title: Variable number of tandem repeat profiles and antimicrobial resistance patterns of Staphylococcus haemolyticus strains isolated from blood cultures in children.
abstract: Staphylococcus haemolyticus is a healthcare-associated pathogen and can cause a variety of lifethreatening infections. Additionally, multi-drug resistance (MDR), in particular methicillin-resistant S. haemolyticus (MRSH) isolates, have emerged. Dissemination of such strains can be of great concern in the hospital environment. A total number of 20S. haemolyticus isolates from blood cultures obtained from children were included in this study. A high prevalence of MDR-MRSH isolates with high MIC values to vancomycin was found and 35% of the isolates were intermediate resistant to vancomycin. Multilocus variable number of tandem repeats analysis (MLVF) revealed 5 MLVF types among 20 isolates of S. haemolyticus. Twelve isolates shared the same MLVF type and were isolated from different wards in a pediatric hospital in Iran. This is a serious alarm for infection control; i.e. in the absence of adequate infection diagnostics and infection control guidelines, these resistant strains can spread to other sectors of a hospital and possibly among the community.
authors.affiliation: Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Faride
authors.initials: F
authors.lastname: Hosseinkhani
authors.affiliation: Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Fereshteh
authors.initials: F
authors.lastname: Jabalameli
authors.affiliation: Children's Medical Center, Tehran University of Medical Science, Tehran, Iran.
authors.forename: Narges
authors.initials: N
authors.lastname: Nodeh Farahani
authors.affiliation: Department of Microbiology, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran.
authors.forename: Morovat
authors.initials: M
authors.lastname: Taherikalani
authors.affiliation: Department of Innovative Molecular Diagnostics, University of Applied Sciences Leiden, Leiden, The Netherlands.
authors.forename: Willem B
authors.initials: WB
authors.lastname: van Leeuwen
authors.affiliation: Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. Electronic address: emaneini@tums.ac.ir.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Emaneini
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D016470:Bacteremia / Q000382:microbiology*; D002675:Child, Preschool; D004269:DNA, Bacterial; D024881:Drug Resistance, Bacterial; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008826:Microbial Sensitivity Tests; D058885:Multilocus Sequence Typing; D013203:Staphylococcal Infections / Q000382:microbiology*; D041162:Staphylococcus haemolyticus / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D020080:Tandem Repeat Sequences
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial
keywords: Blood culture; MLVF typing; Multidrug resistance; Pediatric patients; S. haemolyticus