title: Secretome analysis of chickpea reveals dynamic extracellular remodeling and identifies a Bet v1-like protein, CaRRP1 that participates in stress response.
abstract: Secreted proteins maintain cell structure and biogenesis besides acting in signaling events crucial for cellular homeostasis during stress adaptation. To understand the underlying mechanism of stress-responsive secretion, the dehydration-responsive secretome was developed from suspension-cultured cells of chickpea. Cell viability of the suspension culture remained unaltered until 96 h, which gradually declined at later stages of dehydration. Proteomic analysis led to the identification of 215 differentially regulated proteins, involved in a variety of cellular functions that include metabolism, cell defence, and signal transduction suggesting their concerted role in stress adaptation. One-third of the secreted proteins were devoid of N-terminal secretion signals suggesting a non-classical secretory route. Screening of the secretome identified a leaderless Bet v 1-like protein, designated CaRRP1, the export of which was inhibited by brefeldin A. We investigated the gene structure and genomic organization and demonstrated that CaRRP1 may be involved in stress response. Its expression was positively associated with abiotic and biotic stresses. CaRRP1 could complement the aberrant growth phenotype of yeast mutant, deficient in vesicular transport, indicating a partial overlap of protein secretion and stress response. Our study provides the most comprehensive analysis of dehydration-responsive secretome and the complex metabolic network operating in plant extracellular space.
authors.affiliation: National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi-110067, India.
authors.forename: Sonika
authors.initials: S
authors.lastname: Gupta
authors.affiliation: National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi-110067, India.
authors.forename: Vijay
authors.initials: V
authors.lastname: Wardhan
authors.affiliation: National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi-110067, India.
authors.forename: Amit
authors.initials: A
authors.lastname: Kumar
authors.affiliation: National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi-110067, India.
authors.forename: Divya
authors.initials: D
authors.lastname: Rathi
authors.affiliation: National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi-110067, India.
authors.forename: Aarti
authors.initials: A
authors.lastname: Pandey
authors.affiliation: National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi-110067, India.
authors.forename: Subhra
authors.initials: S
authors.lastname: Chakraborty
authors.affiliation: National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi-110067, India.
authors.forename: Niranjan
authors.initials: N
authors.lastname: Chakraborty
mesh_terms: D020413:3' Untranslated Regions; D000595:Amino Acid Sequence; D001483:Base Sequence; D020126:Brefeldin A / Q000494:pharmacology; D002374:Catalase / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D029863:Cicer / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D059828:Plant Cells / Q000145:classification / Q000378:metabolism; D018515:Plant Leaves / Q000378:metabolism; D010940:Plant Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D020543:Proteome / Q000032:analysis*; D040901:Proteomics; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D013312:Stress, Physiological; D053719:Tandem Mass Spectrometry; D014026:Nicotiana / Q000378:metabolism
chemical_list: D020413:3' Untranslated Regions; D010940:Plant Proteins; D020543:Proteome; D020126:Brefeldin A; D002374:Catalase