title: Enumeration and Molecular Characterization of Tumor Cells in Lung Cancer Patients Using a Novel In Vivo Device for Capturing Circulating Tumor Cells.
abstract: The use of circulating tumor cells (CTC) as "liquid biopsy" is limited by the very low yield of CTCs available for subsequent analyses. Most in vitro approaches rely on small sample volumes (5-10 mL).
  
  
  
  Here, we used a novel approach, the GILUPI CellCollector, which enables an in vivo isolation of CTCs from peripheral blood. In total, 50 lung cancer patients were screened in two subsequent device applications before and after therapy (n = 185 applications).
  
  
  
  By in vivo isolation, 58% (108/185) of the patients were positive for ≥1 CTC (median, 5 CTCs; range, 1-56 cells) as compared with 27% (23/84; range, 1-300 cells) using the FDA-cleared CellSearch system. Furthermore, we could show that treatment response during therapy was associated with significant decreases in CTC counts (P = 0.001). By dPCR, mutations in the KRAS and EGFR genes relevant for treatment decisions could be detected in CTCs captured by in vivo isolation and confirmed in the primary tumors of the same patients.
  
  
  
  In vivo isolation of CTCs overcomes blood volume limitations of other approaches, which might help to implement CTC-based "liquid biopsies" into clinical decision making. Clin Cancer Res; 22(9); 2197-206. ©2015 AACR.
authors.affiliation: Department of Tumor Biology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
authors.forename: Tobias M
authors.initials: TM
authors.lastname: Gorges
authors.affiliation: Department of Pneumology, University Clinic Ulm, Ulm, Germany.
authors.forename: Nicole
authors.initials: N
authors.lastname: Penkalla
authors.affiliation: Department of Pneumology, University Clinic Ulm, Ulm, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Schalk
authors.affiliation: Department of Tumor Biology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
authors.forename: Simon A
authors.initials: SA
authors.lastname: Joosse
authors.affiliation: Department of Tumor Biology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
authors.forename: Sabine
authors.initials: S
authors.lastname: Riethdorf
authors.affiliation: GILUPI GmbH, Potsdam/Greifswald, Germany.
authors.forename: Johannes
authors.initials: J
authors.lastname: Tucholski
authors.affiliation: GILUPI GmbH, Potsdam/Greifswald, Germany.
authors.forename: Klaus
authors.initials: K
authors.lastname: Lücke
authors.affiliation: Department of Tumor Biology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
authors.forename: Harriet
authors.initials: H
authors.lastname: Wikman
authors.affiliation: Thermo Fisher Scientific, South San Francisco, California.
authors.forename: Stephen
authors.initials: S
authors.lastname: Jackson
authors.affiliation: Bayer Pharma AG, Berlin, Germany.
authors.forename: Nora
authors.initials: N
authors.lastname: Brychta
authors.affiliation: Bayer Pharma AG, Berlin, Germany.
authors.forename: Oliver
authors.initials: O
authors.lastname: von Ahsen
authors.affiliation: Department of Pneumology, University Clinic Ulm, Ulm, Germany. Clinic for Pneumology, Thoracic Oncology, Sleep, and Respiratory Critical Care, Klinikverbund Kempten-Oberallgaeu, Kempten and Immenstadt, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Schumann
authors.affiliation: Bayer Pharma AG, Berlin, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Krahn
authors.affiliation: Department of Tumor Biology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany. pantel@uke.de.
authors.forename: Klaus
authors.initials: K
authors.lastname: Pantel
mesh_terms: D000072283:A549 Cells; D002452:Cell Count / Q000379:methods; D045744:Cell Line, Tumor; D066246:ErbB Receptors / Q000378:metabolism; D006801:Humans; D008175:Lung Neoplasms / Q000097:blood* / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D009154:Mutation / Q000235:genetics; D009360:Neoplastic Cells, Circulating / Q000378:metabolism / Q000473:pathology*; D011446:Prospective Studies; D016283:Proto-Oncogene Proteins p21(ras) / Q000378:metabolism
chemical_list: D066246:ErbB Receptors; D016283:Proto-Oncogene Proteins p21(ras)