title: The Effect of Green Tea Extract on Reproductive Improvement in Estradiol Valerate-Induced Polycystic Ovarian Syndrome in Rat.
abstract: Polycystic ovary syndrome (PCOS) is a reproductive and metabolic disorder in which the level of oxidative elements in blood rises. Green tea is a potent antioxidant since it contains catechins. In this study, the effect of hydro-alcoholic green tea extract on PCOS rats was examined. For PCOS induction, 96 mature Wistar rats were given estradiol valerate. After 60 days, the rats were divided into four groups including PCOS group and three experimental groups, which were given 50, 100 and 200 mg/Kg BW green tea extract 10 days, intraperitoneally. The serum concentration level of FSH, LH, testosterone and insulin were measured using ELISA method, while the serum concentration level of glucose was measured using glucose oxidase methods. Insulin resistance was calculated using HOMA-IR formulation. The data were analyzed using the one-Way ANOVA method considering P<0/05 level of significance. There were a significant reduction in LH serum level, body and ovarian weight between the green tea extract treated-groups compare to PCOS. Moreover, a significant reduction in insulin resistance index was seen in the treatment groups related to PCOS. Histomorphometric studies also showed the significant changes in the number of follicles and theca layer thickness. These changes demonstrated a marked improvement in the symptoms of PCOS which may be due to green tea effects on oxidative stress pathways. Green tea can be considered as a potentially effective drug for treatment of PCOS, Insulin resistance and Type II diabetes.
authors.affiliation: Department of Cell and Molecular Biology, School of Biological Sciences, Kharazmi University, Tehran, Iran.
authors.forename: Habibeh
authors.initials: H
authors.lastname: Ghafurniyan
authors.affiliation: Department of Cell and Molecular Biology, School of Biological Sciences, Kharazmi University, Tehran, Iran.
authors.forename: Mahnaz
authors.initials: M
authors.lastname: Azarnia
authors.affiliation: Department of Cell and Molecular Biology, School of Biological Sciences, Kharazmi University, Tehran, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Nabiuni
authors.affiliation: Department of Cell and Molecular Biology, School of Biological Sciences, Kharazmi University, Tehran, Iran.
authors.forename: Latifeh
authors.initials: L
authors.lastname: Karimzadeh
keywords: Green tea extract; Insulin resistance; Polycystic ovary syndrome; Wistar rat