title: DNAzyme-Based Logic Gate-Mediated DNA Self-Assembly.
abstract: Controlling DNA self-assembly processes using rationally designed logic gates is a major goal of DNA-based nanotechnology and programming. Such controls could facilitate the hierarchical engineering of complex nanopatterns responding to various molecular triggers or inputs. Here, we demonstrate the use of a series of DNAzyme-based logic gates to control DNA tile self-assembly onto a prescribed DNA origami frame. Logic systems such as "YES," "OR," "AND," and "logic switch" are implemented based on DNAzyme-mediated tile recognition with the DNA origami frame. DNAzyme is designed to play two roles: (1) as an intermediate messenger to motivate downstream reactions and (2) as a final trigger to report fluorescent signals, enabling information relay between the DNA origami-framed tile assembly and fluorescent signaling. The results of this study demonstrate the plausibility of DNAzyme-mediated hierarchical self-assembly and provide new tools for generating dynamic and responsive self-assembly systems.
authors.affiliation: Institute of Software, School of Electronics Engineering and Computer Science, Peking University , Beijing, China.|School of Molecular Sciences, Center for Molecule Design and Biomimetics at the Biodesign Institute, Arizona State University , Tempe, Arizona 85281, United States.
authors.forename: Cheng
authors.initials: C
authors.lastname: Zhang
authors.affiliation: School of Control and Computer Engineering, North China Electric Power University , Beijing, China.|School of Molecular Sciences, Center for Molecule Design and Biomimetics at the Biodesign Institute, Arizona State University , Tempe, Arizona 85281, United States.
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.affiliation: School of Molecular Sciences, Center for Molecule Design and Biomimetics at the Biodesign Institute, Arizona State University , Tempe, Arizona 85281, United States.
authors.forename: Shuoxing
authors.initials: S
authors.lastname: Jiang
authors.affiliation: School of Molecular Sciences, Center for Molecule Design and Biomimetics at the Biodesign Institute, Arizona State University , Tempe, Arizona 85281, United States.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: School of Molecular Sciences, Center for Molecule Design and Biomimetics at the Biodesign Institute, Arizona State University , Tempe, Arizona 85281, United States.
authors.forename: Hao
authors.initials: H
authors.lastname: Yan
mesh_terms: D039301:Computers, Molecular; D004247:DNA / Q000737:chemistry*; D021881:DNA, Catalytic / Q000737:chemistry*; D008128:Logic; D036103:Nanotechnology
chemical_list: D021881:DNA, Catalytic; D004247:DNA
keywords: DNA origami; DNA self-assembly; DNAzyme; logic gates