title: Destructive effect of anticancer oxali-palladium on heme degradation through the generation of endogenous hydrogen peroxide.
abstract: The interaction between human hemoglobin (Hb) and oxali-palladium was studied using different spectroscopic methods of UV-vis, fluorescence, circular dichroism (CD), and chemiluminescence at two temperatures of 25 and 37°C. The experimental results showed that both dynamic and static quenching is occurred simultaneously when oxali-palladium quenches the fluorescence of Hb. According to the fluorescence quenching method, the binding site number, apparent binding constant, and corresponding thermodynamic parameters were measured at two temperatures. The values of ΔH°, ΔS°, and ΔG° indicate that process of the formation of oxali-palladium-Hb complex is a spontaneous interaction procedure in which electrostatic interaction plays a major role. In addition, UV-vis and CD results showed that the addition of oxali-palladium changes the conformation of Hb. To evaluate the functional changes of Hb via destruction of the heme structure, fluorescence studies were performed. The results demonstrated that two fluorescent heme degradation products are found during the interaction of oxali-palladium with Hb. Also, the amount of hydrogen peroxide produced in the solution of Hb due to the interaction of oxali-palladium with Hb using chemiluminescence method indicated heme degradation in the protein is occurred. Structural and functional changes induced in Hb via heme degradation are considered as side effects of this synthesized anticancer drug.
authors.affiliation: a Department of Cell & Molecular Biology, Faculty of Biological Sciences , Kharazmi University , Tehran , Iran.
authors.forename: K
authors.initials: K
authors.lastname: Abbasi-Tajarag
authors.affiliation: a Department of Cell & Molecular Biology, Faculty of Biological Sciences , Kharazmi University , Tehran , Iran.
authors.forename: A
authors.initials: A
authors.lastname: Divsalar
authors.affiliation: b Institute of Biochemistry and Biophysics , University of Tehran , Tehran , Iran.|c Center of Excellence in Biothermodynamics , University of Tehran , Tehran , Iran.
authors.forename: A A
authors.initials: AA
authors.lastname: Saboury
authors.affiliation: d Department of Medical Nanotechnology , Science and Research Branch, Islamic Azad University , Tehran , Iran.
authors.forename: B
authors.initials: B
authors.lastname: Ghalandari
authors.affiliation: b Institute of Biochemistry and Biophysics , University of Tehran , Tehran , Iran.
authors.forename: H
authors.initials: H
authors.lastname: Ghourchian
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry* / Q000378:metabolism; D002942:Circular Dichroism; D006418:Heme / Q000737:chemistry* / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000737:chemistry* / Q000378:metabolism; D008163:Luminescent Measurements; D008968:Molecular Conformation; D062105:Molecular Docking Simulation; D056004:Molecular Dynamics Simulation; D009942:Organometallic Compounds / Q000737:chemistry* / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013816:Thermodynamics
chemical_list: D000970:Antineoplastic Agents; D009942:Organometallic Compounds; D006418:Heme; D006861:Hydrogen Peroxide
keywords: chemiluminescence; heme degradation; human hemoglobin; oxali-palladium