title: Does hybridization with a widespread congener threaten the long-term persistence of the Eastern Alpine rare local endemic Knautia carinthiaca?
abstract: Interspecific hybridization, especially when regularly followed by backcrossing (i.e., introgressive hybridization), conveys a substantial risk for many endangered organisms. This is particularly true for narrow endemics occurring within distributional ranges of widespread congeners. An excellent example is provided by the plant genus Knautia (Caprifoliaceae): Locally endemic K. carinthiaca is reported from two isolated populations in southern Austria situated within an area predominantly occupied by widespread K. arvensis. While K. carinthiaca usually inhabits low-competition communities on rocky outcrops, K. arvensis occurs mainly in dry to mesic managed grasslands, yet both species can coexist in marginal environments and were suspected to hybridize. Flow cytometry revealed that diploid K. carinthiaca only occurs at its locus classicus, whereas the second locality is inhabited by the morphologically similar but tetraploid K. norica. In the, therefore, single population of K. carinthiaca, flow cytometry and AFLP fingerprinting showed signs of introgressive hybridization with diploid K. arvensis. Hybridization patterns were also reflected in intermediate habitat preferences and morphology of the hybrids. Environmental barriers to gene flow seem to prevent genetic erosion of K. carinthiaca individuals from the core ecological niches, restricting most introgressed individuals to peripheral habitats. Efficient conservation of K. carinthiaca will require strict protection of its habitat and ban on forest clear cuts in a buffer zone to prevent invasion of K. arvensis. We demonstrate the large potential of multidisciplinary approaches combining molecular, cytometric, and ecological tools for a reliable inventory and threat assessment of rare species.
authors.affiliation: Department of Botany Faculty of Science Charles University in Prague Benátská 2 CZ-128 00 Prague Czech Republic ; Institute of Botany The Czech Academy of Sciences Zámek 1 CZ-252 43 Průhonice Czech Republic.
authors.forename: Martin
authors.initials: M
authors.lastname: Čertner
authors.affiliation: Department of Botany Faculty of Science Charles University in Prague Benátská 2 CZ-128 00 Prague Czech Republic ; Institute of Botany The Czech Academy of Sciences Zámek 1 CZ-252 43 Průhonice Czech Republic ; National Centre for Biosystematics Natural History Museum University of Oslo NO-0318 Oslo Norway.
authors.forename: Filip
authors.initials: F
authors.lastname: Kolář
authors.affiliation: Institute of Botany University of Innsbruck Sternwartestraße 15 6020 Innsbruck Austria.
authors.forename: Peter
authors.initials: P
authors.lastname: Schönswetter
authors.affiliation: Institute of Botany University of Innsbruck Sternwartestraße 15 6020 Innsbruck Austria.
authors.forename: Božo
authors.initials: B
authors.lastname: Frajman
keywords: Eastern Alps; habitat segregation; human‐induced landscape changes; introgression; relict; steno‐endemic species