title: Hair-bundle proteomes of avian and mammalian inner-ear utricles.
abstract: Examination of multiple proteomics datasets within or between species increases the reliability of protein identification. We report here proteomes of inner-ear hair bundles from three species (chick, mouse, and rat), which were collected on LTQ or LTQ Velos ion-trap mass spectrometers; the constituent proteins were quantified using MS2 intensities, which are the summed intensities of all peptide fragmentation spectra matched to a protein. The data are available via ProteomeXchange with identifiers PXD002410 (chick LTQ), PXD002414 (chick Velos), PXD002415 (mouse Velos), and PXD002416 (rat LTQ). The two chick bundle datasets compared favourably to a third, already-described chick bundle dataset, which was quantified using MS1 peak intensities, the summed intensities of peptides identified by high-resolution mass spectrometry (PXD000104; updated analysis in PXD002445). The mouse bundle dataset described here was comparable to a different mouse bundle dataset quantified using MS1 intensities (PXD002167). These six datasets will be useful for identifying the core proteome of vestibular hair bundles.
authors.affiliation: Department of Biochemistry and Molecular Biology, Oregon Health and Science University, Portland, OR, USA.
authors.forename: Phillip A
authors.initials: PA
authors.lastname: Wilmarth
authors.affiliation: Oregon Hearing Research Center &Vollum Institute, Oregon Health &Science University, Portland, OR, USA.
authors.forename: Jocelyn F
authors.initials: JF
authors.lastname: Krey
authors.affiliation: Department of Neuroscience, University of Virginia, Charlottesville, VA, USA.
authors.forename: Jung-Bum
authors.initials: JB
authors.lastname: Shin
authors.affiliation: Department of Public Health &Preventive Medicine, Oregon Health &Science University, Portland, OR, USA.
authors.forename: Dongseok
authors.initials: D
authors.lastname: Choi
authors.affiliation: Department of Biochemistry and Molecular Biology, Oregon Health and Science University, Portland, OR, USA.
authors.forename: Larry L
authors.initials: LL
authors.lastname: David
authors.affiliation: Department of Biochemistry and Molecular Biology, Oregon Health and Science University, Portland, OR, USA.|Oregon Hearing Research Center &Vollum Institute, Oregon Health &Science University, Portland, OR, USA.
authors.forename: Peter G
authors.identifier: http://orcid.org/0000-0002-9787-5860
authors.initials: PG
authors.lastname: Barr-Gillespie
mesh_terms: D000818:Animals; D002645:Chickens; D018069:Hair Cells, Vestibular / Q000378:metabolism; D051379:Mice; D020543:Proteome; D040901:Proteomics; D051381:Rats; D012444:Saccule and Utricle / Q000378:metabolism; D013045:Species Specificity
chemical_list: D020543:Proteome