title: Injectable hydrogel systems crosslinked by horseradish peroxidase.
abstract: Hydrogels are widely used as reservoirs in drug delivery and scaffolds for tissue engineering. In particular, injectable hydrogel systems, which are formed by physical, chemical, or enzyme-mediated crosslinking reactions in situ, offer the advantages of minimal invasiveness, ease of application, and void-filling property. Examples of these hydrogels are provided in the first part of this paper. In the second part, hydrogels that are formed by the enzymatic activity of horseradish peroxidase (HRP) are highlighted. HRP catalyzes the crosslinking reaction of polymer-phenol conjugates in the presence of hydrogen peroxide (H2O2), resulting in hydrogels with tunable gelation rate and crosslinking density. The catalytic mechanism of the HRP-mediated crosslinking reaction is discussed in detail, and the recent biomedical applications of the HRP-crosslinked hydrogels are described. Lastly, the concerns associated with HRP-mediated crosslinking and the future outlook of HRP-crosslinked hydrogels are addressed.
authors.affiliation: Institute of Bioengineering and Nanotechnology, 31 Biopolis Way, The Nanos, #04-01, 138669 Singapore.
authors.forename: Fan
authors.initials: F
authors.lastname: Lee
authors.forename: Ki Hyun
authors.initials: KH
authors.lastname: Bae
authors.forename: Motoichi
authors.initials: M
authors.lastname: Kurisawa
mesh_terms: D001672:Biocompatible Materials / Q000008:administration & dosage / Q000138:chemical synthesis*; D003432:Cross-Linking Reagents / Q000737:chemistry*; D004789:Enzyme Activation; D004800:Enzymes, Immobilized / Q000737:chemistry; D006735:Horseradish Peroxidase / Q000737:chemistry*; D020100:Hydrogels / Q000008:administration & dosage / Q000737:chemistry*; D007267:Injections / Q000379:methods; D033581:Stem Cell Transplantation / Q000379:methods*; D023822:Tissue Engineering / Q000379:methods*; D014783:Viscosity
chemical_list: D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D004800:Enzymes, Immobilized; D020100:Hydrogels; D006735:Horseradish Peroxidase