title: Simultaneous Determination of Eight Hypotensive Drugs of Various Chemical Groups in Pharmaceutical Preparations by HPLC-DAD.
abstract: A new sensitive, simple, rapid, and precise HPLC method with diode array detection has been developed for separation and simultaneous determination of hydrochlorothiazide, furosemide, torasemide, losartane, quinapril, valsartan, spironolactone, and canrenone in combined pharmaceutical dosage forms. The chromatographic analysis of the tested drugs was performed on an ACE C18, 100 Å, 250×4.6 mm, 5 μm particle size column with 0.0.05 M phosphate buffer (pH=3.00)-acetonitrile-methanol (30+20+50 v/v/v) mobile phase at a flow rate of 1.0 mL/min. The column was thermostatted at 25°C. UV detection was performed at 230 nm. Analysis time was 10 min. The elaborated method meets the acceptance criteria for specificity, linearity, sensitivity, accuracy, and precision. The proposed method was successfully applied for the determination of the studied drugs in the selected combined dosage forms.
authors.affiliation: Jagiellonian University Medical College, Faculty of Pharmacy, Department of Inorganic and Analytical Chemistry, 9 Medyczna St, 30-688 Kraków, Poland.
authors.forename: Mariusz
authors.initials: M
authors.lastname: Stolarczyk
authors.forename: Urszula
authors.initials: U
authors.lastname: Hubicka
authors.forename: Barbara
authors.initials: B
authors.lastname: Żuromska-Witek
authors.forename: Jan
authors.initials: J
authors.lastname: Krzek
mesh_terms: D000959:Antihypertensive Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D057230:Limit of Detection
chemical_list: D000959:Antihypertensive Agents