title: Knockdown of a HIF-2α promoter upstream long noncoding RNA impairs colorectal cancer stem cell properties in vitro through HIF-2α downregulation.
abstract: Currently, various long noncoding RNAs (lncRNAs) have been identified as key regulators of multiple cancers. However, cancer stem cell (CSC)-related lncRNAs have rarely been reported. In this study, we found an lncRNA that is a promoter upstream transcript of hypoxia-inducible factor-2α (HIF-2α), and we named it "lncRNA-HIF2PUT". The function of HIF-2α is closely connected with "stem cell-like" properties, and the function of PROMPTs is often associated with the adjacent protein-coding transcripts. Herein, we showed that the expression of lncRNA-HIF2PUT was significantly correlated with HIF-2α in colorectal cancer (CRC) tissues. Knockdown of lncRNA-HIF2PUT blocked the HIF-2α expression and inhibited the CSC properties in CRC cell lines DLD-1 and HT29. LncRNA-HIF2PUTsmall interfering RNA transfection resulted in decreased stemness genes expression, impaired colony formation, and spheroid formation ability, retarded migration, and invasion of the cells. These data suggest that lncRNA-HIF2PUT may be a regulator of HIF-2α and a mediator of CSCs in CRC.
authors.affiliation: Department of Oncology, People's Liberation Army No 161 Hospital, Wuhan, People's Republic of China.
authors.forename: Jie
authors.initials: J
authors.lastname: Yao
authors.affiliation: Cancer Center, Division of Internal Medicine, Chinese PLA General Hospital, Beijing, People's Republic of China.
authors.forename: Jianxiong
authors.initials: J
authors.lastname: Li
authors.affiliation: Cancer Center, Division of Internal Medicine, Chinese PLA General Hospital, Beijing, People's Republic of China.
authors.forename: Peiliang
authors.initials: P
authors.lastname: Geng
authors.affiliation: Department of Oncology, Kunming General Hospital of Chendu Military Command, Kunming, People's Republic of China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Oncology, Kunming General Hospital of Chendu Military Command, Kunming, People's Republic of China.
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
authors.affiliation: Cancer Center, Division of Internal Medicine, Chinese PLA General Hospital, Beijing, People's Republic of China.
authors.forename: Yunfeng
authors.initials: Y
authors.lastname: Zhu
keywords: HIF-2α; colorectal cancer; long noncoding RNA; stem cell properties