title: Cell cycle-dependent changes in H3K56ac in human cells.
abstract: The incorporation of histone H3 with an acetylated lysine 56 (H3K56ac) into the nucleosome is important for chromatin remodeling and serves as a marker of new nucleosomes during DNA replication and repair in yeast. However, in human cells, the level of H3K56ac is greatly reduced, and its role during the cell cycle is controversial. Our aim was to determine the potential of H3K56ac to regulate cell cycle progression in different human cell lines. A significant increase in the number of H3K56ac foci, but not in H3K56ac protein levels, was observed during the S and G2 phases in cancer cell lines, but was not observed in embryonic stem cell lines. Despite this increase, the H3K56ac signal was not present in late replication chromatin, and H3K56ac protein levels did not decrease after the inhibition of DNA replication. H3K56ac was not tightly associated with the chromatin and was primarily localized to active chromatin regions. Our results support the role of H3K56ac in transcriptionally active chromatin areas but do not confirm H3K56ac as a marker of newly synthetized nucleosomes in DNA replication.
authors.affiliation: a Centre for Biomedical Image Analysis; Faculty of Informatics; Masaryk University ; Brno , Czech Republic.
authors.forename: Stanislav
authors.initials: S
authors.lastname: Stejskal
authors.affiliation: a Centre for Biomedical Image Analysis; Faculty of Informatics; Masaryk University ; Brno , Czech Republic.
authors.forename: Karel
authors.initials: K
authors.lastname: Stepka
authors.affiliation: a Centre for Biomedical Image Analysis; Faculty of Informatics; Masaryk University ; Brno , Czech Republic.
authors.forename: Lenka
authors.initials: L
authors.lastname: Tesarova
authors.affiliation: b Research Group - Proteomics; Central European Institute of Technology; Masaryk University ; Brno , Czech Republic.|c National Centre for Biomolecular Research; Faculty of Science; Masaryk University ; Brno , Czech Republic.
authors.forename: Karel
authors.initials: K
authors.lastname: Stejskal
authors.affiliation: a Centre for Biomedical Image Analysis; Faculty of Informatics; Masaryk University ; Brno , Czech Republic.
authors.forename: Martina
authors.initials: M
authors.lastname: Matejkova
authors.affiliation: a Centre for Biomedical Image Analysis; Faculty of Informatics; Masaryk University ; Brno , Czech Republic.
authors.forename: Pavel
authors.initials: P
authors.lastname: Simara
authors.affiliation: b Research Group - Proteomics; Central European Institute of Technology; Masaryk University ; Brno , Czech Republic.|c National Centre for Biomolecular Research; Faculty of Science; Masaryk University ; Brno , Czech Republic.
authors.forename: Zbynek
authors.initials: Z
authors.lastname: Zdrahal
authors.affiliation: a Centre for Biomedical Image Analysis; Faculty of Informatics; Masaryk University ; Brno , Czech Republic.
authors.forename: Irena
authors.initials: I
authors.lastname: Koutna
mesh_terms: D002453:Cell Cycle / Q000235:genetics / Q000502:physiology*; D002843:Chromatin / Q000378:metabolism*; D004261:DNA Replication / Q000235:genetics / Q000502:physiology; D016195:G2 Phase / Q000235:genetics; D018922:HL-60 Cells; D006657:Histones / Q000378:metabolism*; D006801:Humans; D013058:Mass Spectrometry; D009707:Nucleosomes / Q000378:metabolism; D016196:S Phase / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D002843:Chromatin; D006657:Histones; D009707:Nucleosomes; D029701:Saccharomyces cerevisiae Proteins
keywords: Cell cycle; Chromatin; DNA replication; H3K56ac; Mammalian cells; Nucleosome