title: Nutrient control for stationary phase cellulase production in Trichoderma reesei Rut C-30.
abstract: This work describes the use of nutrient limitations with Trichoderma reesei Rut C-30 to obtain a prolonged stationary phase cellulase production. This period of non-growth may allow for dependable cellulase production, extended fermentation periods, and the possibility to use pellet morphology for easy product separation. Phosphorus limitation was successful in halting growth and had a corresponding specific cellulase production of 5±2 FPU/g-h. Combined with the addition of Triton X-100 for fungal pellet formation and low shear conditions, a stationary phase cellulase production period in excess of 300 h was achieved, with a constant enzyme production rate of 7±1 FPU/g-h. While nitrogen limitation was also effective as a growth limiter, it, however, also prevented cellulase production.
authors.affiliation: Department of Chemical and Biomolecular Engineering, The University of Akron, Akron, OH 44325, USA.
authors.forename: Nicholas V
authors.initials: NV
authors.lastname: Callow
authors.affiliation: Department of Chemical and Biomolecular Engineering, The University of Akron, Akron, OH 44325, USA.
authors.forename: Christopher S
authors.initials: CS
authors.lastname: Ray
authors.affiliation: Department of Chemical and Biomolecular Engineering, The University of Akron, Akron, OH 44325, USA.
authors.forename: Matthew A
authors.initials: MA
authors.lastname: Kelbly
authors.affiliation: Department of Chemical and Biomolecular Engineering, The University of Akron, Akron, OH 44325, USA. Electronic address: LukeJu@uakron.edu.
authors.forename: Lu-Kwang
authors.initials: LK
authors.lastname: Ju
mesh_terms: D000645:Ammonium Sulfate / Q000494:pharmacology; D018533:Biomass; D002480:Cellulase / Q000096:biosynthesis* / Q000302:isolation & purification; D003470:Culture Media / Q000494:pharmacology*; D003902:Detergents; D005285:Fermentation; D005656:Fungal Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D007218:Industrial Microbiology / Q000379:methods; D007785:Lactose / Q000378:metabolism / Q000494:pharmacology; D060847:Metabolic Engineering / Q000379:methods; D009172:Mycology / Q000379:methods*; D009584:Nitrogen / Q000378:metabolism*; D017830:Octoxynol; D010710:Phosphates / Q000494:pharmacology; D010758:Phosphorus / Q000378:metabolism*; D011189:Potassium Chloride / Q000494:pharmacology; D012212:Rheology; D014242:Trichoderma / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development
chemical_list: D003470:Culture Media; D003902:Detergents; D005656:Fungal Proteins; D010710:Phosphates; D010758:Phosphorus; D011189:Potassium Chloride; D017830:Octoxynol; D002480:Cellulase; D007785:Lactose; D009584:Nitrogen; D000645:Ammonium Sulfate
keywords: Cellulase; Nitrogen; Pellet; Phosphorus; Trichoderma reesei