title: Ni(2+)-zeolite/ferrosphere and Ni(2+)-silica/ferrosphere beads for magnetic affinity separation of histidine-tagged proteins.
abstract: Magnetic Ni(2+)-zeolite/ferrosphere and Ni(2+)-silica/ferrosphere beads (Ni-ferrosphere beads - NFB) of a core-shell structure were synthesized starting from coal fly ash ferrospheres having diameters in the range of 0.063-0.050 mm. The strategy of NFB fabrication is an oriented chemical modification of the outer surface preserving the magnetic core of parent beads with the formation of micro-mesoporous coverings. Two routes of ferrosphere modification were realized, such as (i) hydrothermal treatment in an alkaline medium resulting in a NaP zeolite layer and (ii) synthesis of micro-mesoporous silica on the glass surface using conventional methods. Immobilization of Ni(2+) ions in the siliceous porous shell of the magnetic beads was carried out via (i) the ion exchange of Na(+) for Ni(2+) in the zeolite layer or (ii) deposition of NiO clusters in the zeolite and silica pores. The final NFB were tested for affinity in magnetic separation of the histidine-tagged green fluorescent protein (GFP) directly from a cell lysate. Results pointed to the high affinity of the magnetic beads towards the protein in the presence of 10 mM EDTA. The sorption capacity of the ferrosphere-based Ni-beads with respect to GFP was in the range 1.5-5.7 mg cm(-3).
authors.affiliation: Institute of Chemistry and Chemical Technology SB RAS, 50/24 Akademgorodok, Krasnoyarsk, 660036, Russia. tatiana_ver@mail.ru.
authors.forename: T A
authors.initials: TA
authors.lastname: Vereshchagina
authors.affiliation: Institute of Chemistry and Chemical Technology SB RAS, 50/24 Akademgorodok, Krasnoyarsk, 660036, Russia. tatiana_ver@mail.ru.
authors.forename: M A
authors.initials: MA
authors.lastname: Fedorchak
authors.affiliation: Institute of Chemistry and Chemical Technology SB RAS, 50/24 Akademgorodok, Krasnoyarsk, 660036, Russia. tatiana_ver@mail.ru.
authors.forename: O M
authors.initials: OM
authors.lastname: Sharonova
authors.affiliation: Institute of Chemistry and Chemical Technology SB RAS, 50/24 Akademgorodok, Krasnoyarsk, 660036, Russia. tatiana_ver@mail.ru.
authors.forename: E V
authors.initials: EV
authors.lastname: Fomenko
authors.affiliation: Institute of Chemistry and Chemical Technology SB RAS, 50/24 Akademgorodok, Krasnoyarsk, 660036, Russia. tatiana_ver@mail.ru.
authors.forename: N N
authors.initials: NN
authors.lastname: Shishkina
authors.affiliation: Institute of Chemistry and Chemical Technology SB RAS, 50/24 Akademgorodok, Krasnoyarsk, 660036, Russia. tatiana_ver@mail.ru.
authors.forename: A M
authors.initials: AM
authors.lastname: Zhizhaev
authors.affiliation: Institute of Biophysics SB RAS, 50/50 Akademgorodok, Krasnoyarsk, 660036, Russia. lfrank@yandex.ru.
authors.forename: A N
authors.initials: AN
authors.lastname: Kudryavtsev
authors.affiliation: Institute of Biophysics SB RAS, 50/50 Akademgorodok, Krasnoyarsk, 660036, Russia. lfrank@yandex.ru and Siberian Federal University, 79 Svobodnyi Avenue, Krasnoyarsk, 660041, Russia. anshits@icct.ru.
authors.forename: L A
authors.initials: LA
authors.lastname: Frank
authors.affiliation: Institute of Chemistry and Chemical Technology SB RAS, 50/24 Akademgorodok, Krasnoyarsk, 660036, Russia. tatiana_ver@mail.ru and Siberian Federal University, 79 Svobodnyi Avenue, Krasnoyarsk, 660041, Russia. anshits@icct.ru.
authors.forename: A G
authors.initials: AG
authors.lastname: Anshits
mesh_terms: D000818:Animals; D004926:Escherichia coli / Q000737:chemistry / Q000166:cytology; D049452:Green Fluorescent Proteins / Q000737:chemistry / Q000302:isolation & purification*; D006639:Histidine / Q000737:chemistry*; D058085:Iron Compounds / Q000737:chemistry*; D060328:Magnetic Phenomena; D009532:Nickel / Q000737:chemistry*; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D010316:Particle Size; D016062:Porosity; D037401:Scyphozoa; D012822:Silicon Dioxide / Q000737:chemistry*; D013499:Surface Properties; D017641:Zeolites / Q000737:chemistry*
chemical_list: D058085:Iron Compounds; D009942:Organometallic Compounds; D017641:Zeolites; D049452:Green Fluorescent Proteins; D006639:Histidine; D012822:Silicon Dioxide; D009532:Nickel