title: Electrogenic transport and K(+) ion channel expression by the human endolymphatic sac epithelium.
abstract: The endolymphatic sac (ES) is a cystic organ that is a part of the inner ear and is connected to the cochlea and vestibule. The ES is thought to be involved in inner ear ion homeostasis and fluid volume regulation for the maintenance of hearing and balance function. Many ion channels, transporters, and exchangers have been identified in the ES luminal epithelium, mainly in animal studies, but there has been no functional study investigating ion transport using human ES tissue. We designed the first functional experiments on electrogenic transport in human ES and investigated the contribution of K(+) channels in the electrogenic transport, which has been rarely identified, even in animal studies, using electrophysiological/pharmacological and molecular biological methods. As a result, we identified functional and molecular evidence for the essential participation of K(+) channels in the electrogenic transport of human ES epithelium. The identified K(+) channels involved in the electrogenic transport were KCNN2, KCNJ14, KCNK2, and KCNK6, and the K(+) transports via those channels are thought to play an important role in the maintenance of the unique ionic milieu of the inner ear fluid.
authors.affiliation: Department of Otorhinolaryngology, Yonsei University College of Medicine, 50-1 Yonsei-Ro, Seodaemun-Gu, Seoul, 03722, Republic of Korea.|The Airway Mucus Institute, Yonsei University College of Medicine, 50-1 Yonsei-Ro, Seodaemun-Gu, Seoul, 03722, Republic of Korea.
authors.forename: Sung Huhn
authors.initials: SH
authors.lastname: Kim
authors.affiliation: Department of Otorhinolaryngology, Sooncheonhyang University, College of Medicine, 170 Jomaru-ro, Wonmi-Gu, Bucheon City, Gyenggi-Do, 14584, Republic of Korea.
authors.forename: Bo Gyung
authors.initials: BG
authors.lastname: Kim
authors.affiliation: The Airway Mucus Institute, Yonsei University College of Medicine, 50-1 Yonsei-Ro, Seodaemun-Gu, Seoul, 03722, Republic of Korea.|Research Center for the Human Natural Defense System, Yonsei University College of Medicine, 50-1 Yonsei-Ro, Seodaemun-Gu, Seoul, 03722, Republic of Korea.
authors.forename: Jin Young
authors.initials: JY
authors.lastname: Kim
authors.affiliation: Department of Otorhinolaryngology, Yonsei University College of Medicine, 50-1 Yonsei-Ro, Seodaemun-Gu, Seoul, 03722, Republic of Korea.
authors.forename: Kyung Jin
authors.initials: KJ
authors.lastname: Roh
authors.affiliation: Department of Otorhinolaryngology, Yonsei University College of Medicine, 50-1 Yonsei-Ro, Seodaemun-Gu, Seoul, 03722, Republic of Korea.
authors.forename: Michelle J
authors.initials: MJ
authors.lastname: Suh
authors.affiliation: Department of Otorhinolaryngology, Yonsei University College of Medicine, 50-1 Yonsei-Ro, Seodaemun-Gu, Seoul, 03722, Republic of Korea.
authors.forename: JinSei
authors.initials: J
authors.lastname: Jung
authors.affiliation: Department of Otorhinolaryngology, Yonsei University College of Medicine, 50-1 Yonsei-Ro, Seodaemun-Gu, Seoul, 03722, Republic of Korea.
authors.forename: In Seok
authors.initials: IS
authors.lastname: Moon
authors.affiliation: Pathogenesis of Ear Diseases Laboratory, Department of Head and Neck Surgery, David Geffen School of Medicine, University of California Los Angeles, 2100 W. Third St, Los Angeles, CA, 90057, USA.
authors.forename: Sung K
authors.initials: SK
authors.lastname: Moon
authors.affiliation: Department of Otorhinolaryngology, Yonsei University College of Medicine, 50-1 Yonsei-Ro, Seodaemun-Gu, Seoul, 03722, Republic of Korea.|The Airway Mucus Institute, Yonsei University College of Medicine, 50-1 Yonsei-Ro, Seodaemun-Gu, Seoul, 03722, Republic of Korea.
authors.forename: Jae Young
authors.initials: JY
authors.lastname: Choi
mesh_terms: D002853:Chromatography, Liquid; D003051:Cochlea / Q000378:metabolism / Q000502:physiology; D055724:Electrophysiological Phenomena; D004712:Endolymphatic Sac / Q000378:metabolism / Q000502:physiology*; D004848:Epithelium / Q000378:metabolism / Q000502:physiology*; D005260:Female; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D017136:Ion Transport / Q000502:physiology; D008297:Male; D008875:Middle Aged; D011188:Potassium / Q000378:metabolism*; D015221:Potassium Channels / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D024683:Potassium Channels, Tandem Pore Domain / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051640:Small-Conductance Calcium-Activated Potassium Channels / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D053719:Tandem Mass Spectrometry
chemical_list: C118290:KCNK6 protein, human; C496897:KCNN2 protein, human; D015221:Potassium Channels; D024683:Potassium Channels, Tandem Pore Domain; D051640:Small-Conductance Calcium-Activated Potassium Channels; C103742:potassium channel protein TREK-1; D011188:Potassium