title: Cross-species conservation of complementary amino acid-ribonucleobase interactions and their potential for ribosome-free encoding.
abstract: The role of amino acid-RNA nucleobase interactions in the evolution of RNA translation and protein-mRNA autoregulation remains an open area of research. We describe the inference of pairwise amino acid-RNA nucleobase interaction preferences using structural data from known RNA-protein complexes. We observed significant matching between an amino acid's nucleobase affinity and corresponding codon content in both the standard genetic code and mitochondrial variants. Furthermore, we showed that knowledge of nucleobase preferences allows statistically significant prediction of protein primary sequence from mRNA using purely physiochemical information. Interestingly, ribosomal primary sequences were more accurately predicted than non-ribosomal sequences, suggesting a potential role for direct amino acid-nucleobase interactions in the genesis of amino acid-based ribosomal components. Finally, we observed matching between amino acid-nucleobase affinities and corresponding mRNA sequences in 35 evolutionarily diverse proteomes. We believe these results have important implications for the study of the evolutionary origins of the genetic code and protein-mRNA cross-regulation.
authors.affiliation: Department of Biology, Carleton College, 1 College Street, Northfield MN, 55057, United States.
authors.forename: John G D
authors.initials: JG
authors.lastname: Cannon
authors.affiliation: Department of Biology, Harvey Mudd College, 301 Platt Blvd, Claremont CA 91711, United States.|Department of Computer Science, Harvey Mudd College, 301 Platt Blvd, Claremont CA 91711, United States.
authors.forename: Rachel M
authors.initials: RM
authors.lastname: Sherman
authors.affiliation: Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge, CB2 1GA, United Kingdom.
authors.forename: Victoria M Y
authors.initials: VM
authors.lastname: Wang
authors.affiliation: Department of Mathematics, Carleton College, 1 College Street, Northfield MN, 55057, United States.
authors.forename: Grace A
authors.initials: GA
authors.lastname: Newman
mesh_terms: D000596:Amino Acids / Q000378:metabolism*; D003062:Codon / Q000235:genetics / Q000378:metabolism; D005815:Genetic Code / Q000235:genetics; D006801:Humans; D009696:Nucleic Acids / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism; D012313:RNA / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism*
chemical_list: D000596:Amino Acids; D003062:Codon; D009696:Nucleic Acids; D011506:Proteins; D012333:RNA, Messenger; D012313:RNA