title: Serum amyloid a gene polymorphism and its association with lipid profile in Saudi females with osteoporosis.
abstract: OBJECTIVE
  Osteoporosis can be defined as a systemic skeletal disease characterized by low bone mass and micro architectural decline of bone tissue. Serum amyloid A (SAA) is a family of protein that increases up to 1,000-fold in blood during inflammation. In this study, we aimed to study the relationship between SAA1 gene polymorphism (rs12218) and lipid profile and osteoporosis.
  
  
  METHODS
  The study was performed on the female students of Taibah University in Al Medina, KSA during June 2014 to April 2015. According to BMD; osteoporosis group (138 students) and control group (128 students). All groups were subjected to; BMI, BMD, calcium, phosphorus, creatinine, lipid profile and SAA. Polymerase chain reaction and Real Time were done to determine the distribution of allele and genotype frequency of SAA (rs12218) C/T polymorphism.
  
  
  RESULTS
  This study shows that the TT genotype of rs12218 was more frequent in osteoporosis group than control group (P<0.001). Also, TT genotype and T allel was found to be associated with plasma total cholesterol, TG, LDLc, HDLc, Tscore, Zscore and SAA1 level in osteoporosis group (P=0.000, P=0.05, and P=0.000, P=0.000, P=0.01, P=0.02, P=0.000 respectively). The logistic regression analysis with and without lipid disorders in the osteoporosis group also show that the TT genotype of rs12218 still differed significantly between these two groups (P=0.001, OR=1.814, 95% CI: 0.719-4.577).
  
  
  CONCLUSIONS
  The results of this study shows a significant association between TT genotype of rs12218 and both lipid level and osteoporosis in Saudi female population.
authors.affiliation: Azza M. Abdu-Allah MD. Menofyia University, Egypt. Department of Biochemistry and Molecular Medicine, Faculty of Medicine, Taibah University, Saudi Arabia.
authors.forename: Azza M
authors.initials: AM
authors.lastname: Abdu-Allah
authors.affiliation: Shereen A. El Tarhouny MD. Zagazig University, Egypt. Department of Biochemistry and Molecular Medicine, Faculty of Medicine, Taibah University, Saudi Arabia.
authors.forename: Shereen A El
authors.initials: SA
authors.lastname: Tarhouny
authors.affiliation: Hussam Hussein Baghdadi, PhD. Department of Biochemistry and Molecular Medicine, Faculty of Medicine, Taibah University, Saudi Arabia.
authors.forename: Hussam Hussein
authors.initials: HH
authors.lastname: Baghdadi
keywords: Allel; Genotyping; Osteoporosis; Real time; SAA1