title: Protective Effects of Curcumin on Intestinal Damage in Cholestatic Rats.
abstract: BACKGROUND
  The aim of this study was to evaluate the possible protective effects of curcumin on oxidative stress, cell proliferation, and apoptosis in the rat intestinal mucosa after bile duct ligation (BDL).
  
  
  METHODS
  A total of 18 male Sprague Dawley rats were divided into three groups: sham control, BDL and BDL+curcumin; each group contain six animals. The rats in the curcumin-treated group were given curcumin (100 mg/kg) once a day orally for 14 days, starting 3 days prior to BDL operation. Following 14 days of treatment, all the animals were decapitated and intestinal tissues samples obtained for biochemical and histopathological investigation.
  
  
  RESULTS
  Curcumin treatment was found to significantly lower elevated tissue malondialdehyde levels and myeloperoxidase activity, and to raise reduced glutathione levels in intestinal tissues samples. BDL caused severe histopathological injury, including shortening of the villi, loss of villous epithelium, multiple erosions, inflammatory cell infiltration, necrosis, and hemorrhage into the intestinal wall. Curcumin treatment significantly attenuated the severity of intestinal injury, with inhibition of BDL-induced apoptosis and cell proliferation.
  
  
  CONCLUSIONS
  Curcumin treatment has a protective effect against intestinal damage induced by BDL. The ability of curcumin treatment is to inhibit BDL-induced oxidative stress, apoptosis, and cell proliferation.
authors.affiliation: a Department of Histology and Embriyology, Faculty of Medicine , Istanbul Medeniyet University , Istanbul , Turkey.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Kanter
authors.affiliation: b Clinics of Internal Medicine and Endocrinology , Istanbul Medeniyet University, Goztepe Education and Research Hospital , Istanbul , Turkey.
authors.forename: Mumtaz
authors.initials: M
authors.lastname: Takir
authors.affiliation: c Clinics of Family Medicine , Istanbul Medeniyet University, Goztepe Education and Research Hospital , Istanbul , Turkey.
authors.forename: Hasan Huseyin
authors.initials: HH
authors.lastname: Mutlu
authors.affiliation: d Faculty of Medicine , Medical University , Sofia , Bulgaria.
authors.forename: Betul
authors.initials: B
authors.lastname: Kanter
authors.affiliation: e Clinics of Internal Medicine , Istanbul Medeniyet University, Goztepe Education and Research Hospital , Istanbul , Turkey.
authors.forename: Osman
authors.initials: O
authors.lastname: Kostek
authors.affiliation: f Department of Biochemistry, Faculty of Medicine , Istanbul Medeniyet University , Istanbul , Turkey.
authors.forename: Aybala Erek
authors.initials: AE
authors.lastname: Toprak
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D001651:Cholestasis, Extrahepatic / Q000150:complications / Q000188:drug therapy*; D003135:Common Bile Duct / Q000601:surgery; D003474:Curcumin / Q000494:pharmacology*; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009336:Necrosis / Q000209:etiology; D018384:Oxidative Stress / Q000187:drug effects*; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000975:Antioxidants; D008315:Malondialdehyde; D009195:Peroxidase; D005978:Glutathione; D003474:Curcumin
keywords: PCNA; apoptosis; bile duct ligation; curcumin; intestinal damage; rat