title: Determination of diethylstilbestrol in seawater by molecularly imprinted solid-phase extraction coupled with high-performance liquid chromatography.
abstract: An effective and highly selective molecularly imprinted material was prepared by suspension polymerization for the isolation and pre-concentration of synthetic estrogen diethylstilbestrol (DES) in seawater. The obtained MIPMs were proved to have more uniform size and porous structure, with maximum adsorption capacity of 8.43 mg g(-1) almost two times more than NIPMs (4.43 mg g(-1)). The MIPMs showed no significant deterioration of the adsorption capacity after five rounds of regeneration. An off-line molecularly imprinted solid phase extraction (MISPE) method followed by HPLC-DAD was proposed for the detection of DES in seawater, and recoveries were satisfactorily higher than 77%. Four seawater samples in aquaculture area were analyzed and 0.61 ng mL(-1) DES was detected in one sample. The result demonstrated that this method can be used for the rapid separation and clean up of trace residual of DES in seawater.
authors.affiliation: Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao 266100, PR China.
authors.forename: Xiuping
authors.initials: X
authors.lastname: He
authors.affiliation: Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao 266100, PR China.
authors.forename: Xiaoqi
authors.initials: X
authors.lastname: Mei
authors.affiliation: Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao 266100, PR China. Electronic address: jtwang@ouc.edu.cn.
authors.forename: Jiangtao
authors.initials: J
authors.lastname: Wang
authors.affiliation: Marine College, Shandong University, Weihai 264209, PR China.
authors.forename: Ziru
authors.initials: Z
authors.lastname: Lian
authors.affiliation: Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao 266100, PR China.
authors.forename: Liju
authors.initials: L
authors.lastname: Tan
authors.affiliation: Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao 266100, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wu
mesh_terms: D000327:Adsorption; D017756:Aquaculture; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004054:Diethylstilbestrol / Q000032:analysis* / Q000737:chemistry; D004784:Environmental Monitoring / Q000379:methods*; D054802:Molecular Imprinting / Q000379:methods; D058105:Polymerization; D012623:Seawater / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000379:methods
chemical_list: D004054:Diethylstilbestrol
keywords: Diethylstilbestrol; High-performance liquid chromatography; Molecularly imprinted solid-phase extraction; Seawater