title: Isolation, characterization, and radiation protection of Sipunculus nudus L. polysaccharide.
abstract: Sipunculus nudus Linnaeus polysaccharide (SNP) was purified from S. nudus L. via NaOH extraction, trichloroacetic acid deproteination, DEAE-cellulose 52 and Sephacryl S-300 chromatography. The monosaccharide analysis and molecular weight was detected with HPLC. FT-IR, 1H spectrum and 13C NMR spectrum were performed to detect the chemical characteristics. The antioxidant activity was assayed in vitro. The radiation protection effects were detected on mice. The results showed that SNP was composed of mannose, rhamnose, galacturonic acid, glucose, arabinose and fucose, and the average molecular weight was 680 kDa. Above the concentration of 10 mg/mL, SNP showed powerful scavenging activity on hydroxyl radical. In the animals irradiated with a 7.5 Gy γ-rays, the 90 mg/kg and the 270 mg/kg SNP groups survived significantly longer than the radiation control group. In the animals irradiated with a 4.0 Gy γ-rays, SNP showed significant protection effect. The contents of DNA in bone marrow cells were significantly increased by SNP treatment, and the micronucleus rates of 30 mg/kg and 270 mg/kg SNP groups were decrease significantly compared to the radiation control group. These findings suggest that SNP possesses marked antioxidant and bone marrow damage protection capacity which play important roles in the prevention of radiation damage.
authors.affiliation: The PLA Key Laboratory of Biological Effect and Medical Protection on Naval Vessel Special Environment, Naval Medical Research Institute, Shanghai 200433, China; College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China.
authors.forename: Na
authors.initials: N
authors.lastname: Li
authors.affiliation: The PLA Key Laboratory of Biological Effect and Medical Protection on Naval Vessel Special Environment, Naval Medical Research Institute, Shanghai 200433, China. Electronic address: xianrong_sh@163.com.
authors.forename: Xianrong
authors.initials: X
authors.lastname: Shen
authors.affiliation: The PLA Key Laboratory of Biological Effect and Medical Protection on Naval Vessel Special Environment, Naval Medical Research Institute, Shanghai 200433, China.
authors.forename: Yuming
authors.initials: Y
authors.lastname: Liu
authors.affiliation: The PLA Key Laboratory of Biological Effect and Medical Protection on Naval Vessel Special Environment, Naval Medical Research Institute, Shanghai 200433, China; College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China.
authors.forename: Junling
authors.initials: J
authors.lastname: Zhang
authors.affiliation: The PLA Key Laboratory of Biological Effect and Medical Protection on Naval Vessel Special Environment, Naval Medical Research Institute, Shanghai 200433, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: He
authors.affiliation: The PLA Key Laboratory of Biological Effect and Medical Protection on Naval Vessel Special Environment, Naval Medical Research Institute, Shanghai 200433, China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Liu
authors.affiliation: The PLA Key Laboratory of Biological Effect and Medical Protection on Naval Vessel Special Environment, Naval Medical Research Institute, Shanghai 200433, China.
authors.forename: Dingwen
authors.initials: D
authors.lastname: Jiang
authors.affiliation: The PLA Key Laboratory of Biological Effect and Medical Protection on Naval Vessel Special Environment, Naval Medical Research Institute, Shanghai 200433, China; College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zong
authors.affiliation: The PLA Key Laboratory of Biological Effect and Medical Protection on Naval Vessel Special Environment, Naval Medical Research Institute, Shanghai 200433, China; Metabolism Laboratory of Traditional Chinese Medicine, Zhejiang Chinese Medical University, Hangzhou 310053, China.
authors.forename: Jiamei
authors.initials: J
authors.lastname: Li
authors.affiliation: The PLA Key Laboratory of Biological Effect and Medical Protection on Naval Vessel Special Environment, Naval Medical Research Institute, Shanghai 200433, China.
authors.forename: Dengyong
authors.initials: D
authors.lastname: Hou
authors.affiliation: The PLA Key Laboratory of Biological Effect and Medical Protection on Naval Vessel Special Environment, Naval Medical Research Institute, Shanghai 200433, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.affiliation: The PLA Key Laboratory of Biological Effect and Medical Protection on Naval Vessel Special Environment, Naval Medical Research Institute, Shanghai 200433, China.
authors.forename: Qingrong
authors.initials: Q
authors.lastname: Wang
authors.affiliation: The PLA Key Laboratory of Biological Effect and Medical Protection on Naval Vessel Special Environment, Naval Medical Research Institute, Shanghai 200433, China.
authors.forename: Qun
authors.initials: Q
authors.lastname: Luo
authors.affiliation: The PLA Key Laboratory of Biological Effect and Medical Protection on Naval Vessel Special Environment, Naval Medical Research Institute, Shanghai 200433, China.
authors.forename: Kexian
authors.initials: K
authors.lastname: Li
mesh_terms: D000818:Animals; D059001:Aquatic Organisms / Q000737:chemistry*; D001854:Bone Marrow Cells / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D004247:DNA / Q000378:metabolism; D004307:Dose-Response Relationship, Radiation; D016166:Free Radical Scavengers / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D005979:Glutathione Peroxidase / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D015162:Micronucleus Tests; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D011837:Radiation-Protective Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D016166:Free Radical Scavengers; D011134:Polysaccharides; D011837:Radiation-Protective Agents; D008315:Malondialdehyde; D004247:DNA; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase
keywords: Bone marrow cell; Hydroxyl radical; Micronucleus rate; Polysaccharide; Radiation protection; Sipunculus nudus L.