title: Mitochondrial oxidative phosphorylation complexes exist in the sarcolemma of skeletal muscle.
abstract: Although proteomic analyses have revealed the presence of mitochondrial oxidative phosphorylation (OXPHOS) proteins in the plasma membrane, there have been no in-depth evaluations of the presence or function of OXPHOS I-V in the plasma membrane. Here, we demonstrate the in situ localization of OXPHOS I-V complexes to the sarcolemma of skeletal muscle by immunofluorescence and immunohistochemistry. A portion of the OXPHOS I-V complex proteins was not co-stained with MitoTracker but co-localized with caveolin-3 in the sarcolemma of mouse gastrocnemius. Mitochondrial matrix-facing OXPHOS complex subunits were ectopically expressed in the sarcolemma of the non-permeabilized muscle fibers and C2C12 myotubes. The sarcolemmal localization of cytochrome c was also observed from mouse gastrocnemius muscles and C2C12 myotubes, as determined by confocal and total internal resonance fluorescence (TIRF) microscopy. Based on these data, we conclude that a portion of OXPHOS complexes is localized in the sarcolemma of skeletal muscle and may have non-canonical functions. [BMB Reports 2016; 49(2): 116-121].
authors.affiliation: Division of Life Sciences, Korea University, Seoul 02841, Korea.
authors.forename: Hyun
authors.initials: H
authors.lastname: Lee
authors.affiliation: Division of Life Sciences, Korea University, Seoul 02841, Korea.
authors.forename: Seung-Hyeob
authors.initials: SH
authors.lastname: Kim
authors.affiliation: Department of Biomedical Sciences, College of Medicine, Inha University, Incheon 22212, Korea.
authors.forename: Jae-Seon
authors.initials: JS
authors.lastname: Lee
authors.affiliation: Department of Chemistry, Seoul National University, Seoul 08826, Korea.
authors.forename: Yun-Hee
authors.initials: YH
authors.lastname: Yang
authors.affiliation: Department of Chemistry, Seoul National University, Seoul 08826, Korea.
authors.forename: Jwa-Min
authors.initials: JM
authors.lastname: Nam
authors.affiliation: Division of Life Sciences, Korea University, Seoul 02841, Korea.
authors.forename: Bong-Woo
authors.initials: BW
authors.lastname: Kim
authors.affiliation: Division of Life Sciences, Korea University, Seoul 02841, Korea.
authors.forename: Young-Gyu
authors.initials: YG
authors.lastname: Ko
mesh_terms: D000818:Animals; D051244:Caveolin 3 / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D019069:Cell Respiration; D045304:Cytochromes c / Q000378:metabolism; D005110:Extracellular Space / Q000378:metabolism; D008297:Male; D008810:Mice, Inbred C57BL; D008928:Mitochondria / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000378:metabolism*; D009243:NAD / Q000378:metabolism; D010085:Oxidative Phosphorylation; D010101:Oxygen Consumption; D012508:Sarcolemma / Q000378:metabolism*
chemical_list: D051244:Caveolin 3; D009243:NAD; D045304:Cytochromes c