title: Gene analysis of multiple oral bacteria by the polymerase chain reaction coupled with capillary polymer electrophoresis.
abstract: Capillary polymer electrophoresis is identified as a promising technology for the analysis of DNA from bacteria, virus and cell samples. In this paper, we propose an innovative capillary polymer electrophoresis protocol for the quantification of polymerase chain reaction products. The internal standard method was modified and applied to capillary polymer electrophoresis. The precision of our modified internal standard protocol was evaluated by measuring the relative standard deviation of intermediate capillary polymer electrophoresis experiments. Results showed that the relative standard deviation was reduced from 12.4-15.1 to 0.6-2.3%. Linear regression tests were also implemented to validate our protocol. The modified internal standard method showed good linearity and robust properties. Finally, the ease of our method was illustrated by analyzing a real clinical oral sample using a one-run capillary polymer electrophoresis experiment.
authors.affiliation: Engineering Research Center of Optical Instrument and System, University of Shanghai for Science and Technology, Shanghai, China.|Institute of Photonics and Bio-medicine (IPBM), Graduate School of Science, East China University of Science and Technology (ECUST), Shanghai, China.
authors.forename: Chenchen
authors.initials: C
authors.lastname: Liu
authors.affiliation: Institute of Photonics and Bio-medicine (IPBM), Graduate School of Science, East China University of Science and Technology (ECUST), Shanghai, China.|Department of Applied Physics, Graduate School of Engineering, Osaka University, Osaka, Japan.
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Yamaguchi
authors.affiliation: Division of Special Care Dentistry, Osaka University Dental Hospital, Osaka University, Osaka, Japan.|Department of Preventive Dentistry, Osaka University Graduate School of Dentistry Osaka University, Osaka, Japan.
authors.forename: Shinichi
authors.initials: S
authors.lastname: Sekine
authors.affiliation: Institute of Photonics and Bio-medicine (IPBM), Graduate School of Science, East China University of Science and Technology (ECUST), Shanghai, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Ni
authors.affiliation: Engineering Research Center of Optical Instrument and System, University of Shanghai for Science and Technology, Shanghai, China.
authors.forename: Zhenqing
authors.initials: Z
authors.lastname: Li
authors.affiliation: School of Optoelectronic Engineering, ChangZhou Institute of Technology, Changzhou, Jiangsu, China.
authors.forename: Xifang
authors.initials: X
authors.lastname: Zhu
authors.affiliation: Institute of Photonics and Bio-medicine (IPBM), Graduate School of Science, East China University of Science and Technology (ECUST), Shanghai, China.|School of Optoelectronic Engineering, ChangZhou Institute of Technology, Changzhou, Jiangsu, China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Dou
mesh_terms: D001419:Bacteria / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000032:analysis / Q000235:genetics*; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D009055:Mouth / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011108:Polymers / Q000737:chemistry
chemical_list: D001426:Bacterial Proteins; D011108:Polymers
keywords: Capillary polymer electrophoresis; Clinical diagnosis; Internal standard method; Oral bacteria; Quantitative analysis