title: Trifolium pratense isoflavones improve pulmonary vascular remodelling in broiler chickens.
abstract: Pulmonary arterial remodelling is a pathological characteristic of pulmonary arterial hypertension (PAH), which contributes to the development of sustained pulmonary hypertension. The aim of this study was to investigate the effects of dietary Trifolium pratense isoflavones on pulmonary vascular remodelling in experimental broiler pulmonary hypertension syndrome. Exposure to sub-thermoneutral environmental temperatures increased broiler's pulmonary hypertension syndrome incidence and raised expression levels of nitric oxide, endothelin and endothelial nitric oxide synthase. Dietary supplementation (20 mg/kg basal diet) with Trifolium pratense isoflavones reduced pulmonary hypertension syndrome incidence and improved pulmonary vascular remodelling without affecting growth performance. The beneficial effect likely came from isoflavone improved pulmonary vascular remodelling. Isoflavone induced inducible nitric oxide synthase expression, which led to increased nitric oxide level. The nitric oxide could mediate vasorelaxation in the lungs. At the same time, the expression of endothelin was downregulated by isoflavone. Dietary supplementation of Trifolium pratense isoflavone might be a potential therapeutic strategy for the treatment of pulmonary hypertension.
authors.affiliation: College of Animal Husbandry and Veterinary Science, Henan Agricultural University, Zhengzhou, China.
authors.forename: Y B
authors.initials: YB
authors.lastname: Jiang
authors.affiliation: College of Animal Husbandry and Veterinary Science, Henan Agricultural University, Zhengzhou, China.
authors.forename: Y R
authors.initials: YR
authors.lastname: Yang
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D002645:Chickens; D004032:Diet / Q000662:veterinary; D019332:Endothelin-1 / Q000378:metabolism; D005260:Female; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D007529:Isoflavones / Q000737:chemistry / Q000494:pharmacology*; D008168:Lung / Q000098:blood supply*; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000378:metabolism; D029921:Trifolium / Q000737:chemistry*; D066253:Vascular Remodeling / Q000187:drug effects*
chemical_list: D019332:Endothelin-1; D007529:Isoflavones; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase
keywords: Trifolium pratense isoflavones; broiler; nitric oxide; pulmonary vascular remodelling