title: Two strategies for the synthesis of the biologically important ATP analogue ApppI, at a multi-milligram scale.
abstract: Two strategies for the synthesis of the ATP (adenosine triphosphate) analogue ApppI [1-adenosin-5'-yl 3-(3-methylbut-3-enyl)triphosphoric acid diester] (1) are described. ApppI is an active metabolite of the mevalonate pathway and thus is of major biological significance. Chemically synthezised ApppI was purified by using triethylammonium bicarbonate as the counter ion in ion-pair chromatography and characterized by (1)H, (13)C, (31)P NMR and MS spectroscopical methods.
authors.affiliation: University of Eastern Finland, School of Pharmacy, Biocenter Kuopio, P.O. Box 1627, FIN-70211, Kuopio, Finland.
authors.forename: Janne
authors.initials: J
authors.lastname: Weisell
authors.affiliation: University of Eastern Finland, School of Pharmacy, Biocenter Kuopio, P.O. Box 1627, FIN-70211, Kuopio, Finland.
authors.forename: Jouko
authors.initials: J
authors.lastname: Vepsäläinen
authors.affiliation: University of Eastern Finland, School of Pharmacy, Biocenter Kuopio, P.O. Box 1627, FIN-70211, Kuopio, Finland.
authors.forename: Petri A
authors.initials: PA
authors.lastname: Turhanen
keywords: ATP analogue; ApppI; HPLC; purification; synthesis