title: Cell adhesion on NiTi thin film sputter-deposited meshes.
abstract: Scaffolds for tissue engineering enable the possibility to fabricate and form biomedical implants in vitro, which fulfill special functionality in vivo. In this study, free-standing Nickel–Titanium(NiTi) thin film mesheswere produced by means of magnetron sputter deposition.Meshes contained precisely defined rhombic holes in the size of 440 to 1309 μm2 and a strut width ranging from 5.3 to 9.2 μm. The effective mechanical properties of the microstructured superelastic NiTi thin film were examined by tensile testing. These results will be adapted for the design of the holes in the film. The influence of hole and strut dimensions on the adhesion of sheep autologous cells (CD133+) was studied after 24 h and after seven days of incubation. Optical analysis using fluorescence microscopy and scanning electron microscopy showed that cell adhesion depends on the structural parameters of the mesh. After 7 days in cell culture a large part of the mesh was covered with aligned fibrous material. Cell adhesion is particularly facilitated on meshes with small rhombic holes of 440 μm2 and a strut width of 5.3 μm. Our results demonstrate that free-standing NiTi thin film meshes have a promising potential for applicationsin cardiovascular tissue engineering, particularly for the fabrication of heart valves.
authors.affiliation: Inorganic Functional Materials, Institute for Materials Science, Faculty of Engineering, University of Kiel, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Loger
authors.affiliation: Department of Cardiovascular Surgery, University Hospital of Schleswig-Holstein, Kiel, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Engel
authors.affiliation: Department of Cardiovascular Surgery, University Hospital of Schleswig-Holstein, Kiel, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Haupt
authors.affiliation: Biocompatible Nanomaterials, Institute for Materials Science, Faculty of Engineering, University of Kiel, Germany.
authors.forename: Q
authors.initials: Q
authors.lastname: Li
authors.affiliation: Inorganic Functional Materials, Institute for Materials Science, Faculty of Engineering, University of Kiel, Germany; ACQUANDAS GmbH, Kiel, Germany.
authors.forename: R
authors.initials: R
authors.lastname: Lima de Miranda
authors.affiliation: Inorganic Functional Materials, Institute for Materials Science, Faculty of Engineering, University of Kiel, Germany.
authors.forename: E
authors.initials: E
authors.lastname: Quandt
authors.affiliation: Department of Cardiovascular Surgery, University Hospital of Schleswig-Holstein, Kiel, Germany.
authors.forename: G
authors.initials: G
authors.lastname: Lutter
authors.affiliation: Biocompatible Nanomaterials, Institute for Materials Science, Faculty of Engineering, University of Kiel, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Selhuber-Unkel
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects*; D002478:Cells, Cultured; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects; D009532:Nickel / Q000737:chemistry / Q000494:pharmacology*; D012756:Sheep; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*; D014025:Titanium / Q000737:chemistry / Q000494:pharmacology*
chemical_list: C040654:titanium nickelide; D009532:Nickel; D014025:Titanium
keywords: Cell adhesion; Mechanical properties; NiTi; Shape memory alloy; Sputter deposition; Thin film scaffold