title: Changes in Lipid Composition During Manganese-Induced Apoptosis in PC12 Cells.
abstract: Lipid composition of membranes is fundamental to modulate signaling pathways relying on lipid metabolites and/or membrane proteins, thus resulting in the regulation of important cell processes such as apoptosis. In this case, membrane remodeling is an early event important for the activation of signaling leading to cell death and removal of apoptotic cells. In the present study, we analyzed phospholipid, cholesterol and fatty acid content during apoptosis induced by manganese in PC12 cells. Lipid analysis of whole cells and detergent-resistant membranes was carried out by HPLC/GC. Results showed that apoptosis is associated with changes in lipid composition detectable in whole cell extracts, namely cholesterol, phosphatidylserine and phosphatidylethanolamine decreases. Noteworthy, phosphatidylserine level reduction was detectable before to the detection of apoptosis, in correlation with our previous study carried out by radioactive labelling. By contrast, phosphatidylserine and phosphatidylethanolamine changes were not detected in detergent resistant membranes, which instead showed an altered composition in phosphatidylinositol, phosphatidylcholine and sphingomyelin in apoptotic cells.
authors.affiliation: Laboratory of Membrane Biochemistry and Applied Nutrition, Department of Pharmacological and Biomolecular Sciences, University of Milan, Via D. Trentacoste 2, 20134, Milan, Italy.
authors.forename: P A
authors.initials: PA
authors.lastname: Corsetto
authors.affiliation: Laboratory of Biochemistry and Molecular Biology, Department of Chemistry, Biology and Biotechnology, University of Perugia, Via del Giochetto, 06123, Perugia, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Ferrara
authors.affiliation: Laboratory of Biochemistry and Molecular Biology, Department of Chemistry, Biology and Biotechnology, University of Perugia, Via del Giochetto, 06123, Perugia, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Buratta
authors.affiliation: Laboratory of Biochemistry and Molecular Biology, Department of Chemistry, Biology and Biotechnology, University of Perugia, Via del Giochetto, 06123, Perugia, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Urbanelli
authors.affiliation: Laboratory of Membrane Biochemistry and Applied Nutrition, Department of Pharmacological and Biomolecular Sciences, University of Milan, Via D. Trentacoste 2, 20134, Milan, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Montorfano
authors.affiliation: Department of Medicine, School of Medicine, University of Perugia, Perugia, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Gambelunghe
authors.affiliation: Department of Veterinary Medicine, University of Perugia, Via San Costanzo 4, 06126, Perugia, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Chiaradia
authors.affiliation: Laboratory of Biochemistry and Molecular Biology, Department of Chemistry, Biology and Biotechnology, University of Perugia, Via del Giochetto, 06123, Perugia, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Magini
authors.affiliation: Laboratory of Membrane Biochemistry and Applied Nutrition, Department of Pharmacological and Biomolecular Sciences, University of Milan, Via D. Trentacoste 2, 20134, Milan, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Roderi
authors.affiliation: Laboratory of Membrane Biochemistry and Applied Nutrition, Department of Pharmacological and Biomolecular Sciences, University of Milan, Via D. Trentacoste 2, 20134, Milan, Italy.
authors.forename: I
authors.initials: I
authors.lastname: Colombo
authors.affiliation: Laboratory of Membrane Biochemistry and Applied Nutrition, Department of Pharmacological and Biomolecular Sciences, University of Milan, Via D. Trentacoste 2, 20134, Milan, Italy. angelamaria.rizzo@unimi.it.
authors.forename: A M
authors.initials: AM
authors.lastname: Rizzo
authors.affiliation: Laboratory of Biochemistry and Molecular Biology, Department of Chemistry, Biology and Biotechnology, University of Perugia, Via del Giochetto, 06123, Perugia, Italy. carla.emiliani@unipg.it.|CEMIN-Center of Excellence for Innovative Nanostructured Material, Perugia, Italy. carla.emiliani@unipg.it.
authors.forename: C
authors.initials: C
authors.lastname: Emiliani
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002712:Chlorides / Q000008:administration & dosage; D050356:Lipid Metabolism; D008345:Manganese / Q000494:pharmacology*; D017895:Manganese Compounds / Q000008:administration & dosage; D016716:PC12 Cells; D010743:Phospholipids / Q000737:chemistry; D051381:Rats; D015398:Signal Transduction
chemical_list: D002712:Chlorides; D017895:Manganese Compounds; D010743:Phospholipids; D008345:Manganese; C025340:manganese chloride
keywords: Apoptosis; Detergent-resistant membranes; Manganese; PC12 cells; Phosphatidylserine; Phospholipid composition