title: Myostatin inhibits porcine intramuscular preadipocyte differentiation in vitro.
abstract: This study assessed the effect of myostatin on adipogenesis by porcine intramuscular preadipocytes. Intramuscular preadipocytes were isolated from the longissimus dorsi muscle of newborn pigs. Myostatin inhibited intramuscular preadipocyte differentiation in a dose-dependent manner. Myostatin treatment during preadipocyte differentiation significantly (P < 0.05) inhibited the expression of the adipogenic marker genes CCAAT/enhancer-binding protein β, CCAAT/enhancer-binding protein α, peroxisome proliferator-activated receptor γ, sterol regulatory element-binding protein-1c, fatty acid-binding protein, and adiponectin. Myostatin also significantly (P < 0.05) reduced the release of glycerol and decreased both adipose triglyceride lipase and hormone-sensitive lipase expression in intramuscular adipocytes. Our study suggests that myostatin acts as an extrinsic regulatory factor in regulating intramuscular adipogenesis.
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China; College of Public Health, Nantong University, Nantong, 226019, China.
authors.forename: W X
authors.initials: WX
authors.lastname: Sun
authors.affiliation: Department of Animal Sciences, Washington State University, Pullman, WA, 99164-6351, USA.
authors.forename: M V
authors.initials: MV
authors.lastname: Dodson
authors.affiliation: Department of Animal Sciences, Washington State University, Pullman, WA, 99164-6351, USA.
authors.forename: Z H
authors.initials: ZH
authors.lastname: Jiang
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: S G
authors.initials: SG
authors.lastname: Yu
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: W W
authors.initials: WW
authors.lastname: Chu
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China. Electronic address: jiechen@njau.edu.cn.
authors.forename: J
authors.initials: J
authors.lastname: Chen
mesh_terms: D017667:Adipocytes / Q000737:chemistry / Q000502:physiology*; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects* / Q000502:physiology; D002478:Cells, Cultured; D003470:Culture Media; D005786:Gene Expression Regulation; D018482:Muscle, Skeletal / Q000502:physiology*; D055435:Myostatin / Q000494:pharmacology*; D013552:Swine; D046509:Tissue Culture Techniques
chemical_list: D003470:Culture Media; D055435:Myostatin
keywords: Differentiation; Intramuscular preadipocytes; Lipolysis; Myostatin