title: Application of Proteomics in Cancer Biomarker Discovery: GeLC-MS/MS.
abstract: Proteomic approaches are being increasingly applied to study multiple facets of healthy and diseased processes. In particular, the application of global proteome profiling in the field of oncology is already starting to shape the diagnostic, prognostic, monitoring, and therapeutic approaches. At the heart of such approaches lies a quest for clinically relevant biomarkers, particularly arising from global analyses of body fluids, as, in major part, they represent easily accessible and noninvasive matrices. A detailed protocol of one of the popular approaches for global proteome profiling, SDS-PAGE-liquid chromatography-tandem mass spectrometry or GeLC-MS/MS, and its application for biomarker discovery in urine is provided here.
authors.affiliation: Centre for Haemato-Oncology, Bart Cancer Institute, Queen Mary University of London, 3rd Floor, John Vane Science Centre, John Vane Science Building, Charterhouse Square, London, EC1 6BQ, UK. p.cutillas@qmul.ac.uk.
authors.forename: Pedro R
authors.initials: PR
authors.lastname: Cutillas
authors.affiliation: Molecular Oncology Centre, Bart Cancer Institute, Queen Mary University of London, John Vane Science Building, Charterhouse Square, London, EC1 6BQ, UK.
authors.forename: Tatjana
authors.initials: T
authors.lastname: Crnogorac-Jurcevic
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis / Q000652:urine*; D011232:Chemical Precipitation; D002853:Chromatography, Liquid / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006801:Humans; D009369:Neoplasms / Q000175:diagnosis / Q000652:urine*; D011507:Proteinuria / Q000175:diagnosis / Q000652:urine*; D040901:Proteomics / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D014408:Biomarkers, Tumor
keywords: Biomarker discovery; Body fluids; GeLC-MS/MS; Proteomics