title: Bacillus zhangzhouensissp. nov. andBacillus australimarissp. nov.
abstract: Two strains, designated DW5-4Tand NH7I_1T, were isolated from aquaculture water of a shrimp farm of Zhangzhou city in China and from surface sediment of the South China Sea, respectively. Cells of the strains were Gram-stain-positive, oxidase- and catalase-positive, rod-shaped and motile. Phylogenetic analyses based on 16S rRNA gene sequences indicated that the two strains belonged to theBacillus pumilusclade. 16S rRNA gene sequence similarity between strains DW5-4Tand NH7I_1Twas 99.8 %, and between each of the two strains and four type strains,B. pumilusATCC 7061T,Bacillus safensisFO-36bT,Bacillus altitudinis41KF2bTandBacillus xiamenensisHYC-10T, were above 99.4 %. The digital DNA-DNA hybridization value between the two novel strains was 41.6 %, and between each of the two strains and the four above-mentioned type strains were below 53.0 %. In both strains, the principal fatty acids were iso-C15 : 0and anteiso-C15 : 0, and the isoprenoid quinone was identified as MK-7 (100 %). The DNA G+C contents of strains DW5-4Tand NH7I_1Twere 41.4 and 41.3 mol%, respectively. Based on phenotypic and genotypic characteristics, two novel species of the genusBacillusare proposed:Bacillus zhangzhouensissp. nov., with DW5-4T( = MCCC 1A08372T= LMG 27144T= KCTC 33531T) as the type strain, andBacillus australimarissp. nov., with NH7I_1T( = MCCC 1A05787T= LMG 27697T= KCTC 33532T) as the type strain.
authors.affiliation: State Key Laboratory Breeding Base of Marine Genetic Resources; Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, SOA; Collaborative Innovation Center of Deep Sea Biology; Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources; Key Laboratory of Marine Genetic Resources of Fujian Province, Xiamen 361005, PRChina.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: State Key Laboratory Breeding Base of Marine Genetic Resources; Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, SOA; Collaborative Innovation Center of Deep Sea Biology; Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources; Key Laboratory of Marine Genetic Resources of Fujian Province, Xiamen 361005, PRChina.
authors.forename: Qiliang
authors.initials: Q
authors.lastname: Lai
authors.affiliation: State Key Laboratory Breeding Base of Marine Genetic Resources; Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, SOA; Collaborative Innovation Center of Deep Sea Biology; Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources; Key Laboratory of Marine Genetic Resources of Fujian Province, Xiamen 361005, PRChina.
authors.forename: Juan
authors.initials: J
authors.lastname: Du
authors.affiliation: State Key Laboratory Breeding Base of Marine Genetic Resources; Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, SOA; Collaborative Innovation Center of Deep Sea Biology; Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources; Key Laboratory of Marine Genetic Resources of Fujian Province, Xiamen 361005, PRChina.
authors.forename: Zongze
authors.initials: Z
authors.lastname: Shao