title: Identification of N(α)-acetyl-α-lysine as a probable thermolyte and its accumulation mechanism in Salinicoccus halodurans H3B36.
abstract: Salinicoccus halodurans H3B36 is a moderate halophile that was isolated from a 3.2-m-deep sediment sample in Qaidam Basin, China. Our results suggest that N(α)-acetyl-α-lysine can accumulate and act as a probable thermolyte in this strain. The accumulation mechanism and biosynthetic pathway for this rare compatible solute were also elucidated. We confirmed that the de novo synthesis pathway of N(α)-acetyl-α-lysine in this strain starts from aspartate and passes through lysine. Through RNA sequencing, we also found an 8-gene cluster (orf_1582-1589) and another gene (orf_2472) that might encode the biosynthesis of N(α)-acetyl-α-lysine in S. halodurans H3B36. Orf_192, orf_193, and orf_1259 might participate in the transportation of precursors for generating N(α)-acetyl-α-lysine under the heat stress. The transcriptome reported here also generated a global view of heat-induced changes and yielded clues for studying the regulation of N(α)-acetyl-α-lysine accumulation. Heat stress triggered a global transcriptional disturbance and generated a series of actions to adapt the strain to heat stress. Furthermore, the transcriptomic results showed that the regulon of RpoN (orf_2534) may be critical to conferring heat stress tolerance and survival to S. halodurans.
authors.affiliation: State Key Laboratory of Microbial Resources and National Engineering Laboratory for Industrial Enzymes, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.|University of Chinese Academy of Sciences, Beijing, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Jiang
authors.affiliation: State Key Laboratory of Microbial Resources and National Engineering Laboratory for Industrial Enzymes, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Yanfen
authors.initials: Y
authors.lastname: Xue
authors.affiliation: State Key Laboratory of Microbial Resources and National Engineering Laboratory for Industrial Enzymes, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Yanhe
authors.initials: Y
authors.lastname: Ma
mesh_terms: D001224:Aspartic Acid / Q000378:metabolism; D002681:China; D019015:Geologic Sediments / Q000382:microbiology; D016931:Halobacteriales / Q000235:genetics / Q000378:metabolism; D018869:Heat-Shock Response / Q000235:genetics; D008239:Lysine / Q000096:biosynthesis / Q000378:metabolism*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012965:Sodium Chloride; D040141:Staphylococcaceae / Q000235:genetics / Q000378:metabolism*; D059467:Transcriptome / Q000235:genetics*
chemical_list: D012336:RNA, Ribosomal, 16S; D001224:Aspartic Acid; D012965:Sodium Chloride; D008239:Lysine