title: Long-term effects of methamphetamine exposure in adolescent mice on the future ovarian reserve in adulthood.
abstract: Currently, there is an increasing prevalence of adolescent exposure to methamphetamine (MA). However, there is a paucity of information concerning the long-term impact of early exposure to MA upon female fertility and ovarian reserve. The aim of this study was to investigate the effect of long-term MA exposure in adolescents on their ovarian reserve in adulthood. Adolescent mice received intraperitoneal injections of MA (5mg/kg, three times per week) or saline from the 21st postnatal day for an 8 week period. Morphological, histological, biochemical, hormonal and ethological parameters were evaluated. An impaired ovarian reserve and vitality was found in the group treated with MA, manifesting in morphological-apparent mitochondrial damage, an activated apoptosis pathway in the ovarian tissue, a downward expression of ovarian anti-Mullerian hormone (AMH), a decreased number of primordial and growing follicles, an increased number of atretic follicles, and a depressed secretion of AMH, estradiol and progesterone from granulosa cells. However, no significant difference was noticed regarding the estrous cycle, the mating ability and the fertility outcome in the reproductive age of the mice after a period of non-medication. The present results confirmed that a long term exposure to methamphetamine in adolescent mice does have an adverse impact on their ovarian reserve, which indicates that such an early abuse of MA might influence the fertility lifespan of the female mouse.
authors.affiliation: Reproductive Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.
authors.forename: Lan
authors.initials: L
authors.lastname: Wang
authors.affiliation: Criminal Science and Technology Institute of Public Security Bureau of Wuxi City, Wuxi, Jiangsu 214000, China.
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Qu
authors.affiliation: Reproductive Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.
authors.forename: Xiyuan
authors.initials: X
authors.lastname: Dong
authors.affiliation: Reproductive Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China; Reproductive Medicine Center, The First Affiliated Hospital of Zheng Zhou University, Zhengzhou, Henan 450000, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Huang
authors.affiliation: Laboratory of Reproductive Medicine, New York University Langone Medical Center, New York, NY 10014, USA.
authors.forename: Molly
authors.initials: M
authors.lastname: Kumar
authors.affiliation: Reproductive Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.
authors.forename: Licheng
authors.initials: L
authors.lastname: Ji
authors.affiliation: Reproductive Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.
authors.forename: Ya
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Reproductive Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.
authors.forename: Junning
authors.initials: J
authors.lastname: Yao
authors.affiliation: Reproductive Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.
authors.forename: Shulin
authors.initials: S
authors.lastname: Yang
authors.affiliation: Reproductive Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.
authors.forename: Ruxing
authors.initials: R
authors.lastname: Wu
authors.affiliation: Reproductive Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China. Electronic address: hwzhang@tjh.tjmu.edu.cn.
authors.forename: Hanwang
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000367:Age Factors; D019969:Amphetamine-Related Disorders / Q000150:complications*; D000818:Animals; D054304:Anti-Mullerian Hormone / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects; D002478:Cells, Cultured; D000697:Central Nervous System Stimulants / Q000633:toxicity*; D004958:Estradiol / Q000378:metabolism; D005260:Female; D007247:Infertility, Female / Q000209:etiology* / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D008694:Methamphetamine / Q000633:toxicity*; D008813:Mice, Inbred ICR; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D006080:Ovarian Follicle / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D065851:Ovarian Reserve / Q000187:drug effects*; D010053:Ovary / Q000187:drug effects* / Q000378:metabolism / Q000503:physiopathology / Q000648:ultrastructure; D011374:Progesterone / Q000378:metabolism; D018570:Risk Assessment; D012307:Risk Factors; D046468:Sexual Development; D013997:Time Factors
chemical_list: D000697:Central Nervous System Stimulants; D008694:Methamphetamine; D011374:Progesterone; D004958:Estradiol; D054304:Anti-Mullerian Hormone
keywords: Adolescent; Anti-Mullerian hormone; Methamphetamine; Ovarian follicle counting; Ovarian reserve