title: [Effects of Cordycepin on Proliferation, Apoptosis and NF-κB Signaling Pathway in A549 Cells].
abstract: OBJECTIVE
  To investigate the effects of Cordycepin on proliferation and apoptosis of A549 cells, and its possible mechanism of action.
  
  
  METHODS
  Human lung cancer cell line A549 cells were treated with different concentrations of Cordycepin for 24 h. MTS assay was used to detect cell proliferation. Hoechst 33258 staining and Annexin V/PI flow cytometry were used to determine the apoptosis of A549 cells. The protein expression of nuclear factor-κB p65 (NF-κB p65), BAX, BCL-2 and cleaved Caspase-3 was determined by Western blot.
  
  
  RESULTS
  In a dose dependent way, Cordycepin inhibited the proliferation and promoted the apoptosis of A549 cells, increased the expression of BAX and cleaved Caspase-3, while decreased the expression of BCL-2. The Western blot results showed, Cordycepin dose-dependently inhibited the entry of NF-κB p65 to nuclear in A59 cells.
  
  
  CONCLUSIONS
  Cordycepin can inhibit the proliferation and induct the apoptosis of A549 cells, the mechanism of action is achieved by inhibiting the NF-κB pathway.
authors.forename: Chao
authors.initials: C
authors.lastname: Zhang
authors.forename: Qin
authors.initials: Q
authors.lastname: Zhong
authors.forename: Xi-feng
authors.initials: XF
authors.lastname: Zhang
authors.forename: Dai-xi
authors.initials: DX
authors.lastname: Hu
authors.forename: Xue-mei
authors.initials: XM
authors.lastname: He
authors.forename: Quan-lun
authors.initials: QL
authors.lastname: Li
authors.forename: Tao
authors.initials: T
authors.lastname: Feng
mesh_terms: D017209:Apoptosis; D053148:Caspase 3 / Q000378:metabolism; D045744:Cell Line, Tumor / Q000187:drug effects; D049109:Cell Proliferation; D003839:Deoxyadenosines / Q000494:pharmacology*; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects*
chemical_list: D003839:Deoxyadenosines; D016328:NF-kappa B; C505407:CASP3 protein, human; D053148:Caspase 3; C058120:cordycepin