title: A nanohybrid of platinum nanoparticles-porous ZnO-hemin with electrocatalytic activity to construct an amplified immunosensor for detection of influenza.
abstract: In this work, a nanohybrid of platinum nanoparticles-porous ZnO spheres-hemin (Pt-pZnO-hemin) was synthesized for construction of alkaline phosphatase-based immunosensor for detection of influenza. Briefly, porous ZnO spheres (pZnO) were prepared using soluble starches as the capping agent, followed by surface functionalization of platinum nanoparticles via a hydrothermal method (Pt-pZnO). Then, hemin with carboxylic functionality was spontaneously adsorbed onto Pt-pZnO by ester-like binding between carboxylic group of hemin and ZnO. Compared with platinum nanoparticles and hemin, the resulting Pt-pZnO-hemin nanohybrid showed more excellent electrocatalysis activity toward 1-naphthol (1-NP). Taking advantage of the Pt-pZnO-hemin, we have developed an amplified electrochemical immunosensor based on in situ generation of redox probe by alkaline phosphatase (ALP) and Pt-pZnO-hemin as signal enhancer. Herein, electrochemically active 1-NP was generated by enzymatic hydrolysis of inactive 1-naphthyl phosphate by ALP, then Pt-pZnO-hemin was used as catalyst to catalytically oxidize 1-NP, resulting in electrochemical signal amplification. Furthermore, in comparison with other nanomaterials including Au-pZnO, Pt-pZnO and Au-pZnO-hemin, the excellent catalytical property of Pt-pZnO-hemin make it a promising nanohybrid material for ALP-based immunosensor for signal amplification.
authors.affiliation: Key Laboratory of Luminescence and Real-Time Analytic chemistry and Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
authors.forename: Zhe-Han
authors.initials: ZH
authors.lastname: Yang
authors.affiliation: Key Laboratory of Luminescence and Real-Time Analytic chemistry and Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhuo
authors.affiliation: Key Laboratory of Luminescence and Real-Time Analytic chemistry and Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China. Electronic address: ruoyuan@swu.edu.cn.
authors.forename: Ruo
authors.initials: R
authors.lastname: Yuan
authors.affiliation: Key Laboratory of Luminescence and Real-Time Analytic chemistry and Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China. Electronic address: yqchai@swu.edu.cn.
authors.forename: Ya-Qin
authors.initials: YQ
authors.lastname: Chai
mesh_terms: D000469:Alkaline Phosphatase / Q000737:chemistry; D015374:Biosensing Techniques; D055664:Electrochemical Techniques; D054856:G-Quadruplexes; D006427:Hemin / Q000737:chemistry; D006801:Humans; D007251:Influenza, Human / Q000175:diagnosis* / Q000821:virology; D053768:Metal Nanoparticles / Q000737:chemistry*; D009284:Naphthols / Q000737:chemistry / Q000302:isolation & purification*; D010984:Platinum / Q000737:chemistry; D015034:Zinc Oxide / Q000737:chemistry
chemical_list: D009284:Naphthols; C029350:1-naphthol; D010984:Platinum; D006427:Hemin; D000469:Alkaline Phosphatase; D015034:Zinc Oxide
keywords: Alkaline phosphatase; Nanohybrid; Platinum-porous ZnO spheres-hemin; Signal-amplified immunosensor