title: Salinicola rhizosphaeraesp. nov., isolated from the rhizosphere of the mangroveAvicennia marinaL.
abstract: A Gram-stain-negative, motile, rod-shaped bacterium, designated MSSRFH1T, was isolated from the rhizosphere of the mangrove,Avicennia marina, in Pichavaram, Tamil Nadu, India. Phylogenetic analysis, based on the 16S rRNA gene sequence of MSSRFH1T, indicated that it clustered in the genusSalinicolaand was most closely related toSalinicola peritrichatusJCM18795T(96.7 % similarity). The 16S rRNA gene sequence similarity was < 96.5 % with other strains of species of the genusSalinicola. The distinctiveness of strain MSSRFH1Twas also shown by low similarities of itsrpoD( < 87 % similarity) andgyrB( < 85 %) gene sequences with those of other members of the genusSalinicola. Strain MSSRFH1Tcould tolerate NaCl concentrations of up to 30 % (w/v). The main fatty acids of MSSRFH1Tincluded C18 : 1ω7c, C16 : 0and C19 : 0ω8c. The polar lipids present included diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, an unidentified amino lipid and unidentified phospholipids. Comparison of BOX-PCR fingerprints indicated that MSSRFH1Tshowed a unique DNA profile and its genomic G+C content was 64 mol%. On the basis of the data presented, strain MSSRFH1Trepresents a novel species of the genusSalinicola,for which the nameSalinicola rhizosphaeraesp. nov. is proposed. The type strain is MSSRFH1T( = KCTC 32998T= NBRC 110222T).
authors.affiliation: Microbiology Department, M.S. Swaminathan Research Foundation, 3rd Cross Street, Taramani Institutional Area, Chennai 600113, India.
authors.forename: Kathiravan
authors.initials: K
authors.lastname: Raju
authors.affiliation: Microbiology Department, M.S. Swaminathan Research Foundation, 3rd Cross Street, Taramani Institutional Area, Chennai 600113, India.
authors.forename: Jegan
authors.initials: J
authors.lastname: Sekar
authors.affiliation: Microbiology Department, M.S. Swaminathan Research Foundation, 3rd Cross Street, Taramani Institutional Area, Chennai 600113, India.
authors.forename: Prabavathy
authors.initials: P
authors.lastname: Vaiyapuri Ramalingam