title: Myocardial Ischemic Subject's Thymus Fat: A Novel Source of Multipotent Stromal Cells.
abstract: OBJECTIVE
  Adipose Tissue Stromal Cells (ASCs) have important clinical applications in the regenerative medicine, cell replacement and gene therapies. Subcutaneous Adipose Tissue (SAT) is the most common source of these cells. The adult human thymus degenerates into adipose tissue (TAT). However, it has never been studied before as a source of stem cells.
  
  
  METHODS
  We performed a comparative characterization of TAT-ASCs and SAT-ASCs from myocardial ischemic subjects (n = 32) according to the age of the subjects.
  
  
  RESULTS
  TAT-ASCs and SAT-ASCs showed similar features regarding their adherence, morphology and in their capacity to form CFU-F. Moreover, they have the capacity to differentiate into osteocyte and adipocyte lineages; and they present a surface marker profile corresponding with stem cells derived from AT; CD73+CD90+CD105+CD14-CD19-CD45-HLA-DR. Interestingly, and in opposition to SAT-ASCs, TAT-ASCs have CD14+CD34+CD133+CD45- cells. Moreover, TAT-ASCs from elderly subjects showed higher adipogenic and osteogenic capacities compared to middle aged subjects, indicating that, rather than impairing; aging seems to increase adipogenic and osteogenic capacities of TAT-ASCs.
  
  
  CONCLUSIONS
  This study describes the human TAT as a source of mesenchymal stem cells, which may have an enormous potential for regenerative medicine.
authors.affiliation: CIBER Pathophysiology of obesity and nutrition CB06/03, Carlos III Health Institute, Malaga, 29010, Spain, Laboratory of Biomedical Research, Virgen de la Victoria Clinical University Hospital, Málaga, 29010, Spain.
authors.forename: Wilfredo
authors.initials: W
authors.lastname: Oliva-Olivera
authors.affiliation: CIBER Pathophysiology of obesity and nutrition CB06/03, Carlos III Health Institute, Malaga, 29010, Spain, Laboratory of Biomedical Research, Virgen de la Victoria Clinical University Hospital, Málaga, 29010, Spain.
authors.forename: Leticia
authors.initials: L
authors.lastname: Coín-Aragüez
authors.affiliation: Cardiovascular Surgery Department, University Hospital Carlos Haya, Málaga, Spain.
authors.forename: Julián
authors.initials: J
authors.lastname: Salas
authors.affiliation: Research Laboratory, Science School, Málaga University, Campus Teatinos s/n- 29071, Málaga, Spain.
authors.forename: Said
authors.initials: S
authors.lastname: Lhamyani
authors.affiliation: Research Laboratory, Science School, Málaga University, Campus Teatinos s/n- 29071, Málaga, Spain.
authors.forename: Adriana-Mariel
authors.initials: AM
authors.lastname: Gentile
authors.affiliation: Cardiovascular Surgery Department, University Hospital Carlos Haya, Málaga, Spain.
authors.forename: Esteban
authors.initials: E
authors.lastname: Sarria García
authors.affiliation: Andalusian Center for Molecular Biology and Regenerative Medicine (CABIMER), Department of Stem Cells, Sevilla, Spain, Centro de Investigación Biomédica en Red-Diabetes y Enfermedades Metabólicas (CIBERDEM), Instituto de Salud Carlos III, Madrid, Spain.
authors.forename: Abdelkrim
authors.initials: A
authors.lastname: Hmadcha
authors.affiliation: Biomedical Sciences Program, Health Sciences Department, College of Arts and Sciences, Qatar University, P.O. Box: 2713, Doha, Qatar.
authors.forename: Hatem
authors.initials: H
authors.lastname: Zayed
authors.affiliation: Servicio Regional de Inmunología y Alergia, Hospital Universitario Virgen Macarena, Sevilla, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Vega-Rioja
authors.affiliation: CIBER Pathophysiology of obesity and nutrition CB06/03, Carlos III Health Institute, Malaga, 29010, Spain, Laboratory of Biomedical Research, Virgen de la Victoria Clinical University Hospital, Málaga, 29010, Spain.
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Tinahones
authors.affiliation: CIBER Pathophysiology of obesity and nutrition CB06/03, Carlos III Health Institute, Malaga, 29010, Spain, Laboratory of Biomedical Research, Virgen de la Victoria Clinical University Hospital, Málaga, 29010, Spain.
authors.forename: Rajaa
authors.initials: R
authors.lastname: El Bekay
mesh_terms: D050156:Adipogenesis; D000273:Adipose Tissue / Q000473:pathology*; D000368:Aged; D002454:Cell Differentiation; D049109:Cell Proliferation; D003114:Colony-Forming Units Assay; D050556:Fatty Acid-Binding Proteins / Q000235:genetics; D005434:Flow Cytometry; D015870:Gene Expression; D006801:Humans; D016130:Immunophenotyping; D059630:Mesenchymal Stem Cells / Q000378:metabolism / Q000473:pathology*; D008875:Middle Aged; D017202:Myocardial Ischemia / Q000473:pathology*; D010012:Osteogenesis; D044968:Regenerative Medicine / Q000379:methods; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051780:Sterol Regulatory Element Binding Protein 1 / Q000235:genetics; D050151:Subcutaneous Fat / Q000378:metabolism / Q000473:pathology; D013950:Thymus Gland / Q000473:pathology*
chemical_list: C493410:FABP4 protein, human; D050556:Fatty Acid-Binding Proteins; C497387:SREBF1 protein, human; D051780:Sterol Regulatory Element Binding Protein 1