title: Diagnosis and management of aspergillosis in the Netherlands: a national survey.
abstract: A survey of diagnosis and treatment of invasive aspergillosis was conducted in eight University Medical Centers (UMCs) and eight non-academic teaching hospitals in the Netherlands. Against a background of emerging azole resistance in Aspergillus fumigatus routine resistance screening of clinical isolates was performed primarily in the UMCs. Azole resistance rates at the hospital level varied between 5% and 10%, although rates up to 30% were reported in high-risk wards. Voriconazole remained first choice for invasive aspergillosis in 13 out of 16 hospitals. In documented azole resistance 14 out of 16 centres treated patients with liposomal amphotericin B.
authors.affiliation: Department of Medical Microbiology, Radboud University Medical Center, Nijmegen, The Netherlands.|Department of Medical Microbiology and Infectious Diseases, Canisius Wilhelmina Hospital, Nijmegen, The Netherlands.|National Mycology Reference Laboratory, Nijmegen, The Netherlands.
authors.forename: Pieter P A
authors.initials: PP
authors.lastname: Lestrade
authors.affiliation: Department of Medical Microbiology, Radboud University Medical Center, Nijmegen, The Netherlands.|Department of Medical Microbiology and Infectious Diseases, Canisius Wilhelmina Hospital, Nijmegen, The Netherlands.|National Mycology Reference Laboratory, Nijmegen, The Netherlands.
authors.forename: Jacques F
authors.identifier: http://orcid.org/0000-0003-3253-6080
authors.initials: JF
authors.lastname: Meis
authors.affiliation: Department of Medical Microbiology, University Medical Centre Groningen, Groningen, The Netherlands.
authors.forename: Jan P
authors.initials: JP
authors.lastname: Arends
authors.affiliation: Department of Medical Microbiology, Leiden University Medical Centre, Leiden, The Netherlands.
authors.forename: Martha T
authors.initials: MT
authors.lastname: van der Beek
authors.affiliation: Department of Medical Microbiology, Atrium-Orbis Medical Centre, Heerlen, The Netherlands.
authors.forename: Els
authors.initials: E
authors.lastname: de Brauwer
authors.affiliation: Department of Medical Microbiology and Infection Control, VU University Medical Centre, Amsterdam, The Netherlands.
authors.forename: Karin
authors.initials: K
authors.lastname: van Dijk
authors.affiliation: Centre for Infectious Disease Control, National Institute for Public Health and the Environment, Bilthoven, The Netherlands.
authors.forename: Sabine C
authors.initials: SC
authors.lastname: de Greeff
authors.affiliation: Department of Medical Microbiology, University Medical Centre Utrecht, Utrecht, The Netherlands.
authors.forename: Pieter-Jan
authors.initials: PJ
authors.lastname: Haas
authors.affiliation: Department of Medical Microbiology, Academic Medical Centre, Amsterdam, The Netherlands.
authors.forename: Caspar J
authors.initials: CJ
authors.lastname: Hodiamont
authors.affiliation: Department of Medical Microbiology, Leiden University Medical Centre, Leiden, The Netherlands.
authors.forename: Ed J
authors.initials: EJ
authors.lastname: Kuijper
authors.affiliation: Centre for Infectious Disease Control, National Institute for Public Health and the Environment, Bilthoven, The Netherlands.
authors.forename: Tjalling
authors.initials: T
authors.lastname: Leenstra
authors.affiliation: Department of Medical Microbiology, Medical Centre Haaglanden, The Hague, The Netherlands.
authors.forename: Anouk E
authors.initials: AE
authors.lastname: Muller
authors.affiliation: Department of Medical Microbiology, Maastricht University Medical Centre, Maastricht, The Netherlands.
authors.forename: Astrid M L
authors.initials: AM
authors.lastname: Oude Lashof
authors.affiliation: Department of Medical Microbiology and Infectious Diseases, Erasmus Medical Centre, Rotterdam, The Netherlands.
authors.forename: Bart J
authors.initials: BJ
authors.lastname: Rijnders
authors.affiliation: Laboratory for Infectious Diseases, Groningen, The Netherlands.
authors.forename: Eveline
authors.initials: E
authors.lastname: Roelofsen
authors.affiliation: Department of Medical Microbiology, Medisch Centrum Alkmaar, Alkmaar, The Netherlands.
authors.forename: Wouter
authors.initials: W
authors.lastname: Rozemeijer
authors.affiliation: Department of Medical Microbiology, Sint Antonius Hospital, Nieuwegein, The Netherlands.
authors.forename: Mathijs
authors.initials: M
authors.lastname: Tersmette
authors.affiliation: Department of Medical Microbiology, Leiden University Medical Centre, Leiden, The Netherlands.
authors.forename: Elizabeth M
authors.initials: EM
authors.lastname: Terveer
authors.affiliation: Department of Medical Microbiology, Amphia Hospital, Breda, The Netherlands.
authors.forename: Cees M
authors.initials: CM
authors.lastname: Verduin
authors.affiliation: Department of Medical Microbiology, Isala Hospital, Zwolle, The Netherlands.
authors.forename: Maurice J H M
authors.initials: MJ
authors.lastname: Wolfhagen
authors.affiliation: Department of Medical Microbiology, Radboud University Medical Center, Nijmegen, The Netherlands.
authors.forename: Willem J G
authors.initials: WJ
authors.lastname: Melchers
authors.affiliation: Department of Medical Microbiology, Radboud University Medical Center, Nijmegen, The Netherlands.|National Mycology Reference Laboratory, Nijmegen, The Netherlands.
authors.forename: Paul E
authors.initials: PE
authors.lastname: Verweij
mesh_terms: D000666:Amphotericin B / Q000627:therapeutic use*; D000935:Antifungal Agents / Q000627:therapeutic use; D001228:Aspergillosis / Q000175:diagnosis* / Q000188:drug therapy* / Q000453:epidemiology; D001232:Aspergillus fumigatus / Q000187:drug effects* / Q000302:isolation & purification; D025141:Drug Resistance, Fungal; D006801:Humans; D009426:Netherlands / Q000453:epidemiology; D011795:Surveys and Questionnaires; D065819:Voriconazole / Q000494:pharmacology / Q000627:therapeutic use*
chemical_list: D000935:Antifungal Agents; C068538:liposomal amphotericin B; D000666:Amphotericin B; D065819:Voriconazole
keywords: Aspergillosis; azole resistance; treatment