title: Microscopy as a diagnostic tool in pulmonary tuberculosis.
abstract: Tuberculosis continues to cast a huge impact on humanity with its high incidence and mortality, especially in developing countries. For tuberculosis case detection, microscopy continues to be indispensible, given its low cost, rapidity, simplicity of procedure and high specificity. Modifications have attempted to improve the sensitivity of microscopy which include: concentration methods such as centrifugation, N-acetyl cysteine-sodium hydroxide, bleach, ammonium sulfate or chitin. Furthermore, classical Ziehl-Neelsen (ZN) staining has been subjected to varying carbol fuchsin concentrations or replaced by Kinyoun staining, fluorescent microscopy or immune-fluorescence. Currently, light emitting diode fluorescence is recognizably the most plausible method as an alternative to ZN staining.
authors.affiliation: National Reference Laboratory & WHO Center of Excellence (Tuberculosis), Department of Microbiology, National Institute of Tuberculosis & Respiratory Diseases (NITRD), India. Electronic address: drritugo@gmail.com.
authors.forename: Ritu
authors.initials: R
authors.lastname: Singhal
authors.affiliation: National Reference Laboratory & WHO Center of Excellence (Tuberculosis), Department of Microbiology, National Institute of Tuberculosis & Respiratory Diseases (NITRD), India. Electronic address: tbmicro@gmail.com.
authors.forename: Vithal Prasad
authors.initials: VP
authors.lastname: Myneedu
mesh_terms: D006801:Humans; D008856:Microscopy, Fluorescence / Q000191:economics / Q000379:methods; D012680:Sensitivity and Specificity; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis*
keywords: Fluorescence; Microscopy; Mycobacteria; Tuberculosis; Ziehl–Neelsen