title: Serological Responses to a Norovirus Nonstructural Fusion Protein after Vaccination and Infection.
abstract: The performance of an assay to detect antibodies to a norovirus nonstructural fusion protein, designated VPR and consisting of three proteins (GI.1 virus protein genome-linked [VPg], a virus protease, and an RNA-dependent RNA polymerase), was evaluated. The assay sensitivity and specificity were 74.5% and >95%, respectively, for identifying GI.1 norovirus infection among persons who received either a monovalent GI.1 norovirus virus-like particle (VLP) vaccine or placebo by the intranasal route followed by an oral live GI.1 norovirus challenge.
authors.affiliation: Department of Medicine, Baylor College of Medicine, Houston, Texas, USA.
authors.forename: Shayla
authors.initials: S
authors.lastname: Hesse
authors.affiliation: Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas, USA.
authors.forename: Frederick H
authors.initials: FH
authors.lastname: Neill
authors.affiliation: Department of Medicine, Baylor College of Medicine, Houston, Texas, USA Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas, USA.
authors.forename: Mary K
authors.initials: MK
authors.lastname: Estes
authors.affiliation: Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas, USA.
authors.forename: Sreejesh
authors.initials: S
authors.lastname: Shanker
authors.affiliation: Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas, USA Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas, USA.
authors.forename: B V Venkataram
authors.initials: BV
authors.lastname: Prasad
authors.affiliation: EMMES Corporation, Rockville, Maryland, USA.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Ferreira
authors.affiliation: Department of Medicine, Baylor College of Medicine, Houston, Texas, USA Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas, USA ratmar@bcm.edu.
authors.forename: Robert L
authors.initials: RL
authors.lastname: Atmar
mesh_terms: D000281:Administration, Intranasal; D000914:Antibodies, Viral / Q000097:blood* / Q000276:immunology; D017250:Caliciviridae Infections / Q000175:diagnosis / Q000276:immunology* / Q000821:virology; D005243:Feces / Q000821:virology; D006801:Humans; D007118:Immunoassay; D029322:Norovirus / Q000737:chemistry / Q000276:immunology*; D012680:Sensitivity and Specificity; D058425:Vaccines, Virus-Like Particle / Q000008:administration & dosage / Q000276:immunology; D014760:Viral Fusion Proteins / Q000276:immunology; D017361:Viral Nonstructural Proteins / Q000276:immunology*; D014765:Viral Vaccines / Q000008:administration & dosage* / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D058425:Vaccines, Virus-Like Particle; D014760:Viral Fusion Proteins; D017361:Viral Nonstructural Proteins; D014765:Viral Vaccines