title: Anti-Obesity Effect of 6,8-Diprenylgenistein, an Isoflavonoid of Cudrania tricuspidata Fruits in High-Fat Diet-Induced Obese Mice.
abstract: Obesity, which is characterized by excessive fat accumulation, is associated with several pathological disorders, including metabolic diseases. In this study, the anti-obesity effect of 6,8-diprenylgenistein (DPG), a major isoflavonoid of Cudrania tricuspidata fruits was investigated using high fat-diet (HFD)-induced obese mice at the doses of 10 and 30 mg/kg for six week. The body weight of the DPG-treated groups was significantly lower compared to the HFD-treated group. In addition, fat accumulation in epididymal adipose tissue and liver was dramatically decreased in the HFD + DPG groups. The food efficiency ratios of the HFD + DPG groups were also lower compared to the HFD group with the same food intake. Metabolic parameters that had increased in the HFD group were decreased in the HFD + DPG groups. Further studies demonstrate that DPG efficiently reduces lipogenic genes by regulation of transcription factors, such as peroxisome proliferator-activated receptor γ (PPARγ) and CCAAT/enhancer-binding protein α (C/EBPα), and hormones, such as leptin and adiponection. DPG also regulates acetyl-CoA carboxylase (ACC) and hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR) by AMP-activated protein kinase (AMPK) activation. Taken together, DPG is beneficial for the regulation of obesity, especially resulting from high fat intake.
authors.affiliation: College of Pharmacy, Chungbuk National University, Cheongju, Chungbuk 28644, Korea. qow0125@naver.com.
authors.forename: Yang Hee
authors.initials: YH
authors.lastname: Jo
authors.affiliation: College of Pharmacy, Chungbuk National University, Cheongju, Chungbuk 28644, Korea. mirine0101@hanmail.net.
authors.forename: Kyeong-Mi
authors.initials: KM
authors.lastname: Choi
authors.affiliation: College of Pharmacy, Chungbuk National University, Cheongju, Chungbuk 28644, Korea. liuqing7115@hotmail.com.
authors.forename: Qing
authors.initials: Q
authors.lastname: Liu
authors.affiliation: College of Pharmacy, Chungbuk National University, Cheongju, Chungbuk 28644, Korea. suntiger85@hanmail.net.
authors.forename: Seon Beom
authors.initials: SB
authors.lastname: Kim
authors.affiliation: Laboratory Animal Research Center, Chungbuk National University, Cheongju, Chungbuk 28644, Korea. jihjin@chungbuk.ac.kr.
authors.forename: Hyeong-Jin
authors.initials: HJ
authors.lastname: Ji
authors.affiliation: Laboratory Animal Research Center, Chungbuk National University, Cheongju, Chungbuk 28644, Korea. mhkim3340@naver.com.
authors.forename: Myounghwan
authors.initials: M
authors.lastname: Kim
authors.affiliation: Laboratory Animal Research Center, Chungbuk National University, Cheongju, Chungbuk 28644, Korea. skshin@chungbuk.ac.kr.
authors.forename: Sang-Kyung
authors.initials: SK
authors.lastname: Shin
authors.affiliation: Wellness R&amp;D Center, Univera, Inc., Seoul 05014, Korea. sgildo@univera.com.
authors.forename: Seon-Gil
authors.initials: SG
authors.lastname: Do
authors.affiliation: Wellness R&amp;D Center, Univera, Inc., Seoul 05014, Korea. Ejayshin@univera.com.
authors.forename: Eunju
authors.initials: E
authors.lastname: Shin
authors.affiliation: Wellness R&amp;D Center, Univera, Inc., Seoul 05014, Korea. gayoung@univera.com.
authors.forename: Gayoung
authors.initials: G
authors.lastname: Jung
authors.affiliation: College of Pharmacy, Chungbuk National University, Cheongju, Chungbuk 28644, Korea. yoohs@chungbuk.ac.kr.
authors.forename: Hwan-Soo
authors.initials: HS
authors.lastname: Yoo
authors.affiliation: College of Pharmacy, Chungbuk National University, Cheongju, Chungbuk 28644, Korea. byhwang@chungbuk.ac.kr.
authors.forename: Bang Yeon
authors.initials: BY
authors.lastname: Hwang
authors.affiliation: College of Pharmacy, Chungbuk National University, Cheongju, Chungbuk 28644, Korea. mklee@chungbuk.ac.kr.
authors.forename: Mi Kyeong
authors.initials: MK
authors.lastname: Lee
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism; D000103:Acetyl-CoA Carboxylase / Q000235:genetics / Q000378:metabolism; D052242:Adiponectin / Q000378:metabolism; D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D019440:Anti-Obesity Agents / Q000494:pharmacology*; D022763:CCAAT-Enhancer-Binding Protein-alpha / Q000235:genetics / Q000378:metabolism; D059305:Diet, High-Fat; D005638:Fruit / Q000737:chemistry*; D005786:Gene Expression Regulation; D019833:Genistein / Q000031:analogs & derivatives* / Q000494:pharmacology; D006903:Hydroxymethylglutaryl CoA Reductases / Q000235:genetics / Q000378:metabolism; D007529:Isoflavones / Q000494:pharmacology*; D020738:Leptin / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008820:Mice, Obese; D029586:Moraceae / Q000737:chemistry*; D009765:Obesity / Q000188:drug therapy*; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology
chemical_list: C401294:6,8-diprenylgenistein; D052242:Adiponectin; D019440:Anti-Obesity Agents; D022763:CCAAT-Enhancer-Binding Protein-alpha; D007529:Isoflavones; D020738:Leptin; D047495:PPAR gamma; D010936:Plant Extracts; D019833:Genistein; D006903:Hydroxymethylglutaryl CoA Reductases; D055372:AMP-Activated Protein Kinases; D000103:Acetyl-CoA Carboxylase
keywords: 6,8-Diprenylgenistein (DPG); AMPK; Cudrania tricuspidata; high-fat diet-induced obesity; lipid profile