title: Designed synthesis of carbon-functional magnetic graphene mesoporous silica materials using polydopamine as carbon precursor for the selective enrichment of N-linked glycan.
abstract: Glycosylation, which has been confirmed to be associated with many diseases, is an important protein post-translation modification. Taking into account the low abundant of glycan, the purification of complex biological samples is considered to be very significant before mass spectrometry detection. In this work, carbon-functionalized magnetic graphene /mesoporous silica materials (C-Mag G@mSiO2 materials) with high content of carbon were designed and synthesized by using polydopamine as carbon precursor. Taking advantage of the special interaction between carbon and glycan, C-Mag G@mSiO2 materials were successfully applied to enrich N-linked glycans in different complex samples, such as standard glycoprotein digestion, the mixture of standard glycoprotein digestion, glycoprotein and non-glycoprotein, and human serum.
authors.affiliation: Department of Chemistry and Institutes of Biomedical Sciences, Collaborative Innovation Center of Genetics and Development, Fudan University, Shanghai 200433, China.
authors.forename: Nianrong
authors.initials: N
authors.lastname: Sun
authors.affiliation: Department of Chemistry and Institutes of Biomedical Sciences, Collaborative Innovation Center of Genetics and Development, Fudan University, Shanghai 200433, China.
authors.forename: Jizong
authors.initials: J
authors.lastname: Yao
authors.affiliation: Department of Chemistry and Institutes of Biomedical Sciences, Collaborative Innovation Center of Genetics and Development, Fudan University, Shanghai 200433, China. Electronic address: chdeng@fudan.edu.cn.
authors.forename: Chunhui
authors.initials: C
authors.lastname: Deng
mesh_terms: D000818:Animals; D002244:Carbon / Q000737:chemistry*; D002417:Cattle; D002645:Chickens; D006108:Graphite / Q000138:chemical synthesis*; D006801:Humans; D007211:Indoles / Q000138:chemical synthesis*; D060328:Magnetic Phenomena; D011108:Polymers / Q000138:chemical synthesis*; D011134:Polysaccharides / Q000138:chemical synthesis*; D012822:Silicon Dioxide / Q000138:chemical synthesis*
chemical_list: D007211:Indoles; D011108:Polymers; D011134:Polysaccharides; C568283:polydopamine; D002244:Carbon; D012822:Silicon Dioxide; D006108:Graphite
keywords: Graphene; Mass spectrometry; N-linked glycan; Polydopamine; Selective enrichment