title: Systems Proteomics View of the Endogenous Human Claudin Protein Family.
abstract: Claudins are the major transmembrane protein components of tight junctions in human endothelia and epithelia. Tissue-specific expression of claudin members suggests that this protein family is not only essential for sustaining the role of tight junctions in cell permeability control but also vital in organizing cell contact signaling by protein-protein interactions. How this protein family is collectively processed and regulated is key to understanding the role of junctional proteins in preserving cell identity and tissue integrity. The focus of this review is to first provide a brief overview of the functional context, on the basis of the extensive body of claudin biology research that has been thoroughly reviewed, for endogenous human claudin members and then ascertain existing and future proteomics techniques that may be applicable to systematically characterizing the chemical forms and interacting protein partners of this protein family in human. The ability to elucidate claudin-based signaling networks may provide new insight into cell development and differentiation programs that are crucial to tissue stability and manipulation.
authors.forename: Fei
authors.initials: F
authors.lastname: Liu
authors.affiliation: Department of Medicine, Division of Pulmonary, Allergy, Critical Care and Sleep Medicine, and Department of Cell Biology, Emory University School of Medicine , 205 Whitehead Biomedical Research Building, 615 Michael Street, Atlanta, Georgia 30322, United States.
authors.forename: Michael
authors.initials: M
authors.lastname: Koval
authors.forename: Shoba
authors.initials: S
authors.lastname: Ranganathan
authors.forename: Susan
authors.initials: S
authors.lastname: Fanayan
authors.affiliation: Barnett Institute and Department of Chemistry and Chemical Biology, Northeastern University , Boston, Massachusetts 02115, United States.
authors.forename: William S
authors.initials: WS
authors.lastname: Hancock
authors.affiliation: SciLifeLab, School of Biotechnology, Royal Institute of Technology (KTH) , SE-171 21 Solna, Stockholm, Sweden.
authors.forename: Emma K
authors.initials: EK
authors.lastname: Lundberg
authors.affiliation: Department of Biochemistry and Medical Genetics, University of Manitoba , 744 Bannatyne Avenue, Winnipeg, Manitoba R3E 0W3, Canada.
authors.forename: Ronald C
authors.initials: RC
authors.lastname: Beavis
authors.affiliation: SIB-Swiss Institute of Bioinformatics , CMU - Rue Michel-Servet 1, 1211 Geneva, Switzerland.
authors.forename: Lydie
authors.initials: L
authors.lastname: Lane
authors.affiliation: SIB-Swiss Institute of Bioinformatics , CMU - Rue Michel-Servet 1, 1211 Geneva, Switzerland.
authors.forename: Paula
authors.initials: P
authors.lastname: Duek
authors.forename: Leon
authors.initials: L
authors.lastname: McQuade
authors.affiliation: Department of Chemistry, Department of Molecular Biosciences, and Proteomics Center of Excellence, Northwestern University , 2145 North Sheridan Road, Evanston, Illinois 60208, United States.
authors.forename: Neil L
authors.initials: NL
authors.lastname: Kelleher
authors.forename: Mark S
authors.initials: MS
authors.lastname: Baker
mesh_terms: D057167:Claudins / Q000235:genetics / Q000378:metabolism*; D004727:Endothelium / Q000378:metabolism; D004848:Epithelium / Q000378:metabolism; D006031:Glycosylation; D006801:Humans; D005810:Multigene Family; D060066:Protein Interaction Maps; D040901:Proteomics / Q000379:methods*; D015398:Signal Transduction; D019108:Tight Junctions / Q000378:metabolism*
chemical_list: D057167:Claudins
keywords: Membrane protein complexes; cell-contact signaling; chemical proteomics; membrane proteomics; systems proteomics; targeted proteomics; top-down proteomics