title: Cloning, expression, and antiviral activity of interferon β from the Chinese microbat, Myotis davidii.
abstract: Bats are natural reservoir hosts for many viruses that produce no clinical symptoms in bats. Therefore, bats may have evolved effective mechanisms to control viral replication. However, little information is available on bat immune responses to viral infection. Type I interferon (IFN) plays a key role in controlling viral infections. In this study, we report the cloning, expression, and biological activity of interferon β (IFNβ) from the Chinese microbat species, Myotis davidii. We demonstrated the upregulation of IFNB and IFN-stimulated genes in a kidney cell line derived from M. davidii after treatment with polyI:C or infection with Sendai virus. Furthermore, the recombinant IFNβ inhibited vesicular stomatitis virus and bat adenovirus replication in cell lines from two bat species, M. davidii and Rhinolophus sinicus. We provide the first in vitro evidence of IFNβ antiviral activity in microbats, which has important implications for virus interactions with these hosts.
authors.affiliation: Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China.
authors.forename: Ying-Zi
authors.initials: YZ
authors.lastname: Liang
authors.affiliation: Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China.
authors.forename: Li-Jun
authors.initials: LJ
authors.lastname: Wu
authors.affiliation: Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Program in Emerging Infectious Diseases, Duke-National University of Singapore Graduate Medical School, Singapore, 169857, Singapore.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China.
authors.forename: Mei-Niang
authors.initials: MN
authors.lastname: Wang
authors.affiliation: Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China.
authors.forename: Xing-Lou
authors.initials: XL
authors.lastname: Yang
authors.affiliation: Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China.
authors.forename: Xing-Yi
authors.initials: XY
authors.lastname: Ge
authors.affiliation: Program in Emerging Infectious Diseases, Duke-National University of Singapore Graduate Medical School, Singapore, 169857, Singapore.
authors.forename: Lin-Fa
authors.initials: LF
authors.lastname: Wang
authors.affiliation: Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China. zlshi@wh.iov.cn.
authors.forename: Zheng-Li
authors.initials: ZL
authors.lastname: Shi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D002460:Cell Line; D002685:Chiroptera / Q000235:genetics* / Q000276:immunology* / Q000821:virology; D003001:Cloning, Molecular; D006801:Humans; D007113:Immunity, Innate; D007370:Interferon Type I / Q000494:pharmacology; D016899:Interferon-beta / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology* / Q000494:pharmacology; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein; D017385:Sequence Homology; D015854:Up-Regulation; D018116:Vesiculovirus / Q000187:drug effects / Q000502:physiology
chemical_list: D000998:Antiviral Agents; D007370:Interferon Type I; D016899:Interferon-beta
keywords: IFN-stimulated genes; antiviral activity; bat; interferon