title: Efficient purification of lysozyme from egg white by 2-mercapto-5-benzimidazolesulfonic acid modified Fe3O4/Au nanoparticles.
abstract: 2-Mercapto-5-benzimidazolesulfonic acid (MBISA) modified Fe3O4/Au nanoparticles were synthesized in aqueous solution and characterized by photo correlation spectroscopy (PCS) and vibrating sample magnetometer (VSM). The so-obtained Fe3O4/Au-MBISA nanoparticles were capable of specific adsorbing lysozyme. The maximum amount of lysozyme adsorbed on 1.0mg Fe3O4/Au-MBISA nanoparticles was 346μg. The lysozyme desorption behavior was studied and the lysozyme recovery from Fe3O4/Au-MBISA nanoparticles approached 100% under optimal conditions, and the reusability studies showed that the nanoparticles could maintain about 91% of the initial lysozyme adsorption capacity after 7 repeated adsorption-elution cycles. The Fe3O4/Au-MBISA nanoparticles were used in the purification of lysozyme from chicken egg white, which was verified by a single SDS-PAGE band. Therefore, the obtained Fe3O4/Au-MBISA nanoparticles exhibited excellent performance in the direct purification of lysozyme from egg white.
authors.affiliation: Shandong Province Collegial Key Laboratory of Biotechnology and Biological Resources Utilization, College of Life Science, Dezhou University, Dezhou 253023, PR China.
authors.forename: Xinjun
authors.initials: X
authors.lastname: Zhu
authors.affiliation: Shandong Province Collegial Key Laboratory of Biotechnology and Biological Resources Utilization, College of Life Science, Dezhou University, Dezhou 253023, PR China. Electronic address: lianyingzh@yahoo.com.
authors.forename: Lianying
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Shandong Province Collegial Key Laboratory of Biotechnology and Biological Resources Utilization, College of Life Science, Dezhou University, Dezhou 253023, PR China.
authors.forename: Aiyun
authors.initials: A
authors.lastname: Fu
authors.affiliation: Shandong Province Collegial Key Laboratory of Biotechnology and Biological Resources Utilization, College of Life Science, Dezhou University, Dezhou 253023, PR China.
authors.forename: Hao
authors.initials: H
authors.lastname: Yuan
mesh_terms: D000818:Animals; D030161:Avian Proteins / Q000737:chemistry / Q000302:isolation & purification; D001562:Benzimidazoles / Q000737:chemistry*; D002645:Chickens; D004527:Egg Proteins / Q000737:chemistry*; D052203:Ferrosoferric Oxide / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D009113:Muramidase / Q000737:chemistry / Q000302:isolation & purification; D053758:Nanoparticles / Q000737:chemistry*; D013451:Sulfonic Acids / Q000737:chemistry*
chemical_list: C497089:2-mercapto-5-benzimidazolesulfonic acid; D030161:Avian Proteins; D001562:Benzimidazoles; D004527:Egg Proteins; D013451:Sulfonic Acids; D006046:Gold; D009113:Muramidase; D052203:Ferrosoferric Oxide
keywords: 2-Mercapto-5-benzimidazolesulfonic acid; Fe(3)O(4)/Au nanoparticles; Lysozyme; Purification