title: Down-regulation of SNX1 predicts poor prognosis and contributes to drug resistance in colorectal cancer.
abstract: As a potential tumor suppressor, the detailed clinical application value of sorting nexin 1 (SNX1) has not been elucidated in colorectal cancer (CRC). The aim of the present study was to evaluate the expression of SNX1 in CRC tissues and to determine its correlation with clinicopathologic characteristics and its impact on patient's prognosis. We detected the expression of SNX1 mRNA in 72 CRC patients and SNX1 protein in 237 CRC patients by real-time polymerase chain reaction (RT-PCR) and immunohistochemical staining, respectively. Relationship between the expression of SNX1 and various clinicopathological features in these patients was evaluated. Both the mRNA and protein expression of SNX1 were remarkably decreased in CRC tissues compared with paired non-cancerous tissues, and the down-regulation of SNX1 protein was strongly associated with poor differentiation and poor overall survival (OS) rate of CRC patients. Ectopic SNX1 expression repressed CRC cell growth and promoted tumor sensitivity to most commonly used chemotherapeutic drugs (oxaliplatin and 5-Fluorouracil). In conclusion, overexpression of SNX1 may serve as a new therapeutic strategy for CRC.
authors.affiliation: Wuxi Oncology Institute, The Affiliated Hospital of Jiangnan University, 200 Huihe Road, Wuxi, Jiangsu, 214062, China.
authors.forename: Zehua
authors.initials: Z
authors.lastname: Bian
authors.affiliation: Wuxi Oncology Institute, The Affiliated Hospital of Jiangnan University, 200 Huihe Road, Wuxi, Jiangsu, 214062, China.
authors.forename: Yuyang
authors.initials: Y
authors.lastname: Feng
authors.affiliation: Wuxi Oncology Institute, The Affiliated Hospital of Jiangnan University, 200 Huihe Road, Wuxi, Jiangsu, 214062, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Xue
authors.affiliation: Wuxi Oncology Institute, The Affiliated Hospital of Jiangnan University, 200 Huihe Road, Wuxi, Jiangsu, 214062, China.
authors.forename: Yaling
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Department of Pathology, Fudan University Shanghai Cancer Center, Shanghai, 200032, China.
authors.forename: Qifeng
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Wuxi Oncology Institute, The Affiliated Hospital of Jiangnan University, 200 Huihe Road, Wuxi, Jiangsu, 214062, China.
authors.forename: Leyuan
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Wuxi Oncology Institute, The Affiliated Hospital of Jiangnan University, 200 Huihe Road, Wuxi, Jiangsu, 214062, China.
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Wuxi Oncology Institute, The Affiliated Hospital of Jiangnan University, 200 Huihe Road, Wuxi, Jiangsu, 214062, China.
authors.forename: Jiwei
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Wuxi Oncology Institute, The Affiliated Hospital of Jiangnan University, 200 Huihe Road, Wuxi, Jiangsu, 214062, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yin
authors.affiliation: Department of Laboratory Medicine, Affiliated Hospital of Xuzhou Medical College, Xuzhou, Jiangsu, 221002, China.|Medical Technology Institute of Xuzhou Medical College, Xuzhou, Jiangsu, 221002, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Gu
authors.affiliation: Wuxi Oncology Institute, The Affiliated Hospital of Jiangnan University, 200 Huihe Road, Wuxi, Jiangsu, 214062, China. hzhwxsy@126.com.
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Huang
mesh_terms: D000328:Adult; D000368:Aged; D000970:Antineoplastic Agents / Q000494:pharmacology; D014408:Biomarkers, Tumor; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000401:mortality* / Q000473:pathology; D015536:Down-Regulation; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D005260:Female; D005472:Fluorouracil / Q000494:pharmacology; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053208:Kaplan-Meier Estimate; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D011379:Prognosis; D058305:Sorting Nexins / Q000235:genetics*; D047368:Tumor Burden
chemical_list: D000970:Antineoplastic Agents; D014408:Biomarkers, Tumor; C513978:SNX1 protein, human; D058305:Sorting Nexins; D005472:Fluorouracil
keywords: Colorectal cancer (CRC); Drug resistance; Prognosis; Sorting nexin 1 (SNX1)