title: Intravenous Single-Dose Toxicity of Redaporfin-Based Photodynamic Therapy in Rodents.
abstract: We assessed the tolerability and safety in rodents of a single intravenous (i.v.) dose of redaporfin, a novel photosensitizer for Photodynamic Therapy (PDT) of cancer. Two approaches were used to evaluate acute toxicity: (i) a dose escalation study in BALB/c mice to evaluate the maximum tolerated dose of redaporfin; and (ii) a safety toxicology study in Wistar rats, of a single dose of redaporfin, with or without illumination, to evaluate possible signs of systemic toxicity. Redaporfin formulation was well tolerated by mice, with no signs of adverse reactions up to 75 mg/kg. In rats, there were no relevant changes, except for a significant, but transient, increase in the blood serum markers for hepatic function and muscle integrity, and also on neutrophil counts, observed after the application of light. The overall results showed that redaporfin-PDT is very well tolerated. No abnormalities were observed, including reactions at the injection site or skin phototoxicity, although the animals were maintained in normal indoor lighting. Redaporfin also showed a high efficacy in the treatment of male BALB/c mice with subcutaneously implanted colon (CT26) tumours. Vascular-PDT with 1.5 mg/kg redaporfin and a light dose of 74 J/cm² led to the complete tumour regression in 83% of the mice.
authors.affiliation: Luzitin SA, S. Martinho do Bispo, Coimbra 3045-016, Portugal. lrocha@luzitin.pt.|Bluepharma-Indústria Farmacêutica SA, S. Martinho do Bispo, Coimbra 3045-016, Portugal. lrocha@luzitin.pt.
authors.forename: Luis B
authors.initials: LB
authors.lastname: Rocha
authors.affiliation: Luzitin SA, S. Martinho do Bispo, Coimbra 3045-016, Portugal. fschaberle@luzitin.pt.
authors.forename: Fábio
authors.initials: F
authors.lastname: Schaberle
authors.affiliation: Faculty of Chemistry, Jagiellonian University, Krakow 30-060, Poland. jdabrows@chemia.uj.edu.pl.
authors.forename: Janusz M
authors.initials: JM
authors.lastname: Dąbrowski
authors.affiliation: Bluepharma-Indústria Farmacêutica SA, S. Martinho do Bispo, Coimbra 3045-016, Portugal. ssimoes@bluepharma.pt.
authors.forename: Sérgio
authors.initials: S
authors.lastname: Simões
authors.affiliation: Chemistry Department, University of Coimbra, Coimbra 3004-535, Portugal. lgarnaut@ci.uc.pt.
authors.forename: Luis G
authors.initials: LG
authors.lastname: Arnaut
mesh_terms: D061605:Administration, Intravenous; D000818:Animals; D015415:Biomarkers; D001835:Body Weight / Q000187:drug effects; D005260:Female; D008297:Male; D051379:Mice; D010778:Photochemotherapy / Q000379:methods; D017319:Photosensitizing Agents / Q000008:administration & dosage / Q000494:pharmacology* / Q000633:toxicity; D011166:Porphyrins / Q000494:pharmacology*; D051381:Rats; D013449:Sulfonamides / Q000494:pharmacology*; D018675:Toxicity Tests
chemical_list: D015415:Biomarkers; D017319:Photosensitizing Agents; D011166:Porphyrins; D013449:Sulfonamides; C000610760:redaporfin
keywords: bacteriochlorin; cancer treatment; intravenous formulation; photodynamic therapy; redaporfin; rodents; single-dose toxicity