title: Application of Differential Proteomic Analysis to Authenticate Ophiocordyceps sinensis.
abstract: Ophiocordyceps sinensis (Berk.) Sacc. is one of the most well-known fungi in traditional Chinese medicine and is attracting attention because of its nutritious and medicinal properties. The present study aimed to produce a proteomic map to identify common O. sinensis proteins. The caterpillar body and stroma of O. sinensis collected from five locations and four fungal specimens of similar appearance were examined by two-dimensional electrophoresis (2-DE). Five proteins were identified using MALDI-TOF--TOF/MS, and the 2-DE identification pattern was provided. OCS_04585 and β-lactamase domain-containing protein, the two abundant and characteristic proteins, were separated and purified using liquid-phase isoelectric focusing. The products were high-quality materials that can be used for future protein-function studies and immunoassay development.
authors.affiliation: Shenzhen Academy of Metrology and Quality Inspection, Shenzhen, 518102, People's Republic of China.
authors.forename: Shiwei
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Shenzhen Academy of Metrology and Quality Inspection, Shenzhen, 518102, People's Republic of China. zsw_8506@qq.com.
authors.forename: Xintian
authors.initials: X
authors.lastname: Lai
authors.affiliation: Shenzhen Academy of Metrology and Quality Inspection, Shenzhen, 518102, People's Republic of China.
authors.forename: Bifang
authors.initials: B
authors.lastname: Li
authors.affiliation: Shenzhen Academy of Metrology and Quality Inspection, Shenzhen, 518102, People's Republic of China.
authors.forename: Cong
authors.initials: C
authors.lastname: Wu
authors.affiliation: Shenzhen Academy of Metrology and Quality Inspection, Shenzhen, 518102, People's Republic of China.
authors.forename: Shifeng
authors.initials: S
authors.lastname: Wang
authors.affiliation: Shenzhen Academy of Metrology and Quality Inspection, Shenzhen, 518102, People's Republic of China.
authors.forename: Xuejian
authors.initials: X
authors.lastname: Chen
authors.affiliation: Shenzhen Academy of Metrology and Quality Inspection, Shenzhen, 518102, People's Republic of China.
authors.forename: Jingmin
authors.initials: J
authors.lastname: Huang
authors.affiliation: Shenzhen Academy of Metrology and Quality Inspection, Shenzhen, 518102, People's Republic of China.
authors.forename: Guowu
authors.initials: G
authors.lastname: Yang
mesh_terms: D015180:Electrophoresis, Gel, Two-Dimensional; D005656:Fungal Proteins / Q000302:isolation & purification; D006999:Hypocreales / Q000737:chemistry* / Q000145:classification* / Q000302:isolation & purification; D007525:Isoelectric Focusing; D008828:Microbiological Techniques / Q000379:methods*; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D005656:Fungal Proteins; D020543:Proteome