title: Breakable Hybrid Organosilica Nanocapsules for Protein Delivery.
abstract: The direct delivery of specific proteins to live cells promises a tremendous impact for biological and medical applications, from therapeutics to genetic engineering. However, the process mostly involves tedious techniques and often requires extensive alteration of the protein itself. Herein we report a straightforward approach to encapsulate native proteins by using breakable organosilica matrices that disintegrate upon exposure to a chemical stimulus. The biomolecule-containing capsules were tested for the intracellular delivery of highly cytotoxic proteins into C6 glioma cells. We demonstrate that the shell is broken, the release of the active proteins occurs, and therefore our hybrid architecture is a promising strategy to deliver fragile biomacromolecules into living organisms.
authors.affiliation: Institut de science et d'ingénierie supramoléculaires (ISIS), Université de Strasbourg, 8 Allée Gaspard Monge, 67083, Strasbourg, France. prasetyanto@unistra.fr.
authors.forename: Eko Adi
authors.identifier: http://orcid.org/0000-0002-7641-861X
authors.initials: EA
authors.lastname: Prasetyanto
authors.affiliation: Institut de science et d'ingénierie supramoléculaires (ISIS), Université de Strasbourg, 8 Allée Gaspard Monge, 67083, Strasbourg, France.|Dipartimento di Chimica, Università di Parma, Parma, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Bertucci
authors.affiliation: Institut de science et d'ingénierie supramoléculaires (ISIS), Université de Strasbourg, 8 Allée Gaspard Monge, 67083, Strasbourg, France.
authors.forename: Dedy
authors.initials: D
authors.lastname: Septiadi
authors.affiliation: Dipartimento di Chimica, Università di Parma, Parma, Italy.
authors.forename: Roberto
authors.identifier: http://orcid.org/0000-0002-8026-0923
authors.initials: R
authors.lastname: Corradini
authors.affiliation: Servei de Microscòpia, Universitat Autònoma de Barcelona, Bellaterra, Spain.
authors.forename: Pablo
authors.initials: P
authors.lastname: Castro-Hartmann
authors.affiliation: Institut de science et d'ingénierie supramoléculaires (ISIS), Université de Strasbourg, 8 Allée Gaspard Monge, 67083, Strasbourg, France. decola@unistra.fr.|Institute of Nano Technology (INT), Karlsruhe Institute of Technology, Karlsruhe, Germany. decola@unistra.fr.
authors.forename: Luisa
authors.initials: L
authors.lastname: De Cola
mesh_terms: D046529:Microscopy, Electron, Transmission; D053769:Nanocapsules; D017646:Organosilicon Compounds / Q000008:administration & dosage*; D011506:Proteins / Q000008:administration & dosage*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D053769:Nanocapsules; D017646:Organosilicon Compounds; D011506:Proteins
keywords: encapsulation; hybrid materials; nanostructures; organosilica; protein delivery