title: Prohibitin as the Molecular Binding Switch in the Retinal Pigment Epithelium.
abstract: Previously, our molecular binding study showed that prohibitin interacts with phospholipids, including phosphatidylinositide and cardiolipin. Under stress conditions, prohibitin interacts with cardiolipin as a retrograde response to activate mitochondrial proliferation. The lipid-binding switch mechanism of prohibitin with phosphatidylinositol-3,4,5-triphosphate and cardiolipin may suggest the role of prohibitin effects on energy metabolism and age-related diseases. The current study examined the region-specific expressions of prohibitin with respect to the retina and retinal pigment epithelium (RPE) in age-related macular degeneration (AMD). A detailed understanding of prohibitin binding with lipids, nucleotides, and proteins shown in the current study may suggest how molecular interactions control apoptosis and how we can intervene against the apoptotic pathway in AMD. Our data imply that decreased prohibitin in the peripheral RPE is a significant step leading to mitochondrial dysfunction that may promote AMD progression.
authors.affiliation: Department of Biological Sciences, Michigan Technological University, Houghton, MI, 49931, USA.|Department of Ophthalmology, Wilmer Eye Institute, The Johns Hopkins University School of Medicine, Baltimore, MD, 21287, USA.
authors.forename: Srinivas R
authors.initials: SR
authors.lastname: Sripathi
authors.affiliation: Retina Proteomics Laboratory, Department of Petroleum Chemistry, American University of Nigeria, Yola, Nigeria.
authors.forename: O'Donnell
authors.initials: O
authors.lastname: Sylvester
authors.affiliation: Department of Biological Sciences, Michigan Technological University, Houghton, MI, 49931, USA.
authors.forename: Weilue
authors.initials: W
authors.lastname: He
authors.affiliation: Department of Biological Sciences, Michigan Technological University, Houghton, MI, 49931, USA.
authors.forename: Trevor
authors.initials: T
authors.lastname: Moser
authors.affiliation: Department of Biological Sciences, Michigan Technological University, Houghton, MI, 49931, USA.
authors.forename: Ji-Yeon
authors.initials: JY
authors.lastname: Um
authors.affiliation: Department of Ophthalmology, Georgia Regents University, Augusta, GA, 30912, USA.
authors.forename: Folami
authors.initials: F
authors.lastname: Lamoke
authors.affiliation: Department of Biological Sciences, Michigan Technological University, Houghton, MI, 49931, USA.
authors.forename: Wusirika
authors.initials: W
authors.lastname: Ramakrishna
authors.affiliation: Department of Ophthalmology and Visual Sciences, Moran Eye Center, University of Utah School of Medicine, Salt Lake City, UT, 84132, USA.
authors.forename: Paul S
authors.initials: PS
authors.lastname: Bernstein
authors.affiliation: Department of Ophthalmology, Georgia Regents University, Augusta, GA, 30912, USA.
authors.forename: Manuela
authors.initials: M
authors.lastname: Bartoli
authors.affiliation: Retina Proteomics Laboratory, Department of Petroleum Chemistry, American University of Nigeria, Yola, Nigeria. wan.jahng@aun.edu.ng.
authors.forename: Wan Jin
authors.initials: WJ
authors.lastname: Jahng
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D006801:Humans; D050356:Lipid Metabolism; D008055:Lipids / Q000737:chemistry; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008958:Models, Molecular; D008969:Molecular Sequence Data; D018384:Oxidative Stress; D000091402:Prohibitins; D051381:Rats; D017207:Rats, Sprague-Dawley; D012097:Repressor Proteins / Q000737:chemistry* / Q000378:metabolism*; D055213:Retinal Pigment Epithelium / Q000166:cytology* / Q000378:metabolism*; D016415:Sequence Alignment
chemical_list: D008055:Lipids; D000091402:Prohibitins; D012097:Repressor Proteins
keywords: Macular degeneration; Mitochondria; Oxidative stress; Prohibitin; Retinal pigment epithelium