title: Cell surface heparan sulfate proteoglycans are involved in the binding of Hsp90α and Hsp90β to the cell plasma membrane.
abstract: Extracellular membrane-bound and secreted heat shock protein 90 (Hsp90) is known to be involved in cell motility and invasion. The mechanism of Hsp90 anchoring to the plasma membrane remains obscure. We showed that treatment of human glioblastoma A-172 and fibrosarcoma HT1080 cells with sodium chlorate, heparinase, and heparin causes a prominent loss of 2 Hsp90 cytosolic isoforms, Hsp90α and Hsp90β, from the cell surface and strongly inhibits the binding of exogenous Hsp90 to cells. We revealed that Hsp90α and Hsp90β are partly colocalized with heparan sulfate proteoglycans (HSPGs) on the cell surface and that this colocalization was sensitive to heparin. The results demonstrate that cell surface HSPGs are involved in the binding/anchoring of Hsp90α and Hsp90β to the plasma membrane.
authors.affiliation: a Institute of Cell Biophysics; Russian Academy of Sciences ; Pushchino , Russia.
authors.forename: Anastasiya V
authors.initials: AV
authors.lastname: Snigireva
authors.affiliation: a Institute of Cell Biophysics; Russian Academy of Sciences ; Pushchino , Russia.
authors.forename: Veronika V
authors.initials: VV
authors.lastname: Vrublevskaya
authors.affiliation: a Institute of Cell Biophysics; Russian Academy of Sciences ; Pushchino , Russia.
authors.forename: Vladimir N
authors.initials: VN
authors.lastname: Afanasyev
authors.affiliation: a Institute of Cell Biophysics; Russian Academy of Sciences ; Pushchino , Russia.|b Pushchino State Institute of Life Sciences ; Pushchino , Russia.
authors.forename: Oleg S
authors.initials: OS
authors.lastname: Morenkov
mesh_terms: D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism; D002465:Cell Movement / Q000235:genetics; D005354:Fibrosarcoma / Q000378:metabolism* / Q000473:pathology; D018841:HSP90 Heat-Shock Proteins / Q000378:metabolism*; D006493:Heparin / Q000494:pharmacology; D006497:Heparan Sulfate / Q000378:metabolism*; D006801:Humans; D008562:Membrane Glycoproteins / Q000378:metabolism; D011485:Protein Binding
chemical_list: D018841:HSP90 Heat-Shock Proteins; D006493:Heparin; D006497:Heparan Sulfate; D008562:Membrane Glycoproteins; C533585:HSP90AA2P protein, human; C493165:HSP90AB1 protein, human
keywords: anchoring and binding to plasma membrane; and Hsp90β; cell surface HSPGs; cell surface-bound Hsp90α; extracellular Hsp90; human glioblastoma A-172 and fibrosarcoma HT1080 cells