title: Submolecular recognition of the C-terminal domain of the heavy chain of botulinum neurotoxin type A by T cells from toxin-treated cervical dystonia patients.
abstract: We determined the T-cell proliferative responses of the peripheral blood lymphocytes (PBL) from 25 botulinum neurotoxin (BoNT)-treated patients to 31 overlapping synthetic peptides encompassing the C-terminal half (residues 855-1296) of BoNT/A heavy chain. Responses of PBL to HC peptides varied among patients. Samples from 14 patients treated solely with BoNT/A recognized 2-13 (average 6.4) peptides/sample at Z>3.0 level. Six peptide regions representing residues 855-873, 1023-1041, 1051-1069, 1093-1111, 1135-1153 and 1247-1265 were frequently recognized by 36-57% of these PBLs. Influence of treatment parameters on T-cell recognition of the peptides was also investigated.
authors.affiliation: Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA.
authors.forename: Minako
authors.initials: M
authors.lastname: Oshima
authors.affiliation: Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA.
authors.forename: Philip
authors.initials: P
authors.lastname: Deitiker
authors.affiliation: Department of Neurology, Baylor College of Medicine, Houston, TX 77030, USA.
authors.forename: Joseph
authors.initials: J
authors.lastname: Jankovic
authors.affiliation: Allergan Inc., Irvine, CA 92612, USA.
authors.forename: K Roger
authors.initials: KR
authors.lastname: Aoki
authors.affiliation: Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA; Department of Pathology and Immunology, Baylor College of Medicine, Houston, TX 77030, USA. Electronic address: matassi@bcm.edu.
authors.forename: M Zouhair
authors.initials: MZ
authors.lastname: Atassi
mesh_terms: D000328:Adult; D000368:Aged; D000595:Amino Acid Sequence; D019274:Botulinum Toxins, Type A / Q000737:chemistry* / Q000276:immunology / Q000627:therapeutic use; D003014:Clostridium botulinum / Q000737:chemistry* / Q000276:immunology; D018604:Epitope Mapping; D018984:Epitopes, T-Lymphocyte / Q000737:chemistry* / Q000276:immunology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry* / Q000276:immunology; D061251:Primary Cell Culture; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D014103:Torticollis / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology
chemical_list: D018984:Epitopes, T-Lymphocyte; D010455:Peptides; D019274:Botulinum Toxins, Type A
keywords: Botulinum neurotoxin type A; Cervical dystonia; H(C) domain; MHC control; T-cell epitopes