title: [Study of screening pseudoallergenic substances of Shuanghuanglian injection].
abstract: In this study, chemistry, biology and pharmacology were combinated to screen pseudoallergenic substances of Shuang-huanglian injection (SHLI) so that to establish a scientific and systematic approach to screen pseudoallergenic substances of traditional Chinese medicine injections. The mouse pseudoallergic reaction models were used to screen the pseudoallergic reaction of SHLI's intermediate extract and the intermediate extract's component or ingredient. Among the three intermediates of Shuanghuanglian injection (extract of Scutellaria baicalensis, extract of Lonicera japonica, extract of Forsythia suspensa) , pseudoallergic action of Forsythia suspensa was the strongest, Forsythia suspesnsa's pseudoallergic reaction mainly associated with the composition with largerchemical polarity. Further it was found that forsythiaside A and arctiin which existed in the the composition with largerchemical polarity caused obvious pseudoallergic reactions. SHLI with removal forsythoside A with the technology of HPLC-MS displayed reduced pseudoallergic reaction and a significant improved safety. This study provided a scientific basis for SHLI process improvements and also offered idea and research foundation for screening pseudoallergenic substances injections in other TCM injections.
authors.forename: Yan
authors.initials: Y
authors.lastname: Yi
authors.forename: Yu-shi
authors.initials: YS
authors.lastname: Zhang
authors.forename: Chun-ying
authors.initials: CY
authors.lastname: Li
authors.forename: Hai-yu
authors.initials: HY
authors.lastname: Zhao
authors.forename: Hong-bin
authors.initials: HB
authors.lastname: Xiao
authors.forename: Gui-qin
authors.initials: GQ
authors.lastname: Li
authors.forename: Yu-ting
authors.initials: YT
authors.lastname: Lu
authors.forename: Jia-yin
authors.initials: JY
authors.lastname: Han
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhao
authors.forename: Hong-jie
authors.initials: HJ
authors.lastname: Wang
authors.forename: Nan
authors.initials: N
authors.lastname: Si
authors.forename: Ai-hua
authors.initials: AH
authors.lastname: Liang
authors.forename: Bao-lin
authors.initials: BL
authors.lastname: Bian
mesh_terms: D000818:Animals; D004342:Drug Hypersensitivity / Q000209:etiology*; D004365:Drugs, Chinese Herbal / Q000009:adverse effects* / Q000032:analysis; D005663:Furans / Q000009:adverse effects; D005960:Glucosides / Q000009:adverse effects; D006027:Glycosides / Q000009:adverse effects; D007267:Injections; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR
chemical_list: D004365:Drugs, Chinese Herbal; D005663:Furans; D005960:Glucosides; D006027:Glycosides; C516400:shuang-huang-lian; C058049:forsythiaside; C077992:arctiin