title: Whole-body vibration improves fracture healing and bone quality in rats with ovariectomy-induced osteoporosis.
abstract: OBJECTIVE
  To investigate the effect of vibration therapy on the bone callus of fractured femurs and the bone quality of intact femurs in ovariectomized rats.
  
  
  METHODS
  Fifty-six rats aged seven weeks were divided into four groups: control with femoral fracture (CON, n=14), ovariectomized with femoral fracture (OVX, n=14), control with femoral fracture plus vibration therapy (CON+VT, n=14), and ovariectomized with femoral fracture plus vibration therapy (OVX+VT, n=14). Three months after ovariectomy or sham surgery, a complete fracture was produced at the femoral mid-diaphysis and stabilized with a 1-mm-diameter intramedullary Kirschner wire. X-rays confirmed the fracture alignment and fixation. Three days later, the VT groups underwent vibration therapy (1 mm, 60 Hz for 20 minutes, three times per week for 14 or 28 days). The bone and callus quality were assessed by densitometry, three-dimensional microstructure, and mechanical test.
  
  
  RESULTS
  Ovariectomized rats exhibited a substantial loss of bone mass and severe impairment in bone microarchitecture, both in the non-fractured femur and the bone callus. Whole-body vibration therapy exerted an important role in ameliorating the bone and fracture callus parameters in the osteoporotic bone.
  
  
  CONCLUSIONS
  Vibration therapy improved bone quality and the quality of the fracture bone callus in ovariectomized rats.
authors.affiliation: Faculty of Medicine of Ribeirao Preto, Universidade de São Paulo, Ribeirao Preto, SP, Brazil.
authors.forename: Mariana Maloste
authors.initials: MM
authors.lastname: Butezloff
authors.affiliation: Faculty of Medicine of Ribeirao Preto, USP, Ribeirao Preto, SP, Brazil.
authors.forename: Ariane
authors.initials: A
authors.lastname: Zamarioli
authors.affiliation: Dental School of Ribeirao Preto, USP, Ribeirao Preto, SP, Brazil.
authors.forename: Graziela Bianchi
authors.initials: GB
authors.lastname: Leoni
authors.affiliation: Dental School of Ribeirao Preto, USP, Ribeirao Preto, SP, Brazil.
authors.forename: Manoel Damião
authors.initials: MD
authors.lastname: Sousa-Neto
authors.affiliation: Faculty of Medicine of Ribeirao Preto, USP, Ribeirao Preto, SP, Brazil.
authors.forename: Jose Batista
authors.initials: JB
authors.lastname: Volpon
mesh_terms: D015502:Absorptiometry, Photon; D000818:Animals; D015519:Bone Density / Q000502:physiology; D002146:Bony Callus / Q000502:physiology*; D005260:Female; D005264:Femoral Fractures / Q000209:etiology / Q000503:physiopathology / Q000628:therapy*; D017102:Fracture Healing / Q000502:physiology*; D010024:Osteoporosis / Q000503:physiopathology*; D058866:Osteoporotic Fractures / Q000209:etiology / Q000503:physiopathology / Q000628:therapy; D010052:Ovariectomy / Q000009:adverse effects*; D011897:Random Allocation; D017208:Rats, Wistar; D015203:Reproducibility of Results; D013997:Time Factors; D016896:Treatment Outcome; D014732:Vibration / Q000627:therapeutic use*