title: Osmotic membrane bioreactor for phenol biodegradation under continuous operation.
abstract: Continuous phenol biodegradation was accomplished in a two-phase partitioning osmotic membrane bioreactor (TPPOMBR) system, using extractant impregnated membranes (EIM) as the partitioning phase. The EIMs alleviated substrate inhibition during prolonged operation at influent phenol concentrations of 600-2000mg/L, and also at spiked concentrations of 2500mg/L phenol restricted to 2 days. Filtration of the effluent through forward osmosis maintained high biomass concentration in the bioreactor and improved effluent quality. Steady state was reached in 5-6 days at removal rates varying between 2000 and 5500mg/L-day under various conditions. Due to biofouling and salt accumulation, the permeate flux varied from 1.2-7.2 LMH during 54 days of operation, while maintaining an average hydraulic retention time of 7.4h. A washing cycle, comprising 1h osmotic backwashing using 0.5M NaCl and 2h washing with water, facilitated biofilm removal from the membranes. Characterization of the extracellular polymeric substances (EPS) through FTIR showed peaks between 1700 and 1500cm(-1), 1450-1450cm(-1) and 1200-1000cm(-1), indicating the presence of proteins, phenols and polysaccharides, respectively. The carbohydrate to protein ratio in the EPS was estimated to be 0.3. These results indicate that TPPOMBR can be promising in continuous treatment of phenolic wastewater.
authors.affiliation: Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, 117585, Singapore.
authors.forename: Prashant
authors.initials: P
authors.lastname: Praveen
authors.affiliation: Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, 117585, Singapore. Electronic address: chelohkc@nus.edu.sg.
authors.forename: Kai-Chee
authors.initials: KC
authors.lastname: Loh
mesh_terms: D019149:Bioreactors; D008567:Membranes, Artificial; D009995:Osmosis; D019800:Phenol / Q000378:metabolism*; D016958:Pseudomonas putida / Q000378:metabolism*; D014865:Waste Disposal, Fluid / Q000379:methods; D014874:Water Pollutants, Chemical / Q000378:metabolism*; D018508:Water Purification / Q000379:methods
chemical_list: D008567:Membranes, Artificial; D014874:Water Pollutants, Chemical; D019800:Phenol
keywords: Biodegradation; Forward osmosis; Membrane bioreactor; Phenol; Two-phase partitioning bioreactor