title: Molecular mapping and marker development for the Triticum dicoccoides-derived stripe rust resistance gene YrSM139-1B in bread wheat cv. Shaanmai 139.
abstract: CONCLUSIONS
  YrSM139-1B maybe a new gene for effective resistance to stripe rust and useful flanking markers for marker-assisted selection were developed.
  Stripe rust, caused by Puccinia striiformis f. sp. tritici, is an important foliar disease of wheat. Two dominant stripe rust resistant genes YrSM139-1B and YrSM139-2D were pyramided in bread wheat cultivar Shaanmai 139; one from wild emmer and the other from Thinopyrum intermedium. Three near-isogenic F7:8 line pairs (contrasting RILs), N122-1013R/S, N122-185R/S, and N122-1812R/S, independently derived from different F2 plants and differing at the YrSM139-1B locus were generated from the cross Shaanmai 139 × Hu 901-19 through marker-assisted selection. A large F2:3 population from cross N122-1013R × N122-1013S tested for stripe rust response and subjected to analysis with markers in the 1BS10-0.5 bin region using SSR expressed sequence tags (EST) and site-specific sequence markers developed from the 90 K Illumina iSelect SNP array. Five EST-STS markers and four allele-specific PCR markers were mapped to the YrSM139-1B region. The 30.5 cM genetic map for YrSM139-1B consisted of nine markers, two of which were closer to YrSM139-1B than Xgwm273, which was used in producing the contrasting RIL pairs. Race response data and allelism tests showed that YrSM139-1B is different from Yr10, Yr15, and Yr24/26/CH42.
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy (Northwest A&F University), Yangling, 712100, Shaanxi, China. zhangh1129@nwafu.edu.cn.|College of Plant Protection, Northwest A&F University, Yangling, 712100, Shaanxi, China. zhangh1129@nwafu.edu.cn.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy (Northwest A&F University), Yangling, 712100, Shaanxi, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy (Northwest A&F University), Yangling, 712100, Shaanxi, China.
authors.forename: Changyou
authors.initials: C
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy (Northwest A&F University), Yangling, 712100, Shaanxi, China.
authors.forename: Yajuan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Plant Protection, Northwest A&F University, Yangling, 712100, Shaanxi, China.
authors.forename: Xinli
authors.initials: X
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy (Northwest A&F University), Yangling, 712100, Shaanxi, China.
authors.forename: Shikai
authors.initials: S
authors.lastname: Lv
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy (Northwest A&F University), Yangling, 712100, Shaanxi, China.
authors.forename: Xinlun
authors.initials: X
authors.lastname: Liu
authors.affiliation: College of Plant Protection, Northwest A&F University, Yangling, 712100, Shaanxi, China. kangzs@nwsuaf.edu.cn.
authors.forename: Zhensheng
authors.initials: Z
authors.lastname: Kang
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy (Northwest A&F University), Yangling, 712100, Shaanxi, China. jiwanquan2003@126.com.
authors.forename: Wanquan
authors.initials: W
authors.lastname: Ji
mesh_terms: D000483:Alleles; D001487:Basidiomycota; D002874:Chromosome Mapping; D018744:DNA, Plant / Q000235:genetics; D060467:Disease Resistance / Q000235:genetics*; D020224:Expressed Sequence Tags; D017343:Genes, Plant; D005819:Genetic Markers; D018895:Microsatellite Repeats; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology; D016324:Sequence Tagged Sites; D014908:Triticum / Q000235:genetics* / Q000382:microbiology
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers