title: Pseudotyping of lentiviral vector with novel vesiculovirus envelope glycoproteins derived from Chandipura and Piry viruses.
abstract: While the envelope glycoprotein of vesicular stomatitis virus (VSV-G) is widely used for pseudotyping of lentiviral vectors, sub-optimal gene transfer into certain cell types and its sensitivity to inactivation by human complement hinders its broader applications. To find alternative candidates, here we evaluated two serologically distinct novel viral envelopes derived from Chandipura (CNV-G) and Piry (PRV-G) vesiculoviruses. Both permitted generation of high titer psuedotyped lentiviral vectors with a capacity for high efficiency gene transfer into various cell types from different species. In human lymphoid and hematopoietic stem cells, their transduction efficiency was significantly lower than that of VSV-G. However, both novel envelopes were found to be more resistant to inactivation by human serum complement compared to VSV-G. Thus CNV-G and PRV-G envelopes can be harnessed for multiple uses in the future based on the cell type that needs to be gene transduced and possibly for in vivo gene transfer.
authors.affiliation: Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA.
authors.forename: Shuang
authors.initials: S
authors.lastname: Hu
authors.affiliation: Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA.
authors.forename: Dipu
authors.initials: D
authors.lastname: Mohan Kumar
authors.affiliation: Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA.
authors.forename: Chelsea
authors.initials: C
authors.lastname: Sax
authors.affiliation: Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA.
authors.forename: Clayton
authors.initials: C
authors.lastname: Schuler
authors.affiliation: Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA. Electronic address: akkina@colostate.edu.
authors.forename: Ramesh
authors.initials: R
authors.lastname: Akkina
mesh_terms: D061505:Cell Surface Display Techniques / Q000379:methods*; D002478:Cells, Cultured; D003165:Complement System Proteins; D018014:Gene Transfer Techniques; D006801:Humans; D016086:Lentivirus / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D013234:Stem Cells / Q000821:virology; D014161:Transduction, Genetic; D018116:Vesiculovirus / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D014759:Viral Envelope Proteins / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D038301:Virus Inactivation
chemical_list: D014759:Viral Envelope Proteins; D003165:Complement System Proteins
keywords: Chandipura and Piry viral glycoproteins; Gene transduction with pseudotyped lentiviral vectors; Human serum resistant lentiviral vectors; Lentiviral vector pseudotyping