title: The dengue virus non-structural protein 1 (NS1) is secreted efficiently from infected mosquito cells.
abstract: Dengue virus NS1 is a glycoprotein of 46-50kDa which associates as a dimer to internal and cytoplasmic membranes and is also secreted, as a hexamer, to the extracellular milieu. However, the notion exist that NS1 is secreted only from infected vertebrate and not mosquito cells. In this work, evidence is presented showing that NS1 is secreted efficiently by infected mosquito cells. NS1 was detected in cell supernatants starting at 6hpi with a continuous concentration increase up to 24hpi. Nevertheless, cell viability showed an average cell survival of 97%. At variance with observations with vertebrate cells, NS1 does not seems to associate with the cytoplasmic membrane of insect cells. Finally, evidence is presented indicating that NS1 is secreted from insect cells as a barrel-shaped hexamer. These findings provide new insights into the biology of NS1 and open questions about the role of secreted NS1 in the vector mosquito.
authors.affiliation: Department of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies (CINVESTAV-IPN), Mexico City, D.F., Mexico.
authors.forename: Ana C
authors.initials: AC
authors.lastname: Alcalá
authors.affiliation: Department of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies (CINVESTAV-IPN), Mexico City, D.F., Mexico.
authors.forename: Fernando
authors.initials: F
authors.lastname: Medina
authors.affiliation: Department of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies (CINVESTAV-IPN), Mexico City, D.F., Mexico.
authors.forename: Arturo
authors.initials: A
authors.lastname: González-Robles
authors.affiliation: Department of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies (CINVESTAV-IPN), Mexico City, D.F., Mexico.
authors.forename: Lizbeth
authors.initials: L
authors.lastname: Salazar-Villatoro
authors.affiliation: Department of Physics, Center for Research and Advanced Studies (CINVESTAV-IPN), Mexico City, D.F., Mexico.
authors.forename: Rogelio J
authors.initials: RJ
authors.lastname: Fragoso-Soriano
authors.affiliation: Department of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies (CINVESTAV-IPN), Mexico City, D.F., Mexico.
authors.forename: Carlos
authors.initials: C
authors.lastname: Vásquez
authors.affiliation: Department of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies (CINVESTAV-IPN), Mexico City, D.F., Mexico.
authors.forename: Margot
authors.initials: M
authors.lastname: Cervantes-Salazar
authors.affiliation: Department of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies (CINVESTAV-IPN), Mexico City, D.F., Mexico.
authors.forename: Rosa M
authors.initials: RM
authors.lastname: Del Angel
authors.affiliation: Department of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies (CINVESTAV-IPN), Mexico City, D.F., Mexico. Electronic address: jludert@cinvestav.mx.
authors.forename: Juan E
authors.initials: JE
authors.lastname: Ludert
mesh_terms: D000818:Animals; D002460:Cell Line; D009033:Culicidae / Q000821:virology*; D003470:Culture Media / Q000737:chemistry; D003716:Dengue Virus / Q000502:physiology*; D013997:Time Factors; D017361:Viral Nonstructural Proteins / Q000378:metabolism*
chemical_list: D003470:Culture Media; C079340:NS1 protein, Dengue virus type 2; D017361:Viral Nonstructural Proteins
keywords: Dengue virus; Flavivirus; Mosquito cells; NS1 protein