title: EGCG/gelatin-doxorubicin gold nanoparticles enhance therapeutic efficacy of doxorubicin for prostate cancer treatment.
abstract: OBJECTIVE
  Development of epigallocatechin gallate (EGCG) and gelatin-doxorubicin conjugate (GLT-DOX)-coated gold nanoparticles (DOX-GLT/EGCG AuNPs) for fluorescence imaging and inhibition of prostate cancer cell growth.
  
  
  METHODS
  AuNPs alternatively coated with EGCG and DOX-GLT conjugates were prepared by a layer-by-layer assembly method. The physicochemical properties of the AuNPs and the effect of Laminin 67R receptor-mediated endocytosis on the anticancer efficacy of the AuNPs were examined.
  
  
  RESULTS
  The AuNPs significantly inhibit the proliferation of PC-3 cancer cell and the enzyme-responsive intracellular release of DOX could be tracked by monitoring the recovery of the fluorescence signal of DOX.
  
  
  CONCLUSIONS
  Laminin 67R receptor-mediated delivery of DOX using the AuNPs enhanced cellular uptake of DOX and improved apoptosis of PC-3 cells.
authors.affiliation: Institute of Organic & Polymeric Materials, National Taipei University of Technology, Taipei 10608, Taiwan.
authors.forename: Li-Chu
authors.initials: LC
authors.lastname: Tsai
authors.affiliation: Institute of Organic & Polymeric Materials, National Taipei University of Technology, Taipei 10608, Taiwan.|Institute of Biomedical Engineering, National Taiwan University, Taipei 10002, Taiwan.
authors.forename: Hao-Ying
authors.initials: HY
authors.lastname: Hsieh
authors.affiliation: Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei 11031, Taiwan.
authors.forename: Kun-Ying
authors.initials: KY
authors.lastname: Lu
authors.affiliation: Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei 11031, Taiwan.
authors.forename: Sin-Yu
authors.initials: SY
authors.lastname: Wang
authors.affiliation: Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei 11031, Taiwan.|Department of Biochemistry & Molecular Cell Biology, School of Medicine, College of Medicine, Taipei Medical University, Taipei City 11031, Taiwan.|Graduate Institute of Nanomedicine & Medical Engineering, College of Biomedical Engineering, Taipei Medical University, Taipei 11031, Taiwan.
authors.forename: Fwu-Long
authors.initials: FL
authors.lastname: Mi
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000008:administration & dosage* / Q000737:chemistry; D002392:Catechin / Q000031:analogs & derivatives* / Q000737:chemistry; D045744:Cell Line, Tumor; D004317:Doxorubicin / Q000008:administration & dosage* / Q000737:chemistry; D005780:Gelatin / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D006801:Humans; D008297:Male; D053768:Metal Nanoparticles
chemical_list: D000903:Antibiotics, Antineoplastic; D006046:Gold; D004317:Doxorubicin; D002392:Catechin; D005780:Gelatin; C045651:epigallocatechin gallate
keywords: EGCG; Lam 67R; MMP-2; cellular uptake; doxorubicin; drug release; gelatin; gold nanoparticles; prostate cancer