title: Association of Methylenetetrahydrafolate Reductase Gene Polymorphism (MTHFR) in Patients with Gallbladder Cancer.
abstract: OBJECTIVE
  5,10-Methylenetetrahydrofolate reductase (MTHFR) is a key enzyme in folate metabolism and plays a major role in DNA methylation. There are two popular MTHFR polymorphisms known as C677T and A1298C which are found to be involved in folate metabolism and lowering the enzyme activity, thus may be linked with cancer development. This study aims to look at the association of these polymorphisms in gallbladder cancer.
  
  
  METHODS
  Thirty patients each with gallbladder cancer, cholelithiasis, and normal gallbladder were genotyped for the above-given polymorphisms by PCR-restriction fragment length polymorphism (RFLP) method.
  
  
  RESULTS
  C677T MTHFR polymorphism was not associated (χ(2) = 2.44, p = 0.85) with an increased likelihood of having gallbladder cancer. A1298C was significantly associated (χ(2) = 28.87, p < 0.001) with risk of developing gallbladder cancer. A1298C was significantly correlated with grade (r = 0.337, p < 0.001) and histopathology (r = 0.446, p < 0.001).
  
  
  CONCLUSIONS
  This study proposed that MTHFR A1298C polymorphism may be associated with risk of developing gallbladder cancer, and there is no association between C677T polymorphism and gallbladder cancer.
authors.affiliation: Department of General Surgery, Institute of Medical Sciences, Banaras Hindu University, Varanasi, 221 005, India.
authors.forename: Ruhi
authors.initials: R
authors.lastname: Dixit
authors.affiliation: Department of General Surgery, Institute of Medical Sciences, Banaras Hindu University, Varanasi, 221 005, India.
authors.forename: Gyanendra
authors.initials: G
authors.lastname: Singh
authors.affiliation: Department of Surgical Oncology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India.
authors.forename: Manoj
authors.initials: M
authors.lastname: Pandey
authors.affiliation: Department of General Surgery, Institute of Medical Sciences, Banaras Hindu University, Varanasi, 221 005, India.
authors.forename: Somprakas
authors.initials: S
authors.lastname: Basu
authors.affiliation: Department of General Surgery, Institute of Medical Sciences, Banaras Hindu University, Varanasi, 221 005, India.
authors.forename: Satyanam Kumar
authors.initials: SK
authors.lastname: Bhartiya
authors.affiliation: Department of Statistics, Faculty of Science, Banaras Hindu University, Varanasi, India.
authors.forename: K K
authors.initials: KK
authors.lastname: Singh
authors.affiliation: Department of General Surgery, Institute of Medical Sciences, Banaras Hindu University, Varanasi, 221 005, India. vkshuklabhu@gmail.com.
authors.forename: Vijay Kumar
authors.initials: VK
authors.lastname: Shukla
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D000328:Adult; D014408:Biomarkers, Tumor / Q000235:genetics*; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D016022:Case-Control Studies; D005260:Female; D005500:Follow-Up Studies; D005706:Gallbladder Neoplasms / Q000235:genetics* / Q000473:pathology; D006801:Humans; D008297:Male; D042965:Methylenetetrahydrofolate Reductase (NADPH2) / Q000235:genetics*; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis
chemical_list: D014408:Biomarkers, Tumor; C573423:MTHFR protein, human; D042965:Methylenetetrahydrofolate Reductase (NADPH2)
keywords: 5,10-Methylenetetrahydrofolate reductase (MTHFR); A1298C; C677T; Gallbladder cancer; Restriction fragment length polymorphism (RFLP)