title: NCBI-compliant genome submissions: tips and tricks to save time and money.
abstract: Genome sequences nowadays play a central role in molecular biology and bioinformatics. These sequences are shared with the scientific community through sequence databases. The sequence repositories of the International Nucleotide Sequence Database Collaboration (INSDC, comprising GenBank, ENA and DDBJ) are the largest in the world. Preparing an annotated sequence in such a way that it will be accepted by the database is challenging because many validation criteria apply. In our opinion, it is an undesirable situation that researchers who want to submit their sequence need either a lot of experience or help from partners to get the job done. To save valuable time and money, we list a number of recommendations for people who want to submit an annotated genome to a sequence database, as well as for tool developers, who could help to ease the process.
authors.affiliation: BaseClear B,V,, Genome analysis and technology department, Einsteinweg 5, Leiden 2333 CC, The Netherlands.
authors.forename: Walter
authors.initials: W
authors.lastname: Pirovano
authors.affiliation: BaseClear B.V., Einsteinweg 5, 2333 CC Leiden, Leiden,The Netherlands.
authors.forename: Marten
authors.initials: M
authors.lastname: Boetzer
authors.affiliation: Bioinformatics Group, Wageningen University, Wageningen, The Netherlands.
authors.forename: Martijn F L
authors.initials: MF
authors.lastname: Derks
authors.affiliation: Bioinformatics Group, Wageningen University, Wageningen, The Netherlands.
authors.forename: Sandra
authors.initials: S
authors.lastname: Smit
mesh_terms: D030561:Databases, Nucleic Acid; D016678:Genome; D006801:Humans; D020407:Internet; D009317:National Library of Medicine (U.S.); D014481:United States
keywords: GenBank; annotation; database submission; genome