title: Generation of Induced Pluripotent Stem Cells From Patients With Duchenne Muscular Dystrophy and Their Induction to Cardiomyocytes.
abstract: Duchenne muscular dystrophy (DMD) is caused by mutations in the DMD gene which encodes dystrophin protein. Dystrophin defect affects cardiac muscle as well as skeletal muscle. Cardiac dysfunction is observed in all patients with DMD over 18 years of age, but there is no curative treatment for DMD cardiomyopathy. To establish novel experimental platforms which reproduce the cardiac phenotype of DMD patients, here we established iPS cell lines from T lymphocytes donated from two DMD patients, with a protocol using Sendai virus vectors. We successfully conducted the differentiation of the DMD patient-specific iPS cells into beating cardiomyocytes. DMD patient-specific iPS cells and iPS cell-derived cardiomyocytes would be a useful in vitro experimental system with which to investigate DMD cardiomyopathy.
authors.affiliation: Department of Cardiovascular Regenerative Medicine, Osaka University Graduate School of Medicine.
authors.forename: Akihito
authors.initials: A
authors.lastname: Hashimoto
authors.forename: Atsuhiko T
authors.initials: AT
authors.lastname: Naito
authors.forename: Jong-Kook
authors.initials: JK
authors.lastname: Lee
authors.forename: Rika
authors.initials: R
authors.lastname: Kitazume-Taneike
authors.forename: Masamichi
authors.initials: M
authors.lastname: Ito
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Yamaguchi
authors.forename: Ryo
authors.initials: R
authors.lastname: Nakata
authors.forename: Tomokazu
authors.initials: T
authors.lastname: Sumida
authors.forename: Katsuki
authors.initials: K
authors.lastname: Okada
authors.forename: Akito
authors.initials: A
authors.lastname: Nakagawa
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Higo
authors.forename: Yuki
authors.initials: Y
authors.lastname: Kuramoto
authors.forename: Taku
authors.initials: T
authors.lastname: Sakai
authors.forename: Koji
authors.initials: K
authors.lastname: Tominaga
authors.forename: Takeshi
authors.initials: T
authors.lastname: Okinaga
authors.forename: Shigetoyo
authors.initials: S
authors.lastname: Kogaki
authors.forename: Keiichi
authors.initials: K
authors.lastname: Ozono
authors.forename: Shigeru
authors.initials: S
authors.lastname: Miyagawa
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Sawa
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Sakata
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Morita
authors.forename: Akihiro
authors.initials: A
authors.lastname: Umezawa
authors.forename: Issei
authors.initials: I
authors.lastname: Komuro
mesh_terms: D000293:Adolescent; D000328:Adult; D002454:Cell Differentiation; D002478:Cells, Cultured; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology / Q000502:physiology*; D020388:Muscular Dystrophy, Duchenne / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000378:metabolism; D012313:RNA / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D012313:RNA