title: Chemical Composition and Characteristic Odor Compounds in Essential Oil from Alismatis Rhizoma (Tubers of Alisma orientale).
abstract: Chemical composition and potent odorants that contribute to the characteristic odor of essential oil from Alismatis Rhizoma (tubers of Alisma orientale) were investigated by gas chromatography-mass spectrometry (GC-MS), GC-olfactometry (GC-O), aroma extract dilution analysis (AEDA) and relative flavor activity (RFA) methods. Fifty components, representing 94.5% of the total oil, were identified. In this study, we newly identified thirty-nine compounds in the oil from tubers of A. orientale. The major constituents of the essential oil were khusinol (36.2%), δ-elemene (12.4%), germacron (4.1%), alismol (3.8%), β-elemene (3.1%), and α-bisabolol (1.9%). Through sensory analysis, sixteen aroma-active compounds were detected and the key contributing aroma-active compounds were δ-elemene (woody, flavor dilution (FD)-factor = 4, RFA = 0.3) β-elemene (spicy, FD = 5, RFA = 0.7), spathulenol (green, FD = 5, RFA = 1.0), γ-eudesmol (woody, FD = 6, RFA = 1.5), and γ-cadinol (woody, FD = 5, RFA = 1.0). These compounds are thought to contribute to the odor from tubers of A. orientale. These results imply that the essential oil from the tubers of A. orientale deserve further investigations in the phytochemical and medicinal fields.
authors.affiliation: Department of Applied Chemistry, Faculty of Science and Engineering, Kinki University (Kindai University).
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Miyazawa
authors.forename: Seiji
authors.initials: S
authors.lastname: Yoshinaga
authors.forename: Yusei
authors.initials: Y
authors.lastname: Kashima
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nakahashi
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Hara
authors.forename: Hiroki
authors.initials: H
authors.lastname: Nakagawa
authors.forename: Atsushi
authors.initials: A
authors.lastname: Usami
mesh_terms: D034902:Alisma / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D007201:Indicator Dilution Techniques; D000081222:Monocyclic Sesquiterpenes; D009812:Odorants / Q000032:analysis*; D009822:Oils, Volatile / Q000737:chemistry* / Q000302:isolation & purification*; D064367:Olfactometry; D018547:Plant Stems / Q000737:chemistry*; D012717:Sesquiterpenes / Q000032:analysis / Q000302:isolation & purification; D045788:Sesquiterpenes, Germacrane / Q000032:analysis / Q000302:isolation & purification
chemical_list: D000081222:Monocyclic Sesquiterpenes; D009822:Oils, Volatile; D012717:Sesquiterpenes; D045788:Sesquiterpenes, Germacrane; C573651:khusiol; C038905:elemene; C004497:bisabolol; C048393:germacrone; C013258:spathulenol; C053720:alismol