title: No evidence for viral sequences in five lepidic adenocarcinomas (former "BAC") by a high-throughput sequencing approach.
abstract: BACKGROUND
  The hypothesis of an infectious etiology of the formerly named bronchiolo-alveolar carcinoma (BAC) has raised controversy. We investigated tumor lung tissues from five patients with former BAC histology using high-throughput sequencing technologies to discover potential viruses present in this type of lung cancer. Around 180 million single reads of 100 bases were generated for each BAC sample.
  
  
  RESULTS
  None of the reads showed a significant similarity for Jaagsiekte sheep retrovirus (JSRV) and no other viruses were found except for endogenous retroviruses.
  
  
  CONCLUSIONS
  In conclusion, we have demonstrated the absence of JSRV and other known human viruses in five samples of well-characterized lepidic adenocarcinoma.
authors.affiliation: Epidemiology and Physiopathology of Oncogenic Viruses Unit, Institut Pasteur, 28 rue du Docteur Roux, 75015, Paris, France. nicolas.berthet@ird.fr.|Centre National de la Recherche Scientifique, UMR 3569, 28 rue du Docteur Roux, 75015, Paris, France. nicolas.berthet@ird.fr.|Département Zoonoses et Maladies Emergentes, Groupe Syndromes Cliniques et Virus Associés, Centre International de Recherches Médicales de Franceville (CIRMF), BP 769, Franceville, Gabon. nicolas.berthet@ird.fr.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Berthet
authors.affiliation: Viruses and RNAi Unit, Institut Pasteur, 28 rue du Docteur Roux, 75015, Paris, France. lionel.frangeul@pasteur.fr.
authors.forename: Lionel
authors.initials: L
authors.lastname: Frangeul
authors.affiliation: INSERM - U981, 94805, Villejuif, France. Ken.OLAUSSEN@gustaveroussy.fr.|Gustave Roussy, DHU TORINO, 94805, Villejuif, Paris, France. Ken.OLAUSSEN@gustaveroussy.fr.|Univ Paris-Sud, UMR-S981, 94805, Villejuif, Paris, France. Ken.OLAUSSEN@gustaveroussy.fr.
authors.forename: Ken André
authors.initials: KA
authors.lastname: Olaussen
authors.affiliation: INSERM U823, Institut Albert Bonniot-Université Joseph Fourier, Grenoble Cedex 09, France. EBrambilla@chu-grenoble.fr.|Département d'Anatomie et Cytologie Pathologiques, CHU Albert Michallon, BP 217, 38043, Grenoble Cedex 09, France. EBrambilla@chu-grenoble.fr.
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Brambilla
authors.affiliation: INSERM - U981, 94805, Villejuif, France. Nicolas.DORVAULT@gustaveroussy.fr.|Gustave Roussy, DHU TORINO, 94805, Villejuif, Paris, France. Nicolas.DORVAULT@gustaveroussy.fr.|Univ Paris-Sud, UMR-S981, 94805, Villejuif, Paris, France. Nicolas.DORVAULT@gustaveroussy.fr.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Dorvault
authors.affiliation: Département thoracique, Institut Mutualiste Montsouris, 42 Boulevard Jourdan, 75014, Paris, France. philippe.girard@imm.fr.
authors.forename: Philippe
authors.initials: P
authors.lastname: Girard
authors.affiliation: Département d'anatomie pathologique, Institut Mutualiste Montsouris, 42 Boulevard Jourdan, 75014, Paris, France. Pierre.Validire@imm.fr.
authors.forename: Pierre
authors.initials: P
authors.lastname: Validire
authors.affiliation: INSERM, U999, 92350, Le Plessis-Robinson, France. e.fadel@ccml.fr.|Univ Paris-Sud, UMR-S999, 92350, Le Plessis-Robinson, France. e.fadel@ccml.fr.|Department of Thoracic and Vascular Surgery and Heart-Lung Transplantation, Centre Chirurgical Marie Lannelongue, 92350, Le Plessis-Robinson, France. e.fadel@ccml.fr.
authors.forename: Elie
authors.initials: E
authors.lastname: Fadel
authors.affiliation: Plateforme de Génomique, Institut Pasteur, 28 rue du Docteur Roux, 75015, Paris, France. christiane.bouchier@pasteur.fr.
authors.forename: Christiane
authors.initials: C
authors.lastname: Bouchier
authors.affiliation: Epidemiology and Physiopathology of Oncogenic Viruses Unit, Institut Pasteur, 28 rue du Docteur Roux, 75015, Paris, France. antoine.gessain@pasteur.fr.|Centre National de la Recherche Scientifique, UMR 3569, 28 rue du Docteur Roux, 75015, Paris, France. antoine.gessain@pasteur.fr.
authors.forename: Antoine
authors.initials: A
authors.lastname: Gessain
authors.affiliation: INSERM - U981, 94805, Villejuif, France. Jean-Charles.Soria@gustaveroussy.fr.|Gustave Roussy, DHU TORINO, 94805, Villejuif, Paris, France. Jean-Charles.Soria@gustaveroussy.fr.|Univ Paris-Sud, UMR-S981, 94805, Villejuif, Paris, France. Jean-Charles.Soria@gustaveroussy.fr.
authors.forename: Jean-Charles
authors.initials: JC
authors.lastname: Soria
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000821:virology; D002282:Adenocarcinoma, Bronchiolo-Alveolar / Q000235:genetics* / Q000821:virology; D000368:Aged; D000818:Animals; D020077:Endogenous Retroviruses / Q000235:genetics* / Q000502:physiology; D005260:Female; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006801:Humans; D022141:Jaagsiekte sheep retrovirus / Q000235:genetics* / Q000502:physiology; D008168:Lung / Q000473:pathology / Q000821:virology; D008175:Lung Neoplasms / Q000235:genetics* / Q000821:virology; D008297:Male; D008875:Middle Aged; D011648:Pulmonary Adenomatosis, Ovine / Q000235:genetics / Q000821:virology; D012756:Sheep