title: High-yield production of canine parvovirus virus-like particles in a baculovirus expression system.
abstract: An optimized VP2 gene from the current prevalent CPV strain (new CPV-2a) in China was expressed in a baculovirus expression system. It was found that the VP2 proteins assembled into virus-like particles (VLPs) with antigenic properties similar to those of natural CPV and with an especially high hemagglutination (HA) titer (1:2(20)). Dogs intramuscularly or orally immunized with VLPs produced antibodies against CPV with >1:80 hemagglutination inhibition (HI) units for at least 3 months. The CPV VLPs could be considered for use as a vaccine against CPV or as a platform for research on chimeric VLP vaccines against other diseases.
authors.affiliation: Changchun SR Biological Technology Co., LTD, Changchun, 130012, China. jin8616771@163.com.
authors.forename: Hongli
authors.initials: H
authors.lastname: Jin
authors.affiliation: Changchun SR Biological Technology Co., LTD, Changchun, 130012, China.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Xia
authors.affiliation: Changchun SR Biological Technology Co., LTD, Changchun, 130012, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Liu
authors.affiliation: Changchun SR Biological Technology Co., LTD, Changchun, 130012, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Fu
authors.affiliation: Changchun SR Biological Technology Co., LTD, Changchun, 130012, China.
authors.forename: Xianping
authors.initials: X
authors.lastname: Chen
authors.affiliation: Changchun SR Biological Technology Co., LTD, Changchun, 130012, China.
authors.forename: Huihui
authors.initials: H
authors.lastname: Wang
authors.affiliation: Changchun SR Biological Technology Co., LTD, Changchun, 130012, China. ccxn01@hotmail.com.
authors.forename: Zhenqiang
authors.initials: Z
authors.lastname: Xia
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D016367:Baculoviridae; D002681:China; D004285:Dogs; D005822:Genetic Vectors; D007273:Injections, Intramuscular; D017993:Parvovirus, Canine / Q000235:genetics*; D055503:Protein Multimerization; D022721:Vaccines, Virosome / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D015678:Viral Structural Proteins / Q000235:genetics / Q000378:metabolism*; D014765:Viral Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D022701:Virosomes / Q000235:genetics / Q000378:metabolism*
chemical_list: D000914:Antibodies, Viral; D022721:Vaccines, Virosome; D015678:Viral Structural Proteins; D014765:Viral Vaccines; D022701:Virosomes
keywords: Canine parvovirus; Vaccine; Virus-like particles