title: Antibacterial Peptide-Based Gel for Prevention of Medical Implanted-Device Infection.
abstract: Implanted medical devices are prone to infection. Designing new strategies to reduce infection and implant rejection are an important challenge for modern medicine. To this end, in the last few years many hydrogels have been designed as matrices for antimicrobial molecules destined to fight frequent infection found in moist environments like the oral cavity. In this study, two types of original hydrogels containing the antimicrobial peptide Cateslytin have been designed. The first hydrogel is based on alginate modified with catechol moieties (AC gel). The choice of these catechol functional groups which derive from mussel's catechol originates from their strong adhesion properties on various surfaces. The second type of gel we tested is a mixture of alginate catechol and thiol-terminated Pluronic (AC/PlubisSH), a polymer derived from Pluronic, a well-known biocompatible polymer. This PlubisSH polymer has been chosen for its capacity to enhance the cohesion of the composition. These two gels offer new clinical uses, as they can be injected and jellify in a few minutes. Moreover, we show these gels strongly adhere to implant surfaces and gingiva. Once gelled, they demonstrate a high level of rheological properties and stability. In particular, the dissipative energy of the (AC/PlubisSH) gel detachment reaches a high value on gingiva (10 J.m-2) and on titanium alloys (4 J.m-2), conferring a strong mechanical barrier. Moreover, the Cateslytin peptide in hydrogels exhibited potent antimicrobial activities against P. gingivalis, where a strong inhibition of bacterial metabolic activity and viability was observed, indicating reduced virulence. Gel biocompatibility tests indicate no signs of toxicity. In conclusion, these new hydrogels could be ideal candidates in the prevention and/or management of periimplant diseases.
authors.affiliation: Institut National de la Santé Et de la Recherche Médicale, Unité Mixte de Recherche-S 1121, Biomatériaux et Bioingénierie, Strasbourg, France.|Faculté de Chirurgie Dentaire, Université de Strasbourg, Strasbourg, France.
authors.forename: Mihaela
authors.initials: M
authors.lastname: Mateescu
authors.affiliation: Institut National de la Santé Et de la Recherche Médicale, Unité Mixte de Recherche-S 1121, Biomatériaux et Bioingénierie, Strasbourg, France.|Faculté de Chirurgie Dentaire, Université de Strasbourg, Strasbourg, France.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Baixe
authors.affiliation: Institut Charles Sadron, Centre National de la Recherche Scientifique, Université de Strasbourg, Strasbourg, France.
authors.forename: Tony
authors.initials: T
authors.lastname: Garnier
authors.affiliation: Institut Charles Sadron, Centre National de la Recherche Scientifique, Université de Strasbourg, Strasbourg, France.
authors.forename: Loic
authors.initials: L
authors.lastname: Jierry
authors.affiliation: Institut National de la Santé Et de la Recherche Médicale, Unité Mixte de Recherche-S 1121, Biomatériaux et Bioingénierie, Strasbourg, France.|Faculté de Chirurgie Dentaire, Université de Strasbourg, Strasbourg, France.
authors.forename: Vincent
authors.initials: V
authors.lastname: Ball
authors.affiliation: Institut National de la Santé Et de la Recherche Médicale, Unité Mixte de Recherche-S 1121, Biomatériaux et Bioingénierie, Strasbourg, France.|Faculté de Chirurgie Dentaire, Université de Strasbourg, Strasbourg, France.
authors.forename: Youssef
authors.initials: Y
authors.lastname: Haikel
authors.affiliation: Institut National de la Santé Et de la Recherche Médicale, Unité Mixte de Recherche-S 1121, Biomatériaux et Bioingénierie, Strasbourg, France.|Faculté de Chirurgie Dentaire, Université de Strasbourg, Strasbourg, France.
authors.forename: Marie Hélène
authors.initials: MH
authors.lastname: Metz-Boutigue
authors.affiliation: Institut de Science des Matériaux de Mulhouse, Centre National de la Recherche Scientifique LRC 7228, Mulhouse, France.
authors.forename: Michel
authors.initials: M
authors.lastname: Nardin
authors.affiliation: Institut National de la Santé Et de la Recherche Médicale, Unité Mixte de Recherche-S 1121, Biomatériaux et Bioingénierie, Strasbourg, France.|Faculté de Chirurgie Dentaire, Université de Strasbourg, Strasbourg, France.|Institut Charles Sadron, Centre National de la Recherche Scientifique, Université de Strasbourg, Strasbourg, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Schaaf
authors.affiliation: Institut National de la Santé Et de la Recherche Médicale, Unité Mixte de Recherche-S 1121, Biomatériaux et Bioingénierie, Strasbourg, France.|Faculté de Chirurgie Dentaire, Université de Strasbourg, Strasbourg, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Etienne
authors.affiliation: Institut National de la Santé Et de la Recherche Médicale, Unité Mixte de Recherche-S 1121, Biomatériaux et Bioingénierie, Strasbourg, France.|Faculté de Chirurgie Dentaire, Université de Strasbourg, Strasbourg, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Lavalle
mesh_terms: D000464:Alginates / Q000737:chemistry; D000497:Alloys / Q000737:chemistry; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry* / Q000494:pharmacology; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D002396:Catechols / Q000737:chemistry; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D053379:Chromogranin A / Q000737:chemistry / Q000494:pharmacology; D015921:Dental Implants / Q000382:microbiology; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D010446:Peptide Fragments / Q000737:chemistry / Q000494:pharmacology; D020442:Poloxamer / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D016966:Porphyromonas gingivalis / Q000187:drug effects; D012212:Rheology
chemical_list: D000464:Alginates; D000497:Alloys; D023181:Antimicrobial Cationic Peptides; D001672:Biocompatible Materials; D002396:Catechols; D053379:Chromogranin A; D015921:Dental Implants; D006603:Hexuronic Acids; D010446:Peptide Fragments; D011108:Polymers; C527456:chromogranin A (344-358); D020442:Poloxamer; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D020723:Glucuronic Acid; C034221:catechol