title: Effect of prenatal steroidal inhibition of sPLA2 in a rat model of preterm lung.
abstract: BACKGROUND
  Secretory phospholipase A2 regulates surfactant catabolism and inflammatory cascade. This enzyme is correlated with compliance, oxygenation and major outcomes in various forms of acute respiratory failure. Steroids inhibit secretory phospholipase A2 in cell culture and are widely used to boost surfactant production before preterm delivery. No data are available about the effect of antenatal steroids on secretory phospholipase A2 in the offspring: we aimed to study this effect in a rat model of preterm lung.
  
  
  METHODS
  Fifteen pregnant Wistar rats were randomized to receive betamethasone, dexamethasone or placebo at 20 and 21 days gestation. Newborn rats were supported for 8 h and then sacrificed: lung tissue was analysed for secretory phospholipase A2 expression and activity, inflammatory mediators and protein content. Lipidomics was analysed using liquid chromatography-mass spectrometry.
  
  
  RESULTS
  Secretory phospholipase A2 expression was significantly reduced by antenatal steroids (p < 0.001). Secretory phospholipase A2 activity, TNFα and lysophosphatidylethanolamine, a product of phospholipase reaction, were lowest in betamethasone-treated rats (p < 0.001). There was a strong correlation between secretory phospholipase A2 activity and lysophosphatidylethanolamine (r = 0.75; p = 0.001) and this remained significant after adjustment for total proteins or phospholipids.
  
  
  CONCLUSIONS
  Antenatal steroids decrease secretory phospholipase A2 in rat model of preterm lung.
authors.affiliation: NICU and Animal Lab, Dept of Paediatrics, University Hospital of Salamanca, Paseo de San Vicente182, 37007 Salamanca, Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Remesal
authors.affiliation: Division of Paediatrics and Neonatal Critical Care, FAME Dept, South Paris University Hospitals, Medical Center "A.Béclère"-APHP, 157 rue de la Porte de Trivaux, 92140 Clamart, Paris, France; Lab of Clinical Molecular Biology, Dept of Laboratory Medicine, University Hospital "A.Gemelli", Catholic University of the Sacred Heart, L.go A. Gemelli 8, 00168 Roma, Italy. Electronic address: daniele.deluca@aphp.fr.
authors.forename: Daniele
authors.initials: D
authors.lastname: De Luca
authors.affiliation: NICU and Animal Lab, Dept of Paediatrics, University Hospital of Salamanca, Paseo de San Vicente182, 37007 Salamanca, Spain.
authors.forename: Laura
authors.initials: L
authors.lastname: San Feliciano
authors.affiliation: Molecular Genetics and Pharmacogenetics Unit, Dept of Clinical Biochemistry, University Hospital of Salamanca, Pase de San Vicente182, 37007 Salamanca, Spain.
authors.forename: Maria
authors.initials: M
authors.lastname: Isidoro-Garcia
authors.affiliation: Lab of Clinical Molecular Biology, Dept of Laboratory Medicine, University Hospital "A.Gemelli", Catholic University of the Sacred Heart, L.go A. Gemelli 8, 00168 Roma, Italy.
authors.forename: Angelo
authors.initials: A
authors.lastname: Minucci
authors.affiliation: Lab of Clinical Molecular Biology, Dept of Laboratory Medicine, University Hospital "A.Gemelli", Catholic University of the Sacred Heart, L.go A. Gemelli 8, 00168 Roma, Italy.
authors.forename: Krizia
authors.initials: K
authors.lastname: Pocino
authors.affiliation: Research Unit on BioActive Molecules, Dept. of Biomedicinal Chemistry, Institute for Advanced Chemistry, Jordi Girona 18, 08034 Barcelona, Spain.
authors.forename: Josefina
authors.initials: J
authors.lastname: Casas
authors.affiliation: Research Unit on BioActive Molecules, Dept. of Biomedicinal Chemistry, Institute for Advanced Chemistry, Jordi Girona 18, 08034 Barcelona, Spain.
authors.forename: Gemma
authors.initials: G
authors.lastname: Fabrias
authors.affiliation: Lab of Clinical Molecular Biology, Dept of Laboratory Medicine, University Hospital "A.Gemelli", Catholic University of the Sacred Heart, L.go A. Gemelli 8, 00168 Roma, Italy.
authors.forename: Ettore D
authors.initials: ED
authors.lastname: Capoluongo
authors.affiliation: NICU and Animal Lab, Dept of Paediatrics, University Hospital of Salamanca, Paseo de San Vicente182, 37007 Salamanca, Spain.
authors.forename: Dolores Ludena
authors.initials: DL
authors.lastname: de la Cruz
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D001623:Betamethasone / Q000494:pharmacology; D003907:Dexamethasone / Q000494:pharmacology; D005260:Female; D018836:Inflammation Mediators / Q000378:metabolism; D050356:Lipid Metabolism / Q000187:drug effects; D008168:Lung / Q000201:enzymology* / Q000473:pathology; D008246:Lysophospholipids / Q000378:metabolism; D054497:Phospholipases A2, Secretory / Q000037:antagonists & inhibitors*; D011247:Pregnancy; D047928:Premature Birth / Q000473:pathology*; D011663:Pulmonary Surfactants / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013256:Steroids / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D018836:Inflammation Mediators; D008246:Lysophospholipids; D011663:Pulmonary Surfactants; D013256:Steroids; D014409:Tumor Necrosis Factor-alpha; C008301:lysophosphatidylethanolamine; D003907:Dexamethasone; D001623:Betamethasone; D054497:Phospholipases A2, Secretory
keywords: Preterm neonate; Rat; Secretory phospholipase A2; Steroids; Surfactant