title: Transcriptome and proteome dynamics in larvae of the barnacle Balanus Amphitrite from the Red Sea.
abstract: BACKGROUND
  The barnacle Balanus amphitrite is widely distributed in marine shallow and tidal waters, and has significant economic and ecological importance. Nauplii, the first larval stage of most crustaceans, are extremely abundant in the marine zooplankton. However, a lack of genome information has hindered elucidation of the molecular mechanisms of development, settlement and survival strategies in extreme marine environments. We sequenced and constructed the genome dataset for nauplii to obtain comprehensive larval genetic information. We also investigated iTRAQ-based protein expression patterns to reveal the molecular basis of nauplii development, and to gain information on larval survival strategies in the Red Sea marine environment.
  
  
  RESULTS
  A nauplii larval transcript dataset, containing 92,117 predicted open reading frames (ORFs), was constructed and used as a reference for the proteome analysis. Genes related to translation, oxidative phosphorylation and cytoskeletal development were highly abundant. We observed remarkable plasticity in the proteome of Red Sea larvae. The proteins associated with development, stress responses and osmoregulation showed the most significant differences between the two larval populations studied. The synergistic overexpression of heat shock and osmoregulatory proteins may facilitate larval survival in intertidal habitats or in extreme environments.
  
  
  CONCLUSIONS
  We presented, for the first time, comprehensive transcriptome and proteome datasets for Red Sea nauplii. The datasets provide a foundation for future investigations focused on the survival mechanisms of other crustaceans in extreme marine environments.
authors.affiliation: KAUST Environmental Epigenetic Program (KEEP), Division of Biological and Environmental Sciences and Engineering, King Abdullah University of Science and Technology, Thuwal, Kingdom of Saudi Arabia. Kondethimmanahalli.Chandramouli@kaust.edu.sa.
authors.forename: Kondethimmanahalli H
authors.initials: KH
authors.lastname: Chandramouli
authors.affiliation: KAUST Environmental Epigenetic Program (KEEP), Division of Biological and Environmental Sciences and Engineering, King Abdullah University of Science and Technology, Thuwal, Kingdom of Saudi Arabia. sarah.aqeel@kaust.edu.sa.
authors.forename: Sarah
authors.initials: S
authors.lastname: Al-Aqeel
authors.affiliation: KAUST Environmental Epigenetic Program (KEEP), Division of Biological and Environmental Sciences and Engineering, King Abdullah University of Science and Technology, Thuwal, Kingdom of Saudi Arabia. taewoo.ryu@kaust.edu.sa.
authors.forename: Taewoo
authors.initials: T
authors.lastname: Ryu
authors.affiliation: Bioscience Core Laboratory, King Abdullah University of Science and Technology, Thuwal, Kingdom of Saudi Arabia. Huoming.Zhang@kaust.edu.sa.
authors.forename: Huoming
authors.initials: H
authors.lastname: Zhang
authors.affiliation: KAUST Environmental Epigenetic Program (KEEP), Division of Biological and Environmental Sciences and Engineering, King Abdullah University of Science and Technology, Thuwal, Kingdom of Saudi Arabia. loqmane.seridi@kaust.edu.sa.
authors.forename: Loqmane
authors.initials: L
authors.lastname: Seridi
authors.affiliation: KAUST Environmental Epigenetic Program (KEEP), Division of Biological and Environmental Sciences and Engineering, King Abdullah University of Science and Technology, Thuwal, Kingdom of Saudi Arabia. yanal.ghosheh@kaust.edu.sa.
authors.forename: Yanal
authors.initials: Y
authors.lastname: Ghosheh
authors.affiliation: KAUST Global Collaborative Research Program, Division of Life Science, The Hong Kong University of Science and Technology, Hong Kong, Hong Kong. boqianpy@ust.hk.
authors.forename: Pei-Yuan
authors.initials: PY
authors.lastname: Qian
authors.affiliation: KAUST Environmental Epigenetic Program (KEEP), Division of Biological and Environmental Sciences and Engineering, King Abdullah University of Science and Technology, Thuwal, Kingdom of Saudi Arabia. timothy.ravasi@kaust.edu.sa.|Division of Applied Mathematics and Computer Sciences, King Abdullah University of Science and Technology, Thuwal, Kingdom of Saudi Arabia. timothy.ravasi@kaust.edu.sa.
authors.forename: Timothy
authors.initials: T
authors.lastname: Ravasi
mesh_terms: D000818:Animals; D030541:Databases, Genetic; D020869:Gene Expression Profiling / Q000379:methods*; D018507:Gene Expression Regulation, Developmental; D019156:Indian Ocean; D007814:Larva / Q000235:genetics / Q000378:metabolism; D016366:Open Reading Frames; D040901:Proteomics / Q000379:methods*; D017422:Sequence Analysis, DNA; D001468:Thoracica / Q000235:genetics* / Q000378:metabolism*