title: Suppressive effects of dRYamides on feeding behavior of the blowfly, Phormia regina.
abstract: Recently, dRYamides-1 and -2 have been identified as ligands of the neuropeptide Y-like receptor CG5811 in Drosophila melanogaster. It has also been reported in brief that injection of dRYamide-1suppresses the early feeding behavior called proboscis extension reflex (PER) in the blowfly Phormia regina. Immunohistochemical analyses by our group using anti-dRYamide-1 antiserum indicated symmetrical localization of 32 immunoreactive cells in the brain of P. regina. In order to analyze the mechanism of feeding regulation, we further investigated the effects of dRYamide-1 and -2 on intake volume, PER exhibition, and activity of the sugar receptor neuron. After injection of dRYamide-1 or -2, flies showed little change in the intake volume of sucrose solution, but a significant depression of PER to sucrose. Injection of dRYamide-1 revealed a significant decrease in the responsiveness of the sugar receptor neuron, although the injection of dRYamide-2 did not. These results suggest that the dRYamide peptides decrease feeding motivation in flies, as evaluated by PER threshold, through a mechanism that partially involves desensitization of the sugar receptor neuron.
authors.affiliation: Department of Biology, Graduate School of Science, Kobe University, Nada, Kobe, 657-8501 Japan.
authors.forename: Toru
authors.initials: T
authors.lastname: Maeda
authors.affiliation: Department of Biology, Graduate School of Science, Kobe University, Nada, Kobe, 657-8501 Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Nakamura
authors.affiliation: Department of Biology, Graduate School of Science, Kobe University, Nada, Kobe, 657-8501 Japan.
authors.forename: Hajime
authors.initials: H
authors.lastname: Shiotani
authors.affiliation: Department of Biology, Graduate School of Science, Kobe University, Nada, Kobe, 657-8501 Japan.
authors.forename: Masaru K
authors.initials: MK
authors.lastname: Hojo
authors.affiliation: Department of Biology, Graduate School of Natural Science and Technology, Okayama University, Okayama, 700-8530 Japan.
authors.forename: Taishi
authors.initials: T
authors.lastname: Yoshii
authors.affiliation: Interdisciplinary Research Organization, University of Miyazaki, Miyazaki, 889-2192 Japan.
authors.forename: Takanori
authors.initials: T
authors.lastname: Ida
authors.affiliation: Molecular Genetics, Institute of Life Sciences, Kurume University, Fukuoka, 839-0864 Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Sato
authors.affiliation: Department of Biochemistry, National Cerebral and Cardiovascular Center Research Institute, Suita, 565-0873 Japan.
authors.forename: Morikatsu
authors.initials: M
authors.lastname: Yoshida
authors.affiliation: Department of Biochemistry, National Cerebral and Cardiovascular Center Research Institute, Suita, 565-0873 Japan.
authors.forename: Mikiya
authors.initials: M
authors.lastname: Miyazato
authors.affiliation: Molecular Genetics, Institute of Life Sciences, Kurume University, Fukuoka, 839-0864 Japan.
authors.forename: Masayasu
authors.initials: M
authors.lastname: Kojima
authors.affiliation: Department of Biology, Graduate School of Science, Kobe University, Nada, Kobe, 657-8501 Japan.
authors.forename: Mamiko
authors.initials: M
authors.lastname: Ozaki
keywords: Bioactive peptide; Feeding regulation; Fly; Taste response; dRYamide