title: A Highly Sensitive Oligonucleotide Hybridization Assay for Klebsiella pneumoniae Carbapenemase with the Probes on a Gold Nanoparticles Modified Glassy Carbon Electrode.
abstract: To develop a new electrochemical DNA biosensor for determination of Klebsiella pneumoniae carbapenemase, a highly sensitive and selective electrochemical biosensor for DNA detection was constructed based on a glassy carbon electrode (GCE) modified with gold nanoparticles (Au-nano). The Au-nano/GCE was characterized by scanning electromicroscopy, cyclic voltammetry, and electrochemical impedance spectroscopy. The hybridization detection was measured by differential pulse voltammetry using methylene blue as the hybridization indicator. The dynamic range of detection of the sensor for the target DNA sequences was from 1 × 10(-11) to 1 × 10(-8) M, with an LOD of 1 × 10(-12) M. The DNA biosensor had excellent specificity for distinguishing complementary DNA sequence in the presence of non-complementary and mismatched DNA sequence. The Au-nano/GCE showed significant improvement in electrochemical characteristics, and this biosensor was successfully applied for determination of K. pneumoniae.
authors.affiliation: Public Health School, Harbin Medical University, No. 157 Baojian Rd, Nangang District, Harbin, Heilongjiang Province, 150081, People's Republic of China.
authors.forename: Hong-zhi
authors.initials: HZ
authors.lastname: Pan
authors.forename: Hong- Wei
authors.initials: H
authors.lastname: Yu
authors.forename: Na
authors.initials: N
authors.lastname: Wang
authors.forename: Ze
authors.initials: Z
authors.lastname: Zhang
authors.forename: Guang-Cai
authors.initials: GC
authors.lastname: Wan
authors.forename: Hao
authors.initials: H
authors.lastname: Liu
authors.forename: Xue
authors.initials: X
authors.lastname: Guan
authors.forename: Dong
authors.initials: D
authors.lastname: Chang
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis*; D015374:Biosensing Techniques / Q000379:methods*; D002244:Carbon; D004563:Electrochemistry; D004566:Electrodes; D006046:Gold; D007711:Klebsiella pneumoniae / Q000201:enzymology*; D053768:Metal Nanoparticles / Q000737:chemistry*; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000235:genetics*; D001618:beta-Lactamases / Q000032:analysis*
chemical_list: D001426:Bacterial Proteins; D009841:Oligonucleotides; D002244:Carbon; D006046:Gold; D001618:beta-Lactamases; C063912:carbapenemase