title: The effect of initial periodontal treatment on plasma, gingival crevicular fluid and salivary levels of 8-hydroxy-deoxyguanosine in obesity.
abstract: OBJECTIVE
  Recent studies have shown adverse effects on the periodontium from the increased production of reactive oxygen species (ROS) in obesity. The purpose of this study was to investigate the effects of obesity on 8-hydroxy-deoxyguanosine (8-OHdG) levels in the bodily fluids of patients with and without periodontal disease and to evaluate changes after initial periodontal treatment.
  
  
  METHODS
  Forty-five obese individuals and 45 normal-weight individuals were included in this study. Obese and normal-weight groups were classified into three sub-groups: chronic periodontitis (CP), gingivitis (G) and periodontally healthy controls (CTRL). Gingival crevicular fluid (GCF), plasma, saliva samples and clinical measurements were obtained at baseline and a month after initial periodontal treatment. Levels of 8-OHdG were analysed by ELISA.
  
  
  RESULTS
  While plasma 8-OHdG levels were significantly higher at baseline in the obese patients with periodontal disease than in the normal-weight individuals (P<0.05), no significant differences in GCF and saliva 8-OHdG levels were found (P ˃ 0.05). GCF and salivary 8-OHdG levels in obese patients with G and CP were significantly higher than in CTRL groups at baseline (P<0.05). After treatment, 8-OHdG levels were decreased in all groups with periodontal disease (P<0.01). Statistically significant positive correlations were observed between GCF 8-OHdG levels and GI in all the groups (P<0.001).
  
  
  CONCLUSIONS
  The significant increase of plasma 8-OHdG levels in obese patients did not correlate with saliva and GCF 8-OHdG levels when compared to normal-weight individuals. Periodontal treatment had a positive effect on the periodontal parameters and 8-OHdG levels of both obese and normal-weight individuals.
authors.affiliation: Department of Periodontology, Faculty of Dentistry, Bülent Ecevit University, Zonguldak, Turkey. Electronic address: figen_ongoz@hotmail.com.
authors.forename: Figen
authors.initials: F
authors.lastname: Öngöz Dede
authors.affiliation: Department of Periodontology, Faculty of Dentistry, Bülent Ecevit University, Zonguldak, Turkey.
authors.forename: Şeyma
authors.initials: Ş
authors.lastname: Bozkurt Doğan
authors.affiliation: Department of Periodontology, Faculty of Dentistry, Bülent Ecevit University, Zonguldak, Turkey.
authors.forename: Umut
authors.initials: U
authors.lastname: Ballı
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Ondokuzmayis University, Samsun, Turkey.
authors.forename: Bahattin
authors.initials: B
authors.lastname: Avcı
authors.affiliation: Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Bülent Ecevit University, Zonguldak, Turkey.
authors.forename: Muştafa Cenk
authors.initials: MC
authors.lastname: Durmuşlar
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D016022:Case-Control Studies; D055113:Chronic Periodontitis / Q000097:blood / Q000378:metabolism* / Q000628:therapy; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000378:metabolism; D005260:Female; D005883:Gingival Crevicular Fluid / Q000187:drug effects / Q000378:metabolism*; D005891:Gingivitis / Q000097:blood / Q000378:metabolism / Q000628:therapy; D006801:Humans; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000097:blood / Q000378:metabolism*; D018384:Oxidative Stress; D017622:Periodontal Attachment Loss / Q000378:metabolism / Q000628:therapy; D017382:Reactive Oxygen Species / Q000378:metabolism; D012463:Saliva / Q000737:chemistry / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D017382:Reactive Oxygen Species; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine
keywords: 8-Hydroxy-deoxyguanosine; Obesity; Periodontal disease; Periodontal treatment