title: Role of Cytochrome P450 Hydroxylase in the Decreased Accumulation of Vitamin E in Muscle from Turkeys Compared to that from Chickens.
abstract: Turkeys and chickens reared to 5 weeks of age and fed diets with feedstuffs low in endogenous tocopherols were examined. Treatments included feed supplemented with RRR (natural source vitamin E) alpha tocopheryl acetate (AcT, 35 mg/kg feed) and all-racemic (synthetic vitamin E) AcT (10 and 58 mg/kg feed). Alpha tocopherol hydroxylase activity was greater in liver microsomes prepared from turkeys compared to that from chickens (p < 0.01). Alpha and gamma tocopherol metabolites were higher in turkey bile than in chicken when assessing the RRR AcT diet and the all-racemic AcT diet at 58 mg/kg feed (p < 0.01). Turkey cytochrome P450 2C29 was increased relative to its chicken ortholog on the basis of RNA-Seq transcript abundance (p < 0.001) and activity-based protein profiling (p < 0.01) of liver tissue. Alpha tocopherol concentrations in plasma, liver, and muscle from turkey were lower than the respective tissues from chicken (p < 0.05). Lipid oxidation was greater in turkey thigh than in chicken (p < 0.05). These results suggest that elevated tocopherol metabolism by cytochrome P450 hydroxylase(s) in turkeys contributes to the decreased accumulation of alpha tocopherol in turkey tissues compared to that of chickens.
authors.affiliation: Department of Animal Sciences, University of Wisconsin-Madison , Madison, Wisconsin 53706, United States.
authors.forename: Dale M
authors.initials: DM
authors.lastname: Perez
authors.affiliation: Department of Animal Sciences, University of Wisconsin-Madison , Madison, Wisconsin 53706, United States.
authors.forename: Mark P
authors.initials: MP
authors.lastname: Richards
authors.affiliation: Division of Nutritional Sciences, Cornell University , Ithaca, New York 14850, United States.
authors.forename: Robert S
authors.initials: RS
authors.lastname: Parker
authors.affiliation: Department of Animal Sciences, University of Wisconsin-Madison , Madison, Wisconsin 53706, United States.
authors.forename: Mark E
authors.initials: ME
authors.lastname: Berres
authors.affiliation: Biological Sciences Division, Pacific Northwest National Laboratory , Richland, Washington 99354, United States.
authors.forename: Aaron T
authors.initials: AT
authors.lastname: Wright
authors.affiliation: Animal Nutrition Division, Archer Daniels Midland Co. , Quincy, Illinois 62301, United States.
authors.forename: Mamduh
authors.initials: M
authors.lastname: Sifri
authors.affiliation: Biological Sciences Division, Pacific Northwest National Laboratory , Richland, Washington 99354, United States.
authors.forename: Natalie C
authors.initials: NC
authors.lastname: Sadler
authors.affiliation: Department of Animal Sciences, University of Wisconsin-Madison , Madison, Wisconsin 53706, United States.
authors.forename: Nantawat
authors.initials: N
authors.lastname: Tatiyaborworntham
authors.affiliation: Department of Animal Sciences, University of Wisconsin-Madison , Madison, Wisconsin 53706, United States.
authors.forename: Na
authors.initials: N
authors.lastname: Li
mesh_terms: D000818:Animals; D002645:Chickens / Q000378:metabolism*; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry / Q000378:metabolism*; D007700:Kinetics; D050356:Lipid Metabolism; D008460:Meat / Q000032:analysis*; D018482:Muscle, Skeletal / Q000737:chemistry / Q000201:enzymology* / Q000378:metabolism; D014422:Turkeys / Q000378:metabolism*; D014810:Vitamin E / Q000737:chemistry* / Q000378:metabolism
chemical_list: D014810:Vitamin E; D003577:Cytochrome P-450 Enzyme System
keywords: lipid oxidation; metabolites; poultry; rancidity; vitamin E