title: Cardiac Usage of Reducible Poly(oligo-D-arginine) As a Gene Carrier for Vascular Endothelial Growth Factor Expression.
abstract: Developments of non-viral carriers have headed toward reducing cytotoxicity, which results from the use of conventional gene carriers, and enhancing gene delivery efficiency. Cys-(d-R9)-Cys repeated reducible poly(oligo-D-arginine) (rPOA) is one of the most efficient non-viral carriers for gene therapy; however, while its efficiency has been verified in the lung and brain, it is necessary to confirm its activity in each organ or tissue since there are differences of gene carrier susceptibility to among tissue types. We therefore tested the compatibility of rPOA in cardiac tissue by in vitro or in vivo experiments and confirmed its high transfection efficiency and low cytotoxicity. Moreover, substantial regenerative effects were observed following transfection with rPOA/pVEGF expression vector complexes (79% decreased infarct size) compared to polyethyleneimine (PEI) (34% decreased infarct size) in a rat myocardial infarction (MI) model. These findings suggest that rPOA efficiently enables DNA transfection in cardiac tissue and can be used as a useful non-viral therapeutic gene carrier for gene therapy in ischemic heart disease.
authors.affiliation: Severance Integrative Research Institute for Cerebral & Cardiovascular Disease, Yonsei University Health System, Seoul, South Korea.
authors.forename: Jongsu
authors.initials: J
authors.lastname: Woo
authors.affiliation: Severance Biomedical Science Institute, Yonsei University College of Medicine, Seoul, South Korea.
authors.forename: Seong-Ho
authors.initials: SH
authors.lastname: Bae
authors.affiliation: Severance Integrative Research Institute for Cerebral & Cardiovascular Disease, Yonsei University Health System, Seoul, South Korea.
authors.forename: Bokyoung
authors.initials: B
authors.lastname: Kim
authors.affiliation: Severance Integrative Research Institute for Cerebral & Cardiovascular Disease, Yonsei University Health System, Seoul, South Korea.
authors.forename: Jin Sil
authors.initials: JS
authors.lastname: Park
authors.affiliation: Severance Integrative Research Institute for Cerebral & Cardiovascular Disease, Yonsei University Health System, Seoul, South Korea.
authors.forename: Subin
authors.initials: S
authors.lastname: Jung
authors.affiliation: Department of Bioengineering, College of Engineering, Hanyang University, Seoul, South Korea.
authors.forename: Minhyung
authors.initials: M
authors.lastname: Lee
authors.affiliation: Department of Bioengineering, College of Engineering, Hanyang University, Seoul, South Korea.
authors.forename: Yong-Hee
authors.initials: YH
authors.lastname: Kim
authors.affiliation: Severance Integrative Research Institute for Cerebral & Cardiovascular Disease, Yonsei University Health System, Seoul, South Korea.|Cardiovascular Research Institute, Yonsei University College of Medicine, Seoul, South Korea.
authors.forename: Donghoon
authors.initials: D
authors.lastname: Choi
mesh_terms: D000818:Animals; D004247:DNA; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods; D006801:Humans; D009203:Myocardial Infarction / Q000235:genetics; D017202:Myocardial Ischemia / Q000235:genetics / Q000628:therapy; D010455:Peptides; D051381:Rats; D014162:Transfection; D042461:Vascular Endothelial Growth Factor A
chemical_list: D010455:Peptides; D042461:Vascular Endothelial Growth Factor A; C015462:polyarginine; D004247:DNA