title: Effect of spermidine and its metabolites on the activity of pea seedlings diamine oxidase and the problems of biosensing of biogenic amines with this enzyme.
abstract: Spermidine is one of the several biogenic amines, produced during the microbial decarboxylation of proteins. Individual biogenic amines in the formed mixtures are frequently analyzed with oxygen sensor based biosensors, as their content serves as a good biomarker for the determination of food quality. In these biosensors, diamine oxidase from pea seedlings (PSAO), catalyzing the oxidation of various biogenic amines by dissolved oxygen is commonly used for the bio-recognition of amines. However, in the presence of spermidine and/or its metabolite 1,3-diaminopropane, the activity of PSAO and the sensitivity of PSAO-based biosensors decrease due to inhibition. The inhibition constant of soluble spermidine, acting as an inhibiting substrate toward PSAO, was found to be (40±15) mM in freshly prepared solution and (0.28±0.05) mM in solution, incubated 30 days at room temperature. The inhibition constant of 1,3-diaminopropane, acting as a competitive inhibitor, was (0.43±0.12) mM as determined through the oxidation reaction of cadaverine. The metabolic half-life of soluble spermidine was 7 days at room temperature and 186 days at 4 °C. The kinetic measurements were carried out with an oxygen sensor; the composition of the solution of degraded spermidine was analyzed with MS.
authors.affiliation: Institute of Chemistry, University of Tartu, Ravila 14a, Tartu, Estonia.
authors.forename: K
authors.initials: K
authors.lastname: Kivirand
authors.affiliation: Institute of Chemistry, University of Tartu, Ravila 14a, Tartu, Estonia.
authors.forename: H
authors.initials: H
authors.lastname: Sõmerik
authors.affiliation: Institute of Chemistry, University of Tartu, Ravila 14a, Tartu, Estonia.
authors.forename: M-L
authors.initials: ML
authors.lastname: Oldekop
authors.affiliation: Institute of Chemistry, University of Tartu, Ravila 14a, Tartu, Estonia.
authors.forename: R
authors.initials: R
authors.lastname: Rebane
authors.affiliation: Institute of Chemistry, University of Tartu, Ravila 14a, Tartu, Estonia. Electronic address: toonika.rinken@ut.ee.
authors.forename: T
authors.initials: T
authors.lastname: Rinken
mesh_terms: D006631:Amine Oxidase (Copper-Containing) / Q000037:antagonists & inhibitors / Q000378:metabolism*; D001667:Binding, Competitive; D001679:Biogenic Amines / Q000032:analysis*; D015374:Biosensing Techniques; D002103:Cadaverine / Q000032:analysis / Q000378:metabolism; D003959:Diamines / Q000378:metabolism; D006207:Half-Life; D006632:Histamine / Q000032:analysis; D007700:Kinetics; D010100:Oxygen / Q000378:metabolism; D018532:Pisum sativum / Q000201:enzymology*; D010940:Plant Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D011700:Putrescine / Q000032:analysis; D036226:Seedlings / Q000201:enzymology; D021241:Spectrometry, Mass, Electrospray Ionization; D013095:Spermidine / Q000032:analysis / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D001679:Biogenic Amines; D003959:Diamines; D010940:Plant Proteins; D006632:Histamine; C009475:trimethylenediamine; D006631:Amine Oxidase (Copper-Containing); D002103:Cadaverine; D010100:Oxygen; D013095:Spermidine; D011700:Putrescine
keywords: 1,3-Diaminopropane; Biosensor; Diamine oxidase; Pea seedling; Spermidine biogenic amines