title: Synthesis of a far-red fluorophore and its use as an esterase probe in living cells.
abstract: We report the synthesis of a new far-red fluorophore, 1,3-dichloro-7-hydroxy-2H-spiro[acridine-9,1'-cyclohexane]-2',5'-diene-2,4'-dione (DSACO), which was modified to make two esterase probes: DSACO-2-AME and DSACO-7-AME. Both probes act as "turn-on" substrates for esterases and lipases. DSACO-2-AME exhibited efficient esterase-activated fluorescence inside living cells and is a stable, far-red alternative for the widely-used fluorescein diacetate.
authors.affiliation: Department of Biomedical Engineering, OHSU Center for Spatial Systems Biomedicine, Oregon Health & Science University, 2730 SW Moody Avenue, CL3B, Portland, OR 97201, USA. beattyk@ohsu.edu.
authors.forename: Samantha R
authors.initials: SR
authors.lastname: Levine
authors.affiliation: Department of Biomedical Engineering, OHSU Center for Spatial Systems Biomedicine, Oregon Health & Science University, 2730 SW Moody Avenue, CL3B, Portland, OR 97201, USA. beattyk@ohsu.edu.
authors.forename: Kimberly E
authors.initials: KE
authors.lastname: Beatty
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004950:Esterases / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D051381:Rats
chemical_list: D005456:Fluorescent Dyes; D004950:Esterases