title: Reprogramming mitochondrial metabolism in macrophages as an anti-inflammatory signal.
abstract: Mitochondria are master regulators of metabolism. Mitochondria generate ATP by oxidative phosphorylation using pyruvate (derived from glucose and glycolysis) and fatty acids (FAs), both of which are oxidized in the Krebs cycle, as fuel sources. Mitochondria are also an important source of reactive oxygen species (ROS), creating oxidative stress in various contexts, including in the response to bacterial infection. Recently, complex changes in mitochondrial metabolism have been characterized in mouse macrophages in response to varying stimuli in vitro. In LPS and IFN-γ-activated macrophages (M1 macrophages), there is decreased respiration and a broken Krebs cycle, leading to accumulation of succinate and citrate, which act as signals to alter immune function. In IL-4-activated macrophages (M2 macrophages), the Krebs cycle and oxidative phosphorylation are intact and fatty acid oxidation (FAO) is also utilized. These metabolic alterations in response to the nature of the stimulus are proving to be determinants of the effector functions of M1 and M2 macrophages. Furthermore, reprogramming of macrophages from M1 to M2 can be achieved by targeting metabolic events. Here, we describe the role that metabolism plays in macrophage function in infection and immunity, and propose that reprogramming with metabolic inhibitors might be a novel therapeutic approach for the treatment of inflammatory diseases.
authors.affiliation: Trinity Biomedical Sciences Institute, Trinity College Dublin, Ireland.
authors.forename: Evanna L
authors.initials: EL
authors.lastname: Mills
authors.affiliation: Trinity Biomedical Sciences Institute, Trinity College Dublin, Ireland.
authors.forename: Luke A
authors.initials: LA
authors.lastname: O'Neill
mesh_terms: D000818:Animals; D006801:Humans; D007249:Inflammation / Q000276:immunology*; D008264:Macrophages / Q000276:immunology* / Q000378:metabolism*; D008928:Mitochondria / Q000276:immunology* / Q000378:metabolism*
keywords: Inflammatory diseases; M1 and M2 macrophages; Metabolism; Mitochondria