title: Spy1 participates in the proliferation and apoptosis of epithelial ovarian cancer.
abstract: This study focused on determining the role of Spy1 in human epithelial ovarian cancer (EOC). Speedy is a novel cell cycle protein capable of promoting cell proliferation. In this study, western blot and immunohistochemistrical analyses were performed to detect the expression of Spy1 in ovarian cancer. Spy1 protein levels increased with ovarian cancer grade, and Kaplan-Meier curve showed that overexpression of Spy1 was significantly correlated with reduced patient survival. In vitro, Spy1 depletion in ovarian cell lines led to reduced proliferation according to CCK8 and plate colony assays. The expression of Spy1 was positively related to pThr187-p27. Flow cytometry revealed that the reduced expression of Spy1 induced the apoptosis of the EOC cells. In summary, our findings suggested that Spy1 may be a novel independent prognostic predictor of survival for ovarian patients.
authors.affiliation: Department of Oncology, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu Province, People's Republic of China.
authors.forename: Shumin
authors.initials: S
authors.lastname: Lu
authors.affiliation: Department of Pathology, Nantong University Cancer Hospital, Nantong, 226001, Jiangsu Province, People's Republic of China.
authors.forename: Rong
authors.initials: R
authors.lastname: Liu
authors.affiliation: Department of Oncology, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu Province, People's Republic of China.
authors.forename: Min
authors.initials: M
authors.lastname: Su
authors.affiliation: Department of Pathology, Nantong University Cancer Hospital, Nantong, 226001, Jiangsu Province, People's Republic of China.
authors.forename: Yingze
authors.initials: Y
authors.lastname: Wei
authors.affiliation: Department of Pathology, Nantong University Cancer Hospital, Nantong, 226001, Jiangsu Province, People's Republic of China.
authors.forename: Shuyun
authors.initials: S
authors.lastname: Yang
authors.affiliation: Department of Pathology, Nantong University Cancer Hospital, Nantong, 226001, Jiangsu Province, People's Republic of China.
authors.forename: Song
authors.initials: S
authors.lastname: He
authors.affiliation: The Center for Reproductive Medicine, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu Province, People's Republic of China.
authors.forename: Xia
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Pathology, Nantong University Cancer Hospital, Nantong, 226001, Jiangsu Province, People's Republic of China.
authors.forename: Fulin
authors.initials: F
authors.lastname: Qiang
authors.affiliation: Department of Oncology, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu Province, People's Republic of China.
authors.forename: Chen
authors.initials: C
authors.lastname: Chen
authors.affiliation: Department of Oncology, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu Province, People's Republic of China.
authors.forename: Shuyang
authors.initials: S
authors.lastname: Zhao
authors.affiliation: Department of Oncology, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu Province, People's Republic of China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Oncology, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu Province, People's Republic of China.
authors.forename: Pan
authors.initials: P
authors.lastname: Xu
authors.affiliation: Department of Oncology, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu Province, People's Republic of China. maoguoxinnt@163.com.
authors.forename: Guoxin
authors.initials: G
authors.lastname: Mao
mesh_terms: D000328:Adult; D000368:Aged; D017209:Apoptosis / Q000235:genetics; D014408:Biomarkers, Tumor / Q000235:genetics* / Q000378:metabolism; D000077216:Carcinoma, Ovarian Epithelial; D002453:Cell Cycle / Q000235:genetics; D018797:Cell Cycle Proteins / Q000235:genetics* / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005260:Female; D015870:Gene Expression; D006801:Humans; D019394:Ki-67 Antigen / Q000235:genetics / Q000378:metabolism; D008875:Middle Aged; D060787:Neoplasm Grading; D009363:Neoplasm Proteins / Q000235:genetics* / Q000378:metabolism; D009367:Neoplasm Staging; D009375:Neoplasms, Glandular and Epithelial / Q000175:diagnosis* / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D010051:Ovarian Neoplasms / Q000175:diagnosis* / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D010053:Ovary / Q000378:metabolism / Q000473:pathology; D011379:Prognosis; D016019:Survival Analysis
chemical_list: D014408:Biomarkers, Tumor; D018797:Cell Cycle Proteins; D019394:Ki-67 Antigen; D009363:Neoplasm Proteins; C486530:SPDYA protein, human
keywords: Apoptosis; Ovarian cancer; Proliferation; Spy1