title: Effect of cheY deletion on growth and colonization in a Haemophilus parasuis serovar 13 clinical strain EP3.
abstract: CheY, a response regulator of controlling the bacterial chemotactic swimming, can be modulated by either phosphorylation or acetylation to generate clockwise rotation of the flagella. Here, we researched the biological characteristics of cheY deletion mutant in Haemophilus parasuis, and found that the growth rate of this mutant was significantly slower compared with serovar 13 wild strain EP3. Additionally, the cheY mutant didn't show obvious sensitivity to porcine sera. The results of biofilm formation assay showed that H. parasuis cheY mutant produced less biofilm mass compared with wild strain. The H. parasuis cheY mutant reduced autoagglutination obviously. These findings were vital for revealing the function of cheY in growth, biofilm formation and autoagglutination. Thus, cheY plays a crucial role in growth and colonization in vivo of H. parasuis.
authors.affiliation: Research Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China. Electronic address: helvqin617@163.com.
authors.forename: Lvqin
authors.initials: L
authors.lastname: He
authors.affiliation: Research Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China. Electronic address: xintian3211@126.com.
authors.forename: Xintian
authors.initials: X
authors.lastname: Wen
authors.affiliation: Research Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China. Electronic address: yxuefeng251430@163.com.
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Yan
authors.affiliation: Research Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China. Electronic address: dlq318@126.com.
authors.forename: Lingqiang
authors.initials: L
authors.lastname: Ding
authors.affiliation: Research Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China. Electronic address: csanjie@sicau.edu.cn.
authors.forename: Sanjie
authors.initials: S
authors.lastname: Cao
authors.affiliation: Research Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China. Electronic address: rsghb110@126.com.
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Huang
authors.affiliation: Research Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China. Electronic address: wurui1977@163.com.
authors.forename: Rui
authors.initials: R
authors.lastname: Wu
authors.affiliation: Research Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China. Electronic address: yueliang5189@163.com.
authors.forename: Yiping
authors.initials: Y
authors.lastname: Wen
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D018441:Biofilms / Q000254:growth & development*; D002633:Chemotaxis / Q000235:genetics; D017353:Gene Deletion; D006192:Haemophilus Infections / Q000382:microbiology / Q000662:veterinary*; D044137:Haemophilus parasuis / Q000235:genetics* / Q000254:growth & development / Q000276:immunology; D010641:Phenotype; D017384:Sequence Deletion; D065288:Serogroup; D015610:Serum Bactericidal Test; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*
chemical_list: D001426:Bacterial Proteins
keywords: Autoagglutination; Biofilm formation; Haemophilus parasuis; Serum resistance; cheY