title: Curcumin half analog modulates interleukin-6 and tumor necrosis factor-alpha in inflammatory bowel disease.
abstract: BACKGROUND
  The present study was aimed at examining the effect of dehydrozingerone (DHZ), half analogue of curcumin which is the active constituent of turmeric (Curcuma longa) in the di-nitrochlorobenzene (DNCB) induced model for inflammatory bowel disease (IBD).
  
  
  METHODS
  Male Wistar rats (200-220 g) were divided into four groups (n = 6). Chemical induction of IBD was done by sensitizing with 300 µL of 20 g/L of DNCB (in acetone) onto the nape of rats for 14 days followed by intra-colonic instillation of 250 µL of DNCB (0.1% DNCB in 50% alcohol) solution on day 15. Rats in Group 1 (normal control) and Group 2 (DNCB control) were treated with vehicle. Rats in Group 3 were treated with DHZ (100 mg/kg, p.o.; 8 days) and Group 4 animals were treated with sulfasalazine (SS) (100 mg/kg, p.o.; 8 days). On 24(th) day, the rats were killed, colon removed and the macroscopic, biochemical, and histopathological evaluations were performed.
  
  
  RESULTS
  The levels of myeloperoxidase, thiobarbituric acid reactive substrate, and nitrite increased significantly (P < 0.05) in the DNCB group whereas reduced significantly in the DHZ and SS treated groups. Serum nitrite levels were found to be insignificant between the different groups. Interleukin-6, tumor necrosis factor-alpha level was significantly high in the DNCB group.
  
  
  CONCLUSIONS
  These findings show that DHZ can be a promising molecule for the treatment of IBD.
authors.affiliation: Department of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal - 576104, Karnataka, India.
authors.forename: Phani Krishna
authors.initials: PK
authors.lastname: Kondamudi
authors.affiliation: Department of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal - 576104, Karnataka, India.
authors.forename: Hemalatha
authors.initials: H
authors.lastname: Kovelamudi
authors.affiliation: Department of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal - 576104, Karnataka, India.
authors.forename: Pawan G
authors.initials: PG
authors.lastname: Nayak
authors.affiliation: Department of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal - 576104, Karnataka, India.
authors.forename: Mallikarjuna Chamallamudi
authors.initials: MC
authors.lastname: Rao
authors.affiliation: Department of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal - 576104, Karnataka, India.
authors.forename: Rekha Raghuveer
authors.initials: RR
authors.lastname: Shenoy
keywords: Dehydrozingerone; di-nitro chlorobenzene; interleukin-6; myeloperoxidase; thiobarbituric acid reactive substrate; tumor necrosis factor-alpha