title: Hydrophilic Interaction Liquid Chromatography/Mass Spectrometry: An Attractive and Prospective Method for the Quantitative Bioanalysis in Drug Metabolism.
abstract: During the development, dosage optimization and safety evaluation of a drug, rapid and precise monitoring of administered drug and/or its metabolites in biological samples including blood, plasma, serum, tissues and saliva are vital. As drug biotransformation produces more hydrophilic metabolites for the enhancement of drug elimination, which is often a challenge for traditional reversed-phase liquid chromatography (RPLC) separation. Because hydrophilic interaction liquid chromatography (HILIC) is capable of retaining polar compounds and readily compatible with mass spectrometry (MS), HILIC has been used as a complementary separation technique to RPLC for analysis of polar metabolites, especially polar drugs and their metabolites. This review covers core aspects of HILIC-MS/MS method and overall profile of its application in analysis of drug and/or its metabolites. The emphasis of this paper has been placed on the applications of HILIC-MS/MS method in quantitative bioanalysis of drugs alone or along with their metabolites in drug metabolism studies in recent years. As a fundamental and critical step of bioanalytical method, conventional sample preparation techniques of biological matrices for the HILIC-MS/MS analysis of drugs and/or their metabolites are also briefly featured.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Li
authors.forename: Jie
authors.initials: J
authors.lastname: Han
authors.forename: Shi-an
authors.initials: SA
authors.lastname: Sun
authors.forename: Kai
authors.initials: K
authors.lastname: Chen
authors.affiliation: Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical College, Xuzhou 221004, China. tdq993@hotmail.com.
authors.forename: Dao-quan
authors.initials: DQ
authors.lastname: Tang
mesh_terms: D000700:Analgesics / Q000032:analysis / Q000378:metabolism; D053000:Analytic Sample Preparation Methods; D000900:Anti-Bacterial Agents / Q000032:analysis / Q000378:metabolism; D016588:Anticarcinogenic Agents / Q000032:analysis / Q000378:metabolism; D000998:Antiviral Agents / Q000032:analysis / Q000378:metabolism; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008658:Inactivation, Metabolic; D004364:Pharmaceutical Preparations / Q000032:analysis* / Q000378:metabolism*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000700:Analgesics; D000900:Anti-Bacterial Agents; D016588:Anticarcinogenic Agents; D000998:Antiviral Agents; D004364:Pharmaceutical Preparations