title: Toward a Universal Method for Preparing Molecularly Imprinted Polymer Nanoparticles with Antibody-like Affinity for Proteins.
abstract: We describe a potentially universal, simple and cheap method to prepare water-compatible molecularly imprinted polymer nanoparticles (MIP-NPs) as synthetic antibodies against proteins. The strategy is based on a solid phase synthesis approach where glass beads (GBs) are functionalized with a metal chelate, acting as a general affinity ligand to attract surface-bound histidines present on proteins. This configuration enables an oriented immobilization of the proteins, upon which thermoresponsive MIP-NPs are synthesized. The GBs play the role of both a reactor and a separation column since, after synthesis, the MIP-NPs are released from the support by a simple temperature change, resulting in protein-free polymers. The resulting MIP-NPs are endowed with improved binding site homogeneity, since the binding sites have the same orientation. Moreover, they are stable (no aggregation) in a buffer solution for prolonged storage time and exhibit apparent dissociation constants in the nanomolar range, with little or no cross-reactivity toward other proteins.
authors.affiliation: Sorbonne Universités, Université de Technologie de Compiègne , CNRS Enzyme and Cell Engineering Laboratory, Rue Roger Couttolenc, CS 60319, 60203 Compiègne Cedex, France.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Xu
authors.affiliation: Sorbonne Universités, Université de Technologie de Compiègne , CNRS Enzyme and Cell Engineering Laboratory, Rue Roger Couttolenc, CS 60319, 60203 Compiègne Cedex, France.
authors.forename: Serena
authors.initials: S
authors.lastname: Ambrosini
authors.affiliation: Hitit University , Faculty of Engineering, Department of Chemical Engineering, 19030 Çorum, Turkey.
authors.forename: Emel
authors.initials: E
authors.lastname: Tamahkar
authors.affiliation: Sorbonne Universités, Université de Technologie de Compiègne , CNRS Enzyme and Cell Engineering Laboratory, Rue Roger Couttolenc, CS 60319, 60203 Compiègne Cedex, France.
authors.forename: Claire
authors.initials: C
authors.lastname: Rossi
authors.affiliation: Sorbonne Universités, Université de Technologie de Compiègne , CNRS Enzyme and Cell Engineering Laboratory, Rue Roger Couttolenc, CS 60319, 60203 Compiègne Cedex, France.
authors.forename: Karsten
authors.initials: K
authors.lastname: Haupt
authors.affiliation: Sorbonne Universités, Université de Technologie de Compiègne , CNRS Enzyme and Cell Engineering Laboratory, Rue Roger Couttolenc, CS 60319, 60203 Compiègne Cedex, France.
authors.forename: Bernadette
authors.initials: B
authors.lastname: Tse Sum Bui
mesh_terms: D000906:Antibodies / Q000737:chemistry* / Q000276:immunology; D001665:Binding Sites; D054802:Molecular Imprinting / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism*; D060327:Solid-Phase Synthesis Techniques
chemical_list: D000906:Antibodies; D011108:Polymers; D011506:Proteins