title: Effect of gyromagnetic fields on human prostatic adenocarcinoma cells.
abstract: OBJECTIVE
  To investigate the biological effect of gyromagnetic fields (GMFs) on cell proliferation and apoptosis of human prostatic adenocarcinoma cells and explore the underlying mechanisms.
  
  
  METHODS
  PC-3 cells were grouped into normal control (NC) and GMF treatment groups. Cell proliferation was analyzed with kit-8 and Ki67 immunofluorescence staining, while cell apoptosis was analyzed with flow cytometry double staining of Annexin V-PE/7-AAD. The Akt and p38 MAPK/Caspase signaling pathways were analyzed by western blotting and immunofluorescence staining, and cell polarization was analyzed with PARD3.
  
  
  RESULTS
  Cell proliferation and activity of the Akt pathway were significantly decreased by the GMF, while cell apoptosis, activity of p38 MAPK, and PARD3-positive cell number were significantly increased in the GMF group compared to the NC group.
  
  
  CONCLUSIONS
  GMFs inhibit cell proliferation, induce apoptosis, and regulate tumor cell polarity conditions, potentially through down-regulating Akt, activating the p38 MAPK/Caspase pathway, and promoting PARD3 expression in PC-3 cells.
authors.affiliation: Andrology Center, Peking University First Hospital, Peking University, Beijing, People's Republic of China.
authors.forename: Hongen
authors.initials: H
authors.lastname: Lei
authors.affiliation: Andrology Center, Peking University First Hospital, Peking University, Beijing, People's Republic of China.
authors.forename: Yongde
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Andrology Center, Peking University First Hospital, Peking University, Beijing, People's Republic of China.
authors.forename: Ruili
authors.initials: R
authors.lastname: Guan
authors.affiliation: Department of Urology, General Hospital of Ningxia Medical University, Yinchuan, People's Republic of China.
authors.forename: Meng
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Urology, The First Affiliated Hospital of Soochow University, Soochow, People's Republic of China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Hui
authors.affiliation: Andrology Center, Peking University First Hospital, Peking University, Beijing, People's Republic of China.
authors.forename: Zhezhu
authors.initials: Z
authors.lastname: Gao
authors.affiliation: Andrology Center, Peking University First Hospital, Peking University, Beijing, People's Republic of China.
authors.forename: Bicheng
authors.initials: B
authors.lastname: Yang
authors.affiliation: Andrology Center, Peking University First Hospital, Peking University, Beijing, People's Republic of China.
authors.forename: Zhongcheng
authors.initials: Z
authors.lastname: Xin
keywords: PC-3 cells; apoptosis; gyromagnetic fields; prostate cancer