title: Molecular Characterization of β-Lactamase-Producing Escherichia coli Collected from 2001 to 2011 from Pigs in Korea.
abstract: To survey the diversity of plasmid-mediated β-lactamases, and the replicon types of the plasmids in Escherichia coli in the community, 95 multidrug-resistant E. coli isolates from stool samples of pigs in Korea from 2001 to 2011 were analyzed, and all isolates were confirmed as β-lactamase-producing bacteria, which mostly carried genes encoding TEM (100%), ACC (95%), SHV (62%), OXA (28%), CTX-M (26%), and NDM-1 (15%). Moreover, each isolate contained at least three β-lactamases genes on its plasmid. The polymerase chain reaction-based replicon typing method revealed that among the 18 replicon types, 15 types were identified in this work, and IncF groups (FIB; 100%, Frep; 99%) and HI1 (98%) type were predominant. Other replicon types such as Y, B/O, A/C, P, FIA, N, HI2, I1, and W were also detected; moreover at least three types were identified in each organism. In conclusion, a high prevalence of various types of β-lactamases genes were identified in E. coli from pigs in Korea, and these genes appeared to be mainly associated with IncF group, IncHI1, and IncY plasmids.
authors.affiliation: College of Veterinary Medicine, Chonnam National University , Gwangju, Korea.
authors.forename: Ji Won
authors.initials: JW
authors.lastname: Han
authors.affiliation: College of Veterinary Medicine, Chonnam National University , Gwangju, Korea.
authors.forename: Hong Bum
authors.initials: HB
authors.lastname: Koh
authors.affiliation: College of Veterinary Medicine, Chonnam National University , Gwangju, Korea.
authors.forename: Tae Jung
authors.initials: TJ
authors.lastname: Kim
mesh_terms: D000818:Animals; D004269:DNA, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D005243:Feces / Q000382:microbiology*; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D012093:Replicon; D056910:Republic of Korea; D013552:Swine / Q000382:microbiology*; D001618:beta-Lactamases / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; D001618:beta-Lactamases