title: NAIplot: An opensource web tool to visualize neuraminidase inhibitor (NAI) phenotypic susceptibility results using kernel density plots.
abstract: The results of neuraminidase inhibitor (NAI) enzyme inhibition assays are commonly expressed as 50% inhibitory concentration (IC50) fold-change values and presented graphically in box plots (box-and-whisker plots). An alternative and more informative type of graph is the kernel density plot, which we propose should be the preferred one for this purpose. In this paper we discuss the limitations of box plots and the advantages of the kernel density plot, and we present NAIplot, an opensource web application that allows convenient creation of density plots specifically for visualizing the results of NAI enzyme inhibition assays, as well as for general purposes.
authors.affiliation: Department of Epidemiological Surveillance and Intervention, Hellenic Centre for Disease Control and Prevention, Athens, Greece; Centre for Research in Environmental Epidemiology (CREAL), Barcelona, Spain; Department of Experimental and Health Sciences, Universitat Pompeu Fabra (UPF), Barcelona, Spain. Electronic address: thlytras@gmail.com.
authors.forename: Theodore
authors.initials: T
authors.lastname: Lytras
authors.affiliation: National Influenza Reference Laboratory for Southern Greece, Hellenic Pasteur Institute, Athens, Greece.
authors.forename: Athanasios
authors.initials: A
authors.lastname: Kossyvakis
authors.affiliation: National Influenza Reference Laboratory for Southern Greece, Hellenic Pasteur Institute, Athens, Greece.
authors.forename: Andreas
authors.initials: A
authors.lastname: Mentis
mesh_terms: D000998:Antiviral Agents / Q000494:pharmacology*; D003196:Computer Graphics; D057075:Enzyme Assays; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000187:drug effects / Q000201:enzymology; D009981:Influenza B virus / Q000187:drug effects; D007251:Influenza, Human / Q000188:drug therapy / Q000821:virology; D020128:Inhibitory Concentration 50; D020407:Internet; D008826:Microbial Sensitivity Tests; D009439:Neuraminidase / Q000037:antagonists & inhibitors*; D053139:Oseltamivir / Q000494:pharmacology; D053243:Zanamivir / Q000494:pharmacology
chemical_list: D000998:Antiviral Agents; D004791:Enzyme Inhibitors; D053139:Oseltamivir; D009439:Neuraminidase; D053243:Zanamivir
keywords: Antiviral susceptibility; Bimodality; Box plot; Density plot; Inhibitory concentration; Neuraminidase inhibitor