title: [Extraction of 10-Deacetyl Baccatin by Supercritical CO2 from Taxus yunnanensis Branches and Leaves].
abstract: OBJECTIVE
  To study the supercritical CO2 fluids extraction (SFE) method to extract the components from Taxus yunnanensis.
  
  
  METHODS
  Medicinal meterials were extracted by supercritical CO2, and then purified by industrial chromatography. Using the extraction yield of 10-DAB as the index,single factor test was carried out to investigate the effect of co-solvent, extraction time, extraction pressure, extraction temperature, pressure and temperature of separation kettle I. Then orthogonal experiment was used to optimize the best extraction condition.
  
  
  RESULTS
  The suitable extraction condition was as follows: the ratio of co-solvent (80% ethanol) amount and the madicinal materials was 3: 1, Separation kettle I pressure was 14 MPa, separation kettle I temperature was 40 °C, extraction pressure was 25 MPa, extraction temperature was 60 T and extraction time was 90 min. The extract was separated by industrial chromatographic and then crystallized.
  
  
  CONCLUSIONS
  The supercritical CO2 extraction and purification process of 10-DAB were simple and feasible.
authors.forename: Yang-qin
authors.initials: YQ
authors.lastname: Tang
authors.forename: Hai-chi
authors.initials: HC
authors.lastname: Li
authors.forename: Wen-jie
authors.initials: WJ
authors.lastname: Huang
authors.forename: Yan
authors.initials: Y
authors.lastname: Xiong
authors.forename: Fa-huan
authors.initials: FH
authors.lastname: Ge
mesh_terms: D025924:Chromatography, Supercritical Fluid; D000431:Ethanol; D018515:Plant Leaves / Q000737:chemistry*; D011312:Pressure; D012997:Solvents; D043823:Taxoids / Q000302:isolation & purification*; D020946:Taxus / Q000737:chemistry*; D013696:Temperature
chemical_list: D012997:Solvents; D043823:Taxoids; D000431:Ethanol; C042213:10-deacetylbaccatine III