title: Characterization and Quantitation of Triterpenoid Saponins in Raw and Sprouted Chenopodium berlandieri spp. (Huauzontle) Grains Subjected to Germination with or without Selenium Stress Conditions.
abstract: Pseudocereal Chenopodium berlandieri spp. (huauzontle) was evaluated to determine saponin composition. Saponins were evaluated in raw and germinated grains subjected to chemical stress induced by sodium selenite. Analysis by liquid chromatography coupled with ELSD detector revealed the presence of 12 saponins, identified according to compounds previously assayed in Chenopodium quinoa. Saponins found at the highest concentrations in raw grains were derived from oleanolic and phytolaccagenic acids. Total saponin concentration significantly decreased in germinated compared to raw grains due to the significant loss of 90.1% and 95.7% of the phytolaccagenic acid without and with chemical selenium stress, respectively. The most abundant saponin in germinated sprouts decreased during normal germination. Interestingly, the concentration of this particular saponin significantly increased during the Se-induced stress germination. Chemical stress with selenium salts proved to change the saponin composition in geminated Chenopodium berlandieri spp. grains, therefore affecting their potential use as ingredient in the food industry.
authors.affiliation: Centro de Biotecnología-FEMSA, Escuela de Biotecnología y Alimentos, Tecnológico de Monterrey-Campus Monterrey, Av. Eugenio Garza Sada 2501 Sur, C.P. 64849, Monterrey, N.L., México.
authors.forename: Marco A
authors.initials: MA
authors.lastname: Lazo-Vélez
authors.affiliation: Centro de Biotecnología-FEMSA, Escuela de Biotecnología y Alimentos, Tecnológico de Monterrey-Campus Monterrey, Av. Eugenio Garza Sada 2501 Sur, C.P. 64849, Monterrey, N.L., México.
authors.forename: Daniel
authors.initials: D
authors.lastname: Guajardo-Flores
authors.affiliation: Centro de Biotecnología-FEMSA, Escuela de Biotecnología y Alimentos, Tecnológico de Monterrey-Campus Monterrey, Av. Eugenio Garza Sada 2501 Sur, C.P. 64849, Monterrey, N.L., México.
authors.forename: Daniel
authors.initials: D
authors.lastname: Mata-Ramírez
authors.affiliation: Centro de Biotecnología-FEMSA, Escuela de Biotecnología y Alimentos, Tecnológico de Monterrey-Campus Monterrey, Av. Eugenio Garza Sada 2501 Sur, C.P. 64849, Monterrey, N.L., México.
authors.forename: Janet A
authors.initials: JA
authors.lastname: Gutiérrez-Uribe
authors.affiliation: Centro de Biotecnología-FEMSA, Escuela de Biotecnología y Alimentos, Tecnológico de Monterrey-Campus Monterrey, Av. Eugenio Garza Sada 2501 Sur, C.P. 64849, Monterrey, N.L., México.
authors.forename: Sergio O
authors.initials: SO
authors.lastname: Serna-Saldivar
mesh_terms: D027462:Chenopodium / Q000737:chemistry* / Q000187:drug effects / Q000254:growth & development; D018525:Germination; D012503:Saponins / Q000032:analysis*; D012639:Seeds / Q000737:chemistry / Q000187:drug effects / Q000254:growth & development; D012643:Selenium / Q000032:analysis / Q000494:pharmacology*; D014315:Triterpenes / Q000032:analysis*
chemical_list: D012503:Saponins; D014315:Triterpenes; D012643:Selenium
keywords: Chenopodium berlandieri spp; HPLC-MS; germination; saponin; selenium