title: Heat shock proteins 27 and 70 contribute to the protection of Schisandrin B against d-galactosamine-induced liver injury in mice.
abstract: Schisandrin B is a hepatoprotective component isolated from a traditional Chinese herb, Schisandra chinensis (Turcz.) Baill. This study determined the effect of Schisandrin B on d-galactosamine -induced liver injury and the role of heat shock proteins 27 and 70 against liver injury in mice. Acute liver injury was induced by intraperitoneal injection of d-galactosamine to mice, and Schisandrin B was given orally. The protein and gene expression of heat shock proteins 27 and 70 were detected by western blot and real-time quantitative polymerase chain reaction, respectively. Liver tissues were subjected to histological evaluation, and the activities of alanine aminotransferase and aspartate aminotransferase in the serum were measured. Pretreatment of Schisandrin B significantly attenuated d-galactosamine-induced liver injury in mice. This result was evidenced by improved alteration of histopathological hepatic necrosis and reduced alanine aminotransferase and aspartate aminotransferase activities in the serum. The hepatoprotective effect was accompanied with overexpression of heat shock proteins 27 and 70 both at the protein and mRNA levels. However, the aforementioned actions of Schisandrin B were all markedly suppressed by the heat shock protein inhibitor quercetin. Heat shock proteins 27 and 70 were involved in the protective effect of Schisandrin B against d-galactosamine-induced liver injury in mice.
authors.affiliation: a Polygenic Disease Institute, Qiqihar Medical University, 333 Bukui Street, Jianhua District, Qiqihar 161006, P. R. China.
authors.forename: Zhiying
authors.initials: Z
authors.lastname: Gao
authors.affiliation: b Department of Gastroenterology and Hepatology, Beijing Chaoyang Hospital, Capital Medical University, 5 Jingyuan Road, Shijingshan District, Beijing 100043, P. R. China.
authors.forename: Jishun
authors.initials: J
authors.lastname: Zhang
authors.affiliation: c Department of Pharmacology, Qiqihar Medical University, 333 Bukui Street, Jianhua District, Qiqihar 161006, P. R. China.
authors.forename: Libo
authors.initials: L
authors.lastname: Li
authors.affiliation: d School of Basic Medicine, Qiqihar Medical University, 333 Bukui Street, Jianhua District, Qiqihar 161006, P. R. China.
authors.forename: Longqing
authors.initials: L
authors.lastname: Shen
authors.affiliation: c Department of Pharmacology, Qiqihar Medical University, 333 Bukui Street, Jianhua District, Qiqihar 161006, P. R. China.
authors.forename: Qingyi
authors.initials: Q
authors.lastname: Li
authors.affiliation: c Department of Pharmacology, Qiqihar Medical University, 333 Bukui Street, Jianhua District, Qiqihar 161006, P. R. China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zou
authors.affiliation: c Department of Pharmacology, Qiqihar Medical University, 333 Bukui Street, Jianhua District, Qiqihar 161006, P. R. China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Du
authors.affiliation: d School of Basic Medicine, Qiqihar Medical University, 333 Bukui Street, Jianhua District, Qiqihar 161006, P. R. China.
authors.forename: Zhibo
authors.initials: Z
authors.lastname: Zhao
mesh_terms: D000410:Alanine Transaminase / Q000378:metabolism; D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy* / Q000209:etiology / Q000378:metabolism; D034242:Cyclooctanes / Q000494:pharmacology; D005688:Galactosamine / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects; D055551:HSP27 Heat-Shock Proteins / Q000378:metabolism*; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D017705:Lignans / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D011083:Polycyclic Compounds / Q000494:pharmacology*; D020011:Protective Agents / Q000494:pharmacology*; D011794:Quercetin / Q000494:pharmacology; D032141:Schisandra / Q000737:chemistry
chemical_list: D034242:Cyclooctanes; D055551:HSP27 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; C486699:Hspb2 protein, mouse; D017705:Lignans; D011083:Polycyclic Compounds; D020011:Protective Agents; C015499:schizandrin B; D005688:Galactosamine; D011794:Quercetin; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase
keywords: Quercetin; Schisandrin B; d-galactosamine; heat shock proteins; induction; liver injury; lésion hépatique; mice; protection; protéines de choc thermique; quercétine; schisandrine B; souris