title: Submerged fermentation production and characterization of intracellular triterpenoids from Ganoderma lucidum using HPLC-ESI-MS.
abstract: As the main bioactive metabolites of Ganoderma lucidum, triterpenoids have various pharmacological effects. In this paper, the nutritional requirements and culture conditions of a submerged culture of G. lucidum were optimized using the response surface methodology; maximum mycelia biomass and intracellular triterpenoid production reached 1.87 g/100 ml and 93.21 mg/100 ml, respectively, for a culture consisting of wort 4.10% (0.041 g/ml) and yeast extract 1.89% (0.0189 g/ml), pH 5.40. For the first time, we established that wort, which is cheap and abundant, can replace the more commonly used glucose as the sole source of carbohydrate. Using high-performance liquid chromatography-electrospray ionization-mass spectrometry (HPLC-ESI-MS), 10 major ganoderic acids were tentatively identified based on the predominant fragmentation pathways with the elimination of H2O and CO2, as well as cleavage of the D-ring.
authors.affiliation: Zhejiang Provincial Key Laboratory of Food Microbiology, College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China.
authors.forename: Mei-lin
authors.initials: ML
authors.lastname: Cui
authors.affiliation: Zhejiang Provincial Key Laboratory of Food Microbiology, College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China.
authors.forename: Huan-yi
authors.initials: HY
authors.lastname: Yang
authors.affiliation: Zhejiang Provincial Key Laboratory of Food Microbiology, College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China.
authors.forename: Guo-qing
authors.initials: GQ
authors.lastname: He
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D015394:Molecular Structure; D009584:Nitrogen / Q000378:metabolism; D020940:Reishi / Q000254:growth & development / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D014315:Triterpenes / Q000737:chemistry / Q000378:metabolism*
chemical_list: D014315:Triterpenes; C556862:ganoderic acid; D009584:Nitrogen
keywords: Ganoderic acids; Ganoderma lucidum; HPLC-ESI-MS; Response surface methodology; Wort