title: Glucocorticoid Signaling: An Update from a Genomic Perspective.
abstract: Glucocorticoid hormones (GC) regulate essential physiological functions including energy homeostasis, embryonic and postembryonic development, and the stress response. From the biomedical perspective, GC have garnered a tremendous amount of attention as highly potent anti-inflammatory and immunosuppressive medications indispensable in the clinic. GC signal through the GC receptor (GR), a ligand-dependent transcription factor whose structure, DNA binding, and the molecular partners that it employs to regulate transcription have been under intense investigation for decades. In particular, next-generation sequencing-based approaches have revolutionized the field by introducing a unified platform for a simultaneous genome-wide analysis of cellular activities at the level of RNA production, binding of transcription factors to DNA and RNA, and chromatin landscape and topology. Here we describe fundamental concepts of GC/GR function as established through traditional molecular and in vivo approaches and focus on the novel insights of GC biology that have emerged over the last 10 years from the rapidly expanding arsenal of system-wide genomic methodologies.
authors.affiliation: Hospital for Special Surgery, The David Rosensweig Genomics Center, New York, NY 10021; email: rogatskyi@hss.edu.|Weill Cornell/Rockefeller/Sloan Kettering MD/PhD program, New York, NY 10021.
authors.forename: Maria A
authors.initials: MA
authors.lastname: Sacta
authors.affiliation: Hospital for Special Surgery, The David Rosensweig Genomics Center, New York, NY 10021; email: rogatskyi@hss.edu.
authors.forename: Yurii
authors.initials: Y
authors.lastname: Chinenov
authors.affiliation: Hospital for Special Surgery, The David Rosensweig Genomics Center, New York, NY 10021; email: rogatskyi@hss.edu.|Weill Cornell/Rockefeller/Sloan Kettering MD/PhD program, New York, NY 10021.
authors.forename: Inez
authors.initials: I
authors.lastname: Rogatsky
mesh_terms: D000818:Animals; D016678:Genome / Q000235:genetics*; D023281:Genomics / Q000379:methods; D005938:Glucocorticoids / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D011965:Receptors, Glucocorticoid / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D005938:Glucocorticoids; D011965:Receptors, Glucocorticoid; D014157:Transcription Factors
keywords: genomic approaches; glucocorticoid receptor; next-generation sequencing; transcriptional regulation