title: Left-right asymmetric cell intercalation drives directional collective cell movement in epithelial morphogenesis.
abstract: Morphogenetic epithelial movement occurs during embryogenesis and drives complex tissue formation. However, how epithelial cells coordinate their unidirectional movement while maintaining epithelial integrity is unclear. Here we propose a novel mechanism for collective epithelial cell movement based on Drosophila genitalia rotation, in which epithelial tissue rotates clockwise around the genitalia. We found that this cell movement occurs autonomously and requires myosin II. The moving cells exhibit repeated left-right-biased junction remodelling, while maintaining adhesion with their neighbours, in association with a polarized myosin II distribution. Reducing myosinID, known to cause counter-clockwise epithelial-tissue movement, reverses the myosin II distribution. Numerical simulations revealed that a left-right asymmetry in cell intercalation is sufficient to induce unidirectional cellular movement. The cellular movement direction is also associated with planar cell-shape chirality. These findings support a model in which left-right asymmetric cell intercalation within an epithelial sheet drives collective cellular movement in the same direction.
authors.affiliation: Laboratory for Physical Biology, RIKEN Center for Developmental Biology, Kobe 650-0047, Japan.
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Sato
authors.affiliation: Laboratory for Physical Biology, RIKEN Center for Developmental Biology, Kobe 650-0047, Japan.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Hiraiwa
authors.affiliation: Laboratory for Histogenetic Dynamics, RIKEN Center for Developmental Biology, Kobe 650-0047, Japan.
authors.forename: Emi
authors.initials: E
authors.lastname: Maekawa
authors.affiliation: Laboratory for Histogenetic Dynamics, RIKEN Center for Developmental Biology, Kobe 650-0047, Japan.
authors.forename: Ayako
authors.initials: A
authors.lastname: Isomura
authors.affiliation: Laboratory for Physical Biology, RIKEN Center for Developmental Biology, Kobe 650-0047, Japan.
authors.forename: Tatsuo
authors.identifier: http://orcid.org/0000-0002-9294-9998
authors.initials: T
authors.lastname: Shibata
authors.affiliation: Laboratory for Histogenetic Dynamics, RIKEN Center for Developmental Biology, Kobe 650-0047, Japan.|Laboratory of Molecular Cell Biology and Development, Graduate School of Biostudies, Kyoto University, Kyoto 606-8501, Japan.|Laboratory for Tissue Development Dynamics, Graduate School of Biological Sciences, Nara Institute of Science and Technology, Nara 630-0192, Japan.|Laboratory for Histogenetic Dynamics, Graduate School of Life Sciences, Tohoku University, Sendai 980-8577, Japan.
authors.forename: Erina
authors.initials: E
authors.lastname: Kuranaga
mesh_terms: D000818:Animals; D019521:Body Patterning / Q000502:physiology*; D002465:Cell Movement; D016764:Cell Polarity; D048430:Cell Shape; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000254:growth & development* / Q000502:physiology*; D004847:Epithelial Cells / Q000166:cytology* / Q000502:physiology; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D008297:Male; D009024:Morphogenesis; D011679:Pupa
chemical_list: D029721:Drosophila Proteins