title: Direct detection of lipid A on intact Gram-negative bacteria by MALDI-TOF mass spectrometry.
abstract: The purification and characterization of Gram-negative bacterial lipid A is tedious and time-consuming. Herein we report a rapid and sensitive method to identify lipid A directly on intact bacteria without any chemical treatment or purification, using an atypical solvent system to solubilize the matrix combined with MALDI-TOF mass spectrometry.
authors.affiliation: MRC Centre for Molecular Bacteriology and Infection, Department of Life Sciences, Faculty of Natural Sciences, Imperial College London, London, UK. Electronic address: g.larrouy-maumus@imperial.ac.uk.
authors.forename: Gerald
authors.initials: G
authors.lastname: Larrouy-Maumus
authors.affiliation: MRC Centre for Molecular Bacteriology and Infection, Department of Life Sciences, Faculty of Natural Sciences, Imperial College London, London, UK.
authors.forename: Abigail
authors.initials: A
authors.lastname: Clements
authors.affiliation: MRC Centre for Molecular Bacteriology and Infection, Department of Life Sciences, Faculty of Natural Sciences, Imperial College London, London, UK.
authors.forename: Alain
authors.initials: A
authors.lastname: Filloux
authors.affiliation: MRC Centre for Molecular Bacteriology and Infection, Department of Life Sciences, Faculty of Natural Sciences, Imperial College London, London, UK.
authors.forename: Ronan R
authors.initials: RR
authors.lastname: McCarthy
authors.affiliation: MRC Centre for Molecular Bacteriology and Infection, Department of Medicine, Imperial College London, London, UK.
authors.forename: Serge
authors.initials: S
authors.lastname: Mostowy
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods; D004731:Endotoxins / Q000032:analysis / Q000737:chemistry; D006090:Gram-Negative Bacteria / Q000737:chemistry* / Q000302:isolation & purification; D008050:Lipid A / Q000032:analysis / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013045:Species Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation / Q000379:methods*
chemical_list: D004731:Endotoxins; D008050:Lipid A
keywords: Endotoxins; Gram-negative bacteria; Mass spectrometry