title: Tube Radial Distribution Chromatography on a Microchip Incorporating Microchannels with a Three-to-One Channel Confluence Point.
abstract: We developed a capillary chromatography system using a phase-separated solvent mixture as a carrier solution--i.e., a water-hydrophilic/hydrophobic organic solvent mixture--which we call "tube radial distribution chromatography" (TRDC). Here, we attempted to apply the TRDC system to a microchip incorporating microchannels with a double T-junction for injection of analyte solution and a three-to-one, narrow-to-wide channel confluence point for tube radial distribution phenomenon (TRDP) at room temperature. A ternary mixed solvent of water, acetonitrile and ethyl acetate was used as a carrier solution. TRDP in the wide microchannel was examined using various flow rates, temperatures, and component solvent ratios. Successful observation was carried out using a fluorescence microscope-CCD camera. Model analytes perylene (hydrophobic) and Eosin Y (hydrophilic) were separated by flowing through the microchannel, without any treatment such as packed columns or coating, at room temperature (25°C).
authors.affiliation: Department of Chemical Engineering and Materials Science, Doshisha University.
authors.forename: Naomichi
authors.initials: N
authors.lastname: Suzuki
authors.forename: Kenichi
authors.initials: K
authors.lastname: Yamashita
authors.forename: Hideaki
authors.initials: H
authors.lastname: Maeda
authors.forename: Masahiko
authors.initials: M
authors.lastname: Hashimoto
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Tsukagoshi