title: Micropatterning Extracellular Matrix Proteins on Electrospun Fibrous Substrate Promote Human Mesenchymal Stem Cell Differentiation Toward Neurogenic Lineage.
abstract: In this study, hybrid micropatterned grafts constructed via a combination of microcontact printing and electrospinning techniques process were utilized to investigate the influencing of patterning directions on human mesenchymal stem cells (hMSCs) differentiation to desired phenotypes. We found that the stem cells could align and elongate along the direction of the micropattern, where they randomly distributed on nonmicropatterned surfaces. Concomitant with patterning effect of component on stem cell alignment, a commensurate increase on the expression of neural lineage commitment markers, such as microtubule associated protein 2 (MAP2), Nestin, NeuroD1, and Class III β-Tubulin, were revealed from mRNA expression by quantitative Real Time PCR (qRT-PCR) and MAP2 expression by immunostaining. In addition, the effect of electrospun fiber orientation on cell behaviors was further examined. An angle of 45° between the direction of micropatterning and orientation of aligned fibers was verified to greatly prompt the outgrowth of filopodia and neurogenesis of hMSCs. This study demonstrates that the significance of hybrid components and electrospun fiber alignment in modulating cellular behavior and neurogenic lineage commitment of hMSCs, suggesting promising application of porous scaffolds with smart component and topography engineering in clinical regenerative medicine.
authors.affiliation: Institute of Biomaterials and Engineering, Wenzhou Medical University , Chashan Higher Education Zone, Wenzhou 325035, China.|Wenzhou Institute of Biomaterials and Engineering, Chinese Academy of Sciences , 16 Xinsan Road, Wenzhou 325011, China.|School of Materials Science and Engineering, Nanyang Technological University , 50 Nanyang Avenue, 639798, Singapore.
authors.forename: Huaqiong
authors.initials: H
authors.lastname: Li
authors.affiliation: School of Materials Science and Engineering, Nanyang Technological University , 50 Nanyang Avenue, 639798, Singapore.
authors.forename: Feng
authors.initials: F
authors.lastname: Wen
authors.affiliation: School of Materials Science and Engineering, Nanyang Technological University , 50 Nanyang Avenue, 639798, Singapore.
authors.forename: Huizhi
authors.initials: H
authors.lastname: Chen
authors.affiliation: School of Materials Science and Engineering, Nanyang Technological University , 50 Nanyang Avenue, 639798, Singapore.
authors.forename: Mintu
authors.initials: M
authors.lastname: Pal
authors.affiliation: National Engineering Laboratory of Modern Silk, College of Textile and Clothing Engineering, Soochow University , Suzhou 215123, China.
authors.forename: Yuekun
authors.initials: Y
authors.lastname: Lai
authors.affiliation: Wenzhou Institute of Biomaterials and Engineering, Chinese Academy of Sciences , 16 Xinsan Road, Wenzhou 325011, China.
authors.forename: Allan Zijian
authors.initials: AZ
authors.lastname: Zhao
authors.affiliation: School of Materials Science and Engineering, Nanyang Technological University , 50 Nanyang Avenue, 639798, Singapore.
authors.forename: Lay Poh
authors.initials: LP
authors.lastname: Tan
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation; D019070:Cell Lineage; D048430:Cell Shape; D002478:Cells, Cultured; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D055616:Microtechnology / Q000379:methods*; D055495:Neurogenesis; D011327:Printing; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D015415:Biomarkers; D016326:Extracellular Matrix Proteins
keywords: biomaterials; electrospinning; microcontact printing; neurogenesis; stem cell