title: DNA-directed spatial assembly of photosynthetic light-harvesting proteins.
abstract: This manuscript describes the surface immobilization of a light-harvesting complex to prescribed locations directed by the sequence-selective recognition of duplex DNA. An engineered light-harvesting complex (RC-LH1) derived from Rhodopseudomonas (Rps.) palustris containing the zinc finger (ZF) domain zif268 was prepared. The zif268 domain directed the binding of zfRC-LH1 to target double-stranded DNA sequences both in solution and when immobilized on lithographically defined micro-patterns. Excitation energy transfer from the carotenoids to the bacteriochlorophyll pigments within zfRC-LH1 confirmed that the functional and structural integrity of the complex is retained after surface immobilization.
authors.affiliation: Molecular Cell and Systems Biology, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow G12 8TA, UK. Richard.Cogdell@glasgow.ac.uk.
authors.forename: Sarah L
authors.initials: SL
authors.lastname: Henry
authors.forename: Jamie M
authors.initials: JM
authors.lastname: Withers
authors.forename: Ishwar
authors.initials: I
authors.lastname: Singh
authors.forename: Jonathan M
authors.initials: JM
authors.lastname: Cooper
authors.forename: Alasdair W
authors.initials: AW
authors.lastname: Clark
authors.forename: Glenn A
authors.initials: GA
authors.lastname: Burley
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Cogdell
mesh_terms: D004247:DNA / Q000378:metabolism*; D004735:Energy Transfer; D045342:Light-Harvesting Protein Complexes / Q000737:chemistry / Q000378:metabolism*; D008958:Models, Molecular; D010788:Photosynthesis; D012241:Rhodopseudomonas / Q000737:chemistry* / Q000378:metabolism
chemical_list: D045342:Light-Harvesting Protein Complexes; D004247:DNA