title: Prognostic role of microRNA-100 in patients with bladder cancer.
abstract: We investigated the clinical significance and prognostic value of microRNA-100 (miR-100) in bladder cancer. Quantitative real-time polymerase chain reaction was used to analyze the expression of miR-100 in 92 pairs of human bladder cancer and adjacent normal tissue samples. Overall survival (OS) curves were plotted using the Kaplan-Meier method and were evaluated for statistical significance using a log-rank test. The significance of different variables with respect to survival was analyzed using the multivariate Cox proportional hazard model. The miR-100 expression level was significantly lower in bladder cancer tissues than in normal adjacent tissues (mean ± SD: 1.49 ± 0.52 vs 2.79 ± 0.59, P < 0.05). A low miR-100 expression level was correlated with tumor stage (P = 0.023), tumor grade (P = 0.031), and regional lymph node involvement (P = 0.16). Kaplan-Meier analysis with log-rank test indicated that low miR-100 expression had a significant impact on OS (35.1 vs 75.3%; P = 0.004). Multivariate analysis revealed that the miR-100 expression level was an independent prognostic factor for OS (HR = 2.768, 95%CI = 1.287-8.992; P = 0.009) in bladder cancer patients. The present study demonstrated that the downregulation of miR-100 was associated with advanced clinical features and poor prognosis for bladder cancer patients, suggesting that miR-100 downregulation may be used as an unfavorable prognostic biomarker in bladder cancer.
authors.affiliation: Department of Urology, First Affiliated Hospital of Nahua University, Hengyang, China.
authors.forename: Y H
authors.initials: YH
authors.lastname: Cao
authors.affiliation: Department of Urology, First Affiliated Hospital of Nahua University, Hengyang, China.
authors.forename: H H
authors.initials: HH
authors.lastname: Zhang
authors.affiliation: Department of Urology, First Affiliated Hospital of Nahua University, Hengyang, China.
authors.forename: H F
authors.initials: HF
authors.lastname: Xu
authors.affiliation: Department of Urology, First Affiliated Hospital of Nahua University, Hengyang, China.
authors.forename: Y J
authors.initials: YJ
authors.lastname: Duan
authors.affiliation: Department of Urology, First Affiliated Hospital of Nahua University, Hengyang, China.
authors.forename: Q
authors.initials: Q
authors.lastname: Li
authors.affiliation: Department of Urology, First Affiliated Hospital of Nahua University, Hengyang, China.
authors.forename: B
authors.initials: B
authors.lastname: Huang
mesh_terms: D000328:Adult; D000368:Aged; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D060787:Neoplasm Grading; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging; D011379:Prognosis; D016016:Proportional Hazards Models; D060888:Real-Time Polymerase Chain Reaction; D047368:Tumor Burden; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000401:mortality* / Q000473:pathology / Q000628:therapy
chemical_list: C544266:MIRN100 microRNA, human; D035683:MicroRNAs