title: Rare occurrence of natural transovarial transmission of dengue virus and elimination of infected foci as a possible intervention method.
abstract: Transovarial transmission of dengue virus has been studied in 33 districts of Rajasthan, India. Small proportion (1.09%) of breeding containers positive for the virus and their elimination has been demonstrated as a possible intervention method of disease control. Dengue virus was isolated from individual mosquitoes employing Indirect Fluorescence Antibody Test and Reverse Transcriptase Polymerase Chain Reaction. Out of 1,30,525 containers examined only 1432(1.09%) showed transovarially transmitted virus activity. Elimination of larvae from all the 1432 virus positive containers resulted in substantial control over prospective transmission of dengue. The study highlights rarity of transovarial transmission under natural conditions and sensitizes whether elimination of vertically infected foci could be used as a new intervention method.
authors.affiliation: Laboratory of Virology & Molecular Biology, Desert Medicine Research Centre, Indian Council of Medical Research, Jodhpur, India.
authors.forename: Annette
authors.initials: A
authors.lastname: Angel
authors.affiliation: Laboratory of Virology & Molecular Biology, Desert Medicine Research Centre, Indian Council of Medical Research, Jodhpur, India.
authors.forename: Bennet
authors.initials: B
authors.lastname: Angel
authors.affiliation: Laboratory of Virology & Molecular Biology, Desert Medicine Research Centre, Indian Council of Medical Research, Jodhpur, India. Electronic address: vinodjoshidmrc@gmail.com.
authors.forename: Vinod
authors.initials: V
authors.lastname: Joshi
mesh_terms: D000330:Aedes / Q000821:virology*; D000818:Animals; D003715:Dengue / Q000453:epidemiology / Q000517:prevention & control* / Q000635:transmission; D003716:Dengue Virus / Q000302:isolation & purification*; D006801:Humans; D007194:India / Q000453:epidemiology; D007303:Insect Vectors / Q000821:virology*; D007814:Larva / Q000821:virology; D011446:Prospective Studies
keywords: Dengue control; Elimination; New intervention module; Rarity; Vertically infected Aedes larvae