title: Esterification of oleic acid with methanol by immobilized lipase on wrinkled silica nanoparticles with highly ordered, radially oriented mesochannels.
abstract: Mesoporous silica nanoparticles with a wrinkled structure (wrinkled silica nanoparticles, WSNs) having highly ordered, radially oriented mesochannels were synthesized by a solvothermal method. The method used a mixture of cyclohexane, ethanol, and water as solvent, tetraethoxysilane (TEOS) as source of inorganic silica, ammonium hydroxide as hydrolysis additive, cetyltrimethylammonium bromide (CTAB) as surfactant, and polyvinylpyrrolidone (PVP) as stabilizing agent of particle growth. Particle size (240nm to 540nm), specific surface areas (490m(2)g(-1) to 634m(2)g(-1)), surface morphology (radial wrinkled structures), and pore structure (radially oriented mesochannels) of WSN samples were varied using different molar ratios of CTAB to PVP. Using synthesized WSN samples with radially oriented mesochannels as support, we prepared immobilized Candida rugosa lipase (CRL) as a new biocatalyst for biodiesel production through the esterification of oleic acid with methanol. These results suggest that WSNs with highly ordered, radially oriented mesochannels have promising applications in biocatalysis, with the highest oleic acid conversion rate of about 86.4% under the optimum conditions.
authors.affiliation: Key Laboratory of Fine Chemicals in Universities of Shandong, School of Chemistry and Pharmaceutical Engineering, Qilu University of Technology, Jinan 250353, PR China.
authors.forename: Jinli
authors.initials: J
authors.lastname: Pang
authors.affiliation: Key Laboratory of Fine Chemicals in Universities of Shandong, School of Chemistry and Pharmaceutical Engineering, Qilu University of Technology, Jinan 250353, PR China. Electronic address: guoweizhou@hotmail.com.
authors.forename: Guowei
authors.initials: G
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Fine Chemicals in Universities of Shandong, School of Chemistry and Pharmaceutical Engineering, Qilu University of Technology, Jinan 250353, PR China.
authors.forename: Ruirui
authors.initials: R
authors.lastname: Liu
authors.affiliation: Key Laboratory of Fine Chemicals in Universities of Shandong, School of Chemistry and Pharmaceutical Engineering, Qilu University of Technology, Jinan 250353, PR China.
authors.forename: Tianduo
authors.initials: T
authors.lastname: Li
mesh_terms: D002175:Candida / Q000201:enzymology*; D004800:Enzymes, Immobilized / Q000737:chemistry; D004951:Esterification; D005656:Fungal Proteins / Q000737:chemistry*; D008049:Lipase / Q000737:chemistry*; D000432:Methanol / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D019301:Oleic Acid / Q000737:chemistry*; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D019301:Oleic Acid; D012822:Silicon Dioxide; D008049:Lipase; D000432:Methanol
keywords: Biocatalysis; Biodiesel; Immobilized lipase; Radially oriented mesochannels; Wrinkled silica