title: In Situ Sodium Bisulfite Modification of Genomic DNA from Microdissected Melanoma Paraffin-Embedded Archival Tissues.
abstract: DNA methylation analysis of paraffin-embedded archival tumor tissues (PEAT) is important in clinical and translational research studies. Efficient identification and isolation of homogeneous cell population, optimal DNA extraction, and sodium bisulfite modification (SBM) are essential, particularly in small tumor lesions. Laser capture microdissection (LCM) coupled with an in situ SBM improves the specificity, through histopathology accuracy, and the amount of genomic DNA modified for downstream methylation assays.
authors.affiliation: Department of Molecular Oncology, John Wayne Cancer Institute, Providence Saint John's Health Center, Santa Monica, CA, 90404, USA.
authors.forename: Diego M
authors.initials: DM
authors.lastname: Marzese
authors.affiliation: Department of Molecular Oncology, John Wayne Cancer Institute, Providence Saint John's Health Center, Santa Monica, CA, 90404, USA.
authors.forename: Sharon K
authors.initials: SK
authors.lastname: Huang
authors.affiliation: Department of Molecular Oncology, John Wayne Cancer Institute, Providence Saint John's Health Center, Santa Monica, CA, 90404, USA. hoon@jwci.org.
authors.forename: Dave S B
authors.initials: DS
authors.lastname: Hoon
keywords: DNA methylation; Laser capture microdissection (LCM); Melanoma; Sodium bisulfite modification (SBM)