title: Quadruplex DNA-Stabilising Dinuclear Platinum(II) Terpyridine Complexes with Flexible Linkers.
abstract: Four dinuclear terpyridineplatinum(II) (Pt-terpy) complexes were investigated for interactions with G-quadruplex DNA (QDNA) and duplex DNA (dsDNA) by synchrotron radiation circular dichroism (SRCD), fluorescent intercalator displacement (FID) assays and fluorescence resonance energy transfer (FRET) melting studies. Additionally, computational docking studies were undertaken to provide insight into potential binding modes for these complexes. The complexes demonstrated the ability to increase the melting temperature of various QDNA motifs by up to 17 °C and maintain this in up to a 600-fold excess of dsDNA. This study demonstrates that dinuclear Pt-terpy complexes stabilise QDNA and have a high degree of selectivity for QDNA over dsDNA.
authors.affiliation: Nanoscale Organisation and Dynamics Group, School of Science and Health, Western Sydney University, Penrith, NSW 2751, Australia.
authors.forename: Dale L
authors.initials: DL
authors.lastname: Ang
authors.affiliation: Nanoscale Organisation and Dynamics Group, School of Science and Health, Western Sydney University, Penrith, NSW 2751, Australia.
authors.forename: Benjamin W J
authors.initials: BW
authors.lastname: Harper
authors.affiliation: Department of Chemistry, Imperial College London, London, SW7 2AZ, UK.
authors.forename: Leticia
authors.initials: L
authors.lastname: Cubo
authors.affiliation: Department of Chemistry, Imperial College London, London, SW7 2AZ, UK.
authors.forename: Oscar
authors.initials: O
authors.lastname: Mendoza
authors.affiliation: Department of Chemistry, Imperial College London, London, SW7 2AZ, UK.|Institute of Chemical Biology, Imperial College London, London, SW7 2AZ, UK.
authors.forename: Ramon
authors.identifier: http://orcid.org/0000-0003-2992-199X
authors.initials: R
authors.lastname: Vilar
authors.affiliation: Nanoscale Organisation and Dynamics Group, School of Science and Health, Western Sydney University, Penrith, NSW 2751, Australia. J.Aldrich-Wright@uws.edu.au.
authors.forename: Janice
authors.identifier: http://orcid.org/0000-0002-6943-6908
authors.initials: J
authors.lastname: Aldrich-Wright
mesh_terms: D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer; D054856:G-Quadruplexes; D009944:Organoplatinum Compounds / Q000737:chemistry*
chemical_list: D009944:Organoplatinum Compounds; D004247:DNA
keywords: DNA; FRET; G-quadruplexes; circular dichroism; platinum