title: [Determination of Amino Acids in Pearl Powder and Conch Powder from Different Origins by HPLC with Pre-column Derivation].
abstract: OBJECTIVE
  To establish an analysis method for determination of amino acids in pearl powder and conch powder from different origins by HPLC with pre-column derivation.
  
  
  METHODS
  Hydrolytic amino acids were extracted by acid hydrolysis method,then derivatized with phenyl isothiocyanate (PITC). And the samples were analyzed on a C, column with gradient elution of 0.1 mol/L sodium acetate solution (adjusted to pH 6. 5) (A) and acetonitrile (B) at the flow rate of 1. 0 mL/min. The detected wavelength was 254 nm.
  
  
  RESULTS
  Amino acids derivative solution remained stable in 24 hours. The response was linear for seven amino acids with a correlation coefficient r >0. 9995. The average recoveries were 93. 16% - 103. 31%.
  
  
  CONCLUSIONS
  The method is sensitive with good accuracy and repeatability, which is useful for the determination of amino acids in pearl powder and conch powder from differei origins.
authors.forename: Jia
authors.initials: J
authors.lastname: Chen
authors.forename: Feng
authors.initials: F
authors.lastname: Wei
authors.forename: Xian-long
authors.initials: XL
authors.lastname: Cheng
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Shuang-cheng
authors.initials: SC
authors.lastname: Ma
mesh_terms: D000596:Amino Acids / Q000032:analysis*; D060105:Animal Shells / Q000737:chemistry*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D008974:Mollusca; D011208:Powders
chemical_list: D000596:Amino Acids; D011208:Powders