title: Studies on the biosynthesis of ralfuranones in Ralstonia solanacearum.
abstract: Ralfuranones, aryl-furanone secondary metabolites, are involved in the virulence of Ralstonia solanacearum in solanaceous plants. Ralfuranone I (6) has been suggested as a biosynthetic precursor for other ralfuranones; however, this conversion has not been confirmed. We herein investigate the biosynthesis of ralfuranones using feeding experiments with ralfuranone I (6) and its putative metabolite, ralfuranone B (2). The results obtained demonstrated that the biosynthesis of ralfuranones proceeded in enzymatic and non-enzymatic manners.
authors.affiliation: a Graduate School of Life and Environmental Sciences , Osaka Prefecture University , Osaka , Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Kai
authors.affiliation: a Graduate School of Life and Environmental Sciences , Osaka Prefecture University , Osaka , Japan.
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Ohnishi
authors.affiliation: b Laboratory of Plant Pathology and Biotechnology , Kochi University , Kochi , Japan.
authors.forename: Akinori
authors.initials: A
authors.lastname: Kiba
authors.affiliation: c Research Institute of Molecular Genetics , Kochi University , Kochi , Japan.
authors.forename: Kouhei
authors.initials: K
authors.lastname: Ohnishi
authors.affiliation: b Laboratory of Plant Pathology and Biotechnology , Kochi University , Kochi , Japan.
authors.forename: Yasufumi
authors.initials: Y
authors.lastname: Hikichi
mesh_terms: D002851:Chromatography, High Pressure Liquid; D007783:Lactones / Q000378:metabolism*; D066244:Proton Magnetic Resonance Spectroscopy; D043368:Ralstonia solanacearum / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D007783:Lactones; C000602065:ralfuranone A; C000602066:ralfuranone B
keywords: Ralstonia solanacearum; biosynthesis; furanone synthase; quorum sensing; ralfuranones