title: Use of Cationized Ferritin Nanoparticles to Measure Renal Glomerular Microstructure with MRI.
abstract: Magnetic resonance imaging (MRI) is becoming important for whole-kidney assessment of glomerular morphology, both in vivo and ex vivo. MRI-based renal morphological measurements can be made in intact organs and allow direct measurements of every perfused glomerulus. Cationic ferritin (CF) is used as a superparamagnetic contrast agent for MRI. CF binds to the glomerular basement membrane after intravenous injection, allowing direct, whole-kidney measurements of glomerular number, volume, and volume distribution. Here we describe the production, testing, and use of CF as an MRI contrast agent for quantitative glomerular morphology in intact mouse, rat, and human kidneys.
authors.affiliation: Department of Biology, College of Natural Sciences, University of Hawaii at Manoa, 2540 Campus Rd., Honolulu, HI, 96822, USA. kevinben@hawaii.edu.
authors.forename: Kevin M
authors.initials: KM
authors.lastname: Bennett
authors.affiliation: Department of Radiology, Washington University School of Medicine, Saint Louis, MO, USA.
authors.forename: Scott C
authors.initials: SC
authors.lastname: Beeman
authors.affiliation: Department of Physics, College of Natural Sciences, University of Hawaii at Manoa, Honolulu, HI, USA.
authors.forename: Edwin J
authors.initials: EJ
authors.lastname: Baldelomar
authors.affiliation: School of Computing, Informatics, and Decision Systems Engineering, Arizona State University, Tempe, AZ, USA.
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.affiliation: School of Computing, Informatics, and Decision Systems Engineering, Arizona State University, Tempe, AZ, USA.
authors.forename: Teresa
authors.initials: T
authors.lastname: Wu
authors.affiliation: College of Tropical Agriculture and Human Resources, University of Hawaii at Manoa, Honolulu, HI, USA.
authors.forename: Bradley D
authors.initials: BD
authors.lastname: Hann
authors.affiliation: Department of Anatomy and Developmental Biology, Monash University, Melbourne, VIC, Australia.
authors.forename: John F
authors.initials: JF
authors.lastname: Bertram
authors.affiliation: Division of Nephrology, Department of Pediatrics, University of Virginia Medical Center, Charlottesville, VA, USA.
authors.forename: Jennifer R
authors.initials: JR
authors.lastname: Charlton
mesh_terms: D000818:Animals; D005293:Ferritins / Q000737:chemistry; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007678:Kidney Glomerulus / Q000166:cytology* / Q000648:ultrastructure*; D008279:Magnetic Resonance Imaging / Q000379:methods; D053768:Metal Nanoparticles; D051379:Mice; D051381:Rats
chemical_list: C010297:polycationic ferritin; D005293:Ferritins
keywords: Cationic ferritin; Chronic kidney disease; Contrast agent; Glomerulus; Kidney; Magnetic resonance imaging; Morphology