title: Classification of Pelteobagrus fish in Poyang Lake based on mitochondrial COI gene sequence.
abstract: We use DNA molecular marker technology to correct the deficiency of traditional morphological taxonomy. Totality 770 Pelteobagrus fish from Poyang Lake were collected. After preliminary morphological classification, random selected eight samples in each species for DNA extraction. Mitochondrial COI gene sequence was cloned with universal primers and sequenced. The results showed that there are four species of Pelteobagrus living in Poyang Lake. The average of intraspecific genetic distance value was 0.003, while the average interspecific genetic distance was 0.128. The interspecific genetic distance is far more than intraspecific genetic distance. Besides, phylogenetic tree analysis revealed that molecular systematics was in accord with morphological classification. It indicated that COI gene is an effective DNA molecular marker in Pelteobagrus classification. Surprisingly, the intraspecific difference of some individuals (P. e6, P. n6, P. e5, and P. v4) from their original named exceeded species threshold (2%), which should be renewedly classified into Pelteobagrus fulvidraco. However, another individual P. v3 was very different, because its genetic distance was over 8.4% difference from original named Pelteobagrus vachelli. Its taxonomic status remained to be further studied.
authors.affiliation: a Department of Bioscience , College of Life Science, Nanchang University , Nanchang , China and.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhong
authors.affiliation: a Department of Bioscience , College of Life Science, Nanchang University , Nanchang , China and.
authors.forename: Ting-Ting
authors.initials: TT
authors.lastname: Chen
authors.affiliation: a Department of Bioscience , College of Life Science, Nanchang University , Nanchang , China and.
authors.forename: Rui-Yue
authors.initials: RY
authors.lastname: Gong
authors.affiliation: a Department of Bioscience , College of Life Science, Nanchang University , Nanchang , China and.
authors.forename: Zhe-Xia
authors.initials: ZX
authors.lastname: Zhao
authors.affiliation: a Department of Bioscience , College of Life Science, Nanchang University , Nanchang , China and.
authors.forename: Binhua
authors.initials: B
authors.lastname: Wang
authors.affiliation: b Institute of Fisheries Science of Jiangxi Province , Nanchang , China.
authors.forename: Chunlin
authors.initials: C
authors.lastname: Fang
authors.affiliation: a Department of Bioscience , College of Life Science, Nanchang University , Nanchang , China and.
authors.forename: Hui-Ling
authors.initials: HL
authors.lastname: Mao
mesh_terms: D000818:Animals; D002397:Catfishes / Q000145:classification / Q000235:genetics*; D016000:Cluster Analysis; D004272:DNA, Mitochondrial / Q000302:isolation & purification / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D050259:Genes, Mitochondrial; D060106:Lakes; D010802:Phylogeny
chemical_list: D004272:DNA, Mitochondrial; D003576:Electron Transport Complex IV
keywords: Barcoding; Pelteobagrus fish; Poyang Lake; classification; mitochondrial COI