title: Japanese encephalitis virus induces apoptosis by the IRE1/JNK pathway of ER stress response in BHK-21 cells.
abstract: The role of endoplasmic reticulum (ER) stress in Japanese encephalitis is largely unknown. In this study, we found that Japanese encephalitis virus (JEV) strain SA14-14-2 regulates the expression of glucose-regulated protein 78 (GRP78), transcription factor 4 (ATF4) and C/EBP homologous protein (CHOP), and splicing of X-box-binding protein 1 (XBP1) mRNA in BHK-21 cells. SA14-14-2-induced cytopathic effect and decrease in viability were also observed. Moreover, the inositol-requiring enzyme 1 (IRE1) inhibitor 3,5-dibromosalicylaldehyde and JNK inhibitor SP600125 increased cell viability and reduced cell apoptosis but did not alter virus replication in SA14-14-2-infected BHK-21 cells. These results, for the first time, demonstrate that JEV induces apoptosis by the IRE1/JNK pathway of ER stress response.
authors.affiliation: College of Bioscience and Engineering, Jiangxi Agricultural University, Nanchang, Jiangxi, China.
authors.forename: Mingjie
authors.initials: M
authors.lastname: Huang
authors.affiliation: College of Bioscience and Engineering, Jiangxi Agricultural University, Nanchang, Jiangxi, China.
authors.forename: Ahui
authors.initials: A
authors.lastname: Xu
authors.affiliation: College of Bioscience and Engineering, Jiangxi Agricultural University, Nanchang, Jiangxi, China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Wu
authors.affiliation: Jiangxi Province Center for Disease Control and Prevention, Nanchang, Jiangxi, China.
authors.forename: Yanni
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Bioscience and Engineering, Jiangxi Agricultural University, Nanchang, Jiangxi, China.
authors.forename: Yunli
authors.initials: Y
authors.lastname: Guo
authors.affiliation: College of Bioscience and Engineering, Jiangxi Agricultural University, Nanchang, Jiangxi, China.
authors.forename: Fenglin
authors.initials: F
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, 430072, Hubei, China.
authors.forename: Zishu
authors.initials: Z
authors.lastname: Pan
authors.affiliation: College of Bioscience and Engineering, Jiangxi Agricultural University, Nanchang, Jiangxi, China. lingbaok@hotmail.com.
authors.forename: Lingbao
authors.initials: L
authors.lastname: Kong
mesh_terms: D000818:Animals; D017209:Apoptosis; D002460:Cell Line; D002470:Cell Survival; D006224:Cricetinae; D003588:Cytopathogenic Effect, Viral; D004664:Encephalitis Virus, Japanese / Q000254:growth & development*; D004721:Endoplasmic Reticulum / Q000502:physiology; D005347:Fibroblasts / Q000502:physiology / Q000821:virology*; D054884:Host-Pathogen Interactions; D048670:MAP Kinase Kinase 4 / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D015398:Signal Transduction; D013312:Stress, Physiological
chemical_list: D017346:Protein Serine-Threonine Kinases; D048670:MAP Kinase Kinase 4
keywords: Apoptosis; ER stress; IRE1; JEV; JNK