title: Production of Brazilian human norovirus VLPs and comparison of purification methods.
abstract: Noroviruses (NVs) are responsible for most cases of human nonbacterial gastroenteritis worldwide. Some parameters for the purification of NV virus-like particles (VLPs) such as ease of production and yield were studied for future development of vaccines and diagnostic tools. In this study, VLPs were produced by the expression of the VP1 and VP2 gene cassette of the Brazilian NV isolate, and two purification methods were compared: cesium chloride (CsCl) gradient centrifugation and ion-exchange chromatography (IEC). IEC produced more and purer VLPs of NV compared to CsCl gradient centrifugation.
authors.affiliation: Departamento de Farmácia, Universidade de Brasília, Brasília, DF, Brazil.
authors.forename: Thais Alves da Costa
authors.initials: TA
authors.lastname: Lamounier
authors.affiliation: Departamento de Biologia Celular, Universidade de Brasília, Brasília, DF, Brazil.
authors.forename: Layssa Miranda
authors.initials: LM
authors.lastname: de Oliveira
authors.affiliation: Departamento de Biologia Celular, Universidade de Brasília, Brasília, DF, Brazil.
authors.forename: Brenda Rabello
authors.initials: BR
authors.lastname: de Camargo
authors.affiliation: Departamento de Biologia Celular, Universidade de Brasília, Brasília, DF, Brazil.
authors.forename: Kelly Barreto
authors.initials: KB
authors.lastname: Rodrigues
authors.affiliation: Departamento de Biologia Celular, Universidade de Brasília, Brasília, DF, Brazil.
authors.forename: Eliane Ferreira
authors.initials: EF
authors.lastname: Noronha
authors.affiliation: Departamento de Biologia Celular, Universidade de Brasília, Brasília, DF, Brazil.
authors.forename: Bergmann Morais
authors.initials: BM
authors.lastname: Ribeiro
authors.affiliation: Departamento de Biologia Celular, Universidade de Brasília, Brasília, DF, Brazil.
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Nagata
mesh_terms: D001938:Brazil; D002499:Centrifugation, Density Gradient / Q000379:methods*; D002648:Child; D002852:Chromatography, Ion Exchange / Q000379:methods*; D006801:Humans; D029322:Norovirus / Q000235:genetics*; D015678:Viral Structural Proteins / Q000235:genetics* / Q000378:metabolism; D022701:Virosomes / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D015678:Viral Structural Proteins; D022701:Virosomes