title: Biocompatible, Free-Standing Film Composed of Bacterial Cellulose Nanofibers-Graphene Composite.
abstract: In recent years, graphene films have been used in a series of wide applications in the biomedical area, because of several advantageous characteristics. Currently, these films are derived from graphene oxide (GO) via chemical or physical reduction methods, which results in a significant decrease in surface hydrophilicity, although the electrical property could be greatly improved, because of the reduction process. Hence, the comprehensive performance of the graphene films showed practical limitations in the biomedical field, because of incompatibility of highly hydrophobic surfaces to support cell adhesion and growth. In this work, we present a novel fabrication of bacterial cellulose nanofibers/reduced graphene oxide (BC-RGO) film, using a bacterial reduction method. Thus-prepared BC-RGO films maintained excellent hydrophilicity, while electrical properties were improved by bacterial reduction of GO films in culture. Human marrow mesenchymal stem cells (hMSCs) cultured on these surfaces showed improved cellular response with higher cell proliferation on the BC-RGO film, compared to free-standing reduced graphene oxide film without the nanoscale fibrous structure. Furthermore, the cellular adhesion and proliferation were even comparable to that on the tissue culture plate, indicating that the bacterial cellulose nanofibers play a critically contructive role in supporting cellular activities. The novel fabrication method greatly enhanced the biochemical activity of the cells on the surface, which could aid in realizing several potential applications of graphene film in biomedical area, such as tissue engineering, bacterial devices, etc.
authors.affiliation: The Key Laboratory of Rare Earth Functional Materials and Applications, Zhoukou Normal University , Zhoukou 466001, People's Republic of China.|Materials Science Institute, PCFM Lab and DSAPM Lab, School of Chemistry and Chemical Engineering, Sun Yat-sen University , Guangzhou 510275, People's Republic of China.|School of Mechanical & Aerospace Engineering, Nanyang Technological University , 50 Nanyang Avenue, 639798 Singapore.
authors.forename: Lin
authors.initials: L
authors.lastname: Jin
authors.affiliation: School of Chemical & Biological Engineering, Nanyang Technological University , 50 Nanyang Avenue, 639798 Singapore.
authors.forename: Zhiping
authors.initials: Z
authors.lastname: Zeng
authors.affiliation: School of Mechanical & Aerospace Engineering, Nanyang Technological University , 50 Nanyang Avenue, 639798 Singapore.
authors.forename: Shreyas
authors.initials: S
authors.lastname: Kuddannaya
authors.affiliation: Materials Science Institute, PCFM Lab and DSAPM Lab, School of Chemistry and Chemical Engineering, Sun Yat-sen University , Guangzhou 510275, People's Republic of China.
authors.forename: Dingcai
authors.initials: D
authors.lastname: Wu
authors.affiliation: School of Mechanical & Aerospace Engineering, Nanyang Technological University , 50 Nanyang Avenue, 639798 Singapore.
authors.forename: Yilei
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: The Key Laboratory of Rare Earth Functional Materials and Applications, Zhoukou Normal University , Zhoukou 466001, People's Republic of China.
authors.forename: Zhenling
authors.initials: Z
authors.lastname: Wang
mesh_terms: D001672:Biocompatible Materials / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002482:Cellulose / Q000494:pharmacology*; D005453:Fluorescence; D006108:Graphite / Q000494:pharmacology*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D010084:Oxidation-Reduction; D013059:Spectrum Analysis, Raman; D013314:Stress, Mechanical
chemical_list: D001672:Biocompatible Materials; D006108:Graphite; D002482:Cellulose
keywords: bacterial reduction; cellulose nanofibers; graphene; hMSCs; tissue engineering