title: Mice lack of LRG-47 display the attenuated outcome of infection with Schistosoma japonicum.
abstract: Interferon-inducible GTPase LRG-47 (also named immune-related GTPase M, Irgm1) is a member of the p47 GTPase family that has been shown to regulate host resistance to intracellular pathogens. Little knowledge has been known about the role of LRG-47 in host's responses to extracellular pathogens. To investigate possible roles of LRG-47 in the course of Schistosoma japonicum infection, LRG-47-deficient (LRG-47(-/-)) and wild-type (WT) mice were challenged with cercariae of S. japonicum, and the cellular and humoral responses in mice were analyzed. At the acute stage of S. japonicum infection, in contrast to WT mice, LRG-47(-/-) mice showed the significantly decreased egg burden, low schistosome-specific antibody response, and the decreased Th1 and increased Tc1 responses. Additionally, Schistosoma japonicum-specific egg antigen immunization also produced the similar humoral and cellular immune responses as S. japonicum infection. Taken together, these data suggested that the deficiency of LRG-47 might affect host's CD4(+) T cell immune response via the weakening of IFN-γ downstream signaling; however, the specific function of LRG-47 on dealing with extracellular worm needs to be further studied.
authors.affiliation: Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu, 210029, China.|College of Basic Medicine, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, China.
authors.forename: Yanan
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu, 210029, China.|Department of Basic Medical Sciences, School of Medicine, Tsinghua University, Beijing, China.
authors.forename: Jingjiao
authors.initials: J
authors.lastname: Wu
authors.affiliation: Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu, 210029, China.
authors.forename: Meijuan
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu, 210029, China.
authors.forename: Min
authors.initials: M
authors.lastname: Hou
authors.affiliation: Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu, 210029, China. jiminjun@njmu.edu.cn.|Jiangsu Province Key Laboratory of Modern Pathogen Biology, Nanjing, Jiangsu, China. jiminjun@njmu.edu.cn.
authors.forename: Minjun
authors.initials: M
authors.lastname: Ji
mesh_terms: D000818:Animals; D019204:GTP-Binding Proteins / Q000235:genetics*; D051379:Mice; D012549:Schistosoma japonicum / Q000276:immunology; D012554:Schistosomiasis japonica / Q000235:genetics* / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: C096000:Ifi1 protein, mouse; D019204:GTP-Binding Proteins
keywords: Immune response; LRG-47; Schistosoma japonicum; p47 GTPases