title: [The expression and significance of VIP and its receptor in the cochlea of different degrees of chronic alcoholism rats].
abstract: OBJECTIVE
  To determine whether chronic alcoholism alters the expression levels of Vasoactive intestinal polypeptide (VIP) and its receptor (VIPR1) in the cochlea of chronic alcoholism rats.
  
  
  METHODS
  We measured their expression levels in 30 SD rats, in which we created models of different degrees of chronic alcoholism. We investigated the presence of the mRNA of VIP in the cochlea of chronic alcoholism rats and controls by reverse transcription-polymerase chain reaction (RT-PCR) method. We investigated the presence of proteins of VIPR1 in poisoned rats and controls by western blot. We also evaluated the local distribution of VIP cells by immunohistochemistry.
  
  
  RESULTS
  We found that the levels of VIP and VIPR1 were downregulated in the chronic alcoholism groups compared to the controls group. The differences in some expression levels were significant different between chronic alcoholism rats and control rats. Moreover, at different degrees of alcohol poisoning in rats, the contents of VIP and VIPR1 differed. Decreased levels of VIP and VIPR1 were detected in the deep chronic alcoholism group compared to the group with low-degree poisoning (P < 0.05). In spiral ganglion cell plasm the expression of VIP and VIPR1 had no significant difference in three groups (P > 0.05).
  
  
  CONCLUSIONS
  These results suggest that VIP and VIPR1 play an important role in the auditory function in rats with chronic alcoholism. Chronic alcoholism may cause a peptide hormone secretion imbalance in the auditory system, eventually leading to hearing loss.
authors.forename: Jing
authors.initials: J
authors.lastname: Feng
authors.forename: Haibing
authors.initials: H
authors.lastname: Liu
mesh_terms: D000437:Alcoholism / Q000378:metabolism*; D000818:Animals; D003051:Cochlea / Q000378:metabolism*; D004195:Disease Models, Animal; D015536:Down-Regulation; D012333:RNA, Messenger; D051381:Rats; D017207:Rats, Sprague-Dawley; D051238:Receptors, Vasoactive Intestinal Polypeptide, Type I / Q000378:metabolism*; D013136:Spiral Ganglion; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D051238:Receptors, Vasoactive Intestinal Polypeptide, Type I; C495508:Vipr1 protein, rat; D014660:Vasoactive Intestinal Peptide