title: Involvement of the crustacean hyperglycemic hormone (CHH) in the physiological compensation of the freshwater crayfish Cherax quadricarinatus to low temperature and high salinity stress.
abstract: This study was aimed at determining the role of the crustacean hyperglycemic hormone (CHH) in the physiological compensation to both saline and thermal stress, in the freshwater crayfish Cherax quadricarinatus. By determining the expression of the CHH gene in the eyestalk of juvenile crayfish, we found that maximal induction of CHH was induced at high salinity (10 g/L) and low temperature (20 °C). In order to investigate the role of CHH in the physiological compensation to such stressful conditions, recombinant CHH was supplied to stressed animals. CHH-injected crayfish showed increased hemolymphatic levels of glucose, in accordance with a significant utilization of glycogen reserves from the hepatopancreas. Furthermore, CHH administration allowed stressed animals to regulate hemolymphatic sodium and potassium at more constant levels than controls. Taken together, these results suggest a relevant role of CHH in increasing the energy available intended for processes involved in the physiological compensation of C. quadricarinatus to both saline and thermal stress.
authors.affiliation: Department of Biodiversity and Experimental Biology, FCEN, University of Buenos Aires, Buenos Aires, Argentina.
authors.forename: Natalia C
authors.initials: NC
authors.lastname: Prymaczok
authors.affiliation: Department of Biodiversity and Experimental Biology, FCEN, University of Buenos Aires, Buenos Aires, Argentina.
authors.forename: Valeria M
authors.initials: VM
authors.lastname: Pasqualino
authors.affiliation: Department of Biodiversity and Experimental Biology, FCEN, University of Buenos Aires, Buenos Aires, Argentina.|Institute of Biodiversity, Experimental and Applied Biology (IBBEA), CONICET-UBA, Ciudad Universitaria, Pab. II, C1428EGA, Buenos Aires, Argentina.
authors.forename: Verónica E
authors.initials: VE
authors.lastname: Viau
authors.affiliation: Department of Biodiversity and Experimental Biology, FCEN, University of Buenos Aires, Buenos Aires, Argentina. enrique@bg.fcen.uba.ar.|Institute of Biodiversity, Experimental and Applied Biology (IBBEA), CONICET-UBA, Ciudad Universitaria, Pab. II, C1428EGA, Buenos Aires, Argentina. enrique@bg.fcen.uba.ar.
authors.forename: Enrique M
authors.initials: EM
authors.lastname: Rodríguez
authors.affiliation: Department of Biodiversity and Experimental Biology, FCEN, University of Buenos Aires, Buenos Aires, Argentina. medesani@bg.fcen.uba.ar.|Institute of Biodiversity, Experimental and Applied Biology (IBBEA), CONICET-UBA, Ciudad Universitaria, Pab. II, C1428EGA, Buenos Aires, Argentina. medesani@bg.fcen.uba.ar.
authors.forename: Daniel A
authors.initials: DA
authors.lastname: Medesani
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D060829:Arthropod Proteins / Q000008:administration & dosage / Q000235:genetics / Q000378:metabolism*; D003400:Astacoidea / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D003080:Cold Temperature; D017753:Ecosystem; D004734:Energy Metabolism / Q000187:drug effects; D005618:Fresh Water / Q000737:chemistry*; D005947:Glucose / Q000378:metabolism; D006003:Glycogen / Q000378:metabolism; D006458:Hemolymph / Q000378:metabolism; D043143:Hepatopancreas / Q000378:metabolism; D007447:Invertebrate Hormones / Q000008:administration & dosage / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000008:administration & dosage / Q000235:genetics / Q000378:metabolism*; D011188:Potassium / Q000378:metabolism; D011994:Recombinant Proteins / Q000008:administration & dosage; D054712:Salinity; D055049:Salt Tolerance; D015398:Signal Transduction; D012964:Sodium / Q000378:metabolism; D013312:Stress, Physiological
chemical_list: D060829:Arthropod Proteins; D007447:Invertebrate Hormones; D009419:Nerve Tissue Proteins; D011994:Recombinant Proteins; C015947:hyperglycemic hormone, crustacean; D006003:Glycogen; D012964:Sodium; D005947:Glucose; D011188:Potassium
keywords: CHH; Cherax quadricarinatus; Salinity; Stress; Temperature