title: "In-house" production of DNA size marker from a vaccinal Bacillus anthracis strain.
abstract: OBJECTIVE
  DNA molecular weight marker is widely used in molecular biology experiments incurring considerable costs on low-budget settings.
  
  
  METHODS
  Here a PCR-supported procedure is described that uses 10 primer pairs targeting chromosomal DNA from the harmless vaccinal Bacillus anthracis Sterne 34F2 strain as template. A single PCR protocol is used to reproduce all the 10 fragments of a 100 bp DNA size marker.
  
  
  CONCLUSIONS
  The unpurified amalgam of 10 PCR products can be directly loaded to agarose gels. This work was intended to develop a reasonably cost-effective DNA ladder that is useful for researchers in laboratories with limited funding.
authors.affiliation: Department of Veterinary Aerobic Bacteria, Razi Institute, Karaj, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Sekhavati
authors.affiliation: Department of Veterinary Aerobic Bacteria, Razi Institute, Karaj, Iran.
authors.forename: Keyvan
authors.initials: K
authors.lastname: Tadayon
authors.affiliation: Department of Veterinary Aerobic Bacteria, Razi Institute, Karaj, Iran.
authors.forename: Rainak
authors.initials: R
authors.lastname: Ghaderi
authors.affiliation: Department of Veterinary Aerobic Bacteria, Razi Institute, Karaj, Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Banihashemi
authors.affiliation: Department of Veterinary Aerobic Bacteria, Razi Institute, Karaj, Iran.
authors.forename: Ahmad Reza
authors.initials: AR
authors.lastname: Jabbari
authors.affiliation: Department of Veterinary Aerobic Bacteria, Razi Institute, Karaj, Iran.
authors.forename: Gholamreza
authors.initials: G
authors.lastname: Shokri
authors.affiliation: Department of Veterinary Aerobic Bacteria, Razi Institute, Karaj, Iran.
authors.forename: Nasim
authors.initials: N
authors.lastname: Karimnasab
keywords: Bacillus anthracis Sterne 34F2; DNA size marker; PCR; sequencing