title: The hematopoietic regulator, ELF-1, enhances the transcriptional response to Interferon-β of the OAS1 anti-viral gene.
abstract: Interferon (IFN) therapy is effective in treating cancers, haematological and virus induced diseases. The classical Jak/Stat pathway of IFN signal transduction leading to changes in transcriptional activity is well established but alone does not explain the whole spectrum of cellular responses to IFN. Gene promoters contain cis-acting sequences that allow precise and contextual binding of transcription factors, which control gene expression. Using the transcriptional response to IFN as a starting point we report a high frequency of tandem GGAA motifs in the proximal promoters of Interferon stimulated genes, suggesting a key regulatory action. Utilizing the well-characterized anti-viral gene, OAS1, as an example Interferon stimulated gene promoter containing such a duplicated GGAA motif, we have demonstrated a regulatory role of this promoter in response to IFN by mutation analysis. Furthermore, we identified ELF-1 as a direct binding factor at this motif. Additionally, recruitment of RB1 and SP1 factors to the promoter following IFN stimulation is shown. ELF-1 overexpression enhanced and knockdown of ELF-1 inhibited full activation of OAS1 by IFN stimulation. Collectively, ELF-1 binds an important duplicated GGAA cis-acting element at the OAS1 promoter and in cooperation with RB1 and SP1 recruitment contributes to regulation in response to IFN stimulation.
authors.affiliation: Research Center for RNA Science, RIST, Tokyo University of Science, Noda, Chiba, Japan.|Department of Gene Regulation, Faculty of Pharmaceutical Sciences, Tokyo University of Science, Noda, Chiba, Japan.
authors.forename: Steven
authors.initials: S
authors.lastname: Larsen
authors.affiliation: Department of Gene Regulation, Faculty of Pharmaceutical Sciences, Tokyo University of Science, Noda, Chiba, Japan.
authors.forename: Shota
authors.initials: S
authors.lastname: Kawamoto
authors.affiliation: Research Center for RNA Science, RIST, Tokyo University of Science, Noda, Chiba, Japan.|Department of Biochemistry, Faculty of Pharmaceutical Sciences, Tokyo University of Science, Noda, Chiba, Japan.
authors.forename: Sei-ichi
authors.initials: S
authors.lastname: Tanuma
authors.affiliation: Research Center for RNA Science, RIST, Tokyo University of Science, Noda, Chiba, Japan.|Department of Gene Regulation, Faculty of Pharmaceutical Sciences, Tokyo University of Science, Noda, Chiba, Japan.
authors.forename: Fumiaki
authors.initials: F
authors.lastname: Uchiumi
mesh_terms: D015088:2',5'-Oligoadenylate Synthetase / Q000235:genetics*; D001483:Base Sequence; D001665:Binding Sites; D002460:Cell Line; D060467:Disease Resistance / Q000235:genetics*; D036383:Ephrin-A2 / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D006367:HeLa Cells; D054884:Host-Pathogen Interactions; D006801:Humans; D050835:Interferon Regulatory Factors / Q000235:genetics; D016899:Interferon-beta / Q000378:metabolism*; D008969:Molecular Sequence Data; D059372:Nucleotide Motifs; D010766:Phosphorylation; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D016160:Retinoblastoma Protein / Q000378:metabolism; D016329:Sp1 Transcription Factor / Q000378:metabolism; D014158:Transcription, Genetic; D015533:Transcriptional Activation
chemical_list: D036383:Ephrin-A2; D050835:Interferon Regulatory Factors; D016160:Retinoblastoma Protein; D016329:Sp1 Transcription Factor; D016899:Interferon-beta; C475085:OAS1 protein, human; D015088:2',5'-Oligoadenylate Synthetase