title: Comparative Study of Phenolic Profile, Antioxidant Capacity, and Color-composition Relation of Roselle Cultivars with Contrasting Pigmentation.
abstract: Roselle is a plant that accumulates anthocyanins significantly, hence its importance as food coloring and as a source of antioxidant compounds for human health. This study was aimed to determine phenolic composition and antioxidant capacity of methanolic extracts, and beverages obtained from native roselle cultivars in Mexico (Negra, Sudan, Rosa and Blanca) with different degrees of pigmentation, and to establish the color-composition relationship. Chromatographic methods were used to determine phenolic compounds: flavanols, flavonols, benzoic, hibiscus and phenolic acids as well as two main anthocyanins (cyanidin 3-sambubioside and delphinidin 3-sambubioside). The antioxidant capacity was evaluated by ABTS and FRAP assays. Tristimulus colorimetry showed to be a useful technique to determine the color-composition relationship, leading to equations that allowed to predict anthocyanin content of roselle (R > 0.84). Also, a stepwise linear discriminant analysis (SLDA) was developed in order to classify roselle cultivars. The obtained mathematical model could be an important tool to be used in colorimetric characterization of functional compounds used in food processing.
authors.affiliation: Instituto Politécnico Nacional, CEPROBI, Carretera Yautepec-Jojutla Km. 6, Calle CEPROBI 8, Col. San Isidro, Yautepec, Morelos, Mexico, C.P. 62731.
authors.forename: Gustavo A
authors.initials: GA
authors.lastname: Camelo-Méndez
authors.affiliation: Food Colour & Quality Laboratory, Department Nutrition & Food Science, Facultad de Farmacia, Universidad de Sevilla, 41012, Sevilla, Spain.
authors.forename: M José
authors.initials: MJ
authors.lastname: Jara-Palacios
authors.affiliation: Food Colour & Quality Laboratory, Department Nutrition & Food Science, Facultad de Farmacia, Universidad de Sevilla, 41012, Sevilla, Spain.
authors.forename: M Luisa
authors.initials: ML
authors.lastname: Escudero-Gilete
authors.affiliation: Food Colour & Quality Laboratory, Department Nutrition & Food Science, Facultad de Farmacia, Universidad de Sevilla, 41012, Sevilla, Spain.
authors.forename: Belén
authors.initials: B
authors.lastname: Gordillo
authors.affiliation: Department of Analytical Chemistry, Facultad de Farmacia, Universidad de Sevilla, 41012, Sevilla, Spain.
authors.forename: Dolores
authors.initials: D
authors.lastname: Hernanz
authors.affiliation: Centro de Investigación y de Estudios Avanzados del IPN, Unidad Irapuato, Irapuato, Guanajuato, Mexico.
authors.forename: Octavio
authors.initials: O
authors.lastname: Paredes-López
authors.affiliation: Instituto Politécnico Nacional, CEPROBI, Carretera Yautepec-Jojutla Km. 6, Calle CEPROBI 8, Col. San Isidro, Yautepec, Morelos, Mexico, C.P. 62731.
authors.forename: Pablo E
authors.initials: PE
authors.lastname: Vanegas-Espinoza
authors.affiliation: Instituto Politécnico Nacional, CEPROBI, Carretera Yautepec-Jojutla Km. 6, Calle CEPROBI 8, Col. San Isidro, Yautepec, Morelos, Mexico, C.P. 62731. adelvillarm@ipn.mx.
authors.forename: Alma A
authors.initials: AA
authors.lastname: Del Villar-Martínez
authors.affiliation: Food Colour & Quality Laboratory, Department Nutrition & Food Science, Facultad de Farmacia, Universidad de Sevilla, 41012, Sevilla, Spain.
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Heredia
mesh_terms: D000872:Anthocyanins / Q000032:analysis* / Q000378:metabolism; D000975:Antioxidants / Q000032:analysis* / Q000378:metabolism; D001628:Beverages / Q000032:analysis; D003116:Color; D004187:Disaccharides / Q000032:analysis / Q000378:metabolism; D031584:Hibiscus / Q000737:chemistry*; D008800:Mexico; D015999:Multivariate Analysis; D010636:Phenols / Q000032:analysis* / Q000378:metabolism; D010858:Pigmentation; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D000872:Anthocyanins; D000975:Antioxidants; D004187:Disaccharides; D010636:Phenols; D010936:Plant Extracts; C505011:delphinidin 3-sambubioside; C546691:cyanidin 3-sambubioside
keywords: Antioxidant capacity; Color-composition relationship; Hibiscus sabdariffa; Multivariate statistical analysis; Phenolic compounds; Tristimulus colorimetry