title: Polyelectrolytes Multilayers to Modulate Cell Adhesion: A Study of the Influence of Film Composition and Polyelectrolyte Interdigitation on the Adhesion of the A549 Cell Line.
abstract: Polyelectrolyte multilayers (PEMs) with different polycation/polyanion pairs are fabricated by the layer-by-layer technique employing synthetic, natural, and both types of polyelectrolytes. The impact of the chemical composition of PEMs on cell adhesion is assessed by studying cell shape, spreading area, focal contacts, and cell proliferation for the A549 cell line. Cells exhibit good adhesion on PEMs containing natural polycations and poly(sodium 4-styrenesulfonate) (PSS) as polyanion, but limited adhesion is observed on PEMs fabricated from both natural polyelectrolytes. PEMs are then assembled, depositing a block of natural polyelectrolytes on top of a stiffer block with PSS as polyanion. Cell adhesion is enhanced on top of the diblock PEMs compared to purely natural PEMs. This fact could be explained by the interdigitation between polyelectrolytes from the two blocks. Diblock PEM assembly provides a simple means to tune cell adhesion on biocompatible PEMs.
authors.affiliation: Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas (INIFTA), (UNLP, CONICET), Sucursal 4, Casilla de Correo 16, 1900, La Plata, Argentina.
authors.forename: Nicolás E
authors.initials: NE
authors.lastname: Muzzio
authors.affiliation: Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas (INIFTA), (UNLP, CONICET), Sucursal 4, Casilla de Correo 16, 1900, La Plata, Argentina.
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Pasquale
authors.affiliation: Soft Matter Nanotechnology Group, CIC biomaGUNE, Paseo Marimón 182 C, 20009, San Sebastián, Gipuzkoa, Spain.
authors.forename: Danijela
authors.initials: D
authors.lastname: Gregurec
authors.affiliation: Soft Matter Nanotechnology Group, CIC biomaGUNE, Paseo Marimón 182 C, 20009, San Sebastián, Gipuzkoa, Spain.
authors.forename: Eleftheria
authors.initials: E
authors.lastname: Diamanti
authors.affiliation: Soft Matter Nanotechnology Group, CIC biomaGUNE, Paseo Marimón 182 C, 20009, San Sebastián, Gipuzkoa, Spain.
authors.forename: Marija
authors.initials: M
authors.lastname: Kosutic
authors.affiliation: Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas (INIFTA), (UNLP, CONICET), Sucursal 4, Casilla de Correo 16, 1900, La Plata, Argentina.
authors.forename: Omar
authors.initials: O
authors.lastname: Azzaroni
authors.affiliation: Soft Matter Nanotechnology Group, CIC biomaGUNE, Paseo Marimón 182 C, 20009, San Sebastián, Gipuzkoa, Spain.
authors.forename: Sergio E
authors.initials: SE
authors.lastname: Moya
mesh_terms: D000072283:A549 Cells; D000180:Acrylic Resins / Q000737:chemistry / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects; D022001:Focal Adhesions / Q000187:drug effects / Q000502:physiology; D006801:Humans; D011094:Polyethyleneimine / Q000737:chemistry / Q000494:pharmacology; D011095:Polyethylenes / Q000737:chemistry / Q000494:pharmacology*; D011107:Polylysine / Q000737:chemistry / Q000494:pharmacology*; D011108:Polymers / Q000737:chemistry / Q000494:pharmacology*; D000644:Quaternary Ammonium Compounds / Q000737:chemistry / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D013451:Sulfonic Acids / Q000737:chemistry / Q000494:pharmacology*; D013499:Surface Properties
chemical_list: D000180:Acrylic Resins; D011095:Polyethylenes; D011108:Polymers; D000644:Quaternary Ammonium Compounds; D013451:Sulfonic Acids; D011107:Polylysine; C041004:poly-N,N-dimethyl-N,N-diallylammonium chloride; C006903:carbopol 940; D011094:Polyethyleneimine; C077114:styrenesulfonic acid polymer
keywords: adhesion; biocompatibility; mechanical properties; polyelectrolytes; tissue engineering