title: Determination of nicotine, tar, volatile organic compounds and carbonyls in mainstream cigarette smoke using a glass filter and a sorbent cartridge followed by the two-phase/one-pot elution method with carbon disulfide and methanol.
abstract: We have developed a new analytical method for the determination of nicotine, tar, volatile organic compounds and carbonyls in main-stream cigarette smoke using a sorbent cartridge packed with Carboxen 572 (CX-572) and a Cambridge filter pad (CFP) followed by the two-phase/one-pot elution method. A CX-572 cartridge is installed between the intake of the CFP and the pump of the smoking machine. Gaseous compounds collected with the CX-572 cartridge and total particulate matter (TPM) collected with the CFP are coeluted simultaneously in the same vial and then analyzed by high-performance liquid chromatography (HPLC), gas chromatography-mass spectrometry (GC/MS) and gas chromatograph-thermal conductivity detector (GC/TCD). Carbonyl compounds are determined by adding derivatizing reagent (2,4-dinitrophenylhydrazine, DNPH) to the eluate followed by HPLC analysis. VOCs and nicotine are determined by GC/MS, and water is determined by GC/TCD. The same sample eluate solution is used for HPLC, GC/MS and GC/TCD analyses. As a result of measuring main-stream cigarette smoke generated from reference cigarettes, almost all carbonyl compounds and VOCs except formaldehyde were passed through a CFP and trapped in a CX-572 cartridge. 100% of nicotine, tar and TPM were trapped in a CFP. 50% of water and 53% of formaldehyde were trapped in a CFP. The one-pot data is almost equal to the sums of CFP (particulate matter) and CX-572 (gaseous compounds) data. The two-phase/one-pot elution method can simultaneously measure nicotine, tar, volatile organic compounds and carbonyl compounds in cigarette smoke with simple operation and small amounts of reagents.
authors.affiliation: Department of Environmental Health, National Institute of Public Health, 2-3-6, Minami, Wako City, Saitama 351-0197, Japan. Electronic address: uchiyama@niph.go.jp.
authors.forename: Shigehisa
authors.initials: S
authors.lastname: Uchiyama
authors.affiliation: Chiba University Graduate School of Engineering, 1-33, Yayoicho, Inage-ku, , Chiba-shi, Chiba 263-8522, Japan.
authors.forename: Hideki
authors.initials: H
authors.lastname: Hayashida
authors.affiliation: Chiba University Graduate School of Engineering, 1-33, Yayoicho, Inage-ku, , Chiba-shi, Chiba 263-8522, Japan.
authors.forename: Rina
authors.initials: R
authors.lastname: Izu
authors.affiliation: Department of Environmental Health, National Institute of Public Health, 2-3-6, Minami, Wako City, Saitama 351-0197, Japan.
authors.forename: Yohei
authors.initials: Y
authors.lastname: Inaba
authors.affiliation: Chiba University Graduate School of Engineering, 1-33, Yayoicho, Inage-ku, , Chiba-shi, Chiba 263-8522, Japan.
authors.forename: Hideki
authors.initials: H
authors.lastname: Nakagome
authors.affiliation: Department of Environmental Health, National Institute of Public Health, 2-3-6, Minami, Wako City, Saitama 351-0197, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Kunugita
mesh_terms: D002246:Carbon Disulfide / Q000737:chemistry; D002623:Chemistry Techniques, Analytical / Q000295:instrumentation* / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D008401:Gas Chromatography-Mass Spectrometry; D000432:Methanol / Q000737:chemistry; D009538:Nicotine / Q000032:analysis; D012906:Smoke / Q000032:analysis*; D062789:Tobacco Products / Q000032:analysis; D055549:Volatile Organic Compounds / Q000032:analysis
chemical_list: D012906:Smoke; D055549:Volatile Organic Compounds; D009538:Nicotine; D002246:Carbon Disulfide; D000432:Methanol
keywords: Carbonyl compounds; Mainstream cigarette smoke; Nicotine; One-pot elution; Tar; Volatile organic compounds