title: Isoorientin, a Selective Inhibitor of Cyclooxygenase-2 (COX-2) from the Tubers of Pueraria tuberosa.
abstract: Bioassay-guided fraction of the methanol extract of the roots of Pueraria tuberose DC yielded puerarin, an isoflavone C-glycoside (PT-1), isoorientin, a flavone C-glycoside (PT-2) and mangiferin, a xanthone C-glycoside (PT-3). The extracts and the isolated compounds were screened for potent anti-inflammatory components inhibiting the cyclooxygenases (COX-1 and COX-2) and 5-lipoxygenase (5-LOX), the target enzymes of inflammation, by employing spectroscopic/polorographic methods. Among these, isoorientin was found to be a potent inhibitor of COX-2with an IC50 value of 39 μM. Docking studies were carried out to understand the interactions of isorientin (PT-2) with COX-2.The structures of the isolates were determined by mass spectrometry and 2D-NMR techniques including HSQC, HMBC, NOESY and 1H-1H COSY experiments. Although isoorientin and mangiferin have been reported from several plant sources, this is the first report of their isolation from a Pueraria species.
authors.forename: Manne
authors.initials: M
authors.lastname: Sumalatha
authors.forename: Rachakunta
authors.initials: R
authors.lastname: Munikishore
authors.forename: Aluru
authors.initials: A
authors.lastname: Rammohan
authors.forename: Duvvuru
authors.initials: D
authors.lastname: Gunasekar
authors.forename: Kotha Anil
authors.initials: KA
authors.lastname: Kumar
authors.forename: Kakularam Kumar
authors.initials: KK
authors.lastname: Reddy
authors.forename: Rajaram
authors.initials: R
authors.lastname: Azad
authors.forename: Pallu
authors.initials: P
authors.lastname: Reddanna
authors.forename: Bernard
authors.initials: B
authors.lastname: Bodo
mesh_terms: D051546:Cyclooxygenase 2 / Q000378:metabolism; D052246:Cyclooxygenase 2 Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D047311:Luteolin / Q000737:chemistry* / Q000494:pharmacology; D008958:Models, Molecular; D015394:Molecular Structure; D035281:Plant Tubers / Q000737:chemistry*; D029908:Pueraria / Q000737:chemistry*; D011994:Recombinant Proteins
chemical_list: D052246:Cyclooxygenase 2 Inhibitors; D011994:Recombinant Proteins; C057912:homoorientin; D051546:Cyclooxygenase 2; D047311:Luteolin