title: Downscaling the in vitro test of fungal bioremediation of polycyclic aromatic hydrocarbons: methodological approach.
abstract: The miniaturization and optimization of a white rot fungal bioremediation experiment is described in this paper. The optimized procedure allows determination of the degradation kinetics of anthracene. The miniaturized procedure requires only 2.5 ml of culture medium. The experiment is more precise, robust, and better controlled comparing it to classical tests in flasks. Using this technique, different parts, i.e., the culture medium, the fungi, and the cotton seal, can be analyzed. A simple sample preparation speeds up the analytical process. Experiments performed show degradation of anthracene up to approximately 60% by Irpex lacteus and up to approximately 40% by Pleurotus ostreatus in 25 days. Bioremediation of anthracene by the consortium of I. lacteus and P. ostreatus shows the biodegradation of anthracene up to approximately 56% in 23 days. At the end of the experiment, the surface tension of culture medium decreased comparing it to the blank, indicating generation of surfactant compounds.
authors.affiliation: Department of Biology/Environmental Research Centre, Faculty of Natural Sciences, Vytautas Magnus University, Vileikos St. 8, 40444, Kaunas, Lithuania.
authors.forename: Tomas
authors.initials: T
authors.lastname: Drevinskas
authors.affiliation: Department of Biology/Environmental Research Centre, Faculty of Natural Sciences, Vytautas Magnus University, Vileikos St. 8, 40444, Kaunas, Lithuania.
authors.forename: Rūta
authors.initials: R
authors.lastname: Mickienė
authors.affiliation: Department of Biology/Environmental Research Centre, Faculty of Natural Sciences, Vytautas Magnus University, Vileikos St. 8, 40444, Kaunas, Lithuania. a.maruska@gmf.vdu.lt.
authors.forename: Audrius
authors.initials: A
authors.lastname: Maruška
authors.affiliation: Department of Biology/Environmental Research Centre, Faculty of Natural Sciences, Vytautas Magnus University, Vileikos St. 8, 40444, Kaunas, Lithuania.
authors.forename: Mantas
authors.initials: M
authors.lastname: Stankevičius
authors.affiliation: Department of Biology/Environmental Research Centre, Faculty of Natural Sciences, Vytautas Magnus University, Vileikos St. 8, 40444, Kaunas, Lithuania.
authors.forename: Nicola
authors.initials: N
authors.lastname: Tiso
authors.affiliation: Department of Biology/Environmental Research Centre, Faculty of Natural Sciences, Vytautas Magnus University, Vileikos St. 8, 40444, Kaunas, Lithuania.
authors.forename: Jurgita
authors.initials: J
authors.lastname: Mikašauskaitė
authors.affiliation: Kaunas Botanical Garden of Vytautas Magnus University, Ž. E. Žilibero 6, 46324, Kaunas, Lithuania.
authors.forename: Ona
authors.initials: O
authors.lastname: Ragažinskienė
authors.affiliation: Process Control Department, Kaunas University of Technology, Studentų St. 50, 51368, Kaunas, Lithuania.
authors.forename: Donatas
authors.initials: D
authors.lastname: Levišauskas
authors.affiliation: Department of Biology/Environmental Research Centre, Faculty of Natural Sciences, Vytautas Magnus University, Vileikos St. 8, 40444, Kaunas, Lithuania.
authors.forename: Violeta
authors.initials: V
authors.lastname: Bartkuvienė
authors.affiliation: Kaunas Botanical Garden of Vytautas Magnus University, Ž. E. Žilibero 6, 46324, Kaunas, Lithuania.
authors.forename: Vilija
authors.initials: V
authors.lastname: Snieškienė
authors.affiliation: Kaunas Botanical Garden of Vytautas Magnus University, Ž. E. Žilibero 6, 46324, Kaunas, Lithuania.
authors.forename: Antanina
authors.initials: A
authors.lastname: Stankevičienė
authors.affiliation: National Research Council, Institute of Agro-Environmental and Forest Biology & Institute of Chemical Methodologies, Area della Ricerca di Roma, via Salaria Km 29.300, 00015, Monterotondo, Rome, Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: Polcaro
authors.affiliation: National Research Council, Institute of Agro-Environmental and Forest Biology & Institute of Chemical Methodologies, Area della Ricerca di Roma, via Salaria Km 29.300, 00015, Monterotondo, Rome, Italy.
authors.forename: Emanuela
authors.initials: E
authors.lastname: Galli
authors.affiliation: National Research Council, Institute of Agro-Environmental and Forest Biology & Institute of Chemical Methodologies, Area della Ricerca di Roma, via Salaria Km 29.300, 00015, Monterotondo, Rome, Italy.
authors.forename: Enrica
authors.initials: E
authors.lastname: Donati
authors.affiliation: Process Control Department, Kaunas University of Technology, Studentų St. 50, 51368, Kaunas, Lithuania.
authors.forename: Tomas
authors.initials: T
authors.lastname: Tekorius
authors.affiliation: Department of Biology/Environmental Research Centre, Faculty of Natural Sciences, Vytautas Magnus University, Vileikos St. 8, 40444, Kaunas, Lithuania.
authors.forename: Olga
authors.initials: O
authors.lastname: Kornyšova
authors.affiliation: Department of Biology/Environmental Research Centre, Faculty of Natural Sciences, Vytautas Magnus University, Vileikos St. 8, 40444, Kaunas, Lithuania.
authors.forename: Vilma
authors.initials: V
authors.lastname: Kaškonienė
mesh_terms: D000873:Anthracenes / Q000378:metabolism; D001487:Basidiomycota / Q000378:metabolism; D003470:Culture Media / Q000378:metabolism; D004785:Environmental Pollutants / Q000378:metabolism; D052918:Environmental Restoration and Remediation / Q000379:methods*; D005658:Fungi / Q000378:metabolism; D066298:In Vitro Techniques; D057230:Limit of Detection; D009281:Naphthalenes / Q000378:metabolism; D020076:Pleurotus / Q000378:metabolism*; D011084:Polycyclic Aromatic Hydrocarbons / Q000378:metabolism*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000873:Anthracenes; D003470:Culture Media; D004785:Environmental Pollutants; D009281:Naphthalenes; D011084:Polycyclic Aromatic Hydrocarbons; C031721:naphthalene; C034020:anthracene
keywords: Bioremediation; Miniaturization; PAH; UPLC; White rot fungi