title: Immobilization of cationic antimicrobial peptides and natural cashew gum in nanosheet systems for the investigation of anti-leishmanial activity.
abstract: This report details the development of thin films containing an antimicrobial peptide, specifically, dermaseptin 01 (GLWSTIKQKGKEAAIAAA-KAAGQAALGAL-NH2, [DRS 01]), and a natural polysaccharide, for a novel application in detecting the presence of Leishmania cells and maintaining anti-leishmanial activity. The peptide DRS 01 was immobilized in conjunction with natural cashew gum (CG) onto an indium tin oxide (ITO) substrate using the Layer-by-Layer (LbL) deposition technique. The LbL film ITO/CG/DRS 01, containing DRS 01 as the outer layer, was capable of detecting the presence of Leishmania cells and acting as an anti-leishmanial system. Detection was performed using cyclic voltammetry (CV) in phosphate buffer (pH7.2) in the presence of promastigote cells (0-10(7)cells/mL). The results showed a linear and inversely proportional relation between the concentration of Leishmania infantum protozoan cells and the measured current values obtained for the films, which was attributed to the effect of peptide-induced lysis of the cell membrane, and resulted in freed residues that were adsorbed on the electrode surface. With this, the paper shows a method using thin films with this new material to demonstrate the anti-leishmanial activity in vitro models of carpet-like mechanisms.
authors.affiliation: Núcleo de Pesquisa em Biodiversidade e Biotecnologia, BIOTEC, Campus Ministro Reis Velloso, CMRV, Universidade Federal do Piauí, UFPI, Parnaíba, PI 64202020, Brazil.
authors.forename: Clicia Ramos
authors.initials: CR
authors.lastname: Bittencourt
authors.affiliation: Núcleo de Pesquisa em Biodiversidade e Biotecnologia, BIOTEC, Campus Ministro Reis Velloso, CMRV, Universidade Federal do Piauí, UFPI, Parnaíba, PI 64202020, Brazil.
authors.forename: Emanuel Airton
authors.initials: EA
authors.lastname: de Oliveira Farias
authors.affiliation: Núcleo de Pesquisa em Biodiversidade e Biotecnologia, BIOTEC, Campus Ministro Reis Velloso, CMRV, Universidade Federal do Piauí, UFPI, Parnaíba, PI 64202020, Brazil.
authors.forename: Karla Costa
authors.initials: KC
authors.lastname: Bezerra
authors.affiliation: Núcleo de Pesquisa em Biodiversidade e Biotecnologia, BIOTEC, Campus Ministro Reis Velloso, CMRV, Universidade Federal do Piauí, UFPI, Parnaíba, PI 64202020, Brazil.
authors.forename: Leiz Maria Costa
authors.initials: LMC
authors.lastname: Véras
authors.affiliation: Núcleo de Pesquisa em Biodiversidade e Biotecnologia, BIOTEC, Campus Ministro Reis Velloso, CMRV, Universidade Federal do Piauí, UFPI, Parnaíba, PI 64202020, Brazil; Laboratório de Pesquisas em Leishmanioses, Instituto de Doenças Tropicais Natan Portela-IDTNP, Teresina 64001450, Brazil.
authors.forename: Vladimir Costa
authors.initials: VC
authors.lastname: Silva
authors.affiliation: Laboratório de Pesquisas em Leishmanioses, Instituto de Doenças Tropicais Natan Portela-IDTNP, Teresina 64001450, Brazil.
authors.forename: Carlos Henrique Nery
authors.initials: CHN
authors.lastname: Costa
authors.affiliation: EMBRAPA Recursos Genéticos e Biotecnologia, 70770-917 Brasília, DF, Brazil; Laboratório de Espectrometria de Massa, LEM, Sala de Nanotecnologia, EMBRAPA, Recursos Genéticos e Biotecnologia, Brasília, DF 70770-917, Brazil.
authors.forename: Marcelo P
authors.initials: MP
authors.lastname: Bemquerer
authors.affiliation: EMBRAPA Recursos Genéticos e Biotecnologia, 70770-917 Brasília, DF, Brazil.
authors.forename: Luciano Paulino
authors.initials: LP
authors.lastname: Silva
authors.affiliation: Núcleo de Pesquisa em Biodiversidade e Biotecnologia, BIOTEC, Campus Ministro Reis Velloso, CMRV, Universidade Federal do Piauí, UFPI, Parnaíba, PI 64202020, Brazil.
authors.forename: José Roberto de
authors.initials: JR
authors.lastname: Souza de Almeida Leite
authors.affiliation: Núcleo de Pesquisa em Biodiversidade e Biotecnologia, BIOTEC, Campus Ministro Reis Velloso, CMRV, Universidade Federal do Piauí, UFPI, Parnaíba, PI 64202020, Brazil; Laboratório Interdisciplinar de Materiais Avançados, LIMAV, CCN, UFPI, Teresina, PI 64049-550, Brazil. Electronic address: carla.eiras.ufpi@gmail.com.
authors.forename: Carla
authors.initials: C
authors.lastname: Eiras
mesh_terms: D031021:Anacardium / Q000737:chemistry*; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry / Q000494:pharmacology*; D055664:Electrochemical Techniques / Q000295:instrumentation*; D004867:Equipment Design; D055767:Immobilized Proteins / Q000737:chemistry / Q000494:pharmacology; D018314:Leishmania infantum / Q000187:drug effects*; D049329:Nanostructures / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D014344:Trypanocidal Agents / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D023181:Antimicrobial Cationic Peptides; D055767:Immobilized Proteins; D010936:Plant Extracts; D014344:Trypanocidal Agents
keywords: Antimicrobial peptides; Cashew gum; Electrochemical device; Layer-by-layer films; Leishmania infantum