title: Absolute quantification of 35 plasma biomarkers in human saliva using targeted MS.
abstract: BACKGROUND
  Although the use of human saliva for diagnosing disease has been known to be of great clinical potential, few attempts have been made so far to develop its use. In this work, we developed an MRM-MS approach for 35 plasma biomarkers using human saliva in a clinical environment.
  
  
  RESULTS
  A 30-min micro LC-MS/MS run in MRM mode was conducted in order to quantify the 35 plasma proteins in human saliva. Sample preparation procedures were performed in quadruplicate and analyzed in duplicate. Results show that 32 of the 35 plasma proteins were quantified in human saliva using calibration curves in the 2- log10 dynamic ranges with excellent linearity.
  
  
  CONCLUSIONS
  Our MRM method is compatible with routine measurements in daily clinical practice.
authors.affiliation: Laboratoire de Biochimie et de Protéomique Clinique- Institut de Médecine Régénérative et Biothérapies (LBPC-IRMB), CHU de Montpellier, 80 rue Augustin Fliche, Montpellier, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Hirtz
authors.affiliation: Laboratoire de Biochimie et de Protéomique Clinique- Institut de Médecine Régénérative et Biothérapies (LBPC-IRMB), CHU de Montpellier, 80 rue Augustin Fliche, Montpellier, France.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Vialaret
authors.affiliation: Laboratoire de Biochimie et de Protéomique Clinique- Institut de Médecine Régénérative et Biothérapies (LBPC-IRMB), CHU de Montpellier, 80 rue Augustin Fliche, Montpellier, France.
authors.forename: Nora
authors.initials: N
authors.lastname: Nowak
authors.affiliation: Laboratoire de Biochimie et de Protéomique Clinique- Institut de Médecine Régénérative et Biothérapies (LBPC-IRMB), CHU de Montpellier, 80 rue Augustin Fliche, Montpellier, France.|Centre Mémoire Ressources Recherche Languedoc-Roussillon, CHRU de Montpellier, hôpital Gui de Chauliac, Montpellier, France.|Université de Montpellier, Montpellier, France.
authors.forename: Audrey
authors.initials: A
authors.lastname: Gabelle
authors.affiliation: Laboratoire de Biochimie et de Protéomique Clinique- Institut de Médecine Régénérative et Biothérapies (LBPC-IRMB), CHU de Montpellier, 80 rue Augustin Fliche, Montpellier, France.
authors.forename: Dominique
authors.initials: D
authors.lastname: Deville de Périère
authors.affiliation: Laboratoire de Biochimie et de Protéomique Clinique- Institut de Médecine Régénérative et Biothérapies (LBPC-IRMB), CHU de Montpellier, 80 rue Augustin Fliche, Montpellier, France.
authors.forename: Sylvain
authors.initials: S
authors.lastname: Lehmann
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood; D001798:Blood Proteins / Q000032:analysis; D019963:Clinical Chemistry Tests / Q000379:methods*; D005240:Feasibility Studies; D005260:Female; D006801:Humans; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D012463:Saliva / Q000737:chemistry*; D055815:Young Adult
chemical_list: D015415:Biomarkers; D001798:Blood Proteins
keywords: MS; biochemistry; clinical chemistry; proteomics; saliva