title: Influence of immobilization strategies on biosensing response characteristics: A comparative study.
abstract: The immobilization technique plays an important role in fabrication of a biosensor. NiO based cholesterol biosensor has been used to study the effect of various immobilization techniques on the biosensing response characteristics. The biosensors were fabricated by immobilizing cholesterol oxidase on NiO thin films by three different immobilization techniques viz. physisorption, cross-linking and covalent binding. The study reveals a strong dependence of biosensing response on corresponding immobilization technique. The biosensor based on immobilization by covalent bonding shows superior response characteristics as compared to others owing to its zero length. The results highlight the significance of immobilization technique for biosensor fabrication.
authors.affiliation: Department of Physics & Astrophysics, University of Delhi, Delhi-7, India.
authors.forename: Gurpreet
authors.initials: G
authors.lastname: Kaur
authors.affiliation: Department of Physics, Dyal Singh College, University of Delhi, Lodi Road, Delhi-3, India.
authors.forename: Shibu
authors.initials: S
authors.lastname: Saha
authors.affiliation: Department of Physics, Miranda House, University of Delhi, Delhi-7, India.
authors.forename: Monika
authors.initials: M
authors.lastname: Tomar
authors.affiliation: Department of Physics & Astrophysics, University of Delhi, Delhi-7, India. Electronic address: vgupta@physics.du.ac.in.
authors.forename: Vinay
authors.initials: V
authors.lastname: Gupta
mesh_terms: D000327:Adsorption; D015374:Biosensing Techniques / Q000379:methods*; D002789:Cholesterol Oxidase / Q000378:metabolism*; D003432:Cross-Linking Reagents; D004800:Enzymes, Immobilized; D007700:Kinetics; D009532:Nickel / Q000378:metabolism; D010783:Photometry; D011485:Protein Binding; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D003432:Cross-Linking Reagents; D004800:Enzymes, Immobilized; D009532:Nickel; C028007:nickel monoxide; D002789:Cholesterol Oxidase
keywords: Biosensor; Covalent immobilization; Crosslinking; Immobilization technique; Physisorption