title: Microsatellite markers isolated from Cabomba aquatica s.l. (Cabombaceae) from an enriched genomic library.
abstract: OBJECTIVE
  Microsatellite primers were designed for the submersed aquatic plant Cabomba aquatica s.l. (Cabombaceae) and characterized to estimate genetic diversity parameters.
  
  
  RESULTS
  Using a selective hybridization method, we designed and tested 30 simple sequence repeat loci using two natural populations of C. aquatica s.l., resulting in 13 amplifiable loci. Twelve loci were polymorphic, and alleles per locus ranged from two to four across the 49 C. aquatica s.l. individuals. Observed heterozygosity, expected heterozygosity, and fixation index varied from 0.0 to 1.0, 0.0 to 0.5, and -1.0 to -0.0667, respectively, for the Manaus population and from 0.0 to 1.0, 0.0 to 0.6, and -1.0 to 0.4643 for the Viruá population.
  
  
  CONCLUSIONS
  The developed markers will be used in further taxonomic and population studies within Cabomba. This set of microsatellite primers represents the first report on rapid molecular markers in the genus.
authors.affiliation: Departamento de Biologia Vegetal, Instituto de Biologia, Caixa Postal 6109, Universidade Estadual de Campinas (UNICAMP), 13083-970 Campinas, São Paulo, Brazil.
authors.forename: Tiago D M
authors.initials: TD
authors.lastname: Barbosa
authors.affiliation: Departamento de Biologia Vegetal, Instituto de Biologia, Caixa Postal 6109, Universidade Estadual de Campinas (UNICAMP), 13083-970 Campinas, São Paulo, Brazil.
authors.forename: Rafaela J
authors.initials: RJ
authors.lastname: Trad
authors.affiliation: Departamento de Genética, Universidade de São Paulo (USP), Av. Pádua Dias 11, 13418-900 Piracicaba, São Paulo, Brazil.
authors.forename: Miklos M
authors.initials: MM
authors.lastname: Bajay
authors.affiliation: Departamento de Biologia Vegetal, Instituto de Biologia, Caixa Postal 6109, Universidade Estadual de Campinas (UNICAMP), 13083-970 Campinas, São Paulo, Brazil.
authors.forename: Maria C E
authors.initials: MC
authors.lastname: Amaral
keywords: Amazon; Cabomba aquatica s.l.; Cabombaceae; molecular markers; simple sequence repeat (SSR)