title: Simultaneous Raising of Rabbit Monoclonal Antibodies to Fluoroquinolones with Diverse Recognition Functionalities via Single Mixture Immunization.
abstract: Highly specific monoclonal and polyclonal antibodies are the key components in a diverse set of immunoassay applications, from research work to routine monitoring and analysis. In the current manuscript, combinatorial strategies for a single mixture immunization, screening and rabbit hybridoma cell technology were described. Fluoroquinolones (FQs) drugs were chosen as representative analytes. Six FQs were conjugated with bovine serum albumin and used as immunogens for subsequent immunization, while a mixture of all was injected for coimmunization. The hybridomas obtained against the individual and multiple FQs were used for the production of diverse varieties of rabbit monoclonal antibodies (RabMAbs) against the target analytes. As was proven by indirect competitive ELISA and quantitative lateral flow immunoassay, this approach opens a new way for simultaneously obtaining functional monoclonal antibodies which are capable of recognizing both individual and multiple analytes in a single preparation circle. This addresses various needs of different monitoring regulations as analytical methodology advances.
authors.affiliation: Key Laboratory of Food Safety Research, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences , 294 Taiyuan Road, Shanghai 200031, P. R. China ;|Key Laboratory of Food Safety Risk Assessment, Ministry of Health , Beijing, 100021, China.|School of Biosystems Engineering and Food Science, Zhejiang University , 388 Yuhangtang Road, Hangzhou 310058, P. R. China.
authors.forename: Na
authors.initials: N
authors.lastname: Liu
authors.affiliation: Key Laboratory of Food Safety Research, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences , 294 Taiyuan Road, Shanghai 200031, P. R. China ;|Key Laboratory of Food Safety Risk Assessment, Ministry of Health , Beijing, 100021, China.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Food Safety Research, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences , 294 Taiyuan Road, Shanghai 200031, P. R. China ;|Key Laboratory of Food Safety Risk Assessment, Ministry of Health , Beijing, 100021, China.
authors.forename: Yanglan
authors.initials: Y
authors.lastname: Tan
authors.affiliation: School of Biosystems Engineering and Food Science, Zhejiang University , 388 Yuhangtang Road, Hangzhou 310058, P. R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Lu
authors.affiliation: School of Biosystems Engineering and Food Science, Zhejiang University , 388 Yuhangtang Road, Hangzhou 310058, P. R. China.
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
authors.affiliation: College of Plant Science and Technology, Huazhong Agricultural University , No. 1 Shizishan Street, Hongshan District, Wuhan, Hubei 430070, PR China.
authors.forename: Yucai
authors.initials: Y
authors.lastname: Liao
authors.affiliation: Laboratory of Food Analysis, Faculty of Pharmaceutical Sciences, Ghent University , Ottergemsesteenweg 460, 9000 Ghent, Belgium.
authors.forename: Natalia
authors.initials: N
authors.lastname: Beloglazova
authors.affiliation: Laboratory of Food Analysis, Faculty of Pharmaceutical Sciences, Ghent University , Ottergemsesteenweg 460, 9000 Ghent, Belgium.
authors.forename: Sarah
authors.initials: S
authors.lastname: De Saeger
authors.affiliation: School of Biosystems Engineering and Food Science, Zhejiang University , 388 Yuhangtang Road, Hangzhou 310058, P. R. China.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Zheng
authors.affiliation: Key Laboratory of Food Safety Research, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences , 294 Taiyuan Road, Shanghai 200031, P. R. China ;|Key Laboratory of Food Safety Risk Assessment, Ministry of Health , Beijing, 100021, China.
authors.forename: Aibo
authors.initials: A
authors.lastname: Wu
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D000937:Antigen-Antibody Reactions; D002417:Cattle; D024841:Fluoroquinolones / Q000737:chemistry / Q000276:immunology*; D006825:Hybridomas / Q000276:immunology* / Q000378:metabolism; D007114:Immunization / Q000379:methods*; D007118:Immunoassay / Q000379:methods; D011817:Rabbits; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D024841:Fluoroquinolones; D012710:Serum Albumin, Bovine