title: Quantitative analysis of changes in the phosphoproteome of maize induced by the plant hormone salicylic acid.
abstract: Phytohormone salicylic acid (SA) plays an important role in regulating various physiological and biochemical processes. Our previous study identified several protein kinases responsive to SA, suggesting that phosphorylation events play an important role in the plant response to SA. In this study, we characterized the phosphoproteome of maize in response to SA using isotope tags for relative and absolute quantification (iTRAQ) technology and TiO2 enrichment method. Based on LC-MS/MS analysis, we found a total of 858 phosphoproteins among 1495 phosphopeptides. Among them, 291 phosphopeptides corresponding to 244 phosphoproteins were found to be significantly changed after SA treatment. The phosphoproteins identified are involved in a wide range of biological processes, which indicate that the response to SA encompasses a reformatting of major cellular processes. Furthermore, some of the phosphoproteins which were not previously known to be involved with SA were found to have significantly changed phosphorylation levels. Many of these changes are phosphorylation decreases, indicating that other currently unknown SA signaling pathways that result in decreased phosphorylation of downstream targets must be involved. Our study represents the first attempt at global phosphoproteome profiling in response to SA, and provides a better understanding of the molecular mechanisms regulated by SA.
authors.affiliation: Henan Agricultural University and Synergetic Innovation Center of Henan Grain Crops, Zhengzhou 450002, China.|Key Laboratory of Physiological Ecology and Genetic Improvement of Food Crops in Henan Province, Zhengzhou 450002, China.
authors.forename: Liuji
authors.initials: L
authors.lastname: Wu
authors.affiliation: Henan Agricultural University and Synergetic Innovation Center of Henan Grain Crops, Zhengzhou 450002, China.
authors.forename: Xiuli
authors.initials: X
authors.lastname: Hu
authors.affiliation: Henan Agricultural University and Synergetic Innovation Center of Henan Grain Crops, Zhengzhou 450002, China.|Key Laboratory of Physiological Ecology and Genetic Improvement of Food Crops in Henan Province, Zhengzhou 450002, China.
authors.forename: Shunxi
authors.initials: S
authors.lastname: Wang
authors.affiliation: Henan Agricultural University and Synergetic Innovation Center of Henan Grain Crops, Zhengzhou 450002, China.|Key Laboratory of Physiological Ecology and Genetic Improvement of Food Crops in Henan Province, Zhengzhou 450002, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Tian
authors.affiliation: North China University of Water Resources and Electric Power, Zhengzhou 450046, China.
authors.forename: Yanjie
authors.initials: Y
authors.lastname: Pang
authors.affiliation: College of Agronomy, Henan University of Science and Technology, Luoyang 471003, China.
authors.forename: Zanping
authors.initials: Z
authors.lastname: Han
authors.affiliation: Henan Agricultural University and Synergetic Innovation Center of Henan Grain Crops, Zhengzhou 450002, China.|Key Laboratory of Physiological Ecology and Genetic Improvement of Food Crops in Henan Province, Zhengzhou 450002, China.
authors.forename: Liancheng
authors.initials: L
authors.lastname: Wu
authors.affiliation: Henan Agricultural University and Synergetic Innovation Center of Henan Grain Crops, Zhengzhou 450002, China.|Key Laboratory of Physiological Ecology and Genetic Improvement of Food Crops in Henan Province, Zhengzhou 450002, China.
authors.forename: Yanhui
authors.initials: Y
authors.lastname: Chen
mesh_terms: D002853:Chromatography, Liquid; D010748:Phosphopeptides / Q000378:metabolism; D010750:Phosphoproteins / Q000378:metabolism*; D010766:Phosphorylation / Q000187:drug effects; D010937:Plant Growth Regulators / Q000494:pharmacology; D010940:Plant Proteins / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D020156:Salicylic Acid / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects; D053719:Tandem Mass Spectrometry; D003313:Zea mays / Q000187:drug effects* / Q000378:metabolism
chemical_list: D010748:Phosphopeptides; D010750:Phosphoproteins; D010937:Plant Growth Regulators; D010940:Plant Proteins; D020543:Proteome; D020156:Salicylic Acid