title: Longitudinal evaluation of diagnostics in experimentally infected young calves during subclinical and clinical paratuberculosis.
abstract: Five calves were inoculated orally at 2 weeks of age with a dose of 5 × 10(9) colony-forming units of Mycobacterium avium subspecies paratuberculosis (MAP) on 2 consecutive days. Two calves developed clinical Johne's disease at 12 and 16 months of age after being consistently positive for MAP on fecal culture and antibody enzyme-linked immunosorbent assay (ELISA), starting 2 to 3 weeks and 4 to 5 months after inoculation, respectively.
authors.affiliation: Department of Production Animal Health (Mortier, Barkema, Orsel, De Buck), Veterinary Science Research Station (Muench), Department of Ecosystem and Public Health (Bystrom), Faculty of Veterinary Medicine, University of Calgary, Calgary, Alberta; Department of Biomedical Sciences, School of Veterinary Medicine, Ross University, Basseterre, Saint Kitts, West Indies (Illanes).
authors.forename: Rienske A R
authors.initials: RA
authors.lastname: Mortier
authors.affiliation: Department of Production Animal Health (Mortier, Barkema, Orsel, De Buck), Veterinary Science Research Station (Muench), Department of Ecosystem and Public Health (Bystrom), Faculty of Veterinary Medicine, University of Calgary, Calgary, Alberta; Department of Biomedical Sciences, School of Veterinary Medicine, Ross University, Basseterre, Saint Kitts, West Indies (Illanes).
authors.forename: Herman W
authors.initials: HW
authors.lastname: Barkema
authors.affiliation: Department of Production Animal Health (Mortier, Barkema, Orsel, De Buck), Veterinary Science Research Station (Muench), Department of Ecosystem and Public Health (Bystrom), Faculty of Veterinary Medicine, University of Calgary, Calgary, Alberta; Department of Biomedical Sciences, School of Veterinary Medicine, Ross University, Basseterre, Saint Kitts, West Indies (Illanes).
authors.forename: Karin
authors.initials: K
authors.lastname: Orsel
authors.affiliation: Department of Production Animal Health (Mortier, Barkema, Orsel, De Buck), Veterinary Science Research Station (Muench), Department of Ecosystem and Public Health (Bystrom), Faculty of Veterinary Medicine, University of Calgary, Calgary, Alberta; Department of Biomedical Sciences, School of Veterinary Medicine, Ross University, Basseterre, Saint Kitts, West Indies (Illanes).
authors.forename: Gregory P
authors.initials: GP
authors.lastname: Muench
authors.affiliation: Department of Production Animal Health (Mortier, Barkema, Orsel, De Buck), Veterinary Science Research Station (Muench), Department of Ecosystem and Public Health (Bystrom), Faculty of Veterinary Medicine, University of Calgary, Calgary, Alberta; Department of Biomedical Sciences, School of Veterinary Medicine, Ross University, Basseterre, Saint Kitts, West Indies (Illanes).
authors.forename: Janet M
authors.initials: JM
authors.lastname: Bystrom
authors.affiliation: Department of Production Animal Health (Mortier, Barkema, Orsel, De Buck), Veterinary Science Research Station (Muench), Department of Ecosystem and Public Health (Bystrom), Faculty of Veterinary Medicine, University of Calgary, Calgary, Alberta; Department of Biomedical Sciences, School of Veterinary Medicine, Ross University, Basseterre, Saint Kitts, West Indies (Illanes).
authors.forename: Oscar
authors.initials: O
authors.lastname: Illanes
authors.affiliation: Department of Production Animal Health (Mortier, Barkema, Orsel, De Buck), Veterinary Science Research Station (Muench), Department of Ecosystem and Public Health (Bystrom), Faculty of Veterinary Medicine, University of Calgary, Calgary, Alberta; Department of Biomedical Sciences, School of Veterinary Medicine, Ross University, Basseterre, Saint Kitts, West Indies (Illanes).
authors.forename: Jeroen
authors.initials: J
authors.lastname: De Buck
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000473:pathology; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005243:Feces / Q000382:microbiology; D008137:Longitudinal Studies; D008297:Male; D016927:Mycobacterium avium subsp. paratuberculosis / Q000276:immunology / Q000302:isolation & purification; D010283:Paratuberculosis / Q000175:diagnosis* / Q000473:pathology; D013997:Time Factors
chemical_list: D000907:Antibodies, Bacterial