title: Tissue expression analysis, cloning, and characterization of the 5'-regulatory region of the bovine fatty acid binding protein 4 gene.
abstract: Fatty acid binding protein 4 (FABP4) is a member of the FABP family of proteins that bind fatty acids and play important roles in fatty acid uptake and intracellular transport. In the present study, we cloned the 5'-regulatory region of bovine and identified its transcription initiation sites. Sequence comparative analysis demonstrated amino acids and promoter sequences of were highly conservative in mammals. Real-time PCR analysis revealed the products of bovine were very highly expressed in subcutaneous adipose tissue. Serial deletion constructs of the bovine 5'-regulatory region evaluated in a dual-luciferase reporter assay showed that 209 bp upstream from the transcription initiation site was its core promoter. An electrophoretic mobility shift assay combined with a site-directed mutation experiment indicated that peroxisome proliferator activated receptor gamma (PPARγ), CCAAT/enhancer-binding protein β (C/EBPβ), and sterol regulatory element-binding protein (SREBP) were important transcription factors for bovine . These results provide an important basis for further understanding the regulation of bovine .
authors.forename: A
authors.initials: A
authors.lastname: Li
authors.forename: Z
authors.initials: Z
authors.lastname: Zhao
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
authors.forename: C
authors.initials: C
authors.lastname: Fu
authors.forename: M
authors.initials: M
authors.lastname: Wang
authors.forename: L
authors.initials: L
authors.lastname: Zan
mesh_terms: D000818:Animals; D001483:Base Sequence; D022762:CCAAT-Enhancer-Binding Proteins / Q000235:genetics; D002417:Cattle; D003001:Cloning, Molecular; D024202:Electrophoretic Mobility Shift Assay; D050556:Fatty Acid-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000502:physiology*; D009154:Mutation; D011401:Promoter Regions, Genetic; D060888:Real-Time Polymerase Chain Reaction; D012045:Regulatory Sequences, Nucleic Acid; D051780:Sterol Regulatory Element Binding Protein 1 / Q000378:metabolism
chemical_list: D022762:CCAAT-Enhancer-Binding Proteins; D050556:Fatty Acid-Binding Proteins; D051780:Sterol Regulatory Element Binding Protein 1