title: The differential expression of mRNAs and long noncoding RNAs between ectopic and eutopic endometria provides new insights into adenomyosis.
abstract: Adenomyosis, defined as ectopic endometrial tissue within the myometrium, can often be misdiagnosed as multiple uterine leiomyomata or endometrial thickening. We therefore performed a combined mRNA and long noncoding (lnc)RNA microarray and bioinformatic analysis of eutopic and ectopic endometria in women with adenomyosis to better understand its pathogenesis and help in the development of a semi-invasive diagnostic test. A total of 586 mRNAs were increased and 305 mRNAs decreased in the ectopic endometrium of adenomyosis compared with the eutopic endometrium, while 388 lncRNA transcripts were up-regulated and 188 down-regulated in ectopic compared with paired eutopic endometrial tissue. Bioinformatic analysis suggested a series of metabolic and molecular abnormalities in adenomyosis, which have many similarities with endometriosis. Furthermore, our study constitutes the first known report of lncRNA expression patterns in human adenomyosis ectopic and eutopic endometrial tissue.
authors.affiliation: State Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, Nanjing, China. huoran@njmu.edu.cn jesse_1982@163.com.
authors.forename: Cheng
authors.initials: C
authors.lastname: Zhou
authors.affiliation: Wuxi Maternity and Child Health Hospital, Wuxi city, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, Nanjing, China. huoran@njmu.edu.cn jesse_1982@163.com.
authors.forename: Fei
authors.initials: F
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, Nanjing, China. huoran@njmu.edu.cn jesse_1982@163.com.
authors.forename: Ji
authors.initials: J
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, Nanjing, China. huoran@njmu.edu.cn jesse_1982@163.com.
authors.forename: Xiaobei
authors.initials: X
authors.lastname: Ni
authors.affiliation: State Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, Nanjing, China. huoran@njmu.edu.cn jesse_1982@163.com.
authors.forename: Ran
authors.initials: R
authors.lastname: Huo
authors.affiliation: State Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, Nanjing, China. huoran@njmu.edu.cn jesse_1982@163.com and State Key Laboratory of Reproductive Medicine, Nanjing Maternity and Child Health Hospital, Nanjing Medical University, Nanjing, China.
authors.forename: Zhonghua
authors.initials: Z
authors.lastname: Shi
mesh_terms: D062788:Adenomyosis / Q000175:diagnosis / Q000378:metabolism*; D000328:Adult; D015415:Biomarkers / Q000378:metabolism; D004717:Endometrium / Q000378:metabolism* / Q000473:pathology; D005260:Female; D015870:Gene Expression; D063990:Gene Ontology; D006801:Humans; D008875:Middle Aged; D062085:RNA, Long Noncoding / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D046888:Tissue Array Analysis
chemical_list: D015415:Biomarkers; D062085:RNA, Long Noncoding; D012333:RNA, Messenger