title: The effects of nickel(II) complexes with imidazole derivatives on pyocyanin and pyoverdine production by Pseudomonas aeruginosa strains isolated from cystic fibrosis.
abstract: Pseudomonas aeruginosa infection is problematic in patients with cystic fibrosis (CF). P. aeruginosa secretes a diversity of pigments, such as pyocyanin and pyoverdine. The aim of this study was to evaluate the effects of complexes of nickel(II) ([Ni(iaa)2(H2O)2]·H2O (iaa = imidazole-4-acetate anion), [Ni(1-allim)6](NO3)2 (1-allim = 1-allylimidazole) and NiCl2 on pyocyanin and pyoverdine production by 23 strains of P. aeruginosa isolated from cystic fibrosis under growth conditions specific for the CF respiratory system. The antibacterial effects and biophysical properties of the tested substances were measured by spectrofluorometric techniques, as well as by laser interferometry, confocal and atomic force microscopy. The cytotoxic properties of all compounds were measured by Annexin/IP assay against A549 cells. All tested compounds have no effect on pyocyanin production and decrease the pyoverdine secretion in about 40% of tested P. aeruginosa strains at non-cytotoxic range of concentrations. Imidazole-4-acetate anion and 1-allylimidazole have good diffusion properties in the mature P. aeruginosa PAO1 biofilm. In conclusion, the tested nickel(II) complexes do not have clinical implications in P. aeruginosa eradication in cystic fibrosis. The diffusion properties of 1-allylimidazole and imidazole-4-acetate and their lack of effect on A549 cells suggest that they might be considered for chemical synthesis with other transition metals.
authors.affiliation: Department of Microbiology, Institute of Biology, Jan Kochanowski University, Kielce, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Gałczyńska
authors.affiliation: Institute of Chemistry, Jan Kochanowski University, Kielce, Poland.
authors.forename: Krystyna
authors.initials: K
authors.lastname: Kurdziel
authors.affiliation: Department of Environment Protection and Modelling, Jan Kochanowski University, Kielce, Poland.
authors.forename: Wioletta
authors.initials: W
authors.lastname: Adamus-Białek
authors.affiliation: Institute of Physics, Jan Kochanowski University in Kielce, Kielce, Poland.
authors.forename: Sławomir
authors.initials: S
authors.lastname: Wąsik
authors.affiliation: Institute of Physics, Jan Kochanowski University in Kielce, Kielce, Poland.
authors.forename: Karol
authors.initials: K
authors.lastname: Szary
authors.affiliation: Institute of Physics, Jan Kochanowski University in Kielce, Kielce, Poland.
authors.forename: Marcin
authors.initials: M
authors.lastname: Drabik
authors.affiliation: Department of Radiobiology and Immunology, Jan Kochanowski University, Kielce, Poland.
authors.forename: Aneta
authors.initials: A
authors.lastname: Węgierek-Ciuk
authors.affiliation: Department of Radiobiology and Immunology, Jan Kochanowski University, Kielce, Poland and Centre for Radiobiology and Biological Dosimetry, Institute for Nuclear Chemistry and Technology, Warsaw, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Lankoff
authors.affiliation: Department of Microbiology, Institute of Biology, Jan Kochanowski University, Kielce, Poland.
authors.forename: Michał
authors.initials: M
authors.lastname: Arabski
mesh_terms: D002460:Cell Line; D003550:Cystic Fibrosis / Q000382:microbiology*; D006801:Humans; D007093:Imidazoles / Q000737:chemistry / Q000494:pharmacology*; D018625:Microscopy, Atomic Force; D009532:Nickel / Q000737:chemistry / Q000494:pharmacology*; D009842:Oligopeptides / Q000096:biosynthesis*; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000302:isolation & purification / Q000378:metabolism; D011710:Pyocyanine / Q000096:biosynthesis*; D013050:Spectrometry, Fluorescence
chemical_list: D007093:Imidazoles; D009842:Oligopeptides; D009532:Nickel; C042453:pyoverdin; D011710:Pyocyanine