title: Synergistic effect of radon and sodium arsenite on DNA damage in HBE cells.
abstract: Human epidemiological studies showed that radon and arsenic exposures are major risk factors for lung cancer in Yunnan tin miners. However, biological evidence for this phenomenon is absent. In this study, HBE cells were exposed to different concentrations of sodium arsenite, different radon exposure times, or a combination of these two factors. The results showed a synergistic effect of radon and sodium arsenite in cell cytotoxicity as determined by cell viability. Elevated intracellular ROS levels and increased DNA damage indexed by comet assay and γ-H2AX were detected. Moreover, DNA HR repair in terms of Rad51 declined when the cells were exposed to both radon and sodium arsenite. The synergistic effect of radon and sodium arsenite in HBE cells may be attributed to the enhanced DSBs and inhibited HR pathway upon co-exposure.
authors.affiliation: Department of Toxicology, School of Public Health, Medical College of Soochow University, No.199 Ren-Ai Road, Suzhou 215123, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Toxicology, School of Public Health, Medical College of Soochow University, No.199 Ren-Ai Road, Suzhou 215123, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Sun
authors.affiliation: Department of Toxicology, School of Public Health, Medical College of Soochow University, No.199 Ren-Ai Road, Suzhou 215123, China.
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Toxicology, School of Public Health, Medical College of Soochow University, No.199 Ren-Ai Road, Suzhou 215123, China.
authors.forename: Jihua
authors.initials: J
authors.lastname: Nie
authors.affiliation: Department of Toxicology, School of Public Health, Medical College of Soochow University, No.199 Ren-Ai Road, Suzhou 215123, China.
authors.forename: Zhihai
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Department of Toxicology, School of Public Health, Medical College of Soochow University, No.199 Ren-Ai Road, Suzhou 215123, China. Electronic address: annine@suda.edu.cn.
authors.forename: Yan
authors.initials: Y
authors.lastname: An
authors.affiliation: Department of Toxicology, School of Public Health, Medical College of Soochow University, No.199 Ren-Ai Road, Suzhou 215123, China. Electronic address: tongjian@suda.edu.cn.
authors.forename: Jian
authors.initials: J
authors.lastname: Tong
mesh_terms: D018053:Arsenites / Q000633:toxicity*; D001980:Bronchi / Q000166:cytology*; D018797:Cell Cycle Proteins; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004249:DNA Damage; D004260:DNA Repair / Q000187:drug effects; D004268:DNA-Binding Proteins; D004357:Drug Synergism; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D009687:Nuclear Proteins / Q000378:metabolism; D010750:Phosphoproteins / Q000378:metabolism; D011886:Radon / Q000633:toxicity*; D017382:Reactive Oxygen Species / Q000378:metabolism; D017670:Sodium Compounds / Q000633:toxicity*
chemical_list: D018053:Arsenites; D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D010750:Phosphoproteins; C429022:RAD21 protein, human; D017382:Reactive Oxygen Species; D017670:Sodium Compounds; C017947:sodium arsenite; D011886:Radon
keywords: DNA damage; Radon; Sodium arsenite; Synergistic effect