title: Spectroscopy and Fluorescence Lifetime Imaging Microscopy To Probe the Interaction of Bovine Serum Albumin with Graphene Oxide.
abstract: The interaction of graphene oxide (GO) with bovine serum albumin (BSA) in aqueous buffer solution has been investigated with various spectroscopic and imaging techniques. At single molecular resolution this interaction has been performed using fluorescence correlation spectroscopy (FCS) and fluorescence lifetime imaging microscopy (FLIM) techniques. The conformational dynamics of BSA on GO's influence have been explored by FCS and circular dichroism (CD) spectroscopy. For the FCS studies BSA was labeled covalently by a fluorophore, Alexa Fluor 488. On the addition of GO in phosphate buffer of 10 mM at pH 7.4 the diffusion time (τD) and the hydrodynamic radius (Rh) of BSA increase due to adsorption of BSA. Conformational relaxation time components of native BSA drastically vary with the addition of GO, signifying the change of conformational dynamics of BSA after addition of GO. The adsorption isotherm also indicates significant adsorption of BSA on the GO surface. Adsorption of BSA on the GO surface has shown in direct images of atomic force microscopy (AFM) and FLIM. Fluorescence quenching study of BSA with addition of GO also indicates that there is strong interaction between BSA and GO.
authors.affiliation: Department of Chemistry, Indian Institute of Technology , Kharagpur 721302, WB, India.
authors.forename: Jagannath
authors.initials: J
authors.lastname: Kuchlyan
authors.affiliation: Department of Chemistry, Indian Institute of Technology , Kharagpur 721302, WB, India.
authors.forename: Niloy
authors.initials: N
authors.lastname: Kundu
authors.affiliation: Department of Chemistry, Indian Institute of Technology , Kharagpur 721302, WB, India.
authors.forename: Debasis
authors.initials: D
authors.lastname: Banik
authors.affiliation: Department of Chemistry, Indian Institute of Technology , Kharagpur 721302, WB, India.
authors.forename: Arpita
authors.initials: A
authors.lastname: Roy
authors.affiliation: Department of Chemistry, Indian Institute of Technology , Kharagpur 721302, WB, India.
authors.forename: Nilmoni
authors.initials: N
authors.lastname: Sarkar
mesh_terms: D000818:Animals; D002417:Cattle; D002942:Circular Dichroism; D006108:Graphite / Q000737:chemistry*; D008856:Microscopy, Fluorescence; D015335:Molecular Probes / Q000737:chemistry*; D015394:Molecular Structure; D012235:Rhodamines / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013057:Spectrum Analysis; D013388:Succinimides / Q000737:chemistry
chemical_list: C492694:Alexa Fluor 488 carboxylic acid succinimidyl ester; D015335:Molecular Probes; D012235:Rhodamines; D013388:Succinimides; C026188:rhodamine 6G; D012710:Serum Albumin, Bovine; D006108:Graphite