title: [Cloning and Bioinformatics Analysis of Toxoplasma gondii ROP21 Gene].
abstract: The full-length gene sequence of Toxoplasma gondii ROP21 (TgROP21) gene was amplified with PCR. The signaling peptide and transmembrane domain of TgROP21 protein were predicted by SignaIP and TMHMM online predictive sites, and the hydrophilicity and antigenic index of this protein were ananlyzed with DNAStar software. Meanwhile, the functional domains and tertiary structure were modeled by combined use of ExPASY and PRODATA online sites. As expected, the PCR results revealed one band at 2,022 bp. The signaling peptide, transmembrane domain, hydrophilicity, antigen index, functional domain and 3D structure of TgROP21 were successfully predicted. This work may provide a theoretical foundation for further verification of TgROP21 function.
authors.forename: Shi-jun
authors.initials: SJ
authors.lastname: Shi
authors.forename: Yong
authors.initials: Y
authors.lastname: Cui
authors.forename: Jin
authors.initials: J
authors.lastname: Li
authors.forename: Hong-fa
authors.initials: HF
authors.lastname: Wang
authors.forename: Kun
authors.initials: K
authors.lastname: Yin
authors.forename: Qing-kuan
authors.initials: QK
authors.lastname: Wei
authors.forename: Bing-cheng
authors.initials: BC
authors.lastname: Huang
authors.forename: Hui
authors.initials: H
authors.lastname: Sun
authors.forename: Gong-zhen
authors.initials: GZ
authors.lastname: Liu
mesh_terms: D003001:Cloning, Molecular; D019295:Computational Biology; D017125:Genes, Protozoan; D016133:Polymerase Chain Reaction; D014122:Toxoplasma