title: Human papillomavirus types 16 E1 mRNA is transcribed from P14 early promoter in cervical neoplasms.
abstract: High-risk human papillomavirus (HR-HPV) persistent infection is responsible for the development of the majority of cervical cancers. The therapy against HPV-associated cancer requires knowledge of the viral gene expression mechanisms. In this study, the polyadenylated polycistronic transcripts containing full-size E1ORF and produced from the early P14 promoter were detected for the first time in cervical tumors with episomal forms of the HPV16 genome. P14-initiated mRNAs were revealed also in precancerous lesions. The amount of P14-initiated transcripts was significantly less compared to transcripts initiated from the major P97 HPV16 promoter in cervical intraepithelial neoplasms and squamous cell carcinomas. The ratios of P97/P14-transcripts determined by qRT-PCR were unique for each clinical sample and varied in quite wide ranges independent of disease progression stages or tumor grade. These data suggest that the levels of P14- and P97-transcripts are regulated independently from each other in cervical neoplasms.
authors.affiliation: Laboratory of Molecular Biology of viruses, NN Blokhin Russian Cancer Research Center, Kashirskoe shosse 24, Moscow 115478, Russia. Electronic address: Fedorova-fedorova_maria@inbox.ru.
authors.forename: Maria
authors.initials: M
authors.lastname: Fedorova
authors.affiliation: Laboratory of Molecular Biology of viruses, NN Blokhin Russian Cancer Research Center, Kashirskoe shosse 24, Moscow 115478, Russia. Electronic address: Vinokurova-vinokourova@mail.ru.
authors.forename: Svetlana
authors.initials: S
authors.lastname: Vinokurova
authors.affiliation: Laboratory of Molecular Biology of viruses, NN Blokhin Russian Cancer Research Center, Kashirskoe shosse 24, Moscow 115478, Russia.
authors.forename: Larissa
authors.initials: L
authors.lastname: Pavlova
authors.affiliation: Laboratory of cell and virus oncogenes regulation, NN Blokhin Russian Cancer Research Center, Kashirskoe shosse 24, Moscow 115478, Russia.
authors.forename: Andrei
authors.initials: A
authors.lastname: Komel'kov
authors.affiliation: Scientific Consultative Department, NN Blokhin Russian Cancer Research Center, Kashirskoe shosse 24, Moscow 115478, Russia.
authors.forename: Ljubov
authors.initials: L
authors.lastname: Korolenkova
authors.affiliation: Laboratory of Molecular Biology of viruses, NN Blokhin Russian Cancer Research Center, Kashirskoe shosse 24, Moscow 115478, Russia. Electronic address: Kisseljov-flkhpv@yandex.ru.
authors.forename: Fjodor
authors.initials: F
authors.lastname: Kisseljov
authors.affiliation: Laboratory of Molecular Biology of viruses, NN Blokhin Russian Cancer Research Center, Kashirskoe shosse 24, Moscow 115478, Russia. Electronic address: natalia-kis@yndex.ru.
authors.forename: Natalia
authors.initials: N
authors.lastname: Kisseljova
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000821:virology; D005260:Female; D052162:Human papillomavirus 16 / Q000235:genetics*; D006801:Humans; D009856:Oncogene Proteins, Viral / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000032:analysis* / Q000096:biosynthesis; D012367:RNA, Viral / Q000032:analysis* / Q000096:biosynthesis; D060888:Real-Time Polymerase Chain Reaction; D014158:Transcription, Genetic; D002583:Uterine Cervical Neoplasms / Q000821:virology*; D002578:Uterine Cervical Dysplasia / Q000821:virology
chemical_list: C093478:E1 protein, Human papillomavirus 16; D009856:Oncogene Proteins, Viral; D012333:RNA, Messenger; D012367:RNA, Viral
keywords: HPV16 E1 transcripts; Human papillomavirus; P14 early promoter; cervical neoplasia