title: Transcriptome changes in Polygonum multiflorum Thunb. roots induced by methyl jasmonate.
abstract: Transcriptome profiling has been widely used to analyze transcriptomic variation in plants subjected to abiotic or biotic stresses. Although gene expression changes induced by methyl jasmonate (MeJA) have been profiled in several plant species, no information is available on the MeJA-triggered transcriptome response of Polygonum multiflorum Thunb., a species with highly valuable medicinal properties. In this study, we used transcriptome profiling to investigate transcriptome changes in roots of P. multiflorum seedlings subjected to a 0.25 mmol/L-MeJA root-irrigation treatment. A total of 18 677 differentially expressed genes (DEGs) were induced by MeJA treatment, of which 4535 were up-regulated and 14 142 were down-regulated compared with controls. These DEGs were associated with 125 metabolic pathways. In addition to various common primary and secondary metabolic pathways, several secondary metabolic pathways related to components with significant pharmacological effects were enriched by MeJA, including arachidonic acid metabolism, linoleic acid metabolism, and stilbenoid biosynthesis. The MeJA-induced transcriptome changes uncovered in this study provide a solid foundation for future study of functional genes controlling effective components in secondary metabolic pathways of P. multiflorum.
authors.affiliation: College of Agronomy, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Hong-chang
authors.initials: HC
authors.lastname: Liu
authors.affiliation: College of Agronomy, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wu
authors.affiliation: College of Agronomy, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Hou
authors.affiliation: College of Agronomy, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Jun-wen
authors.initials: JW
authors.lastname: Chen
authors.affiliation: Guizhou Key (Engineering) Laboratory for Propagation and Cultivation of Medicinal Plants, Guiyang 550025, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Zhao
mesh_terms: D000085:Acetates / Q000494:pharmacology*; D016718:Arachidonic Acid / Q000378:metabolism; D003517:Cyclopentanes / Q000494:pharmacology*; D000068676:Fallopia multiflora / Q000187:drug effects* / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D018745:Genome, Plant / Q000187:drug effects; D019787:Linoleic Acid / Q000378:metabolism; D053858:Metabolic Networks and Pathways / Q000187:drug effects / Q000235:genetics; D054883:Oxylipins / Q000494:pharmacology*; D010937:Plant Growth Regulators / Q000494:pharmacology*; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics; D013267:Stilbenes / Q000378:metabolism; D059467:Transcriptome / Q000187:drug effects*
chemical_list: D000085:Acetates; D003517:Cyclopentanes; D054883:Oxylipins; D010937:Plant Growth Regulators; D013267:Stilbenes; D016718:Arachidonic Acid; C072239:methyl jasmonate; D019787:Linoleic Acid
keywords: Differentially expressed genes; Methyl jasmonate; Polygonum multiflorum Thunb.; Transcriptome change