title: [Inhibition of osthole for resorption of rats femur tissue in vitro].
abstract: OBJECTIVE
  To investigate osthole effect on femoral tissue resorption activity of rat in vitro.
  
  
  METHODS
  Six SD rats weighted (80 ± 5) g were used to isolate and culture femoral tissue (diaphyses and metaphysis) in vitro. The cultured tissue were devided into control group, estradiol group and osthole group. The femoral tissue was treated with final concentration of 1 x 10(-5) mol/L osthole and 1 x 10(-8) mol/L estradiol culture in vitro at 48 hours after cultured. Tartrate-resistant acid phosphatase (StrACP) activity, glucose and Lactic acid content, StrACP, MCSF (Macrophage colony stimulating factor) and CTSK (Cathepsin K) mRNA was detected by Real-Time RT-PCR were detected.
  
  
  RESULTS
  Concetration of Alkaline phosphatase activity were 2226 and 2498 in 1 x 10(-5) mol/L osthole and 1 x 10(-8) mol/L estradiol respectively. As compared with control group, the activity of StrACP of 1 x 10(-5) mol/L osthole and 1 x 10(-8) mol/L estradiol were inhibited at 6, 9, 12 days (P < 0.05); under treatment of in l x 10(-5) mol/L osthole, the content of Lactic acid were increased and the content of glucose were decreased at 3, 6, 9 days (P < 0.05); StrACP, MCSF and CTSK mRNA expression level were inhibited at 6, 9 days (P < 0.05).
  
  
  CONCLUSIONS
  Osthole can inhibit bone resorption and raise the level of nutrition metabolism of femurs tissue.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhou
authors.forename: Xue-mei
authors.initials: XM
authors.lastname: Ren
authors.forename: Xiao-ni
authors.initials: XN
authors.lastname: Ma
authors.forename: Yu-hai
authors.initials: YH
authors.lastname: Gao
authors.forename: Li-juan
authors.initials: LJ
authors.lastname: Yan
authors.forename: Wen-gui
authors.initials: WG
authors.lastname: Shi
authors.forename: Ke-ming
authors.initials: KM
authors.lastname: Chen
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D001862:Bone Resorption / Q000517:prevention & control*; D003374:Coumarins / Q000494:pharmacology*; D004958:Estradiol / Q000494:pharmacology; D005269:Femur / Q000187:drug effects*; D005947:Glucose / Q000032:analysis; D019344:Lactic Acid / Q000032:analysis; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D003374:Coumarins; D019344:Lactic Acid; D004958:Estradiol; D000135:Acid Phosphatase; D005947:Glucose; C046627:osthol