title: Stable Force Balance between Epithelial Cells Arises from F-Actin Turnover.
abstract: The propagation of force in epithelial tissues requires that the contractile cytoskeletal machinery be stably connected between cells through E-cadherin-containing adherens junctions. In many epithelial tissues, the cells' contractile network is positioned at a distance from the junction. However, the mechanism or mechanisms that connect the contractile networks to the adherens junctions, and thus mechanically connect neighboring cells, are poorly understood. Here, we identified the role for F-actin turnover in regulating the contractile cytoskeletal network's attachment to adherens junctions. Perturbing F-actin turnover via gene depletion or acute drug treatments that slow F-actin turnover destabilized the attachment between the contractile actomyosin network and adherens junctions. Our work identifies a critical role for F-actin turnover in connecting actomyosin to intercellular junctions, defining a dynamic process required for the stability of force balance across intercellular contacts in tissues.
authors.affiliation: Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02142, USA.
authors.forename: Jeanne N
authors.initials: JN
authors.lastname: Jodoin
authors.affiliation: Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02142, USA.
authors.forename: Jonathan S
authors.initials: JS
authors.lastname: Coravos
authors.affiliation: Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02142, USA.
authors.forename: Soline
authors.initials: S
authors.lastname: Chanet
authors.affiliation: Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02142, USA.
authors.forename: Claudia G
authors.initials: CG
authors.lastname: Vasquez
authors.affiliation: Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02142, USA.
authors.forename: Michael
authors.initials: M
authors.lastname: Tworoger
authors.affiliation: Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02142, USA.
authors.forename: Elena R
authors.initials: ER
authors.lastname: Kingston
authors.affiliation: Department of Genetics, Harvard Medical School, Boston, MA 02115, USA.
authors.forename: Lizabeth A
authors.initials: LA
authors.lastname: Perkins
authors.affiliation: Department of Genetics, Harvard Medical School, Boston, MA 02115, USA; Howard Hughes Medical Institute, Boston, MA 02115, USA.
authors.forename: Norbert
authors.initials: N
authors.lastname: Perrimon
authors.affiliation: Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02142, USA. Electronic address: acmartin@mit.edu.
authors.forename: Adam C
authors.initials: AC
authors.lastname: Martin
mesh_terms: D000199:Actins / Q000378:metabolism*; D022005:Adherens Junctions / Q000378:metabolism*; D000818:Animals; D015820:Cadherins / Q000235:genetics; D003599:Cytoskeleton / Q000378:metabolism*; D004330:Drosophila; D004847:Epithelial Cells / Q000378:metabolism*; D004848:Epithelium / Q000378:metabolism*; D007365:Intercellular Junctions / Q000378:metabolism
chemical_list: D000199:Actins; D015820:Cadherins