title: Antioxidant activity and protection against oxidative-induced damage of Acacia shaffneri and Acacia farnesiana pods extracts: in vitro and in vivo assays.
abstract: BACKGROUND
  Obesity is a worldwide public health issue, reaching epidemic condition in developing countries associated to chronic diseases. Oxidative damage is another side effect of obesity. Antioxidant activity from plant components regulates at some extent this imbalance. Main goal of the present study was to determine the antioxidant activity and protection against oxidative-induced damage of Acacia shaffneri (AS) and Acacia farnesiana (AF) pods extracts.
  
  
  METHODS
  To evaluated antioxidant activity and radical scavenging capacity of AS and AF extracts, two experiments were performed: 1) pods extracts were challenged against H2O2 using kidney cells in an in vitro assay; and 2) (Meriones unguiculatus) was employed in an in vivo assay to observe the effect of pods extracts on scavenging properties in plasma.
  
  
  RESULTS
  Both pods extracts presented an important protective effect on radical scavenging capacity against ABTS• + and DPPH(+), and also in TBARS formation in vitro. Vegetal pods extracts did not induce any pro-oxidative effect when added to kidney cells in DMEM. Cells damage in DMEM with addition of H2O2 was significantly higher than those when vegetal pods extracts were added at 50 (P < 0.05) or 200 ppm (P < 0.001). Plasma scavenging properties presented an important dose-dependent positive effect in those groups where pods extracts were administered.
  
  
  CONCLUSIONS
  The antioxidant protection of the acacia pods extracts reported in this study suggests the possible transference of antioxidant components and protective effects to animal products (milk, meat, and by-products) from Acacia pods when this vegetation is included in the diet. In order to evaluate, the possible transference of theirs antioxidant components to animal products, the incorporation of these non-conventional resources to ruminant feeding is a good opportunity of study. Profiling of Acacia farnesiana pods extract is necessary to identify the responsible bioactive compounds of protective properties.
authors.affiliation: National Institute of Medical Science and Nutrition "Salvador Zubirán", Vasco de Quiroga 15., Mexico City, 14000, Mexico. claudia.delgadillop@incmnsz.mx.
authors.forename: Claudia
authors.initials: C
authors.lastname: Delgadillo Puga
authors.affiliation: National Institute of Medical Science and Nutrition "Salvador Zubirán", Vasco de Quiroga 15., Mexico City, 14000, Mexico.
authors.forename: Mario
authors.initials: M
authors.lastname: Cuchillo Hilario
authors.affiliation: Faculty of Chemistry, National Autonomous University of Mexico, Mexico City, 04510, Mexico.
authors.forename: José Guillermo
authors.initials: JG
authors.lastname: Espinosa Mendoza
authors.affiliation: Department of Biology, Faculty of Chemistry, National Autonomous University of Mexico, Mexico City, 04510, Mexico.
authors.forename: Omar
authors.initials: O
authors.lastname: Medina Campos
authors.affiliation: Department of Biology, Faculty of Chemistry, National Autonomous University of Mexico, Mexico City, 04510, Mexico.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Molina Jijón
authors.affiliation: National Institute of Medical Science and Nutrition "Salvador Zubirán", Vasco de Quiroga 15., Mexico City, 14000, Mexico.
authors.forename: Margarita
authors.initials: M
authors.lastname: Díaz Martínez
authors.affiliation: National Institute of Medical Science and Nutrition "Salvador Zubirán", Vasco de Quiroga 15., Mexico City, 14000, Mexico.
authors.forename: Marsela Alejandra
authors.initials: MA
authors.lastname: Álvarez Izazaga
authors.affiliation: National Institute of Medical Science and Nutrition "Salvador Zubirán", Vasco de Quiroga 15., Mexico City, 14000, Mexico.
authors.forename: José Ángel
authors.initials: JÁ
authors.lastname: Ledesma Solano
authors.affiliation: Department of Biology, Faculty of Chemistry, National Autonomous University of Mexico, Mexico City, 04510, Mexico.
authors.forename: José
authors.initials: J
authors.lastname: Pedraza Chaverri
mesh_terms: D000045:Acacia; D000818:Animals; D000975:Antioxidants / Q000032:analysis / Q000494:pharmacology*; D002460:Cell Line / Q000187:drug effects; D045424:Complex Mixtures / Q000494:pharmacology*; D005260:Female; D016166:Free Radical Scavengers / Q000494:pharmacology; D005849:Gerbillinae; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D035261:Plant Components, Aerial; D010936:Plant Extracts / Q000494:pharmacology; D010949:Plasma / Q000737:chemistry*; D013552:Swine
chemical_list: D000975:Antioxidants; D045424:Complex Mixtures; D016166:Free Radical Scavengers; D010936:Plant Extracts