title: Upregulation of PTEN suppresses invasion in Tca8113 tongue cancer cells through repression of epithelial-mesenchymal transition (EMT).
abstract: We previously discovered that the expression of the tumor suppressor phosphatase and tensin homolog (PTEN) was downregulated in the majority patients with tongue squamous cell carcinoma (TSCC). The aim of this study was to investigate the role of PTEN overexpression in the regulation of epithelial-mesenchymal transition (EMT) of the tongue squamous carcinoma cell line Tca8113 as well as explore the underlying mechanism. GV230 (containing the PTEN gene) and empty vectors were transfected into Tca8113 cells. After stable transfection, the messenger RNA (mRNA) and protein levels of PTEN were validated using quantitative real-time PCR (qPCR) and Western blot analysis. The growth and cell cycle were analyzed using Cell Counting Kit-8 (CCK-8) and flow cytometry, respectively. The invasion ability was measured with a transwell assay. The effects of PTEN overexpression on EMT and Hedgehog signaling were assessed by comparing Tca8113-PTEN cells with control and negative control cell groups. We found that PTEN expression was significantly upregulated after transfection. Meanwhile, upregulated PTEN inhibited the proliferation and invasion of Tca8113 cells. In addition, we observed changes in the EMT- and Hedgehog-associated proteins. These data demonstrated that PTEN upregulation could reduce invasion by inhibiting the process of EMT in Tca8113 cells, which might be related to the Hedgehog signaling pathway.
authors.affiliation: Department of Stomatology, School of Medicine, Jinan University, Guangzhou, 510632, China.
authors.forename: Siming
authors.initials: S
authors.lastname: Xie
authors.affiliation: Department of Stomatology, School of Medicine, Jinan University, Guangzhou, 510632, China.
authors.forename: Zhiyuan
authors.initials: Z
authors.lastname: Lu
authors.affiliation: Department of Stomatology, School of Medicine, Jinan University, Guangzhou, 510632, China.
authors.forename: Yanzhu
authors.initials: Y
authors.lastname: Lin
authors.affiliation: Department of Stomatology, School of Medicine, Jinan University, Guangzhou, 510632, China.
authors.forename: Lijia
authors.initials: L
authors.lastname: Shen
authors.affiliation: Department of Oral Medicine, Guangdong Provincial Stomatological Hospital, Guangzhou, 510280, China. yin9731@sina.com.
authors.forename: Cao
authors.initials: C
authors.lastname: Yin
mesh_terms: D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D053823:Hedgehog Proteins / Q000378:metabolism; D006801:Humans; D051059:PTEN Phosphohydrolase / Q000235:genetics*; D015398:Signal Transduction; D014062:Tongue Neoplasms / Q000209:etiology / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*
chemical_list: D053823:Hedgehog Proteins; D051059:PTEN Phosphohydrolase
keywords: EMT; Invasion; PTEN; Tongue squamous cell carcinoma