title: Inverted light-sheet microscope for imaging mouse pre-implantation development.
abstract: Despite its importance for understanding human infertility and congenital diseases, early mammalian development has remained inaccessible to in toto imaging. We developed an inverted light-sheet microscope that enabled us to image mouse embryos from zygote to blastocyst, computationally track all cells and reconstruct a complete lineage tree of mouse pre-implantation development. We used this unique data set to show that the first cell fate specification occurs at the 16-cell stage.
authors.affiliation: Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany.
authors.forename: Petr
authors.initials: P
authors.lastname: Strnad
authors.affiliation: Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Gunther
authors.affiliation: Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany.
authors.forename: Judith
authors.initials: J
authors.lastname: Reichmann
authors.affiliation: Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany.
authors.forename: Uros
authors.initials: U
authors.lastname: Krzic
authors.affiliation: Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany.
authors.forename: Balint
authors.initials: B
authors.lastname: Balazs
authors.affiliation: Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany.
authors.forename: Gustavo
authors.initials: G
authors.lastname: de Medeiros
authors.affiliation: Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany.
authors.forename: Nils
authors.initials: N
authors.lastname: Norlin
authors.affiliation: Developmental Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany.
authors.forename: Takashi
authors.initials: T
authors.lastname: Hiiragi
authors.affiliation: Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany.
authors.forename: Lars
authors.initials: L
authors.lastname: Hufnagel
authors.affiliation: Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany.
authors.forename: Jan
authors.initials: J
authors.lastname: Ellenberg
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000166:cytology*; D021621:Imaging, Three-Dimensional / Q000295:instrumentation / Q000379:methods; D051379:Mice; D008853:Microscopy / Q000295:instrumentation* / Q000379:methods*; D059008:Time-Lapse Imaging / Q000295:instrumentation / Q000379:methods