title: Fast Centrifugal Partition Chromatography Fractionation of Concentrated Agave (Agave salmiana) Sap to Obtain Saponins with Apoptotic Effect on Colon Cancer Cells.
abstract: Separation of potentially bioactive components from foods and plant extracts is one of the main challenges for their study. Centrifugal partition chromatography has been a successful technique for the screening and identification of molecules with bioactive potential, such as steroidal saponins. Agave is a source of steroidal saponins with anticancer potential, though the activity of these compounds in concentrated agave sap has not been yet explored. In this study, fast centrifugal partition chromatography (FCPC) was used coupled with in vitro tests on HT-29 cells as a screening procedure to identify apoptotic saponins from an acetonic extract of concentrated agave sap. The three most bioactive fractions obtained by FCPC at partition coefficients between 0.23 and 0.4 contained steroidal saponins, predominantly magueyoside b. Flow cytometry analysis determined that the fraction rich in kammogenin and manogenin glycosides induced apoptosis, but when gentrogenin and hecogenin glycosides were also found in the fraction, a necrotic effect was observed. In conclusion, this study provides the evidence that steroidal saponins in concentrated agave sap were potential inductors of apoptosis and that it was possible to separate them using fast centrifugal partition chromatography.
authors.affiliation: Centro de Biotecnología-FEMSA, Escuela de Ingeniería y Ciencias, Tecnológico de Monterrey, Campus Monterrey, Av. Eugenio Garza Sada 2501 Sur, C.P. 64849, Monterrey, NL, Mexico.
authors.forename: Liliana
authors.initials: L
authors.lastname: Santos-Zea
authors.affiliation: Centro de Innovación y Transferencia en Salud, Tecnológico de Monterrey, Campus Monterrey, Av. Morones Prieto 3000 Pte., C.P. 64710, Monterrey, NL, Mexico.
authors.forename: Oscar R
authors.initials: OR
authors.lastname: Fajardo-Ramírez
authors.affiliation: Centro de Biotecnología-FEMSA, Escuela de Ingeniería y Ciencias, Tecnológico de Monterrey, Campus Monterrey, Av. Eugenio Garza Sada 2501 Sur, C.P. 64849, Monterrey, NL, Mexico.
authors.forename: Irasema
authors.initials: I
authors.lastname: Romo-López
authors.affiliation: Centro de Biotecnología-FEMSA, Escuela de Ingeniería y Ciencias, Tecnológico de Monterrey, Campus Monterrey, Av. Eugenio Garza Sada 2501 Sur, C.P. 64849, Monterrey, NL, Mexico. jagu@itesm.mx.
authors.forename: Janet A
authors.initials: JA
authors.lastname: Gutiérrez-Uribe
mesh_terms: D000096:Acetone; D027882:Agave / Q000737:chemistry*; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D002498:Centrifugation; D005591:Chemical Fractionation; D002845:Chromatography; D003110:Colonic Neoplasms / Q000188:drug therapy*; D019073:HT29 Cells; D006801:Humans; D013058:Mass Spectrometry; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D012502:Sapogenins / Q000032:analysis / Q000302:isolation & purification / Q000494:pharmacology; D012503:Saponins / Q000032:analysis / Q000302:isolation & purification* / Q000494:pharmacology
chemical_list: D000970:Antineoplastic Agents; D010936:Plant Extracts; D012502:Sapogenins; D012503:Saponins; D000096:Acetone; C007908:hecogenin
keywords: Agave sap; Anticancer; Fast centrifugal partition chromatography; Gentrogenin; Hecogenin; Kammogenin; Manogenin; Saponins