title: Pbx Regulates Patterning of the Cerebral Cortex in Progenitors and Postmitotic Neurons.
abstract: We demonstrate using conditional mutagenesis that Pbx1, with and without Pbx2(+/-) sensitization, regulates regional identity and laminar patterning of the developing mouse neocortex in cortical progenitors (Emx1-Cre) and in newly generated neurons (Nex1-Cre). Pbx1/2 mutants have three salient molecular phenotypes of cortical regional and laminar organization: hypoplasia of the frontal cortex, ventral expansion of the dorsomedial cortex, and ventral expansion of Reelin expression in the cortical plate of the frontal cortex, concomitant with an inversion of cortical layering in the rostral cortex. Molecular analyses, including PBX ChIP-seq, provide evidence that PBX promotes frontal cortex identity by repressing genes that promote dorsocaudal fate.
authors.affiliation: Department of Psychiatry, Neuroscience Program and the Nina Ireland Laboratory of Developmental Neurobiology, University of California San Francisco, San Francisco, CA 94158, USA; Acetylon Pharmaceuticals, 70 Fargo Street, Suite 205, Boston, MA 02210, USA. Electronic address: ogolonzhka@acetylon.com.
authors.forename: Olga
authors.initials: O
authors.lastname: Golonzhka
authors.affiliation: Departments of Neurobiology, Physiology, and Behavior and Psychiatry and Behavioral Sciences, Center for Neuroscience, University of California Davis, Davis, CA 95618, USA.
authors.forename: Alex
authors.initials: A
authors.lastname: Nord
authors.affiliation: Institute for Human Genetics, University of California San Francisco, 513 Parnassus Avenue, San Francisco, CA 94143, USA.
authors.forename: Paul L F
authors.initials: PLF
authors.lastname: Tang
authors.affiliation: Department of Psychiatry, Neuroscience Program and the Nina Ireland Laboratory of Developmental Neurobiology, University of California San Francisco, San Francisco, CA 94158, USA.
authors.forename: Susan
authors.initials: S
authors.lastname: Lindtner
authors.affiliation: Department of Psychiatry, Neuroscience Program and the Nina Ireland Laboratory of Developmental Neurobiology, University of California San Francisco, San Francisco, CA 94158, USA.
authors.forename: Athena R
authors.initials: AR
authors.lastname: Ypsilanti
authors.affiliation: Department of Cell and Developmental Biology, Weill Medical College of Cornell University, 1300 York Avenue, New York, NY 10021, USA; The Danish Stem Cell Center, University of Copenhagen, Blegdamsvej 3B, DK-2200 Copenhagen, Denmark.
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Ferretti
authors.affiliation: Genomics Division, MS 84-171, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA; U.S. Department of Energy Joint Genome Institute, Walnut Creek, CA 94598, USA; School of Natural Sciences, University of California, Merced, Merced, CA 95343, USA.
authors.forename: Axel
authors.initials: A
authors.lastname: Visel
authors.affiliation: Department of Cell and Developmental Biology, Weill Medical College of Cornell University, 1300 York Avenue, New York, NY 10021, USA.
authors.forename: Licia
authors.initials: L
authors.lastname: Selleri
authors.affiliation: Department of Psychiatry, Neuroscience Program and the Nina Ireland Laboratory of Developmental Neurobiology, University of California San Francisco, San Francisco, CA 94158, USA. Electronic address: john.rubenstein@ucsf.edu.
authors.forename: John L R
authors.initials: JLR
authors.lastname: Rubenstein
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002540:Cerebral Cortex / Q000196:embryology* / Q000254:growth & development*; D018398:Homeodomain Proteins / Q000502:physiology*; D051379:Mice; D008822:Mice, Transgenic; D008938:Mitosis / Q000502:physiology; D058953:Neural Stem Cells / Q000502:physiology*; D009474:Neurons / Q000502:physiology*; D000075062:Pre-B-Cell Leukemia Transcription Factor 1; D000091042:Reelin Protein; D013234:Stem Cells / Q000502:physiology; D014157:Transcription Factors / Q000502:physiology*
chemical_list: D018398:Homeodomain Proteins; C481797:Pbx1 protein, mouse; D000075062:Pre-B-Cell Leukemia Transcription Factor 1; D000091042:Reelin Protein; D014157:Transcription Factors; C000717127:RELN protein, human; C000717128:Reln protein, mouse