title: Mitochondria-Targeted Fluorescent Probe for Imaging Hydrogen Peroxide in Living Cells.
abstract: Hydrogen peroxide (H2O2), as a type of reactive oxygen species (ROS), can be endogenously produced from the mitochondrial electron transport chain in aerobic respiration and plays important roles in several physiological processes. However, the design and synthesis of fluorescent probes, which can detect mitochondrial H2O2 in living cells, still remain rare. Herein, we report the preparation of a novel cationic probe 1 (Mito-H2O2), which targets the mitochondria in living cells and is sensitive to the presence of H2O2. The probe Mito-H2O2 displays desired properties such as high specificity, "Turn-On" fluorescence response with suitable sensitivity, appreciable water solubility, and rapid response time (within 5 min). The sensing mechanism was confirmed by high-resolution mass spectroscopy analysis, and the mechanism of "Turn-On" fluorescent response was also determined using a density functional theory (DFT) calculation method. Moreover, as a biocompatible molecule, the probe Mito-H2O2 has been successfully applied for the detection of the intrinsically generated intracellular H2O2 in living cells, and the fluorescence colocalization studies indicate that the probe localizes solely in the mitochondria of HeLa cells.
authors.affiliation: Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences , Lanzhou, Gansu 730000, P. R. China.
authors.forename: Jian
authors.initials: J
authors.lastname: Xu
authors.affiliation: College of Physics and Electronic Engineering, Northwest Normal University , Lanzhou, Gansu 730070, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences , Lanzhou, Gansu 730000, P. R. China.
authors.forename: Hui
authors.initials: H
authors.lastname: Yu
authors.affiliation: Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences , Lanzhou, Gansu 730000, P. R. China.
authors.forename: Xudong
authors.initials: X
authors.lastname: Gao
authors.affiliation: Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences , Lanzhou, Gansu 730000, P. R. China.
authors.forename: Shijun
authors.initials: S
authors.lastname: Shao
mesh_terms: D002470:Cell Survival; D005456:Fluorescent Dyes / Q000032:analysis / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D006861:Hydrogen Peroxide / Q000032:analysis*; D008928:Mitochondria / Q000737:chemistry*; D061848:Optical Imaging; D011789:Quantum Theory
chemical_list: D005456:Fluorescent Dyes; D006861:Hydrogen Peroxide