title: Type I Interferon Response Limits Astrovirus Replication and Protects against Increased Barrier Permeability In Vitro and In Vivo.
abstract: Little is known about intrinsic epithelial cell responses against astrovirus infection. Here we show that human astrovirus type 1 (HAstV-1) infection induces type I interferon (beta interferon [IFN-β]) production in differentiated Caco2 cells, which not only inhibits viral replication by blocking positive-strand viral RNA and capsid protein synthesis but also protects against HAstV-1-increased barrier permeability. Excitingly, we found similar results in vivo using a murine astrovirus (MuAstV) model, providing new evidence that virus-induced type I IFNs may protect against astrovirus replication and pathogenesis in vivo.
  
  
  OBJECTIVE
  Human astroviruses are a major cause of pediatric diarrhea, yet little is known about the immune response. Here we show that type I interferon limits astrovirus infection and preserves barrier permeability both in vitro and in vivo. Importantly, we characterized a new mouse model for studying astrovirus replication and pathogenesis.
authors.affiliation: Department of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, Tennessee, USA.
authors.forename: Shauna A
authors.initials: SA
authors.lastname: Marvin
authors.affiliation: Department of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, Tennessee, USA Rhodes College, Memphis, Tennessee, USA.
authors.forename: C Theodore
authors.initials: CT
authors.lastname: Huerta
authors.affiliation: Department of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, Tennessee, USA.
authors.forename: Bridgett
authors.initials: B
authors.lastname: Sharp
authors.affiliation: Department of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, Tennessee, USA.
authors.forename: Pamela
authors.initials: P
authors.lastname: Freiden
authors.affiliation: Department of Biological Sciences, California State University, Chico, California, USA tdcline@csuchico.edu stacey.schultz-cherry@stjude.org.
authors.forename: Troy D
authors.initials: TD
authors.lastname: Cline
authors.affiliation: Department of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, Tennessee, USA tdcline@csuchico.edu stacey.schultz-cherry@stjude.org.
authors.forename: Stacey
authors.initials: S
authors.lastname: Schultz-Cherry
mesh_terms: D000818:Animals; D019350:Astroviridae Infections / Q000473:pathology / Q000821:virology; D018938:Caco-2 Cells; D004195:Disease Models, Animal; D004847:Epithelial Cells / Q000276:immunology* / Q000821:virology*; D005260:Female; D006801:Humans; D007370:Interferon Type I / Q000276:immunology*; D008297:Male; D001257:Mamastrovirus / Q000276:immunology* / Q000502:physiology*; D008810:Mice, Inbred C57BL; D010539:Permeability; D014779:Virus Replication
chemical_list: D007370:Interferon Type I