title: [Expression of Twist and relation with epithelial-mesenchymal transition in oral squamous cell carcinoma].
abstract: OBJECTIVE
  The objective of this paper was to study the expression of related protein and Twist transcription factor of epithelial-mesenchymal transition in oral squamous cell carcinoma (OSCC) tissue and the correlations of OSCC and oral squamous cell carcino-metastasis. The paper also investigated the clinical significance of expression on OSCC.
  
  
  METHODS
  The labels of epithelium materialization (E-cadherin and cytokeratin), stromal labels (N-cadherin), transcription factor Twist protein, and mRNA expression in 30 OSCC tissues were investigated via immunohistochemistry and in situ hybridization. The paper also conducted contrast analysis with clinicopathology.
  
  
  RESULTS
  Immunization result showed that the expressions of Twist and N-cadherin in the OSCC group were more significant than those of the normal group (P<0.05). The expressions of E-cadherin and keratin in OSCC were significantly lower than those of the normal group (P<0.05). In the moderate- and low-differentiated group of OSCC, the expressions of Twist and N-cadherin were higher than those of the high-differentiated group (P<0.05). The expressions of E-cadherin and keratin were lower than those in the high-differentiated group (P<0.05). In the lymphatic metastasis group, the expressions of Twist and N-cadherin were higher than those of no-lymphatic metastasis group (P<0.05). The expressions of E-cadherin and keratin were lower than those of the no-lymphatic metastasis group (P< 0.05). Results of in situ hybridization showed that the expression of Twist mRNA in the moderate- and low-differentiated groups of OSCC, T3, and T4 groups as well as that of the lymphatic metastasis group were higher than those of the high-differentiated, T1 and T2 groups, and no-separate lymphatic metastasis group, and the differences were statistically significant (P<0.05).
  
  
  CONCLUSIONS
  Epithelium materialization exists in OSCC tissue. Twist can enhance the invasiveness of tumor cell and promote the infiltration and metastasis of OSCC. The combined detection of Twist, E-cadherin, and N-cadherin expressions can effectively predict and estimate OSCC metastasis.
authors.forename: Hao-xuan
authors.initials: HX
authors.lastname: Sun
authors.forename: Hongchao
authors.initials: H
authors.lastname: Feng
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Song
mesh_terms: D015820:Cadherins; D002294:Carcinoma, Squamous Cell / Q000378:metabolism*; D004847:Epithelial Cells; D058750:Epithelial-Mesenchymal Transition / Q000502:physiology*; D004848:Epithelium; D006801:Humans; D007150:Immunohistochemistry; D008207:Lymphatic Metastasis; D009062:Mouth Neoplasms / Q000378:metabolism*; D012333:RNA, Messenger; D051879:Twist-Related Protein 1 / Q000378:metabolism*
chemical_list: D015820:Cadherins; D012333:RNA, Messenger; D051879:Twist-Related Protein 1