title: [Study of blood concentration analysis for formate in acute methanol poisoning].
abstract: A 53-year-old woman ingested about 300 mL of 95% methanol. After immediate ethanol antagonist therapy and hemodialysis, she recovered completely. Few days later, the plasma concentration of methanol and formate was measured. A gas chromatography was used for the plasma methanol concentration measurement, and a colorimetric method was used for plasma formate concentration measurement (Formate Colorimetric Assay Kit; BioVision, California, USA). Patient's plasma methanol concentration before hemodialysis was 676.9 mg/dL and plasma formate concentration was 16.9 mg/dL. By removing blood methanol and formate using hemodialysis before formate accumulations in the body, the patient was discharged without any sequelae. We were able to obtain correlation between a gas chromatography and colorimetric method without gas chromatography-mass spectrometry, with good correlation coefficients. The sensitivity was sufficient for analyzing blood sample. Monitoring formate concentration is useful in determining the treatment and evaluating the prognosis of methanol poisoning. We suggest that this colorimetric method is useful in a facility with no access to a gas chromatography in order to measure a plasma formate concentration.
authors.forename: Go
authors.initials: G
authors.lastname: Morikawa
authors.forename: Katsuko
authors.initials: K
authors.lastname: Okazawa
authors.forename: Takahiro
authors.initials: T
authors.lastname: Shimizu
authors.forename: Sayoko
authors.initials: S
authors.lastname: Otagiri
authors.forename: Fumiko
authors.initials: F
authors.lastname: Fuwa
authors.forename: Saori
authors.initials: S
authors.lastname: Nakagawa
authors.forename: Susumu
authors.initials: S
authors.lastname: Yamato
mesh_terms: D000208:Acute Disease; D000437:Alcoholism / Q000175:diagnosis*; D002849:Chromatography, Gas; D005260:Female; D005561:Formates / Q000097:blood*; D006801:Humans; D000432:Methanol / Q000097:blood / Q000506:poisoning*; D008875:Middle Aged; D006435:Renal Dialysis
chemical_list: D005561:Formates; C030544:formic acid; D000432:Methanol