title: Differential regulation of senescence and in vitro differentiation by 17β-estradiol between mesenchymal stem cells derived from male and female mini-pigs.
abstract: The characterization and potential of mesenchymal stem cells (MSCs) are gender dependent and estrogen influences these properties. This study demonstrated that supplementation with 17β-estradiol (E2) increases the proliferation of bone marrow-MSCs derived from male and female mini-pigs (Mp- and Fp-BMSCs) in a concentration-dependent manner, with 10(-12) M E2 suggested as the optimal dose of E2 that led to the greatest improvement in BMSCs proliferation. Supplementation of 10(-12) M E2 resulted in down-regulation of β-galactosidase activity and pro-apoptotic activity in both BMSCs, while anti-apoptotic activity was up-regulated in only Fp-BMSCs. Further, E2 increased the osteogenic ability of Fp-BMSCs. Based on these findings, optimal utilization of E2 can improve cellular senescence and apoptosis, as well as in vitro osteogenesis of BMSCs, and could therefore be useful in stem cell therapy, particularly in bone regeneration for adult females.
authors.affiliation: College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Korea.|PWG Genetics Pte. Ltd., 15 Tech Park Crescent, Singapore 638117, Singapore.
authors.forename: Won-Jae
authors.initials: WJ
authors.lastname: Lee
authors.affiliation: College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Korea.
authors.forename: Seung-Chan
authors.initials: SC
authors.lastname: Lee
authors.affiliation: Advanced Therapy Products Research Division, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety, Cheongju 28159, Korea.
authors.forename: Jeong-Hyun
authors.initials: JH
authors.lastname: Lee
authors.affiliation: College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Korea.|Research Institute of Life Sciences, Gyeongsang National University, Jinju 52828, Korea.
authors.forename: Gyu-Jin
authors.initials: GJ
authors.lastname: Rho
authors.affiliation: College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Korea.|Research Institute of Life Sciences, Gyeongsang National University, Jinju 52828, Korea.
authors.forename: Sung-Lim
authors.initials: SL
authors.lastname: Lee
mesh_terms: D000818:Animals; D017209:Apoptosis; D002454:Cell Differentiation; D049109:Cell Proliferation; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000662:veterinary*; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D012737:Sex Factors; D013552:Swine; D013556:Swine, Miniature / Q000502:physiology*; D013997:Time Factors
chemical_list: D004958:Estradiol
keywords: 17 beta-estradiol; gender differences; mesenchymal stem cells; mini-pig; senescence