title: Evaluation of physicochemical and biological properties of chitosan/poly (vinyl alcohol) polymer blend membranes and their correlation for Vero cell growth.
abstract: The blend membranes with varying weight ratios of chitosan/poly (vinyl alcohol) (CS/PVA) (1:0, 1:1, 1:2.5, 1.5:1, 1.5: 2.5) were prepared using solvent casting method and were evaluated for their potential application in single-use membrane bioreactors (MBRs). The physicochemical properties of the prepared membranes were investigated for chemical interactions (FTIR), surface morphology (SEM), water uptake, protein sorption (qe), ammonia sorption and growth kinetics of Vero cells. CS/PVA blend membrane having weight ratio of 1.5:1 had shown enhanced membrane flexibility, reduced water uptake, less protein sorption and no ammonium sorption compared to CS membrane. This blend membrane also showed comparatively enhanced higher specific growth rate (0.82/day) of Vero cells. Improved physicochemical properties and growth kinetics obtrude CS/PVA (1.5:1) as a potential surface for adhesion and proliferation with possible application in single use membrane bioreactors. Additionally, new insight explaining correlation between water holding (%) of CS/PVA (1.5:1) blend membrane and doubling time (td) of Vero cells is proposed.
authors.affiliation: Department of Biotechnology, Jaypee Institute of Information Technology, A-10, Sector-62, Noida 201307, UP, India.
authors.forename: Parul
authors.initials: P
authors.lastname: Sharma
authors.affiliation: Department of Biotechnology, Jaypee Institute of Information Technology, A-10, Sector-62, Noida 201307, UP, India.
authors.forename: Garima
authors.initials: G
authors.lastname: Mathur
authors.affiliation: Physics and Engineering of Carbon, Division of Materials Physics and Engineering, CSIR-National Physical Laboratory, Dr K. S. Krishnan Marg, New Delhi 110012, India.
authors.forename: Sanjay R
authors.initials: SR
authors.lastname: Dhakate
authors.affiliation: Department of Biochemical Engineering and Biotechnology, IIT Delhi, Hauz Khas, New Delhi 110016, India.
authors.forename: Subhash
authors.initials: S
authors.lastname: Chand
authors.affiliation: Department of Physics and Materials Science and Engineering, Jaypee Institute of Information Technology, A-10, Sector-62, Noida 201307, UP, India.
authors.forename: Navendu
authors.initials: N
authors.lastname: Goswami
authors.affiliation: Department of Biotechnology, Jaypee Institute of Information Technology, A-10, Sector-62, Noida 201307, UP, India.
authors.forename: Sanjeev K
authors.initials: SK
authors.lastname: Sharma
authors.affiliation: Department of Biotechnology, Jaypee Institute of Information Technology, A-10, Sector-62, Noida 201307, UP, India. Electronic address: ashiitd@yahoo.com.
authors.forename: Ashwani
authors.initials: A
authors.lastname: Mathur
mesh_terms: D000327:Adsorption; D000818:Animals; D048708:Cell Growth Processes; D049109:Cell Proliferation; D055598:Chemical Phenomena; D048271:Chitosan / Q000737:chemistry*; D002522:Chlorocebus aethiops; D007700:Kinetics; D008566:Membranes / Q000254:growth & development*; D008855:Microscopy, Electron, Scanning; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D014709:Vero Cells / Q000166:cytology; D014867:Water / Q000737:chemistry
chemical_list: D014867:Water; D011142:Polyvinyl Alcohol; D048271:Chitosan
keywords: Chitosan; Growth kinetics; Membrane; Sorption