title: Analysis of Pirlimycin Residues in Beef Muscle, Milk, and Honey by a Biotin-Streptavidin-Amplified Enzyme-Linked Immunosorbent Assay.
abstract: Food contamination by veterinary drug residues is a worldwide public health concern and requires continuous monitoring. In this study, we developed a biotin-streptavidin-amplified ELISA (BA-ELISA) using a produced monoclonal antibody for detecting pirlimycin residues in beef muscle, milk, and honey. The IC50 value of the BA-ELISA was 1.6 ng/mL for pirlimycin in buffer, and the sensitivity was improved 3 times compared to traditional ELISAs. The optimized BA-ELISA can be used to quantitate trace amounts of pirlimycin residues in beef muscle, milk, and honey. This method had limits of detection (LODs) of 4.45 μg/kg in beef muscle, 1.65 μg/L in milk, and 2.75 μg/kg in honey. The average recovery of the BA-ELISA ranged from 78 to 97%, and the coefficient of variation ranged from 5.3 to 13.5%. The developed BA-ELISA method was validated using LC-MS/MS, and the BA-ELISA can be used for routine screening analysis of pirlimycin residues.
authors.affiliation: School of Medicine, Health Science Center, Shenzhen University , Shenzhen 518060, China.
authors.forename: Wenxiao
authors.initials: W
authors.lastname: Jiang
authors.affiliation: Food and Feed Safety Research Unit, Southern Plains Agricultural Research Center, Agricultural Research Service, U.S. Department of Agriculture , 2881 F&B Road, College Station, Texas 77845, United States.
authors.forename: Ross C
authors.initials: RC
authors.lastname: Beier
authors.affiliation: Key Laboratory of Food Safety Risk Assessment, Ministry of Health, China National Center for Food Safety Risk Assessment , Beijing 100021, China.
authors.forename: Pengjie
authors.initials: P
authors.lastname: Luo
authors.affiliation: School of Medicine, Health Science Center, Shenzhen University , Shenzhen 518060, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhai
authors.affiliation: Key Laboratory of Food Safety Risk Assessment, Ministry of Health, China National Center for Food Safety Risk Assessment , Beijing 100021, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Wu
authors.affiliation: School of Medicine, Health Science Center, Shenzhen University , Shenzhen 518060, China.
authors.forename: Guimiao
authors.initials: G
authors.lastname: Lin
authors.affiliation: School of Medicine, Health Science Center, Shenzhen University , Shenzhen 518060, China.
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Wang
authors.affiliation: Institute of Optoelectronics, Department of Optoelectronic Information Science and Engineering, Shenzhen University , Shenzhen 518060, China.
authors.forename: Gaixia
authors.initials: G
authors.lastname: Xu
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D001710:Biotin / Q000737:chemistry; D002417:Cattle; D002981:Clindamycin / Q000031:analogs & derivatives* / Q000032:analysis; D004350:Drug Residues / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D006722:Honey / Q000032:analysis*; D008460:Meat / Q000032:analysis*; D008892:Milk / Q000737:chemistry*; D019809:Streptavidin / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D002981:Clindamycin; D001710:Biotin; D019809:Streptavidin; C034093:pirlimycin
keywords: beef muscle; food safety; honey; immunoassay; milk; pirlimycin