title: Amplified binding-induced homogeneous assay through catalytic cycling of analyte for ultrasensitive protein detection.
abstract: By using the principle of binding-induced DNA assembly, we have developed a novel homogeneous assay that is able to convert an affinity protein binding event into a predesigned DNA assembly. The assembled DNA sequence can be ligated into an intact DNA strand and hundreds of DNA hairpins can be cleaved by a nicking endonuclease. Each cleavage releases a single-stranded DNA (ssDNA) probe that is initially caged in the DNA hairpin. This released ssDNA probe can then turn on the fluorescence signal by desorbing a fluorescently-labelled complementary DNA probe from graphene oxide through hybridization. We demonstrate that this homogeneous, isothermal, and amplifiable assay can be tailored to detect a number of proteins, including a cancer biomarker, human prostate specific antigen, at picomolar levels in both buffer and human serum samples.
authors.affiliation: Analytical & Testing Center, Sichuan University, 29 Wangjiang Road, Chengdu 610064, China. houxd@scu.edu.cn and Department of Laboratory Medicine and Pathology, Faculty of Medicine and Dentistry, University of Alberta, 10-102 Clinical Sciences Building, Edmonton, Alberta T6G 2G3, Canada. hongquan@ualberta.ca.
authors.forename: Junbo
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Laboratory Medicine and Pathology, Faculty of Medicine and Dentistry, University of Alberta, 10-102 Clinical Sciences Building, Edmonton, Alberta T6G 2G3, Canada. hongquan@ualberta.ca.
authors.forename: Bin
authors.initials: B
authors.lastname: Deng
authors.affiliation: Analytical & Testing Center, Sichuan University, 29 Wangjiang Road, Chengdu 610064, China. houxd@scu.edu.cn.
authors.forename: Peng
authors.initials: P
authors.lastname: Wu
authors.affiliation: Department of Laboratory Medicine and Pathology, Faculty of Medicine and Dentistry, University of Alberta, 10-102 Clinical Sciences Building, Edmonton, Alberta T6G 2G3, Canada. hongquan@ualberta.ca.
authors.forename: Feng
authors.initials: F
authors.lastname: Li
authors.affiliation: Department of Laboratory Medicine and Pathology, Faculty of Medicine and Dentistry, University of Alberta, 10-102 Clinical Sciences Building, Edmonton, Alberta T6G 2G3, Canada. hongquan@ualberta.ca.
authors.forename: Xing-Fang
authors.initials: XF
authors.lastname: Li
authors.affiliation: Department of Laboratory Medicine and Pathology, Faculty of Medicine and Dentistry, University of Alberta, 10-102 Clinical Sciences Building, Edmonton, Alberta T6G 2G3, Canada. hongquan@ualberta.ca.
authors.forename: X Chris
authors.initials: XC
authors.lastname: Le
authors.affiliation: Department of Laboratory Medicine and Pathology, Faculty of Medicine and Dentistry, University of Alberta, 10-102 Clinical Sciences Building, Edmonton, Alberta T6G 2G3, Canada. hongquan@ualberta.ca.
authors.forename: Hongquan
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Analytical & Testing Center, Sichuan University, 29 Wangjiang Road, Chengdu 610064, China. houxd@scu.edu.cn and College of Chemistry, Sichuan University, 29 Wangjiang Road, Chengdu 610064, China.
authors.forename: Xiandeng
authors.initials: X
authors.lastname: Hou
mesh_terms: D002384:Catalysis; D057230:Limit of Detection; D011506:Proteins / Q000032:analysis*
chemical_list: D011506:Proteins