title: Quantitative DNA Methylation Profiling in Cancer.
abstract: Epigenetic mechanisms including DNA methylation are fundamental for the regulation of gene expression. Epigenetic alterations can lead to the development and the evolution of malignant tumors as well as the emergence of phenotypically different cancer cells or metastasis from one single tumor cell. Here we describe bisulfite pyrosequencing, a technology to perform quantitative DNA methylation analyses, to detect aberrant DNA methylation in malignant tumors.
authors.affiliation: Institute of Human Genetics, University of Kiel and University Hospital Schleswig-Holstein, Campus Kiel, Schwanenweg 24, Kiel, 24105, Germany. oammerpohl@medgen.uni-kiel.de.
authors.forename: Ole
authors.initials: O
authors.lastname: Ammerpohl
authors.affiliation: Institute of Human Genetics, University of Kiel and University Hospital Schleswig-Holstein, Campus Kiel, Schwanenweg 24, Kiel, 24105, Germany.
authors.forename: Andrea
authors.initials: A
authors.lastname: Haake
authors.affiliation: Institute of Human Genetics, University of Kiel and University Hospital Schleswig-Holstein, Campus Kiel, Schwanenweg 24, Kiel, 24105, Germany.
authors.forename: Julia
authors.initials: J
authors.lastname: Kolarova
authors.affiliation: Institute of Human Genetics, University of Kiel and University Hospital Schleswig-Holstein, Campus Kiel, Schwanenweg 24, Kiel, 24105, Germany.
authors.forename: Reiner
authors.initials: R
authors.lastname: Siebert
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000032:analysis / Q000235:genetics*; D016172:DNA Fingerprinting / Q000379:methods*; D019175:DNA Methylation; D044127:Epigenesis, Genetic; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D009369:Neoplasms / Q000235:genetics; D013447:Sulfites / Q000737:chemistry
chemical_list: D013447:Sulfites; D004247:DNA; C042345:hydrogen sulfite
keywords: Bisulfite pyrosequencing; DNA methylation profiling; Epigenetics