title: Efficacy and phytochemical analysis of latex of Calotropis procera against selected dermatophytes.
abstract: BACKGROUND
  Since ancient time, increased interest has been witnessed in the use of an alternative herbal medicine for managing, and the treatment of fungal diseases worldwide. This may be connected to the cost and relative toxicities of the available antifungal drugs. It has been a known tradition practiced in the northern part of Nigeria that parents and teachers use the white latex of Calotropis procera to treat Tinea capitis in children attending the local religious school in the area. This study was conducted in 2009 to ascertain the above claim.
  
  
  METHODS
  Fresh latex of C. procera was screened for their antifungal activity against species of dermatophytes: Trichophyton spp., Microsporum spp. and Epidermophyton spp. using the agar incorporation method.
  
  
  RESULTS
  The result shows that the latex inhibits the in vitro growth of these pathogenic fungi to varying extents with Trichophyton spp. being the most susceptible (P < 0.05) and thus highly inhibited by the latex followed by the Microsporum spp. and Epidermopyton spp. was least inhibited. These inhibitions followed a dose-dependent trend as undiluted latex (100%) gave the highest inhibitory impacts (P < 0.05) when compared to serially diluted latex. The phytochemical analysis of the fresh latex indicated the presence of alkaloids, saponin, tannins, steroids, flavonoids, anthraquinone, and triterpenoids.
  
  
  CONCLUSIONS
  The findings of this study confirmed the perceived usefulness of the latex in the treatment of T. capitis (ringworm) practiced in our society and therefore, its use topically in the treatment of dermatomycotic infection is encouraged.
authors.affiliation: Department of Veterinary Microbiology, Faculty of Veterinary Medicine, Usmanu Danfodiyo University, Sokoto, Nigeria.
authors.forename: Rabiu Muhammad
authors.initials: RM
authors.lastname: Aliyu
authors.affiliation: Department of Veterinary Microbiology, Faculty of Veterinary Medicine, Usmanu Danfodiyo University, Sokoto, Nigeria.
authors.forename: Mikaeel Bala
authors.initials: MB
authors.lastname: Abubakar
authors.affiliation: Department of Natural Sciences, Sokoto State Polytechnic, Sokoto, Nigeria.
authors.forename: Adamu Bello
authors.initials: AB
authors.lastname: Kasarawa
authors.affiliation: Department of Veterinary Microbiology, Faculty of Veterinary Medicine, Usmanu Danfodiyo University, Sokoto, Nigeria.
authors.forename: Yakubu Umar
authors.initials: YU
authors.lastname: Dabai
authors.affiliation: Department of Veterinary Microbiology, Faculty of Veterinary Medicine, Usmanu Danfodiyo University, Sokoto, Nigeria.
authors.forename: Nafiu
authors.initials: N
authors.lastname: Lawal
authors.affiliation: Department of Veterinary Microbiology, Faculty of Veterinary Medicine, Usmanu Danfodiyo University, Sokoto, Nigeria.
authors.forename: Muhammad Bashir
authors.initials: MB
authors.lastname: Bello
authors.affiliation: Department of Microbiology, Faculty of Science, Usmanu Danfodiyo University, Sokoto, Nigeria.
authors.forename: Aminu Yusuf
authors.initials: AY
authors.lastname: Fardami
keywords: Agar incorporation method; antifungal activity; dermatomycotic; latex