title: Fluorescent in situ hybridization for the localization of viruses, bacteria and other microorganisms in insect and plant tissues.
abstract: Methods for the localization of cellular components such as nucleic acids, proteins, cellular vesicles and more, and the localization of microorganisms including viruses, bacteria and fungi have become an important part of any research program in biological sciences that enable the visualization of these components in fixed and live tissues without the need for complex processing steps. The rapid development of microscopy tools and technologies as well as related fluorescent markers and fluorophores for many cellular components, and the ability to design DNA and RNA sequence-based molecular probes and antibodies which can be visualized fluorescently, have rapidly advanced this field. This review will focus on some of the localizations methods which have been used in plants and insect pests in agriculture, and other microorganisms, which are rapidly advancing the research in agriculture-related fields.
authors.affiliation: Department of Entomology, The Volcani Center, Bet Dagan 50250, Israel.
authors.forename: Adi
authors.initials: A
authors.lastname: Kliot
authors.affiliation: Department of Entomology, The Volcani Center, Bet Dagan 50250, Israel. Electronic address: ghanim@agri.gov.il.
authors.forename: Murad
authors.initials: M
authors.lastname: Ghanim
mesh_terms: D000818:Animals; D020171:Botrytis / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure*; D004076:Digoxigenin / Q000737:chemistry; D004175:Diptera / Q000382:microbiology / Q000648:ultrastructure* / Q000821:virology; D005456:Fluorescent Dyes / Q000737:chemistry; D005786:Gene Expression Regulation; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D009841:Oligonucleotides / Q000737:chemistry; D018532:Pisum sativum / Q000382:microbiology / Q000648:ultrastructure* / Q000821:virology; D012333:RNA, Messenger / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D059629:Signal-To-Noise Ratio; D013559:Symbiosis; D016707:Tissue Fixation / Q000379:methods; D014158:Transcription, Genetic; D048889:Weevils / Q000382:microbiology / Q000648:ultrastructure* / Q000821:virology; D020577:Wolbachia / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D005456:Fluorescent Dyes; D009841:Oligonucleotides; D012333:RNA, Messenger; D004076:Digoxigenin
keywords: Bacteria; Fluorescent in situ hybridization; Localization; Plant virus; Symbiont