title: Role of Micro RNA-205 in Promoting Visceral Adiposity of NZ10 Mice with Polygenic Susceptibility for Type 2 Diabetes.
abstract: METHODS
  To characterize diet-dependent miRNA profiles and their targets in the visceral adipose of mice with polygenic susceptibility to type 2 diabetes.
  
  
  RESULTS
  Six-week NONcNZO10/LtJ (NZ10) and control SWR/J mice were subjected to high protein-fish oil or control diets for 19 weeks and micro-RNA microarray analyses were implemented on visceral adipose RNA. We found that 27 miRNAs were significantly induced and 10 significantly repressed in the VA of obese NZ10 mice compared with controls. 12 selected regulated miRNAs were confirmed by RT-PCR based on the microarray data and we demonstrated that the expression of these miRNAs remained unaltered in the VA of control SWR mice. To assess the possible functional roles of miRNAs in adipogenesis, we also analyzed their expression in 3T3-L1 cells during growth and differentiation. This revealed that suppression of miRNA-205 alone correlated selectively with increased cell proliferation and lipid formation of adipocytes.
  
  
  CONCLUSIONS
  Diet and genetics control the expression of obesity-regulated miRNAs in the visceral adipose of NZ10 mice.
authors.affiliation: Department of Molecular and Cellular Pharmacology, Miller School of Medicine, University of Miami, Miami, FL, USA ; Vascular Biology Institute, Miller School of Medicine, University of Miami, Miami, FL, USA.
authors.forename: Nikhil
authors.initials: N
authors.lastname: Adi
authors.affiliation: Department of Molecular and Cellular Pharmacology, Miller School of Medicine, University of Miami, Miami, FL, USA ; Vascular Biology Institute, Miller School of Medicine, University of Miami, Miami, FL, USA.
authors.forename: Jennipher
authors.initials: J
authors.lastname: Adi
authors.affiliation: Vascular Biology Institute, Miller School of Medicine, University of Miami, Miami, FL, USA.
authors.forename: Liliana
authors.initials: L
authors.lastname: Cesar
authors.affiliation: Columbia University, New York, USA.
authors.forename: Paul
authors.initials: P
authors.lastname: Kurlansky
authors.affiliation: Miami Baptist Hospital, Miami, FL, USA.
authors.forename: Arthur
authors.initials: A
authors.lastname: Agatston
authors.affiliation: Department of Molecular and Cellular Pharmacology, Miller School of Medicine, University of Miami, Miami, FL, USA ; Vascular Biology Institute, Miller School of Medicine, University of Miami, Miami, FL, USA.
authors.forename: Keith A
authors.initials: KA
authors.lastname: Webster
keywords: 3t3-l1; Adipogenesis; Micro-RNA; Obesity; Visceral adipose