title: miR-615-5p prevents proliferation and migration through negatively regulating serine hydromethyltransferase 2 (SHMT2) in hepatocellular carcinoma.
abstract: It has been reported that miR-615-5p was upregulated in hepatocellular carcinoma (HCC) preventing both growth and migration. However, the underlying mechanism by which miR-615-5p played a role in HCC remains unknown. Here, in our present study, to investigate the mechanism of miR-615-5p, bioinformatic prediction and luciferase reporter assay were employed to ascertain the downstream target of miR-615-5p finding that the serine hydromethyltransferase 2 (SHMT2) was the direct downstream target. Knockdown or overexpression of miR-615-5p can lead to increasing or decreasing expression of SHMT2 in HCC cells. Besides, knockdown or overexpression of SHMT2 can suppress or promote both proliferation and migration of HCC cells, indicating that miR-615-5p can directly and negatively regulate the SHMT2 in HCC cells. In addition, to understand the clinicopathological significance of SHMT2 expression in HCC, immunohistochemistry was performed. It was found that SHMT2 expression was significantly associated with poor prognosis and TNM stage. Together, our results for the first time showed that miR-615-5p prevents proliferation and migration through negatively regulating SHMT2 in HCC.
authors.affiliation: Department of Surgical Oncology, Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, 155 Hanzhong Road, Nanjing, 210029, China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Wu
authors.affiliation: Department of Hepatobiliary Surgery, the Eastern Hospital of the First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510700, China., Sun Yat-sen University, Guangzhou, 510700, People's Republic of China.
authors.forename: Liang
authors.initials: L
authors.lastname: Deng
authors.affiliation: Nanjing University of Traditional Chinese Medicine, 282 Hanzhong Road, Nanjing, 210029, China.
authors.forename: Decai
authors.initials: D
authors.lastname: Tang
authors.affiliation: Nanjing University of Traditional Chinese Medicine, 282 Hanzhong Road, Nanjing, 210029, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Ying
authors.affiliation: Nanjing University of Traditional Chinese Medicine, 282 Hanzhong Road, Nanjing, 210029, China.
authors.forename: Xuequan
authors.initials: X
authors.lastname: Yao
authors.affiliation: Nanjing University of Traditional Chinese Medicine, 282 Hanzhong Road, Nanjing, 210029, China.
authors.forename: Fukun
authors.initials: F
authors.lastname: Liu
authors.affiliation: Department of General Surgery, Jiangsu Cancer Hospital, The Affiliated Cancer Hospital of Nanjing Medical University, 42 Baiziting, Nanjing, 210009, China. gs_gliang@163.com.
authors.forename: Gui
authors.initials: G
authors.lastname: Liang
mesh_terms: D000328:Adult; D000368:Aged; D001483:Base Sequence; D001665:Binding Sites; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D012696:Glycine Hydroxymethyltransferase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D053208:Kaplan-Meier Estimate; D008113:Liver Neoplasms / Q000235:genetics* / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000737:chemistry / Q000235:genetics*; D008875:Middle Aged; D060787:Neoplasm Grading; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging; D011379:Prognosis; D034622:RNA Interference; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics
chemical_list: C534344:MIRN615 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; D012696:Glycine Hydroxymethyltransferase; C520541:SHMT protein, human
keywords: Hepatocellular carcinoma (HCC); Migration; Proliferation; SHMT2; miR-615-5p