title: A maize death acid, 10-oxo-11-phytoenoic acid, is the predominant cyclopentenone signal present during multiple stress and developmental conditions.
abstract: Recently we investigated the function of the 9-lipoxygenase (LOX) derived cyclopentenones 10-oxo-11-phytoenoic acid (10-OPEA) and 10-oxo-11,15-phytodienoic acid (10-OPDA) and identified their C-14 and C-12 derivatives. 10-OPEA accumulation is elicited by fungal and insect attack and acts as a strong inhibitor of microbial and herbivore growth. Although structurally similar, comparative analyses between 10-OPEA and its 13-LOX analog 12-oxo-phytodienoic acid (12-OPDA) demonstrate specificity in transcript accumulation linked to detoxification, secondary metabolism, jasmonate regulation, and protease inhibition. As a potent cell death signal, 10-OPEA activates cysteine protease activity leading to ion leakage and apoptotic-like DNA fragmentation. In this study we further elucidate the distribution, abundance, and functional roles of 10-OPEA, 10-OPDA, and 12-OPDA, in diverse organs under pathogen- and insect-related stress.
authors.affiliation: a Chemistry Research Unit, Center for Medical, Agricultural, and Veterinary Entomology, US Department of Agriculture-Agricultural Research Service (USDA-ARS) , Gainesville , FL , USA.
authors.forename: Shawn A
authors.initials: SA
authors.lastname: Christensen
authors.affiliation: b Section of Cell and Developmental Biology, University of California at San Diego , La Jolla , CA , USA.
authors.forename: Alisa
authors.initials: A
authors.lastname: Huffaker
authors.affiliation: a Chemistry Research Unit, Center for Medical, Agricultural, and Veterinary Entomology, US Department of Agriculture-Agricultural Research Service (USDA-ARS) , Gainesville , FL , USA.
authors.forename: Charles T
authors.initials: CT
authors.lastname: Hunter
authors.affiliation: a Chemistry Research Unit, Center for Medical, Agricultural, and Veterinary Entomology, US Department of Agriculture-Agricultural Research Service (USDA-ARS) , Gainesville , FL , USA.
authors.forename: Hans T
authors.initials: HT
authors.lastname: Alborn
authors.affiliation: b Section of Cell and Developmental Biology, University of California at San Diego , La Jolla , CA , USA.
authors.forename: Eric A
authors.initials: EA
authors.lastname: Schmelz
mesh_terms: D053898:Biosynthetic Pathways; D003517:Cyclopentanes / Q000737:chemistry / Q000378:metabolism*; D060434:Herbivory; D058945:Lipoxygenases / Q000737:chemistry / Q000378:metabolism; D008954:Models, Biological; D015398:Signal Transduction; D013312:Stress, Physiological; D003313:Zea mays / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*
chemical_list: D003517:Cyclopentanes; D058945:Lipoxygenases; C013905:cyclopentenone
keywords: 12-oxophytodienoic acid; 9-lipoxygenase; Cyclopentenone; Ten-oxo-11-phytoenoic acid; defense; maize; oxylipin