title: Molecular cloning and characterization of lymphocyte cell kinase from humphead snapper (Lutjanus sanguineus).
abstract: Lymphocyte cell kinase (LCK) belongs to the Src family of tyrosine kinases, which involves in the proliferation control of lymphocytes. In this study, we cloned the LCK gene of humphead snapper (Lutjanus sanguineus) (designed as LsLCK). Sequence analysis showed that the full-length cDNA of LsLCK was 2279 bp, contained a 1506-bp open reading frame (ORF), encoding a polypeptide of 501 amino acids. The deduced amino acid possessed the typical structural features of known LCK proteins, including four Src homology (SH) domains arranged as the SH1 domain followed by a regulatory C-terminal tail (COOH-domain), SH2 and SH3 adapter domains and SH4 domain which required for membrane attachment and CD4/CD8 binding. Fluorescent quantitative real-time PCR analysis indicated that LsLCK transcripts were expressed mainly in thymus, spleen and head kidney in healthy fish. Moreover, the mRNA expressions in these tissues were significantly up-regulated after challenge with Vibrio harveyi. The results of immunohistochemistry showed that LsLCK protein localized distinctly in cytoplasm of cell in thymus, spleen and head kidney. Taken together, these findings indicated that LsLCK may play an important role in the immune response of humphead snapper against bacterial infection.
authors.affiliation: College of Fishery, Guangdong Ocean University, Zhanjiang, China.|Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Zhanjiang, China.|Guangdong Key Laboratory of Control for Diseases of Aquatic Economic Animals, Zhanjiang, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Huang
authors.affiliation: College of Fishery, Guangdong Ocean University, Zhanjiang, China.|Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Zhanjiang, China.|Guangdong Key Laboratory of Control for Diseases of Aquatic Economic Animals, Zhanjiang, China.
authors.forename: J
authors.initials: J
authors.lastname: Cai
authors.affiliation: College of Fishery, Guangdong Ocean University, Zhanjiang, China.|Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Zhanjiang, China.|Guangdong Key Laboratory of Control for Diseases of Aquatic Economic Animals, Zhanjiang, China.
authors.forename: B
authors.initials: B
authors.lastname: Wang
authors.affiliation: College of Fishery, Guangdong Ocean University, Zhanjiang, China.|Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Zhanjiang, China.|Guangdong Key Laboratory of Control for Diseases of Aquatic Economic Animals, Zhanjiang, China.
authors.forename: J-F
authors.initials: JF
authors.lastname: Tang
authors.affiliation: College of Fishery, Guangdong Ocean University, Zhanjiang, China.|Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Zhanjiang, China.|Guangdong Key Laboratory of Control for Diseases of Aquatic Economic Animals, Zhanjiang, China.
authors.forename: J-C
authors.initials: JC
authors.lastname: Jian
authors.affiliation: Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Zhanjiang, China.|Guangdong Key Laboratory of Control for Diseases of Aquatic Economic Animals, Zhanjiang, China.
authors.forename: Z-H
authors.initials: ZH
authors.lastname: Wu
authors.affiliation: College of Fishery, Guangdong Ocean University, Zhanjiang, China.|Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Zhanjiang, China.|Guangdong Key Laboratory of Control for Diseases of Aquatic Economic Animals, Zhanjiang, China.
authors.forename: Z
authors.initials: Z
authors.lastname: Gan
authors.affiliation: College of Fishery, Guangdong Ocean University, Zhanjiang, China.|Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Zhanjiang, China.|Guangdong Key Laboratory of Control for Diseases of Aquatic Economic Animals, Zhanjiang, China.
authors.forename: Y-S
authors.initials: YS
authors.lastname: Lu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D005393:Fish Diseases / Q000201:enzymology / Q000276:immunology; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation / Q000276:immunology; D007113:Immunity, Innate / Q000235:genetics; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck) / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D010473:Perciformes / Q000145:classification / Q000235:genetics* / Q000276:immunology*; D010802:Phylogeny; D016415:Sequence Alignment / Q000662:veterinary; D014733:Vibrio / Q000502:physiology; D014735:Vibrio Infections / Q000201:enzymology / Q000276:immunology / Q000662:veterinary
chemical_list: D018076:DNA, Complementary; D029941:Fish Proteins; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck)
keywords: Lutjanus sanguineus; Vibrio harveyi; immunohistochemistry; lymphocyte cell kinase