title: Detection and preliminary screening of the human gene expression profile for Hirschsprung's disease.
abstract: The present study investigated a genome microarray of colorectal lesions (spasm segments) in children with Hirschsprung's disease (HSCR), and analyzed the results. In addition, the present study screened for differentially expressed genes in children with HSCR. Microarray technology was used to examine the human gene expression profiles of the colorectal lesions (spasm segments) of six children with HSCR, and three normal colon tissue samples. The data were analyzed be determining P‑values of significance and absolute fold changes. Preliminary screening was performed to identify genes exhibiting significant differential expression in children with HSCR, and these target genes were analyzed in subsequent verification and analytical investigations. Of >20,000 detected human genes, the preliminary screenings demonstrated that 3,850 genes were differentially expressed and upregulated, with P<0.05 and >2‑fold absolute changes in expression. In addition, 645 differentially expressed genes with P<0.05 and >2‑fold absolute changes were downregulated. Of the upregulated genes, 118 were involved in classic signaling pathways, compared with 11 of the downregulated genes (P<0.001; absolute fold change >2‑fold). HSCR etiology is complex and often involves multiple gene changes. Microarray technology can produce large quantities of gene expression data simultaneously, and analyzing this data using various techniques may provide a fast and efficient method for identifying novel gene targets and for investigating the mechanisms underlying HSCR pathogenesis.
authors.affiliation: Tumour Laboratory of Children's Hospital of Chongqing Medical University, Chongqing 400014, P.R. China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.affiliation: Tumour Laboratory of Children's Hospital of Chongqing Medical University, Chongqing 400014, P.R. China.
authors.forename: Shiqi
authors.initials: S
authors.lastname: Wang
authors.affiliation: Tumour Laboratory of Children's Hospital of Chongqing Medical University, Chongqing 400014, P.R. China.
authors.forename: Xianqing
authors.initials: X
authors.lastname: Jin
authors.affiliation: Tumour Laboratory of Children's Hospital of Chongqing Medical University, Chongqing 400014, P.R. China.
authors.forename: Ning
authors.initials: N
authors.lastname: Wang
authors.affiliation: Tumour Laboratory of Children's Hospital of Chongqing Medical University, Chongqing 400014, P.R. China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Luo
authors.affiliation: Tumour Laboratory of Children's Hospital of Chongqing Medical University, Chongqing 400014, P.R. China.
authors.forename: Yinping
authors.initials: Y
authors.lastname: Teng
mesh_terms: D002675:Child, Preschool; D015536:Down-Regulation / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling; D063990:Gene Ontology; D006627:Hirschsprung Disease / Q000235:genetics*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D012313:RNA / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D015854:Up-Regulation / Q000235:genetics
chemical_list: D012313:RNA