title: Effect of Sardina pilchardus oil on alloxan-induced diabetic rats.
abstract: The purpose of this study was to prevent or to delay the onset of diabetes-related complications, by using a natural marine resource, Sardina pilchardus oil, administrated to alloxan-induced diabetic rats showing hyperglycemia and hyperlipidemia. Gas chromatography-mass spectrometry analysis of the sardine oil detected 18 constituents. The major ones were n-3 highly unsaturated fatty acids among which are docosahexaenoic acid (25.09%) and eicosapentaenoic acid (19.61%). Sardine oil inhibited the α-amylase activity in rats' sera (26.82%) and thus improved glycemia (54%). The supplement of this oil protected the β-cells from death and damage, significantly decreased total triglycerides, total cholesterol and LDL-cholesterol concentrations in diabetic rats' sera and increased the HDL-cholesterol level. Gavage administration of this oil to rats protected the liver and kidney functions by reducing the aspartate transaminase, alanine transaminase and phosphatase alkaline activities, and by decreasing creatinine, urea and uric acid levels.
authors.affiliation: a Laboratory of Animal Ecophysiology , Faculty of Sciences of Sfax, University of Sfax , Sfax , Tunisia .
authors.forename: Sahla
authors.initials: S
authors.lastname: BelHadj
authors.affiliation: b High School of Biotechnology of Sfax (ISBS), University of Sfax , Sfax , Tunisia .
authors.forename: Olfa
authors.initials: O
authors.lastname: Hentati
authors.affiliation: c Laboratory of Chemistry of Natural Products , Faculty of Sciences of Sfax, University of Sfax , Sfax , Tunisia , and.
authors.forename: Noura
authors.initials: N
authors.lastname: Baccouch
authors.affiliation: c Laboratory of Chemistry of Natural Products , Faculty of Sciences of Sfax, University of Sfax , Sfax , Tunisia , and.
authors.forename: Hichem
authors.initials: H
authors.lastname: Ben Salah
authors.affiliation: d Department of Pathology , Habib Bourguiba Hospital , Sfax , Tunisia.
authors.forename: Tahia
authors.initials: T
authors.lastname: Boudaouara
authors.affiliation: d Department of Pathology , Habib Bourguiba Hospital , Sfax , Tunisia.
authors.forename: Ayda
authors.initials: A
authors.lastname: Ben Hadj
authors.affiliation: c Laboratory of Chemistry of Natural Products , Faculty of Sciences of Sfax, University of Sfax , Sfax , Tunisia , and.
authors.forename: Noureddine
authors.initials: N
authors.lastname: Allouch
authors.affiliation: a Laboratory of Animal Ecophysiology , Faculty of Sciences of Sfax, University of Sfax , Sfax , Tunisia .
authors.forename: Abdel Fattah
authors.initials: AF
authors.lastname: El Feki
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy* / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D005395:Fish Oils / Q000494:pharmacology* / Q000627:therapeutic use; D050417:Insulin-Secreting Cells / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D007668:Kidney / Q000187:drug effects / Q000378:metabolism / Q000503:physiopathology; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D051381:Rats; D014280:Triglycerides / Q000097:blood; D000516:alpha-Amylases / Q000378:metabolism
chemical_list: D001786:Blood Glucose; D005395:Fish Oils; D014280:Triglycerides; D000516:alpha-Amylases
keywords: Sardine oil; glycemia; lipid profile; n-3 PUFAs; pancreatic α-amylase