title: Principles of cryo-EM single-particle image processing.
abstract: Single-particle reconstruction is the process by which 3D density maps are obtained from a set of low-dose cryo-EM images of individual macromolecules. This review considers the fundamental principles of this process and the steps in the overall workflow for single-particle image processing. Also considered are the limits that image signal-to-noise ratio places on resolution and the distinguishing of heterogeneous particle populations.
authors.affiliation: Department of Cellular and Molecular Physiology, Yale University, 333 Cedar Street, New Haven, CT 06520, USA Department of Molecular Biophysics and Biochemistry, Yale University, 333 Cedar Street, New Haven, CT 06520, USA fred.sigworth@yale.edu.
authors.forename: Fred J
authors.initials: FJ
authors.lastname: Sigworth
mesh_terms: D020285:Cryoelectron Microscopy / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D046911:Macromolecular Substances / Q000032:analysis*; D011487:Protein Conformation; D059629:Signal-To-Noise Ratio
chemical_list: D046911:Macromolecular Substances
keywords: 3D reconstruction; SNR; noise