title: Genetic characterisation of Taenia multiceps cysts from ruminants in Greece.
abstract: This study was designed to genetically characterise the larval stage (coenurus) of Taenia multiceps from ruminants in Greece, utilising DNA regions within the cytochrome c oxidase subunit 1 (partial cox1) and NADH dehydrogenase 1 (pnad1) mitochondrial (mt) genes, respectively. A molecular-phylogenetic approach was used to analyse the pcox1 and pnad1 amplicons derived from genomic DNA samples from individual cysts (n=105) from cattle (n=3), goats (n=5) and sheep (n=97). Results revealed five and six distinct electrophoretic profiles for pcox1 and pnad1, respectively, using single-strand conformation polymorphism. Direct sequencing of selected amplicons representing each of these profiles defined five haplotypes each for pcox1 and pnad1, among all 105 isolates. Phylogenetic analysis of individual sequence data for each locus, including a range of well-defined reference sequences, inferred that all isolates of T. multiceps cysts from ruminants in Greece clustered with previously published sequences from different continents. The present study provides a foundation for future large-scale studies on the epidemiology of T. multiceps in ruminants as well as dogs in Greece.
authors.affiliation: Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Werribee, Victoria 3030, Australia; Animal Health Research Centre, Ministry of Agriculture and Fisheries, Muscat, Oman.
authors.forename: Shumoos
authors.initials: S
authors.lastname: Al-Riyami
authors.affiliation: School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, University Campus, Thessaloniki, Greece.
authors.forename: Evi
authors.initials: E
authors.lastname: Ioannidou
authors.affiliation: Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Werribee, Victoria 3030, Australia.
authors.forename: Anson V
authors.initials: AV
authors.lastname: Koehler
authors.affiliation: Animal Health Research Centre, Ministry of Agriculture and Fisheries, Muscat, Oman.
authors.forename: Muhammad H
authors.initials: MH
authors.lastname: Hussain
authors.affiliation: Animal Health Research Centre, Ministry of Agriculture and Fisheries, Muscat, Oman.
authors.forename: Abdulmajeed H
authors.initials: AH
authors.lastname: Al-Rawahi
authors.affiliation: School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, University Campus, Thessaloniki, Greece.
authors.forename: Nektarios D
authors.initials: ND
authors.lastname: Giadinis
authors.affiliation: Department of Veterinary Pathology and Public Health, Faculty of Veterinary Medicine, Jordan University of Science and Technology, Irbid, Jordan.
authors.forename: Shawkat Q
authors.initials: SQ
authors.lastname: Lafi
authors.affiliation: School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, University Campus, Thessaloniki, Greece.
authors.forename: Elias
authors.initials: E
authors.lastname: Papadopoulos
authors.affiliation: Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Werribee, Victoria 3030, Australia. Electronic address: jabbara@unimelb.edu.au.
authors.forename: Abdul
authors.initials: A
authors.lastname: Jabbar
mesh_terms: D000818:Animals; D018509:DNA, Helminth; D006115:Greece; D006239:Haplotypes; D010802:Phylogeny; D018807:Polymorphism, Single-Stranded Conformational; D012418:Ruminants / Q000469:parasitology*; D013621:Taenia / Q000145:classification* / Q000235:genetics*
chemical_list: D018509:DNA, Helminth
keywords: Coenurus; Greece; PCR-SSCP; Ruminants; Taenia multiceps