title: Anhydride-functional silane immobilized onto titanium surfaces induces osteoblast cell differentiation and reduces bacterial adhesion and biofilm formation.
abstract: Bacterial infection in dental implants along with osseointegration failure usually leads to loss of the device. Bioactive molecules with antibacterial properties can be attached to titanium surfaces with anchoring molecules such as silanes, preventing biofilm formation and improving osseointegration. Properties of silanes as molecular binders have been thoroughly studied, but research on the biological effects of these coatings is scarce. The aim of the present study was to determine the in vitro cell response and antibacterial effects of triethoxysilypropyl succinic anhydride (TESPSA) silane anchored on titanium surfaces. X-ray photoelectron spectroscopy confirmed a successful silanization. The silanized surfaces showed no cytotoxic effects. Gene expression analyses of Sarcoma Osteogenic (SaOS-2) osteoblast-like cells cultured on TESPSA silanized surfaces reported a remarkable increase of biochemical markers related to induction of osteoblastic cell differentiation. A manifest decrease of bacterial adhesion and biofilm formation at early stages was observed on treated substrates, while favoring cell adhesion and spreading in bacteria-cell co-cultures. Surfaces treated with TESPSA could enhance a biological sealing on implant surfaces against bacteria colonization of underlying tissues. Furthermore, it can be an effective anchoring platform of biomolecules on titanium surfaces with improved osteoblastic differentiation and antibacterial properties.
authors.affiliation: Biomaterials, Biomechanics and Tissue Engineering Group, Department of Materials Science and Metallurgy, Technical University of Catalonia (UPC), ETSEIB, Av. Diagonal 647, 08028 Barcelona, Spain; Centre for Research in NanoEngineering (CRNE) - UPC, C/ Pascual i Vila 15, 08028 Barcelona, Spain. Electronic address: maria.godoy.gallardo@upc.edu.
authors.forename: Maria
authors.initials: M
authors.lastname: Godoy-Gallardo
authors.affiliation: Biomaterials, Biomechanics and Tissue Engineering Group, Department of Materials Science and Metallurgy, Technical University of Catalonia (UPC), ETSEIB, Av. Diagonal 647, 08028 Barcelona, Spain; Centre for Research in NanoEngineering (CRNE) - UPC, C/ Pascual i Vila 15, 08028 Barcelona, Spain. Electronic address: jordi.guillem.marti@upc.edu.
authors.forename: Jordi
authors.initials: J
authors.lastname: Guillem-Marti
authors.affiliation: Department of Mechanics, Escola Universitària Salesiana de Sarrià (EUSS), C/ Passeig de Sant Bosco, 42, 08017 Barcelona, Spain. Electronic address: psevilla@euss.es.
authors.forename: Pablo
authors.initials: P
authors.lastname: Sevilla
authors.affiliation: Biomaterials, Biomechanics and Tissue Engineering Group, Department of Materials Science and Metallurgy, Technical University of Catalonia (UPC), ETSEIB, Av. Diagonal 647, 08028 Barcelona, Spain; Centre for Research in NanoEngineering (CRNE) - UPC, C/ Pascual i Vila 15, 08028 Barcelona, Spain. Electronic address: jose.maria.manero@upc.edu.
authors.forename: José M
authors.initials: JM
authors.lastname: Manero
authors.affiliation: Biomaterials, Biomechanics and Tissue Engineering Group, Department of Materials Science and Metallurgy, Technical University of Catalonia (UPC), ETSEIB, Av. Diagonal 647, 08028 Barcelona, Spain; Centre for Research in NanoEngineering (CRNE) - UPC, C/ Pascual i Vila 15, 08028 Barcelona, Spain. Electronic address: francesc.xavier.gil@upc.edu.
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Gil
authors.affiliation: Biomaterials, Biomechanics and Tissue Engineering Group, Department of Materials Science and Metallurgy, Technical University of Catalonia (UPC), ETSEIB, Av. Diagonal 647, 08028 Barcelona, Spain; Centre for Research in NanoEngineering (CRNE) - UPC, C/ Pascual i Vila 15, 08028 Barcelona, Spain. Electronic address: daniel.rodriguez.rius@upc.edu.
authors.forename: Daniel
authors.initials: D
authors.lastname: Rodriguez
mesh_terms: D001419:Bacteria / Q000187:drug effects; D001422:Bacterial Adhesion / Q000187:drug effects*; D018441:Biofilms / Q000187:drug effects*; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D006801:Humans; D010006:Osteoblasts / Q000187:drug effects*; D012821:Silanes / Q000737:chemistry* / Q000494:pharmacology; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D012821:Silanes; D014025:Titanium
keywords: Bacterial adhesion; Biofilm; Osteoblast differentiation; Silane; Titanium