title: An UPLC-MS/MS method for simultaneous quantitation of two coumarins and two flavonoids in rat plasma and its application to a pharmacokinetic study of Wikstroemia indica extract.
abstract: In this study, an ultra performance liquid chromatography tandem mass spectrometry method (UPLC-MS/MS) was developed for simultaneous determination of umbelliferone, apigenin, daphnoretin and genkwanin in total (free and conjugated) forms in rat plasma using psoralen as internal standard. Plasma samples were protein precipitated with acetonitrile followed by liquid-liquid extracted with ethyl acetate. Four ingredients were separated on an Acquity UPLC(®) BEH C18 column using gradient elution with the mobile phase consisting of 0.1% formic acid aqueous solution and acetonitrile, and detected by positive ion electrospray ionization (ESI) in multiple reaction monitoring mode (MRM). The method was linear for all analytes over investigated ranges with all correlation coefficients greater than 0.99. The validated lower limit of quantification was 3ng/mL for umbelliferone, 3ng/mL for apigenin, 12ng/mL for daphnoretin and 2ng/mL for genkwanin, respectively. Intra- and inter-day precisions (RSD%) were less than 15% and accuracy (RE%) ranged from -1.1% to 15%. The mean absolute extraction recoveries of analytes and IS from rat plasma were all more than 75%. The validated method was firstly and successfully applied to investigate the pharmacokinetics of four chemical ingredients after oral administration of Wikstroemia indica extract (WIE) to rats.
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.
authors.forename: Lan
authors.initials: L
authors.lastname: Wei
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Pharmacology, Shenyang Institute for Drug Control, Shenyang 110014, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhang
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.
authors.forename: Yangyang
authors.initials: Y
authors.lastname: Sun
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Jia
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.
authors.forename: Jingxin
authors.initials: J
authors.lastname: Zhao
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.
authors.forename: Shikai
authors.initials: S
authors.lastname: Dong
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China. Electronic address: sunlixin67@yahoo.com.
authors.forename: Lixin
authors.initials: L
authors.lastname: Sun
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D003374:Coumarins / Q000097:blood*; D005419:Flavonoids / Q000097:blood*; D010936:Plant Extracts / Q000032:analysis*; D051381:Rats; D053719:Tandem Mass Spectrometry / Q000379:methods*; D029645:Thymelaeaceae / Q000378:metabolism*
chemical_list: D003374:Coumarins; D005419:Flavonoids; D010936:Plant Extracts
keywords: Coumarin; Flavonoid; Pharmacokinetics; UPLC–MS/MS; Wikstroemia indica (L.) C. A. Mey