title: SLC7A5 act as a potential leukemic transformation target gene in myelodysplastic syndrome.
abstract: OBJECTIVE
  Myelodysplastic syndromes (MDS) are a heterogenous group of clonal hematopoietic stem cell disorders characterized by increased risk of leukemic transformation. This study identifies microRNAs(miRNA) and miRNA targets that might represent leukemic transformation markers for MDS.
  
  
  METHODS
  Based on our previously established nested case-control study cohort of MDS patients, we chose paired patients to undergo Angilent 8 × 15K human miRNA microarrays. Target prediction analysis was administrated using targetscan 5.1 software. We further investigated the function of target gene in MDS cell line using siRNA method, including cell proliferation, cell apoptosis, cell cycle and electron microscope.
  
  
  RESULTS
  Finally we screened a subset of 7 miRNAs to be significantly differentially expressed between the case (at the end of follow up with leukemic transformation) and control group (at the end of follow up without leukemic transformation). Target prediction analysis revealed SLC7A5 was the common target gene of these 7 miRNAs. Further study on the function of SLC7A5 gene in SKM-1 cell line showed that downregulation of SLC7A5 inhibited SKM-1 cells proliferation, increased apoptosis and caused cell cycle arrest in the G0/G1 stage.
  
  
  CONCLUSIONS
  Our data indicate that SLC7A5 gene may act as a potential leukemic transformation target gene in MDS.
authors.affiliation: Department of Hematology, Huashan Hospital, Fudan University, Shanghai, 200040, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Department of Hematology, Huashan Hospital, Fudan University, Shanghai, 200040, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Song
authors.affiliation: Department of Hematology, Huashan Hospital, Fudan University, Shanghai, 200040, China.
authors.forename: Bobin
authors.initials: B
authors.lastname: Chen
authors.affiliation: Department of Hematology, Huashan Hospital, Fudan University, Shanghai, 200040, China.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Xu
authors.affiliation: Department of Hematology, Huashan Hospital, Fudan University, Shanghai, 200040, China.
authors.forename: Guowei
authors.initials: G
authors.lastname: Lin
mesh_terms: D000328:Adult; D000368:Aged; D026923:Amino Acid Transport Systems, Basic / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D017209:Apoptosis; D015153:Blotting, Western; D016022:Case-Control Studies; D002453:Cell Cycle; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000473:pathology*; D015331:Cohort Studies; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008854:Microscopy, Electron; D008875:Middle Aged; D009190:Myelodysplastic Syndromes / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
chemical_list: D026923:Amino Acid Transport Systems, Basic; D035683:MicroRNAs; D012333:RNA, Messenger; D034741:RNA, Small Interfering; C469951:SLC7A6 protein, human
keywords: SKM-1 cell line; SLC7A5 gene; miRNA; myelodysplastic syndrome; siRNA