title: Expression of Cell Surface Receptors on Human Glioblastoma Xenograft Model in NOD/SCID Mouse.
abstract: OBJECTIVE
  To obtain basic data for development of a glioblastoma-specific immunotoxin, the expression of variable cell surface receptors on a human glioblastoma xenograft model was evaluated, using NOD/SCID mice.
  
  
  METHODS
  We developed a xenograft model in NOD/SCID mice implanted with a human glioblastoma cell line (U-87MG). Immunohistochemical studies were performed on implanted tumor nodules (n=8) using antibodies against CD71, EGFR, IGF-IRalpha, CXCR4 and IL-4Ralpha.
  
  
  RESULTS
  Expression of IL-4Ralpha, in implanted tumornodules, was the highest of the cell surface receptors evaluated in this study. However, the endothelial cells in, and around, the tumor nodules also revealed immunopositivity against IL-4Ralpha. The immunoreactivity of IL-4Ralpha, and other surface receptors such as CD71, IGF-IRalpha and EGFR, was prominent in tumor nodules associated with tumor necrosis.
  
  
  CONCLUSIONS
  IL-4Ralpha would be a possible target for the development of glioblastoma-specific immunotoxin, although there are limitations due to its endothelial expression.
authors.forename: Kyung Seung
authors.initials: KS
authors.lastname: Oh
authors.forename: Ki Uk
authors.initials: KU
authors.lastname: Kim
authors.forename: Na Hee
authors.initials: NH
authors.lastname: Park
authors.forename: Su Yeong
authors.initials: SY
authors.lastname: Seo
authors.forename: Sun Seob
authors.initials: SS
authors.lastname: Choi
authors.forename: Gi Yeong
authors.initials: GY
authors.lastname: Huh
keywords: Glioblastoma; Interleukin-4 receptor; SCID mouse