title: Advances in quantitative bioanalysis of oligonucleotide biomarkers and therapeutics.
abstract: Technical advances and demands for high-throughput accurate quantification of oligonucleotide therapeutics and biomarkers in pharmaceutical research and clinical diagnosis have aided evolution in quantitative bioanalysis of oligonucleotides. Many bioanalytical methods are available for absolute quantification of oligonucleotides in biological matrices. They can be broadly classified into two categories: hybridization-based assays commonly used by molecular biologists and chromatographic assays routinely used by chemists. Each category has its own advantages and disadvantages for specific applications. This review summarizes the mechanisms and applications of some of the current most commonly used techniques in each category.
authors.affiliation: NovaBioAssays LLC, 52 Dragon Court, Suite 3B, Woburn, MA 01801, USA.
authors.forename: Laixin
authors.initials: L
authors.lastname: Wang
authors.affiliation: NovaBioAssays LLC, 52 Dragon Court, Suite 3B, Woburn, MA 01801, USA.
authors.forename: Chengjie
authors.initials: C
authors.lastname: Ji
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D005821:Genetic Techniques; D006801:Humans; D009841:Oligonucleotides / Q000032:analysis* / Q000235:genetics / Q000627:therapeutic use*
chemical_list: D015415:Biomarkers; D009841:Oligonucleotides
keywords: LC-HRAM; LC-fluorescence; LC–MS/MS; dPCR; hybridization; oligonucleotide; qPCR; siRNA