title: Annular dark field transmission electron microscopy for protein structure determination.
abstract: Recently annular dark field (ADF) transmission electron microscopy (TEM) has been advocated as a means of recording images of biological specimens with better signal to noise ratio (SNR) than regular bright field images. I investigate whether and how such images could be used to determine the three-dimensional structure of proteins given that an ADF aperture with a suitable pass-band can be manufactured and used in practice. I develop an approximate theory of ADF-TEM image formation for weak amplitude and phase objects and test this theory using computer simulations. I also test whether these simulated images can be used to calculate a three-dimensional model of the protein using standard software and discuss problems and possible ways to overcome these.
authors.affiliation: Royal Institute of Technology, School of Technology and Health and Karolinska Institutet, Department of Biosciences and Nutrition, Novum, 14183 Huddinge, Sweden. Electronic address: Philip.Koeck@ki.se.
authors.forename: Philip J B
authors.initials: PJB
authors.lastname: Koeck
mesh_terms: D000465:Algorithms; D003198:Computer Simulation; D007091:Image Processing, Computer-Assisted; D021621:Imaging, Three-Dimensional; D046529:Microscopy, Electron, Transmission / Q000379:methods* / Q000706:statistics & numerical data; D008958:Models, Molecular; D011506:Proteins / Q000737:chemistry* / Q000648:ultrastructure*; D059629:Signal-To-Noise Ratio; D012984:Software
chemical_list: D011506:Proteins
keywords: Annular dark field aperture; Linear imaging; Phase contrast transfer function; Projection approximation; Resolution; Transmission electron microscopy; Weak phase and amplitude object approximation