title: Stochastic Micro-Pattern for Automated Correlative Fluorescence - Scanning Electron Microscopy.
abstract: Studies of cellular surface features gain from correlative approaches, where live cell information acquired by fluorescence light microscopy is complemented by ultrastructural information from scanning electron micrographs. Current approaches to spatially align fluorescence images with scanning electron micrographs are technically challenging and often cost or time-intensive. Relying exclusively on open-source software and equipment available in a standard lab, we have developed a method for rapid, software-assisted alignment of fluorescence images with the corresponding scanning electron micrographs via a stochastic gold micro-pattern. Here, we provide detailed instructions for micro-pattern production and image processing, troubleshooting for critical intermediate steps, and examples of membrane ultra-structures aligned with the fluorescence signal of proteins enriched at such sites. Together, the presented method for correlative fluorescence - scanning electron microscopy is versatile, robust and easily integrated into existing workflows, permitting image alignment with accuracy comparable to existing approaches with negligible investment of time or capital.
authors.affiliation: DFG Cluster of Excellence 'Cells in Motion', (EXC 1003).|Institute of Medical Physics and Biophysics, University of Münster, Germany.
authors.forename: Isabell
authors.initials: I
authors.lastname: Begemann
authors.affiliation: DFG Cluster of Excellence 'Cells in Motion', (EXC 1003).|Institute of Medical Physics and Biophysics, University of Münster, Germany.
authors.forename: Abhiyan
authors.initials: A
authors.lastname: Viplav
authors.affiliation: Institute of Medical Physics and Biophysics, University of Münster, Germany.
authors.forename: Christiane
authors.initials: C
authors.lastname: Rasch
authors.affiliation: DFG Cluster of Excellence 'Cells in Motion', (EXC 1003).|Institute of Medical Physics and Biophysics, University of Münster, Germany.
authors.forename: Milos
authors.initials: M
authors.lastname: Galic