title: Microbial Transformation of 14-Anhydrodigoxigenin by Alternaria alternata.
abstract: The microbial transformation of 14-anhydrodigoxigenin (1) by Alternaria alternata CGMCC 3.577 led to the production of seven new metabolites, 2-8. Their structures were determined by extensive spectroscopic (CD, IR, 1D- and 2D-NMR, and HR-ESI-MS) data analyses. The reactions in the bioprocess exhibited diversity, including specific oxidation, hydroxylation, reduction, epoxidation, and dehydration. In addition, a hypothetical biocatalytic pathway is proposed.
authors.affiliation: State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Peking Union Medical College and Chinese Academy of Medical Sciences, 1 Xian Nong Tan Street, Beijing 100050, P. R. China, (phone: +86-10-63165195; fax: +86-10-63017757).|Beijing Key Laboratory of Non-Clinical Drug Metabolism and PK/PD Study, Institute of Materia Medica, Peking Union Medical College and Chinese Academy of Medical Sciences, 1 Xian Nong Tan Street, Beijing 100050, P. R. China.
authors.forename: Jimei
authors.initials: J
authors.lastname: Liu
authors.affiliation: Institute of Chemistry and Chemistry Engineering, Qiqihar University, Qiqihar 161006, P. R. China.
authors.forename: Wanxia
authors.initials: W
authors.lastname: Tang
authors.affiliation: State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Peking Union Medical College and Chinese Academy of Medical Sciences, 1 Xian Nong Tan Street, Beijing 100050, P. R. China, (phone: +86-10-63165195; fax: +86-10-63017757).|Beijing Key Laboratory of Non-Clinical Drug Metabolism and PK/PD Study, Institute of Materia Medica, Peking Union Medical College and Chinese Academy of Medical Sciences, 1 Xian Nong Tan Street, Beijing 100050, P. R. China.
authors.forename: Ridao
authors.initials: R
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Peking Union Medical College and Chinese Academy of Medical Sciences, 1 Xian Nong Tan Street, Beijing 100050, P. R. China, (phone: +86-10-63165195; fax: +86-10-63017757). jgdai@imm.ac.cn.|Beijing Key Laboratory of Non-Clinical Drug Metabolism and PK/PD Study, Institute of Materia Medica, Peking Union Medical College and Chinese Academy of Medical Sciences, 1 Xian Nong Tan Street, Beijing 100050, P. R. China. jgdai@imm.ac.cn.
authors.forename: Jungui
authors.initials: J
authors.lastname: Dai
mesh_terms: D065767:Activation, Metabolic; D000528:Alternaria / Q000737:chemistry / Q000378:metabolism*; D004076:Digoxigenin / Q000031:analogs & derivatives* / Q000737:chemistry*; D006900:Hydroxylation; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure
chemical_list: C000610654:14-anhydrodigoxigenin; D004076:Digoxigenin
keywords: 14-Anhydrodigoxigenin; Alternaria alternata; Cardenolides; Microbial transformation