title: Fabrication and characterization of a novel carbon fiber-reinforced calcium phosphate silicate bone cement with potential osteo-inductivity.
abstract: The repair of bone defects is still a pressing challenge in clinics. Injectable bone cement is regarded as a promising material to solve this problem because of its special self-setting property. Unfortunately, its poor mechanical conformability, unfavorable osteo-genesis ability and insufficient osteo-inductivity seriously limit its clinical application. In this study, novel experimental calcium phosphate silicate bone cement reinforced by carbon fibers (CCPSC) was fabricated and characterized. First, a compressive strength test and cell culture study were carried out. Then, the material was implanted into the femoral epiphysis of beagle dogs to further assess its osteo-conductivity using a micro-computed tomography scan and histological analysis. In addition, we implanted CCPSC into the beagles' intramuscular pouches to perform an elementary investigation of its osteo-inductivity. The results showed that incorporation of carbon fibers significantly improved its mechanical properties. Meanwhile, CCPSC had better biocompatibility to activate cell adhesion as well as proliferation than poly-methyl methacrylate bone cement based on the cell culture study. Moreover, pronounced biodegradability and improved osteo-conductivity of CCPSC could be observed through the in vivo animal study. Finally, a small amount of osteoid was found at the heterotopic site one month after implantation which indicated potential osteo-inductivity of CCPSC. In conclusion, the novel CCPSC shows promise as a bioactive bone substitute in certain load-bearing circumstances.
authors.affiliation: State Key Laboratory of Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu, Sichuan 610041, People's Republic of China.
authors.forename: Jiangjiang
authors.initials: J
authors.lastname: Zheng
authors.forename: Yu
authors.initials: Y
authors.lastname: Xiao
authors.forename: Tianxing
authors.initials: T
authors.lastname: Gong
authors.forename: Shuxin
authors.initials: S
authors.lastname: Zhou
authors.forename: Tom
authors.initials: T
authors.lastname: Troczynski
authors.forename: Quanzu
authors.initials: Q
authors.lastname: Yang
authors.forename: Chongyun
authors.initials: C
authors.lastname: Bao
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Xu
mesh_terms: D000818:Animals; D001843:Bone Cements / Q000138:chemical synthesis* / Q000627:therapeutic use*; D002130:Calcium Phosphates / Q000737:chemistry*; D002244:Carbon / Q000737:chemistry*; D000077482:Carbon Fiber; D002478:Cells, Cultured; D019245:Compressive Strength; D004285:Dogs; D005264:Femoral Fractures / Q000473:pathology / Q000628:therapy*; D008297:Male; D008422:Materials Testing; D010012:Osteogenesis / Q000502:physiology*; D019904:Polymethyl Methacrylate / Q000737:chemistry; D017640:Silicates / Q000737:chemistry / Q000627:therapeutic use; D016896:Treatment Outcome
chemical_list: D001843:Bone Cements; D002130:Calcium Phosphates; D000077482:Carbon Fiber; D017640:Silicates; D002244:Carbon; D019904:Polymethyl Methacrylate; C020243:calcium phosphate