title: Inhibition of Helicobacter pylori and Its Associate Urease by Labdane Diterpenoids Isolated from Andrographis paniculata.
abstract: The present study was carried out to evaluate anti-Helicobacter pylori and its associated urease activity of labdane diterpenoids isolated from Andrographis paniculata. A molecular docking analysis was performed by using ArgusLab 4.0.1 software. The results obtained indicate that compound A possesses strong inhibition to H. pylori, 28 ± 2.98 (minimum inhibitory concentration, 9 µg/mL), and its urease, 85.54 ± 2.62% (IC50 , 20.2 µg/mL). Compounds B, C, and D also showed moderate inhibition to H. pylori and its urease. The obtained results were in agreement with the molecular docking analysis of compounds. The phytochemicals under investigation were found to be promising antibacterial agents. Moreover, the isolated compounds can be considered as a resource for searching novel anti-H. pylori agents possessing urease inhibition.
authors.affiliation: Department of Botany, Poona College, Camp, Pune, 411 001, MS, India.
authors.forename: Rafik U
authors.initials: RU
authors.lastname: Shaikh
authors.affiliation: School of Life Sciences, Swami Ramanand Teerth Marathwada University, Nanded, 431 606, MS, India.
authors.forename: Ashwini A
authors.initials: AA
authors.lastname: Dawane
authors.affiliation: Department of Chemistry, Deogiri College, Aurangabad, 431 005, MS, India.
authors.forename: Rajendra P
authors.initials: RP
authors.lastname: Pawar
authors.affiliation: Department of Microbiology, Savitribai Phule Pune University, Pune, 411 007, MS, India.
authors.forename: Dhananjay S
authors.initials: DS
authors.lastname: Gond
authors.affiliation: School of Life Sciences, Swami Ramanand Teerth Marathwada University, Nanded, 431 606, MS, India.
authors.forename: Rohan J
authors.initials: RJ
authors.lastname: Meshram
authors.affiliation: School of Life Sciences, Swami Ramanand Teerth Marathwada University, Nanded, 431 606, MS, India.
authors.forename: Rajesh N
authors.initials: RN
authors.lastname: Gacche
mesh_terms: D035685:Andrographis / Q000737:chemistry*; D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000037:antagonists & inhibitors; D004224:Diterpenes / Q000302:isolation & purification / Q000494:pharmacology*; D016480:Helicobacter pylori / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development; D008826:Microbial Sensitivity Tests; D062105:Molecular Docking Simulation; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D014510:Urease / Q000037:antagonists & inhibitors*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004224:Diterpenes; D010936:Plant Extracts; C030419:andrographolide; D014510:Urease
keywords: Andrographis paniculata; Helicobacter pylori; diterpenoids; urease