title: Serum cytokine modulation after Staphylococcus hyicus infection in BALB/c mice.
abstract: Staphylococcal infection is a severe bacterial infection. Finding satisfactory predictable biological markers is essential for the treatment of this condition. In this study, we applied a 32-marker sandwich ELISA-based antibody array to evaluate cytokine changes in Staphylococcus hyicus-inoculated BALB/c mice at 24 and 48 h post infection. Among the cytokines detected, the expression levels of granulocyte colony stimulating factor (G-CSF), interleukin 6 (IL-6), macrophage inflammatory protein 2, and keratinocyte chemoattractant (KC) were increased to levels more than twice higher than those in the control group 24 h after infection, while the expression of interleukin 12p40p70 (IL-12p40p70) was decreased to less than half the level measured in the controls. The expression of G-CSF, IL-6, monocyte chemoattractant protei-5, chemokine ligand 11, and KC was upregulated 48 h post infection, whereas IL-12p40p70 expression was still significantly lower (P < 0.05). Among the detected cytokines, the expression levels of G-CSF, IL-6, and KC were constantly upregulated, while IL-12p40p70 was downregulated. This result was then validated by an ELISA assay analysis, which confirmed that G-CSF, IL-6, KC, and IL- 12p40p70 expression levels were specifically modulated after an S. hyicus bacterial infection, while granulocyte monocyte colony stimulation factor, IL-12, and IFNγ levels were significantly increased after a viral infection. Our study indicated the potential of cytokines G-CSF, IL-6, KC, and IL- 12p40p70 as markers for detecting S. hyicus infection. The results of this study may provide useful data for the appropriate use of medication following S. hyicus infection.
authors.affiliation: Institute of Animal Health, Guangdong Academy of Agriculture Sciences, Guangzhou, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Li
authors.affiliation: Institute of Animal Health, Guangdong Academy of Agriculture Sciences, Guangzhou, China.|Guangdong Open Laboratory of Veterinary Public Health, Guangdong Provincial Key Laboratory of Livestock Disease Prevention, Institute of Animal Health, Guandong Academy of Agricultural Sciences, Guangzhou, China.
authors.forename: L
authors.initials: L
authors.lastname: Wang
authors.affiliation: Institute of Animal Health, Guangdong Academy of Agriculture Sciences, Guangzhou, China.
authors.forename: L Y
authors.initials: LY
authors.lastname: Zhang
authors.affiliation: Institute of Animal Health, Guangdong Academy of Agriculture Sciences, Guangzhou, China.
authors.forename: Y L
authors.initials: YL
authors.lastname: Liu
authors.affiliation: Institute of Animal Health, Guangdong Academy of Agriculture Sciences, Guangzhou, China.
authors.forename: Z Y
authors.initials: ZY
authors.lastname: Jiang
authors.affiliation: Institute of Animal Health, Guangdong Academy of Agriculture Sciences, Guangzhou, China.
authors.forename: R J
authors.initials: RJ
authors.lastname: Cai
authors.affiliation: Guangdong Open Laboratory of Veterinary Public Health, Guangdong Provincial Key Laboratory of Livestock Disease Prevention, Institute of Animal Health, Guandong Academy of Agricultural Sciences, Guangzhou, China.
authors.forename: C X
authors.initials: CX
authors.lastname: Song
mesh_terms: D000818:Animals; D016207:Cytokines / Q000097:blood*; D051379:Mice; D008807:Mice, Inbred BALB C; D013203:Staphylococcal Infections / Q000097:blood*; D057788:Staphylococcus hyicus; D015854:Up-Regulation
chemical_list: D016207:Cytokines