title: Peptide Arrays for Kinome Analysis of Livestock Species.
abstract: Reversible protein phosphorylation is a central mechanism for both the transfer of intracellular information and the initiation of cellular responses. Within human medicine, considerable emphasis is placed on understanding and controlling the enzymes (kinases) that are responsible for catalyzing these modifications. This is evident in the prominent use of kinase inhibitors as drugs as well as the trend to understand complex biology and identify biomarkers via characterizations of global kinase (kinome) activity. Despite the demonstrated value of focusing on kinome activity, the application of this perspective to livestock has been restricted by the absence of appropriate research tools. In this review, we discuss the development of software platforms that facilitate the development and application of species-specific peptide arrays for kinome analysis of livestock. Examples of the application of kinomic approaches to a number of priority species (cattle, pigs, and chickens) in a number of biological contexts (infections, biomarker discovery, and food quality) are presented as are emerging trends for kinome analysis of livestock.
authors.affiliation: VIDO-InterVac, University of Saskatchewan , Saskatoon, SK , Canada ; Department of Biochemistry, University of Saskatchewan , Saskatoon, SK , Canada.
authors.forename: Joanna
authors.initials: J
authors.lastname: Daigle
authors.affiliation: VIDO-InterVac, University of Saskatchewan , Saskatoon, SK , Canada ; Department of Biochemistry, University of Saskatchewan , Saskatoon, SK , Canada.
authors.forename: Brenden
authors.initials: B
authors.lastname: Van Wyk
authors.affiliation: Department of Computer Science, University of Saskatchewan , Saskatoon, SK , Canada.
authors.forename: Brett
authors.initials: B
authors.lastname: Trost
authors.affiliation: VIDO-InterVac, University of Saskatchewan , Saskatoon, SK , Canada.
authors.forename: Erin
authors.initials: E
authors.lastname: Scruten
authors.affiliation: United States Department of Agriculture, Agricultural Research Service, SPARC , College Station, TX , USA.
authors.forename: Ryan
authors.initials: R
authors.lastname: Arsenault
authors.affiliation: Department of Computer Science, University of Saskatchewan , Saskatoon, SK , Canada.
authors.forename: Anthony
authors.initials: A
authors.lastname: Kusalik
authors.affiliation: VIDO-InterVac, University of Saskatchewan , Saskatoon, SK , Canada ; School of Public Health, University of Saskatchewan , Saskatoon, SK , Canada.
authors.forename: Philip John
authors.initials: PJ
authors.lastname: Griebel
authors.affiliation: VIDO-InterVac, University of Saskatchewan , Saskatoon, SK , Canada ; Department of Biochemistry, University of Saskatchewan , Saskatoon, SK , Canada.
authors.forename: Scott
authors.initials: S
authors.lastname: Napper
keywords: infectious disease; kinases; kinome; livestock; peptide array