title: Multiple vitellogenins and product yolk proteins in European sea bass (Dicentrarchus labrax): Molecular characterization, quantification in plasma, liver and ovary, and maturational proteolysis.
abstract: Three complete vitellogenin (Vtg) polypeptides of European sea bass (Dicentrarchus labrax), an acanthomorph teleost spawning pelagic eggs in seawater, were deduced from cDNA and identified as VtgAa, VtgAb and VtgC based on current Vtg nomenclature and phylogeny. Label free quantitative mass spectrometry verified the presence of the three sea bass Vtgs or their product yolk proteins (YPs) in liver, plasma and ovary of postvitellogenic females. As evidenced by normalized spectral counts, VtgAb-derived protein was 2- to 5-fold more abundant, depending on sample type, than for VtgAa, while VtgC-derived protein was less abundant, albeit only 3-fold lower than for VtgAb in the ovary. Western blotting with Vtg type-specific antisera raised against corresponding gray mullet (Mugil cephalus) lipovitellins (Lvs) detected all three types of sea bass Vtg in the blood plasma of gravid females and/or estrogenized males and showed that all three forms of sea bass Lv undergo limited partial degradation during oocyte maturation. The comparatively high levels of VtgC-derived YPs in fully-grown oocytes and the maturational proteolysis of all three types of Lv differ from what has been reported for other teleosts spawning pelagic eggs in seawater but are similar to recent findings for two species of North American Moronidae, the striped bass (Morone saxatilis) and white perch (Morone americana), which spawn pelagic and demersal eggs, respectively in fresh water. Together with the high Vtg sequence homologies and virtually identical structural features of each type of Vtg between species, these findings indicate that the moronid multiple Vtg systems do not substantially vary with reproductive environment.
authors.affiliation: Akdeniz University, Fisheries Faculty, Antalya, 07070, Turkey.
authors.forename: Ozlem
authors.initials: O
authors.lastname: Yilmaz
authors.affiliation: Instituto de Ciencias Marinas de Andalucía, Consejo Superior de Investigaciones Científicas, Avda. República Saharaui 2, 11510 Puerto Real, Cádiz, Spain.
authors.forename: Francisco
authors.initials: F
authors.lastname: Prat
authors.affiliation: Instituto de Acuicultura de Torre de la Sal, Consejo Superior de Investigaciones Científicas, Torre de la Sal, s/n 12595, Ribera de Cabanes, Castellòn, Spain.
authors.forename: A Jose
authors.initials: AJ
authors.lastname: Ibáñez
authors.affiliation: Central Research and Immunology Laboratories, Akdeniz University, Faculty of Medicine, Antalya, 07070, Turkey.
authors.forename: Sadi
authors.initials: S
authors.lastname: Köksoy
authors.affiliation: School of Marine Biosciences, Kitasato University, 1-15-1 Kitasato, Minami, Sagamihara, Kanagawa 252-0373, Japan.
authors.forename: Haruna
authors.initials: H
authors.lastname: Amano
authors.affiliation: Department of Biology, College of Agriculture and Life Sciences, North Carolina State University, Raleigh, NC 27695-7617, USA. Electronic address: AquaGyn.NC@gmail.com.
authors.forename: Craig V
authors.initials: CV
authors.lastname: Sullivan
mesh_terms: D000818:Animals; D017756:Aquaculture; D001492:Bass / Q000097:blood / Q000502:physiology*; D004527:Egg Proteins / Q000097:blood / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004958:Estradiol / Q000494:pharmacology; D004967:Estrogens / Q000494:pharmacology; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008522:Mediterranean Sea; D010053:Ovary / Q000187:drug effects / Q000378:metabolism*; D010446:Peptide Fragments / Q000097:blood / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D010802:Phylogeny; D020033:Protein Isoforms / Q000097:blood / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D011499:Protein Processing, Post-Translational / Q000187:drug effects; D021382:Protein Sorting Signals / Q000187:drug effects; D059748:Proteolysis / Q000187:drug effects; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D009626:Terminology as Topic; D014818:Vitellogenesis / Q000187:drug effects; D014819:Vitellogenins / Q000097:blood / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D004527:Egg Proteins; D004967:Estrogens; D010446:Peptide Fragments; D020033:Protein Isoforms; D021382:Protein Sorting Signals; D011994:Recombinant Proteins; D014819:Vitellogenins; D004958:Estradiol; C001549:lipovitellin
keywords: Maturation; Molecular; Moronidae; Oocyte; Ovary; Teleost; Vitellogenin; Yolk