title: Current advances in cell electrophysiology: applications for the analysis of intercellular communications within the neurovascular unit.
abstract: Patch clamp is a golden standard for studying (patho)physiological processes affecting membranes of excitable cells. This method is rather labor-intensive and requires well-trained professionals and long-lasting experimental procedures; therefore, accurate designing of the experiments with patch clamp methodology as well as collecting and analyzing the data obtained are essential for the widely spread implementation of this method into the routine research practice. Recently, the method became very prospective not only for the characterization of single excitable cells but also for the detailed assessment of intercellular communication, i.e. within the neurovascular unit. Here, we analyze the main advantages and disadvantages of patch clamp method, with special focus on the tendencies in clamping technique improvement with the help of patch electrodes for the assessment of intercellular communication in the brain.
authors.forename: Anton N
authors.initials: AN
authors.lastname: Shuvaev
authors.forename: Vladimir V
authors.initials: VV
authors.lastname: Salmin
authors.forename: Natalia V
authors.initials: NV
authors.lastname: Kuvacheva
authors.forename: Elena A
authors.initials: EA
authors.lastname: Pozhilenkova
authors.forename: Andrey V
authors.initials: AV
authors.lastname: Morgun
authors.forename: Olga L
authors.initials: OL
authors.lastname: Lopatina
authors.forename: Alla B
authors.initials: AB
authors.lastname: Salmina
authors.forename: Sergey N
authors.initials: SN
authors.lastname: Illarioshkin
mesh_terms: D000200:Action Potentials / Q000502:physiology; D000818:Animals; D001921:Brain / Q000502:physiology*; D002450:Cell Communication / Q000502:physiology*; D004594:Electrophysiology / Q000379:methods; D006801:Humans; D000069336:Neurovascular Coupling / Q000502:physiology*; D018408:Patch-Clamp Techniques / Q000379:methods