title: Transcriptome analysis of the key role of GAT2 gene in the hyper-accumulation of copper in the oyster Crassostrea angulata.
abstract: One paradigm of oysters as the hyper-accumulators of many toxic metals is the inter-individual variation of metals, but the molecular mechanisms remain very elusive. A comprehensive analysis of the transcriptome of Crassostrea angulata was conducted to reveal the relationship between gene expression and differential Cu body burden in oysters. Gene ontology analysis for the differentially expressed genes showed that the neurotransmitter transporter might affect the oyster behavior, which in turn led to difference in Cu accumulation. The ATP-binding cassette transporters superfamily played an important role in the maintenance of cell Cu homeostasis, vitellogenin and apolipophorin transport, and elimination of excess Cu. Gill and mantle Cu concentrations were significantly reduced after silencing the GABA transporter 2 (GAT2) gene, but increased after the injection of GABA receptor antagonists, suggesting that the function of GABA transporter 2 gene was strongly related to Cu accumulation. These findings demonstrated that GABA transporter can control the action of transmitter GABA in the nervous system, thereby affecting the Cu accumulation in the gills and mantles.
authors.affiliation: State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361102, PR China.|College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, PR China.
authors.forename: Bo
authors.initials: B
authors.lastname: Shi
authors.affiliation: State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361102, PR China.|College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, PR China.
authors.forename: Zekun
authors.initials: Z
authors.lastname: Huang
authors.affiliation: School of Life Sciences, Xiamen University, Xiamen 361102, PR China.
authors.forename: Xu
authors.initials: X
authors.lastname: Xiang
authors.affiliation: State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361102, PR China.|College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, PR China.
authors.forename: Miaoqin
authors.initials: M
authors.lastname: Huang
authors.affiliation: Division of Life Science, The Hong Kong University of Science and Technology, Hong Kong, PR China.
authors.forename: Wen-Xiong
authors.initials: WX
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361102, PR China.|College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, PR China.
authors.forename: Caihuan
authors.initials: C
authors.lastname: Ke
mesh_terms: D000818:Animals; D003300:Copper / Q000378:metabolism*; D049896:Crassostrea / Q000235:genetics* / Q000378:metabolism*; D050485:GABA Plasma Membrane Transport Proteins / Q000235:genetics*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D059014:High-Throughput Nucleotide Sequencing; D009928:Organ Specificity / Q000235:genetics; D018079:Receptors, GABA / Q000378:metabolism; D015203:Reproducibility of Results; D059467:Transcriptome; D005680:gamma-Aminobutyric Acid / Q000378:metabolism / Q000494:pharmacology
chemical_list: D050485:GABA Plasma Membrane Transport Proteins; D018079:Receptors, GABA; D005680:gamma-Aminobutyric Acid; D003300:Copper