title: 9,10-Dihydrophenanthrene derivatives and one 1,4-anthraquinone firstly isolated from Dioscorea zingiberensis C. H. Wright and their biological activities.
abstract: Two new phenanthrene derivatives, 2,5,7-trimethoxy-9,10-dihydrophenanthrene-1,4-dione (1) and 2,5,6-trihydroxy-3,4-dimethoxy-9,10-dihydrophenanthrene (3), one new anthracenedione, 2,5,7-trimethoxyanthracene-1,4-dione (2), together with two known 9,10-dihydrophenanthrenes (4-5) were isolated from the rhizomes of Dioscorea zingiberensis C. H. Wright. The structures of these new compounds were established based on extensive NMR spectroscopy. Several isolated compounds were evaluated for the inhibition against nitric oxide (NO) production in the lipopolysaccharide (LPS)-activated RAW 264.7 macrophage cell line, DPPH radical scavenging, and inhibitory activity on Free Fatty Acids (FFAs) induced triglyceride accumulation in HepG2 cells. Compound 2 exhibited moderate anti-inflammatory activity, compound 3 possessed comparable DPPH radical scavenging activity as Vitamin C, compounds 2 and 4 showed potent inhibitory activities on triglyceride accumulation.
authors.affiliation: Laboratory of Ethnopharmacology, West China Hospital/West China Medical School, Sichuan University, Chengdu 610041, China. Electronic address: dudan1520@163.com.
authors.forename: Dan
authors.initials: D
authors.lastname: Du
authors.affiliation: Laboratory of Ethnopharmacology, West China Hospital/West China Medical School, Sichuan University, Chengdu 610041, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Laboratory of Ethnopharmacology, West China Hospital/West China Medical School, Sichuan University, Chengdu 610041, China.
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Xing
authors.affiliation: Laboratory of Ethnopharmacology, West China Hospital/West China Medical School, Sichuan University, Chengdu 610041, China; Department of Integrated Traditional Chinese and Western Medicine, West China Hospital/West China Medical School, Sichuan University, Chengdu 610041, China.
authors.forename: Yuyan
authors.initials: Y
authors.lastname: Liang
authors.affiliation: Key Laboratory of Transplant Engineering and Immunology, Ministry of Health, West China Hospital/West China Medical School, Sichuan University, Chengdu 610041, China.
authors.forename: Shengfu
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Integrated Traditional Chinese and Western Medicine, West China Hospital/West China Medical School, Sichuan University, Chengdu 610041, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Jin
authors.affiliation: Department of Integrated Traditional Chinese and Western Medicine, West China Hospital/West China Medical School, Sichuan University, Chengdu 610041, China. Electronic address: xiaqing@medmail.com.cn.
authors.forename: Qing
authors.initials: Q
authors.lastname: Xia
authors.affiliation: Key Laboratory of Transplant Engineering and Immunology, Ministry of Health, West China Hospital/West China Medical School, Sichuan University, Chengdu 610041, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Long
authors.affiliation: Laboratory of Ethnopharmacology, West China Hospital/West China Medical School, Sichuan University, Chengdu 610041, China.
authors.forename: Guang
authors.initials: G
authors.lastname: Xin
authors.affiliation: School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China.
authors.forename: Guangzhi
authors.initials: G
authors.lastname: Wang
authors.affiliation: Laboratory of Ethnopharmacology, West China Hospital/West China Medical School, Sichuan University, Chengdu 610041, China. Electronic address: huangwen@scu.edu.cn.
authors.forename: Wen
authors.initials: W
authors.lastname: Huang
mesh_terms: D000818:Animals; D000880:Anthraquinones / Q000737:chemistry* / Q000302:isolation & purification; D000893:Anti-Inflammatory Agents / Q000737:chemistry* / Q000302:isolation & purification; D027742:Dioscorea / Q000737:chemistry*; D016166:Free Radical Scavengers / Q000737:chemistry* / Q000302:isolation & purification; D056945:Hep G2 Cells; D006801:Humans; D008264:Macrophages / Q000187:drug effects; D051379:Mice; D015394:Molecular Structure; D009569:Nitric Oxide / Q000378:metabolism; D010616:Phenanthrenes / Q000737:chemistry* / Q000302:isolation & purification; D000067996:RAW 264.7 Cells; D027343:Rhizome / Q000737:chemistry; D014280:Triglycerides / Q000378:metabolism
chemical_list: D000880:Anthraquinones; D000893:Anti-Inflammatory Agents; D016166:Free Radical Scavengers; D010616:Phenanthrenes; D014280:Triglycerides; D009569:Nitric Oxide; C416442:1,4-anthraquinone
keywords: Anthracenedione; Anti-inflammatory; DPPH radical scavenging; Dihydrophenanthrene; Dioscorea zingiberensis C. H. Wright; Triglyceride accumulation