title: Circulating cell-free nucleic acids as biomarkers in colorectal cancer screening and diagnosis.
abstract: Screening methods for the most frequent diagnosed malignant tumor, colorectal cancer (CRC), have limitations. Circulating cell-free DNA (cfDNA) analysis came into focus as a potential screening test for CRC. Detection of epigenetic and genetic alterations of cfDNA as DNA methylation or DNA mutations and related ribonucleic acids may improve cancer detection based on unique, CRC-specific patterns. In this review the authors summarize the CRC-specific nucleic acid biomarkers measured in peripheral blood and their potential as screening markers. Detection of DNA mutation has inadequate sensitivity; however, methylated DNA can be established with higher sensitivity from CRC plasma samples. The ribonucleic acid based miRNA studies represented higher sensitivity for CRC as compared with mRNA studies. Recently, isolation of cfDNA has become automated, highly reproducible and a high throughput method. With automated possible diagnostic tools, a new approach may be available for CRC screening as liquid biopsy.
authors.affiliation: a 2nd Department of Internal Medicine , Semmelweis University , Budapest , Hungary.
authors.forename: Kinga
authors.initials: K
authors.lastname: Tóth
authors.affiliation: a 2nd Department of Internal Medicine , Semmelweis University , Budapest , Hungary.
authors.forename: Barbara Kinga
authors.initials: BK
authors.lastname: Barták
authors.affiliation: a 2nd Department of Internal Medicine , Semmelweis University , Budapest , Hungary.|b Molecular Medicine Research Unit , Hungarian Academy of Sciences , Budapest , Hungary.
authors.forename: Zsolt
authors.initials: Z
authors.lastname: Tulassay
authors.affiliation: a 2nd Department of Internal Medicine , Semmelweis University , Budapest , Hungary.|b Molecular Medicine Research Unit , Hungarian Academy of Sciences , Budapest , Hungary.
authors.forename: Béla
authors.initials: B
authors.lastname: Molnár
mesh_terms: D014408:Biomarkers, Tumor / Q000097:blood* / Q000235:genetics; D015179:Colorectal Neoplasms / Q000097:blood* / Q000235:genetics; D019175:DNA Methylation; D006801:Humans; D009154:Mutation; D009696:Nucleic Acids / Q000097:blood*; D059467:Transcriptome
chemical_list: D014408:Biomarkers, Tumor; D009696:Nucleic Acids
keywords: Colorectal cancer; DNA genetic alterations; RNA expression alterations; circulating cell-free DNA; screening