title: [Genetic stability of the HA, NA, and NS genes of the recombinant vector virus Flu-NS1-124-Omp16 (H5N1) expressing the brucellar gene].
abstract: The recombinant strain Flu-NS1-124-Omp16 (H5N1) of the influenza virus expressing the brucellar Omp16 gene was constructed on the basis of the technology of reverse genetics for the purpose of developing vector anti-brucellosis vaccine. The obtained recombinant strain is a genetically stable construction. This stability is confirmed by the comparative analysis of the nucleotide sequences of the HA, NA, and NS genes of the recombinant vector virus Flu-NS1-124-Omp16 (H5N1) expressing the Omp16 gene of the Brucella abortus (GenBank: AAA59360.1). The comparative analysis showed that the nucleotide sequence of the NS gene of the first and the fifth passage level of the Flu-NS1-124-Omp16 (H5N1) virus corresponded for 100% to the initial part of 12AAS2TC_124 Omp16g containing the chimera NS1-124-Omp16 in the composition of DNA (deoxyribonucleic acid) plasmids pHW2000. Total identity with HA and NA genes of the strain A/AstanaRG/6:2/2009 (H5N1) was shown by the comparative analysis of the nucleotide sequences of HA and NA genes of the first and the fifth passage level of the recombinant strain Flu-NS1-124-Omp16 (H5N1). The recombinant vector virus Flu-NS1-124-Omp16 (H5N1) expressing the brucella Omp16 gene maintains the genetic stability during 5 passages in 10-day developing chicken embryos.
authors.forename: S O
authors.initials: SO
authors.lastname: Sadikaliyeva
authors.forename: K T
authors.initials: KT
authors.lastname: Sultankulova
authors.forename: K A
authors.initials: KA
authors.lastname: Shorayeva
authors.forename: V M
authors.initials: VM
authors.lastname: Strochkov
authors.forename: O V
authors.initials: OV
authors.lastname: Chervyakova
authors.forename: V L
authors.initials: VL
authors.lastname: Zaitsev
authors.forename: K K
authors.initials: KK
authors.lastname: Tabynov
authors.forename: N T
authors.initials: NT
authors.lastname: Sandybayev
authors.forename: A R
authors.initials: AR
authors.lastname: Sansyzbay
authors.forename: A Yu
authors.initials: AY
authors.lastname: Egorov
mesh_terms: D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000378:metabolism; D002003:Brucella abortus / Q000235:genetics*; D002642:Chick Embryo; D005798:Genes, Bacterial; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics / Q000378:metabolism; D053124:Influenza A Virus, H5N1 Subtype / Q000235:genetics / Q000378:metabolism; D009439:Neuraminidase / Q000235:genetics / Q000378:metabolism; D017361:Viral Nonstructural Proteins; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D001425:Bacterial Outer Membrane Proteins; D019267:Hemagglutinin Glycoproteins, Influenza Virus; C108309:NS protein, influenza virus; D017361:Viral Nonstructural Proteins; D014764:Viral Proteins; C487630:NA protein, influenza A virus; D009439:Neuraminidase