title: Electrochemical immunosensor for interferon-γ based on disposable ITO detector and HRP-antibody-conjugated nano gold as signal tag.
abstract: Tuberculosis is the most frequent cause of infection-related death worldwide. A new disposable electrochemical immunosensor with low cost and simple fabrication was proposed to detect interferon-γ (IFN-γ). Diallyldimethylammonium chloride (PDDA) and Au nanoparticle (AuNP) composite were used to provide an efficient biointerface, horseradish peroxidase (HRP)-labeled antibody-conjugated AuNP (HRP-Ab2-AuNP) bioconjugates were used as a novel signal tag. The large amounts of HRP on the signal tag can catalyze the oxidation of Hydroquinone (HQ) by H2O2, which can induce an amplified reductive current. The catalytic reduction current was related to the amount of HRP immobilized on the surface, which itself was related to the concentration of IFN-γ. Under optimized conditions, the proposed immunosensor showed a high sensitivity and a linear range of 0.1-10,000pg/mL with a detection limit of 0.048pg/mL. The assay results of clinical serum samples obtained by the immunosensor were in acceptable agreement with the reference values. Therefore, the immunosensor possessed excellent clinical value in early diagnosis and control of tuberculosis.
authors.affiliation: Department of Chemistry, Jinan University, Guangzhou 510632, People's Republic of China.
authors.forename: Yaru
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Chemistry, Jinan University, Guangzhou 510632, People's Republic of China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Department of Chemistry, Jinan University, Guangzhou 510632, People's Republic of China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Ye
authors.affiliation: Department of Microbiology and Immunology, Jinan University, Guangzhou 510632, People's Republic of China; Guangdong Key Laboratory of Molecular Immunology and Antibody Engineering, Jinan University, Guangzhou 510632, People's Republic of China.
authors.forename: Yuqi
authors.initials: Y
authors.lastname: Yan
authors.affiliation: Department of Chemistry, Jinan University, Guangzhou 510632, People's Republic of China.
authors.forename: Langhuan
authors.initials: L
authors.lastname: Huang
authors.affiliation: Department of Microbiology and Immunology, Jinan University, Guangzhou 510632, People's Republic of China; Guangdong Key Laboratory of Molecular Immunology and Antibody Engineering, Jinan University, Guangzhou 510632, People's Republic of China.
authors.forename: Zhenyou
authors.initials: Z
authors.lastname: Jiang
authors.affiliation: Department of Chemistry, Jinan University, Guangzhou 510632, People's Republic of China. Electronic address: tanshaozao@163.com.
authors.forename: Shaozao
authors.initials: S
authors.lastname: Tan
authors.affiliation: Department of Light Chemical Engineering, Guangdong Polytechnic, Foshan 528041, People's Republic of China. Electronic address: cecaixiang@163.com.
authors.forename: Xiang
authors.initials: X
authors.lastname: Cai
mesh_terms: D055664:Electrochemical Techniques / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D006046:Gold / Q000737:chemistry* / Q000378:metabolism; D006735:Horseradish Peroxidase / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods; D007371:Interferon-gamma / Q000032:analysis* / Q000097:blood; D058726:Nanoconjugates; D017971:Tin Compounds / Q000737:chemistry*
chemical_list: D058726:Nanoconjugates; D017971:Tin Compounds; C091521:horseradish peroxidase-coupled gold; C109984:indium tin oxide; D006046:Gold; D007371:Interferon-gamma; D006735:Horseradish Peroxidase
keywords: Au nanoparticles; Disposable electrode; Interferon-gamma