title: [Spermatozoal immobilization ability and virulence genes of Staphylococcus aureus isolated from the semen of infertile men].
abstract: OBJECTIVE
  To investigate the impact of Staphylococcus aureus from infertile men on sperm motility and the relationship between virulence genes and the activity of spermatozoal immobilization.
  
  
  METHODS
  We collected 60 strains of non-repeated Staphylococcus aureus from the semen of 589 infertile males and analyzed the influence of Staphylococcus aureus on sperm motility using the computer-aided sperm analysis system. We selected the strains that apparently decreased sperm motility and detected their virulence genes by PCR.
  
  
  RESULTS
  Sperm motility was significantly decreased in 17 of the 60 strains of Staphylococcus aureus (P < 0.05). The main virulence genes in these strains were hlg (33.3%), scn (23.3%), cna (20%), hlb (20%), and clfA (18.3%), others including icaA, fnbA, tst, seb, hld, eta and sea. The scn gene carriers accounted for 47.1% in the spermatozal immobilization positive group, significantly higher than 14% in the negative group (P < 0.05). No statistically significant differences were found in the percentages of the carriers of the other virulence genes between the two groups (P > 0.05).
  
  
  CONCLUSIONS
  Infections of Staphylococcus aureus in male reproductive system can lead to the decrease of sperm motility, which may be associated with the Staphylococcus complement inhibitor encoding gene scn.
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.forename: Xu
authors.initials: X
authors.lastname: Yang
authors.forename: Jian-zhong
authors.initials: JZ
authors.lastname: Ye
authors.forename: Hua-le
authors.initials: HL
authors.lastname: Chen
authors.forename: Yuan-bo
authors.initials: YB
authors.lastname: Hou
authors.forename: Jia
authors.initials: J
authors.lastname: Du
authors.forename: Tie-li
authors.initials: TL
authors.lastname: Zhou
mesh_terms: D006801:Humans; D007248:Infertility, Male / Q000382:microbiology; D008297:Male; D016133:Polymerase Chain Reaction; D012661:Semen / Q000382:microbiology; D013045:Species Specificity; D013081:Sperm Motility; D013203:Staphylococcal Infections; D013211:Staphylococcus aureus / Q000472:pathogenicity*; D014774:Virulence / Q000235:genetics