title: Hypocholesterolaemic mechanism of bitter melon aqueous extracts via inhibition of pancreatic cholesterol esterase and reduction of cholesterol micellar solubility.
abstract: This study investigated the hypocholesterolaemic effects of bitter melon aqueous extracts (BMAE) in vitro, the inhibitory effects of BMAE on pancreatic cholesterol esterase (CEase) and incorporation of cholesterol into micelles were investigated. BMAE decreased the in vitro micellar solubility of cholesterol in a dose-dependent manner. The conformation of CEase was investigated by means of circular dichroism (CD) and fluorescence. The result revealed the decrease of α-helix contents, increase of β-sheet and exposure of aromatic amino acid residuals. The incorporation of cholesterol into micelles was inhibited by BMAE. A complex was observed by transmission electron microscopy (TEM), which indicated interaction between cholesterol and BMAE. The result revealed that BMAE can play a role in decreased intestinal cholesterol absorption via inhibition of CEase, and of micelle formation.
authors.affiliation: a State Key Laboratory of Food Science and Technology , Jiangnan University , Wuxi , People's Republic of China ;|b National Engineering Research Center for Functional Food , Jiangnan University , Wuxi , People's Republic of China ;|c School of Food Science and Technology , Jiangnan University , Wuxi , People's Republic of China ;
authors.forename: Jianhui
authors.initials: J
authors.lastname: Su
authors.affiliation: a State Key Laboratory of Food Science and Technology , Jiangnan University , Wuxi , People's Republic of China ;|b National Engineering Research Center for Functional Food , Jiangnan University , Wuxi , People's Republic of China ;|c School of Food Science and Technology , Jiangnan University , Wuxi , People's Republic of China ;
authors.forename: Hongxin
authors.initials: H
authors.lastname: Wang
authors.affiliation: a State Key Laboratory of Food Science and Technology , Jiangnan University , Wuxi , People's Republic of China ;|b National Engineering Research Center for Functional Food , Jiangnan University , Wuxi , People's Republic of China ;|c School of Food Science and Technology , Jiangnan University , Wuxi , People's Republic of China ;
authors.forename: Chaoyang
authors.initials: C
authors.lastname: Ma
authors.affiliation: a State Key Laboratory of Food Science and Technology , Jiangnan University , Wuxi , People's Republic of China ;|b National Engineering Research Center for Functional Food , Jiangnan University , Wuxi , People's Republic of China ;|c School of Food Science and Technology , Jiangnan University , Wuxi , People's Republic of China ;
authors.forename: Chengxiang
authors.initials: C
authors.lastname: Liu
authors.affiliation: d Anhui Tongling Ruipu Peony Industry Development Co, Ltd , Anhui , People's Republic of China.
authors.forename: Chuanzhong
authors.initials: C
authors.lastname: Gao
authors.affiliation: d Anhui Tongling Ruipu Peony Industry Development Co, Ltd , Anhui , People's Republic of China.
authors.forename: Rongjing
authors.initials: R
authors.lastname: Nie
authors.affiliation: a State Key Laboratory of Food Science and Technology , Jiangnan University , Wuxi , People's Republic of China ;|b National Engineering Research Center for Functional Food , Jiangnan University , Wuxi , People's Republic of China ;|c School of Food Science and Technology , Jiangnan University , Wuxi , People's Republic of China ;
authors.forename: Md Ramim
authors.initials: MR
authors.lastname: Tanver Rahman
mesh_terms: D000924:Anticholesteremic Agents / Q000494:pharmacology*; D002784:Cholesterol / Q000378:metabolism*; D007408:Intestinal Absorption / Q000187:drug effects; D008823:Micelles; D029777:Momordica charantia; D010179:Pancreas; D010936:Plant Extracts / Q000494:pharmacology*; D011487:Protein Conformation; D012995:Solubility; D002787:Sterol Esterase / Q000378:metabolism*
chemical_list: D000924:Anticholesteremic Agents; D008823:Micelles; D010936:Plant Extracts; D002784:Cholesterol; C054784:bile salt-stimulated lipase; D002787:Sterol Esterase
keywords: Bitter melon aqueous extracts; cholesterol micelles solubility; inhibition; pancreatic cholesterol esterase