title: Green synthesis of silver nanoparticles for selective toxicity towards cancer cells.
abstract: Therapeutic applications of nanoparticles (NPs) are rapidly increasing for their utility in medicine, especially cancer therapy. The present study investigated the green synthesis of silver NPs (Ag NPs) of 10 nm size using Sargassum vulgare and its preferential ability to kill cancerous human myeloblastic leukemic cells HL60 and cervical cancer cells HeLa as compared with normal peripheral blood mononuclear cells. DNA fragmentation study and annexin V marker fluorescence-activated cell sorting (FACS) analysis revealed the Ag NP-induced cell death is through apoptosis. Transmission electron micrographs have showed the endocytosis of Ag NPs into the nucleus. Ag NPs inhibited the lipid peroxidation-induced reactive oxygen species generation, thus preventing the irradiation-related carcinogenesis. This study suggested that a mechanism underlying the toxicity of Ag NPs towards cancer cells is due to DNA damage and apoptosis. The authors' findings revealed the potential utility of as-prepared Ag NPs in the treatment of cancer as prophylactic agent with antioxidant property and chemotherapeutic agent for their selective toxicity to cancer cells.
authors.affiliation: Nanoscience Division, Centre for Ocean Research, Sathyabama University, Chennai 600119, India.
authors.forename: Kasivelu
authors.initials: K
authors.lastname: Govindaraju
authors.affiliation: Nanomaterials and System Lab, Department of Mechanical Engineering, Jeju National University, Jeju 690-456, Republic of Korea.
authors.forename: Karthikeyan
authors.initials: K
authors.lastname: Krishnamoorthy
authors.affiliation: Biosciences Research Centre, Department of Medical Laboratory, College of Science, Majmaah University, Zulfi 11932, Saudi Arabia.
authors.forename: Suliman A
authors.initials: SA
authors.lastname: Alsagaby
authors.affiliation: Nanoscience Division, Department of Zoology, Thiruvalluvar University, Vellore 632004, Tamilnadu, India.
authors.forename: Ganesan
authors.initials: G
authors.lastname: Singaravelu
authors.affiliation: Biosciences Research Centre, Department of Medical Laboratory, College of Science, Majmaah University, Zulfi 11932, Saudi Arabia. m.premanathan@mu.edu.so.
authors.forename: Mariappan
authors.initials: M
authors.lastname: Premanathan
mesh_terms: D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000138:chemical synthesis; D002470:Cell Survival / Q000187:drug effects; D003460:Crystallization / Q000379:methods; D004058:Diffusion; D055772:Green Chemistry Technology / Q000379:methods*; D018922:HL-60 Cells; D006367:HeLa Cells; D006801:Humans; D053768:Metal Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry* / Q000648:ultrastructure; D009374:Neoplasms, Experimental / Q000737:chemistry / Q000188:drug therapy* / Q000473:pathology; D010316:Particle Size; D012834:Silver / Q000008:administration & dosage* / Q000737:chemistry*; D016896:Treatment Outcome
chemical_list: D000970:Antineoplastic Agents; D012834:Silver