title: [Purification, identification and characterization of an endoglucanase Egn20 from Fusarium sp. Q7-31T].
abstract: OBJECTIVE
  The endoglucanase from Fusarium sp. Q7-31T was isolated, purified, identified and characterized to provide data for enzyme system of Fusarium sp. . [Methods] Strain was cultured in liquid fermentation with oat straw as carbon source, the endoglucanase was purified by using Sephacry S-100 chromatography and DEAE-sepharose ion-exchange column chromatography and the enzymatic properties were studied. The protein was identified using MADIL-TOF-TOF.
  
  
  RESULTS
  An endoglucanase was purified and named Egn20. The molecular weight was 55.37 kDa and isoelectric point (pI) was 7.44. Egn20 had optimal activity with carboxymethyl cellulose at 40 degrees C and pH 6.0, stabilized at 45 degrees C and pH 5.0 - 7.0, activated by Fe2+, inhibited by Na+, Ca2+, Mg2+, Zn2+, K+ and inactivated by Hg2+. The enzymatic properties and MADIL-TOF-TOF results suggested that Egn20 belongs to GH7 family.
  
  
  CONCLUSIONS
  Our results may provide important data for the study of Fusarium sp. enzyme system.
authors.forename: Fei
authors.initials: F
authors.lastname: Tian
authors.forename: Zhanling
authors.initials: Z
authors.lastname: Xie
authors.forename: Jing
authors.initials: J
authors.lastname: Guo
authors.forename: Lianzheng
authors.initials: L
authors.lastname: Zhao
authors.forename: Xingbao
authors.initials: X
authors.lastname: Han
authors.forename: Xinyuan
authors.initials: X
authors.lastname: Chang
mesh_terms: D002480:Cellulase / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D004795:Enzyme Stability; D005656:Fungal Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D005670:Fusarium / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics; D005936:Glucans / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008970:Molecular Weight; D013379:Substrate Specificity; D013696:Temperature
chemical_list: D005656:Fungal Proteins; D005936:Glucans; D002480:Cellulase