title: Lyophilized allografts without pre-treatment with glutaraldehyde are more suitable than cryopreserved allografts for pulmonary artery reconstruction.
abstract: Various methods are available for preservation of vascular grafts for pulmonary artery (PA) replacement. Lyophilization and cryopreservation reduce antigenicity and prevent thrombosis and calcification in vascular grafts, so both methods can be used to obtain vascular bioprostheses. We evaluated the hemodynamic, gasometric, imaging, and macroscopic and microscopic findings produced by PA reconstruction with lyophilized (LyoPA) grafts and cryopreserved (CryoPA) grafts in dogs. Eighteen healthy crossbred adult dogs of both sexes weighing between 18 and 20 kg were used and divided into three groups of six: group I, PA section and reanastomosis; group II, PA resection and reconstruction with LyoPA allograft; group III, PA resection and reconstruction with CryoPA allograft. Dogs were evaluated 4 weeks after surgery, and the status of the graft and vascular anastomosis were examined macroscopically and microscopically. No clinical, radiologic, or blood-gas abnormalities were observed during the study. The mean pulmonary artery pressure (MPAP) in group III increased significantly at the end of the study compared with baseline (P=0.02) and final [P=0.007, two-way repeat-measures analysis of variance (RM ANOVA)] values. Pulmonary vascular resistance of groups II and III increased immediately after reperfusion and also at the end of the study compared to baseline. The increase shown by group III vs group I was significant only if compared with after surgery and study end (P=0.016 and P=0.005, respectively, two-way RM ANOVA). Microscopically, permeability was reduced by ≤75% in group III. In conclusion, substitution of PAs with LyoPA grafts is technically feasible and clinically promising.
authors.affiliation: Department of Experimental Surgery, National Institute of Respiratory Diseases "Ismael Cosío Villegas", Mexico City, Mexico.
authors.forename: J R
authors.initials: JR
authors.lastname: Olmos-Zúñiga
authors.affiliation: Department of Experimental Surgery, National Institute of Respiratory Diseases "Ismael Cosío Villegas", Mexico City, Mexico.
authors.forename: R
authors.initials: R
authors.lastname: Jasso-Victoria
authors.affiliation: Medical and Pharmaceutical Biotechnology, Center for Research and Assistance in Technology and Design of the State of Jalisco, Guadalajara, Jalisco, Mexico.
authors.forename: N E
authors.initials: NE
authors.lastname: Díaz-Martínez
authors.affiliation: Laboratory of Morphology, National Institute of Respiratory Diseases "Ismael Cosío Villegas", Mexico City, Mexico.
authors.forename: M O
authors.initials: MO
authors.lastname: Gaxiola-Gaxiola
authors.affiliation: Department of Experimental Surgery, National Institute of Respiratory Diseases "Ismael Cosío Villegas", Mexico City, Mexico.
authors.forename: A
authors.initials: A
authors.lastname: Sotres-Vega
authors.affiliation: Department of Experimental Surgery, National Institute of Respiratory Diseases "Ismael Cosío Villegas", Mexico City, Mexico.
authors.forename: Y
authors.initials: Y
authors.lastname: Heras-Romero
authors.affiliation: Department of Experimental Surgery, National Institute of Respiratory Diseases "Ismael Cosío Villegas", Mexico City, Mexico.
authors.forename: M
authors.initials: M
authors.lastname: Baltazares-Lipp
authors.affiliation: Hemodynamics and Echocardiography Service, National Institute of Respiratory Diseases "Ismael Cosío Villegas", Mexico City, Mexico.
authors.forename: M E
authors.initials: ME
authors.lastname: Baltazares-Lipp
authors.affiliation: Medical Administration, National Institute of Respiratory Diseases "Ismael Cosío Villegas", Mexico City, Mexico.
authors.forename: P
authors.initials: P
authors.lastname: Santillán-Doherty
authors.affiliation: Department of Experimental Surgery, National Institute of Respiratory Diseases "Ismael Cosío Villegas", Mexico City, Mexico.
authors.forename: C
authors.initials: C
authors.lastname: Hernández-Jiménez
mesh_terms: D064591:Allografts / Q000033:anatomy & histology / Q000502:physiology* / Q000601:surgery; D000704:Analysis of Variance; D000818:Animals; D001794:Blood Pressure; D001807:Blood Vessel Prosthesis / Q000009:adverse effects; D019917:Blood Vessel Prosthesis Implantation / Q000379:methods*; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents; D004285:Dogs; D005260:Female; D005612:Freeze Drying / Q000379:methods*; D005976:Glutaral; D008297:Male; D011651:Pulmonary Artery / Q000473:pathology / Q000502:physiology; D011652:Pulmonary Circulation; D014184:Transplantation, Homologous; D014655:Vascular Resistance
chemical_list: D003451:Cryoprotective Agents; D005976:Glutaral