title: Colistin-Resistant Acinetobacter baumannii Clinical Strains with Deficient Biofilm Formation.
abstract: In two pairs of clinical colistin-susceptible/colistin-resistant (Cst(s)/Cst(r)) Acinetobacter baumannii strains, the Cst(r) strains showed significantly decreased biofilm formation in static and dynamic assays (P < 0.001) and lower relative fitness (P < 0.05) compared with those of the Cst(s) counterparts. The whole-genome sequencing comparison of strain pairs identified a mutation converting a stop codon to lysine (*241K) in LpsB (involved in lipopolysaccharide [LPS] synthesis) in one Cst(r) strain and a frameshift mutation in CarO and the loss of a 47,969-bp element containing multiple genes associated with biofilm production in the other.
authors.affiliation: Department of Microbiology, National and Kapodistrian University of Athens, Athens, Greece Laboratory of Medical Microbiology, Vaccine and Infectious Disease Institute, University of Antwerp, Antwerp, Belgium.
authors.forename: Konstantina
authors.initials: K
authors.lastname: Dafopoulou
authors.affiliation: Laboratory of Medical Microbiology, Vaccine and Infectious Disease Institute, University of Antwerp, Antwerp, Belgium.
authors.forename: Basil Britto
authors.initials: BB
authors.lastname: Xavier
authors.affiliation: Laboratory of Medical Microbiology, Vaccine and Infectious Disease Institute, University of Antwerp, Antwerp, Belgium.
authors.forename: An
authors.initials: A
authors.lastname: Hotterbeekx
authors.affiliation: Laboratory of Medical Microbiology, Vaccine and Infectious Disease Institute, University of Antwerp, Antwerp, Belgium.
authors.forename: Lore
authors.initials: L
authors.lastname: Janssens
authors.affiliation: Laboratory of Medical Microbiology, Vaccine and Infectious Disease Institute, University of Antwerp, Antwerp, Belgium.
authors.forename: Christine
authors.initials: C
authors.lastname: Lammens
authors.affiliation: CNRS UMR 6270, Polymers, Biopolymers and Surfaces Laboratory, University of Rouen, Rouen, France.
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Dé
authors.affiliation: Laboratory of Medical Microbiology, Vaccine and Infectious Disease Institute, University of Antwerp, Antwerp, Belgium.
authors.forename: Herman
authors.initials: H
authors.lastname: Goossens
authors.affiliation: Department of Microbiology, National and Kapodistrian University of Athens, Athens, Greece.
authors.forename: Athanasios
authors.initials: A
authors.lastname: Tsakris
authors.affiliation: Laboratory of Medical Microbiology, Vaccine and Infectious Disease Institute, University of Antwerp, Antwerp, Belgium.
authors.forename: Surbhi
authors.initials: S
authors.lastname: Malhotra-Kumar
authors.affiliation: Department of Microbiology, National and Kapodistrian University of Athens, Athens, Greece spournaras@med.uoa.gr.
authors.forename: Spyros
authors.initials: S
authors.lastname: Pournaras
mesh_terms: D000151:Acinetobacter Infections / Q000188:drug therapy / Q000382:microbiology; D040981:Acinetobacter baumannii / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001422:Bacterial Adhesion / Q000235:genetics; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D018441:Biofilms / Q000187:drug effects / Q000254:growth & development*; D003091:Colistin / Q000494:pharmacology*; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D006801:Humans; D008070:Lipopolysaccharides / Q000096:biosynthesis; D008364:Mannosyltransferases / Q000235:genetics; D008826:Microbial Sensitivity Tests; D018272:Porins / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; C561703:CarO protein, Acinetobacter baumannii; D008070:Lipopolysaccharides; D018272:Porins; C422469:LpsB protein, bacteria; D008364:Mannosyltransferases; D003091:Colistin