title: Comparative analysis of Pasteurella multocida strains isolated from bovine respiratory infections.
abstract: Bovine respiratory disease (BRD) is the leading cause of significant economic losses in the intensive beef industry worldwide. Beside numerous risk factors Pasteurella multocida, which is regarded as a secondary pathogen, may play a role in the development of the disease. Previous studies of strains from swine pneumonia revealed that there are a few clones associated with clinical disease, suggesting that some strains may be more virulent than others. This linkage may be true in the BRD, however composition of P. multocida populations in the herds are slightly characterized. Thus, we decided to perform phenotypic and genotypic characterisation of strains isolated from calves with respiratory infection at 31 different herds in Hungary. The results demonstrated the presence of two dominant strain types. At the identical taxonomic background (P. multocida subsp. multocida) with slight phenotypic variability they could be separated by trehalose fermentation capacity, α-glucosidase activity and molecular fingerprint patterns of ERIC- and M13-PCR. Independent prevalence and geographical origin of the strain types may refer to their significance in the illness, but their comparison with strains isolated from healthy individuals is taken into consideration.
authors.affiliation: Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences , Budapest , Hungary.
authors.forename: Boglárka
authors.initials: B
authors.lastname: Sellyei
authors.affiliation: Veterinary Diagnostic Directorate, National Food Chain Safety Office , Budapest , Hungary.
authors.forename: Zsuzsanna
authors.initials: Z
authors.lastname: Rónai
authors.affiliation: Veterinary Diagnostic Directorate, National Food Chain Safety Office , Budapest , Hungary.
authors.forename: Szilárd
authors.initials: S
authors.lastname: Jánosi
authors.affiliation: Department of Microbiology and Infectious Diseases, Faculty of Veterinary Science, Szent István University , Budapest , Hungary.
authors.forename: László
authors.initials: L
authors.lastname: Makrai
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D005838:Genotype; D010326:Pasteurella Infections / Q000382:microbiology / Q000662:veterinary*; D016979:Pasteurella multocida / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D012141:Respiratory Tract Infections / Q000382:microbiology / Q000662:veterinary*
keywords: ERIC-PCR; M13-PCR; Pasteurella multocida; bovine respiratory disease; trehalose fermentation capacity; α-glucosidase activity