title: Methods for the cultivation of ciliated protozoa from the large intestine of horses.
abstract: This paper describes cultivation methods for ciliates from the digestive tract of horses. Members of three different genera were successfully grown in vitro for short periods of time. However, only cells belonging to the genus Blepharocorys, which resides in the horse's large intestine, were maintained for longer periods. This Blepharocorys culture was successfully grown in vitro after inoculation of freshly excreted horse faeces in culture medium containing a population of bacteria. The ciliates survived for over six months, and the density of their population varied between 1.7 × 10(3) and 2.4 × 10(3) cells mL(-1). Favourable conditions for the prolonged cultivation of this ciliate were observed when the medium was prepared by mixing horse faeces and 'caudatum' salt solution in a 1:1 V/V ratio together with food (60% powdered meadow hay, 16% wheat gluten, 12% barley flour and 12% microcrystalline cellulose) supplied as 0.20 mg mL(-1) culture per day.
authors.affiliation: The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jabłonna, Poland g.belzecki@ifzz.pl.
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Bełżecki
authors.affiliation: The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jabłonna, Poland.
authors.forename: Renata
authors.initials: R
authors.lastname: Miltko
authors.affiliation: The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jabłonna, Poland.
authors.forename: Tadeusz
authors.initials: T
authors.lastname: Michałowski
authors.affiliation: Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Aberystwyth, SY23 3DA, UK.
authors.forename: Neil R
authors.initials: NR
authors.lastname: McEwan
mesh_terms: D000818:Animals; D016798:Ciliophora / Q000254:growth & development* / Q000378:metabolism; D016770:Ciliophora Infections / Q000469:parasitology / Q000662:veterinary*; D003470:Culture Media / Q000737:chemistry / Q000378:metabolism; D005243:Feces / Q000469:parasitology; D006734:Horse Diseases / Q000469:parasitology*; D006736:Horses; D007420:Intestine, Large / Q000469:parasitology*; D010274:Parasitology / Q000379:methods*
chemical_list: D003470:Culture Media
keywords: Blepharocorys; cultivation; horse; in vitro; protozoa