title: Heat shock protein 70-1A is a novel angiogenic regulator.
abstract: Heat shock protein 70-1A (HSP70-1A) is a stress-inducible protein that provides an essential intracellular molecular chaperone function; however, the mechanism of HSP70-1A in angiogenesis has not been clarified. Herein, HSP70-1A gene silencing implicated this protein in angiogenesis. Additionally, recombinant human HSP70-1A (rhHSP70-1A) was able to stimulate human umbilical vein endothelial cell (HUVEC) migration and tube formation in vitro and microvessel formation in vivo similarly to recombinant human vascular endothelial growth factor (rhVEGF). Furthermore, rhHSP70-1A was tightly bound to the surface of HUVECs and participated in extracellular signal-related kinase (ERK)-dependent angiogenesis. Together, these results implicate HSP70-1A as a novel angiogenic regulator.
authors.affiliation: Scripps Korea Antibody Institute, Hyoja-2-dong, Chuncheon-si, Gangwon-do, 200-701, South Korea.
authors.forename: Taek-Keun
authors.initials: TK
authors.lastname: Kim
authors.affiliation: Scripps Korea Antibody Institute, Hyoja-2-dong, Chuncheon-si, Gangwon-do, 200-701, South Korea.
authors.forename: Hee Jun
authors.initials: HJ
authors.lastname: Na
authors.affiliation: Scripps Korea Antibody Institute, Hyoja-2-dong, Chuncheon-si, Gangwon-do, 200-701, South Korea.
authors.forename: Woo Ran
authors.initials: WR
authors.lastname: Lee
authors.affiliation: Scripps Korea Antibody Institute, Hyoja-2-dong, Chuncheon-si, Gangwon-do, 200-701, South Korea.
authors.forename: Mee Hyun
authors.initials: MH
authors.lastname: Jeoung
authors.affiliation: Scripps Korea Antibody Institute, Hyoja-2-dong, Chuncheon-si, Gangwon-do, 200-701, South Korea. Electronic address: lees@skai.or.kr.
authors.forename: Sukmook
authors.initials: S
authors.lastname: Lee
mesh_terms: D001808:Blood Vessels / Q000201:enzymology* / Q000254:growth & development*; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D006801:Humans; D018919:Neovascularization, Physiologic / Q000502:physiology*
chemical_list: D018840:HSP70 Heat-Shock Proteins
keywords: Angiogenesis; ERK; HSP70-1A