title: Human papillomavirus detection in Corrientes, Argentina: High prevalence of type 58 and its phylodynamics.
abstract: Human papillomavirus (HPV) has the highest mortality rate due to cervical cancer in Northeastern Argentina. The aim of this work was to detect and characterize HPV in samples from the Province of Corrientes, Argentina. HPV detection and typing was performed using PCR-RFLP on samples with different cervical lesions (n=255). Seventeen viruses typified as HPV-58 were sequenced (E6 and E7 genes) and mutations were analyzed. HPV DNA was detected in 56.1% of the cervical lesions (143/255). Twenty-two different HPV types were detected. The type most frequently found among the total number of samples and HPV-positive samples was HPV-16 (14.5% and 25.9%, respectively), followed by HPV-58 (8.2%/14.7%, respectively), which is also considered a high-risk viral type. Increased severity of the cytological status was associated with greater rates of HPV detection and, especially, with the detection of greater rates of high-risk types. In addition, the evolutionary dynamics of the alpha-9 species group and HPV-58 was studied. All HPV-58 viruses reported in this work belonged to lineage A, sublineage A2. The phylodynamic analysis indicated that diversification of main groups within lineage A might have accompanied or preceded human migrations across the globe. Given that the most prevalent viruses found belonged to high-risk HPV types, some concerns might arise about the extent of cross protection of the vaccines against the types not included in their design.
authors.affiliation: Instituto de Medicina Regional, Universidad Nacional del Nordeste, Av Las Heras 727, Resistencia 3500, Chaco, Argentina.
authors.forename: Héctor M
authors.initials: HM
authors.lastname: Marín
authors.affiliation: Cátedra de Virología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, 4° piso, Ciudad Autónoma de Buenos Aires 1113, Argentina; CONICET, Av. Rivadavia 1917, Ciudad Autónoma de Buenos Aires 1033, Argentina.
authors.forename: Carolina
authors.initials: C
authors.lastname: Torres
authors.affiliation: Cátedra de Virología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, 4° piso, Ciudad Autónoma de Buenos Aires 1113, Argentina.
authors.forename: Gerardo D
authors.initials: GD
authors.lastname: Deluca
authors.affiliation: Cátedra de Virología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, 4° piso, Ciudad Autónoma de Buenos Aires 1113, Argentina; CONICET, Av. Rivadavia 1917, Ciudad Autónoma de Buenos Aires 1033, Argentina. Electronic address: vmbayed@ffyb.uba.ar.
authors.forename: Viviana A
authors.initials: VA
authors.lastname: Mbayed
mesh_terms: D000293:Adolescent; D000328:Adult; D001118:Argentina; D005260:Female; D006801:Humans; D008875:Middle Aged; D027383:Papillomaviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000821:virology*; D010802:Phylogeny; D015995:Prevalence; D002583:Uterine Cervical Neoplasms / Q000821:virology*; D055815:Young Adult
keywords: Argentina; E6; E7; Filodinámica; HPV-58; Phylodynamics