title: Fluorescence In Situ Hybridization on Rice Chromosomes.
abstract: Fluorescence in situ hybridization (FISH) has become one of the most important technologies applied in plant molecular cytogenetic research. FISH technique has been not only well applied in physical mapping and genomic studies, but also served as an indispensable tool in tracing the individual chromosome during cell division. This chapter provides protocols for basic FISH analysis using rice as a model, which can also be adapted to other model plant species.
authors.affiliation: State Key Laboratory of Plant Genomics and Center for Plant Gene Research, Chinese Academy of Sciences, Institute of Genetics and Developmental Biology, No 1 West Beichen Road, Chaoyang District, Beijing, 100101, China.
authors.forename: Yafei
authors.initials: Y
authors.lastname: Li
authors.affiliation: State Key Laboratory of Plant Genomics and Center for Plant Gene Research, Chinese Academy of Sciences, Institute of Genetics and Developmental Biology, No 1 West Beichen Road, Chaoyang District, Beijing, 100101, China. zkcheng@genetics.ac.cn.
authors.forename: Zhukuan
authors.initials: Z
authors.lastname: Cheng
mesh_terms: D032461:Chromosomes, Plant; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008540:Meiosis; D008938:Mitosis; D012275:Oryza / Q000166:cytology* / Q000235:genetics*
keywords: Chromosome; FISH; Meiosis; Mitosis; Rice