title: Mammalian cells exposed to ionizing radiation: Structural and biochemical aspects.
abstract: Acute or chronic exposure to ionizing radiation is a factor that may be hazardous to health. It has been reported that exposure to low doses of radiation (less than 50 mSv/year) and subsequently exposure to high doses produces greater effects in people. It has been reported that people who have been exposed to low doses of radiation (less than 50 mSv/year) and subsequently are exposed to high doses, have greater effects. However, at a molecular and biochemical level, it is an unknown alteration. This study, analyzes the susceptibility of a biological system (HeLa ATCC CCL-2 human cervix cancer cell line) to ionizing radiation (6 and 60 mSv/90 s). Our research considers multiple variables such as: total protein profile, mitochondrial metabolic activity (XTT assay), cell viability (Trypan blue exclusion assay), cytoskeleton (actin microfilaments), nuclei (DAPI), and genomic DNA. The results indicate, that cells exposed to ionizing radiation show structural alterations in nuclear phenotype and aneuploidy, further disruption in the tight junctions and consequently on the distribution of actin microfilaments. Similar alterations were observed in cells treated with a genotoxic agent (200 μM H2O2/1h). In conclusion, this multi-criteria assessment enables precise comparisons of the effects of radiation between various line cells. However, it is necessary to determine stress markers for integration of the effects of ionizing radiation.
authors.affiliation: Departamento de Biología, DCNE, Universidad de Guanajuato, Noria Alta s/n, Col. Noria Alta, 36250 Guanajuato, Guanajuato, México. Electronic address: myrna.sabanero@gmail.com.
authors.forename: Myrna
authors.initials: M
authors.lastname: Sabanero
authors.affiliation: Departamento de Ingeniería Física, DCI, Universidad de Guanajuato, Loma del Bosque 103, Lomas del Campestre, 37150 León, Guanajuato, México.
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Azorín-Vega
authors.affiliation: Departamento de Biología, DCNE, Universidad de Guanajuato, Noria Alta s/n, Col. Noria Alta, 36250 Guanajuato, Guanajuato, México.
authors.forename: Lérida Liss
authors.initials: LL
authors.lastname: Flores-Villavicencio
authors.affiliation: Departamento de Ecología, CUCBA, Universidad de Guadalajara, Las Agujas, Zapopan, Jalisco 45100, México.
authors.forename: J
authors.initials: J
authors.lastname: Pedro Castruita-Dominguez
authors.affiliation: Departamento de Ingeniería Física, DCI, Universidad de Guanajuato, Loma del Bosque 103, Lomas del Campestre, 37150 León, Guanajuato, México.
authors.forename: Miguel Angel
authors.initials: MA
authors.lastname: Vallejo
authors.affiliation: Departamento de Ciencias Médicas, DCS, Universidad de Guanajuato, 20 de Enero No. 929, Col. Obregón, C.P. 37000 León, Gto., Mexico.
authors.forename: Gloria
authors.initials: G
authors.lastname: Barbosa-Sabanero
authors.affiliation: Departamento de Ingeniería Física, DCI, Universidad de Guanajuato, Loma del Bosque 103, Lomas del Campestre, 37150 León, Guanajuato, México.
authors.forename: Teodoro
authors.initials: T
authors.lastname: Cordova-Fraga
authors.affiliation: Departamento de Ingeniería Física, DCI, Universidad de Guanajuato, Loma del Bosque 103, Lomas del Campestre, 37150 León, Guanajuato, México. Electronic address: modesto@fisica.ugto.mx.
authors.forename: Modesto
authors.initials: M
authors.lastname: Sosa-Aquino
mesh_terms: D002467:Cell Nucleus / Q000473:pathology / Q000528:radiation effects*; D048429:Cell Size / Q000528:radiation effects*; D003599:Cytoskeleton / Q000473:pathology / Q000528:radiation effects*; D004249:DNA Damage; D004307:Dose-Response Relationship, Radiation; D004734:Energy Metabolism / Q000502:physiology / Q000528:radiation effects; D006367:HeLa Cells; D006801:Humans; D008928:Mitochondria / Q000378:metabolism* / Q000528:radiation effects; D011839:Radiation, Ionizing
keywords: DNA; Ionized radiation; Organelles structural; Susceptibility cells