title: Protection mechanism of early hyperbaric oxygen therapy in rats with permanent cerebral ischemia.
abstract: [Purpose] The purpose of this study was to investigate whether early hyperbaric oxygen is useful in rats with permanent cerebral ischemia, and whether its mechanism relates to the inhibition of the tumor necrosis factor-alpha-protein kinase C-alpha pathway. [Subjects] Healthy, male Sprague-Dawley rats (N = 108) were the subjects. [Methods] After middle cerebral artery occlusion models were successfully made, rats were randomly divided into sham-operated, cerebral ischemia, and hyperbaric oxygen groups. At 4 and 12 hours after modeling, the volume of cerebral infarction was determined by triphenyltetrazolium chloride staining, and brain water content was measured using the dry and wet method. The expression of tumor necrosis factor-alpha and protein kinase C-alpha in the ischemic penumbra tissue was measured using Western blot analysis. [Results] The data showed that at 4 and 12 hours after modeling, cerebral infarct volume and brain water content decreased in the hyperbaric oxygen group, and expression of tumor necrosis factor-alpha and phospho-protein kinase C-alpha in the ischemic penumbra tissue also decreased. [Conclusion] Our study demonstrates that early hyperbaric oxygen therapy has protective effects on brain tissue after cerebral ischemia, possibly via inhibition of tumor necrosis factor-alpha and phospho-protein kinase C-alpha.
authors.affiliation: Department of Rehabilitation, Shengjing Hospital of China Medical University, China.
authors.forename: Min
authors.initials: M
authors.lastname: Yu
authors.affiliation: Department of Neurobiology, College of Basic Medical Sciences, China Medical University, China.
authors.forename: Yixue
authors.initials: Y
authors.lastname: Xue
authors.affiliation: Department of Rehabilitation, Shengjing Hospital of China Medical University, China.
authors.forename: Weidi
authors.initials: W
authors.lastname: Liang
authors.affiliation: Department of Rehabilitation, Shengjing Hospital of China Medical University, China.
authors.forename: Yupeng
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Rehabilitation, Shengjing Hospital of China Medical University, China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Zhang
keywords: Cerebral ischemic rats; Cerebrovascular protection; Hyperbaric oxygen therapy