title: Cloning and sequence analysis of the LOC339524 gene in Sprague-Dawley rats.
abstract: We cloned the LOC339524 gene in Sprague-Dawley (SD) rats and analyzed the structure and function of the protein encoded by it. Based on the known human LOC339524 gene sequences, the full-length coding sequence of the LOC339524 gene in SD rats was cloned and amplified by the polymerase chain reaction using the complementary DNA of SD rats as a template. Bioinformatics analysis showed that the length of the cloned LOC339524 gene (GenBank accession No. KM224520) was 831 bp and it encoded a deduced protein of 276 amino acids. Sequence analysis revealed that the coded protein was identical to that produced in humans and its functional domain was located in the 138-236 amino acid fragments, a proline-rich region. Our results suggest that the encoded protein may be a significant regulator of the inflammatory response and may provide sufficient information to justify an in-depth investigation of the role of the LOC339524 gene.
authors.affiliation: Department of Anesthesiology, Xinqiao Hospital, Third Military Medical University, Chongqing, China.
authors.forename: Z H
authors.initials: ZH
authors.lastname: Long
authors.affiliation: Department of Anesthesiology, Xinqiao Hospital, Third Military Medical University, Chongqing, China.
authors.forename: H
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Anesthesiology, Xinqiao Hospital, Third Military Medical University, Chongqing, China.
authors.forename: F
authors.initials: F
authors.lastname: Chen
authors.affiliation: College of Basic Medical Sciences, Third Military Medical University, Chongqing, China.
authors.forename: L Y
authors.initials: LY
authors.lastname: Zou
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004474:Ecthyma, Contagious; D056426:Genetic Loci; D058977:Molecular Sequence Annotation; D008969:Molecular Sequence Data; D051381:Rats; D017207:Rats, Sprague-Dawley