title: A 2-Hydroxypyridine Catabolism Pathway in Rhodococcus rhodochrous Strain PY11.
abstract: Rhodococcus rhodochrous PY11 (DSM 101666) is able to use 2-hydroxypyridine as a sole source of carbon and energy. By investigating a gene cluster (hpo) from this bacterium, we were able to reconstruct the catabolic pathway of 2-hydroxypyridine degradation. Here, we report that in Rhodococcus rhodochrous PY11, the initial hydroxylation of 2-hydroxypyridine is catalyzed by a four-component dioxygenase (HpoBCDF). A product of the dioxygenase reaction (3,6-dihydroxy-1,2,3,6-tetrahydropyridin-2-one) is further oxidized by HpoE to 2,3,6-trihydroxypyridine, which spontaneously forms a blue pigment. In addition, we show that the subsequent 2,3,6-trihydroxypyridine ring opening is catalyzed by the hypothetical cyclase HpoH. The final products of 2-hydroxypyridine degradation in Rhodococcus rhodochrous PY11 are ammonium ion and α-ketoglutarate.
authors.affiliation: Department of Molecular Microbiology and Biotechnology, Institute of Biochemistry, Vilnius University, Vilnius, Lithuania justas.vaitekunas@bchi.vu.lt.
authors.forename: Justas
authors.initials: J
authors.lastname: Vaitekūnas
authors.affiliation: Department of Molecular Microbiology and Biotechnology, Institute of Biochemistry, Vilnius University, Vilnius, Lithuania.
authors.forename: Renata
authors.initials: R
authors.lastname: Gasparavičiūtė
authors.affiliation: Department of Molecular Microbiology and Biotechnology, Institute of Biochemistry, Vilnius University, Vilnius, Lithuania.
authors.forename: Rasa
authors.initials: R
authors.lastname: Rutkienė
authors.affiliation: Department of Molecular Microbiology and Biotechnology, Institute of Biochemistry, Vilnius University, Vilnius, Lithuania.
authors.forename: Daiva
authors.initials: D
authors.lastname: Tauraitė
authors.affiliation: Department of Molecular Microbiology and Biotechnology, Institute of Biochemistry, Vilnius University, Vilnius, Lithuania.
authors.forename: Rolandas
authors.initials: R
authors.lastname: Meškys
mesh_terms: D001711:Biotransformation; D002244:Carbon / Q000378:metabolism; D053858:Metabolic Networks and Pathways / Q000235:genetics*; D005810:Multigene Family; D011728:Pyridones / Q000378:metabolism*; D012240:Rhodococcus / Q000254:growth & development / Q000378:metabolism*
chemical_list: D011728:Pyridones; C017679:2-hydroxypyridine; D002244:Carbon