title: Biofunctionalization of Selenium Nanoparticle with Dictyophora Indusiata Polysaccharide and Its Antiproliferative Activity through Death-Receptor and Mitochondria-Mediated Apoptotic Pathways.
abstract: Bio-functionalized nanoparticles with semiconducting/metallic core encapsulated in a bio- or bio-derived materials are promising for applications in biology and especially in cancer diagnostic and healing. In this report, we report a facile, single-step, first-time synthesis and in-situ functionalization strategy for the preparation of monodispersed selenium nanoparticles (SeNPs) functionalized using a novel polysaccharide (DP1) extracted from Dictyophora indusiata (a fungus). The DP1 functionalized SeNPs (DP1-SeNPs), where DP1 is attached to the surface via Se-O bond as well as physic-sorption had, an average diameter of 89 nm, and were highly uniform, extremely stable compared to bare SeNPs. Detailed investigation of the biological properties of DP1-SeNP illustrated that they exhibit unprecedented, enhanced, and selective antiproliferative activity through inducing cell apoptosis confirmed by nuclear condensation, DNA cleavage, and accumulation of S phase cell arrest. The mechanism of the induced apoptosis was found to be a combination of the activation of caspases 3, 8, and 9, the Fas-associated death domain protein (FADD), reactive oxygen species (ROS) overproduction, as well as mitochondrial dysfunction. It is envisioned that the reported DP1-SeNPs will offer a new phase space for high-efficiency anticancer treatment with little side effect.
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, Guangdong, China.
authors.forename: Wenzhen
authors.initials: W
authors.lastname: Liao
authors.affiliation: School of Pharmaceutical Sciences, Guangdong Provincial Key Laboratory of New Drug Screening, Southern Medical University, Guangzhou, Guangdong 510515, China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Yu
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, Guangdong, China.
authors.forename: Zehua
authors.initials: Z
authors.lastname: Lin
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, Guangdong, China.
authors.forename: Zhuogui
authors.initials: Z
authors.lastname: Lei
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, Guangdong, China.
authors.forename: Zhengxiang
authors.initials: Z
authors.lastname: Ning
authors.affiliation: Department of Food Science, Cornell University, Ithaca, NY, 14853-7201, USA.
authors.forename: Joe M
authors.initials: JM
authors.lastname: Regenstein
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, Guangdong, China.
authors.forename: Jiguo
authors.initials: J
authors.lastname: Yang
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, Guangdong, China.|Department of Food Science, Cornell University, Ithaca, NY, 14853-7201, USA.
authors.forename: Jiaoyan
authors.initials: J
authors.lastname: Ren
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D001487:Basidiomycota / Q000737:chemistry; D049109:Cell Proliferation / Q000187:drug effects*; D016503:Drug Delivery Systems; D056945:Hep G2 Cells; D006801:Humans; D008928:Mitochondria / Q000187:drug effects / Q000473:pathology; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D009369:Neoplasms / Q000188:drug therapy / Q000473:pathology; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D053218:Receptors, Death Domain / Q000187:drug effects; D016196:S Phase / Q000187:drug effects; D012643:Selenium / Q000737:chemistry / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D011134:Polysaccharides; D017382:Reactive Oxygen Species; D053218:Receptors, Death Domain; D012643:Selenium