title: Cystofilobasidium intermediumsp. nov. andCystofilobasidium alribaticumf.a. sp. nov., isolated from Mediterranean forest soils.
abstract: Multiple isolates belonging to the basidiomycetous genusCystofilobasidiumwere obtained from forest soils in Serra da Arrábida Natural Park in Portugal. Phylogenetic analyses employing concatenated sequences of the D1/D2 domain and ITS region support the recognition of two novel species:Cystofilobasidium alribaticumf.a., sp. nov. (type strain CBS 14164T= PYCC 6956T= DSM 101473T) andCystofilobasidium intermediumsp. nov. (type strain CBS 14089T= PYCC 6856T= DSM 101474T). WhereasC. alribaticumf. a. sp. nov. does not form hyphae, even when different strains are crossed,C. intermediumsp. nov. is self-fertile and forms mycelium with teliospores that upon germination give rise to slender basidia. The most remarkable physiological trait of the two novel species is their ability to grow at 35 °C, a property not observed for remaining species of the genus.
authors.affiliation: UCIBIO-REQUIMTE, Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Caparica, Portugal.
authors.forename: Ana
authors.initials: A
authors.lastname: Pontes
authors.affiliation: UCIBIO-REQUIMTE, Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Caparica, Portugal.|Geobotany, Faculty for Biology and Biotechnology, Ruhr-Universität Bochum, Bochum, Germany.
authors.forename: Oliver
authors.initials: O
authors.lastname: Röhl
authors.affiliation: UCIBIO-REQUIMTE, Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Caparica, Portugal.
authors.forename: Cláudia
authors.initials: C
authors.lastname: Carvalho
authors.affiliation: UCIBIO-REQUIMTE, Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Caparica, Portugal.
authors.forename: Cristina
authors.initials: C
authors.lastname: Maldonado
authors.affiliation: Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Brunswick, Germany.
authors.forename: Andrey M
authors.initials: AM
authors.lastname: Yurkov
authors.affiliation: UCIBIO-REQUIMTE, Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Caparica, Portugal.
authors.forename: José
authors.initials: J
authors.lastname: Paulo Sampaio