title: A novel fluorescent probe for Cr(3+) based on rhodamine-crown ether conjugate and its application to drinking water examination and bioimaging.
abstract: A trivalent chromium (Cr(3+)) fluorescence probe (RhC) was designed and synthesized via Schiff base reaction based on rhodamine-crown ether conjugate. This probe displayed a favorable selectivity for Cr(3+) over a range of other common metal ions in DMF/H2O (3:7, v/v; PBS buffer 50 mmol L(-1); pH=6.8) solution, leading to prominent fluorescence "OFF-ON" switching of the rhodamine fluorophore. The limit of detection was calculated to be 1.5 μmol L(-1) (S/N=3). The binding ratio of RhC-Cr(3+) complex was determined to be 1:2 according to the Job's plot and HR-MS. The probe was successfully applied to examination of Cr(3+) in drinking water spiked samples. The average recoveries ranged from 104.9% to 106.9% at spiked concentration level of 10.00 μmol L(-1), and the obtained results were consistent with those obtained using atomic absorption spectrometry (AAS). Moreover, bioimaging experiments showed that RhC can sense the Cr(3+) in living cells with a fluorescence enhancement signal.
authors.affiliation: College of Chemistry, Jilin University, Qianjin Street 2699, Changchun 130012, China; School of Chemistry and Life Science, Anshan Normal University, Ping'an Street 43, Anshan 114005, China.
authors.forename: Quanping
authors.initials: Q
authors.lastname: Diao
authors.affiliation: College of Chemistry, Jilin University, Qianjin Street 2699, Changchun 130012, China.
authors.forename: Pinyi
authors.initials: P
authors.lastname: Ma
authors.affiliation: School of Chemistry and Life Science, Anshan Normal University, Ping'an Street 43, Anshan 114005, China.
authors.forename: Linlin
authors.initials: L
authors.lastname: Lv
authors.affiliation: School of Chemistry and Life Science, Anshan Normal University, Ping'an Street 43, Anshan 114005, China.
authors.forename: Tiechun
authors.initials: T
authors.lastname: Li
authors.affiliation: College of Chemistry, Jilin University, Qianjin Street 2699, Changchun 130012, China.
authors.forename: Xinghua
authors.initials: X
authors.lastname: Wang
authors.affiliation: College of Chemistry, Jilin University, Qianjin Street 2699, Changchun 130012, China. Electronic address: songdq@jlu.edu.cn.
authors.forename: Daqian
authors.initials: D
authors.lastname: Song
mesh_terms: D002412:Cations / Q000032:analysis; D002857:Chromium / Q000032:analysis*; D043844:Crown Ethers / Q000737:chemistry*; D060766:Drinking Water / Q000032:analysis*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D057230:Limit of Detection; D061848:Optical Imaging; D012235:Rhodamines / Q000737:chemistry*; D012545:Schiff Bases / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D002412:Cations; D043844:Crown Ethers; D060766:Drinking Water; D005456:Fluorescent Dyes; D012235:Rhodamines; D012545:Schiff Bases; D014874:Water Pollutants, Chemical; D002857:Chromium
keywords: Bioimaging; Chromium (Cr(3+)); Drinking water; Fluorescent probe; Rhodamine