title: In vitro antitumor effects of two novel oligostilbenes, cis- and trans-suffruticosol D, isolated from Paeonia suffruticosa seeds.
abstract: Naturally derived stilbenes have been shown to elicit cytotoxic, anti-steroidal, anti-mutagenic, anti-oxidative, anti-inflammatory, and antitumor bioactivities. Previous phytochemical studies revealed that the seeds of Paeonia suffruticosa are rich in natural stilbenes. In this study the antitumor effects and mechanism of action of the oligostilbene isomers, cis- and trans-suffruticosol D, isolated from the seeds of P. suffruticosa were examined. cis- and trans-suffruticosol D exhibited remarkable cytotoxicity against the human cancer cell lines A549 (lung), BT20 (breast), MCF-7 (breast), and U2OS (osteosarcoma), but showed significantly less toxicity to the normal human cell lines HMEC (breast) and HPL1A (lung). We also demonstrated that cis- and trans-suffruticosol D exerted their antitumor effects by provoking oxidative stress, stimulating apoptosis, decreasing the mitochondrial membrane potential, inhibiting cell motility, and blocking the NF-κB pathway in human lung cancer cells. In addition, we evaluated their respective bioefficacy and found that trans-suffruticosol D is more potent than cis-suffruticosol D. Collectively, our results suggest that cis- and trans-suffruticosol D could be promising chemotherapeutic agents against cancer.
authors.affiliation: Tennessee Center for Botanical Medicine Research and The Department of Biology, Middle Tennessee State University, Murfreesboro, TN 37132, USA.
authors.forename: Nadin Marwan
authors.initials: NM
authors.lastname: Almosnid
authors.affiliation: Tennessee Center for Botanical Medicine Research and The Department of Biology, Middle Tennessee State University, Murfreesboro, TN 37132, USA.
authors.forename: Ying
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Haidian, Beijing 100193, P.R. China.
authors.forename: Chunnian
authors.initials: C
authors.lastname: He
authors.affiliation: Tennessee Center for Botanical Medicine Research and The Department of Biology, Middle Tennessee State University, Murfreesboro, TN 37132, USA.
authors.forename: Hyo Sim
authors.initials: HS
authors.lastname: Park
authors.affiliation: Tennessee Center for Botanical Medicine Research and The Department of Biology, Middle Tennessee State University, Murfreesboro, TN 37132, USA.
authors.forename: Elliot
authors.initials: E
authors.lastname: Altman
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D006027:Glycosides / Q000494:pharmacology; D006801:Humans; D061986:MCF-7 Cells; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D039821:Monoterpenes / Q000494:pharmacology; D016328:NF-kappa B / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D029596:Paeonia / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry*; D013267:Stilbenes / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D006027:Glycosides; D039821:Monoterpenes; D016328:NF-kappa B; D013267:Stilbenes