title: Combinatorial Biomolecular Nanopatterning for High-Throughput Screening of Stem-Cell Behavior.
abstract: A novel combinatorial biomolecular nanopatterning method is reported, in which multiple biomolecular ligands can be patterned in multiple nanoscale dimensions on a single surface. The applicability of the combinatorial platform toward cell-biology applications is demonstrated by screening the adhesion behavior of a population of human dental pulp stem cell (hDPSC) on 64 combinations of nanopatterned extracellular matrix (ECM) proteins in parallel.
authors.affiliation: Interdisciplinary Nanoscience Center (iNANO), Faculty of Science and Technology, Aarhus University, 8000, Aarhus C, Denmark.
authors.forename: Yacoub Y I
authors.initials: YY
authors.lastname: Amin
authors.affiliation: Interdisciplinary Nanoscience Center (iNANO), Faculty of Science and Technology, Aarhus University, 8000, Aarhus C, Denmark.
authors.forename: Kasper
authors.initials: K
authors.lastname: Runager
authors.affiliation: Laboratory of Biophysics and Surface Analysis, School of Pharmacy, University of Nottingham, NG7 2RD, Nottingham, UK.
authors.forename: Fabio
authors.initials: F
authors.lastname: Simoes
authors.affiliation: Laboratory of Biophysics and Surface Analysis, School of Pharmacy, University of Nottingham, NG7 2RD, Nottingham, UK.
authors.forename: Adam
authors.initials: A
authors.lastname: Celiz
authors.affiliation: Laboratory of Biophysics and Surface Analysis, School of Pharmacy, University of Nottingham, NG7 2RD, Nottingham, UK.
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Taresco
authors.affiliation: Department of Chemical Sciences, University of Padova, 35122, Padova, Italy.
authors.forename: Roberto
authors.initials: R
authors.lastname: Rossi
authors.affiliation: Interdisciplinary Nanoscience Center (iNANO), Faculty of Science and Technology, Aarhus University, 8000, Aarhus C, Denmark.
authors.forename: Jan J
authors.initials: JJ
authors.lastname: Enghild
authors.affiliation: Department of Dentistry, Faculty of Health, Aarhus University, 8000, Aarhus C, Denmark.
authors.forename: Lisbeth A
authors.initials: LA
authors.lastname: Abildtrup
authors.affiliation: Department of Dentistry, Faculty of Health, Aarhus University, 8000, Aarhus C, Denmark.
authors.forename: David C E
authors.initials: DC
authors.lastname: Kraft
authors.affiliation: Interdisciplinary Nanoscience Center (iNANO), Faculty of Science and Technology, Aarhus University, 8000, Aarhus C, Denmark.
authors.forename: Duncan S
authors.initials: DS
authors.lastname: Sutherland
authors.affiliation: Laboratory of Biophysics and Surface Analysis, School of Pharmacy, University of Nottingham, NG7 2RD, Nottingham, UK.
authors.forename: Morgan R
authors.initials: MR
authors.lastname: Alexander
authors.affiliation: Interdisciplinary Nanoscience Center (iNANO), Faculty of Science and Technology, Aarhus University, 8000, Aarhus C, Denmark.
authors.forename: Morten
authors.initials: M
authors.lastname: Foss
authors.affiliation: Interdisciplinary Nanoscience Center (iNANO), Faculty of Science and Technology, Aarhus University, 8000, Aarhus C, Denmark.
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Ogaki
mesh_terms: D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000379:methods*; D003782:Dental Pulp / Q000166:cytology; D006801:Humans; D036103:Nanotechnology / Q000379:methods*; D013234:Stem Cells / Q000166:cytology*
keywords: cell adhesion; combinatorial; high throughput; nanofabrication; protein patterning