title: Blood gas analyses and other components involved in the acid-base metabolism of rats infected by Trypanosoma evansi.
abstract: The aim of this study was to investigate the effects of Trypanosoma evansi infections on arterial blood gases of experimentally infected rats. Two groups with eight animals each were used; group A (uninfected) and group B (infected). Infected animals were daily monitored through blood smears that showed high parasitemia with 30 trypanosomes per field (1000×) on average, 5 days post-infection (PI). Arterial blood was collected at 5 days PI for blood gas analysis using an automated method based on dry-chemistry. Hydrogen potential (pH), partial oxygen pressure (pO2), oxygen saturation (sO2), sodium (Na), ionic calcium (Ca ionic), chlorides (Cl), partial dioxide carbon pressure (pCO2), base excess (BE), base excess in the extracellular fluid (BEecf), bicarbonate (cHCO3), potassium (K), lactate, and blood total dioxide the carbon (tCO2) were evaluated. The levels of pH, pCO2, BE, BEecf, cHCO3, and tCO2 were significantly decreased (P < 0.05) in group B compared to group A. Additionally, the same group showed increases in Cl and lactate levels when compared to uninfected group. Therefore, it is possible to state that the infection caused by T. evansi led to alterations in the acid-base status, findings that are correlated to metabolic acidosis.
authors.affiliation: Centro Universitário Franciscano, Santa Maria, RS, Brazil ; Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil.
authors.forename: Matheus D
authors.initials: MD
authors.lastname: Baldissera
authors.affiliation: Centro Universitário Franciscano, Santa Maria, RS, Brazil.
authors.forename: Rodrigo A
authors.initials: RA
authors.lastname: Vaucher
authors.affiliation: Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil.
authors.forename: Camila B
authors.initials: CB
authors.lastname: Oliveira
authors.affiliation: Laboratory of Nanotechnology, Centro Universitário Franciscano, Santa Maria, RS, Brazil.
authors.forename: Virginia C
authors.initials: VC
authors.lastname: Rech
authors.affiliation: Centro Universitário Franciscano, Santa Maria, RS, Brazil.
authors.forename: Michele R
authors.initials: MR
authors.lastname: Sagrillo
authors.affiliation: Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil.
authors.forename: Daniel R
authors.initials: DR
authors.lastname: Stainki
authors.affiliation: Department of Small Animals, Universidade Federal de Santa Maria, Brazil.
authors.forename: Raqueli T
authors.initials: RT
authors.lastname: França
authors.affiliation: Laboratory of Veterinary Epidemiology, Faculty of Veterinary, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil.
authors.forename: Gustavo
authors.initials: G
authors.lastname: Machado
authors.affiliation: Department of Small Animals, Universidade Federal de Santa Maria, Brazil.
authors.forename: Sonia T A
authors.initials: ST
authors.lastname: Lopes
authors.affiliation: Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil.
authors.forename: Silvia G
authors.initials: SG
authors.lastname: Monteiro
authors.affiliation: Department of Animal Science, Universidade do Estado de Santa Catarina (UDESC), Chapecó, SC, Brazil.
authors.forename: Lenita M
authors.initials: LM
authors.lastname: Stefani
authors.affiliation: Department of Animal Science, Universidade do Estado de Santa Catarina (UDESC), Chapecó, SC, Brazil.
authors.forename: Aleksandro S
authors.initials: AS
authors.lastname: Da Silva
keywords: Arterial blood gases; Pathology; Protozoan; “Surra”