title: Identification of Islet Antigen-Specific CD8 T Cells Using MHCI-Peptide Tetramer Reagents in the Non Obese Diabetic (NOD) Mouse Model of Type 1 Diabetes.
abstract: MHCI-peptide tetramer staining is an important technique in order to identify antigen-specific T cells within a heterogeneous cell population. The reagents may be used to isolate antigen-specific T cells and can help identify their role in disease. Here, we describe how to make tetramer from peptide:MHC monomers together with a protocol for staining antigen-specific cell populations with advice on generating a complementary antibody phenotyping panel.
authors.affiliation: Institute of Molecular and Experimental Medicine, Cardiff School of Medicine, Cardiff University, Tenovus Building, Heath Park, CF14 4XN, UK.
authors.forename: James A
authors.initials: JA
authors.lastname: Pearson
authors.affiliation: Institute of Molecular and Experimental Medicine, Cardiff School of Medicine, Cardiff University, Tenovus Building, Heath Park, CF14 4XN, UK. Wongfs@cardiff.ac.uk.
authors.forename: F Susan
authors.initials: FS
authors.lastname: Wong
mesh_terms: D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D003922:Diabetes Mellitus, Type 1 / Q000276:immunology*; D004195:Disease Models, Animal; D005434:Flow Cytometry / Q000379:methods*; D015395:Histocompatibility Antigens Class I / Q000276:immunology*; D007515:Islets of Langerhans / Q000276:immunology*; D051379:Mice; D016688:Mice, Inbred NOD; D008822:Mice, Transgenic; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D015395:Histocompatibility Antigens Class I
keywords: Antigen-specific; Flow cytometry; MHCI-peptide tetramer staining; T cell receptor