title: In vitro maturation affects chromosome segregation, spindle morphology and acetylation of lysine 16 on histone H4 in horse oocytes.
abstract: Implantation failure and genetic developmental disabilities in mammals are caused by errors in chromosome segregation originating mainly in the oocyte during meiosis I. Some conditions, like maternal ageing or in vitro maturation (IVM), increase the incidence of oocyte aneuploidy. Here oocytes from adult mares were used to investigate oocyte maturation in a monovulatory species. Experiments were conducted to compare: (1) the incidence of aneuploidy, (2) the morphology of the spindle, (3) the acetylation of lysine 16 on histone H4 (H4K16) and (4) the relative amount of histone acetyltransferase 1 (HAT1), K(lysine) acetyltransferase 8 (KAT8, also known as MYST1), histone deacetylase 1 (HDAC1) and NAD-dependent protein deacetylase sirtuin 1 (SIRT1) mRNA in metaphase II stage oocytes that were in vitro matured or collected from peri-ovulatory follicles. The frequency of aneuploidy and anomalies in spindle morphology was increased following IVM, along with a decrease in H4K16 acetylation that was in agreement with our previous observations. However, differences in the amount of the transcripts investigated were not detected. These results suggest that the degradation of transcripts encoding for histone deacetylases and acetyltransferases is not involved in the changes of H4K16 acetylation observed following IVM, while translational or post-translational mechanisms might have a role. Our study also suggests that epigenetic instabilities introduced by IVM may affect the oocyte and embryo genetic stability.
authors.affiliation: Reproductive and Developmental Biology Laboratory, Department of Health, Animal Science and Food Safety, University of Milan, via Celoria, 10, Milan, 20133, Italy.
authors.forename: Federica
authors.initials: F
authors.lastname: Franciosi
authors.affiliation: INRA, UMR85 Physiologie de la Reproduction et des Comportements, Nouzilly, F-37380, France.
authors.forename: Ghylene
authors.initials: G
authors.lastname: Goudet
authors.affiliation: Reproductive and Developmental Biology Laboratory, Department of Health, Animal Science and Food Safety, University of Milan, via Celoria, 10, Milan, 20133, Italy.
authors.forename: Irene
authors.initials: I
authors.lastname: Tessaro
authors.affiliation: INRA, UMR85 Physiologie de la Reproduction et des Comportements, Nouzilly, F-37380, France.
authors.forename: Pascal
authors.initials: P
authors.lastname: Papillier
authors.affiliation: INRA, UMR85 Physiologie de la Reproduction et des Comportements, Nouzilly, F-37380, France.
authors.forename: Rozenn
authors.initials: R
authors.lastname: Dalbies-Tran
authors.affiliation: INRA, UEPAO, Nouzilly, F-37380, France.
authors.forename: Fabrice
authors.initials: F
authors.lastname: Reigner
authors.affiliation: Université de Liège, Clinique des Animaux de Compagnie et des Équidés, Place du 20 Août 7, Liège, 4000, Belgium.
authors.forename: Stefan
authors.initials: S
authors.lastname: Deleuze
authors.affiliation: INRA, UMR85 Physiologie de la Reproduction et des Comportements, Nouzilly, F-37380, France.
authors.forename: Cecile
authors.initials: C
authors.lastname: Douet
authors.affiliation: University of Agricultural Sciences and Veterinary Medicine, Calea M?n?tur 3-5, Cluj-Napoca 400372, Romania.
authors.forename: Ileana
authors.initials: I
authors.lastname: Miclea
authors.affiliation: Reproductive and Developmental Biology Laboratory, Department of Health, Animal Science and Food Safety, University of Milan, via Celoria, 10, Milan, 20133, Italy.
authors.forename: Valentina
authors.initials: V
authors.lastname: Lodde
authors.affiliation: Reproductive and Developmental Biology Laboratory, Department of Health, Animal Science and Food Safety, University of Milan, via Celoria, 10, Milan, 20133, Italy.
authors.forename: Alberto M
authors.initials: AM
authors.lastname: Luciano
mesh_terms: D000107:Acetylation; D000818:Animals; D020090:Chromosome Segregation / Q000502:physiology*; D005260:Female; D006657:Histones / Q000378:metabolism*; D006736:Horses; D059471:In Vitro Oocyte Maturation Techniques; D008540:Meiosis / Q000502:physiology; D009865:Oocytes / Q000378:metabolism*; D009866:Oogenesis / Q000502:physiology; D008941:Spindle Apparatus / Q000502:physiology*
chemical_list: D006657:Histones