title: A STRIPAK component Strip regulates neuronal morphogenesis by affecting microtubule stability.
abstract: During neural development, regulation of microtubule stability is essential for proper morphogenesis of neurons. Recently, the striatin-interacting phosphatase and kinase (STRIPAK) complex was revealed to be involved in diverse cellular processes. However, there is little evidence that STRIPAK components regulate microtubule dynamics, especially in vivo. Here, we show that one of the core STRIPAK components, Strip, is required for microtubule organization during neuronal morphogenesis. Knockdown of Strip causes a decrease in the level of acetylated α-tubulin in Drosophila S2 cells, suggesting that Strip influences the stability of microtubules. We also found that Strip physically and genetically interacts with tubulin folding cofactor D (TBCD), an essential regulator of α- and β-tubulin heterodimers. Furthermore, we demonstrate the genetic interaction between strip and Down syndrome cell adhesion molecule (Dscam), a cell surface molecule that is known to work with TBCD. Thus, we propose that Strip regulates neuronal morphogenesis by affecting microtubule stability.
authors.affiliation: Department of Genetics, Graduate School of Pharmaceutical Sciences, The University of Tokyo.
authors.forename: Chisako
authors.initials: C
authors.lastname: Sakuma
authors.affiliation: Department of Genetics, Graduate School of Pharmaceutical Sciences, The University of Tokyo.
authors.forename: Misako
authors.initials: M
authors.lastname: Okumura
authors.affiliation: Department of Genetics, Graduate School of Pharmaceutical Sciences, The University of Tokyo.
authors.forename: Tomoki
authors.initials: T
authors.lastname: Umehara
authors.affiliation: Department of Genetics, Graduate School of Pharmaceutical Sciences, The University of Tokyo.|AMED-CREST, JST, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Miura
authors.affiliation: Department of Genetics, Graduate School of Pharmaceutical Sciences, The University of Tokyo.|AMED-CREST, JST, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Chihara
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D015815:Cell Adhesion Molecules / Q000378:metabolism; D029721:Drosophila Proteins / Q000378:metabolism; D055785:Gene Knockdown Techniques; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D008870:Microtubules / Q000378:metabolism*; D046912:Multiprotein Complexes / Q000378:metabolism*; D009474:Neurons / Q000166:cytology* / Q000378:metabolism*; D011485:Protein Binding; D055550:Protein Stability; D021381:Protein Transport; D014404:Tubulin / Q000378:metabolism
chemical_list: D002352:Carrier Proteins; D015815:Cell Adhesion Molecules; D029721:Drosophila Proteins; C491736:Dscam1 protein, Drosophila; D008869:Microtubule-Associated Proteins; D046912:Multiprotein Complexes; C000596801:TBCD protein, Drosophila; D014404:Tubulin