title: Effects of everolimus on a rat model of cerulein-induced experimental acute pancreatitis.
abstract: OBJECTIVE
  To analyze the biochemical and histopathological effects of everolimus in an experimental rat model of cerulein-induced acute pancreatitis. The aim of the present study was to determine the effects of everolimus on blood biochemical parameters and tissue histopathology in an experimental rat model of cerulein-induced acute pancreatitis.
  
  
  METHODS
  In 30 Wistar albino rats (male; 240-260 g), acute pancreatitis was induced by an intraperitoneal injection of cerulein (50 μg/kg) administered twice in 2 h. They were equally divided into the following three groups: 0.9% isotonic solution (Group 1; control), everolimus once (Group 2), and everolimus twice (Group 3) by oral gavage after cerulein injection. Thirty hours after the induction of pancreatitis, blood samples were collected by direct intracardiac puncture, rats were sacrificed, and pancreatic tissue samples were obtained.
  
  
  RESULTS
  Biochemical analyses of the blood samples showed statistically significant difference in red blood cell count as well as hemoglobin, hematocrit, urea, and alanine transaminase levels among the study groups (p<0.05 in all). Everolimus proved to significantly increase red blood cell count in a dose-independent manner. Hemoglobin and hematocrit levels significantly increased only after treatment with one dose of everolimus. Urea level was significantly different between the Groups 2 and 3; however, no change was observed in both groups when compared with the control. Alanine transaminase level significantly decreased only after treatment with two doses of everolimus. Histopathological analyses revealed that everolimus significantly decreased inflammation and perivascular infiltrate in a dose-dependent manner (35% in Group 2, 75% in Group 3; p=0.048).
  
  
  CONCLUSIONS
  Treatment with two doses of everolimus improved some biochemical and histopathological parameters of experimental rat models of cerulein-induced acute pancreatitis and implied the specific inhibition of inflammatory response pathways.
authors.affiliation: Clinic of General Surgery, Atatürk Training and Research Hospital, Ankara, Turkey.
authors.forename: Alper Bilal
authors.initials: AB
authors.lastname: Özkardeş
authors.affiliation: Clinic of General Surgery, Atatürk Training and Research Hospital, Ankara, Turkey.
authors.forename: Birkan
authors.initials: B
authors.lastname: Bozkurt
authors.affiliation: Clinic of General Surgery, Atatürk Training and Research Hospital, Ankara, Turkey.
authors.forename: Ersin Gürkan
authors.initials: EG
authors.lastname: Dumlu
authors.affiliation: Clinic of General Surgery, Atatürk Training and Research Hospital, Ankara, Turkey.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Tokaç
authors.affiliation: Clinic of Pathology, Atatürk Training and Research Hospital, Ankara, Turkey.
authors.forename: Aylin Kılıç
authors.initials: AK
authors.lastname: Yazgan
authors.affiliation: Department of Biochemistry, Yıldırım Beyazıt University Faculty of Medicine, Ankara, Turkey.
authors.forename: Merve
authors.initials: M
authors.lastname: Ergin
authors.affiliation: Department of Biochemistry, Yıldırım Beyazıt University Faculty of Medicine, Ankara, Turkey.
authors.forename: Özcan
authors.initials: Ö
authors.lastname: Erel
authors.affiliation: Department of General Surgery, Yıldırım Beyazıt University Faculty of Medicine, Ankara, Turkey.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Kılıç
keywords: Pancreatitis; cerulein; everolimus; inflammation