title: Quantitation of Flecainide, Mexiletine, Propafenone, and Amiodarone in Serum or Plasma Using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS).
abstract: Flecainide, mexiletine, propafenone, and amiodarone are antiarrhythmic drugs that are used primarily in the treatment of cardiac arrhythmias. The monitoring of the use of these drugs has applications in therapeutic drug monitoring and overdose situations. LC-MS/MS is used to analyze plasma/serum extracts with loxapine as the internal standard to ensure accurate quantitation and control for any potential matrix effects. Positive ion electrospray is used to introduce the analytes into the mass spectrometer. Selected reaction monitoring of two product ions for each analyte allows for the calculation of ion ratios which ensures correct identification of each analyte, while a matrix matched calibration curve is used for quantitation.
authors.affiliation: ARUP Institute for Clinical and Experimental Pathology, 500 Chipeta Way, 209-D02, Salt Lake City, UT, 84108, USA. matthew.slawson@aruplab.com.|ARUP Laboratories, 500 Chipeta Way, 209-D02, Salt Lake City, UT, 84108, USA. matthew.slawson@aruplab.com.
authors.forename: Matthew H
authors.initials: MH
authors.lastname: Slawson
authors.affiliation: Department of Pathology, University of Utah Health Sciences Center, School of Medicine, ARUP Institute for Clinical and Experimental Pathology, 500 Chipeta Way, 209-D02, Salt Lake City, UT, 84108, USA.
authors.forename: Kamisha L
authors.initials: KL
authors.lastname: Johnson-Davis
mesh_terms: D000638:Amiodarone / Q000097:blood*; D000889:Anti-Arrhythmia Agents / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D016903:Drug Monitoring / Q000379:methods*; D005424:Flecainide / Q000097:blood*; D006801:Humans; D008801:Mexiletine / Q000097:blood*; D011405:Propafenone / Q000097:blood*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000889:Anti-Arrhythmia Agents; D008801:Mexiletine; D011405:Propafenone; D005424:Flecainide; D000638:Amiodarone
keywords: Amiodarone; Flecainide; Mass spectrometry; Mexiletine; Plasma; Propafenone; Serum; UPLC