title: [Identification and characterization of a hypothermia nitrite bacterium Pseudomonas tolaasii Y-11].
abstract: OBJECTIVE
  Denitrifying bacteria play an important role in the biological nitrogen removal process. However, there are few studies about hypothermia nitrite denitrifying bacteria. We isolated a hypothermia and aerobic nitrite-denitrifying bacterium (named as Y-11) from the long-term flooded paddy soil. Aims of this paper were to clarify the phylogeny and denitrifying characteristics of strain Y-11.
  
  
  METHODS
  Morphological observation, specific phospholipid fatty acid and 16S rRNA analysis were employed to identify strain Y-11. Denitrification characteristics of strain Y-11, such as temperature, shaking speed, initial pH and carbon source were investigated by using shaking culture in Erlenmeyer flask.
  
  
  RESULTS
  Strain Y-11 with high removal efficiency of nitrite and total nitrogen was identified as Pseudomonas tolaasii. The optimum conditions of Y-11 effectively removing nitrite nitrogen and total nitrogen were: initial pH7.0; 15 degrees C; shaking speed 200 r/min; inoculum size 1.5 x 10(8) CFU per 100 mL medium; sodium acetate as carbon source; and nitrite nitrogen 10 mg/L. Strain Y-11 can remove nitrite nitrogen and total nitrogen up to 100% and 61.28% within 48 h.
  
  
  CONCLUSIONS
  Strain Y-11 was identified as Pseudomonas tolaasii with its potential nitrite polluted water treatment during early winter and late spring.
authors.forename: Tengxia
authors.initials: T
authors.lastname: He
authors.forename: Yi
authors.initials: Y
authors.lastname: Xu
authors.forename: Zhenlun
authors.initials: Z
authors.lastname: Li
mesh_terms: D003080:Cold Temperature; D004269:DNA, Bacterial / Q000235:genetics; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D009573:Nitrites / Q000378:metabolism*; D009584:Nitrogen / Q000378:metabolism; D010802:Phylogeny; D011549:Pseudomonas / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D009573:Nitrites; D012336:RNA, Ribosomal, 16S; D009584:Nitrogen