title: FT-IR spectroscopy: A powerful tool for studying the inter- and intraspecific biodiversity of cultivable non-Saccharomyces yeasts isolated from grape must.
abstract: The efficiency of the FT-IR technique for studying the inter- and intra biodiversity of cultivable non-Saccharomyces yeasts (NS) present in different must samples was examined. In first, the capacity of the technique FT-IR to study the global diversity of a given sample was compared to the pyrosequencing method, used as a reference technique. Seven different genera (Aureobasidium, Candida, Cryptococcus, Hanseniaspora, Issatchenkia, Metschnikowia and Pichia) were identified by FT-IR and also by pyrosequencing. Thirty-eight other genera were identified by pyrosequencing, but together they represented less than 6% of the average total population of 6 musts. Among the species identified, some of them present organoleptic potentials in winemaking, particularly Starmerella bacillaris (synonym Candidazemplinina). So in a second time, we evaluated the capacity of the FT-IR technique to discriminate the isolates of this species because few techniques were able to study intraspecific NS yeast biodiversity. The results obtained were validated by using a classic method as ITS sequencing. Biodiversity at strain level was high: 19 different strains were identified from 58 isolates. So, FT-IR spectroscopy seems to be an accurate and reliable method for identifying major genera present in the musts. The two biggest advantages of the FT-IR are the capacity to characterize intraspecific biodiversity of non-Saccharomyces yeasts and the possibility to discriminate a lot of strains.
authors.affiliation: UMR Procédés Alimentaires et Microbiologiques, Equipe VAlMiS (Vin, Aliment, Microbiologie, Stress), AgroSup Dijon - Université de Bourgogne/Franche-Comté, IUVV, Rue Claude Ladrey, BP 27877, 21000 Dijon, France.
authors.forename: Cédric
authors.initials: C
authors.lastname: Grangeteau
authors.affiliation: Institut für Mikrobiologie und Biochemie Zentrum Analytische Chemie und Mikrobiologie - Hochschule Geisenheim University, Geisenheim, Germany.
authors.forename: Daniel
authors.initials: D
authors.lastname: Gerhards
authors.affiliation: INRA, UMR 1347 Agroécologie-Plateforme Genosol, 17, rue Sully, BP 86510, 21000 Dijon, France.
authors.forename: Sebastien
authors.initials: S
authors.lastname: Terrat
authors.affiliation: INRA, UMR 1347 Agroécologie-Plateforme Genosol, 17, rue Sully, BP 86510, 21000 Dijon, France.
authors.forename: Samuel
authors.initials: S
authors.lastname: Dequiedt
authors.affiliation: UMR Procédés Alimentaires et Microbiologiques, Equipe VAlMiS (Vin, Aliment, Microbiologie, Stress), AgroSup Dijon - Université de Bourgogne/Franche-Comté, IUVV, Rue Claude Ladrey, BP 27877, 21000 Dijon, France.
authors.forename: Hervé
authors.initials: H
authors.lastname: Alexandre
authors.affiliation: UMR Procédés Alimentaires et Microbiologiques, Equipe VAlMiS (Vin, Aliment, Microbiologie, Stress), AgroSup Dijon - Université de Bourgogne/Franche-Comté, IUVV, Rue Claude Ladrey, BP 27877, 21000 Dijon, France.
authors.forename: Michèle
authors.initials: M
authors.lastname: Guilloux-Benatier
authors.affiliation: Institut für Mikrobiologie und Biochemie Zentrum Analytische Chemie und Mikrobiologie - Hochschule Geisenheim University, Geisenheim, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: von Wallbrunn
authors.affiliation: UMR Procédés Alimentaires et Microbiologiques, Equipe VAlMiS (Vin, Aliment, Microbiologie, Stress), AgroSup Dijon - Université de Bourgogne/Franche-Comté, IUVV, Rue Claude Ladrey, BP 27877, 21000 Dijon, France. Electronic address: sandrine.rousseaux@u-bourgogne.fr.
authors.forename: Sandrine
authors.initials: S
authors.lastname: Rousseaux
mesh_terms: D001483:Base Sequence; D044822:Biodiversity; D019295:Computational Biology; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D016533:Mycological Typing Techniques / Q000379:methods*; D010802:Phylogeny; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D012440:Saccharomyces / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D017422:Sequence Analysis, DNA; D017550:Spectroscopy, Fourier Transform Infrared / Q000295:instrumentation / Q000379:methods*; D027843:Vitis / Q000382:microbiology*; D014920:Wine / Q000382:microbiology; D015003:Yeasts / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D004271:DNA, Fungal; D012337:RNA, Ribosomal, 18S
keywords: Candida; FT-IR spectroscopy; Pyrosequencing; Strain typing; Wine yeast