title: The effects of proinflammatory cytokines on the apoptosis of corneal endothelial cells following argon laser iridotomy.
abstract: The aim of this study was to evaluate the relationship between the expression of proinflammatory cytokines and the apoptosis of corneal endothelial cells after argon laser iridotomy (ALI). ALI was performed on each quadrant of the iris in the right eye of mice (ALI1 group). Left eyes were used as control group. The levels of interleukin (IL)-1β, tumor necrosis factor (TNF)-α, transforming growth factor (TGF)-β, and interferon (IFN)-γ in mice eyes were measured, and TUNEL staining was performed 12 h after ALI. Mice in the ALI-Dexa group were pretreated daily with an intraperitoneal injection of dexamethasone for 4 days before undergoing ALI and compared with mice without dexamethasone pretreatment (ALI2 group). Twelve corneas from six rabbits were incubated ex vivo with (n = 6) or without (n = 6) IL-1β. TUNEL staining was performed 24 h after ex vivo incubation. In the mice experiment, the levels of IL-1β, TNF-α, TGF-β, and IFN-γ were increased in the ALI1 group compared to the control group. Although many TUNEL-positive cells were observed in the ALI1 group, those were not detected in the control group. Dexamethasone pretreatment inhibited the increase in the levels of all four proinflammatory cytokines and reduced TUNEL-positive cells. In the rabbit experiment, TUNEL-positive cells were increased in the incubated corneas with IL-1β compared to those without IL-1β. Expression of proinflammatory cytokines following ALI seems to play a role in the apoptosis of corneal endothelial cells after ALI. Dexamethasone pretreatment inhibited increases in proinflammatory cytokines and reduced the apoptosis of corneal endothelial cells.
authors.affiliation: Department of Ophthalmology, Ansan Hospital, Korea University College of Medicine, 516, Gojan 1-dong, Danwon-gu, Ansan-si, 425-707, Gyeonggi, South Korea.
authors.forename: Youngsub
authors.initials: Y
authors.lastname: Eom
authors.affiliation: Department of Ophthalmology, Guro Hospital, Korea University College of Medicine, 80, Guro-dong, Guro-gu, 152-703, Seoul, South Korea.
authors.forename: Junki
authors.initials: J
authors.lastname: Kwon
authors.affiliation: Department of Ophthalmology, Guro Hospital, Korea University College of Medicine, 80, Guro-dong, Guro-gu, 152-703, Seoul, South Korea.
authors.forename: Jeong-Hwa
authors.initials: JH
authors.lastname: Heo
authors.affiliation: Department of Ophthalmology, Guro Hospital, Korea University College of Medicine, 80, Guro-dong, Guro-gu, 152-703, Seoul, South Korea.
authors.forename: Cheolmin
authors.initials: C
authors.lastname: Yun
authors.affiliation: Department of Ophthalmology, Anam Hospital, Korea University College of Medicine, 126-1, Anam-dong 5-ga, Seongbuk-gu, 136-705, Seoul, South Korea.
authors.forename: Su-Yeon
authors.initials: SY
authors.lastname: Kang
authors.affiliation: Department of Ophthalmology, Anam Hospital, Korea University College of Medicine, 126-1, Anam-dong 5-ga, Seongbuk-gu, 136-705, Seoul, South Korea.
authors.forename: Hyo Myung
authors.initials: HM
authors.lastname: Kim
authors.affiliation: Department of Ophthalmology, Guro Hospital, Korea University College of Medicine, 80, Guro-dong, Guro-gu, 152-703, Seoul, South Korea. Electronic address: crisim@korea.ac.kr.
authors.forename: Jong Suk
authors.initials: JS
authors.lastname: Song
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D015153:Blotting, Western; D002478:Cells, Cultured; D055954:Corneal Endothelial Cell Loss / Q000209:etiology / Q000378:metabolism* / Q000473:pathology; D016207:Cytokines / Q000378:metabolism*; D004195:Disease Models, Animal; D004728:Endothelium, Corneal / Q000378:metabolism* / Q000473:pathology; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D007498:Iris / Q000601:surgery*; D053685:Laser Therapy / Q000009:adverse effects*; D054020:Lasers, Gas / Q000627:therapeutic use; D051379:Mice; D008810:Mice, Inbred C57BL; D013508:Ophthalmologic Surgical Procedures / Q000009:adverse effects*; D011817:Rabbits
chemical_list: D016207:Cytokines
keywords: Apoptosis; Argon laser iridotomy; Corneal decompensation; Corneal endothelial cell; Proinflammatory cytokine