title: Establishment of Diagnostic Doses of Five Pyrethroids for Monitoring Physiological Resistance in Aedes Albopictus in Thailand.
abstract: Monitoring insecticide resistance of Aedes albopictus is required for implementing effective dengue and chikungunya vector control in Thailand. The World Health Organization standard susceptibility test for adult mosquitoes was used to determine the baseline susceptibility of a pyrethroid-susceptible laboratory strain of Ae. albopictus to 5 different pyrethroids (deltamethrin, permethrin, bifenthrin, cypermethrin, and α-cypermethrin). Subsequently, the respective established diagnostic doses (0.026% deltamethrin, 1.024% permethrin, 0.570% bifenthrin, 0.237% cypermethrin, and 0.035% α-cypermethrin) were used to test field-collected Ae. albopictus from Rayong, Koh Chang, and Pong Nom Ron. As expected, the laboratory strain was completely susceptible to all pyrethroid insecticides at the established concentrations. Rayong mosquitoes were found to be highly susceptible to bifenthrin, cypermethrin, and α-cypermethrin. Koh Chang mosquitoes were susceptible to only deltamethrin and permethrin. Pong Nom Ron mosquitoes were resistant to all pyrethroids tested. Routine assessment of these baseline results should guide future resistance monitoring to pyrethroid insecticides in Ae. albopictus in Thailand.
authors.affiliation: 1  Bureau of Vector-Borne Diseases, Department of Disease Control, Ministry of Public Health, Nonthaburi 11000, Thailand.
authors.forename: Kanutcharee
authors.initials: K
authors.lastname: Thanispong
authors.affiliation: 2  National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi 11000, Thailand.
authors.forename: Sunaiyana
authors.initials: S
authors.lastname: Sathantriphop
authors.affiliation: 3  Department of Entomology, Faculty of Agriculture, Kasetsart University, Bangkok 10900, Thailand.
authors.forename: Naritsara
authors.initials: N
authors.lastname: Malaithong
authors.affiliation: 3  Department of Entomology, Faculty of Agriculture, Kasetsart University, Bangkok 10900, Thailand.|4  Public Health & Malaria Control Department, International SOS, Kuala Kencana, Papua, 99920, Indonesia.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Bangs
authors.affiliation: 3  Department of Entomology, Faculty of Agriculture, Kasetsart University, Bangkok 10900, Thailand.
authors.forename: Theeraphap
authors.initials: T
authors.lastname: Chareonviriyaphap
mesh_terms: D000330:Aedes / Q000187:drug effects* / Q000235:genetics; D000818:Animals; D004305:Dose-Response Relationship, Drug; D007305:Insecticide Resistance; D007306:Insecticides / Q000494:pharmacology*; D009032:Mosquito Control; D011722:Pyrethrins / Q000494:pharmacology*; D013785:Thailand
chemical_list: D007306:Insecticides; D011722:Pyrethrins
keywords: Aedes albopictus; diagnostic dose; pyrethroid; susceptibility