title: Tissue MALDI Mass Spectrometry Imaging (MALDI MSI) of Peptides.
abstract: Matrix assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) is a technique to visualize molecular features of tissues based on mass detection. This chapter focuses on MALDI MSI of peptides and provides detailed operational instructions for sample preparation of cryoconserved and formalin-fixed paraffin-embedded (FFPE) tissue. Besides sample preparation we provide protocols for the MALDI measurement, tissue staining, and data analysis. On-tissue digestion and matrix application are described for two different commercially available and commonly used spraying devices: the SunCollect (SunChrom) and the ImagePrep (Bruker Daltonik GmbH).
authors.affiliation: Leibniz-Institut für Analytische Wissenschaften-ISAS-e.V., Dortmund, Germany.
authors.forename: Birte
authors.initials: B
authors.lastname: Beine
authors.affiliation: Clinical Proteomics, Medizinisches Proteom-Center, Ruhr-University, Bochum, Germany.
authors.forename: Hanna C
authors.initials: HC
authors.lastname: Diehl
authors.affiliation: Leibniz-Institut für Analytische Wissenschaften-ISAS-e.V., Dortmund, Germany.
authors.forename: Helmut E
authors.initials: HE
authors.lastname: Meyer
authors.affiliation: Leibniz-Institut für Analytische Wissenschaften-ISAS-e.V., Dortmund, Germany. corinna.henkel@isas.de.
authors.forename: Corinna
authors.initials: C
authors.lastname: Henkel
mesh_terms: D000818:Animals; D003584:Cytological Techniques; D006801:Humans; D010455:Peptides / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D010455:Peptides
keywords: Cryoconserved; Formalin-fixed paraffin-embedded (FFPE); ImagePrep; MALDI imaging; MSI; Matrix application; Peptide; SunCollect; Trypsin