title: Differential expression of sema3A and sema7A in a murine model of multiple sclerosis: Implications for a therapeutic design.
abstract: We characterised the expression of semaphorin (sema)3A, sema7A and their receptors in the immune and the central nervous system (CNS) at different stages of experimental autoimmune encephalomyelitis (EAE). We also studied their expression in neonatal and adult oligodendrocyte progenitor cell (OPC) and in mature oligodendrocyte cultures. Our results show that sema3A is increased in the CNS and decreased in the immune system upon EAE induction. However, sema7A expression is increased in both the CNS and the immune system during EAE. We also detected sema3A, sema7A and their receptors in neonatal and adult OPCs and in mature oligodendrocytes. These data suggest that sema3A and sema7A are involved in the pathogenesis of EAE, in the modulation of the immune response and in the neurodegeneration that take place in the CNS. Sema7A may represent an intriguing potential therapeutic target for the treatment of both the neurodegenerative and immune-mediated disease processes in MS.
authors.affiliation: Servei de Neurologia-Neuroimmunologia, Centre d'Esclerosi Múltiple de Catalunya, Vall d'Hebron Institut de Recerca, Hospital UniversitariVall d'Hebron, 08035 Barcelona, Spain; Universitat Autònoma de Barcelona, 08193, Bellaterra, Cerdanyola del Vallès, Spain. Electronic address: agutierrez@igtp.cat.
authors.forename: Ana
authors.initials: A
authors.lastname: Gutiérrez-Franco
authors.affiliation: Servei de Neurologia-Neuroimmunologia, Centre d'Esclerosi Múltiple de Catalunya, Vall d'Hebron Institut de Recerca, Hospital UniversitariVall d'Hebron, 08035 Barcelona, Spain; Universitat Autònoma de Barcelona, 08193, Bellaterra, Cerdanyola del Vallès, Spain. Electronic address: carme.costa@vhir.org.
authors.forename: Carme
authors.initials: C
authors.lastname: Costa
authors.affiliation: Servei de Neurologia-Neuroimmunologia, Centre d'Esclerosi Múltiple de Catalunya, Vall d'Hebron Institut de Recerca, Hospital UniversitariVall d'Hebron, 08035 Barcelona, Spain; Universitat Autònoma de Barcelona, 08193, Bellaterra, Cerdanyola del Vallès, Spain. Electronic address: herena.eixarch@vhir.org.
authors.forename: Herena
authors.initials: H
authors.lastname: Eixarch
authors.affiliation: Servei de Neurologia-Neuroimmunologia, Centre d'Esclerosi Múltiple de Catalunya, Vall d'Hebron Institut de Recerca, Hospital UniversitariVall d'Hebron, 08035 Barcelona, Spain; Universitat Autònoma de Barcelona, 08193, Bellaterra, Cerdanyola del Vallès, Spain. Electronic address: mireia.castillo@vhir.org.
authors.forename: Mireia
authors.initials: M
authors.lastname: Castillo
authors.affiliation: Grupo de Neurobiología del Desarrollo, Hospital Nacional de Parapléjicos, 45071 Toledo, Spain. Electronic address: emedinar@sescam.jccm.es.
authors.forename: Eva M
authors.initials: EM
authors.lastname: Medina-Rodríguez
authors.affiliation: Grupo de Neurobiología del Desarrollo, Hospital Nacional de Parapléjicos, 45071 Toledo, Spain; Instituto Cajal-CSIC, 28002-Madrid, Spain. Electronic address: abribian@cajal.csic.es.
authors.forename: Ana
authors.initials: A
authors.lastname: Bribián
authors.affiliation: Grupo de Neurobiología del Desarrollo, Hospital Nacional de Parapléjicos, 45071 Toledo, Spain; Instituto Cajal-CSIC, 28002-Madrid, Spain. Electronic address: fdecastro@cajal.csic.es.
authors.forename: Fernando
authors.initials: F
authors.lastname: de Castro
authors.affiliation: Servei de Neurologia-Neuroimmunologia, Centre d'Esclerosi Múltiple de Catalunya, Vall d'Hebron Institut de Recerca, Hospital UniversitariVall d'Hebron, 08035 Barcelona, Spain; Universitat Autònoma de Barcelona, 08193, Bellaterra, Cerdanyola del Vallès, Spain. Electronic address: xavier.montalban@cem-cat.org.
authors.forename: Xavier
authors.initials: X
authors.lastname: Montalban
authors.affiliation: Servei de Neurologia-Neuroimmunologia, Centre d'Esclerosi Múltiple de Catalunya, Vall d'Hebron Institut de Recerca, Hospital UniversitariVall d'Hebron, 08035 Barcelona, Spain; Universitat Autònoma de Barcelona, 08193, Bellaterra, Cerdanyola del Vallès, Spain. Electronic address: carmen.espejo@vhir.org.
authors.forename: Carmen
authors.initials: C
authors.lastname: Espejo
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000235:genetics / Q000276:immunology*; D001921:Brain / Q000276:immunology / Q000473:pathology; D004195:Disease Models, Animal; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000235:genetics / Q000276:immunology*; D005434:Flow Cytometry; D005786:Gene Expression Regulation; D015151:Immunoblotting; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D009103:Multiple Sclerosis / Q000276:immunology; D009836:Oligodendroglia / Q000276:immunology; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D040121:Semaphorin-3A / Q000235:genetics / Q000276:immunology*; D039961:Semaphorins / Q000235:genetics / Q000276:immunology*; D013116:Spinal Cord / Q000276:immunology / Q000473:pathology
chemical_list: D015703:Antigens, CD; C488217:Sema3a protein, mouse; C485571:Sema7a protein, mouse; D040121:Semaphorin-3A; D039961:Semaphorins
keywords: Experimental autoimmune encephalomyelitis; Immune response; Multiple sclerosis; Neurodegeneration; Semaphorin