title: Modified in situ antimicrobial susceptibility testing method based on cyanobacteria chlorophyll a fluorescence.
abstract: The chlorophyll a fluorescence based antimicrobial susceptibility testing (AST) method presented in a previous work was based on the measurement of Chl a fluorescence of the gram(-) cyanobacterium Synechococcus sp. PCC 7942. Synechococcus sp. PCC 7942 as a gram(-) bacterium is affected by antibacterial agents via mechanisms affecting all gram(-) bacteria, however, as an exclusively phototrophic organism it would also be affected by photosynthesis inhibitory action of an agent that otherwise has no antibacterial properties. In this report, the method is modified by replacing the exclusively phototrophic Synechococcus sp. PCC 7942 with the Synechocystis sp. PCC 6714, capable of both phototrophic and heterotrophic growth in order to add versatility and better reflect the antibacterial effects of surfaces under study towards nonphotosynthetic bacteria.
authors.affiliation: Institute of Nanoscience and Nanotechnology, N.C.S.R. Demokritos Terma Patriarchou Grigoriou & Neapoleos, 15310 Ag.Paraskevi Attikis, Athens, Greece.
authors.forename: Nikolaos S
authors.initials: NS
authors.lastname: Heliopoulos
authors.affiliation: Institute of Biosciences and Applications, N.C.S.R. Demokritos, Terma Patriarchou Grigoriou & Neapoleos, 15310 Ag. Paraskevi Attikis, Athens, Greece.
authors.forename: Angeliki
authors.initials: A
authors.lastname: Galeou
authors.affiliation: Institute of Nanoscience and Nanotechnology, N.C.S.R. Demokritos Terma Patriarchou Grigoriou & Neapoleos, 15310 Ag.Paraskevi Attikis, Athens, Greece. Electronic address: s.papageorgiou@inn.demokritos.gr.
authors.forename: Sergios K
authors.initials: SK
authors.lastname: Papageorgiou
authors.affiliation: Institute of Nanoscience and Nanotechnology, N.C.S.R. Demokritos Terma Patriarchou Grigoriou & Neapoleos, 15310 Ag.Paraskevi Attikis, Athens, Greece.
authors.forename: Evangelos P
authors.initials: EP
authors.lastname: Favvas
authors.affiliation: Institute of Nanoscience and Nanotechnology, N.C.S.R. Demokritos Terma Patriarchou Grigoriou & Neapoleos, 15310 Ag.Paraskevi Attikis, Athens, Greece.
authors.forename: Fotios K
authors.initials: FK
authors.lastname: Katsaros
authors.affiliation: Institute of Biosciences and Applications, N.C.S.R. Demokritos, Terma Patriarchou Grigoriou & Neapoleos, 15310 Ag. Paraskevi Attikis, Athens, Greece.
authors.forename: Kostas
authors.initials: K
authors.lastname: Stamatakis
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology*; D002734:Chlorophyll / Q000032:analysis / Q000737:chemistry*; D000077194:Chlorophyll A; D004783:Environmental Microbiology; D005453:Fluorescence; D008826:Microbial Sensitivity Tests / Q000379:methods*; D010788:Photosynthesis / Q000187:drug effects; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013499:Surface Properties; D046940:Synechococcus / Q000737:chemistry* / Q000187:drug effects* / Q000254:growth & development; D046939:Synechocystis / Q000737:chemistry / Q000187:drug effects / Q000254:growth & development; D013784:Textiles / Q000382:microbiology
chemical_list: D000890:Anti-Infective Agents; D002734:Chlorophyll; D000077194:Chlorophyll A
keywords: Antibacterial properties; Chlorophyll fluorescence; Cyanobacteria spp.; Textiles