title: Bianalyte multicommutated flow analysis system for microproteinuria diagnostics.
abstract: In this work a bianalyte multicommutated flow analysis (MCFA) system for determination of microproteinuria is presented. The developed MCFA system is based on two dedicated optoelectronic flow-through detectors which allow estimation of urinary protein creatinine ratio. For total protein determination, turbidimetric Exton's method was used, whereas creatinine was determined by the photometric Jaffe reaction. The developed analytical system is fully-mechanized, easy to operate, economic in reagent consumption and characterized by satisfactory analytical parameters. It allows protein determination in the range 36-300 mg L(-1) with 33 mg L(-1) detection limit and simultaneous determination of creatinine in the range 0.045-2.50 mmol L(-1) with 0.025 mmol L(-1) detection limit. The measurement procedure for the presented MCFA system offers performing 30 peaks per hour for both analytes. To prove the analytical usefulness of the system, real human urine samples have been analyzed. The correlation and agreement between results offered by the developed system and clinical analyzers are fully acceptable.
authors.affiliation: University of Warsaw, Department of Chemistry, Pasteura 1, 02-093 Warsaw, Poland; University of Warsaw, MISMaP College, Al. Żwirki i Wigury 93, 02-089 Warsaw, Poland. Electronic address: kamil.strzelak@chem.uw.edu.pl.
authors.forename: Kamil
authors.initials: K
authors.lastname: Strzelak
authors.affiliation: University of Warsaw, Department of Chemistry, Pasteura 1, 02-093 Warsaw, Poland.
authors.forename: Jagoda
authors.initials: J
authors.lastname: Misztal
authors.affiliation: University of Warsaw, Department of Chemistry, Pasteura 1, 02-093 Warsaw, Poland.
authors.forename: Łukasz
authors.initials: Ł
authors.lastname: Tymecki
authors.affiliation: University of Warsaw, Department of Chemistry, Pasteura 1, 02-093 Warsaw, Poland.
authors.forename: Robert
authors.initials: R
authors.lastname: Koncki
mesh_terms: D003404:Creatinine / Q000652:urine*; D055664:Electrochemical Techniques / Q000379:methods*; D017022:Flow Injection Analysis / Q000379:methods; D006801:Humans; D011507:Proteinuria / Q000175:diagnosis* / Q000652:urine*
chemical_list: D003404:Creatinine
keywords: Microproteinuria; Multicommutation; Paired emitter detector diode; Protein creatinine ratio