title: The protective effects of paeonol against epirubicin-induced hepatotoxicity in 4T1-tumor bearing mice via inhibition of the PI3K/Akt/NF-kB pathway.
abstract: Epirubicin is widely used for the treatment of various breast cancers; however, it has serious adverse side effects, such as hepatotoxicity, which require dose-adjustment or therapy substitution. Paeonol, an active component from Moutan Cortex, has a variety of biological activities, including preventing or reducing various toxicities induced by antineoplastics. Protection by paeonol against hepatotoxicity induced by epirubicin and the underlying mechanism of action were investigated in this study. Cytosolic enzymes in the serum and oxidative stress indices in the liver were determined. The protective effects were determined using the MTT assay in vitro or by evaluating the expression of apoptotic factors and crucial proteins in the PI3K/Akt/NF-kB pathway using western blot analysis. It is concluded that paeonol alleviates epirubicin-induced hepatotoxicity in 4T1-tumor bearing mice by inhibiting the PI3K/Akt/NF-kB pathway.
authors.affiliation: Department of Pharmacy, The Second Hospital of Shandong University, Jinan, China; Department of Pharmacology, School of Medicine, Shandong University, Jinan, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wu
authors.affiliation: Department of Pharmacy, The Second Hospital of Shandong University, Jinan, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Xue
authors.affiliation: Department of Pharmacy, The Second Hospital of Shandong University, Jinan, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Central Laboratory, The Second Hospital of Shandong University, Jinan, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Jiang
authors.affiliation: Department of Out-patient, People' Hospital of Zhangqiu, Jinan, China.
authors.forename: Hongmei
authors.initials: H
authors.lastname: Cao
authors.affiliation: Department of Pharmacy, The Second Hospital of Shandong University, Jinan, China.
authors.forename: Rongmei
authors.initials: R
authors.lastname: Wang
authors.affiliation: Department of Pharmacy, The Second Hospital of Shandong University, Jinan, China. Electronic address: sundq0405@126.com.
authors.forename: Deqing
authors.initials: D
authors.lastname: Sun
authors.affiliation: Institute of Clinical Pharmacology, Qi Lu Hospital of Shandong University, Jinan, China. Electronic address: grc7636@126.com.
authors.forename: Ruichen
authors.initials: R
authors.lastname: Guo
mesh_terms: D000098:Acetophenones / Q000494:pharmacology*; D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D056486:Chemical and Drug Induced Liver Injury / Q000150:complications / Q000188:drug therapy / Q000378:metabolism / Q000517:prevention & control*; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D015251:Epirubicin / Q000009:adverse effects*; D005260:Female; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008325:Mammary Neoplasms, Experimental / Q000150:complications / Q000188:drug therapy*; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects; D029596:Paeonia / Q000737:chemistry; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D020011:Protective Agents / Q000494:pharmacology; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects*; D013329:Structure-Activity Relationship
chemical_list: D000098:Acetophenones; D004365:Drugs, Chinese Herbal; D016328:NF-kappa B; D020011:Protective Agents; C038881:moutan cortex; C013638:paeonol; D015251:Epirubicin; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt
keywords: Breast cancer; Epirubicin; Hepatotoxicity; PI3K/Akt/NF-kB; Paeonol