title: [Molecular structural characteristics and the functions of mouse DNA-dependent activator of interferon-regulatory factors].
abstract: OBJECTIVE
  To study the structural characteristics of the mouse DNA-dependent activator of interferon-regulatory factors (DAI) and its related molecular mechanism in anti-viral innate immune responses and signal transduction.
  
  
  METHODS
  The coding sequence of mouse DAI gene was amplified from splenic mononuclear cells by reverse transcription-PCR, and the genetic evolution and molecular structure of the mouse DAI gene were analyzed by bioinformatics softwares. After mouse DAI was stimulated by poly(dA-dT) and poly(dG-dC), the nuclear factor κB (NF-κB) and interferon-beta (IFN-β) promoter-driven luciferase activity were detected by dual-luciferase reporter assay system.
  
  
  RESULTS
  The open reading frame (ORF) of the cloned mouse DAI sequence was 1236 bp, encoding 411 amino acids, which exhibited identity with the corresponding sequences of cattle, pig, rat and other mammals ranging from 60%, 63.1%, 84%, and it contained two Z-DNA domains (Zα and Zβ), DNA binding region (D3) and signaling domain (SD). The stimulation of poly (dA-dT) increased the expressions of mouse DAI activated transcription factors NF-κB and IFN-β promoter. However, the stimulation of poly(dG-dC) only induced the activation of NF-κB but not IFN-β promoter.
  
  
  CONCLUSIONS
  Mouse DAI as an important cytosolic DNA sensor, is responsible for the recognition of A/T or G/C-rich DNA derived from DNA virus. It may play an important role in anti-viral innate immune responses.
authors.affiliation: College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070; State Key Laboratory of Veterinary Etiological Biology, Ministry of Agriculture Key Laboratory of Veterinary Public Health, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.
authors.forename: Qiwang
authors.initials: Q
authors.lastname: Jin
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Ministry of Agriculture Key Laboratory of Veterinary Public Health, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Li
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Ministry of Agriculture Key Laboratory of Veterinary Public Health, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.
authors.forename: Xiaobing
authors.initials: X
authors.lastname: He
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Ministry of Agriculture Key Laboratory of Veterinary Public Health, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.
authors.forename: Huaijie
authors.initials: H
authors.lastname: Jia
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Ministry of Agriculture Key Laboratory of Veterinary Public Health, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.
authors.forename: Guohua
authors.initials: G
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Ministry of Agriculture Key Laboratory of Veterinary Public Health, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Zeng
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Ministry of Agriculture Key Laboratory of Veterinary Public Health, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.
authors.forename: Yongxiang
authors.initials: Y
authors.lastname: Fang
authors.affiliation: [1]College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070; [2]State Key Laboratory of Veterinary Etiological Biology, Ministry of Agriculture Key Laboratory of Veterinary Public Health, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China. *Corresponding authors, E-mail: zhizhongj@163.com.
authors.forename: Zhizhong
authors.initials: Z
authors.lastname: Jing
authors.affiliation: College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China. *Corresponding authors, E-mail: yangxpu@gsau.edu.cn.
authors.forename: Xiaopu
authors.initials: X
authors.lastname: Yang
mesh_terms: D000818:Animals; D002417:Cattle; D004247:DNA / Q000378:metabolism*; D005260:Female; D006023:Glycoproteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D008297:Male; D008322:Mammals / Q000145:classification / Q000235:genetics; D051379:Mice / Q000235:genetics* / Q000378:metabolism; D008810:Mice, Inbred C57BL; D017434:Protein Structure, Tertiary; D016601:RNA-Binding Proteins; D051381:Rats; D013552:Swine
chemical_list: D006023:Glycoproteins; D016601:RNA-Binding Proteins; C402632:Zbp1 protein, mouse; D004247:DNA