title: Polylactide-based microspheres prepared using solid-state copolymerized chitosan and d,l-lactide.
abstract: Amphiphilic chitosan-g-poly(d,l-lactide) copolymers have been manufactured via solid-state mechanochemical copolymerization and tailored to design polyester-based microspheres for tissue engineering. A single-step solid-state reactive blending (SSRB) using low-temperature co-extrusion has been used to prepare these copolymers. These materials have been valorized to stabilize microspheres processed by an oil/water emulsion evaporation technique. Introduction of the copolymers either in water or in the oil phase of the emulsion allowed to replace a non-degradable emulsifier typically used for microparticle preparation. To enhance cell adhesion, these copolymers were also tailored to bring amino-saccharide positively charged segments to the microbead surface. Size distribution, surface morphology, and total microparticle yield have been studied and optimized as a function of the copolymer composition.
authors.affiliation: Enikolopov Institute of Synthetic Polymer Materials, Russian Academy of Sciences, Profsoyuznaya Str., 70, 117393 Moscow, Russia. Electronic address: detans@gmail.com.
authors.forename: T S
authors.initials: TS
authors.lastname: Demina
authors.affiliation: Enikolopov Institute of Synthetic Polymer Materials, Russian Academy of Sciences, Profsoyuznaya Str., 70, 117393 Moscow, Russia.
authors.forename: T A
authors.initials: TA
authors.lastname: Akopova
authors.affiliation: Semenov Institute of Chemical Physics, Russian Academy of Sciences, Kosygin Str., 4, 117977 Moscow, Russia.
authors.forename: L V
authors.initials: LV
authors.lastname: Vladimirov
authors.affiliation: Enikolopov Institute of Synthetic Polymer Materials, Russian Academy of Sciences, Profsoyuznaya Str., 70, 117393 Moscow, Russia.
authors.forename: A N
authors.initials: AN
authors.lastname: Zelenetskii
authors.affiliation: Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Str., 16/10, 117997 Moscow, Russia.
authors.forename: E A
authors.initials: EA
authors.lastname: Markvicheva
authors.affiliation: Interfacultary Research Centre on Biomaterials (CEIB), University of Liège, Chemistry Institute, B6C, Allée du 6 août 11, Sart-Tilman, B-4000 Liege, Belgium.
authors.forename: Ch
authors.initials: C
authors.lastname: Grandfils
mesh_terms: D048271:Chitosan / Q000737:chemistry*; D004146:Dioxanes / Q000737:chemistry*; D008863:Microspheres; D011091:Polyesters / Q000138:chemical synthesis / Q000737:chemistry
chemical_list: D004146:Dioxanes; D011091:Polyesters; C033616:poly(lactide); D048271:Chitosan; C091880:dilactide
keywords: Chitosan; Graft copolymers; Microparticles; Oil/water emulsion; Solid-state reactive blending; Tissue engineering; d,l-Lactide