title: Apoferritin-templated biosynthesis of manganese nanoparticles and investigation of direct electron transfer of MnNPs-HsAFr at modified glassy carbon electrode.
abstract: Manganese nanoparticles (MnNPs) were created within horse spleen apoferritin (HsAFr) cavity nanotemplates. Transmission electron microscopy revealed the particle size to be 6 nm. Intrinsic fluorescence data showed that the mineralization acted as a quencher of the HsAFr fluorescence, and extrinsic fluorescence data revealed that the hydrophobic binding site at the surface of HsAFr was not changed. Finally, the MnNP-HsAFr was immobilized onto multiwalled carbon nanotubes entrapped into chitosan (CS) matrices by through sequential 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide-N-hydroxysuccinimide and glutaraldehyde coupling. The MnNPs-HsAFr immobilized on CNT-CS/GC electrode was characterized by cyclic voltammetry. This charge transfer coefficient (α) and the exchange current (i0) of MnNPs-HsAFr immobilized on modified electrode in 0.1 M phosphate solution (pH 7.5) were found to be 0.57 and 0.48 μA, respectively.
authors.affiliation: Department of Chemistry, College of Science, Kermanshah Branch, Islamic Azad University, Kermanshah, Iran.
authors.forename: Ronak
authors.initials: R
authors.lastname: Rafipour
authors.affiliation: Faculty of Chemistry, Sensor and Biosensor Research Center (SBRC) & Nanoscience and Nanotechnology Research Center (NNRC), Razi University, Kermanshah, Iran.|Nano Drug Delivery Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran.
authors.forename: Soheila
authors.initials: S
authors.lastname: Kashanian
authors.affiliation: Department of Animal Science, Faculty of Agriculture, University of Tehran, Karaj, Iran.
authors.forename: Sadegh
authors.initials: S
authors.lastname: Hashemi
authors.affiliation: Endocrine and Metabolism Research Center, Tehran University of Medical Sciences, Tehran, Iran.|Biosensor Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Kobra
authors.initials: K
authors.lastname: Omidfar
authors.affiliation: Research Center of Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Jafar
authors.initials: J
authors.lastname: Ezzati Nazhad Dolatabadi
mesh_terms: D001052:Apoferritins / Q000737:chemistry*; D004566:Electrodes; D004583:Electrons; D005898:Glass / Q000737:chemistry*; D006801:Humans; D008345:Manganese / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*
chemical_list: D008345:Manganese; D001052:Apoferritins
keywords: carbon nanotubes; chitosan; electron transfer; horse spleen apoferritin; manganese nanoparticles