title: Protective Effects of Salidroside on Mitochondrial Functions against Exertional Heat Stroke-Induced Organ Damage in the Rat.
abstract: Exertional heat stroke (EHS) results in a constellation of systemic inflammatory responses resulting in multiorgan failure and an extremely high mortality. The present study was designed to evaluate the protective effects of salidroside on EHS by improving mitochondrial functions in the rat model. Liver and heart mitochondria were observed by transmission electron microscopy and mitochondrial membrane potential (ΔΨm) was detected by a fluorescent probe. Intramitochondrial free Ca(2+) concentration, mitochondrial respiratory control ratio (RCR), reactive oxygen species (ROS) levels, superoxide dismutase (SOD), and malondialdehyde (MDA) activity were detected by the corresponding kits. RT-PCR was performed to estimate peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α) and manganese form of SOD (MnSOD) mRNA expression. The results demonstrated that salidroside was able to relieve EHS damage by reducing the swelling of mitochondria, ROS levels, and MDA activity, as well as increasing ΔΨm, RCR, free Ca(2+) concentration, SOD, PGC-1α, and MnSOD mRNA levels. In conclusion, salidroside has protective effects on mitochondrial functions against exertional heat stroke-induced organ damage in the rat.
authors.affiliation: Department of Emergency, The Second Military Medical University Affiliated Fuzhou General Hospital, Fuzhou, Fujian 350025, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Emergency, Fuzhou General Hospital, Fuzhou, Fujian 350025, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Peng
authors.affiliation: Department of Emergency, Fuzhou General Hospital, Fuzhou, Fujian 350025, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Emergency, Fuzhou General Hospital, Fuzhou, Fujian 350025, China.
authors.forename: Zhangwu
authors.initials: Z
authors.lastname: Xiao
authors.affiliation: Medical Department, Fuzhou General Hospital, Fuzhou 350025, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Song
authors.affiliation: Emergency Department, Shanghai Changzheng Hospital, Shanghai 200003, China.
authors.forename: Zhaofen
authors.initials: Z
authors.lastname: Lin