title: A white spot syndrome virus microRNA promotes the virus infection by targeting the host STAT.
abstract: JAK/STAT pathway plays an important role in invertebrates during virus infection. However the microRNA (miRNA)-mediated regulation of JAK/STAT is not intensively investigated. Viral miRNAs, encoded by virus genome, have emerged as important regulators in the virus-host interactions. In this study, a WSSV (white spot syndrome virus)-encoded miRNA (WSSV-miR-22) was characterized in shrimp during virus infection. The results showed that the viral miRNA could promote WSSV infection in shrimp by targeting the host STAT gene. When the expression of JAK or STAT was knocked down by sequence-specific siRNA, the WSSV copies in shrimp were significantly increased, indicating that the JAK/STAT played positive roles in the antiviral immunity of shrimp. The further findings revealed that TEP1 and TEP2 were the effectors of JAK-STAT signaling pathway. The silencing of TEP1 or TEP2 led to an increase of WSSV copies in shrimp, showing TEP1 and TEP2 were involved in the shrimp immune response against virus infection. Therefore our study presented a novel viral miRNA-mediated JAK/STAT-TEP1/TEP2 signaling pathway in virus infection.
authors.affiliation: Key Laboratory of Animal Virology of Ministry of Agriculture and College of Life Sciences, Zhejiang University, Hangzhou 310058, People's Republic of China.|Jiangsu Key Laboratory for Biodiversity &Biotechnology and Jiangsu Key Laboratory for Aquatic Crustacean Diseases, College of Life Sciences, Nanjing Normal University, Nanjing 210046, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Ren
authors.affiliation: Jiangsu Key Laboratory for Biodiversity &Biotechnology and Jiangsu Key Laboratory for Aquatic Crustacean Diseases, College of Life Sciences, Nanjing Normal University, Nanjing 210046, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Key Laboratory of Animal Virology of Ministry of Agriculture and College of Life Sciences, Zhejiang University, Hangzhou 310058, People's Republic of China.
authors.forename: Yaodong
authors.initials: Y
authors.lastname: He
authors.affiliation: Jiangsu Key Laboratory for Biodiversity &Biotechnology and Jiangsu Key Laboratory for Aquatic Crustacean Diseases, College of Life Sciences, Nanjing Normal University, Nanjing 210046, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Wang
authors.affiliation: Key Laboratory of Animal Virology of Ministry of Agriculture and College of Life Sciences, Zhejiang University, Hangzhou 310058, People's Republic of China.
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000818:Animals; D060829:Arthropod Proteins / Q000378:metabolism*; D035683:MicroRNAs / Q000378:metabolism*; D033561:Penaeidae / Q000378:metabolism* / Q000821:virology; D012367:RNA, Viral / Q000378:metabolism*; D050791:STAT Transcription Factors / Q000378:metabolism*; D015398:Signal Transduction; D046848:White spot syndrome virus 1 / Q000378:metabolism*
chemical_list: D060829:Arthropod Proteins; D035683:MicroRNAs; D012367:RNA, Viral; D050791:STAT Transcription Factors