title: Retinoic acid and retinaldehyde dehydrogenase are not involved in the specific induction of the follicle-stimulating hormone β subunit by trichostatin A, a selective inhibitor of histone deacetylase.
abstract: The selective histone deacetylase inhibitor, trichostatin A (TSA), increases follicle-stimulating hormone β subunit (FSHβ) mRNA expression but not α- and luteinizing hormone β (LHβ)-subunits in both the pituitary gonadotrophic cell line LβT2 and primary cultures of rat anterior pituitary cells. TSA increased histone acetylation in whole cell lysates in both cells. In addition, retinaldehyde dehydrogenases (RALDHs), which are retinoic acid (RA)-synthesizing enzymes, were induced by TSA in these cells. Anacardic acid, a histone acetyltransferase inhibitor that prevents histone acetylation, significantly inhibited TSA-induced FSHβ mRNA expression as well as TSA-induced RALDH2 and RALDH3 mRNA expression. Similar to the effect of TSA, gonadotropin-releasing hormone stimulated RALDH expression in LβT2 cells. RA directly applied to the pituitary cells stimulated the transcriptional activity of the FSHβ promoter. In addition, α- and LHβ-subunit promoters were also activated by RA. Our results suggest that TSA specifically increases FSHβ expression with a concomitant increase in RALDHs; however, RALDH and RA are not directly involved in the specific regulation of FSHβ by TSA.
authors.affiliation: Department of Obstetrics and Gynecology, Shimane University School of Medicine, Izumo 693-8501, Japan.
authors.forename: Tselmeg
authors.initials: T
authors.lastname: Mijiddorj
authors.affiliation: Department of Obstetrics and Gynecology, Shimane University School of Medicine, Izumo 693-8501, Japan. Electronic address: kanasaki@med.shimane-u.ac.jp.
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Kanasaki
authors.affiliation: Department of Obstetrics and Gynecology, Shimane University School of Medicine, Izumo 693-8501, Japan.
authors.forename: Unurjargal
authors.initials: U
authors.lastname: Sukhbaatar
authors.affiliation: Department of Obstetrics and Gynecology, Shimane University School of Medicine, Izumo 693-8501, Japan.
authors.forename: Aki
authors.initials: A
authors.lastname: Oride
authors.affiliation: Department of Obstetrics and Gynecology, Shimane University School of Medicine, Izumo 693-8501, Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Ishihara
authors.affiliation: Department of Obstetrics and Gynecology, Shimane University School of Medicine, Izumo 693-8501, Japan.
authors.forename: Satoru
authors.initials: S
authors.lastname: Kyo
mesh_terms: D000107:Acetylation; D000818:Animals; D005640:Follicle Stimulating Hormone / Q000378:metabolism; D037201:Follicle Stimulating Hormone, beta Subunit / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000502:physiology*; D052681:Gonadotrophs / Q000378:metabolism; D007987:Gonadotropin-Releasing Hormone / Q000378:metabolism; D056572:Histone Deacetylase Inhibitors; D006655:Histone Deacetylases / Q000378:metabolism*; D006877:Hydroxamic Acids / Q000494:pharmacology*; D037101:Luteinizing Hormone, beta Subunit / Q000378:metabolism; D010902:Pituitary Gland / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000187:drug effects; D011499:Protein Processing, Post-Translational; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D050697:Retinal Dehydrogenase / Q000235:genetics / Q000378:metabolism*; D015533:Transcriptional Activation / Q000187:drug effects; D014212:Tretinoin / Q000378:metabolism*
chemical_list: D037201:Follicle Stimulating Hormone, beta Subunit; D056572:Histone Deacetylase Inhibitors; D006877:Hydroxamic Acids; D037101:Luteinizing Hormone, beta Subunit; D012333:RNA, Messenger; D007987:Gonadotropin-Releasing Hormone; C012589:trichostatin A; D014212:Tretinoin; D005640:Follicle Stimulating Hormone; D050697:Retinal Dehydrogenase; D006655:Histone Deacetylases
keywords: Follicle-stimulating hormone; Retinaldehyde dehydrogenase; Retinoic acid; Trichostatin A