title: Genetic variability among Hymenolepis nana isolates from different geographical regions in China revealed by sequence analysis of three mitochondrial genes.
abstract: Hymenolepis nana is a common tapeworm that parasitizes in the small intestine of rodent animals and humans. The present study examined the sequence diversity of three mitochondrial (mt) genes namely NADH dehydrogenase subunits 5 (nad5), small subunit ribosomal RNA (rrnS), and ATPase subunit 6 (atp6) of H. nana from mice in different geographical regions of China. A part of the nad5 (pnad5), complete rrnS and atp6 genes were amplified separately from individual H. nana isolates using polymerase chain reaction (PCR) and then sequenced. The sequences of pnad5, rrnS, and atp6 were 710 bp, 704-711 bp, and 516 bp in length, respectively. The A + T contents of the sequences were 70.1-73.5% (pnad5), 70.1-71.7% (rrnS), and 76.6-77.9% (atp6). Sequence variation within H. nana was 0-1.4% for atp6, 0-1.7% for rrnS, and 0-0.7% for pnad5. The inter-specific sequence differences between H. nana and Hymenolepis diminuta were significantly higher, which was 31.6-31.7% (pnad5), 16.1-17.6% (rrnS), and 26.5-27.1% (atp6). Phylogenetic analysis based on the combined three sequences using the maximum parsimony (MP) method supported that H. nana is a species complex or "cryptic" species. These findings demonstrated clearly the usefulness of the three mtDNA sequences for population genetics and systematic studies of H. nana of human and animal health significance.
authors.affiliation: a College of Veterinary Medicine, South China Agricultural University , Guangzhou, Guangdong Province , PR China.|b State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences , Lanzhou, Gansu Province , PR China.|c Key Laboratory of Zoonosis Prevention and Control of Guangdong Province , Guangzhou, Guangdong Province , PR China.
authors.forename: Tian
authors.initials: T
authors.lastname: Cheng
authors.affiliation: b State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences , Lanzhou, Gansu Province , PR China.|d College of Animal Science and Veterinary Medicine , Heilongjiang Bayi Agricultural University , Daqing, Heilongjiang Province , PR China.
authors.forename: De-Zhen
authors.initials: DZ
authors.lastname: Gao
authors.affiliation: b State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences , Lanzhou, Gansu Province , PR China.
authors.forename: Wei-Ning
authors.initials: WN
authors.lastname: Zhu
authors.affiliation: e College of Animal Science and Technology , Hebei North University , Zhang Jiakou, Hebei Province , PR China , and.
authors.forename: Su-Fang
authors.initials: SF
authors.lastname: Fang
authors.affiliation: f Shenzhen Institute for Drug Control , Shenzhen, Guangdong Province , PR China.
authors.forename: Ning
authors.initials: N
authors.lastname: Chen
authors.affiliation: b State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences , Lanzhou, Gansu Province , PR China.
authors.forename: Xing-Quan
authors.initials: XQ
authors.lastname: Zhu
authors.affiliation: b State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences , Lanzhou, Gansu Province , PR China.
authors.forename: Guo-Hua
authors.initials: GH
authors.lastname: Liu
authors.affiliation: a College of Veterinary Medicine, South China Agricultural University , Guangzhou, Guangdong Province , PR China.|c Key Laboratory of Zoonosis Prevention and Control of Guangdong Province , Guangzhou, Guangdong Province , PR China.
authors.forename: Rui-Qing
authors.initials: RQ
authors.lastname: Lin
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics; D000818:Animals; D001482:Base Composition; D002681:China; D004272:DNA, Mitochondrial / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D050259:Genes, Mitochondrial; D014644:Genetic Variation; D048189:Hymenolepis nana / Q000145:classification / Q000235:genetics*; D051379:Mice; D009245:NADH Dehydrogenase / Q000235:genetics; D010802:Phylogeny; D058974:Phylogeography; D012335:RNA, Ribosomal / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004272:DNA, Mitochondrial; D012335:RNA, Ribosomal; D009245:NADH Dehydrogenase; D000251:Adenosine Triphosphatases
keywords: Hymenolepis nana; mitochondrial DNA (mtDNA); phylogenetic analysis; sequence variation