title: [Recombinant influenza A virus NS2 protein inhibits the production of interferon in lung tissues of infected mice].
abstract: OBJECTIVE
  To obtain non-structural protein 2 (NS2) of influenza A virus (IAV) via the expression system of Escherichia coli, and observe the effect of NS2 on the production of interferon γ (IFN-γ) in mouse lung tissues.
  
  
  METHODS
  A prokaryotic expression plasmid pET22b(+)/NS2 was constructed and transformed into Rosetta-gami(2) of Escherichia coli. Through the inducible expression and purification, NS2 was prepared. Forty BALB/c mice were divided randomly into 8 groups, normal control group, 200 μg/kg NS2 joint virus group, 200 μg/kg NS2 joint RNA group, 100 μg/kg inactivated virus group, 0.2×lethal dose 50% (LD50) virus group, viral RNA group of 50 μg/kg, 200 μg/kg NS2 group, and 100 μg/kg NS2 group. All mice were subjected to nasal inhalation. Reverse transcription-PCR and Western blotting were respectively performed to detect the mRNA and protein expressions of IFN-γ in lung tissues.
  
  
  RESULTS
  Recombinant NS2 was obtained by the induction of isopropyl-beta-D-thiogalactopyranoside (IPTG) and purification. After the nasal inhalation of NS2 or/and IAV to mice, reverse transcription-PCR and Western blotting showed that the levels of IFN-γ mRNA and protein in the lung tissues of NS2 joint virus group were lower than those of the virus group.
  
  
  CONCLUSIONS
  NS2 could inhibit the expressions of IFN-γ mRNA and protein in lung tissues of mice infected by IAV.
authors.affiliation: Department of Microbiology, Guizhou Medical University, Guiyang 550004, China.
authors.forename: Yanxin
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Department of Microbiology, Guizhou Medical University, Guiyang 550004, China.
authors.forename: Qiuju
authors.initials: Q
authors.lastname: Mou
authors.affiliation: Department of Microbiology, Guizhou Medical University, Guiyang 550004, China.
authors.forename: Bingbing
authors.initials: B
authors.lastname: Zhao
authors.affiliation: Experimental Animal Center, Guizhou Medical University, Guiyang 550004, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Luo
authors.affiliation: Experimental Animal Center, Guizhou Medical University, Guiyang 550004, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhi
authors.affiliation: Experimental Animal Center, Guizhou Medical University, Guiyang 550004, China.
authors.forename: Lusha
authors.initials: L
authors.lastname: Lan
authors.affiliation: [1]Department of Microbiology, [2]Experimental Animal Center, Guizhou Medical University, Guiyang 550004, China. *Corresponding author, E-mail: 624057523@qq.com.
authors.forename: Yan
authors.initials: Y
authors.lastname: Jiang
mesh_terms: D000818:Animals; D015536:Down-Regulation; D005260:Female; D054884:Host-Pathogen Interactions; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics / Q000378:metabolism*; D007251:Influenza, Human / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D007371:Interferon-gamma / Q000235:genetics / Q000378:metabolism*; D008168:Lung / Q000378:metabolism* / Q000821:virology; D051379:Mice; D008807:Mice, Inbred BALB C; D017361:Viral Nonstructural Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: C556232:NS2 protein, influenza virus A; D017361:Viral Nonstructural Proteins; D007371:Interferon-gamma