title: Draxin from neocortical neurons controls the guidance of thalamocortical projections into the neocortex.
abstract: The thalamocortical tract carries sensory information to the neocortex. It has long been recognized that the neocortical pioneer axons of subplate neurons are essential for thalamocortical development. Herein we report that an axon guidance cue, draxin, is expressed in early-born neocortical neurons, including subplate neurons, and is necessary for thalamocortical development. In draxin(-/-) mice, thalamocortical axons do not enter the neocortex. This phenotype is sufficiently rescued by the transgenic expression of draxin in neocortical neurons. Genetic interaction data suggest that draxin acts through Deleted in colorectal cancer (DCC) and Neogenin (Neo1), to regulate thalamocortical projections in vivo. Draxin promotes the outgrowth of thalamic axons in vitro and this effect is abolished in thalamic neurons from Dcc and Neo1 double mutants. These results suggest that draxin from neocortical neurons controls thalamocortical projections into the neocortex, and that this effect is mediated through the DCC and Neo1 receptors.
authors.affiliation: Faculty of Life Sciences, Department of Developmental Neurobiology, Kumamoto University, Kumamoto 860-8556, Japan.|Department of Biophysical Genetics, Graduate School of Medical Sciences, Kanazawa University, Takara-machi 13-1, Ishikawa 920-8640, Japan.
authors.forename: Yohei
authors.initials: Y
authors.lastname: Shinmyo
authors.affiliation: Faculty of Life Sciences, Department of Developmental Neurobiology, Kumamoto University, Kumamoto 860-8556, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Asrafuzzaman Riyadh
authors.affiliation: Faculty of Life Sciences, Department of Developmental Neurobiology, Kumamoto University, Kumamoto 860-8556, Japan.
authors.forename: Giasuddin
authors.initials: G
authors.lastname: Ahmed
authors.affiliation: Faculty of Life Sciences, Department of Developmental Neurobiology, Kumamoto University, Kumamoto 860-8556, Japan.
authors.forename: Iftekhar
authors.initials: I
authors.lastname: Bin Naser
authors.affiliation: Faculty of Life Sciences, Department of Developmental Neurobiology, Kumamoto University, Kumamoto 860-8556, Japan.
authors.forename: Mahmud
authors.initials: M
authors.lastname: Hossain
authors.affiliation: Division of Neurobiology and Anatomy, Graduate School of Medical and Dental Sciences, Niigata University, Niigata 951-8510, Japan.
authors.forename: Hirohide
authors.initials: H
authors.lastname: Takebayashi
authors.affiliation: Department of Biophysical Genetics, Graduate School of Medical Sciences, Kanazawa University, Takara-machi 13-1, Ishikawa 920-8640, Japan.|Brain/Liver Interface Medicine Research Center, Kanazawa University, Ishikawa 920-8640, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kawasaki
authors.affiliation: Faculty of Life Sciences, Department of Developmental Neurobiology, Kumamoto University, Kumamoto 860-8556, Japan.
authors.forename: Kunimasa
authors.initials: K
authors.lastname: Ohta
authors.affiliation: Faculty of Life Sciences, Department of Developmental Neurobiology, Kumamoto University, Kumamoto 860-8556, Japan.
authors.forename: Hideaki
authors.initials: H
authors.lastname: Tanaka
mesh_terms: D000818:Animals; D001369:Axons / Q000502:physiology; D005260:Female; D057809:HEK293 Cells; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D008822:Mice, Transgenic; D019579:Neocortex / Q000166:cytology / Q000502:physiology*; D009474:Neurons / Q000502:physiology; D013788:Thalamus / Q000166:cytology / Q000502:physiology
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D008565:Membrane Proteins; C534831:draxin protein, mouse; C091279:neogenin