title: Effect of ultrasonication on the fibril-formation and gel properties of collagen from grass carp skin.
abstract: Controlling the fibril-formation process of collagen in vitro to fabricate novel biomaterials is a new area in the field of collagen research. This study aimed to determine the effect of ultrasonication on collagen fibril formation and the properties of the resulting collagen gels. Native collagen, extracted from the skin of grass carp, self-assembled under ultrasonic conditions (at different ultrasonic power and duration). The self-assembly kinetics, fibrillar morphology, and physical and cell growth-promoting properties of the collagen gels were analyzed and compared. The results showed that the self-assembly rate of collagen was increased by ultrasonication at the nucleation stage. The resulting fibrils exhibited smaller diameters and D-periodicity lengths than that of the untreated collagen samples (p<0.05). The viscoelasticity and textural properties of collagen gels also changed after ultrasonication at the nucleation stage. Texture profile analysis and cell proliferation assays showed that ultrasonication produced softer collagen gel colloids, which were more suitable for cell proliferation than the untreated collagen gels.
authors.affiliation: College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, Hubei, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Department of Chemical and Environmental Engineering, Wuhan Polytechnic University, Wuhan, Hubei, China. Electronic address: wanghaibo@whpu.edu.cn.
authors.forename: Haibo
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Chemical and Environmental Engineering, Wuhan Polytechnic University, Wuhan, Hubei, China.
authors.forename: Mingxia
authors.initials: M
authors.lastname: Deng
authors.affiliation: College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, Hubei, China.
authors.forename: Zhongwen
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Chemical and Environmental Engineering, Wuhan Polytechnic University, Wuhan, Hubei, China.
authors.forename: Juntao
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Tongji Medical Collage, Huzhong University of Science and Technology, Wuhan, Hubei, China.
authors.forename: Haiyin
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Chemical and Environmental Engineering, Wuhan Polytechnic University, Wuhan, Hubei, China.
authors.forename: Hanjun
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000528:radiation effects; D002347:Carps; D003094:Collagen / Q000737:chemistry* / Q000378:metabolism* / Q000528:radiation effects / Q000648:ultrastructure; D004548:Elasticity; D005782:Gels; D051379:Mice; D041681:NIH 3T3 Cells; D012867:Skin / Q000737:chemistry* / Q000528:radiation effects; D013010:Sonication; D014783:Viscosity
chemical_list: D001672:Biocompatible Materials; D005782:Gels; D003094:Collagen
keywords: Collagen; Collagen gels; Fibril formation; Properties; Ultrasonic treatment