title: miR-362-3p targets nemo-like kinase and functions as a tumor suppressor in renal cancer cells.
abstract: MicroRNAs (miRNAs) have been demonstrated to exhibit abnormal expression patterns in various types of human cancer. The aim of the present study was to identify a novel tumor suppressor microRNA (miR) and investigate its physiological function and mechanism in renal cell carcinoma (RCC). The expression levels of miRNA (miR)‑362‑3p expres were measured in 47 pairs of RCC and adjacent normal tissue samples, using reverse transcription-quantitative polymerase chain reaction analysis. In addition, miR‑362‑3p was transfected into renal cancer cells to investigate its role in the regulation of cell proliferation, migration, invasion, apoptosis and cell cycle. Identification of the target gene of miR‑362‑3p was performed using luciferase reporter assays and western blot analyses. The results demonstrated that the expression levels of miR‑362‑3p were downregulated in the RCC tissue samples, compared with the adjacent normal tissue samples. The upregulation of miR‑362‑3p using a synthesized mimic suppressed the proliferation, migration and invasion of the renal cancer cells, and induced cell apoptosis and G1 phase arrest. Further experiments demonstrated that the overexpression of miR‑362‑3p resulted in decrease expression levels of nemo-like kinase. These results suggested that miR-362-3p functions as a tumor suppressor in RCC, and may serve as a potential molecular target in the treatment of RCC.
authors.affiliation: National‑Regional Key Technology Engineering Laboratory for Clinical Application of Cancer Genomics, Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, Guangdong 518000, P.R. China.
authors.forename: Xiaowen
authors.initials: X
authors.lastname: Zou
authors.affiliation: National‑Regional Key Technology Engineering Laboratory for Clinical Application of Cancer Genomics, Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, Guangdong 518000, P.R. China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Zhong
authors.affiliation: Department of Pediatric Urinary Surgery, Shenzhen Children's Hospital, Shenzhen, Guangdong 518000, P.R. China.
authors.forename: Jiaqiang
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Clinical Medicine, Shantou University Medical College, Shantou, Guangdong 515063, P.R. China.
authors.forename: Zhengming
authors.initials: Z
authors.lastname: Su
authors.affiliation: National‑Regional Key Technology Engineering Laboratory for Clinical Application of Cancer Genomics, Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, Guangdong 518000, P.R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.affiliation: National‑Regional Key Technology Engineering Laboratory for Clinical Application of Cancer Genomics, Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, Guangdong 518000, P.R. China.
authors.forename: Wanxin
authors.initials: W
authors.lastname: Deng
authors.affiliation: Department of Clinical Medicine, Shantou University Medical College, Shantou, Guangdong 515063, P.R. China.
authors.forename: Yuchi
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Clinical Medicine, Shantou University Medical College, Shantou, Guangdong 515063, P.R. China.
authors.forename: Siheng
authors.initials: S
authors.lastname: Lu
authors.affiliation: Department of Urology, Zhujiang Hospital of Southern Medical University, Guangzhou, Guangdong 510282, P.R. China.
authors.forename: Youcheng
authors.initials: Y
authors.lastname: Lin
authors.affiliation: National‑Regional Key Technology Engineering Laboratory for Clinical Application of Cancer Genomics, Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, Guangdong 518000, P.R. China.
authors.forename: Liya
authors.initials: L
authors.lastname: Luo
authors.affiliation: National‑Regional Key Technology Engineering Laboratory for Clinical Application of Cancer Genomics, Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, Guangdong 518000, P.R. China.
authors.forename: Zesong
authors.initials: Z
authors.lastname: Li
authors.affiliation: National‑Regional Key Technology Engineering Laboratory for Clinical Application of Cancer Genomics, Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, Guangdong 518000, P.R. China.
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Cai
authors.affiliation: National‑Regional Key Technology Engineering Laboratory for Clinical Application of Cancer Genomics, Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, Guangdong 518000, P.R. China.
authors.forename: Aifa
authors.initials: A
authors.lastname: Tang
mesh_terms: D017209:Apoptosis / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D016147:Genes, Tumor Suppressor; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000096:biosynthesis* / Q000235:genetics; D007680:Kidney Neoplasms / Q000235:genetics* / Q000473:pathology; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D009361:Neoplasm Invasiveness / Q000235:genetics / Q000473:pathology; D017346:Protein Serine-Threonine Kinases / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; C581582:MIRN362 microRNA, human; D035683:MicroRNAs; C470551:NLK protein, human; D017346:Protein Serine-Threonine Kinases