title: Serotonin modulation in neonatal age does not impair cardiovascular physiology in adult female rats: Hemodynamics and oxidative stress analysis.
abstract: OBJECTIVE
  The present study investigates the effects of neonatal serotonin modulation in female rats on cardiac parameters related to hemodynamics and oxidative metabolism in the mature animal.
  
  
  METHODS
  Female Wistar rat pups were administered daily subcutaneous injections of fluoxetine (Fx-treated group) or vehicle solution (Ct-group) from the 1st to 21st day of life. At 60days of age, animals from both groups were either used for cardiovascular evaluation or sacrificed for tissue collection for biochemical assays.
  
  
  RESULTS
  We found that body weight in the Fx-treated group was less than that in the control. When analyzing hemodynamic parameters (i.e., arterial blood pressure, heart rate-HR, sympathetic and vagal tonus, or intrinsic HR), we did not observe significant difference in the Fx-treated group. Evaluating oxidative stress in brainstem and heart by measuring carbonyl content and malondialdehyde-MDA formation, we observe a decrease in carbonyl content only in the Fx-treated group (60.3%, in brainstem; 58.2%, in heart), without difference in the MDA levels. This observation is consonant with an increase in superoxide dismutase-SOD and catalase-CAT activity in brainstem and heart in the Fx-treated group (SOD: 82.7% and CAT: 23.7 in brainstem; SOD: 60.6%, and CAT: 40.7 in heart), with no changes in glutathione S-transferase activity and reduced glutathione levels. With regard to oxidative metabolism markers, citrate synthase activity was higher in brainstem in the Fx-treated group (20%).
  
  
  CONCLUSIONS
  Our data suggest that serotonin modulation by Fx-treatment at an early age does not induce hemodynamic alteration, although it modulates oxidative metabolism in cardiac-related tissues.
authors.affiliation: Laboratory of Biochemistry and Exercise Biochemistry, CAV-Federal University of Pernambuco, Vitoria de Santo Antão, PE, Brazil.
authors.forename: Glauber Ruda F
authors.initials: GR
authors.lastname: Braz
authors.affiliation: Laboratory of Biochemistry and Exercise Biochemistry, CAV-Federal University of Pernambuco, Vitoria de Santo Antão, PE, Brazil.
authors.forename: Anderson Apolonio
authors.initials: AA
authors.lastname: Pedroza
authors.affiliation: Department of Physical Education and Sports Science, CAV-Federal University of Pernambuco, Vitoria de Santo Antão, PE, Brazil.
authors.forename: Viviane O
authors.initials: VO
authors.lastname: Nogueira
authors.affiliation: Department of Physical Education and Sports Science, CAV-Federal University of Pernambuco, Vitoria de Santo Antão, PE, Brazil.
authors.forename: Monique Assis
authors.initials: MA
authors.lastname: de Vasconcelos Barros
authors.affiliation: Laboratory of Biochemistry and Exercise Biochemistry, CAV-Federal University of Pernambuco, Vitoria de Santo Antão, PE, Brazil.
authors.forename: Cristiane
authors.initials: C
authors.lastname: de Moura Freitas
authors.affiliation: Department of Physical Education and Sports Science, CAV-Federal University of Pernambuco, Vitoria de Santo Antão, PE, Brazil.
authors.forename: José Luiz
authors.initials: JL
authors.lastname: de Brito Alves
authors.affiliation: Laboratory of Biochemistry and Exercise Biochemistry, CAV-Federal University of Pernambuco, Vitoria de Santo Antão, PE, Brazil; Neuropsychiatry and Behavior Science Graduate Program, Federal University of Pernambuco, 50670-901, Brazil.
authors.forename: Aline Isabel
authors.initials: AI
authors.lastname: da Silva
authors.affiliation: Department of Physical Education and Sports Science, CAV-Federal University of Pernambuco, Vitoria de Santo Antão, PE, Brazil.
authors.forename: João Henrique
authors.initials: JH
authors.lastname: Costa-Silva
authors.affiliation: Laboratory of Biochemistry and Exercise Biochemistry, CAV-Federal University of Pernambuco, Vitoria de Santo Antão, PE, Brazil; Department of Physical Education and Sports Science, CAV-Federal University of Pernambuco, Vitoria de Santo Antão, PE, Brazil; Neuropsychiatry and Behavior Science Graduate Program, Federal University of Pernambuco, 50670-901, Brazil. Electronic address: lagranha@hotmail.com.
authors.forename: Claudia J
authors.initials: CJ
authors.lastname: Lagranha
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002374:Catalase / Q000378:metabolism; D005260:Female; D005473:Fluoxetine / Q000008:administration & dosage / Q000494:pharmacology*; D005978:Glutathione / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D006321:Heart / Q000187:drug effects / Q000502:physiology*; D006439:Hemodynamics / Q000187:drug effects; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D017208:Rats, Wistar; D012701:Serotonin / Q000378:metabolism*; D017367:Selective Serotonin Reuptake Inhibitors / Q000008:administration & dosage / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D002374:Catalase; D005473:Fluoxetine; D005978:Glutathione; D005982:Glutathione Transferase; D008315:Malondialdehyde; D012701:Serotonin; D017367:Selective Serotonin Reuptake Inhibitors; D013482:Superoxide Dismutase
keywords: Brainstem; Heart; Hemodynamic evaluation; Oxidative stress; Serotonin modulation