title: Synthesis of the copper chelator TGTA and evaluation of its ability to protect biomolecules from copper induced degradation during copper catalyzed azide-alkyne bioconjugation reactions.
abstract: One of the most successful bioconjugation strategies to date is the copper(I)-catalyzed cycloaddition reaction (CuAAC), however, the typically applied reaction conditions have been found to degrade sensitive biomolecules. Herein, we present a water soluble copper chelator which can be utilized to protect biomolecules from copper induced degradation.
authors.affiliation: Department of Chemistry, University of Helsinki, PO Box 55, A. I. Virtasen aukio 1, FI-00014 Helsinki, Finland. filip.ekholm@helsinki.fi.
authors.forename: F S
authors.initials: FS
authors.lastname: Ekholm
authors.forename: H
authors.initials: H
authors.lastname: Pynnönen
authors.forename: A
authors.initials: A
authors.lastname: Vilkman
authors.forename: J
authors.initials: J
authors.lastname: Koponen
authors.forename: J
authors.initials: J
authors.lastname: Helin
authors.forename: T
authors.initials: T
authors.lastname: Satomaa
mesh_terms: D000480:Alkynes / Q000737:chemistry*; D001386:Azides / Q000737:chemistry*; D002384:Catalysis; D002614:Chelating Agents / Q000138:chemical synthesis* / Q000737:chemistry*; D003300:Copper / Q000737:chemistry*; D003500:Cyclization; D005697:Galactosides / Q000138:chemical synthesis / Q000737:chemistry*; D015394:Molecular Structure; D014230:Triazoles / Q000138:chemical synthesis / Q000737:chemistry*
chemical_list: D000480:Alkynes; D001386:Azides; D002614:Chelating Agents; D005697:Galactosides; D014230:Triazoles; C000611048:tris(6-galactosyltriazomethyl)amine; D003300:Copper