title: Molecular Aspects of the Interaction of Iminium and Alkanolamine Forms of the Anticancer Alkaloid Chelerythrine with Plasma Protein Bovine Serum Albumin.
abstract: The interaction between a quaternary benzophenanthridine alkaloid chelerythrine (herein after, CHL) and bovine serum albumin (herein after, BSA) was probed by employing various spectroscopic tools and isothermal titration calorimetry (ITC). Fluorescence studies revealed that the binding affinity of the alkanolamine form of the CHL is higher compared to the iminium counterpart. This was further established by fluorescence polarization anisotropy measurement and ITC. Fluorescence quenching study along with time-resolved fluorescence measurements establish that both forms of CHL quenched the fluorescence intensity of BSA through the mechanism of static quenching. Site selective binding and molecular modeling studies revealed that the alkaloid binds predominantly in the BSA subdomain IIA by electrostatic and hydrophobic forces. From Forster resonance energy transfer (FRET) studies, the average distances between the protein donor and the alkaloid acceptor were found to be 2.71 and 2.30 nm between tryptophan (Trp) 212 (donor) and iminium and alkanolamine forms (acceptor), respectively. Circular dichroism (CD) study demonstrated that the α-helical organization of the protein is reduced due to binding with CHL along with an increase in the coiled structure. This is indicative of a small but definitive partial unfolding of the protein. Thermodynamic parameters obtained from ITC experiments revealed that the interaction is favored by negative enthalpy change and positive entropy change.
authors.affiliation: Department of Chemistry, Jadavpur University , Raja S. C. Mullick Road, Jadavpur, Kolkata 700 032, India.
authors.forename: Sutanwi
authors.initials: S
authors.lastname: Bhuiya
authors.affiliation: Department of Chemistry, Jadavpur University , Raja S. C. Mullick Road, Jadavpur, Kolkata 700 032, India.
authors.forename: Ankur Bikash
authors.initials: AB
authors.lastname: Pradhan
authors.affiliation: Department of Chemistry, Jadavpur University , Raja S. C. Mullick Road, Jadavpur, Kolkata 700 032, India.
authors.forename: Lucy
authors.initials: L
authors.lastname: Haque
authors.affiliation: Department of Chemistry, Jadavpur University , Raja S. C. Mullick Road, Jadavpur, Kolkata 700 032, India.
authors.forename: Suman
authors.initials: S
authors.lastname: Das
mesh_terms: D000470:Alkaloids / Q000737:chemistry*; D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry*; D053119:Benzophenanthridines / Q000737:chemistry*; D002151:Calorimetry; D002417:Cattle; D007097:Imines / Q000737:chemistry*; D062105:Molecular Docking Simulation; D015394:Molecular Structure; D012710:Serum Albumin, Bovine / Q000737:chemistry*
chemical_list: D000470:Alkaloids; D000970:Antineoplastic Agents; D053119:Benzophenanthridines; D007097:Imines; D012710:Serum Albumin, Bovine; C016299:chelerythrine