title: B'-protein phosphatase 2A is a functional binding partner of delta-retroviral integrase.
abstract: To establish infection, a retrovirus must insert a DNA copy of its RNA genome into host chromatin. This reaction is catalysed by the virally encoded enzyme integrase (IN) and is facilitated by viral genus-specific host factors. Herein, cellular serine/threonine protein phosphatase 2A (PP2A) is identified as a functional IN binding partner exclusive to δ-retroviruses, including human T cell lymphotropic virus type 1 and 2 (HTLV-1 and HTLV-2) and bovine leukaemia virus (BLV). PP2A is a heterotrimer composed of a scaffold, catalytic and one of any of four families of regulatory subunits, and the interaction is specific to the B' family of the regulatory subunits. B'-PP2A and HTLV-1 IN display nuclear co-localization, and the B' subunit stimulates concerted strand transfer activity of δ-retroviral INs in vitro. The protein-protein interaction interface maps to a patch of highly conserved residues on B', which when mutated render B' incapable of binding to and stimulating HTLV-1 and -2 IN strand transfer activity.
authors.affiliation: Division of Infectious Diseases, St. Mary's campus, Imperial College London, Norfolk Place, London, W2 1PG, UK g.maertens@imperial.ac.uk.
authors.forename: Goedele N
authors.initials: GN
authors.lastname: Maertens
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism; D002417:Cattle; D002460:Cell Line; D017977:Deltaretrovirus / Q000201:enzymology / Q000378:metabolism*; D004789:Enzyme Activation; D015368:Human T-lymphotropic virus 1 / Q000201:enzymology / Q000378:metabolism; D006801:Humans; D019426:Integrases / Q000378:metabolism*; D001909:Leukemia Virus, Bovine / Q000201:enzymology / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011485:Protein Binding; D011487:Protein Conformation; D054730:Protein Interaction Domains and Motifs; D025941:Protein Interaction Mapping; D054648:Protein Phosphatase 2 / Q000737:chemistry / Q000378:metabolism*; D021122:Protein Subunits; D016415:Sequence Alignment; D016662:Virus Integration
chemical_list: D002352:Carrier Proteins; D021122:Protein Subunits; D019426:Integrases; D054648:Protein Phosphatase 2