title: Development and Validation of a PCR Assay To Detect the Prairie Epidemic Strain of Pseudomonas aeruginosa from Patients with Cystic Fibrosis.
abstract: The monitoring of epidemic Pseudomonas aeruginosa is important for cystic fibrosis (CF) infection control. The prairie epidemic strain (PES) is common in western Canadian CF clinics. Using whole-genome sequencing, we identified a novel genomic island and developed a PCR assay for PES. Against a collection of 186 P. aeruginosa isolates, the assay had 98% sensitivity and 100% specificity.
authors.affiliation: Department of Veterinary Medicine, University of Calgary, Calgary, Canada.
authors.forename: M
authors.initials: M
authors.lastname: Workentine
authors.affiliation: Department of Microbiology, Immunology, and Infectious Diseases, University of Calgary, Calgary, Canada.
authors.forename: A
authors.initials: A
authors.lastname: Poonja
authors.affiliation: Department of Microbiology, Immunology, and Infectious Diseases, University of Calgary, Calgary, Canada.
authors.forename: B
authors.initials: B
authors.lastname: Waddell
authors.affiliation: Department of Biological Sciences, University of Calgary, Calgary, Canada.
authors.forename: J
authors.initials: J
authors.lastname: Duong
authors.affiliation: Department of Microbiology, Immunology, and Infectious Diseases, University of Calgary, Calgary, Canada Department of Biological Sciences, University of Calgary, Calgary, Canada.
authors.forename: D G
authors.initials: DG
authors.lastname: Storey
authors.affiliation: Department of Microbiology, Immunology, and Infectious Diseases, University of Calgary, Calgary, Canada Department of Medicine, University of Calgary, Calgary, Canada.
authors.forename: D
authors.initials: D
authors.lastname: Gregson
authors.affiliation: Department of Microbiology, Immunology, and Infectious Diseases, University of Calgary, Calgary, Canada Department of Medicine, University of Calgary, Calgary, Canada.
authors.forename: R
authors.initials: R
authors.lastname: Somayaji
authors.affiliation: Department of Microbiology, Immunology, and Infectious Diseases, University of Calgary, Calgary, Canada Department of Medicine, University of Calgary, Calgary, Canada.
authors.forename: H R
authors.initials: HR
authors.lastname: Rabin
authors.affiliation: Department of Microbiology, Immunology, and Infectious Diseases, University of Calgary, Calgary, Canada Department of Medicine, McMaster University, Hamilton, Ontario, Canada Department of Biochemistry, McMaster University, Hamilton, Ontario, Canada The Farncombe Family Digestive Health Research Institute, McMaster University, Hamilton, Ontario, Canada.
authors.forename: M G
authors.initials: MG
authors.lastname: Surette
authors.affiliation: Department of Microbiology, Immunology, and Infectious Diseases, University of Calgary, Calgary, Canada Department of Medicine, University of Calgary, Calgary, Canada mdparkin@ucalgary.ca.
authors.forename: M D
authors.initials: MD
authors.lastname: Parkins
mesh_terms: D003550:Cystic Fibrosis / Q000150:complications*; D016521:Electrophoresis, Gel, Pulsed-Field; D016680:Genome, Bacterial; D006801:Humans; D058885:Multilocus Sequence Typing / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods; D011552:Pseudomonas Infections / Q000175:diagnosis* / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000145:classification* / Q000235:genetics*; D019105:Random Amplified Polymorphic DNA Technique; D012680:Sensitivity and Specificity