title: Gag-Specific CD4 and CD8 T-Cell Proliferation in Adolescents and Young Adults with Perinatally Acquired HIV-1 Infection Is Associated with Ethnicity - The ANRS-EP38-IMMIP Study.
abstract: The ANRS-EP38-IMMIP study aimed to provide a detailed assessment of the immune status of perinatally infected youths living in France. We studied Gag-specific CD4 and CD8 T-cell proliferation and the association between the proliferation of these cells, demographic factors and HIV disease history. We included 93 youths aged between 15 and 24 years who had been perinatally infected with HIV. Sixty-nine had undergone valid CFSE-based T-cell proliferation assays. Gag-specific proliferation of CD4 and CD8 T cells was detected in 12 (16%) and 30 (38%) patients, respectively. The Gag-specific proliferation of CD4 and CD8 T cells was more frequently observed in black patients than in patients from other ethnic groups (CD4: 32% vs. 4%, P = 0.001; CD8: 55% vs. 26%, P = 0.02). Among aviremic patients, the duration of viral suppression was shorter in CD8 responders than in CD8 nonresponders (medians: 54 vs. 20 months, P = 0.04). Among viremic patients, CD8 responders had significantly lower plasma HIV RNA levels than CD8 nonresponders (2.7 vs. 3.7 log10 HIV-RNA copies/ml, P = 0.02). In multivariate analyses including sex and HIV-1 subtype as covariables, Gag-specific CD4 T-cell proliferation was associated only with ethnicity, whereas Gag-specific CD8 T-cell proliferation was associated with both ethnicity and the duration of viral suppression. Both CD4 and CD8 responders reached their nadir CD4 T-cell percentages at younger ages than their nonresponder counterparts (6 vs. 8 years, P = 0.04 for both CD4 and CD8 T-cell proliferation). However, these associations were not significant in multivariate analysis. In conclusion, after at least 15 years of HIV infection, Gag-specific T-cell proliferation was found to be more frequent in black youths than in patients of other ethnic groups, despite all the patients being born in the same country, with similar access to care.
authors.affiliation: CESP INSERM U1018, Le Kremlin-Bicêtre, France.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Le Chenadec
authors.affiliation: Institut Pasteur, Unité de Régulation des Infections Rétrovirales, Paris, France.
authors.forename: Daniel
authors.initials: D
authors.lastname: Scott-Algara
authors.affiliation: AP-HP, Unité Immunologie et Hématologie Pédiatrique, Hôpital Necker-Enfants Malades, Paris, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Blanche
authors.affiliation: Institut Pasteur, Unité de Régulation des Infections Rétrovirales, Paris, France.
authors.forename: Céline
authors.initials: C
authors.lastname: Didier
authors.affiliation: Institut Pasteur, Unité d'Epidémiologie et Physiopathologie des Virus Oncogènes, Paris, France.|CNRS, UMR 3569, Paris, France.
authors.forename: Thomas
authors.initials: T
authors.lastname: Montange
authors.affiliation: EA7327, Université Paris Descartes, Sorbonne Paris Cité, Faculté de Médecine, Paris, France.|AP-HP, Centre de Diagnostic et de Thérapeutique, Hôpital de l'Hôtel-Dieu, Paris, France.
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Viard
authors.affiliation: AP-HP, Service d'Hématologie et d'Oncologie Pédiatrique, Hôpital Trousseau, Paris, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Dollfus
authors.affiliation: EA7327, Université Paris Descartes, Sorbonne Paris Cité, Faculté de Médecine, Paris, France.|AP-HP, Laboratoire de Virologie, Hôpital Necker-Enfants Malades, Paris, France.
authors.forename: Véronique
authors.initials: V
authors.lastname: Avettand-Fenoel
authors.affiliation: EA7327, Université Paris Descartes, Sorbonne Paris Cité, Faculté de Médecine, Paris, France.|AP-HP, Laboratoire de Virologie, Hôpital Necker-Enfants Malades, Paris, France.
authors.forename: Christine
authors.initials: C
authors.lastname: Rouzioux
authors.affiliation: CESP INSERM U1018, Le Kremlin-Bicêtre, France.|Université Paris-Sud, Le Kremlin-Bicêtre, France.
authors.forename: Josiane
authors.initials: J
authors.lastname: Warszawski
authors.affiliation: Institut Pasteur, Unité d'Epidémiologie et Physiopathologie des Virus Oncogènes, Paris, France.|CNRS, UMR 3569, Paris, France.
authors.forename: Florence
authors.initials: F
authors.lastname: Buseyne
mesh_terms: D000293:Adolescent; D000328:Adult; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D005006:Ethnicity / Q000706:statistics & numerical data*; D005260:Female; D005434:Flow Cytometry; D015658:HIV Infections / Q000208:ethnology* / Q000276:immunology / Q000821:virology; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D008213:Lymphocyte Activation / Q000276:immunology*; D008297:Male; D018743:Perinatal Care; D010802:Phylogeny; D060888:Real-Time Polymerase Chain Reaction; D055815:Young Adult; D054301:gag Gene Products, Human Immunodeficiency Virus / Q000276:immunology*
chemical_list: D054301:gag Gene Products, Human Immunodeficiency Virus