title: [Enhancing erythromycin precursor 6-dEB production by using synthetic small regulatory RNAs in Escherichia coli].
abstract: Although heterologous biosynthesis of polyketide erythromycin has been successfully achieved in Escherichia coli, the titer remains at a very low level (-10 mg/L). In this study, based on genome-scale metabolic model of E. coli, in silico method flux distribution comparison analysis was used to discover novel potential targets for heterologous 6-dEB biosynthesis. Synthetic small regulatory RNAs (sRNAs) was used to experimentally test 12 down-regulated targets. The results showed that repression of each of these target genes e.g. lsrC and ackA led to significantly improve heterologous 6-dEB biosynthesis. Using co-repression of lsrC and ackA, 6-dEB titer was improved by 59.9% in shake-flask with a maximum yield of 22.8 mg/L. This study indicates that combined flux distribution comparison analysis and synthetic small regulatory RNAs is an effective strategy to improve 6-dEB production in E. coli.
authors.forename: Shujie
authors.initials: S
authors.lastname: Song
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Xiong
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
mesh_terms: D015536:Down-Regulation; D004917:Erythromycin / Q000096:biosynthesis*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D007218:Industrial Microbiology / Q000379:methods*; D012313:RNA
chemical_list: D012313:RNA; D004917:Erythromycin