title: Transcriptome Sequencing for the Detection of Chimeric Transcripts.
abstract: The occurrence of chimeric transcripts has been reported in many cancer cells and seen as potential biomarkers and therapeutic targets. Modern high-throughput sequencing technologies offer a way to investigate individual chimeric transcripts and the systematic information of associated gene expressions about underlying genome structural variations and genomic interactions. The detection methods of finding chimeric transcripts from massive amount of short read sequence data are discussed here. Both assembly-based and alignment-based methods are used for the investigation of chimeric transcripts.
authors.affiliation: Department of Computer Science and Information Engineering, Asia University, No. 500, Liufeng Road, Wufeng District, Taichung City, 413, Taiwan. htchu@asia.edu.tw.|Department of Medical Research, China Medical University Hospital, China Medical University, Taichung, Taiwan. htchu@asia.edu.tw.
authors.forename: Hsueh-Ting
authors.initials: HT
authors.lastname: Chu
mesh_terms: D020869:Gene Expression Profiling / Q000379:methods*; D050939:Gene Fusion; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods; D006801:Humans; D009369:Neoplasms / Q000235:genetics; D012313:RNA / Q000235:genetics; D059467:Transcriptome
chemical_list: D012313:RNA
keywords: Cancer genes; Chimeric transcript; De novo assembly; Fusion genes; Transcriptome sequencing