title: Meniscal allograft transplantation in rabbit.
abstract: This study evaluated the technique for meniscal allograft transplantation using allografts preserved in glycerin 98% in rabbits. Euthanasia was performed at 70 days to compare the transplanted (TM1 to TM16) versus the contralateral meniscus (OM1 to OM16). Sixteen menisci, 8 transplanted and 8 contralateral, were submitted to gross examination, histomorphometric analysis for identification and quantification of cellular type, and for quantification and distribution of collagen fibers. A revascularization study was conducted in all of the other samples. Lengths of the OM varied from 0.9 to 1.0 cm and two TM were smaller. All TM were completely attached to the synovial membrane, except for one case that presented partial fixation. Both, TM and OM had similar amounts of chondrocytes, fibroblasts and fibrocytes, and at the horns, chondrocytes were predominant. The collagen fibers in TM were well organized throughout the body, and disorganized at the horns. These fibers in OM were organized. The amounts of collagen type I and III, and the vascularization of the perimeniscal tissue and of the edge were similar in OM and TM. These results demonstrated graft integration and thus this transplantation technique and preservation method may be recommended.
authors.affiliation: Departamento de Veterinária, Universidade Federal de Viçosa, Viçosa, MG, Brasil.
authors.forename: Liana M
authors.initials: LM
authors.lastname: Vilela
authors.affiliation: Departamento de Veterinária, Universidade Federal de Viçosa, Viçosa, MG, Brasil.
authors.forename: Ricardo J
authors.initials: RJ
authors.lastname: Del Carlo
authors.affiliation: Departamento de Veterinária, Universidade Federal de Viçosa, Viçosa, MG, Brasil.
authors.forename: Edson V
authors.initials: EV
authors.lastname: Melo Filho
authors.affiliation: Departamento de Veterinária, Universidade Federal de Viçosa, Viçosa, MG, Brasil.
authors.forename: Lukiya S C
authors.initials: LS
authors.lastname: Favarato
authors.affiliation: Departamento de Veterinária, Universidade Federal de Viçosa, Viçosa, MG, Brasil.
authors.forename: Tatiana S
authors.initials: TS
authors.lastname: Duarte
authors.affiliation: Departamento de Veterinária, Universidade Federal de Viçosa, Viçosa, MG, Brasil.
authors.forename: Kelly C S
authors.initials: KC
authors.lastname: Pontes
authors.affiliation: Departamento de Veterinária, Universidade Federal de Viçosa, Viçosa, MG, Brasil.
authors.forename: Daise N Q
authors.initials: DN
authors.lastname: Cunha
mesh_terms: D000818:Animals; D001861:Bone Regeneration; D008297:Male; D008592:Menisci, Tibial / Q000637:transplantation*; D018919:Neovascularization, Physiologic; D011817:Rabbits; D014021:Tissue Preservation; D014184:Transplantation, Homologous