title: In vitro hyperthermia with improved colloidal stability and enhanced SAR of magnetic core/shell nanostructures.
abstract: Magnetic core/shell nanostructures of Fe3O4 nanoparticles coated with oleic acid and betaine-HCl were studied for their possible use in magnetic fluid hyperthermia (MFH). Their colloidal stability and heat induction ability were studied in different media viz. phosphate buffer solution (PBS), saline solution and glucose solution with different physiological conditions and in human serum. The results showed enhanced colloidal stability in these media owing to their high zeta potential values. Heat induction studies showed that specific absorption rates (SAR) of core/shells were 82-94W/g at different pH of PBS and concentrations of NaCl and glucose. Interestingly, core/shells showed 78.45±3.90W/g SAR in human serum. The cytotoxicity of core/shells done on L929 and HeLa cell lines using 3-(4,5-dimethylthiazol-2-yl)2,5-diphenyl tetrazolium bromide and trypan blue dye exclusion assays showed >89% and >80% cell viability for 24 and 48h respectively. Core/shell structures were also found to be very efficient for in vitro MFH on cancer cell line. About 95% cell death was occurred in 90min after hyperthermia treatment. The mechanism of cell death was found to be elevated ROS generation in cells after exposure to core/shells in external magnetic field. This study showed that these core/shells have a great potential to be used in in vivo MFH.
authors.affiliation: Center for Interdisciplinary Research, D.Y.Patil University, Kolhapur 416006, MS, India.
authors.forename: R M
authors.initials: RM
authors.lastname: Patil
authors.affiliation: Center for Interdisciplinary Research, D.Y.Patil University, Kolhapur 416006, MS, India.
authors.forename: N D
authors.initials: ND
authors.lastname: Thorat
authors.affiliation: Center for Interdisciplinary Research, D.Y.Patil University, Kolhapur 416006, MS, India.
authors.forename: P B
authors.initials: PB
authors.lastname: Shete
authors.affiliation: Center for Interdisciplinary Research, D.Y.Patil University, Kolhapur 416006, MS, India; Department of Chemical Engineering, Konkuk University, Hwayang-Dong, Gwangjin-Gu, Seoul 143701, Republic of Korea.
authors.forename: S V
authors.initials: SV
authors.lastname: Otari
authors.affiliation: Center for Interdisciplinary Research, D.Y.Patil University, Kolhapur 416006, MS, India.
authors.forename: B M
authors.initials: BM
authors.lastname: Tiwale
authors.affiliation: Center for Interdisciplinary Research, D.Y.Patil University, Kolhapur 416006, MS, India. Electronic address: pawar_s_h@yahoo.com.
authors.forename: S H
authors.initials: SH
authors.lastname: Pawar
mesh_terms: D000818:Animals; D001622:Betaine / Q000737:chemistry / Q000494:pharmacology; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000494:pharmacology; D003102:Colloids; D006367:HeLa Cells; D006358:Hot Temperature; D006801:Humans; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D008422:Materials Testing; D051379:Mice; D019301:Oleic Acid / Q000737:chemistry / Q000494:pharmacology
chemical_list: D020099:Coated Materials, Biocompatible; D003102:Colloids; D058185:Magnetite Nanoparticles; D019301:Oleic Acid; D001622:Betaine
keywords: Colloidal stability; Cytotoxicity; In vitro hyperthermia; Magnetic fluid hyperthermia; Reactive oxygen species; Specific absorption rate