title: Genetic Immunization With In Vivo Dendritic Cell-targeting Liposomal DNA Vaccine Carrier Induces Long-lasting Antitumor Immune Response.
abstract: A major limiting factor retarding the clinical success of dendritic cell (DC)-based genetic immunizations (DNA vaccination) is the scarcity of biologically safe and effective carrier systems for targeting the antigen-encoded DNA vaccines to DCs under in vivo settings. Herein, we report on a potent, mannose receptor selective in vivo DC-targeting liposomes of a novel cationic amphiphile with mannose-mimicking shikimoyl head-group. Flow cytometric experiments with cells isolated from draining lymph nodes of mice s.c. immunized with lipoplexes of pGFP plasmid (model DNA vaccine) using anti-CD11c antibody-labeled magnetic beads revealed in vivo DC-targeting properties of the presently described liposomal DNA vaccine carrier. Importantly, s.c. immunizations of mice with electrostatic complex of the in vivo DC-targeting liposome and melanoma antigen-encoded DNA vaccine (p-CMV-MART1) induced long-lasting antimelanoma immune response (100 days post melanoma tumor challenge) with remarkable memory response (more than 6 months after the second tumor challenge). The presently described direct in vivo DC-targeting liposomal DNA vaccine carrier is expected to find future exploitations toward designing effective vaccines for various infectious diseases and cancers.
authors.affiliation: Biomaterials Group, CSIR - Indian Institute of Chemical Technology, Hyderabad, Telangana, India.
authors.forename: Arup
authors.initials: A
authors.lastname: Garu
authors.affiliation: Biomaterials Group, CSIR - Indian Institute of Chemical Technology, Hyderabad, Telangana, India; Academy of Scientific and Innovative Research (AcSIR), Chennai, Tamil Nadu, India.
authors.forename: Gopikrishna
authors.initials: G
authors.lastname: Moku
authors.affiliation: Biomaterials Group, CSIR - Indian Institute of Chemical Technology, Hyderabad, Telangana, India; Academy of Scientific and Innovative Research (AcSIR), Chennai, Tamil Nadu, India.
authors.forename: Suresh Kumar
authors.initials: SK
authors.lastname: Gulla
authors.affiliation: Biomaterials Group, CSIR - Indian Institute of Chemical Technology, Hyderabad, Telangana, India; Academy of Scientific and Innovative Research (AcSIR), Chennai, Tamil Nadu, India. Electronic address: arabinda@iict.res.in.
authors.forename: Arabinda
authors.initials: A
authors.lastname: Chaudhuri
mesh_terms: D000818:Animals; D032701:Biomimetics; D039521:CD11c Antigen / Q000235:genetics; D019496:Cancer Vaccines / Q000008:administration & dosage* / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology*; D005260:Female; D016233:Immunotherapy, Active; D008081:Liposomes; D051379:Mice; D009369:Neoplasms / Q000188:drug therapy* / Q000276:immunology; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000276:immunology
chemical_list: D039521:CD11c Antigen; D019496:Cancer Vaccines; D008081:Liposomes; D019444:Vaccines, DNA