title: Structure Elucidation, Antimicrobial and Cytotoxic Activities of a Halimane Isolated from Vellozia kolbekii Alves (Velloziaceae).
abstract: A new halimane diterpene was isolated from Vellozia kolbekii Alves (Velloziaceae) and identified as (5R,8R,9S,13R)-halim-1,10-ene-15,16-diol (1). It showed cytotoxicity against three human cancer cell lines, SF-295 (glioblastoma), MDA-MB-435 (melanoma), and HCT-8 (colon adenocarcinoma). In the mechanism of cytotoxic action, halimane 1 interferes in two major phases of the cell cycle: in S phase, in which DNA synthesis occurs and where it is very sensitive to damage, and G2M phase which is the phase of preparation for mitosis and mitosis itself, showing apoptosis-inducing properties. Antimicrobial activity towards Gram-positive and Gram-negative bacteria was studied and, against Bacillus cereus, B. subtilis, Escherichia coli, and Pseudomonas aeruginosa, a MIC value of 0.025 μM was observed for halimane 1, which is more active than the positive control chloramphenicol.
authors.affiliation: Institute of Chemistry, Federal University of Rio de Janeiro, Rio de Janeiro, 21941-909, Brazil, (phone: +55 21 39387121; fax: +55 21 39387735).
authors.forename: Carmelita G
authors.initials: CG
authors.lastname: Silva
authors.affiliation: Institute of Chemistry, Federal University of Rio de Janeiro, Rio de Janeiro, 21941-909, Brazil, (phone: +55 21 39387121; fax: +55 21 39387735).
authors.forename: Helvécio M
authors.initials: HM
authors.lastname: Santos Júnior
authors.affiliation: Laboratório de Taxonomia, Bioquímica e Bioprospecção em Fungos FIOCRUZ, Rio de Janeiro 21045-900, Brazil.
authors.forename: Jussara P
authors.initials: JP
authors.lastname: Barbosa
authors.affiliation: Laboratório de Taxonomia, Bioquímica e Bioprospecção em Fungos FIOCRUZ, Rio de Janeiro 21045-900, Brazil.
authors.forename: Gisela L
authors.initials: GL
authors.lastname: Costa
authors.affiliation: Department of Physiology and Pharmacology, School of Medicine, Federal University of Ceará, Ceará 60430-270, Brazil.
authors.forename: Felipe A R
authors.initials: FA
authors.lastname: Rodrigues
authors.affiliation: Departament of Chemistry, Federal University of Lavras, Minas Gerais 37200-000, Brazil.
authors.forename: Denilson F
authors.initials: DF
authors.lastname: Oliveira
authors.affiliation: Department of Physiology and Pharmacology, School of Medicine, Federal University of Ceará, Ceará 60430-270, Brazil.
authors.forename: Letícia V
authors.initials: LV
authors.lastname: Costa-Lotufo
authors.affiliation: Museu Nacional, Federal University of Rio de Janeiro, Rio de Janeiro 20940-040, Brazil.
authors.forename: Ruy J V
authors.initials: RJ
authors.lastname: Alves
authors.affiliation: Institute of Chemistry, Federal University of Rio de Janeiro, Rio de Janeiro, 21941-909, Brazil, (phone: +55 21 39387121; fax: +55 21 39387735).
authors.forename: Elis C A
authors.initials: EC
authors.lastname: Eleutherio
authors.affiliation: Institute of Chemistry, Federal University of Rio de Janeiro, Rio de Janeiro, 21941-909, Brazil, (phone: +55 21 39387121; fax: +55 21 39387735). claudia.rezendeufrj@gmail.com.
authors.forename: Claudia M
authors.initials: CM
authors.lastname: Rezende
mesh_terms: D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects*; D002942:Circular Dichroism; D003603:Cytotoxins / Q000737:chemistry / Q000494:pharmacology; D004224:Diterpenes / Q000737:chemistry*; D006801:Humans; D020128:Inhibitory Concentration 50; D019684:Magnoliopsida / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000890:Anti-Infective Agents; D003603:Cytotoxins; D004224:Diterpenes; D010936:Plant Extracts; C000610653:halimane
keywords: (5R,8R,9S,13R)-Halim-1,10-ene-15,16-diol; Antimicrobial activity; Cytotoxicity; Vellozia kolbekii