title: Three dimensional biphasic calcium phosphate nanocomposites for load bearing bioactive bone grafts.
abstract: Mimicking matrix mediated bio-mineralization process, three dimensional blocks of biphasic calcium phosphate (BCP, hydroxyapatite (HA) and β-tricalcium phosphate (TCP)) nanocomposites, having three different stoichiometries have been synthesized for possible application as load bearing synthetic bone graft or scaffolds. Biphasic blocks with three weight ratios of 20:80, 25:75 and 30:70 of HA and TCP respectively have been synthesized. Detailed structural and chemical characterization of the samples revealed a strong dependence of porosity and mechanical properties on the stoichiometry of biphasic blocks. Effect of physiological medium on the microstructure and mechanical properties of the three different blocks has also been studied. Bioactivity of the BCP block, exhibiting highest compressive strength in air as well as in physiological medium, has been evaluated through adhesion, proliferation and differentiation of mesenchymal stem cells using different markers.
authors.affiliation: Materials Science and Technology Division, CSIR-National Metallurgical Laboratory, Jamshedpur 831007, India. Electronic address: subha@nmlindia.org.
authors.forename: Subhadra
authors.initials: S
authors.lastname: Garai
authors.affiliation: Materials Science and Technology Division, CSIR-National Metallurgical Laboratory, Jamshedpur 831007, India.
authors.forename: Arvind
authors.initials: A
authors.lastname: Sinha
mesh_terms: D000818:Animals; D018786:Bone Substitutes / Q000737:chemistry*; D006882:Hydroxyapatites / Q000737:chemistry*; D008297:Male; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D053761:Nanocomposites / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D016474:Weight-Bearing
chemical_list: D018786:Bone Substitutes; D006882:Hydroxyapatites; C074950:hydroxyapatite-beta tricalcium phosphate
keywords: Biphasic calcium phosphate; Calcium-deficient hydroxyapatite; Cellulose polymer; Porous scaffolds