title: Immunoregulation of Bone Marrow-Derived Mesenchymal Stem Cells on the Chronic Cigarette Smoking-Induced Lung Inflammation in Rats.
abstract: Impact of bone mesenchymal stem cell (BMSC) transfusion on chronic smoking-induced lung inflammation is poorly understood. In this study, a rat model of smoking-related lung injury was induced and the rats were treated with vehicle or BMSCs for two weeks. Different subsets of CD4+ T cells, cytokines, and anti-elastin in the lungs as well as the lung injury were characterized. Serum and lung inducible nitric oxide synthase (iNOS) and STAT5 phosphorylation in lymphocytes from lung tissue were also analyzed. Results indicated that transfusion of BMSCs significantly reduced the chronic smoking-induced lung injury, inflammation, and levels of lung anti-elastin in rats. The frequency of Th1 and Th17 cells and the levels of IL-2, IL-6, IFN-γ, TNF-α, IL-17, IP-10, and MCP-1 increased, but the frequency of Tregs and IL-10 decreased. Transfusion of BMSCs significantly modulated the imbalance of immune responses by mitigating chronic smoking-increased Th1 and Th17 responses, but enhancing Treg responses in the lungs of rats. Transfusion of BMSCs limited chronic smoking-related reduction in the levels of serum and lung iNOS and mitigated smoking-induced STAT5 phosphorylation in lymphocytes from lung tissue. BMSCs negatively regulated smoking-induced autoimmune responses in the lungs of rats and may be promising for the intervention of chronic smoking-related lung injury.
authors.affiliation: The First Clinical Medical College, Shanxi Medical University, Taiyuan 030001, China ; Department of Respiratory Medicine, Shanxi DAYI Hospital of Shanxi Medical University, Taiyuan 030032, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Li
authors.affiliation: The First Clinical Medical College, Shanxi Medical University, Taiyuan 030001, China.
authors.forename: Junyan
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Respiratory Medicine, Shanxi DAYI Hospital of Shanxi Medical University, Taiyuan 030032, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Cao
authors.affiliation: The First Clinical Medical College, Shanxi Medical University, Taiyuan 030001, China.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Ma
authors.affiliation: The First Clinical Medical College, Shanxi Medical University, Taiyuan 030001, China ; Department of Respiratory Medicine, Shanxi DAYI Hospital of Shanxi Medical University, Taiyuan 030032, China.
authors.forename: Jianying
authors.initials: J
authors.lastname: Xu
mesh_terms: D000818:Animals; D016207:Cytokines / Q000378:metabolism; D008168:Lung / Q000166:cytology / Q000276:immunology; D008297:Male; D059630:Mesenchymal Stem Cells / Q000276:immunology*; D011014:Pneumonia / Q000276:immunology* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012907:Smoking / Q000276:immunology*
chemical_list: D016207:Cytokines