title: A nontargeted study of muscle proteome in severely obese women with androgen excess compared with severely obese men and nonhyperandrogenic women.
abstract: OBJECTIVE
  Androgen excess in women is frequently associated with muscle insulin resistance, especially in obese women with polycystic ovary syndrome. However, whether this is a primary event or the result of indirect mechanisms is currently debated.
  
  
  METHODS
  This is an observational study.
  
  
  METHODS
  We obtained skeletal muscle biopsies during bariatric surgery from severely obese men (n=6) and women with (n=5) or without (n=5) androgen excess. We used two-dimensional differential gel electrophoresis and matrix-assisted laser desorption/ionization-time-of-flight/time-of-flight mass spectrometry to identify muscle proteins showing differences in abundance between the groups of obese subjects.
  
  
  RESULTS
  Women with hyperandrogenism presented the lowest abundances of glycogen phosphorylase, pyruvate kinase, β-enolase, glycerol-3-phosphate dehydrogenase, creatine kinase M-type, and desmin, whereas the abundances of these molecules were similar in control women and men.
  
  
  CONCLUSIONS
  According to our nontargeted proteomic approach, women with hyperandrogenism show a specific alteration of the skeletal muscle proteome that could contribute to their insulin resistance. Because men do not show similar results, this alteration does not appear to be the direct effect on muscle of androgen excess, but rather the consequence of indirect mechanisms that merit further studies.
authors.affiliation: DiabetesObesity and Human Reproduction Research Group, Department of Endocrinology and Nutrition, Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Centro de Investigación Biomédica en Red Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM), Hospital Universitario Ramón y Cajal, Universidad de Alcalá, Carretera de Colmenar km 9,1, E-28034 Madrid, Spain.
authors.forename: María
authors.initials: M
authors.lastname: Insenser
authors.affiliation: DiabetesObesity and Human Reproduction Research Group, Department of Endocrinology and Nutrition, Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Centro de Investigación Biomédica en Red Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM), Hospital Universitario Ramón y Cajal, Universidad de Alcalá, Carretera de Colmenar km 9,1, E-28034 Madrid, Spain.
authors.forename: Rafael
authors.initials: R
authors.lastname: Montes-Nieto
authors.affiliation: DiabetesObesity and Human Reproduction Research Group, Department of Endocrinology and Nutrition, Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Centro de Investigación Biomédica en Red Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM), Hospital Universitario Ramón y Cajal, Universidad de Alcalá, Carretera de Colmenar km 9,1, E-28034 Madrid, Spain.
authors.forename: M Ángeles
authors.initials: MÁ
authors.lastname: Martínez-García
authors.affiliation: DiabetesObesity and Human Reproduction Research Group, Department of Endocrinology and Nutrition, Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Centro de Investigación Biomédica en Red Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM), Hospital Universitario Ramón y Cajal, Universidad de Alcalá, Carretera de Colmenar km 9,1, E-28034 Madrid, Spain hectorfrancisco.escobar@salud.madrid.org.
authors.forename: Héctor F
authors.initials: HF
authors.lastname: Escobar-Morreale
mesh_terms: D000328:Adult; D050110:Bariatric Surgery; D016022:Case-Control Studies; D052283:Creatine Kinase, MM Form / Q000378:metabolism; D003893:Desmin / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D005993:Glycerolphosphate Dehydrogenase / Q000378:metabolism; D024981:Glycogen Phosphorylase / Q000378:metabolism; D006801:Humans; D017588:Hyperandrogenism / Q000378:metabolism*; D008297:Male; D013058:Mass Spectrometry; D018482:Muscle, Skeletal / Q000378:metabolism*; D009767:Obesity, Morbid / Q000378:metabolism* / Q000601:surgery; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics; D011770:Pyruvate Kinase / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D003893:Desmin; D020543:Proteome; D005993:Glycerolphosphate Dehydrogenase; D024981:Glycogen Phosphorylase; D011770:Pyruvate Kinase; D052283:Creatine Kinase, MM Form; D010751:Phosphopyruvate Hydratase