title: The Impact of Ozone Treatment in Dynamic Bed Parameters on Changes in Biologically Active Substances of Juniper Berries.
abstract: The development of the parameters of ozone decontamination method assuring the least possible losses of biologically active substances (essential oils and polyphenols) and their activity in common juniper (Juniperus communis (L.)) berries was studied. Ozone treatment in dynamic bed was conducted 9 times. The process was conducted under different ozone concentrations (100.0; 130.0; 160.0 g O3/m3) and times (30, 60, 90 min). After each decontamination, the microbiological profile of the juniper berries was studied, and the contaminating microflora was identified. Next to the microbiological profile, the phenolic profile, as well as antioxidant activity of extracts and essential oils were determined. The total polyphenol content (TPC), composition of essential oils, free radical-scavenging capacity, total antioxidant capacity, ferric-reducing antioxidant power (FRAP), beta-carotene bleaching test (BCB) and LC-MS polyphenol analysis were carried out. The study reveals that during short ozone contact times, higher amounts of TPC, 15.47 and 12.91 mg CE/g of extract, for samples 100/30 and 130/30, respectively, were demonstrated. Whereas samples 100/60, 130/60, 100/90, and 160/90 exhibited the lowest amount of phenolics. The highest antioxidant activity was found in the methanol extract obtained from ozonated berries which exhibited the lowest IC50 in all the antioxidant assays, such as DPPH, FRAP, and BCB assays. Ozone treatment showed noteworthy potential and its usage in food manufacturing and as an alternative decontamination method should be considered.
authors.affiliation: Institute of General Food Chemistry, Faculty of Biotechnology and Food Sciences, Lodz University of Technology, Lodz, Poland.
authors.forename: Agnieszka Joanna
authors.initials: AJ
authors.lastname: Brodowska
authors.affiliation: Institute of General Food Chemistry, Faculty of Biotechnology and Food Sciences, Lodz University of Technology, Lodz, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Śmigielski
authors.affiliation: Institute of Fermentation Technology and Microbiology, Faculty of Biotechnology and Food Sciences, Lodz University of Technology, Lodz, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Nowak
authors.affiliation: Institute of Fermentation Technology and Microbiology, Faculty of Biotechnology and Food Sciences, Lodz University of Technology, Lodz, Poland.
authors.forename: Agata
authors.initials: A
authors.lastname: Czyżowska
authors.affiliation: Institute of Fermentation Technology and Microbiology, Faculty of Biotechnology and Food Sciences, Lodz University of Technology, Lodz, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Otlewska
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification*; D001713:Biphenyl Compounds / Q000037:antagonists & inhibitors; D005511:Food Handling / Q000379:methods; D005638:Fruit / Q000737:chemistry / Q000187:drug effects* / Q000382:microbiology; D006801:Humans; D018653:Juniperus / Q000737:chemistry / Q000187:drug effects / Q000382:microbiology; D009822:Oils, Volatile / Q000302:isolation & purification*; D010084:Oxidation-Reduction; D010126:Ozone / Q000494:pharmacology*; D010851:Picrates / Q000037:antagonists & inhibitors; D010936:Plant Extracts / Q000737:chemistry; D059808:Polyphenols / Q000302:isolation & purification*; D013997:Time Factors; D019207:beta Carotene / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D000890:Anti-Infective Agents; D000975:Antioxidants; D001713:Biphenyl Compounds; D009822:Oils, Volatile; D010851:Picrates; D010936:Plant Extracts; D059808:Polyphenols; D019207:beta Carotene; D010126:Ozone; C004931:1,1-diphenyl-2-picrylhydrazyl