title: Constructing 3D microtubule networks using holographic optical trapping.
abstract: Developing abilities to assemble nanoscale structures is a major scientific and engineering challenge. We report a technique which allows precise positioning and manipulation of individual rigid filaments, enabling construction of custom-designed 3D filament networks. This approach uses holographic optical trapping (HOT) for nano-positioning and microtubules (MTs) as network building blocks. MTs are desirable engineering components due to their high aspect ratio, rigidity, and their ability to serve as substrate for directed nano-transport, reflecting their roles in the eukaryotic cytoskeleton. The 3D architecture of MT cytoskeleton is a significant component of its function, however experimental tools to study the roles of this geometric complexity in a controlled environment have been lacking. We demonstrate the broad capabilities of our system by building a self-supporting 3D MT-based nanostructure and by conducting a MT-based transport experiment on a dynamically adjustable 3D MT intersection. Our methodology not only will advance studies of cytoskeletal networks (and associated processes such as MT-based transport) but will also likely find use in engineering nanostructures and devices.
authors.affiliation: Department of Physics &Astronomy, Department of Biology, Center for Cell and Genome Science, University of Utah, 84112 Salt Lake City UT.
authors.forename: J
authors.initials: J
authors.lastname: Bergman
authors.affiliation: Department of Physics &Astronomy, Department of Biology, Center for Cell and Genome Science, University of Utah, 84112 Salt Lake City UT.
authors.forename: O
authors.initials: O
authors.lastname: Osunbayo
authors.affiliation: Department of Physics &Astronomy, Department of Biology, Center for Cell and Genome Science, University of Utah, 84112 Salt Lake City UT.
authors.forename: M
authors.initials: M
authors.lastname: Vershinin