title: Extraction Optimization, Characterization and Bioactivities of a Major Polysaccharide from Sargassum thunbergii.
abstract: Sargassum thunbergii is a kind of natural edible algae. STP (S. thunbergii polysaccharides) was considered as the main bioactive compounds in S. thunbergii. To obtain the optimal processing conditions for maximum total sugar yield, single factor investigation and response surface methodology (RSM) were employed. The optimal processing conditions were as follows: liquid to solid ratio 120 mL/g, extraction time 210 min, extraction temperature 97°C. The experimental yield 7.53% under optimized conditions was closely agreed with the predicted yield 7.85% of the model. The major polysaccharide fraction from S. thunbergii (named STP-II) was purified by DEAE-Sepharose CL-6B column chromatography. High-performance size-exclusion chromatography (HPSEC), gas chromatography (GC) and high-performance liquid chromatography (HPLC) were used to identify its characterizations, and in vitro antioxidant assays and cytotoxicity assays were used to research its bioactivities. The purified fraction STP-II (63.75%) was a single peak in HPSEC with Sugar KS-804 column, had a molecular weight of 550KD, and comprised mainly of fucose, xylose, galactose, glucose and glucuronic acid. STP-II had higher scavenging activities on hydroxyl radical (76.72% at 0.7 mg/mL) and superoxide radical (95.17% at 2 mg/mL) than Vitamin C (Vc). STP-II also exhibited the capability of anti-proliferation in Caco-2 cells. STP-II possessed good antioxidant and inhibitory activity against human colon cancer Caco-2 cells in vitro and could be explored as novel natural functional food.
authors.affiliation: Department of Bioengineering and Biotechnology, Huaqiao University, Fujian Xiamen, China.
authors.forename: Xiumei
authors.initials: X
authors.lastname: Yuan
authors.affiliation: Department of Bioengineering and Biotechnology, Huaqiao University, Fujian Xiamen, China.
authors.forename: Yawei
authors.initials: Y
authors.lastname: Zeng
authors.affiliation: Department of Bioengineering and Biotechnology, Huaqiao University, Fujian Xiamen, China.
authors.forename: Kaiying
authors.initials: K
authors.lastname: Nie
authors.affiliation: Department of Bioengineering and Biotechnology, Huaqiao University, Fujian Xiamen, China.
authors.forename: Dianhui
authors.initials: D
authors.lastname: Luo
authors.affiliation: Department of Bioengineering and Biotechnology, Huaqiao University, Fujian Xiamen, China.
authors.forename: Zhaojing
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D018938:Caco-2 Cells; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D055951:Functional Food; D006801:Humans; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D044647:Sargassum / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D011134:Polysaccharides