title: ZDHHC16 modulates FGF/ERK dependent proliferation of neural stem/progenitor cells in the zebrafish telencephalon.
abstract: In vertebrates, neural stem/progenitor cells (NSPCs) maintenance is critical for nervous system development and homeostasis. However, the molecular mechanisms underlying the maintenance of NSPCs have not been fully elucidated. Here, we demonstrated that zebrafish ZDHHC16, a DHHC encoding protein, which was related to protein palmitoylation after translation, was expressed in the developing forebrain, and especially in the telencephalon. Loss- and gain-of-function studies showed that ZDHHC16 played a crucial role in the regualtion of NSPCs proliferation during zebrafish telencephalic development, via a mechanism dependent on its palmitoyltransferase activity. Further analyses showed that the inhibition of ZDHHC16 led to inactivation of the FGF/ERK signaling pathway during telencephalic NSPCs proliferation and maintenance. Taken together, our results suggest that ZDHHC16 activity is essential for early NSPCs proliferation where it acts to activate the FGF/ERK network, allowing for the initiation of proliferation -regulated gene expression programs. © 2016 Wiley Periodicals, Inc. Develop Neurobiol 76: 1014-1028, 2016.
authors.affiliation: Key Laboratory of the Ministry of Education for Experimental Teratology; Shandong Provincial Key Laboratory of Mental Disorders, Department of Histology and Embryology, Shandong University School of Medicine, No. 44, Wenhua Xi Road, Jinan, Shandong, 250012, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Shi
authors.affiliation: Center of Medical Physics and Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Anhui, 230031, People's Republic of China.
authors.forename: Xueran
authors.initials: X
authors.lastname: Chen
authors.affiliation: Key Laboratory of the Ministry of Education for Experimental Teratology; Shandong Provincial Key Laboratory of Mental Disorders, Department of Histology and Embryology, Shandong University School of Medicine, No. 44, Wenhua Xi Road, Jinan, Shandong, 250012, PR China.
authors.forename: Fen
authors.initials: F
authors.lastname: Wang
authors.affiliation: Reproductive Medical Center of Shandong University, Shandong University School of Medicine, Shandong, 250012, People's Republic of China.
authors.forename: Ming
authors.initials: M
authors.lastname: Gao
authors.affiliation: Infertility Center, Qilu Hospital, Shandong University School of Medicine, Shandong, 250012, People's Republic of China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Key Laboratory of the Ministry of Education for Experimental Teratology; Shandong Provincial Key Laboratory of Mental Disorders, Department of Histology and Embryology, Shandong University School of Medicine, No. 44, Wenhua Xi Road, Jinan, Shandong, 250012, PR China.
authors.forename: Zhaoxia
authors.initials: Z
authors.lastname: Du
authors.affiliation: Key Laboratory of the Ministry of Education for Experimental Teratology; Shandong Provincial Key Laboratory of Mental Disorders, Department of Histology and Embryology, Shandong University School of Medicine, No. 44, Wenhua Xi Road, Jinan, Shandong, 250012, PR China.
authors.forename: Chen
authors.initials: C
authors.lastname: Wang
authors.affiliation: Key Laboratory of the Ministry of Education for Experimental Teratology; Shandong Provincial Key Laboratory of Mental Disorders, Department of Histology and Embryology, Shandong University School of Medicine, No. 44, Wenhua Xi Road, Jinan, Shandong, 250012, PR China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Yao
authors.affiliation: Key Laboratory of the Ministry of Education for Experimental Teratology; Shandong Provincial Key Laboratory of Mental Disorders, Department of Histology and Embryology, Shandong University School of Medicine, No. 44, Wenhua Xi Road, Jinan, Shandong, 250012, PR China.
authors.forename: Kun
authors.initials: K
authors.lastname: He
authors.affiliation: Key Laboratory of the Ministry of Education for Experimental Teratology; Shandong Provincial Key Laboratory of Mental Disorders, Department of Histology and Embryology, Shandong University School of Medicine, No. 44, Wenhua Xi Road, Jinan, Shandong, 250012, PR China.
authors.forename: Aijun
authors.initials: A
authors.lastname: Hao
mesh_terms: D000217:Acyltransferases / Q000235:genetics / Q000502:physiology*; D000818:Animals; D049109:Cell Proliferation / Q000235:genetics / Q000502:physiology*; D004625:Embryo, Nonmammalian; D005346:Fibroblast Growth Factors / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental / Q000235:genetics / Q000502:physiology*; D020935:MAP Kinase Signaling System / Q000502:physiology*; D051379:Mice; D058953:Neural Stem Cells / Q000502:physiology*; D013687:Telencephalon / Q000502:physiology*; D015027:Zebrafish; D029961:Zebrafish Proteins / Q000235:genetics / Q000502:physiology*
chemical_list: D029961:Zebrafish Proteins; D005346:Fibroblast Growth Factors; D000217:Acyltransferases; C000609612:Zdhhc15b protein, zebrafish
keywords: FGF/ERK; ZDHHC16; neural stem/progenitor cell; palmitoylation; telencephalon