title: Community Structure of Arbuscular Mycorrhizal Fungi in Rhizospheric Soil of a Transgenic High-Methionine Soybean and a Near Isogenic Variety.
abstract: The use of transgenic plants in agriculture provides many economic benefits, but it also raises concerns over the potential impact of transgenic plants on the environment. We here examined the impact of transgenic high-methionine soybean ZD91 on the arbuscular mycorrhizal (AM) fungal community structure in rhizosphere soil. Our investigations based on clone libraries were conducted in field trials at four growth stages of the crops each year from 2012 to 2013. A total of 155 operational taxonomic units (OTUs) of AM fungi were identified based on the sequences of small subunit ribosomal RNA (SSU rRNA) genes. There were no significant differences found in AM fungal diversity in rhizosphere soil during the same growth stage between transgenic soybean ZD91 and its non-transgenic parental soybean ZD. In addition, plant growth stage and year had the strongest effect on the AM fungal community structure while the genetically modified (GM) trait studied was the least explanatory factor. In conclusion, we found no indication that transgenic soybean ZD91 cultivation poses a risk for AM fungal communities in agricultural soils.
authors.affiliation: Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, and Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing, China.|Development Center of Science and Technology, Ministry of Agriculture, Beijing, China.
authors.forename: Jingang
authors.initials: J
authors.lastname: Liang
authors.affiliation: Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, and Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Meng
authors.affiliation: The National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI), MOA Key Laboratory of Soybean Biology (Beijing), Institute of Crop Science, The Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Shi
authors.initials: S
authors.lastname: Sun
authors.affiliation: The National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI), MOA Key Laboratory of Soybean Biology (Beijing), Institute of Crop Science, The Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Cunxiang
authors.initials: C
authors.lastname: Wu
authors.affiliation: Nanchong Academy of Agricultural Science, Nanchong, China.
authors.forename: Haiying
authors.initials: H
authors.lastname: Wu
authors.affiliation: Nanchong Academy of Agricultural Science, Nanchong, China.
authors.forename: Mingrong
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, and Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing, China.
authors.forename: Haifeng
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, and Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing, China.
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Zheng
authors.affiliation: Agro-biotechnology Research Institute, Jilin Academy of Agriculture Sciences, Changchun, China.
authors.forename: Xinyuan
authors.initials: X
authors.lastname: Song
authors.affiliation: Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, and Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing, China.
authors.forename: Zhengguang
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D004271:DNA, Fungal / Q000302:isolation & purification / Q000378:metabolism; D008715:Methionine / Q000378:metabolism*; D038821:Mycorrhizae / Q000145:classification / Q000235:genetics / Q000254:growth & development*; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology; D030821:Plants, Genetically Modified / Q000378:metabolism* / Q000382:microbiology; D016133:Polymerase Chain Reaction; D025341:Principal Component Analysis; D058441:Rhizosphere; D012988:Soil Microbiology; D013025:Glycine max / Q000235:genetics / Q000382:microbiology*
chemical_list: D004271:DNA, Fungal; D008715:Methionine