title: Multimode fibre: Light-sheet microscopy at the tip of a needle.
abstract: Light-sheet fluorescence microscopy has emerged as a powerful platform for 3-D volumetric imaging in the life sciences. Here, we introduce an important step towards its use deep inside biological tissue. Our new technique, based on digital holography, enables delivery of the light-sheet through a multimode optical fibre--an optical element with extremely small footprint, yet permitting complex control of light transport processes within. We show that this approach supports some of the most advanced methods in light-sheet microscopy: by taking advantage of the cylindrical symmetry of the fibre, we facilitate the wavefront engineering methods for generation of both Bessel and structured Bessel beam plane illumination. Finally, we assess the quality of imaging on a sample of fluorescent beads fixed in agarose gel and we conclude with a proof-of-principle imaging of a biological sample, namely the regenerating operculum prongs of Spirobranchus lamarcki.
authors.affiliation: Ewing Building, School of Engineering, Physics &Mathematics, University of Dundee, Dundee, DD1 4HN, Scotland.|SUPA, School of Physics and Astronomy, University of St Andrews, St Andrews, Fife, KY16 9SS, Scotland.
authors.forename: Martin
authors.initials: M
authors.lastname: Plöschner
authors.affiliation: CEITEC - Central European Institute of Technology, Brno University of Technology, Technická 10, Brno 616 00, Czech Republic.
authors.forename: Věra
authors.initials: V
authors.lastname: Kollárová
authors.affiliation: CEITEC - Central European Institute of Technology, Brno University of Technology, Technická 10, Brno 616 00, Czech Republic.
authors.forename: Zbyněk
authors.initials: Z
authors.lastname: Dostál
authors.affiliation: SUPA, School of Physics and Astronomy, University of St Andrews, St Andrews, Fife, KY16 9SS, Scotland.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Nylk
authors.affiliation: The Scottish Oceans Institute, Gatty Marine Laboratory, University of St Andrews, St Andrews, Fife, KY16 8LB Scotland.
authors.forename: Thomas
authors.initials: T
authors.lastname: Barton-Owen
authors.affiliation: The Scottish Oceans Institute, Gatty Marine Laboratory, University of St Andrews, St Andrews, Fife, KY16 8LB Scotland.
authors.forename: David E K
authors.initials: DE
authors.lastname: Ferrier
authors.affiliation: CEITEC - Central European Institute of Technology, Brno University of Technology, Technická 10, Brno 616 00, Czech Republic.
authors.forename: Radim
authors.initials: R
authors.lastname: Chmelík
authors.affiliation: SUPA, School of Physics and Astronomy, University of St Andrews, St Andrews, Fife, KY16 9SS, Scotland.
authors.forename: Kishan
authors.initials: K
authors.lastname: Dholakia
authors.affiliation: Ewing Building, School of Engineering, Physics &Mathematics, University of Dundee, Dundee, DD1 4HN, Scotland.|School of Medicine, University of St Andrews, St Andrews, Fife, KY16 9TF Scotland.
authors.forename: Tomáš
authors.initials: T
authors.lastname: Čižmár
mesh_terms: D004867:Equipment Design / Q000379:methods; D021621:Imaging, Three-Dimensional / Q000379:methods; D008027:Light; D008029:Lighting / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods*; D009339:Needles; D055096:Optical Devices; D055100:Optical Fibers