title: EPITOPE-BASED VACCINE FOR THE TREATMENT OF DER F 3 ALLERGY.
abstract: BACKGROUND
  mites allergic asthma is caused by exposure to home dust mite (HDM). Der f 3 is believed to be one of the major allergens in mites allergic asthma. The work was to identify the immune characteristics of Der f 3 epitope-based vaccine containing T cell and B cell epitopes.
  
  
  METHODS
  T cell lines were generated from peripheral blood mononuclear cells of Der f 3 allergic patients. Three T cell epitopes and five B cell epitopes of Der f 3, which we identified previously, were selected to design a polypeptide (named Der f3-peptides). DNA constructions encoding these Der f 3-peptides were expressed in Escherichia coli. The T cell lines were stimulated with the peptides and tested for proliferative capacity and cytokine production.
  
  
  RESULTS
  plasmid pET28a (+)-Der f 3-peptides was constructed and expressed in E. coli BL21, and the Der f3-peptides protein was purified and confirmed by Western blotting. The Der f 3-peptides were recognized by the T cell clones from allergic patients. SI value of Der f 3 group and Der f 3-peptides group were both higher than that of PBS group (P<0.05). The Der f 3 and Der f 3 peptides induced secretions of IL-4 and IL-5 were decreased compared with that of PBS group (P<0.05). The capacity of IgE-binding to Der f 3-peptides (41.25±5.67) μg/ml was decreased dramatically compared with that of Der f 3 (83.60 ± 10.92) μg/ml (P < 0.05).
  
  
  CONCLUSIONS
  our results demonstrate that several major T cell epitopes and B cell epitopes of Der f 3 can be valuable for designing the peptide-based immunotherapeutics for the mites allergic asthma.
authors.affiliation: Deparment of Medical Parasitology, Wannan Medical College, Wuhu 241002, Anhui, People's Republic of China. College of life science, Anhui Normal University, Wuhu 241002, Anhui, People's Republic of China.. xdzhan@126.com.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Zhan
authors.affiliation: Deparment of Medical Parasitology, Wannan Medical College, Wuhu 241002, Anhui, People's Republic of China.. cpli001@126.com.
authors.forename: Chaopin
authors.initials: C
authors.lastname: Li
authors.affiliation: Deparment of Medical Parasitology, Wannan Medical College, Wuhu 241002, Anhui, People's Republic of China.. jiangyuxin2000@hotmail.com.
authors.forename: Yuxing
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Deparment of Medical Parasitology, Wannan Medical College, Wuhu 241002, Anhui, People's Republic of China.. 1129910161@qq.com.
authors.forename: Na
authors.initials: N
authors.lastname: Li
mesh_terms: D000328:Adult; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D016207:Cytokines / Q000096:biosynthesis; D018984:Epitopes, T-Lymphocyte / Q000276:immunology*; D005260:Female; D006801:Humans; D008297:Male; D039981:Pyroglyphidae / Q000276:immunology*; D012130:Respiratory Hypersensitivity / Q000517:prevention & control*; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D014612:Vaccines / Q000276:immunology* / Q000627:therapeutic use*; D014614:Vaccines, Synthetic / Q000276:immunology; D055815:Young Adult
chemical_list: D016207:Cytokines; D018984:Epitopes, T-Lymphocyte; D014612:Vaccines; D014614:Vaccines, Synthetic