title: Protein 4.1G Regulates Cell Adhesion, Spreading, and Migration of Mouse Embryonic Fibroblasts through the β1 Integrin Pathway.
abstract: Protein 4.1G is a membrane skeletal protein that can serve as an adapter between transmembrane proteins and the underlying membrane skeleton. The function of 4.1G remains largely unexplored. Here, using 4.1G knockout mouse embryonic fibroblasts (MEFs) as a model system, we explored the function of 4.1G in motile cells. We show that the adhesion, spreading, and migration of 4.1G(-/-) MEF cells are impaired significantly. We further show that, although the total cellular expression of β1 integrin is unchanged, the surface expression of β1 integrin and its active form are decreased significantly in 4.1G(-/-) MEF cells. Moreover, the phosphorylation of focal adhesion kinase, a downstream component of the integrin-mediated signal transduction pathway, is suppressed in 4.1G(-/-) MEF cells. Co-immunoprecipitation experiments and in vitro binding assays showed that 4.1G binds directly to β1 integrin via its membrane-binding domain. These findings identified a novel role of 4.1G in cell adhesion, spreading, and migration in MEF cells by modulating the surface expression of β1 integrin and subsequent downstream signal transduction.
authors.affiliation: From the College of Life Science, Zhengzhou University, Science Road 100, Zhengzhou 450001, China, the Red Cell Physiology Laboratory and.
authors.forename: Lixiang
authors.initials: L
authors.lastname: Chen
authors.affiliation: From the College of Life Science, Zhengzhou University, Science Road 100, Zhengzhou 450001, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Wang
authors.affiliation: From the College of Life Science, Zhengzhou University, Science Road 100, Zhengzhou 450001, China.
authors.forename: Yaomei
authors.initials: Y
authors.lastname: Wang
authors.affiliation: From the College of Life Science, Zhengzhou University, Science Road 100, Zhengzhou 450001, China.
authors.forename: Jingxin
authors.initials: J
authors.lastname: Zhang
authors.affiliation: From the College of Life Science, Zhengzhou University, Science Road 100, Zhengzhou 450001, China.
authors.forename: Yuanming
authors.initials: Y
authors.lastname: Qi
authors.affiliation: From the College of Life Science, Zhengzhou University, Science Road 100, Zhengzhou 450001, China, Membrane Biology Laboratory, New York Blood Center, New York, New York 10065, and.
authors.forename: Haibo
authors.initials: H
authors.lastname: Weng
authors.affiliation: From the College of Life Science, Zhengzhou University, Science Road 100, Zhengzhou 450001, China.
authors.forename: Qiaozhen
authors.initials: Q
authors.lastname: Kang
authors.affiliation: the Red Cell Physiology Laboratory and.
authors.forename: Xinhua
authors.initials: X
authors.lastname: Guo
authors.affiliation: the School of Biosciences, University of Kent, Canterbury, Kent CT2 7NJ, United Kingdom.
authors.forename: Anthony J
authors.initials: AJ
authors.lastname: Baines
authors.affiliation: the Red Cell Physiology Laboratory and.
authors.forename: Narla
authors.initials: N
authors.lastname: Mohandas
authors.affiliation: From the College of Life Science, Zhengzhou University, Science Road 100, Zhengzhou 450001, China, Membrane Biology Laboratory, New York Blood Center, New York, New York 10065, and xan@nybloodcenter.org.
authors.forename: Xiuli
authors.initials: X
authors.lastname: An
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002465:Cell Movement; D003599:Cytoskeleton / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism*; D005434:Flow Cytometry; D051416:Focal Adhesion Protein-Tyrosine Kinases / Q000378:metabolism; D005786:Gene Expression Regulation; D005982:Glutathione Transferase / Q000378:metabolism; D019012:Integrin beta1 / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008840:Microfilament Proteins / Q000378:metabolism*; D010766:Phosphorylation; D017434:Protein Structure, Tertiary; D015398:Signal Transduction
chemical_list: C540651:Epb4.1l2 protein, mouse; D019012:Integrin beta1; D008840:Microfilament Proteins; D005982:Glutathione Transferase; D051416:Focal Adhesion Protein-Tyrosine Kinases
keywords: adhesion; cytoskeleton; focal adhesion kinase; membrane; migration; mouse embryonic, fibroblast; phosphorylation; protein 4.1G; spreading; β1 integrin