title: Selection of the Strain Lactobacillus acidophilus ATCC 43121 and Its Application to Brewers' Spent Grain Conversion into Lactic Acid.
abstract: Six Lactobacillus strains were analyzed to select a bacterium for conversion of brewers' spent grain (BSG) into lactic acid. Among the investigated strains, L. acidophilus ATCC 43121 showed the highest yield of lactic acid production (16.1 g/L after 48 hours) when grown in a synthetic medium. It was then analyzed for its ability to grow on the hydrolysates obtained from BSG after acid-alkaline (AAT) or aqueous ammonia soaking (AAS) pretreatment. The lactic acid production by L. acidophilus ATCC 43121 through fermentation of the hydrolysate from AAS treated BSG was 96% higher than that from the AAT treated one, although similar yields of lactic acid per consumed glucose were achieved due to a higher (46%) glucose consumption by L. acidophilus ATCC 43121 in the AAS BSG hydrolysate. It is worth noting that adding yeast extract to the BSG hydrolysates increased both the yield of lactic acid per substrate consumed and the volumetric productivity. The best results were obtained by fermentation of AAS BSG hydrolysate supplemented by yeast extract, in which the strain produced 22.16 g/L of lactic acid (yield of 0.61 g/g), 27% higher than the value (17.49 g/L) obtained in the absence of a nitrogen source.
authors.affiliation: Department of Chemical Sciences, University of Naples "Federico II", Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126 Naples, Italy.
authors.forename: Rossana
authors.initials: R
authors.lastname: Liguori
authors.affiliation: Department of Bioprocess Engineering and Biotechnology, Federal University of Paraná, Coronel Francisco H. dos Santos Avenue 210, 81531-990 Curitiba, PR, Brazil.
authors.forename: Carlos Ricardo
authors.initials: CR
authors.lastname: Soccol
authors.affiliation: Department of Bioprocess Engineering and Biotechnology, Federal University of Paraná, Coronel Francisco H. dos Santos Avenue 210, 81531-990 Curitiba, PR, Brazil.
authors.forename: Luciana Porto de Souza
authors.initials: LP
authors.lastname: Vandenberghe
authors.affiliation: Department of Bioprocess Engineering and Biotechnology, Federal University of Paraná, Coronel Francisco H. dos Santos Avenue 210, 81531-990 Curitiba, PR, Brazil.
authors.forename: Adenise Lorenci
authors.initials: AL
authors.lastname: Woiciechowski
authors.affiliation: Institute of Agro-Environmental and Forest Biology (IBAF), UOS Napoli, National Council of Research, Via Pietro Castellino 111, 80131 Naples, Italy.
authors.forename: Elena
authors.initials: E
authors.lastname: Ionata
authors.affiliation: Institute of Agro-Environmental and Forest Biology (IBAF), UOS Napoli, National Council of Research, Via Pietro Castellino 111, 80131 Naples, Italy.
authors.forename: Loredana
authors.initials: L
authors.lastname: Marcolongo
authors.affiliation: Department of Chemical Sciences, University of Naples "Federico II", Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126 Naples, Italy.
authors.forename: Vincenza
authors.initials: V
authors.lastname: Faraco
mesh_terms: D000641:Ammonia / Q000737:chemistry; D003470:Culture Media; D002523:Edible Grain / Q000737:chemistry / Q000378:metabolism*; D005285:Fermentation; D005947:Glucose / Q000737:chemistry; D019344:Lactic Acid / Q000096:biosynthesis* / Q000737:chemistry; D007779:Lactobacillus acidophilus / Q000737:chemistry / Q000378:metabolism*; D009584:Nitrogen / Q000737:chemistry
chemical_list: D003470:Culture Media; D019344:Lactic Acid; D000641:Ammonia; D005947:Glucose; D009584:Nitrogen