title: Genetic variation of eggplant mottled dwarf virus from annual and perennial plant hosts.
abstract: The genetic diversity of eggplant mottled dwarf virus (EMDV), a member of the family Rhabdoviridae, was studied using isolates collected from different herbaceous and woody plant species and remote geographic areas. Sequences corresponding to the N, X, P, Y, M and G ORFs as well as the untranslated regions (UTRs) between ORFs were determined from all isolates. Low genetic diversity was found in almost all genomic regions studied except for the X ORF and the UTRs, which were more variable, while interestingly, an EMDV isolate from caper possessed a truncated G gene sequence. Furthermore, low d N /d S ratios, indicative of purifying selection, were calculated for all genes. Phylogenetic analysis showed that the EMDV isolates clustered in three distinct subgroups based on their geographical origin, with the exception of one subgroup that consisted of isolates from northern Greece and Cyprus. Overall, the level of genetic diversity of EMDV differed between seed- and asexually propagated plants in our collection, and this could be related to the mode of transmission.
authors.affiliation: Laboratory of Plant Pathology, School of Agriculture, Faculty of Agriculture, Forestry and Natural Environment, Aristotle University of Thessaloniki, 54 124, Thessaloníki, Greece.
authors.forename: Polyxeni G
authors.initials: PG
authors.lastname: Pappi
authors.affiliation: Laboratory of Plant Pathology, School of Agriculture, Faculty of Agriculture, Forestry and Natural Environment, Aristotle University of Thessaloniki, 54 124, Thessaloníki, Greece. vmaliogk@agro.auth.gr.
authors.forename: Varvara I
authors.initials: VI
authors.lastname: Maliogka
authors.affiliation: Department of Biochemistry and Biotechnology, University of Thessaly, 41 221, Larissa, Greece.
authors.forename: Gregory D
authors.initials: GD
authors.lastname: Amoutzias
authors.affiliation: Laboratory of Plant Pathology, School of Agriculture, Faculty of Agriculture, Forestry and Natural Environment, Aristotle University of Thessaloniki, 54 124, Thessaloníki, Greece.
authors.forename: Nikolaos I
authors.initials: NI
authors.lastname: Katis
mesh_terms: D016000:Cluster Analysis; D003535:Cyprus; D014644:Genetic Variation; D006115:Greece; D019684:Magnoliopsida / Q000821:virology*; D008969:Molecular Sequence Data; D058974:Phylogeography; D010935:Plant Diseases / Q000821:virology*; D012367:RNA, Viral / Q000235:genetics; D012209:Rhabdoviridae / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D012641:Selection, Genetic; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
chemical_list: D012367:RNA, Viral