title: Comparative proteomic analysis of fibrotic liver of rats fed high fat diet contained lard versus corn oil.
abstract: The influences of dietary fatty acids on the progress of chronic liver diseases have attracted lots of attentions, but the mechanisms of the effects of lipids rich in saturated fatty acids or PUFAs on hepatic fibrogenesis remain unclear.
  
  
  
  Female Fischer 344 rats were fed normal chow or chow plus 20% (w/w) of corn oil or lard, respectively, and injected CCl4twice a week for 4 weeks to induce liver fibrosis. Masson's staining was adopted to illustrate the fibrosis level. The mRNA expression level of α-SMA and the DNA methylation level of its promoter region were analyzed. A 2-DE gel based proteomic approach was constructed to investigate the differential expression level of hepatic proteome between three diet groups.
  
  
  
  Histological evaluations and α-SMA expression analysis illustrated the high corn oil intake has no effects on hepatic fibrogenesis, but lard intake aggravated liver fibrosis, partly attributed to DNA demethylation of α-SMA promoter region. 2-DE Gel based proteomic study demonstrated excessive lard consumption elevated the expression of fibrosis related alpha-1-antitrypsin precursor, and endoplasmic reticulum stress related proteins such as heat shock cognate 71 kDa, eukaryotic translation initiation factor 4A1 and protein disulfide isomerase associated 3. Moreover, unlike corn oil rich in PUFAs, lard had no effects to elevate the expression of glutathione S-transferases, but decreased the expression of iron store related proteins heme binding protein 1 and ferritin.
  
  
  
  Lard intake aggravates CCl4induced liver fibrosis via enhancing the expression of fibrogenesis and ER stress related proteins, and disturbing the hepatic transmethylation reaction.
authors.affiliation: School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, Hubei, China; Food and Nutrition Division, School of Biological Sciences, The University of Hong Kong, Hong Kong, China.
authors.forename: Hualin
authors.initials: H
authors.lastname: Wang
authors.affiliation: Food and Nutrition Division, School of Biological Sciences, The University of Hong Kong, Hong Kong, China.
authors.forename: Wat-Hung
authors.initials: WH
authors.lastname: Sit
authors.affiliation: Department of Anatomy, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China.
authors.forename: George Lim
authors.initials: GL
authors.lastname: Tipoe
authors.affiliation: School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, Hubei, China.
authors.forename: Zhiguo
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Food and Nutrition Division, School of Biological Sciences, The University of Hong Kong, Hong Kong, China. Electronic address: jmfwan@hku.hk.
authors.forename: Jennifer Man-Fan
authors.initials: JM
authors.lastname: Wan
mesh_terms: D000199:Actins / Q000235:genetics / Q000378:metabolism; D000818:Animals; D002251:Carbon Tetrachloride; D003314:Corn Oil / Q000008:administration & dosage*; D019175:DNA Methylation; D059305:Diet, High-Fat / Q000009:adverse effects*; D004041:Dietary Fats / Q000008:administration & dosage* / Q000009:adverse effects; D004721:Endoplasmic Reticulum / Q000187:drug effects / Q000378:metabolism; D005227:Fatty Acids / Q000008:administration & dosage / Q000009:adverse effects; D005260:Female; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008103:Liver Cirrhosis / Q000139:chemically induced / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D020543:Proteome; D051381:Rats; D011916:Rats, Inbred F344; D015203:Reproducibility of Results
chemical_list: D000199:Actins; D002251:Carbon Tetrachloride; D003314:Corn Oil; D004041:Dietary Fats; D005227:Fatty Acids; D005982:Glutathione Transferase; D020543:Proteome; C498862:Acta2 protein, rat; C029310:lard
keywords: Carbon tetrachloride; Corn oil; Lard; Liver fibrosis; Proteomics