title: [Effect of carbon disulfide exposure on fatty acid metabolism in ApoE knockout and C57BL/6J mice].
abstract: OBJECTIVE
  To study the influences of carbon disulfide (CS2) exposure on fatty acid metabolism in apolipoprotein E (ApoE) knockout mice and C57BL/6J mice.
  
  
  METHODS
  Twenty-four male ApoE knockout mice were randomly and equally divided into four groups: a CS2-exposed normal diet group, a CS2-unexposed normal diet group, a CS2-exposed high-fat diet group, and a CS2-unexposed high-fat diet group. Twenty-four C57BL/6J male mice were divided into four groups in the same way. The CS2-exposed groups were exposed to CS2 (1 g/m(3)) by static inhalation for 5 hours a day, 5 days a week. After two weeks, the whole blood of mice was collected. Methyl ester derivatization of fatty acids was performed using an acid-catalyzed method. Fatty acid contents before and after exposure were compared by gas chromatography-mass spectroscopy.
  
  
  RESULTS
  There were significant differences in fatty acid contents of mice between the four groups. For the C57BL/6J mice, the arachidic acid contents in the CS2-exposed high-fat diet group were significantly lower than those in the CS2-unexposed high-fat diet group (P = 0.045 0). For the ApoE knockout mice, the arachidonic acid contents in the CS2-exposed normal diet group were significantly lower than those in the CS2-unexposed control diet group (P = 0.045 2). For the ApoE knockout mice, the γ-linolenic acid contents in the CS2-exposed high-fat diet group were significantly higher than those in the unexposed high-fat diet group (P = 0.044 7).
  
  
  CONCLUSIONS
  Exposure to CS2 can induce fatty acid metabolism disorder in mice, indicating that CS2 may increase the risk of atherosclerosis and other cardiovascular diseases.
authors.affiliation: The School of Public Health, Nanjing Medical University, Nanjing 211166, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.forename: Shanlei
authors.initials: S
authors.lastname: Qiao
authors.forename: Lu
authors.initials: L
authors.lastname: Ding
authors.forename: Shouyu
authors.initials: S
authors.lastname: Wang
authors.forename: Jinglian
authors.initials: J
authors.lastname: Cao
authors.forename: Li
authors.initials: L
authors.lastname: Zhong
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Chunhui
authors.initials: C
authors.lastname: Ni
authors.affiliation: E-mail: zhubl@jscdc.cn.
authors.forename: Baoli
authors.initials: B
authors.lastname: Zhu
mesh_terms: D000280:Administration, Inhalation; D000818:Animals; D001057:Apolipoproteins E / Q000235:genetics; D050197:Atherosclerosis; D002246:Carbon Disulfide / Q000633:toxicity*; D059305:Diet, High-Fat; D005227:Fatty Acids / Q000737:chemistry; D050356:Lipid Metabolism / Q000187:drug effects*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout
chemical_list: D001057:Apolipoproteins E; D005227:Fatty Acids; D002246:Carbon Disulfide