title: Multi-omic profiles of human non-alcoholic fatty liver disease tissue highlight heterogenic phenotypes.
abstract: Non-alcoholic fatty liver disease (NAFLD) is a consequence of sedentary life style and high fat diets with an estimated prevalence of about 30% in western countries. It is associated with insulin resistance, obesity, glucose intolerance and drug toxicity. Additionally, polymorphisms within, e.g., APOC3, PNPLA3, NCAN, TM6SF2 and PPP1R3B, correlate with NAFLD. Several studies have already investigated later stages of the disease. This study explores the early steatosis stage of NAFLD with the aim of identifying molecular mechanisms underlying the etiology of NAFLD. We analyzed liver biopsies and serum samples from patients with high- and low-grade steatosis (also pre-disease states) employing transcriptomics, ELISA-based serum protein analyses and metabolomics. Here, we provide a detailed description of the various related datasets produced in the course of this study. These datasets may help other researchers find new clues for the etiology of NAFLD and the mechanisms underlying its progression to more severe disease states.
authors.affiliation: Medical Faculty, Institute for Stem Cell Research and Regenerative Medicine, Heinrich Heine University, 40225 Düsseldorf, Germany.
authors.forename: Wasco
authors.initials: W
authors.lastname: Wruck
authors.affiliation: Institute of Pathology, Medical University of Graz, Graz 8036, Austria.
authors.forename: Karl
authors.initials: K
authors.lastname: Kashofer
authors.affiliation: The Manchester Centre for Integrative Systems Biology, Manchester Institute of Biotechnology, University of Manchester, Manchester M1 7DN, UK.
authors.forename: Samrina
authors.initials: S
authors.lastname: Rehman
authors.affiliation: Department of Vertebrate Genomics, Max Planck Institute for Molecular Genetics, Ihnestrasse 63, Berlin 14195, Germany.
authors.forename: Andriani
authors.initials: A
authors.lastname: Daskalaki
authors.affiliation: Division of Molecular Genome Analysis, German Cancer Research Center (DKFZ), 69120 Heidelberg, Germany.
authors.forename: Daniela
authors.initials: D
authors.lastname: Berg
authors.affiliation: Magnetic Resonance Center (CERM), University of Florence, 50019 Florence, Italy.
authors.forename: Ewa
authors.initials: E
authors.lastname: Gralka
authors.affiliation: Department of Vertebrate Genomics, Max Planck Institute for Molecular Genetics, Ihnestrasse 63, Berlin 14195, Germany.
authors.forename: Justyna
authors.initials: J
authors.lastname: Jozefczuk
authors.affiliation: Department of Vertebrate Genomics, Max Planck Institute for Molecular Genetics, Ihnestrasse 63, Berlin 14195, Germany.
authors.forename: Katharina
authors.initials: K
authors.lastname: Drews
authors.affiliation: Department of Vertebrate Genomics, Max Planck Institute for Molecular Genetics, Ihnestrasse 63, Berlin 14195, Germany.
authors.forename: Vikash
authors.initials: V
authors.lastname: Pandey
authors.affiliation: Institute for Pathology &Comprehensive Cancer Center, Cancer Stem Cell Group, Charité-Universitätsmedizin, 10117 Berlin, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Regenbrecht
authors.affiliation: Department of Vertebrate Genomics, Max Planck Institute for Molecular Genetics, Ihnestrasse 63, Berlin 14195, Germany.
authors.forename: Christoph
authors.initials: C
authors.lastname: Wierling
authors.affiliation: Magnetic Resonance Center (CERM), University of Florence, 50019 Florence, Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Turano
authors.affiliation: Division of Molecular Genome Analysis, German Cancer Research Center (DKFZ), 69120 Heidelberg, Germany.
authors.forename: Ulrike
authors.initials: U
authors.lastname: Korf
authors.affiliation: Institute of Pathology, Medical University of Graz, Graz 8036, Austria.
authors.forename: Kurt
authors.initials: K
authors.lastname: Zatloukal
authors.affiliation: Department of Vertebrate Genomics, Max Planck Institute for Molecular Genetics, Ihnestrasse 63, Berlin 14195, Germany.
authors.forename: Hans
authors.initials: H
authors.lastname: Lehrach
authors.affiliation: The Manchester Centre for Integrative Systems Biology, Manchester Institute of Biotechnology, University of Manchester, Manchester M1 7DN, UK.|Netherlands Institute for Systems Biology, VU University Amsterdam, HV NL-1081 Amsterdam, The Netherlands.|Synthetic Systems Biology, Swammerdam Institute for Life Sciences, University of Amsterdam, 1018 WS Amsterdam, The Netherlands.
authors.forename: Hans V
authors.initials: HV
authors.lastname: Westerhoff
authors.affiliation: Medical Faculty, Institute for Stem Cell Research and Regenerative Medicine, Heinrich Heine University, 40225 Düsseldorf, Germany.|Department of Vertebrate Genomics, Max Planck Institute for Molecular Genetics, Ihnestrasse 63, Berlin 14195, Germany.
authors.forename: James
authors.initials: J
authors.lastname: Adjaye
mesh_terms: D053305:Apolipoprotein C-III / Q000235:genetics; D001706:Biopsy; D011508:Chondroitin Sulfate Proteoglycans / Q000235:genetics; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006801:Humans; D037181:Lectins, C-Type / Q000235:genetics; D008049:Lipase / Q000235:genetics; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008565:Membrane Proteins / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000235:genetics; D058580:Neurocan; D065626:Non-alcoholic Fatty Liver Disease / Q000209:etiology / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D054645:Protein Phosphatase 1 / Q000235:genetics; D000217:Acyltransferases; D054512:Phospholipases A2, Calcium-Independent
chemical_list: D053305:Apolipoprotein C-III; D011508:Chondroitin Sulfate Proteoglycans; D037181:Lectins, C-Type; D008049:Lipase; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D058580:Neurocan; D054645:Protein Phosphatase 1; C419195:TM6SF2 protein, human; C076817:NCAN protein, human; C533682:PNPLA3 protein, human; D000217:Acyltransferases; D054512:Phospholipases A2, Calcium-Independent; C586823:PPP1R3B protein, human