title: In vitro probiotic profiling of novel Enterococcus faecium and Leuconostoc mesenteroides from Tunisian freshwater fishes.
abstract: Novel lactic acid bacteria isolated from different organs of freshwater fish were examined for their potential application as probiotics in raw and processed foods. Four isolates of Enterococcus faecium and Leuconostoc mesenteroides were identified at the molecular level by 16S rRNA sequencing and random amplification of polymorphic DNA - polymerase chain reaction, and their antimicrobial activity against a panel of pathogens and food-poisoning bacteria was investigated. The whole bacteriocins of the 4 isolates were characterized by enterobacterial repetitive intergenic consensus sequences in PCR. The isolates exhibited high inhibitory activities against food-borne pathogens and spoilage microbial species and have significant probiotic profiles, since they survived at pH 3.0 and in the presence of bile salts, pancreatin, and pepsin, without any detectable hemolytic activity. Further, moderate heat resistance, adhesion ability to steel surfaces, and sensitivity to clinically relevant antimicrobial agents were revealed for all the isolates. These results highlight the specific probiotic properties of the strains and give evidence for potential application in minimally processed foods subjected to moderate heat processing.
authors.affiliation: a Laboratory of Microbiology and Pathology of Aquatic Organisms, Institut national des sciences et technologies de la mer (INSTM), rue 2 mars 1934, 2025 Salammbô, Tunisia.|b Laboratory of Venoms and Therapeutic Molecules, Pasteur Institute of Tunisia, University of Tunis El Manar, 13 place Pasteur, B.P. 74, 1002 Tunis, Tunisia.
authors.forename: Rim
authors.initials: R
authors.lastname: El-Jeni
authors.affiliation: a Laboratory of Microbiology and Pathology of Aquatic Organisms, Institut national des sciences et technologies de la mer (INSTM), rue 2 mars 1934, 2025 Salammbô, Tunisia.
authors.forename: Monia
authors.initials: M
authors.lastname: El Bour
authors.affiliation: c Laboratory of Food Technology, LHICA, Department of Analytical Chemistry, Nutrition and Food Science, University of Santiago de Compostela, Lugo, Spain.
authors.forename: Pilar
authors.initials: P
authors.lastname: Calo-Mata
authors.affiliation: c Laboratory of Food Technology, LHICA, Department of Analytical Chemistry, Nutrition and Food Science, University of Santiago de Compostela, Lugo, Spain.
authors.forename: Karola
authors.initials: K
authors.lastname: Böhme
authors.affiliation: c Laboratory of Food Technology, LHICA, Department of Analytical Chemistry, Nutrition and Food Science, University of Santiago de Compostela, Lugo, Spain.
authors.forename: Inmaculada C
authors.initials: IC
authors.lastname: Fernández-No
authors.affiliation: c Laboratory of Food Technology, LHICA, Department of Analytical Chemistry, Nutrition and Food Science, University of Santiago de Compostela, Lugo, Spain.
authors.forename: Jorge
authors.initials: J
authors.lastname: Barros-Velázquez
authors.affiliation: b Laboratory of Venoms and Therapeutic Molecules, Pasteur Institute of Tunisia, University of Tunis El Manar, 13 place Pasteur, B.P. 74, 1002 Tunis, Tunisia.|d Medical School of Tunis, University of Tunis El Manar, 15 rue Djebel Lakhdhar, La Rabta, 1007 Tunis, Tunisia.
authors.forename: Balkiss
authors.initials: B
authors.lastname: Bouhaouala-Zahar
mesh_terms: D000818:Animals; D001430:Bacteriocins / Q000032:analysis; D016984:Enterococcus faecium; D005399:Fishes / Q000382:microbiology*; D005618:Fresh Water; D006801:Humans; D007934:Leuconostoc; D019936:Probiotics / Q000494:pharmacology*
chemical_list: D001430:Bacteriocins
keywords: Enterococcus; Leconostoc; Leuconostoc; aliments transformés; bacteriocins; bactériocines; bioconservation des aliments; enterocins; entérocines; food biopreservation; freshwater fish; poissons d’eau douce; probiotics; probiotiques; processed foods