title: Guanidine hydrochloride embedded polyurethanes as antimicrobial and absorptive wound dressing membranes with promising cytocompatibility.
abstract: Preparation and assessments of novel absorptive wound dressing materials with efficient antimicrobial activity as well as very good cytocompatibility were described in this work. An amine terminated poly(hexamethylene guanidine hydrochloride) was prepared and used as curing agent of different epoxy-terminated polyurethane prepolymers. The structures of prepared materials were elucidated by evaluation of their (1)H NMR and FTIR spectra. The recorded tensile strength of membranes confirmed the excellent dimensional stability of the film type dressings even at fully hydrated conditions. Therefore, these dressings could protect the wound bed from external forces during the healing period. The structurally optimized dressing membranes could preserve the desired moist environment over the wounded area, as a result of their balanced equilibrium, water absorption and water vapor transmission rate. Therefore, a very good condition for stimulation of self-healing of wound bed was attained. Also, owing to the presence of guanidine hydrochloride moieties embedded into the structure of dressings, efficient antimicrobial activity against Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans were detected. In vitro cytotoxicity assay of the prepared dressings revealed cytocompatibility of these materials against fibroblast cells. Therefore, they could support cell growth and proliferation at the wounded area.
authors.affiliation: Polyurethane Department, Iran Polymer and Petrochemical Institute, P.O. Box: 14965/115, Tehran, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Sahraro
authors.affiliation: Polyurethane Department, Iran Polymer and Petrochemical Institute, P.O. Box: 14965/115, Tehran, Iran. Electronic address: h.yeganeh@ippi.ac.ir.
authors.forename: Hamid
authors.initials: H
authors.lastname: Yeganeh
authors.affiliation: Polyurethane Department, Iran Polymer and Petrochemical Institute, P.O. Box: 14965/115, Tehran, Iran.
authors.forename: Marziyeh
authors.initials: M
authors.lastname: Sorayya
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry* / Q000494:pharmacology / Q000633:toxicity; D001419:Bacteria / Q000187:drug effects; D001458:Bandages; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology / Q000633:toxicity; D002176:Candida albicans / Q000187:drug effects; D002460:Cell Line; D019791:Guanidine / Q000737:chemistry* / Q000494:pharmacology / Q000633:toxicity; D008422:Materials Testing; D051379:Mice; D011140:Polyurethanes / Q000737:chemistry* / Q000494:pharmacology / Q000633:toxicity
chemical_list: D000890:Anti-Infective Agents; D001672:Biocompatible Materials; D011140:Polyurethanes; D019791:Guanidine
keywords: Antimicrobial; Poly(hexamethylene guanidine hydrochloride); Polyurethane; Wound dressing