title: A rapid PCR assay to characterize the intact pks15/1 gene, a virulence marker in Mycobacterium tuberculosis.
abstract: Intact pks15/1 is involved in the biosynthesis of phenolic glycolipids and proposed as a marker for virulence and phylogeny in tuberculosis. Identification of intact condition is achieved mainly by DNA sequencing. For this reason the aim of this study was to develop a reproducible endpoint PCR-assay to characterize it.
authors.affiliation: Instituto de Salud Pública, Universidad Veracruzana, Veracruz, Mexico. Electronic address: robzencue@gmail.com.
authors.forename: Roberto
authors.initials: R
authors.lastname: Zenteno-Cuevas
authors.affiliation: Instituto de Salud Pública, Universidad Veracruzana, Veracruz, Mexico.
authors.forename: Rodrigo Javier
authors.initials: RJ
authors.lastname: Hernandez-Morales
authors.affiliation: Dirección de Investigación, Hospital General de México "Dr. Eduardo Liceaga", México, D.F., Mexico.
authors.forename: Lucia Monserrat
authors.initials: LM
authors.lastname: Pérez-Navarro
authors.affiliation: Laboratorio de Epidemiología y Ecología y Molecular, Escuela de Ciencias de la Salud, Universidad Autónoma de Baja California, Baja California, Mexico.
authors.forename: Raquel
authors.initials: R
authors.lastname: Muñiz-Salazar
authors.affiliation: Instituto de Investigaciones Biológicas, Universidad Veracruzana, Veracruz, México.
authors.forename: Juan
authors.initials: J
authors.lastname: Santiago-García
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques / Q000379:methods; D001483:Base Sequence; D015415:Biomarkers / Q000032:analysis; D002876:Chromosomes, Bacterial; D006017:Glycolipids / Q000096:biosynthesis; D009169:Mycobacterium tuberculosis / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity; D010802:Phylogeny; D048630:Polyketide Synthases / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA; D013183:Sputum / Q000382:microbiology; D014376:Tuberculosis / Q000175:diagnosis / Q000382:microbiology; D014774:Virulence / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D015415:Biomarkers; D006017:Glycolipids; D048630:Polyketide Synthases
keywords: PCR-assay; Tuberculosis; pks15/1