title: Localization and quantification of intramuscular damage using statistical parametric mapping and skeletal muscle parcellation.
abstract: In the present study, we proposed an original and robust methodology which combines the spatial normalization of skeletal muscle images, the statistical parametric mapping (SPM) analysis and the use of a specific parcellation in order to accurately localize and quantify the extent of skeletal muscle damage within the four heads of the quadriceps femoris. T2 maps of thigh muscles were characterized before, two (D2) and four (D4) days after 40 maximal isometric electrically-evoked contractions in 25 healthy young males. On the basis of SPM analysis of coregistrated T2 maps, the alterations were similarly detected at D2 and D4 in the superficial and distal regions of the vastus medialis (VM) whereas the proportion of altered muscle was higher in deep muscle regions of the vastus lateralis at D4 (deep: 35 ± 25%, superficial: 23 ± 15%) as compared to D2 (deep: 18 ± 13%, superficial: 17 ± 13%). The present methodology used for the first time on skeletal muscle would be of utmost interest to detect subtle intramuscular alterations not only for the diagnosis of muscular diseases but also for assessing the efficacy of potential therapeutic interventions and clinical treatment strategies.
authors.affiliation: Aix-Marseille Université, CNRS, CRMBM, UMR 7339, 13385, Marseille, France.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Fouré
authors.affiliation: Aix-Marseille Université, CNRS, CRMBM, UMR 7339, 13385, Marseille, France.
authors.forename: Arnaud
authors.initials: A
authors.lastname: Le Troter
authors.affiliation: Aix-Marseille Université, CNRS, CRMBM, UMR 7339, 13385, Marseille, France.|APHM, Hôpital de la Timone, CEMEREM, Pôle Imagerie Médicale, 13005, Marseille, France.
authors.forename: Maxime
authors.initials: M
authors.lastname: Guye
authors.affiliation: Aix-Marseille Université, CNRS, CRMBM, UMR 7339, 13385, Marseille, France.|APHM, Hôpital de Sainte Marguerite, Service de Rhumatologie, Pôle Appareil Locomoteur, 13005, Marseille, France.
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Mattei
authors.affiliation: Aix-Marseille Université, CNRS, CRMBM, UMR 7339, 13385, Marseille, France.
authors.forename: David
authors.initials: D
authors.lastname: Bendahan
authors.affiliation: Aix-Marseille Université, CNRS, CRMBM, UMR 7339, 13385, Marseille, France.
authors.forename: Julien
authors.initials: J
authors.lastname: Gondin
mesh_terms: D004566:Electrodes; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D018482:Muscle, Skeletal / Q000473:pathology*; D012984:Software; D013223:Statistics as Topic; D055815:Young Adult