title: In vivo evaluation of the biodistribution of intravenously administered naked and functionalised silver nanoparticles in rabbit.
abstract: Water-based suspension of silver nanoparticles (AgNPs) and dextran coated AgNPs (dextran-AgNPs) are fabricated and characterised for intravenous administration. A simple method for radiolabelling of nanoparticles with (99m)Tc was used. Labelling efficiency for AgNPs and dextran-AgNPs was found to be more than 80 and 88%, respectively. In vivo tissue uptake of nanoparticles during dynamic phase, after systematic administration by biodistribution analysis with single-photon emission computed tomography imaging has been evaluated. Biodistribution analysis revealed that (99m)Tc-AgNPs and (99m)Tc-dextran-AgNPs are mainly accumulated in liver/spleen region but (99m)Tc-dextran-AgNPs delayed recognition and uptake by liver. Results indicate that dextran-AgNPs are able to evade reticuloendothelum system with enhanced blood retention time. Accumulation of nanoparticles in liver/spleen region implicates the utilisation of AgNPs for liver cancer treatment.
authors.affiliation: Physics Department, University of Engineering and Technology, Lahore, Pakistan. ayesha.ashraf87@hotmail.com.
authors.forename: Ayesha
authors.initials: A
authors.lastname: Ashraf
authors.affiliation: Physics Department, University of Engineering and Technology, Lahore, Pakistan.
authors.forename: Rehana
authors.initials: R
authors.lastname: Sharif
authors.affiliation: Institute of Nuclear Medicine and Oncology (INMOL), Lahore, Pakistan.
authors.forename: Munir
authors.initials: M
authors.lastname: Ahmad
authors.affiliation: Institute of Nuclear Medicine and Oncology (INMOL), Lahore, Pakistan.
authors.forename: Misbah
authors.initials: M
authors.lastname: Masood
authors.affiliation: Institute of Nuclear Medicine and Oncology (INMOL), Lahore, Pakistan.
authors.forename: Abubakar
authors.initials: A
authors.lastname: Shahid
authors.affiliation: Advanced Nanofabrication Imaging and Characterization Laboratory, King Abdullah University of Science and Technology, Thuwal/Jeddah, Saudi Arabia.
authors.forename: Dalaver H
authors.initials: DH
authors.lastname: Anjum
authors.affiliation: Physics Department, University of Engineering and Technology, Lahore, Pakistan.
authors.forename: Muhammad Shahid
authors.initials: MS
authors.lastname: Rafique
authors.affiliation: Physics Department, University of Engineering and Technology, Lahore, Pakistan.
authors.forename: Sheeba
authors.initials: S
authors.lastname: Ghani
mesh_terms: D000818:Animals; D007275:Injections, Intravenous; D008099:Liver / Q000737:chemistry*; D008297:Male; D008422:Materials Testing; D053768:Metal Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry*; D009928:Organ Specificity; D010316:Particle Size; D011817:Rabbits; D012834:Silver / Q000008:administration & dosage* / Q000737:chemistry*; D013154:Spleen / Q000737:chemistry*; D014018:Tissue Distribution
chemical_list: D012834:Silver