title: Enzymatic in situ saccharification of chestnut shell with high ionic liquid-tolerant cellulases from Galactomyces sp. CCZU11-1 in a biocompatible ionic liquid-cellulase media.
abstract: In this study, it was the first time to report that the cellulases of Galactomyces sp. CCZU11-1 showed high activity and stability in the culture and reaction media containing IL [Mmim]DMP. Using untreated chestnut shell (CNS) as carbon source in the culture media containing IL [Mmim]DMP (5%, w/v), high activity of FPA (28.6U/mL), xylanase (186.2U/mL), and CMCase (107.3U/mL) were obtained, and 184.9mg/L of total protein was achieved. Furthermore, the changes in the structural features (crystallinity, morphology, and porosity) of the solid residue of CNS utilized with Galactomyces sp. CCZU11-1 were characterized with Fourier transform infrared spectroscopy, scanning electron microscopy, and X-ray diffraction. After was enzymatically hydrolyzed with the prepared crude enzymes in IL diluted to 20% (w/v), a high yield of reducing sugars, 62.1%, was obtained. Significantly, Galactomyces sp. CCZU11-1 showed high potential for the efficient transformation of lignocellulosic materials to glucose in a single-step process.
authors.affiliation: Platform of Bioethanol, Laboratory of Biochemical Engineering, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China; Bioproducts, Sciences and Engineering Laboratory, Department of Biological Systems Engineering, Washington State University, Richland, WA 99354, USA. Electronic address: heyucai2001@163.com.
authors.forename: Yu-Cai
authors.initials: YC
authors.lastname: He
authors.affiliation: Platform of Bioethanol, Laboratory of Biochemical Engineering, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Liu
authors.affiliation: Platform of Bioethanol, Laboratory of Biochemical Engineering, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Gong
authors.affiliation: Platform of Bioethanol, Laboratory of Biochemical Engineering, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China.
authors.forename: Jun-Hua
authors.initials: JH
authors.lastname: Di
authors.affiliation: Platform of Bioethanol, Laboratory of Biochemical Engineering, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Ding
authors.affiliation: Bioproducts, Sciences and Engineering Laboratory, Department of Biological Systems Engineering, Washington State University, Richland, WA 99354, USA.
authors.forename: Cui-Luan
authors.initials: CL
authors.lastname: Ma
authors.affiliation: Platform of Bioethanol, Laboratory of Biochemical Engineering, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China.
authors.forename: Dan-Ping
authors.initials: DP
authors.lastname: Zhang
authors.affiliation: Platform of Bioethanol, Laboratory of Biochemical Engineering, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China.
authors.forename: Zhi-Cheng
authors.initials: ZC
authors.lastname: Tao
authors.affiliation: Platform of Bioethanol, Laboratory of Biochemical Engineering, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Wang
authors.affiliation: Bioproducts, Sciences and Engineering Laboratory, Department of Biological Systems Engineering, Washington State University, Richland, WA 99354, USA.
authors.forename: Bin
authors.initials: B
authors.lastname: Yang
mesh_terms: D002480:Cellulase / Q000737:chemistry*; D044602:Cellulases / Q000737:chemistry*; D002482:Cellulose / Q000737:chemistry*; D003470:Culture Media; D031256:Eleocharis / Q000737:chemistry*; D057075:Enzyme Assays; D006868:Hydrolysis; D052578:Ionic Liquids / Q000737:chemistry; D004718:Saccharomycetales / Q000201:enzymology*; D014961:X-Ray Diffraction
chemical_list: D003470:Culture Media; D052578:Ionic Liquids; D002482:Cellulose; D044602:Cellulases; D002480:Cellulase; C019826:carboxymethylcellulase
keywords: Biocompatibility; Cellulase; Galactomyces sp. CCZU11-1; In situ saccharification; Ionic liquid