title: Expression and colocalization of NMDA receptor and FosB/ΔFosB in sensitive brain regions in rats after chronic morphine exposure.
abstract: Research in the last decade demonstrated that the NMDA receptor (NMDAR) has an important role in opiate-induced neural and behavioral plasticity. In addition, increased levels of FosB-like proteins (FosB/ΔFosB) were found to be related to morphine withdrawal behaviors. However, the relationship between NMDAR and FosB/ΔFosB in sensitive brain regions during morphine withdrawal is largely unknown. In this study, we aimed to investigate NMDAR dynamics and FosB/ΔFosB levels in multiple brain regions and whether they are related in sensitive brain regions during morphine abstinence. Quantitative immunohistochemistry was adopted to test NMDAR and FosB/ΔfosB levels during morphine withdrawal in rats. Increased NMDAR and FosB/ΔFosB levels were found in the nucleus accumbens core (AcbC), nucleus accumbens shell (AcbSh), central amygdaloid nucleuscapsular part (CeC), ventral tegmental area (VTA) and cingulate cortex (Cg). Double-immunofluorescence labeling indicated that NMDAR colocalized with Fos/ΔFosB in these five regions. These results suggest that multiple phenotypic regions are mediated by NMDAR and Fos/ΔFosB during morphine withdrawal, such as the motivational (AcbC, AcbSh), limbic (CeC, VTA) and executive (Cg) system pathways, and may be the primary targets of NMDAR and Fos/ΔfosB that impact morphine withdrawal behaviors.
authors.affiliation: Department of Genetics, Xuzhou Medical College, Xuzhou City, PR China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Graduate school, Xuzhou Medical College, Xuzhou City, PR China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Graduate school, Xuzhou Medical College, Xuzhou City, PR China.
authors.forename: Tongzhou
authors.initials: T
authors.lastname: Li
authors.affiliation: Graduate school, Xuzhou Medical College, Xuzhou City, PR China.
authors.forename: You
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Institute of Audiology and Speech Science of Xuzhou Medical College, Xuzhou City, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: Research Facility Center for Morphology of Xuzhou Medical College, Xuzhou City, PR China.
authors.forename: Zhonghai
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Research Facility Center for Morphology of Xuzhou Medical College, Xuzhou City, PR China.
authors.forename: Hongzhi
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Ophthalmology, The Second People's Hospital of Yunnan Province, Kunming City, PR China. Electronic address: fudanhumin@sina.com.
authors.forename: Min
authors.initials: M
authors.lastname: Hu
authors.affiliation: Institute of Audiology and Speech Science of Xuzhou Medical College, Xuzhou City, PR China. Electronic address: oto8558@163.com.
authors.forename: Yuehua
authors.initials: Y
authors.lastname: Qiao
authors.affiliation: Department of Genetics, Xuzhou Medical College, Xuzhou City, PR China; Institute of Audiology and Speech Science of Xuzhou Medical College, Xuzhou City, PR China. Electronic address: nhcnhc@163.com.
authors.forename: Haichen
authors.initials: H
authors.lastname: Niu
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D009020:Morphine / Q000009:adverse effects*; D009294:Narcotics / Q000009:adverse effects*; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism*; D017207:Rats, Sprague-Dawley; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism*; D013375:Substance Withdrawal Syndrome / Q000378:metabolism* / Q000523:psychology
chemical_list: C483820:Fosb protein, rat; C409466:NR1 NMDA receptor; D009294:Narcotics; D016760:Proto-Oncogene Proteins c-fos; D016194:Receptors, N-Methyl-D-Aspartate; D009020:Morphine
keywords: FosB proteins; Limbic system; Morphine; NMDA receptor; Withdrawal