title: Detection of Japanese eel endothelial cells-infecting virus in Anguilla japonica elvers.
abstract: Japanese eel endothelial cells-infecting virus (JEECV) has spread in eel farms and caused serious economic loss. In this study, we examined the prevalence of JEECV infection in 100 wild Japanese eel (Anguilla japonica) elvers caught from Yamaguchi prefecture, Japan, using quantitative PCR and conventional PCR. Total genomic DNA was obtained from the cranial quarter of the body in 70 of 100 eels and from the gill in the remaining. Of 30 gill samples, 20 were analyzed after pooling with other samples, and the remaining 10 were analyzed separately. A single positive result for JEECV was detected following analysis of the 10 separately analyzed samples. This result constitutes the first report of JEECV infection in wild A. japonica elvers.
authors.affiliation: The United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagito, Gifu-shi, Gifu 501-1193, Japan.
authors.forename: Sachiko
authors.initials: S
authors.lastname: Okazaki
authors.forename: Shinya
authors.initials: S
authors.lastname: Yasumoto
authors.forename: Satoshi
authors.initials: S
authors.lastname: Koyama
authors.forename: Shinobu
authors.initials: S
authors.lastname: Tsuchiaka
authors.forename: Yuki
authors.initials: Y
authors.lastname: Naoi
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Omatsu
authors.forename: Shin-Ichi
authors.initials: S
authors.lastname: Ono
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Mizutani
mesh_terms: D000811:Anguilla / Q000821:virology*; D000818:Animals; D005393:Fish Diseases / Q000821:virology*; D005398:Fisheries; D007564:Japan; D027382:Polyomaviridae; D027601:Polyomavirus Infections / Q000662:veterinary*; D014412:Tumor Virus Infections / Q000662:veterinary*