title: Blueberry (Vaccinium ashei Reade) extract ameliorates ovarian damage induced by subchronic cadmium exposure in mice: Potential δ-ALA-D involvement.
abstract: Females are born with a finite number of oocyte-containing follicles and ovary damage results in reduced fertility. Cadmium accumulates in the reproductive system, damaging it, and the cigarette smoke is a potential exposure route. Natural therapies are relevant to health benefits and disease prevention. This study verified the effect of cadmium exposure on the ovaries of mice and the blueberry extract as a potential therapy. Blueberry therapy was effective in restoring reactive species levels and δ-aminolevulinate dehydratase activity, and partially improved the viability of cadmium-disrupted follicles. This therapy was not able to restore the 17 β-hydroxysteroid dehydrogenase activity. Extract HPLC evaluation indicated the presence of quercetin, quercitrin, isoquercetin, and ascorbic acid. Ascorbic acid was the major substance and its concentration was 620.24 µg/mL. Thus, cadmium accumulates in the ovaries of mice after subchronic exposure, inducing cellular damage, and the blueberry extract possesses antioxidant properties that could protect, at least in part, the ovarian tissue from cadmium toxicity. © 2015 Wiley Periodicals, Inc. Environ Toxicol 32: 188-196, 2017.
authors.affiliation: Laboratório De Biotecnologia Da Reprodução (Biotech), Campus Uruguaiana, Universidade Federal Do Pampa, CEP 97500-970, Uruguaiana, Rio Grande do Sul, Brazil.
authors.forename: Aryele Pinto
authors.initials: AP
authors.lastname: Izaguirry
authors.affiliation: Laboratório De Biotecnologia Da Reprodução (Biotech), Campus Uruguaiana, Universidade Federal Do Pampa, CEP 97500-970, Uruguaiana, Rio Grande do Sul, Brazil.
authors.forename: Melina Bucco
authors.initials: MB
authors.lastname: Soares
authors.affiliation: Laboratório De Biotecnologia Da Reprodução (Biotech), Campus Uruguaiana, Universidade Federal Do Pampa, CEP 97500-970, Uruguaiana, Rio Grande do Sul, Brazil.
authors.forename: Laura Musacchio
authors.initials: LM
authors.lastname: Vargas
authors.affiliation: Laboratório De Biotecnologia Da Reprodução (Biotech), Campus Uruguaiana, Universidade Federal Do Pampa, CEP 97500-970, Uruguaiana, Rio Grande do Sul, Brazil.
authors.forename: Cristiano Chiapinotto
authors.initials: CC
authors.lastname: Spiazzi
authors.affiliation: Laboratório De Biotecnologia Da Reprodução (Biotech), Campus Uruguaiana, Universidade Federal Do Pampa, CEP 97500-970, Uruguaiana, Rio Grande do Sul, Brazil.
authors.forename: Daniela
authors.initials: D
authors.lastname: Dos Santos Brum
authors.affiliation: Laboratório De Biotecnologia Da Reprodução (Biotech), Campus Uruguaiana, Universidade Federal Do Pampa, CEP 97500-970, Uruguaiana, Rio Grande do Sul, Brazil.|Departamento De Química, Centro De Ciências Naturais E Exatas, Universidade Federal De Santa Maria, CEP 97105-900, Santa Maria, Rio Grande do Sul, Brazil.
authors.forename: Simone
authors.initials: S
authors.lastname: Noremberg
authors.affiliation: Laboratório De Desenvolvimento E Controle De Qualidade De Medicamentos (LDCQ), Campus Uruguaiana, Universidade Federal Do Pampa, CEP 97500-970, Uruguaiana, Rio Grande do Sul, Brazil.|Faculdade De Farmácia, Universidade Federal Do Rio Grande Do Sul, Av. Ipiranga 2752, CEP 90610-000, Porto Alegre, Rio Grande do Sul, Brazil.
authors.forename: Andreas Sebastian Loureiro
authors.initials: AS
authors.lastname: Mendez
authors.affiliation: Laboratório De Biotecnologia Da Reprodução (Biotech), Campus Uruguaiana, Universidade Federal Do Pampa, CEP 97500-970, Uruguaiana, Rio Grande do Sul, Brazil.
authors.forename: Francielli Weber
authors.initials: FW
authors.lastname: Santos
mesh_terms: D042842:11-beta-Hydroxysteroid Dehydrogenases / Q000378:metabolism; D000818:Animals; D029798:Blueberry Plants / Q000737:chemistry*; D002105:Cadmium Poisoning / Q000188:drug therapy* / Q000473:pathology; D005260:Female; D005979:Glutathione Peroxidase / Q000378:metabolism; D005981:Glutathione Synthase / Q000378:metabolism; D051379:Mice; D010049:Ovarian Diseases / Q000139:chemically induced* / Q000188:drug therapy* / Q000473:pathology; D006080:Ovarian Follicle / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D000623:Porphobilinogen Synthase / Q000187:drug effects / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D010936:Plant Extracts; D017382:Reactive Oxygen Species; D042842:11-beta-Hydroxysteroid Dehydrogenases; D005979:Glutathione Peroxidase; D000623:Porphobilinogen Synthase; D005981:Glutathione Synthase
keywords: blueberry; cadmium; follicles viability; ovary; δ-ALA-D