title: ENDOTHELIAL PROGENITOR CELL ADHESION, GROWTH AND CHARACTERIZATION ON TRABECULAR TITANIUM AND TRABECULAR TITANIUM COATED WITH COLLAGEN OR DECELLULARIZED ECM.
abstract: Adequate blood supply is essential for prosthesis osteointegration and bone healing as it supplies oxygen, nutrition and progenitor cells. The bone healing process and vascularization depend upon the endothelial cells, which speed up implant osteointegration. Endothelial Progenitor Cells (EPC) are a population of stem cells that can reproduce, migrate and acquire mature endothelial phenotype. Their recruitment occurs in the tissue lesion to enhance neovascularization. Trabecular TitaniumTM (TTTM) is a new biomaterial with very interesting biomechanical characteristics and fast osteointegration. This study has investigated adhesion, proliferation and characteristics of EPC on three types of biomaterial: unmodified trabecular titanium, trabecular titanium coated with the ECM deposited by human mesenchymal stem cells isolated from subcutaneous adipose tissue and decellularized and trabecular titanium coated with type I collagen (control scaffold). MTT assay showed similar percentages of EPCs seeded on the different kinds of scaffold: 67% on TT, 70% on decellularized scaffolds and 82% on collagen-coated scaffolds. There were no statistically significant differences between the three groups. We therefore conclude that TTTM allows EPC adhesion and proliferation and, consequently, by permitting vascularization, it favours prosthesis osteointegration.
authors.affiliation: Department of Molecular Medicine, Human Physiology Unit, University of Pavia, Italy; Centre for Health Technologies (CHT), University of Pavia, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Gastaldi
authors.affiliation: Department of Clinical-Surgical Sciences, Diagnostics and Pediatrics, University of Pavia, Operative Unit of Orthopaedics and Traumatology, Fondazione IRCCS Policlinico San Matteo, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Caliogna
authors.affiliation: Department of Biology and Biotechnology Lazzaro Spallanzani, University of Pavia, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Botta
authors.affiliation: Department of Clinical-Surgical Sciences, Diagnostics and Pediatrics, University of Pavia, Operative Unit of Orthopaedics and Traumatology, Fondazione IRCCS Policlinico San Matteo, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Ghiara
authors.affiliation: Department of Clinical-Surgical Sciences, Diagnostics and Pediatrics, University of Pavia, Operative Unit of Orthopaedics and Traumatology, Fondazione IRCCS Policlinico San Matteo, Italy; Centre for Health Technologies (CHT), University of Pavia, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Benazzo