title: Stimulation of laccases from Trametes pubescens: Use in dye decolorization and cotton bleaching.
abstract: The production of laccases from Trametes pubescens was investigated along with the role of nutrients and elicitors. Copper proved to be a fundamental inducer, although productivity yields were consistently enhanced only in the presence of additional compounds (textile dyes). Using a central composite design, the optimal culture condition was examined, by taking into consideration the three distinct variables and their combinatorial effect. The 290 U ml(-1) of laccases were produced after setting nitrogen, copper, and reactive blue 19 concentration; in a bioreactor, activity recovery was lower (90 U ml(-1)) and pellet morphology was different. The activity of the laccase crude extract was maximal at 60°C and stable for 14 h at 50°C and for 2 months at pH 6 and room temperature. The biotechnological potential was assessed, confirming the capacity to decolorize single or mixed solutions of textile dyes and to enhance the whitening yield of raw cotton fibers, working in synergism with the conventional H2O2-based method.
authors.affiliation: a Department of Life Sciences and Systems Biology , University of Torino , Torino , Italy.
authors.forename: Federica
authors.initials: F
authors.lastname: Spina
authors.affiliation: b Earth and Life Institute , Université Catholique de Louvain , Louvain-la-Neuve , Belgium.
authors.forename: Charles
authors.initials: C
authors.lastname: Junghanns
authors.affiliation: c Divisione Stazione Sperimentale per la Seta , Innovhub-SSI , Milano , Italy.
authors.forename: Ilaria
authors.initials: I
authors.lastname: Donelli
authors.affiliation: b Earth and Life Institute , Université Catholique de Louvain , Louvain-la-Neuve , Belgium.
authors.forename: Rakesh
authors.initials: R
authors.lastname: Nair
authors.affiliation: b Earth and Life Institute , Université Catholique de Louvain , Louvain-la-Neuve , Belgium.
authors.forename: Philippe
authors.initials: P
authors.lastname: Demarche
authors.affiliation: a Department of Life Sciences and Systems Biology , University of Torino , Torino , Italy.
authors.forename: Alice
authors.initials: A
authors.lastname: Romagnolo
authors.affiliation: c Divisione Stazione Sperimentale per la Seta , Innovhub-SSI , Milano , Italy.
authors.forename: Giuliano
authors.initials: G
authors.lastname: Freddi
authors.affiliation: b Earth and Life Institute , Université Catholique de Louvain , Louvain-la-Neuve , Belgium.
authors.forename: Spiros N
authors.initials: SN
authors.lastname: Agathos
authors.affiliation: a Department of Life Sciences and Systems Biology , University of Torino , Torino , Italy.
authors.forename: Giovanna Cristina
authors.initials: GC
authors.lastname: Varese
mesh_terms: D019149:Bioreactors; D003116:Color; D004396:Coloring Agents / Q000737:chemistry*; D032561:Cotton Fiber; D003470:Culture Media; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D042845:Laccase / Q000096:biosynthesis / Q000378:metabolism*; D013696:Temperature; D055454:Trametes / Q000201:enzymology*
chemical_list: D004396:Coloring Agents; D003470:Culture Media; D042845:Laccase
keywords: Biobleaching; central composite design; fungi; laccases; textile