title: [Cloning, expression, purification and characterization of two uracil-DNA glycosylases from Sulfolobus acidocaldarius].
abstract: OBJECTIVE
  To characterize uracil-DNA glycosylase from acidophilic and thermophilic Sulfolobus acidocaldarius.
  
  
  METHODS
  We cloned udgIV and udgV genes from S. acidocaldarius, expressed the two recombinant UDG proteins in E. coli species BL21 (DE3) Rosetta-pLysS, purified the recombinant UDGs and characterized the removal of dU by UDGs.
  
  
  RESULTS
  We successfully expressed two S. acidocaldarius UDGs and found both UDGs having the activity of dU removal. In comparison to UDGV, UDGIV was more efficient in dU removal, with a 750-foldactivity.
  
  
  CONCLUSIONS
  In comparison to UDGV, UDGIV from S. acidocaldarius was a more efficient enzyme responsible for the removal of dU from DNA in vitro.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Gangshun
authors.initials: G
authors.lastname: Yi
authors.forename: Jie
authors.initials: J
authors.lastname: Ou
authors.forename: Jianhua
authors.initials: J
authors.lastname: Liu
authors.forename: Xipeng
authors.initials: X
authors.lastname: Liu
mesh_terms: D019843:Archaeal Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D003001:Cloning, Molecular; D004795:Enzyme Stability; D007700:Kinetics; D016945:Sulfolobus acidocaldarius / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics*; D014498:Uracil / Q000378:metabolism; D051981:Uracil-DNA Glycosidase / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D019843:Archaeal Proteins; D014498:Uracil; D051981:Uracil-DNA Glycosidase