title: [Changes in lung injury and oxidative stress of Sprague-Dawley rats after single intratracheal instillation of potassium dichromate].
abstract: OBJECTIVE
  To investigate the changes in lung injury and oxidative stress of sprague-Dawleyy (SD) rats at different times after single intratracheal instillation of potassium dichromate.
  
  
  METHODS
  A total of 50 healthy male SD rats were randomly divided into control group and potassium dichromate group. The potassium dichromate group and the control group received 3 ml/kg intratracheal instillation of K2Cr2O7 (1.5 mg/kg) and normal saline, respectively. Rats in these two groups were sacrificed in batches at 1, 3, 7, 14, and 28 days after exposure. The changes in the following indices were observed and analyzed: body weight, lung coefficient, alkaline phosphatase (AKP) in bronchoalveolar lavage fluid, glutathione peroxidase (GSH-Px) in lung homogenate, and reduced glutathione (GSH) in serum.
  
  
  RESULTS
  The rats in the potassium dichromate group had significantly decreased body weight on day 1 and day 3 after exposure than the control group (P<0.05). Lung coefficient increased significantly on day 7 (P<0.05) and kept increasing until the end of the experiment. The potassium dichromate group had a significantly higher activity of AKP than the control group on day 1 and day 7 after exposure (P<0.05). However, the potassium dichromate group had a significantly lower activity of GSH-Px than the control group on day 1 and day 3 after exposure (P<0.05). And the potassium dichromate group had a lower activity of reduced GSH than the control group on day 3 and day 7 after exposure.
  
  
  CONCLUSIONS
  Single intratracheal instillation of 1.5 mg/kg potassium dichromate could result in lung inflammatory injury. of SD rats, and the injury is more severe on day 7 after exposure. Body injury is related to antioxidant activity, and the antioxidant.activity cannot recover completely on day 28 after exposure.
authors.forename: Tianjing
authors.initials: T
authors.lastname: Wang
authors.forename: Yanshuang
authors.initials: Y
authors.lastname: Song
authors.forename: Guang
authors.initials: G
authors.lastname: Jia
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid; D004195:Disease Models, Animal; D005978:Glutathione / Q000097:blood; D005979:Glutathione Peroxidase / Q000378:metabolism; D008168:Lung / Q000503:physiopathology; D055370:Lung Injury / Q000139:chemically induced / Q000503:physiopathology*; D008297:Male; D018384:Oxidative Stress; D011192:Potassium Dichromate / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D005979:Glutathione Peroxidase; D000469:Alkaline Phosphatase; D005978:Glutathione; D011192:Potassium Dichromate