title: [EFFECT OF LIPOPOLYSACCHARIDE ON NEUTRAL LIPID METABOLISM AND CELLULAR ENERGETICS IN FROG URINARY BLADDER EPITHELIAL CELLS].
abstract: It was shown previously that colonization of the frog urinary bladder by gram-negative bacteria leads to decreased ability of antidiuretic hormone to reabsorb water from the urinary bladder (Fock et al. J. Exp. Zool., 2013, 319A: 487-494). In the present work performed on epithelial cells isolated from the frog urinary bladder the influence of E. coli lipopolysaccharide (LPS) on neutral lipid metabolism and cellular energetics was studied. It was shown that incubation of cells with LPS led to decrease of fatty acids oxidation and to retention of triacylglycerols (TAG) followed by an increase of the cytoplasmic lipid droplets content and cellular amount of TAG. Fatty acid composition of TAG was not changed under LPS. LPS did not alter mitochondrial membrane potential, however, LPS decreased oxygen consumption rate both in basal and uncoupling conditions. Cellular ATP production was also reduced in the presence of LPS. The data obtained indicate that a decreased ability of antidiuretic hormone to reabsorb water from the urinary bladder induced by bacterial pathogens could be related to inhibition of fatty acids oxidation and impaired energy metabolism.
authors.forename: E V
authors.initials: EV
authors.lastname: Fedorova
authors.forename: E M
authors.initials: EM
authors.lastname: Fock
authors.forename: I V
authors.initials: IV
authors.lastname: Braylovskaya
authors.forename: V T
authors.initials: VT
authors.lastname: Bachteeva
authors.forename: E A
authors.initials: EA
authors.lastname: Lavrova
authors.forename: S A
authors.initials: SA
authors.lastname: Zabelinskiĭ
authors.forename: R G
authors.initials: RG
authors.lastname: Parnova
mesh_terms: D000255:Adenosine Triphosphate / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D000818:Animals; D004734:Energy Metabolism / Q000187:drug effects*; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D005227:Fatty Acids / Q000037:antagonists & inhibitors / Q000378:metabolism; D066292:Lipid Droplets / Q000187:drug effects / Q000378:metabolism; D050356:Lipid Metabolism / Q000187:drug effects*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D010101:Oxygen Consumption / Q000187:drug effects; D061251:Primary Cell Culture; D011896:Rana temporaria; D014280:Triglycerides / Q000819:agonists / Q000378:metabolism; D001743:Urinary Bladder / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D019459:Urothelium / Q000166:cytology / Q000187:drug effects / Q000378:metabolism
chemical_list: D005227:Fatty Acids; D008070:Lipopolysaccharides; D014280:Triglycerides; D000255:Adenosine Triphosphate