title: [Proteomic analysis of curdlan-producing Agrobacterium sp. ATCC 31749 in response to dissolved oxygen].
abstract: OBJECTIVE
  Curdlan is produced by Agrobacterium sp. ATCC 31749 under nitrogen limiting condition. The biosynthesis of crudlan is a typical aerobic bioprocess, and the production of curdlan would be severely restricted under micro-aerobic and anoxic conditions. Proteomic analysis of Agrobacterium sp. was conducted to investigate the effect of dissolved oxygen on the crucial enzymes involved in curdlan biosynthesis.
  
  
  METHODS
  Two-dimensional gel electrophoresis was performed to separate and visualize the differential expression of the intracellular proteins extracted from Agrobacterium sp. ATCC 31749 cultured under various dissolved oxygen levels (75%, 50%, 25% and 5%). In addition, a comparative proteomic analysis of the intracellular proteins expression level under various dissolved oxygen levels was done. Significant differently expressed proteins were identified by MALDI-TOF/TOF.
  
  
  RESULTS
  Finally, we identified 15 differently expressed proteins involved in polysaccharide synthesis, fatty acid synthesis, amino acid synthesis pathway. Among these proteins, phosphoglucomutase and orotidine 5-phosphate decarboxylase were the key metabolic enzymes directing curdlan biosynthesis.
  
  
  CONCLUSIONS
  Oxygen could affect the expression of the proteins taking charge of curdlan synthesis significantly.
authors.forename: Xiaomeng
authors.initials: X
authors.lastname: Dai
authors.forename: Libo
authors.initials: L
authors.lastname: Yang
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Zheng
authors.forename: Haiqin
authors.initials: H
authors.lastname: Chen
authors.forename: Xiaobei
authors.initials: X
authors.lastname: Zhan
mesh_terms: D060054:Agrobacterium / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D008969:Molecular Sequence Data; D010100:Oxygen / Q000378:metabolism*; D040901:Proteomics; D047071:beta-Glucans / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D047071:beta-Glucans; C038459:curdlan; D010100:Oxygen