title: A transgenic, visual screenable marker for soybean seeds.
abstract: Most soybean cultivars produce buff colored seeds due to a seed coat specific siRNA mechanism. This phenomenon is specifically limited to the seed coat and produces a strong visual effect, thus, a strategy to evade the silencing was used to produce a maternal transgenic marker for soybeans. Expression of a rice chalcone synthase transgene with little DNA sequence homology to the soybean siRNAs resulted in dark colored seed coats. This phenotype is the result of anthocyanin pigment production and does not appear to affect other tissues. This novel approach for producing an easily scored transgenic marker for soybean will facilitate high-throughput screening and analysis of transgenic soybean.
authors.affiliation: Center for Applied Genetic Technologies, University of Georgia, 111 Riverbend Rd, Athens, GA, 30602, USA.|Institute for Plant Breeding, Genetics and Genomics, University of Georgia, 111 Riverbend Rd, Athens, GA, 30602, USA.
authors.forename: Lisa
authors.initials: L
authors.lastname: Kanizay
authors.affiliation: Center for Applied Genetic Technologies, University of Georgia, 111 Riverbend Rd, Athens, GA, 30602, USA.|Institute for Plant Breeding, Genetics and Genomics, University of Georgia, 111 Riverbend Rd, Athens, GA, 30602, USA.
authors.forename: Thomas
authors.initials: T
authors.lastname: Jacobs
authors.affiliation: Department of Biology and Geology, University of South Carolina Aiken, 471 University Parkway, Aiken, SC, 29801, USA. nathanh@usca.edu.
authors.forename: C Nathan
authors.initials: CN
authors.lastname: Hancock
mesh_terms: D000217:Acyltransferases / Q000096:biosynthesis / Q000235:genetics*; D015415:Biomarkers; D018506:Gene Expression Regulation, Plant; D020868:Gene Silencing; D012275:Oryza / Q000235:genetics; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000254:growth & development; D034741:RNA, Small Interfering / Q000235:genetics; D012639:Seeds / Q000235:genetics* / Q000254:growth & development; D013025:Glycine max / Q000235:genetics* / Q000254:growth & development
chemical_list: D015415:Biomarkers; D034741:RNA, Small Interfering; D000217:Acyltransferases; C020141:flavanone synthetase
keywords: Chalcone synthase; RNA silencing; Seed coat; Soybean; Visual screening