title: Folic-Acid-Modified Conducting Polymer: Electrochemical Detection of the Cell Attachment.
abstract: Here, postfunctionalization and bioapplication of a π-conjugated polymer named 4-[4H-dithieno(3,2-b:2',3'-d)pyrrol-4-yl]aniline (DTP-aryl-NH2 ) are reported, which is successfully synthesized via electropolymerization onto the glassy carbon electrode. Folic acid (FA) is used to modify the amino functional polymer via N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride/N-hydroxysuccinimide chemistry for the further steps. The selective adhesion of folate receptor positive cells on the surface is followed by the electrochemical methods. Cyclic voltammetry and electrochemical impedance spectroscopy have been used to characterize stepwise modification of the electroactive surface. After optimization studies such as scan rate during the polymer deposition, FA amount for the efficient surface targeting, incubation time with the cells etc., analytical characterization is carried out. The surface morphologies at each step are imaged by using fluorescence microscopy.
authors.affiliation: Kamil Ozdag Science Faculty, Chemistry Department, Karamanoglu Mehmetbey University, 70100, Karaman, Turkey.
authors.forename: Hacer
authors.initials: H
authors.lastname: Azak
authors.affiliation: Faculty of Science, Department of Biochemistry, Ege University, 35100, Izmir, Turkey.
authors.forename: Firat Baris
authors.initials: FB
authors.lastname: Barlas
authors.affiliation: Department of Materials Science and Nano Technology Engineering, KTO Karatay University, 42020, Konya, Turkey.
authors.forename: Huseyin Bekir
authors.initials: HB
authors.lastname: Yildiz
authors.affiliation: Graduate School of Natural and Applied Sciences, Biotechnology Department, Ege University, 35100, Izmir, Turkey.
authors.forename: Kadri
authors.initials: K
authors.lastname: Gulec
authors.affiliation: Faculty of Science, Department of Biochemistry, Ege University, 35100, Izmir, Turkey.
authors.forename: Bilal
authors.initials: B
authors.lastname: Demir
authors.affiliation: Faculty of Science, Department of Biochemistry, Ege University, 35100, Izmir, Turkey.
authors.forename: Dilek Odaci
authors.initials: DO
authors.lastname: Demirkol
authors.affiliation: Faculty of Science, Department of Biochemistry, Ege University, 35100, Izmir, Turkey.
authors.forename: Suna
authors.initials: S
authors.lastname: Timur
mesh_terms: D000072283:A549 Cells; D000814:Aniline Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D002234:Carbodiimides / Q000737:chemistry*; D002244:Carbon; D002448:Cell Adhesion; D055664:Electrochemical Techniques; D004566:Electrodes; D058975:Folate Receptor 1 / Q000235:genetics / Q000378:metabolism; D005492:Folic Acid / Q000737:chemistry* / Q000378:metabolism; D015870:Gene Expression; D005898:Glass; D006367:HeLa Cells; D006801:Humans; D061986:MCF-7 Cells; D008744:Methylamines / Q000737:chemistry*; D058105:Polymerization; D011485:Protein Binding; D013388:Succinimides / Q000737:chemistry*
chemical_list: C569779:1-(3-dimethylaminopropyl)-dimethylaminopropyl-ethylcarbodiimide hydrochloride; D000814:Aniline Compounds; D002234:Carbodiimides; D058975:Folate Receptor 1; D008744:Methylamines; D013388:Succinimides; D002244:Carbon; D005492:Folic Acid; C001426:N-hydroxysuccinimide
keywords: cell adhesion; cell detection; conducting polymer; surface modification