title: Arsenic speciation and susceptibility to oxidative stress in the fanworm Sabella spallanzanii (Gmelin) (Annelida, Sabellidae) under naturally acidified conditions: An in situ transplant experiment in a Mediterranean CO2 vent system.
abstract: The fanworm Sabella spallanzanii (Gmelin, 1791) (Annelida, Sabellidae) is considered tolerant to several types of stressors but is generally absent from the CO2 vents. A peculiar characteristic of this species is the elevated content of arsenic in the gills, particularly dimethylarsinic acid (DMA), stored as an anti-predatory compound. In this study, modulation of trace metal levels, chemical speciation of arsenic and oxidative stress biomarkers were quantified in S. spallanzanii after a 30days transplant experiment into naturally acidified conditions in a Mediterranean vent system. No significant bioaccumulation of metals was observed in the thoracic tissues and branchial crowns after the translocation period, whereas variations occurred in the relative abundance of different arsenic compounds with the appearance of inorganic forms. The antioxidant system of translocated polychaetes exhibited a significant decrease of enzymatic activities of both catalase and glutathione peroxidases, and the impairment of the overall capability to neutralize hydroxyl radicals (OH). This highlighted an oxidative challenge primarily on the detoxification pathway of hydrogen peroxide. Overall low pH-elevated pCO2 may have detrimental effects on arsenic metabolism and oxidative status of S. spallanzanii, supporting the hypothesis of species-specific differences in vulnerability to ocean acidification.
authors.affiliation: Stazione Zoologica Anton Dohrn di Napoli, Villa Dohrn Punta S. Pietro, 80077 Ischia, NA, Italy. Electronic address: elenaricevuto@gmail.com.
authors.forename: E
authors.initials: E
authors.lastname: Ricevuto
authors.affiliation: Dipartimento di Scienze della Vita e dell'Ambiente, Università Politecnica delle Marche, Ancona, Italy.
authors.forename: I
authors.initials: I
authors.lastname: Lanzoni
authors.affiliation: Dipartimento di Scienze della Vita e dell'Ambiente, Università Politecnica delle Marche, Ancona, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Fattorini
authors.affiliation: Dipartimento di Scienze della Vita e dell'Ambiente, Università Politecnica delle Marche, Ancona, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Regoli
authors.affiliation: Stazione Zoologica Anton Dohrn di Napoli, Villa Dohrn Punta S. Pietro, 80077 Ischia, NA, Italy.
authors.forename: M C
authors.initials: MC
authors.lastname: Gambi
mesh_terms: D000818:Animals; D000845:Annelida / Q000378:metabolism*; D001151:Arsenic / Q000378:metabolism; D001152:Arsenicals / Q000378:metabolism*; D002101:Cacodylic Acid; D004784:Environmental Monitoring / Q000379:methods*; D060086:Hydrothermal Vents / Q000737:chemistry*; D018384:Oxidative Stress; D011077:Polychaeta / Q000378:metabolism
chemical_list: D001152:Arsenicals; D002101:Cacodylic Acid; D001151:Arsenic
keywords: Antioxidant activity; Ocean acidification; Oxidative stress; Polychaete; Trace metal