title: Pseudorhodobacter psychrotoleranssp. nov., a psychrotolerant bacterium isolated from terrestrial soil, and emended description of the genusPseudorhodobacter.
abstract: A Gram-stain-negative, facultatively aerobic, cream-coloured, ovoid-shaped, non-motile and psychrotolerant bacterial strain, PAMC 27389T, was isolated from terrestrial soil collected on King George Island, Antarctica. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain PAMC 27389Tbelongs to the genusPseudorhodobacter, sharing highest similarities with the type strains ofPseudorhodobacter wandonensis(96.9 %),Pseudorhodobacter antarcticus(96.8 %),Pseudorhodobacter ferrugineus(96.5 %) andPseudorhodobacter aquimaris(95.4 %). Average nucleotide identity values between strain PAMC 27389Tand the type strains ofP. wandonensis,P. antarcticus,P. ferrugineusandP. aquimariswere 70.8, 70.9, 71.0 and 70.5 %, respectively and the genome-to-genome distances were 18.4-19.1 %, indicating PAMC 27389Tis clearly distinguished from the most closely relatedPseudorhodobacterspecies. The genomic DNA G+C content was 60.1 mol%. Strain PAMC 27389Tgrew at 0-37 °C (optimally at 15-20 °C), at pH 5.5-9.0 (optimally at pH 6.5-7.0) and in the presence of 0.5-3.0 % (w/v) sea salt (optimally with 0.5 %). It lacked bacteriochlorophylla. The major fatty acids (>5 %) were summed feature 8 (C18 : 1ω7cand/or C18 : 1ω6c) and C18 : 1ω7c11-methyl. The major polar lipids were phosphatidylcholine, phosphatidylglycerol, an unidentified phospholipid, an unidentified aminolipid, an unidentified lipid and three unidentified aminophospholipids. The major respiratory quinone was Q-10. Based on the phenotypic, chemotaxonomic and genomic data presented, we propose the namePseudorhodobacter psychrotoleranssp. nov. with the type strain PAMC 27389T( = KCTC 42640T= JCM 30764T).
authors.affiliation: Division of Polar Life Sciences, Korea Polar Research Institute, 26 Songdomirae-ro, Yeonsu-gu, Incheon 406-840, Republic of Korea.|School of Biological Sciences, College of Natural Science, Seoul National University, 599 Gwanak-ro, Gwanak-gu, Seoul 151-747, Republic of Korea.
authors.forename: Y M
authors.initials: YM
authors.lastname: Lee
authors.affiliation: Division of Polar Life Sciences, Korea Polar Research Institute, 26 Songdomirae-ro, Yeonsu-gu, Incheon 406-840, Republic of Korea.
authors.forename: J Y
authors.initials: JY
authors.lastname: Yang
authors.affiliation: Division of Polar Life Sciences, Korea Polar Research Institute, 26 Songdomirae-ro, Yeonsu-gu, Incheon 406-840, Republic of Korea.
authors.forename: K
authors.initials: K
authors.lastname: Baek
authors.affiliation: Division of Polar Life Sciences, Korea Polar Research Institute, 26 Songdomirae-ro, Yeonsu-gu, Incheon 406-840, Republic of Korea.
authors.forename: S J
authors.initials: SJ
authors.lastname: Han
authors.affiliation: Division of Polar Life Sciences, Korea Polar Research Institute, 26 Songdomirae-ro, Yeonsu-gu, Incheon 406-840, Republic of Korea.
authors.forename: S C
authors.initials: SC
authors.lastname: Shin
authors.affiliation: Division of Polar Life Sciences, Korea Polar Research Institute, 26 Songdomirae-ro, Yeonsu-gu, Incheon 406-840, Republic of Korea.
authors.forename: C Y
authors.initials: CY
authors.lastname: Hwang
authors.affiliation: Division of Polar Life Sciences, Korea Polar Research Institute, 26 Songdomirae-ro, Yeonsu-gu, Incheon 406-840, Republic of Korea.
authors.forename: S G
authors.initials: SG
authors.lastname: Hong
authors.affiliation: Division of Polar Life Sciences, Korea Polar Research Institute, 26 Songdomirae-ro, Yeonsu-gu, Incheon 406-840, Republic of Korea.
authors.forename: H K
authors.initials: HK
authors.lastname: Lee