title: Genome sequence of the clover symbiont Rhizobium leguminosarum bv. trifolii strain CC275e.
abstract: Rhizobium leguminosarum bv. trifolii strain CC275e is a highly effective, N2-fixing microsymbiont of white clover (Trifolium repens L.). The bacterium has been widely used in both Australia and New Zealand as a clover seed inoculant and, as such, has delivered the equivalent of millions of dollars of nitrogen into these pastoral systems. R. leguminosarum strain CC275e is a rod-shaped, motile, Gram-negative, non-spore forming bacterium. The genome was sequenced on an Illumina MiSeq instrument using a 2 × 150 bp paired end library and assembled into 29 scaffolds. The genome size is 7,077,367 nucleotides, with a GC content of 60.9 %. The final, high-quality draft genome contains 6693 protein coding genes, close to 85 % of which were assigned to COG categories. This Whole Genome Shotgun project has been deposited at DDBJ/EMBL/GenBank under the accession JRXL00000000. The sequencing of this genome will enable identification of genetic traits associated with host compatibility and high N2 fixation characteristics in Rhizobium leguminosarum. The sequence will also be useful for development of strain-specific markers to assess factors associated with environmental fitness, competiveness for host nodule occupancy, and survival on legume seeds (New Zealand Ministry of Business, Innovation and Employment program, 'Improving forage legume-rhizobia performance' contract C10X1308 and DairyNZ Ltd.).
authors.affiliation: University of Bordeaux, IT Science, 351 Cours de la Libération, 33400 Talence, France.
authors.forename: Clément
authors.initials: C
authors.lastname: Delestre
authors.affiliation: AgResearch Ltd, Lincoln Campus, Private Bag 4749, Christchurch, 8140 New Zealand.
authors.forename: Aurélie
authors.initials: A
authors.lastname: Laugraud
authors.affiliation: Faculty of Agriculture and Life Sciences, Lincoln University, PO Box 84, Christchurch, New Zealand.
authors.forename: Hayley
authors.initials: H
authors.lastname: Ridgway
authors.affiliation: Department of Microbiology and Immunology, University of Otago, PO Box 56, Dunedin, New Zealand.
authors.forename: Clive
authors.initials: C
authors.lastname: Ronson
authors.affiliation: AgResearch Ltd, Lincoln Campus, Private Bag 4749, Christchurch, 8140 New Zealand.
authors.forename: Maureen
authors.initials: M
authors.lastname: O'Callaghan
authors.affiliation: AgResearch Ltd, Grasslands Research Centre, Private Bag 11008, Palmerston North, New Zealand.
authors.forename: Brent
authors.initials: B
authors.lastname: Barrett
authors.affiliation: South Australian Research and Development Institute, Urrbrae, South Australia Australia.
authors.forename: Ross
authors.initials: R
authors.lastname: Ballard
authors.affiliation: AgResearch Ltd, Grasslands Research Centre, Private Bag 11008, Palmerston North, New Zealand.
authors.forename: Andrew
authors.initials: A
authors.lastname: Griffiths
authors.affiliation: AgResearch Ltd, Lincoln Campus, Private Bag 4749, Christchurch, 8140 New Zealand.
authors.forename: Sandra
authors.initials: S
authors.lastname: Young
authors.affiliation: Faculty of Agriculture and Life Sciences, Lincoln University, PO Box 84, Christchurch, New Zealand.
authors.forename: Celine
authors.initials: C
authors.lastname: Blond
authors.affiliation: AgResearch Ltd, Lincoln Campus, Private Bag 4749, Christchurch, 8140 New Zealand.
authors.forename: Emily
authors.initials: E
authors.lastname: Gerard
authors.affiliation: AgResearch Ltd, Lincoln Campus, Private Bag 4749, Christchurch, 8140 New Zealand.
authors.forename: Steve
authors.initials: S
authors.lastname: Wakelin
keywords: Alphaproteobacteria; Microsymbiont; Nitrogen fixation; Rhizobia; Root-nodule bacteria