title: Monitoring the effects of treatment in colon cancer cells using immunohistochemical and histoenzymatic techniques.
abstract: BACKGROUND
  Monitoring the effects of treatment in malignant diseases is very important in study of the influence on the cell metabolism. Energy production in cancer cells is abnormally dependent on aerobic glycolysis. In addition to the dependency on glycolysis, cancer cells have other atypical metabolic characteristics. The purpose of the present study is to evaluation and analysis of the colon cancer cells under anti-angiogenic treatment, to establish the changes in the cellular energy metabolism and apoptotic potential. Anti-angiogenic drugs block the vascular endothelial growth factors, preventing the formation of new vessels.
  
  
  METHODS
  We use immunohistochemical analysis of cytochrome c release and histoenzymatic analysis of adenosine triphosphatase (ATP-ase), succinate dehydrogenase (SDH), lactate dehydrogenase (LDH) enzymes. Colorectal tumor tissue samples were obtained by biopsy following the surgical procedures at the County Clinical Hospital of Oradea (Romania).
  
  
  RESULTS
  The obtained results show that the apoptotic potential of malignant cells increases during the anti-angiogenic treatment, in the same time the rate of glycolysis increases, due to installed hypoxia and reduced ATP synthesis. Our results have been confirmed by international studies too.
  
  
  CONCLUSIONS
  It was been demonstrated that the apoptotic potential of malignant cells increases significantly during anti-angiogenic treatment. There is growing evidence that cancer's "Achilles' heel" is tumor cell metabolism.
authors.affiliation: Department of Pharmacy, Faculty of Medicine and Pharmacy, University of Oradea, Romania; simonabungau@gmail.com.
authors.forename: Annamaria
authors.initials: A
authors.lastname: Pallag
authors.forename: Elena
authors.initials: E
authors.lastname: Roşca
authors.forename: Delia Mirela
authors.initials: DM
authors.lastname: ţiŢ
authors.forename: Gabriela
authors.initials: G
authors.lastname: MuŢiu
authors.forename: Simona Gabriela
authors.initials: SG
authors.lastname: Bungău
authors.forename: Ovidiu Laurean
authors.initials: OL
authors.lastname: Pop
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism; D017209:Apoptosis; D003110:Colonic Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology*; D045304:Cytochromes c / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D005260:Female; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009362:Neoplasm Metastasis; D013385:Succinate Dehydrogenase / Q000378:metabolism
chemical_list: D045304:Cytochromes c; D007770:L-Lactate Dehydrogenase; D013385:Succinate Dehydrogenase; D000251:Adenosine Triphosphatases