title: Size-based characterization of nanoparticle mixtures by the inline coupling of capillary electrophoresis to Taylor dispersion analysis.
abstract: Separation of closely related nanoparticles is still a challenging issue for the characterization of complex mixtures for industrial/research applications or regulatory purposes. In this work, the remarkable separating performances of CE were complemented with the absolute size-based determination provided by Taylor dispersion analysis (TDA) for the characterization of nanoparticle mixtures. The inline hyphenation of CE to TDA was successfully implemented for the baseline separation followed by a size-based characterization of a bimodal mixture containing two closely size-related nanolatexes (70nm and 56nm radii). A pixel sensor UV area imager providing three detection points along the capillary was used for a differential measurement of the peak broadening during the Taylor dispersion step. Comparison of this new technique with dynamic light scattering and hydrodynamic chromatography is also discussed.
authors.affiliation: Université Grenoble Alpes, DPM UMR 5063, CNRS, F-38041 Grenoble, France.
authors.forename: Farid
authors.initials: F
authors.lastname: Oukacine
authors.affiliation: BASF SE, GKD/P-B001, 67056 Ludwigshafen, Germany.
authors.forename: Aurélie
authors.initials: A
authors.lastname: Morel
authors.affiliation: Institut des Biomolécules Max Mousseron (IBMM, UMR 5247 CNRS, Université de Montpellier, Ecole Nationale Supérieure de Chimie de Montpellier), Place Eugène Bataillon, CC 1706, 34095 Montpellier Cedex 5, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Desvignes
authors.affiliation: Institut des Biomolécules Max Mousseron (IBMM, UMR 5247 CNRS, Université de Montpellier, Ecole Nationale Supérieure de Chimie de Montpellier), Place Eugène Bataillon, CC 1706, 34095 Montpellier Cedex 5, France. Electronic address: herve.cottet@umontpellier.fr.
authors.forename: Hervé
authors.initials: H
authors.lastname: Cottet
mesh_terms: D002845:Chromatography; D004058:Diffusion; D019075:Electrophoresis, Capillary / Q000379:methods*; D057446:Hydrodynamics; D053758:Nanoparticles / Q000032:analysis*; D010316:Particle Size
keywords: Capillary electrophoresis; Diffusion coefficient; Hydrodynamic radius; Multiple UV detection points; Nanoparticles; Taylor dispersion analysis