title: CD45RO+ memory T-cells produce IL-17 in patients with atherosclerosis.
abstract: Several CD4+ T helper (Th) cell subsets are shown to play a role in atherosclerotic lesion formation and progression. We investigated the frequencies of IL-17 and IFN-γ producing CD4+ T-cell subsets in the peripheral blood mononuclear cells (PBMCs) of 10 patients with atherosclerosis and 6 individuals with normal/insignificant coronary artery disease. Th1 and Th17 memory and effector T-cells were enumerated by flowcytometry and correlated with the clinical data and lipid profiles of the subjects. We found the ex-vivo (P=0.0001) and in-vitro production of IL-17 (P=0.001) but not IFN-γ by CD4+ memory T-cells of patients. CD45RO+ memory cells were the major producers of IL-17 and the CD4+CD45RO+PD-1- T-cells of the patients produced higher levels of IFN-γ than controls (P=0.02). Positive correlations between the frequency of CD4+CD45RO+IL-17+IFN-γ- T-cells and serum LDL-C (P=0.007), triglyceride (P=0.02), and systolic (P=0.001) and diastolic (P=0.009) blood pressures (BP) were found. The frequency of CD4+CD45RO+IL-17-IFN-γ- T-cells, which was higher in controls than patients, showed negative correlations with the serum LDL-C (P=0.01) and triglyceride (P=0.02) levels and systolic (P=0.003) and diastolic (P=0.01) BPs. The ex-vivo Th17 deviation of memory T-cells in atherosclerosis and high PD-1 expression are associated with the correlates of atherogenesis such as LDL, TG, and BP.
authors.affiliation: Shiraz University of Medical Sciences Department of Immunology, School of Medicine Shiraz Iran.
authors.forename: N
authors.initials: N
authors.lastname: Behnamfar
authors.affiliation: Shiraz University of Medical Sciences Cardiovascular Research Center Shiraz Iran.
authors.forename: M J
authors.initials: MJ
authors.lastname: Zibaeenezhad
authors.affiliation: Shiraz University of Medical Sciences Department of Immunology, School of Medicine Shiraz Iran.
authors.forename: H
authors.initials: H
authors.lastname: Golmoghaddam
authors.affiliation: Shiraz University of Medical Sciences Department of Immunology, School of Medicine Shiraz Iran mdoroud@sums.ac.ir.
authors.forename: M
authors.initials: M
authors.lastname: Doroudchi
mesh_terms: D000328:Adult; D050197:Atherosclerosis / Q000097:blood / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D001794:Blood Pressure; D015704:CD4 Antigens / Q000235:genetics / Q000276:immunology; D016022:Case-Control Studies; D008078:Cholesterol, LDL / Q000097:blood*; D003971:Diastole; D005260:Female; D005434:Flow Cytometry; D005786:Gene Expression Regulation; D006801:Humans; D007156:Immunologic Memory / Q000235:genetics*; D016130:Immunophenotyping; D007371:Interferon-gamma / Q000235:genetics / Q000276:immunology; D020381:Interleukin-17 / Q000235:genetics / Q000276:immunology*; D017493:Leukocyte Common Antigens / Q000235:genetics / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D061026:Programmed Cell Death 1 Receptor / Q000235:genetics / Q000276:immunology; D015398:Signal Transduction; D013599:Systole; D018417:Th1 Cells / Q000276:immunology / Q000473:pathology; D058504:Th17 Cells / Q000276:immunology* / Q000473:pathology; D014280:Triglycerides / Q000097:blood
chemical_list: D015704:CD4 Antigens; D008078:Cholesterol, LDL; D020381:Interleukin-17; C105992:PDCD1 protein, human; D061026:Programmed Cell Death 1 Receptor; D014280:Triglycerides; D007371:Interferon-gamma; D017493:Leukocyte Common Antigens