title: Anomalous partitioning of water in coexisting liquid phases of lipid multilayers near 100% relative humidity.
abstract: Ternary lipid mixtures incorporating cholesterol are well-known to phase separate into liquid-ordered (L(o)) and liquid-disordered (L(d)) phases. In multilayers of these systems, the laterally phase separated domains register in columnar structures with different bilayer periodicities, resulting in hydrophobic mismatch energies at the domain boundaries. In this paper, we demonstrate via synchrotron-based X-ray diffraction measurements that the system relieves the hydrophobic mismatch at the domain boundaries by absorbing larger amounts of inter-bilayer water into the L(d) phase with lower d-spacing as the relative humidity approaches 100%. The lamellar repeat distance of the L(d) phase swells by an extra 4 Å, well beyond the equilibrium spacing predicted by the inter-bilayer forces. This anomalous swelling is caused by the hydrophobic mismatch energy at the domain boundaries, which produces a surprisingly long-range effect. We also demonstrate that the d-spacings of the lipid multilayers at 100% relative humidity do not change when bulk water begins to condense on the sample.
authors.affiliation: Department of Physics, University of California-San Diego, La Jolla, CA-92093, USA. ssinha@physics.ucsd.edu.
authors.forename: Yicong
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Department of Physics, University of California-San Diego, La Jolla, CA-92093, USA. ssinha@physics.ucsd.edu.
authors.forename: Sajal K
authors.initials: SK
authors.lastname: Ghosh
authors.affiliation: Department of Physics, University of California-San Diego, La Jolla, CA-92093, USA. ssinha@physics.ucsd.edu.
authors.forename: Sambhunath
authors.initials: S
authors.lastname: Bera
authors.affiliation: Advanced Photon Source, Argonne National Laboratory, Argonne, IL-60439, USA.
authors.forename: Zhang
authors.initials: Z
authors.lastname: Jiang
authors.affiliation: Advanced Photon Source, Argonne National Laboratory, Argonne, IL-60439, USA.
authors.forename: Christian M
authors.initials: CM
authors.lastname: Schlepütz
authors.affiliation: Advanced Photon Source, Argonne National Laboratory, Argonne, IL-60439, USA.
authors.forename: Evguenia
authors.initials: E
authors.lastname: Karapetrova
authors.affiliation: Department of Physics, Northern Illinois University, DeKalb, IL-60115, USA.
authors.forename: Laurence B
authors.initials: LB
authors.lastname: Lurio
authors.affiliation: Department of Physics, University of California-San Diego, La Jolla, CA-92093, USA. ssinha@physics.ucsd.edu.
authors.forename: Sunil K
authors.initials: SK
authors.lastname: Sinha
mesh_terms: D002784:Cholesterol / Q000737:chemistry*; D006813:Humidity; D057927:Hydrophobic and Hydrophilic Interactions; D008055:Lipids / Q000737:chemistry*; D010316:Particle Size; D013499:Surface Properties; D014867:Water / Q000737:chemistry*
chemical_list: D008055:Lipids; D014867:Water; D002784:Cholesterol