title: The Entamoeba histolytica, Arp2/3 Complex Is Recruited to Phagocytic Cups through an Atypical Kinase EhAK1.
abstract: The parasite Entamoeba histolytica is the etiological agent of amoebiasis and phagocytosis plays a key role in virulence of this organism. Signaling pathways involved in activation of cytoskeletal dynamics required for phagocytosis remain to be elucidated. Phagocytosis is initiated with sequential recruitment of EhC2PK, EhCaBP1, EhCaBP3 and an atypical kinase EhAK1 after particle attachment. Here we show that EhARPC1, an essential subunit of the actin branching complex Arp 2/3 is recruited to the phagocytic initiation sites by EhAK1. Imaging, expression knockdown of different molecules and pull down experiments suggest that EhARPC1 interacts with EhAK1 and that it is required during initiation of phagocytosis and phagosome formation. Moreover, recruitment of EhARPC2 at the phagocytosis initiation by EhAK1 is also observed, indicating that the Arp 2/3 complex is recruited. In conclusion, these results suggests a novel mechanism of recruitment of Arp 2/3 complex during phagocytosis in E. histolytica.
authors.affiliation: School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.
authors.forename: Mrigya
authors.initials: M
authors.lastname: Babuta
authors.affiliation: School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.
authors.forename: M Shahid
authors.initials: MS
authors.lastname: Mansuri
authors.affiliation: School of Environmental Sciences, Jawaharlal Nehru University, New Delhi, India.
authors.forename: Sudha
authors.initials: S
authors.lastname: Bhattacharya
authors.affiliation: School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.|School of Natural Sciences, Department of life Sciences, Shiv Nadar University, Uttar Pradesh, India.
authors.forename: Alok
authors.initials: A
authors.lastname: Bhattacharya
mesh_terms: D051376:Actin-Related Protein 2-3 Complex / Q000378:metabolism*; D000199:Actins / Q000378:metabolism; D000818:Animals; D015153:Blotting, Western; D003599:Cytoskeleton / Q000378:metabolism; D004748:Entamoeba histolytica / Q000378:metabolism*; D004749:Entamoebiasis / Q000378:metabolism*; D004912:Erythrocytes / Q000469:parasitology; D005455:Fluorescent Antibody Technique; D047468:Immunoprecipitation; D051379:Mice; D010587:Phagocytosis / Q000502:physiology*; D016133:Polymerase Chain Reaction; D011494:Protein Kinases / Q000378:metabolism*; D015800:Protozoan Proteins / Q000378:metabolism*; D011817:Rabbits; D015398:Signal Transduction / Q000502:physiology
chemical_list: D051376:Actin-Related Protein 2-3 Complex; D000199:Actins; D015800:Protozoan Proteins; D011494:Protein Kinases