title: Allergic sensitization modifies the pulmonary expression of 5-hydroxytryptamine receptors in guinea pigs.
abstract: There is mounting evidence that 5-hydroxytryptamine (5-HT) plays a role in asthma. However, scarce information exists about the pulmonary expression of 5-HT receptors and its modification after allergic sensitization. In the present work, we explored the expression of 5-HT1A, 5-HT2A, 5-HT3, 5-HT4, 5-ht5a, 5-HT6, and 5-HT7 receptors in lungs from control and sensitized guinea pigs through qPCR and Western blot. In control animals, mRNA from all receptors was detectable in lung homogenates, especially from 5-HT2A and 5-HT4 receptors. Sensitized animals had decreased mRNA expression of 5-HT2A and 5-HT4 receptors and increased that of 5-HT7 receptor. In contrast, they had increased protein expression of 5-HT2A receptor in bronchial epithelium and of 5-HT4 receptor in lung parenchyma. The degree of airway response to the allergic challenge was inversely correlated with mRNA expression of the 5-HT1A receptor. In summary, our results showed that major 5-HT receptor subtypes are constitutively expressed in the guinea pig lung, and that allergic sensitization modifies the expression of 5-HT2A, 5-HT4, and 5-HT7 receptors.
authors.affiliation: Departamento de Investigación en Hiperreactividad Bronquial, Instituto Nacional de Enfermedades Respiratorias, México DF, Mexico.
authors.forename: Guadalupe
authors.initials: G
authors.lastname: Córdoba-Rodríguez
authors.affiliation: Departamento de Investigación en Hiperreactividad Bronquial, Instituto Nacional de Enfermedades Respiratorias, México DF, Mexico.
authors.forename: Mario H
authors.initials: MH
authors.lastname: Vargas
authors.affiliation: Laboratorio de Biología Molecular, Departamento de Investigación en Fibrosis Pulmonar, Instituto Nacional de Enfermedades Respiratorias, México DF, Mexico.
authors.forename: Víctor
authors.initials: V
authors.lastname: Ruiz
authors.affiliation: Departamento de Investigación en Hiperreactividad Bronquial, Instituto Nacional de Enfermedades Respiratorias, México DF, Mexico.
authors.forename: Verónica
authors.initials: V
authors.lastname: Carbajal
authors.affiliation: Unidad de Investigación Médica en Enfermedades Neurológicas, Hospital de Especialidades, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, México DF, Mexico.
authors.forename: Patricia
authors.initials: P
authors.lastname: Campos-Bedolla
authors.affiliation: Instituto Tecnológico y de Estudios Superiores de Monterrey, Campus Ciudad de México, México DF, Mexico.
authors.forename: Paulina
authors.initials: P
authors.lastname: Mercadillo-Herrera
authors.affiliation: Departamento de Investigación en Hiperreactividad Bronquial, Instituto Nacional de Enfermedades Respiratorias, México DF, Mexico.
authors.forename: José Luis
authors.initials: JL
authors.lastname: Arreola-Ramírez
authors.affiliation: Departamento de Investigación en Hiperreactividad Bronquial, Instituto Nacional de Enfermedades Respiratorias, México DF, Mexico. Electronic address: psegura@unam.mx.
authors.forename: Patricia
authors.initials: P
authors.lastname: Segura-Medina
mesh_terms: D000818:Animals; D001249:Asthma / Q000378:metabolism*; D004195:Disease Models, Animal; D006168:Guinea Pigs; D006967:Hypersensitivity / Q000378:metabolism*; D007114:Immunization; D008297:Male; D044402:Receptor, Serotonin, 5-HT2A / Q000378:metabolism; D011985:Receptors, Serotonin / Q000378:metabolism*; D044422:Receptors, Serotonin, 5-HT4 / Q000378:metabolism
chemical_list: D044402:Receptor, Serotonin, 5-HT2A; D011985:Receptors, Serotonin; C104425:serotonin 7 receptor; D044422:Receptors, Serotonin, 5-HT4
keywords: 5-HT receptors; Allergic sensitization; Asthma model; Guinea pig; Serotonin receptors