title: Comparative transcriptome analysis of grapevine in response to copper stress.
abstract: Grapevine is one of the most economically important and widely cultivated fruit crop worldwide. With the industrialization and the popular application of cupric fungicides in grape industry, copper stress and copper pollution are also the factors affecting grape production and berry and wine quality. Here, 3,843 transcripts were significantly differently expressed genes in response to Cu stress by RNA-seq, which included 1,892 up-regulated and 1,951 down-regulated transcripts. During this study we found many known and novel Cu-induced and -repressed genes. Biological analysis of grape samples were indicated that exogenous Cu can influence chlorophylls metabolism and photosynthetic activities of grapevine. Most ROS detoxification systems, including antioxidant enzyme, stress-related proteins and secondary metabolites were strongly induced. Concomitantly, abscisic acid functioned as a negative regulator in Cu stress, in opposite action to ethylene, auxin, jasmonic acid, and brassinolide. This study also identified a set of Cu stress specifically activated genes coding copper transporter, P1B-type ATPase, multidrug transporters. Overall, this work was carried out to gain insights into the copper-regulated and stress-responsive mechanisms in grapevine at transcriptome level. This research can also provide some genetic information that can help us in better vinery management and breeding Cu-resistant grape cultivars.
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Tongwei Road 6, Nanjing 210095, PR. China.
authors.forename: Xiangpeng
authors.initials: X
authors.lastname: Leng
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Tongwei Road 6, Nanjing 210095, PR. China.
authors.forename: Haifeng
authors.initials: H
authors.lastname: Jia
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Tongwei Road 6, Nanjing 210095, PR. China.
authors.forename: Xin
authors.initials: X
authors.lastname: Sun
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Tongwei Road 6, Nanjing 210095, PR. China.
authors.forename: Lingfei
authors.initials: L
authors.lastname: Shangguan
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Tongwei Road 6, Nanjing 210095, PR. China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Mu
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Tongwei Road 6, Nanjing 210095, PR. China.
authors.forename: Baoju
authors.initials: B
authors.lastname: Wang
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Tongwei Road 6, Nanjing 210095, PR. China.
authors.forename: Jinggui
authors.initials: J
authors.lastname: Fang
mesh_terms: D002734:Chlorophyll / Q000378:metabolism; D003300:Copper / Q000494:pharmacology*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D063990:Gene Ontology; D017343:Genes, Plant / Q000235:genetics; D010788:Photosynthesis / Q000235:genetics; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000235:genetics; D059467:Transcriptome / Q000187:drug effects*; D027843:Vitis / Q000235:genetics* / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D017382:Reactive Oxygen Species; D002734:Chlorophyll; D003300:Copper