title: Mongoliibacter rubergen. nov., sp. nov., a haloalkalitolerant bacterium of the familyCyclobacteriaceaeisolated from a haloalkaline lake.
abstract: A novel haloalkalitolerant, rod-shaped bacterium, designated strain YIM 4-4T, was isolated from the surface water of the Dugerno lake, a haloalkaline lake in Inner Mongolia. The taxonomy of strain YIM 4-4Twas investigated by a polyphasic approach. Strain YIM 4-4Twas Gram-stain-negative, strictly aerobic, non-motile and formed red colonies. Optimal growth conditions were 28 °C, pH 8.0-11.0 and 0.5-2 % NaCl. The major respiratory quinone was menaquinone-7 (MK-7). The polar lipid profile was composed predominantly of phosphatidylethanolamine, six unidentified polar lipids, one phospholipid and one aminolipid. The predominant cellular fatty acids (>5 %) were iso-C15 : 0, iso-C17 : 1I/anteiso-C17 : 1B, iso-C16 : 1G, iso-C17 : 03-OH, C16 : 1ω7c/C16 : 1ω6cand iso-C16 : 1. The genomic DNA G+C content was 43.0 mol%. 16S rRNA gene sequence analysis indicated that the members of the generaCecembia,Fontibacter,AquiflexumandIndibacterof the familyCyclobacteriaceae(phylumBacteroidetes) were the most closely related, with 16S rRNA gene sequence similarities ranging from 93.6 to 94.2 %. Other members of the familyCyclobacteriaceaeshowed sequence similarities < 93.0 %. On the basis of phenotypic, chemotaxonomic and phylogenetic properties, strain YIM 4-4Trepresents a novel species of a new genus, for which the nameMongoliibacter rubergen. nov., sp. nov. is proposed. The type strain is YIM 4-4T( = CCTCC AB 2012966T= DSM 27929T).
authors.affiliation: Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, PR China.
authors.forename: Yong-Xia
authors.initials: YX
authors.lastname: Wang
authors.affiliation: Institution for Applied and Environmental Microbiology, Inner Mongolia Agriculture University, Huhhot 010018, PR China.|Inner Mongolia Yili Industrial Group, Co., Ltd, Huhhot, Inner Mongolia 010110, PR China.
authors.forename: Ya-Peng
authors.initials: YP
authors.lastname: Liu
authors.affiliation: Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, PR China.
authors.forename: Qing-Qing
authors.initials: QQ
authors.lastname: Huo
authors.affiliation: Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, PR China.
authors.forename: Ya-Ping
authors.initials: YP
authors.lastname: Li
authors.affiliation: Institution for Applied and Environmental Microbiology, Inner Mongolia Agriculture University, Huhhot 010018, PR China.
authors.forename: Fu-Ying
authors.initials: FY
authors.lastname: Feng
authors.affiliation: Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Xiao
authors.affiliation: Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, PR China.
authors.forename: Zhang-Gui
authors.initials: ZG
authors.lastname: Ding
authors.affiliation: Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, PR China.
authors.forename: Yong-Hong
authors.initials: YH
authors.lastname: Lai
authors.affiliation: Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, PR China.
authors.forename: Xiao-Long
authors.initials: XL
authors.lastname: Cui