title: Two-Dimensional Micro-TLC Phenolic Fingerprints of Selected Mentha sp. on Cyano-Bonded Polar Stationary Phase.
abstract: Micro-thin-layer chromatography in two-dimensional (2D-mTLC) mode in normal- (NP) and reversed-phase (RP) systems by use of cyanopropyl-bonded stationary phases was applied to make fingerprints of 11 species of Mentha genus and two finished pharmaceutical products. Non-aqueous eluents were used in the NP systems. Mixtures of acetonitrile with water and methanol with water were used in the RP chromatographic systems. Optimization of one-dimensional systems was performed by determining RM vs. composition of mobile phase dependencies for standards occurring in various Mentha sp. On the basis of these dependencies, the most selective chromatographic systems for each run were chosen. Then most selective eluents were applied to optimize two-dimensional systems by creating RF in NP systems vs. RF in RP systems correlations. The best two-dimensional systems were chosen on the basis of R(2) values for RF vs. RF correlations (the lowest values of R(2) coefficients). The 2D-mTLC optimized systems were applied to separate phenolic compounds and make fingerprints of the examined plant materials.
authors.affiliation: Department of Inorganic Chemistry, Medical University of Lublin, Chodźki 4A St., Lublin 20-093, Poland mirek.hawryl@umlub.pl.
authors.forename: Mirosław A
authors.initials: MA
authors.lastname: Hawrył
authors.affiliation: Department of Inorganic Chemistry, Medical University of Lublin, Chodźki 4A St., Lublin 20-093, Poland.
authors.forename: Małgorzata A
authors.initials: MA
authors.lastname: Niemiec
authors.affiliation: Department of Inorganic Chemistry, Medical University of Lublin, Chodźki 4A St., Lublin 20-093, Poland.
authors.forename: Kamil
authors.initials: K
authors.lastname: Słomka
authors.affiliation: Department of Inorganic Chemistry, Medical University of Lublin, Chodźki 4A St., Lublin 20-093, Poland.
authors.forename: Monika
authors.initials: M
authors.lastname: Waksmundzka-Hajnos
authors.affiliation: Botanical Garden of Maria Curie-Skłodowska University in Lublin, Sławinkowska 3, Lublin 20-810, Poland.
authors.forename: Grażyna
authors.initials: G
authors.lastname: Szymczak
mesh_terms: D002855:Chromatography, Thin Layer / Q000295:instrumentation / Q000379:methods*; D027541:Mentha / Q000737:chemistry*; D010636:Phenols / Q000032:analysis*
chemical_list: D010636:Phenols