title: A Panel of Protease-Responsive RNA Polymerases Respond to Biochemical Signals by Production of Defined RNA Outputs in Live Cells.
abstract: RNA is an attractive biomolecule for biosensing and engineering applications due to its information storage capacity and ability to drive gene expression or knockdown. However, methods to link chemical signals to the production of specific RNAs are lacking. Here, we develop protease-responsive RNA polymerases (PRs) as a strategy to encode multiple specific proteolytic events in defined sequences of RNA in live mammalian cells. This work demonstrates that RNAP-based molecular recording devices can be deployed for multimodal analyses of biochemical activities or to trigger gene circuits using measured signaling events.
authors.affiliation: Department of Chemistry, The University of Chicago , 5801 South Ellis Avenue, Chicago, Illinois 60637, United States.
authors.forename: Jinyue
authors.initials: J
authors.lastname: Pu
authors.affiliation: Department of Chemistry, The University of Chicago , 5801 South Ellis Avenue, Chicago, Illinois 60637, United States.
authors.forename: Ian
authors.initials: I
authors.lastname: Chronis
authors.affiliation: Department of Chemistry, The University of Chicago , 5801 South Ellis Avenue, Chicago, Illinois 60637, United States.
authors.forename: Daniel
authors.initials: D
authors.lastname: Ahn
authors.affiliation: Department of Chemistry, The University of Chicago , 5801 South Ellis Avenue, Chicago, Illinois 60637, United States.
authors.forename: Bryan C
authors.initials: BC
authors.lastname: Dickinson
mesh_terms: D000818:Animals; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D008854:Microscopy, Electron; D010447:Peptide Hydrolases / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D012321:DNA-Directed RNA Polymerases; D010447:Peptide Hydrolases