title: Axon-First Neuritogenesis on Vertical Nanowires.
abstract: In this work, we report that high-density, vertically grown silicon nanowires (vg-SiNWs) direct a new in vitro developmental pathway of primary hippocampal neurons. Neurons on vg-SiNWs formed a single, extremely elongated major neurite earlier than minor neurites, which led to accelerated polarization. Additionally, the development of lamellipodia, which generally occurs on 2D culture coverslips, was absent on vg-SiNWs. The results indicate that surface topography is an important factor that influences neuronal development and also provide implications for the role of topography in neuronal development in vivo.
authors.affiliation: Center for Cell-Encapsulation Research and Molecular-Level Interface Research Center, Department of Chemistry, KAIST , Daejeon 34141, Korea.|Department of Bio and Brain Engineering, KAIST , Daejeon 34141, Korea.
authors.forename: Kyungtae
authors.initials: K
authors.lastname: Kang
authors.affiliation: Department of Chemistry, Sookmyung Women's University , Seoul 04310, Korea.
authors.forename: Yi-Seul
authors.initials: YS
authors.lastname: Park
authors.affiliation: Center for Cell-Encapsulation Research and Molecular-Level Interface Research Center, Department of Chemistry, KAIST , Daejeon 34141, Korea.
authors.forename: Matthew
authors.initials: M
authors.lastname: Park
authors.affiliation: Department of Bio and Brain Engineering, KAIST , Daejeon 34141, Korea.
authors.forename: Min Jee
authors.initials: MJ
authors.lastname: Jang
authors.affiliation: School of Materials Science and Engineering, Gwangju Institute of Science and Technology , Gwangju 61005, Korea.
authors.forename: Seong-Min
authors.initials: SM
authors.lastname: Kim
authors.affiliation: Center for Cell-Encapsulation Research and Molecular-Level Interface Research Center, Department of Chemistry, KAIST , Daejeon 34141, Korea.
authors.forename: Juno
authors.initials: J
authors.lastname: Lee
authors.affiliation: Center for Cell-Encapsulation Research and Molecular-Level Interface Research Center, Department of Chemistry, KAIST , Daejeon 34141, Korea.
authors.forename: Ji Yu
authors.initials: JY
authors.lastname: Choi
authors.affiliation: Department of Chemistry, Sookmyung Women's University , Seoul 04310, Korea.
authors.forename: Da Hee
authors.initials: DH
authors.lastname: Jung
authors.affiliation: Department of Chemistry, National University of Singapore , Singapore 117543, Singapore.
authors.forename: Young-Tae
authors.initials: YT
authors.lastname: Chang
authors.affiliation: School of Materials Science and Engineering, Gwangju Institute of Science and Technology , Gwangju 61005, Korea.
authors.forename: Myung-Han
authors.initials: MH
authors.lastname: Yoon
authors.affiliation: Department of Chemistry, Sookmyung Women's University , Seoul 04310, Korea.
authors.forename: Jin Seok
authors.initials: JS
authors.lastname: Lee
authors.affiliation: Department of Bio and Brain Engineering, KAIST , Daejeon 34141, Korea.
authors.forename: Yoonkey
authors.initials: Y
authors.lastname: Nam
authors.affiliation: Center for Cell-Encapsulation Research and Molecular-Level Interface Research Center, Department of Chemistry, KAIST , Daejeon 34141, Korea.|Department of Bio and Brain Engineering, KAIST , Daejeon 34141, Korea.
authors.forename: Insung S
authors.initials: IS
authors.lastname: Choi
mesh_terms: D000199:Actins / Q000737:chemistry; D000818:Animals; D001369:Axons / Q000737:chemistry / Q000502:physiology; D018929:Cell Culture Techniques; D058948:Cell Tracking / Q000379:methods; D006624:Hippocampus / Q000737:chemistry* / Q000166:cytology; D053770:Nanowires / Q000737:chemistry*; D016501:Neurites / Q000737:chemistry*; D055495:Neurogenesis; D051381:Rats; D012825:Silicon / Q000737:chemistry
chemical_list: D000199:Actins; D012825:Silicon
keywords: F-actin dynamics; Silicon nanowires; chemical vapor deposition; live-cell imaging; neurite development; primary hippocampal neuron