title: Enhanced production of optically pure d (-) lactic acid from nutritionally rich Borassus flabellifer sugar and whey protein hydrolysate based-fermentation medium.
abstract: The aim of this study is to optimize the production of optically pure d (-) lactic acid (DLA) employing a cost-effective production medium. Based on the designed biomass approach, Sporolactobacillus inulinus NBRC 13595 was found to exhibit high DLA titer (19.0 g L-1) and optical purity (99.6%). A cost-effective medium was constituted using Palmyra palm jaggery (PJ) from Borassus flabellifer and whey protein hydrolysate (WPH) as carbon and nitrogen sources, respectively. Plackett-Burman design indicated that PJ, WPH, and MnSO4as significant variables influence DLA production. A rotatable central composite design and response surface methodology were used to optimize the PJ and WPH concentrations. A maximum DLA titer (170.14 g L-1) was predicted for 222.24 g L-1of PJ and 11.99 g L-1of WPH, respectively. Fermentation experimental results exhibited a maximum DLA titer (189.0 ± 8.53 g L-1) and productivity (5.25 ± 0.24 g L-1H-1), which is the highest ever reported for DLA production from a renewable feedstock in the batch process. The present investigation substantiates that the potential application of economically viable raw feedstocks (PJ and WPH) for enhanced DLA production, which is attributed to 2.5-fold reduction in DLA production cost compared with conventional medium.
authors.affiliation: BioPAT Laboratory, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, India.
authors.forename: Subbi Rami
authors.initials: SR
authors.lastname: Reddy Tadi
authors.affiliation: BioPAT Laboratory, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, India.
authors.forename: Arun
authors.initials: A
authors.lastname: E V R
authors.affiliation: BioPAT Laboratory, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, India.
authors.forename: Anil Mukund
authors.initials: AM
authors.lastname: Limaye
authors.affiliation: BioPAT Laboratory, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, India.
authors.forename: Senthilkumar
authors.initials: S
authors.lastname: Sivaprakasam
mesh_terms: D028023:Arecaceae / Q000737:chemistry*; D018533:Biomass; D019149:Bioreactors; D002244:Carbon / Q000737:chemistry; D003470:Culture Media; D005285:Fermentation; D019344:Lactic Acid / Q000096:biosynthesis* / Q000737:chemistry; D007778:Lactobacillus; D009584:Nitrogen / Q000737:chemistry; D011492:Protein Hydrolysates / Q000737:chemistry; D000067816:Whey Proteins / Q000737:chemistry*
chemical_list: D003470:Culture Media; D011492:Protein Hydrolysates; D000067816:Whey Proteins; D019344:Lactic Acid; D002244:Carbon; D009584:Nitrogen
keywords: Palmyra palm jaggery; batch fermentation; d (-) lactic acid; medium optimization; renewable feedstock; whey protein hydrolysate