title: Effect of olive oil phenolic compounds on the expression of blood pressure-related genes in healthy individuals.
abstract: OBJECTIVE
  To investigate whether the ingestion of olive oil having different phenolic contents influences the expression of blood pressure-related genes, involved in the renin-angiotensin-aldosterone system, in healthy humans.
  
  
  METHODS
  A randomized, double-blind, crossover human trial with 18 healthy subjects, who ingested 25 mL/day of olive oils (1) high (366 mg/kg, HPC) and (2) low (2.7 mg/kg, LPC) in phenolic compounds for 3 weeks, preceded by 2-week washout periods. Determination of selected blood pressure-related gene expression in peripheral blood mononuclear cells (PBMNC) by qPCR, blood pressure and systemic biomarkers.
  
  
  RESULTS
  HPC decreased systolic blood pressure compared to pre-intervention values and to LPC, and maintained diastolic blood pressure values compared to LPC. HPC decreased ACE and NR1H2 gene expressions compared with pre-intervention values, and IL8RA gene expression compared with LPC.
  
  
  CONCLUSIONS
  The introduction to the diet of an extra-virgin olive oil rich in phenolic compounds modulates the expression of some of the genes related to the renin-angiotensin-aldosterone system. These changes could underlie the decrease in systolic blood pressure observed.
authors.affiliation: Cardiovascular Risk and Nutrition Research Group, REGICOR Study Group, Hospital del Mar Research Institute (IMIM), Doctor Aiguader 88, 08003, Barcelona, Spain.|Spanish Biomedical Research Networking Centre (CIBER), Physiopathology of Obesity and Nutrition (CIBERobn), Instituto de Salud Carlos III, Madrid, Spain.
authors.forename: Sandra
authors.initials: S
authors.lastname: Martín-Peláez
authors.affiliation: Cardiovascular Risk and Nutrition Research Group, REGICOR Study Group, Hospital del Mar Research Institute (IMIM), Doctor Aiguader 88, 08003, Barcelona, Spain.|Spanish Biomedical Research Networking Centre (CIBER), Physiopathology of Obesity and Nutrition (CIBERobn), Instituto de Salud Carlos III, Madrid, Spain.
authors.forename: Olga
authors.initials: O
authors.lastname: Castañer
authors.affiliation: Cardiovascular Risk and Nutrition Research Group, REGICOR Study Group, Hospital del Mar Research Institute (IMIM), Doctor Aiguader 88, 08003, Barcelona, Spain.
authors.forename: Valentini
authors.initials: V
authors.lastname: Konstantinidou
authors.affiliation: Cardiovascular and Genetic Epidemiology Research Group, REGICOR Study Group, IMIM, Doctor Aiguader 88, 08003, Barcelona, Spain.|Spanish Biomedical Research Networking Centre (CIBER), Epidemiology and Public Health (CIBEResp), Instituto de Salud Carlos III, Madrid, Spain.
authors.forename: Isaac
authors.initials: I
authors.lastname: Subirana
authors.affiliation: Cardiovascular Risk and Nutrition Research Group, REGICOR Study Group, Hospital del Mar Research Institute (IMIM), Doctor Aiguader 88, 08003, Barcelona, Spain.|Spanish Biomedical Research Networking Centre (CIBER), Physiopathology of Obesity and Nutrition (CIBERobn), Instituto de Salud Carlos III, Madrid, Spain.
authors.forename: Daniel
authors.initials: D
authors.lastname: Muñoz-Aguayo
authors.affiliation: Cardiovascular Risk and Nutrition Research Group, REGICOR Study Group, Hospital del Mar Research Institute (IMIM), Doctor Aiguader 88, 08003, Barcelona, Spain.|Spanish Biomedical Research Networking Centre (CIBER), Physiopathology of Obesity and Nutrition (CIBERobn), Instituto de Salud Carlos III, Madrid, Spain.
authors.forename: Gemma
authors.initials: G
authors.lastname: Blanchart
authors.affiliation: Cardiovascular and Genetic Epidemiology Research Group, REGICOR Study Group, IMIM, Doctor Aiguader 88, 08003, Barcelona, Spain.
authors.forename: Sonia
authors.initials: S
authors.lastname: Gaixas
authors.affiliation: Spanish Biomedical Research Networking Centre (CIBER), Physiopathology of Obesity and Nutrition (CIBERobn), Instituto de Salud Carlos III, Madrid, Spain.|Human Pharmacology and Clinical Neurosciences Research Group, IMIM, Doctor Aiguader 88, 08003, Barcelona, Spain.|Universitat Pompeu Fabra (CEXS-UPF), Barcelona, Spain.
authors.forename: Rafael
authors.initials: R
authors.lastname: de la Torre
authors.affiliation: Human Pharmacology and Clinical Neurosciences Research Group, IMIM, Doctor Aiguader 88, 08003, Barcelona, Spain.|Autonomous University of Barcelona, Barcelona, Spain.
authors.forename: Magí
authors.initials: M
authors.lastname: Farré
authors.affiliation: Spanish Biomedical Research Networking Centre (CIBER), Physiopathology of Obesity and Nutrition (CIBERobn), Instituto de Salud Carlos III, Madrid, Spain.|Department of Biochemistry and Molecular Biology, Faculty of Medicine and Odontology, University of Valencia, Avinguda Blasco Ibañez 15, 46010, Valencia, Spain.
authors.forename: Guillermo T
authors.initials: GT
authors.lastname: Sáez
authors.affiliation: Institute of Public Health and Clinical Nutrition, University of Eastern Finland, Kuopio Campus, P.O.Box 1627, 70211, Joensuu, Finland.
authors.forename: Kristina
authors.initials: K
authors.lastname: Nyyssönen
authors.affiliation: German Institute of Human Nutrition (DIFE), Potsdam-Rehbruecke, Arthur-Scheunert-Allee 114-116, 14558, Nuthetal, Germany.
authors.forename: Hans Joachim
authors.initials: HJ
authors.lastname: Zunft
authors.affiliation: Cardiovascular Risk and Nutrition Research Group, REGICOR Study Group, Hospital del Mar Research Institute (IMIM), Doctor Aiguader 88, 08003, Barcelona, Spain.|Spanish Biomedical Research Networking Centre (CIBER), Physiopathology of Obesity and Nutrition (CIBERobn), Instituto de Salud Carlos III, Madrid, Spain.
authors.forename: Maria Isabel
authors.initials: MI
authors.lastname: Covas
authors.affiliation: Cardiovascular Risk and Nutrition Research Group, REGICOR Study Group, Hospital del Mar Research Institute (IMIM), Doctor Aiguader 88, 08003, Barcelona, Spain. mfito@imim.es.|Spanish Biomedical Research Networking Centre (CIBER), Physiopathology of Obesity and Nutrition (CIBERobn), Instituto de Salud Carlos III, Madrid, Spain. mfito@imim.es.
authors.forename: Montse
authors.initials: M
authors.lastname: Fitó
mesh_terms: D000328:Adult; D000450:Aldosterone / Q000235:genetics; D015415:Biomarkers / Q000032:analysis; D001794:Blood Pressure / Q000235:genetics*; D018592:Cross-Over Studies; D004042:Dietary Fats, Unsaturated / Q000008:administration & dosage; D004311:Double-Blind Method; D015870:Gene Expression / Q000187:drug effects*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D000071518:Liver X Receptors / Q000235:genetics; D008297:Male; D008875:Middle Aged; D000069463:Olive Oil / Q000737:chemistry*; D007703:Peptidyl-Dipeptidase A / Q000235:genetics; D010636:Phenols / Q000008:administration & dosage*; D023062:Receptors, Interleukin-8A / Q000235:genetics; D012084:Renin-Angiotensin System / Q000235:genetics
chemical_list: D015415:Biomarkers; D004042:Dietary Fats, Unsaturated; D000071518:Liver X Receptors; C000605210:NR1H2 protein, human; D000069463:Olive Oil; D010636:Phenols; D023062:Receptors, Interleukin-8A; D000450:Aldosterone; C488034:ACE protein, human; D007703:Peptidyl-Dipeptidase A
keywords: Blood pressure; Nutrigenomics; Olive oil; Phenolic compounds; RAAS