title: A novel method for monitoring troponin T fragment from rabbit skeletal muscle during aging using quartz crystal microbalance.
abstract: BACKGROUND
  Troponin T (TnT) is degraded during aging of meat. The proteolytic fragment of TnT, especially the 30 kDa fragment, is used as one of indices for estimating aging of meat. We have tried to use quartz crystal microbalance (QCM), which is widely used to analyze interaction among macromolecules, to detect proteolytic fragments of TnT during aging of meat.
  
  
  RESULTS
  The frequency of the QCM sensor with immobilized anti-TnT antibody in high-salt solution extracts of both myofibrils and whole meat decreased with time of aging. The staining intensity of the bands, including a 30 kDa fragment bound to anti-TnT antibody, also increased with time of aging in western blotting. These results confirm that TnT is degraded during aging and released from thin filaments, and QCM analysis is sufficiently sensitive to detect the TnT fragments.
  
  
  CONCLUSIONS
  The QCM analysis of muscle and myofibrillar extracts using anti-TnT antibody-immobilized sensor can be used as a convenient tool for monitoring the extent of aging of meat. © 2015 Society of Chemical Industry.
authors.affiliation: Department of Food Science and Human Wellness, Rakuno Gakuen University, Hokkaido, Japan.
authors.forename: Tomohito
authors.initials: T
authors.lastname: Iwasaki
authors.affiliation: Department of Food Science and Human Wellness, Rakuno Gakuen University, Hokkaido, Japan.
authors.forename: Hironobu
authors.initials: H
authors.lastname: Taniguchi
authors.affiliation: Department of Food Science and Human Wellness, Rakuno Gakuen University, Hokkaido, Japan.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Hasegawa
authors.affiliation: Safety Research Institute for Chemical Compounds Co. Ltd, Hokkaido, Japan.
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Maeda
authors.affiliation: Department of Food Science and Human Wellness, Rakuno Gakuen University, Hokkaido, Japan.
authors.forename: Katsuhiro
authors.initials: K
authors.lastname: Yamamoto
mesh_terms: D000818:Animals; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D063427:Food Quality; D061353:Food Storage; D047468:Immunoprecipitation; D007564:Japan; D008460:Meat / Q000032:analysis*; D008970:Molecular Weight; D018482:Muscle, Skeletal / Q000201:enzymology / Q000378:metabolism*; D009210:Myofibrils / Q000201:enzymology / Q000378:metabolism*; D010446:Peptide Fragments / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D010449:Peptide Mapping; D059748:Proteolysis; D058525:Quartz Crystal Microbalance Techniques; D011817:Rabbits; D020107:Troponin T / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism
chemical_list: D010446:Peptide Fragments; D020107:Troponin T
keywords: meat aging; quartz crystal microbalance; troponin T