title: A novel strategy for selective gene delivery by using the inhibitory effect of blue light on jetPRIME-mediated transfection.
abstract: Photodynamic control of gene delivery is a new technology with growing applications in gene therapy and basic cell research. Main approaches of light-selective gene delivery rely on the light-dependent enhancement of transfection efficiency. Studies focused on light-stimulated inhibitory regulation of transfection have rarely been reported. Here, we tried to establish a novel procedure of light-dependent inhibition of transfection. Our experiments, conducted with several types of commercial transfection reagents, revealed that jetPRIME-mediated transfection was strongly inhibited by blue light. Although the uptake of reagent-DNA complex was drastically reduced, preliminary exposure of cells or reagent-DNA complex to blue light had no inhibitory effect on the transfection efficiency. The inhibitory effect was wavelength-dependent and mediated by reactive oxygen species. Partial exposure of a culture vessel to blue light resulted in selective gene delivery into cells grown on the unexposed area of the vessel. By using this approach, different types of plasmid DNA were delivered into different areas in the culture vessel. This novel approach to the inhibitory control of transfection provides practical options for research and therapeutics. Biotechnol. Bioeng. 2016;113: 1560-1567. © 2015 Wiley Periodicals, Inc.
authors.affiliation: Department of Biochemistry, National Defense Medical College, Tokorozawa, 359-8513, Japan.
authors.forename: Minori
authors.initials: M
authors.lastname: Dateki
authors.affiliation: Department of Biochemistry, National Defense Medical College, Tokorozawa, 359-8513, Japan.
authors.forename: Osamu
authors.initials: O
authors.lastname: Imamura
authors.affiliation: Department of Biochemistry, National Defense Medical College, Tokorozawa, 359-8513, Japan.
authors.forename: Masaaki
authors.initials: M
authors.lastname: Arai
authors.affiliation: Research Faculty of Agriculture, Division of Applied Bioscience, Hokkaido University, Sapporo, Japan.
authors.forename: Hidehisa
authors.initials: H
authors.lastname: Shimizu
authors.affiliation: Department of Biochemistry, National Defense Medical College, Tokorozawa, 359-8513, Japan. ktakishi@ndmc.ac.jp.
authors.forename: Kunio
authors.initials: K
authors.lastname: Takishima
mesh_terms: D001709:Biotechnology / Q000379:methods*; D018014:Gene Transfer Techniques; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D008027:Light
keywords: blue light; jetPRIME; selective gene delivery