title: Visible to Near-Infrared Fluorescence Enhanced Cellular Imaging on Plasmonic Gold Chips.
abstract: Rapid and sensitive detections of a variety of surface and intracellular proteins, nucleic acids, and other cellular biomarkers are important to elucidating biological signaling pathways and to devising disease diagnostics and therapeutics. Here, sensitive imaging and detection of cellular proteins on fluorescence-enhancing, nanostructured plasmonic gold (pGold) chips is presented. Imaging of fluorescently labeled cellular biomarkers on pGold is enhanced by 2-30-fold in the visible to near infrared (NIR) range of ≈500-900 nm. The high fluorescence enhancement of >700 nm significantly improves the dynamic range and signal/background ratios of NIR imaging, allowing high-performance multicolor imaging in the visible-NIR range using high quantum yield (QY) visible dyes and lower QY NIR fluorophores. Further, multiple cellular proteins of single cells of various cell types can be detected through microarraying of cells, useful for potentially hundreds and thousands different types of cells assayed on a single chip down to small cell numbers. This work suggests a simple, high throughput, high sensitivity, and multiplexed single-cell analysis method on fluorescence enhancing plasmonic substrates in the entire visible to NIR window.
authors.affiliation: Department of Chemistry, Stanford University, Stanford, CA, 94305, USA.
authors.forename: Byumseok
authors.initials: B
authors.lastname: Koh
authors.affiliation: Department of Chemistry, Stanford University, Stanford, CA, 94305, USA.
authors.forename: Xiaoyang
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Chemistry, Stanford University, Stanford, CA, 94305, USA.
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Department of Chemistry, Stanford University, Stanford, CA, 94305, USA.
authors.forename: Bing
authors.initials: B
authors.lastname: Yuan
authors.affiliation: Department of Chemistry, Stanford University, Stanford, CA, 94305, USA.|Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072, P. R. China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Lin
authors.affiliation: Department of Chemistry, Stanford University, Stanford, CA, 94305, USA.
authors.forename: Alexander L
authors.initials: AL
authors.lastname: Antaris
authors.affiliation: Department of Chemistry, Stanford University, Stanford, CA, 94305, USA.
authors.forename: Hao
authors.initials: H
authors.lastname: Wan
authors.affiliation: Department of Chemistry, Stanford University, Stanford, CA, 94305, USA.
authors.forename: Ming
authors.initials: M
authors.lastname: Gong
authors.affiliation: Department of Chemistry, Stanford University, Stanford, CA, 94305, USA.
authors.forename: Jiang
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Chemistry, Stanford University, Stanford, CA, 94305, USA.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Materials Science and Engineering, South University of Science and Technology of China, Shenzhen, 518055, P. R. China.
authors.forename: Yongye
authors.initials: Y
authors.lastname: Liang
authors.affiliation: Department of Chemistry, Stanford University, Stanford, CA, 94305, USA.
authors.forename: Hongjie
authors.initials: H
authors.lastname: Dai
mesh_terms: D045744:Cell Line, Tumor; D006046:Gold / Q000737:chemistry*; D006801:Humans; D007211:Indoles; D007259:Infrared Rays; D008565:Membrane Proteins / Q000378:metabolism; D046228:Microarray Analysis; D008856:Microscopy, Fluorescence; D057054:Molecular Imaging / Q000379:methods*
chemical_list: C427728:IRDye800; D007211:Indoles; D008565:Membrane Proteins; D006046:Gold
keywords: NIR; fluorophores; gold; plasmonics, imaging