title: Synthesis and characterization of a multimode stationary phase: Congo red derivatized silica in nano-flow HPLC.
abstract: A novel Congo red (CR) derivatized silica stationary phase was prepared and packed into a fused silica capillary tube for nano-flow HPLC. A variety of analytes including poly-aromatic hydrocarbons, parabens, acids, sulfonamides, bases, and nucleosides were successfully separated using the CR. In comparison with commercial ODS columns, this new stationary phase has a different separation mechanism (hydrophobically-assisted ion-exchange), which was evident in the separation of benzoic acid derivatives and sulfonamides. The successful application of CR-bonded silica stationary phase in the HILIC and PALC modes demonstrates the effectiveness of this potential chromatographic material in nano flow HPLC.
authors.affiliation: School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241, P.R. China. qjwang@chem.ecnu.edu.cn.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Guan
authors.initials: G
authors.lastname: Wang
authors.forename: Wujuan
authors.initials: W
authors.lastname: Chen
authors.forename: Pingang
authors.initials: P
authors.lastname: He
authors.forename: Qingjiang
authors.initials: Q
authors.lastname: Wang
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase; D003224:Congo Red / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D007474:Ion Exchange; D036103:Nanotechnology / Q000379:methods*; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D003224:Congo Red; D012822:Silicon Dioxide