title: Dynamics of the Plant Nuclear Envelope During Cell Division.
abstract: The use of suspension cultures synchronised by aphidicolin provides a method to study cell division in living plant cells. This chapter describes the use of this technique in tobacco suspension cultures expressing nuclear and nuclear envelope proteins that have been fused to fluorescent proteins. The protocol provides advice on optimizing synchrony and on real-time imaging by confocal microscopy.
authors.affiliation: Department of Biological and Medical Sciences, Faculty of Health and Life Sciences, Oxford Brookes University, Headington Campus, Oxford, OX3 0BP, UK. deevans@brookes.ac.uk.
authors.forename: David E
authors.initials: DE
authors.lastname: Evans
authors.affiliation: Department of Biological and Medical Sciences, Faculty of Health and Life Sciences, Oxford Brookes University, Oxford, UK. kgraumann@brookes.ac.uk.
authors.forename: Katja
authors.initials: K
authors.lastname: Graumann
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics; D018929:Cell Culture Techniques / Q000379:methods; D002455:Cell Division; D002460:Cell Line; D018613:Microscopy, Confocal / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D008938:Mitosis; D009685:Nuclear Envelope / Q000235:genetics / Q000648:ultrastructure*; D014026:Nicotiana / Q000166:cytology* / Q000235:genetics; D014170:Transformation, Genetic
keywords: Cell division; Cell synchrony; Mitosis; Nicotiana tabacum; Nuclear envelope; Nuclear envelope markers; Plant cell culture; Stable expression; Tobacco BY-2 cells; Transient expression