title: [The study of anticoagulants selection in platelet-rich plasma preparation].
abstract: OBJECTIVE
  To investigate the effect of the anticoagulants on PRP quality, so as to clarify the appropriate anticoagulant used in PRP production.
  
  
  METHODS
  The microstructure change of platelets collected via heparin, citrate, acid citrate dextrose (ACD) and citrate-theophylline-adenosine-dipyridamole ( CTAD) was observed by TEM following time course. The extent of spontaneous activation of platelets in four groups was detected by measuring sP-selectin in plasma. The TGF-β1 release amount of activated PRP of four groups was measured.
  
  
  RESULTS
  CTAD is superior to other anticoagulants in maintaining the integrity of platelet structures for a long time and preventing platelet spontaneous activation. ACD slightly surpassed heparin and citrate in above two aspects. ACD-PRP and CTAD-PRP released significantly more TGF-β1 compared with heparin and citrate.
  
  
  CONCLUSIONS
  The PRP quality and biological effects were strongly associated with the type of Anticoagulants. ACD and CTAD are optimal anticoagulants in PRP production for they can maintain platelet viability at a high level.
authors.forename: Lei
authors.initials: L
authors.lastname: Hua
authors.forename: Gui
authors.initials: G
authors.lastname: Lai
authors.forename: Liu
authors.initials: L
authors.lastname: Zhenjun
authors.forename: Ma
authors.initials: M
authors.lastname: Guie
mesh_terms: D000241:Adenosine / Q000494:pharmacology; D000925:Anticoagulants / Q000494:pharmacology*; D001792:Blood Platelets / Q000187:drug effects* / Q000502:physiology; D002951:Citrates / Q000494:pharmacology; D019343:Citric Acid / Q000494:pharmacology; D004176:Dipyridamole / Q000494:pharmacology; D004338:Drug Combinations; D005947:Glucose / Q000031:analogs & derivatives / Q000494:pharmacology; D006493:Heparin / Q000494:pharmacology; D015539:Platelet Activation / Q000187:drug effects*; D053657:Platelet-Rich Plasma; D013806:Theophylline / Q000494:pharmacology; D053773:Transforming Growth Factor beta1 / Q000378:metabolism
chemical_list: D000925:Anticoagulants; D002951:Citrates; D004338:Drug Combinations; D053773:Transforming Growth Factor beta1; C002113:acid citrate dextrose; D019343:Citric Acid; D004176:Dipyridamole; D006493:Heparin; D013806:Theophylline; D005947:Glucose; D000241:Adenosine