title: Athalassotoga saccharophilagen. nov., sp. nov., isolated from an acidic terrestrial hot spring, and proposal ofMesoaciditogalesord. nov. andMesoaciditogaceaefam. nov. in the phylumThermotogae.
abstract: A novel moderately thermophilic, weakly acidophilic, heterotrophic, anaerobic, short-rod bacterium having an outer sheath-like structure (toga) was isolated from a low-salt acidic terrestrial hot spring in Oku-Shiobara, Tochigi, Japan. The strain, designated NAS-01T, grew between 30 and 60 °C (optimum 55 °C), and at pH 4.5 and 7.5 (optimum pH 5.5 to 6.0) and could not grow in media with ≥ 1 % NaCl (optimum 0 % NaCl). It utilized Fe(III), thiosulfate or l-cystine as electron acceptor for growth, and yeast extract, peptone or a variety of sugars as carbon and energy sources. The major cellular fatty acid was C16 : 0, and no lipoquinone was detected. Strain NAS-01Tcontained phospholipids and glycolipids, but not aminolipids. The DNA G+C content was 41.1 mol%. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that the strain was included in the phylumThermotogae, and was closely related toMesoaciditoga lauensis. The 16S rRNA gene sequence similarity between them was 90 %, and they were deeply branched off the rest of the knownThermotogaespecies. On the basis of the phylogenetic and ecophysiological properties, strain NAS-01Trepresents a novel species of a new genus in the phylumThermotogae, for which we propose the nameAthalassotoga saccharophilagen. nov., sp. nov. The type strain of the type species is NAS-01T( = JCM 19762T= DSM 28954T). In addition, we propose a new order and family,Mesoaciditogalesord. nov. andMesoaciditogaceaefam. nov., respectively, to accommodate the novel genus and the closely related genusMesoaciditoga.
authors.affiliation: Japan Collection of Microorganisms, RIKEN BioResource Center, 3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Itoh
authors.affiliation: Japan Collection of Microorganisms, RIKEN BioResource Center, 3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan.|Graduate School of Life Sciences, Toyo University, Oura, Gunma 374-0193, Japan.
authors.forename: Masafumi
authors.initials: M
authors.lastname: Onishi
authors.affiliation: Japan Collection of Microorganisms, RIKEN BioResource Center, 3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan.
authors.forename: Shingo
authors.initials: S
authors.lastname: Kato
authors.affiliation: Japan Collection of Microorganisms, RIKEN BioResource Center, 3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan.
authors.forename: Takao
authors.initials: T
authors.lastname: Iino
authors.affiliation: Japan Collection of Microorganisms, RIKEN BioResource Center, 3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan.
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Sakamoto
authors.affiliation: Japan Collection of Microorganisms, RIKEN BioResource Center, 3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan.
authors.forename: Takuji
authors.initials: T
authors.lastname: Kudo
authors.affiliation: Graduate School of Life Sciences, Toyo University, Oura, Gunma 374-0193, Japan.
authors.forename: Tomonori
authors.initials: T
authors.lastname: Takashina
authors.affiliation: Japan Collection of Microorganisms, RIKEN BioResource Center, 3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan.
authors.forename: Moriya
authors.initials: M
authors.lastname: Ohkuma