title: In Vitro Propagation, Phytochemical Analysis, and Evaluation of Free Radical Scavenging Property of Scrophularia kakudensis Franch Tissue Extracts.
abstract: The current study deals with in vitro propagation, antioxidant property estimation, and assessment of acacetin content in Scrophularia kakudensis Franch. Adventitious shoot induction was achieved from the nodal explant with the highest number of adventitious shoots per explant (17.4) on Murashige and Skoog's (MS) medium fortified with 2.0 mg·L(-1) 6-benzyladenine (BA) and 0.5 mg L(-1) indole-3-acetic acid (IAA). Maximum number of roots per plant (16.5) was noted in half strength MS medium supplemented with 0.5 mg·L(-1) IAA. The regenerated plants displayed successful survival ratio (95%) in the greenhouse. The highest content of acacetin, a pharmaceutically important flavonoid, was observed in the shoot extracts (in vitro: 32.83 µg·g(-1) FW; in vivo: 30.05 µg·g(-1) FW) followed by root extracts. Total phenol and flavonoid contents along with free radical scavenging assays revealed the occurrence of larger amount of antioxidants in shoot extract in comparison with callus and root extracts of S. kakudensis. Thus, the outcome of the present study can be highly beneficial for the germplasm conservation and commercial cultivation of S. kakudensis for therapeutic purposes.
authors.affiliation: Division of Applied Life Science (BK21 Plus), Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: Abinaya
authors.initials: A
authors.lastname: Manivannan
authors.affiliation: Division of Applied Life Science (BK21 Plus), Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: Prabhakaran
authors.initials: P
authors.lastname: Soundararajan
authors.affiliation: Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: Yoo Gyeong
authors.initials: YG
authors.lastname: Park
authors.affiliation: Division of Applied Life Science (BK21 Plus), Gyeongsang National University, Jinju 660-701, Republic of Korea ; Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 660-701, Republic of Korea ; Research Institute of Life Science, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: Byoung Ryong
authors.initials: BR
authors.lastname: Jeong
mesh_terms: D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology; D001713:Biphenyl Compounds / Q000737:chemistry; D004247:DNA / Q000187:drug effects; D005419:Flavonoids / Q000032:analysis / Q000737:chemistry; D010084:Oxidation-Reduction / Q000187:drug effects; D010636:Phenols / Q000032:analysis / Q000737:chemistry; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry*; D018520:Plant Shoots / Q000737:chemistry; D032266:Scrophularia / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D005419:Flavonoids; D010636:Phenols; D010851:Picrates; D010936:Plant Extracts; D004247:DNA; C004931:1,1-diphenyl-2-picrylhydrazyl