title: Regulation of human subcutaneous adipocyte differentiation by EID1.
abstract: Increasing thermogenesis in white adipose tissues can be used to treat individuals at high risk for obesity and cardiovascular disease. The objective of this study was to determine the function of EP300-interacting inhibitor of differentiation (EID1), an inhibitor of muscle differentiation, in the induction of beige adipocytes from adipose mesenchymal stem cells (ADMSCs). Subcutaneous adipose tissue was obtained from healthy women undergoing abdominoplasty. ADMSCs were isolated in vitro, grown, and transfected with EID1 or EID1 siRNA, and differentiation was induced after 48 h by administering rosiglitazone. The effects of EID1 expression under the control of the aP2 promoter (aP2-EID1) were also evaluated in mature adipocytes that were differentiated from ADMSCs. Transfection of EID1 into ADMSCs reduced triglyceride accumulation while increasing levels of thermogenic proteins, such as PGC1α, TFAM, and mitochondrial uncoupling protein 1 (UCP1), all of which are markers of energy expenditure and mitochondrial activity. Furthermore, increased expression of the beige phenotype markers CITED1 and CD137 was observed. Transfection of aP2-EID1 transfection induced the conversion of mature white adipocytes to beige adipocytes, as evidenced by increased expression of PGC1α, UCP1, TFAM, and CITED1. These results indicate that EID1 can modulate ADMSCs, inducing a brown/beige lineage. EID1 may also activate beiging in white adipocytes obtained from subcutaneous human adipose tissue.
authors.affiliation: Center of Biomedical Research (CIBUS)Universidad de La Sabana, Km. 7 Autopista Norte de Bogota, 140013 Chia, ColombiaDepartment of Integrative PhysiologyGunma University, Maebashi, JapanInstitute of Experimental Animal ResearchGunma University, Maebashi, Japan.
authors.forename: Diana
authors.initials: D
authors.lastname: Vargas
authors.affiliation: Center of Biomedical Research (CIBUS)Universidad de La Sabana, Km. 7 Autopista Norte de Bogota, 140013 Chia, ColombiaDepartment of Integrative PhysiologyGunma University, Maebashi, JapanInstitute of Experimental Animal ResearchGunma University, Maebashi, Japan.
authors.forename: Noriaki
authors.initials: N
authors.lastname: Shimokawa
authors.affiliation: Center of Biomedical Research (CIBUS)Universidad de La Sabana, Km. 7 Autopista Norte de Bogota, 140013 Chia, ColombiaDepartment of Integrative PhysiologyGunma University, Maebashi, JapanInstitute of Experimental Animal ResearchGunma University, Maebashi, Japan.
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Kaneko
authors.affiliation: Center of Biomedical Research (CIBUS)Universidad de La Sabana, Km. 7 Autopista Norte de Bogota, 140013 Chia, ColombiaDepartment of Integrative PhysiologyGunma University, Maebashi, JapanInstitute of Experimental Animal ResearchGunma University, Maebashi, Japan.
authors.forename: Wendy
authors.initials: W
authors.lastname: Rosales
authors.affiliation: Center of Biomedical Research (CIBUS)Universidad de La Sabana, Km. 7 Autopista Norte de Bogota, 140013 Chia, ColombiaDepartment of Integrative PhysiologyGunma University, Maebashi, JapanInstitute of Experimental Animal ResearchGunma University, Maebashi, Japan.
authors.forename: Adriana
authors.initials: A
authors.lastname: Parra
authors.affiliation: Center of Biomedical Research (CIBUS)Universidad de La Sabana, Km. 7 Autopista Norte de Bogota, 140013 Chia, ColombiaDepartment of Integrative PhysiologyGunma University, Maebashi, JapanInstitute of Experimental Animal ResearchGunma University, Maebashi, Japan.
authors.forename: Ángela
authors.initials: Á
authors.lastname: Castellanos
authors.affiliation: Center of Biomedical Research (CIBUS)Universidad de La Sabana, Km. 7 Autopista Norte de Bogota, 140013 Chia, ColombiaDepartment of Integrative PhysiologyGunma University, Maebashi, JapanInstitute of Experimental Animal ResearchGunma University, Maebashi, Japan.
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Koibuchi
authors.affiliation: Center of Biomedical Research (CIBUS)Universidad de La Sabana, Km. 7 Autopista Norte de Bogota, 140013 Chia, ColombiaDepartment of Integrative PhysiologyGunma University, Maebashi, JapanInstitute of Experimental Animal ResearchGunma University, Maebashi, Japan fernando.lizcano@unisabana.edu.co.
authors.forename: Fernando
authors.initials: F
authors.lastname: Lizcano
mesh_terms: D052438:Adipocytes, White / Q000502:physiology*; D050156:Adipogenesis; D000328:Adult; D018797:Cell Cycle Proteins; D002478:Cells, Cultured; D005260:Female; D015870:Gene Expression; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D047495:PPAR gamma / Q000502:physiology; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D050151:Subcutaneous Fat / Q000166:cytology; D055815:Young Adult
chemical_list: D018797:Cell Cycle Proteins; C416925:EID1 protein, human; D009687:Nuclear Proteins; D047495:PPAR gamma; D012097:Repressor Proteins
keywords: ADMSCs; EID1; PPARg; beige fat; retinoblastoma