title: Survivin, cyclin D1, and p21hras in keratocystic odontogenic tumors before and after decompression.
abstract: OBJECTIVE
  The aim of this study was to investigate survivin, cyclin D1, and p21hras expression in keratocystic odontogenic tumors before and after decompression, as well as in pericoronal follicles. A potential correlation between the expression levels of these proteins was also investigated.
  
  
  METHODS
  We analyzed eighteen keratocystic tumors treated by decompression and subsequent enucleation along with seven pericoronal follicles using immunohistochemistry.
  
  
  RESULTS
  Keratocystic tumor samples, both before and after decompression, were positive for each of the investigated proteins. In pericoronal follicles, survivin exhibited cytoplasmic staining in contrast to nuclear staining in keratocystic tumors. Cyclin D1 expression was negative in pericoronal follicles, and p21hras expression was similar in both groups. Survivin showed significantly higher expression after decompression, while cyclin D1 and p21hras remained unchanged (P = 0.039, P = 0.255, P = 0.913, respectively). There was no correlation between these proteins neither before nor after decompression.
  
  
  CONCLUSIONS
  Within the limits of the study, we can conclude that following decompression, keratocystic odontogenic tumors preserve distinct immunohistochemical profiles of cyclin D1 and p21hras expression, despite substantial reduction in size of the lesions. Significant increase of survivin expression after decompression might be attributed to higher level of epithelial proliferation caused by this procedure.
authors.affiliation: Department of Oral Surgery, School of Dental Medicine, University of Belgrade, Belgrade, Serbia.
authors.forename: I
authors.identifier: http://orcid.org/0000-0002-7366-1171
authors.initials: I
authors.lastname: Brajić
authors.affiliation: Department of Pathology, School of Medicine, University of Belgrade, Belgrade, Serbia.
authors.forename: S
authors.initials: S
authors.lastname: Škodrić
authors.affiliation: Department of Pathology, Clinical Hospital Center, Zemun, Serbia.
authors.forename: S
authors.initials: S
authors.lastname: Milenković
authors.affiliation: Department of Pathology, School of Dental Medicine, University of Belgrade, Belgrade, Serbia.
authors.forename: Z
authors.initials: Z
authors.lastname: Tepavčević
authors.affiliation: School of Medicine, University of Belgrade, Belgrade, Serbia.
authors.forename: I
authors.initials: I
authors.lastname: Soldatović
authors.affiliation: Department of Oral Surgery, School of Dental Medicine, University of Belgrade, Belgrade, Serbia.
authors.forename: S
authors.initials: S
authors.lastname: Čolić
authors.affiliation: Department of Molecular Genetics, School of Dental Medicine, University of Belgrade, Belgrade, Serbia.
authors.forename: J
authors.initials: J
authors.lastname: Milašin
authors.affiliation: Department of Oral Surgery, School of Dental Medicine, University of Belgrade, Belgrade, Serbia.
authors.forename: M
authors.initials: M
authors.lastname: Andrić
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D019938:Cyclin D1 / Q000378:metabolism*; D019299:Decompression, Surgical; D005260:Female; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000378:metabolism*; D008297:Male; D008339:Mandibular Neoplasms / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D008441:Maxillary Neoplasms / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D008875:Middle Aged; D009807:Odontogenic Cysts / Q000473:pathology; D009808:Odontogenic Tumors / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D016283:Proto-Oncogene Proteins p21(ras) / Q000378:metabolism*; D000077022:Survivin; D055815:Young Adult
chemical_list: C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D000077022:Survivin; D019938:Cyclin D1; C501009:HRAS protein, human; D016283:Proto-Oncogene Proteins p21(ras)
keywords: cyclin D1; decompression; keratocystic odontogenic tumor; p21hras; survivin