title: PEGylated silica-enzyme nanoconjugates: a new frontier in large scale separation of α-amylase.
abstract: High resolution is nearly lost at the expense of throughput in most conventional bioseparation methods. Nanoparticles, due to their high surface to volume ratio, are attractiveenzyme carriers, which can boost the performance of extraction manifold. Here, wereport design and application ofa method highly capable of improving the partitioning of α-amylase in aqueous two-phase system of polymer and salt. Silica nanoparticle introduced to the system acts as a bridge that connects the enzyme and polymer. Theconjugated nanoparticles form the major part of the upper phase and thus significantly enhance the protein recovery. A thorough investigation was performed on the structure of the nanoconjugatesas well as analyzing the conformational structure of the enzyme after conjugationto explore anypossible denaturation.
authors.affiliation: Institute for Nanoscience and Nanotechnology, Sharif University of Technology, Tehran, Iran.
authors.forename: Seyed
authors.initials: S
authors.lastname: Mohsen Dehnavi
authors.affiliation: Department of Chemical Engineering, Amirkabir University of Technology (Tehran Polytechnic), Tehran, Iran.
authors.forename: Gholamreza
authors.initials: G
authors.lastname: Pazuki
authors.affiliation: Institute for Nanoscience and Nanotechnology, Sharif University of Technology, Tehran, Iran.|Department of Chemical and Petroleum Engineering, Sharif University of Technology, Tehran, Iran.
authors.forename: Manouchehr
authors.initials: M
authors.lastname: Vossoughi
mesh_terms: D004795:Enzyme Stability; D059625:Liquid-Liquid Extraction / Q000379:methods; D058726:Nanoconjugates; D011092:Polyethylene Glycols / Q000737:chemistry; D011487:Protein Conformation; D012822:Silicon Dioxide / Q000737:chemistry; D000516:alpha-Amylases / Q000737:chemistry / Q000302:isolation & purification* / Q000648:ultrastructure
chemical_list: D058726:Nanoconjugates; D011092:Polyethylene Glycols; D012822:Silicon Dioxide; D000516:alpha-Amylases