title: Functional characterization of Mammary Gland Protein-40, a chitinase-like glycoprotein expressed during mammary gland apoptosis.
abstract: MGP-40 is a chitinase-like protein which is over expressed during mammary gland involution. However, its physiological function in the mammary gland is poorly understood. In the present investigation, we have reported the functional significance of buffalo specific MGP-40 in the mammary gland by using an in vitro model of the buffalo mammary epithelial cell (BuMEC) line. MGP-40 was highly up regulated in BuMECs in serum starved condition as well as after treatment with prolactin suggesting its role in the stress response. Subsequently, to study the effect of MGP-40 on BuMECs, the cells were transfected with a mammalian expression construct of pCI neo harboring MGP-40 gene. It was observed that over expression of MGP-40 enhanced proliferation of BuMECs and protected the cells from apoptosis under serum free condition. In contrast, MGP-40 attenuated the mitogenic effect of insulin in BuMECs. Besides, over expression of the MGP-40 reduced dome formation, acinar polarization and casein synthesis in BuMECs in the presence of lactogenic hormones, it also induced Stat3 phosphorylation and epithelial to mesenchymal transition (EMT) -like features. Together, our data suggest that MGP-40 is involved in protection of BuMECs under stress conditions, inhibits cellular differentiation and induces EMT-like features. A schematic diagram depicting possible association of MGP-40 in various molecular pathways has been presented.
authors.affiliation: Animal Biotechnology Centre, National Dairy Research Institute (NDRI), Karnal, 132001, India.
authors.forename: Vijay
authors.initials: V
authors.lastname: Anand
authors.affiliation: Animal Biotechnology Centre, National Dairy Research Institute (NDRI), Karnal, 132001, India.
authors.forename: Shalini
authors.initials: S
authors.lastname: Jaswal
authors.affiliation: Animal Biotechnology Centre, National Dairy Research Institute (NDRI), Karnal, 132001, India.
authors.forename: Surender
authors.initials: S
authors.lastname: Singh
authors.affiliation: Animal Biotechnology Centre, National Dairy Research Institute (NDRI), Karnal, 132001, India.
authors.forename: Sudarshan
authors.initials: S
authors.lastname: Kumar
authors.affiliation: Animal Biotechnology Centre, National Dairy Research Institute (NDRI), Karnal, 132001, India.
authors.forename: Manoj Kumar
authors.initials: MK
authors.lastname: Jena
authors.affiliation: Animal Biotechnology Centre, National Dairy Research Institute (NDRI), Karnal, 132001, India.
authors.forename: Arvind Kumar
authors.initials: AK
authors.lastname: Verma
authors.affiliation: Animal Biotechnology Centre, National Dairy Research Institute (NDRI), Karnal, 132001, India.
authors.forename: Munna Lal
authors.initials: ML
authors.lastname: Yadav
authors.affiliation: Department of Physiology, University of Tennessee Health Science Center, Memphis, TN, 38163, USA.
authors.forename: Jagadeesh
authors.initials: J
authors.lastname: Janjanam
authors.affiliation: Animal Biotechnology Centre, National Dairy Research Institute (NDRI), Karnal, 132001, India.
authors.forename: Masoud
authors.initials: M
authors.lastname: Lotfan
authors.affiliation: Animal Biotechnology Centre, National Dairy Research Institute (NDRI), Karnal, 132001, India.
authors.forename: Dhruba
authors.initials: D
authors.lastname: Malakar
authors.affiliation: Dairy Cattle Physiology Division, National Dairy Research Institute (NDRI), Karnal, 132001, India.
authors.forename: Ajay Kumar
authors.initials: AK
authors.lastname: Dang
authors.affiliation: Livestock Production and Management Division, National Dairy Research Institute (NDRI), Karnal, 132001, India.
authors.forename: Tushar Kumar
authors.initials: TK
authors.lastname: Mohanty
authors.affiliation: Animal Biotechnology Centre, National Dairy Research Institute (NDRI), Karnal, 132001, India.
authors.forename: Jai Kumar
authors.initials: JK
authors.lastname: Kaushik
authors.affiliation: Animal Biotechnology Centre, National Dairy Research Institute (NDRI), Karnal, 132001, India. akm@ndri.res.in.
authors.forename: Ashok Kumar
authors.initials: AK
authors.lastname: Mohanty
mesh_terms: D000818:Animals; D017209:Apoptosis; D002020:Buffaloes; D002364:Caseins / Q000235:genetics / Q000378:metabolism; D016764:Cell Polarity; D049109:Cell Proliferation; D048430:Cell Shape; D002478:Cells, Cultured; D002688:Chitinases / Q000235:genetics / Q000378:metabolism; D004847:Epithelial Cells / Q000502:physiology*; D005260:Female; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism*; D008321:Mammary Glands, Animal / Q000166:cytology; D011388:Prolactin / Q000502:physiology; D015533:Transcriptional Activation
chemical_list: D002364:Caseins; D006023:Glycoproteins; D011388:Prolactin; D002688:Chitinases
keywords: Buffalo Mammary Epithelial cells; Cytoprotection; Epithelial Mesenchymal Transition; Prolactin; Stat3