title: Compact Wireless Microscope for In-Situ Time Course Study of Large Scale Cell Dynamics within an Incubator.
abstract: Imaging of live cells in a region of interest is essential to life science research. Unlike the traditional way that mounts CO2 incubator onto a bulky microscope for observation, here we propose a wireless microscope (termed w-SCOPE) that is based on the "microscope-in-incubator" concept and can be easily housed into a standard CO2 incubator for prolonged on-site observation of the cells. The w-SCOPE is capable of tunable magnification, remote control and wireless image transmission. At the same time, it is compact, measuring only ~10 cm in each dimension, and cost-effective. With the enhancement of compressive sensing computation, the acquired images can achieve a wide field of view (FOV) of ~113 mm(2) as well as a cellular resolution of ~3 μm, which enables various forms of follow-up image-based cell analysis. We performed 12 hours time-lapse study on paclitaxel-treated MCF-7 and HEK293T cell lines using w-SCOPE. The analytic results, such as the calculated viability and therapeutic window, from our device were validated by standard cell detection assays and imaging-based cytometer. In addition to those end-point detection methods, w-SCOPE further uncovered the time course of the cell's response to the drug treatment over the whole period of drug exposure.
authors.affiliation: Department of Precision Instrument, Tsinghua University, Beijing, 100084, China.
authors.forename: Di
authors.initials: D
authors.lastname: Jin
authors.affiliation: Department of Bioengineering, University of California, Los Angeles, Los Angeles, 90095, U.S.A.
authors.forename: Dennis
authors.initials: D
authors.lastname: Wong
authors.affiliation: School of Life Sciences, Tsinghua University, Beijing, 100084, China.
authors.forename: Junxiang
authors.initials: J
authors.lastname: Li
authors.affiliation: School of Mechanical Science and Engineering, Huazhong University of Science and Technology, Wuhan, 430074, China.
authors.forename: Zhang
authors.initials: Z
authors.lastname: Luo
authors.affiliation: College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, China.
authors.forename: Yiran
authors.initials: Y
authors.lastname: Guo
authors.affiliation: College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, China.
authors.forename: Bifeng
authors.initials: B
authors.lastname: Liu
authors.affiliation: School of Life Sciences, Tsinghua University, Beijing, 100084, China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Wu
authors.affiliation: Mechanical and Aerospace Engineering Department, University of California, Los Angeles, Los Angeles, 90095, U.S.A.
authors.forename: Chih-Ming
authors.initials: CM
authors.lastname: Ho
authors.affiliation: School of Optical and Electronic Information, Huazhong University of Science and Technology, Wuhan, 430074, China.|Mechanical and Aerospace Engineering Department, University of California, Los Angeles, Los Angeles, 90095, U.S.A.
authors.forename: Peng
authors.initials: P
authors.lastname: Fei
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D001331:Automation; D002452:Cell Count / Q000379:methods; D002470:Cell Survival / Q000187:drug effects; D004867:Equipment Design; D057809:HEK293 Cells; D006801:Humans; D007185:Incubators; D061986:MCF-7 Cells; D008853:Microscopy / Q000295:instrumentation / Q000379:methods*; D017239:Paclitaxel / Q000494:pharmacology; D059015:Wireless Technology
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D017239:Paclitaxel