title: Molecular characterization of Newcastle disease virus strains isolated from different outbreaks in Northeast India during 2014-15.
abstract: Newcastle disease virus (NDV) isolates recovered from different outbreaks in chicken flocks in Assam during 2014-15 were genotypically and pathotypically characterized. Nucleotide sequence analysis of fusion (F) and hemagglutinin protein genes showed a close similarity with genotype XIII strains of NDV. Amino acid sequence of F protein showed a virulent cleavage site (112)R-R-Q-K-R-F(117). Furthermore, pathogenicity test in one-day-old chicks and embryonated chicken eggs showed a virulent pathotype of the isolated NDV strains. The study will help us to understand the biology of circulating strains of NDV in Northeastern part of India.
authors.affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, Assam 781039, India.
authors.forename: Barnali
authors.initials: B
authors.lastname: Nath
authors.affiliation: Department of Veterinary Microbiology, College of Veterinary Sciences, Assam Agricultural University, Khanapara, Guwahati, Assam 781022, India.
authors.forename: Nagendra N
authors.initials: NN
authors.lastname: Barman
authors.affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, Assam 781039, India. Electronic address: sachinku@iitg.ernet.in.
authors.forename: Sachin
authors.initials: S
authors.lastname: Kumar
mesh_terms: D000818:Animals; D002645:Chickens; D004196:Disease Outbreaks; D005838:Genotype; D007194:India / Q000453:epidemiology; D008969:Molecular Sequence Data; D009521:Newcastle Disease / Q000453:epidemiology / Q000821:virology*; D009522:Newcastle disease virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D010802:Phylogeny; D011201:Poultry Diseases / Q000453:epidemiology / Q000821:virology*
keywords: Newcastle disease virus; Outbreak; Pathogenicity; Virulent