title: [MEDICAGO SATIVA L: IMPROVEMENT AND NEW APPROACHES OF ITS NUTRITIONAL AND FUNCTIONAL VALUE BY BACTERIAL CO-INOCULATION].
abstract: OBJECTIVE
  to study the effect of co-inoculation with Ensifer meliloti and Halomonas maura of the leguminous Medicago sativa L., on growth, nutritional and functional value, grown under salinity conditions.
  
  
  METHODS
  plants of M. sativa were grown in a solution with a mixture of salts (CaSO4, MgCl, NaCl and NaHCO 3) and were co-inoculated with its specific rhizobium and the halophilic moderated bacterium H. maura. Different physiologic parameters were determined, as well as, nitrogen and minerals content. Furthermore, an assay of in vitro digestibility was carried out.
  
  
  RESULTS
  salinity had a negative effect on the plants; however, co-inoculation increased yield, nitrogen content, total minerals, Ca and Mg. Moreover, physiologic parameters as water potential and leghemoglobin content in fresh nodules were higher compared to those of plants inoculated only with E. meliloti. Both, salinity and bacterial treatment with E. meliloti and H. maura increased the antioxidant capacity of the legume, in dialyzates and retentates collected after an in vitro digestibility assay.
  
  
  CONCLUSIONS
  co-inoculation of plants with E. meliloti and H. maura could improve the alfalfa yield under specific salinity conditions, increasing the nutritional and functional value of the plants. M. sativa could be considered in the formulations of nutritional supplements for the human diet.
authors.affiliation: Departamento de Fisiología, Instituto de Nutrición y Tecnología de los Alimentos, Facultad de Farmacia, Universidad de Granada, Granada.. rosariomz@ugr.es.
authors.forename: Rosario
authors.initials: R
authors.lastname: Martínez
authors.affiliation: Departamento de Fisiología, Instituto de Nutrición y Tecnología de los Alimentos, Facultad de Farmacia, Universidad de Granada, Granada.. enebot@ugr.es.
authors.forename: Elena
authors.initials: E
authors.lastname: Nebot
authors.affiliation: Departamento de Fisiología, Instituto de Nutrición y Tecnología de los Alimentos, Facultad de Farmacia, Universidad de Granada, Granada.. jmporres@ugr.es.
authors.forename: Jesús María
authors.initials: JM
authors.lastname: Porres
authors.affiliation: Departamento de Fisiología, Instituto de Nutrición y Tecnología de los Alimentos, Facultad de Farmacia, Universidad de Granada, Granada.. rosariomz@ugr.es.
authors.forename: Garyfallia
authors.initials: G
authors.lastname: Kapravelou
authors.affiliation: Departamento de Microbiología, Facultad de Farmacia, Universidad de Granada, Granada.. admoral@ugr.es.
authors.forename: Ana
authors.initials: A
authors.lastname: Del Moral
authors.affiliation: Departamento de Microbiología de Suelos y Sistemas Simbióticos, Estación Experimental del Zaidín (CSIC), Granada, España.. chouhra@hotmail.com.
authors.forename: Chouhra
authors.initials: C
authors.lastname: Talbi
authors.affiliation: Departamento de Microbiología de Suelos y Sistemas Simbióticos, Estación Experimental del Zaidín (CSIC), Granada, España.. eulogio.bedmar@eez.csic.es.
authors.forename: Eulogio Jose
authors.initials: EJ
authors.lastname: Bedmar
authors.affiliation: Departamento de Fisiología, Instituto de Nutrición y Tecnología de los Alimentos, Facultad de Farmacia, Universidad de Granada, Granada.. mlopezj@ugr.es.
authors.forename: María
authors.initials: M
authors.lastname: López-Jurado
mesh_terms: D000975:Antioxidants / Q000494:pharmacology; D019587:Dietary Supplements; D004063:Digestion; D055951:Functional Food / Q000032:analysis*; D020632:Halomonas; D007874:Leghemoglobin / Q000032:analysis; D000455:Medicago sativa / Q000737:chemistry*; D008903:Minerals / Q000032:analysis; D009753:Nutritive Value; D019936:Probiotics / Q000032:analysis*; D016962:Sinorhizobium meliloti
chemical_list: D000975:Antioxidants; D007874:Leghemoglobin; D008903:Minerals