title: Improvement in the docosahexaenoic acid production of Schizochytrium sp. S056 by replacement of sea salt.
abstract: Schizochytrium is a marine microalga that requires high concentrations of sea salt for growth, although problems arise with significant amounts of chloride ions in the culture medium, which corrodes the fermenters. In this work, we evaluated that cell growth and docosahexaenoic acid (DHA) production can be improved when using 1 % (w/v) sodium sulfate instead of 2 % (w/v) sea salt in the culture medium for Schizochytrium sp. S056. In practice, the use of sodium sulfate as the sodium salt led to chloride ion levels in the medium that can be completely removed, thus avoiding fermenter corrosion during Schizochytrium sp. S056 growth, reducing cost and increasing DHA production, and simplifying the disposal of fermentation wastewater. Additionally, we demonstrated that the osmolality of growth media did not play a crucial role in the production of DHA. These findings may be significantly important to companies involved in production of PUFAs by marine microbes.
authors.affiliation: Department of Biotechnology, Institute of Resource Biology and Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, No. 1037 Luoyu Road, Wuhan, 430074, China.|Key Laboratory of Molecular Biophysics, Ministry of Education, Wuhan, 430074, China.|Wuhan Institute of Biotechnology, Wuhan, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.affiliation: Department of Biotechnology, Institute of Resource Biology and Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, No. 1037 Luoyu Road, Wuhan, 430074, China.|Key Laboratory of Molecular Biophysics, Ministry of Education, Wuhan, 430074, China.|Wuhan Institute of Biotechnology, Wuhan, China.
authors.forename: Pengpeng
authors.initials: P
authors.lastname: Zhou
authors.affiliation: Department of Biotechnology, Institute of Resource Biology and Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, No. 1037 Luoyu Road, Wuhan, 430074, China.|Key Laboratory of Molecular Biophysics, Ministry of Education, Wuhan, 430074, China.|Wuhan Institute of Biotechnology, Wuhan, China.
authors.forename: Yuanmin
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Department of Biotechnology, Institute of Resource Biology and Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, No. 1037 Luoyu Road, Wuhan, 430074, China.|Key Laboratory of Molecular Biophysics, Ministry of Education, Wuhan, 430074, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Xie
authors.affiliation: Department of Biotechnology, Institute of Resource Biology and Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, No. 1037 Luoyu Road, Wuhan, 430074, China.|Key Laboratory of Molecular Biophysics, Ministry of Education, Wuhan, 430074, China.|Wuhan Institute of Biotechnology, Wuhan, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Ma
authors.affiliation: Department of Biotechnology, Institute of Resource Biology and Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, No. 1037 Luoyu Road, Wuhan, 430074, China.|Key Laboratory of Molecular Biophysics, Ministry of Education, Wuhan, 430074, China.|Wuhan Institute of Biotechnology, Wuhan, China.
authors.forename: Xiaopeng
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Biotechnology, Institute of Resource Biology and Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, No. 1037 Luoyu Road, Wuhan, 430074, China.
authors.forename: Zhendong
authors.initials: Z
authors.lastname: Bao
authors.affiliation: Department of Biotechnology, Institute of Resource Biology and Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, No. 1037 Luoyu Road, Wuhan, 430074, China. yulongjiang@hust.edu.cn.|Key Laboratory of Molecular Biophysics, Ministry of Education, Wuhan, 430074, China. yulongjiang@hust.edu.cn.|Wuhan Institute of Biotechnology, Wuhan, China. yulongjiang@hust.edu.cn.
authors.forename: Longjiang
authors.initials: L
authors.lastname: Yu
mesh_terms: D004281:Docosahexaenoic Acids / Q000096:biosynthesis*; D058086:Microalgae / Q000254:growth & development*; D012965:Sodium Chloride / Q000494:pharmacology*; D058009:Stramenopiles / Q000254:growth & development*; D013431:Sulfates / Q000494:pharmacology*
chemical_list: D013431:Sulfates; C012036:sodium sulfate; D004281:Docosahexaenoic Acids; D012965:Sodium Chloride
keywords: Docosahexaenoic acid; Marine microalgae; Osmolality; Schizochytrium; Sea salt; Sodium sulfate