title: Antitumor Effect of C21 Steroidal Glycosides on Adenoid Cystic Carcinoma Cell Line SACC83.
abstract: BACKGROUND
  To observe the anti-tumor effects and possible mechanism of C21 steroidal glycoside (17-β-Marsdenia tenacissima glycoside B (17-β-tenacigenin B)) and Xiaoaiping injection in the adenoid cystic carcinoma cell line SACC83.
  
  
  METHODS
  We used the salivary adenoid cystic carcinoma cell line SACC83 for the study and used MTT, flow cytometry, and Western-blot (WB) to study the anti-tumor efficacy and mechanism of 17-β-tenacigenin B and Xiaoaiping injection. Three groups were divided as follows: group 1: control group (without drug treatment), group 2: SACC83 treated with 17-β-Marsdenia tenacissima glycoside B, group 3: SACC83 treated with Xiaoaiping injec- tion.
  
  
  RESULTS
  The 17-β-tenacigenin B had no significant anti-tumor effect while the Xiaoaiping injection had a dosage-dependent growth inhibition effect on the adenoid cystic carcinoma cell line SACC83. According to WB, after the administration of the 17-p-tenacigenin B, the expression of apoptosis-related protein was not very significant; however, after the administration of the Xiaoaiping injection, the expression of apoptosis inhibition protein bcl-2 decreased while the expression of apoptosis promotion protein bax increased.
  
  
  CONCLUSIONS
  The 17-β-tenacigenin B had no obvious anti-tumor effects while the Xiaoaiping injection had good anti-tumor effects. Even though the 17-β-tenacigenin B is an important component of the Xiaoaiping injection, in the in vitro experiment, compared with the Xiaoaiping injection, the 17-β-tenacigenin B showed no good anti-tumor effects. However, we need further studies on in vivo experiments of 17-β-tenacigenin B and the anti-tumor effect of other C21 steroidal glycoside monomers in the Xiaoaiping injection.
authors.forename: Qingliang
authors.initials: Q
authors.lastname: Wang
authors.forename: Jun
authors.initials: J
authors.lastname: Cao
authors.forename: Peng
authors.initials: P
authors.lastname: Wang
authors.forename: Minghua
authors.initials: M
authors.lastname: Ge
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis; D003528:Carcinoma, Adenoid Cystic / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D005434:Flow Cytometry; D006027:Glycosides / Q000494:pharmacology*; D006801:Humans; D031101:Marsdenia / Q000737:chemistry; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D012468:Salivary Gland Neoplasms / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D013256:Steroids / Q000627:therapeutic use*; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; C494826:BAX protein, human; C000595707:BCL2 protein, human; D006027:Glycosides; D019253:Proto-Oncogene Proteins c-bcl-2; D013256:Steroids; D051028:bcl-2-Associated X Protein; C531264:tenacigenin B