title: 3D Image-Guided Automatic Pipette Positioning for Single Cell Experiments in vivo.
abstract: We report a method to facilitate single cell, image-guided experiments including in vivo electrophysiology and electroporation. Our method combines 3D image data acquisition, visualization and on-line image analysis with precise control of physical probes such as electrophysiology microelectrodes in brain tissue in vivo. Adaptive pipette positioning provides a platform for future advances in automated, single cell in vivo experiments.
authors.affiliation: Allen Institute for Brain Science, Seattle, WA, USA.
authors.forename: Brian
authors.initials: B
authors.lastname: Long
authors.affiliation: Allen Institute for Brain Science, Seattle, WA, USA.
authors.forename: Lu
authors.initials: L
authors.lastname: Li
authors.affiliation: Allen Institute for Brain Science, Seattle, WA, USA.
authors.forename: Ulf
authors.initials: U
authors.lastname: Knoblich
authors.affiliation: Allen Institute for Brain Science, Seattle, WA, USA.
authors.forename: Hongkui
authors.initials: H
authors.lastname: Zeng
authors.affiliation: Allen Institute for Brain Science, Seattle, WA, USA.
authors.forename: Hanchuan
authors.initials: H
authors.lastname: Peng
mesh_terms: D000818:Animals; D001331:Automation; D021621:Imaging, Three-Dimensional; D008822:Mice, Transgenic; D009474:Neurons / Q000166:cytology; D059010:Single-Cell Analysis / Q000379:methods*; D013194:Staining and Labeling