title: [Vitamin E prevents the toxic effect of benzo(a)pryene on reproductive system in male SD rats].
abstract: OBJECTIVE
  To investigate the protective effects and the potential mechanisms of vitamin E (VE) on benzo(a)pryene (B[a]P)-induced toxicity in the reproductive system of male rats.
  
  
  METHODS
  A total of 60 male Sprague Dawley (SD) rats, weighted 70-90 g, were randomly assigned to 6 groups: a control group, a vehicle group, a B[a]P group (5 mg/kg), a VE (10 mg/kg)+ B[a]P (5 mg/kg) group, a VE (50 mg/kg) + B[a]P (5 mg/kg) group and a VE (100 mg/kg)+B[a]P (5 mg/kg) group (n=10 per group). The rats were treated with B[a]P and/or VE once a day for 30 days via intragastric administration. The sperm quality and the levels of SOD, GSH-Px, 8-OHdG and MDA were detected, respectively. The testicular tissue morphology and DNA damage were observed by HE staining and comet assay.
  
  
  RESULTS
  The sperm count, the rate of sperm deformation, the content of MDA and 8-OHdG were all significantly increased in single B[a]P-treated group in comparison to the control groups. The activities of SOD and GSH-Px were markedly decreased by B[a]P as compared with the control groups (P<0.05). The injury of testicular tissue in B[a]P-treated rats was remarkably improved after VE treatment. The levels of oxidative stress and DNA damage indicators in the B[a]P-treated group were all attenuated by VE. These protective effects of VE were in a dose-dependent manner (P<0.05).
  
  
  CONCLUSIONS
  Vitamin E can protect the male SD rats against the B[a]P-induced reproductive toxicity.
authors.affiliation: Center for Research of Medicine & Social Development, Innovation Center for Social Risk Governance in Health, School of Public Health, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: Center for Research of Medicine & Social Development, Innovation Center for Social Risk Governance in Health, School of Public Health, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Dong
authors.affiliation: Center for Research of Medicine & Social Development, Innovation Center for Social Risk Governance in Health, School of Public Health, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Yang
authors.affiliation: Laboratory of Reproductive Biology, School of Public Health, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Mengyun
authors.initials: M
authors.lastname: Wu
authors.affiliation: Center for Research of Medicine & Social Development, Innovation Center for Social Risk Governance in Health, School of Public Health, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Baijie
authors.initials: B
authors.lastname: Tu
mesh_terms: D000818:Animals; D001564:Benzo(a)pyrene / Q000633:toxicity*; D004249:DNA Damage; D008297:Male; D018384:Oxidative Stress; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013094:Spermatozoa / Q000187:drug effects*; D013737:Testis / Q000187:drug effects / Q000473:pathology; D014810:Vitamin E / Q000494:pharmacology*
chemical_list: D014810:Vitamin E; D001564:Benzo(a)pyrene