title: A comparative study of insertion/deletion polymorphisms applied among Southwest, South and Northwest Chinese populations using Investigator(®) DIPplex.
abstract: The low mutation rates, short amplicon sizes and length variation characteristics of biallelic insertion/deletion polymorphisms (INDELs) are features that allow combining advantages of both STR and SNP markers for genetic analysis. The Qiagen Investigator DIPplex kit contains 30 biallelic autosomal INDELs plus Amelogenin for forensic personal identification and parentage testing. This study compares general performances, forensic and population data collected in 7 Chinese ethnic populations (Han, Tibetan, Yi, Zhuang, Dong, Miao and Uyghur) from three areas (Southwest, South and Northwest) of China by DIPplex kit. There were no significant departures from Hardy-Weinberg equilibrium (HWE) of the 30 markers in all the tested populations after Bonferroni correction. The combined matching probabilities ranged from 1.4273×10(-10) for the Dong minority to 1.2817×10(-12) for the Uyghur minority. The combined power of paternity exclusion was from 0.9854 to 0.9968. All pairwise genetic differences (FST) between the seven ethnic groups were less than 0.05, and the results of STRUCTURE analysis did not indicate the presence of substructures suggesting genetic similarity among the populations in these three areas. The DIPplex kit can be used as a good additional tool for forensic personal identification in these three areas of China.
authors.affiliation: Institute of Forensic Medicine, West China School of Basic Science and Forensic Medicine, Sichuan University, Chengdu, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.affiliation: Institute of Forensic Medicine, West China School of Basic Science and Forensic Medicine, Sichuan University, Chengdu, PR China.
authors.forename: Meili
authors.initials: M
authors.lastname: Lv
authors.affiliation: Institute of Legal Medicine, Faculty of Medicine, University of Cologne, Cologne, Germany.
authors.forename: Daniel
authors.initials: D
authors.lastname: Zaumsegel
authors.affiliation: Department of Pathology and Pathophysiology, Chengdu Medical College, Chengdu 610500, China.
authors.forename: Lushun
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Leshan Public Security Bureau, Leshan, PR China.
authors.forename: Fengji
authors.initials: F
authors.lastname: Liu
authors.affiliation: Leshan Public Security Bureau, Leshan, PR China.
authors.forename: Jihua
authors.initials: J
authors.lastname: Xiang
authors.affiliation: Guangxi Institute of Criminal Science and Technology, Liuzhou Public Security Bureau, Liuzhou, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.affiliation: Institute of Legal Medicine, Faculty of Medicine, University of Cologne, Cologne, Germany.
authors.forename: Peter M
authors.initials: PM
authors.lastname: Schneider
authors.affiliation: Institute of Forensic Medicine, West China School of Basic Science and Forensic Medicine, Sichuan University, Chengdu, PR China; Institute of Legal Medicine, Faculty of Medicine, University of Cologne, Cologne, Germany. Electronic address: liangweibo@gmail.com.
authors.forename: Weibo
authors.initials: W
authors.lastname: Liang
authors.affiliation: Institute of Forensic Medicine, West China School of Basic Science and Forensic Medicine, Sichuan University, Chengdu, PR China. Electronic address: zhanglin@scu.edu.cn.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
mesh_terms: D044466:Asian People / Q000235:genetics*; D002681:China; D016172:DNA Fingerprinting / Q000379:methods*; D005006:Ethnicity / Q000235:genetics*; D005787:Gene Frequency; D005820:Genetic Testing; D005828:Genetics, Population; D006801:Humans; D054643:INDEL Mutation; D015810:Linkage Disequilibrium; D011110:Polymorphism, Genetic
keywords: China; DIPplex; F(ST); Forensic genetics; Insertion/deletion polymorphism (INDEL)