title: Esrrb-Cre excises loxP-flanked alleles in early four-cell embryos.
abstract: Among transgenic mice with ubiquitous Cre recombinase activity, all strains to date excise loxP-flanked (floxed) alleles either at or before the zygote stage or at nondescript stages of development. This manuscript describes a new mouse strain, in which Cre recombinase, integrated into the Esrrb locus, efficiently excises floxed alleles in pre-implantation embryos at the onset of the four-cell stage. By enabling inactivation of genes only after the embryo has undergone two cleavages, this strain should facilitate in vivo studies of genes with essential gametic or zygotic functions. In addition, this study describes a new, highly pluripotent hybrid C57BL/6J x 129S1/SvImJ mouse embryonic stem cell line, HYB12, in which this knockin and additional targeted alleles have been generated.
authors.affiliation: Magee-Womens Research Institute, Department of Obstetrics, Gynecology and Reproductive Sciences, University of Pittsburgh, 204 Craft Ave., Pittsburgh, PA 15213.
authors.forename: Sungeun
authors.initials: S
authors.lastname: Lee
authors.affiliation: Magee-Womens Research Institute, Department of Obstetrics, Gynecology and Reproductive Sciences, University of Pittsburgh, 204 Craft Ave., Pittsburgh, PA 15213.
authors.forename: Srinivas R
authors.initials: SR
authors.lastname: Pallerla
authors.affiliation: Magee-Womens Research Institute, Department of Obstetrics, Gynecology and Reproductive Sciences, University of Pittsburgh, 204 Craft Ave., Pittsburgh, PA 15213.
authors.forename: Suyeon
authors.initials: S
authors.lastname: Kim
authors.affiliation: Magee-Womens Research Institute, Department of Obstetrics, Gynecology and Reproductive Sciences, University of Pittsburgh, 204 Craft Ave., Pittsburgh, PA 15213.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Shaffer
authors.affiliation: Magee-Womens Research Institute, Department of Obstetrics, Gynecology and Reproductive Sciences, University of Pittsburgh, 204 Craft Ave., Pittsburgh, PA 15213.
authors.forename: Calvin R
authors.initials: CR
authors.lastname: Simerly
authors.affiliation: Magee-Womens Research Institute, Department of Obstetrics, Gynecology and Reproductive Sciences, University of Pittsburgh, 204 Craft Ave., Pittsburgh, PA 15213.
authors.forename: J
authors.initials: J
authors.lastname: Richard Chaillet
authors.affiliation: Magee-Womens Research Institute, Department of Obstetrics, Gynecology and Reproductive Sciences, University of Pittsburgh, 204 Craft Ave., Pittsburgh, PA 15213.
authors.forename: Yaacov
authors.initials: Y
authors.lastname: Barak
mesh_terms: D000483:Alleles; D000818:Animals; D002460:Cell Line; D017353:Gene Deletion; D018014:Gene Transfer Techniques; D019426:Integrases / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D011960:Receptors, Estrogen / Q000235:genetics*; D011995:Recombination, Genetic; D014764:Viral Proteins / Q000235:genetics; D015053:Zygote / Q000378:metabolism
chemical_list: C098925:Esrrb protein, mouse; D011960:Receptors, Estrogen; D014764:Viral Proteins; C045073:Cre recombinase; D019426:Integrases
keywords: Cre recombinase; Esrrb; four-cell embryos; hybrid ES cells; knockin