title: Sulfation of benzyl alcohol by the human cytosolic sulfotransferases (SULTs): a systematic analysis.
abstract: The aim of the present study was to identify human cytosolic sulfotransferases (SULTs) that are capable of sulfating benzyl alcohol and to examine whether benzyl alcohol sulfation may occur in cultured human cells as well as in human organ homogenates. A systematic analysis revealed that of the 13 known human SULTs, SULT1A1 SULT1A2, SULTA3, and SULT1B1 are capable of mediating the sulfation of benzyl alcohol. The kinetic parameters of SULT1A1 that showed the strongest benzyl alcohol-sulfating activity were determined. HepG2 human hepatoma cells were used to demonstrate the generation and release of sulfated benzyl alcohol under the metabolic settings. Moreover, the cytosol or S9 fractions of human liver, lung, kidney and small intestine were examined to verify the presence of benzyl alcohol sulfating activity in vivo. Copyright © 2015 John Wiley & Sons, Ltd.
authors.affiliation: Department of Pharmacology, College of Pharmacy and Pharmaceutical Sciences, University of Toledo Health Science Campus, Toledo, OH, 43614, USA.
authors.forename: Lingtian
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Pharmacology, College of Pharmacy and Pharmaceutical Sciences, University of Toledo Health Science Campus, Toledo, OH, 43614, USA.|Biochemistry and Applied Biosciences, University of Miyazaki, Miyazaki, 889-2192, Japan.
authors.forename: Katsuhisa
authors.initials: K
authors.lastname: Kurogi
authors.affiliation: Department of Pharmacology, College of Pharmacy and Pharmaceutical Sciences, University of Toledo Health Science Campus, Toledo, OH, 43614, USA.|National Synchrotron Radiation Research Center, Hsinchu, Taiwan.
authors.forename: Ming-Yih
authors.initials: MY
authors.lastname: Liu
authors.affiliation: Department of Pharmacology, College of Pharmacy and Pharmaceutical Sciences, University of Toledo Health Science Campus, Toledo, OH, 43614, USA.
authors.forename: Alaina M
authors.initials: AM
authors.lastname: Schnapp
authors.affiliation: Department of Pharmacology, College of Pharmacy and Pharmaceutical Sciences, University of Toledo Health Science Campus, Toledo, OH, 43614, USA.
authors.forename: Frederick E
authors.initials: FE
authors.lastname: Williams
authors.affiliation: Biochemistry and Applied Biosciences, University of Miyazaki, Miyazaki, 889-2192, Japan.
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Sakakibara
authors.affiliation: Biochemistry and Applied Biosciences, University of Miyazaki, Miyazaki, 889-2192, Japan.
authors.forename: Masahito
authors.initials: M
authors.lastname: Suiko
authors.affiliation: Department of Pharmacology, College of Pharmacy and Pharmaceutical Sciences, University of Toledo Health Science Campus, Toledo, OH, 43614, USA.
authors.forename: Ming-Cheh
authors.initials: MC
authors.lastname: Liu
mesh_terms: D015239:Arylsulfotransferase / Q000378:metabolism*; D019905:Benzyl Alcohol / Q000633:toxicity*; D018938:Caco-2 Cells; D003600:Cytosol / Q000187:drug effects* / Q000378:metabolism; D056945:Hep G2 Cells; D006801:Humans; D007421:Intestine, Small / Q000187:drug effects / Q000378:metabolism; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008168:Lung / Q000187:drug effects / Q000378:metabolism; D013431:Sulfates / Q000378:metabolism
chemical_list: D013431:Sulfates; D015239:Arylsulfotransferase; C117544:SULT1A1 protein, human; D019905:Benzyl Alcohol
keywords: SULT; benzyl alcohol; cytosolic sulfotransferase; human; sulfation