title: Correlation between apoptosis and left ventricular remodeling in subacute phase of myocardial ischemia and reperfusion.
abstract: BACKGROUND
  To investigate whether an apoptotic process demonstrated by (99m)Tc-annexin-V ((99m)Tc-AV) uptake correlates with left ventricular remodeling (LVR) after myocardial infarction, we assessed (99m)Tc-AV uptake in rat model of myocardial ischemia and reperfusion.
  
  
  METHODS
  The left coronary artery (LCA) of 15 rats was occluded for 20 to 30 min, followed by reperfusion. After 2 weeks, (99m)Tc-AV was injected, and then 1 h later, (201)Tl was injected after reocclusion of the LCA. Dual-tracer autoradiography was performed to assess (99m)Tc-AV uptake and the area at risk (AAR) by (201)Tl defect. (99m)Tc-AV uptake ratio was calculated by dividing the count density of the AAR by that of the normally perfused area. In short-axis LV slices, LV cavity dilation index (DI) was calculated by dividing the area of LV cavity by that of the whole LV area. LV wall-thinning ratio (WTR) was calculated by dividing the LV wall thickness in the AAR by that of the normally perfused area.
  
  
  RESULTS
  Significant (99m)Tc-AV uptake in the AAR was observed in 10 rats. DI was significantly higher in rats with positive (99m)Tc-AV uptake than in rats without uptake. WTR was smaller in rats with positive (99m)Tc-AV uptake than in rats without uptake.
  
  
  CONCLUSIONS
  The data suggest (99m)Tc-AV uptake in injured myocardium might correlate with LVR at 2 weeks after myocardial ischemia and reperfusion.
authors.affiliation: Department of Nuclear Medicine, Kanazawa University Hospital, 13-1 Takara-machi, Kanazawa, Ishikawa, 920-8641, Japan. hiroshi19810318@yahoo.co.jp.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Wakabayashi
authors.affiliation: Department of Nuclear Medicine, Kanazawa University Hospital, 13-1 Takara-machi, Kanazawa, Ishikawa, 920-8641, Japan.
authors.forename: Junichi
authors.initials: J
authors.lastname: Taki
authors.affiliation: Department of Nuclear Medicine, Kanazawa University Hospital, 13-1 Takara-machi, Kanazawa, Ishikawa, 920-8641, Japan.
authors.forename: Anri
authors.initials: A
authors.lastname: Inaki
authors.affiliation: Division of Tracer Kinetics, Advanced Science Research Centre, Kanazawa University, 13-1 Takara-machi, Kanazawa, 920-8640, Japan.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Shiba
authors.affiliation: The Medical and Pharmacological Research Centre Foundation, Wo 32, Inoyama, Hakui, 925-0613, Japan.
authors.forename: Ichiro
authors.initials: I
authors.lastname: Matsunari
authors.affiliation: Department of Nuclear Medicine, Kanazawa University Hospital, 13-1 Takara-machi, Kanazawa, Ishikawa, 920-8641, Japan.
authors.forename: Seigo
authors.initials: S
authors.lastname: Kinuya
keywords: 99mTc-annexin-V; Apoptosis; Myocardial ischemia; Myocardial remodeling