title: MicroRNA-130b promotes cell migration and invasion by targeting peroxisome proliferator-activated receptor gamma in human glioma.
abstract: MircroRNA-130b (miR-130b) has been recognized as an oncogenic miRNA and is implicated in the initiation and development of human cancers. Deregulation of miR-130b has been reported in several tumors. However, the clinical significance and its underlying role in human glioma are poorly explored. Herein, we found that the expression of miR-130b was significantly up-regulated in glioma tissues as compared with that in normal brain (NB) tissues. Clinical association analysis disclosed that high-expression of miR-130b was evidently associated with advanced tumor stage (grade III+IV) in glioma. Moreover, we disclosed that the high-expression of miR-130b conferred an obviously reduced survival of glioma patients. Multivariate Cox regression analysis showed that miR-130b expression was an independent prognostic indicator for glioma patients. Our gain- or loss-of-function studies showed that miR-130b promoted invasion and migration of glioma cells. Notably, miR-130b regulated peroxisome proliferator-activated receptor gamma (PPARγ) abundance and epithelial-mesenchymal transition (EMT) in glioma cells. Hereby, PPARγ was identified as a functional target of miR-130b in glioma. Furthermore, an inverse correlation between miR-130b and PPARγ expression was observed in glioma tissues. In conclusion, miR-130b is an independent prognostic biomarker for indicating survival of glioma patients and promotes glioma cell migration and invasion by targeting PPARγ.
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Xi'an Medical College, No. 48 Fenghao West Road, Xi'an 710077, China.
authors.forename: Xudong
authors.initials: X
authors.lastname: Sheng
authors.affiliation: Department of Neurosurgery, Tang-Du Hospital, Fourth Military Medical University, No. 1 Xinsi Road, Xi'an 710038, China.
authors.forename: Hu
authors.initials: H
authors.lastname: Chen
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Xi'an Medical College, No. 48 Fenghao West Road, Xi'an 710077, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Xi'an Medical College, No. 48 Fenghao West Road, Xi'an 710077, China.
authors.forename: Zhibin
authors.initials: Z
authors.lastname: Ding
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Xi'an Medical College, No. 48 Fenghao West Road, Xi'an 710077, China.
authors.forename: Gangzhu
authors.initials: G
authors.lastname: Xu
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Xi'an Medical College, No. 48 Fenghao West Road, Xi'an 710077, China.
authors.forename: Junfeng
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Xi'an Medical College, No. 48 Fenghao West Road, Xi'an 710077, China.
authors.forename: Wenchao
authors.initials: W
authors.lastname: Lu
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Xi'an Medical College, No. 48 Fenghao West Road, Xi'an 710077, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Wu
authors.affiliation: Department of Anesthesiology, The First Affiliated Hospital of Xi'an Medical College, No. 48 Fenghao West Road, Xi'an 710077, China. Electronic address: jgsy_2014@sina.com.
authors.forename: Ling
authors.initials: L
authors.lastname: Zhao
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma / Q000235:genetics* / Q000473:pathology; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000235:genetics; D009367:Neoplasm Staging; D047495:PPAR gamma / Q000378:metabolism*; D011379:Prognosis; D016016:Proportional Hazards Models; D015854:Up-Regulation / Q000235:genetics
chemical_list: C554019:MIRN130 microRNA, human; D035683:MicroRNAs; D047495:PPAR gamma
keywords: Glioma; Invasion; MicroRNA-130b; Migration; Peroxisome proliferator-activated receptor gamma