title: Visualizing Human Hematopoietic Stem Cell Trafficking In Vivo Using a Zebrafish Xenograft Model.
abstract: Zebrafish is gaining increased popularity as a model organism to study stem cell biology. It also is widely used as model system to visualize human leukemic stem cells. However, xenotransplantation of primary human stem/progenitor cells has not been described. Here, we use casper pigmentation mutant fish that are transparent crossed to fli-GFP transgenic fish as recipients of red labeled human CD34(+) cells. We have investigated various conditions and protocols with the aim to monitor and visualize the fate of transplanted human CD34(+) cells. We here report successful use of casper mutant zebrafish embryos for the direct monitoring of human hematopoietic stem cell transplantation, differentiation, and trafficking in vivo.
authors.affiliation: 1 Department of Immunohematology and Blood Transfusion, Leiden University Medical Center , Leiden, the Netherlands .
authors.forename: Frank J T
authors.initials: FJ
authors.lastname: Staal
authors.affiliation: 2 Department of Molecular Cell Biology, Institute of Biology Leiden, Leiden University , Leiden, the Netherlands .
authors.forename: Herman P
authors.initials: HP
authors.lastname: Spaink
authors.affiliation: 1 Department of Immunohematology and Blood Transfusion, Leiden University Medical Center , Leiden, the Netherlands .
authors.forename: Willem E
authors.initials: WE
authors.lastname: Fibbe
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D019070:Cell Lineage; D002465:Cell Movement; D058948:Cell Tracking / Q000379:methods*; D004625:Embryo, Nonmammalian / Q000166:cytology; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D064593:Heterografts / Q000166:cytology*; D006801:Humans; D008954:Models, Biological; D017154:Stromal Cells / Q000378:metabolism; D015027:Zebrafish / Q000378:metabolism*