title: Comparison of structural changes in skin and amnion tissue grafts for transplantation induced by gamma and electron beam irradiation for sterilization.
abstract: Sterilization is an important step in the preparation of biological material for transplantation. The aim of the study is to compare morphological changes in three types of biological tissues induced by different doses of gamma and electron beam radiation. Frozen biological tissues (porcine skin xenografts, human skin allografts and human amnion) were irradiated with different doses of gamma rays (12.5, 25, 35, 50 kGy) and electron beam (15, 25, 50 kGy). Not irradiated specimens served as controls. The tissue samples were then thawn and fixed in 10 % formalin, processed by routine paraffin technique and stained with hematoxylin and eosin, alcian blue at pH 2.5, orcein, periodic acid Schiff reaction, phosphotungstic acid hematoxylin, Sirius red and silver impregnation. The staining with hematoxylin and eosin showed vacuolar cytoplasmic changes of epidermal cells mainly in the samples of xenografts irradiated by the lowest doses of gamma and electron beam radiation. The staining with orcein revealed damage of fine elastic fibers in the xenograft dermis at the dose of 25 kGy of both radiation types. Disintegration of epithelial basement membrane, especially in the xenografts, was induced by the dose of 15 kGy of electron beam radiation. The silver impregnation disclosed nuclear chromatin condensation mainly in human amnion at the lowest doses of both radiation types and disintegration of the fine collagen fibers in the papillary dermis induced by the lowest dose of electron beam and by the higher doses of gamma radiation. Irradiation by both, gamma rays and the electron beam, causes similar changes on cells and extracellular matrix, with significant damage of the basement membrane and of the fine and elastic and collagen fibers in the papillary dermis, the last caused already by low dose electron beam radiation.
authors.affiliation: Department of Pathology, Comenius University, Bratislava, Slovak Republic.
authors.forename: H
authors.initials: H
authors.lastname: Mrázová
authors.affiliation: Department of Burns and Reconstruction Surgery, Faculty of Medicine, Comenius University, Bratislava, Slovak Republic.
authors.forename: J
authors.initials: J
authors.lastname: Koller
authors.affiliation: Department of Pathology, Comenius University, Bratislava, Slovak Republic.
authors.forename: K
authors.initials: K
authors.lastname: Kubišová
authors.affiliation: Department of Pathology, Comenius University, Bratislava, Slovak Republic.
authors.forename: G
authors.initials: G
authors.lastname: Fujeríková
authors.affiliation: Department of Pathology, Comenius University, Bratislava, Slovak Republic.
authors.forename: E
authors.initials: E
authors.lastname: Klincová
authors.affiliation: Department of Pathology, Comenius University, Bratislava, Slovak Republic. pavel.babal@fmed.uniba.sk.
authors.forename: P
authors.initials: P
authors.lastname: Babál
mesh_terms: D000650:Amnion / Q000528:radiation effects* / Q000637:transplantation*; D000818:Animals; D004583:Electrons; D005720:Gamma Rays; D064593:Heterografts / Q000528:radiation effects; D006801:Humans; D012867:Skin / Q000033:anatomy & histology* / Q000528:radiation effects*; D016038:Skin Transplantation; D013242:Sterilization / Q000379:methods*; D034421:Sus scrofa
keywords: Allografts; Amnion; Electron beam; Gamma radiation; Skin; Sterilization; Xenografts