title: A Novel Anti-Inflammatory Effect for High Density Lipoprotein.
abstract: High density lipoprotein has anti-inflammatory effects in addition to mediating reverse cholesterol transport. While many of the chronic anti-inflammatory effects of high density lipoprotein (HDL) are attributed to changes in cell adhesion molecules, little is known about acute signal transduction events elicited by HDL in endothelial cells. We now show that high density lipoprotein decreases endothelial cell exocytosis, the first step in leukocyte trafficking. ApoA-I, a major apolipoprotein of HDL, mediates inhibition of endothelial cell exocytosis by interacting with endothelial scavenger receptor-BI which triggers an intracellular protective signaling cascade involving protein kinase C (PKC). Other apolipoproteins within the HDL particle have only modest effects upon endothelial exocytosis. Using a human primary culture of endothelial cells and murine apo-AI knockout mice, we show that apo-AI prevents endothelial cell exocytosis which limits leukocyte recruitment. These data suggest that high density lipoprotein may inhibit diseases associated with vascular inflammation in part by blocking endothelial exocytosis.
authors.affiliation: Departments of Medicine, Division of Cardiology, University of Rochester School of Medicine, Box 679, 601 Elmwood Avenue, Rochester, NY, 14652, United States of America.|Aab Cardiovascular Research Institute, University of Rochester School of Medicine, Box CVRI, 601 Elmwood Avenue, Rochester, NY, 14652, United States of America.
authors.forename: Scott J
authors.initials: SJ
authors.lastname: Cameron
authors.affiliation: Aab Cardiovascular Research Institute, University of Rochester School of Medicine, Box CVRI, 601 Elmwood Avenue, Rochester, NY, 14652, United States of America.|Department of Comparative Medicine, The Johns Hopkins University School of Medicine 733 N. Broadway, MRB 827, Baltimore, MD, 21205, United States of America.
authors.forename: Craig N
authors.initials: CN
authors.lastname: Morrell
authors.affiliation: Department of Medicine, The Johns Hopkins University School of Medicine, 950 Ross Building, 720 Rutland Ave, Baltimore, MD, 21205, United States of America.
authors.forename: Clare
authors.initials: C
authors.lastname: Bao
authors.affiliation: Department of Comparative Medicine, The Johns Hopkins University School of Medicine 733 N. Broadway, MRB 827, Baltimore, MD, 21205, United States of America.
authors.forename: AnneMarie F
authors.initials: AF
authors.lastname: Swaim
authors.affiliation: Department of Cell Biology, University of Connecticut School of Medicine, E5050, 263 Farmington Avenue, Farmington, CT, 06030, United States of America.
authors.forename: Annabelle
authors.initials: A
authors.lastname: Rodriguez
authors.affiliation: Departments of Medicine, Division of Cardiology, University of Rochester School of Medicine, Box 679, 601 Elmwood Avenue, Rochester, NY, 14652, United States of America.|Aab Cardiovascular Research Institute, University of Rochester School of Medicine, Box CVRI, 601 Elmwood Avenue, Rochester, NY, 14652, United States of America.
authors.forename: Charles J
authors.initials: CJ
authors.lastname: Lowenstein
mesh_terms: D016632:Apolipoprotein A-I / Q000502:physiology; D002448:Cell Adhesion / Q000502:physiology; D002478:Cells, Cultured; D005089:Exocytosis; D006801:Humans; D007249:Inflammation / Q000517:prevention & control*; D008075:Lipoproteins, HDL / Q000502:physiology*; D020302:Weibel-Palade Bodies
chemical_list: D016632:Apolipoprotein A-I; D008075:Lipoproteins, HDL