title: Intraspecific differentiation of Hancornia speciosa revealed by simple sequence repeat and random amplified polymorphic DNA markers.
abstract: Hancornia speciosa, popularly known as mangabeira, is a fruit tree native to the Brazilian Cerrado that shows great economic potential, due to its multiple uses. Intraspecific classification of this species is difficult because it shows high morphological diversity. An early study of the species reported that there are six botanic varieties that differ morphologically mainly in the shapes of their leaves and flowers. Except to note the wide morphological variation and economic potential of this species, few studies have been published about the genetic diversity of mangabeira. Knowledge of the genetic variability of this species among populations would be useful for genetic conservation and breeding programs. Therefore, we tested the transferability of 12 simple sequence repeats from expressed sequence tags (EST-SSRs) from Catharanthus roseus to H. speciosa and used 10 random amplified polymorphic DNA markers to evaluate the genetic variability among botanical varieties of H. speciosa. We obtained a high transferability frequency of EST-SSR markers from C. roseus to H. speciosa (75%). However, EST-SSR markers showed low heterozygosity and locus variability (two or three alleles by locus), which suggest low genetic diversity in the mangabeira samples. The Jaccard dissimilarity index and an examination of geographic distances indicated a non-spatial structuring of the genetic variability. Our markers were unable to distinguish H. speciosa botanical varieties.
authors.affiliation: Universidade Estadual de Goiás, Campus Anápolis de Ciências Exatas e Tecnológicas, Anápolis, GO, Brasil.
authors.forename: C A
authors.initials: CA
authors.lastname: Nogueira
authors.affiliation: Departamento de Ciências Exatas, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual de São Paulo "Júlio de Mesquita Filho", Jaboticabal, SP, Brasil.
authors.forename: N B
authors.initials: NB
authors.lastname: Stafuzza
authors.affiliation: Universidade Estadual de Goiás, Campus Ipameri, Ipameri, GO, Brasil.
authors.forename: T P
authors.initials: TP
authors.lastname: Ribeiro
authors.affiliation: Universidade Estadual de Goiás, Campus Ipameri, Ipameri, GO, Brasil.
authors.forename: A D L
authors.initials: AD
authors.lastname: Prado
authors.affiliation: Laboratório de Genética e Biologia Molecular, Instituto Federal Goiano, Urutaí, GO, Brasil.
authors.forename: I P P
authors.initials: IP
authors.lastname: Menezes
authors.affiliation: Universidade Estadual de Goiás, Campus Ipameri, Ipameri, GO, Brasil.
authors.forename: N
authors.initials: N
authors.lastname: Peixoto
authors.affiliation: Instituto de Física, Universidade Federal de Goiás, Goiânia, GO, Brasil.
authors.forename: P J
authors.initials: PJ
authors.lastname: Gonçalves
authors.affiliation: Universidade Estadual de Goiás, Campus Anápolis de Ciências Exatas e Tecnológicas, Anápolis, GO, Brasil.
authors.forename: L M
authors.initials: LM
authors.lastname: Almeida
mesh_terms: D001947:Breeding; D005819:Genetic Markers; D005828:Genetics, Population; D018895:Microsatellite Repeats; D010641:Phenotype; D010802:Phylogeny; D058974:Phylogeography; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique; D013045:Species Specificity; D064028:Tracheophyta / Q000145:classification* / Q000235:genetics*
chemical_list: D005819:Genetic Markers