title: Zepto-molar electrochemical detection of Brucella genome based on gold nanoribbons covered by gold nanoblooms.
abstract: Gold nanoribbons covered by gold nanoblooms were sonoelectrodeposited on a polycrystalline gold surface at -1800 mV (vs. AgCl) with the assistance of ultrasound and co-occurrence of the hydrogen evolution reaction. The nanostructure, as a transducer, was utilized to immobilize a Brucella-specific probe and fabrication of a genosensor, and the process of immobilization and hybridization was detected by electrochemical methods, using methylene blue as a redox marker. The proposed method for detection of the complementary sequence, sequences with base-mismatched (one-, two- and three-base mismatches), and the sequence of non-complementary sequence was assayed. The fabricated genosensor was evaluated for the assay of the bacteria in the cultured and human samples without polymerase chain reactions (PCR). The genosensor could detect the complementary sequence with a calibration sensitivity of 0.40 μA dm(3) mol(-1), a linear concentration range of 10 zmol dm(-3) to 10 pmol dm(-3), and a detection limit of 1.71 zmol dm(-3).
authors.affiliation: Department of Nanomedicine, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Amid
authors.initials: A
authors.lastname: Rahi
authors.affiliation: Nanomedicine and Nanobiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.|Department of Medical Physics, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Naghmeh
authors.initials: N
authors.lastname: Sattarahmady
authors.affiliation: Department of Nanomedicine, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran.|Nanomedicine and Nanobiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Hossein
authors.initials: H
authors.lastname: Heli
mesh_terms: D001681:Biological Assay / Q000379:methods; D015374:Biosensing Techniques / Q000379:methods; D002002:Brucella / Q000737:chemistry* / Q000235:genetics*; D055664:Electrochemical Techniques / Q000379:methods; D004563:Electrochemistry / Q000379:methods; D004566:Electrodes; D016680:Genome, Bacterial / Q000235:genetics*; D006046:Gold / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D008751:Methylene Blue / Q000737:chemistry; D049329:Nanostructures / Q000737:chemistry*; D037742:Nanotubes, Carbon; D009693:Nucleic Acid Hybridization / Q000235:genetics
chemical_list: D037742:Nanotubes, Carbon; D006046:Gold; D008751:Methylene Blue