title: Advantages of a dual-color fluorescence-tracing glioma orthotopic implantation model: Detecting tumor location, angiogenesis, cellular fusion and the tumor microenvironment.
abstract: Various organs of the body have distinct microenvironments with diverse biological characteristics that can influence the growth of tumors within them. However, the mechanisms underlying the interactions between tumor and host cells are currently not well understood. In the present study, a dual-color fluorescence-tracing glioma orthotopic implantation model was developed, in which C6 rat glioma cells labeled with the red fluorescent dye CM-Dil, and SU3 human glioma cells stably expressing red fluorescence protein, were inoculated into the right caudate nucleus of transgenic female C57BL/6 nude mice expressing enhanced green fluorescent protein. The dual-color tracing with whole-bodyin vivofluorescence imaging of xenografts was performed using a live imaging system. Frozen sections of the transplanted tumor were prepared for histological analyses, in order to detect the presence of invading tumor cells, blood vessels and cellular fusion. Dual-color images were able to distinguish between red tumor cells and green host cells. The results of the present study suggested that a dual-color fluorescence-tracing glioma orthotopic implantation model may be convenient for detecting tumor location, angiogenesis, cellular fusion, and the tumor microenvironment.
authors.affiliation: Department of Neurosurgery, Radiotherapy and Oncology, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, P.R. China.
authors.forename: Yuntian
authors.initials: Y
authors.lastname: Shen
authors.affiliation: Department of Neurosurgery, Shanghai Tenth People's Hospital, Shanghai 200072, P.R. China.
authors.forename: Quanbin
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Department of Neurosurgery, Ganzhou People's Hospital, Ganzhou, Jiangxi 341000, P.R. China.
authors.forename: Jinshi
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Neurosurgery, Radiotherapy and Oncology, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, P.R. China.
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Lu
authors.affiliation: Department of Neurosurgery, Radiotherapy and Oncology, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, P.R. China.
authors.forename: Aidong
authors.initials: A
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, Suzhou Kowloon Hospital, Suzhou, Jiangsu 215002, P.R. China.
authors.forename: Xifeng
authors.initials: X
authors.lastname: Fei
authors.affiliation: Department of Neurosurgery, Radiotherapy and Oncology, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, P.R. China.
authors.forename: Xingliang
authors.initials: X
authors.lastname: Dai
authors.affiliation: Department of Neurosurgery, Radiotherapy and Oncology, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, P.R. China.
authors.forename: Jinding
authors.initials: J
authors.lastname: Wu
authors.affiliation: Department of Neurosurgery, Suzhou Kowloon Hospital, Suzhou, Jiangsu 215002, P.R. China.
authors.forename: Zhimin
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, Shanghai Tenth People's Hospital, Shanghai 200072, P.R. China.
authors.forename: Yaodong
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Department of Neurosurgery, Radiotherapy and Oncology, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, P.R. China.
authors.forename: Y E
authors.initials: YE
authors.lastname: Tian
authors.affiliation: Department of Neurosurgery, Radiotherapy and Oncology, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, P.R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Dong
authors.affiliation: Department of Neurosurgery, Radiotherapy and Oncology, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, P.R. China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Lan
authors.affiliation: Department of Neurosurgery, Radiotherapy and Oncology, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, P.R. China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Huang
keywords: cellular fusion; dual-color fluorescence; fluorescence-tracing; glioma; orthotopic implantation model