title: Liver epithelial cells inhibit proliferation and invasiveness of hepatoma cells.
abstract: Hepatocellular carcinoma (HCC) is a worldwide malignancy with poor prognosis. Liver progenitors or stem cells could be a potential therapy for HCC treatment since they migrate toward tumors. Rat liver epithelial (RLE) cells have both progenitor and stem cell-like properties. Therefore, our study elucidated the therapeutic effect of RLE cells in rat hepatoma cells. RLE cells were isolated from 10-day old rats and characterized for stem cell marker expression. RLE cells and rat hepatoma cells (H4-IIE-C3 cells) were co-cultured and divided into four groups with different ratios of RLE and hepatoma cells. Group A had only rat hepatoma cells as a control group. The ratios of rat hepatoma and RLE cells in group B, C and D were 5:1, 1:1 and 1:5, respectively. Effective inhibition of cell proliferation and migration was found in group D when compared to group A. There was a significant decrease in Bcl2 expression and increase in late apoptosis of rat hepatoma cells when adding more RLE cells. RLE cells reduced cell proliferation and migration of rat hepatoma cells. These results suggested that RLE cells could be used as a potential cell therapy.
authors.affiliation: Department of Surgery, Far Eastern Memorial Hospital, New Taipei City, Taiwan, R.O.C.
authors.forename: Kuo-Shyang
authors.initials: KS
authors.lastname: Jeng
authors.affiliation: Graduate Institute of Clinical Medicine, National Taiwan University, Taipei, Taiwan, R.O.C.
authors.forename: Chi-Juei
authors.initials: CJ
authors.lastname: Jeng
authors.affiliation: Department of Hepato-Gastroenterology, Chang-Gung Memorial Hospital, LinKou Medical Center, Chang-Gung University, Taipei, Taiwan, R.O.C.
authors.forename: Wen-Juei
authors.initials: WJ
authors.lastname: Jeng
authors.affiliation: Department of Hepato-Gastroenterology, Chang-Gung Memorial Hospital, LinKou Medical Center, Chang-Gung University, Taipei, Taiwan, R.O.C.
authors.forename: I-Shyan
authors.initials: IS
authors.lastname: Sheen
authors.affiliation: Department of Medical Research, Far Eastern Memorial Hospital, New Taipei City, Taiwan, R.O.C.
authors.forename: Shih-Yun
authors.initials: SY
authors.lastname: Li
authors.affiliation: Department of Surgery, Far Eastern Memorial Hospital, New Taipei City, Taiwan, R.O.C.
authors.forename: Zih-Hang
authors.initials: ZH
authors.lastname: Hung
authors.affiliation: Department of Medical Research, Far Eastern Memorial Hospital, New Taipei City, Taiwan, R.O.C.
authors.forename: Hsin-I
authors.initials: HI
authors.lastname: Hsiau
authors.affiliation: Department of Medical Research, Far Eastern Memorial Hospital, New Taipei City, Taiwan, R.O.C.
authors.forename: Ming-Che
authors.initials: MC
authors.lastname: Yu
authors.affiliation: Department of Surgery, Far Eastern Memorial Hospital, New Taipei City, Taiwan, R.O.C.
authors.forename: Chiung-Fang
authors.initials: CF
authors.lastname: Chang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000473:pathology / Q000628:therapy*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics*; D064987:Cell- and Tissue-Based Therapy; D018920:Coculture Techniques; D004847:Epithelial Cells / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic; D022781:Hepatocytes / Q000378:metabolism / Q000473:pathology; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000473:pathology / Q000628:therapy*; D014411:Neoplastic Stem Cells / Q000378:metabolism / Q000473:pathology; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis; D051381:Rats
chemical_list: D019253:Proto-Oncogene Proteins c-bcl-2