title: B Cells Loaded with Synthetic Particulate Antigens: A Versatile Platform To Generate Antigen-Specific Helper T Cells for Cell Therapy.
abstract: Adoptive cell therapy represents a promising approach for several chronic diseases. This study describes an innovative strategy for biofunctionalization of nanoparticles, allowing the generation of synthetic particulate antigens (SPAg). SPAg activate polyclonal B cells and vectorize noncognate proteins into their endosomes, generating highly efficient stimulators for ex vivo expansion of antigen-specific CD4+ T cells. This method also allows harnessing the ability of B cells to polarize CD4+ T cells into effectors or regulators.
authors.affiliation: International Center for Infectiology Research (CIRI); French National Institute of Health and Medical Research (INSERM) Unit 1111, Claude Bernard Lyon 1 University; Ecole Normale Supérieure de Lyon, CNRS, UMR 5308 , 69007 Lyon, France.|Edouard Herriot Hospital , Transplantation, Nephrology and Clinical Immunology Department, 69003 Lyon, France.
authors.forename: Antoine
authors.initials: A
authors.lastname: Sicard
authors.affiliation: International Center for Infectiology Research (CIRI); French National Institute of Health and Medical Research (INSERM) Unit 1111, Claude Bernard Lyon 1 University; Ecole Normale Supérieure de Lyon, CNRS, UMR 5308 , 69007 Lyon, France.|Edouard Herriot Hospital , Transplantation, Nephrology and Clinical Immunology Department, 69003 Lyon, France.
authors.forename: Alice
authors.initials: A
authors.lastname: Koenig
authors.affiliation: Sorbonne University , UPMC Univ Paris 06, INSERM U1135, CNRS ERL 8255, Center for Immunology and Microbial Infections - CIMI-Paris, F-75013, Paris, France.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Graff-Dubois
authors.affiliation: SFR Biosciences, UMS344/US8, Inserm, CNRS, Claude Bernard Lyon-1 University, Ecole Normale Supérieure , 69007 Lyon, France.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Dussurgey
authors.affiliation: Sorbonne University , UPMC Univ Paris 06, INSERM U1135, CNRS ERL 8255, Center for Immunology and Microbial Infections - CIMI-Paris, F-75013, Paris, France.
authors.forename: Angéline
authors.initials: A
authors.lastname: Rouers
authors.affiliation: French National Blood Service (EFS) , 69007 Lyon, France.
authors.forename: Valérie
authors.initials: V
authors.lastname: Dubois
authors.affiliation: International Center for Infectiology Research (CIRI); French National Institute of Health and Medical Research (INSERM) Unit 1111, Claude Bernard Lyon 1 University; Ecole Normale Supérieure de Lyon, CNRS, UMR 5308 , 69007 Lyon, France.
authors.forename: Pascal
authors.initials: P
authors.lastname: Blanc
authors.affiliation: International Center for Infectiology Research (CIRI); French National Institute of Health and Medical Research (INSERM) Unit 1111, Claude Bernard Lyon 1 University; Ecole Normale Supérieure de Lyon, CNRS, UMR 5308 , 69007 Lyon, France.
authors.forename: Dimitri
authors.initials: D
authors.lastname: Chartoire
authors.affiliation: Center for Quantitative imaging (CIQLE), SFR Santé Lyon-Est, Lyon 1 University , 69372 Lyon, France.
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Errazuriz-Cerda
authors.affiliation: International Center for Infectiology Research (CIRI); French National Institute of Health and Medical Research (INSERM) Unit 1111, Claude Bernard Lyon 1 University; Ecole Normale Supérieure de Lyon, CNRS, UMR 5308 , 69007 Lyon, France.
authors.forename: Helena
authors.initials: H
authors.lastname: Paidassi
authors.affiliation: International Center for Infectiology Research (CIRI); French National Institute of Health and Medical Research (INSERM) Unit 1111, Claude Bernard Lyon 1 University; Ecole Normale Supérieure de Lyon, CNRS, UMR 5308 , 69007 Lyon, France.
authors.forename: Morgan
authors.initials: M
authors.lastname: Taillardet
authors.affiliation: International Center for Infectiology Research (CIRI); French National Institute of Health and Medical Research (INSERM) Unit 1111, Claude Bernard Lyon 1 University; Ecole Normale Supérieure de Lyon, CNRS, UMR 5308 , 69007 Lyon, France.|Edouard Herriot Hospital , Transplantation, Nephrology and Clinical Immunology Department, 69003 Lyon, France.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Morelon
authors.affiliation: Sorbonne University , UPMC Univ Paris 06, INSERM U1135, CNRS ERL 8255, Center for Immunology and Microbial Infections - CIMI-Paris, F-75013, Paris, France.
authors.forename: Arnaud
authors.initials: A
authors.lastname: Moris
authors.affiliation: International Center for Infectiology Research (CIRI); French National Institute of Health and Medical Research (INSERM) Unit 1111, Claude Bernard Lyon 1 University; Ecole Normale Supérieure de Lyon, CNRS, UMR 5308 , 69007 Lyon, France.
authors.forename: Thierry
authors.initials: T
authors.lastname: Defrance
authors.affiliation: International Center for Infectiology Research (CIRI); French National Institute of Health and Medical Research (INSERM) Unit 1111, Claude Bernard Lyon 1 University; Ecole Normale Supérieure de Lyon, CNRS, UMR 5308 , 69007 Lyon, France.|Edouard Herriot Hospital , Transplantation, Nephrology and Clinical Immunology Department, 69003 Lyon, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Thaunat
mesh_terms: D001402:B-Lymphocytes / Q000276:immunology; D064987:Cell- and Tissue-Based Therapy; D006801:Humans; D016219:Immunotherapy, Adoptive; D008213:Lymphocyte Activation; D053758:Nanoparticles / Q000737:chemistry* / Q000627:therapeutic use; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology; D014614:Vaccines, Synthetic / Q000737:chemistry / Q000276:immunology* / Q000627:therapeutic use
chemical_list: D014614:Vaccines, Synthetic
keywords: B cell; CD4+ T cell; antigen-presenting cell; bioengineered nanoparticle; biomimetic; cell therapy