title: Methods to Determine the Lagrangian Shear Experienced by Platelets during Thrombus Growth.
abstract: Platelets can become activated in response to changes in flow-induced shear; however, the underlying molecular mechanisms are not clearly understood. Here we present new techniques for experimentally measuring the flow-induced shear rate experienced by platelets prior to adhering to a thrombus. We examined the dynamics of blood flow around experimentally grown thrombus geometries using a novel combination of experimental (ex vivo) and numerical (in silico) methodologies. Using a microcapillary system, platelet aggregate formation was analysed at elevated shear rates in the presence of coagulation inhibitors, where thrombus formation is predominantly platelet-dependent. These approaches permit the resolution and quantification of thrombus parameters at the scale of individual platelets (2 μm) in order to quantify real time thrombus development. Using our new techniques we can correlate the shear rate experienced by platelets with the extent of platelet adhesion and aggregation. The techniques presented offer the unique capacity to determine the flow properties for a temporally evolving thrombus field in real time.
authors.affiliation: Department of Mechanical and Aerospace Engineering, Monash University, Melbourne, Victoria, Australia.|Division of Biological Engineering, Faculty of Engineering, Monash University, Melbourne, Victoria, Australia.
authors.forename: Isaac P
authors.initials: IP
authors.lastname: Pinar
authors.affiliation: Australian Centre for Blood Diseases, Monash University, Melbourne, Victoria, Australia.
authors.forename: Jane F
authors.initials: JF
authors.lastname: Arthur
authors.affiliation: Australian Centre for Blood Diseases, Monash University, Melbourne, Victoria, Australia.
authors.forename: Robert K
authors.initials: RK
authors.lastname: Andrews
authors.affiliation: Australian Centre for Blood Diseases, Monash University, Melbourne, Victoria, Australia.
authors.forename: Elizabeth E
authors.initials: EE
authors.lastname: Gardiner
authors.affiliation: Department of Mechanical and Aerospace Engineering, Monash University, Melbourne, Victoria, Australia.
authors.forename: Kris
authors.initials: K
authors.lastname: Ryan
authors.affiliation: Department of Mechanical and Aerospace Engineering, Monash University, Melbourne, Victoria, Australia.|Division of Biological Engineering, Faculty of Engineering, Monash University, Melbourne, Victoria, Australia.
authors.forename: Josie
authors.initials: J
authors.lastname: Carberry
mesh_terms: D000925:Anticoagulants / Q000494:pharmacology; D001783:Blood Flow Velocity; D001792:Blood Platelets / Q000166:cytology* / Q000187:drug effects; D002478:Cells, Cultured; D006629:Hirudins / Q000494:pharmacology; D006801:Humans; D021621:Imaging, Three-Dimensional; D007700:Kinetics; D018613:Microscopy, Confocal; D015233:Models, Statistical; D010973:Platelet Adhesiveness / Q000187:drug effects; D010974:Platelet Aggregation / Q000187:drug effects; D013314:Stress, Mechanical; D013927:Thrombosis / Q000097:blood*
chemical_list: D000925:Anticoagulants; D006629:Hirudins