title: One-step detection of pathogens and cancer biomarkers by the naked eye based on aggregation of immunomagnetic beads.
abstract: This report shows that immunomagnetic beads (IMBs) can act as the optical readout for assays, in addition to serving as the carrier for purification/separation. Under the influence of an external magnet, IMBs are attracted to coat one side of a test tube. IMBs specifically bound to targets can form a narrow brown stripe, whereas free IMBs will form a diffuse, yellow coating on the side of the test tube. Target analytes can aggregate initially dispersed IMBs in a sample concentration-dependent manner, yielding a color change from yellow to brown that can be seen with the naked eye. This assay combines the convenience of a lateral flow assay, allowing a one-step assay to finish within 15 min, with the sensitivity of an enzyme-linked immonosorbent assay.
authors.affiliation: Beijing Engineering Research Center for BioNanotechnology and CAS Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, National Center for NanoScience and Technology, Beijing 100190, China. xingyujiang@nanoctr.cn.
authors.forename: Yiping
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Beijing Engineering Research Center for BioNanotechnology and CAS Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, National Center for NanoScience and Technology, Beijing 100190, China. xingyujiang@nanoctr.cn.
authors.forename: Yunlei
authors.initials: Y
authors.lastname: Xianyu
authors.affiliation: Beijing Engineering Research Center for BioNanotechnology and CAS Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, National Center for NanoScience and Technology, Beijing 100190, China. xingyujiang@nanoctr.cn.
authors.forename: Jiashu
authors.initials: J
authors.lastname: Sun
authors.affiliation: Beijing Engineering Research Center for BioNanotechnology and CAS Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, National Center for NanoScience and Technology, Beijing 100190, China. xingyujiang@nanoctr.cn.
authors.forename: Yajing
authors.initials: Y
authors.lastname: Niu
authors.affiliation: Beijing Institute for Tropical Medicine, Beijing Friendship Hospital, Capital Medical University, 95, Yongan Road, Xicheng District, Beijing, 100050, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Beijing Engineering Research Center for BioNanotechnology and CAS Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, National Center for NanoScience and Technology, Beijing 100190, China. xingyujiang@nanoctr.cn.
authors.forename: Xingyu
authors.initials: X
authors.lastname: Jiang
mesh_terms: D014408:Biomarkers, Tumor / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D004926:Escherichia coli / Q000302:isolation & purification; D006801:Humans; D018189:Immunomagnetic Separation / Q000379:methods*; D008280:Magnetics; D036103:Nanotechnology / Q000379:methods; D009369:Neoplasms / Q000175:diagnosis* / Q000235:genetics; D055095:Optics and Photonics; D019095:Point-of-Care Systems; D012475:Salmonella / Q000302:isolation & purification; D012760:Shigella / Q000302:isolation & purification; D013139:Spirillum / Q000302:isolation & purification; D013211:Staphylococcus aureus / Q000302:isolation & purification; D013314:Stress, Mechanical
chemical_list: D014408:Biomarkers, Tumor