title: [Changes of six serum biomarkers of rabbits with experimental osteoarthritis].
abstract: OBJECTIVE
  To investigate the value and significance of six serum biomarkers to the early diagnosis of osteoarthritis by means of quantitative detection of interleukin-1 beta (IL-1β), matrix metalloproteinase 13 (MMP-13), IL-6, C-terminal telopeptide of collagen type II (CTX2), cyclooxygenase 2 (COX2) and prostaglandin E2 (PGE2) levels with time going on.
  
  
  METHODS
  40 New Zealand white rabbits were divided into experimental group (n=20) and control group (n=20). Injection of collagenase into articular cavity was performed in the experimental group for modeling. No treatment was done in the control group. Pathological observation, X-ray examination and HE staining of knee joints were conducted to verify the establishment of modeling. IL-1, MMP-13, IL-6, CTX2, COX2 and PGE2 levels were detected every two weeks after modeling in the experimental group, and these biomarkers were also detected in the control group at the same time points.
  
  
  RESULTS
  Levels of the six serum biomarkers in the experimental group were significantly higher than those in the control group at each time point after modeling and were the most distinguishing at the 4th week.
  
  
  CONCLUSIONS
  Combined detection of IL-1β, IL-6, CTX2, COX2 and PGE2 in serum is helpful for early diagnosis of osteoarthritis.
authors.affiliation: Faculty of Laboratory Medicine, Xiangya Medical College, Central South University, Changsha 410013, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Shi
authors.affiliation: Faculty of Laboratory Medicine, Xiangya Medical College, Central South University, Changsha 410013, China. *Corresponding author, E-mail: ggboxy@163.com.
authors.forename: Ge
authors.initials: G
authors.lastname: Gao
authors.affiliation: Faculty of Laboratory Medicine, Xiangya Medical College, Central South University, Changsha 410013, China.
authors.forename: Zuwei
authors.initials: Z
authors.lastname: Yan
authors.affiliation: Faculty of Laboratory Medicine, Xiangya Medical College, Central South University, Changsha 410013, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Wei
authors.affiliation: Department of Rheumatology and Immunology, Second Xiangya Hospital, Central South University, Changsha 410011, China.
authors.forename: Zibo
authors.initials: Z
authors.lastname: Gao
authors.affiliation: Department of Rheumatology and Immunology, Second Xiangya Hospital, Central South University, Changsha 410011, China.
authors.forename: Jiesheng
authors.initials: J
authors.lastname: Gao
authors.affiliation: Department of Orthopedics, Third Xiangya Hospital, Central South University, Changsha 410013, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Department of Obstetrics and Gynecology, Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Hu
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood*; D024043:Collagen Type II / Q000097:blood; D051546:Cyclooxygenase 2 / Q000097:blood; D015232:Dinoprostone / Q000097:blood; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D053583:Interleukin-1beta / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D008297:Male; D053509:Matrix Metalloproteinase 13 / Q000097:blood; D020370:Osteoarthritis, Knee / Q000097:blood* / Q000175:diagnosis; D011817:Rabbits
chemical_list: D015415:Biomarkers; D024043:Collagen Type II; D053583:Interleukin-1beta; D015850:Interleukin-6; D051546:Cyclooxygenase 2; D053509:Matrix Metalloproteinase 13; D015232:Dinoprostone