title: MicroRNA-9 Mediates the Cell Apoptosis by Targeting Bcl2l11 in Ischemic Stroke.
abstract: Ischemic strokes occur as a result of an obstruction within a blood vessel supplying blood to the brain and accounts for about 87 % of all cases. During the cerebral ischemia, most of the neurons undergo the necrosis and apoptosis upon the exposure to the dramatic blood flow reduction. Although, it is known that both the intrinsic and extrinsic pathways are involved in the neuronal apoptosis of ischemic brain injury. The complex underlying mechanisms remains less known. MicroRNAs are a class of endogenous small non-coding RNAs and the role of miRNAs in the pathophysiology of stroke has been studied. In this study, we found that miR-9 is downregulated in the mice with middle cerebral artery occlusion (MCAO) brain and oxygen-glucose deprivation (OGD) neurons. Application of miR-9 gamer could restore the neurological scores and reduces the infarct volume, brain water content, and the behavioral impairments. Moreover, upregulation of miR-9 suppresses the neuronal apoptosis in MCAO brain and OGD neurons. Furthermore, we identified that Bcl2l11 as the direct target of miR-9 and manipulation of miR-9 induces the corresponding changing of Bcl2l11 protein level. Finally, we found that the protein level of Bcl2l11 is increased in the MCAO brain and OGD neurons. Our study demonstrated the critical role of miR-9 in the neuronal apoptosis of ischemic brain injury.
authors.affiliation: Department of Pathology, the First Affiliated Hospital of Zhengzhou University, NO.1 Jian She Dong Avenue, Zhengzhou, 450002, People's Republic of China.
authors.forename: Na
authors.initials: N
authors.lastname: Wei
authors.affiliation: Department of Pathology, the First Affiliated Hospital of Zhengzhou University, NO.1 Jian She Dong Avenue, Zhengzhou, 450002, People's Republic of China.
authors.forename: Lin
authors.initials: L
authors.lastname: Xiao
authors.affiliation: Department of Urology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Xue
authors.affiliation: Department of Pathology, the First Affiliated Hospital of Zhengzhou University, NO.1 Jian She Dong Avenue, Zhengzhou, 450002, People's Republic of China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Department of Pathology, the First Affiliated Hospital of Zhengzhou University, NO.1 Jian She Dong Avenue, Zhengzhou, 450002, People's Republic of China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Department of Pathology, the First Affiliated Hospital of Zhengzhou University, NO.1 Jian She Dong Avenue, Zhengzhou, 450002, People's Republic of China.
authors.forename: Huayan
authors.initials: H
authors.lastname: Ren
authors.affiliation: Clinical Laboratory, Henan Provincial People's Hospital, 7 Wei Wu Road, Zhengzhou, 450000, China. siguo001@126.com.
authors.forename: Si
authors.initials: S
authors.lastname: Guo
authors.affiliation: Department of Pathology, the First Affiliated Hospital of Zhengzhou University, NO.1 Jian She Dong Avenue, Zhengzhou, 450002, People's Republic of China. xujingjing_1982@163.com.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Xu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D001483:Base Sequence; D000072224:Bcl-2-Like Protein 11 / Q000378:metabolism*; D001522:Behavior, Animal; D002545:Brain Ischemia / Q000150:complications / Q000235:genetics* / Q000473:pathology*; D004195:Disease Models, Animal; D005947:Glucose / Q000172:deficiency; D020244:Infarction, Middle Cerebral Artery / Q000150:complications / Q000473:pathology; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism / Q000473:pathology; D010100:Oxygen; D014176:Protein Biosynthesis; D020521:Stroke / Q000150:complications / Q000235:genetics* / Q000473:pathology*; D015854:Up-Regulation / Q000235:genetics
chemical_list: D000072224:Bcl-2-Like Protein 11; C000606776:Bcl2l11 protein, mouse; C551804:MIRN9 microRNA, mouse; D035683:MicroRNAs; D005947:Glucose; D010100:Oxygen
keywords: Apoptosis; Bcl2l11; Ischemic stroke; miR-9