title: Plasma cholinesterase activity as a biomarker for quantifying exposure of green sturgeon to carbaryl following applications to control burrowing shrimp in Washington State.
abstract: Willapa Bay (Washington State, USA) has been 1 of the rare intertidal locations where large-scale pesticide applications occur. Until recently, carbaryl was applied to control burrowing shrimp that decrease commercial oyster productivity. The bay is a critical habitat for green sturgeon (Acipenser medirostris), an anadromous species listed as threatened under the US Endangered Species Act. However, the hazard that carbaryl poses is unknown. Surrogate seawater-acclimated white sturgeon (A. transmontanus) were exposed to 0 μg L(-1) , 30 μg L(-1) , 100 μg L(-1) , 300 μg L(-1) , 1000 μg L(-1) , and 3000 μg L(-1) carbaryl for 6 h, and brain acetylcholinesterase (AChE) and plasma butyrylcholinesterase (BChE) activities were measured. Enzyme recovery was measured in an additional cohort exposed to 1000 μg L(-1) carbaryl for 6 h. Activity of AChE was reduced (p ≤ 0.001) at concentrations ≥ 100 μg L(-1) with recovery in the 1000 μg L(-1) cohort by 72 h. Surprisingly, BChE activity was greater than controls at concentrations ≥ 300 μg L(-1) (p > 0.05), a finding confirmed in additional fish exposed to 3000 μg L(-1) for 6 h (+30%, p < 0.001) with apparent recovery by 48 h. Plasma samples were collected from free-living green sturgeon before and 4 d to 5 d after application of carbaryl in Willapa Bay. Activity of BChE after application was reduced 28% (p < 0.001), indicating exposure to the pesticide. However, the lack of congruence between BChE and AChE activity in captive white sturgeon exposed to carbaryl indicates that further studies are needed to better understand the risk carbaryl exposure poses to green sturgeon. Environ Toxicol Chem 2016;35:2003-2015. © 2015 SETAC.
authors.affiliation: Washington Cooperative Fish and Wildlife Research Unit, School of Aquatic and Fishery Sciences, University of Washington, Seattle, Washington, USA.
authors.forename: Alexandra T
authors.initials: AT
authors.lastname: Troiano
authors.affiliation: Washington Cooperative Fish and Wildlife Research Unit, School of Aquatic and Fishery Sciences, University of Washington, US Geological Survey, Seattle, Washington, USA.
authors.forename: Christian E
authors.initials: CE
authors.lastname: Grue
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D015415:Biomarkers / Q000097:blood; D001921:Brain / Q000187:drug effects / Q000201:enzymology; D002091:Butyrylcholinesterase / Q000097:blood*; D012721:Carbaryl / Q000633:toxicity*; D003445:Crustacea / Q000187:drug effects* / Q000254:growth & development; D004305:Dose-Response Relationship, Drug; D017753:Ecosystem; D004781:Environmental Exposure / Q000032:analysis*; D005399:Fishes / Q000097:blood*; D010125:Ostreidae / Q000254:growth & development; D012623:Seawater / Q000737:chemistry*; D014861:Washington; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D015415:Biomarkers; D014874:Water Pollutants, Chemical; D000110:Acetylcholinesterase; D002091:Butyrylcholinesterase; D012721:Carbaryl
keywords: Acetylcholinesterase inhibitors; Butyrylcholinesterase; Carbaryl; Estuarine toxicology; Hazard/risk assessment; Sturgeon