title: Interspecific competition and protistan grazing affect the coexistence of freshwater betaproteobacterial strains.
abstract: The competitive strength of four cosmopolitan freshwater betaproteobacterial isolates was investigated in the presence or absence of bacterivorous flagellates during continuous cultivation in artificial minimal medium at two dilution rates. Bacteria reached similar abundance and growth rate in monocultures, but in co-cultures, two strains (Acidovorax sp. and Massilia sp.) displayed significantly higher numbers and growth rates. These potential cross-feeding benefits were also supported by a high nutritional versatility of the two strains. In contrast, Hydrogenophaga sp. was seemingly less competitive or even inhibited by co-cultivation, and Limnohabitans planktonicus displayed striking abundance fluctuations. The latter two strains were least versatile in the uptake of different carbon sources and thus suffered more from interspecific competition. Moreover, remarkable strain-specific responses appeared when bacteria experienced increasing loss rates due to grazing and/or raised dilution rates. Limnohabitans planktonicus developed no successful defence strategy and was close to extinction. Massilia sp. formed grazing-resistant filaments exclusively at low dilution, but was highly reduced at increased flow-through. Acidovorax sp. was selectively ingested, but compensated grazing losses with accelerated growth rates and formed (co-)aggregates together with Hydrogenophaga sp. to escape predation at high flow-through. These species-specific interactions, growth responses and defence strategies strongly modulate mixed microbial assemblages and the microbial food web.
authors.affiliation: Biology Centre CAS, Institute of Hydrobiology, České Budějovice, Czech Republic Limnological Station, University of Zurich, Kilchberg, Switzerland michaelasalcher@gmail.com.
authors.forename: Michaela M
authors.initials: MM
authors.lastname: Salcher
authors.affiliation: Limnological Station, University of Zurich, Kilchberg, Switzerland.
authors.forename: Claudia
authors.initials: C
authors.lastname: Ewert
authors.affiliation: Biology Centre CAS, Institute of Hydrobiology, České Budějovice, Czech Republic.
authors.forename: Karel
authors.initials: K
authors.lastname: Šimek
authors.affiliation: Biology Centre CAS, Institute of Hydrobiology, České Budějovice, Czech Republic.
authors.forename: Vojtěch
authors.initials: V
authors.lastname: Kasalický
authors.affiliation: Limnological Station, University of Zurich, Kilchberg, Switzerland.
authors.forename: Thomas
authors.initials: T
authors.lastname: Posch
mesh_terms: D018920:Coculture Techniques; D042621:Comamonadaceae / Q000254:growth & development* / Q000302:isolation & purification; D020387:Food Chain; D005618:Fresh Water / Q000382:microbiology; D042802:Oxalobacteraceae / Q000254:growth & development* / Q000302:isolation & purification
keywords: Betaproteobacteria; chemostat; co-cultivation; flagellate-selective bacterivory; interspecific competition; synergistic cooperation