title: Impact of Variations in Test Method Parameters on In Vitro Activity of Surotomycin against Clostridium difficile and Surotomycin Quality Control Limits for Broth Microdilution and Agar Dilution Susceptibility Testing.
abstract: Test parameter variations were evaluated for their effects on surotomycin MICs. Calcium concentration was the only variable that influenced MICs; therefore, 50 μg/ml (standard for lipopeptide testing) is recommended. Quality control ranges for Clostridium difficile (0.12 to 1 μg/ml) and Eggerthella lenta (broth, 1 to 4 μg/ml; agar, 1 to 8 μg/ml) were approved by the Clinical and Laboratory Standards Institute based on these data.
authors.affiliation: Clinical Microbiology Institute, Wilsonville, Oregon, USA.
authors.forename: Maria M
authors.initials: MM
authors.lastname: Traczewski
authors.affiliation: Eurofins Medinet, Chantilly, Virginia, USA.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Deane
authors.affiliation: Eurofins Medinet, Chantilly, Virginia, USA.
authors.forename: Daniel
authors.initials: D
authors.lastname: Sahm
authors.affiliation: Clinical Microbiology Institute, Wilsonville, Oregon, USA.
authors.forename: Steven D
authors.initials: SD
authors.lastname: Brown
authors.affiliation: Merck and Co., Inc., Kenilworth, New Jersey, USA laurent.chesnel@merck.com.
authors.forename: Laurent
authors.initials: L
authors.lastname: Chesnel
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D016360:Clostridioides difficile / Q000187:drug effects*; D006801:Humans; D055666:Lipopeptides / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests / Q000379:methods* / Q000592:standards*; D010456:Peptides, Cyclic / Q000494:pharmacology*; D011786:Quality Control; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000900:Anti-Bacterial Agents; C574454:CB-183,315; D055666:Lipopeptides; D010456:Peptides, Cyclic