title: Inhibition of TLR8 mediated signaling promotes BCG induced apoptosis in THP-1 cells.
abstract: Apoptosis was considered as one of the important host defense mechanisms against mycobacteria infection. In macrophage, the main target cell of Mycobacterium tuberculosis, apoptosis after infection could help kill the bacillus inside and process the antigens for further presentation and proper immune response. Here, we identified a role of TLR8 during the apoptosis induced by Bacillus Calmette Guérin (BCG) infection in THP-1 cells. Knockdown TLR8 further increased the apoptosis induced by BCG infection, and this enhanced apoptosis was caspase-dependent. During this process, Erk1/2, JNK and NFκB pathways were negatively affected and contributed to the enhanced apoptosis.
authors.affiliation: Institute of Laboratory Animal Sciences, Peking Union Medical College, Beijing, China; Key Laboratory of Human Disease Comparative Medicine, Ministry of Health, Beijing, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Tang
authors.affiliation: Institute of Laboratory Animal Sciences, Peking Union Medical College, Beijing, China; Key Laboratory of Human Disease Comparative Medicine, Ministry of Health, Beijing, China. Electronic address: Zhanlj@cnilas.org.
authors.forename: Lingjun
authors.initials: L
authors.lastname: Zhan
authors.affiliation: Institute of Laboratory Animal Sciences, Peking Union Medical College, Beijing, China; Key Laboratory of Human Disease Comparative Medicine, Ministry of Health, Beijing, China. Electronic address: chuanqin@vip.sina.com.
authors.forename: Chuan
authors.initials: C
authors.lastname: Qin
mesh_terms: D000818:Animals; D017209:Apoptosis; D002417:Cattle; D002460:Cell Line; D008264:Macrophages / Q000166:cytology* / Q000378:metabolism / Q000382:microbiology; D009163:Mycobacterium bovis / Q000235:genetics / Q000502:physiology*; D015398:Signal Transduction; D051194:Toll-Like Receptor 1 / Q000235:genetics / Q000378:metabolism*; D014380:Tuberculosis, Bovine / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology / Q000503:physiopathology*
chemical_list: D051194:Toll-Like Receptor 1
keywords: Apoptosis; Bacillus Calmette Guérin; Mycobacterium; TLR8