title: Correlation of ERCC1 expression in peripheral blood lymphocytes with outcomes of patients with gastric cancer treated with oxaliplatin-based adjuvant chemotherapy.
abstract: Excision repair cross-complementing gene-1 (ERCC1) is a key regulatory enzyme whose expression patterns in tumor tissues are associated with survival in gastric cancer. The present study aimed to evaluate the effects of ERCC1 expression in peripheral blood lymphocytes (PBLs) on the outcome of patients with gastric cancer treated with oxaliplatin-based adjuvant chemotherapy. Tumor and PBL samples from 48 patients treated with adjuvant oxaliplatin-based chemotherapy for gastric cancer were analyzed. Immunohistochemistry was used to assess the expression of ERCC1. After a median follow-up of 18.5 months, the median disease-free survival (DFS) and overall survival (OS) were 12 and 20 months, respectively. Expression of ERCC1 was found in 72.9% (35/48), 56.3% (27/48), and 10.0% (2/20) of tumor tissues, PBLs from gastric cancer patients, and PBLs from controls, respectively. A significant positive correlation between ERCC1 expression in PBL and cancer tissue was found (χ(2) = 12.098, P = 0.001, Pearson contingency coefficient = 0.502). Patients with negative expression of ERCC1 in tumor tissues had a significantly longer median DFS and median OS compared to patients with positive expression of ERCC1 (median DFS, 18 vs 10 months, P = 0.006; median OS, 30 vs 17 months, P = 0.012). In PBLs, high expression of ERCC1 was associated with decreased DFS (9 vs 18 months, P = 0.032), but not OS (16 vs 24 months, P = 0.057). Patients with gastric cancer exhibiting negative expression of ERCC1 are more likely to benefit from oxaliplatin-based adjuvant chemotherapy.
authors.affiliation: Department of Oncology, First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China.
authors.forename: Y Y
authors.initials: YY
authors.lastname: Zhang
authors.affiliation: Department of Oncology, First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China.
authors.forename: K S
authors.initials: KS
authors.lastname: Gu
authors.affiliation: Department of Oncology, First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China.
authors.forename: H Y
authors.initials: HY
authors.lastname: Wu
authors.affiliation: Department of Pathology, Basic Medicine College of Anhui Medical University, Hefei, Anhui, China.
authors.forename: F
authors.initials: F
authors.lastname: Yang
authors.affiliation: Department of Oncology, First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China.
authors.forename: L J
authors.initials: LJ
authors.lastname: Bu
authors.affiliation: Department of Oncology, First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China.
authors.forename: C C
authors.initials: CC
authors.lastname: Zhao
authors.affiliation: Department of Oncology, First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China.
authors.forename: Y R
authors.initials: YR
authors.lastname: Zhang
mesh_terms: D000328:Adult; D000368:Aged; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000627:therapeutic use; D017024:Chemotherapy, Adjuvant; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D004720:Endonucleases / Q000235:genetics / Q000378:metabolism*; D005260:Female; D005500:Follow-Up Studies; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008214:Lymphocytes / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging; D009944:Organoplatinum Compounds / Q000008:administration & dosage; D000077150:Oxaliplatin; D011379:Prognosis; D013274:Stomach Neoplasms / Q000188:drug therapy / Q000378:metabolism* / Q000401:mortality* / Q000473:pathology; D016896:Treatment Outcome; D055815:Young Adult
chemical_list: D004268:DNA-Binding Proteins; D009944:Organoplatinum Compounds; D000077150:Oxaliplatin; C071447:ERCC1 protein, human; D004720:Endonucleases