title: A high sensitive label-free immunosensor for the determination of human serum IgG using overoxidized polypyrrole decorated with gold nanoparticle modified electrode.
abstract: Herein, an electrochemical human immunoglobulin G (HIgG) immunosensor was fabricated using overoxidized polypyrrole decorated with gold nanoparticle (OPPy-Aunano) modified screen printed electrode (OPPy-Aunano/SPE). The results clearly demonstrate that OPPy-Aunano film is very permeable to [Fe(CN)6](3-/4-). Therefore, the fabrication of immunosensor based on permeability of OPPy-Aunano film to [Fe(CN)6](3-/4-) probe is a novel strategy for the fabrication of label free electrochemical immunosensor. The electrochemical impedance spectroscopy (EIS) techniques are used for the determination of HIgG in the concentration range 0.5 to 125.0ngmL(-1) and with a limit of detection of 0.02ngmL(-1), respectively.
authors.affiliation: Department of Chemistry, Razi University, Kermanshah, Iran; Nano Drug Delivery Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran.
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Amouzadeh Tabrizi
authors.affiliation: Department of Chemistry, Razi University, Kermanshah, Iran. Electronic address: mshamsipur@yahoo.com.
authors.forename: Mojtaba
authors.initials: M
authors.lastname: Shamsipur
authors.affiliation: Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Mostafaie
mesh_terms: D004867:Equipment Design; D006046:Gold / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods; D007074:Immunoglobulin G / Q000097:blood*; D053768:Metal Nanoparticles / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry*
chemical_list: D007074:Immunoglobulin G; D011108:Polymers; D011758:Pyrroles; C067635:polypyrrole; D006046:Gold
keywords: EIS; HIgG; Immunosensor; Label-free method; OPPy-Au(nano)