title: α-Synuclein aggregation in the olfactory bulb of middle-aged common marmoset.
abstract: The synaptic protein α-synuclein has been identified as a major component of Lewy bodies, a pathological hallmark of Parkinson's disease (PD). Prior to the formation of Lewy bodies, mislocalization and aggregation of the α-synuclein in brain tissue is frequently observed in various neurodegenerative diseases. Aberrant accumulation and localization of α-synuclein are also observed in the aging human brain, for which reason aging is regarded as a risk factor for neurodegenerative disease. To investigate changes in α-synuclein properties in the aging brain, we compared α-synuclein immunoreactivity in brain tissue of young (2-years-old) and middle-aged (6-years-old) common marmoset (Callithrix jacchus). Our analyses revealed marked changes in α-synuclein immunoreactivity in the olfactory bulb of common marmosets of these age cohorts. Perikaryal α-synuclein aggregations were formed in the olfactory bulb in middle-aged animals. We also observed signals of α-synuclein accumulation in hippocampus in this cohort; however, unlike in the olfactory bulb, hippocampal α-synuclein signals were localized in the synaptic terminals. We did not observe either of these features in younger marmosets, which suggest that aging may play a role in these phenomena. Our results using common marmoset brain corresponded with the observation that the α-synuclein aggregations were first occurred from olfactory bulb in human normal aged and PD brain. Therefore, common marmoset is expected as useful model for α-synuclein pathology.
authors.affiliation: Department of Physiology, Keio University School of Medicine, Shinjuku, Tokyo, Japan.
authors.forename: Reona
authors.initials: R
authors.lastname: Kobayashi
authors.affiliation: Department of Neuropathology, Brain Bank for Aging Research, Tokyo Metropolitan Institute of Gerontology, Itabashi-ku, Tokyo, Japan.
authors.forename: Junko
authors.initials: J
authors.lastname: Takahashi-Fujigasaki
authors.affiliation: Department of Physiology, Keio University School of Medicine, Shinjuku, Tokyo, Japan; RIKEN Brain Science Institute Laboratory for Marmoset Neural Architecture, Wako-shi, Saitama, Japan.
authors.forename: Seiji
authors.initials: S
authors.lastname: Shiozawa
authors.affiliation: Department of Physiology, Keio University School of Medicine, Shinjuku, Tokyo, Japan; Division of Regenerative Medicine, Jikei University School of Medicine, Minato-ku, Tokyo, Japan.
authors.forename: Chikako
authors.initials: C
authors.lastname: Hara-Miyauchi
authors.affiliation: Central Institute for Experimental Animals, Kawasaki, Kanagawa, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Inoue
authors.affiliation: Department of Physiology, Keio University School of Medicine, Shinjuku, Tokyo, Japan; Division of Regenerative Medicine, Jikei University School of Medicine, Minato-ku, Tokyo, Japan.
authors.forename: Hirotaka James
authors.initials: HJ
authors.lastname: Okano
authors.affiliation: Department of Physiology, Keio University School of Medicine, Shinjuku, Tokyo, Japan; RIKEN Brain Science Institute Laboratory for Marmoset Neural Architecture, Wako-shi, Saitama, Japan; Central Institute for Experimental Animals, Kawasaki, Kanagawa, Japan.
authors.forename: Erika
authors.initials: E
authors.lastname: Sasaki
authors.affiliation: Department of Physiology, Keio University School of Medicine, Shinjuku, Tokyo, Japan. Electronic address: hidokano@a2.keio.jp.
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Okano
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism; D002144:Callithrix; D005260:Female; D009830:Olfactory Bulb / Q000378:metabolism*; D066329:Protein Aggregates; D013347:Subcellular Fractions / Q000378:metabolism; D051844:alpha-Synuclein / Q000378:metabolism*
chemical_list: D066329:Protein Aggregates; D051844:alpha-Synuclein
keywords: Common marmoset; Olfactory bulb; Parkinson's disease; α-Synuclein