title: Only a minority of broad-range detoxification genes respond to a variety of phytotoxins in generalist Bemisia tabaci species.
abstract: Generalist insect can utilize two different modes for regulating their detoxification genes, the constitutive mode and the induced mode. Here, we used the Bemisia tabaci sibling species MEAM1 and MED, as a model system for studying constitutive and induced detoxification resistance and their associated tradeoffs. B. tabaci adults were allowed to feed through membranes for 24 h on diet containing only sucrose or sucrose with various phytotoxins. Quantitative real-time PCR analyses of 18 detoxification genes, indicated that relatively few transcripts were changed in both the MEAM1 and MED species, in response to the addition of phytotoxins to the diet. Induced transcription of detoxification genes only in the MED species, in response to the presence of indole-3-carbinol in the insect's diet, was correlated with maintenance of reproductive performance in comparison to significant reduction in performance of the MEAM1 species. Three genes, COE2, CYP6-like 5 and BtGST2, responded to more than one compound and were highly transcribed in the insect gut. Furthermore, functional assays showed that the BtGST2 gene encodes a protein capable of interacting with both flavonoids and glucosinolates. In conclusion, several detoxification genes were identified that could potentially be involved in the adaptation of B. tabaci to its host plants.
authors.affiliation: Department of Entomology, the Hebrew University of Jerusalem, Rehovot 76100, Israel.
authors.forename: Eyal
authors.initials: E
authors.lastname: Halon
authors.affiliation: Department of Entomology, the Hebrew University of Jerusalem, Rehovot 76100, Israel.
authors.forename: Galit
authors.initials: G
authors.lastname: Eakteiman
authors.affiliation: Department of Entomology, the Hebrew University of Jerusalem, Rehovot 76100, Israel.
authors.forename: Pnina
authors.initials: P
authors.lastname: Moshitzky
authors.affiliation: Department of Entomology, the Hebrew University of Jerusalem, Rehovot 76100, Israel.
authors.forename: Moshe
authors.initials: M
authors.lastname: Elbaz
authors.affiliation: Department of Entomology, the Hebrew University of Jerusalem, Rehovot 76100, Israel.
authors.forename: Michal
authors.initials: M
authors.lastname: Alon
authors.affiliation: Department of Biology, University of Crete, Heraklion, Crete 71409, Greece.
authors.forename: Nena
authors.initials: N
authors.lastname: Pavlidi
authors.affiliation: Institute of Molecular Biology &Biotechnology, Foundation for Research &Technology Hellas, Heraklion, Crete, Greece.|Laboratory of Pesticide Science, Department of Crop Science, Agricultural University of Athens, Athens, Greece.
authors.forename: John
authors.initials: J
authors.lastname: Vontas
authors.affiliation: Department of Entomology, the Hebrew University of Jerusalem, Rehovot 76100, Israel.
authors.forename: Shai
authors.initials: S
authors.lastname: Morin
mesh_terms: D000818:Animals; D016000:Cluster Analysis; D004791:Enzyme Inhibitors / Q000494:pharmacology; D041981:Gastrointestinal Tract / Q000378:metabolism; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D017344:Genes, Insect; D005982:Glutathione Transferase / Q000037:antagonists & inhibitors / Q000378:metabolism; D006430:Hemiptera / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism*; D008658:Inactivation, Metabolic / Q000235:genetics*; D007700:Kinetics; D015203:Reproducibility of Results; D013379:Substrate Specificity; D014118:Toxins, Biological / Q000378:metabolism*; D014158:Transcription, Genetic; D015262:Xenobiotics / Q000378:metabolism / Q000494:pharmacology
chemical_list: D004791:Enzyme Inhibitors; D014118:Toxins, Biological; D015262:Xenobiotics; D005982:Glutathione Transferase