title: Detection of blaPER-1 & blaOxa10 among imipenem resistant isolates of Pseudomonas aeruginosa isolated from burn patients hospitalized in Shiraz Burn Hospital.
abstract: OBJECTIVE
  Pseudomonas aeruginosa is one of the most important Gram negative opportunistic bacteria which causes infection among burn patients. Resistance to the antibiotics in this group of bacteria is increased due to the activity of extended spectrum β-lactamase (ESBLs) genes. In the current study, we investigated the prevalence of two genes (blaPER-1 & blaOxa10 ) related β-lactamase genes among imipenem resistance clinical isolates of P. aeruginosa in hospitalized patients.
  
  
  METHODS
  From May 2010 to March 2011, 270 P. aeruginosa isolated from hospitalized burned patients' wounds in Shiraz Burn Hospital, were tested for Imipenem resistance by disk diffusion method. Presence of ESBLs exo-enzyme, blaPER-1 and blaOxa10 genes were also evaluated in the resistant isolate.
  
  
  RESULTS
  210 (77.7%) of 270 P. aeruginosa isolates were resistant to imipenem. blaPER-1 and blaOxa10 were detected among 168 (80.0%) of imipenem resistant isolates. Furthermore, 160 (76.2%) of them had blaOxa10 gene and 84 (40.0%) of them had blaPER-1 while 63 (30.0%) resistant isolates contained both genes simultaneously.
  
  
  CONCLUSIONS
  This study showed a high prevalence of blaPER-1 and blaOxa10 genes in hospitalized burn patients in south west of Iran. Therefore, it's highly recommended to perform such tests routinely to evaluate the resistance pattern in order to better antibiotic selection in the burned patients.
authors.affiliation: Shiraz Burn Research Center, Department of Bacteriology and Virology, Shiraz University of Medical Sciences, Shiraz, Iran. ; Department of Bacteriology and Virology, Shiraz Medical School, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Amir
authors.initials: A
authors.lastname: Emami
authors.affiliation: Department of Bacteriology and Virology, Shiraz Medical School, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Abdollah
authors.initials: A
authors.lastname: Bazargani
authors.affiliation: Shiraz Burn Research Center, Division of plastic and reconstructive Surgery, Department of Surgery, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Ali Akbar
authors.initials: AA
authors.lastname: Mohammadi
authors.affiliation: Shiraz Burn Research Center, Division of plastic and reconstructive Surgery, Department of Surgery, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Mitra
authors.initials: M
authors.lastname: Zardosht
authors.affiliation: Shiraz Burn Research Center, Division of plastic and reconstructive Surgery, Department of Surgery, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Seyed Morteza
authors.initials: SM
authors.lastname: Seyed Jafari
keywords: Burn; ESBLs; Pseudomonas aeruginosa; Resistance; blaOxa10; blaPER-1