title: An Investigation of Equine Mesenchymal Stem Cell Characteristics from Different Harvest Sites: More Similar Than Not.
abstract: Diseases of the musculoskeletal system are a major cause of loss of use and retirement in sport horses. The use of bone marrow-derived mesenchymal stem cells (BMDMSCs) for healing of traumatized tissue has gained substantial favor in clinical settings and can assist healing and tissue regeneration in orthopedic injuries. There are two common sites of harvest of BMDMSCs, the sternum and the ilium. Our objective was to determine if any differences exist in BMDMSCs acquired from the sternum and the ilium. We compared the two harvest sites in their propensity to undergo multilineage differentiation, differences in cell surface markers, or gene transduction efficiencies. BMDMSCs were isolated and culture-expanded from 5 ml aspirates of bone marrow from sternum and ilium. The cells were then plated and cultured with appropriate differentiation medium to result in multi-lineage differentiation and cell characteristics were compared between sternal and ilial samples. Cell surface antibody expression of CD11a/18, CD34, CD44, and CD90 were evaluated using flow cytometry, and gene transduction efficiencies were evaluated using GFP scAAV. There were no statistically significant differences in cell characteristics between MSCs cultured from the sternum and the ilium under any circumstances.
authors.affiliation: Gail Holmes Equine Orthopaedic Research Center, College of Veterinary Medicine and Biomedical Science, Colorado State University , Fort Collins, CO , USA.
authors.forename: Karla G
authors.initials: KG
authors.lastname: Lombana
authors.affiliation: Gail Holmes Equine Orthopaedic Research Center, College of Veterinary Medicine and Biomedical Science, Colorado State University , Fort Collins, CO , USA.
authors.forename: Laurie R
authors.initials: LR
authors.lastname: Goodrich
authors.affiliation: Gail Holmes Equine Orthopaedic Research Center, College of Veterinary Medicine and Biomedical Science, Colorado State University , Fort Collins, CO , USA.
authors.forename: Jennifer Nikki
authors.initials: JN
authors.lastname: Phillips
authors.affiliation: Gail Holmes Equine Orthopaedic Research Center, College of Veterinary Medicine and Biomedical Science, Colorado State University , Fort Collins, CO , USA.
authors.forename: John David
authors.initials: JD
authors.lastname: Kisiday
authors.affiliation: Department of Comparative Pathobiology, Purdue University , West Lafayette, IN , USA.
authors.forename: Audrey
authors.initials: A
authors.lastname: Ruple-Czerniak
authors.affiliation: Gail Holmes Equine Orthopaedic Research Center, College of Veterinary Medicine and Biomedical Science, Colorado State University , Fort Collins, CO , USA.
authors.forename: C Wayne
authors.initials: CW
authors.lastname: McIlwraith
keywords: gene therapy; horse; orthopedic; regeneration; stem cells; tendon