title: Monoclonal antibody-escape variant of dengue virus serotype 1: Genetic composition and envelope protein expression.
abstract: Monoclonal antibody-escape variant of dengue virus type 1 (MabEV DEN-1) was discovered and isolated in an outbreak of dengue in Klang Valley, Malaysia from December 2004 to March 2005. This study was done to investigate whether DEN152 (an isolate of MabEV DEN-1) is a product of recombination event or not. In addition, the non-synonymous mutations that correlate with the monoclonal antibody-escape variant were determined in this study. The genomes of DEN152 and two new DEN-1 isolates, DENB04 and DENK154 were completely sequenced, aligned, and compared. Phylogenetic tree was plotted and the recombination event on DEN152 was investigated. DEN152 is sub-grouped under genotype I and is closely related genetically to a DEN-1 isolated in Japan in 2004. DEN152 is not a recombinant product of any parental strains. Four amino acid substitutions were unique only to DEN 152. These amino acid substitutions were (Ser)[326](Leu), (Ser)[340](Leu) at the deduced E protein, (Ile)[250](Thr) at NS1 protein, and (Thr)[41](Ser) at NS5 protein. Thus, DEN152 is an isolate of the emerging monoclonal antibody-escape variant DEN-1 that escaped diagnostic laboratory detection.
authors.affiliation: National Public Health Laboratory, Lot 1853 Kg. Melayu, 47000, Sungai Buloh, Selangor, Malaysia.
authors.forename: Y K
authors.initials: YK
authors.lastname: Chem
authors.affiliation: Temasek Lifesciences Laboratory, 1 Research Link, National University of Singapore, 117604 Singapore.
authors.forename: K B
authors.initials: KB
authors.lastname: Chua
authors.affiliation: University of Tunku Abdul Rahman, Jalan Bandar Sg. Long, 43000 Sungai Long, Malaysia.
authors.forename: Y
authors.initials: Y
authors.lastname: Malik
authors.affiliation: International Medical University, 126 Jalan Jalil Perkasa 19, Bukit Jalil 57000, Kuala Lumpur, Malaysia.
authors.forename: K
authors.initials: K
authors.lastname: Voon
mesh_terms: D019943:Amino Acid Substitution; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000914:Antibodies, Viral / Q000276:immunology*; D003715:Dengue / Q000453:epidemiology / Q000821:virology*; D003716:Dengue Virus / Q000145:classification / Q000235:genetics / Q000276:immunology*; D004196:Disease Outbreaks; D016679:Genome, Viral; D006801:Humans; D008296:Malaysia / Q000453:epidemiology; D020125:Mutation, Missense; D010802:Phylogeny; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D065288:Serogroup; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D014759:Viral Envelope Proteins