title: The trout farm effect on Dinocras megacephala (Plecoptera: Perlidae) larvae: Antioxidative defense.
abstract: Trout production represents a major agricultural activity in Serbia. Organic compounds are drained into the environment, usually without previous sedimentation, thus affecting the stream biota. Considering that biological monitoring is commonly based on aquatic macroinvertebrates as target organisms, the authors used larvae of Dinocras megacephala to estimate trout farm effects on the mass of the larvae and their antioxidative defense in pollution stress conditions. Four locations were chosen along the channel of the Raška River, 2 upstream (L1, L2) and 2 downstream (L3, L4) from the trout farm outlet. Basic physical and chemical water parameters were measured. Superoxide dismutase (SOD) and catalase (CAT) activity changes were analyzed to determine the level of oxidative stress caused by the increase of organic compounds originating from the trout farm. Dissolved oxygen concentration decreased from the upstream to downstream locations. Furthermore, the concentration of ionized ammonia was almost 10 times higher at the downstream locations than at the upstream locations. Larval mass, as well as CAT activity, was significantly higher at L3 compared with the other 3 locations. Activity of SOD was significantly higher at L3 than at L1. The results indicate that higher concentrations of organic compounds from the trout farm induce clear changes in the status of the antioxidant defense of D. megacephala larvae. Environ Toxicol Chem 2016;35:1775-1782. © 2015 SETAC.
authors.affiliation: Department of Biomedical Sciences, State University of Novi Pazar, Novi Pazar, Serbia.
authors.forename: Dejan
authors.initials: D
authors.lastname: Mirčić
authors.affiliation: Department of Invertebrate Zoology and Entomology, University of Belgrade, Belgrade, Serbia.
authors.forename: Katarina
authors.initials: K
authors.lastname: Stojanović
authors.affiliation: Department of Invertebrate Zoology and Entomology, University of Belgrade, Belgrade, Serbia.
authors.forename: Ivana
authors.initials: I
authors.lastname: Živić
authors.affiliation: Institute for Biological Research "Siniša Stanković," Department of Insect Physiology and Biochemistry, University of Belgrade, Belgrade, Serbia.
authors.forename: Dajana
authors.initials: D
authors.lastname: Todorović
authors.affiliation: Department of Biomedical Sciences, State University of Novi Pazar, Novi Pazar, Serbia.
authors.forename: Dalibor
authors.initials: D
authors.lastname: Stojanović
authors.affiliation: Department of Biomedical Sciences, State University of Novi Pazar, Novi Pazar, Serbia.
authors.forename: Zana
authors.initials: Z
authors.lastname: Dolićanin
authors.affiliation: Institute for Biological Research "Siniša Stanković," Department of Insect Physiology and Biochemistry, University of Belgrade, Belgrade, Serbia.
authors.forename: Vesna
authors.initials: V
authors.lastname: Perić-Mataruga
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D004784:Environmental Monitoring / Q000379:methods*; D005398:Fisheries; D007313:Insecta / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D007814:Larva; D018384:Oxidative Stress / Q000187:drug effects; D045483:Rivers / Q000737:chemistry*; D055771:Serbia; D013482:Superoxide Dismutase / Q000378:metabolism; D014337:Trout / Q000254:growth & development*; D014876:Water Pollution / Q000009:adverse effects / Q000032:analysis
chemical_list: D000975:Antioxidants; D002374:Catalase; D013482:Superoxide Dismutase
keywords: Dinocras megacephala; Ionized ammonia; Organic pollution; Oxidative stress; Trout farm