title: Quantitation of the Oral Anticoagulants Dabigatran, Rivaroxaban, Apixaban, and Warfarin in Plasma Using Ultra-Performance Liquid Chromatography with Tandem Mass Spectrometry (UPLC-MS/MS).
abstract: This chapter describes a method to measure the oral anticoagulants dabigatran, rivaroxaban, apixaban, and warfarin in plasma samples using ultra-performance liquid chromatography combined with tandem mass spectrometry (UPLC-MS/MS). The instrument is operated in multiple reaction monitoring (MRM) mode with an electrospray ionization (ESI) source in positive ionization mode. Samples are extracted with a 90:10 methanol/0.1 N hydrochloric acid solution containing stable isotope-labeled internal standards for each analyte. After centrifugation the supernatant is transferred to a mass spectrometry vial, injected onto the UPLC-ESI-MS/MS, and quantified using an eight-point calibration curve.
authors.affiliation: Department of Pathology, University Hospitals Case Medical Center, 11100 Euclid Avenue, PTH 5077, Cleveland, OH, 44106, USA. jaime.noguez@uhhospitals.org.
authors.forename: Jaime H
authors.initials: JH
authors.lastname: Noguez
authors.affiliation: Department of Pathology and Laboratory Medicine, Emory University Hospital, Atlanta, GA, USA.
authors.forename: James C
authors.initials: JC
authors.lastname: Ritchie
mesh_terms: D000925:Anticoagulants / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D000069604:Dabigatran / Q000097:blood*; D016903:Drug Monitoring / Q000379:methods; D006801:Humans; D011720:Pyrazoles / Q000097:blood*; D011728:Pyridones / Q000097:blood*; D000069552:Rivaroxaban / Q000097:blood*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014859:Warfarin / Q000097:blood*
chemical_list: D000925:Anticoagulants; D011720:Pyrazoles; D011728:Pyridones; C522181:apixaban; D014859:Warfarin; D000069552:Rivaroxaban; D000069604:Dabigatran
keywords: Anticoagulant; Apixaban; Dabigatran; Mass spectrometry; Rivaroxaban; Warfarin