title: Microbiome analyses of pacific white shrimp (Litopenaeus vannamei) collected from disparate geographical locations.
abstract: In this study, the tail muscle microbiota of pacific white shrimp (Litopenaeus vannamei) sourced from five countries across Central and South America and Southeast Asia were determined and compared. The genomic DNA was sequenced at around 10 × coverage for each geographical location and was assembled de novo for comparative analysis. The assembled sequences for all the lines were classified based on their similarity to the sequences in the public database. We found that there is high correlation among the microbiota of shrimp from disparate regions, as well as the presence of some DNA from bacteria known to cause food poisoning in humans. Sequencing data has been deposited at NCBI-SRA database and can be found under the BioProject ID PRJNA282154.
authors.affiliation: Genome Informatics Facility, Office of Biotechnology, Iowa State University, Ames, IA 50011, USA.
authors.forename: Arun S
authors.initials: AS
authors.lastname: Seetharam
authors.affiliation: Department of Animal Sciences, Iowa State University, Ames, IA 50011, USA.
authors.forename: Emily
authors.initials: E
authors.lastname: Kawaler
authors.affiliation: College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Du
authors.affiliation: Department of Animal Sciences, Iowa State University, Ames, IA 50011, USA.
authors.forename: Max F
authors.initials: MF
authors.lastname: Rothschild
authors.affiliation: Genome Informatics Facility, Office of Biotechnology, Iowa State University, Ames, IA 50011, USA.
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Severin
keywords: Geographical diversity; Litopenaeus vannamei; Microbiome; Next-generation sequencing; Shrimp