title: Influence of polymer molecular weight on the in vitro cytotoxicity of poly (N-isopropylacrylamide).
abstract: Poly (N-isopropylacrylamide) (PNIPAAm) is a thermosensitive polymer with various biomedical applications. We examined molecular weight (MW)-dependent cytotoxicity of PNIPAAm. Our results indicated that low-MW PNIPAAm (degree of polymerization (DP)=35) is inherently toxic to cells. Moderate-MW PNIPAAms with their DP between 100 and 200 are non-cytotoxic. When cells are seeded on top of a polymer-coated surface, PNIPAAm with a higher MW (DP=400) shows non/low cytotoxicity, while when monolayer cells are exposed to the polymer solution, cell viability drops drastically. This may be due to lack of nutrient and oxygen rather than intrinsic toxicity of the polymer.
authors.affiliation: School of Chemical Engineering, The University of Adelaide, Adelaide SA5005, Australia. Electronic address: amir.mellati@adelaide.edu.au.
authors.forename: Amir
authors.initials: A
authors.lastname: Mellati
authors.affiliation: School of Chemical Engineering, The University of Adelaide, Adelaide SA5005, Australia. Electronic address: meisam.valizadehkiamahalleh@adelaide.edu.au.
authors.forename: Meisam
authors.initials: M
authors.lastname: Valizadeh Kiamahalleh
authors.affiliation: School of Chemical Engineering, The University of Adelaide, Adelaide SA5005, Australia. Electronic address: s.dai@adelaide.edu.au.
authors.forename: Sheng
authors.initials: S
authors.lastname: Dai
authors.affiliation: School of Chemical Engineering, The University of Adelaide, Adelaide SA5005, Australia. Electronic address: jingxiu.bi@adelaide.edu.au.
authors.forename: Jingxiu
authors.initials: J
authors.lastname: Bi
authors.affiliation: School of Chemical Engineering, The University of Adelaide, Adelaide SA5005, Australia. Electronic address: bo.jin@adelaide.edu.au.
authors.forename: Bo
authors.initials: B
authors.lastname: Jin
authors.affiliation: School of Chemical Engineering, The University of Adelaide, Adelaide SA5005, Australia. Electronic address: hu.zhang@adelaide.edu.au.
authors.forename: Hu
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry* / Q000633:toxicity*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects*; D016503:Drug Delivery Systems; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D008970:Molecular Weight; D013696:Temperature
chemical_list: D000180:Acrylic Resins; C052970:poly-N-isopropylacrylamide
keywords: Cytotoxicity; Drug delivery; Poly (N-isopropylacrylamide); Polymer molecular weight; Thermosensitive hydrogel; Tissue engineering