title: Exercise-conditioned plasma attenuates nuclear concentrations of DNA methyltransferase 3B in human peripheral blood mononuclear cells.
abstract: DNA methylation is modifiable by acute and chronic exercise. DNA methyltransferases (DNMT) catalyze this process; however, there is a lack of literature concerning the specific mechanisms by which exercise-induced modifications occur. Interleukin 6 (IL-6) stimulation of various cell lines has been shown to augment DNMT expression and nuclear translocation, which suggests a possible pathway by which exercise is able to elicit changes in epigenetic enzymes. The present study sought to elucidate the response of the de novo methyltransferases DNMT3A and DNMT3B to circulatory factors found in plasma isolated from whole blood before and after 120-min of treadmill running at an intensity of 60% of individual velocity at V˙O2max (vV˙O2max) interspersed with 30-sec sprints at 90% of vV˙O2max every 10-min. Peripheral blood mononuclear cells (PBMCs) isolated from a resting participant were incubated with plasma isolated from exercising participants (n = 10) or recombinant IL-6 (rIL-6), followed by nuclear protein extraction and quantification of DNMT3A and DNMT3B concentrations. Nuclear concentrations of DNMT3B significantly decreased following the experimental protocol (P = 0.03), with no change observed in DNMT3A (P = 0.514).Various concentrations of rIL-6 caused an elevation in both DNMT3A and DNMT3B nuclear concentration compared with the blank control. The conflicting results between exercising and rIL-6 conditions suggests that IL-6 does regulate DNMT nuclear transport, however, other plasma mediators may also exert significant influence on the nuclear concentrations of these enzymes.
authors.affiliation: Faculty of Health and Life Sciences, Northumbria University, Newcastle Upon Tyne, England steven.horsburgh@northumbria.ac.uk.
authors.forename: Steven
authors.initials: S
authors.lastname: Horsburgh
authors.affiliation: Faculty of Health and Life Sciences, Northumbria University, Newcastle Upon Tyne, England.
authors.forename: Stephen
authors.initials: S
authors.lastname: Todryk
authors.affiliation: Research Department, British College of Osteopathic Medicine, London, England.
authors.forename: Christopher
authors.initials: C
authors.lastname: Toms
authors.affiliation: Health and Exercise Sciences Research Group, University of Stirling, Stirling, Scotland.
authors.forename: Colin N
authors.initials: CN
authors.lastname: Moran
authors.affiliation: Faculty of Health and Life Sciences, Northumbria University, Newcastle Upon Tyne, England.
authors.forename: Les
authors.initials: L
authors.lastname: Ansley
keywords: DNA methylation; epigenetics; inflammation