title: Myogenic Differentiation of Mouse Embryonic Stem Cells That Lack a Functional Pax7 Gene.
abstract: The transcription factor Pax7 plays a key role during embryonic myogenesis and sustains the proper function of satellite cells, which serve as adult skeletal muscle stem cells. Overexpression of Pax7 has been shown to promote the myogenic differentiation of pluripotent stem cells. However, the effects of the absence of functional Pax7 in differentiating embryonic stem cells (ESCs) have not yet been directly tested. Herein, we studied mouse stem cells that lacked a functional Pax7 gene and characterized the differentiation of these stem cells under conditions that promoted the derivation of myoblasts in vitro. We analyzed the expression of myogenic factors, such as myogenic regulatory factors and muscle-specific microRNAs, in wild-type and mutant cells. Finally, we compared the transcriptome of both types of cells and did not find substantial differences in the expression of genes related to the regulation of myogenesis. As a result, we showed that the absence of functional Pax7 does not prevent the in vitro myogenic differentiation of ESCs.
authors.affiliation: 1 Department of Cytology, Faculty of Biology, Institute of Zoology, University of Warsaw , Warsaw, Poland .
authors.forename: Areta M
authors.initials: AM
authors.lastname: Czerwinska
authors.affiliation: 1 Department of Cytology, Faculty of Biology, Institute of Zoology, University of Warsaw , Warsaw, Poland .
authors.forename: Iwona
authors.initials: I
authors.lastname: Grabowska
authors.affiliation: 1 Department of Cytology, Faculty of Biology, Institute of Zoology, University of Warsaw , Warsaw, Poland .
authors.forename: Karolina
authors.initials: K
authors.lastname: Archacka
authors.affiliation: 1 Department of Cytology, Faculty of Biology, Institute of Zoology, University of Warsaw , Warsaw, Poland .
authors.forename: Joanna
authors.initials: J
authors.lastname: Bem
authors.affiliation: 1 Department of Cytology, Faculty of Biology, Institute of Zoology, University of Warsaw , Warsaw, Poland .
authors.forename: Barbara
authors.initials: B
authors.lastname: Swierczek
authors.affiliation: 1 Department of Cytology, Faculty of Biology, Institute of Zoology, University of Warsaw , Warsaw, Poland .
authors.forename: Anita
authors.initials: A
authors.lastname: Helinska
authors.affiliation: 1 Department of Cytology, Faculty of Biology, Institute of Zoology, University of Warsaw , Warsaw, Poland .
authors.forename: Wladyslawa
authors.initials: W
authors.lastname: Streminska
authors.affiliation: 2 Laboratory of Microarray Analysis, Institute of Biochemistry and Biophysics , Polish Academy of Sciences, Warsaw, Poland .
authors.forename: Anna
authors.initials: A
authors.lastname: Fogtman
authors.affiliation: 2 Laboratory of Microarray Analysis, Institute of Biochemistry and Biophysics , Polish Academy of Sciences, Warsaw, Poland .|3 Department of Systems Biology, Faculty of Biology, University of Warsaw , Warsaw, Poland .
authors.forename: Roksana
authors.initials: R
authors.lastname: Iwanicka-Nowicka
authors.affiliation: 2 Laboratory of Microarray Analysis, Institute of Biochemistry and Biophysics , Polish Academy of Sciences, Warsaw, Poland .|3 Department of Systems Biology, Faculty of Biology, University of Warsaw , Warsaw, Poland .
authors.forename: Marta
authors.initials: M
authors.lastname: Koblowska
authors.affiliation: 1 Department of Cytology, Faculty of Biology, Institute of Zoology, University of Warsaw , Warsaw, Poland .
authors.forename: Maria A
authors.initials: MA
authors.lastname: Ciemerych
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics*; D005260:Female; D018507:Gene Expression Regulation, Developmental; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D035683:MicroRNAs / Q000378:metabolism; D000066450:Mouse Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism*; D024510:Muscle Development / Q000235:genetics*; D051763:PAX7 Transcription Factor / Q000235:genetics* / Q000378:metabolism
chemical_list: D035683:MicroRNAs; D051763:PAX7 Transcription Factor