title: [Purification Technology Optimization for Saponins from Ziziphi Spinosae Semen with Macroporous Adsorption Resin by Box-Behnken Design-Response Surface Methodology].
abstract: OBJECTIVE
  To compare the purification effect of saponins from Ziziphi Spinosae Semen with different types of macroporous adsorption resin, and to optimize its purification technology.
  
  
  METHODS
  The type of macroporous resins was optimized by static adsorption method. The optimum technological conditions of saponins from Ziziphi Spinosae Semen was screened by single factor test and Box-Behnken Design-Response Surface Methodology.
  
  
  RESULTS
  AB-8 macroporous resin had better purification effect of total saponins than other resins, optimum technological parameters were as follows: column height-diameter ratio was 5: 1, the concentration of sample solution was 2. 52 mg/mL, resin adsorption quantity was 8. 915 mg/g, eluted by 3 BV water, flow rate of adsorption and elution was 2 BV/h, elution solvent was 75% ethanol, elution solvent volume was 5 BV.
  
  
  CONCLUSIONS
  AB-8 macroporous resin has a good purification effect on jujuboside A. The optimized technology is stable and feasible.
authors.forename: Hui-ru
authors.initials: HR
authors.lastname: Zhao
authors.forename: Zao
authors.initials: Z
authors.lastname: Ren
authors.forename: Chun-ye
authors.initials: CY
authors.lastname: Liu
mesh_terms: D000327:Adsorption; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D000431:Ethanol; D012116:Resins, Plant / Q000737:chemistry*; D012503:Saponins / Q000302:isolation & purification*; D012639:Seeds / Q000737:chemistry*; D012997:Solvents; D013678:Technology, Pharmaceutical; D014867:Water; D031957:Ziziphus / Q000737:chemistry*
chemical_list: D004365:Drugs, Chinese Herbal; D012116:Resins, Plant; D012503:Saponins; D012997:Solvents; D014867:Water; D000431:Ethanol; C087808:jujuboside A