title: Does ex vivo CD34+ positive selection influence outcome after autologous hematopoietic stem cell transplantation in systemic sclerosis patients?
abstract: This EBMT Autoimmune Disease Working Party study aimed to evaluate the influence of CD34+ positive graft selection (CD34+) on the outcome of systemic sclerosis (SSc) patients after autologous hematopoietic stem cell transplantation (AHSCT). Clinical and laboratory data from 138 SSc patients at diagnosis, before and after AHSCT were retrospectively analyzed. CD34+ selection was performed in 47.1% (n=65) patients. By multivariate analysis adjusting for all factors differing between the two groups (without or with CD34+), there was no statistically significant difference in terms of overall survival (hazard ratio (HR): 0.98, 95% confidence interval (CI) 0.40-2.39, P=0.96), PFS (HR: 1.55, 95% CI 0.83-2.88, P=0.17) and incidence of relapse or progression (HR: 1.70, 95% CI 0.85-3.38, P=0.13). We demonstrate that CD34+ does not add benefit to the outcome of SSc patient treated with AHSCT. These findings should be further confirmed by prospective randomized trials.
authors.affiliation: Division of Clinical Immunology, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, Brazil.
authors.forename: M C
authors.initials: MC
authors.lastname: Oliveira
authors.affiliation: EBMT, Hôpital Saint Antoine, Paris, France.
authors.forename: M
authors.initials: M
authors.lastname: Labopin
authors.affiliation: Medizinische Universitätsklinik Abt. II, Tübingen, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Henes
authors.affiliation: Department of Haematology, St Vincent's Hospital, Sydney, New South Wales, Australia.
authors.forename: J
authors.initials: J
authors.lastname: Moore
authors.affiliation: Scleroderma Clinic, U.O.C. Day Hospital Rheumatology, Osp. G. Pini, Milan, Italy.
authors.forename: N
authors.initials: N
authors.lastname: Del Papa
authors.affiliation: Assistance Publique-Hôpitaux de Paris, Saint-Louis Hospital, Cell Therapy Unit, Cord Blood Bank and CIC-BT501, Paris, France.|INSERM UMRS 1140, Paris Descartes, Faculté de Pharmacie, Paris, France.
authors.forename: A
authors.initials: A
authors.lastname: Cras
authors.affiliation: Bone Marrow Transplantation Unit, George Papanicolaou General Hospital, Thessaloniki, Greece.
authors.forename: I
authors.initials: I
authors.lastname: Sakellari
authors.affiliation: Ruhr-Universität Bochum, Medizinische Klinik Knappschaftskrankenhaus, Bochum, Germany.
authors.forename: R
authors.initials: R
authors.lastname: Schroers
authors.affiliation: Department of Rheumatology, Leiden University Medical Centre, Leiden, The Netherlands.
authors.forename: H U
authors.initials: HU
authors.lastname: Scherer
authors.affiliation: University of Ferrara-St Anna Hospital, Ferrara, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Cuneo
authors.affiliation: Department of Hematology and Bone Marrow Transplantation, Medical University of Silesia, Katowice, Poland.
authors.forename: S
authors.initials: S
authors.lastname: Kyrcz-Krzemien
authors.affiliation: Department of Rheumatology, University Hospital Basel, Basel, Switzerland.
authors.forename: T
authors.initials: T
authors.lastname: Daikeler
authors.affiliation: Department of Rheumatology and Clinical Immunology, Charité-University Medicine Berlin, Berlin, Germany.
authors.forename: T
authors.initials: T
authors.lastname: Alexander
authors.affiliation: Department of Medicine-Hematology and Oncology, University of Freiburg, Freiburg, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Finke
authors.affiliation: EBMT Paris Office, Hôpital Saint Antoine, Paris, France.
authors.forename: M
authors.initials: M
authors.lastname: Badoglio
authors.affiliation: Division of Hematology, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, Brazil.
authors.forename: B
authors.initials: B
authors.lastname: Simões
authors.affiliation: Department of Hematology, Sheffield Teaching Hospitals NHS Foundation Trust, Sheffield, UK.
authors.forename: J A
authors.initials: JA
authors.lastname: Snowden
authors.affiliation: Paris 7 University, INSERM U1160, Paris, France.|Unité de Médecine Interne et Pathologie Vasculaire, Saint Louis Hospital, Assistance Publique des Hôpitaux de Paris, Paris 7 Denis Diderot University, Paris, France.
authors.forename: D
authors.initials: D
authors.lastname: Farge
mesh_terms: D000293:Adolescent; D000328:Adult; D018952:Antigens, CD34; D064592:Autografts; D018572:Disease-Free Survival; D005260:Female; D005500:Follow-Up Studies; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods*; D006801:Humans; D008297:Male; D008875:Middle Aged; D012595:Scleroderma, Systemic / Q000401:mortality* / Q000628:therapy*; D015996:Survival Rate
chemical_list: D018952:Antigens, CD34