title: Cutting Edge: Role of NK Cells and Surfactant Protein D in Dendritic Cell Lymph Node Homing: Effects of Ozone Exposure.
abstract: The roles of NK cells, surfactant protein D (SP-D), and IFN-γ, as well as the effect of ozone (O3) inhalation, were studied on recirculation of pulmonary dendritic cells (DC) to the mediastinal lymph nodes. O3 exposure and lack of SP-D reduced NK cell IFN-γ and lung tissue CCL21 mRNA expression and impaired DC homing to the mediastinal lymph nodes. Notably, addition of recombinant SP-D to naive mononuclear cells stimulated IFN-γ release in vitro. Because NKp46, a glycosylated membrane receptor, was necessary for dose-dependent SP-D binding to NK cells in vitro and DC migration in vivo, we speculate that SP-D may constitutively stimulate IFN-γ production by NK cells, possibly via NKp46. This mechanism could then initiate the IFN-γ/IL-12 feedback circuit, a key amplifier of DC lymph node homing. Inhibition of this process during an acute inflammatory response causes DC retention in the peripheral lung tissue and contributes to injury.
authors.affiliation: University of California, Davis, Davis, CA 95616; University of Pennsylvania, Philadelphia, PA 19104; National University of Singapore, Singapore 117456; and.
authors.forename: Moyar Qing
authors.initials: MQ
authors.lastname: Ge
authors.affiliation: University of Pennsylvania, Philadelphia, PA 19104;
authors.forename: Blerina
authors.initials: B
authors.lastname: Kokalari
authors.affiliation: University of California, Davis, Davis, CA 95616;
authors.forename: Cameron H
authors.identifier: http://orcid.org/0000-0002-3001-9058
authors.initials: CH
authors.lastname: Flayer
authors.affiliation: University of California, Davis, Davis, CA 95616;
authors.forename: Sarah S
authors.identifier: http://orcid.org/0000-0002-8643-0778
authors.initials: SS
authors.lastname: Killingbeck
authors.affiliation: University of Pennsylvania, Philadelphia, PA 19104;
authors.forename: Imre G
authors.initials: IG
authors.lastname: Redai
authors.affiliation: Fox Chase Cancer Center, Philadelphia, PA 19111.
authors.forename: Alexander W
authors.initials: AW
authors.lastname: MacFarlane
authors.affiliation: University of Pennsylvania, Philadelphia, PA 19104;
authors.forename: Jin W
authors.identifier: http://orcid.org/0000-0002-4635-7342
authors.initials: JW
authors.lastname: Hwang
authors.affiliation: University of Pennsylvania, Philadelphia, PA 19104;
authors.forename: Anisha
authors.initials: A
authors.lastname: Kolupoti
authors.affiliation: National University of Singapore, Singapore 117456; and.
authors.forename: Michael D
authors.identifier: http://orcid.org/0000-0001-7964-6151
authors.initials: MD
authors.lastname: Kemeny
authors.affiliation: Fox Chase Cancer Center, Philadelphia, PA 19111.
authors.forename: Kerry S
authors.initials: KS
authors.lastname: Campbell
authors.affiliation: University of California, Davis, Davis, CA 95616; University of Pennsylvania, Philadelphia, PA 19104; haczku@ucdavis.edu.
authors.forename: Angela
authors.identifier: http://orcid.org/0000-0002-1301-4941
authors.initials: A
authors.lastname: Haczku
mesh_terms: D000818:Animals; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D003713:Dendritic Cells / Q000276:immunology*; D005434:Flow Cytometry; D007371:Interferon-gamma; D007694:Killer Cells, Natural / Q000276:immunology*; D008168:Lung / Q000276:immunology; D008198:Lymph Nodes / Q000276:immunology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010126:Ozone / Q000633:toxicity*; D011014:Pneumonia / Q000276:immunology; D037663:Pulmonary Surfactant-Associated Protein D / Q000276:immunology*; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D037663:Pulmonary Surfactant-Associated Protein D; D010126:Ozone; D007371:Interferon-gamma