title: Characterization of a novel 8R,11S-linoleate diol synthase from Penicillium chrysogenum by identification of its enzymatic products.
abstract: To identify novel fatty acid diol synthases, putative candidate sequences from Penicillium species were analyzed, and hydroxy fatty acid production by crude Penicillium enzyme extracts was assessed. Penicillium chrysogenum was found to produce an unknown dihydroxy fatty acid, a candidate gene implicated in this production was cloned and expressed, and the expressed enzyme was purified. The product obtained by the reaction of the purified enzyme with linoleic acid was identified as 8R,11S-dihydroxy-9,12(Z,Z)-octadecadienoic acid (8R,11S-DiHODE). The catalytic efficiency of this enzyme toward linoleic acid was the highest among the unsaturated fatty acids tested, indicating that this enzyme was a novel 8R,11S-linoleate diol synthase (8R,11S-LDS). A sexual stage in the life cycle of P. chrysogenum has recently been discovered, and 8R,11S-DiHODE produced by 8R,11S-LDS may constitute a precocious sexual inducer factor, responsible for regulating the sexual and asexual cycles of this fungus.
authors.affiliation: Department of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Kyung-Chul
authors.initials: KC
authors.lastname: Shin
authors.affiliation: Department of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Min-Ju
authors.initials: MJ
authors.lastname: Seo
authors.affiliation: Department of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, Republic of Korea deokkun@konkuk.ac.kr.
authors.forename: Deok-Kun
authors.initials: DK
authors.lastname: Oh
mesh_terms: D002384:Catalysis; D002853:Chromatography, Liquid; D008018:Life Cycle Stages / Q000235:genetics; D008041:Linoleic Acids / Q000737:chemistry* / Q000378:metabolism; D010105:Oxygenases / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D010408:Penicillium chrysogenum / Q000201:enzymology* / Q000502:physiology; D012100:Reproduction, Asexual / Q000235:genetics*; D013237:Stereoisomerism
chemical_list: D008041:Linoleic Acids; D010105:Oxygenases; C122014:linoleate diol synthase
keywords: 8R,11S-dihydroxy-9,12(Z,Z)-octadecadienoic acid; hydroxy fatty acid; precocious sexual inducer factor