title: In Vitro Fertilization, Levels of Pro-Inflammatory Factors and Lipid Peroxidation.
abstract: BACKGROUND
  Infertility is a problem concerning 10-15% of the individuals in the fertile period. This study investigated effects of proinflammatory factors as well as lipid hydroperoxides (LPO) levels upon in vitro fertilization (IVF) success.
  
  
  METHODS
  In this prospective, non-randomized, controlled clinical study, sera obtained from 26 fertile (group-1), 26 infertile women before (group-2) and after (group-3) IVF treatment were analyzed. Leptin, leptin receptor, resistin, tumor necrosis factor-alpha (TNF-α), and C-reactive protein (CRP) were analyzed using enzyme-linked immunosorbent assay (ELISA). LPO was determined spectrophotometrically. Mann- Whitney U test, paired samples t test, Wilcoxon signed-rank test as well as Pearson correlation analysis by SPSS were performed for statistical analysis.
  
  
  RESULTS
  TNF-α, resistin and LPO levels increased (P=0.020, P=0.003, P=0.001, respectively) in group-3 compared to group-2. A significant increase in LPO was noted both in group-2 and -3 compared to controls (P=0.000). LPO were higher in non-pregnants than pregnants in group-2. For pregnants, significant correlations were observed between leptin and resistin in group-2 and TNF-α and leptin in group-3. None of these correlations were found for the women, who could not conceive.
  
  
  CONCLUSIONS
  LPO, leptin-resistin correlation, associations with TNF-α may be helpful during the interpretation of IVF success rates.
authors.affiliation: Cerrahpasa Medical Faculty, Istanbul University, Istanbul, Turkey.
authors.forename: Ferdag
authors.initials: F
authors.lastname: Yildizfer
authors.affiliation: Cerrahpasa Medical Faculty, Istanbul University, Istanbul, Turkey.
authors.forename: Orkide
authors.initials: O
authors.lastname: Donma
authors.affiliation: Cerrahpasa Medical Faculty, Istanbul University, Istanbul, Turkey.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Yen
authors.affiliation: Cerrahpasa Medical Faculty, Istanbul University, Istanbul, Turkey.
authors.forename: Ozlem
authors.initials: O
authors.lastname: Ekmekci
authors.affiliation: Ministry of Health, Diyarbakir Education and Research Hospital, Diyarbakir, Turkey.
authors.forename: Zubeyde Asli
authors.initials: ZA
authors.lastname: Karatas Kul
authors.affiliation: University of Texas, Health Science Center at Houston, Houston, USA.
authors.forename: Zafer
authors.initials: Z
authors.lastname: Keser
authors.affiliation: Tufts Medical Center, Molecular Oncology Research Institute, Boston, USA.
authors.forename: Ahmet
authors.initials: A
authors.lastname: Esat Imal
authors.affiliation: Cerrahpasa Medical Faculty, Istanbul University, Istanbul, Turkey.
authors.forename: Emin
authors.initials: E
authors.lastname: Cagil
authors.affiliation: Cerrahpasa Medical Faculty, Istanbul University, Istanbul, Turkey.
authors.forename: Murat
authors.initials: M
authors.lastname: Mengi
authors.affiliation: Cerrahpasa Medical Faculty, Istanbul University, Istanbul, Turkey.
authors.forename: Hakan
authors.initials: H
authors.lastname: Ekmekci
authors.affiliation: Cerrahpasa Medical Faculty, Istanbul University, Istanbul, Turkey.
authors.forename: Sezai
authors.initials: S
authors.lastname: Sahmay
authors.affiliation: Faculty of Medicine, Namik Kemal University, Tekirdag, Turkey.
authors.forename: Burcin
authors.initials: B
authors.lastname: Nalbantoglu
authors.affiliation: Faculty of Medicine, Namik Kemal University, Tekirdag, Turkey.
authors.forename: Mustafa Metin
authors.initials: MM
authors.lastname: Donma
keywords: Infertility; Leptin; Lipid Peroxidation; Resistin; TNF-α