title: Rck1 promotes pseudohyphal growth via the activation of Ubp3 phosphorylation in Saccharomyces cerevisiae.
abstract: Previously, we reported that Rck1 up-regulates Ras2 and pseudohyphal growth of Saccharomyces cerevisiae. Here, we further investigate the involvement of Rck1 in the activation of pseudohyphal growth. Rck1 activated phosphorylation of the deubiquitinase Ubp3 through a direct protein interaction between Rck1 and Ubp3. The N-terminal Bre5 binding region of Ubp3 physically interacted with Rck1, and Ubp3 and Rck1 co-precipitated. Overexpression of UBP3 using a high-copy plasmid resulted in the upregulation of Ras2, and deletion of UBP3 blocked the upregulation of Ras2 by RCK1 overexpression. Treatment with the proteasome inhibitor MG132 resulted in accumulation of Ras2, indicating that Rck1 is involved in Ras2 degradation in a proteasome-dependent manner. Furthermore, deletion of UBP3 blocked the upregulation of FLO11, a flocculin required for pseudohyphal and invasive growth induced by RCK1 overexpression in S. cerevisiae. Taken together, these results demonstrate that Rck1 promotes S. cerevisiae pseudohyphal growth via the activation of Ubp3 phosphorylation.
authors.affiliation: School of Life Sciences and Biotechnology, Korea University, Anam-dong, Sungbuk-gu, Seoul, Republic of Korea.
authors.forename: Chang-Min
authors.initials: CM
authors.lastname: Kang
authors.affiliation: School of Life Sciences and Biotechnology, Korea University, Anam-dong, Sungbuk-gu, Seoul, Republic of Korea.
authors.forename: Miwha
authors.initials: M
authors.lastname: Chang
authors.affiliation: School of Life Sciences and Biotechnology, Korea University, Anam-dong, Sungbuk-gu, Seoul, Republic of Korea.
authors.forename: Yong-Sung
authors.initials: YS
authors.lastname: Park
authors.affiliation: School of Life Sciences and Biotechnology, Korea University, Anam-dong, Sungbuk-gu, Seoul, Republic of Korea. Electronic address: cheolwony@korea.ac.kr.
authors.forename: Cheol-Won
authors.initials: CW
authors.lastname: Yun
mesh_terms: D049108:Cell Enlargement; D049109:Cell Proliferation / Q000502:physiology; D010450:Endopeptidases / Q000378:metabolism*; D004789:Enzyme Activation; D025301:Hyphae / Q000254:growth & development*; D037241:Mannose-Binding Lectins / Q000378:metabolism*; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D015854:Up-Regulation / Q000502:physiology
chemical_list: C087578:FLO1 protein, S cerevisiae; D037241:Mannose-Binding Lectins; D029701:Saccharomyces cerevisiae Proteins; D017346:Protein Serine-Threonine Kinases; C407463:RCK1 protein, S cerevisiae; D010450:Endopeptidases; C101111:UBP3 protein, S cerevisiae
keywords: Pseudohyphal growth; Ras2; Rck1; S. cerevisiae; Ubp3