title: Clinical relevance of thyroid cell models in redox research.
abstract: BACKGROUND
  Thyroid-derived cell models are commonly used to investigate the characteristics of thyroid cancers. It is noteworthy that each in vitro single cell model system imitates only a few characteristics of thyroid cancer depending on e.g. source of cells or oncogene used to transform the cells.
  
  
  METHODS
  In the current work we utilized rat thyroid cancer cell models to determine their clinical relevance in redox gene studies by comparing in vitro expression data to thyroid Oncomine microarray database. To survey the cell lines we analyzed mRNA expression of genes that produce superoxide anion (nox family), genes that catalyze destruction of superoxide anion to hydrogen peroxide (sod family), and genes that remove hydrogen peroxide from cellular environment (catalase, gpx family and prdx family).
  
  
  RESULTS
  Based on the current results, rat thyroid PC Cl3, PC PTC1, PC E1A, or FRLT5 cell models can be used to study NOX2, NOX4, SOD2, SOD3, CATALASE, GPX1, GPX2, GPX5, PRDX2, and PRDX3 gene expression and function.
  
  
  CONCLUSIONS
  Redox gene expression in rat originated single cell model systems used to study human thyroid carcinogenesis corresponds only partly with human redox gene expression, which may be caused by differences in redox gene activation stimulus. The data suggest careful estimation of the data observed in rat thyroid in vitro models.
authors.affiliation: IRCCS SDN, Via E. Gianturco 113, 80143 Naples, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Cammarota
authors.affiliation: IRCCS SDN, Via E. Gianturco 113, 80143 Naples, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Fiscardi
authors.affiliation: IRCCS SDN, Via E. Gianturco 113, 80143 Naples, Italy.
authors.forename: Tiziana
authors.initials: T
authors.lastname: Esposito
authors.affiliation: Department of Molecular Medicine and Medical Biotechnologies, University of Naples Federico II, 80014 Naples, Italy.
authors.forename: Gabriella
authors.initials: G
authors.lastname: de Vita
authors.affiliation: IRCCS SDN, Via E. Gianturco 113, 80143 Naples, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Salvatore
authors.affiliation: IRCCS SDN, Via E. Gianturco 113, 80143 Naples, Italy.
authors.forename: Mikko O
authors.initials: MO
authors.lastname: Laukkanen
keywords: Cell line; Cell model; Extracellular superoxide dismutase; FRTL5; PC Cl3; Rat; Redox gene expression; SOD3; Thyroid cancer