title: Myo19 is an outer mitochondrial membrane motor and effector of starvation-induced filopodia.
abstract: Mitochondria respond to environmental cues and stress conditions. Additionally, the disruption of the mitochondrial network dynamics and its distribution is implicated in a variety of neurodegenerative diseases. Here, we reveal a new function for Myo19 in mitochondrial dynamics and localization during the cellular response to glucose starvation. Ectopically expressed Myo19 localized with mitochondria to the tips of starvation-induced filopodia. Corollary to this, RNA interference (RNAi)-mediated knockdown of Myo19 diminished filopodia formation without evident effects on the mitochondrial network. We analyzed the Myo19-mitochondria interaction, and demonstrated that Myo19 is uniquely anchored to the outer mitochondrial membrane (OMM) through a 30-45-residue motif, indicating that Myo19 is a stably attached OMM molecular motor. Our work reveals a new function for Myo19 in mitochondrial positioning under stress.
authors.affiliation: Department of Biology, Technion Israel Institute of Technology, Haifa 3200003, Israel.
authors.forename: Boris I
authors.initials: BI
authors.lastname: Shneyer
authors.affiliation: Department of Biology, Technion Israel Institute of Technology, Haifa 3200003, Israel.
authors.forename: Marko
authors.initials: M
authors.lastname: Ušaj
authors.affiliation: Department of Biology, Technion Israel Institute of Technology, Haifa 3200003, Israel arnon.henn@technion.ac.il.
authors.forename: Arnon
authors.initials: A
authors.lastname: Henn
mesh_terms: D002460:Cell Line; D057809:HEK293 Cells; D006801:Humans; D008928:Mitochondria / Q000378:metabolism*; D063154:Mitochondrial Dynamics / Q000502:physiology*; D051336:Mitochondrial Membranes / Q000378:metabolism*; D020409:Molecular Motor Proteins / Q000378:metabolism*; D009218:Myosins / Q000378:metabolism*; D011554:Pseudopodia / Q000378:metabolism*; D034622:RNA Interference / Q000502:physiology; D013217:Starvation / Q000378:metabolism*
chemical_list: D020409:Molecular Motor Proteins; C548150:Myo19 protein, human; D009218:Myosins
keywords: Filopodia; Mitochondria; Myosin 19