title: RNA-sequencing Reveals Global Transcriptomic Changes in Nicotiana tabacum Responding to Topping and Treatment of Axillary-shoot Control Chemicals.
abstract: Removal of terminal buds (topping) and control of the formation of axillary shoots (suckers) are common agronomic practices that significantly impact the yield and quality of various crop plants. Application of chemicals (suckercides) to plants following topping is an effective method for sucker control. However, our current knowledge of the influence of topping, and subsequent suckercide applications, to gene expression is limited. We analyzed the differential gene expression using RNA-sequencing in tobacco (Nicotiana tabacum) that are topped, or treated after topping by two different suckercides, the contact-localized-systemic, Flupro(®) (FP), and contact, Off-Shoot-T(®). Among the differentially expressed genes (DEGs), 179 were identified as common to all three conditions. DEGs, largely related to wounding, phytohormone metabolism and secondary metabolite biosynthesis, exhibited significant upregulation following topping, and downregulation after suckercide treatments. DEGs related to photosynthetic processes were repressed following topping and suckercide treatments. Moreover, topping and FP-treatment affect the expression of auxin and cytokinin signaling pathway genes that are possibly involved in axillary shoot formation. Our results provide insights into the global change of plant gene expression in response to topping and suckercide treatments. The regulatory elements of topping-inducible genes are potentially useful for the development of a chemical-free sucker control system.
authors.affiliation: Kentucky Tobacco Research and Development Center , University of Kentucky, Lexington, KY 40546, U.S.A.
authors.forename: Sanjay K
authors.initials: SK
authors.lastname: Singh
authors.affiliation: Kentucky Tobacco Research and Development Center , University of Kentucky, Lexington, KY 40546, U.S.A.
authors.forename: Yongmei
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Kentucky Tobacco Research and Development Center , University of Kentucky, Lexington, KY 40546, U.S.A.
authors.forename: Jayadri S
authors.initials: JS
authors.lastname: Ghosh
authors.affiliation: Kentucky Tobacco Research and Development Center , University of Kentucky, Lexington, KY 40546, U.S.A.
authors.forename: Sitakanta
authors.initials: S
authors.lastname: Pattanaik
authors.affiliation: Department of Plant and Soil Sciences, University of Kentucky, Lexington, KY 40546, U.S.A.
authors.forename: Colin
authors.initials: C
authors.lastname: Fisher
authors.affiliation: South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: R J Reynolds, Inc. 950 Reynolds Blvd, Winston-Salem, NC 27102, U.S.A.
authors.forename: Darlene
authors.initials: D
authors.lastname: Lawson
authors.affiliation: Kentucky Tobacco Research and Development Center , University of Kentucky, Lexington, KY 40546, U.S.A.|Department of Plant and Soil Sciences, University of Kentucky, Lexington, KY 40546, U.S.A.
authors.forename: Ling
authors.initials: L
authors.lastname: Yuan
mesh_terms: D016000:Cluster Analysis; D019295:Computational Biology; D004260:DNA Repair; D004261:DNA Replication; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D059014:High-Throughput Nucleotide Sequencing; D053858:Metabolic Networks and Pathways; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D018520:Plant Shoots / Q000235:genetics* / Q000378:metabolism; D015203:Reproducibility of Results; D015398:Signal Transduction; D014026:Nicotiana / Q000235:genetics* / Q000378:metabolism; D059467:Transcriptome