title: High-Frequency Acceleration: Therapeutic Tool to Preserve Bone following Tooth Extractions.
abstract: A common problem in clinical dentistry is the significant and rapid bone loss that occurs after tooth extraction. Currently there is no solution for the long-term preservation of alveolar bone. Previously, we showed that high-frequency acceleration (HFA) has an osteogenic effect on healthy alveolar bone. However, it is not known if HFA can preserve alveolar bone after extraction without negatively affecting wound healing. The purpose of this study was to evaluate the effect of HFA on alveolar bone loss and the rate of bone formation after tooth extraction. Eighty-five adult Sprague-Dawley rats were divided into 3 groups: control, static (static load), and HFA. In all groups, the maxillary right third molar was extracted. The HFA group received HFA for 5 min/d, applied through the second molar. The static group received the same magnitude of static load. The control group did not receive any stimulation. Some animals received fluorescent dyes at 26 and 54 d. Samples were collected on days 0, 7, 14, 28, and 56 for fluorescence microscopy, micro-computed tomography, histology, RNA, and protein analyses. We found that HFA increased bone volume in the extraction site and surrounding alveolar bone by 44% when compared with static, while fully preserving alveolar bone height and width long-term. These effects were accompanied by increased expression of osteogenic markers and intramembranous bone formation and by decreased expression of osteoclastic markers and bone resorption activity, as well as decreased expression of many inflammatory markers. HFA is a noninvasive safe treatment that can be used to prevent alveolar bone loss and/or accelerate bone healing after tooth extraction.
authors.affiliation: Consortium for Translational Orthodontic Research, New York University College of Dentistry, New York, NY, USA Department of Orthodontics, New York University College of Dentistry, New York, NY, USA Department of Developmental Biology, Harvard School of Dental Medicine, Boston, MA, USA Department of Oral and Maxillofacial Surgery, New York University College of Dentistry, New York, NY, USA.
authors.forename: M
authors.initials: M
authors.lastname: Alikhani
authors.affiliation: Consortium for Translational Orthodontic Research, New York University College of Dentistry, New York, NY, USA Department of Orthodontics, New York University College of Dentistry, New York, NY, USA.
authors.forename: J A
authors.initials: JA
authors.lastname: Lopez
authors.affiliation: Consortium for Translational Orthodontic Research, New York University College of Dentistry, New York, NY, USA.
authors.forename: H
authors.initials: H
authors.lastname: Alabdullah
authors.affiliation: Consortium for Translational Orthodontic Research, New York University College of Dentistry, New York, NY, USA.
authors.forename: T
authors.initials: T
authors.lastname: Vongthongleur
authors.affiliation: Consortium for Translational Orthodontic Research, New York University College of Dentistry, New York, NY, USA.
authors.forename: C
authors.initials: C
authors.lastname: Sangsuwon
authors.affiliation: Consortium for Translational Orthodontic Research, New York University College of Dentistry, New York, NY, USA.
authors.forename: M
authors.initials: M
authors.lastname: Alikhani
authors.affiliation: Consortium for Translational Orthodontic Research, New York University College of Dentistry, New York, NY, USA Department of Orthodontics, New York University College of Dentistry, New York, NY, USA.
authors.forename: S
authors.initials: S
authors.lastname: Alansari
authors.affiliation: Consortium for Translational Orthodontic Research, New York University College of Dentistry, New York, NY, USA Department of Mechanical Engineering, Institute Polytechnic of Viseu, Viseu, Portugal.
authors.forename: S M
authors.initials: SM
authors.lastname: Oliveira
authors.affiliation: Consortium for Translational Orthodontic Research, New York University College of Dentistry, New York, NY, USA Department of Orthodontics, New York University College of Dentistry, New York, NY, USA.
authors.forename: J M
authors.initials: JM
authors.lastname: Nervina
authors.affiliation: Consortium for Translational Orthodontic Research, New York University College of Dentistry, New York, NY, USA Department of Orthodontics, New York University College of Dentistry, New York, NY, USA Department of Basic Science and Craniofacial Biology, New York University College of Dentistry, New York, NY, USA cristina.teixeira@nyu.edu.
authors.forename: C C
authors.initials: CC
authors.lastname: Teixeira
mesh_terms: D000054:Acceleration; D016301:Alveolar Bone Loss / Q000517:prevention & control*; D000539:Alveolar Process / Q000502:physiology; D000818:Animals; D001696:Biomechanical Phenomena; D001862:Bone Resorption / Q000517:prevention & control; D005456:Fluorescent Dyes; D008297:Male; D008437:Maxilla / Q000502:physiology; D008856:Microscopy, Fluorescence; D008964:Molar, Third / Q000601:surgery; D010010:Osteoclasts / Q000502:physiology; D010012:Osteogenesis / Q000502:physiology*; D010812:Physical Stimulation; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D014081:Tooth Extraction / Q000379:methods*; D020390:Tooth Socket / Q000502:physiology*; D014732:Vibration / Q000627:therapeutic use*; D014945:Wound Healing / Q000502:physiology; D055114:X-Ray Microtomography / Q000379:methods
chemical_list: D005456:Fluorescent Dyes
keywords: alveolar bone; bone formation; mechanical stimulation; osteogenic response; socket healing; vibration