title: Magnetically-refreshable receptor platform structures for reusable nano-biosensor chips.
abstract: We developed a magnetically-refreshable receptor platform structure which can be integrated with quite versatile nano-biosensor structures to build reusable nano-biosensor chips. This structure allows one to easily remove used receptor molecules from a biosensor surface and reuse the biosensor for repeated sensing operations. Using this structure, we demonstrated reusable immunofluorescence biosensors. Significantly, since our method allows one to place receptor molecules very close to a nano-biosensor surface, it can be utilized to build reusable carbon nanotube transistor-based biosensors which require receptor molecules within a Debye length from the sensor surface. Furthermore, we also show that a single sensor chip can be utilized to detect two different target molecules simply by replacing receptor molecules using our method. Since this method does not rely on any chemical reaction to refresh sensor chips, it can be utilized for versatile biosensor structures and virtually-general receptor molecular species.
authors.affiliation: Department of Physics and Astronomy, and Institute of Applied Physics, Seoul National University, Seoul 151-747, Korea.
authors.forename: Haneul
authors.initials: H
authors.lastname: Yoo
authors.forename: Dong Jun
authors.initials: DJ
authors.lastname: Lee
authors.forename: Dong-guk
authors.initials: DG
authors.lastname: Cho
authors.forename: Juhun
authors.initials: J
authors.lastname: Park
authors.forename: Ki Wan
authors.initials: KW
authors.lastname: Nam
authors.forename: Young Tak
authors.initials: YT
authors.lastname: Cho
authors.forename: Jae Yeol
authors.initials: JY
authors.lastname: Park
authors.forename: Xing
authors.initials: X
authors.lastname: Chen
authors.forename: Seunghun
authors.initials: S
authors.lastname: Hong
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation*; D004867:Equipment Design; D005455:Fluorescent Antibody Technique / Q000295:instrumentation*; D015200:Fluoroimmunoassay / Q000295:instrumentation; D006801:Humans; D016753:Interleukin-10 / Q000032:analysis; D015847:Interleukin-4 / Q000032:analysis; D008280:Magnetics / Q000295:instrumentation; D059346:Magnets / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D014173:Transistors, Electronic
chemical_list: D055910:Antibodies, Immobilized; D037742:Nanotubes, Carbon; D016753:Interleukin-10; D015847:Interleukin-4