title: Development of a Bladder Bioreactor for Tissue Engineering in Urology.
abstract: A urinary bladder bioreactor was constructed to replicate physiological bladder dynamics. A cyclical low-delivery pressure regulator mimicked filling pressures of the human bladder. Cell growth was evaluated by culturing human urothelial cells (UCs) on porcine extracellular matrix scaffolds (ECMs) in the bioreactor and in static growth conditions for 5 consecutive days. UC proliferation was compared with quantitative viability indicators and by fluorescent markers for intracellular esterase activity and plasma membrane integrity. Scaffold integrity was characterized with scanning electron microscopy and 4,6-diamidino-2-phenylindole staining.
authors.affiliation: Department of Mechanical and Aeronautical Engineering and MSSI, Centre for Applied Biomedical Engineering Research, University of Limerick, Limerick, Ireland. nialldavis@rcsi.ie.
authors.forename: Niall F
authors.initials: NF
authors.lastname: Davis
authors.affiliation: Department of Mechanical and Aeronautical Engineering and MSSI, Centre for Applied Biomedical Engineering Research, University of Limerick, Limerick, Ireland.|Institute for Bioengineering, School of Engineering, The University of Edinburgh, Edinburgh, EH93JL, UK.
authors.forename: Anthony
authors.initials: A
authors.lastname: Callanan
mesh_terms: D000818:Animals; D019149:Bioreactors; D002460:Cell Line; D049109:Cell Proliferation; D002470:Cell Survival; D004867:Equipment Design; D005109:Extracellular Matrix / Q000737:chemistry; D006801:Humans; D044968:Regenerative Medicine / Q000295:instrumentation / Q000379:methods*; D013552:Swine; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D001743:Urinary Bladder / Q000166:cytology*; D019459:Urothelium / Q000166:cytology*
keywords: Bladder bioreactor; Regenerative medicine; Tissue-engineering; Urothelial cells