title: Autologous Platelet-Rich Plasma Preparations: Influence of Nonsteroidal Anti-inflammatory Drugs on Platelet Function.
abstract: BACKGROUND
  Autologous platelet-rich plasma (PRP) has been widely used for the treatment of sports injuries. It has been associated with improved healing and regeneration of soft tissues in elite athletes. Athletes are commonly receiving nonsteroidal anti-inflammatory drugs (NSAIDs). As yet, the effect of these drugs on platelet function in PRP formulations has not been taken into consideration.
  
  
  OBJECTIVE
  The function of platelets in PRP produced under the influence of NSAIDs is inhibited and may lessen a possible healing effect on the site of injury.
  
  
  METHODS
  Controlled laboratory study.
  
  
  METHODS
  PRP was collected from patients receiving NSAIDs after elective orthopaedic surgery, and platelet function was evaluated using light transmission aggregometry (LTA). Results were compared with those obtained from healthy volunteers without a history of NSAID intake during the previous 2 weeks. Two different systems for blood collection and PRP production (Arthrex ACP double-syringe system and standard 4.5-mL sodium citrate blood collection tubes) were used and compared regarding the quality of PRP that was produced.
  
  
  RESULTS
  For both groups, the baseline platelet counts of whole blood and the platelet counts of PRP formulations were found to be in the normal range. Both collection systems for PRP produced comparable results without significant differences between the groups. Platelet function testing with LTA revealed significantly impaired platelet aggregation in both PRP preparations, obtained from patients taking NSAIDs, irrespective of the type of NSAID (P < .001). All subjects from the control group showed normal platelet aggregation patterns when tested with LTA.
  
  
  CONCLUSIONS
  Autologous PRP produced from subjects after NSAID medication shows significantly impaired platelet function and may result in lower quality regarding the content of bioactive compounds.
  
  
  CONCLUSIONS
  If required, the administration of NSAIDs should be performed after blood collection for preparation of autologous PRP; otherwise, the therapeutic effect may be limited.
authors.affiliation: Sportchirurgieplus-Clinical Center for Sports Medicine and Orthopaedic Surgery, Graz, Austria.
authors.forename: Gert
authors.initials: G
authors.lastname: Schippinger
authors.affiliation: Clinical Institute of Medical and Chemical Laboratory Diagnostics, Medical University of Graz, Graz, Austria. ; Research Unit for Perioperative Platelet Function, Medical University of Graz, Graz, Austria.
authors.forename: Florian
authors.initials: F
authors.lastname: Prüller
authors.affiliation: Clinical Institute of Medical and Chemical Laboratory Diagnostics, Medical University of Graz, Graz, Austria. ; Research Unit for Perioperative Platelet Function, Medical University of Graz, Graz, Austria.
authors.forename: Manuela
authors.initials: M
authors.lastname: Divjak
authors.affiliation: Research Unit for Perioperative Platelet Function, Medical University of Graz, Graz, Austria. ; Department of Anesthesiology and Intensive Care Medicine, Medical University of Graz, Graz, Austria.
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Mahla
authors.affiliation: Sportchirurgieplus-Clinical Center for Sports Medicine and Orthopaedic Surgery, Graz, Austria.
authors.forename: Florian
authors.initials: F
authors.lastname: Fankhauser
authors.affiliation: Department of Health Sciences, Griffith University, Gold Coast, Queensland, Australia.
authors.forename: Steve
authors.initials: S
authors.lastname: Rackemann
authors.affiliation: Clinical Institute of Medical and Chemical Laboratory Diagnostics, Medical University of Graz, Graz, Austria. ; Research Unit for Perioperative Platelet Function, Medical University of Graz, Graz, Austria.
authors.forename: Reinhard Bernd
authors.initials: RB
authors.lastname: Raggam
keywords: nonsteroidal anti-inflammatory drugs; platelet function; platelet-rich plasma