title: Functional characteristics and clinical effectiveness of platelet concentrates treated with riboflavin and ultraviolet light in plasma and in platelet additive solution.
abstract: OBJECTIVE
  Pathogen reduction technologies may affect platelet quality during storage. We studied functional characteristics and clinical effectiveness of platelet concentrates (PCs) treated with Mirasol in plasma and in platelet-additive solution SSP+.
  
  
  METHODS
  Mirasol-treated, gamma-irradiated and untreated apheresis PCs were examined on days 0, 1, 3 and 5 of storage. Phosphatidylserine, P-selectin and active glycoprotein IIb/IIIa were analysed using flow cytometry before and after platelet stimulation. Platelet count increments, the numbers of inefficient transfusions and post-transfusion reactions were analysed to estimate clinical effectiveness.
  
  
  RESULTS
  A significant increase in all platelet activation markers occurred during storage in all PC groups. Activation markers in Mirasol-treated samples were already significantly higher compared with the control ones on the day of harvesting, and continued to grow during the storage. Mirasol treatment increased the number of platelets with a mitochondrial membrane potential loss. On the 3rd day of storage, 50% of Mirasol-treated platelets did not respond to activation; on the 5th day, none did. This agreed well with a decrease (approximately twofold) in the effectiveness of Mirasol-treated PC transfusions. Transfusions of PCs stored in SSP+ were accompanied by fewer inefficient transfusions and post-transfusion reactions than of PCs stored in plasma.
  
  
  CONCLUSIONS
  Treatment with Mirasol decreased platelet function, particularly profoundly on the 5th day of storage, and led to a decrease in the effectiveness of transfusions. SSP+ did not affect laboratory parameters significantly compared with plasma, but decreased the percentage of transfusion complications.
authors.affiliation: Federal Scientific Clinical Centre of Pediatric Hematology, Oncology and Immunology named after Dmitry Rogachev Ministry of Health of Russian, Moscow, Russian Federation.
authors.forename: A A
authors.initials: AA
authors.lastname: Ignatova
authors.affiliation: Federal Scientific Clinical Centre of Pediatric Hematology, Oncology and Immunology named after Dmitry Rogachev Ministry of Health of Russian, Moscow, Russian Federation.
authors.forename: O V
authors.initials: OV
authors.lastname: Karpova
authors.affiliation: Federal Scientific Clinical Centre of Pediatric Hematology, Oncology and Immunology named after Dmitry Rogachev Ministry of Health of Russian, Moscow, Russian Federation.
authors.forename: P E
authors.initials: PE
authors.lastname: Trakhtman
authors.affiliation: Federal Scientific Clinical Centre of Pediatric Hematology, Oncology and Immunology named after Dmitry Rogachev Ministry of Health of Russian, Moscow, Russian Federation.
authors.forename: S A
authors.initials: SA
authors.lastname: Rumiantsev
authors.affiliation: Federal Scientific Clinical Centre of Pediatric Hematology, Oncology and Immunology named after Dmitry Rogachev Ministry of Health of Russian, Moscow, Russian Federation.
authors.forename: M A
authors.initials: MA
authors.lastname: Panteleev
mesh_terms: D001792:Blood Platelets / Q000166:cytology* / Q000187:drug effects* / Q000528:radiation effects; D001793:Blood Preservation; D005434:Flow Cytometry; D006801:Humans; D053078:Membrane Potential, Mitochondrial; D019007:P-Selectin / Q000097:blood; D010718:Phosphatidylserines / Q000097:blood; D015539:Platelet Activation / Q000187:drug effects / Q000528:radiation effects; D010976:Platelet Count; D019039:Platelet Glycoprotein GPIIb-IIIa Complex / Q000032:analysis; D017713:Platelet Transfusion; D010983:Plateletpheresis; D012256:Riboflavin / Q000494:pharmacology*; D014466:Ultraviolet Rays
chemical_list: D019007:P-Selectin; D010718:Phosphatidylserines; D019039:Platelet Glycoprotein GPIIb-IIIa Complex; D012256:Riboflavin
keywords: SSP+; pathogen inactivation; platelet concentrates; platelet function; platelet transfusion