title: Temporal Control of Membrane Fusion through Photolabile PEGylation of Liposome Membranes.
abstract: Membrane fusion results in the transport and mixing of (bio)molecules across otherwise impermeable barriers. In this communication, we describe the temporal control of targeted liposome-liposome membrane fusion and contents mixing using light as an external trigger. Our method relies on steric shielding and rapid, photoinduced deshielding of complementary fusogenic peptides tethered to opposing liposomal membranes. In an analogous approach, we were also able to demonstrate precise spatiotemporal control of liposome accumulation at cellular membranes in vitro.
authors.affiliation: Leiden Institute of Chemistry, Leiden University, P.O. Box 9052, 2300 RA, Leiden, The Netherlands.
authors.forename: Li
authors.initials: L
authors.lastname: Kong
authors.affiliation: Leiden Institute of Chemistry, Leiden University, P.O. Box 9052, 2300 RA, Leiden, The Netherlands.
authors.forename: Sven H C
authors.initials: SH
authors.lastname: Askes
authors.affiliation: Leiden Institute of Chemistry, Leiden University, P.O. Box 9052, 2300 RA, Leiden, The Netherlands.
authors.forename: Sylvestre
authors.identifier: http://orcid.org/0000-0002-5810-3657
authors.initials: S
authors.lastname: Bonnet
authors.affiliation: Leiden Institute of Chemistry, Leiden University, P.O. Box 9052, 2300 RA, Leiden, The Netherlands. a.kros@chem.leidenuniv.nl.
authors.forename: Alexander
authors.initials: A
authors.lastname: Kros
authors.affiliation: Leiden Institute of Chemistry, Leiden University, P.O. Box 9052, 2300 RA, Leiden, The Netherlands. f.campbell@chem.leidenuniv.nl.
authors.forename: Frederick
authors.identifier: http://orcid.org/0000-0001-9029-6885
authors.initials: F
authors.lastname: Campbell
mesh_terms: D002942:Circular Dichroism; D008081:Liposomes; D008561:Membrane Fusion; D008567:Membranes, Artificial; D011092:Polyethylene Glycols / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D008081:Liposomes; D008567:Membranes, Artificial; D011092:Polyethylene Glycols
keywords: PEGylation; coiled-coil peptides; liposomes; membrane fusion; photoactivation