title: Infection of human keratinocytes by Streptococcus dysgalactiae subspecies dysgalactiae isolated from milk of the bovine udder.
abstract: Streptococcus dysgalactiae subsp. dysgalactiae (SDSD) are considered exclusive animal pathogens; however, a putative zoonotic upper limb cellulitis, a prosthetic joint infection and an infective endocarditis were described in humans. To unravel if bovine SDSD isolates are able to infect human cells, the adherence and internalization to human primary keratinocytes of two bovine SDSD strains isolated from milk collected from udder were analyzed. Bacterial adhesion assays and confocal microscopy indicate a high adherence and internalization of SDSD isolates to human cells, suggesting for the first time the ability of bovine isolates to infect human cells.
authors.affiliation: UCIBIO, Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Campus de Caparica, 2829-516 Caparica, Portugal.
authors.forename: Catarina
authors.initials: C
authors.lastname: Roma-Rodrigues
authors.affiliation: UCIBIO, Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Campus de Caparica, 2829-516 Caparica, Portugal.
authors.forename: Cynthia
authors.initials: C
authors.lastname: Alves-Barroco
authors.affiliation: UCIBIO, Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Campus de Caparica, 2829-516 Caparica, Portugal; CQE, Centro de Quimica Estrutural, Complexo 1, Instituto Superior Técnico, Universidade de Lisboa, Avenida Rovisco Pais, 1049-001 Lisboa, Portugal.
authors.forename: Luís R
authors.initials: LR
authors.lastname: Raposo
authors.affiliation: i3N/CENIMAT, Departamento de Ciência dos Materiais, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Campus de Caparica, 2829-516 Caparica, Portugal.
authors.forename: Mafalda N
authors.initials: MN
authors.lastname: Costa
authors.affiliation: i3N/CENIMAT, Departamento de Ciência dos Materiais, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Campus de Caparica, 2829-516 Caparica, Portugal.
authors.forename: Elvira
authors.initials: E
authors.lastname: Fortunato
authors.affiliation: UCIBIO, Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Campus de Caparica, 2829-516 Caparica, Portugal.
authors.forename: Pedro Viana
authors.initials: PV
authors.lastname: Baptista
authors.affiliation: UCIBIO, Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Campus de Caparica, 2829-516 Caparica, Portugal; CQE, Centro de Quimica Estrutural, Complexo 1, Instituto Superior Técnico, Universidade de Lisboa, Avenida Rovisco Pais, 1049-001 Lisboa, Portugal. Electronic address: ma.fernandes@fct.unl.pt.
authors.forename: Alexandra R
authors.initials: AR
authors.lastname: Fernandes
authors.affiliation: UCIBIO, Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Campus de Caparica, 2829-516 Caparica, Portugal. Electronic address: isanches@fct.unl.pt.
authors.forename: Ilda
authors.initials: I
authors.lastname: Santos-Sanches
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion; D002417:Cattle; D002478:Cells, Cultured; D004705:Endocytosis; D006801:Humans; D015603:Keratinocytes / Q000382:microbiology*; D008321:Mammary Glands, Animal / Q000382:microbiology; D018613:Microscopy, Confocal; D008892:Milk / Q000382:microbiology*; D013291:Streptococcus / Q000302:isolation & purification / Q000472:pathogenicity*
keywords: Adhesion; Bovine; Host; Human keratinocytes; Internalization; Streptococcus dysgalactiae subspecies dysgalactiae