title: Intravital Multiphoton Imaging of the Kidney: Tubular Structure and Metabolism.
abstract: Multiphoton microscopy (MPM) allows the visualization of dynamic pathophysiological events in real time in live animals. Intravital imaging can be applied to investigate novel mechanisms and treatments of different forms of kidney disease as well as improve our understanding of normal kidney physiology. Using rodent models, in conjunction with endogenous fluorescence and infused exogenous fluorescent dyes, measurement can be made of renal processes such as glomerular permeability, juxtaglomerular apparatus function, interactions of the tubulointerstitium, tubulovascular interactions, vascular flow rate, and the renin-angiotensin-aldosterone system. Subcellular processes including mitochondrial dynamics, reactive oxygen species production, cytosolic ion concentrations, and death processes of apoptosis and necrosis can also be seen and measured by MPM. The current methods chapter presents an overview of MPM with a focus on techniques for intravital kidney imaging and gives examples of instances where intravital MPM has been utilized to study renal pathophysiology. Suggestions are provided for MPM methods within the confines of intravital microscopy and selected kidney structure. MPM is undoubtedly a powerful new technique for application in experimental nephrology, and we believe it will continue to create new paradigms for understanding and treating kidney disease.
authors.affiliation: Centre for Kidney Disease Research, School of Medicine, Translational Research Institute, The University of Queensland, 37 Kent Street, Woolloongabba, Brisbane, QLD, 4102, Australia.
authors.forename: David M
authors.initials: DM
authors.lastname: Small
authors.affiliation: Therapeutics Research Centre, School of Medicine, Translational Research Institute, The University of Queensland, Brisbane, QLD, Australia.
authors.forename: Washington Y
authors.initials: WY
authors.lastname: Sanchez
authors.affiliation: Centre for Kidney Disease Research, School of Medicine, Translational Research Institute, The University of Queensland, 37 Kent Street, Woolloongabba, Brisbane, QLD, 4102, Australia. g.gobe@uq.edu.au.
authors.forename: Glenda C
authors.initials: GC
authors.lastname: Gobe
mesh_terms: D058186:Acute Kidney Injury / Q000209:etiology / Q000378:metabolism / Q000473:pathology; D000818:Animals; D004195:Disease Models, Animal; D007668:Kidney / Q000166:cytology* / Q000378:metabolism*; D007677:Kidney Function Tests; D007684:Kidney Tubules / Q000166:cytology / Q000378:metabolism; D008297:Male; D051379:Mice; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods; D008928:Mitochondria / Q000378:metabolism; D015427:Reperfusion Injury / Q000378:metabolism / Q000473:pathology
keywords: Fluorescence; Intravital imaging; Metabolism; Multiphoton; Two-photon excitation