title: Investigation of the impact of antinuclear antibody on the outcome of in vitro fertilization/intracytoplasmic sperm injection treatment.
abstract: OBJECTIVE
  The aim of this study is to investigate the influence of antinuclear antibodies (ANAs) on the pregnancy and early miscarriage rates, thereby evaluating the outcome of in vitro fertilization and intracytoplasmic sperm injection (IVF/ICSI) treatment.
  
  
  METHODS
  A total of 517 infertile female patients undergoing IVF/ICSI treatment (experimental group) were chosen for this study, and 186 women with normal reproductive history (control group) were designated as the control. Serum ANAs from the participants were tested using indirect immunofluorescence assay, while antiextractable nuclear antigens were tested by immune blot assay.
  
  
  RESULTS
  The ANA expression in the infertile patients (39.45%) was higher than that in the control group (16.13%). A high ANA titer (≥1:320) was found only in infertile patients. ANA positivity significantly decreased the pregnancy rate and increased the early miscarriage rate after IVF/ICSI treatment. The rate of early miscarriage was higher in the high-ANA-titer individuals after IVF/ICSI treatment. Clinical pregnancy rate in anti-scl-70- and anti-PM-scl-positive individuals after IVF/ICSI treatment was lower than that in the ANA-negative individuals. Anti-Rib-p, anti-Jo-1, and anti-dsDNA were found to cause high risk of early miscarriage in pregnant women.
  
  
  CONCLUSIONS
  ANA positivity may not only be the cause of bad outcome during IVF/ICSI treatment, but also pose as a risk factor for IVF/ICSI treatment.
authors.affiliation: Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.affiliation: Beijing Youan Hospital, Capital Medical University, Beijing, China.
authors.forename: Yipeng
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing, China.
authors.forename: Yanmin
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing, China.
authors.forename: Yonglian
authors.initials: Y
authors.lastname: Lan
authors.affiliation: Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing, China.
authors.forename: Chanwei
authors.initials: C
authors.lastname: Jia
authors.affiliation: Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Liang
authors.affiliation: Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing, China. Electronic address: yushu572000@126.com.
authors.forename: Shuyu
authors.initials: S
authors.lastname: Wang
mesh_terms: D000022:Abortion, Spontaneous / Q000453:epidemiology*; D000328:Adult; D000974:Antibodies, Antinuclear / Q000097:blood*; D016022:Case-Control Studies; D002681:China / Q000453:epidemiology; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D007247:Infertility, Female / Q000628:therapy; D054315:Oocyte Retrieval / Q000706:statistics & numerical data; D011247:Pregnancy; D018873:Pregnancy Rate; D012269:Ribosomal Proteins / Q000276:immunology; D020554:Sperm Injections, Intracytoplasmic
chemical_list: D000974:Antibodies, Antinuclear; D012269:Ribosomal Proteins
keywords: antinuclear antibodies; immune blot assay; indirect immunofluorescence assay; infertility; in vitro fertilization/intracytoplasmic sperm injection