title: Acute exposure of mercury chloride stimulates the tissue regeneration program and reactive oxygen species production in the Drosophila midgut.
abstract: We used Drosophila as an animal model to study the digestive tract in response to the exposure of inorganic mercury (HgCl2). We found that after oral administration, mercury was mainly sequestered within the midgut. This resulted in increased cell death, which in turn stimulated the tissue regeneration program, including accelerated proliferation and differentiation of the intestinal stem cells (ISCs). We further demonstrated that these injuries correlate closely with the excessive production of the reactive oxygen species (ROS), as vitamin E, an antioxidant reagent, efficiently suppressed the HgCl2-induced phenotypes of midgut and improved the viability. We propose that the Drosophila midgut could serve as a suitable model to study the treatment of acute hydrargyrism on the digestive systems.
authors.affiliation: Key Laboratory of Animal Biotechnology, The Ministry of Agriculture, College of Veterinary Medicine, Northwest A&F University, Yangling 712100, Shaanxi, China. Electronic address: xiaolongzaishi@sina.com.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Key Laboratory of Animal Biotechnology, The Ministry of Agriculture, College of Veterinary Medicine, Northwest A&F University, Yangling 712100, Shaanxi, China.
authors.forename: Xiaochun
authors.initials: X
authors.lastname: Wu
authors.affiliation: Key Laboratory of Animal Biotechnology, The Ministry of Agriculture, College of Veterinary Medicine, Northwest A&F University, Yangling 712100, Shaanxi, China.
authors.forename: Hongjie
authors.initials: H
authors.lastname: Luo
authors.affiliation: Key Laboratory of Animal Biotechnology, The Ministry of Agriculture, College of Veterinary Medicine, Northwest A&F University, Yangling 712100, Shaanxi, China.
authors.forename: Lingling
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Animal Biotechnology, The Ministry of Agriculture, College of Veterinary Medicine, Northwest A&F University, Yangling 712100, Shaanxi, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Ji
authors.affiliation: Key Laboratory of Animal Biotechnology, The Ministry of Agriculture, College of Veterinary Medicine, Northwest A&F University, Yangling 712100, Shaanxi, China.
authors.forename: Xianfeng
authors.initials: X
authors.lastname: Qiao
authors.affiliation: Key Laboratory of Animal Biotechnology, The Ministry of Agriculture, College of Veterinary Medicine, Northwest A&F University, Yangling 712100, Shaanxi, China.
authors.forename: Yaping
authors.initials: Y
authors.lastname: Jin
authors.affiliation: Key Laboratory of Animal Biotechnology, The Ministry of Agriculture, College of Veterinary Medicine, Northwest A&F University, Yangling 712100, Shaanxi, China. Electronic address: wliucn150@yahoo.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002454:Cell Differentiation; D049109:Cell Proliferation; D002470:Cell Survival / Q000187:drug effects; D004064:Digestive System / Q000166:cytology* / Q000187:drug effects; D004330:Drosophila / Q000033:anatomy & histology* / Q000187:drug effects / Q000378:metabolism; D005260:Female; D008297:Male; D008627:Mercuric Chloride / Q000008:administration & dosage* / Q000633:toxicity*; D023421:Models, Animal; D017382:Reactive Oxygen Species / Q000378:metabolism*; D014810:Vitamin E / Q000378:metabolism
chemical_list: D017382:Reactive Oxygen Species; D014810:Vitamin E; D008627:Mercuric Chloride
keywords: Drosophila; Mercury chloride; Midgut; ROS; Vitamin E