title: The Lipidomic Analyses in Low and Highly Aggressive Ovarian Cancer Cell Lines.
abstract: Despite huge advances in the research of epithelial ovarian cancer (EOC), it remains the most lethal gynecological malignancy. Peritoneal tumor cell dissemination with cell survival and drug-resistance to taxane and platinum-based chemotherapy are two of the major challenges of EOC treatment. We have generated highly aggressive EOC cell lines (ID8-P1 lines or P1) from ID8-P0 (without in vivo passage, or P0) through in vivo passage in mice. We conducted lipidomic analyses in cells from ID8-P0 versus three ID8-P1 cell lines using ultra-high-performance liquid chromatography coupled to electrospray ionization tandem mass spectrometry. A total of 16 classes of lipids (149 individual lipids) were analyzed and compared between P0 and P1 cells. In addition to overall lipid profiles in EOC cells, we had several novel observations. Several classes and species of lipids have been identified to be differentially present in P0 versus P1 cells, which are potentially involved in the acquired aggressiveness of P1 cells. Triacylglycerols (TAG) were dramatically increased under detachment stress in EOC cells. Since survival of EOC cells under detachment is one of the major obstacles for EOC treatment, further studies identifying the molecular mechanisms controlling TAG increase may lead to new treatment modalities for EOC.
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry Chinese Academy of Sciences, Beijing, 100190, China. zhenwenzhao@iccas.ac.cn.
authors.forename: Zhenwen
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: Department of Obstetrics and Gynecology, Indiana University School of Medicine, 975 W. Walnut St. IB355A, Indianapolis, IN, 46202, USA.
authors.forename: Qingchun
authors.initials: Q
authors.lastname: Cai
authors.affiliation: Department of Obstetrics and Gynecology, Indiana University School of Medicine, 975 W. Walnut St. IB355A, Indianapolis, IN, 46202, USA. xu2@iupui.edu.
authors.forename: Yan
authors.initials: Y
authors.lastname: Xu
mesh_terms: D000818:Animals; D000077216:Carcinoma, Ovarian Epithelial; D045744:Cell Line, Tumor; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D008055:Lipids / Q000096:biosynthesis* / Q000145:classification / Q000235:genetics; D051379:Mice; D009367:Neoplasm Staging; D009375:Neoplasms, Glandular and Epithelial / Q000188:drug therapy / Q000235:genetics* / Q000473:pathology; D010051:Ovarian Neoplasms / Q000188:drug therapy / Q000235:genetics* / Q000473:pathology; D021241:Spectrometry, Mass, Electrospray Ionization; D014280:Triglycerides / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D008055:Lipids; D014280:Triglycerides
keywords: Electrospray ionization-mass spectrometry (ESI-MS); Epithelial ovarian cancer (EOC); ID8; Lipidomic analyses; Triacylglycerol (TAG)