title: A tool for the selective sequestration of ATP and PPi to aid in-solution phosphopeptide detection assays.
abstract: The presence of small phospho-anions, such as PPi and ATP in protein samples often complicates the robust detection of phosphoproteins by metal-based chemosensors and receptors. We herein report the development of a bis(Zn(2+)-cyclen)-triethylbenzene scaffold which can selectively sequester PPi and ATP without affecting the detection of a di-phosphorylated peptide by a ProxyPhos chemosensor.
authors.affiliation: Department of Chemistry and Department of Chemical and Physical Sciences, University of Toronto, Mississauga, 3359 Mississauga Road North, Mississauga, Ontario, CanadaL5L1C6. patrick.gunning@utoronto.ca.
authors.forename: Eugenia
authors.initials: E
authors.lastname: Duodu
authors.forename: Dziyana
authors.initials: D
authors.lastname: Kraskouskaya
authors.forename: Rodolfo F
authors.initials: RF
authors.lastname: Gómez-Biagi
authors.forename: Patrick T
authors.initials: PT
authors.lastname: Gunning
mesh_terms: D000255:Adenosine Triphosphate / Q000737:chemistry*; D001555:Benzene Derivatives / Q000737:chemistry; D056831:Coordination Complexes / Q000737:chemistry*; D011756:Diphosphates / Q000737:chemistry*; D047028:Macrocyclic Compounds / Q000737:chemistry; D010748:Phosphopeptides / Q000032:analysis*; D015032:Zinc / Q000737:chemistry
chemical_list: D001555:Benzene Derivatives; D056831:Coordination Complexes; D011756:Diphosphates; D047028:Macrocyclic Compounds; D010748:Phosphopeptides; C107241:diphosphoric acid; D000255:Adenosine Triphosphate; D015032:Zinc