title: Metabolic engineering of long chain-polyunsaturated fatty acid biosynthetic pathway in oleaginous fungus for dihomo-gamma linolenic acid production.
abstract: Microbial lipids are promising alternative sources of long chain-polyunsaturated fatty acids (LC-PUFAs) for food, feed, nutraceutical and pharmaceutical sectors. Dihomo-γ-linolenic acid (C20:3Δ(8,11,14); DGLA) is an important LC-PUFAs with anti-inflammatory and anti-proliferative effects. To generate a DGLA-producing strain, fatty acid reconstitution in Aspergillus oryzae was performed by metabolic engineering through co-expression of codon-optimized Pythium Δ(6)-desaturase and Δ(6)-elongase, which had high conversion rates of substrates to respective products as compared to the native enzymes. The Δ(6)-desaturated and Δ(6)-elongated products, γ-linolenic acid (C18:3Δ(6,9,12); GLA) and DGLA, were accumulated in phospholipids rather than triacylglycerol. Interestingly, the manipulation of lipid quality in the oleaginous fungus did not affect growth and lipid phenotypes. This strategy might expand to development of the oleaginous fungal strain for producing other tailor-made oils with industrial applications.
authors.affiliation: National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency (NSTDA), 113 Thailand Science Park, Phahonyothin Road, Khlong Nueng, Khlong Luang, Pathum Thani 12120, Thailand.
authors.forename: Chanikul
authors.initials: C
authors.lastname: Chutrakul
authors.affiliation: National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency (NSTDA), 113 Thailand Science Park, Phahonyothin Road, Khlong Nueng, Khlong Luang, Pathum Thani 12120, Thailand.
authors.forename: Sukanya
authors.initials: S
authors.lastname: Jeennor
authors.affiliation: National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency (NSTDA), 113 Thailand Science Park, Phahonyothin Road, Khlong Nueng, Khlong Luang, Pathum Thani 12120, Thailand.
authors.forename: Sarocha
authors.initials: S
authors.lastname: Panchanawaporn
authors.affiliation: National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency (NSTDA), 113 Thailand Science Park, Phahonyothin Road, Khlong Nueng, Khlong Luang, Pathum Thani 12120, Thailand.
authors.forename: Pattsarun
authors.initials: P
authors.lastname: Cheawchanlertfa
authors.affiliation: National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency (NSTDA), 113 Thailand Science Park, Phahonyothin Road, Khlong Nueng, Khlong Luang, Pathum Thani 12120, Thailand.
authors.forename: Sarinya
authors.initials: S
authors.lastname: Suttiwattanakul
authors.affiliation: National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency (NSTDA), 113 Thailand Science Park, Phahonyothin Road, Khlong Nueng, Khlong Luang, Pathum Thani 12120, Thailand.
authors.forename: Mayura
authors.initials: M
authors.lastname: Veerana
authors.affiliation: National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency (NSTDA), 113 Thailand Science Park, Phahonyothin Road, Khlong Nueng, Khlong Luang, Pathum Thani 12120, Thailand. Electronic address: laotengk@yahoo.com.
authors.forename: Kobkul
authors.initials: K
authors.lastname: Laoteng
mesh_terms: D015126:8,11,14-Eicosatrienoic Acid / Q000378:metabolism*; D001236:Aspergillus oryzae / Q000235:genetics* / Q000378:metabolism*; D053898:Biosynthetic Pathways; D053938:DNA Fragmentation; D004271:DNA, Fungal / Q000235:genetics / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005231:Fatty Acids, Unsaturated / Q000096:biosynthesis*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D050602:Linoleoyl-CoA Desaturase / Q000235:genetics / Q000378:metabolism; D060847:Metabolic Engineering / Q000379:methods*; D011775:Pythium / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D017965:gamma-Linolenic Acid / Q000096:biosynthesis
chemical_list: D004271:DNA, Fungal; D005231:Fatty Acids, Unsaturated; D005656:Fungal Proteins; D017965:gamma-Linolenic Acid; D050602:Linoleoyl-CoA Desaturase; D015126:8,11,14-Eicosatrienoic Acid
keywords: Aspergillus oryzae; Codon optimization; Dihomo-gamma linolenic acid; Δ(6)-Desaturase; Δ(6)-Elongase