title: Novel two-step method to form silk fibroin fibrous hydrogel.
abstract: Hydrogels prepared by silk fibroin solution have been studied. However, mimicking the nanofibrous structures of extracellular matrix for fabricating biomaterials remains a challenge. Here, a novel two-step method was applied to prepare fibrous hydrogels using regenerated silk fibroin solution containing nanofibrils in a range of tens to hundreds of nanometers. When the gelation process of silk solution occurred, it showed a top-down type gel within 30min. After gelation, silk fibroin fibrous hydrogels exhibited nanofiber network morphology with β-sheet structure. Moreover, the compressive stress and modulus of fibrous hydrogels were 31.9±2.6 and 2.8±0.8kPa, respectively, which was formed using 2.0wt.% concentration solutions. In addition, fibrous hydrogels supported BMSCs attachment and proliferation over 12days. This study provides important insight in the in vitro processing of silk fibroin into useful new materials.
authors.affiliation: The College of Textiles & Fashion, Qingdao University, Qingdao 266071, People's Republic of China; National Engineering Laboratory for Modern Silk, Soochow University, Suzhou 215123, People's Republic of China. Electronic address: mingjinfa@qdu.edu.cn.
authors.forename: Jinfa
authors.initials: J
authors.lastname: Ming
authors.affiliation: The College of Textiles & Fashion, Qingdao University, Qingdao 266071, People's Republic of China.
authors.forename: Mengmeng
authors.initials: M
authors.lastname: Li
authors.affiliation: The College of Textiles & Fashion, Qingdao University, Qingdao 266071, People's Republic of China.
authors.forename: Yuhui
authors.initials: Y
authors.lastname: Han
authors.affiliation: The College of Textiles & Fashion, Qingdao University, Qingdao 266071, People's Republic of China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.affiliation: The College of Textiles & Fashion, Qingdao University, Qingdao 266071, People's Republic of China.
authors.forename: Han
authors.initials: H
authors.lastname: Li
authors.affiliation: National Engineering Laboratory for Modern Silk, Soochow University, Suzhou 215123, People's Republic of China. Electronic address: bqzuo@suda.edu.cn.
authors.forename: Baoqi
authors.initials: B
authors.lastname: Zuo
authors.affiliation: The College of Textiles & Fashion, Qingdao University, Qingdao 266071, People's Republic of China. Electronic address: pfkui@163.com.
authors.forename: Fukui
authors.initials: F
authors.lastname: Pan
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism*; D005349:Fibroins / Q000145:classification*; D020100:Hydrogels / Q000737:chemistry*; D008297:Male; D008422:Materials Testing; D057139:Nanofibers / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D020100:Hydrogels; D005349:Fibroins
keywords: Fibrous; Hydrogel; Silk fibroin