title: Fat harvesting site is an important determinant of proliferation and pluripotency of adipose-derived stem cells.
abstract: To define the optimal fat harvest site and detect any potential differences in adipose-derived stem cells (ASCs) proliferation properties in camels, aspirates from the abdomen and hump sites were compared. Obtained results revealed that ASCs from both abdomen and hump exhibited spindle-shaped and fibroblast-like morphology with hump-derived ASCs being smaller in size and narrower in overall appearance than abdominal ASCs. Abdominal ASCs required a greater time for proliferation than the hump-derived cells. These results were further confirmed with a tetrazolium-based colorimetric assay (MTT) which showed a greater cell proliferation rate for hump ASCs than for the abdomen. Under inductive conditions, ASCs from both abdominal and hump fat deposits maintained their lineage differentiation potential into adipogenic, chondrogenic, and osteogenic lineages during subsequent passages without any qualitative difference. However, expression of alkaline phosphatase was higher in osteogenic differentiated cells from the hump compared with those of the abdomen. Moreover, the increase in calcium content in hump-derived stem cells was higher than that in abdominal-derived stem cells. In conclusion, our findings revealed that ASCs can be obtained from different anatomical locations, although ASCs from the hump fat region may be the ideal stem cell sources for use in cell-based therapies.
authors.affiliation: Stem Cell Biology Department, Stem Cell Technology Research Center, Tehran, Iran.
authors.forename: Abdolreza
authors.initials: A
authors.lastname: Ardeshirylajimi
authors.affiliation: Department of Agricultural Biotechnology, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran.
authors.forename: Farjad
authors.initials: F
authors.lastname: Rafeie
authors.affiliation: Islamic Azad University of Varamin, Varamin, Tehran, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Zandi-Karimi
authors.affiliation: Islamic Azad University of Varamin, Varamin, Tehran, Iran.
authors.forename: Ghobad Asgari
authors.initials: GA
authors.lastname: Jaffarabadi
authors.affiliation: Department of Animal and Poultry Science, College of Aburaihan, University of Tehran, Pakdasht, Tehran, Iran. Electronic address: amohammadis@ut.ac.ir.
authors.forename: Abdollah
authors.initials: A
authors.lastname: Mohammadi-Sangcheshmeh
authors.affiliation: Jahad-Agriculture Organization of Golestan Province, Gorgan, Iran.
authors.forename: Rahmat
authors.initials: R
authors.lastname: Samiei
authors.affiliation: Department of Animal Science, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran.
authors.forename: Abdolhakim
authors.initials: A
authors.lastname: Toghdory
authors.affiliation: Department of Biotechnology, College of Science, University of Tehran, Tehran, Iran.
authors.forename: Ehsan
authors.initials: E
authors.lastname: Seyedjafari
authors.affiliation: Department of Immunology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Seyed Mahmoud
authors.initials: SM
authors.lastname: Hashemi
authors.affiliation: Department of Animal Science, Faculty of Agriculture, Erciyes University, Kayseri, Turkey.
authors.forename: Mehmet Ulas
authors.initials: MU
authors.lastname: Cinar
authors.affiliation: Department of Animal Science, Food and Nutrition, Southern Illinois University, Carbondale, IL, USA.
authors.forename: Eduardo L
authors.initials: EL
authors.lastname: Gastal
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D002162:Camelus; D049109:Cell Proliferation; D002469:Cell Separation; D002478:Cells, Cultured; D039904:Pluripotent Stem Cells / Q000166:cytology*
keywords: Adipogenic; Adipose-derived stem cells; Camel; Chondrogenic; Harvesting site; Osteogenic