title: Decreased miR-134 expression and its tumor-suppressive function in human osteosarcoma.
abstract: Dysregulation of microRNA (miR) is often associated with cancer development and progression. Aberrant expression of miR-134 has been found in some types of cancer. However, its expression and function in osteosarcoma remain unclear. The aim of this study was to explore the effects of miR-134 in osteosarcoma tumorigenesis and development. The expression level of miR-134 was quantified by real-time reverse transcription-polymerase chain reaction in human osteosarcoma cell lines and tissues. The effects of miR-134 on MG-63 cell phenotypes and tumorigenicity in vivo were observed using flow cytometry, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, transwell invasion, migration, and scratch migration assays. MiR-134 was significantly downregulated in osteosarcoma cell lines and clinical specimens. Decreased miR-134 expression was significantly associated with large tumor size, positive distant metastasis, and advanced clinical stage. Low miR-134 expression in osteosarcoma was an independent predictor of poor survival. Overexpression of miR-134 inhibited MG-63 cell proliferation, invasion, and migration, promoted cell apoptosis in vitro, and suppressed tumorigenicity in vivo. These findings indicate that miR-134 may act as a tumor suppressor in osteosarcoma and could serve as a novel therapeutic agent for miRNA-based therapy.
authors.affiliation: Department of Orthopedics, Medical School of Yangtze University, Jingzhou, Hubei Province, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Bao
authors.affiliation: Department of Orthopedics, The Affiliated Hospital of Yangtze University, Jingzhou, Hubei Province, China.
authors.forename: L
authors.initials: L
authors.lastname: Peng
authors.affiliation: Department of Orthopedics, The Affiliated Hospital of Yangtze University, Jingzhou, Hubei Province, China.
authors.forename: J
authors.initials: J
authors.lastname: Ma
authors.affiliation: Department of Orthopedics, The Affiliated Hospital of Yangtze University, Jingzhou, Hubei Province, China.
authors.forename: K
authors.initials: K
authors.lastname: Liu
authors.affiliation: Department of Orthopedics, The Affiliated Hospital of Yangtze University, Jingzhou, Hubei Province, China.
authors.forename: W
authors.initials: W
authors.lastname: Li
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D015415:Biomarkers; D001859:Bone Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D016147:Genes, Tumor Suppressor; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging; D012516:Osteosarcoma / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D011379:Prognosis; D023041:Xenograft Model Antitumor Assays; D055815:Young Adult
chemical_list: D015415:Biomarkers; C569273:MIRN134 microRNA, human; D035683:MicroRNAs