title: The microtubule catastrophe promoter Sentin delays stable kinetochore-microtubule attachment in oocytes.
abstract: The critical step in meiosis is to attach homologous chromosomes to the opposite poles. In mouse oocytes, stable microtubule end-on attachments to kinetochores are not established until hours after spindle assembly, and phosphorylation of kinetochore proteins by Aurora B/C is responsible for the delay. Here we demonstrated that microtubule ends are actively prevented from stable attachment to kinetochores until well after spindle formation in Drosophila melanogaster oocytes. We identified the microtubule catastrophe-promoting complex Sentin-EB1 as a major factor responsible for this delay. Without this activity, microtubule ends precociously form robust attachments to kinetochores in oocytes, leading to a high proportion of homologous kinetochores stably attached to the same pole. Therefore, regulation of microtubule ends provides an alternative novel mechanism to delay stable kinetochore-microtubule attachment in oocytes.
authors.affiliation: Wellcome Trust Centre for Cell Biology, School of Biological Sciences, The University of Edinburgh, Edinburgh EH9 3BF, Scotland, UK.
authors.forename: A Agata
authors.initials: AA
authors.lastname: Głuszek
authors.affiliation: Wellcome Trust Centre for Cell Biology, School of Biological Sciences, The University of Edinburgh, Edinburgh EH9 3BF, Scotland, UK.
authors.forename: C Fiona
authors.initials: CF
authors.lastname: Cullen
authors.affiliation: Division of Biological Science, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8602, Japan.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Li
authors.affiliation: Waksman Institute, Rutgers University, Piscataway, NJ 08854.
authors.forename: Rachel A
authors.initials: RA
authors.lastname: Battaglia
authors.affiliation: Waksman Institute, Rutgers University, Piscataway, NJ 08854.
authors.forename: Sarah J
authors.initials: SJ
authors.lastname: Radford
authors.affiliation: Wellcome Trust Centre for Cell Biology, School of Biological Sciences, The University of Edinburgh, Edinburgh EH9 3BF, Scotland, UK.
authors.forename: Mariana F
authors.initials: MF
authors.lastname: Costa
authors.affiliation: Waksman Institute, Rutgers University, Piscataway, NJ 08854.
authors.forename: Kim S
authors.initials: KS
authors.lastname: McKim
authors.affiliation: Division of Biological Science, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8602, Japan.
authors.forename: Gohta
authors.initials: G
authors.lastname: Goshima
authors.affiliation: Wellcome Trust Centre for Cell Biology, School of Biological Sciences, The University of Edinburgh, Edinburgh EH9 3BF, Scotland, UK h.ohkura@ed.ac.uk.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Ohkura
mesh_terms: D000818:Animals; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster; D018386:Kinetochores / Q000378:metabolism*; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism*; D008870:Microtubules / Q000378:metabolism*; D009865:Oocytes / Q000166:cytology* / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D013997:Time Factors
chemical_list: D029721:Drosophila Proteins; D008869:Microtubule-Associated Proteins; C561110:Ssp2 protein, Drosophila