title: Measurement of Glucose Uptake in Cultured Cells.
abstract: Facilitative glucose uptake transport systems are ubiquitous in animal cells and are responsible for transporting glucose across cell surface membranes. Evaluation of glucose uptake is crucial in the study of numerous diseases and metabolic disorders such as myocardial ischemia, diabetes mellitus, and cancer. Detailed in this unit are laboratory methods for assessing glucose uptake into mammalian cells. The unit is divided into five sections: (1) a brief overview of glucose uptake assays in cultured cells; (2) a method for measuring glucose uptake using radiolabeled 3-O-methylglucose; (3) a method for measuring glucose uptake using radiolabeled 2-deoxyglucose (2DG); (4) a microplate method for measuring 2DG-uptake using an enzymatic, fluorometric assay; and (5) a microplate-based method using a fluorescent analog of 2DG.
authors.affiliation: Research & Development Institute, House Wellness Foods Corporation, Hyogo, Japan.
authors.forename: Norio
authors.initials: N
authors.lastname: Yamamoto
authors.affiliation: National Food Research Institute, National Agriculture and Food Research Organization, Tsukuba, Japan.
authors.forename: Manabu
authors.initials: M
authors.lastname: Ueda-Wakagi
authors.affiliation: Research & Development Institute, House Wellness Foods Corporation, Hyogo, Japan.
authors.forename: Takuya
authors.initials: T
authors.lastname: Sato
authors.affiliation: Research & Development Institute, House Wellness Foods Corporation, Hyogo, Japan.
authors.forename: Kengo
authors.initials: K
authors.lastname: Kawasaki
authors.affiliation: Department of Agrobiosciences, Graduate School of Agricultural Science, Kobe University, Kobe, Japan.
authors.forename: Keisuke
authors.initials: K
authors.lastname: Sawada
authors.affiliation: Department of Bioscience, Fukui Prefectural University, Fukui, Japan.
authors.forename: Kyuichi
authors.initials: K
authors.lastname: Kawabata
authors.affiliation: Department of Biological Chemistry, Division of Applied Life Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai, Japan.
authors.forename: Mitsugu
authors.initials: M
authors.lastname: Akagawa
authors.affiliation: Department of Agrobiosciences, Graduate School of Agricultural Science, Kobe University, Kobe, Japan.
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Ashida
mesh_terms: D019325:3-O-Methylglucose / Q000378:metabolism; D000818:Animals; D001692:Biological Transport / Q000502:physiology*; D002478:Cells, Cultured; D003847:Deoxyglucose / Q000378:metabolism; D005456:Fluorescent Dyes / Q000378:metabolism; D005470:Fluorometry / Q000379:methods*; D005947:Glucose / Q000378:metabolism*; D006801:Humans
chemical_list: D005456:Fluorescent Dyes; D019325:3-O-Methylglucose; D003847:Deoxyglucose; D005947:Glucose
keywords: 2-NBDG,fluorescence; 2-deoxyglucose; 3-O-methylglucose; cell-based assay; glucose uptake; nonradioisotope; radioisotope