title: Simultaneous determination of rosuvastatin and amlodipine in human plasma using tandem mass spectrometry: Application to disposition kinetics.
abstract: The liquid chromatography-tandem mass spectrometric assay method for the simultaneous determination of rosuvastatin and amlodipine in human plasma using deuterated analogs as internal standards has been developed and validated. The analytes were extracted from 100 μL aliquots of human plasma via liquid-liquid extraction using a mixture of ethyl acetate and n-hexane (80:20, v/v) as an extraction solvent. The optimized mobile phase was composed of 0.1% formic acid in 5 mM ammonium acetate, methanol, and acetonitrile (20:20:60, v/v/v) and delivered at a flow rate of 0.75 mL/min. The calibration curve obtained was linear (R (2) ⩾ 0.999) over the concentration range of 0.52-51.77 ng/mL for rosuvastatin and 0.10-10.07 ng/mL for amlodipine. A sample turnover rate of less than 2.5 min makes it an attractive procedure in high-throughput bioanalysis of rosuvastatin and amlodipine. The present method was found to be applicable to clinical studies and the results were authenticated by incurred sample reanalysis.
authors.affiliation: Department of Pharmaceutical Sciences, Jawaharlal Nehru Technological University Kakinada, Kakinada 533 003, India ; Department of Pharmaceutical Analysis and Pharmacology, Geethanjali College of Pharmacy, Cheeryal, Hyderabad 501 301, India.
authors.forename: Anjaneyulu
authors.initials: A
authors.lastname: Narapusetti
authors.affiliation: Department of Chemistry, Yogi Vemana University, Kadapa 516 003, India.
authors.forename: Syama Sundar
authors.initials: SS
authors.lastname: Bethanabhatla
authors.affiliation: Department of Pharmaceutical Chemistry, Surya School of Pharmacy, NH-45, GST Road, Vikravandi, Villpuram 605 652, India.
authors.forename: Anbazhagan
authors.initials: A
authors.lastname: Sockalingam
authors.affiliation: Department of Pharmaceutical Analysis and Pharmacology, Geethanjali College of Pharmacy, Cheeryal, Hyderabad 501 301, India.
authors.forename: Nagakishore
authors.initials: N
authors.lastname: Repaka
authors.affiliation: Department of Pharmacy, Palamuru University, Mahabubnagar 509 001, India.
authors.forename: Veldandi
authors.initials: V
authors.lastname: Saritha
keywords: Amlodipine; Human plasma; LC/MS/MS; Method validation; Pharmacokinetics; Rosuvastatin