title: [Chemical Components from Leaves of Fatsia japonica and Their Antitumor Activities in vitro].
abstract: OBJECTIVE
  To study the chemical components from the leaves of Fatsia japonica and their antitumor activities in vitro.
  
  
  METHODS
  All compounds were separated and purified by column chromatography over silica gel, Sephadex LH-20 and preparative HPLC. Their structures were identified by physical and chemical properties and spectral methods including 1H-NMR and 13C-NMR. Antitumor assay was measured by MTT method.
  
  
  RESULTS
  18 compounds were isolated and identified as palmitic acid (1), β-hydroxypropiovanillone (2), adenosine (3), β-sitosterol (4), daucosterol (5), oleanolic acid (6), echinocystic acid (7), betulinic acid (8), hederagenin(9), hederagenin-3-O-α-L-rhamnopyranosyl(1-->2)-α-L-arabinopyranoside(10), acacetin(11), quercetin(12), quercetin-3-O-β-D-glucopyranoside(13), isovitexin(14), isovitexin-7-O-glucoside(15), astragalin(16), methylpluviatolide(17), and syringaresinol-4-O-β-D-glucopyranoside(18).
  
  
  CONCLUSIONS
  All compounds are isolated from the leaves of Fatsia japonica for the first time except compound 1. The ethyl acetate extract and compounds 6, 10, 12 and 18 at the concentration of 0. 5 mg/mL showed inhibitory effect against the proliferation of colon cell line A549 with the inhibitory rate over 90% in vitro.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wei
authors.forename: Zhen
authors.initials: Z
authors.lastname: Qiu
authors.forename: Fei
authors.initials: F
authors.lastname: Xu
authors.forename: Qian-rong
authors.initials: QR
authors.lastname: Li
authors.forename: Hao
authors.initials: H
authors.lastname: Yin
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry* / Q000302:isolation & purification; D027481:Araliaceae / Q000737:chemistry*; D045744:Cell Line, Tumor; D002851:Chromatography, High Pressure Liquid; D047309:Flavones; D005663:Furans; D005960:Glucosides; D006801:Humans; D044949:Kaempferols; D007783:Lactones; D017705:Lignans; D009828:Oleanolic Acid / Q000031:analogs & derivatives; D053978:Pentacyclic Triterpenes; D064209:Phytochemicals / Q000737:chemistry* / Q000302:isolation & purification; D018515:Plant Leaves / Q000737:chemistry*; D012855:Sitosterols; D014315:Triterpenes; D000094062:Betulinic Acid
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D047309:Flavones; D005663:Furans; D005960:Glucosides; D044949:Kaempferols; D007783:Lactones; D017705:Lignans; D053978:Pentacyclic Triterpenes; D064209:Phytochemicals; D012855:Sitosterols; D014315:Triterpenes; C508932:methylpluviatolide; C407309:syringaresinol-4''-O-glucoside; C042192:syringaresinol; C025473:gamma-sitosterol; D009828:Oleanolic Acid; C001579:astragalin; C023717:acacetin; C022999:echinocystic acid; C025763:hederagenin; D000094062:Betulinic Acid