title: O-linked β-N-acetylglucosamine modification of proteins is activated in post-ischemic brains of young but not aged mice: Implications for impaired functional recovery from ischemic stress.
abstract: To evaluate the effect of age on the response of brains to an ischemic challenge, we subjected young and aged mice to transient forebrain ischemia, and analyzed the heat shock response and unfolded protein response, ubiquitin conjugation and SUMO conjugation, and O-linked β-N-acetylglucosamine modification of proteins (O-GlcNAcylation). The most prominent age-related difference was an inability of aged mice to activate O-GlcNAcylation. Considering many reports on the protective role of O-GlcNAcylation in various stress conditions including myocardial ischemia, this pathway could be a promising target for therapeutic intervention to improve functional recovery of aged patients following brain ischemia.
authors.affiliation: Laboratory of Molecular Neurobiology, Multidisciplinary Neuroprotection Laboratories, Department of Anesthesiology, Duke University Medical Center, Durham, North Carolina, USA.
authors.forename: Shuai
authors.initials: S
authors.lastname: Liu
authors.affiliation: Laboratory of Molecular Neurobiology, Multidisciplinary Neuroprotection Laboratories, Department of Anesthesiology, Duke University Medical Center, Durham, North Carolina, USA.
authors.forename: Huaxin
authors.initials: H
authors.lastname: Sheng
authors.affiliation: Laboratory of Molecular Neurobiology, Multidisciplinary Neuroprotection Laboratories, Department of Anesthesiology, Duke University Medical Center, Durham, North Carolina, USA Department of Critical Care Medicine, Renmin Hospital of Wuhan University, Wuhan, Hubei, China.
authors.forename: Zhui
authors.initials: Z
authors.lastname: Yu
authors.affiliation: Laboratory of Molecular Neurobiology, Multidisciplinary Neuroprotection Laboratories, Department of Anesthesiology, Duke University Medical Center, Durham, North Carolina, USA wulf.paschen@duke.edu wei.yang@duke.edu.
authors.forename: Wulf
authors.initials: W
authors.lastname: Paschen
authors.affiliation: Laboratory of Molecular Neurobiology, Multidisciplinary Neuroprotection Laboratories, Department of Anesthesiology, Duke University Medical Center, Durham, North Carolina, USA.
authors.forename: Wei
authors.initials: W
authors.lastname: Yang
mesh_terms: D000117:Acetylglucosamine / Q000378:metabolism*; D000375:Aging / Q000473:pathology*; D000818:Animals; D001921:Brain / Q000473:pathology*; D002545:Brain Ischemia / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology / Q000523:psychology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D017202:Myocardial Ischemia / Q000235:genetics / Q000503:physiopathology; D011499:Protein Processing, Post-Translational / Q000235:genetics*; D011597:Psychomotor Performance; D020127:Recovery of Function; D025842:SUMO-1 Protein / Q000378:metabolism; D013312:Stress, Physiological / Q000235:genetics; D020521:Stroke / Q000473:pathology* / Q000523:psychology*; D054875:Ubiquitination; D056811:Unfolded Protein Response / Q000235:genetics
chemical_list: D025842:SUMO-1 Protein; D000117:Acetylglucosamine
keywords: Aging; O-GlcNAc modification; SUMO conjugation; brain ischemia; ubiquitin conjugation; unfolded protein response