title: One-pot preparation of labelled mannan-peptide conjugate, model for immune cell processing.
abstract: An efficient method for preparation of fluorescently labelled mannan-peptide glycoconjugates has been developed. After selective Dess-Martin periodinane oxidation of mannan, it was conjugated to the fluorescent label alone and a peptide with the label via reductive amination. Prepared glycoconjugates were characterised by HPSEC, FTIR-ATR and UV-VIS spectroscopy. Finally, the fluorescently labelled mannan and mannan-peptide conjugate were used for microscopic visualization of their accumulation in intracellular organelles of RAW 264.7 cells.
authors.affiliation: Institute of Chemistry, Center for Glycomics, Slovak Academy of Sciences, Dúbravská cesta 9, 845 38, Bratislava, Slovak Republic. chempalo@savba.sk.
authors.forename: Pavol
authors.initials: P
authors.lastname: Farkaš
authors.affiliation: Institute of Chemistry, Center for Glycomics, Slovak Academy of Sciences, Dúbravská cesta 9, 845 38, Bratislava, Slovak Republic.
authors.forename: Alžbeta
authors.initials: A
authors.lastname: Čížová
authors.affiliation: Institute of Chemistry, Center for Glycomics, Slovak Academy of Sciences, Dúbravská cesta 9, 845 38, Bratislava, Slovak Republic.
authors.forename: Peter
authors.initials: P
authors.lastname: Bystrický
authors.affiliation: Institute of Chemistry, Center for Glycomics, Slovak Academy of Sciences, Dúbravská cesta 9, 845 38, Bratislava, Slovak Republic.
authors.forename: Lucia
authors.initials: L
authors.lastname: Paulovičová
authors.affiliation: Institute of Chemistry, Center for Glycomics, Slovak Academy of Sciences, Dúbravská cesta 9, 845 38, Bratislava, Slovak Republic.
authors.forename: Ema
authors.initials: E
authors.lastname: Paulovičová
authors.affiliation: Institute of Chemistry, Center for Glycomics, Slovak Academy of Sciences, Dúbravská cesta 9, 845 38, Bratislava, Slovak Republic.
authors.forename: Slavomír
authors.initials: S
authors.lastname: Bystrický
mesh_terms: D000818:Animals; D002175:Candida / Q000737:chemistry; D002460:Cell Line; D062610:Fungal Polysaccharides / Q000737:chemistry* / Q000276:immunology; D008264:Macrophages / Q000276:immunology; D051379:Mice; D010455:Peptides / Q000737:chemistry* / Q000276:immunology; D018074:Vaccines, Conjugate / Q000737:chemistry* / Q000276:immunology
chemical_list: D062610:Fungal Polysaccharides; D010455:Peptides; D018074:Vaccines, Conjugate
keywords: 2-aminobenzamide; Candida albicans; Fluorescent label; Glycoconjugate; Mannan