title: Nanostructured zirconia decorated reduced graphene oxide based efficient biosensing platform for non-invasive oral cancer detection.
abstract: We report results of the studies relating to fabrication of a non-invasive, label-free and an efficient biosensing platform for detection of the oral cancer biomarker (CYFRA-21-1). One step hydrothermal process was used for uniform decoration of nanostructured zirconia (average particle size 13 nm) on reduced graphene oxide (ZrO2-RGO) to avoid coagulation of the zirconia nanoparticles and to obtain enhanced electrochemical performance of ZrO2-RGO nanocomposite based biosensor. Further, ZrO2-RGO has been functionalized using 3-aminopropyl triethoxy saline (APTES) and electrophoretically deposited on the indium tin oxide coated glass substrate at a low DC potential.The APTES/ZrO2-RGO/ITO electrode exhibits improved heterogeneous electron transfer (more than two times) with respect to that of the APTES/ZrO2/ITO electrode indicating faster electron transfer kinetics. The -NH2 containing APTES/ZrO2-RGO/ITO platform is further biofunctionalized with anti-CYFRA-21-1. The structural and morphological investigations of the ZrO2-RGO based biosensing platform have been accomplished using X-ray diffraction (XRD), electrochemical, transmission electron microscopy (TEM), atomic force microscopy (AFM) and Fourier transform infrared spectroscopy (FT-IR) studies. This immunosensor exhibits a wider linear detection range (2-22 ng mL(-1)), excellent sensitivity (0.756 µA mL ng(-1)) and a remarkable lower detection limit of 0.122 ng mL(-1). The observed results have been validated via enzyme linked immunosorbent assay (ELISA).
authors.affiliation: Nanobioelectronics Laboratory, Department of Biotechnology, Delhi Technological University, Delhi 110042, India.
authors.forename: Suveen
authors.initials: S
authors.lastname: Kumar
authors.affiliation: Nanobioelectronics Laboratory, Department of Biotechnology, Delhi Technological University, Delhi 110042, India.
authors.forename: Jai Gopal
authors.initials: JG
authors.lastname: Sharma
authors.affiliation: Nanobioelectronics Laboratory, Department of Biotechnology, Delhi Technological University, Delhi 110042, India.
authors.forename: Sagar
authors.initials: S
authors.lastname: Maji
authors.affiliation: Nanobioelectronics Laboratory, Department of Biotechnology, Delhi Technological University, Delhi 110042, India. Electronic address: bansi.malhotra@gmail.com.
authors.forename: Bansi Dhar
authors.initials: BD
authors.lastname: Malhotra
mesh_terms: D000951:Antigens, Neoplasm / Q000097:blood* / Q000737:chemistry; D014408:Biomarkers, Tumor / Q000097:blood / Q000302:isolation & purification*; D015374:Biosensing Techniques; D055664:Electrochemical Techniques; D006108:Graphite / Q000737:chemistry; D006801:Humans; D053539:Keratin-19 / Q000097:blood* / Q000737:chemistry; D009062:Mouth Neoplasms / Q000097:blood* / Q000235:genetics; D017550:Spectroscopy, Fourier Transform Infrared; D017971:Tin Compounds / Q000737:chemistry; D014961:X-Ray Diffraction; D015040:Zirconium / Q000737:chemistry
chemical_list: D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D053539:Keratin-19; D017971:Tin Compounds; C102012:antigen CYFRA21.1; C109984:indium tin oxide; D006108:Graphite; D015040:Zirconium; C028541:zirconium oxide
keywords: Biosensor; CYFRA-21-1; Oral cancer; Reduced graphene oxide; Zirconia