title: Stool vs. Serum Hepatitis B Virus DNA in Patients with Chronic Hepatitis B.
abstract: BACKGROUND Serum hepatitis B virus (HBV) DNA and hepatitis B e antigen (HBeAg) liver function in patients with chronic hepatitis B (CHB) are significantly associated. A comparison of clinical significance of fecal HBV DNA and serum HBV DNA has not yet been reported. MATERIAL AND METHODS Stool and serum samples were collected from 66 patients with CHB. Fecal HBV DNA, serum HBV DNA, and intestinal microbiota DNA were detected by real-time quantitative fluorescence polymerase chain reaction (PCR). Liver function and HBeAg were analyzed. RESULTS The stool and serum HBV DNA were positively correlated (r=0.57, P=0.001). Fecal HBV DNA was higher in the HBeAg-positive group than in the HBeAg-negative group (P=0.02). Fecal HBV DNA was negatively correlated with alkaline phosphatase (ALP) (r=-0.41, P=0.001) and TBIL (r=-0.29, P=0.02), and was positively correlated with Enterococcus (r=0.38, P=0.002). Serum HBV DNA was negatively correlated with alanine aminotransferase (ALT) (r=-0.30,P=0.02), aminotransferase (AST) (r=-0.26, P=0.049), and Lactobacillus (r=-0.31, P=0.01). CONCLUSIONS These observations suggest that fecal HBV DNA and serum HBV DNA in patients with CHB have different effects. Fecal HBV DNA might be associated with changes in Enterococcus concentrations, but serum HBV DNA is not.
authors.affiliation: Department of Gastroenterology and Infectious Diseases, Anhui Medical University (The Third Affiliated Hospital), Hefei, Anhui, China (mainland).
authors.forename: Ji-Shun
authors.initials: JS
authors.lastname: Zheng
authors.affiliation: 0.
authors.forename: Meng-Meng
authors.initials: MM
authors.lastname: Chen
authors.affiliation: 0.
authors.forename: Hai-Fei
authors.initials: HF
authors.lastname: Yang
authors.affiliation: 0.
authors.forename: Xiang-Tian
authors.initials: XT
authors.lastname: Zhou
authors.affiliation: 0.
authors.forename: Yan-Yan
authors.initials: YY
authors.lastname: Liu
authors.affiliation: Department of Infectious Diseases, Anhui Medical University (The First Affiliated Hospital), Hefei, Anhui, China (mainland).
authors.forename: Jia-Bin
authors.initials: JB
authors.lastname: Li
mesh_terms: D000328:Adult; D004279:DNA, Viral / Q000032:analysis / Q000097:blood*; D005243:Feces / Q000821:virology*; D005260:Female; D006515:Hepatitis B virus / Q000235:genetics*; D019694:Hepatitis B, Chronic / Q000503:physiopathology / Q000821:virology*; D006801:Humans; D007422:Intestines / Q000382:microbiology; D008111:Liver Function Tests; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction
chemical_list: D004279:DNA, Viral