title: Altered expression profile of apoptosis-related molecules correlated with clinicopathological factors in non-small-cell lung cancer.
abstract: Apoptosis-related molecules can be abnormally expressed in cancers and underscore the hallmark of resisting cell death in cancer cells. This study was aimed to observe the expression patterns of apoptosis-related molecules in lung cancer and paired non-cancerous tissues, and to observe if there is a correlation between the expression of these apoptotic molecules and clinicopathologic parameters. Immunohistochemistry (IHC) was performed to analyze the expression level of CASP3, CASP8, CASP9, PARP1, Cleaved CASP3 (C-CASP3), Cleaved PARP1 (C-PARP1), XIAP, BIRC5 (Survivin) and BCL2 in lung cancer and paired non-cancerous tissues. We found that apoptosis-related molecules CASP3, CASP9, BCL2, BIRC5 and PARP1 are abnormally expressed in lung cancer cells and their expression were correlated with histology. BCL2, BIRC5 and PARP1 are expressed at higher levels in SCC than in non-SCC. C-PARP1 expression might be an independent prognostic factor for NSCLC.
authors.affiliation: Cancer Center of Guangzhou Medical University Guangzhou 510095, China.
authors.forename: Jian-Qing
authors.initials: JQ
authors.lastname: Huang
authors.affiliation: Cancer Center of Guangzhou Medical University Guangzhou 510095, China.
authors.forename: Hong-Ling
authors.initials: HL
authors.lastname: Liang
authors.affiliation: Cancer Center of Guangzhou Medical University Guangzhou 510095, China.
authors.forename: Tian-En
authors.initials: TE
authors.lastname: Jin
authors.affiliation: Guangdong Lung Cancer Institute, Guangdong General Hospital Guangzhou 510080, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Xie
mesh_terms: D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins / Q000032:analysis*; D014408:Biomarkers, Tumor / Q000032:analysis*; D002289:Carcinoma, Non-Small-Cell Lung / Q000737:chemistry* / Q000401:mortality / Q000473:pathology / Q000628:therapy; D020169:Caspases / Q000032:analysis; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D051034:Inhibitor of Apoptosis Proteins / Q000032:analysis; D053208:Kaplan-Meier Estimate; D008175:Lung Neoplasms / Q000737:chemistry* / Q000401:mortality / Q000473:pathology / Q000628:therapy; D008297:Male; D008875:Middle Aged; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases / Q000032:analysis; D011379:Prognosis; D016016:Proportional Hazards Models; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis; D012307:Risk Factors; D000077022:Survivin; D046888:Tissue Array Analysis; D051636:X-Linked Inhibitor of Apoptosis Protein / Q000032:analysis
chemical_list: D051017:Apoptosis Regulatory Proteins; C000595707:BCL2 protein, human; C107489:BIRC5 protein, human; D014408:Biomarkers, Tumor; D051034:Inhibitor of Apoptosis Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D000077022:Survivin; D051636:X-Linked Inhibitor of Apoptosis Protein; C496866:XIAP protein, human; C499320:PARP1 protein, human; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases; D020169:Caspases
keywords: Apoptosis; BCL2; PARP1; caspase 3; lung cancer