title: Allogeneic adipose-derived stem cells promote survival of fat grafts in immunocompetent diabetic rats.
abstract: Autologous adipose-derived stem cells (ADSCs) can protect fat grafts in cell-assisted lipotransfer (CAL). However, diabetes alters the intrinsic properties of ADSCs and impairs their function so that they lack these protective effects. We investigate whether allogeneic ADSCs from healthy donors could protect fat grafts in immunocompetent diabetic rats. Syngeniec adipose tissues and ADSCs were derived from diabetic Lewis (LEW) rats, whereas allogeneic ADSCs were from healthy brown-Norway rats. A grafted mixture containing 0.7 ml granule fat and 0.3 ml 6 × 10(6) allogeneic/syngeneic ADSCs was injected subcutaneously on the skulls of diabetic LEW rats. Fat samples were harvested to evaluate the levels of injury and vascularization as shown by perilipin A, CD34 and VEGF at 14 days. The immune response was evaluated with a lymphocytotoxicity test and the CD4/CD8 ratio in peripheral blood at 14 days. The volume retention of fat grafts was measured at 3 months. Healthy allogeneic ADSCs increased the expression levels of perilipin A, CD34 and VEGF at 14 days. The volume retention of fat grafts was improved by allogeneic ADSCs at 3 months. ADSCs were demonstrated to have low immunogenicity by the lymphocyte proliferation test and immunophenotype including MHC and co-stimulatory markers. The lymphocytotoxicity test and CD4/CD8 ratio indicated no obvious immune response elicited by allogeneic ADSCs. Thus, healthy allogeneic ADSCs can promote the survival of fat grafts in this immunocompetent diabetic rat model, with little or no obvious immune rejection.
authors.affiliation: Graduate School, Second Military Medical University, Shanghai, 200433, People's Republic of China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Burns and Cutaneous Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, 710032, People's Republic of China.
authors.forename: Xiaozhi
authors.initials: X
authors.lastname: Bai
authors.affiliation: Department of Burns and Cutaneous Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, 710032, People's Republic of China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhao
authors.affiliation: Department of Burns and Cutaneous Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, 710032, People's Republic of China.
authors.forename: Yunchuan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Burns and Cutaneous Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, 710032, People's Republic of China.
authors.forename: Linlin
authors.initials: L
authors.lastname: Su
authors.affiliation: Department of Plastic Surgery, General Hospital of Shenyang Military Area Command of PLA, 83 Wenhua Road, Shenyang, Liaoning, 110016, People's Republic of China.
authors.forename: Peng
authors.initials: P
authors.lastname: Chang
authors.affiliation: Department of Burns and Cutaneous Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, 710032, People's Republic of China.
authors.forename: Xujie
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Burns and Cutaneous Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, 710032, People's Republic of China.
authors.forename: Shichao
authors.initials: S
authors.lastname: Han
authors.affiliation: Department of Burns and Cutaneous Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, 710032, People's Republic of China.
authors.forename: Jianxin
authors.initials: J
authors.lastname: Gao
authors.affiliation: Department of Burns and Cutaneous Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, 710032, People's Republic of China.
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Hu
authors.affiliation: Department of Burns and Cutaneous Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, 710032, People's Republic of China.
authors.forename: Dahai
authors.initials: D
authors.lastname: Hu
authors.affiliation: Graduate School, Second Military Medical University, Shanghai, 200433, People's Republic of China. liuxyan2013@sohu.com.|Department of Plastic Surgery, General Hospital of Shenyang Military Area Command of PLA, 83 Wenhua Road, Shenyang, Liaoning, 110016, People's Republic of China. liuxyan2013@sohu.com.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Liu
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000637:transplantation*; D000273:Adipose Tissue / Q000166:cytology / Q000637:transplantation*; D000818:Animals; D016516:CD4-CD8 Ratio; D049109:Cell Proliferation; D002478:Cells, Cultured; D003920:Diabetes Mellitus / Q000473:pathology; D003921:Diabetes Mellitus, Experimental / Q000473:pathology*; D006085:Graft Survival / Q000502:physiology*; D008297:Male; D000070778:Perilipin-1 / Q000378:metabolism; D051381:Rats; D011914:Rats, Inbred BN; D011917:Rats, Inbred Lew; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology; D014184:Transplantation, Homologous
chemical_list: D000070778:Perilipin-1
keywords: Allogeneic stem cell; Autologous adipose-derived stem cells; Cell transplantation; Fat graft; Immune response