title: Hybrid Amyloid Membranes for Continuous Flow Catalysis.
abstract: Amyloid fibrils are promising nanomaterials for technological applications such as biosensors, tissue engineering, drug delivery, and optoelectronics. Here we show that amyloid-metal nanoparticle hybrids can be used both as efficient active materials for wet catalysis and as membranes for continuous flow catalysis applications. Initially, amyloid fibrils generated in vitro from the nontoxic β-lactoglobulin protein act as templates for the synthesis of gold and palladium metal nanoparticles from salt precursors. The resulting hybrids possess catalytic features as demonstrated by evaluating their activity in a model catalytic reaction in water, e.g., the reduction of 4-nitrophenol into 4-aminophenol, with the rate constant of the reduction increasing with the concentration of amyloid-nanoparticle hybrids. Importantly, the same nanoparticles adsorbed onto fibrils surface show improved catalytic efficiency compared to the same unattached particles, pointing at the important role played by the amyloid fibril templates. Then, filter membranes are prepared from the metal nanoparticle-decorated amyloid fibrils by vacuum filtration. The resulting membranes serve as efficient flow catalysis active materials, with a complete catalytic conversion achieved within a single flow passage of a feeding solution through the membrane.
authors.affiliation: Department of Health Sciences and Technology, ETH Zurich , Schmelzbergstrasse 9, 8092 Zurich, Switzerland.
authors.forename: Sreenath
authors.initials: S
authors.lastname: Bolisetty
authors.affiliation: Department of Health Sciences and Technology, ETH Zurich , Schmelzbergstrasse 9, 8092 Zurich, Switzerland.
authors.forename: Mario
authors.initials: M
authors.lastname: Arcari
authors.affiliation: Department of Health Sciences and Technology, ETH Zurich , Schmelzbergstrasse 9, 8092 Zurich, Switzerland.
authors.forename: Jozef
authors.initials: J
authors.lastname: Adamcik
authors.affiliation: Department of Health Sciences and Technology, ETH Zurich , Schmelzbergstrasse 9, 8092 Zurich, Switzerland.
authors.forename: Raffaele
authors.initials: R
authors.lastname: Mezzenga
mesh_terms: D000682:Amyloid / Q000737:chemistry*; D001672:Biocompatible Materials / Q000737:chemistry; D002384:Catalysis; D055767:Immobilized Proteins / Q000737:chemistry; D007782:Lactoglobulins / Q000737:chemistry; D008567:Membranes, Artificial; D053768:Metal Nanoparticles / Q000737:chemistry; D018625:Microscopy, Atomic Force; D064347:Microscopy, Electrochemical, Scanning
chemical_list: D000682:Amyloid; D001672:Biocompatible Materials; D055767:Immobilized Proteins; D007782:Lactoglobulins; D008567:Membranes, Artificial