title: [Rab23 enhances invasion of Sa3 cutaneous squamous cell carcinoma cells via up-regulating the expression of Rac1].
abstract: OBJECTIVE
  To investigate the effect of Ras-related protein 23 (Rab23) on the invasion capacity of Sa3 cutaneous squamous cell carcinaoma (cSCC) cells and determine whether Rab23 can enhance the invasion of Sa3 cells through regulation of Ras-related C3 botulinum toxin substrate 1 (Rac1).
  
  
  METHODS
  Sa3 cells stably expressing exogenous Rab23 or Sa3 cells deprived of endogenous Rab23 were generated using lentivirus transfection. Transwell(TM) invasion assay was used to evaluate the invasion capacity in the above-mentioned cells. The effects of Rab23 overexpression or knockdown on Rac1 expression were assessed using Western blotting. In the last experimental setting, the engineered cells were pretreated with Rac1 inhibitor Z62954982 before being subjected to the assessment of invasion capacity by Transwell(TM) assay.
  
  
  RESULTS
  Rab23 overexpression enhanced the invasion ability, whereas ablation of Rab23 attenuated the invasion in Sa3 cells. Rab23 could up-regulate the expression level of Rac1. Finally, using Rac1 inhibitor, we successfully suppressed the invasion capacity of Sa3 cells stably overexpressing Rab23.
  
  
  CONCLUSIONS
  Rab23 may enhance cSCC cell invasion via up-regulating Rac1 signaling.
authors.affiliation: Xijing Dermatology Hospital, Fourth Military Medical University, Xi' an 710032, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Miao
authors.affiliation: Xijing Dermatology Hospital, Fourth Military Medical University, Xi' an 710032, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Jian
authors.affiliation: Xijing Dermatology Hospital, Fourth Military Medical University, Xi' an 710032, China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Xijing Dermatology Hospital, Fourth Military Medical University, Xi' an 710032, China. *Corresponding author, E-mail: chengxinderm@163.com.
authors.forename: Chengxin
authors.initials: C
authors.lastname: Li
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D045744:Cell Line, Tumor; D002465:Cell Movement; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D009062:Mouth Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D009361:Neoplasm Invasiveness; D015854:Up-Regulation; D020691:rab GTP-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D020830:rac1 GTP-Binding Protein / Q000235:genetics* / Q000378:metabolism
chemical_list: C107953:RAC1 protein, human; C486614:RAB23 protein, human; D020691:rab GTP-Binding Proteins; D020830:rac1 GTP-Binding Protein