title: Stelliosphaerols A and B, Sesquiterpene-Polyol Conjugates from an Ecuadorian Fungal Endophyte.
abstract: Endophytic fungi are plant tissue-associated fungi that represent a rich resource of unexplored biological and chemical diversity. As part of an ongoing effort to characterize Amazon rainforest-derived endophytes, numerous fungi were isolated and cultured from plants collected in the Yasuní National Park in Ecuador. Of these samples, phylogenetic and morphological data revealed a previously undescribed fungus in the order Pleosporales that was cultured from the tropical tree Duroia hirsuta. Extracts from this fungal isolate displayed activity against Staphylococcus aureus and were thus subjected to detailed chemical studies. Two compounds with modest antibacterial activity were isolated, and their structures were elucidated using a combination of NMR spectroscopic analysis, LC-MS studies, and chemical degradation. These efforts led to the identification of stelliosphaerols A (1) and B (2), new sesquiterpene-polyol conjugates that are responsible, at least in part, for the S. aureus inhibitory activity of the fungal extract.
authors.affiliation: Department of Molecular Biophysics and Biochemistry, Yale University , 260 Whitney Avenue, New Haven, Connecticut 06520-8114, United States.
authors.forename: Giovanni C
authors.initials: GC
authors.lastname: Forcina
authors.affiliation: Molecular Targets Laboratory, Center for Cancer Research, National Cancer Institute , Frederick, Maryland 21702-1201, United States.
authors.forename: Amaya
authors.initials: A
authors.lastname: Castro
authors.affiliation: Molecular Targets Laboratory, Center for Cancer Research, National Cancer Institute , Frederick, Maryland 21702-1201, United States.|Basic Science Program, Leidos Biomedical Research, Inc., Frederick National Laboratory , Frederick, Maryland 21702-1201, United States.
authors.forename: Heidi R
authors.initials: HR
authors.lastname: Bokesch
authors.affiliation: Department of Molecular Biophysics and Biochemistry, Yale University , 260 Whitney Avenue, New Haven, Connecticut 06520-8114, United States.
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Spakowicz
authors.affiliation: Department of Molecular Biophysics and Biochemistry, Yale University , 260 Whitney Avenue, New Haven, Connecticut 06520-8114, United States.
authors.forename: Michelle E
authors.initials: ME
authors.lastname: Legaspi
authors.affiliation: Department of Molecular Biophysics and Biochemistry, Yale University , 260 Whitney Avenue, New Haven, Connecticut 06520-8114, United States.
authors.forename: Kaury
authors.initials: K
authors.lastname: Kucera
authors.affiliation: Laboratorio de Biotecnología Vegetal, Pontificia Universidad Católica del Ecuador , Quito 17 01 21 84, Ecuador.
authors.forename: Stephany
authors.initials: S
authors.lastname: Villota
authors.affiliation: Laboratorio de Biotecnología Vegetal, Pontificia Universidad Católica del Ecuador , Quito 17 01 21 84, Ecuador.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Narváez-Trujillo
authors.affiliation: Molecular Targets Laboratory, Center for Cancer Research, National Cancer Institute , Frederick, Maryland 21702-1201, United States.
authors.forename: James B
authors.initials: JB
authors.lastname: McMahon
authors.affiliation: Molecular Targets Laboratory, Center for Cancer Research, National Cancer Institute , Frederick, Maryland 21702-1201, United States.
authors.forename: Kirk R
authors.initials: KR
authors.lastname: Gustafson
authors.affiliation: Department of Molecular Biophysics and Biochemistry, Yale University , 260 Whitney Avenue, New Haven, Connecticut 06520-8114, United States.
authors.forename: Scott A
authors.initials: SA
authors.lastname: Strobel
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D004484:Ecuador; D060026:Endophytes; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D011108:Polymers; D012717:Sesquiterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D011108:Polymers; D012717:Sesquiterpenes; C024617:polyol; C000607253:stelliosphaerol A; C000607254:stelliosphaerol B