title: Sodium orthovanadate induces the apoptosis of SH-SY5Y cells by inhibiting PIWIL2.
abstract: PIWIs have been shown to be abnormally expressed in a variety of cancers and may be important in the maintenance and invasion of cancer cells. The high expression of PIWIL2 contributed to the resistance effect of cisplatin in colon cancer cells, and the knockout of the PIWIL2 gene reduced the aggressive nature and malignant degree of colon cancer cells. Sodium orthovanadate (SOV) is a vanadium compound, and exhibited antineoplastic activity in certain types of human cancer cells, including lung, kidney and prostate cancer cells. However, its effects in human neuroblastoma (NB) cells have not yet been reported. The objective of this study was to investigate the effect of SOV on the apoptosis of NB cells and to explore how PIWIL2 is involved in the mechanism underlying this effect. In the present study, SH‑SY5Y cells were treated with SOV and the optimal concentration was determined for further assays. Cell apoptosis, cell count, viability, the cell cycle, and the expression of PIWIL2 mRNA and protein were then determined. The results showed that SOV could induce cell apoptosis, reduce the percentage of viable cells, induce accumulation of SH‑SY5Y cells at the G2/M and S phase of the cell cycle, and inhibit the expression of PIWIL2 and Bcl‑2 mRNA and protein. The results suggested that the underlying mechanisms may be, at least in part, due to SOV inhibiting the expression of PIWIL2. These findings demonstrated the effect of SOV and supported its further evaluation as a treatment for human NB.
authors.affiliation: Department of Tissue Engineering, School of Fundamental Science, China Medical University, Shenyang, Liaoning 110122, P.R. China.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Tian
authors.affiliation: Department of Tissue Engineering, School of Fundamental Science, China Medical University, Shenyang, Liaoning 110122, P.R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Fan
authors.affiliation: Department of Tissue Engineering, School of Fundamental Science, China Medical University, Shenyang, Liaoning 110122, P.R. China.
authors.forename: Weijian
authors.initials: W
authors.lastname: Hou
authors.affiliation: Department of Tissue Engineering, School of Fundamental Science, China Medical University, Shenyang, Liaoning 110122, P.R. China.
authors.forename: Shuling
authors.initials: S
authors.lastname: Bai
authors.affiliation: Department of Tissue Engineering, School of Fundamental Science, China Medical University, Shenyang, Liaoning 110122, P.R. China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Ao
authors.affiliation: Department of Tissue Engineering, School of Fundamental Science, China Medical University, Shenyang, Liaoning 110122, P.R. China.
authors.forename: Hao
authors.initials: H
authors.lastname: Tong
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D060565:Argonaute Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D002452:Cell Count; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D016195:G2 Phase / Q000187:drug effects; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D008938:Mitosis / Q000187:drug effects; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014638:Vanadates / Q000494:pharmacology*
chemical_list: D060565:Argonaute Proteins; C486868:PIWIL2 protein, human; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; D014638:Vanadates