title: Measurement of hematological and serum biochemical normal values of captive housed Chlorocebus aethiops sabaeus monkeys and correlation with the age.
abstract: BACKGROUND
  Some factors such as sex, age, and captivity conditions have a direct influence on the normal hematological and serum biochemical parameters of African green monkeys. On the other hand, reliability in reported values is in many cases limited by studied animal number (<200) and there is not report on the correlation of these parameters with the age in each sex animal group. Thus, this study sought determining normal hematological (11) and serum biochemical parameters (9) of 400 captive housed African green monkeys and also correlate them with the age of the animals.
  
  
  METHODS
  A total of 200 females and 200 males were grouped by the sex and age groups (1-2, 3-4, 5-6, and 7-8 years old) for measuring normal values of hematological and serum biochemical parameters and to study the correlation of these parameters with the age of the animals.
  
  
  RESULTS
  As key outcome, the main hematological and serum biochemical reference values of African green monkeys were determined. Significant differences (P < 0.05) were found among 95% of studied parameters between males and females. About 75% and 95% of the parameters were influenced by the age in the female and male groups, respectively. About 35% of hematological and serum biochemical parameters correlated positively (R(2) > 0.5) with the age in the female monkeys. On the contrary in the male monkeys, only 45% of parameters correlated positively with the age (R(2) > 0.5).
  
  
  CONCLUSIONS
  Thus, authors believe that results of this study are important for assisting researchers in the assessment of health status of captive housed African green monkeys for preclinical studies.
authors.affiliation: Center for Genetic Engineering and Biotechnology (CIGB), Havana, Cuba.
authors.forename: Jorge
authors.initials: J
authors.lastname: Castro
authors.affiliation: Center for Genetic Engineering and Biotechnology (CIGB), Havana, Cuba.
authors.forename: Pedro
authors.initials: P
authors.lastname: Puente
authors.affiliation: Center for Genetic Engineering and Biotechnology (CIGB), Havana, Cuba.
authors.forename: Rafael
authors.initials: R
authors.lastname: Martínez
authors.affiliation: Center for Genetic Engineering and Biotechnology (CIGB), Havana, Cuba.
authors.forename: Alexander
authors.initials: A
authors.lastname: Hernández
authors.affiliation: Center for Genetic Engineering and Biotechnology (CIGB), Havana, Cuba.
authors.forename: Leticia
authors.initials: L
authors.lastname: Martínez
authors.affiliation: Center for Genetic Engineering and Biotechnology (CIGB), Havana, Cuba.
authors.forename: Dagmara
authors.initials: D
authors.lastname: Pichardo
authors.affiliation: Center for Genetic Engineering and Biotechnology (CIGB), Havana, Cuba.
authors.forename: Lizet
authors.initials: L
authors.lastname: Aldana
authors.affiliation: Center for Genetic Engineering and Biotechnology (CIGB), Havana, Cuba.
authors.forename: Iris
authors.initials: I
authors.lastname: Valdés
authors.affiliation: Center for Genetic Engineering and Biotechnology (CIGB), Havana, Cuba.
authors.forename: Karelia
authors.initials: K
authors.lastname: Cosme
mesh_terms: D000367:Age Factors; D000375:Aging / Q000097:blood*; D000818:Animals; D000830:Animals, Laboratory / Q000097:blood*; D001774:Blood Chemical Analysis / Q000662:veterinary; D001800:Blood Specimen Collection / Q000379:methods / Q000662:veterinary; D002522:Chlorocebus aethiops / Q000097:blood*; D015331:Cohort Studies; D005260:Female; D006403:Hematologic Tests / Q000662:veterinary; D006799:Housing, Animal / Q000145:classification; D008297:Male; D012016:Reference Values; D012737:Sex Factors
keywords: African green monkeys; Chlorocebus aethiops sabaeus; hematology; serum biochemistry