title: TRP channel mediated neuronal activation and ablation in freely behaving zebrafish.
abstract: The zebrafish (Danio rerio) is a useful vertebrate model system in which to study neural circuits and behavior, but tools to modulate neurons in freely behaving animals are limited. As poikilotherms that live in water, zebrafish are amenable to thermal and pharmacological perturbations. We exploit these properties by using transient receptor potential (TRP) channels to activate or ablate specific neuronal populations using the chemical and thermal agonists of heterologously expressed TRPV1, TRPM8 and TRPA1.
authors.affiliation: Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, California, USA.
authors.forename: Shijia
authors.initials: S
authors.lastname: Chen
authors.affiliation: Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, California, USA.
authors.forename: Cindy N
authors.initials: CN
authors.lastname: Chiu
authors.affiliation: Department of Neurobiology and Behavior, Cornell University, Ithaca, New York, USA.
authors.forename: Kimberly L
authors.initials: KL
authors.lastname: McArthur
authors.affiliation: Department of Neurobiology and Behavior, Cornell University, Ithaca, New York, USA.
authors.forename: Joseph R
authors.initials: JR
authors.lastname: Fetcho
authors.affiliation: Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, California, USA.
authors.forename: David A
authors.initials: DA
authors.lastname: Prober
mesh_terms: D000818:Animals; D004625:Embryo, Nonmammalian; D005786:Gene Expression Regulation / Q000502:physiology*; D011984:Sensory Receptor Cells / Q000502:physiology*; D050051:Transient Receptor Potential Channels / Q000502:physiology*; D015027:Zebrafish
chemical_list: D050051:Transient Receptor Potential Channels