title: Progesterone reduces brain mitochondrial dysfunction after transient focal ischemia in male and female mice.
abstract: This study investigated the effect of intranasal administration of progesterone on the early brain mitochondrial respiratory chain dysfunction and oxidative damage after transient middle cerebral occlusion in male and female mice. We showed that progesterone (8 mg/kg at 1 h post-middle cerebral occlusion) restored the mitochondrial reduced glutathione pool and the nicotinamide adenine dinucleotide-linked respiration in both sexes. Progesterone also reversed the decrease of the flavin adenine dinucleotide-linked respiration, which was only observed in females. Our findings point to a sex difference in stroke effects on the brain respiratory chain and suggest that the actions of progesterone on mitochondrial function may participate in its neuroprotective properties.
authors.affiliation: U1195 Inserm and University Paris-Sud, Le Kremlin-Bicêtre, France Biochemistry Laboratory, Bicêtre Hospital, Assistance-Publique Hôpitaux de Paris, University Paris-Sud, Le Kremlin-Bicêtre Cedex, France.
authors.forename: Pauline
authors.initials: P
authors.lastname: Gaignard
authors.affiliation: U1195 Inserm and University Paris-Sud, Le Kremlin-Bicêtre, France.
authors.forename: Magalie
authors.initials: M
authors.lastname: Fréchou
authors.affiliation: U1195 Inserm and University Paris-Sud, Le Kremlin-Bicêtre, France.
authors.forename: Michael
authors.initials: M
authors.lastname: Schumacher
authors.affiliation: Biochemistry Laboratory, Bicêtre Hospital, Assistance-Publique Hôpitaux de Paris, University Paris-Sud, Le Kremlin-Bicêtre Cedex, France.
authors.forename: Patrice
authors.initials: P
authors.lastname: Thérond
authors.affiliation: M et P Pharma AG, Emmetten, Switzerland.
authors.forename: Claudia
authors.initials: C
authors.lastname: Mattern
authors.affiliation: Biochemistry Laboratory, Bicêtre Hospital, Assistance-Publique Hôpitaux de Paris, University Paris-Sud, Le Kremlin-Bicêtre Cedex, France.
authors.forename: Abdelhamid
authors.initials: A
authors.lastname: Slama
authors.affiliation: U1195 Inserm and University Paris-Sud, Le Kremlin-Bicêtre, France rachida.guennoun@inserm.fr.
authors.forename: Rachida
authors.initials: R
authors.lastname: Guennoun
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D019069:Cell Respiration / Q000187:drug effects; D005260:Female; D005182:Flavin-Adenine Dinucleotide / Q000031:analogs & derivatives / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D020244:Infarction, Middle Cerebral Artery / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D009243:NAD / Q000378:metabolism; D018696:Neuroprotective Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D018384:Oxidative Stress / Q000187:drug effects*; D010101:Oxygen Consumption / Q000187:drug effects; D011374:Progesterone / Q000008:administration & dosage / Q000627:therapeutic use*
chemical_list: D018696:Neuroprotective Agents; D009243:NAD; D005182:Flavin-Adenine Dinucleotide; C058805:1,5-dihydro-FAD; D011374:Progesterone; D005978:Glutathione
keywords: Mitochondria; oxidative phosphorylation; oxidative stress; progesterone; sex difference