title: Construction of a cytogenetic dose-response curve for low-dose range gamma-irradiation in human peripheral blood lymphocytes using three-color FISH.
abstract: In order to estimate biological doses after low-dose ionizing radiation exposure, fluorescence in situ hybridization (FISH) using three differentially colored chromosome painting probes was employed to detect exchange-type chromosome aberrations. A reference dose response curve was constructed using blood samples from a female donor whose lymphocytes consistently exhibited a low frequency of cells at the second mitosis under routine culture conditions. Aberration yields were studied for a total of about 155 thousand metaphases obtained from seven dose-points of gamma irradiations (0, 50, 100, 150, 200, 250 and 300mGy). In situ hybridization was performed using commercially available painting probes for chromosomes 1, 2 and 4. With the aid of an automated image-capturing method, exchange-type aberrations involving painted chromosomes were detected with considerable accuracy and speed. The results on the exchange-type aberrations (dicentrics plus translocations) at the seven dose-points showed a good fit to the linear-quadratic model (y=0.0023+0.0015x+0.0819x(2), P=0.83). A blind test proved the reproducibility of the reference dose-response relationship. In the control experiments using blood samples from another donor, the estimated doses calculated on the basis of the present reference curve were proved to be in good agreement with the actual physical doses applied. The present dose-response curve may serve as a means to assess the individual differences in cytogenetical radio-sensitivities.
authors.affiliation: Biodosimetry Research Team, Research Center for Radiation Emergency Medicine, National Institute of Radiological Sciences, Chiba, Japan. Electronic address: y-suto@nirs.go.jp.
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Suto
authors.affiliation: Biodosimetry Research Team, Research Center for Radiation Emergency Medicine, National Institute of Radiological Sciences, Chiba, Japan.
authors.forename: Miho
authors.initials: M
authors.lastname: Akiyama
authors.affiliation: REMAT, National Institute of Radiological Sciences, Chiba, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Noda
authors.affiliation: Biodosimetry Research Team, Research Center for Radiation Emergency Medicine, National Institute of Radiological Sciences, Chiba, Japan.
authors.forename: Momoki
authors.initials: M
authors.lastname: Hirai
mesh_terms: D000328:Adult; D002869:Chromosome Aberrations / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D005260:Female; D005720:Gamma Rays; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008214:Lymphocytes / Q000528:radiation effects*; D008677:Metaphase; D015203:Reproducibility of Results; D014178:Translocation, Genetic
keywords: 3-color FISH; Biodosimetry; Dose response curve; Gamma-ray irradiation; Human peripheral lymphocytes; Low-dose range