title: Thrombin-mediated ratiometric two-photon fluorescent probe for selective imaging of endogenous ultratrace glutathione in platelet.
abstract: Ultratrace change of reduced glutathione (GSH) can weaken coagulation function of platelet (PLT). Thus, rapid and sensitive imaging of GSH specific in PLT is beneficial for monitoring coagulation function of PLT. Many fluorescent probes for GSH have been reported, but ratio fluorescent probe with excellent two-photon property for screening PLT from peripheral blood and quantitative imaging of GSH are scarce. In this work, a thrombin-mediated two-photon GSH-specific fluorescent probe (IQDC-L) was reported. Sulfuric diamide, a key group as linker, was introduced into IQDC-L, which resulted in not only specific selectivity for GSH, but also FRET occurring in probe. When IQDC-L encountered GSH, "S-N" in sulfonamide group was cut off, and FRET was inhibited. Furthermore, fluorescence intensities at 520 and 595 nm presented linear change on ratio mode in the range of GSH (2.0-65 nM). The lowest detection for GSH was as low as 0.083 nM. Intriguingly, IQDC-L under thrombin-mediated was able to screen PLT from peripheral blood without any interference. Thus, IQDC-L could be used to screen PLT from peripheral blood, and simultaneously, to in situ image ultratrace GSH.
authors.affiliation: Collaborative Innovation Center of Henan Province for Green Manufacturing of Fine Chemicals, Key Laboratory of Green Chemical Media and Reactions, Ministry of Education, Henan Normal University, Xinxiang, Henan 453007, PR China; Henan Key Laboratory of Green Chemical Media and Reactions, School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang, Henan 453007, PR China. Electronic address: zhanghua1106@163.com.
authors.forename: Hua
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Collaborative Innovation Center of Henan Province for Green Manufacturing of Fine Chemicals, Key Laboratory of Green Chemical Media and Reactions, Ministry of Education, Henan Normal University, Xinxiang, Henan 453007, PR China; Henan Key Laboratory of Green Chemical Media and Reactions, School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang, Henan 453007, PR China.
authors.forename: Caixia
authors.initials: C
authors.lastname: Wang
authors.affiliation: Xinxiang Medical University, 601 Jinsui Road, Hongqi Zone, Xinxiang 453000, PR China.
authors.forename: Ge
authors.initials: G
authors.lastname: Wang
authors.affiliation: Collaborative Innovation Center of Henan Province for Green Manufacturing of Fine Chemicals, Key Laboratory of Green Chemical Media and Reactions, Ministry of Education, Henan Normal University, Xinxiang, Henan 453007, PR China; Henan Key Laboratory of Green Chemical Media and Reactions, School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang, Henan 453007, PR China.
authors.forename: Kui
authors.initials: K
authors.lastname: Wang
authors.affiliation: Collaborative Innovation Center of Henan Province for Green Manufacturing of Fine Chemicals, Key Laboratory of Green Chemical Media and Reactions, Ministry of Education, Henan Normal University, Xinxiang, Henan 453007, PR China; Henan Key Laboratory of Green Chemical Media and Reactions, School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang, Henan 453007, PR China. Electronic address: jiangkai6898@163.com.
authors.forename: Kai
authors.initials: K
authors.lastname: Jiang
mesh_terms: D015374:Biosensing Techniques; D001792:Blood Platelets / Q000737:chemistry*; D005456:Fluorescent Dyes; D005978:Glutathione / Q000737:chemistry / Q000302:isolation & purification*; D006367:HeLa Cells; D006801:Humans; D017785:Photons; D013050:Spectrometry, Fluorescence; D013917:Thrombin / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D013917:Thrombin; D005978:Glutathione
keywords: Endogenous; Platelet; Ratiometric imaging; Two-photon fluorescent probe; Ultratrace glutathione