title: Systematic evaluation of self-adjuvanting lipopeptide nano-vaccine platforms for the induction of potent CD8(+) T-cell responses.
abstract: OBJECTIVE
  Systematically evaluate lipid core peptide vaccine delivery platforms to identify core features promoting strong CD8(+) T-cell responses.
  
  
  METHODS
  Three different self-adjuvanting lipid core peptide nanovaccines each comprising four copies of the dominant ovalbumin CD8(+) T-cell epitope and varying in the utilization of a polylysine or glucose core with 2-amino-hexadecanoic acid (C16) or 2-amino-dodecanoic acid (C12) lipids were synthesized. Vaccines were tested for ability to induce CD8(+) T-cell responses and inhibit tumor growth in vivo.
  
  
  RESULTS
  The construct utilizing C12 lipids and polylysine core induced very robust effector T cells shown to have in vivo effector capability as demonstrated by in vivo cytotoxicity and ability to inhibit tumor growth as well as modulation of dendritic cell activation.
  
  
  CONCLUSIONS
  The C12 polylysine platform was an effective configuration for induction of potent CD8(+) T-cell responses.
authors.affiliation: Infectious Diseases Programme, QIMR Berghofer Medical Research Institute, Herston, QLD 4006, Australia.
authors.forename: Simon H
authors.initials: SH
authors.lastname: Apte
authors.affiliation: School of Chemistry & Molecular Biosciences, The University of Queensland, St Lucia, QLD 4072, Australia.
authors.forename: Rachel J
authors.initials: RJ
authors.lastname: Stephenson
authors.affiliation: School of Chemistry & Molecular Biosciences, The University of Queensland, St Lucia, QLD 4072, Australia.
authors.forename: Pavla
authors.initials: P
authors.lastname: Simerska
authors.affiliation: Infectious Diseases Programme, QIMR Berghofer Medical Research Institute, Herston, QLD 4006, Australia.
authors.forename: Penny L
authors.initials: PL
authors.lastname: Groves
authors.affiliation: School of Chemistry & Molecular Biosciences, The University of Queensland, St Lucia, QLD 4072, Australia.
authors.forename: Salwa
authors.initials: S
authors.lastname: Aljohani
authors.affiliation: School of Chemistry & Molecular Biosciences, The University of Queensland, St Lucia, QLD 4072, Australia.
authors.forename: Sharareh
authors.initials: S
authors.lastname: Eskandari
authors.affiliation: School of Chemistry & Molecular Biosciences, The University of Queensland, St Lucia, QLD 4072, Australia.|School of Pharmacy, The University of Queensland, Woolloongabba, QLD 4012, Australia.|Institute for Molecular Bioscience, The University of Queensland, St Lucia, QLD 4072, Australia.
authors.forename: Istvan
authors.initials: I
authors.lastname: Toth
authors.affiliation: Infectious Diseases Programme, QIMR Berghofer Medical Research Institute, Herston, QLD 4006, Australia.
authors.forename: Denise L
authors.initials: DL
authors.lastname: Doolan
mesh_terms: D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D019496:Cancer Vaccines / Q000008:administration & dosage* / Q000737:chemistry; D007850:Lauric Acids / Q000737:chemistry*; D055666:Lipopeptides / Q000008:administration & dosage* / Q000737:chemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D053769:Nanocapsules / Q000737:chemistry* / Q000648:ultrastructure; D009374:Neoplasms, Experimental / Q000276:immunology / Q000628:therapy*; D010316:Particle Size; D011107:Polylysine / Q000737:chemistry; D016896:Treatment Outcome
chemical_list: D019496:Cancer Vaccines; D007850:Lauric Acids; D055666:Lipopeptides; D053769:Nanocapsules; D011107:Polylysine; C046049:12-aminododecanoic acid
keywords: CD8+ T cells; dendritic cells; lipid core peptide; nanovaccine; self-adjuvant; vaccine