title: [Applylication of new type combined fragments: nrDNA ITS+ nad 1-intron 2 for identification of Dendrobium species of Fengdous].
abstract: In this study, 17 kinds of Dendrobium species of Fengdous including 39 individuals were collected from 4 provinces. Mitochondrial gene sequences co I, nad 5, nad 1-intron 2 and chloroplast gene sequences rbcL, matK amd psbA-trnH were amplified from these materials, as well as nrDNA ITS. Furthermore, suitable sequences for identification of Dendrobium species of Fengdous were screened by K-2-P and P-distance. The results showed that during the mentioned 7 sequences, nrDNA ITS, nad 1-intron 2 and psbA-trnH which had a high degree of variability could be used to identify Dendrobium species of Fengdous. However, single fragment could not be used to distinguish D. moniliforme and D. huoshanense. Moreover, compared to other combined fragments, new type combined fragments nrDNA ITS+nad 1-intron 2 was more effective in identifying the original plants of Dendrobium species and could be used to identify D. huoshanense and D. moniliforme. Besides, according to the UPGMA tree constructed with nrDNA ITS+nad 1-intron 2, 3 inspected Dendrobium plants were identified as D. huoshanense, D. moniliforme and D. officinale, respectively. This study identified Dendrobium species of Fengdous by combined fragments nrDNA ITS+nad 1-intron 2 for the first time, which provided a more effective basis for identification of Dendrobium species. And this study will be helpful for regulating the market of Fengdous.
authors.forename: Li-xia
authors.initials: LX
authors.lastname: Geng
authors.forename: Rui
authors.initials: R
authors.lastname: Zheng
authors.forename: Jie
authors.initials: J
authors.lastname: Ren
authors.forename: Zhi-tao
authors.initials: ZT
authors.lastname: Niu
authors.forename: Yu-long
authors.initials: YL
authors.lastname: Sun
authors.forename: Qing-yun
authors.initials: QY
authors.lastname: Xue
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Xiao-yu
authors.initials: XY
authors.lastname: Ding
mesh_terms: D018744:DNA, Plant / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics*; D031667:Dendrobium / Q000145:classification* / Q000235:genetics; D061125:Genes, Chloroplast; D017343:Genes, Plant; D007438:Introns; D010946:Plants, Medicinal / Q000145:classification / Q000235:genetics
chemical_list: D018744:DNA, Plant; D021903:DNA, Ribosomal Spacer