title: Rapid Enantiomeric Separation and Quantitation of Levetiracetam on α-Acid Glycoprotein (AGP) Chiral Stationary Phase by High-Performance Liquid Chromatography.
abstract: A new, simple, and rapid chiral HPLC method was developed for enantioselective analysis of levetiracetam and its enantiomer [(R)-α-ethyl-2- oxo-pyrrolidine acetamide] in a pharmaceutical formulation and bulk material. Enantiomeric separation was achieved on a chiral-α1-acid glycoprotein (AGP) column (150×4.0 mm, 5 μm) using an isocratic mobile phase of phosphate buffer (pH=7) at a flow rate of 0.7 mL/min. The UV detector was set at 210 nm. Calibration curves were linear in the range of 1-100 μg/mL and 0.4-20 μg/mL for levetiracetam and the (R)-enantiomer, respectively. LOD and LOQ for the (R)-enantiomer were 0.1 and 0.4 μg/mL, respectively. The run time of analysis was less than 5.0 min.
authors.affiliation: Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, PO Box 68149-89468, Khorramabad, Iran.
authors.forename: Rouhollah
authors.initials: R
authors.lastname: Heydari
authors.forename: Mojtaba
authors.initials: M
authors.lastname: Shamsipur
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D000077287:Levetiracetam; D057230:Limit of Detection; D009961:Orosomucoid / Q000737:chemistry*; D010889:Piracetam / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry; D013237:Stereoisomerism
chemical_list: D009961:Orosomucoid; D000077287:Levetiracetam; D010889:Piracetam