title: Islet Autoantibody Detection by Electrochemiluminescence (ECL) Assay.
abstract: Two fundamental aspects for precisely predicting the risk of developing type 1 diabetes by islet autoantibodies are assay sensitivity and disease specificity. We have recently developed electrochemiluminescent (ECL) insulin autoantibody (IAA) and GAD65 autoantibody (GADA) assays. ECL assays are sensitive, able to identify the initiation of islet autoimmunity earlier in life among high-risk young children before clinical onset of diabetes and are more disease specific because they are able to discriminate high-affinity, high-risk diabetes specific islet autoantibodies from low-affinity, low-risk autoantibodies.
authors.affiliation: Barbara Davis Center for Childhood Diabetes, University of Colorado School of Medicine, Building 500-13001 E. 17th Place, Campus Box C290, Room E1354, Aurora, CO, 80045, USA. Liping.yu@ucdenver.edu.
authors.forename: Liping
authors.initials: L
authors.lastname: Yu
mesh_terms: D001323:Autoantibodies / Q000032:analysis*; D003922:Diabetes Mellitus, Type 1 / Q000175:diagnosis* / Q000276:immunology; D055664:Electrochemical Techniques / Q000379:methods*; D005968:Glutamate Decarboxylase / Q000276:immunology; D006801:Humans; D007515:Islets of Langerhans / Q000276:immunology*; D008163:Luminescent Measurements / Q000379:methods*
chemical_list: D001323:Autoantibodies; D005968:Glutamate Decarboxylase
keywords: Assay; Autoantibodies; Diabetes; Prediction