title: [Improving isoprene production by engineered heterologous mevalonate pathway in Escherichia coli].
abstract: Isoprene is an important precursor of synthetic rubber material. In our previous study, metabolic engineered Escherichia coli strain (BW-01) was constructed and used to produce isoprene. Based on the theory of protein budget, using synthetic biology strategies including the increased copy number of genes and rare codons, we regulated the expression of key enzyme to improve isoprene production in Escherichia coli strain. Under shake-flask conditions, isoprene productivity of the engineered strain (BW-07) increased by 73% compared with BW-01, reached 761.1 mg/L. It provides a reference for further studies.
authors.forename: Fan
authors.initials: F
authors.lastname: Feng
authors.forename: Yang
authors.initials: Y
authors.lastname: Xu
authors.forename: Yong
authors.initials: Y
authors.lastname: Tao
authors.forename: Weifeng
authors.initials: W
authors.lastname: Liu
authors.forename: Baixue
authors.initials: B
authors.lastname: Lin
mesh_terms: D002070:Butadienes; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D018628:Gene Dosage; D045782:Hemiterpenes / Q000096:biosynthesis*; D007218:Industrial Microbiology; D060847:Metabolic Engineering; D008798:Mevalonic Acid; D010420:Pentanes; D058615:Synthetic Biology
chemical_list: D002070:Butadienes; D045782:Hemiterpenes; D010420:Pentanes; C005059:isoprene; D008798:Mevalonic Acid