title: Infectious dengue vesicles derived from CD61+ cells in acute patient plasma exhibited a diaphanous appearance.
abstract: The levels of neutralizing antibody to a pathogen are an effective indicator to predict efficacy of a vaccine in trial. And yet not all the trial vaccines are in line with the theory. Using dengue virus (DENV) to investigate the viral morphology affecting the predictive value, we evaluated the viral morphology in acute dengue plasma compared to that of Vero cells derived DENV. The virions in plasma were infectious and heterogeneous in shape with a "sunny-side up egg" appearance, viral RNA was enclosed with CD61+ cell-derived membrane interspersed by the viral envelope protein, defined as dengue vesicles. The unique viral features were also observed from ex vivo infected human bone marrow. Dengue vesicles were less efficiently neutralized by convalescent patient serum, compared to virions produced from Vero cells. Our results exhibit a reason why potencies of protective immunity fail in vivo and significantly impact dengue vaccine and drug development.
authors.affiliation: Department of Microbiology and Immunology, Tainan, Taiwan.|Institute of Basic Medical Sciences, College of Medicine, Tainan, Taiwan.|Center of Infectious Disease and Signaling Research, Tainan, Taiwan.
authors.forename: Alan Yi-Hui
authors.initials: AY
authors.lastname: Hsu
authors.affiliation: Institute of Oral Medicine, National Cheng Kung University, Tainan, Taiwan.
authors.forename: Shang-Rung
authors.initials: SR
authors.lastname: Wu
authors.affiliation: Tropical Medicine Center, Kaohsiung, Taiwan.|Division of Infectious Diseases, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan.|Department of Internal Medicine, School of Medicine, Kaohsiung, Taiwan.|Center for Dengue Fever Control and Research, Kaohsiung Medical University, Kaohsiung, Taiwan.
authors.forename: Jih-Jin
authors.initials: JJ
authors.lastname: Tsai
authors.affiliation: Department of Internal Medicine, National Cheng Kung University, Tainan, Taiwan.
authors.forename: Po-Lin
authors.initials: PL
authors.lastname: Chen
authors.affiliation: Department of Internal Medicine, National Cheng Kung University, Tainan, Taiwan.
authors.forename: Ya-Ping
authors.initials: YP
authors.lastname: Chen
authors.affiliation: Department of Internal Medicine, National Cheng Kung University, Tainan, Taiwan.
authors.forename: Tsai-Yun
authors.initials: TY
authors.lastname: Chen
authors.affiliation: Center of Infectious Disease and Signaling Research, Tainan, Taiwan.|Institute of Bioinformatics and Biosignal Transduction, College of Bioscience and Biotechnology, National Cheng Kung University, Tainan, Taiwan.
authors.forename: Yu-Chih
authors.initials: YC
authors.lastname: Lo
authors.affiliation: Department of Microbiology and Immunology, Tainan, Taiwan.|Institute of Basic Medical Sciences, College of Medicine, Tainan, Taiwan.
authors.forename: Tzu-Chuan
authors.initials: TC
authors.lastname: Ho
authors.affiliation: Department of Microbiology and Immunology, Tainan, Taiwan.
authors.forename: Meed
authors.initials: M
authors.lastname: Lee
authors.affiliation: Institute of Oral Medicine, National Cheng Kung University, Tainan, Taiwan.
authors.forename: Min-Ting
authors.initials: MT
authors.lastname: Chen
authors.affiliation: Institute of Oral Medicine, National Cheng Kung University, Tainan, Taiwan.
authors.forename: Yen-Chi
authors.initials: YC
authors.lastname: Chiu
authors.affiliation: Department of Microbiology and Immunology, Tainan, Taiwan.|Institute of Basic Medical Sciences, College of Medicine, Tainan, Taiwan.|Center of Infectious Disease and Signaling Research, Tainan, Taiwan.
authors.forename: Guey Chuen
authors.initials: GC
authors.lastname: Perng
mesh_terms: D000818:Animals; D001692:Biological Transport; D001854:Bone Marrow Cells / Q000378:metabolism / Q000821:virology; D036022:Capsid Proteins / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D002522:Chlorocebus aethiops; D022162:Cytoplasmic Vesicles / Q000378:metabolism / Q000821:virology; D003715:Dengue / Q000276:immunology / Q000378:metabolism* / Q000821:virology*; D003716:Dengue Virus / Q000145:classification / Q000302:isolation & purification / Q000502:physiology* / Q000648:ultrastructure; D006801:Humans; D039661:Integrin beta3 / Q000378:metabolism*; D008533:Megakaryocytes / Q000378:metabolism / Q000821:virology; D010641:Phenotype; D065288:Serogroup; D014709:Vero Cells; D019562:Viral Load; D014771:Virion / Q000648:ultrastructure
chemical_list: D036022:Capsid Proteins; D039661:Integrin beta3