title: Efficient Isolation Protocol for B and T Lymphocytes from Human Palatine Tonsils.
abstract: Tonsils form a part of the immune system providing the first line of defense against inhaled pathogens. Usually the term "tonsils" refers to the palatine tonsils situated at the lateral walls of the oral part of the pharynx. Surgically removed palatine tonsils provide a convenient accessible source of B and T lymphocytes to study the interplay between foreign pathogens and the host immune system. This video protocol describes the dissection and processing of surgically removed human palatine tonsils, followed by the isolation of the individual B and T cell populations from the same tissue sample. We present a method, which efficiently separates tonsillar B and T lymphocytes using an antibody-dependent affinity protocol. Further, we use the method to demonstrate that human adenovirus infects specifically the tonsillar T cell fraction. The established protocol is generally applicable to efficiently and rapidly isolate tonsillar B and T cell populations to study the role of different types of pathogens in tonsillar immune responses.
authors.affiliation: Department of Medical Biochemistry and Microbiology, Uppsala Biomedical Center, Uppsala University.
authors.forename: Farzaneh
authors.initials: F
authors.lastname: Assadian
authors.affiliation: Department of Surgical Sciences, Otolaryngology and Head & Neck Surgery, Akademiska sjukhuset.
authors.forename: Karl
authors.initials: K
authors.lastname: Sandström
authors.affiliation: Department of Surgical Sciences, Otolaryngology and Head & Neck Surgery, Akademiska sjukhuset.
authors.forename: Göran
authors.initials: G
authors.lastname: Laurell
authors.affiliation: Department of Medical Biochemistry and Microbiology, Uppsala Biomedical Center, Uppsala University.
authors.forename: Catharina
authors.initials: C
authors.lastname: Svensson
authors.affiliation: Department of Medical Biochemistry and Microbiology, Uppsala Biomedical Center, Uppsala University.
authors.forename: Göran
authors.initials: G
authors.lastname: Akusjärvi
authors.affiliation: Department of Medical Biochemistry and Microbiology, Uppsala Biomedical Center, Uppsala University; Tanel.Punga@imbim.uu.se.
authors.forename: Tanel
authors.initials: T
authors.lastname: Punga