title: Validation of the leukocyte labeling procedure with a new kit for the preparation of technetium-99m exametazime injection.
abstract: The labeling of leukocytes with technetium-99m (Tc)-HMPAO is a frequent procedure in our laboratory, which was first validated before initiation of clinical studies with the commercial kit of Tc-D,L-hexamethylpropylene amine oxime (Tc-D,L-HMPAO), available from 1988. Recently, a new kit of exametazime has been introduced, with a similar composition and clinical indications, but with some technical improvements. Validation of the labeling procedure with this new kit is proposed, including a comparison between both commercial kits. Requirements for validation were achieved successfully and a comparative study showed no statistically significant differences; nevertheless, labeling of leukocytes may be expected routinely with the new kit on account of its longer shelf-life and lower cost.
authors.affiliation: aRadiopharmacy Unit bNuclear Medicine Department, Hospital General Universitario Santa Lucia, Cartagena, Murcia, Spain.
authors.forename: Teresa
authors.initials: T
authors.lastname: Martinez
authors.forename: Marta
authors.initials: M
authors.lastname: Gallego Peinado
authors.forename: Jose
authors.initials: J
authors.lastname: Sanchez Catalicio
authors.forename: Florentina
authors.initials: F
authors.lastname: Perez Angel
authors.forename: Jose
authors.initials: J
authors.lastname: Contreras Gutiérrez
mesh_terms: D002470:Cell Survival; D007267:Injections; D007553:Isotope Labeling / Q000379:methods*; D007962:Leukocytes / Q000166:cytology / Q000378:metabolism*; D008168:Lung / Q000166:cytology; D011786:Quality Control; D011854:Radiochemistry; D019690:Technetium Tc 99m Exametazime / Q000008:administration & dosage / Q000378:metabolism*
chemical_list: D019690:Technetium Tc 99m Exametazime