title: Antioxidant effects of vitamin C on hemoglobin-based oxygen carriers derived from human cord blood.
abstract: OBJECTIVE
  To investigated the antioxidant protection effects of vitamin C (Vc) on the hemoglobin-based oxygen carriers (HBOCs) solution.
  
  
  METHODS
  Three influential variables were studied including the molar ratio of Vc and hemoglobin, pH and temperature. The HBOCs molecules were characterized through spectral analysis, circular dichroism spectra, the oxygen binding curves and the molecular weights.
  
  
  RESULTS
  The optimized conditions were molar ratios of above 3, pH of 7.5-8.0 and temperature of 4 °C. HBOCs characterization showed no differences before and after Vc addition.
  
  
  CONCLUSIONS
  Antioxidant effects in HBOCs solution could be presented by Vc, indicating the potential application of Vc in HBOCs development.
authors.affiliation: a Institute of Blood Transfusion, Chinese Academy of Medical Science , Chengdu , P. R. China ;
authors.forename: Gang
authors.initials: G
authors.lastname: Chen
authors.affiliation: a Institute of Blood Transfusion, Chinese Academy of Medical Science , Chengdu , P. R. China ;
authors.forename: Yingying
authors.initials: Y
authors.lastname: Duan
authors.affiliation: a Institute of Blood Transfusion, Chinese Academy of Medical Science , Chengdu , P. R. China ;
authors.forename: Jiaxin
authors.initials: J
authors.lastname: Liu
authors.affiliation: a Institute of Blood Transfusion, Chinese Academy of Medical Science , Chengdu , P. R. China ;
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.affiliation: a Institute of Blood Transfusion, Chinese Academy of Medical Science , Chengdu , P. R. China ;|b Institute of Tianjin Union Biological Science Development , Tianjin , P. R. China.
authors.forename: Chengmin
authors.initials: C
authors.lastname: Yang
mesh_terms: D000975:Antioxidants / Q000737:chemistry*; D001205:Ascorbic Acid / Q000737:chemistry*; D001692:Biological Transport; D001802:Blood Substitutes / Q000737:chemistry*; D005312:Fetal Blood / Q000737:chemistry*; D005333:Fetus; D006454:Hemoglobins / Q000737:chemistry* / Q000302:isolation & purification; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D010100:Oxygen / Q000737:chemistry*; D013696:Temperature
chemical_list: D000975:Antioxidants; D001802:Blood Substitutes; D006454:Hemoglobins; D001205:Ascorbic Acid; D010100:Oxygen
keywords: antioxidant effects; hemoglobin-based oxygen carriers; methemoglobin; vitamin C