title: Dietary Mannoheptulose Does Not Significantly Alter Daily Energy Expenditure in Adult Labrador Retrievers.
abstract: Mannoheptulose (MH), a sugar found in avocados that inhibits glycolysis in vitro, has been preliminarily investigated as a novel food ingredient for dogs. This study aimed to determine the effects of dietary MH, delivered as an extract of un-ripened avocado, on energy expenditure (EE) in healthy adult Labrador Retriever dogs (total of 12 dogs, 26.99 ± 0.634 kg, 4.9 ± 0.2 y). The study was a double-blind, cross-over with each dog receiving both dietary treatments, control (CON) and MH (400 mg/kg of diet; 6 mg/kg BW), in random order. Resting and post-prandial (10 h) EE and respiratory quotient (RQ) were determined by indirect calorimetry (d 42). The following day, body composition was assessed using dual X-ray absorptiometry. Continuous activity monitoring was conducted using an Atical® accelerometer (d 43-47). A vastus lateralis muscle biopsy was obtained prior to the morning meal (d 49) and 4 h after consumption of their meal (d 56) to determine the protein content and phosphorylation of 5' adenosine monophosphate-activated protein kinase (AMPK). Diet did not affect body weight, resting EE or skeletal muscle AMPK phosphorylation. Dogs fed MH had significantly lower post-prandial RQ (p = 0.02) and ratio of fat to lean body mass (p = 0.02). Physical activity during light time periods (but not dark) was lower in dogs fed MH (p < 0.05) during weekends, but not on weekdays. These results suggest that MH affects energy balance of adult dogs, but that these effects are not dose dependent and not due to physical activity.
authors.affiliation: Centre for Nutrition Modelling, Department of Animal Biosciences, University of Guelph, Guelph, Ontario, N1G 2W1, Canada.
authors.forename: Leslie L
authors.initials: LL
authors.lastname: McKnight
authors.affiliation: Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, Ontario, N1G 2W1, Canada.
authors.forename: Jared
authors.initials: J
authors.lastname: Root-McCaig
authors.affiliation: Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, Ontario, N1G 2W1, Canada.
authors.forename: David
authors.initials: D
authors.lastname: Wright
authors.affiliation: Procter and Gamble Pet Care, 6571 State Route 503 North, Lewisburg, Ohio, 45338, United States of America.
authors.forename: Gary M
authors.initials: GM
authors.lastname: Davenport
authors.affiliation: Centre for Nutrition Modelling, Department of Animal Biosciences, University of Guelph, Guelph, Ontario, N1G 2W1, Canada.
authors.forename: James
authors.initials: J
authors.lastname: France
authors.affiliation: Centre for Nutrition Modelling, Department of Animal Biosciences, University of Guelph, Guelph, Ontario, N1G 2W1, Canada.|Procter and Gamble Pet Care, 6571 State Route 503 North, Lewisburg, Ohio, 45338, United States of America.
authors.forename: Anna Kate
authors.initials: AK
authors.lastname: Shoveller
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000378:metabolism; D000818:Animals; D001823:Body Composition / Q000187:drug effects; D001835:Body Weight / Q000187:drug effects; D018592:Cross-Over Studies; D004040:Dietary Carbohydrates / Q000008:administration & dosage* / Q000378:metabolism; D019587:Dietary Supplements; D004285:Dogs; D004311:Double-Blind Method; D004734:Energy Metabolism / Q000187:drug effects*; D005260:Female; D008297:Male; D008356:Mannoheptulose / Q000008:administration & dosage* / Q000378:metabolism; D009043:Motor Activity / Q000187:drug effects; D010084:Oxidation-Reduction; D010101:Oxygen Consumption / Q000187:drug effects
chemical_list: D004040:Dietary Carbohydrates; D008356:Mannoheptulose; D055372:AMP-Activated Protein Kinases