title: Fabrication of High-Performance Magnetic Lysozyme-Imprinted Microsphere and Its NIR-Responsive Controlled Release Property.
abstract: The preparation of efficient and practical biomacromolecules imprinted polymer materials is still a challenging task because of the spatial hindrance caused by the large size of template and target molecules in the imprinting and recognition process. Herein, we provided a novel pathway to coat a NIR-light responsive lysozyme-imprinted polydopamine (PDA) layer on a fibrous SiO2 (F-SiO2) microsphere grown up from a magnetic Fe3O4 core nanoparticle. The magnetic core-shell structured lysozyme-imprinted Fe3O4@F-SiO2@PDA microspheres (MIP-lysozyme) can be easily separated by a magnet and have a high saturation adsorption capacity of lysozyme of 700 mg/g within 30 min because of the high surface area of 570 m(2)/g and the mesopore size of 12 nm of the Fe3O4@F-SiO2 support. The MIP-lysozyme microspheres also show an excellent selective adsorption of lysozyme (IF > 4). The binding thermodynamic parameters studied by ITC proves that the lysozyme should be restricted by the well-defined 3D structure of MIP-lysozyme microspheres. The MIP-lysozyme can extract lysozyme efficiently from real egg white. Owing to the efficient NIR light photothermal effect of PDA layer, the MIP-lysozyme microspheres show the controlled release property triggered by NIR laser. The released lysozyme molecules still maintain good bioactivity, which can efficiently decompose E. coli. Therefore, this work provides a novel strategy to build practical NIR-light-responsive MIPs for the extraction and application of biomacromolecules.
authors.affiliation: The USTC-Anhui Tobacco Joint Laboratory of Tobacco Chemistry, CAS Key Laboratory of Soft Matter Chemistry, Department of Polymer Science and Engineering, and ‡The USTC-Anhui Tobacco Joint Laboratory of Tobacco Chemistry, Research Center of Tobacco and Health, University of Science and Technology of China , Hefei, Anhui 230026, P. R. China.
authors.forename: Jinxing
authors.initials: J
authors.lastname: Chen
authors.affiliation: The USTC-Anhui Tobacco Joint Laboratory of Tobacco Chemistry, CAS Key Laboratory of Soft Matter Chemistry, Department of Polymer Science and Engineering, and ‡The USTC-Anhui Tobacco Joint Laboratory of Tobacco Chemistry, Research Center of Tobacco and Health, University of Science and Technology of China , Hefei, Anhui 230026, P. R. China.
authors.forename: Shan
authors.initials: S
authors.lastname: Lei
authors.affiliation: The USTC-Anhui Tobacco Joint Laboratory of Tobacco Chemistry, CAS Key Laboratory of Soft Matter Chemistry, Department of Polymer Science and Engineering, and ‡The USTC-Anhui Tobacco Joint Laboratory of Tobacco Chemistry, Research Center of Tobacco and Health, University of Science and Technology of China , Hefei, Anhui 230026, P. R. China.
authors.forename: Yunyun
authors.initials: Y
authors.lastname: Xie
authors.affiliation: The USTC-Anhui Tobacco Joint Laboratory of Tobacco Chemistry, CAS Key Laboratory of Soft Matter Chemistry, Department of Polymer Science and Engineering, and ‡The USTC-Anhui Tobacco Joint Laboratory of Tobacco Chemistry, Research Center of Tobacco and Health, University of Science and Technology of China , Hefei, Anhui 230026, P. R. China.
authors.forename: Mozhen
authors.initials: M
authors.lastname: Wang
authors.affiliation: The USTC-Anhui Tobacco Joint Laboratory of Tobacco Chemistry, CAS Key Laboratory of Soft Matter Chemistry, Department of Polymer Science and Engineering, and ‡The USTC-Anhui Tobacco Joint Laboratory of Tobacco Chemistry, Research Center of Tobacco and Health, University of Science and Technology of China , Hefei, Anhui 230026, P. R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
authors.affiliation: The USTC-Anhui Tobacco Joint Laboratory of Tobacco Chemistry, CAS Key Laboratory of Soft Matter Chemistry, Department of Polymer Science and Engineering, and ‡The USTC-Anhui Tobacco Joint Laboratory of Tobacco Chemistry, Research Center of Tobacco and Health, University of Science and Technology of China , Hefei, Anhui 230026, P. R. China.
authors.forename: Xuewu
authors.initials: X
authors.lastname: Ge
mesh_terms: D003692:Delayed-Action Preparations / Q000737:chemistry* / Q000494:pharmacology / Q000528:radiation effects; D004926:Escherichia coli / Q000187:drug effects; D007211:Indoles / Q000737:chemistry; D007259:Infrared Rays; D058185:Magnetite Nanoparticles / Q000737:chemistry; D008863:Microspheres; D054802:Molecular Imprinting; D009113:Muramidase / Q000737:chemistry* / Q000494:pharmacology; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology / Q000528:radiation effects
chemical_list: D003692:Delayed-Action Preparations; D007211:Indoles; D058185:Magnetite Nanoparticles; D011108:Polymers; C568283:polydopamine; D009113:Muramidase
keywords: Fe3O4 nanoparticles; NIR-light; controlled release; fibrous SiO2; lysozyme; polydopamine; protein imprinting