title: Development and characterization of monoclonal antibodies against goose CD3ɛ extracellular domain and their application in detection of CD3(+) T lymphocytes.
abstract: CD3 is one of the most important cell surface markers of T lymphocytes, and has an important function in signal transmission during antigen recognition. In this study, monoclonal antibodies against goose CD3ɛ extracellular domain were developed and characterized for the first time. The purified recombinant goose CD3ɛ extracellular domain protein and pcDNA3.1-GoCD3ɛex were used as immunogens to develop mAbs. Furthermore, recombinant protein was used to screen for mAbs. The characteristics of mAbs were identified through IFA, I-ELISA, Western blot, FCM, and LSCM analyzes. Results showed that mAbs 3C11 and 5A3 may be good candidates for use in detecting goose T lymphocytes and in immunoassaying and exploring the function of the CD3ɛ molecule.
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Xuelian
authors.initials: X
authors.lastname: Zhang
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Jianwei
authors.initials: J
authors.lastname: Shao
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Chunqiu
authors.initials: C
authors.lastname: Cao
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China; College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China. Electronic address: mabo99@neau.edu.cn.
authors.forename: Bo
authors.initials: B
authors.lastname: Ma
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China. Electronic address: jwwang@neau.edu.cn.
authors.forename: Junwei
authors.initials: J
authors.lastname: Wang
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D030161:Avian Proteins / Q000737:chemistry / Q000378:metabolism; D017252:CD3 Complex / Q000378:metabolism*; D005434:Flow Cytometry; D005777:Geese / Q000276:immunology* / Q000378:metabolism; D005786:Gene Expression Regulation / Q000276:immunology; D007118:Immunoassay; D051379:Mice; D008807:Mice, Inbred BALB C; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D030161:Avian Proteins; D017252:CD3 Complex; D011994:Recombinant Proteins
keywords: Application; Characterization; Goose CD3ɛ; Monoclonal antibodies