title: Association of Genetic Polymorphism in the Interleukin-8 Gene with Risk of Oral Cancer and Its Correlation with Pain.
abstract: Oral cancer is a multifactorial disease process and involves complex interactions between gene to gene and gene to environmental factors. Interleukin 8 (IL-8), a pro-inflammatory cytokine, having angiogenic activity with elevated expression in tumor cells, is reported to play an essential role in oral cancer development. This study was conducted with the aim to investigate the role of IL-8 (-A251T) gene polymorphism in susceptibility, progression, and self-reporting pain in oral cancer. The single nucleotide polymorphisms of the IL-8 (-A251T) gene were screened in 300 patients with oral cancer and 300 healthy controls, by polymerase chain reaction-restriction fragment length polymorphism. Genotype and allele frequencies were evaluated by chi-square test and odds ratio (OR) with 95% confidence intervals (CIs) were used to evaluate the strength of associations. The results of the study demonstrated that IL-8 (-A251T) gene polymorphism was significantly associated with susceptibility of oral cancer, whereas its correlation with clinico-pathological status or pain due to oral cancer could not be established. The AT heterozygous (OR 5.31; CI 3.38-8.34; p 0.0001) and AA homozygous (OR 2.89; CI 1.76-4.75; p 0.0001) had a greater risk for oral cancer compared to TT homozygous. Furthermore, significantly increased values of A allele frequencies compared to T allele were observed in all patients (OR 1.56; CI 1.24-1.96; p 0.0002). Tobacco chewing and smoking were also found to influence the development of oral cancer and increased the incidence of pain in oral cancer patients. The findings of this study suggest that the IL-8 (-A251T) gene polymorphism may be associated with increased risk of oral cancer.
authors.affiliation: Department of Anaesthesiology, King George's Medical University, Lucknow, UP, India.
authors.forename: Prithvi Kumar
authors.initials: PK
authors.lastname: Singh
authors.affiliation: Department of Anaesthesiology, King George's Medical University, Lucknow, UP, India.
authors.forename: Girish
authors.initials: G
authors.lastname: Chandra
authors.affiliation: Department of Anaesthesiology, King George's Medical University, Lucknow, UP, India.
authors.forename: Jaishri
authors.initials: J
authors.lastname: Bogra
authors.affiliation: Department of Anaesthesiology, King George's Medical University, Lucknow, UP, India.
authors.forename: Rajni
authors.initials: R
authors.lastname: Gupta
authors.affiliation: Department of Surgical Oncology, King George's Medical University, Lucknow, UP, India.
authors.forename: Vijay
authors.initials: V
authors.lastname: Kumar
authors.affiliation: Department of Biochemistry, King George's Medical University, Lucknow, UP, 226003, India.
authors.forename: Syed Rizwan
authors.initials: SR
authors.lastname: Hussain
authors.affiliation: Department of Microbiology, King George's Medical University, Lucknow, UP, India.
authors.forename: Amita
authors.initials: A
authors.lastname: Jain
authors.affiliation: Department of Biochemistry, King George's Medical University, Lucknow, UP, 226003, India.
authors.forename: Abbas Ali
authors.initials: AA
authors.lastname: Mahdi
authors.affiliation: Department of Biochemistry, King George's Medical University, Lucknow, UP, 226003, India. kaleembaksh@gmail.com.
authors.forename: Mohammad Kaleem
authors.initials: MK
authors.lastname: Ahmad
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D016209:Interleukin-8 / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000150:complications* / Q000235:genetics; D010146:Pain / Q000209:etiology*; D020641:Polymorphism, Single Nucleotide
chemical_list: D016209:Interleukin-8
keywords: Angiogenesis; Cytokines; Genotype; Interleukin-8; Pain