title: Antibodies against neo-epitope tTg complexed to gliadin are different and more reliable then anti-tTg for the diagnosis of pediatric celiac disease.
abstract: The neo-epitope tTg (tTg-neo) autoantibody, never challenged the anti-tissue transglutaminase (tTg) premiership, recommended by ESPGHAN, for celiac disease (CD) diagnosis. Pediatric CD (PCD), abdominal pains and normal children, normal adults, and rheumatoid arthritis patients, were tested using the following ELISAs detecting IgA, IgG or both IgA and IgG (check): AESKULISA® tTg (tTg; RUO) and AESKULISA® tTg-neo. Higher OD activity was detected for tTg-neo IgA, IgG and IgA+IgG than for tTg. tTg-neo IgA, IgG correlated better with intestinal damage than tTg. The tTg-neo combined IgA+IgG ELISA kit had higher sensitivity and a comparable specificity for the diagnosis of PCD. The drop in the % competition was much higher with the tTg-neo then the tTg antibodies. The false positivity of the tTg was significantly higher than the tTg-neo one. Serological diagnostic performances, reflection of intestinal damage, diverse epitopes and false positivity were better with the tTg-neo.
authors.affiliation: AESKU.KIPP Institute, Mikroforum Ring 2, Wendelsheim 55234, Germany; B. Rappaport School of Medicine, Technion-Israel Institute of Technology, Bat Galim, Haifa, Israel. Electronic address: aaronlerner1948@gmail.com.
authors.forename: Aaron
authors.initials: A
authors.lastname: Lerner
authors.affiliation: AESKU.KIPP Institute, Mikroforum Ring 2, Wendelsheim 55234, Germany.
authors.forename: Patricia
authors.initials: P
authors.lastname: Jeremias
authors.affiliation: AESKU.KIPP Institute, Mikroforum Ring 2, Wendelsheim 55234, Germany.
authors.forename: Sandra
authors.initials: S
authors.lastname: Neidhöfer
authors.affiliation: AESKU.KIPP Institute, Mikroforum Ring 2, Wendelsheim 55234, Germany. Electronic address: matthias@aesku.com.
authors.forename: Torsten
authors.initials: T
authors.lastname: Matthias
mesh_terms: D000328:Adult; D000906:Antibodies / Q000276:immunology*; D002446:Celiac Disease / Q000175:diagnosis* / Q000276:immunology*; D002648:Child; D000939:Epitopes / Q000276:immunology*; D005260:Female; D005903:Gliadin / Q000737:chemistry / Q000276:immunology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011503:Transglutaminases / Q000737:chemistry* / Q000276:immunology*
chemical_list: D000906:Antibodies; D000939:Epitopes; D005903:Gliadin; D011503:Transglutaminases
keywords: Antibodies; Autoantibodies; Celiac disease; Neo-epitope tTg; Serological markers; Tissue transglutaminase