title: ToF-SIMS multivariate analysis of surface-grafted small bioactive molecules.
abstract: In the development of bioactive coatings on biomaterials, it is essential to characterize the successful fabrication and the uniformity of intended coatings by sensitive surface analytical techniques, so as to ensure reliable interpretation of observed biointerfacial responses. This can, however, be challenging when small bioactive molecules are grafted onto biomaterials surfaces at sub- and near-monolayer densities. Time-of-flight secondary ion mass spectrometry (ToF-SIMS) provides the required sensitivity, but ion signals from small grafted molecules may still be dominated by fragment ions from the underlying polymer. In such cases, multivariate analysis provides valuable enhancement of spectral data, as illustrated here by examples comprising the surface grafting of bioactive serrulatane molecules, the peptide GRGDSP, the oligonucleotide 15-thymidine, and the antifungal compound Amphotericin B. The authors also show how ToF-SIMS plus principal component analysis can distinguish between covalent grafting and physisorption of the antibiotics caspofungin and micafungin.
authors.affiliation: Mawson Institute, University of South Australia, Mawson Lakes, South Australia 5095, Australia.
authors.forename: Marek
authors.initials: M
authors.lastname: Jasieniak
authors.affiliation: Mawson Institute, University of South Australia, Mawson Lakes, South Australia 5095, Australia.
authors.forename: Bryan R
authors.initials: BR
authors.lastname: Coad
authors.affiliation: Mawson Institute, University of South Australia, Mawson Lakes, South Australia 5095, Australia.
authors.forename: Hans J
authors.initials: HJ
authors.lastname: Griesser
mesh_terms: D001688:Biological Products / Q000032:analysis*; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D015999:Multivariate Analysis; D018629:Spectrometry, Mass, Secondary Ion; D013499:Surface Properties
chemical_list: D001688:Biological Products; D020099:Coated Materials, Biocompatible