title: Cetirizine Quantification by High-Performance Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS).
abstract: A multiple reaction monitoring (MRM), positive ion electrospray ionization, LC/MS/MS method is described for the quantification of cetirizine. The compound was isolated from human plasma by protein precipitation using acetonitrile. Cetirizine d4 was used as an internal standard. Chromatographic conditions were achieved using a C18 column and a combination of ammonium acetate, water, and methanol as the mobile phase. MRMs were: cetirizine, 389.26 → 165.16, 201.09; cetirizine d4, 393.09 → 165.15, 201.10. Calibration curves were constructed by plotting the peak area ratios of the calibrators' target MRM transition area to labeled internal standard target MRM transition area versus concentration.
authors.affiliation: Department of Pathology and Laboratory Medicine, Children's Mercy Hospitals and Clinics, University of Missouri School of Medicine, 2401 Gillham Road, Kansas City, MO, 64108, USA.
authors.forename: Ada
authors.initials: A
authors.lastname: Munar
authors.affiliation: Department of Pathology and Laboratory Medicine, Children's Mercy Hospitals and Clinics, 2401 Gillham Road, Kansas City, MO, 64108, USA.
authors.forename: Clint
authors.initials: C
authors.lastname: Frazee
authors.affiliation: Department of Pediatrics, Children's Mercy Hospitals and Clinics, Kansas City, MO, USA.
authors.forename: Bridgette
authors.initials: B
authors.lastname: Jones
authors.affiliation: Department of Pathology and Laboratory Medicine, Children's Mercy Hospitals and Clinics, 2401 Gillham Road, Kansas City, MO, 64108, USA. ugarg@cmh.edu.
authors.forename: Uttam
authors.initials: U
authors.lastname: Garg
mesh_terms: D018926:Anti-Allergic Agents / Q000097:blood*; D017332:Cetirizine / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D016903:Drug Monitoring / Q000379:methods; D039563:Histamine H1 Antagonists, Non-Sedating / Q000097:blood*; D006801:Humans; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D018926:Anti-Allergic Agents; D039563:Histamine H1 Antagonists, Non-Sedating; D017332:Cetirizine
keywords: Allergic rhinitis and chronic urticaria; Antihistamine; Cetirizine; H1-receptor