title: The effects of different environmental pH on healing of tympanic membrane: an experimental study.
abstract: In this study, we investigated the effect of environmental pH on healing of acute rat tympanic membrane perforations. Twenty Wistar albino rats were divided into four groups and used in the study. A large myringotomy was performed in the posteroinferior quadrants of both tympanic membranes. In left ears, topical pH 4 standard calibration solution was used in groups 1 and 3, and pH 7 standard calibration solution was used in groups 2 and 4. Right ears served as controls, and allowed for spontaneous healing. The solutions were applied for 2 days in groups 1 and 2, and for 7 days in groups 3 and 4. Healing was assessed by macroscopic closure of the tympanic membrane perforation, and histopathological analysis of lamina propria edema, neovascularization, inflammatory cells, and fibroblastic reaction in the temporal bones. pH 7 and pH 4 groups were similar for macroscopic closure of perforation on day 2; however difference was significant on day 7. The fibroblastic activity was significantly less on days 2 and 7 in pH 4 group. On day 7, there were significant differences between pH 4 and pH 7, and pH 7 and control groups for inflammatory cell infiltration. In conclusion, clinical and histopathological results of this study indicated that acidic environmental pH speeded up and shortened wound-healing process. By building up optimum environmental pH, a healthy healing may be achieved in acute tympanic membrane perforations.
authors.affiliation: Otorhinolaryngology Clinic, Ministry of Health Ankara Education and Research Hospital, Mehmet Akif Ersoy Mah. 266.Cad. No:7C G3 Blok D:25 Yenimahalle, Ankara, Turkey.
authors.forename: Ahmet
authors.initials: A
authors.lastname: Akkoc
authors.affiliation: Otorhinolaryngology Clinic, Ministry of Health Ankara Education and Research Hospital, Mehmet Akif Ersoy Mah. 266.Cad. No:7C G3 Blok D:25 Yenimahalle, Ankara, Turkey.
authors.forename: Hatice
authors.initials: H
authors.lastname: Celik
authors.affiliation: Otorhinolaryngology Clinic, Ministry of Health Ankara Education and Research Hospital, Mehmet Akif Ersoy Mah. 266.Cad. No:7C G3 Blok D:25 Yenimahalle, Ankara, Turkey.
authors.forename: Necmi
authors.initials: N
authors.lastname: Arslan
authors.affiliation: Otorhinolaryngology Clinic, Ministry of Health Ankara Education and Research Hospital, Mehmet Akif Ersoy Mah. 266.Cad. No:7C G3 Blok D:25 Yenimahalle, Ankara, Turkey. suledemirci1@yahoo.com.tr.
authors.forename: Sule
authors.initials: S
authors.lastname: Demirci
authors.affiliation: Department of Pathology, Ministry of Health Ankara Education and Research Hospital, Ankara, Turkey.
authors.forename: Sema
authors.initials: S
authors.lastname: Hucumenoglu
authors.affiliation: Department of Pathology, Ministry of Health Ankara Education and Research Hospital, Ankara, Turkey.
authors.forename: Muzaffer
authors.initials: M
authors.lastname: Caydere
authors.affiliation: Department of Biostatistics, Ankara University Faculty of Medicine, Ankara, Turkey.
authors.forename: Derya
authors.initials: D
authors.lastname: Oztuna
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000737:chemistry; D001205:Ascorbic Acid / Q000008:administration & dosage / Q000737:chemistry; D002465:Cell Movement / Q000187:drug effects; D004847:Epithelial Cells / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism; D006851:Hydrochloric Acid / Q000008:administration & dosage / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008297:Male; D009092:Mucous Membrane / Q000473:pathology; D010710:Phosphates / Q000008:administration & dosage / Q000737:chemistry; D017680:Potassium Compounds / Q000008:administration & dosage / Q000737:chemistry; D017208:Rats, Wistar; D012972:Sodium Hydroxide / Q000008:administration & dosage / Q000737:chemistry; D018058:Tympanic Membrane Perforation / Q000188:drug therapy*; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000975:Antioxidants; D010710:Phosphates; D017680:Potassium Compounds; D012972:Sodium Hydroxide; D001205:Ascorbic Acid; D006851:Hydrochloric Acid; C018279:sodium phosphate
keywords: Epithelial migration; Fibroblastic reaction; Tympanic membrane perforation; Wound healing; pH