title: Intradermal injection of human adipose-derived stem cells accelerates skin wound healing in nude mice.
abstract: BACKGROUND
  The use of stem cells from adipose tissue or adipose-derived stem cells (ASCs) in regenerative medicine could be an interesting alternative to bone marrow stem cells because they are easily accessible and available in large quantities. The aim of this study was to evaluate the potential effect of ASCs on the healing of 12 mm diameter-excisional wounds (around 110 mm(2)) in nude mice.
  
  
  METHODS
  Thirty nude mice underwent surgery to create one 12-mm excisional wound per mouse (spontaneous healing, n = 6; Cytocare® 532, n = 12; ASCs, n = 12). The Galiano wound model was chosen to avoid shrinkage and thus slow the spontaneous healing (SH) of mouse skin, making it closer to the physiology of human skin healing. Transparent dressings were used to enable daily healing time measurements to be taken. Immunohistochemistry, histological and blood perfusion analysis were carried out on the healed skin.
  
  
  RESULTS
  The in vivo results showed the effectiveness of using ASCs on reducing the time needed for complete healing to 21.2 days for SH, 17.4 days for vehicle alone (Cytocare® 532) and 14.6 days with the addition of ASCs (p < 0.001). Moreover, cutaneous perfusion of the healed wound was significantly improved in ASC-treated mice compared to SH group, as shown by laser Doppler flowmetry and the quantitation of blood vessels using immunohistochemistry of αsmooth muscle actin.
  
  
  CONCLUSIONS
  The tolerance and efficacy of cryopreserved ASCs to accelerate the complete closure of the wound by increasing the maturation of the skin and its blood perfusion, shows their therapeutic benefit in the wound healing context.
authors.affiliation: Banque de tissus et cellules, Laboratoire des substituts cutanés, Hôpital Edouard Herriot, Hospices Civils de Lyon, 5, place d'Arsonval, Pavillon i, 69437, Lyon, France. jonathan.rodriguez@chu-lyon.fr.|INSERM U1060, CarMeN laboratory, Oullins, France. jonathan.rodriguez@chu-lyon.fr.|Cell and Tissue Bank, Cutaneous Substitute Laboratory, Edouard Herriot Hospital, 5, place d'Arsonval, Pavillon I, 69437, Lyon, France. jonathan.rodriguez@chu-lyon.fr.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Rodriguez
authors.affiliation: Service de chirurgie plastique, esthétique et reconstructrice, Hospices Civils de Lyon, University of Lyon, Lyon, France. fabien.boucher@chu-lyon.fr.
authors.forename: Fabien
authors.initials: F
authors.lastname: Boucher
authors.affiliation: Banque de tissus et cellules, Laboratoire des substituts cutanés, Hôpital Edouard Herriot, Hospices Civils de Lyon, 5, place d'Arsonval, Pavillon i, 69437, Lyon, France. lequeuxc@cellforcure.com.|IBCP-UMR 5305 CNRS, 7 passage du Vercors, 69 367, Lyon, Cedex 07, France. lequeuxc@cellforcure.com.
authors.forename: Charlotte
authors.initials: C
authors.lastname: Lequeux
authors.affiliation: IBCP-UMR 5305 CNRS, 7 passage du Vercors, 69 367, Lyon, Cedex 07, France. audrey.josset-lamaugarny@ibcp.fr.
authors.forename: Audrey
authors.initials: A
authors.lastname: Josset-Lamaugarny
authors.affiliation: Banque de tissus et cellules, Laboratoire des substituts cutanés, Hôpital Edouard Herriot, Hospices Civils de Lyon, 5, place d'Arsonval, Pavillon i, 69437, Lyon, France. ondine.rouyer@gmail.com.|IBCP-UMR 5305 CNRS, 7 passage du Vercors, 69 367, Lyon, Cedex 07, France. ondine.rouyer@gmail.com.
authors.forename: Ondine
authors.initials: O
authors.lastname: Rouyer
authors.affiliation: Banque de tissus et cellules, Laboratoire des substituts cutanés, Hôpital Edouard Herriot, Hospices Civils de Lyon, 5, place d'Arsonval, Pavillon i, 69437, Lyon, France. ardisson.orianne@gmail.com.|IBCP-UMR 5305 CNRS, 7 passage du Vercors, 69 367, Lyon, Cedex 07, France. ardisson.orianne@gmail.com.
authors.forename: Orianne
authors.initials: O
authors.lastname: Ardisson
authors.affiliation: Laboratoire Central d'Anatomie Pathologique, Hôpital Édouard Herriot, Lyon, France. helena.rutschi@chu-lyon.fr.
authors.forename: Héléna
authors.initials: H
authors.lastname: Rutschi
authors.affiliation: IBCP-UMR 5305 CNRS, 7 passage du Vercors, 69 367, Lyon, Cedex 07, France. dominique.sigaudo-roussel@ibcp.fr.
authors.forename: Dominique
authors.initials: D
authors.lastname: Sigaudo-Roussel
authors.affiliation: Banque de tissus et cellules, Laboratoire des substituts cutanés, Hôpital Edouard Herriot, Hospices Civils de Lyon, 5, place d'Arsonval, Pavillon i, 69437, Lyon, France. odile.damour@chu-lyon.fr.|IBCP-UMR 5305 CNRS, 7 passage du Vercors, 69 367, Lyon, Cedex 07, France. odile.damour@chu-lyon.fr.
authors.forename: Odile
authors.initials: O
authors.lastname: Damour
authors.affiliation: INSERM U1060, CarMeN laboratory, Oullins, France. dr.mojallal@gmail.com.|Service de chirurgie plastique, esthétique et reconstructrice, Hospices Civils de Lyon, University of Lyon, Lyon, France. dr.mojallal@gmail.com.
authors.forename: Ali
authors.initials: A
authors.lastname: Mojallal
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D002921:Cicatrix; D006801:Humans; D007271:Injections, Intradermal; D008297:Male; D051379:Mice; D008819:Mice, Nude; D012867:Skin / Q000098:blood supply / Q000293:injuries*; D033581:Stem Cell Transplantation; D013997:Time Factors; D014945:Wound Healing