title: Antihypertensive and cardioprotective effects of Cerebralcare granule® on spontaneously hypertensive rats from the perspective of the gaseous triumvirate NO-CO-H2S system.
abstract: Cerebralcare granule(®) (CG) has been reported to have hypotensive effect. However, several pathways involved in the mechanism of hypotension are still unclear. This study was designed to verify the antihypertensive effect of CG and to characterize its mechanism of action, especially from the perspective of gasotrasmmiter NO/cGMP, CO/HO and H2S/CSE systems. By using the widely used in vitro model of rat isolated thoracic aortic rings, the vasorelaxant effect of CG were studied. Furthermore, we assessed the chronic hypotensive effect of CG on spontaneously hypertensive rats (SHRs) and further to explore the potential mechanisms of its antihypertensive activity. Data in the present study demonstrated that oral treatment with CG could induce a potent antihypertensive effect. CG could reduce the intima-media thickness (IMT) of thoracic aorta significantly and increase the serum NO and H2S levels. In addition, the present results indicated that CG played a critical protective role against pressure overload-induced cardiac hypertrophy. CG not only inhibited the development of cardiac hypertrophy but also improved ventricular function. In vitro, the results showed that CG induced relaxation in rat aortic rings through an endothelium-dependent pathway mediated by NO/cGMP, CO/HO and H2S/CSE systems. Taken together, the present study demonstrated that CG could induce a potent antihypertensive effect that was partly due to the improvement of endothelial function. Also CG played a critical protective role against pressure overload-induced cardiac hypertrophy. In addition, CG could induce relaxation in rat aortic rings.
authors.affiliation: School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China.
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Qu
authors.affiliation: Department of Pharmacy, Logistics College of Chinese People's Armed Police Forces, Tianjin 300162, China.
authors.forename: Jingze
authors.initials: J
authors.lastname: Zhang
authors.affiliation: School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China. Electronic address: biochemgao@hotmail.com.
authors.forename: Wenyuan
authors.initials: W
authors.lastname: Gao
authors.affiliation: Department of Pharmacy, Logistics College of Chinese People's Armed Police Forces, Tianjin 300162, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
authors.affiliation: School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China.
authors.forename: Hanhan
authors.initials: H
authors.lastname: Huang
authors.affiliation: School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China.
authors.forename: Liqin
authors.initials: L
authors.lastname: Huo
authors.affiliation: School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China.
authors.forename: Hongfa
authors.initials: H
authors.lastname: Li
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000959:Antihypertensive Agents / Q000008:administration & dosage* / Q000494:pharmacology; D001013:Aorta, Thoracic / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage* / Q000494:pharmacology; D006334:Heart Function Tests / Q000187:drug effects*; D006862:Hydrogen Sulfide / Q000097:blood; D006973:Hypertension / Q000097:blood / Q000188:drug therapy* / Q000503:physiopathology; D008297:Male; D009569:Nitric Oxide / Q000097:blood; D051381:Rats; D011918:Rats, Inbred SHR; D017539:Tunica Intima / Q000187:drug effects; D014665:Vasodilator Agents / Q000008:administration & dosage* / Q000494:pharmacology
chemical_list: D000959:Antihypertensive Agents; D004365:Drugs, Chinese Herbal; D014665:Vasodilator Agents; C553222:cerebralcare preparation; D009569:Nitric Oxide; D006862:Hydrogen Sulfide
keywords: Cardioprotection; Cerebralcare granule(®); Hypertension