title: A label-free electrochemical immunosensor for hepatitis B based on hyaluronic acid-carbon nanotube hybrid film.
abstract: An electrochemical immunosensor developed for detection of antibodies to hepatitis B core protein (anti-HBc) is described. Anti-HBc is the earliest serological marker from hepatitis B virus (HBV) infection, remaining all life after contact with virus, being considered the most important marker for uses in screening of blood bank. A nanohybrid surface assembled onto a glassy carbon electrode consisting of amino carbon nanotubes recovered by hyaluronic acid was used as sensing platform to detect the anti-HBc. All the steps of electrode surface modification were characterized by Scanning Electronic Microscopy and extensively evaluated by electrochemical techniques. The electrode response was measured by direct anti-HBc antigen interactions by square wave voltammetry, dispensing uses of label or chemical mediators. Under optimal conditions, the anodic peak current which was proportional to the anti-HBs concentration. The immunosensor response was linear toward anti-HBc in concentrations up to 6 ng mL(-1), with a detection limit of 0.03 ng mL(-1). The linear range achieved was according to clinical level, indicating the immunosensor as promising tool for use as a criterion for blood bag disposal. The enhancement of the hyaluronic acid by carbon nanotube promoted an increase of charge electron transfer, besides a stable platform for HBc.
authors.affiliation: Biomedical Engineering Laboratory, Federal University of Pernambuco, Av. Prof. Moraes Rego, 1235-Cidade Universitária, 50670-901 Recife, Pernambuco, Brazil.
authors.forename: Diego G A
authors.initials: DG
authors.lastname: Cabral
authors.affiliation: Biomedical Engineering Laboratory, Federal University of Pernambuco, Av. Prof. Moraes Rego, 1235-Cidade Universitária, 50670-901 Recife, Pernambuco, Brazil.
authors.forename: Erika C S
authors.initials: EC
authors.lastname: Lima
authors.affiliation: Pathology Department, Pernambuco State University, Recife, Pernambuco, Brazil.
authors.forename: Patrícia
authors.initials: P
authors.lastname: Moura
authors.affiliation: Biomedical Engineering Laboratory, Federal University of Pernambuco, Av. Prof. Moraes Rego, 1235-Cidade Universitária, 50670-901 Recife, Pernambuco, Brazil. Electronic address: rosa.dutra@ufpe.br.
authors.forename: Rosa F
authors.initials: RF
authors.lastname: Dutra
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D055664:Electrochemical Techniques / Q000379:methods*; D006515:Hepatitis B virus / Q000302:isolation & purification*; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry*; D057230:Limit of Detection; D037742:Nanotubes, Carbon / Q000737:chemistry*
chemical_list: D037742:Nanotubes, Carbon; D006820:Hyaluronic Acid
keywords: Carbon nanotubes; Hepatitis B; Hyaluronic acid; Immunosensor; Nanosensor