title: Effect of nonsurgical periodontal therapy on salivary myeloperoxidase levels: A biochemical study.
abstract: BACKGROUND
  Myeloperoxidase (MPO), the most abundant protein in neutrophils, is the focus of inflammatory pathologies. MPO could participate in the initiation and progression of periodontal disease.
  
  
  METHODS
  A total of 60 patients with healthy gingiva, gingivitis, periodontitis between age group of 20-55 years were selected. Group I - 20 Patients with healthy gingiva, Group II - 20 Patients with generalized gingivitis, Group III - 20 Patients with generalized chronic periodontitis, Group IV - 20 Patients of Group III after 1-month of scaling and root planning. The following parameters were recorded: Gingival index, plaque index, bleeding on probing index, probing pocket depth, clinical attachment level, salivary MPO levels. All the parameters were then statistically analyzed.
  
  
  RESULTS
  The mean MPO levels in Group I recorded was - 0.320 + 0.06, Group II was - 0.183 + 0.04, Group III was - 0.814 + 0.08 and Group IV was - 0.386 + 0.08 respectively. All these values were statistically significant when compared between the four groups (P < 0.05). A significantly elevated salivary MPO levels were found in subjects with chronic periodontitis as compared to the gingivitis group and the healthy group (P < 0.05). However, moderate but statistically significant increase in the MPO levels were observed in the gingivitis group as compared to the healthy group (P < 0.05). Furthermore, significant reduction in MPO levels were observed in Group IV after 1-month of nonsurgical periodontal therapy.
  
  
  CONCLUSIONS
  The activities of MPO enzyme were significantly increased in the saliva of patients with periodontal disease in comparison to healthy individuals. Furthermore, nonsurgical periodontal therapy was found to be effective in improving clinical parameters and in reducing MPO levels. Salivary enzymes like MPO could be considered as a biochemical marker of periodontal disease activity.
authors.affiliation: Department of Periodontology, Subharti Dental College and Hospital, Meerut, Uttar Pradesh, India.
authors.forename: Mona
authors.initials: M
authors.lastname: Dagar
authors.affiliation: Department of Periodontology, Subharti Dental College and Hospital, Meerut, Uttar Pradesh, India.
authors.forename: Dhruv Kumar
authors.initials: DK
authors.lastname: Deepa
authors.affiliation: Department of Microbiology, Subharti Dental College and Hospital, Meerut, Uttar Pradesh, India.
authors.forename: Madan
authors.initials: M
authors.lastname: Molly
authors.affiliation: Department of Periodontology, Subharti Dental College and Hospital, Meerut, Uttar Pradesh, India.
authors.forename: Anamika
authors.initials: A
authors.lastname: Sharma
authors.affiliation: Department of Periodontology, Subharti Dental College and Hospital, Meerut, Uttar Pradesh, India.
authors.forename: Braham Prakash
authors.initials: BP
authors.lastname: Khattak
keywords: Ghronic periodontitis; gingivitis; myeloperoxidase; nonsurgical periodontal therapy; periodontal disease; spectrophotometer