title: Fluorescent Labeling of Plasmid DNA and mRNA: Gains and Losses of Current Labeling Strategies.
abstract: Live-cell imaging has provided the life sciences with insights into the cell biology and dynamics. Fluorescent labeling of target molecules proves to be indispensable in this regard. In this Review, we focus on the current fluorescent labeling strategies for nucleic acids, and in particular mRNA (mRNA) and plasmid DNA (pDNA), which are of interest to a broad range of scientific fields. By giving a background of the available techniques and an evaluation of the pros and cons, we try to supply scientists with all the information needed to come to an informed choice of nucleic acid labeling strategy aimed at their particular needs.
authors.affiliation: Laboratory of general biochemistry and physical pharmacy, Faculty of pharmacy and ‡Centre for Nano- and Biophotonics, Ghent University , Ghent 9000, Belgium.
authors.forename: K
authors.initials: K
authors.lastname: Rombouts
authors.affiliation: Laboratory of general biochemistry and physical pharmacy, Faculty of pharmacy and ‡Centre for Nano- and Biophotonics, Ghent University , Ghent 9000, Belgium.
authors.forename: K
authors.initials: K
authors.lastname: Braeckmans
authors.affiliation: Laboratory of general biochemistry and physical pharmacy, Faculty of pharmacy and ‡Centre for Nano- and Biophotonics, Ghent University , Ghent 9000, Belgium.
authors.forename: K
authors.initials: K
authors.lastname: Remaut
mesh_terms: D004247:DNA / Q000032:analysis*; D005456:Fluorescent Dyes / Q000737:chemistry*; D061848:Optical Imaging / Q000379:methods*; D010957:Plasmids / Q000737:chemistry*; D011119:Polynucleotides / Q000737:chemistry; D012333:RNA, Messenger / Q000032:analysis*; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes; D011119:Polynucleotides; D012333:RNA, Messenger; D004247:DNA