title: Pharmacological Actions of Hydrogen Sulfide Donors on Sympathetic Neurotransmission in the Bovine Anterior Uvea, In Vitro.
abstract: In the present study, we investigated the effect of three different sources of hydrogen sulfide (H2S) on sympathetic neurotransmission from isolated superfused bovine iris-ciliary bodies. The three agents under consideration were: ACS67, a hybrid of latanoprost and a H2S-donating moiety; L-cysteine, a substrate for endogenous production of H2S and GYY 4137, a slow donor of H2S. We also examined the contribution of prostaglandins to the pharmacological actions of the H2S donors on release of [(3)H]-norepinephrine ([(3)H]NE) triggered by electrical field stimulation. ACS67, L-cysteine and GYY 4137 caused a concentration-dependent inhibition of electrically-evoked [(3)H]NE release from isolated bovine iris-ciliary bodies without affecting basal [(3)H]NE efflux. The cyclooxygenase inhibitor, flurbiprofen enhanced the inhibitory action of ACS67 and L-cysteine on stimulated [(3)H]NE release. Both aminooxyacetic acid, an inhibitor of cystathionine-β-synthase and glibenclamide, a KATP channel blocker reversed the inhibition of evoked NE release induced by the H2S donors. We conclude that H2S donors can inhibit sympathetic neurotransmission from isolated bovine iris-ciliary bodies, an effect partially dependent on the in situ production of H2S and prostanoids, and is mediated by an action on KATP channels.
authors.affiliation: Department of Pharmacy Sciences, School of Pharmacy and Health Professions, Creighton University, Omaha, NE, 68178, USA.
authors.forename: Ankita
authors.initials: A
authors.lastname: Salvi
authors.affiliation: Department of Pharmacy Sciences, School of Pharmacy and Health Professions, Creighton University, Omaha, NE, 68178, USA.
authors.forename: Pratik
authors.initials: P
authors.lastname: Bankhele
authors.affiliation: Department of Pharmacy Sciences, School of Pharmacy and Health Professions, Creighton University, Omaha, NE, 68178, USA.
authors.forename: Jamal M
authors.initials: JM
authors.lastname: Jamil
authors.affiliation: Department of Pharmaceutical & Environmental Health Sciences, College of Pharmacy and Health Sciences, Texas Southern University, Houston, TX, 77004, USA.
authors.forename: Madhura
authors.initials: M
authors.lastname: Kulkarni-Chitnis
authors.affiliation: Department of Pharmaceutical & Environmental Health Sciences, College of Pharmacy and Health Sciences, Texas Southern University, Houston, TX, 77004, USA.
authors.forename: Ya Fatou
authors.initials: YF
authors.lastname: Njie-Mbye
authors.affiliation: Department of Pharmaceutical & Environmental Health Sciences, College of Pharmacy and Health Sciences, Texas Southern University, Houston, TX, 77004, USA.
authors.forename: Sunny E
authors.initials: SE
authors.lastname: Ohia
authors.affiliation: Department of Pharmacy Sciences, School of Pharmacy and Health Professions, Creighton University, Omaha, NE, 68178, USA. copere@creighton.edu.
authors.forename: Catherine A
authors.initials: CA
authors.lastname: Opere
mesh_terms: D000818:Animals; D002417:Cattle; D002924:Ciliary Body / Q000187:drug effects / Q000378:metabolism; D003545:Cysteine / Q000494:pharmacology; D004558:Electric Stimulation; D006862:Hydrogen Sulfide / Q000378:metabolism*; D066298:In Vitro Techniques; D009025:Morpholines / Q000494:pharmacology; D009638:Norepinephrine / Q000378:metabolism; D009946:Organothiophosphorus Compounds / Q000494:pharmacology; D011453:Prostaglandins / Q000378:metabolism; D011461:Prostaglandins F, Synthetic / Q000494:pharmacology; D013564:Sympathetic Nervous System / Q000187:drug effects* / Q000502:physiology; D009435:Synaptic Transmission; D008063:Thioctic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D014602:Uvea / Q000187:drug effects* / Q000502:physiology
chemical_list: C546828:ACS67 compound; C529376:GYY 4137; D009025:Morpholines; D009946:Organothiophosphorus Compounds; D011453:Prostaglandins; D011461:Prostaglandins F, Synthetic; D008063:Thioctic Acid; D003545:Cysteine; D009638:Norepinephrine; D006862:Hydrogen Sulfide
keywords: Anterior uvea; Hydrogen sulfide; Neurotransmission; [3H]-norepinephrine release