title: Prostaglandin E2 Regulation of Macrophage Innate Immunity.
abstract: Globally, maternal and fetal health is greatly impacted by extraplacental inflammation. Group B Streptococcus (GBS), a leading cause of chorioamnionitis, is thought to take advantage of the uterine environment during pregnancy in order to cause inflammation and infection. In this study, we demonstrate the metabolic changes of murine macrophages caused by GBS exposure. GBS alone prompted a delayed increase in lactate production, highlighting its ability to redirect macrophage metabolism from aerobic to anaerobic respiration. This production of lactate is thought to aid in the development and propagation of GBS throughout the surrounding tissue. Additionally, this study shows that PGE2 priming was able to exacerbate lactate production, shown by the rapid and substantial lactate increases seen upon GBS exposure. These data provide a novel model to study the role of GBS exposure to macrophages with and without PGE2 priming.
authors.forename: Danielle W
authors.initials: DW
authors.lastname: Kimmel
authors.affiliation: Department of Medicine, Division of Infectious Diseases, Vanderbilt University , Nashville, Tennessee 37232, United States.
authors.forename: Lisa M
authors.initials: LM
authors.lastname: Rogers
authors.affiliation: Department of Medicine, Division of Infectious Diseases, Vanderbilt University , Nashville, Tennessee 37232, United States.
authors.forename: David M
authors.initials: DM
authors.lastname: Aronoff
authors.forename: David E
authors.initials: DE
authors.lastname: Cliffel
mesh_terms: D000818:Animals; D002460:Cell Line; D015232:Dinoprostone / Q000276:immunology / Q000378:metabolism*; D007113:Immunity, Innate / Q000276:immunology*; D019344:Lactic Acid / Q000096:biosynthesis; D008070:Lipopolysaccharides / Q000276:immunology; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D013291:Streptococcus / Q000276:immunology
chemical_list: D008070:Lipopolysaccharides; D019344:Lactic Acid; D015232:Dinoprostone