title: Treatment of endosulfan contaminated water with in vitro plant cell cultures.
abstract: Endosulfan is a Persistent Organic Pollutant insecticide still used in many countries. It is commercially available as mixtures of two diastereomers, α- and β-endosulfan, known as technical grade endosulfan (TGE). A laboratory model based on the use of axenic plant cell cultures to study the removal and metabolization of both isomers from contaminated water matrixes was established. No differences were recorded in the removal of the two individual isomers with the two tested endemic plants, Grindelia pulchella and Tessaria absinthioides. Undifferentiated cultures of both plant species were very efficient to lower endosulfan concentration in spiked solutions. Metabolic fate of TGE was evaluated by analyzing the time course of endosulfan metabolites accumulation in both plant biomass and bioremediation media. While in G. pulchella we only detected endosulfan sulfate, in T. absinthioides the non-toxic endosulfan alcohol was the main metabolite at 48h, giving the possibility of designing phytoremediation approaches.
authors.affiliation: INTEQUI-CONICET-Área de Química Orgánica, Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, Chacabuco y Pedernera, D5700HHW San Luis, Argentina; CEPROCOR, Complejo Hospitalario Santa María de Punilla, X5004AAP Córdoba, Argentina.
authors.forename: Patricia A
authors.initials: PA
authors.lastname: Lucero
authors.affiliation: INTEQUI-CONICET-Área de Química Orgánica, Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, Chacabuco y Pedernera, D5700HHW San Luis, Argentina.
authors.forename: Mónica M
authors.initials: MM
authors.lastname: Ferrari
authors.affiliation: INTEQUI-CONICET-Área de Química Orgánica, Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, Chacabuco y Pedernera, D5700HHW San Luis, Argentina.
authors.forename: Alejandro A
authors.initials: AA
authors.lastname: Orden
authors.affiliation: CEPROCOR, Complejo Hospitalario Santa María de Punilla, X5004AAP Córdoba, Argentina.
authors.forename: Irene
authors.initials: I
authors.lastname: Cañas
authors.affiliation: CEPROCOR, Complejo Hospitalario Santa María de Punilla, X5004AAP Córdoba, Argentina.
authors.forename: Mirtha
authors.initials: M
authors.lastname: Nassetta
authors.affiliation: INTEQUI-CONICET-Área de Química Orgánica, Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, Chacabuco y Pedernera, D5700HHW San Luis, Argentina. Electronic address: mkurina@unsl.edu.ar.
authors.forename: Marcela
authors.initials: M
authors.lastname: Kurina-Sanz
mesh_terms: D019659:Asteraceae / Q000378:metabolism*; D001673:Biodegradation, Environmental; D004726:Endosulfan / Q000378:metabolism*; D007306:Insecticides / Q000378:metabolism*; D059828:Plant Cells / Q000378:metabolism*; D013045:Species Specificity; D014874:Water Pollutants, Chemical / Q000378:metabolism*
chemical_list: D007306:Insecticides; D014874:Water Pollutants, Chemical; D004726:Endosulfan
keywords: Endosulfan; Grindelia pulchella; Phytometabolization; Phytoremediation; Tessaria absinthioides