title: Root Glucosinolate Profiles for Screening of Radish (Raphanus sativus L.) Genetic Resources.
abstract: Radish (Raphanus sativus L.), a root vegetable, is rich in glucosinolates (GLs), which are beneficial secondary metabolites for human health. To investigate the genetic variations in GL content in radish roots and the relationship with other root phenotypes, we analyzed 71 accessions from 23 different countries for GLs using HPLC. The most abundant GL in radish roots was glucoraphasatin, a GL with four-carbon aliphatic side chain. The content of glucoraphasatin represented at least 84.5% of the total GL content. Indolyl GL represented only 3.1% of the total GL at its maximum. The principal component analysis of GL profiles with various root phenotypes showed that four different genotypes exist in the 71 accessions. Although no strong correlation with GL content and root phenotype was observed, the varied GL content levels demonstrate the genetic diversity of GL content, and the amount that GLs could be potentially improved by breeding in radishes.
authors.forename: Gibum
authors.initials: G
authors.lastname: Yi
authors.forename: Sooyeon
authors.initials: S
authors.lastname: Lim
authors.affiliation: Department of Horticultural Crop Research, National Institute of Horticultural and Herbal Science, Rural Development Administration , Wanju-gun, Jeollabuk-do 55365, Korea.
authors.forename: Won Byoung
authors.initials: WB
authors.lastname: Chae
authors.forename: Jeong Eun
authors.initials: JE
authors.lastname: Park
authors.forename: Hye Rang
authors.initials: HR
authors.lastname: Park
authors.forename: Eun Jin
authors.initials: EJ
authors.lastname: Lee
authors.forename: Jin Hoe
authors.initials: JH
authors.lastname: Huh
mesh_terms: D002851:Chromatography, High Pressure Liquid; D005961:Glucosinolates / Q000737:chemistry*; D006801:Humans; D010936:Plant Extracts / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry / Q000145:classification / Q000235:genetics; D031224:Raphanus / Q000737:chemistry* / Q000145:classification / Q000235:genetics; D014675:Vegetables / Q000737:chemistry* / Q000145:classification / Q000235:genetics
chemical_list: D005961:Glucosinolates; D010936:Plant Extracts
keywords: Raphanus sativus; glucoraphasatin; glucosinolates; radish; secondary metabolite