title: New sesquiterpene and thiophene derivatives from Artemisia rupestris.
abstract: A new sesquiterpene, rupestrisin A (1), and three new thiophene derivatives, rupestrienes A-C (2-4), were isolated from Artemisia rupestris. Their structures were determined by analyses of MS and NMR spectroscopic data, and the absolute configuration of 1 was established by calculated ECD spectra using time-dependent density functional theory. In in vitro bioassays, compounds 1-4 showed inhibitory effects on LPS-stimulated NO production in BV-2 microglial cells with IC50 values of 24.3, 20.3, 8.5, and 5.3 μM, respectively.
authors.affiliation: a State Key Laboratory of Natural and Biomimetic Drugs , School of Pharmaceutical Sciences, Peking University , Beijing 100191 , China.
authors.forename: Chen
authors.initials: C
authors.lastname: Zhang
authors.affiliation: a State Key Laboratory of Natural and Biomimetic Drugs , School of Pharmaceutical Sciences, Peking University , Beijing 100191 , China.
authors.forename: Bing-Yu
authors.initials: BY
authors.lastname: Liu
authors.affiliation: a State Key Laboratory of Natural and Biomimetic Drugs , School of Pharmaceutical Sciences, Peking University , Beijing 100191 , China.
authors.forename: Ke-Wu
authors.initials: KW
authors.lastname: Zeng
authors.affiliation: a State Key Laboratory of Natural and Biomimetic Drugs , School of Pharmaceutical Sciences, Peking University , Beijing 100191 , China.
authors.forename: Xiao-Yu
authors.initials: XY
authors.lastname: Guo
authors.affiliation: a State Key Laboratory of Natural and Biomimetic Drugs , School of Pharmaceutical Sciences, Peking University , Beijing 100191 , China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: a State Key Laboratory of Natural and Biomimetic Drugs , School of Pharmaceutical Sciences, Peking University , Beijing 100191 , China.
authors.forename: Peng-Fei
authors.initials: PF
authors.lastname: Tu
mesh_terms: D000818:Animals; D019306:Artemisia / Q000737:chemistry; D020128:Inhibitory Concentration 50; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D017628:Microglia / Q000187:drug effects; D015394:Molecular Structure; D009569:Nitric Oxide / Q000096:biosynthesis; D019906:Nuclear Magnetic Resonance, Biomolecular; D012717:Sesquiterpenes / Q000737:chemistry / Q000302:isolation & purification*; D013876:Thiophenes / Q000494:pharmacology
chemical_list: D008070:Lipopolysaccharides; D012717:Sesquiterpenes; D013876:Thiophenes; C000607634:rupestriene A; C000607635:rupestriene B; C000607636:rupestriene C; C000607633:rupestrisin A; D009569:Nitric Oxide
keywords: Artemisia rupestris; Compositae; NO inhibition; sesquiterpene; thiophene derivatives