title: Identification of cDNA sequences and alternative splicing patterns of canine AMEL genes (AMELX and AMELY).
abstract: Amelogenin is a major protein of the developing enamel matrix. There are two amelogenin genes (AMELX and AMELY) located on the X and Y chromosomes, respectively, in dogs. In the present study, we characterized full-length cDNAs and alternative splicing patterns of the AMEL genes in the tooth tissue of a dog by 5'- and 3'-rapid amplification of cDNA ends and AMEL-specific RT-PCR. Sequence analysis revealed that the coding regions of AMELX and AMELY were 579 and 576 bp (accession Nos. KP244310 and KP244311), respectively. The coding sequence of AMELX had 95.1% identity to that of AMELY. The AMEL genes on X and Y chromosomes were both expressed in developing tooth tissue. Eight different alternatively spliced transcripts were identified, five from AMELX and three from AMELY.
authors.affiliation: College of Animal Science, Jilin University, Changchun, China.
authors.forename: S Q
authors.initials: SQ
authors.lastname: Yan
authors.affiliation: College of Animal Science, Jilin University, Changchun, China.
authors.forename: Y M
authors.initials: YM
authors.lastname: Li
authors.affiliation: College of Animal Science, Jilin University, Changchun, China.
authors.forename: C Y
authors.initials: CY
authors.lastname: Bai
authors.affiliation: College of Animal Science, Jilin University, Changchun, China.
authors.forename: P C
authors.initials: PC
authors.lastname: Guo
authors.affiliation: College of Animal Science, Jilin University, Changchun, China.
authors.forename: S
authors.initials: S
authors.lastname: Si
authors.affiliation: College of Animal Science and Veterinary Medicine, Qingdao Agricultural University, Qingdao, China.
authors.forename: J H
authors.initials: JH
authors.lastname: Sun
authors.affiliation: College of Animal Science, Jilin University, Changchun, China.
authors.forename: Z H
authors.initials: ZH
authors.lastname: Zhao
mesh_terms: D017398:Alternative Splicing; D053523:Amelogenin / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics; D004285:Dogs; D008969:Molecular Sequence Data; D016415:Sequence Alignment
chemical_list: D053523:Amelogenin; D018076:DNA, Complementary