title: Identification of functional genes involved in Cd(2+) response of Chinese surf clam (Mactra chinensis) through transcriptome sequencing.
abstract: The Chinese surf clam Mactra chinensis is an economically important bivalve species in the coastal waters of Liaoning and Shandong Province, China. In this study, we carried out transcriptome sequencing to develop molecular resources for M. chinensis and conducted an acute test of Cd(2+) stimulation through quantitative real-time PCR (qRT-PCR) to analyze the relative expression of six functional genes. A total of 100,839 transcripts and 56,712 unigenes were obtained from 39.9 million filtered reads and 21,305 unigenes were annotated by hitting against NCBI database. According to the results of qRT-PCR, heat shock protein 22 (Hsp22) and cytochrome P450 (CYP450(2C31)) were inhibited in the low concentration, and induced in the high concentration of Cd(2+); thioredoxin peroxidase (TPx-A) was at normal level in low concentration, but induced in high concentration of Cd(2+); glutathione peroxidase A (GPA), glutathione peroxidase 1 (GPA1) and Mn superoxide dismutase gene (MnSOD) were down-regulated when exposed to any treatment groups. Expression levels of the six functional genes following Cd(2+) exposure indicated that these genes were linked to environmental stress. Moreover, the present work enriched the molecule genetic data of M. chinensis.
authors.affiliation: National Marine Environmental Monitoring Center, Dalian 116023, China; Dalian Ocean University, Dalian 116023, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: National Marine Environmental Monitoring Center, Dalian 116023, China. Electronic address: hjli@nmemc.org.cn.
authors.forename: Hongjun
authors.initials: H
authors.lastname: Li
authors.affiliation: Dalian Ocean University, Dalian 116023, China.
authors.forename: Yanjie
authors.initials: Y
authors.lastname: Qin
authors.affiliation: Dalian Ocean University, Dalian 116023, China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Ye
authors.affiliation: Dalian Ocean University, Dalian 116023, China.
authors.forename: Min
authors.initials: M
authors.lastname: Liu
mesh_terms: D000818:Animals; D049872:Bivalvia / Q000187:drug effects / Q000235:genetics*; D002104:Cadmium / Q000633:toxicity*; D004781:Environmental Exposure; D020869:Gene Expression Profiling / Q000379:methods*; D005786:Gene Expression Regulation / Q000187:drug effects; D005979:Glutathione Peroxidase / Q000235:genetics; D000075322:Heavy Metal Poisoning; D011041:Poisoning / Q000235:genetics; D017423:Sequence Analysis, RNA / Q000379:methods*; D013482:Superoxide Dismutase / Q000235:genetics; D023382:Toxicity Tests, Acute
chemical_list: D002104:Cadmium; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase
keywords: Cd(2+) exposure; Functional gene; Illumina; Mactra chinensis; Quantitative real-time PCR; Transcriptome