title: miR-22 inhibits the proliferation, motility, and invasion of human glioblastoma cells by directly targeting SIRT1.
abstract: Recently, microRNAs (miRNAs), a kind of small and non-coding RNA, can target the downstream molecules. Increasing evidence demonstrates that miRNAs meditate the onset and progression of a variety of tumors. In the present study, we carried out gene transfection, western blot, and reverse transcription PCR (RT-PCR) to explore the role of miR-22 in glioblastoma tissues and cell lines. Here, we verified that the expression of miR-22 was downregulated in glioblastoma tissues and cells rather than matched non-tumor tissues and normal human astrocyte (NHA) cells (p < 0.001). By contrast, SIRT1 messenger RNA (mRNA) and protein were upregulated in glioblastoma tissues and cells (p < 0.001). In vitro miR-22 mimics interfered with cell proliferation, migration, and invasion of U87 and U251 cells. Mechanically, the 3'-untranslated regions (3'-UTRs) of SIRT1 were a direct target of miR-22, leading to the decreased expression of SIRT1 protein in U87 and U251 cells. Meanwhile, miR-22 mimics also inhibited the expression of epidermal growth factor receptor (EGFR) and matrix metallopeptidase 9 (MMP9). In conclusion, miR-22 inhibited cell proliferation, migration, and invasion via targeting the 3'-UTR of SIRT1 in the progression of glioblastoma and miR-22-SIRT1 pathway can be recommended as a potential target for treatment of glioblastoma.
authors.affiliation: Department of Neurosurgery, Suzhou Kowloon Hospital, Shanghai Jiao Tong University School of Medicine, Suzhou, Jiangsu, 215021, People's Republic of China.
authors.forename: Hanchun
authors.initials: H
authors.lastname: Chen
authors.affiliation: Department of Laboratory Medicine, Changhai Hospital, Second Military Medical University, Shanghai, 200433, People's Republic of China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Lu
authors.affiliation: Department of Neurosurgery, Suzhou Kowloon Hospital, Shanghai Jiao Tong University School of Medicine, Suzhou, Jiangsu, 215021, People's Republic of China.
authors.forename: Xifeng
authors.initials: X
authors.lastname: Fei
authors.affiliation: Department of Neurosurgery, Suzhou Kowloon Hospital, Shanghai Jiao Tong University School of Medicine, Suzhou, Jiangsu, 215021, People's Republic of China.
authors.forename: Likui
authors.initials: L
authors.lastname: Shen
authors.affiliation: Department of Neurosurgery, Suzhou Kowloon Hospital, Shanghai Jiao Tong University School of Medicine, Suzhou, Jiangsu, 215021, People's Republic of China.
authors.forename: Dongyi
authors.initials: D
authors.lastname: Jiang
authors.affiliation: Department of Neurosurgery, Changhai Hospital, Second Military Medical University, Shanghai, 200433, People's Republic of China. daidwshanghai@163.com.
authors.forename: Dongwei
authors.initials: D
authors.lastname: Dai
mesh_terms: D020413:3' Untranslated Regions; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D005909:Glioblastoma / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D006801:Humans; D035683:MicroRNAs / Q000737:chemistry / Q000235:genetics*; D034622:RNA Interference; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics; D056564:Sirtuin 1 / Q000737:chemistry / Q000235:genetics*; D059467:Transcriptome
chemical_list: D020413:3' Untranslated Regions; C548375:MIRN22 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; D056564:Sirtuin 1
keywords: Glioblastoma; SIRT1; miR-22