title: Electrically Activated Primary Human Fibroblasts Improve In Vitro and In Vivo Skin Regeneration.
abstract: Electrical stimulation (ES) changes cellular behaviors and thus constitutes a potential strategy to promote wound healing. However, well-controlled in vitro findings have yet to be translated to in vivo trials. This study was to demonstrate the feasibility and advantages of transplanting electrically activated cells (E-Cells) to help wound healing. Primary human skin fibroblasts were activated through well defined ES and cultured with keratinocytes to generate engineered human skin (EHS), which were transplanted to nu/nu mice. The electrically activated EHS grafts were analyzed at 20 and 30 days post-grafting, showing faster wound closure, thick epidermis, vasculature, and functional basement membrane containing laminin and type IV collagen that were totally produced by the implanted human cells. Because a variety of cells can be electrically activated, E-Cells may become a new cell source and the transplantation of E-Cells may represent a new strategy in wound healing and tissue engineering. J. Cell. Physiol. 231: 1814-1821, 2016. © 2015 Wiley Periodicals, Inc.
authors.affiliation: Groupe de Recherche en Écologie Buccale, Faculté de Médecine Dentaire, Université Laval, Québec, Canada.
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Rouabhia
authors.affiliation: Groupe de Recherche en Écologie Buccale, Faculté de Médecine Dentaire, Université Laval, Québec, Canada.|Département de Chirurgie, Faculté de Médecine, Axe Médecine Régénératrice, Centre de Recherche du CHU de Québec, Université Laval, Québec, Canada.
authors.forename: Hyun Jin
authors.initials: HJ
authors.lastname: Park
authors.affiliation: Département de Chirurgie, Faculté de Médecine, Axe Médecine Régénératrice, Centre de Recherche du CHU de Québec, Université Laval, Québec, Canada.
authors.forename: Ze
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D001485:Basement Membrane / Q000378:metabolism; D002450:Cell Communication; D002478:Cells, Cultured; D018920:Coculture Techniques; D024141:Collagen Type IV / Q000378:metabolism; D004558:Electric Stimulation; D005240:Feasibility Studies; D005347:Fibroblasts / Q000378:metabolism / Q000473:pathology / Q000637:transplantation*; D064593:Heterografts; D006801:Humans; D015603:Keratinocytes / Q000378:metabolism / Q000473:pathology / Q000637:transplantation*; D007797:Laminin / Q000378:metabolism; D008297:Male; D008819:Mice, Nude; D010641:Phenotype; D012867:Skin / Q000378:metabolism / Q000473:pathology*; D016038:Skin Transplantation; D013997:Time Factors; D023822:Tissue Engineering / Q000379:methods*; D014945:Wound Healing; D055815:Young Adult
chemical_list: D024141:Collagen Type IV; D007797:Laminin