title: Photocleavable linker for the patterning of bioactive molecules.
abstract: Herein, we report the use of a versatile photocleavable nitrobenzyl linker to micropattern a wide variety of bioactive molecules and photorelease them on demand. On one end, the linker has an NHS group that can be coupled with any amine, such as peptides, proteins or amine-linkers, and on the other end an alkyne for convenient attachment to materials with an azide functional group. This linker was conjugated with NTA-amine or the cell adhesion peptide cRGD to enable straightforward patterning of His6-tagged proteins or cells, respectively, on PEGylated glass surfaces. This approach provides a practical way to control the presentation of a wide variety of bioactive molecules with high spatial and temporal resolution. The extent of photocleavage can also be controlled to tune the biomolecule density and degree of cell attachment to the surface.
authors.affiliation: Department of New Materials and Biosystems, Institution Max Planck Institute for Intelligent Systems, Heisenbergstr. 3, 70569 Stuttgart, Germany.|Department of Biophysical Chemistry, University of Heidelberg, Im Neuenheimer Feld 253, 69120 Heidelberg, Germany.
authors.forename: Seraphine V
authors.initials: SV
authors.lastname: Wegner
authors.affiliation: Department of New Materials and Biosystems, Institution Max Planck Institute for Intelligent Systems, Heisenbergstr. 3, 70569 Stuttgart, Germany.|Department of Biophysical Chemistry, University of Heidelberg, Im Neuenheimer Feld 253, 69120 Heidelberg, Germany.
authors.forename: Oya I
authors.initials: OI
authors.lastname: Sentürk
authors.affiliation: Department of New Materials and Biosystems, Institution Max Planck Institute for Intelligent Systems, Heisenbergstr. 3, 70569 Stuttgart, Germany.|Department of Biophysical Chemistry, University of Heidelberg, Im Neuenheimer Feld 253, 69120 Heidelberg, Germany.
authors.forename: Joachim P
authors.initials: JP
authors.lastname: Spatz
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D009842:Oligopeptides / Q000737:chemistry*; D055668:Photochemical Processes; D051381:Rats
chemical_list: D009842:Oligopeptides; C047981:arginyl-glycyl-aspartic acid