title: Retromer Is Essential for Autophagy-Dependent Plant Infection by the Rice Blast Fungus.
abstract: The retromer mediates protein trafficking through recycling cargo from endosomes to the trans-Golgi network in eukaryotes. However, the role of such trafficking events during pathogen-host interaction remains unclear. Here, we report that the cargo-recognition complex (MoVps35, MoVps26 and MoVps29) of the retromer is essential for appressorium-mediated host penetration by Magnaporthe oryzae, the causal pathogen of the blast disease in rice. Loss of retromer function blocked glycogen distribution and turnover of lipid bodies, delayed nuclear degeneration and reduced turgor during appressorial development. Cytological observation revealed dynamic MoVps35-GFP foci co-localized with autophagy-related protein RFP-MoAtg8 at the periphery of autolysosomes. Furthermore, RFP-MoAtg8 interacted with MoVps35-GFP in vivo, RFP-MoAtg8 was mislocalized to the vacuole and failed to recycle from the autolysosome in the absence of the retromer function, leading to impaired biogenesis of autophagosomes. We therefore conclude that retromer is essential for autophagy-dependent plant infection by the rice blast fungus.
authors.affiliation: Fujian-Taiwan Joint Center for Ecological Control of Crop Pests, College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.|Fujian University Key Laboratory for Functional Genomics of Plant Fungal Pathogens, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.
authors.forename: Wenhui
authors.initials: W
authors.lastname: Zheng
authors.affiliation: Fujian-Taiwan Joint Center for Ecological Control of Crop Pests, College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.|Fujian University Key Laboratory for Functional Genomics of Plant Fungal Pathogens, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Temasek Life Sciences Laboratory and Department of Biological Sciences, National University of Singapore, Singapore.
authors.forename: Yunlong
authors.initials: Y
authors.lastname: He
authors.affiliation: Fujian-Taiwan Joint Center for Ecological Control of Crop Pests, College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.|Fujian University Key Laboratory for Functional Genomics of Plant Fungal Pathogens, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.
authors.forename: Qiurong
authors.initials: Q
authors.lastname: Xie
authors.affiliation: Fujian-Taiwan Joint Center for Ecological Control of Crop Pests, College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.|Fujian University Key Laboratory for Functional Genomics of Plant Fungal Pathogens, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.
authors.forename: Ahai
authors.initials: A
authors.lastname: Chen
authors.affiliation: Fujian-Taiwan Joint Center for Ecological Control of Crop Pests, College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.|Fujian University Key Laboratory for Functional Genomics of Plant Fungal Pathogens, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.
authors.forename: Huawei
authors.initials: H
authors.lastname: Zheng
authors.affiliation: Fujian-Taiwan Joint Center for Ecological Control of Crop Pests, College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.|Fujian University Key Laboratory for Functional Genomics of Plant Fungal Pathogens, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Shi
authors.affiliation: Fujian-Taiwan Joint Center for Ecological Control of Crop Pests, College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.|Fujian University Key Laboratory for Functional Genomics of Plant Fungal Pathogens, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Fujian-Taiwan Joint Center for Ecological Control of Crop Pests, College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.|Fujian University Key Laboratory for Functional Genomics of Plant Fungal Pathogens, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.
authors.forename: Chengkang
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Fujian-Taiwan Joint Center for Ecological Control of Crop Pests, College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.|Fujian University Key Laboratory for Functional Genomics of Plant Fungal Pathogens, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.
authors.forename: Qingping
authors.initials: Q
authors.lastname: Huang
authors.affiliation: Fujian-Taiwan Joint Center for Ecological Control of Crop Pests, College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.|Fujian University Key Laboratory for Functional Genomics of Plant Fungal Pathogens, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.
authors.forename: Kunhai
authors.initials: K
authors.lastname: Fang
authors.affiliation: Fujian-Taiwan Joint Center for Ecological Control of Crop Pests, College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.|Fujian University Key Laboratory for Functional Genomics of Plant Fungal Pathogens, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.
authors.forename: Guodong
authors.initials: G
authors.lastname: Lu
authors.affiliation: Department of Plant Pathology and Microbiology, Texas A&M University, College Station, Texas, United States of America.
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Ebbole
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, United States of America.
authors.forename: Guangpu
authors.initials: G
authors.lastname: Li
authors.affiliation: Temasek Life Sciences Laboratory and Department of Biological Sciences, National University of Singapore, Singapore.
authors.forename: Naweed I
authors.initials: NI
authors.lastname: Naqvi
authors.affiliation: Fujian-Taiwan Joint Center for Ecological Control of Crop Pests, College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.|Fujian University Key Laboratory for Functional Genomics of Plant Fungal Pathogens, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.
authors.forename: Zonghua
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D001343:Autophagy / Q000235:genetics; D006003:Glycogen / Q000378:metabolism; D054884:Host-Pathogen Interactions / Q000235:genetics; D066292:Lipid Droplets / Q000378:metabolism; D020082:Magnaporthe / Q000235:genetics* / Q000472:pathogenicity; D012275:Oryza / Q000235:genetics* / Q000382:microbiology; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism / Q000382:microbiology; D021381:Protein Transport / Q000235:genetics*; D014617:Vacuoles / Q000235:genetics / Q000382:microbiology; D021601:trans-Golgi Network / Q000235:genetics
chemical_list: D006003:Glycogen