title: AcsA-AcsB: The core of the cellulose synthase complex from Gluconacetobacter hansenii ATCC23769.
abstract: The gram-negative bacterium, Gluconacetobacter hansenii, produces cellulose of exceptionally high crystallinity in comparison to the cellulose of higher plants. This bacterial cellulose is synthesized and extruded into the extracellular medium by the cellulose synthase complex (CSC). The catalytic component of this complex is encoded by the gene AcsAB. However, several other genes are known to encode proteins critical to cellulose synthesis and are likely components of the bacterial CSC. We have purified an active heterodimer AcsA-AcsB from G. hansenii ATCC23769 to homogeneity by two different methods. With the purified protein, we have determined how it is post-translationally processed, forming the active heterodimer AcsA-AcsB. Additionally, we have performed steady-state kinetic studies on the AcsA-AcsB complex. Finally through mutagenesis studies, we have explored the roles of the postulated CSC proteins AcsC, AcsD, and CcpAx.
authors.affiliation: Department of Biochemistry and Molecular Biology, 305 South Frear, University Park, The Pennsylvania State University, PA 16802, USA.
authors.forename: John B
authors.initials: JB
authors.lastname: McManus
authors.affiliation: Department of Biochemistry and Molecular Biology, 305 South Frear, University Park, The Pennsylvania State University, PA 16802, USA.
authors.forename: Ying
authors.initials: Y
authors.lastname: Deng
authors.affiliation: Department of Biochemistry and Molecular Biology, 305 South Frear, University Park, The Pennsylvania State University, PA 16802, USA.
authors.forename: Nivedita
authors.initials: N
authors.lastname: Nagachar
authors.affiliation: Department of Biochemistry and Molecular Biology, 305 South Frear, University Park, The Pennsylvania State University, PA 16802, USA.
authors.forename: Teh-Hui
authors.initials: TH
authors.lastname: Kao
authors.affiliation: Department of Biochemistry and Molecular Biology, 305 South Frear, University Park, The Pennsylvania State University, PA 16802, USA. Electronic address: mxt3@psu.edu.
authors.forename: Ming
authors.initials: M
authors.lastname: Tien
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D002384:Catalysis; D020134:Catalytic Domain; D002482:Cellulose / Q000096:biosynthesis; D002498:Centrifugation; D003001:Cloning, Molecular; D019281:Dimerization; D005798:Genes, Bacterial; D041821:Gluconacetobacter / Q000201:enzymology* / Q000235:genetics; D005964:Glucosyltransferases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D007700:Kinetics; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D016254:Mutagenesis, Insertional; D021122:Protein Subunits; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D001426:Bacterial Proteins; D009097:Multienzyme Complexes; D021122:Protein Subunits; D011993:Recombinant Fusion Proteins; D002482:Cellulose; D005964:Glucosyltransferases; C478648:cellulose synthase
keywords: Acetobacter; Cell Wall; Cellulose; Gluconacetobacter hansenii; Glycosyltransferase