title: A flavone-based turn-on fluorescent probe for intracellular cysteine/homocysteine sensing with high selectivity.
abstract: A new type of flavone-based fluorescent probe (DMAF) capable of cysteine (Cys)/homocysteine (Hcy) sensing with high selectivity over other amino acids was developed. Such type of probe undergoes Cys/Hcy-mediated cyclization reaction with the involvement of its aldehyde group, which suppresses of the photoinduced electron transfer (PET) process of the probe molecule and consequently leads to the enhancement of fluorescence emission upon excitation using visible light. The formation of product of the Cys/Hcy-mediated cyclization reaction was confirmed and the preliminary fluorescence imaging experiments revealed the biocompatibility of the as-prepared probe and validated its practicability for intracellular Cys/Hcy sensing.
authors.affiliation: School of Chemistry and Chemical Engineering, University of Chinese Academy of Sciences (UCAS), Beijing 100049, PR China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.affiliation: School of Chemistry and Chemical Engineering, University of Chinese Academy of Sciences (UCAS), Beijing 100049, PR China.
authors.forename: Yanlin
authors.initials: Y
authors.lastname: Lv
authors.affiliation: Beijing National Laboratory for Molecular Sciences (BNLMS), Key Laboratory of Photochemistry, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: School of Chemistry and Chemical Engineering, University of Chinese Academy of Sciences (UCAS), Beijing 100049, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Ding
authors.affiliation: Key Laboratory of Green Process and Engineering, Beijing Key Laboratory of Ionic Liquids Clean Process, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China.
authors.forename: Rongji
authors.initials: R
authors.lastname: Liu
authors.affiliation: Beijing National Laboratory for Molecular Sciences (BNLMS), Key Laboratory of Photochemistry, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, PR China. Electronic address: yszhao@iccas.ac.cn.
authors.forename: Yongsheng
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Green Process and Engineering, Beijing Key Laboratory of Ionic Liquids Clean Process, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China. Electronic address: zhanggj@home.ipe.ac.cn.
authors.forename: Guangjin
authors.initials: G
authors.lastname: Zhang
authors.affiliation: School of Chemistry and Chemical Engineering, University of Chinese Academy of Sciences (UCAS), Beijing 100049, PR China. Electronic address: zytian@ucas.ac.cn.
authors.forename: Zhiyuan
authors.initials: Z
authors.lastname: Tian
mesh_terms: D002623:Chemistry Techniques, Analytical / Q000295:instrumentation*; D003500:Cyclization; D003545:Cysteine / Q000032:analysis* / Q000737:chemistry; D004579:Electron Transport; D047309:Flavones / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006710:Homocysteine / Q000032:analysis*; D006801:Humans; D042541:Intracellular Space / Q000737:chemistry*; D008027:Light; D061986:MCF-7 Cells; D061848:Optical Imaging; D013050:Spectrometry, Fluorescence
chemical_list: D047309:Flavones; D005456:Fluorescent Dyes; D006710:Homocysteine; D003545:Cysteine; C043562:flavone
keywords: Bioimaging; Cysteine/homocysteine; Fluorescent probe