title: PbWoxT1 mRNA from pear (Pyrus betulaefolia) undergoes long-distance transport assisted by a polypyrimidine tract binding protein.
abstract: Little is known about the mechanisms by which mRNAs are transported over long distances in the phloem between the rootstock and the scion in grafted woody plants. We identified an mRNA in the pear variety 'Du Li' (Pyrus betulaefolia) that was shown to be transportable in the phloem. It contains a WUSCHEL-RELATED HOMEOBOX (WOX) domain and was therefore named Wox Transport 1 (PbWoxT1). A 548-bp fragment of PbWoxT1 is critical in long-distance transport. PbWoxT1 is rich in CUCU polypyrimidine domains and its mRNAs interact with a polypyrimidine tract binding protein, PbPTB3. Furthermore, the expression of PbWoxT1 significantly increased in the stems of wild-type (WT) tobacco grafted onto the rootstocks of PbWoxT1 or PbPTB3 co-overexpressing lines, but this was not the case in WT plants grafted onto PbWoxT1 overexpressing rootstocks, suggesting that PbPTB3 mediates PbWoxT1 mRNA long-distance transport. We provide novel information that adds a new mechanism with which to explain the noncell-autonomous manner of WOX gene function, which enriches our understanding of how WOX genes work in fruit trees and other species.
authors.affiliation: Laboratory of Fruit Cell and Molecular Breeding, China Agricultural University, Beijing, 100193, China.
authors.forename: Xuwei
authors.initials: X
authors.lastname: Duan
authors.affiliation: Laboratory of Fruit Cell and Molecular Breeding, China Agricultural University, Beijing, 100193, China.
authors.forename: Wenna
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Laboratory of Fruit Cell and Molecular Breeding, China Agricultural University, Beijing, 100193, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Huang
authors.affiliation: Laboratory of Fruit Cell and Molecular Breeding, China Agricultural University, Beijing, 100193, China.
authors.forename: Li
authors.initials: L
authors.lastname: Hao
authors.affiliation: Laboratory of Fruit Cell and Molecular Breeding, China Agricultural University, Beijing, 100193, China.
authors.forename: Shengnan
authors.initials: S
authors.lastname: Wang
authors.affiliation: College of Horticulture, Shenyang Agricultural University, Shenyang, 110866, China.
authors.forename: Aide
authors.initials: A
authors.lastname: Wang
authors.affiliation: Laboratory of Fruit Cell and Molecular Breeding, China Agricultural University, Beijing, 100193, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Meng
authors.affiliation: Laboratory of Fruit Cell and Molecular Breeding, China Agricultural University, Beijing, 100193, China.
authors.forename: Qiulei
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Laboratory of Fruit Cell and Molecular Breeding, China Agricultural University, Beijing, 100193, China.
authors.forename: Qiuju
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Laboratory of Fruit Cell and Molecular Breeding, China Agricultural University, Beijing, 100193, China.
authors.forename: Tianzhong
authors.initials: T
authors.lastname: Li
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D016000:Cluster Analysis; D018506:Gene Expression Regulation, Plant; D052585:Phloem / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D038941:Polypyrimidine Tract-Binding Protein / Q000235:genetics* / Q000378:metabolism; D011485:Protein Binding; D034443:RNA Transport / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014026:Nicotiana / Q000235:genetics
chemical_list: D010940:Plant Proteins; D012333:RNA, Messenger; D038941:Polypyrimidine Tract-Binding Protein
keywords: PbPTB3; PbWoxT1; Pyrus betulaefolia; mRNA transport; phloem; ribonucleoprotein (RNP) complex