title: The synthesis of hydroxyapatite with different crystallinities by controlling the concentration of recombinant CEMP1 for biological application.
abstract: Cementum protein 1 (CEMP1) has been reported to be cementum specific and it plays a role in the properties and structure of cementum mineralization. This study was carried out to test the hypothesis that, CEMP1 had the capacity to guide ordered HA formation as it accumulated at targeting site. Crystals induced by protein were prepared by biomimetic method. The effects of CEMP1 concentrations (0-100μg/ml) on the rate of calcium phosphate precipitation was monitored during 48h, while the formed mineral phases were assessed utilizing transmission electron microscope (TEM), selected area electron diffraction (SAED), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR). The results showed that recombinant cementum protein 1 (rhCEMP1) affected the crystal nucleation and growth. At lower rhCEMP1 concentrations (0-50μg/ml), limited mineral formation occurred and only small bundles of HA crystals were found. However, with 100μg/ml of rhCEMP1, a predominance of organized linear needle-like HA crystals was observed. FTIR revealed the characteristic bands of HA appearing at 1106cm(-1), 557cm(-1), and 598cm(-1). Therefore, increasing the concentration of rhCEMP1 can lead to the formation of ordered bundles of HA crystals in vitro.
authors.affiliation: Hospital of Stomatology, Medical School of Nanjing University, Nanjing, Jiangsu 210093, People's Republic of China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Chen
authors.affiliation: Hospital of Stomatology, Medical School of Nanjing University, Nanjing, Jiangsu 210093, People's Republic of China. Electronic address: lyvette@163.com.
authors.forename: Yu
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Hospital of Stomatology, Medical School of Nanjing University, Nanjing, Jiangsu 210093, People's Republic of China.
authors.forename: Jie
authors.initials: J
authors.lastname: Yang
authors.affiliation: Hospital of Stomatology, Medical School of Nanjing University, Nanjing, Jiangsu 210093, People's Republic of China.
authors.forename: Wenlei
authors.initials: W
authors.lastname: Wu
authors.affiliation: Hospital of Stomatology, Medical School of Nanjing University, Nanjing, Jiangsu 210093, People's Republic of China.
authors.forename: Leiying
authors.initials: L
authors.lastname: Miao
authors.affiliation: Hospital of Stomatology, Medical School of Nanjing University, Nanjing, Jiangsu 210093, People's Republic of China.
authors.forename: Yijun
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Biomaterials and Tissue Engineering Group, Leeds Dental Institute, University of Leeds, United Kingdom.
authors.forename: Xuebin
authors.initials: X
authors.lastname: Yang
authors.affiliation: Hospital of Stomatology, Medical School of Nanjing University, Nanjing, Jiangsu 210093, People's Republic of China. Electronic address: wbsun@nju.edu.cn.
authors.forename: Weibin
authors.initials: W
authors.lastname: Sun
mesh_terms: D003692:Delayed-Action Preparations / Q000737:chemistry; D017886:Durapatite / Q000737:chemistry*; D006801:Humans; D008422:Materials Testing; D011506:Proteins / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry
chemical_list: C510886:CEMP1 protein, human; D003692:Delayed-Action Preparations; D011506:Proteins; D011994:Recombinant Proteins; D017886:Durapatite
keywords: Calcium phosphate; Cementum protein 1; Crystal morphology; Mineralization