title: iTAP: integrated transcriptomics and phenotype database for stress response of Escherichia coli and Saccharomyces cerevisiae.
abstract: BACKGROUND
  Organisms are subject to various stress conditions, which affect both the organism's gene expression and phenotype. It is critical to understand microbial responses to stress conditions and uncover the underlying molecular mechanisms. To this end, it is necessary to build a database that collects transcriptomics and phenotypic data of microbes growing under various stress factors for in-depth systems biology analysis. Despite of numerous databases that collect gene expression profiles, to our best knowledge, there are few, if any, databases that collect both transcriptomics and phenotype data simultaneously. In light of this, we have developed an open source, web-based database, namely integrated transcriptomics and phenotype (iTAP) database, that records and links the transcriptomics and phenotype data for two model microorganisms, Escherichia coli and Saccharomyces cerevisiae in response to exposure of various stress conditions.
  
  
  RESULTS
  To collect the data, we chose relevant research papers from the PubMed database containing all the necessary information for data curation including experimental conditions, transcriptomics data, and phenotype data. The transcriptomics data, including the p value and fold change, were obtained through the comparison of test strains against control strains using Gene Expression Omnibus's GEO2R analyzer. The phenotype data, including the cell growth rate and the productivity, volumetric rate, and mass-based yield of byproducts, were calculated independently from charts or graphs within the reference papers. Since the phenotype data was never reported in a standardized format, the curation of correlated transcriptomics-phenotype datasets became extremely tedious and time-consuming. Despite the challenges, till now, we successfully correlated 57 and 143 datasets of transcriptomics and phenotype for E. coli and S. cerevisiae, respectively, and applied a regression model within the iTAP database to accurately predict over 93 and 73 % of the growth rates of E. coli and S. cerevisiae, respectively, directly from the transcriptomics data.
  
  
  CONCLUSIONS
  This is the first time that transcriptomics and phenotype data are categorized and correlated in an open-source database. This allows biologists to access the database and utilize it to predict the phenotype of microorganisms from their transcriptomics data. The iTAP database is freely available at https://sites.google.com/a/vt.edu/biomolecular-engineering-lab/software .
authors.affiliation: Department of Biological Systems Engineering, Virginia Tech, Blacksburg, VA, 24061, USA. sniveda5@vt.edu.
authors.forename: Niveda
authors.initials: N
authors.lastname: Sundararaman
authors.affiliation: Department of Biological Systems Engineering, Virginia Tech, Blacksburg, VA, 24061, USA. chrisa9@vt.edu.
authors.forename: Christine
authors.initials: C
authors.lastname: Ash
authors.affiliation: Department of Biological Systems Engineering, Virginia Tech, Blacksburg, VA, 24061, USA. gweihua2@vt.edu.
authors.forename: Weihua
authors.initials: W
authors.lastname: Guo
authors.affiliation: Commonwealth Governor's School, Fredericksburg, VA, 22407, USA. einsteinunicorn@gmail.com.
authors.forename: Rebecca
authors.initials: R
authors.lastname: Button
authors.affiliation: Department of Biological Systems Engineering, Virginia Tech, Blacksburg, VA, 24061, USA. jsingh16@vt.edu.
authors.forename: Jugroop
authors.initials: J
authors.lastname: Singh
authors.affiliation: Department of Biological Systems Engineering, Virginia Tech, Blacksburg, VA, 24061, USA. xueyang@vt.edu.
authors.forename: Xueyang
authors.initials: X
authors.lastname: Feng
mesh_terms: D019295:Computational Biology / Q000379:methods*; D030541:Databases, Genetic; D004926:Escherichia coli / Q000235:genetics* / Q000502:physiology; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D015966:Gene Expression Regulation, Fungal; D020407:Internet; D010641:Phenotype; D015203:Reproducibility of Results; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000502:physiology; D059467:Transcriptome / Q000235:genetics*