title: Gene expression analysis upon lncRNA DDSR1 knockdown in human fibroblasts.
abstract: Long non-coding RNAs (lncRNAs) play important roles in regulating diverse biological processes including DNA damage and repair. We have recently reported that the DNA damage inducible lncRNA DNA damage-sensitive RNA1 (DDSR1) regulates DNA repair by homologous recombination (HR). Since lncRNAs also modulate gene expression, we identified gene expression changes upon DDSR1 knockdown in human fibroblast cells. Gene expression analysis after RNAi treatment targeted against DDSR1 revealed 119 genes that show differential expression. Here we provide a detailed description of the microarray data (NCBI GEO accession number GSE67048) and the data analysis procedure associated with the publication by Sharma et al., 2015 in EMBO Reports [1].
authors.affiliation: CCR Collaborative Bioinformatics Resource (CCBR), National Cancer Institute, NIH, Bethesda, MD 20892, USA.
authors.forename: Li
authors.initials: L
authors.lastname: Jia
authors.affiliation: Cancer Research Technology Program, Leidos Biomedical Research, Inc., Frederick National Laboratory for Cancer Research, Frederick, MD 21702, USA.
authors.forename: Zhonghe
authors.initials: Z
authors.lastname: Sun
authors.affiliation: Cancer Research Technology Program, Leidos Biomedical Research, Inc., Frederick National Laboratory for Cancer Research, Frederick, MD 21702, USA.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Wu
authors.affiliation: Cell Biology of Genomes Group, National Cancer Institute, NIH, Bethesda, MD 20892, USA.
authors.forename: Tom
authors.initials: T
authors.lastname: Misteli
authors.affiliation: Cell Biology of Genomes Group, National Cancer Institute, NIH, Bethesda, MD 20892, USA.
authors.forename: Vivek
authors.initials: V
authors.lastname: Sharma
keywords: DDSR1; lncRNA