title: Development of SCAR Markers Based on Improved RAPD Amplification Fragments and Molecular Cloning for Authentication of Herbal Medicines Angelica sinensis, Angelica acutiloba and Levisticum officinale.
abstract: Molecular cloning from DNA fragments of improved RAPD amplification of Angelica sinensis, Angelica acutiloba and Levisticum officinale, provided novel sequence-characterized amplified region (SCAR) markers A13, A23, A1-34 and A1-0 whose sequences were deposited in the GenBank database with the accession numbers KP641315, KP641316, KP641317 and KP641318, respectively. By optional PCR amplification, the SCAR markers A13 and A23 are Levisticum officinale-specific, whereas the SCAR marker A1-34 is Angelica acutiloba-specific, and the SCAR marker A1-0 is Angelica sinensis-specific. These diagnostic SCAR markers may be useful for genetic authentications, for ecological conservation of all three medicinal plants and as a helpful tool for the genetic authentication of adulterant samples.
authors.forename: Chun
authors.initials: C
authors.lastname: Zhang
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Mei
authors.forename: Jingliang
authors.initials: J
authors.lastname: Cheng
authors.forename: Yin
authors.initials: Y
authors.lastname: He
authors.forename: Md Asaduzzaman
authors.initials: MA
authors.lastname: Khan
authors.forename: Peiyi
authors.initials: P
authors.lastname: Luo
authors.forename: Saber
authors.initials: S
authors.lastname: Imani
authors.forename: Junjiang
authors.initials: J
authors.lastname: Fu
mesh_terms: D029969:Angelica / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D002681:China; D003001:Cloning, Molecular; D018744:DNA, Plant / Q000235:genetics; D003710:Demography; D005819:Genetic Markers; D030003:Levisticum / Q000737:chemistry / Q000235:genetics*; D028321:Plant Preparations / Q000737:chemistry*; D019105:Random Amplified Polymorphic DNA Technique
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers; D028321:Plant Preparations