title: Rutin ameliorates methotrexate induced hepatic injury in rats.
abstract: OBJECTIVE
  To investigate the possible protective effect of rutin on methotrexate induced hepatotoxicity in rats.
  
  
  METHODS
  Twenty-two rats were divided into three experimental groups; Control-saline, Mtx, Mtx+Rutin. Hepatic tissue was taken for histological assessment and biochemical assays. Oxidative stress parameters malondialdehyde (MDA), glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) were investigated. Liver markers aspartate aminotransferase (AST), alanine aminotransferase (ALT) were analyzed in serum.
  
  
  RESULTS
  Mtx+Rutin group showed lower histological injury compared to Mtx group, MDA and ALT levels were increased, while SOD and GSH-Px were decreased in Mtx group compared with Control-saline group. MDA and ALT levels were increased, while SOD and GSH-Px were decreased in Mtx group, compared with Mtx +Rutin group. Serum AST levels were similar among the groups.
  
  
  CONCLUSIONS
  Rutin may be a potential adjuvant drug to reduce the hepatic side effects observed during Mtx therapy for various clinical conditions.
authors.affiliation: Gulhane Military Medical Academy, Ankara, Turkey.
authors.forename: Esra
authors.initials: E
authors.lastname: Erdogan
authors.affiliation: Gulhane Military Medical Academy, Ankara, Turkey.
authors.forename: Yasin
authors.initials: Y
authors.lastname: Ilgaz
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, Ordu University, Ordu, Turkey.
authors.forename: Pinar Naile
authors.initials: PN
authors.lastname: Gurgor
authors.affiliation: Department of Medical Biochemistry, Faculty of Medicine, Hacettepe University, Ankara, Turkey.
authors.forename: Yesim
authors.initials: Y
authors.lastname: Oztas
authors.affiliation: Department of Physiology, Hacettepe University, Ankara, Turkey.
authors.forename: Turgut
authors.initials: T
authors.lastname: Topal
authors.affiliation: Gulhane Military Medical Academy, Ankara, Turkey.
authors.forename: Emin
authors.initials: E
authors.lastname: Oztas
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use; D001219:Aspartate Aminotransferases / Q000097:blood; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy* / Q000473:pathology / Q000517:prevention & control; D005260:Female; D005979:Glutathione Peroxidase / Q000032:analysis; D007166:Immunosuppressive Agents / Q000633:toxicity*; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008315:Malondialdehyde / Q000032:analysis; D008727:Methotrexate / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012431:Rutin / Q000494:pharmacology / Q000627:therapeutic use*; D013482:Superoxide Dismutase / Q000032:analysis
chemical_list: D000975:Antioxidants; D007166:Immunosuppressive Agents; D008315:Malondialdehyde; D012431:Rutin; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D008727:Methotrexate