title: Adipose-Derived Stromal Vascular Fraction Cell Effects on a Rodent Model of Thin Endometrium.
abstract: Endometrial dysfunction affects approximately 1% of infertile women, and there is currently no standard therapy for improving fertility treatment outcomes in these patients. In our study, we utilized a rodent model of thin endometrium to test whether intrauterine application of adipose-derived stromal vascular fraction cells (SVF) could improve morphological and physiological markers of endometrial receptivity. Using anhydrous ethanol, endometrial area and gland density were significantly reduced in our model of thin endometrium. Application of SVF was associated with a 29% reduction in endometrial vascular endothelial growth factor (VEGF) expression and significant increases in uterine artery systolic/diastolic velocity ratios and resistance index values, suggesting reduced diastolic microvascular tone. However, no significant improvements in endometrial area or gland density were observed following SVF treatment. 3D confocal imaging demonstrated poor engraftment of SVF cells into recipient tissue, which likely contributed to the negative results of this study. We suspect modified treatment protocols utilizing adjuvant estrogen and/or tail vein cell delivery may improve SVF retention and therapeutic response in subsequent studies. SVF is an easily-obtainable cell product with regenerative capability that may have a future role in the treatment of infertile women with endometrial dysfunction.
authors.affiliation: Department of Obstetrics, Gynecology and Women's Health, Division of Reproductive Endocrinology and Infertility, University of Louisville School of Medicine, Louisville, Kentucky, United States of America.
authors.forename: Robert K
authors.initials: RK
authors.lastname: Hunter
authors.affiliation: Department of Biochemistry and Molecular Genetics, University of Louisville School of Medicine, Louisville, Kentucky, United States of America.
authors.forename: Chris D
authors.initials: CD
authors.lastname: Nevitt
authors.affiliation: Department of Bioinformatics and Biostatistics, University of Louisville School of Public Health and Information Sciences, Louisville, Kentucky, United States of America.
authors.forename: Jeremy T
authors.initials: JT
authors.lastname: Gaskins
authors.affiliation: Cardiovascular Innovation Institute, Louisville, Kentucky, United States of America.
authors.forename: Bradley B
authors.initials: BB
authors.lastname: Keller
authors.affiliation: Department of Obstetrics, Gynecology and Women's Health, Division of Reproductive Endocrinology and Infertility, University of Louisville School of Medicine, Louisville, Kentucky, United States of America.
authors.forename: Henry C L
authors.initials: HC
authors.lastname: Bohler
authors.affiliation: Cardiovascular Innovation Institute, Louisville, Kentucky, United States of America.|Department of Physiology, University of Louisville School of Medicine, Louisville, Kentucky, United States of America.
authors.forename: Amanda J
authors.initials: AJ
authors.lastname: LeBlanc
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000378:metabolism; D000273:Adipose Tissue / Q000166:cytology* / Q000378:metabolism; D050154:Adiposity / Q000502:physiology; D053687:Adult Stem Cells / Q000166:cytology; D000818:Animals; D002478:Cells, Cultured; D004717:Endometrium / Q000166:cytology* / Q000378:metabolism; D005260:Female; D007247:Infertility, Female / Q000473:pathology / Q000628:therapy; D023421:Models, Animal; D051381:Rats; D017207:Rats, Sprague-Dawley; D017154:Stromal Cells / Q000166:cytology* / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D042461:Vascular Endothelial Growth Factor A