title: In vitro Penetration and in vivo Distribution of Honokiol into the Intervertebral Disc in Rat.
abstract: Honokiol is a potential candidate for the treatment of intervertebral disc (IVD) degeneration. In this study, we develop in vitro and in vivo methods to detect the distribution of honokiol in intervertebral discs using high-performance liquid chromatography. A rat tail disc was used for both experimental models. For the in vivo animal experiment, blood samples and tail discs were collected at 15, 30, 60, 120 and 240 min after honokiol administration (30 mg/kg, i.v.). The analyte was separated by a mobile phase of methanol and 10 mM NaH2PO4 buffer at pH 2.8 (78:22, v/v) and pumped through a reversed-phase analytical column (250 × 4.6 mm, particle size 5 μm) at room temperature. The in vitro experimental results demonstrated that honokiol diffused into the intervertebral disc and was concentration-dependent. The active concentration is obtained for the therapeutic level at 15 and 30 min after honokiol administration in the in vivo model.
authors.affiliation: Institute of Traditional Medicine, National Yang Ming University.
authors.forename: Chia-Hsien
authors.initials: CH
authors.lastname: Chen
authors.forename: Chang-Jung
authors.initials: CJ
authors.lastname: Chiang
authors.forename: Lien-Chen
authors.initials: LC
authors.lastname: Wu
authors.forename: Chih-Hong
authors.initials: CH
authors.lastname: Yang
authors.forename: Yi-Jie
authors.initials: YJ
authors.lastname: Kuo
authors.forename: Tung-Hu
authors.initials: TH
authors.lastname: Tsai
mesh_terms: D000098:Acetophenones / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics*; D001713:Biphenyl Compounds / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics*; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000493:pharmacokinetics*; D066298:In Vitro Techniques; D007275:Injections, Intravenous; D007403:Intervertebral Disc / Q000378:metabolism*; D017705:Lignans / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics*; D008297:Male; D015394:Molecular Structure; D010539:Permeability; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D014018:Tissue Distribution; D000498:Allyl Compounds; D010636:Phenols
chemical_list: D000098:Acetophenones; D000893:Anti-Inflammatory Agents; D001713:Biphenyl Compounds; D004365:Drugs, Chinese Herbal; D017705:Lignans; C005499:honokiol; D000498:Allyl Compounds; D010636:Phenols; C013638:paeonol