title: Single-Particle Tracking of Human Lipoproteins.
abstract: Lipoproteins, such as high-density lipoprotein (HDL), low-density lipoprotein (LDL), and very-low density lipoprotein (VLDL), play a critical role in heart disease. Lipoproteins vary in size and shape as well as in their apolipoprotein content. Here, we developed a new experimental framework to study freely diffusing lipoproteins from human blood, allowing analysis of even the smallest HDL with a radius of 5 nm. In an easily constructed confinement chamber, individual HDL, LDL, and VLDL particles labeled with three distinct fluorophores were simultaneously tracked by wide-field fluorescence microscopy and their sizes were determined by their motion. This technique enables studies of individual lipoproteins in solution and allows characterization of the heterogeneous properties of lipoproteins which affect their biological function but are difficult to discern in bulk studies.
authors.affiliation: Laboratory of Molecular Biophysics and ‡Lipoprotein Metabolism Section, National Heart, Lung, and Blood Institute, National Institutes of Health , Bethesda, Maryland 20892, United States.
authors.forename: Michel
authors.initials: M
authors.lastname: de Messieres
authors.affiliation: Laboratory of Molecular Biophysics and ‡Lipoprotein Metabolism Section, National Heart, Lung, and Blood Institute, National Institutes of Health , Bethesda, Maryland 20892, United States.
authors.forename: Abby
authors.initials: A
authors.lastname: Ng
authors.affiliation: Laboratory of Molecular Biophysics and ‡Lipoprotein Metabolism Section, National Heart, Lung, and Blood Institute, National Institutes of Health , Bethesda, Maryland 20892, United States.
authors.forename: Cornelio J
authors.initials: CJ
authors.lastname: Duarte
authors.affiliation: Laboratory of Molecular Biophysics and ‡Lipoprotein Metabolism Section, National Heart, Lung, and Blood Institute, National Institutes of Health , Bethesda, Maryland 20892, United States.
authors.forename: Alan T
authors.initials: AT
authors.lastname: Remaley
authors.affiliation: Laboratory of Molecular Biophysics and ‡Lipoprotein Metabolism Section, National Heart, Lung, and Blood Institute, National Institutes of Health , Bethesda, Maryland 20892, United States.
authors.forename: Jennifer C
authors.initials: JC
authors.lastname: Lee
mesh_terms: D004058:Diffusion; D006801:Humans; D008074:Lipoproteins / Q000097:blood*; D008856:Microscopy, Fluorescence; D009038:Motion; D010316:Particle Size; D013499:Surface Properties
chemical_list: D008074:Lipoproteins