title: Phytofabrication of bioinduced silver nanoparticles for biomedical applications.
abstract: Synthesis of nanomaterials holds infinite possibilities as nanotechnology is revolutionizing the field of medicine by its myriad applications. Green synthesis of nanoparticles has become the need of the hour because of its eco-friendly, nontoxic, and economic nature. In this study, leaf extract of Rosa damascena was used as a bioreductant to reduce silver nitrate, leading to synthesis of silver nanoparticles (AgNPs) in a single step, without the use of any additional reducing or capping agents. The synthesized nanoparticles were characterized by the use of UV-visible spectroscopy, fourier transform infrared spectroscopy, dynamic light scattering, transmission electron microscopy, and field emission scanning electron microscopy. Time-dependent synthesis of AgNPs was studied spectrophotometrically. Synthesized AgNPs were found to possess flower-like spherical structure where individual nanoparticles were of 16 nm in diameter, whereas the agglomerated AgNPs were in the range of 60-80 nm. These biologically synthesized AgNPs exhibited significant antibacterial activity against Gram-negative bacterial species but not against Gram-positive ones (Escherichia coli and Bacillus cereus). Anti-inflammatory and analgesic activities were studied on a Wistar rat model to gauge the impact of AgNPs for a probable role in these applications. AgNPs tested positive for both these activities, although the potency was less as compared to the standard drugs.
authors.affiliation: School of Biotechnology, IFTM University, Lodhipur Rajput, Moradabad, Uttar Pradesh, India.
authors.forename: Nabeel
authors.initials: N
authors.lastname: Ahmad
authors.affiliation: School of Biotechnology, IFTM University, Lodhipur Rajput, Moradabad, Uttar Pradesh, India.
authors.forename: Sharad
authors.initials: S
authors.lastname: Bhatnagar
authors.affiliation: School of Pharmaceutical Sciences, IFTM University, Lodhipur Rajput, Moradabad, Uttar Pradesh, India.
authors.forename: Syed Salman
authors.initials: SS
authors.lastname: Ali
authors.affiliation: Institute of Bio-Science and Technology, Shri Ramswaroop Memorial University, Barabanki, Uttar Pradesh, India.
authors.forename: Rajiv
authors.initials: R
authors.lastname: Dutta
mesh_terms: D000700:Analgesics / Q000494:pharmacology / Q000627:therapeutic use; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000893:Anti-Inflammatory Agents / Q000494:pharmacology / Q000627:therapeutic use; D020811:Biomedical Technology / Q000379:methods*; D004195:Disease Models, Animal; D000067493:Dynamic Light Scattering; D004926:Escherichia coli / Q000187:drug effects; D007249:Inflammation / Q000188:drug therapy / Q000473:pathology; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D008826:Microbial Sensitivity Tests; D019800:Phenol / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D020011:Protective Agents / Q000494:pharmacology / Q000627:therapeutic use; D017208:Rats, Wistar; D027826:Rosa / Q000737:chemistry*; D012834:Silver / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature
chemical_list: D000700:Analgesics; D000900:Anti-Bacterial Agents; D000893:Anti-Inflammatory Agents; D010936:Plant Extracts; D020011:Protective Agents; D019800:Phenol; D012834:Silver
keywords: analgesic; animal model study; anti-inflammatory; antibacterial; green synthesis; silver nanoparticles