title: Study of enteropathogenic bacteria in children with acute diarrhoea aged from 7 to 10 years in Xuzhou, China.
abstract: BACKGROUND
  Acute diarrhoea is a common infectious disease among children in many countries and it has different kinds of clinical symptoms including vomiting, abdominal cramps, or fever of 38 °C. Some specific intestinal bacteria and their quantities can result in relevant symptoms.
  
  
  OBJECTIVE
  To analyze the correspondence between enteropathogenic bacteria and acute diarrhoea at family-level using high-throughput sequencing approach.
  
  
  METHODS
  Every 30 children of acute diarrhoea with abdominal cramps, vomiting, and fever of 38 °C was regarded as a group, respectively. Stools samples were collected from each group and the DNA of stool was examined by E.Z.N.A.(®) Stool DNA Kit. The 16S rRNA genes sequencing was performed on an Illumina Miseq platform.
  
  
  RESULTS
  The sequencing dataset comprised 65,092 valid reads sequences that affiliated to the 18 phylogenetic families. The four dominant taxonomic groups in all three samples were Streptococcaceae, Veillonellaceae, Enterobacteriaceae and Lactobacillaceae. The stools of children with high fever presented higher pathogenic bacterial diversities and more complex community structures than other two groups. Lactobacillaceae was the enteric predominant microflora that could reduce the severity of acute diarrhoea.
  
  
  CONCLUSIONS
  The reduction of predominant microflora or the aberrant proliferation of sub-dominant microflora can break the intestinal operation mechanism and cause intestinal diseases. What's more, people's living habits are also correlative about acute diarrhoea and parents should prepare light food for their children in order to protect their tender gastrointestinal mucosa.
authors.affiliation: School of Life Science, Jiangsu Normal University, Xuzhou, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Yin
authors.affiliation: School of Life Science, Jiangsu Normal University, Xuzhou, China.
authors.forename: Xiaohe
authors.initials: X
authors.lastname: Gu
authors.affiliation: School of Life Science, Jiangsu Normal University, Xuzhou, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Yin
authors.affiliation: School of Life Science, Jiangsu Normal University, Xuzhou, China. Electronic address: wenhy@jsnu.edu.cn.
authors.forename: Hongyu
authors.initials: H
authors.lastname: Wen
authors.affiliation: School of Life Science, Jiangsu Normal University, Xuzhou, China.
authors.forename: Xiali
authors.initials: X
authors.lastname: Gao
authors.affiliation: School of Life Science, Jiangsu Normal University, Xuzhou, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Zheng
mesh_terms: D000208:Acute Disease; D002648:Child; D002681:China / Q000453:epidemiology; D003967:Diarrhea / Q000453:epidemiology / Q000382:microbiology*; D004755:Enterobacteriaceae / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D005243:Feces / Q000382:microbiology; D005260:Female; D006801:Humans; D008297:Male; D010802:Phylogeny; D013289:Streptococcaceae / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*
keywords: Acute diarrhoea; Enteropathogenic bacteria; High-throughput sequencing