title: Mesenchymal stem cell-laden anti-inflammatory hydrogel enhances diabetic wound healing.
abstract: The purpose of this study was to permit bone marrow mesenchymal stem cells (BMSCs) to reach their full potential in the treatment of chronic wounds. A biocompatible multifunctional crosslinker based temperature sensitive hydrogel was developed to deliver BMSCs, which improve the chronic inflammation microenvironments of wounds. A detailed in vitro investigation found that the hydrogel is suitable for BMSC encapsulation and can promote BMSC secretion of TGF-β1 and bFGF. In vivo, full-thickness skin defects were made on the backs of db/db mice to mimic diabetic ulcers. It was revealed that the hydrogel can inhibit pro-inflammatory M1 macrophage expression. After hydrogel association with BMSCs treated the wound, significantly greater wound contraction was observed in the hydrogel + BMSCs group. Histology and immunohistochemistry results confirmed that this treatment contributed to the rapid healing of diabetic skin wounds by promoting granulation tissue formation, angiogenesis, extracellular matrix secretion, wound contraction, and re-epithelialization. These results show that a hydrogel laden with BMSCs may be a promising therapeutic strategy for the management of diabetic ulcers.
authors.affiliation: Department of Histology and Embryology, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, China.
authors.forename: Shixuan
authors.initials: S
authors.lastname: Chen
authors.affiliation: Departments of Mechanical Engineering, Biochemistry and Medical Genetics, University of Manitoba, and Manitoba Institute of Child Health, Winnipeg, MB R3T 2N2, Canada.
authors.forename: Junbin
authors.initials: J
authors.lastname: Shi
authors.affiliation: Department of Histology and Embryology, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Department of Histology and Embryology, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, China.
authors.forename: Yinghua
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Histology and Embryology, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, China.
authors.forename: Xueer
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Histology and Embryology, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Histology and Embryology, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, China.
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Tian
authors.affiliation: Department of Histology and Embryology, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yan
authors.affiliation: Department of Histology and Embryology, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, China.
authors.forename: Qinglin
authors.initials: Q
authors.lastname: Li
authors.affiliation: Department of Textile Sciences, Department of Medical Biology, University of Manitoba, Winnipeg, MB R3T 2N2, Canada.
authors.forename: Wen
authors.initials: W
authors.lastname: Zhong
authors.affiliation: Departments of Mechanical Engineering, Biochemistry and Medical Genetics, University of Manitoba, and Manitoba Institute of Child Health, Winnipeg, MB R3T 2N2, Canada.
authors.forename: Malcolm
authors.initials: M
authors.lastname: Xing
authors.affiliation: Key Laboratory of Functional Proteomics of Guangdong Province, Department of Pathophysiology, Southern Medical University, Guangzhou 510515, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Histology and Embryology, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000627:therapeutic use; D048909:Diabetes Complications / Q000473:pathology* / Q000628:therapy; D004195:Disease Models, Animal; D006097:Granulation Tissue / Q000378:metabolism / Q000473:pathology; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000138:chemical synthesis / Q000737:chemistry; D008297:Male; D008422:Materials Testing; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D051379:Mice; D012883:Skin Ulcer / Q000209:etiology / Q000473:pathology* / Q000628:therapy; D014945:Wound Healing
chemical_list: D001672:Biocompatible Materials; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate