title: Preimplantation embryo-secreted factors modulate maternal gene expression in rat uterus.
abstract: In mammalian reproduction, embryo implantation into the uterus is spatiotemporally regulated by a complex process triggered by a number of factors. Although previous studies have suggested that uterine receptivity is mediated by blastocyst-derived factors, specific functions of embryos remain to be defined during preimplantation. Therefore, the present study was conducted to identify the maternal genes regulated by embryo-secreted factors in the rat uterus. RNA-sequencing (RNA-seq) data revealed that 10 genes are up-regulated in the delayed implantation uterus compared with the pseudopregnancy uterus. The RNA-seq results were further verified by real-time quantitative polymerase chain reaction. Sulf1 expression is significantly (P < 0.05) induced in the delayed implantation uterus, although Areg, Calca, Fxyd4 and Lamc3 show a definite but non-statistically significant increase in their expression levels. During early pregnancy, the levels of Areg, Calca, Fxyd4, Lamc3 and Sulf1 expression at 3.5 days post coitus (dpc) are significantly (P < 0.05) higher than those at 1.5 dpc. Treatment with embryo-conditioned media revealed that Lamc3 and Sulf1 are up-regulated compared with the other genes studied. Thus, embryo-derived factors regulate maternal gene expression, with Lamc3 and Sulf1 possibly being suitable markers for a response study of embryo-secreted factors to improve our understanding of embryo-maternal communication.
authors.affiliation: Department of Animal and Marine Bioresource Sciences, Graduate School Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka, 812-8581, Japan.
authors.forename: Kazuki
authors.initials: K
authors.lastname: Yamagami
authors.affiliation: Department of Animal and Marine Bioresource Sciences, Graduate School Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka, 812-8581, Japan.|Department of Genetics and Animal Breeding, Hajee Mohammad Danesh Science and Technology University, Dinajpur, 5200, Bangladesh.
authors.forename: M Rashedul
authors.initials: MR
authors.lastname: Islam
authors.affiliation: Department of Animal and Marine Bioresource Sciences, Graduate School Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka, 812-8581, Japan.
authors.forename: Yuka
authors.initials: Y
authors.lastname: Yoshii
authors.affiliation: Biotechnology Research Institute for Drug Discovery, National Institute of Advanced Industrial Science and Technology, 2-4-7 Aomi, Koto, Tokyo, 135-0064, Japan.
authors.forename: Kazuki
authors.initials: K
authors.lastname: Mori
authors.affiliation: Laboratory of Molecular Gene Technics, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka, 812-8581, Japan.
authors.forename: Kosuke
authors.initials: K
authors.lastname: Tashiro
authors.affiliation: Department of Animal and Marine Bioresource Sciences, Graduate School Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka, 812-8581, Japan. ny0531@agr.kyushu-u.ac.jp.|Laboratory of Reproductive Physiology and Biotechnology, Department of Animal and Marine Bioresource Sciences,, Graduate School Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka, 812-8581, Japan. ny0531@agr.kyushu-u.ac.jp.
authors.forename: Nobuhiko
authors.initials: N
authors.lastname: Yamauchi
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000378:metabolism*; D046149:Embryo Culture Techniques; D010064:Embryo Implantation / Q000235:genetics* / Q000502:physiology; D004622:Embryo, Mammalian / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D051381:Rats; D017208:Rats, Wistar; D014599:Uterus / Q000378:metabolism*
keywords: Embryo-secreted factors; Lamc3; RNA-sequencing; Sulf1; Uterus