title: Prevalence and characteristics of Escherichia coli sequence type 131 and its H30 and H30Rx subclones: a multicenter study from Korea.
abstract: We investigated the prevalence and characteristics of Escherichia coli sequence type 131 (ST131) and its subclones among 268 E. coli isolates. The isolates were collected from 21 Korean hospitals without use of selection criteria and were screened for ST131 status by PCR. ST131 isolates were characterized for extended-spectrum β-lactamase variants, fluoroquinolone resistance genes, plasmid addiction systems, and replicon types. The collection's 57 identified ST131 isolates (21% of 268) were distributed disproportionately by clonal subset, as follows: 21 (37%) H30Rx, 27 (47%) H30 non-Rx, and 8 (14%) non-H30. Most (93%) ST131 isolates were ciprofloxacin resistant, and all H30 isolates had the same 5 nonsynonymous mutations in gyrA, parC, and parE. Twenty (95%) of H30Rx isolates harbored CTX-M-15, whereas only 14 (52%) of H30 non-Rx isolates harbored CTX-M-14 or CTX-M-27. Most (97%) ST131 isolates harbored IncF plasmids, but vagCD was confined exclusively to H30Rx. Our findings suggest that the distinctive characteristics of H30Rx isolates could have contributed to this subclone's recent epidemiologic success.
authors.affiliation: Department of Laboratory Medicine, College of Medicine, The Catholic University of Korea, St. Vincent's Hospital, 93 Jungbu-daero, Paldal-gu, Suwon, 442-723, Korea.
authors.forename: Soo-Young
authors.initials: SY
authors.lastname: Kim
authors.affiliation: Department of Laboratory Medicine, College of Medicine, The Catholic University of Korea, Seoul St. Mary's Hospital, 222 Banpo-daero, Seocho-gu, Seoul, 137-701, Korea. Electronic address: yjpk@catholic.ac.kr.
authors.forename: Yeon-Joon
authors.initials: YJ
authors.lastname: Park
authors.affiliation: Veterans Affairs Medical Center and University of Minnesota, Minneapolis, MN, USA.
authors.forename: James R
authors.initials: JR
authors.lastname: Johnson
authors.affiliation: Department of Laboratory Medicine, College of Medicine, The Catholic University of Korea, Seoul St. Mary's Hospital, 222 Banpo-daero, Seocho-gu, Seoul, 137-701, Korea.
authors.forename: Jin Kyung
authors.initials: JK
authors.lastname: Yu
authors.affiliation: Samkwang Medical Laboratories, 57 Baume-ro, 41-gil, Seocho-gu, Seoul, 137-887, Korea.
authors.forename: Yong-Kyun
authors.initials: YK
authors.lastname: Kim
authors.affiliation: Department of Laboratory Medicine, College of Medicine, The Catholic University of Korea, St. Vincent's Hospital, 93 Jungbu-daero, Paldal-gu, Suwon, 442-723, Korea.
authors.forename: Yeong Sic
authors.initials: YS
authors.lastname: Kim
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D024881:Drug Resistance, Bacterial; D004926:Escherichia coli / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000453:epidemiology* / Q000382:microbiology*; D024841:Fluoroquinolones / Q000494:pharmacology; D005798:Genes, Bacterial; D005838:Genotype; D006761:Hospitals; D006801:Humans; D007723:Korea / Q000453:epidemiology; D017720:Molecular Epidemiology; D058885:Multilocus Sequence Typing; D020125:Mutation, Missense; D010957:Plasmids / Q000032:analysis; D016133:Polymerase Chain Reaction; D015995:Prevalence; D001618:beta-Lactamases / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D024841:Fluoroquinolones; D001618:beta-Lactamases
keywords: CTX-M-15; Escherichia coli; Fluoroquinolone; H30; ST131; vagCD