mucosal versus systemic immunity in mammals for vaccine protection at the tissues most commonly breached by pathogens. Understanding of mucosal immunization in teleost fish is needed to combat aquaculture disease, understand emerging ecological threats, and know how vertebrate adaptive immunity evolved. Here, we quantitatively measured expression levels of IgM as well as the teleost mucosal immunoglobulin, IgZ/IgT, in zebrafish given an antigen systemically via intraperitoneal (i.p.) injection or mucosally via bath immersion. Both immunoglobulin isotypes and the B cell activating factor gene transcription was induced in fish injected with antigen as compared to saline injected or antigen immersed fish, though these failed to reach statistical significance. Here we provide additional reference hematology for this model species. Differential blood counts revealed a greater lymphocyte percentage in both i.p. and immersed fish, with increase in large lymphocyte counts and decrease in neutrophils. These humoral adaptive gene transcription and cytological data should provide a foundation for more studies connecting immunology in this dominant developmental and genetic fish model to other species where mucosal immunization is of greater commercial importance.
authors.affiliation: Comparative Immunogenetics Laboratory, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University , College Station, TX , USA ; Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University , College Station, TX , USA ; Department of Science, A&M Consolidated High School , College Station, TX , USA.
authors.forename: Heather
authors.initials: H
authors.lastname: Weir
authors.affiliation: Comparative Immunogenetics Laboratory, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University , College Station, TX , USA ; Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University , College Station, TX , USA.
authors.forename: Patricia L
authors.initials: PL
authors.lastname: Chen
authors.affiliation: Comparative Immunogenetics Laboratory, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University , College Station, TX , USA ; Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University , College Station, TX , USA.
authors.forename: Thaddeus C
authors.initials: TC
authors.lastname: Deiss
authors.affiliation: Comparative Immunogenetics Laboratory, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University , College Station, TX , USA ; Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University , College Station, TX , USA.
authors.forename: Natalie
authors.initials: N
authors.lastname: Jacobs
authors.affiliation: Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University , College Station, TX , USA.
authors.forename: Mary B
authors.initials: MB
authors.lastname: Nabity
authors.affiliation: Comparative Immunogenetics Laboratory, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University , College Station, TX , USA ; Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University , College Station, TX , USA ; Department of Science, A&M Consolidated High School , College Station, TX , USA.
authors.forename: Matt
authors.initials: M
authors.lastname: Young
authors.affiliation: Comparative Immunogenetics Laboratory, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University , College Station, TX , USA ; Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University , College Station, TX , USA.
authors.forename: Michael F
authors.initials: MF
authors.lastname: Criscitiello
keywords: BAFF; IgZ; immersion immunization; mucosal; zebrafish