title: Interaction of C60 fullerene complexed to doxorubicin with model bilipid membranes and its uptake by HeLa cells.
abstract: With an aim to elucidate the effects of C60 fullerene complexed with antibiotic doxorubicin (Dox) on model bilipid membranes (BLM), the investigation of the electrical properties of BLM under the action of Dox and C60 fullerene, and of their complex, C60+Dox,was performed. The complex as well as its components exert a clearly detectable influence on BLM, which is concentration-dependent and also depends on phospholipid composition. The mechanism of this effect originates either from intermolecular interaction of the drug with fatty-acid residues of phospholipids, or from membranotropic effects of the drug-induced lipid peroxidation, or from the sum of these two effects. By fluorescence microscopy the entering of C60 + Dox complex into HeLa cells was directly shown.
authors.affiliation: Taras Shevchenko National University of Kyiv, Volodymyrska Str., 64, 01601 Kyiv, Ukraine. Electronic address: prylut@ukr.net.
authors.forename: Yu
authors.initials: Y
authors.lastname: Prylutskyy
authors.affiliation: Taras Shevchenko National University of Kyiv, Volodymyrska Str., 64, 01601 Kyiv, Ukraine.
authors.forename: A
authors.initials: A
authors.lastname: Bychko
authors.affiliation: Institute of Inorganic Chemistry and Center for Nanointegration Duisburg-Essen (CeNIDE), University of Duisburg-Essen, Universitaetsstr. 5-7, 45117 Essen, Germany.
authors.forename: V
authors.initials: V
authors.lastname: Sokolova
authors.affiliation: Taras Shevchenko National University of Kyiv, Volodymyrska Str., 64, 01601 Kyiv, Ukraine.
authors.forename: S
authors.initials: S
authors.lastname: Prylutska
authors.affiliation: Department of Physics, Sevastopol State University, Sevastopol 299053, Crimea; Belgorod State University, Pobedy Str., 85, 308015 Belgorod, Russia. Electronic address: max_evstigneev@mail.ru.
authors.forename: M
authors.initials: M
authors.lastname: Evstigneev
authors.affiliation: Taras Shevchenko National University of Kyiv, Volodymyrska Str., 64, 01601 Kyiv, Ukraine.
authors.forename: V
authors.initials: V
authors.lastname: Rybalchenko
authors.affiliation: Institute of Inorganic Chemistry and Center for Nanointegration Duisburg-Essen (CeNIDE), University of Duisburg-Essen, Universitaetsstr. 5-7, 45117 Essen, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Epple
authors.affiliation: Technical University of Ilmenau, Institute of Chemistry and Biotechnology, Weimarer Str. 25, 98693 Ilmenau, Germany.
authors.forename: P
authors.initials: P
authors.lastname: Scharff
mesh_terms: D004317:Doxorubicin / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D004337:Drug Carriers / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D037741:Fullerenes / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D008051:Lipid Bilayers / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects*
chemical_list: D004337:Drug Carriers; D037741:Fullerenes; D008051:Lipid Bilayers; D004317:Doxorubicin; C069837:fullerene C60
keywords: C(60) fullerene; Current–voltage characteristics; Doxorubicin; Fluorescence microscopy; HeLa cells; Model bilipid membranes