title: Effects of Lactobacillus plantarum NCU116 on Intestine Mucosal Immunity in Immunosuppressed Mice.
abstract: The effects of Lactobacillus plantarum (L. plantarum) NCU116 isolated from pickled vegetables on intestine mucosal immunity in cyclophosphamide treated mice were investigated. Animals were divided into six groups: normal group (NIM), immunosuppression group (IM), immunosuppression plus L. plantarum NCU116 groups with three different doses (NCU-H, NCU-M, and NCU-L), and plus Bifidobacterium BB12 as positive control group (BB12). Results showed that the thymus indexes of the four treatment groups were significantly higher than that of the IM group (2.02 ± 0.16) (p < 0.05) and close to the index of the NIM group (2.61 ± 0.37) at 10 days. The level of immune factor IL-2 notably increased (IM, 121 ± 9.0) (p < 0.05) and was close to 65% of NIM group's level (230 ± 10.7). The levels of other immune factors (IFN-γ, IL-10, IL-12p70, and sIgA), the gene expression levels of IL-2 and IFN-γ, and the number of IgA-secreting cells showed similar patterns (p < 0.05). However, the level of immune factor IL-4 remarkably decreased (IM, 128 ± 10.2) (p < 0.05) and was only approximately 50% of the NIM group (154 ± 18.2). The levels of other immune factors (IL-6 and IgE) and the gene expression level of IL-6 at 10 days exhibited similar changes (p < 0.05) but showed a slight recovery at 20 days, accompanied by the altered protein expression levels of T-bet and GATA-3 in the small intestine. These findings suggest that L. plantarum NCU116 enhanced the immunity of the small intestine in the immunosuppressed mice.
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University , 235 Nanjing East Road, Nanchang 330047, China.
authors.forename: Junhua
authors.initials: J
authors.lastname: Xie
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University , 235 Nanjing East Road, Nanchang 330047, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University , 235 Nanjing East Road, Nanchang 330047, China.
authors.forename: Shaoping
authors.initials: S
authors.lastname: Nie
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University , 235 Nanjing East Road, Nanchang 330047, China.
authors.forename: Songtao
authors.initials: S
authors.lastname: Fan
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University , 235 Nanjing East Road, Nanchang 330047, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Xiong
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University , 235 Nanjing East Road, Nanchang 330047, China.
authors.forename: Mingyong
authors.initials: M
authors.lastname: Xie
mesh_terms: D000818:Animals; D002452:Cell Count; D003520:Cyclophosphamide / Q000008:administration & dosage; D016207:Cytokines / Q000032:analysis / Q000235:genetics; D005260:Female; D050990:GATA3 Transcription Factor / Q000032:analysis; D007070:Immunoglobulin A / Q000378:metabolism; D007165:Immunosuppression Therapy; D007413:Intestinal Mucosa / Q000276:immunology*; D007421:Intestine, Small / Q000737:chemistry / Q000166:cytology / Q000276:immunology; D048191:Lactiplantibacillus plantarum / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D019936:Probiotics; D012333:RNA, Messenger / Q000032:analysis; D013047:Specific Pathogen-Free Organisms; D020825:T-Box Domain Proteins / Q000032:analysis; D000098815:T-bet Transcription Factor
chemical_list: D016207:Cytokines; D050990:GATA3 Transcription Factor; C494748:Gata3 protein, mouse; D007070:Immunoglobulin A; D012333:RNA, Messenger; D020825:T-Box Domain Proteins; D000098815:T-bet Transcription Factor; D003520:Cyclophosphamide
keywords: Lactobacillus plantarum NCU116; Th1/Th2 balance; cyclophosphamide; immunomodulation; intestinal mucosal immune