title: Effect of α-Tocopherol on the Microscopic Dynamics of Dimyristoylphosphatidylcholine Membrane.
abstract: Vitamin E behaves as an antioxidant and is well known for its protective properties of the lipid membrane. The most biologically active form of vitamin E in the human organism is α-tocopherol (aToc). Very recently (Marquardt, D.; et al. J. Am. Chem. Soc. 2014, 136, 203-210) it has been shown that aToc resides near the center of dimyristoylphosphatidylcholine (DMPC) bilayer, which is in stark contrast with other PC membranes, where aToc is located near the lipid-water interface. Here we report an unusual effect of this exceptional location of aToc on the dynamical behavior of DMPC membrane probed by incoherent elastic and quasielastic neutron scattering. For pure DMPC vesicles, elastic scan data show two step-like drops in the elastic intensity at 288 and 297 K, which correspond to the pre- and main phase transitions, respectively. However, inclusion of aToc into DMPC membrane inhibits the step-like elastic intensity drops, indicating a significant impact of aToc on the phase behavior of the membrane. This observation is supported by our differential scanning calorimetry data, which shows that inclusion of aToc leads to a significant broadening of the main phase transition peak, whereas the peak corresponding to the pretransition disappears. We have performed quasielastic neutron scattering (QENS) measurements on DMPC vesicles with various concentrations of aToc at 280, 293, and 310 K. We have found that aToc affects both the lateral diffusion and the internal motions of the lipid molecules. Below the main phase transition temperature inclusion of aToc accelerates both the lateral and the internal lipid motions. On the other hand, above the main phase transition temperature the addition of aToc restricts only the internal motion, without a significant influence on the lateral motion. Our results support the finding that the location of aToc in DMPC membrane is deep within the bilayer.
authors.affiliation: Biology and Soft Matter Division, Neutron Sciences Directorate, Oak Ridge National Laboratory , Oak Ridge, Tennessee 37831, United States.|Solid State Physics Division, Bhabha Atomic Research Centre , Mumbai 400085, India.
authors.forename: V K
authors.initials: VK
authors.lastname: Sharma
authors.affiliation: Chemical and Engineering Materials Division, Neutron Sciences Directorate, Oak Ridge National Laboratory , Oak Ridge, Tennessee 37831, United States.
authors.forename: E
authors.initials: E
authors.lastname: Mamontov
authors.affiliation: National Institute of Standards and Technology Center for Neutron Research , Gaithersburg, Maryland 20899, United States.|Department of Materials Science and Engineering, University of Maryland , College Park, Maryland 20742, United States.
authors.forename: M
authors.initials: M
authors.lastname: Tyagi
authors.affiliation: Biology and Soft Matter Division, Neutron Sciences Directorate, Oak Ridge National Laboratory , Oak Ridge, Tennessee 37831, United States.
authors.forename: V S
authors.initials: VS
authors.lastname: Urban
mesh_terms: D002151:Calorimetry; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry*; D006801:Humans; D008051:Lipid Bilayers / Q000737:chemistry*; D015394:Molecular Structure; D009502:Neutrons; D012542:Scattering, Radiation; D013816:Thermodynamics; D024502:alpha-Tocopherol / Q000737:chemistry*
chemical_list: D008051:Lipid Bilayers; D024502:alpha-Tocopherol; D004134:Dimyristoylphosphatidylcholine