title: Effects of nano bamboo charcoal on PAHs-degrading strain Sphingomonas sp. GY2B.
abstract: Nano bamboo charcoal (NBC) has been commonly used in the production of textiles, plastics, paint, etc. However, little is known regarding their effects towards the microorganisms. The effects of NBC on phenanthrene degrading strain Sphingomonas sp. GY2B were investigated in the present study. Results showed that the addition of NBC could improve the phenanthrene removal by Sphingomonas sp. GY2B, with removal efficiencies increased by 10.29-18.56% in comparison to the control at 24h, and phenanthrene was almost completely removed at 48h. With the presence of low dose of NBC (20 and 50mgL(-1)), strain GY2B displayed a better growth at 6h, suggesting that NBC was beneficial to the growth of GY2B and thus resulting in the quick removal of phenanthrene from water. However, the growth of strain GY2B in high dose of NBC (200mgL(-1)) was inhibited at 6h, and the inhibition could be attenuated and eliminated after 12h. NBC-effected phenanthrene solubility experiment suggested that NBC makes a negligible contribution to the solubilization of phenanthrene in water. Results of electronic microscopy analysis (SEM and TEM) indicated NBC may interact with the cell membrane, causing the enhanced membrane permeability and then NBC adsorbed on the membrane would enter into the cells. The findings of this work would provide important information for the future usage and long-term environmental risk assessment of NBC.
authors.affiliation: School of Environment and Energy, South China University of Technology, Guangzhou 510006, China.
authors.forename: Bojia
authors.initials: B
authors.lastname: She
authors.affiliation: School of Environmental Science and Engineering, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China.
authors.forename: Xueqin
authors.initials: X
authors.lastname: Tao
authors.affiliation: School of Environment and Energy, South China University of Technology, Guangzhou 510006, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Huang
authors.affiliation: School of Environment and Energy, South China University of Technology, Guangzhou 510006, China; Guangdong Provincial Engineering and Technology Research Center for Environmental Risk Prevention and Emergency Disposal, South China University of Technology, Guangzhou 510006, China; The Key Laboratory of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education, South China University of Technology, Guangzhou 510006, China. Electronic address: GNLu@foxmail.com.
authors.forename: Guining
authors.initials: G
authors.lastname: Lu
authors.affiliation: School of Environment and Energy, South China University of Technology, Guangzhou 510006, China.
authors.forename: Zhili
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: School of Environment and Energy, South China University of Technology, Guangzhou 510006, China; The Key Laboratory of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education, South China University of Technology, Guangzhou 510006, China.
authors.forename: Chuling
authors.initials: C
authors.lastname: Guo
authors.affiliation: School of Environment and Energy, South China University of Technology, Guangzhou 510006, China; The Key Laboratory of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education, South China University of Technology, Guangzhou 510006, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Dang
mesh_terms: D000327:Adsorption / Q000187:drug effects; D001673:Biodegradation, Environmental; D002462:Cell Membrane / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002606:Charcoal / Q000378:metabolism / Q000494:pharmacology*; D053758:Nanoparticles / Q000378:metabolism; D010616:Phenanthrenes / Q000378:metabolism*; D012995:Solubility / Q000187:drug effects; D020578:Sphingomonas / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism
chemical_list: D010616:Phenanthrenes; D002606:Charcoal; C031181:phenanthrene
keywords: Biodegradation; Membrane permeability; Nano bamboo charcoal (NBC); Phenanthrene; Sphingomonas sp. GY2B