title: Evaluation of a multiplex OnSpot Primer-Extension PCR assay in the diagnosis of sepsis.
abstract: For evaluation of the Anagnostics Pathogens DNA xA (PxA) assay in the diagnosis of sepsis 58 blood specimens were tested in comparison with the LightCycler SeptiFast assay and blood culture as gold standard. The PxA assay had a lower sensitivity (0.63 vs. 0.8), but higher specificity (0.83 vs. 0.67) than SeptiFast.
authors.affiliation: Division of Hygiene and Medical Microbiology, Medical University Innsbruck, Schöpfstrasse 41, 6020 Innsbruck, Austria. Electronic address: ludwig.knabl@i-med.ac.at.
authors.forename: Ludwig
authors.initials: L
authors.lastname: Knabl
authors.affiliation: Division of Hygiene and Medical Microbiology, Medical University Innsbruck, Schöpfstrasse 41, 6020 Innsbruck, Austria. Electronic address: wolfgang.mutschlechner@i-med.ac.at.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Mutschlechner
authors.affiliation: Division of Hygiene and Medical Microbiology, Medical University Innsbruck, Schöpfstrasse 41, 6020 Innsbruck, Austria.
authors.forename: Dorothea
authors.initials: D
authors.lastname: Orth-Höller
mesh_terms: D016470:Bacteremia / Q000097:blood / Q000175:diagnosis / Q000382:microbiology; D017848:Blood-Borne Pathogens / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000097:blood; D004271:DNA, Fungal / Q000097:blood; D005260:Female; D016469:Fungemia / Q000097:blood / Q000175:diagnosis / Q000382:microbiology; D006801:Humans; D008297:Male; D008875:Middle Aged; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D010865:Pilot Projects; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D018805:Sepsis / Q000097:blood / Q000175:diagnosis* / Q000382:microbiology
chemical_list: D004269:DNA, Bacterial; D004271:DNA, Fungal
keywords: Hybcell; LightCycler SeptiFast; Microarray assay; Multiplex PCR assay; Sepsis diagnostic