title: Hyaluronic acid concentration-mediated changes in structure and function of porous carriers for corneal endothelial cell sheet delivery.
abstract: In this study, the effects of hyaluronic acid (HA) concentrations (0.05-1.25wt.%) on the properties of porous carriers for corneal endothelial tissue engineering were investigated. The pore size and porosity gradually increased with decreasing solid content. However, at relatively low HA concentration (i.e., 0.05wt.%), the material samples contained small interior pores and a dense surface skin layer, probably due to no gas bubble effect on the stirring processing of porous microstructures of freeze-dried polysaccharide hydrogels. The carriers prepared from 0.25wt.% HA solution had the highest freezable water content and oxygen and glucose permeability among the samples evaluated. Results of cell viability assays and quantitative real-time reverse transcription polymerase chain reaction analyses showed that the HA concentration-related alteration of porous microstructure dictates the compatibility of biopolymer carriers with corneal endothelial cell (CEC) cultures. In vivo studies demonstrated that the CEC sheet/HA carrier construct implants are therapeutically efficacious in the reconstruction of endothelial scrape-wounded corneas. It is concluded that the polysaccharide concentration is the major factor for affecting the processing of carriers and their structure and function. Porous hydrogels prepared from 0.25wt.% HA solution are capable of delivering bioengineered CEC sheets to the posterior surface of cornea.
authors.affiliation: Institute of Biochemical and Biomedical Engineering, Chang Gung University, Taoyuan, Taiwan 33302, Republic of China; Biomedical Engineering Research Center, Chang Gung University, Taoyuan, Taiwan 33302, Republic of China; Molecular Medicine Research Center, Chang Gung University, Taoyuan, Taiwan 33302, Republic of China; Center for Tissue Engineering, Chang Gung Memorial Hospital, Taoyuan, Taiwan 33305, Republic of China. Electronic address: jylai@mail.cgu.edu.tw.
authors.forename: Jui-Yang
authors.initials: JY
authors.lastname: Lai
mesh_terms: D000818:Animals; D003315:Cornea; D065306:Corneal Injuries / Q000628:therapy*; D042783:Endothelial Cells / Q000166:cytology / Q000637:transplantation*; D006820:Hyaluronic Acid / Q000737:chemistry*; D016062:Porosity; D011817:Rabbits
chemical_list: D006820:Hyaluronic Acid
keywords: Cell delivery; Corneal endothelial reconstruction; Hyaluronic acid concentration; Porous carrier