title: Development of a real-time quantitative polymerase chain reaction assay for the detection of congenital human cytomegalovirus infection in urine samples.
abstract: A TaqMan real-time quantitative PCR (qPCR), based on amplification of HCMV UL54 gene specific sequence, was developed and compared with shell vial viral culture assay, the gold standard technique for the diagnosis of congenital HCMV infection, using urine samples collected from 110 newborns. The results indicate that this qPCR is slightly more sensitive than shell vial assay suggesting that qPCR may be considered a useful alternative for diagnosing congenital HCMV infection.
authors.affiliation: Microbiology and Virology Unit, Department of Pathology and Diagnostic, University of Verona, Strada Delle Grazie 8, 37134 Verona, Italy. Electronic address: marco.ligozzi@univr.it.
authors.forename: Marco
authors.initials: M
authors.lastname: Ligozzi
authors.affiliation: Microbiology and Virology Unit, Department of Pathology, Azienda Ospedaliera Universitaria Integrata of Verona, Piazzale Stefani 10, 35124 Verona, Italy.
authors.forename: Mara
authors.initials: M
authors.lastname: Poggi
authors.affiliation: Microbiology and Virology Unit, Department of Pathology, Azienda Ospedaliera Universitaria Integrata of Verona, Piazzale Stefani 10, 35124 Verona, Italy.
authors.forename: Marilena
authors.initials: M
authors.lastname: Saletti
authors.affiliation: Microbiology and Virology Unit, Department of Pathology and Diagnostic, University of Verona, Strada Delle Grazie 8, 37134 Verona, Italy.
authors.forename: Davide
authors.initials: D
authors.lastname: Gibellini
mesh_terms: D003587:Cytomegalovirus / Q000235:genetics*; D003586:Cytomegalovirus Infections / Q000151:congenital / Q000175:diagnosis* / Q000652:urine; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics* / Q000652:urine; D006801:Humans; D007231:Infant, Newborn; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA / Q000379:methods
chemical_list: D004279:DNA, Viral
keywords: Congenital infection; HCMV; PCR; Shell vial viral culture