title: Yersinia Type III Secretion System Master Regulator LcrF.
abstract: Many Gram-negative pathogens express a type III secretion (T3SS) system to enable growth and survival within a host. The three human-pathogenic Yersinia species, Y. pestis, Y. pseudotuberculosis, and Y. enterocolitica, encode the Ysc T3SS, whose expression is controlled by an AraC-like master regulator called LcrF. In this review, we discuss LcrF structure and function as well as the environmental cues and pathways known to regulate LcrF expression. Similarities and differences in binding motifs and modes of action between LcrF and the Pseudomonas aeruginosa homolog ExsA are summarized. In addition, we present a new bioinformatics analysis that identifies putative LcrF binding sites within Yersinia target gene promoters.
authors.affiliation: Department of Molecular, Cell, and Developmental Biology, University of California, Santa Cruz, Santa Cruz, California, USA.
authors.forename: Leah
authors.initials: L
authors.lastname: Schwiesow
authors.affiliation: Department of Microbiology and Environmental Toxicology, University of California, Santa Cruz, Santa Cruz, California, USA.
authors.forename: Hanh
authors.initials: H
authors.lastname: Lam
authors.affiliation: Department of Molecular Infection Biology, Helmholtz Center for Infection Research, Braunschweig, Lower Saxony, Germany petra.dersch@helmholtz-hzi.de vastone@ucsc.edu.
authors.forename: Petra
authors.initials: P
authors.lastname: Dersch
authors.affiliation: Department of Microbiology and Environmental Toxicology, University of California, Santa Cruz, Santa Cruz, California, USA petra.dersch@helmholtz-hzi.de vastone@ucsc.edu.
authors.forename: Victoria
authors.initials: V
authors.lastname: Auerbuch
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D017434:Protein Structure, Tertiary; D015534:Trans-Activators / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000069296:Type III Secretion Systems / Q000235:genetics* / Q000378:metabolism; D015007:Yersinia / Q000737:chemistry / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D015534:Trans-Activators; D000069296:Type III Secretion Systems; C075515:lcrF protein, Yersinia pestis