title: Modulation in Natriuretic Peptides System in Experimental Colitis in Rats.
abstract: BACKGROUND
  Renin-angiotensin system is involved in the pathophysiology of colonic inflammation. However, there are a few reports about modulation of natriuretic peptide system.
  
  
  OBJECTIVE
  This study investigates whether a local atrial natriuretic peptide (ANP) system exists in rat colon and whether ANP plays a role in the regulation of colonic motility in experimental colitis rat model.
  
  
  METHODS
  Experimental colitis was induced by an intake of 5 % dextran sulfate sodium (DSS) dissolved in tap water for 7 days. After rats were killed, plasma hormone concentrations and mRNAs for natriuretic peptide system were measured. Functional analysis of colonic motility in response to ANP was performed using taenia coli.
  
  
  RESULTS
  DSS-treated colon showed an increased necrosis with massive infiltration of inflammatory cells. The colonic natriuretic peptide receptor-A mRNA level and particulate guanylyl cyclase activity in response to ANP from colonic tissue membranes were higher, and the mRNA levels of ANP and natriuretic peptide receptor-B were lower in DSS-treated rats than in control rats. ANP decreased the frequency of basal motility in a dose-dependent manner but did not change the amplitude. The inhibitory responses of frequency of basal motility to ANP and 8-bromo-cGMP were enhanced in DSS-treated rat colon.
  
  
  CONCLUSIONS
  In conclusion, augmentation of inhibitory effect on basal motility by ANP in experimental colitis may be due an increased expression of colonic natriuretic peptide receptor-A mRNA. These data suggest that local natriuretic peptide system is partly involved in the pathophysiology of experimental colitis.
authors.affiliation: Department of Surgery, Research Institute for Endocrine Sciences, Chonbuk National University Medical School, Jeonju, Korea.
authors.forename: Chang Ho
authors.initials: CH
authors.lastname: Lee
authors.affiliation: Department of Surgery, Research Institute for Endocrine Sciences, Chonbuk National University Medical School, Jeonju, Korea.
authors.forename: Gi Won
authors.initials: GW
authors.lastname: Ha
authors.affiliation: Department of Surgery, Research Institute for Endocrine Sciences, Chonbuk National University Medical School, Jeonju, Korea. kimjhun@chonbuk.ac.kr.
authors.forename: Jong Hun
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Department of Physiology, Research Institute for Endocrine Sciences, Chonbuk National University Medical School, 20 Gunjiro, Dukjingu, Jeonju, 561-180, Korea. shkim@chonbuk.ac.kr.
authors.forename: Suhn Hee
authors.initials: SH
authors.lastname: Kim
mesh_terms: D000818:Animals; D009320:Atrial Natriuretic Factor / Q000097:blood*; D001835:Body Weight; D003092:Colitis / Q000139:chemically induced / Q000378:metabolism* / Q000503:physiopathology; D003106:Colon / Q000503:physiopathology*; D016264:Dextran Sulfate; D004195:Disease Models, Animal; D005769:Gastrointestinal Motility; D006162:Guanylate Cyclase / Q000378:metabolism; D008297:Male; D017207:Rats, Sprague-Dawley; D017461:Receptors, Atrial Natriuretic Factor / Q000378:metabolism; D012083:Renin / Q000097:blood*; D012084:Renin-Angiotensin System
chemical_list: D009320:Atrial Natriuretic Factor; D016264:Dextran Sulfate; D012083:Renin; D006162:Guanylate Cyclase; D017461:Receptors, Atrial Natriuretic Factor
keywords: ANP; Colitis; Motility; Natriuretic peptide receptor; cGMP