title: Optical properties of tumor tissues grown on the chorioallantoic membrane of chicken eggs: tumor model to assay of tumor response to photodynamic therapy.
abstract: Herein, the optical adequacy of a tumor model prepared with tumor cells grown on the chorioallantoic membrane (CAM) of a chicken egg is evaluated as an alternative to the mouse tumor model to assess the optimal irradiation conditions in photodynamic therapy (PDT). The optical properties of CAM and mouse tumor tissues were measured with a double integrating sphere and the inverse Monte Carlo technique in the 350- to 1000-nm wavelength range. The hemoglobin and water absorption bands observed in the CAM tumor tissue (10 eggs and 10 tumors) are equal to that of the mouse tumor tissue (8 animals and 8 tumors). The optical intersubject variability of the CAM tumor tissues meets or exceeds that of the mouse tumor tissues, and the reduced scattering coefficient spectra of CAM tumor tissues can be equated with those of mouse tumor tissues. These results confirm that the CAM tumor model is a viable alternative to the mouse tumor model, especially for deriving optimal irradiation conditions in PDT.
authors.affiliation: Osaka University, Institute for Academic Initiatives, 1-1 Yamadaoka, Suita, Osaka 565-0871, JapanbOsaka University, Graduate School of Engineering, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Norihiro
authors.initials: N
authors.lastname: Honda
authors.affiliation: Osaka University, Graduate School of Engineering, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Kariyama
authors.affiliation: Osaka University, Graduate School of Engineering, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Hisanao
authors.initials: H
authors.lastname: Hazama
authors.affiliation: SBI Pharmaceuticals Co., Ltd., Izumi Garden Tower 20F, 1-6-1, Roppongi, Minato-ku, Tokyo 106-6020, Japan.
authors.forename: Takuya
authors.initials: T
authors.lastname: Ishii
authors.affiliation: SBI Pharmaceuticals Co., Ltd., Izumi Garden Tower 20F, 1-6-1, Roppongi, Minato-ku, Tokyo 106-6020, Japan.
authors.forename: Yuya
authors.initials: Y
authors.lastname: Kitajima
authors.affiliation: SBI Pharmaceuticals Co., Ltd., Izumi Garden Tower 20F, 1-6-1, Roppongi, Minato-ku, Tokyo 106-6020, Japan.
authors.forename: Katsushi
authors.initials: K
authors.lastname: Inoue
authors.affiliation: SBI Pharmaceuticals Co., Ltd., Izumi Garden Tower 20F, 1-6-1, Roppongi, Minato-ku, Tokyo 106-6020, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Ishizuka
authors.affiliation: SBI Pharmaceuticals Co., Ltd., Izumi Garden Tower 20F, 1-6-1, Roppongi, Minato-ku, Tokyo 106-6020, Japan.
authors.forename: Tohru
authors.initials: T
authors.lastname: Tanaka
authors.affiliation: Osaka University, Graduate School of Engineering, 2-1 Yamadaoka, Suita, Osaka 565-0871, JapandOsaka University, Graduate School of Frontier Biosciences, 1-3 Yamadaoka, Suita, Osaka 565-0871, JapaneOsaka University, The Global Center for Medical Engineerin.
authors.forename: Kunio
authors.initials: K
authors.lastname: Awazu
mesh_terms: D000818:Animals; D016880:Anisotropy; D045744:Cell Line, Tumor; D002642:Chick Embryo; D049033:Chorioallantoic Membrane / Q000473:pathology*; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006454:Hemoglobins / Q000737:chemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D009010:Monte Carlo Method; D009368:Neoplasm Transplantation; D009369:Neoplasms / Q000473:pathology* / Q000628:therapy*; D055095:Optics and Photonics; D010778:Photochemotherapy / Q000379:methods*; D011524:Protoporphyrins / Q000737:chemistry; D015203:Reproducibility of Results; D012542:Scattering, Radiation; D014867:Water / Q000737:chemistry
chemical_list: D006454:Hemoglobins; D011524:Protoporphyrins; D014867:Water; C028025:protoporphyrin IX