title: Bacillus cucumissp. nov. isolated from the rhizosphere of cucumber (Cucumis sativus).
abstract: A facultative anaerobic, Gram-positive staining, endospore-forming bacterium, isolated from the rhizosphere of cucumber (Cucumis sativus), was taxonomically investigated. Based on 16S rRNA gene sequence similarity comparisons, strain AP-6Tclustered together with other species of the genusBacillusand showed highest similarities withBacillus drentensisLMG 21831T(99.1 %),Bacillus viretiLMG 21834T(98.7 %) andBacillus soliLMG 21838T(98.5 %). The 16S rRNA gene sequence similarity to the sequences of the type strains of other species of the genusBacilluswas 98.5 % or less. Chemotaxonomic features supported the grouping of the strain in the genusBacillus; for example, the major fatty acids were anteiso-C15 : 0, iso-C15 : 0and C16 : 0, the polar lipid profile contained the major components diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unidentified glycolipid, the major quinone was menaquinone MK-7 and the major compound in the polyamine pattern was spermidine. Additionally, DNA-DNA hybridization withB. drentensisLMG 21831T,B. viretiLMG 21834TandB. soliLMG 21838Tresulted in relatedness values that were clearly below 70 %. Physiological and biochemical test results were also different from those of the most closely related species. As a consequence, AP-6Trepresents a novel species of the genusBacillus, for which the nameBacillus cucumissp. nov. is proposed, with AP-6T( = CIP 110974T= CCM 8651T) as the type strain.
authors.affiliation: Institut für Angewandte Mikrobiologie, Universität Giessen, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Kämpfer
authors.affiliation: Institut für Mikrobiologie, Veterinärmedizinische Universität, A-1210 Wien, Austria.
authors.forename: Hans-Jürgen
authors.initials: HJ
authors.lastname: Busse
authors.affiliation: Institut für Angewandte Mikrobiologie, Universität Giessen, Germany.
authors.forename: Stefanie P
authors.initials: SP
authors.lastname: Glaeser
authors.affiliation: Department of Entomology and Plant Pathology, Auburn University, AL 36849, USA.
authors.forename: Joseph W
authors.initials: JW
authors.lastname: Kloepper
authors.affiliation: Department of Entomology and Plant Pathology, Auburn University, AL 36849, USA.
authors.forename: Chia-Hui
authors.initials: CH
authors.lastname: Hu
authors.affiliation: Department of Entomology and Plant Pathology, Auburn University, AL 36849, USA.
authors.forename: John A
authors.initials: JA
authors.lastname: McInroy