title: CD1a+ and CD83+ Langerhans cells are reduced in lower lip squamous cell carcinoma.
abstract: BACKGROUND
  Actinic cheilitis (AC) is a potentially malignant lesion diagnosed in the lip of patients chronically exposed to the sun that may give rise to a fully invasive lower lip squamous cell carcinoma (LLSCC). It is known that ultraviolet radiation causes dendritic cells (DCs) depletion in the epidermis, but the role of this cellular population in lip cancer progression remains uncertain. Therefore, this study investigated the distribution of DCs in normal, dysplastic and neoplastic tissues of the lower lip.
  
  
  METHODS
  Thirteen cases of lower lip mucocele, 42 of ACs and 21 of LLSCC were retrieved and original diagnoses confirmed by two oral pathologists, who further classified ACs as low- and high-risk lesions. Immunoreactions against CD1a and CD83 identified immature and mature DCs, respectively.
  
  
  RESULTS
  Immature CD1a+ Langerhans cells (LCs) were significantly decreased in LLSCC when compared to morphologically normal (P < 0.009) and dysplastic epitheliums (P < 0.003), whereas mature CD83+ LCs were significantly decreased in LLSCC when compared to normal epithelium (P = 0.038). There was no significant difference between low- and high-risk ACs regarding CD1a+ and CD83+ LCs (P > 0.05), but ACs demonstrated a lower concentration of CD1a+ LCs than normal epithelium (P < 0.009). There was no significant difference in the distribution of CD1a+ and CD83+ interstitial dendritic cells (IDCs) in the connective tissue among the studied groups (P > 0.05).
  
  
  CONCLUSIONS
  These results suggest that depletion of epithelial LCs, but not IDCs in the connective tissue, would represent an important step for lip cancer development.
authors.affiliation: Department of Oral Diagnosis, University of Campinas, Piracicaba, Brazil.
authors.forename: Juliane Oliveira
authors.initials: JO
authors.lastname: Gomes
authors.affiliation: Department of Dentistry, University of Pernambuco, Recife, Brazil.
authors.forename: Marianne
authors.initials: M
authors.lastname: de Vasconcelos Carvalho
authors.affiliation: Department of Oral Diagnosis, University of Campinas, Piracicaba, Brazil.
authors.forename: Felipe Paiva
authors.initials: FP
authors.lastname: Fonseca
authors.affiliation: Department of Pathology, Federal University of Santa Catarina, Florianópolis, Brazil.
authors.forename: Rogério Oliveira
authors.initials: RO
authors.lastname: Gondak
authors.affiliation: Department of Oral Diagnosis, University of Campinas, Piracicaba, Brazil.
authors.forename: Márcio Ajudarte
authors.initials: MA
authors.lastname: Lopes
authors.affiliation: Department of Oral Diagnosis, University of Campinas, Piracicaba, Brazil.|Department of Oral Pathology and Oral Biology, Faculty of Health Sciences, School of Dentistry, University of Pretoria, Pretoria, South Africa.
authors.forename: Pablo Agustin
authors.initials: PA
authors.lastname: Vargas
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000276:immunology*; D018949:Antigens, CD1 / Q000276:immunology*; D002294:Carcinoma, Squamous Cell / Q000150:complications / Q000000981:diagnostic imaging / Q000378:metabolism* / Q000473:pathology*; D002613:Cheilitis / Q000276:immunology / Q000473:pathology; D002648:Child; D003713:Dendritic Cells / Q000276:immunology / Q000473:pathology; D005260:Female; D006258:Head and Neck Neoplasms / Q000150:complications / Q000000981:diagnostic imaging / Q000378:metabolism* / Q000473:pathology*; D006801:Humans; D007136:Immunoglobulins / Q000276:immunology*; D007801:Langerhans Cells / Q000276:immunology* / Q000473:pathology*; D008048:Lip Neoplasms / Q000000981:diagnostic imaging / Q000276:immunology / Q000473:pathology*; D008297:Male; D008562:Membrane Glycoproteins / Q000276:immunology*; D008875:Middle Aged; D009078:Mucocele / Q000276:immunology / Q000473:pathology; D000077195:Squamous Cell Carcinoma of Head and Neck; D055815:Young Adult; D000097802:CD83 Antigen
chemical_list: D015703:Antigens, CD; D018949:Antigens, CD1; D007136:Immunoglobulins; D008562:Membrane Glycoproteins; D000097802:CD83 Antigen
keywords: CD1a; CD83; Langerhans cells; actinic cheilitis; lip squamous cell carcinoma