title: Postsynthetic Modification of DNA Phosphodiester Backbone for Photocaged DNAzyme.
abstract: Photocaged (photoactivatable) biomolecules are powerful tools for noninvasive control of biochemical activities by light irradiation. DNAzymes (deoxyribozymes) are single-stranded oligonucleotides with a broad range of enzymatic activities. In this work, to construct photocaged DNAzymes, we developed a facile and mild postsynthetic method to incorporate an interesting photolabile modification (thioether-enol phosphate, phenol substituted, TEEP-OH) into readily available phosphorothioate DNA. Upon light irradiation, TEEP-OH transformed into a native DNA phosphodiester, and accordingly the DNAzymes with RNA-cleaving activities were turned "on" from its inactive and caged form. Activation of the TEEP-OH-caged DNAzyme by light was also successful inside live cells.
authors.affiliation: Department of Chemistry, Beijing Key Laboratory for Microanalytical Methods and Instrumentation, Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology (Ministry of Education), Tsinghua University , Beijing 100084, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Chemistry, Beijing Key Laboratory for Microanalytical Methods and Instrumentation, Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology (Ministry of Education), Tsinghua University , Beijing 100084, China.
authors.forename: Mengli
authors.initials: M
authors.lastname: Feng
authors.affiliation: Department of Chemistry, Beijing Key Laboratory for Microanalytical Methods and Instrumentation, Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology (Ministry of Education), Tsinghua University , Beijing 100084, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Xiao
authors.affiliation: Department of Chemistry, Beijing Key Laboratory for Microanalytical Methods and Instrumentation, Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology (Ministry of Education), Tsinghua University , Beijing 100084, China.
authors.forename: Aijun
authors.initials: A
authors.lastname: Tong
authors.affiliation: Department of Chemistry, Beijing Key Laboratory for Microanalytical Methods and Instrumentation, Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology (Ministry of Education), Tsinghua University , Beijing 100084, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Xiang
mesh_terms: D001483:Base Sequence; D021881:DNA, Catalytic / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D007700:Kinetics; D008958:Models, Molecular; D010636:Phenols / Q000737:chemistry; D054735:Phosphorothioate Oligonucleotides / Q000737:chemistry*; D012313:RNA / Q000737:chemistry*; D059367:RNA Cleavage / Q000528:radiation effects; D013440:Sulfides / Q000737:chemistry*; D014466:Ultraviolet Rays
chemical_list: D021881:DNA, Catalytic; D010636:Phenols; D054735:Phosphorothioate Oligonucleotides; D013440:Sulfides; D012313:RNA