title: Probing molecular choreography through single-molecule biochemistry.
abstract: Single-molecule approaches are having a dramatic impact on views of how proteins work. The ability to observe molecular properties at the single-molecule level allows characterization of subpopulations and acquisition of detailed kinetic information that would otherwise be hidden in the averaging over an ensemble of molecules. In this Perspective, we discuss how such approaches have successfully been applied to in vitro-reconstituted systems of increasing complexity.
authors.affiliation: School of Chemistry, University of Wollongong, Wollongong, New South Wales, Australia.|Centre for Medical and Molecular Bioscience, University of Wollongong, Wollongong, New South Wales, Australia.|Illawarra Health and Medical Research Institute, Wollongong, New South Wales, Australia.
authors.forename: Antoine M
authors.initials: AM
authors.lastname: van Oijen
authors.affiliation: School of Chemistry, University of Wollongong, Wollongong, New South Wales, Australia.|Centre for Medical and Molecular Bioscience, University of Wollongong, Wollongong, New South Wales, Australia.|Illawarra Health and Medical Research Institute, Wollongong, New South Wales, Australia.
authors.forename: Nicholas E
authors.identifier: http://orcid.org/0000-0002-5958-6945
authors.initials: NE
authors.lastname: Dixon
mesh_terms: D001671:Biochemistry / Q000379:methods*; D001695:Biology / Q000379:methods*; D007700:Kinetics; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D011506:Proteins