title: A Simple Liquid Chromatography Tandem Mass Spectrometry Method for Quantitation of Plasma Busulfan.
abstract: Busulfan is an alkylating agent widely used in the ablation of bone marrow cells before hematopoietic stem cell transplant. Due to large intraindividual and interindividual variations, and narrow therapeutic window, therapeutic drug monitoring of busulfan is warranted. A quick and reliable HPLC-MS/MS method was developed for the assay of plasma busulfan. HPLC involved C18 column, and MS/MS was used in electrospray ionization (ESI) positive mode. Quantitation and identification of busulfan was made using various multiple reactions monitoring (MRMs). Isotopic labeled busulfan-d8 was used as the internal standard. The method is linear from 50 to 2500 ng/mL and has with-in run and between-run imprecision of <10 %.
authors.affiliation: Department of Pathology and Laboratory Medicine, Children's Mercy Hospitals and Clinics, 2401 Gillham Road, Kansas City, MO, USA.|Department of Pediatrics, Children's Mercy Hospitals and Clinics, Kansas City, MO, USA.
authors.forename: Shuang
authors.initials: S
authors.lastname: Deng
authors.affiliation: Department of Pathology and Laboratory Medicine, Children's Mercy Hospitals and Clinics, 2401 Gillham Road, Kansas City, MO, USA.
authors.forename: Michael
authors.initials: M
authors.lastname: Kiscoan
authors.affiliation: Department of Pathology and Laboratory Medicine, Children's Mercy Hospitals and Clinics, 2401 Gillham Road, Kansas City, MO, USA.
authors.forename: Clint
authors.initials: C
authors.lastname: Frazee
authors.affiliation: Department of Pediatrics, Children's Mercy Hospitals and Clinics, Kansas City, MO, USA.
authors.forename: Susan
authors.initials: S
authors.lastname: Abdel-Rahman
authors.affiliation: Department of Pediatrics, Children's Mercy Hospitals and Clinics, Kansas City, MO, USA.
authors.forename: Jignesh
authors.initials: J
authors.lastname: Dalal
authors.affiliation: Department of Pathology and Laboratory Medicine, Children's Mercy Hospitals and Clinics, 2401 Gillham Road, Kansas City, MO, USA. ugarg@cmh.edu.
authors.forename: Uttam
authors.initials: U
authors.lastname: Garg
mesh_terms: D002066:Busulfan / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000191:economics / Q000379:methods*; D016903:Drug Monitoring / Q000191:economics / Q000379:methods; D006801:Humans; D007166:Immunosuppressive Agents / Q000097:blood*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000191:economics / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000191:economics / Q000379:methods*
chemical_list: D007166:Immunosuppressive Agents; D002066:Busulfan
keywords: Bone marrow transplant; Busulfan; Leukemia; Liquid chromatography; Mass spectrometry