title: Ideonella paludissp. nov., isolated from a marsh.
abstract: A bacterial strain, designated KBP-31T, was isolated from a water sample taken from the Banping Lake Wetland Park in Taiwan and characterized using a polyphasic taxonomic approach. Cells of strain KBP-31Twere Gram-stain-negative, strictly aerobic, motile, light-yellow rods. Growth occurred at 10-37 °C (optimum 25 °C), at pH 6-8 (optimum pH 6) and with 0-1 % NaCl (w/v, optimum 0 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain KBP-31Tbelonged to the genusIdeonellaand was most closely related toIdeonella dechloratansATCC 51718Twith a sequence similarity of 98.2 %. Strain KBP-31Tcontained summed feature 3 (comprising C16 : 1ω7cand/or C16 : 1ω6c) and C16 : 0as the predominant fatty acids. The major hydroxyl fatty acid was C10 : 03-OH. The polar lipid profile consisted of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, an uncharacterized aminophospholipid and two uncharacterized phospholipids. The DNA G+C content of the genomic DNA was 67.9 mol%. The DNA-DNA relatedness of strain KBP-31Twith respect to recognized species of the genusIdeonellawas less than 70 %. On the basis of the phylogenetic inference and phenotypic data, strain KBP-31Tshould be classified as a novel species, for which the nameIdeonella paludissp. nov. is proposed. The type strain is KBP-31T( = BCRC 80524T= KCTC 32238T).
authors.affiliation: Department of Marine Biotechnology, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd. Nan-Tzu, Kaohsiung City 811, Taiwan.
authors.forename: Shih-Yi
authors.initials: SY
authors.lastname: Sheu
authors.affiliation: Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd. Nan-Tzu, Kaohsiung City 811, Taiwan.
authors.forename: Zih-Han
authors.initials: ZH
authors.lastname: Chen
authors.affiliation: College of Agriculture and Natural Resources, Department of Soil and Environmental Sciences, National Chung Hsing University, Taichung 402, Taiwan.
authors.forename: Chiu-Chung
authors.initials: CC
authors.lastname: Young
authors.affiliation: Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd. Nan-Tzu, Kaohsiung City 811, Taiwan.
authors.forename: Wen-Ming
authors.initials: WM
authors.lastname: Chen