title: Effects of different antibacterial agents on enamel in a biofilm caries model.
abstract: Using a mature biofilm model, the aim of this study was to evaluate the effectiveness of different antibacterial agents in comparison with silver diamine fluoride (SDF). Forty-eight saliva-coated enamel slabs were inoculated with Streptococcus mutans monospecies biofilm. The biofilms were then exposed to 10% sucrose in tryptone yeast-extract culture medium, 8 times per day for 7 days. After the biofilm growth period, the enamel slabs were treated with one of the following substances: 1) distilled water; 2) SDF; 3) acidulated phosphate fluoride (APF); 4) ammonium hexafluorosilicate (AHF); 5) ammonium hexafluorosilicate + cetylpyridinium chloride (AHF+CPC); or 6) 0.2% chlorhexidine (CHX). After these treatment procedures, the samples were incubated at 37ºC for 2 days, and the numbers of viable microorganisms in the biofilms were counted. The number of viable bacteria was significantly reduced by all of the antibacterial agents (P < 0.05). However, SDF showed the highest antibacterial activity (P < 0.05), and the effectiveness of the other agents was lower (P < 0.05). SDF has a highly effective antibacterial action against cariogenic Streptococcus mutans biofilm; none of the other fluoride agents used in this study, or 0.2 CHX agent, showed an antibacterial effect comparable to that of SDF.
authors.affiliation: Department of Pediatric Dentistry, Faculty of Dentistry, Izmir Katip Celebi University.
authors.forename: Selcuk
authors.initials: S
authors.lastname: Savas
authors.forename: Ebru
authors.initials: E
authors.lastname: Kucukyılmaz
authors.forename: Esra U
authors.initials: EU
authors.lastname: Celik
authors.forename: Mustafa
authors.initials: M
authors.lastname: Ates
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D018441:Biofilms; D003731:Dental Caries / Q000382:microbiology*; D003743:Dental Enamel / Q000187:drug effects*; D006801:Humans; D008954:Models, Biological; D013295:Streptococcus mutans / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification*
chemical_list: D000900:Anti-Bacterial Agents