title: lncRNA DANCR suppresses odontoblast-like differentiation of human dental pulp cells by inhibiting wnt/β-catenin pathway.
abstract: Long noncoding RNAs (lncRNAs) have recently emerged as an important class of regulatory molecules in diverse biological processes, although lncRNA involvement in the odontoblast-like differentiation of human dental pulp cells (hDPCs) is poorly understood. We investigate the expression of lncRNAs in this differentiation and explore their underlying role and the involved mechanism. Integrated comparative lncRNA microarray profiling was used to examine lncRNA expression during this differentiation. The differential expression of lncRNAs was validated by quantitative real-time reverse transcription plus the polymerase chain reaction. Differential lncRNA overexpression was performed with an adenoviral vector and the role and mechanism was then investigated in odontoblast-like differentiation. We identified 139 differentially expressed lncRNAs during this differentiation. Among them, five lncRNAs differentially expressed in microarray analysis were validated. Notably, lncRNA DANCR expression was significantly downregulated during hDPC differentiation to odontoblast-like cells in a time-dependent manner. Moreover, lncRNA DANCR overexpression blocked mineralized nodule formation and the expression of DSPP and DMP-1 in hDPCs after 14 days of odontogenic induction. Importantly, the upregulation of DANCR significantly decreased the expression levels of p-GSK-3β and β-catenin expression indicating that lncRNA DANCR can inhibit the activation of the Wnt/β-catenin signal pathway during the odontoblast-like differentiation of hDPCs. Thus, the modulation of Wnt/β-catenin signaling by lncRNA DANCR represents a potential therapeutic option for reparative dentin formation and regenerative endodontics.
authors.affiliation: Department of Operative Dentistry and Endodontics, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University and Guangdong Provincial Key Laboratory of Stomatology, Guangzhou, Guangdong, 510055, People's Republic of China.
authors.forename: Lingling
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Operative Dentistry and Endodontics, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University and Guangdong Provincial Key Laboratory of Stomatology, Guangzhou, Guangdong, 510055, People's Republic of China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Song
authors.affiliation: Department of Operative Dentistry and Endodontics, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University and Guangdong Provincial Key Laboratory of Stomatology, Guangzhou, Guangdong, 510055, People's Republic of China.
authors.forename: Shuheng
authors.initials: S
authors.lastname: Huang
authors.affiliation: Department of Operative Dentistry and Endodontics, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University and Guangdong Provincial Key Laboratory of Stomatology, Guangzhou, Guangdong, 510055, People's Republic of China.
authors.forename: Runfu
authors.initials: R
authors.lastname: Wang
authors.affiliation: Department of Operative Dentistry and Endodontics, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University and Guangdong Provincial Key Laboratory of Stomatology, Guangzhou, Guangdong, 510055, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Qin
authors.affiliation: Department of Operative Dentistry and Endodontics, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University and Guangdong Provincial Key Laboratory of Stomatology, Guangzhou, Guangdong, 510055, People's Republic of China.
authors.forename: Jia
authors.initials: J
authors.lastname: Guo
authors.affiliation: Department of Operative Dentistry and Endodontics, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University and Guangdong Provincial Key Laboratory of Stomatology, Guangzhou, Guangdong, 510055, People's Republic of China. linzhm@mail.sysu.edu.cn.
authors.forename: Zhengmei
authors.initials: Z
authors.lastname: Lin
mesh_terms: D002454:Cell Differentiation / Q000235:genetics*; D002478:Cells, Cultured; D003782:Dental Pulp / Q000166:cytology*; D003804:Dentin / Q000378:metabolism; D000071679:Glycogen Synthase Kinase 3 beta / Q000096:biosynthesis; D006801:Humans; D009804:Odontoblasts / Q000166:cytology*; D009805:Odontogenesis / Q000235:genetics*; D062085:RNA, Long Noncoding / Q000235:genetics*; D051153:Wnt Proteins / Q000037:antagonists & inhibitors*; D060449:Wnt Signaling Pathway / Q000235:genetics*; D051176:beta Catenin / Q000037:antagonists & inhibitors* / Q000096:biosynthesis
chemical_list: C000625902:DANCR long noncoding RNA, human; D062085:RNA, Long Noncoding; D051153:Wnt Proteins; D051176:beta Catenin; D000071679:Glycogen Synthase Kinase 3 beta
keywords: Cell differentiation; Long noncoding RNA; Odontoblasts; Odontogenesis; Wnt signaling pathway