title: Platelet Lysate-Modified Porous Silicon Microparticles for Enhanced Cell Proliferation in Wound Healing Applications.
abstract: The new frontier in the treatment of chronic nonhealing wounds is the use of micro- and nanoparticles to deliver drugs or growth factors into the wound. Here, we used platelet lysate (PL), a hemoderivative of platelets, consisting of a multifactorial cocktail of growth factors, to modify porous silicon (PSi) microparticles and assessed both in vitro and ex vivo the properties of the developed microsystem. PL-modified PSi was assessed for its potential to induce proliferation of fibroblasts. The wound closure-promoting properties of the microsystem were then assessed in an in vitro wound healing assay. Finally, the PL-modified PSi microparticles were evaluated in an ex vivo experiment over human skin. It was shown that PL-modified PSi microparticles were cytocompatible and enhanced the cell proliferation in different experimental settings. In addition, this microsystem promoted the closure of the gap between the fibroblast cells in the wound healing assay, in periods of time comparable with the positive control, and induced a proliferation and regeneration process onto the human skin in an ex vivo experiment. Overall, our results show that PL-modified PSi microparticles are suitable microsystems for further development toward applications in the treatment of chronic nonhealing wounds.
authors.affiliation: Division of Pharmaceutical Chemistry and Technology, Faculty of Pharmacy, University of Helsinki , Helsinki 00014, Finland.
authors.forename: Flavia
authors.initials: F
authors.lastname: Fontana
authors.forename: Michela
authors.initials: M
authors.lastname: Mori
authors.forename: Federica
authors.initials: F
authors.lastname: Riva
authors.affiliation: Laboratory of Industrial Physics, University of Turku , Turku, Finland.
authors.forename: Ermei
authors.initials: E
authors.lastname: Mäkilä
authors.affiliation: Division of Pharmaceutical Chemistry and Technology, Faculty of Pharmacy, University of Helsinki , Helsinki 00014, Finland.
authors.forename: Dongfei
authors.initials: D
authors.lastname: Liu
authors.affiliation: Laboratory of Industrial Physics, University of Turku , Turku, Finland.
authors.forename: Jarno
authors.initials: J
authors.lastname: Salonen
authors.forename: Giovanni
authors.initials: G
authors.lastname: Nicoletti
authors.affiliation: Division of Pharmaceutical Chemistry and Technology, Faculty of Pharmacy, University of Helsinki , Helsinki 00014, Finland.
authors.forename: Jouni
authors.initials: J
authors.lastname: Hirvonen
authors.forename: Carla
authors.initials: C
authors.lastname: Caramella
authors.affiliation: Division of Pharmaceutical Chemistry and Technology, Faculty of Pharmacy, University of Helsinki , Helsinki 00014, Finland.
authors.forename: Hélder A
authors.initials: HA
authors.lastname: Santos
mesh_terms: D000328:Adult; D001692:Biological Transport / Q000187:drug effects; D001792:Blood Platelets / Q000378:metabolism*; D002452:Cell Count; D016923:Cell Death / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D006801:Humans; D008297:Male; D016062:Porosity; D011247:Pregnancy; D012825:Silicon / Q000494:pharmacology*; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D012825:Silicon
keywords: fibroblast; microparticles; platelet lysate; porous silicon; wound healing