title: Innate control of actin nucleation determines two distinct migration behaviours in dendritic cells.
abstract: Dendritic cell (DC) migration in peripheral tissues serves two main functions: antigen sampling by immature DCs, and chemokine-guided migration towards lymphatic vessels (LVs) on maturation. These migratory events determine the efficiency of the adaptive immune response. Their regulation by the core cell locomotion machinery has not been determined. Here, we show that the migration of immature DCs depends on two main actin pools: a RhoA-mDia1-dependent actin pool located at their rear, which facilitates forward locomotion; and a Cdc42-Arp2/3-dependent actin pool present at their front, which limits migration but promotes antigen capture. Following TLR4-MyD88-induced maturation, Arp2/3-dependent actin enrichment at the cell front is markedly reduced. Consequently, mature DCs switch to a faster and more persistent mDia1-dependent locomotion mode that facilitates chemotactic migration to LVs and lymph nodes. Thus, the differential use of actin-nucleating machineries optimizes the migration of immature and mature DCs according to their specific function.
authors.affiliation: Inserm U932, Institut Curie, 12 rue Lhomond, 75005 Paris, France.|CNRS UMR144, Institut Curie, 12 rue Lhomond, 75005 Paris, France.
authors.forename: Pablo
authors.initials: P
authors.lastname: Vargas
authors.affiliation: CNRS UMR144, Institut Curie, 12 rue Lhomond, 75005 Paris, France.
authors.forename: Paolo
authors.initials: P
authors.lastname: Maiuri
authors.affiliation: Inserm U932, Institut Curie, 12 rue Lhomond, 75005 Paris, France.
authors.forename: Marine
authors.initials: M
authors.lastname: Bretou
authors.affiliation: Inserm U932, Institut Curie, 12 rue Lhomond, 75005 Paris, France.
authors.forename: Pablo J
authors.initials: PJ
authors.lastname: Sáez
authors.affiliation: Inserm U932, Institut Curie, 12 rue Lhomond, 75005 Paris, France.
authors.forename: Paolo
authors.initials: P
authors.lastname: Pierobon
authors.affiliation: Inserm U932, Institut Curie, 12 rue Lhomond, 75005 Paris, France.
authors.forename: Mathieu
authors.initials: M
authors.lastname: Maurin
authors.affiliation: Inserm U932, Institut Curie, 12 rue Lhomond, 75005 Paris, France.
authors.forename: Mélanie
authors.initials: M
authors.lastname: Chabaud
authors.affiliation: Inserm U932, Institut Curie, 12 rue Lhomond, 75005 Paris, France.
authors.forename: Danielle
authors.initials: D
authors.lastname: Lankar
authors.affiliation: Inserm U932, Institut Curie, 12 rue Lhomond, 75005 Paris, France.
authors.forename: Dorian
authors.initials: D
authors.lastname: Obino
authors.affiliation: CNRS UMR144, Institut Curie, 12 rue Lhomond, 75005 Paris, France.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Terriac
authors.affiliation: CNRS UMR144, Institut Curie, 12 rue Lhomond, 75005 Paris, France.
authors.forename: Matthew
authors.initials: M
authors.lastname: Raab
authors.affiliation: CNRS UMR144, Institut Curie, 12 rue Lhomond, 75005 Paris, France.
authors.forename: Hawa-Racine
authors.initials: HR
authors.lastname: Thiam
authors.affiliation: Institute for Immunology, Ludwig-Maximilian University of Munich, 80336 Munich, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Brocker
authors.affiliation: Van Andel Research Institute, 333 Bostwick Avenue N.E., Grand Rapids, Michigan 49503, USA.
authors.forename: Susan M
authors.initials: SM
authors.lastname: Kitchen-Goosen
authors.affiliation: Van Andel Research Institute, 333 Bostwick Avenue N.E., Grand Rapids, Michigan 49503, USA.
authors.forename: Arthur S
authors.initials: AS
authors.lastname: Alberts
authors.affiliation: Johns Hopkins University School of Medicine, 855 N. Wolfe Street, Baltimore, Maryland 21205, USA.
authors.forename: Praveen
authors.initials: P
authors.lastname: Sunareni
authors.affiliation: Johns Hopkins University School of Medicine, 855 N. Wolfe Street, Baltimore, Maryland 21205, USA.
authors.forename: Sheng
authors.initials: S
authors.lastname: Xia
authors.affiliation: Johns Hopkins University School of Medicine, 855 N. Wolfe Street, Baltimore, Maryland 21205, USA.
authors.forename: Rong
authors.initials: R
authors.lastname: Li
authors.affiliation: CNRS UMR 7600, Université Pierre et Marie Curie, 4 Place Jussieu, 75005 Paris, France.|CNRS FRE 3231, Université Pierre et Marie Curie, 4 Place Jussieu, 75005 Paris, France.
authors.forename: Raphael
authors.initials: R
authors.lastname: Voituriez
authors.affiliation: CNRS UMR144, Institut Curie, 12 rue Lhomond, 75005 Paris, France.
authors.forename: Matthieu
authors.initials: M
authors.lastname: Piel
authors.affiliation: Inserm U932, Institut Curie, 12 rue Lhomond, 75005 Paris, France.
authors.forename: Ana-Maria
authors.identifier: http://orcid.org/0000-0003-4383-1034
authors.initials: AM
authors.lastname: Lennon-Duménil
mesh_terms: D000199:Actins / Q000378:metabolism*; D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D002465:Cell Movement / Q000502:physiology*; D002478:Cells, Cultured; D002633:Chemotaxis / Q000502:physiology*; D003713:Dendritic Cells / Q000378:metabolism*; D051379:Mice
chemical_list: D000199:Actins