title: Increased Variability of Genomic Transcription in Schizophrenia.
abstract: Schizophrenia (SZ) is a severe chronic mental disorder with a high heritability. Current microarray analyses typically focus on identifying differentially expressed genes or enriched pathways relevant to phenotypes. Whether there is a variability change of the genomic transcription in diseases has rarely been explored. In this study, we compared coefficient of variation (CV, the ratio of the standard deviation to the mean) of genome transcription of early-onset SZ (EOS) patients with controls in a blood mRNA microarray dataset and a blood microRNA (miRNA) microarray dataset. Furthermore, we compared CV of the expression levels of 17 genes in blood of the 30 patients before and after the 12-week treatment using real-time quantitative PCR (RT-qPCR) analysis. Our results indicated a significant increase of CV of genome transcription in patients compared with controls in both the mRNA and the miRNA datasets. The 30 after-treatment patients showed a significant decrease of CV of gene expression compared with the before-treatment patients. Our study may implicate the blood gene expression variability in SZ, providing further evidence supporting the abnormality of peripheral blood transcriptome in SZ. Given that peripheral blood can be easily collected from patients and followed longitudinally, our results may indicate a new way to facilitate the identification of the signatures of clinical subtypes, their prognosis and treatment response.
authors.affiliation: Wuxi Mental Health Center, Nanjing Medical University, Wuxi, Jiangsu Province, China.
authors.forename: Fuquan
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Unit on Statistical Genomics, Division of Intramural Research Program, National Institute of Mental Health, National Institutes of Health, Bethesda, Maryland, United States of America.
authors.forename: Yin
authors.initials: Y
authors.lastname: Yao Shugart
authors.affiliation: Institute of Mental Health, Sixth Hospital, Peking University; Beijing 100191, China.|Key Laboratory of Mental Health, Ministry of Health &National Clinical Research Center for Mental Disorders (Peking University), Beijing, 100191, China.
authors.forename: Weihua
authors.initials: W
authors.lastname: Yue
authors.affiliation: Wuxi Mental Health Center, Nanjing Medical University, Wuxi, Jiangsu Province, China.
authors.forename: Zaohuo
authors.initials: Z
authors.lastname: Cheng
authors.affiliation: Wuxi Mental Health Center, Nanjing Medical University, Wuxi, Jiangsu Province, China.
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Wang
authors.affiliation: Wuxi Mental Health Center, Nanjing Medical University, Wuxi, Jiangsu Province, China.
authors.forename: Zhenhe
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: Wuxi Mental Health Center, Nanjing Medical University, Wuxi, Jiangsu Province, China.
authors.forename: Chunhui
authors.initials: C
authors.lastname: Jin
authors.affiliation: Wuxi Mental Health Center, Nanjing Medical University, Wuxi, Jiangsu Province, China.
authors.forename: Jianmin
authors.initials: J
authors.lastname: Yuan
authors.affiliation: Department of Psychiatry, First Hospital /First Clinical Medical College of Shanxi Medical University, Taiyuan, China.
authors.forename: Sha
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Psychiatry, First Hospital /First Clinical Medical College of Shanxi Medical University, Taiyuan, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Xu
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects; D014644:Genetic Variation; D023281:Genomics / Q000379:methods; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics; D008875:Middle Aged; D012333:RNA, Messenger / Q000235:genetics; D012559:Schizophrenia / Q000188:drug therapy / Q000235:genetics*; D014158:Transcription, Genetic; D059467:Transcriptome; D055815:Young Adult
chemical_list: D035683:MicroRNAs; D012333:RNA, Messenger