title: Genetic Mapping of Resistance to Meloidogyne arenaria in Arachis stenosperma: A New Source of Nematode Resistance for Peanut.
abstract: Root-knot nematodes (RKN; Meloidogyne sp.) are a major threat to crops in tropical and subtropical regions worldwide. The use of resistant crop varieties is the preferred method of control because nematicides are expensive, and hazardous to humans and the environment. Peanut (Arachis hypogaea) is infected by four species of RKN, the most damaging being M. arenaria, and commercial cultivars rely on a single source of resistance. In this study, we genetically characterize RKN resistance of the wild Arachis species A. stenosperma using a population of 93 recombinant inbred lines developed from a cross between A. duranensis and A. stenosperma. Four quantitative trait loci (QTL) located on linkage groups 02, 04, and 09 strongly influenced nematode root galling and egg production. Drought-related, domestication and agronomically relevant traits were also evaluated, revealing several QTL. Using the newly available Arachis genome sequence, easy-to-use KASP (kompetitive allele specific PCR) markers linked to the newly identified RKN resistance loci were developed and validated in a tetraploid context. Therefore, we consider that A. stenosperma has high potential as a new source of RKN resistance in peanut breeding programs.
authors.affiliation: Embrapa Genetic Resources and Biotechnology, PqEB W5 Norte Final, Brasília, DF, 70770-917, Brazil Center for Applied Genetic Technologies, University of Georgia, Athens, Georgia 30602-6810 soraya.bertioli@embrapa.br.
authors.forename: Soraya C M
authors.initials: SC
authors.lastname: Leal-Bertioli
authors.affiliation: Embrapa Genetic Resources and Biotechnology, PqEB W5 Norte Final, Brasília, DF, 70770-917, Brazil.
authors.forename: Márcio C
authors.initials: MC
authors.lastname: Moretzsohn
authors.affiliation: Department of Nematology, University of California, Riverside, California 92521.
authors.forename: Philip A
authors.initials: PA
authors.lastname: Roberts
authors.affiliation: Center for Applied Genetic Technologies, University of Georgia, Athens, Georgia 30602-6810.
authors.forename: Carolina
authors.initials: C
authors.lastname: Ballén-Taborda
authors.affiliation: Embrapa Rice and Beans, Rodovia GO-462, km 12 Zona Rural C.P. 179, Santo Antônio de Goiás, GO, 75375-000, Brazil.
authors.forename: Tereza C O
authors.initials: TC
authors.lastname: Borba
authors.affiliation: Embrapa Rice and Beans, Rodovia GO-462, km 12 Zona Rural C.P. 179, Santo Antônio de Goiás, GO, 75375-000, Brazil.
authors.forename: Paula A
authors.initials: PA
authors.lastname: Valdisser
authors.affiliation: Embrapa Rice and Beans, Rodovia GO-462, km 12 Zona Rural C.P. 179, Santo Antônio de Goiás, GO, 75375-000, Brazil.
authors.forename: Rosana P
authors.initials: RP
authors.lastname: Vianello
authors.affiliation: Embrapa Genetic Resources and Biotechnology, PqEB W5 Norte Final, Brasília, DF, 70770-917, Brazil.
authors.forename: Ana Cláudia G
authors.initials: AC
authors.lastname: Araújo
authors.affiliation: Embrapa Genetic Resources and Biotechnology, PqEB W5 Norte Final, Brasília, DF, 70770-917, Brazil.
authors.forename: Patricia M
authors.initials: PM
authors.lastname: Guimarães
authors.affiliation: Center for Applied Genetic Technologies, University of Georgia, Athens, Georgia 30602-6810 University of Brasília, Institute of Biological Sciences, Campus Darcy Ribeiro, Brasília, DF, 70910-900, Brazil.
authors.forename: David J
authors.initials: DJ
authors.lastname: Bertioli
mesh_terms: D000818:Animals; D010367:Arachis / Q000235:genetics* / Q000469:parasitology*; D002874:Chromosome Mapping; D060467:Disease Resistance / Q000235:genetics*; D055864:Droughts; D005819:Genetic Markers; D005828:Genetics, Population; D018745:Genome, Plant; D005838:Genotype; D010641:Phenotype; D010935:Plant Diseases / Q000235:genetics* / Q000469:parasitology*; D020641:Polymorphism, Single Nucleotide; D011123:Polyploidy; D040641:Quantitative Trait Loci; D019655:Quantitative Trait, Heritable; D015203:Reproducibility of Results; D013312:Stress, Physiological; D014431:Tylenchoidea
chemical_list: D005819:Genetic Markers
keywords: Arachis; QTL; drought; introgression; marker-assisted selection; peanut; root-knot nematode resistance; yield