title: Anterior chamber interleukin 1β, interleukin 6 and prostaglandin E2 in patients undergoing femtosecond laser-assisted cataract surgery.
abstract: OBJECTIVE
  To evaluate the interleukin (IL) 1β, IL-6 and prostaglandin E2 (PGE2) concentrations in aqueous humour in patients undergoing femtosecond laser-assisted cataract surgery.
  
  
  METHODS
  In 27 eyes of 27 patients undergoing femtosecond laser-assisted cataract surgery (femto group), aqueous humour of 100 μL was collected after laser treatment. In 15 eyes of 15 subjects undergoing routine cataract surgery (control group), aqueous humour of 100 μL was also collected. The IL-1β, IL-6 and Prostaglandin E2 (Human Interleukin 1β, Interleukin 6 and Prostaglandin E2 ELISA Kits, Bio-Swamp Life Science) were used to determine the concentrations of IL-1β, IL-6 and PGE2 in the aqueous humour. All patients were treated with non-steroidal anti-inflammatory drugs prior to surgery.
  
  
  RESULTS
  For IL-1β, IL-6 and PGE2, respectively, the mean concentration values in aqueous humour were 25.6, 24.6 and 64.2 pg/mL in the femto group, and 17.1, 15.2 and 45.7 pg/mL in the control group (table 2). Concentrations of IL-1β, IL-6 and PGE2 were significantly higher in the femto group than those in the control group (all p<0.01). There were no significant correlations between concentrations of IL-1β, IL-6 or PGE2 and age, cataract densities, suction time or laser time (all p>0.05).
  
  
  CONCLUSIONS
  Inflammatory cytokines IL-1β, IL-6 and PGE2 significantly increased after femtosecond laser-assisted cataract surgery, which maybe the cause of intraoperative miosis seen in these patients.
authors.affiliation: Shanxi Eye Hospital, Taiyuan, Shanxi Province, P. R. China Department of Ophthalmology, Cullen Eye Institute, Baylor College of Medicine, Houston, Texas, USA.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.affiliation: Shanxi Eye Hospital, Taiyuan, Shanxi Province, P. R. China Clinical College of Ophthalmology, Tianjin Medical University, Tianjin, P. R. China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Ophthalmology, Cullen Eye Institute, Baylor College of Medicine, Houston, Texas, USA.
authors.forename: Douglas D
authors.initials: DD
authors.lastname: Koch
authors.affiliation: Shanxi Eye Hospital, Taiyuan, Shanxi Province, P. R. China.
authors.forename: Yading
authors.initials: Y
authors.lastname: Jia
authors.affiliation: Shanxi Eye Hospital, Taiyuan, Shanxi Province, P. R. China.
authors.forename: Weifang
authors.initials: W
authors.lastname: Cao
authors.affiliation: Shanxi Eye Hospital, Taiyuan, Shanxi Province, P. R. China.
authors.forename: Suhua
authors.initials: S
authors.lastname: Zhang
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000867:Anterior Chamber / Q000378:metabolism; D001082:Aqueous Humor / Q000378:metabolism*; D002386:Cataract / Q000378:metabolism*; D002387:Cataract Extraction / Q000379:methods*; D015232:Dinoprostone / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D053583:Interleukin-1beta / Q000378:metabolism*; D015850:Interleukin-6 / Q000378:metabolism*; D053685:Laser Therapy / Q000379:methods*; D008297:Male; D008875:Middle Aged
chemical_list: C508600:IL6 protein, human; D053583:Interleukin-1beta; D015850:Interleukin-6; D015232:Dinoprostone
keywords: Immunology; Inflammation; Lens and zonules; Treatment Lasers; Treatment Surgery