title: Lentivirus‑mediated knockdown of MeCP2 inhibits the growth of colorectal cancer cells in vitro.
abstract: Methyl‑CpG‑binding protein 2 (MeCP2) is a transcriptional repressor that has been implicated in tumor onset and progression. Compared with normal and other tumorous tissue, MeCP2 is highly expressed in well‑differentiated adenocarcinoma and mucinous adenocarcinoma tissues, particularly at the invasion site of colorectal cancer tissues. The aim of the present study was to evaluate the potential of MeCP2 for use as a therapeutic target for human colorectal cancer. The DLD‑1 colorectal cancer cell line was subjected to lentivirus‑mediated short hairpin RNA‑induced knockdown of MeCP2 and the effects on cell growth, cell cycle progression and cell migration were assessed. It was confirmed that lentivirus‑mediated RNA interference successfully suppressed MeCP2 expression in vitro, which was demonstrated to result in reduced cell viability, cell cycle arrest in G0/G1 phase and inhibition of cell migration. These results indicated that MeCP2 may serve as a potential target for gene therapy of colorectal cancer.
authors.affiliation: General Surgery Department, The Affiliated Huashan Hospital of Fudan University, Shanghai 200040, P.R. China.
authors.forename: Ning
authors.initials: N
authors.lastname: Song
authors.affiliation: Oncomolecular Biology Laboratory of Ningbo No. 2 Hospital, Ningbo, Zhejiang 315010, P.R. China.
authors.forename: Keqiang
authors.initials: K
authors.lastname: Li
authors.affiliation: General Surgery Department, The Affiliated Huashan Hospital of Fudan University, Shanghai 200040, P.R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: General Surgery Department, The Affiliated Huashan Hospital of Fudan University, Shanghai 200040, P.R. China.
authors.forename: Zongyou
authors.initials: Z
authors.lastname: Chen
authors.affiliation: General Surgery Department, The Affiliated Huashan Hospital of Fudan University, Shanghai 200040, P.R. China.
authors.forename: Liubin
authors.initials: L
authors.lastname: Shi
mesh_terms: D059447:Cell Cycle Checkpoints; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D015179:Colorectal Neoplasms / Q000473:pathology*; D016193:G1 Phase; D055785:Gene Knockdown Techniques; D006801:Humans; D016086:Lentivirus / Q000378:metabolism*; D051783:Methyl-CpG-Binding Protein 2 / Q000378:metabolism*; D034622:RNA Interference; D016192:Resting Phase, Cell Cycle; D014410:Tumor Stem Cell Assay
chemical_list: D051783:Methyl-CpG-Binding Protein 2