title: Measurement of the Nucleus Area and Nucleus/Cytoplasm and Mitochondria/Nucleus Ratios in Human Colon Tissues by Dual-Colour Two-Photon Microscopy Imaging.
abstract: We developed two-photon (TP) probes for DNA (ABI-Nu), cytoplasm (Pyr-CT), and mitochondria (BF-MT). We found that ABI-Nu binds to AT in the minor groove, while ABI-Nu and BF-MT are effective for tracking in the cytoplasm and mitochondria, respectively. These probes showed very large effective two-photon action cross section values of 2230, 1555, and 790 Göppert-Mayer units (1 GM  =  10(-50) cm(4) s photon(-1) molecule(-1)) at 740 nm with emission maxima at 473, 561, and 560 nm, respectively, in each organelle. Using these probes, we quantitatively estimated the mean nuclear area and the ratios of nuclei to cytoplasm and mitochondria to nuclei in human colon tissues by dual-colour two-photon microscopy imaging within 2  h after biopsy. The mean nuclear area and the nuclei to cytoplasm and mitochondria to cytoplasm ratios increased in the following order: normal colon mucosa <colon adenoma <colon adenocarcinoma. Furthermore, the nuclear areas of these tissues showed significant differences that were well outside of the ranges of experimental errors, indicating the diagnostic potential of this method.
authors.affiliation: Department of Chemistry, Korea University, 145 Anam-ro, Sungbuk-gu, Seoul 136-713, Korea.
authors.forename: Chang
authors.initials: C
authors.lastname: Su Lim
authors.affiliation: Department of Internal Medicine, Korea University College of Medicine, 73 Inchon-ro, Seoul, 136-705, Korea.
authors.forename: Eun
authors.initials: E
authors.lastname: Sun Kim
authors.affiliation: Department of Chemistry, Korea University, 145 Anam-ro, Sungbuk-gu, Seoul 136-713, Korea.
authors.forename: Ji
authors.initials: J
authors.lastname: Yeon Kim
authors.affiliation: KU-KIST Graduate School of Converging Science and Technology, Korea University, 145 Anam-ro, Sungbuk-gu, Seoul 136-713, Korea.
authors.forename: Seung
authors.initials: S
authors.lastname: Taek Hong
authors.affiliation: Department of Internal Medicine, Korea University College of Medicine, 73 Inchon-ro, Seoul, 136-705, Korea.
authors.forename: Hoon
authors.initials: H
authors.lastname: Jai Chun
authors.affiliation: Department of Chemistry, Korea University, 145 Anam-ro, Sungbuk-gu, Seoul 136-713, Korea.
authors.forename: Dong
authors.initials: D
authors.lastname: Eun Kang
authors.affiliation: Department of Chemistry, Korea University, 145 Anam-ro, Sungbuk-gu, Seoul 136-713, Korea.
authors.forename: Bong
authors.initials: B
authors.lastname: Rae Cho
mesh_terms: D002467:Cell Nucleus / Q000378:metabolism*; D003106:Colon / Q000378:metabolism*; D003110:Colonic Neoplasms / Q000175:diagnosis / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism*; D004247:DNA / Q000378:metabolism; D003952:Diagnostic Imaging / Q000379:methods; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D008928:Mitochondria / Q000378:metabolism*; D015394:Molecular Structure; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D005456:Fluorescent Dyes; D004247:DNA