title: Assessment of global proteome in LNCaP cells by 2D-RP/RP LC-MS/MS following sulforaphane exposure.
abstract: The phytochemical sulforaphane can induce cell cycle arrest and apoptosis in metastatic prostate cancer cells, though the mechanism of action is not fully known. We conducted a global proteome analysis in LNCaP metastatic prostate cancer cells to characterize how global protein signature responds to sulforaphane. We conducted parallel analyses to evaluate semi-quantitative 1-dimensional versus 2-dimensional liquid chromatography tandem mass spectrometry (LC-MS/MS) and their utility in characterizing whole cell lysate. We show that 2-dimensional LC-MS/MS can be a useful tool for characterizing global protein profiles and identify TRIAP1 as a novel regulator of cell proliferation in LNCaP metastatic prostate cancer cells.
authors.affiliation: Molecular and Cellular Biology, Oregon State University, Corvallis, OR ; Biological and Population Health Sciences, Oregon State University, Corvallis, OR.
authors.forename: Gregory W
authors.initials: GW
authors.lastname: Watson
authors.affiliation: Department of Chemistry, Oregon State University, Corvallis, OR.
authors.forename: Samanthi
authors.initials: S
authors.lastname: Wickramasekara
authors.affiliation: Department of Chemistry, Oregon State University, Corvallis, OR.
authors.forename: Claudia S
authors.initials: CS
authors.lastname: Maier
authors.affiliation: Environmental and Molecular Toxicology, Oregon State University, Corvallis, OR ; Linus Pauling Institute, Oregon State University, Corvallis, OR.
authors.forename: David E
authors.initials: DE
authors.lastname: Williams
authors.affiliation: Center for Epigenetics and Disease Prevention, Institute of Biosciences and Technology, Texas A&M Science Center, Houston, TX ; Department of Nutrition & Food Science, Texas A&M University, College Station, TX ; Department of Clinical Cancer Prevention, MD Anderson Cancer Center, Houston, TX ; Department of Molecular & Cellular Medicine, Texas A&M University College of Medicine, College Station, TX.
authors.forename: Roderick H
authors.initials: RH
authors.lastname: Dashwood
authors.affiliation: Biological and Population Health Sciences, Oregon State University, Corvallis, OR ; Linus Pauling Institute, Oregon State University, Corvallis, OR.
authors.forename: Emily
authors.initials: E
authors.lastname: Ho
keywords: mass spectrometry; prostate cancer; sulforaphane