title: Lack of association between the CARD10 rs6000782 polymorphism and type 1 autoimmune hepatitis in a Japanese population.
abstract: BACKGROUND
  Previous genome-wide association studies have evaluated the impact of common genetic variants and identified several non-HLA risk loci associated with autoimmune liver diseases. More recent genome-wide association studies and replication analyses reported an association between variants of the CARD10 polymorphism rs6000782 and risk of type 1 autoimmune hepatitis (AIH). In this case-control study, we genotyped 326 Japanese AIH patients and 214 control subjects.
  
  
  RESULTS
  Genomic DNA from 540 individuals of Japanese origin, including 326 patients with type-1 AIH and 214 healthy controls, was analyzed for two single nucleotide polymorphisms (SNPs) in the CARD10 gene. We selected CARD10 rs6000782 SNPs and genotyped these using PCR-RFLP method and direct sequencing. The Chi square test revealed that the rs6000782 variant alle (c) was not associated with the susceptibility for AIH in a Japanese population [p = 0.376, odds ratio (OR) 1.271, 95 % confidence interval (CI) 0.747-2.161] in an allele model. Our data also showed that CARD10 rs6000782 variants were not associated with AIH or with the clinical parameters of AIH.
  
  
  CONCLUSIONS
  In this study we examined an association between rs6000782 SNPs in the CARD10 gene and type-1 AIH. Results showed no significant association of rs62000782 with type-1 AIH in a Japanese population. This study demonstrated no association between CARD10 rs6000782 variants and AIH in a Japanese population.
authors.affiliation: NHO-AIH study group, Nagasaki Medical Center, Kubara 2-1001-1, Omura, Nagasaki, 856-8562, Japan. migita@nagasaki-mc.com.|Clinical Research Center, NHO Nagasaki Medical Center, Kubara 2-1001-1, Omura, 856-8652, Japan. migita@nagasaki-mc.com.
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Migita
authors.affiliation: NHO-AIH study group, Nagasaki Medical Center, Kubara 2-1001-1, Omura, Nagasaki, 856-8562, Japan. jiuchi@nagasaki-mc.com.
authors.forename: Yuka
authors.initials: Y
authors.lastname: Jiuchi
authors.affiliation: Department of Rheumatology, NHO Sagamihara Hospital, Minamikusakuradai 18-1, Sagamihara, Kanagawa, 252-0392, Japan. furukawa-tky@umin.org.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Furukawa
authors.affiliation: Department of Hepatology, Nagasaki University Graduate School of Biomedical Sciences, Sakamoto 1-12-4, Ngasaki, Nagasaki, 852-8523, Japan. nakamuram@nagasaki-mc.com.
authors.forename: Minoru
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authors.affiliation: NHO-AIH study group, Nagasaki Medical Center, Kubara 2-1001-1, Omura, Nagasaki, 856-8562, Japan. komori@nagasaki-mc.com.
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authors.affiliation: Department of Clinical Medicine, Institute of Tropical Medicine, Nagasaki University, Sakamoto 1-12-4, Ngasaki, Nagasaki, 852-8523, Japan. yasunami@nagasaki-u.ac.jp.
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authors.affiliation: NHO-AIH study group, Nagasaki Medical Center, Kubara 2-1001-1, Omura, Nagasaki, 856-8562, Japan. hkozuru@nagasaki-mc.com.
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authors.affiliation: NHO-AIH study group, Nagasaki Medical Center, Kubara 2-1001-1, Omura, Nagasaki, 856-8562, Japan. s.abiru@nagasaki-mc.com.
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authors.affiliation: NHO-AIH study group, Nagasaki Medical Center, Kubara 2-1001-1, Omura, Nagasaki, 856-8562, Japan. k.yamasaki@nagasaki-mc.com.
authors.forename: Kazumi
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authors.affiliation: NHO-AIH study group, Nagasaki Medical Center, Kubara 2-1001-1, Omura, Nagasaki, 856-8562, Japan. sinagaoka@nagasaki-mc.com.
authors.forename: Shinya
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authors.affiliation: NHO-AIH study group, Nagasaki Medical Center, Kubara 2-1001-1, Omura, Nagasaki, 856-8562, Japan. shashimoto@nagasaki-mc.com.
authors.forename: Satoru
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authors.affiliation: NHO-AIH study group, Nagasaki Medical Center, Kubara 2-1001-1, Omura, Nagasaki, 856-8562, Japan. bekki@nagasaki-mc.com.
authors.forename: Shigemune
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authors.lastname: Bekki
authors.affiliation: NHO-AIH study group, Nagasaki Medical Center, Kubara 2-1001-1, Omura, Nagasaki, 856-8562, Japan. k.yoshizawa@nagano-hosp.go.jp.
authors.forename: Kaname
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authors.affiliation: NHO-AIH study group, Nagasaki Medical Center, Kubara 2-1001-1, Omura, Nagasaki, 856-8562, Japan. shimada@nnh.hosp.go.jp.
authors.forename: Masaaki
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authors.affiliation: NHO-AIH study group, Nagasaki Medical Center, Kubara 2-1001-1, Omura, Nagasaki, 856-8562, Japan. hkouno@kure-nh.go.jp.
authors.forename: Hiroshi
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authors.affiliation: NHO-AIH study group, Nagasaki Medical Center, Kubara 2-1001-1, Omura, Nagasaki, 856-8562, Japan. kamihiro-in@tokyo-hosp.jp.
authors.forename: Hiroshi
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authors.affiliation: NHO-AIH study group, Nagasaki Medical Center, Kubara 2-1001-1, Omura, Nagasaki, 856-8562, Japan. tkoma@hosp.go.jp.
authors.forename: Tatsuji
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authors.affiliation: NHO-AIH study group, Nagasaki Medical Center, Kubara 2-1001-1, Omura, Nagasaki, 856-8562, Japan. hijioka@ommc-hp.jp.
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authors.affiliation: NHO-AIH study group, Nagasaki Medical Center, Kubara 2-1001-1, Omura, Nagasaki, 856-8562, Japan. nakamuta@kyumed.jp.
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authors.affiliation: NHO-AIH study group, Nagasaki Medical Center, Kubara 2-1001-1, Omura, Nagasaki, 856-8562, Japan. numackey@pa2.so-net.ne.jp.
authors.forename: Atsushi
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authors.affiliation: NHO-AIH study group, Nagasaki Medical Center, Kubara 2-1001-1, Omura, Nagasaki, 856-8562, Japan. haruymst@okayama3.hosp.go.jp.
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authors.affiliation: NHO-AIH study group, Nagasaki Medical Center, Kubara 2-1001-1, Omura, Nagasaki, 856-8562, Japan. nishim-1@hosp.go.jp.
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authors.affiliation: NHO-AIH study group, Nagasaki Medical Center, Kubara 2-1001-1, Omura, Nagasaki, 856-8562, Japan. h-ohta@kinbyou.hosp.go.jp.
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authors.affiliation: NHO-AIH study group, Nagasaki Medical Center, Kubara 2-1001-1, Omura, Nagasaki, 856-8562, Japan. nakamury-1@hosp.go.jp.
authors.forename: Yoko
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authors.affiliation: NHO-AIH study group, Nagasaki Medical Center, Kubara 2-1001-1, Omura, Nagasaki, 856-8562, Japan. ariok@uresino.go.jp.
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authors.affiliation: NHO-AIH study group, Nagasaki Medical Center, Kubara 2-1001-1, Omura, Nagasaki, 856-8562, Japan. ooharay@hok-mc.hosp.go.jp.
authors.forename: Yukio
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authors.affiliation: NHO-AIH study group, Nagasaki Medical Center, Kubara 2-1001-1, Omura, Nagasaki, 856-8562, Japan. sugi@kumamed.jp.
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authors.affiliation: NHO-AIH study group, Nagasaki Medical Center, Kubara 2-1001-1, Omura, Nagasaki, 856-8562, Japan. nihminor-cib@umin.ac.jp.
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authors.affiliation: NHO-AIH study group, Nagasaki Medical Center, Kubara 2-1001-1, Omura, Nagasaki, 856-8562, Japan. satot@kokura2.hosp.go.jp.
authors.forename: Takeaki
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authors.affiliation: NHO-AIH study group, Nagasaki Medical Center, Kubara 2-1001-1, Omura, Nagasaki, 856-8562, Japan. dr.hr.tkhs.meddr@s8.dion.ne.jp.
authors.forename: Hironao
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authors.affiliation: NHO-AIH study group, Nagasaki Medical Center, Kubara 2-1001-1, Omura, Nagasaki, 856-8562, Japan. muro@oita2.hosp.go.jp.
authors.forename: Toyokichi
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authors.affiliation: NHO-AIH study group, Nagasaki Medical Center, Kubara 2-1001-1, Omura, Nagasaki, 856-8562, Japan. makitaf@netnngh.hosp.go.jp.
authors.forename: Fujio
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authors.affiliation: NHO-AIH study group, Nagasaki Medical Center, Kubara 2-1001-1, Omura, Nagasaki, 856-8562, Japan. eijim67@onh.go.jp.
authors.forename: Eiji
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authors.affiliation: NHO-AIH study group, Nagasaki Medical Center, Kubara 2-1001-1, Omura, Nagasaki, 856-8562, Japan. hsakai@hosp.go.jp.
authors.forename: Hironori
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authors.affiliation: NHO-AIH study group, Nagasaki Medical Center, Kubara 2-1001-1, Omura, Nagasaki, 856-8562, Japan. yatsuhashi@nagasaki-mc.com.
authors.forename: Hiroshi
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mesh_terms: D000368:Aged; D044466:Asian People / Q000235:genetics; D001483:Base Sequence; D053468:CARD Signaling Adaptor Proteins / Q000235:genetics*; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease / Q000208:ethnology / Q000235:genetics*; D005838:Genotype; D019693:Hepatitis, Autoimmune / Q000145:classification / Q000208:ethnology / Q000235:genetics*; D006801:Humans; D007564:Japan; D015810:Linkage Disequilibrium; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors; D017422:Sequence Analysis, DNA
chemical_list: D053468:CARD Signaling Adaptor Proteins; C430880:CARD10 protein, human