title: Does the Capsule Interfere with Performance of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Identification of Cryptococcus neoformans and Cryptococcus gattii?
abstract: We described the impact of the capsule size for Cryptococcus neoformans and Cryptococcus gattii identification at the species level by Bruker matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). After experimental capsule size modulation, we observed that reducing the capsule size resulted in improved identification by Bruker MALDI-TOF MS across all of the reference strains analyzed.
authors.affiliation: Laboratory of Medical Mycology, LIM-53, Hospital das Clínicas FMUSP and Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, São Paulo, Brazil dan.yamamoto.thz@hotmail.com.
authors.forename: Danilo Y
authors.initials: DY
authors.lastname: Thomaz
authors.affiliation: Department of Biophysics, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, Brazil.
authors.forename: Rafaella C
authors.initials: RC
authors.lastname: Grenfell
authors.affiliation: Laboratory of Medical Mycology, LIM-53, Hospital das Clínicas FMUSP and Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, São Paulo, Brazil.
authors.forename: Monica S M
authors.initials: MS
authors.lastname: Vidal
authors.affiliation: Laboratory of Medical Mycology, LIM-53, Hospital das Clínicas FMUSP and Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, São Paulo, Brazil.
authors.forename: Mauro C
authors.initials: MC
authors.lastname: Giudice
authors.affiliation: Laboratory of Medical Mycology, LIM-53, Hospital das Clínicas FMUSP and Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, São Paulo, Brazil.
authors.forename: Gilda M B
authors.initials: GM
authors.lastname: Del Negro
authors.affiliation: Department of Biophysics, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, Brazil.
authors.forename: Luiz
authors.initials: L
authors.lastname: Juliano
authors.affiliation: Laboratory of Medical Mycology, LIM-53, Hospital das Clínicas FMUSP and Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, São Paulo, Brazil.
authors.forename: Gil
authors.initials: G
authors.lastname: Benard
authors.affiliation: Laboratory of Medical Mycology, LIM-53, Hospital das Clínicas FMUSP and Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, São Paulo, Brazil Central Laboratory Division, LIM-03, Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil.
authors.forename: João N
authors.initials: JN
authors.lastname: de Almeida Júnior
mesh_terms: D003453:Cryptococcosis / Q000175:diagnosis / Q000382:microbiology; D056285:Cryptococcus gattii / Q000145:classification* / Q000235:genetics; D003455:Cryptococcus neoformans / Q000145:classification* / Q000235:genetics; D005838:Genotype; D006801:Humans; D016533:Mycological Typing Techniques / Q000379:methods; D012680:Sensitivity and Specificity; D065288:Serogroup; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods