title: Phycicoccus endophyticussp. nov., an endophytic actinobacterium isolated fromBruguiera gymnorhiza.
abstract: A novel endophytic actinobacterium, designated strain IP6SC6T, was isolated from surface-sterilized bark ofBruguiera gymnorhizacollected from Zhanjiang Mangrove Forest National Nature Reserve in Guangdong, China. Cells of strain IP6SC6Twere Gram-stain-positive, aerobic, non-spore-forming, non-motile rods. Strain IP6SC6Tgrew at 20-42 °C (optimum, 37 °C), at pH 6.0-9.0 (optimum, pH 7.0) and in the presence of 0-8 % (w/v) NaCl (optimum, 0-2 %). Chemotaxonomic analyses showed that the isolate possessedmeso-diaminopimelic acid as the diamino acid of the peptidoglycan, galactose and glucose as whole-cell sugars, and MK-8(H4) as the predominant menaquinone. The major polar lipids were diphosphatidylglycerol, phosphatidylinositol and an unknown lipid. The major fatty acids were iso-C15 : 0, anteiso-C15 : 0, anteiso-C17 : 0and iso-C16 : 0. The G+C content of the genomic DNA was 72.5 mol%. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain IP6SC6Tbelonged to the genusPhycicoccusand shared the highest sequence similarity withPhycicoccus jejuensisNRRL B-24460T(96.97 %). On the basis of phylogenetic analysis and phenotypic and chemotaxonomic characteristics, strain IP6SC6Trepresents a novel species of the genusPhycicoccus, for which the namePhycicoccus endophyticussp. nov. is proposed. The type strain is IP6SC6T( = DSM 100020T= CGMCC 4.7300T).
authors.affiliation: Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, PRChina.
authors.forename: Shao-Wei
authors.initials: SW
authors.lastname: Liu
authors.affiliation: Faculty of Basic Medical Sciences, Guilin Medical University, Guilin 541004, PRChina.
authors.forename: Min
authors.initials: M
authors.lastname: Xu
authors.affiliation: Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, PRChina.
authors.forename: Li
authors.initials: L
authors.lastname: Tuo
authors.affiliation: Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, PRChina.|College of Laboratory Medical Science, Hebei North University, Zhangjiakou 075000, PRChina.
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Li
authors.affiliation: Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, PRChina.
authors.forename: Lin
authors.initials: L
authors.lastname: Hu
authors.affiliation: Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, PRChina.
authors.forename: Li
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Chemistry, Johns Hopkins University, Baltimore, MD 21218, USA.
authors.forename: Rong-Feng
authors.initials: RF
authors.lastname: Li
authors.affiliation: Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, PRChina.
authors.forename: Cheng-Hang
authors.initials: CH
authors.lastname: Sun