title: Enhanced peroxidase-like activity of porphyrin functionalized ceria nanorods for sensitive and selective colorimetric detection of glucose.
abstract: Ceria nanorods modified with 5,10,15,20-tetrakis(4-carboxyl phenyl)-porphyrin (H2TCPP) were prepared. These nanocomposites (H2TCPP-CeO2) exhibited the intrinsic peroxidase-like activity and could catalyze the oxidation of classical peroxidase substrate 3,3',5,5'-tetramethylbiphenyl dihydrochloride (TMB·2HCl) in the presence of H2O2 to produce a typical color reaction from colorless to blue. Our results demonstrated that both the H2TCPP-CeO2 nanocomposites and CeO2 nanorods exhibited higher thermal durance than that of HRP. The affinity of The H2TCPP-CeO2 nanocomposites toward H2O2 and TMB is similar to that of HRP. Fluorescent results indicated that the catalytic mechanism of the H2TCPP-CeO2 nanocomposites were from the decomposition of H2O2 into hydroxyl radicals. Based on these studies, a simple, sensitive, and selective visual and colorimetric method using TMB as the substrate was designed to detect glucose when combined with glucose oxidase. The proposed colorimetric method can detect H2O2 at a low detection limit of 6.1×10(-6)M and a dynamic range of 10(-5)-10(-4)mol·L(-1). This method can also detect glucose at a low detection limit of 3.3×10(-5)mol·L(-1) and a dynamic range of 5.0×10(-5)-1.0×10(-4)mol·L(-1).
authors.affiliation: School of Chemistry and Environmental Engineering, Shandong University of Science and Technology, Qingdao 266510, PR China. Electronic address: qyliu@sdust.edu.cn.
authors.forename: Qingyun
authors.initials: Q
authors.lastname: Liu
authors.affiliation: School of Chemistry and Environmental Engineering, Shandong University of Science and Technology, Qingdao 266510, PR China.
authors.forename: Yanyuan
authors.initials: Y
authors.lastname: Ding
authors.affiliation: School of Chemistry and Environmental Engineering, Shandong University of Science and Technology, Qingdao 266510, PR China.
authors.forename: Yanting
authors.initials: Y
authors.lastname: Yang
authors.affiliation: School of Chemistry and Environmental Engineering, Shandong University of Science and Technology, Qingdao 266510, PR China.
authors.forename: Leyou
authors.initials: L
authors.lastname: Zhang
authors.affiliation: School of Chemistry and Environmental Engineering, Shandong University of Science and Technology, Qingdao 266510, PR China.
authors.forename: Lifang
authors.initials: L
authors.lastname: Sun
authors.affiliation: School of Chemistry and Environmental Engineering, Shandong University of Science and Technology, Qingdao 266510, PR China.
authors.forename: Pengpeng
authors.initials: P
authors.lastname: Chen
authors.affiliation: School of Chemistry and Environmental Engineering, Shandong University of Science and Technology, Qingdao 266510, PR China.
authors.forename: Chun
authors.initials: C
authors.lastname: Gao
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D002563:Cerium / Q000737:chemistry*; D003124:Colorimetry / Q000379:methods; D005453:Fluorescence; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000737:chemistry*; D043942:Nanotubes / Q000737:chemistry*; D011166:Porphyrins / Q000737:chemistry*
chemical_list: D011166:Porphyrins; D002563:Cerium; C030583:ceric oxide; D005949:Glucose Oxidase; D005947:Glucose
keywords: CeO(2) nanorods; Colorimetric; Nanocomposites; Peroxidase; Porphyrin