title: No association of IL-12p40 pro1.1 polymorphism with juvenile idiopathic arthritis.
abstract: BACKGROUND
  IL-12p40 plays an important role in the activation of the T-cell lines like Th17 and Th1-cells. Theses cells are crucial in the pathogenesis of juvenile idiopathic arthritis. A polymorphism in its promoter region and the genotype IL12p40 pro1.1 leads to a higher production of IL-12p40. We studied whether there is a difference in the distribution of the genotype in patients with JIA and the healthy population.
  
  
  METHODS
  In 883 patients and 321 healthy controls the IL-12p40 promoter genotype was identified by ARMS-PCR.
  
  
  RESULTS
  There is no association of IL-12p40 pro polymorphism neither in patients with JIA compared to controls nor in subtypes of JIA compared to oligoarthritis. We found a non-significant tendency of a higher prevalence of the genotype pro1.1 in systemic arthritis (32.4%) and in rheumatoid factor negative polyarthritis (30.5%) and a lower pro1.1 genotype in persistent oligoarthritis (20.7%) and in enthesitis-related arthritis (17%). Likelihood of the occurrence of genotype IL12-p40 pro1.1 in patients with systemic arthritis (OR 1.722, CI 95% 1.344-2.615, p 0.0129) and RF-negative polyarthritis (OR 1.576, CI 95% 1.046-2.376, p 0.0367) compared to persistent oligoarthritis was significantly higher. This was also true for comparison of their homozygous genotypes IL-12p40 pro 1.1 and 2.2 in systemic arthritis (OR 1.779, CI 95 % 1.045-3.029, p 0.0338). However, in Bonferroni correction for multiple hypothesis this was not significant.
  
  
  CONCLUSIONS
  A tendency of a higher prevalence of the genotype IL-12p40 pro1.1 in systemic arthritis and in rheumatoid factor negative polyarthritis was observed but not significant. Further investigations should be done to clarify the role IL-12p40 in the different subtypes of JIA.
authors.affiliation: Département de l'enfant et de l'adolescent, Hôpitaux Universitaires de Genève, Genève, Switzerland. christiane.eberhardt@gmail.com.
authors.forename: Christiane S
authors.initials: CS
authors.lastname: Eberhardt
authors.affiliation: Deutsches Zentrum für Kinder- und Jugendrheumatologie, Garmisch-Partenkirchen, Germany. Haas.Johannes-Peter@rheuma-kinderklinik.de.
authors.forename: Johannes-Peter
authors.initials: JP
authors.lastname: Haas
authors.affiliation: Klinik für Kinder- und Jugendmedizin, Vivantes Klinikum im Friedrichshain, Berlin, Germany. Hermann.Girschick@vivantes.de.
authors.forename: Hermann
authors.initials: H
authors.lastname: Girschick
authors.affiliation: Kinderklinik der Bayerischen Julius-Maximilians-Universität, Universitätsklinikum, Würzburg, Germany. Schwarz@st-josef-stift.de.
authors.forename: Tobias
authors.initials: T
authors.lastname: Schwarz
authors.affiliation: Kinderklinik der Bayerischen Julius-Maximilians-Universität, Universitätsklinikum, Würzburg, Germany. Morbach_H@kinderklinik.uni-wuerzburg.de.
authors.forename: Henner
authors.initials: H
authors.lastname: Morbach
authors.affiliation: Klinik für Kinder- und Jugendmedizin, Universitätsklinikum Carl Gustav Carus, Dresden, Germany. Angela.Roesen-Wolff@uniklinikum-dresden.de.
authors.forename: Angela
authors.initials: A
authors.lastname: Rösen-Wolff
authors.affiliation: Institut für Immunologie, Universität Münster, Münster, Germany. dfoell@uni-muenster.de.
authors.forename: Dirk
authors.initials: D
authors.lastname: Foell
authors.affiliation: Pädiatrie 5, Olgahospital Stuttgart, Stuttgart, Germany. guenther.dannecker@gmx.de.
authors.forename: Guenther
authors.initials: G
authors.lastname: Dannecker
authors.affiliation: Klinik für Anästhesiologie, Klinikum der Universität Regensburg, Regensburg, Germany. Carsten.Schepp@gmx.de.
authors.forename: Carsten
authors.initials: C
authors.lastname: Schepp
authors.affiliation: Klinik für Kinder-und Jugendrheumatologie, St Josef Stift Sendenhorst, Sendenhorst, Germany. ganser@st-josef-stift.de.
authors.forename: Gerd
authors.initials: G
authors.lastname: Ganser
authors.affiliation: Department of Pediatrics, Division of Pediatric Pneumology, Allergology and Immunology, RWTH Aachen, University, Pauwelsstr 30, D-52074, Aachen, Germany. nhonke@ukaachen.de.
authors.forename: Nora
authors.initials: N
authors.lastname: Honke
authors.affiliation: Institut für Humangenetik, Heidelberg, Germany. theggermann@ukaachen.de.
authors.forename: Thomas
authors.initials: T
authors.lastname: Eggermann
authors.affiliation: Paul-Ehrlich Institut, Langen, Germany. jan.muellerberghaus@gmail.com.
authors.forename: Jan
authors.initials: J
authors.lastname: Müller-Berghaus
authors.affiliation: Department of Pediatrics, Division of Pediatric Pneumology, Allergology and Immunology, RWTH Aachen, University, Pauwelsstr 30, D-52074, Aachen, Germany. nwagner@ukaachen.de.
authors.forename: Norbert
authors.initials: N
authors.lastname: Wagner
authors.affiliation: Department of Pediatrics, Division of Pediatric Pneumology, Allergology and Immunology, RWTH Aachen, University, Pauwelsstr 30, D-52074, Aachen, Germany. kohl@ukaachen.de.
authors.forename: Kim
authors.initials: K
authors.lastname: Ohl
authors.affiliation: Department of Pediatrics, Division of Pediatric Pneumology, Allergology and Immunology, RWTH Aachen, University, Pauwelsstr 30, D-52074, Aachen, Germany. ktenbrock@ukaachen.de.
authors.forename: Klaus
authors.initials: K
authors.lastname: Tenbrock
mesh_terms: D001171:Arthritis, Juvenile / Q000235:genetics*; D016022:Case-Control Studies; D006801:Humans; D053762:Interleukin-12 Subunit p40 / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics
chemical_list: D053762:Interleukin-12 Subunit p40