title: Suppression of NYVAC Infection in HeLa Cells Requires RNase L but Is Independent of Protein Kinase R Activity.
abstract: Protein kinase R (PKR) and RNase L are host cell components that function to contain viral spread after infections. In this study, we analyzed the role of both proteins in the abortive infection of human HeLa cells with the poxvirus strain NYVAC, for which an inhibition of viral A27L and B5R gene expression is described. Specifically, the translation of these viral genes is independent of PKR activation, but their expression is dependent on the RNase L activity.
authors.affiliation: Department of Preventive Medicine, Public Health and Microbiology, Universidad Autónoma, Madrid, Spain.
authors.forename: Mercedes
authors.initials: M
authors.lastname: Fernández-Escobar
authors.affiliation: Department of Molecular and Cell Biology, Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas, Campus Universidad Autónoma, Madrid, Spain.
authors.forename: José Luis
authors.initials: JL
authors.lastname: Nájera
authors.affiliation: Department of Preventive Medicine, Public Health and Microbiology, Universidad Autónoma, Madrid, Spain.
authors.forename: Sara
authors.initials: S
authors.lastname: Baldanta
authors.affiliation: Department of Molecular and Cell Biology, Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas, Campus Universidad Autónoma, Madrid, Spain.
authors.forename: Dolores
authors.initials: D
authors.lastname: Rodriguez
authors.affiliation: Cellular Signalling and Cytoskeletal Function, Francis Crick Institute, London, United Kingdom.
authors.forename: Michael
authors.initials: M
authors.lastname: Way
authors.affiliation: Department of Molecular and Cell Biology, Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas, Campus Universidad Autónoma, Madrid, Spain.
authors.forename: Mariano
authors.initials: M
authors.lastname: Esteban
authors.affiliation: Department of Preventive Medicine, Public Health and Microbiology, Universidad Autónoma, Madrid, Spain susana.guerra@uam.es.
authors.forename: Susana
authors.initials: S
authors.lastname: Guerra
mesh_terms: D004722:Endoribonucleases / Q000378:metabolism*; D004847:Epithelial Cells / Q000276:immunology*; D006367:HeLa Cells; D054884:Host-Pathogen Interactions; D006801:Humans; D008562:Membrane Glycoproteins / Q000037:antagonists & inhibitors*; D014176:Protein Biosynthesis; D014616:Vaccinia virus / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000037:antagonists & inhibitors*; D014764:Viral Proteins / Q000037:antagonists & inhibitors*; D019892:eIF-2 Kinase / Q000378:metabolism*
chemical_list: C110360:A27L protein, Orthopoxvirus; D008562:Membrane Glycoproteins; D014759:Viral Envelope Proteins; D014764:Viral Proteins; C074512:42kDa protein, Vaccinia virus; D019892:eIF-2 Kinase; D004722:Endoribonucleases; C031956:2-5A-dependent ribonuclease