title: Effect of controlled release of brain-derived neurotrophic factor and neurotrophin-3 from collagen gel on neural stem cells.
abstract: This study aimed to examine the effect of controlled release of brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3) from collagen gel on rat neural stem cells (NSCs). With three groups of collagen gel, BDNF/collagen gel, and NT-3/collagen gel as controls, BDNF and NT-3 were tested in the BDNF-NT-3/collagen gel group at different time points. The enzyme-linked immunosorbent assay results showed that BDNF and NT-3 were steadily released from collagen gels for 10 days. The cell viability test and the bromodeoxyuridine incorporation assay showed that BDNF-NT-3/collagen gel supported the survival and proliferation of NSCs. The results also showed that the length of processes was markedly longer and differentiation percentage from NSCs into neurons was much higher in the BDNF-NT-3/collagen gel group than those in the collagen gel, BDNF/collagen gel, and NT-3/collagen gel groups. These findings suggest that BDNF-NT-3/collagen gel could significantly improve the ability of NSCs proliferation and differentiation.
authors.affiliation: aDepartment of Orthopaedics, The First Affiliated Hospital of Anhui Medical University bDepartment of Orthopaedics, The Fourth Affiliated Hospital of Anhui Medical University, Hefei, Anhui, People' s Republic of China.
authors.forename: Fei
authors.initials: F
authors.lastname: Huang
authors.forename: Yunfeng
authors.initials: Y
authors.lastname: Wu
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
authors.forename: Jun
authors.initials: J
authors.lastname: Chang
authors.forename: Guangwen
authors.initials: G
authors.lastname: Ma
authors.forename: Zongsheng
authors.initials: Z
authors.lastname: Yin
mesh_terms: D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000493:pharmacokinetics / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000494:pharmacology*; D005260:Female; D058953:Neural Stem Cells / Q000187:drug effects*; D020933:Neurotrophin 3 / Q000493:pharmacokinetics / Q000494:pharmacology*; D010641:Phenotype; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D020933:Neurotrophin 3; C579455:BDNF protein, human; D003094:Collagen