title: Impact of methionine on a partial-nitritation biofilter.
abstract: It has been demonstrated that an anaerobic digestion process cannot attain an efficient removal of several amino acids, with methionine being one of the most persistent of these. Thus, the effect that methionine amino acid has over the partial-nitritation process with fixed-biofilm configuration in terms of performance and bacterial community dynamics has been investigated. With respect to the performance with no addition, 100 mg/L methionine loading decreased ammonium oxidation efficiency in 60% and 100% at concentrations of 300 and 500 mg/L methionine, respectively. Bacterial biomass sharply increased by 30, 65, and 230% with the addition of 100, 300, and 500 mg/L methionine, respectively. Bacterial community analysis showed that methionine addition supported the proliferation of a diversity of heterotrophic genera, such as Lysobacter and Micavibrio, and reduced the relative abundance of ammonium oxidizing genus Nitrosomonas. This research shows that the addition of methionine affects the performance of the partial-nitritation process. In this sense, amino acids can pose a threat for the of partial-nitritation process treating anaerobic digester supernatant at full-scale implementation.
authors.affiliation: Department of Civil Engineering, School of Civil Engineering, Campus of Fuentenueva, University of Granada, s/n, 18071, Granada, Spain. agon@ugr.es.
authors.forename: Alejandro
authors.initials: A
authors.lastname: Gonzalez-Martinez
authors.affiliation: Institute of Water Research, University of Granada, Calle Ramon y Cajal 4, 18071, Granada, Spain.
authors.forename: Alejandro
authors.initials: A
authors.lastname: Rodriguez-Sanchez
authors.affiliation: Department of Civil Engineering, School of Civil Engineering, Campus of Fuentenueva, University of Granada, s/n, 18071, Granada, Spain.
authors.forename: Maria Jesus
authors.initials: MJ
authors.lastname: Garcia-Ruiz
authors.affiliation: Department of Civil Engineering, School of Civil Engineering, Campus of Fuentenueva, University of Granada, s/n, 18071, Granada, Spain.
authors.forename: Francisco
authors.initials: F
authors.lastname: Osorio
authors.affiliation: Institute of Water Research, University of Granada, Calle Ramon y Cajal 4, 18071, Granada, Spain.
authors.forename: Jesus
authors.initials: J
authors.lastname: Gonzalez-Lopez
mesh_terms: D064751:Ammonium Compounds / Q000032:analysis / Q000378:metabolism; D000693:Anaerobiosis; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D018533:Biomass; D019149:Bioreactors / Q000382:microbiology*; D008715:Methionine / Q000378:metabolism / Q000494:pharmacology*; D008962:Models, Theoretical; D058465:Nitrification / Q000187:drug effects; D009584:Nitrogen / Q000032:analysis* / Q000378:metabolism; D009606:Nitrosomonas / Q000254:growth & development / Q000378:metabolism; D010084:Oxidation-Reduction; D014874:Water Pollutants, Chemical / Q000378:metabolism / Q000494:pharmacology*; D018508:Water Purification / Q000379:methods*
chemical_list: D064751:Ammonium Compounds; D014874:Water Pollutants, Chemical; D008715:Methionine; D009584:Nitrogen
keywords: Autotrophic nitrogen removal; Biofilters; Bioreactors; Illumina MiSeq; Methionine; Partial nitritation; Wastewater treatment