title: Bioinformatic prediction of the antigenic epitopes of recombinant ferritin of Echinococcus granulosus.
abstract: Echinococcosis is a zoonotic parasitic disease affecting humans and other mammals, which is mainly caused Echinococcus at larval stages. It is predominantly endemic in Chinese pasture regions, including Xinjiang, Qinghai, Gansu and Ningxia. The aim of the present study was to predict the T‑ and B‑combined epitopes of Echinococcus granulosus (Eg). ferritin, and to analyze its secondary structure using online software. Prediction of the T‑ and B‑combined epitopes of Eg. ferritin was performed using IEDB, SYFPEITHI and LEPS software, which are used to identify common areas of T‑ and B‑cells. The results of the present study identified several potential antigenic epitopes of Eg. ferritin, including seven B‑cell antigen epitope amino acid sequences with high values: 8‑16, 54‑61, 70‑75, 80‑90, 103‑109, 117‑124 and 167‑173; and four T‑cell antigen epitope amino acid sequences with high values: 85‑93, 105‑113, 133‑141 and 157‑165. Furthermore, a combined epitope region comprising an 105‑109 amino acid sequence was identified. In conclusion, using bioinformatic methods, the present study confirmed the existence of Eg. ferritin on four T‑cell antigen epitopes, seven B‑cell antigen epitopes, and one T‑ and B‑combined epitope region. These findings provide significant information for further investigation of the antigenicity of Eg. ferritin and the development of highly efficient epitope vaccines.
authors.affiliation: State Key Laboratory Incubation Base of Xinjiang Major Diseases Research (2010DS890294) and Xinjiang Key Laboratory of Echinococcosis, First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830000, P.R. China.
authors.forename: Xuelei
authors.initials: X
authors.lastname: Liu
authors.affiliation: State Key Laboratory Incubation Base of Xinjiang Major Diseases Research (2010DS890294) and Xinjiang Key Laboratory of Echinococcosis, First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830000, P.R. China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhao
authors.affiliation: State Key Laboratory Incubation Base of Xinjiang Major Diseases Research (2010DS890294) and Xinjiang Key Laboratory of Echinococcosis, First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830000, P.R. China.
authors.forename: Wenyan
authors.initials: W
authors.lastname: Cao
authors.affiliation: State Key Laboratory Incubation Base of Xinjiang Major Diseases Research (2010DS890294) and Xinjiang Key Laboratory of Echinococcosis, First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830000, P.R. China.
authors.forename: Yumei
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Immunology, College of Basic Medicine of Xinjiang Medical University, Urumqi, Xinjiang 830011, P.R. China.
authors.forename: Chuntao
authors.initials: C
authors.lastname: Zhang
authors.affiliation: State Key Laboratory Incubation Base of Xinjiang Major Diseases Research (2010DS890294) and Xinjiang Key Laboratory of Echinococcosis, First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830000, P.R. China.
authors.forename: Xi
authors.initials: X
authors.lastname: Lan
authors.affiliation: Department of Immunology, College of Basic Medicine of Xinjiang Medical University, Urumqi, Xinjiang 830011, P.R. China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Peng
authors.affiliation: State Key Laboratory Incubation Base of Xinjiang Major Diseases Research (2010DS890294) and Xinjiang Key Laboratory of Echinococcosis, First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830000, P.R. China.
authors.forename: Hao
authors.initials: H
authors.lastname: Wen
authors.affiliation: State Key Laboratory Incubation Base of Xinjiang Major Diseases Research (2010DS890294) and Xinjiang Key Laboratory of Echinococcosis, First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830000, P.R. China.
authors.forename: Jianbing
authors.initials: J
authors.lastname: Ding
authors.affiliation: State Key Laboratory Incubation Base of Xinjiang Major Diseases Research (2010DS890294) and Xinjiang Key Laboratory of Echinococcosis, First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830000, P.R. China.
authors.forename: Xiumin
authors.initials: X
authors.lastname: Ma
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D003001:Cloning, Molecular; D019295:Computational Biology / Q000379:methods*; D048209:Echinococcus granulosus / Q000276:immunology*; D004587:Electrophoresis, Agar Gel; D000939:Epitopes / Q000276:immunology*; D018985:Epitopes, B-Lymphocyte / Q000276:immunology; D005293:Ferritins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D015395:Histocompatibility Antigens Class I / Q000276:immunology; D008958:Models, Molecular; D008969:Molecular Sequence Data; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D018510:RNA, Helminth / Q000302:isolation & purification / Q000378:metabolism; D011994:Recombinant Proteins / Q000276:immunology*; D012756:Sheep
chemical_list: D000939:Epitopes; D018985:Epitopes, B-Lymphocyte; D015395:Histocompatibility Antigens Class I; D018510:RNA, Helminth; D011994:Recombinant Proteins; D005293:Ferritins