title: Reversible immobilization of laccase onto metal-ion-chelated magnetic microspheres for bisphenol A removal.
abstract: Increasing attention has been given to nanobiocatalysis for commercial applications. In this study, laccase was reversibly immobilized onto Cu(ΙΙ)- and Mn(ΙΙ)-chelated magnetic microspheres and successfully applied to remove bisphenol A (BPA) from water. The results indicated that the loading of laccase onto the metal-ion-chelated magnetic microspheres was approximately 100mg/g. After five successive adsorption-desorption cycles, the laccase adsorption capacities did not change. In comparison with free laccase, the thermal and storage stabilities of immobilized laccase were significantly improved. Immobilized laccase exhibited a high removal efficiency for BPA under the combined actions of biodegradation and adsorption. Greater than 85% of BPA was removed under optimum conditions. The effects of various factors on the BPA removal efficiency of immobilized laccase were analysed. The results showed that metal-ion-chelated magnetic microspheres have great potential for industrial applications.
authors.affiliation: Institute of Biomaterial, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Jiahong
authors.initials: J
authors.lastname: Lin
authors.affiliation: Institute of Biomaterial, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Yingju
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Institute of Biomaterial, South China Agricultural University, Guangzhou 510642, China. Electronic address: chens1968@hotmail.com.
authors.forename: Shi
authors.initials: S
authors.lastname: Chen
authors.affiliation: Institute of Biomaterial, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Xueyi
authors.initials: X
authors.lastname: Le
authors.affiliation: Institute of Biomaterial, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Institute of Biomaterial, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: Institute of Biomaterial, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Yiyi
authors.initials: Y
authors.lastname: Ou
authors.affiliation: Institute of Biomaterial, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Yang
mesh_terms: D000327:Adsorption; D001559:Benzhydryl Compounds / Q000737:chemistry*; D004795:Enzyme Stability; D004800:Enzymes, Immobilized; D005290:Ferric Compounds / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007477:Ions / Q000737:chemistry*; D007700:Kinetics; D042845:Laccase / Q000737:chemistry*; D059346:Magnets; D008670:Metals / Q000737:chemistry*; D008863:Microspheres; D010316:Particle Size; D010636:Phenols / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D004800:Enzymes, Immobilized; D005290:Ferric Compounds; D007477:Ions; D042845:Laccase; D008670:Metals; D010636:Phenols; C000499:ferric oxide; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: Bisphenol A; Immobilized laccase; Removal