title: [Effect of silicon dioxide nanoparticles on expression and DNA methylation of PARP-1 gene in HaCaT cells].
abstract: OBJECTIVE
  To study the effect of silicon dioxide nanoparticles on the expression and promoter region CpG islands methylation of (Poly [ADP-ribose] polymerase 1, PARP-1) gene in human HaCaT Cell.
  
  
  METHODS
  HaCaT Cells were treated with nm-SiO₂at 0, 2.5, 5 and 10 µg/mL and micro-SiO₂at 10 µg/ml for 24 h and DAC treatment was given at 10 µg/ml group for 48 h. Real-time PCR and western blot assay was used to detect the expression of PARP-1 mRNA and protein. BSP (Bisulfite Pyrosequence, BSP) assay was used to detect the promoter region CpG islands methylation status of PARP-1 gene.
  
  
  RESULTS
  After exposure to nano-SiO₂particles, compared to CTRL group, the mRNA and protein expression of PARP-1 in micro-SiO₂and 2.5 µg/ml group unchanged, but he mRNA and protein expression of PARP-1 in 5, 10 µg/ml as well as DAC group was down-regulated and there are statistical significance between CTRL group and 5, 10 µg/ml as well as DAC group and the PARP-1 promoter region CpG islands showed methylation.
  
  
  CONCLUSIONS
  nano-SiO₂can down-regulate PARP-1 expression in HaCaT Cell and this is associated with the change in the methylation of PARP-1 gene promoter region CpG islands induced by nano-SiO₂particles.
authors.affiliation: Shenzhen Center for Chronic Disease Control, Shenzhen 518020, China.
authors.forename: Chunmei
authors.initials: C
authors.lastname: Gong
authors.forename: Linqing
authors.initials: L
authors.lastname: Yang
authors.forename: Jichang
authors.initials: J
authors.lastname: Zhou
authors.forename: Gonghua
authors.initials: G
authors.lastname: Tao
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Liu
authors.affiliation: E-mail: zxzhuang2007@126.com.
authors.forename: Zhixiong
authors.initials: Z
authors.lastname: Zhuang
mesh_terms: D045744:Cell Line, Tumor; D018899:CpG Islands; D019175:DNA Methylation; D006801:Humans; D053758:Nanoparticles / Q000009:adverse effects*; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000378:metabolism; D012822:Silicon Dioxide / Q000009:adverse effects*
chemical_list: D012333:RNA, Messenger; D012822:Silicon Dioxide; C499320:PARP1 protein, human; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases