title: Surfactant Proteins SP-A and SP-D Modulate Uterine Contractile Events in ULTR Myometrial Cell Line.
abstract: Pulmonary surfactant proteins SP-A and SP-D are pattern recognition innate immune molecules. However, there is extrapulmonary existence, especially in the amniotic fluid and at the feto-maternal interface. There is sufficient evidence to suggest that SP-A and SP-D are involved in the initiation of labour. This is of great importance given that preterm birth is associated with increased mortality and morbidity. In this study, we investigated the effects of recombinant forms of SP-A and SP-D (rhSP-A and rhSP-D, the comprising of trimeric lectin domain) on contractile events in vitro, using a human myometrial cell line (ULTR) as an experimental model. Treatment with rhSP-A or rhSP-D increased the cell velocity, distance travelled and displacement by ULTR cells. rhSP-A and rhSP-D also affected the contractile response of ULTRs when grown on collagen matrices showing reduced surface area. We investigated this effect further by measuring contractility-associated protein (CAP) genes. Treatment with rhSP-A and rhSP-D induced expression of oxytocin receptor (OXTR) and connexin 43 (CX43). In addition, rhSP-A and rhSP-D were able to induce secretion of GROα and IL-8. rhSP-D also induced the expression of IL-6 and IL-6 Ra. We provide evidence that SP-A and SP-D play a key role in modulating events prior to labour by reconditioning the human myometrium and in inducing CAP genes and pro-inflammatory cytokines thus shifting the uterus from a quiescent state to a contractile one.
authors.affiliation: Centre for Infection, Immunity and Disease Mechanisms, College of Health and Life Sciences, Brunel University London, Uxbridge, UB8 3PH, United Kingdom.
authors.forename: Georgios
authors.initials: G
authors.lastname: Sotiriadis
authors.affiliation: Centre for Infection, Immunity and Disease Mechanisms, College of Health and Life Sciences, Brunel University London, Uxbridge, UB8 3PH, United Kingdom.
authors.forename: Eswari
authors.initials: E
authors.lastname: Dodagatta-Marri
authors.affiliation: Centre for Infection, Immunity and Disease Mechanisms, College of Health and Life Sciences, Brunel University London, Uxbridge, UB8 3PH, United Kingdom.
authors.forename: Lubna
authors.initials: L
authors.lastname: Kouser
authors.affiliation: Department of Infection and Immunity, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia.
authors.forename: Fatimah S
authors.initials: FS
authors.lastname: Alhamlan
authors.affiliation: Centre for Infection, Immunity and Disease Mechanisms, College of Health and Life Sciences, Brunel University London, Uxbridge, UB8 3PH, United Kingdom.
authors.forename: Uday
authors.initials: U
authors.lastname: Kishore
authors.affiliation: Centre for Infection, Immunity and Disease Mechanisms, College of Health and Life Sciences, Brunel University London, Uxbridge, UB8 3PH, United Kingdom.|Institute of Environment, Heath and Societies, Brunel University London, Uxbridge, UB8 3PH, United Kingdom.
authors.forename: Emmanouil
authors.initials: E
authors.lastname: Karteris
mesh_terms: D000653:Amniotic Fluid / Q000378:metabolism; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D018031:Connexin 43 / Q000235:genetics; D016207:Cytokines / Q000378:metabolism; D005260:Female; D006801:Humans; D009215:Myometrium / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D036801:Parturition; D037662:Pulmonary Surfactant-Associated Protein A / Q000378:metabolism / Q000494:pharmacology*; D037663:Pulmonary Surfactant-Associated Protein D / Q000378:metabolism / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D018045:Receptors, Oxytocin / Q000235:genetics; D011994:Recombinant Proteins / Q000494:pharmacology; D014590:Uterine Contraction / Q000187:drug effects* / Q000378:metabolism; D014945:Wound Healing / Q000187:drug effects
chemical_list: D018031:Connexin 43; D016207:Cytokines; C500216:GJA1 protein, human; C569600:OXTR protein, human; D037662:Pulmonary Surfactant-Associated Protein A; D037663:Pulmonary Surfactant-Associated Protein D; D012333:RNA, Messenger; D018045:Receptors, Oxytocin; D011994:Recombinant Proteins