title: Characterization of Positively Charged Lipid Shell Microbubbles with Tunable Resistive Pulse Sensing (TRPS) Method: A Technical Note.
abstract: Microbubbles are polydisperse microparticles. Their size distribution cannot be accurately measured from the current methods used, such as optical microscopy, electrical sensing or light scattering. Indeed, these techniques present some limitations when applied to microbubbles, which prompted us to investigate the use of an alternative technique: tunable resistive pulse sensing (TRPS). This technique is based on the principle of the Coulter counter with the advantage of being more flexible compared to other methods using this principle, since the flow rate, the potential difference and the pore size can be modulated. The main limitation of TRPS is that more than one size of nanopore membrane is required to obtain the full size distribution of polydisperse microparticles. To evaluate this technique, the concentration and the size distribution of positively charged microbubbles were studied using TRPS and compared to data obtained using optical microscopy. We describe herein the parameters required for the accurate measurement of microbubble concentration and size distribution by TRPS and present a statistical comparison of the data obtained by TRPS and optical microscopy.
authors.affiliation: Paris Descartes University, Sorbonne Paris Cité, Team vectors for molecular imaging and targeted therapy, CNRS UTCBS UMR8258, INSERM UTCBS U1022, Chimie ParisTech, PSL Research University, Paris, France.
authors.forename: Simona
authors.initials: S
authors.lastname: Manta
authors.affiliation: Center for Molecular Biophysics (CBM), CNRS UPR4301, Orléans, France.
authors.forename: Anthony
authors.initials: A
authors.lastname: Delalande
authors.affiliation: Paris Descartes University, Sorbonne Paris Cité, Team vectors for molecular imaging and targeted therapy, CNRS UTCBS UMR8258, INSERM UTCBS U1022, Chimie ParisTech, PSL Research University, Paris, France.
authors.forename: Michel
authors.initials: M
authors.lastname: Bessodes
authors.affiliation: Paris Descartes University, Sorbonne Paris Cité, Team vectors for molecular imaging and targeted therapy, CNRS UTCBS UMR8258, INSERM UTCBS U1022, Chimie ParisTech, PSL Research University, Paris, France.
authors.forename: Michel Francis
authors.initials: MF
authors.lastname: Bureau
authors.affiliation: Paris Descartes University, Sorbonne Paris Cité, Team vectors for molecular imaging and targeted therapy, CNRS UTCBS UMR8258, INSERM UTCBS U1022, Chimie ParisTech, PSL Research University, Paris, France.
authors.forename: Daniel
authors.initials: D
authors.lastname: Scherman
authors.affiliation: Center for Molecular Biophysics (CBM), CNRS UPR4301, Orléans, France.
authors.forename: Chantal
authors.initials: C
authors.lastname: Pichon
authors.affiliation: Paris Descartes University, Sorbonne Paris Cité, Team vectors for molecular imaging and targeted therapy, CNRS UTCBS UMR8258, INSERM UTCBS U1022, Chimie ParisTech, PSL Research University, Paris, France. Electronic address: Nathalie.Mignet@parisdescartes.fr.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Mignet
mesh_terms: D004563:Electrochemistry / Q000379:methods*; D004574:Electromagnetic Fields; D008055:Lipids / Q000032:analysis*; D008567:Membranes, Artificial; D045423:Microbubbles; D058608:Nanopores / Q000648:ultrastructure*; D055672:Static Electricity; D014462:Ultrafiltration / Q000379:methods*
chemical_list: D008055:Lipids; D008567:Membranes, Artificial
keywords: Mean size diameter; Microbubble; Particle counting; Tunable Resistive Pulse Sensing (TRPS)