title: [Establishment of a mouse model of acute graft-versus-host disease by busulfan combined with cyclophosphamide].
abstract: OBJECTIVE
  To develop a stable mouse model of acute graft-versus-host disease (aGVHD) by preconditioning with busulfan (BS)-cyclophospha mide (CP).
  
  
  METHODS
  Male BALB/c (H-2kd) as recipients were conditioned with 100 mg/kg BS and 200 mg/kg CP followed by being transplanted with bone marrow cells (2×10(7)) or bone marrow cells (2×10(7))-spleen cells (2×10(7)) from female C57BL/6(H-2Kb) as donors. The general data of the transplanted mice were recorded and the manifestations of aGVHD were evaluated. Histopathological changes were observed using HE staining and chimerism in spleen and bone marrow cells was studied using flow cytometry.
  
  
  RESULTS
  Mice transplanted with bone marrow cells-spleen cells developed classic aGVHD manifestations starting from the 7th day after transplantation with full donor chimerism. GVHD histopathological changes were seen in liver, spleen, intestine, skin and lung of these mice. The median survival time of these aGVHD mice was 10 days, and they all died within 30 days. Mice transplanted with only bone marrow cells survived over 45 days without any aGVHD signs.
  
  
  CONCLUSIONS
  We developed a stable and reliable aGVHD murine model successfully, which can be used to study the pathogenesis, prevention and therapeutic effect of aGVHD.
authors.affiliation: Department of Hematology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
authors.forename: Xin
authors.initials: X
authors.lastname: He
authors.affiliation: Department of Hematology, Zhongshan Hospital, Sun Yat-sen University, Zhongshan 528403, China. *Corresponding authors, E-mail: wodeyoujian@foxmail.com.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Xu
authors.affiliation: Department of Hematology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Hematology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
authors.forename: Wenfang
authors.initials: W
authors.lastname: Yi
authors.affiliation: Department of Hematology, Zhongshan Hospital, Sun Yat-sen University, Zhongshan 528403, China.
authors.forename: Yongbin
authors.initials: Y
authors.lastname: Ye
authors.affiliation: Department of Hematology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
authors.forename: Yuxian
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Department of Hematology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China. *Corresponding authors, E-mail: doctorxu@163.com.
authors.forename: Kunyuan
authors.initials: K
authors.lastname: Guo
mesh_terms: D000818:Animals; D016026:Bone Marrow Transplantation; D002066:Busulfan / Q000008:administration & dosage / Q000009:adverse effects*; D003520:Cyclophosphamide / Q000008:administration & dosage / Q000009:adverse effects*; D004195:Disease Models, Animal; D005260:Female; D006086:Graft vs Host Disease / Q000139:chemically induced / Q000401:mortality / Q000473:pathology*; D006801:Humans; D008099:Liver / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D013154:Spleen / Q000473:pathology; D014184:Transplantation, Homologous
chemical_list: D003520:Cyclophosphamide; D002066:Busulfan