title: Transient receptor potential vanilloid 1 expression and function in splenic dendritic cells: a potential role in immune homeostasis.
abstract: Neuro-immune interactions, particularly those driven by neuropeptides, are increasingly implicated in immune responses. For instance, triggering calcium-channel transient receptor potential vanilloid 1 (TRPV1) on sensory nerves induces the release of calcitonin-gene-related peptide (CGRP), a neuropeptide known to moderate dendritic cell activation and T helper cell type 1 polarization. Despite observations that CGRP is not confined to the nervous system, few studies have addressed the possibility that immune cells can respond to well-documented 'neural' ligands independently of peripheral nerves. Here we have identified functionally relevant TRPV1 on primary antigen-presenting cells of the spleen and have demonstrated both calcium influx and CGRP release in three separate strains of mice using natural agonists. Furthermore, we have shown down-regulation of activation markers CD80/86 on dendritic cells, and up-regulation of interleukin-6 and interleukin-10 in response to CGRP treatment. We suggest that dendritic cell responses to neural ligands can amplify neuropeptide release, but more importantly that variability in CGRP release across individuals may have important implications for immune cell homeostasis.
authors.affiliation: Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, Saudi Arabia.|Visiting Scientist, Faculty of Medicine and Human Sciences, University of Manchester, Manchester, UK.
authors.forename: Mushref Bakri
authors.initials: MB
authors.lastname: Assas
authors.affiliation: Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, Saudi Arabia.
authors.forename: Majed H
authors.initials: MH
authors.lastname: Wakid
authors.affiliation: Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, Saudi Arabia.
authors.forename: Haytham A
authors.initials: HA
authors.lastname: Zakai
authors.affiliation: Faculty of Life Sciences, University of Manchester, Manchester, UK.
authors.forename: Jaleel A
authors.initials: JA
authors.lastname: Miyan
authors.affiliation: Institute of Inflammation & Repair, Faculty of Medicine and Human Sciences, University of Manchester, Manchester, UK.
authors.forename: Joanne L
authors.initials: JL
authors.lastname: Pennock
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018920:Coculture Techniques; D003713:Dendritic Cells / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D006706:Homeostasis / Q000276:immunology*; D008213:Lymphocyte Activation / Q000276:immunology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D015213:Neuroimmunomodulation / Q000276:immunology*; D013154:Spleen / Q000166:cytology / Q000276:immunology; D050916:TRPV Cation Channels / Q000276:immunology*
chemical_list: D050916:TRPV Cation Channels; C493921:TRPV1 protein, mouse
keywords: calcitonin-gene-related peptide; dendritic cell; lymph node; transient receptor potential vanilloid 1