title: The role of supplemental ultraviolet-B radiation in altering the metabolite profile, essential oil content and composition, and free radical scavenging activities of Coleus forskohlii, an indigenous medicinal plant.
abstract: The effects of supplemental ultraviolet-B (s-UV-B; 3.6 kJ m(-2) day(-1) above ambient) radiation were investigated on plant metabolite profile, essential oil content and composition, and free radical scavenging capacities of methanolic extracts of Coleus forskohlii (an indigenous medicinal plant) grown under field conditions. Essential oil was isolated using hydrodistillation technique while alterations in metabolite profile and oil composition were determined via gas chromatography-mass spectroscopy (GC-MS). Leaf and root methanolic extracts were investigated via various in vitro assays for their DPPH radical-, superoxide radical-, hydrogen peroxide-, hydroxyl radical-, and nitric oxide radical scavenging activities, ferrous ion chelating activity, and reducing power. Phytochemical analysis revealed the presence of alkaloids, anthocyanins, coumarins, flavonoids, glycosides, phenols, saponins, steroids, tannins, and terpenoids. Oil content was found to be reduced (by ∼7 %) in supplemental UV-B (s-UV-B) treated plants; the composition of the plant extracts as well as essential oil was also considerably altered. Methanolic extracts from treated plant organs showed more potency as free radical scavengers (their EC50 values being lower than their respective controls). Anomalies were observed in Fe(2+) chelating activity for both leaves and roots. The present study concludes that s-UV-B adversely affects oil content in C. forskohlii and also alters the composition and contents of metabolites in both plant extracts and oil. The results also denote that s-UV-B treated plant organs might be more effective in safeguarding against oxidative stress, though further studies are required to authenticate these findings.
authors.affiliation: Laboratory of Air Pollution and Global Climate Change, Department of Botany, Banaras Hindu University, Varanasi, 221 005, India.
authors.forename: Swabha
authors.initials: S
authors.lastname: Takshak
authors.affiliation: Laboratory of Air Pollution and Global Climate Change, Department of Botany, Banaras Hindu University, Varanasi, 221 005, India. sbagrawal56@gmail.com.
authors.forename: S B
authors.initials: SB
authors.lastname: Agrawal
mesh_terms: D016166:Free Radical Scavengers / Q000378:metabolism* / Q000528:radiation effects; D008401:Gas Chromatography-Mass Spectrometry; D007194:India; D009822:Oils, Volatile / Q000032:analysis* / Q000528:radiation effects; D010936:Plant Extracts / Q000032:analysis* / Q000528:radiation effects; D018515:Plant Leaves / Q000737:chemistry / Q000378:metabolism / Q000528:radiation effects; D018517:Plant Roots / Q000737:chemistry / Q000378:metabolism / Q000528:radiation effects; D010946:Plants, Medicinal / Q000378:metabolism / Q000528:radiation effects; D031346:Plectranthus / Q000378:metabolism* / Q000528:radiation effects; D014466:Ultraviolet Rays / Q000009:adverse effects*
chemical_list: D016166:Free Radical Scavengers; D009822:Oils, Volatile; D010936:Plant Extracts
keywords: Coleus forskohlii; Essential oil; Free radical scavenging activities; Metabolite profile; Methanolic extracts; s-UV-B