title: Cloning and Functional Characterization of Two BTB Genes in the Predatory Mite Metaseiulus occidentalis.
abstract: Proteins containing the BTB (Bric-à-brac, tramtrack, and Broad Complex) domain typically share low sequence similarities and are involved in a wide range of cellular functions. We previously identified two putative and closely related BTB genes, BTB1 and BTB2, in the genome of the predatory mite Metaseiulus occidentalis. In the current study, full-length BTB1 and BTB2 cDNAs were cloned and sequenced. BTB1 and BTB2 encode proteins of 380 and 401 amino acids, respectively. BTB1 and BTB2 proteins each contain an N-terminal BTB domain and no other identifiable domains. Thus, they belong to a large category of BTB-domain proteins that are widely distributed in eukaryotes, yet with largely unknown function(s). BTB1 and BTB2 gene knockdowns in M. occidentalis females using RNAi reduced their fecundity by approximately 40% and 73%, respectively, whereas knockdown had no impact on their survival or the development of their offspring. These findings suggest these two proteins may be involved in processes related to egg production in this predatory mite, expanding the list of functions attributed to these diverse proteins.
authors.affiliation: Department of Entomology and Nematology, PO Box 11620, University of Florida, Gainesville, Florida, 32611, United States of America.
authors.forename: Ke
authors.initials: K
authors.lastname: Wu
authors.affiliation: Department of Entomology and Nematology, PO Box 11620, University of Florida, Gainesville, Florida, 32611, United States of America.
authors.forename: Marjorie A
authors.initials: MA
authors.lastname: Hoy
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D060829:Arthropod Proteins / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D005298:Fertility / Q000235:genetics; D008925:Mites / Q000235:genetics*; D017434:Protein Structure, Tertiary; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D060829:Arthropod Proteins; D012333:RNA, Messenger; D034741:RNA, Small Interfering