title: Rapid Electroanalytical Method for Determination of Nebivolol at a Boron-Doped Diamond Electrode.
abstract: A boron-doped diamond electrode provided a sensitive and cost-effective sensing platform for detection and quantitative determination of novel beta(1)-adrenergic receptor antagonist nebivolol. The net square-wave voltammetric response at 1.31 V related to the oxidation of nebivolol was obtained in Britton-Robinson buffer solution at pH 8. It increased linearly with the drug concentration in the range of 2.5×10(-7) to 1.5×10(-5) M. The LOD attained was 3.2×10(-8) M. The practical analytical approach was illustrated by high speed quantification of nebivolol in a commercial pharmaceutical formulation. The RP-HPLC was selected as a comparative method for evaluating the proposed electroanalytical method. The newly developed method at the unmodified electrode surface was faster and simpler in comparison with HPLC (the retention time was 17.6 min), and only 6 s was necessary for direct voltammetric measurement in the potential range from 0.5 to 1.7 V with a 2 mV potential step and pulse frequency of 100 Hz.
authors.affiliation: University of Zagreb, Faculty of Pharmacy and Biochemistry, A. Kovacica 1, 10000 Zagreb, Croatia.
authors.forename: Biljana
authors.initials: B
authors.lastname: Nigović
authors.forename: Ana
authors.initials: A
authors.lastname: Mornar
authors.forename: Mario
authors.initials: M
authors.lastname: Završki
mesh_terms: D001895:Boron / Q000737:chemistry*; D018130:Diamond / Q000737:chemistry*; D055664:Electrochemical Techniques; D004566:Electrodes; D006863:Hydrogen-Ion Concentration; D000068577:Nebivolol / Q000032:analysis*
chemical_list: D000068577:Nebivolol; D018130:Diamond; D001895:Boron