title: Efficient development of polymorphic microsatellite loci for Pteroceltis tatarinowii (Ulmaceae).
abstract: Pteroceltis tatarinowii (Ulmaceae) is a scientifically and economically important temperate deciduous tree that is endemic to China. In the present study, 12 P. tatarinowii polymorphic microsatellite loci were developed using the tailed primer-M13-simple sequence repeats (TP-M13-SSR) biotin-capture method. The number of alleles per locus ranged from 2 to 10, with an average of 6.58. The observed and expected heterozygosity ranged from 0.208 to 0.958 and from 0.198 to 0.858, with average values of 0.703 and 0.710, respectively. The markers isolated in this study represent a favorable tool for further analyses of the population genetic structure and evolutionary history of this relic tree.
authors.affiliation: College of Life Science, Anhui Normal University, Wuhu, China.
authors.forename: X H
authors.initials: XH
authors.lastname: Li
authors.affiliation: College of Life Science, Anhui Normal University, Wuhu, China pinghengxu@sina.com.cn.
authors.forename: X P
authors.initials: XP
authors.lastname: Zhang
authors.affiliation: College of Life Science, Anhui Normal University, Wuhu, China.
authors.forename: K
authors.initials: K
authors.lastname: Liu
authors.affiliation: College of Life Science, Anhui Normal University, Wuhu, China.
authors.forename: H J
authors.initials: HJ
authors.lastname: Liu
authors.affiliation: College of Life Science, Anhui Normal University, Wuhu, China.
authors.forename: J W
authors.initials: JW
authors.lastname: Shao
mesh_terms: D056426:Genetic Loci; D005828:Genetics, Population; D018895:Microsatellite Repeats; D059372:Nucleotide Motifs; D011110:Polymorphism, Genetic; D034401:Ulmaceae / Q000235:genetics*