title: Soluble Moringa oleifera leaf extract reduces intracellular cadmium accumulation and oxidative stress in Saccharomyces cerevisiae.
abstract: Moringa oleifera leaves are a well-known source of antioxidants and traditionally used for medicinal applications. In the present study, the protective action of soluble M. oleifera leaf extract (MOLE) against cadmium toxicity was investigated in the model eukaryote Saccharomyces cerevisiae. The results showed that this extract exhibited a protective effect against oxidative stress induced by cadmium and H2O2 through the reduction of intracellular reactive oxygen species. Interestingly, not only the co-exposure of soluble MOLE with cadmium but also pretreatment of this extract prior to cadmium exposure significantly reduced the cadmium uptake through an inhibition of Fet4p, a low-affinity iron(II) transporter. In addition, the supplementation of soluble MOLE significantly reduced intracellular iron accumulation in a Fet4p-independent manner. Our findings suggest the potential use of soluble extract from M. oleifera leaves as a dietary supplement for protection against cadmium accumulation and oxidative stress.
authors.affiliation: Department of Biology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand; Center of Excellence on Environmental Health and Toxicology, CHE, Ministry of Education, Thailand.
authors.forename: Kittikhun
authors.initials: K
authors.lastname: Kerdsomboon
authors.affiliation: Department of Biology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand; Center of Excellence on Environmental Health and Toxicology, CHE, Ministry of Education, Thailand.
authors.forename: Supinda
authors.initials: S
authors.lastname: Tatip
authors.affiliation: Department of Biology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.
authors.forename: Sattawat
authors.initials: S
authors.lastname: Kosasih
authors.affiliation: Department of Biology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand; Center of Excellence on Environmental Health and Toxicology, CHE, Ministry of Education, Thailand. Electronic address: choowong.aue@mahidol.ac.th.
authors.forename: Choowong
authors.initials: C
authors.lastname: Auesukaree
mesh_terms: D000975:Antioxidants / Q000494:pharmacology; D001692:Biological Transport / Q000187:drug effects; D002104:Cadmium / Q000378:metabolism*; D027682:Cation Transport Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism; D000079922:Copper Transport Proteins; D019587:Dietary Supplements; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007501:Iron / Q000378:metabolism; D033862:Iron-Binding Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism; D029588:Moringa oleifera / Q000737:chemistry*; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*; D017382:Reactive Oxygen Species / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism; D012995:Solubility
chemical_list: D000975:Antioxidants; D027682:Cation Transport Proteins; D000079922:Copper Transport Proteins; C105847:FET4 protein, S cerevisiae; D033862:Iron-Binding Proteins; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D029701:Saccharomyces cerevisiae Proteins; D002104:Cadmium; D006861:Hydrogen Peroxide; D007501:Iron
keywords: Cadmium; Iron; Moringa oleifera; Oxidative stress; Saccharomyces cerevisiae