title: A microfluidic ExoSearch chip for multiplexed exosome detection towards blood-based ovarian cancer diagnosis.
abstract: Tumor-derived circulating exosomes, enriched with a group of tumor antigens, have been recognized as a promising biomarker source for cancer diagnosis via a less invasive procedure. Quantitatively pinpointing exosome tumor markers is appealing, yet challenging. In this study, we developed a simple microfluidic approach (ExoSearch) which provides enriched preparation of blood plasma exosomes for in situ, multiplexed detection using immunomagnetic beads. The ExoSearch chip offers a robust, continuous-flow design for quantitative isolation and release of blood plasma exosomes in a wide range of preparation volumes (10 μL to 10 mL). We employed the ExoSearch chip for blood-based diagnosis of ovarian cancer by multiplexed measurement of three exosomal tumor markers (CA-125, EpCAM, CD24) using a training set of ovarian cancer patient plasma, which showed significant diagnostic power (a.u.c. = 1.0, p = 0.001) and was comparable with the standard Bradford assay. This work provides an essentially needed platform for utilization of exosomes in clinical cancer diagnosis, as well as fundamental exosome research.
authors.affiliation: Department of Biological and Agricultural Engineering, Kansas State University, Manhattan, KS 66506, USA. meih@ksu.edu and Department of Chemistry, University of Kansas, Lawrence, KS 66045, USA. yongz@ku.edu.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: Department of Chemistry, University of Kansas, Lawrence, KS 66045, USA. yongz@ku.edu.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Chemistry, University of Kansas, Lawrence, KS 66045, USA. yongz@ku.edu and University of Kansas Cancer Center, Kansas City, KS 66160, USA.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zeng
authors.affiliation: Department of Biological and Agricultural Engineering, Kansas State University, Manhattan, KS 66506, USA. meih@ksu.edu and Terry C. Johnson Cancer Research Center, Kansas State University, Manhattan, KS 66506, USA.
authors.forename: Mei
authors.initials: M
authors.lastname: He
mesh_terms: D014408:Biomarkers, Tumor / Q000097:blood*; D055354:Exosomes / Q000378:metabolism* / Q000648:ultrastructure; D005260:Female; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods*; D010051:Ovarian Neoplasms / Q000097:blood*; D012680:Sensitivity and Specificity
chemical_list: D014408:Biomarkers, Tumor