title: 4.1N suppresses hypoxia-induced epithelial-mesenchymal transition in epithelial ovarian cancer cells.
abstract: Protein 4.1N (4.1N) is a member of the protein 4.1 family and is essential for the regulation of cell adhesion, motility and signaling. Previous studies have suggested that 4.1N may serve a tumor suppressor role. However, the molecular mechanisms remain unclear. In the current study, the role of 4.1N in the downregulation of hypoxia‑induced factor 1α (HIF‑1α) under hypoxic conditions and therefore the suppression of hypoxia induced epithelial‑mesenchymal transition (EMT) was investigated. The data were obtained from overexpressed and knockdown 4.1N epithelial ovarian cancer (EOC) cell lines. It was identified that 4.1N was capable of regulating the sub‑cellular localization and expression levels of HIF‑1α, by which 4.1N served a dominant role in the suppression of hypoxia‑induced EMT and associated genes. Collectively, the data of the current study identified 4.1N as an inhibitor of hypoxia‑induced tumor progression in EOC cells and highlighted its potential role in EOC therapy.
authors.affiliation: Department of Pathology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, P.R. China.
authors.forename: Letian
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Pathology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, P.R. China.
authors.forename: Ajin
authors.initials: A
authors.lastname: Hu
authors.affiliation: Department of Pathology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, P.R. China.
authors.forename: Mengrui
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Histology and Embryology, Peking University Health Science Center, Beijing 100191, P.R. China.
authors.forename: Hongquan
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Histology and Embryology, Peking University Health Science Center, Beijing 100191, P.R. China.
authors.forename: Caixia
authors.initials: C
authors.lastname: Ren
authors.affiliation: College of Life Science, Zhengzhou University, Zhengzhou, Henan 450051, P.R. China.
authors.forename: Xiuli
authors.initials: X
authors.lastname: An
authors.affiliation: Department of Pathology, School of Basic Medical Sciences, Third Hospital, Peking University Health Science Center, Beijing 100191, P.R. China.
authors.forename: Congrong
authors.initials: C
authors.lastname: Liu
mesh_terms: D015820:Cadherins / Q000378:metabolism; D000077216:Carcinoma, Ovarian Epithelial; D015687:Cell Hypoxia; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000378:metabolism; D002470:Cell Survival; D003598:Cytoskeletal Proteins / Q000378:metabolism*; D058750:Epithelial-Mesenchymal Transition; D005260:Female; D055785:Gene Knockdown Techniques; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism*; D008954:Models, Biological; D009375:Neoplasms, Glandular and Epithelial / Q000378:metabolism* / Q000473:pathology*; D009479:Neuropeptides / Q000378:metabolism*; D010051:Ovarian Neoplasms / Q000378:metabolism* / Q000473:pathology*
chemical_list: D015820:Cadherins; D003598:Cytoskeletal Proteins; C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D008565:Membrane Proteins; D009479:Neuropeptides; C120557:erythrocyte membrane protein band 4.1-like 1