title: High-Frequency, Low-Intensity Pulsed Ultrasound Enhances Alveolar Bone Healing of Extraction Sockets in Rats: A Pilot Study.
abstract: Most studies of the beneficial effects of low-intensity pulsed ultrasound (LIPUS) on bone healing have used frequencies between 1.0 and 1.5 MHz. However, after consideration of ultrasound wave characteristics and depth of target tissue, higher-frequency LIPUS may have been more effective on superficially positioned alveolar bone. We investigated this hypothesis by applying LIPUS (frequency, 3.0 MHz; intensity, 30 mW/cm(2)) on shaved right cheeks over alveolar bones of tooth extraction sockets in rats for 10 min/d for 2 wk after tooth extraction; the control group (left cheek of the same rats) did not receive LIPUS treatment. Compared with the control group, the LIPUS group manifested more new bone growth inside the sockets on histomorphometric analysis (maximal difference = 2.5-fold on the seventh day after extraction) and higher expressions of osteogenesis-related mRNAs and proteins than the control group did. These findings indicate that 3.0-MHz LIPUS could enhance alveolar bone formation and calcification in rats.
authors.affiliation: Department of Periodontology, School of Dentistry, Kyung Hee University, Seoul, Korea; Institute of Oral Biology, School of Dentistry, Kyung Hee University, Seoul, Korea. Electronic address: periokkl@khu.ac.kr.
authors.forename: Kyung Lhi
authors.initials: KL
authors.lastname: Kang
authors.affiliation: Institute of Oral Biology, School of Dentistry, Kyung Hee University, Seoul, Korea; Department of Oral and Maxillofacial Pathology, School of Dentistry and Research Center for Tooth & Periodontal Tissue Regeneration (MRC), Kyung Hee University, Seoul, Korea.
authors.forename: Eun-Cheol
authors.initials: EC
authors.lastname: Kim
authors.affiliation: Department of Periodontology, School of Dentistry, Kyung Hee University, Seoul, Korea; Institute of Oral Biology, School of Dentistry, Kyung Hee University, Seoul, Korea.
authors.forename: Joon Bong
authors.initials: JB
authors.lastname: Park
authors.affiliation: Institute of Oral Biology, School of Dentistry, Kyung Hee University, Seoul, Korea; Department of Maxillofacial Biomedical Engineering, School of Dentistry, Kyung Hee University, Seoul, Korea.
authors.forename: Jung Sun
authors.initials: JS
authors.lastname: Heo
authors.affiliation: Department of Periodontology, School of Dentistry, Kyung Hee University, Seoul, Korea.
authors.forename: Yumi
authors.initials: Y
authors.lastname: Choi
mesh_terms: D000818:Animals; D001861:Bone Regeneration / Q000528:radiation effects*; D004305:Dose-Response Relationship, Drug; D008297:Male; D008963:Molar / Q000473:pathology / Q000528:radiation effects / Q000601:surgery*; D010865:Pilot Projects; D011829:Radiation Dosage; D051381:Rats; D017207:Rats, Sprague-Dawley; D014081:Tooth Extraction; D020390:Tooth Socket / Q000254:growth & development* / Q000473:pathology / Q000528:radiation effects*; D016896:Treatment Outcome; D014464:Ultrasonic Therapy / Q000379:methods*; D000069453:Ultrasonic Waves
keywords: Alveolar bone formation in rats; High frequency; Low-intensity pulsed ultrasound; Tooth extraction socket healing