title: Complete genomic sequence of barley (Hordeum vulgare) endornavirus (HvEV) determined by next-generation sequencing.
abstract: Endornaviruses are unusual plant-, fungus- and oomycete-infecting viruses with a large, ca 14- to 17-kb linear double-stranded RNA (dsRNA) genome and a persistent lifestyle. The complete genome sequence of an endornavirus from the barley (Hordeum vulgare) Nerz variety was determined from paired Illumina MySeq reads derived from purified dsRNAs. The genome is 14,243 nt long, with 5' and 3' non-coding regions of 207 and 47 nt, respectively. It encodes a single large protein of 4663 amino acids that carries conserved motifs for a methyltransferase, a helicase and an RNA-dependent RNA polymerase. The sequence of Hordeum vulgare endornavirus (HvEV) carries all the hallmarks of a typical member of the genus Endornavirus, with the exception of an UDP-glycosyltransferase motif observed in many, but not all, endornaviral genomes.
authors.affiliation: INRA, UMR 1332 Biologie du Fruit et Pathologie, CS 20032, 33882, Villenave d'Ornon Cedex, France. tc@bordeaux.inra.fr.|Université de Bordeaux, UMR 1332 Biologie du Fruit et Pathologie, CS 20032, 33882, Villenave d'Ornon Cedex, France. tc@bordeaux.inra.fr.
authors.forename: Thierry
authors.identifier: http://orcid.org/0000-0001-9757-1835
authors.initials: T
authors.lastname: Candresse
authors.affiliation: INRA, UMR 1332 Biologie du Fruit et Pathologie, CS 20032, 33882, Villenave d'Ornon Cedex, France.|Université de Bordeaux, UMR 1332 Biologie du Fruit et Pathologie, CS 20032, 33882, Villenave d'Ornon Cedex, France.
authors.forename: Armelle
authors.initials: A
authors.lastname: Marais
authors.affiliation: Dipartimento di Agraria, Università degli Studi di Napoli "Federico II", 80055, Portici, Italy.
authors.forename: Roberto
authors.initials: R
authors.lastname: Sorrentino
authors.affiliation: INRA, UMR 1332 Biologie du Fruit et Pathologie, CS 20032, 33882, Villenave d'Ornon Cedex, France.|Université de Bordeaux, UMR 1332 Biologie du Fruit et Pathologie, CS 20032, 33882, Villenave d'Ornon Cedex, France.
authors.forename: Chantal
authors.initials: C
authors.lastname: Faure
authors.affiliation: INRA, UMR 1332 Biologie du Fruit et Pathologie, CS 20032, 33882, Villenave d'Ornon Cedex, France.|Université de Bordeaux, UMR 1332 Biologie du Fruit et Pathologie, CS 20032, 33882, Villenave d'Ornon Cedex, France.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Theil
authors.affiliation: GEVES, 25 rue Georges Morel, CS 90024, 49071, Beaucouzé Cedex, France.
authors.forename: Valérie
authors.initials: V
authors.lastname: Cadot
authors.affiliation: GEVES, 25 rue Georges Morel, CS 90024, 49071, Beaucouzé Cedex, France.
authors.forename: Mathieu
authors.initials: M
authors.lastname: Rolland
authors.affiliation: GEVES, 25 rue Georges Morel, CS 90024, 49071, Beaucouzé Cedex, France.
authors.forename: Julie
authors.initials: J
authors.lastname: Villemot
authors.affiliation: Institute for Epidemiology and Pathogen Diagnostics, Julius Kuhn-Institute, Federal Research Institute for Cultivated Plants, Erwin-Baur-Straße 27, 06484, Quedlinburg, Germany.
authors.forename: Frank
authors.initials: F
authors.lastname: Rabenstein
mesh_terms: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D016000:Cluster Analysis; D016679:Genome, Viral; D001467:Hordeum / Q000821:virology*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D020815:Polyproteins / Q000235:genetics; D012328:RNA Viruses / Q000235:genetics* / Q000302:isolation & purification; D012367:RNA, Viral / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
chemical_list: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D020815:Polyproteins; D012367:RNA, Viral