title: Analysis of 17 STR data on 5362 southern Portuguese individuals-an update on reference database.
abstract: The main objective of this work consisted of the updating of allele frequencies and other relevant forensic parameters for the 17 autosomal STR loci provided by the combination of the two types of kits used routinely in our laboratory casework: AmpF/STR Identifiler(®) and the Powerplex(®) 16 Systems. This aim was of significant importance, given that the last study on these kits within the southern Portuguese population dates back to 2006, and, as a consequence, it was necessary to correct the deviation caused by population evolution over the last ten years so that they might be better applied to our forensic casework. For this reason genetic data from 5362 unrelated Caucasian Portuguese individuals from the south of Portugal who were involved in paternity testing casework from 2005 to 2014 was used. Of all the markers, TPOX proved to be the least polymorphic, and Penta E the most. Secondly, this up-to-date southern Portuguese population was compared not only with the northern and central Portuguese populations, but also with that of southern Portugal in 2006, along with populations from Spain, Italy, Greece, Romania, Morocco, Angola and Korea in order to infer information about the relatedness of these respective populations, and the variation of the southern Portuguese population over time.
authors.affiliation: National Institute of Legal Medicine and Forensic Sciences, Portugal; University of Lisbon, Faculty of Medicine, Portugal. Electronic address: raquel.csiilva@gmail.com.
authors.forename: Raquel
authors.initials: R
authors.lastname: Cabezas Silva
authors.affiliation: National Institute of Legal Medicine and Forensic Sciences, Portugal; CENCIFOR-Forensic Sciences Centre, Portugal. Electronic address: tribeiro@dlinml.mj.pt.
authors.forename: Teresa
authors.initials: T
authors.lastname: Ribeiro
authors.affiliation: National Institute of Legal Medicine and Forensic Sciences, Portugal. Electronic address: ilucas@dlinml.mj.pt.
authors.forename: Isabel
authors.initials: I
authors.lastname: Lucas
authors.affiliation: National Institute of Legal Medicine and Forensic Sciences, Portugal; CENCIFOR-Forensic Sciences Centre, Portugal. Electronic address: mariajoao.porto@dcinml.mj.pt.
authors.forename: Maria João
authors.initials: MJ
authors.lastname: Porto
authors.affiliation: National Institute of Legal Medicine and Forensic Sciences, Portugal; CENCIFOR-Forensic Sciences Centre, Portugal; University of Lisbon, Faculty of Medicine, Portugal. Electronic address: jsantos@dlinml.mj.pt.
authors.forename: Jorge
authors.initials: J
authors.lastname: Costa Santos
authors.affiliation: National Institute of Legal Medicine and Forensic Sciences, Portugal; University of Lisbon, Faculty of Sciences, Portugal; CENCIFOR-Forensic Sciences Centre, Portugal; CESAM-Centre for Environmental and Marine Studies, Lisboa, Portugal. Electronic address: pdario@dlinml.mj.pt.
authors.forename: Paulo
authors.initials: P
authors.lastname: Dario
mesh_terms: D030541:Databases, Genetic; D053803:Forensic Genetics / Q000379:methods*; D005787:Gene Frequency; D014644:Genetic Variation; D005828:Genetics, Population / Q000379:methods*; D006801:Humans; D018895:Microsatellite Repeats; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011174:Portugal; D012016:Reference Values
keywords: Identifiler(®); Population data update; Population genetics; Portugal; Powerplex(®) 16