title: Helicobacter pylori with high thioredoxin-1 expression promotes stomach carcinogenesis in Mongolian gerbils.
abstract: OBJECTIVE
  Previous studies by this group have shown that Helicobacter pylori with high thioredoxin-1 (Trx1) expression might be involved in stomach carcinogenesis in vitro. To study histopathological changes of the stomach mucosa in vivo, a Mongolian gerbil model infected with H. pylori with high Trx1 expression was established.
  
  
  METHODS
  Healthy, male Mongolian gerbils (n=75) were randomly divided into 3 groups: controls (n=15), which were not infected with H. pylori, high Trx1 (n=30) which were infected with H. pylori with high Trx1 expression and low Trx1 (n=30) which were infected with low Trx1 expression H. pylori. The animals were sacrificed at 4, 20, 34, 48, 70 and 90 weeks after inoculation.
  
  
  RESULTS
  The Mongolian gerbil model of H. pylori infection was successfully established. Three animals died during the study, leaving 72 animals (controls, n=14; low Trx1, n=29; high Trx1, n=29) examined on schedule. Histopathological analysis of the stomach mucosa showed gradually increased aggravation over time in the high and low Trx1 groups. Compared with control and low Trx1, the histopathological changes were more serious in the high Trx1 group. At 90 weeks, no abnormal changes were found in the controls, but 62.5% of the high Trx1 group and 33.3% of the low Trx1 showed adenocarcinomas. The H. pylori Trx1 level in gastric cancer tissue was significantly higher than that from gastritis tissue. Within gastric cancer cells, high Trx1 expression in H. pylori significantly upregulated cyclin D1.
  
  
  CONCLUSIONS
  High Trx1 expression in H. pylori promoted stomach carcinogenesis. More studies are needed to confirm this finding.
authors.affiliation: Department of Gastroenterology, Peking University Shougang Hospital, Beijing 100041, PR China.
authors.forename: Lin-Na
authors.initials: LN
authors.lastname: Liu
authors.affiliation: Department of Gastroenterology, Peking University Third Hospital, Beijing 100191, PR China. Electronic address: dingshigang222@163.com.
authors.forename: Shi-Gang
authors.initials: SG
authors.lastname: Ding
authors.affiliation: Department of Gastroenterology, Peking University Third Hospital, Beijing 100191, PR China.
authors.forename: Yan-Yan
authors.initials: YY
authors.lastname: Shi
authors.affiliation: Department of Gastroenterology, Peking University Third Hospital, Beijing 100191, PR China.
authors.forename: He-Jun
authors.initials: HJ
authors.lastname: Zhang
authors.affiliation: Department of Gastroenterology, Peking University Third Hospital, Beijing 100191, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Gastroenterology, Hebei United University Affiliated Hospital, Hebei 063000, PR China.
authors.forename: Chao
authors.initials: C
authors.lastname: Zhang
mesh_terms: D000230:Adenocarcinoma / Q000382:microbiology / Q000473:pathology; D000818:Animals; D063646:Carcinogenesis; D019938:Cyclin D1 / Q000378:metabolism; D004195:Disease Models, Animal; D005753:Gastric Mucosa / Q000382:microbiology* / Q000473:pathology; D005849:Gerbillinae; D016481:Helicobacter Infections / Q000382:microbiology*; D016480:Helicobacter pylori / Q000378:metabolism* / Q000472:pathogenicity*; D008297:Male; D013274:Stomach Neoplasms / Q000382:microbiology* / Q000473:pathology; D013879:Thioredoxins / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D019938:Cyclin D1; D013879:Thioredoxins