title: A Comprehensive Toxicological Safety Assessment of an Extract of Olea Europaea L. Leaves (Bonolive™).
abstract: A battery of toxicological studies was conducted to investigate the genotoxicity and repeated-dose oral toxicity of Bonolive™, a proprietary water-soluble extract of the leaves of the olive tree (Olea europaea L.), in accordance with internationally accepted protocols. There was no evidence of mutagenicity in a bacterial reverse mutation test and in an vitro mammalian chromosomal aberration test nor was any genotoxic activity observed in an in vivo mouse micronucleus test at concentrations up to the limit dose of 2000 mg/kg bw/d. Bonolive™ did not cause mortality or toxic effects in Crl:(WI)BR Wistar rats in a 90-day repeated-dose oral toxicity study at doses of 360, 600, and 1000 mg/kg bw/d. The no observed adverse effect level in the 90-day study was 1000 mg/kg bw/d for both male and female rats, the highest dose tested.
authors.affiliation: AIBMR Life Sciences, Inc, 4117 South Meridian, Puyallup, WA, USA amy@aibmr.com.
authors.forename: Amy E
authors.initials: AE
authors.lastname: Clewell
authors.affiliation: Toxi-Coop Toxicological Research Center, Budapest, Hungary.
authors.forename: Erzsébet
authors.initials: E
authors.lastname: Béres
authors.affiliation: Toxi-Coop Toxicological Research Center, Budapest, Hungary.
authors.forename: Adél
authors.initials: A
authors.lastname: Vértesi
authors.affiliation: Toxi-Coop Toxicological Research Center, Budapest, Hungary.
authors.forename: Róbert
authors.initials: R
authors.lastname: Glávits
authors.affiliation: Toxi-Coop Toxicological Research Center, Budapest, Hungary.
authors.forename: Gábor
authors.initials: G
authors.lastname: Hirka
authors.affiliation: AIBMR Life Sciences, Inc, 4117 South Meridian, Puyallup, WA, USA.
authors.forename: John R
authors.initials: JR
authors.lastname: Endres
authors.affiliation: AIBMR Life Sciences, Inc, 4117 South Meridian, Puyallup, WA, USA.
authors.forename: Timothy S
authors.initials: TS
authors.lastname: Murbach
authors.affiliation: Toxi-Coop Toxicological Research Center, Budapest, Hungary.
authors.forename: Ilona Pasics
authors.initials: IP
authors.lastname: Szakonyiné
mesh_terms: D000818:Animals; D002869:Chromosome Aberrations; D004305:Dose-Response Relationship, Drug; D005260:Female; D008297:Male; D051379:Mice; D009152:Mutagenicity Tests; D019055:No-Observed-Adverse-Effect Level; D031658:Olea / Q000737:chemistry*; D010936:Plant Extracts / Q000633:toxicity*; D018515:Plant Leaves / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D010936:Plant Extracts
keywords: Bonolive™; Olea europaea L. leaves; extract; safety assessment; toxicity