title: [Association of SPO11 and GST gene polymorphisms with idiopathic male infertility in ethnic Han Chinese].
abstract: OBJECTIVE
  To explore the possible roles of polymorphisms of SPO11 and glutathionine S-transferase (GST) genes in idiopathic male infertility in a ethnic Han Chinese population from Henan.
  
  
  METHODS
  Multiplex PCR and DNA sequencing were performed to determine the SPO11 c.517C>T(rs28368082) and GST genes (GSTM1, GSTT1, GSTP1) polymorphisms in 216 idiopathic male infertility cases and 198 normal samples.
  
  
  RESULTS
  The frequencies of the SPO11 CC and CT genotypes were 87.5% (189/216) and 12.5% (27/216) in the patients, and 97.5% (193/198) and 2.5% (5/198) in the controls, respectively. The frequencies of SPO11 CC and CT genotypes, the A>G transition at nucleotide 313 in the exon 5 of the GSTP1 gene, and the frequencies of combined genotypes GSTM1 (-/-), GSTT1 (+/+), GSTP1 (AA) and SPO11 (CT) were significantly different between the two groups (P<0.05).
  
  
  CONCLUSIONS
  The rs28368082 polymorphism of the SPO11 gene, the A>G transition at nucleotide 313 in the exon 5 of the GSTP1 gene, and the combined genotypes of GSTM1 (-/-), GSTT1 (+/+), GSTP1 (AA) and SPO11 (CT) may be associated with idiopathic male infertility in ethnic Han Chinese.
authors.affiliation: Department of Urology, The First People's Hospital of Zhengzhou, Zhengzhou, Henan 450004, P.R. China. fengzhanqi448590@sina.com.
authors.forename: Zhanqi
authors.initials: Z
authors.lastname: Feng
authors.forename: Zhian
authors.initials: Z
authors.lastname: Jing
authors.forename: Hongyan
authors.initials: H
authors.lastname: Liu
authors.forename: Shixiu
authors.initials: S
authors.lastname: Liao
authors.forename: Liangjie
authors.initials: L
authors.lastname: Guo
authors.forename: Changqing
authors.initials: C
authors.lastname: Mao
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Liu
authors.forename: Hui
authors.initials: H
authors.lastname: Wu
authors.forename: Jiangtao
authors.initials: J
authors.lastname: Gao
mesh_terms: D000328:Adult; D000483:Alleles; D044466:Asian People / Q000235:genetics; D001483:Base Sequence; D002681:China; D004706:Endodeoxyribonucleases / Q000235:genetics*; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease / Q000208:ethnology / Q000235:genetics*; D005838:Genotype; D051549:Glutathione S-Transferase pi / Q000235:genetics*; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D007248:Infertility, Male / Q000201:enzymology / Q000208:ethnology / Q000235:genetics*; D015810:Linkage Disequilibrium; D008297:Male; D009154:Mutation; D016017:Odds Ratio; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D000099257:Meiotic Recombination Protein SPO11
chemical_list: D004706:Endodeoxyribonucleases; D051549:Glutathione S-Transferase pi; D005982:Glutathione Transferase; D000099257:Meiotic Recombination Protein SPO11; C413545:glutathione S-transferase T1