title: Performance characteristics of the new Abbott Real Time MTB assay for detection of Mycobacterium tuberculosis complex in respiratory specimens.
abstract: The performance of the Abbott Real Time MTB assay for detection of Mycobacterium tuberculosis complex in respiratory specimens was evaluated using a standard culture as the reference. The overall concordance between both methods was 0.95. The assay displayed an excellent sensitivity (100% for smear-positive/92.3% for smear-negative specimens) and specificity (100%).
authors.affiliation: Microbiology Service, University Clinic Hospital, Institute of Research INCLIVA, Valencia, Spain.
authors.forename: Víctor
authors.initials: V
authors.lastname: Vinuesa
authors.affiliation: Microbiology Service, University Clinic Hospital, Institute of Research INCLIVA, Valencia, Spain; Department of Microbiology, School of Medicine, University of Valencia, Spain. Electronic address: david.navarro@uv.es.
authors.forename: David
authors.initials: D
authors.lastname: Navarro
authors.affiliation: Microbiology Service, University Clinic Hospital, Institute of Research INCLIVA, Valencia, Spain.
authors.forename: Sandrine
authors.initials: S
authors.lastname: Poujois
authors.affiliation: Microbiology Service, University Clinic Hospital, Institute of Research INCLIVA, Valencia, Spain.
authors.forename: Susana
authors.initials: S
authors.lastname: Zaragoza
authors.affiliation: Microbiology Service, University Clinic Hospital, Institute of Research INCLIVA, Valencia, Spain; Department of Microbiology, School of Medicine, University of Valencia, Spain.
authors.forename: Rafael
authors.initials: R
authors.lastname: Borrás
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000235:genetics*; D011933:Reagent Kits, Diagnostic / Q000592:standards; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods / Q000592:standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis* / Q000382:microbiology*; D055815:Young Adult
chemical_list: D011933:Reagent Kits, Diagnostic
keywords: Acid-fast smear; Culture; Mycobacterium tuberculosis complex; Real-time PCR assays; Respiratory specimens