title: Chlorophytum borivilianum Polysaccharide Fraction Provokes the Immune Function and Disease Resistance of Labeo rohita against Aeromonas hydrophila.
abstract: The present study aimed to investigate the effects of Chlorophytum borivilianum polysaccharide (CBP), as a dietary supplement administered at varying concentrations with feed (basal diet), on various cytokine-related responses in Labeo rohita fingerlings. Immune parameters and immune-related gene expressions were measured at 3rd, 4th, and 5th week after feeding. The results revealed that dietary administration of CBP at 0.2% and 0.4% for 4 weeks significantly upregulated serum lysozyme and phagocytic activity. Complement C3 and respiratory burst activity (RBA) were significantly higher after 4 weeks of CBP feeding. The immune related genes IL-8, IL-1β, TNF-α, and iNOS were downregulated (P < 0.05) in groups with 0.2% and 0.4% CBP supplemented diets at week 4. Expression of anti-inflammatory cytokines (IL-10 and TGF-β) was also downregulated (P < 0.5) after 4 weeks of feeding with 0.2% to 0.8% CBP. However, five weeks of CBP administration had no significant effect on immune gene expression, except TNF-α and IL-8. Fish fed with 0.4% CBP for 4 weeks showed maximum resistance against Aeromonas hydrophila (73.3% survival) compared to control. From these results, we recommend that CBP administration at 0.4% for 4 weeks could effectively improve immune response and disease resistance in L. rohita.
authors.affiliation: Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 151742, Republic of Korea.
authors.forename: Sib Sankar
authors.initials: SS
authors.lastname: Giri
authors.affiliation: School of Life Sciences, Jawaharlal Nehru University, New Delhi 110067, India.
authors.forename: Shib Sankar
authors.initials: SS
authors.lastname: Sen
authors.affiliation: Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 151742, Republic of Korea.
authors.forename: Cheng
authors.initials: C
authors.lastname: Chi
authors.affiliation: Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 151742, Republic of Korea.
authors.forename: Hyoun Joong
authors.initials: HJ
authors.lastname: Kim
authors.affiliation: Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 151742, Republic of Korea.
authors.forename: Saekil
authors.initials: S
authors.lastname: Yun
authors.affiliation: Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 151742, Republic of Korea.
authors.forename: Se Chang
authors.initials: SC
authors.lastname: Park
authors.affiliation: Department of Biotechnology, Periyar Maniammai University, Thanjavur, Tamil Nadu 613403, India.
authors.forename: V
authors.initials: V
authors.lastname: Sukumaran
mesh_terms: D016980:Aeromonas hydrophila / Q000276:immunology / Q000472:pathogenicity; D000821:Animal Feed; D000818:Animals; D003176:Complement C3 / Q000235:genetics; D003530:Cyprinidae; D019587:Dietary Supplements; D060467:Disease Resistance / Q000187:drug effects; D005393:Fish Diseases / Q000178:diet therapy* / Q000276:immunology / Q000382:microbiology / Q000401:mortality; D005786:Gene Expression Regulation; D016905:Gram-Negative Bacterial Infections / Q000178:diet therapy* / Q000276:immunology / Q000401:mortality / Q000662:veterinary*; D007113:Immunity, Innate / Q000187:drug effects*; D016753:Interleukin-10 / Q000235:genetics / Q000276:immunology; D053583:Interleukin-1beta / Q000235:genetics / Q000276:immunology; D016209:Interleukin-8 / Q000235:genetics / Q000276:immunology; D019668:Liliaceae / Q000737:chemistry; D009113:Muramidase / Q000235:genetics / Q000276:immunology; D052247:Nitric Oxide Synthase Type II / Q000235:genetics / Q000276:immunology; D010587:Phagocytosis / Q000187:drug effects; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D016897:Respiratory Burst / Q000187:drug effects; D016019:Survival Analysis; D016212:Transforming Growth Factor beta / Q000235:genetics / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000276:immunology
chemical_list: D003176:Complement C3; D053583:Interleukin-1beta; D016209:Interleukin-8; D011134:Polysaccharides; D016212:Transforming Growth Factor beta; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D052247:Nitric Oxide Synthase Type II; D009113:Muramidase