title: Correlative cryo-fluorescence and cryo-scanning electron microscopy as a straightforward tool to study host-pathogen interactions.
abstract: Correlative light and electron microscopy is an imaging technique that enables identification and targeting of fluorescently tagged structures with subsequent imaging at near-to-nanometer resolution. We established a novel correlative cryo-fluorescence microscopy and cryo-scanning electron microscopy workflow, which enables imaging of the studied object of interest very close to its natural state, devoid of artifacts caused for instance by slow chemical fixation. This system was tested by investigating the interaction of the zoonotic bacterium Borrelia burgdorferi with two mammalian cell lines of neural origin in order to broaden our knowledge about the cell-association mechanisms that precedes the entry of the bacteria into the cell. This method appears to be an unprecedentedly fast (<3 hours), straightforward, and reliable solution to study the finer details of pathogen-host cell interactions and provides important insights into the complex and dynamic relationship between a pathogen and a host.
authors.affiliation: Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, Branišovská 31, České Budějovice CZ-37005, Czech Republic.|Faculty of Science, University of South Bohemia in České Budějovice, Branišovská 1760, České Budějovice CZ-37005, Czech Republic.
authors.forename: Martin
authors.initials: M
authors.lastname: Strnad
authors.affiliation: Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, Branišovská 31, České Budějovice CZ-37005, Czech Republic.|Faculty of Science, University of South Bohemia in České Budějovice, Branišovská 1760, České Budějovice CZ-37005, Czech Republic.|Department of Virology, Veterinary Research Institute, Brno CZ-62100, Czech Republic.
authors.forename: Jana
authors.initials: J
authors.lastname: Elsterová
authors.affiliation: Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, Branišovská 31, České Budějovice CZ-37005, Czech Republic.|Faculty of Science, University of South Bohemia in České Budějovice, Branišovská 1760, České Budějovice CZ-37005, Czech Republic.
authors.forename: Jana
authors.initials: J
authors.lastname: Schrenková
authors.affiliation: Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, Branišovská 31, České Budějovice CZ-37005, Czech Republic.|Faculty of Science, University of South Bohemia in České Budějovice, Branišovská 1760, České Budějovice CZ-37005, Czech Republic.
authors.forename: Marie
authors.initials: M
authors.lastname: Vancová
authors.affiliation: Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, Branišovská 31, České Budějovice CZ-37005, Czech Republic.
authors.forename: Ryan O M
authors.initials: RO
authors.lastname: Rego
authors.affiliation: Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, Branišovská 31, České Budějovice CZ-37005, Czech Republic.|Faculty of Science, University of South Bohemia in České Budějovice, Branišovská 1760, České Budějovice CZ-37005, Czech Republic.
authors.forename: Libor
authors.initials: L
authors.lastname: Grubhoffer
authors.affiliation: Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, Branišovská 31, České Budějovice CZ-37005, Czech Republic.|Faculty of Science, University of South Bohemia in České Budějovice, Branišovská 1760, České Budějovice CZ-37005, Czech Republic.|Faculty of Science, Charles University in Prague, Viničná 1594/7, Praha CZ-12800, Czech Republic.
authors.forename: Jana
authors.initials: J
authors.lastname: Nebesářová
mesh_terms: D025065:Borrelia burgdorferi / Q000472:pathogenicity*; D002460:Cell Line; D020285:Cryoelectron Microscopy / Q000379:methods; D005453:Fluorescence; D054884:Host-Pathogen Interactions / Q000502:physiology*; D006801:Humans; D008855:Microscopy, Electron, Scanning / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods