title: Circulating autoantibodies in cancer patients have high specificity for glycoxidation modified histone H2A.
abstract: BACKGROUND
  Novel immunological epitopes upon glyoxidation modified proteins have been discovered and multi-specific natural antibodies against them have been identified. The association of glyoxidation with cancer has also been reported. We probed the link of glyoxidation of histone H2A with autoimmune response in cancer.
  
  
  METHODS
  We report the formation of thermo stable amorphous aggregate formation in histone H2A upon methylglyoxal modification using circular dichroism (CD) analysis and transmission electron microscopy.
  
  
  RESULTS
  The modified histone was found to be highly immunogenic that generates specific immune response in rabbits as analysed by cross reaction studies by competitive ELISA and Western blotting technique. The anti-methylglyoxal modified H2A antibodies were found in the circulating autoantibodies in various types of cancer patients by immunosorbent assay and gel retardation studies. The results clearly indicate the formation of highly specific antibodies in cancer patients against glyoxidation modified histone H2A with cross reactive tendencies with other glycated proteins and nucleic acids.
  
  
  CONCLUSIONS
  The results are important because a link between AGE-RAGE axis (Advanced glycation end products and Receptors for AGEs) and carcinogenesis is emerging and the role of glyoxidation of proteins is expected in the development of biomarkers for the early detection of cancer.
authors.affiliation: Department of Biochemistry, Jawaharlal Nehru Medical College, Faculty of Medicine, Aligarh Muslim University, Aligarh, Uttar Pradesh, India. Electronic address: roufonline@gmail.com.
authors.forename: Abdul Rouf
authors.initials: AR
authors.lastname: Mir
authors.affiliation: Department of Biochemistry, Jawaharlal Nehru Medical College, Faculty of Medicine, Aligarh Muslim University, Aligarh, Uttar Pradesh, India.
authors.lastname: Moinuddin
authors.affiliation: Department of Biochemistry, Jawaharlal Nehru Medical College, Faculty of Medicine, Aligarh Muslim University, Aligarh, Uttar Pradesh, India.
authors.forename: Sidra
authors.initials: S
authors.lastname: Islam
mesh_terms: D000328:Adult; D000818:Animals; D000918:Antibody Specificity; D001323:Autoantibodies / Q000097:blood* / Q000276:immunology*; D002417:Cattle; D005260:Female; D017127:Glycation End Products, Advanced / Q000378:metabolism; D006657:Histones / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism*; D006801:Humans; D008297:Male; D009369:Neoplasms / Q000097:blood*; D011489:Protein Denaturation; D011765:Pyruvaldehyde / Q000378:metabolism; D011817:Rabbits; D013696:Temperature
chemical_list: D001323:Autoantibodies; D017127:Glycation End Products, Advanced; D006657:Histones; D011765:Pyruvaldehyde
keywords: Autoimmunity; Cancer; Glyoxidation; Histone H2A