title: Selective production of 1-monocaprin by porcine liver carboxylesterase-catalyzed esterification: Its enzyme kinetics and catalytic performance.
abstract: Porcine liver carboxylesterase (PLE) belongs to carboxylesterase family (EC 3.1.1.1) as a serine-type esterase. The PLE-catalyzed esterification of capric acid with glycerol in reverse micelles was investigated on the catalytic performance and enzyme kinetics. The most suitable structure of reverse micelles was comprised of isooctane (reaction medium) and bis(2-ethylhexyl) sodium sulfosuccinate (AOT, anionic surfactant) with 0.1 of R-value ([water]/[surfactant]) and 3.0 of G/F-value ([glycerol]/[fatty acid]) for the PLE-catalyzed esterification. In the aspect of regio-selectivity, the PLE mainly produced 1-monocaprin without any other products (di- and/or tricaprins of subsequent reactions). Furthermore, the degree of esterification at equilibrium state (after 4 h from the initiation) was 62.7% under the optimum conditions at pH 7.0 and 60 °C. Based on Hanes-Woolf plot, the apparent Km and Vmax values were calculated to be 16.44 mM and 38.91 μM/min/mg protein, respectively.
authors.affiliation: Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Republic of Korea.
authors.forename: Kyung-Min
authors.initials: KM
authors.lastname: Park
authors.affiliation: Research Institute of Food and Biotechnology, SPC group, Seoul 137-887, Republic of Korea.
authors.forename: Jong-Hyuk
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Republic of Korea.
authors.forename: Sung-Chul
authors.initials: SC
authors.lastname: Hong
authors.affiliation: Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Republic of Korea.
authors.forename: Chang Woo
authors.initials: CW
authors.lastname: Kwon
authors.affiliation: Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Republic of Korea.
authors.forename: Minje
authors.initials: M
authors.lastname: Jo
authors.affiliation: Department of Food Science and Technology, and Department of Interdisciplinary Bio IT Materials, Seoul National University of Science and Technology, Seoul 139-743, Republic of Korea.
authors.forename: Seung Jun
authors.initials: SJ
authors.lastname: Choi
authors.affiliation: Institute of Advanced Machinery and Design, School of Mechanical and Aerospace Engineering, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Keesung
authors.initials: K
authors.lastname: Kim
authors.affiliation: Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Republic of Korea; Center for Food and Bioconvergence, and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 151-742, Republic of Korea. Electronic address: pschang@snu.ac.kr.
authors.forename: Pahn-Shick
authors.initials: PS
authors.lastname: Chang
mesh_terms: D000818:Animals; D043182:Carboxylesterase / Q000378:metabolism*; D002384:Catalysis; D003652:Decanoic Acids / Q000378:metabolism; D004951:Esterification; D005989:Glycerides / Q000096:biosynthesis* / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008823:Micelles; D012997:Solvents; D013501:Surface-Active Agents; D013552:Swine; D013696:Temperature
chemical_list: D003652:Decanoic Acids; D005989:Glycerides; D008823:Micelles; D012997:Solvents; D013501:Surface-Active Agents; C004204:rac-glycerol 1-monodecanoate; C031071:decanoic acid; D043182:Carboxylesterase
keywords: 1-monocaprin; Enzyme kinetics; Esterification; Porcine liver carboxylesterase; Reverse micelles