title: Rabies virus phosphoprotein interacts with ribosomal protein L9 and affects rabies virus replication.
abstract: Rabies virus is a highly neurotropic virus that can cause fatal infection of the central nervous system in warm-blooded animals. The RABV phosphoprotein (P), an essential cofactor of the virus RNA-dependent RNA polymerase, is required for virus replication. In this study, the ribosomal protein L9, which has functions in protein translation, is identified as P-interacting cellular factor using phage display analysis. Direct binding between the L9 and P was confirmed by protein pull-down and co-immunoprecipitation analyses. It was further demonstrated that L9 translocates from the nucleus to the cytoplasm, where it colocalizes with P in cells infected with RABV or transfected with P gene. RABV replication was reduced with L9 overexpression and enhanced with L9 knockdown. Thus, we propose that during RABV infection, P binds to L9 that translocates from the nucleus to the cytoplasm, inhibiting the initial stage of RABV transcription.
authors.affiliation: The National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei 430070, China; College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei 430070, China; College of Animal Science, Tarim University, Alar, Xinjiang, China.
authors.forename: Youwen
authors.initials: Y
authors.lastname: Li
authors.affiliation: The National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei 430070, China; College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
authors.forename: Wanyu
authors.initials: W
authors.lastname: Dong
authors.affiliation: The National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei 430070, China; College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
authors.forename: Yuejun
authors.initials: Y
authors.lastname: Shi
authors.affiliation: The National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei 430070, China; College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Deng
authors.affiliation: The National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei 430070, China; College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.affiliation: College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
authors.forename: Chunyun
authors.initials: C
authors.lastname: Wan
authors.affiliation: The National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei 430070, China; College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Zhou
authors.affiliation: The National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei 430070, China; College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Zhao
authors.affiliation: The National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei 430070, China; College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei 430070, China; Departments of Pathology, College of Veterinary Medicine, University of Georgia, Athens, GA 30602, USA. Electronic address: zhenfu@uga.edu.
authors.forename: Zhen F
authors.initials: ZF
authors.lastname: Fu
authors.affiliation: The National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei 430070, China; College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei 430070, China. Electronic address: penggq@mail.hzau.edu.cn.
authors.forename: Guiqing
authors.initials: G
authors.lastname: Peng
mesh_terms: D002460:Cell Line; D061505:Cell Surface Display Techniques; D002498:Centrifugation; D006801:Humans; D047468:Immunoprecipitation; D018832:Molecular Chaperones; D010750:Phosphoproteins / Q000378:metabolism*; D011485:Protein Binding; D025941:Protein Interaction Mapping; D011820:Rabies virus / Q000502:physiology*; D012269:Ribosomal Proteins / Q000378:metabolism*; D015678:Viral Structural Proteins / Q000378:metabolism*; D014779:Virus Replication
chemical_list: D018832:Molecular Chaperones; C103961:P phosphoprotein, Rabies virus; D010750:Phosphoproteins; D012269:Ribosomal Proteins; D015678:Viral Structural Proteins; C049650:ribosomal protein L9
keywords: Protein interaction; Rabies virus; Ribosomal protein L9; Viral replication and transcription