title: Metabolomic profile related to cardiovascular disease in patients with type 2 diabetes mellitus: A pilot study.
abstract: Type 2 diabetes mellitus (T2DM) patients have an increased risk of cardiovascular disease (CVD) that represents one of the main causes of mortality in this population. The knowledge of the underlie factors involved in the development of CVD and the discovery of new biomarkers of the disease could help to early identification of high-risk patients. Using liquid chromatography coupled to high resolution mass spectrometry (LC-HRMS) we analyzed the serum metabolomic profile of 30 subject distributed according three groups: (i) T2DM patients with CVD; (ii) T2DM patients without CVD; (iii) non-diabetic subjects as controls (C) in order to identify potential biomarkers of the CVD related to T2DM. A partial least squares discriminant analysis (PLS-DA) and one-way analysis of variance (ANOVA) were applied to identify differential metabolites between different groups. Four glycerophospholipids were further identiﬁed as potential biomarkers of CVD in T2DM patients. Specifically, a reduction in phosphatidylcholine, lysophosphatidylcholine and lysophosphatidylethanolamine (LPE) serum levels were found in T2DM patients compared to controls, presenting the patients with CVD the lowest serum levels of these metabolites. These results show a generalized reduction of circulating phospholipids species in T2DM patients which is more pronounced in those with CVD providing information of the pathways involved in the pathogenesis and progression of CVD associated to T2DM.
authors.affiliation: Bone Metabolic Unit (RETICEF), Endocrinology Unit, Instituto de Investigación Biosanitaria (Ibs) de Granada, Hospital Universitario San Cecilio, Avda. Doctor Oloriz 16, 18012 Granada, Spain. Electronic address: bgfontana@fibao.es.
authors.forename: Beatriz
authors.initials: B
authors.lastname: García-Fontana
authors.affiliation: Bone Metabolic Unit (RETICEF), Endocrinology Unit, Instituto de Investigación Biosanitaria (Ibs) de Granada, Hospital Universitario San Cecilio, Avda. Doctor Oloriz 16, 18012 Granada, Spain; Proteomic Research Service, Instituto de Investigación Biosanitaria (Ibs) de Granada, Hospital Universitario San Cecilio, Avda. Doctor Oloriz 16, 18012, Granada, Spain. Electronic address: sonia.morales.exts@juntadeandalucia.es.
authors.forename: Sonia
authors.initials: S
authors.lastname: Morales-Santana
authors.affiliation: Fundación MEDINA, Centro de Excelencia en Investigación de Medicamentos Innovadores en Andalucía. Parque Tecnológico de Ciencias de la Salud, Av del Conocimiento, 34, 18016 Armilla, Granada, Spain. Electronic address: caridad.diaz@medinaandalucia.es.
authors.forename: Caridad
authors.initials: C
authors.lastname: Díaz Navarro
authors.affiliation: Endocrinology Division, Hospital General de Ciudad Real, Calle del Obispo Rafael Torija, s/n, 13005 Ciudad Real, Spain. Electronic address: pedrorozasm@yahoo.es.
authors.forename: Pedro
authors.initials: P
authors.lastname: Rozas-Moreno
authors.affiliation: Fundación MEDINA, Centro de Excelencia en Investigación de Medicamentos Innovadores en Andalucía. Parque Tecnológico de Ciencias de la Salud, Av del Conocimiento, 34, 18016 Armilla, Granada, Spain. Electronic address: olga.genilloud@medinaandalucia.es.
authors.forename: Olga
authors.initials: O
authors.lastname: Genilloud
authors.affiliation: Fundación MEDINA, Centro de Excelencia en Investigación de Medicamentos Innovadores en Andalucía. Parque Tecnológico de Ciencias de la Salud, Av del Conocimiento, 34, 18016 Armilla, Granada, Spain.
authors.forename: Francisca
authors.initials: F
authors.lastname: Vicente Pérez
authors.affiliation: Fundación MEDINA, Centro de Excelencia en Investigación de Medicamentos Innovadores en Andalucía. Parque Tecnológico de Ciencias de la Salud, Av del Conocimiento, 34, 18016 Armilla, Granada, Spain. Electronic address: jose.perezdelpalacio@medinaandalucia.es.
authors.forename: José
authors.initials: J
authors.lastname: Pérez del Palacio
authors.affiliation: Bone Metabolic Unit (RETICEF), Endocrinology Unit, Instituto de Investigación Biosanitaria (Ibs) de Granada, Hospital Universitario San Cecilio, Avda. Doctor Oloriz 16, 18012 Granada, Spain. Electronic address: mmt@mamuto.es.
authors.forename: Mnuel
authors.initials: M
authors.lastname: Muñoz-Torres
mesh_terms: D002318:Cardiovascular Diseases / Q000097:blood* / Q000175:diagnosis* / Q000453:epidemiology; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003430:Cross-Sectional Studies; D003924:Diabetes Mellitus, Type 2 / Q000097:blood* / Q000175:diagnosis* / Q000453:epidemiology; D006801:Humans; D008297:Male; D055432:Metabolomics / Q000379:methods*; D008875:Middle Aged; D010865:Pilot Projects; D053719:Tandem Mass Spectrometry / Q000379:methods*
keywords: Cardiovascular disease; Metabolomic; Phospholipids; Type 2 diabetes mellitus