title: Endogenous conversion of ω-6 to ω-3 polyunsaturated fatty acids in fat-1 mice attenuated intestinal polyposis by either inhibiting COX-2/β-catenin signaling or activating 15-PGDH/IL-18.
abstract: Omega-3 polyunsaturated fatty acids (ω-3PUFAs) have inhibitory effects in various preclinical cancer models, but their effects in intestinal polyposis have never been examined. As attempts have been made to use nutritional intervention to counteract colon cancer development, in this study we evaluated the effects of ω-3 PUFAs on intestinal polyposis in the Apc(Min/+) mouse model. The experimental groups included wild-type C56BL/6 mice, Apc(Min/+) mice, fat-1 transgenic mice expressing an n-3 desaturase to enable ω-3 PUFA synthesis, and Apc(Min/+) × fat-1 double-transgenic mice; all mice were 20 weeks of age. Small intestines were collected for gross and pathologic evaluation, including assessment of polyp number and size, followed by immunohistochemical staining and Western blotting. After administration of various concentrations of ω-3 PUFAs, PUFA levels were measured in small intestine tissue by GC/MS/MS analysis to compare with PUFA synthesis of between C57BL6 and fat-1mice. As a result, ω-3 PUFAs significantly attenuated Apc mutation-induced intestinal polyposis accompanied with significant inhibition of Wnt/β-catenin signaling, COX-2 and PGE2, but induced significant levels of 15-PGDH. In addition, significant induction of the inflammasome-related substrates as IL-1β and IL-18 and activation of caspase-1 was observed in Apc(Min/+) × fat-1 mice. Administration of at least 3 g/60 kg ω-3 PUFAs was equivalent to ω-3 PUFAs produced in fat-1 mice and resulted in significant increase in the expression of IL-1β, caspase-3 and IL-18, as seen in Apc(Min/+) × fat-1 mice. We conclude that ω-3PUFAs can prevent intestinal polyp formation by inhibition of Wnt/β-catenin signaling, but increased levels of 15-PGDH and IL-18.
authors.affiliation: CHA Cancer Prevention Research Center, CHA Cancer Institute, CHA University, Seoul, Korea.
authors.forename: Young-Min
authors.initials: YM
authors.lastname: Han
authors.affiliation: CHA Cancer Prevention Research Center, CHA Cancer Institute, CHA University, Seoul, Korea.
authors.forename: Jong-Min
authors.initials: JM
authors.lastname: Park
authors.affiliation: Department of Molecular Medicine, Lee Gil Ya Cancer and Diabetes Institute, Gachon University, Incheon, Korea.
authors.forename: Ji-Young
authors.initials: JY
authors.lastname: Cha
authors.affiliation: CHA Cancer Prevention Research Center, CHA Cancer Institute, CHA University, Seoul, Korea.
authors.forename: Migyeong
authors.initials: M
authors.lastname: Jeong
authors.affiliation: CHA Cancer Prevention Research Center, CHA Cancer Institute, CHA University, Seoul, Korea.
authors.forename: Eun-Jin
authors.initials: EJ
authors.lastname: Go
authors.affiliation: CHA Cancer Prevention Research Center, CHA Cancer Institute, CHA University, Seoul, Korea.|Digestive Disease Center, CHA University Bundang Medical Center, Seongnam, Korea.
authors.forename: Ki Baik
authors.initials: KB
authors.lastname: Hahm
mesh_terms: D000818:Animals; D002849:Chromatography, Gas; D051546:Cyclooxygenase 2 / Q000378:metabolism; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D015525:Fatty Acids, Omega-3 / Q000378:metabolism*; D043371:Fatty Acids, Omega-6 / Q000378:metabolism*; D006911:Hydroxyprostaglandin Dehydrogenases / Q000378:metabolism; D020287:In Situ Nick-End Labeling; D020382:Interleukin-18 / Q000378:metabolism; D044483:Intestinal Polyposis / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D015398:Signal Transduction / Q000502:physiology*; D053719:Tandem Mass Spectrometry; D051176:beta Catenin / Q000378:metabolism
chemical_list: C495267:CTNNB1 protein, mouse; D015525:Fatty Acids, Omega-3; D043371:Fatty Acids, Omega-6; D020382:Interleukin-18; D051176:beta Catenin; D006911:Hydroxyprostaglandin Dehydrogenases; C035859:15-hydroxyprostaglandin dehydrogenase; C077623:Ptgs2 protein, mouse; D051546:Cyclooxygenase 2
keywords: 15-PGDH; ApcMin/+mice; IL-18; fat-1 transgenic mice; ω-3 PUFAs