title: A quantitative determination of fluorochloridone in rat plasma by UPLC-MS/MS method: application to a pharmacokinetic study.
abstract: A precise, high-throughput and sensitive ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method has been developed for the determination of fluorochloridone (FLC) in rat plasma. The extraction of analytes from plasma samples was carried out by protein precipitation procedure using acetonitrile prior to UPLC-MS/MS analysis. Verapamil was proved as a proper internal standard (IS) among many candidates. The chromatographic separation based on UPLC was well optimized. Multiple reaction monitoring in positive electrospray ionization was used with the optimized MS transitions at: m/z 312.0 → 292.0 for FLC and m/z 456.4 → 165.2 for IS. This method was well validated with good linear response (r(2)  > 0.998) observed over the investigated range of 3-3000 ng/mL and with satisfactory stability. This method was also characterized with adequate intra- and inter-day precision and accuracy (within 12%) in the quality control samples, and with high selectivity and less matrix effect observed. Total running time was only 1.5 min. This method has been successfully applied to a pilot FLC pharmacokinetic study after oral administration. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: School of Public Health/MOE Key Lab for Public Health, Fudan University, Shanghai, 200032, People's Republic of China.
authors.forename: Shihong
authors.initials: S
authors.lastname: Liu
authors.affiliation: Pharmacology and Toxicology Department, Shanghai Institute for Food and Drug Control, Shanghai, 201203, People's Republic of China.
authors.forename: Jingmin
authors.initials: J
authors.lastname: Shi
authors.affiliation: Pharmacology and Toxicology Department, Shanghai Institute for Food and Drug Control, Shanghai, 201203, People's Republic of China.
authors.forename: Junpei
authors.initials: J
authors.lastname: Fan
authors.affiliation: Pharmacology and Toxicology Department, Shanghai Institute for Food and Drug Control, Shanghai, 201203, People's Republic of China.
authors.forename: Jie
authors.initials: J
authors.lastname: Sun
authors.affiliation: Pharmacology and Toxicology Department, Shanghai Institute for Food and Drug Control, Shanghai, 201203, People's Republic of China.
authors.forename: Suhui
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Pharmacology and Toxicology Department, Shanghai Institute for Food and Drug Control, Shanghai, 201203, People's Republic of China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Hu
authors.affiliation: School of Public Health/MOE Key Lab for Public Health, Fudan University, Shanghai, 200032, People's Republic of China.|Pharmacology and Toxicology Department, Shanghai Institute for Food and Drug Control, Shanghai, 201203, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Wei
authors.affiliation: School of Public Health/MOE Key Lab for Public Health, Fudan University, Shanghai, 200032, People's Republic of China.
authors.forename: Chunhua
authors.initials: C
authors.lastname: Wu
authors.affiliation: School of Public Health/MOE Key Lab for Public Health, Fudan University, Shanghai, 200032, People's Republic of China.
authors.forename: Xiuli
authors.initials: X
authors.lastname: Chang
authors.affiliation: Pharmacology and Toxicology Department, Shanghai Institute for Food and Drug Control, Shanghai, 201203, People's Republic of China.
authors.forename: Liming
authors.initials: L
authors.lastname: Tang
authors.affiliation: School of Public Health/MOE Key Lab for Public Health, Fudan University, Shanghai, 200032, People's Republic of China.
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Zhou
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D057230:Limit of Detection; D008297:Male; D011760:Pyrrolidinones / Q000097:blood* / Q000493:pharmacokinetics; D051381:Rats; D017208:Rats, Wistar; D012015:Reference Standards; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D011760:Pyrrolidinones; C077976:raiser
keywords: UPLC-MS/MS; fluorochloridone; pharmacokinetics; rat plasma