title: Testing the variability of PSA expression by different human prostate cancer cell lines by means of a new potentiometric device employing molecularly antibody assembled on graphene surface.
abstract: Prostate Specific Antigen (PSA) is widely used as a biomarker for prostate cancer. Recently, an electrochemical biosensor for PSA detection by means of molecularly imprinted polymers (MIPs) was developed. This work evaluated the performance and the effectiveness of that PSA biosensor in screening the biomarker PSA in biological media with complex composition, collected from different human prostate cell line cultures. For that, the prostate cancer LNCaP and PC3 cells, and the non-cancerous prostate cell line PNT2 were cultured for 2, 7 and 14days in either α-MEM or RPMI in the presence of 10% or 30% fetal bovine serum. Human gingival fibroblasts were used as a non-cancerous non-prostatic control. The different culture conditions modulated cellular proliferation and the expression of several prostate markers, including PSA. The electrochemical biosensor was able to specifically detect PSA in the culture media and values obtained were similar to those achieved by a commercial Enzyme-Linked Immunosorbent Assay (ELISA) kit, the most commonly used method for PSA quantification in prostate cancer diagnosis. Thus, the tested biosensor may represent a useful alternative as a diagnostic tool for PSA determination in biological samples.
authors.affiliation: BioMark-CINTESIS/ISEP, Instituto Superior de Engenharia do Instituto Politécnico do Porto, Portugal; LAQV, REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Caparica, Portugal; Laboratory for Bone Metabolism and Regeneration, Faculdade de Medicina Dentária, Universidade do Porto, Porto, Portugal.
authors.forename: Tânia S C R
authors.initials: TSCR
authors.lastname: Rebelo
authors.affiliation: LAQV, REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Caparica, Portugal.
authors.forename: João P
authors.initials: JP
authors.lastname: Noronha
authors.affiliation: LAQV, REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Caparica, Portugal.
authors.forename: Marco
authors.initials: M
authors.lastname: Galésio
authors.affiliation: LAQV, REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Caparica, Portugal.
authors.forename: Hugo
authors.initials: H
authors.lastname: Santos
authors.affiliation: LAQV, REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Caparica, Portugal.
authors.forename: Mário
authors.initials: M
authors.lastname: Diniz
authors.affiliation: BioMark-CINTESIS/ISEP, Instituto Superior de Engenharia do Instituto Politécnico do Porto, Portugal.
authors.forename: M Goreti F
authors.initials: MGF
authors.lastname: Sales
authors.affiliation: Laboratory for Bone Metabolism and Regeneration, Faculdade de Medicina Dentária, Universidade do Porto, Porto, Portugal.
authors.forename: Maria H
authors.initials: MH
authors.lastname: Fernandes
authors.affiliation: Laboratory for Bone Metabolism and Regeneration, Faculdade de Medicina Dentária, Universidade do Porto, Porto, Portugal; ESTSP - Escola Superior de Tecnologia da Saúde do Porto, Instituto Politécnico do Porto, Portugal. Electronic address: jrodrigues@fmd.up.pt.
authors.forename: João
authors.initials: J
authors.lastname: Costa-Rodrigues
mesh_terms: D000328:Adult; D015374:Biosensing Techniques / Q000295:instrumentation*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D004867:Equipment Design; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D008297:Male; D011199:Potentiometry / Q000295:instrumentation*; D017430:Prostate-Specific Antigen / Q000378:metabolism*; D011471:Prostatic Neoplasms / Q000378:metabolism*; D013499:Surface Properties
chemical_list: D006108:Graphite; D017430:Prostate-Specific Antigen
keywords: Electrochemical biosensor; Prostate Specific Antigen; Prostate cell lines