title: Development of prilling process for biodegradable microspheres through experimental designs.
abstract: The prilling process proposes a microparticle formulation easily transferable to the pharmaceutical production, leading to monodispersed and highly controllable microspheres. PLGA microspheres were used for carrying an encapsulated protein and adhered stem cells on its surface, proposing a tool for regeneration therapy against injured tissue. This work focused on the development of the production of PLGA microspheres by the prilling process without toxic solvent. The required production quality needed a complete optimization of the process. Seventeen parameters were studied through experimental designs and led to an acceptable production. The key parameters and mechanisms of formation were highlighted.
authors.affiliation: LUNAM Université, Micro et Nanomédecines Biomimétiques (MINT), F-49933 Angers, France; INSERM U1066, F-49933 Angers, France.
authors.forename: Violet
authors.initials: V
authors.lastname: Fabien
authors.affiliation: LUNAM Université, Micro et Nanomédecines Biomimétiques (MINT), F-49933 Angers, France; INSERM U1066, F-49933 Angers, France.
authors.forename: Le
authors.initials: L
authors.lastname: Minh-Quan
authors.affiliation: Aix Marseille Université, LISA, EA 4672, 13013 Marseille, France.
authors.forename: Sergent
authors.initials: S
authors.lastname: Michelle
authors.affiliation: LUNAM Université, Micro et Nanomédecines Biomimétiques (MINT), F-49933 Angers, France; INSERM U1066, F-49933 Angers, France.
authors.forename: Bastiat
authors.initials: B
authors.lastname: Guillaume
authors.affiliation: LUNAM Université, Micro et Nanomédecines Biomimétiques (MINT), F-49933 Angers, France; INSERM U1066, F-49933 Angers, France.
authors.forename: Tran
authors.initials: T
authors.lastname: Van-Thanh
authors.affiliation: LUNAM Université, Micro et Nanomédecines Biomimétiques (MINT), F-49933 Angers, France; INSERM U1066, F-49933 Angers, France. Electronic address: venier@univ-angers.fr.
authors.forename: Venier-Julienne
authors.initials: VJ
authors.lastname: Marie-Claire
mesh_terms: D000818:Animals; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D002645:Chickens; D015195:Drug Design; D019344:Lactic Acid / Q000138:chemical synthesis* / Q000493:pharmacokinetics; D008837:Micrococcus / Q000378:metabolism; D008863:Microspheres; D009113:Muramidase / Q000737:chemistry* / Q000493:pharmacokinetics; D010316:Particle Size; D011092:Polyethylene Glycols / Q000138:chemical synthesis* / Q000493:pharmacokinetics; D011100:Polyglycolic Acid / Q000138:chemical synthesis* / Q000493:pharmacokinetics; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer
chemical_list: D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D011092:Polyethylene Glycols; D009113:Muramidase; C018557:glycofurol
keywords: Experimental design; Glycofurol; Microsphere; PLGA; Prilling