title: Multifunctional Microspheres Encoded with Upconverting Nanocrystals and Magnetic Nanoparticles for Rapid Separation and Immunoassays.
abstract: Immunoassays based on the downconversion target materials (organic dyes or quantum dots) lead to fairly strong spectral interference between the coded signal and reporter signal, which seriously affects the detection accuracy and hampers their applications. In this work, a new kind of upconverting nanocrystals encoded magnetic microspheres (UCNMMs) were designed and prepared successfully to solve the problem mentioned above. The UCNMMs were obtained by incorporating magnetic Fe3O4 nanoparticles and upconverting nanocrystals with polystyrene microspheres. Due to that upconverting nanocrystals (UCNs) and reporter signals are excitated by near-infrared and UV/visible light separately, immunoassays based on UCNMMs do not occur optical spectral interferences. Furthermore, these new functionalized UCNMMs have excellent properties in binding biomolecules and fast separating, which would have large potential applications in multiplexed assays.
authors.affiliation: Institute of Nanobiotechnology, School of Life Sciences, Tianjin University , Tianjin, 300072, People's Republic of China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Institute of Nanobiotechnology, School of Materials Science and Engineering, Tianjin University , Tianjin, 300072, People's Republic of China.
authors.forename: Chunhong
authors.initials: C
authors.lastname: Dong
authors.affiliation: Institute of Nanobiotechnology, School of Materials Science and Engineering, Tianjin University , Tianjin, 300072, People's Republic of China.
authors.forename: Lin
authors.initials: L
authors.lastname: Su
authors.affiliation: Institute of Nanobiotechnology, School of Life Sciences, Tianjin University , Tianjin, 300072, People's Republic of China.
authors.forename: Hanjie
authors.initials: H
authors.lastname: Wang
authors.affiliation: Institute of Nanobiotechnology, School of Life Sciences, Tianjin University , Tianjin, 300072, People's Republic of China.
authors.forename: Xiaoqun
authors.initials: X
authors.lastname: Gong
authors.affiliation: School of Electronics and Information Engineering, Tianjin Polytechnic University , Tianjin 300387, People's Republic of China.
authors.forename: Huiquan
authors.initials: H
authors.lastname: Wang
authors.affiliation: Institute of Nanobiotechnology, School of Materials Science and Engineering, Tianjin University , Tianjin, 300072, People's Republic of China.
authors.forename: Junqing
authors.initials: J
authors.lastname: Liu
authors.affiliation: Institute of Nanobiotechnology, School of Life Sciences, Tianjin University , Tianjin, 300072, People's Republic of China.
authors.forename: Jin
authors.initials: J
authors.lastname: Chang
mesh_terms: D007118:Immunoassay / Q000379:methods*; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D008863:Microspheres; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D045663:Quantum Dots; D013050:Spectrometry, Fluorescence
chemical_list: D058185:Magnetite Nanoparticles
keywords: double-antibody sandwich immunoassay; fast immunoassay; magnetic nanoparticles; multifunctional microspheres; upconverting nanocrystals