title: Computational based functional analysis of Bacillus phytases.
abstract: Phytase is an enzyme which catalyzes the total hydrolysis of phytate to less phosphorylated myo-inositol derivatives and inorganic phosphate and digests the undigestable phytate part present in seeds and grains and therefore provides digestible phosphorus, calcium and other mineral nutrients. Phytases are frequently added to the feed of monogastric animals so that bioavailability of phytic acid-bound phosphate increases, ultimately enhancing the nutritional value of diets. The Bacillus phytase is very suitable to be used in animal feed because of its optimum pH with excellent thermal stability. Present study is aimed to perform an in silico comparative characterization and functional analysis of phytases from Bacillus amyloliquefaciens to explore physico-chemical properties using various bio-computational tools. All proteins are acidic and thermostable and can be used as suitable candidates in the feed industry.
authors.affiliation: Department of Biotechnology, Jaypee Institute of Information Technology (Deemed University), A-10, Sector-62, Noida, U.P., India.
authors.forename: Anukriti
authors.initials: A
authors.lastname: Verma
authors.affiliation: Centre for Bioinformatics, School of Biotechnology, Banaras Hindu University, Varanasi, India.
authors.forename: Vinay Kumar
authors.initials: VK
authors.lastname: Singh
authors.affiliation: Department of Biotechnology, Jaypee Institute of Information Technology (Deemed University), A-10, Sector-62, Noida, U.P., India. Electronic address: smriti.gaur@jiit.ac.in.
authors.forename: Smriti
authors.initials: S
authors.lastname: Gaur
mesh_terms: D010832:6-Phytase / Q000737:chemistry*; D001407:Bacillus / Q000201:enzymology*; D001426:Bacterial Proteins / Q000737:chemistry*; D003198:Computer Simulation; D007526:Isoelectric Point; D000072417:Protein Domains; D017433:Protein Structure, Secondary; D017386:Sequence Homology, Amino Acid
chemical_list: D001426:Bacterial Proteins; D010832:6-Phytase
keywords: Bacillus species; In silico characterization; Industrial applications; Phytases