title: Lactobacillus plantarum NCIMB8826 ameliorates inflammation of colon and skin in human APOC1 transgenic mice.
abstract: Genetic predisposition and environmental factors, including the gut microbiota, have been suggested as major factors in the development and progression of atopic dermatitis. Hyperlipidemic human APOC1(+/+) transgenic mice display many features of human atopic dermatitis, such as scaling, lichenification, excoriations, and pruritus, along with a disturbed skin barrier function. Cytokine analysis of serum shows an increase of various pro-inflammatory cytokines, including interleukin (IL)-12p40, IL-6, and IL-1α, but lower levels of interferon-γ. These mice also display aspects of colitis evident from macroscopic and histological abnormalities. Genome-wide transcriptome analysis of the intestine shows up-regulation of several genes associated with mast cells and eosinophils and this observation was confirmed by demonstrating increased numbers of IgE(+) and FcRε(+) mast cells in the colon and in the skin. Oral treatment with Lactobacillus plantarum NCIMB8826 resulted in decreased numbers of mast cells in the colon. Moreover, this L. plantarum strain ameliorated skin pathology, evident from improved skin barrier integrity, absence of skin thickening, and less excoriations. These results suggest that modulation of intestinal immune homeostasis contributes to the suppression of atopic dermatitis.
authors.affiliation: 1 TNO, Metabolic Health Research, Zernikedreef 9, 2333 CK Leiden, the Netherlands.|2 Leiden University Medical Centrum, Department of Immunohematology and Bloodtransfusion, Albinusdreef 2, 2333 ZA Leiden, the Netherlands.
authors.forename: R
authors.initials: R
authors.lastname: Mariman
authors.affiliation: 1 TNO, Metabolic Health Research, Zernikedreef 9, 2333 CK Leiden, the Netherlands.
authors.forename: E
authors.initials: E
authors.lastname: Reefman
authors.affiliation: 1 TNO, Metabolic Health Research, Zernikedreef 9, 2333 CK Leiden, the Netherlands.
authors.forename: F
authors.initials: F
authors.lastname: Tielen
authors.affiliation: 1 TNO, Metabolic Health Research, Zernikedreef 9, 2333 CK Leiden, the Netherlands.
authors.forename: C
authors.initials: C
authors.lastname: Persoon-Deen
authors.affiliation: 1 TNO, Metabolic Health Research, Zernikedreef 9, 2333 CK Leiden, the Netherlands.
authors.forename: K
authors.initials: K
authors.lastname: van de Mark
authors.affiliation: 1 TNO, Metabolic Health Research, Zernikedreef 9, 2333 CK Leiden, the Netherlands.
authors.forename: N
authors.initials: N
authors.lastname: Worms
authors.affiliation: 2 Leiden University Medical Centrum, Department of Immunohematology and Bloodtransfusion, Albinusdreef 2, 2333 ZA Leiden, the Netherlands.
authors.forename: F
authors.initials: F
authors.lastname: Koning
authors.affiliation: 1 TNO, Metabolic Health Research, Zernikedreef 9, 2333 CK Leiden, the Netherlands.
authors.forename: L
authors.initials: L
authors.lastname: Nagelkerken
mesh_terms: D000818:Animals; D003106:Colon / Q000187:drug effects / Q000276:immunology*; D003876:Dermatitis, Atopic / Q000188:drug therapy* / Q000235:genetics / Q000276:immunology; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics / Q000276:immunology; D015850:Interleukin-6 / Q000235:genetics / Q000276:immunology; D048191:Lactiplantibacillus plantarum / Q000502:physiology*; D008407:Mast Cells / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D019936:Probiotics / Q000008:administration & dosage*; D012867:Skin / Q000187:drug effects / Q000276:immunology
chemical_list: D015850:Interleukin-6; D007371:Interferon-gamma
keywords: APOC1+/+ transgenic mice model; gene expression profiling; intestine; mast cells; probiotics