title: [The expression and significance of hnRNPD in esophageal squamous cell carcinoma cells].
abstract: OBJECTIVE
  To investigate the expression of heterogeneous nuclear ribonucleoprotein D (hnRNPD) in esophageal squamous cell carcinoma (ESCC) tissues and the relationship between hnRNPD expression and the clinicopathological features of ESCC, and to study the effect of down-regulated hnRNPD on the proliferation of ESCC cells and explore its potential mechanism.
  
  
  METHODS
  The expression of hnRNPD protein in ESCC tissues and the normal paracancerous tissues were detected by immunohistochemistry. The siRNA-hnRNPD was transfected into ESCC cells and the silence effect was verified by Western blotting. MTT assay and clone formation assay were used to evaluate the proliferation of ESCC cells after down-regulation of hnRNPD genes. Cell apoptosis was examined by annexin V-phycoerythrin/7-aminoactinomycin D (annexin V-PE/7-AAD) staining and flow cytometry.
  
  
  RESULTS
  The expression of hnRNPD protein in ESCC tissues was significantly higher than that of the normal paracancerous tissues, and the expression was closely related with neoplasm staging. Down-regulation of hnRNPD inhibited the proliferation and clonality of ESCC cells. Compared with the control group, siRNA targeting hnRNPD significantly promoted cell apoptosis.
  
  
  CONCLUSIONS
  Down-regulation of hnRNPD inhibits the proliferation of ESCC cells by promoting cell apoptosis.
authors.affiliation: School of Radiation Medicine and Protection, Medical College, Soochow University, Jiangsu Key Laboratory of Radiation Medicine and Protection, Suzhou 215123, China.
authors.forename: Yangyang
authors.initials: Y
authors.lastname: Geng
authors.affiliation: School of Radiation Medicine and Protection, Medical College, Soochow University, Jiangsu Key Laboratory of Radiation Medicine and Protection, Suzhou 215123, China.
authors.forename: Lulu
authors.initials: L
authors.lastname: Zhang
authors.affiliation: School of Radiation Medicine and Protection, Medical College, Soochow University, Jiangsu Key Laboratory of Radiation Medicine and Protection, Suzhou 215123, China.
authors.forename: Miaomiao
authors.initials: M
authors.lastname: Xu
authors.affiliation: School of Radiation Medicine and Protection, Medical College, Soochow University, Jiangsu Key Laboratory of Radiation Medicine and Protection, Suzhou 215123, China.
authors.forename: Wenjiong
authors.initials: W
authors.lastname: Sheng
authors.affiliation: School of Radiation Medicine and Protection, Medical College, Soochow University, Jiangsu Key Laboratory of Radiation Medicine and Protection, Suzhou 215123, China.
authors.forename: Aijing
authors.initials: A
authors.lastname: Dong
authors.affiliation: School of Radiation Medicine and Protection, Medical College, Soochow University, Jiangsu Key Laboratory of Radiation Medicine and Protection, Suzhou 215123, China.
authors.forename: Jinming
authors.initials: J
authors.lastname: Cao
authors.affiliation: School of Radiation Medicine and Protection, Medical College, Soochow University, Jiangsu Key Laboratory of Radiation Medicine and Protection, Suzhou 215123, China. *Corresponding author, E-mail: jpcao@suda.edu.cn.
authors.forename: Jianping
authors.initials: J
authors.lastname: Cao
mesh_terms: D000328:Adult; D000368:Aged; D017209:Apoptosis; D002294:Carcinoma, Squamous Cell / Q000201:enzymology* / Q000235:genetics / Q000503:physiopathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation; D004938:Esophageal Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000503:physiopathology; D000077277:Esophageal Squamous Cell Carcinoma; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D034502:Heterogeneous-Nuclear Ribonucleoprotein D / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged
chemical_list: D034502:Heterogeneous-Nuclear Ribonucleoprotein D