title: Fluorescence Probing Live Single-cell Mass Spectrometry for Direct Analysis of Organelle Metabolism.
abstract: Mitochondria in a live HepG2 cell were visualized with a fluorescent probe to specify their location and state in a living cell. Then, mitochondria were selectively captured with a nanospray tip under fluorescence microscope, and thousands of small molecular peaks were revealed and unique steroids specific to mitochondria were also found. This fluorescence imaging combined with live single-cell mass spectrometry opens the door to the analysis of site- and state-specific molecular detection to elucidate precise molecular mechanisms at the single-cell and organelle level.
authors.affiliation: Quantitative Biology Center (QBiC), RIKEN.
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Esaki
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Masujima
mesh_terms: D005456:Fluorescent Dyes / Q000737:chemistry*; D056945:Hep G2 Cells; D006801:Humans; D018613:Microscopy, Confocal / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D008928:Mitochondria / Q000378:metabolism*; D025341:Principal Component Analysis; D059010:Single-Cell Analysis / Q000295:instrumentation / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes