title: Gene Expression Analysis in the Age of Mass Sequencing: An Introduction.
abstract: During the last years the technology used for gene expression analysis has changed dramatically. The old mainstay, DNA microarray, has served its due course and will soon be replaced by next-generation sequencing (NGS), the Swiss army knife of modern high-throughput nucleic acid-based analysis. Therefore preparation technologies have to adapt to suit the emerging NGS technology platform. Moreover, interpretation of the results is still time consuming and employs the use of high-end computers usually not found in molecular biology laboratories. Alternatively, cloud computing might solve this problem. Nevertheless, these new challenges have to be embraced for gene expression analysis in general.
authors.affiliation: Department of Surgery, TU Dresden, Fetscherstr. 74, Dresden, 01307, Germany. christian.pilarsky@gmail.com.
authors.forename: Christian
authors.initials: C
authors.lastname: Pilarsky
authors.affiliation: Department of Surgery, TU Dresden, Fetscherstr. 74, Dresden, 01307, Germany.
authors.forename: Lahiri Kanth
authors.initials: LK
authors.lastname: Nanduri
authors.affiliation: Biotechnology Center, Technische Universität Dresden, Tatzberg 47-49, Dresden, 01307, Germany.
authors.forename: Janine
authors.initials: J
authors.lastname: Roy
mesh_terms: D020869:Gene Expression Profiling / Q000379:methods; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006801:Humans; D012313:RNA / Q000235:genetics; D017423:Sequence Analysis, RNA / Q000379:methods*; D012984:Software; D057188:Workflow
chemical_list: D012313:RNA
keywords: Next-generation sequencing; RNA -seq