title: Tyrosinase-Mediated Construction of a Silk Fibroin/Elastin Nanofiber Bioscaffold.
abstract: Elastin has characteristics of elasticity, biological activity, and mechanical stability. In the present work, tyrosinase-mediated construction of a bioscaffold with silk fibroin and elastin was carried out, aiming at developing a novel medical biomaterial. The efficiency of enzymatic oxidation of silk fibroin and the covalent reaction between fibroin and elastin were examined by spectrophotometry, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), and size exclusion chromatography (SEC). The properties of composite air-dried and nanofiber scaffolds were investigated. The results reveal that elastin was successfully bonded to silk fibroins, resulting in an increase in molecular weight of fibroin proteins. ATR-FTIR spectra indicated that tyrosinase treatment impacted the conformational structure of fibroin-based membrane. The thermal behaviors and mechanical properties of the tyrosinase-treated scaffolds were also improved compared with the untreated group. NIH/3T3 cells exhibited optimum densities when grown on the nanofiber scaffold, implying that the nanofiber scaffold has enhanced biocompatibility compared to the air-dried scaffold. A biological nanofiber scaffold constructed from tyrosinase-treated fibroin and elastin could potentially be utilized in biomedical applications.
authors.affiliation: Key Laboratory of Science and Technology of Eco-Textile, Ministry of Education, Jiangnan University, Wuxi, 214122, People's Republic of China.
authors.forename: Yanqing
authors.initials: Y
authors.lastname: Hong
authors.affiliation: Key Laboratory of Science and Technology of Eco-Textile, Ministry of Education, Jiangnan University, Wuxi, 214122, People's Republic of China.
authors.forename: Xueke
authors.initials: X
authors.lastname: Zhu
authors.affiliation: Key Laboratory of Science and Technology of Eco-Textile, Ministry of Education, Jiangnan University, Wuxi, 214122, People's Republic of China. pwang@jiangnan.edu.cn.
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.affiliation: School of Pharmaceutics Science, Jiangnan University, Wuxi, 214122, People's Republic of China.
authors.forename: Haitian
authors.initials: H
authors.lastname: Fu
authors.affiliation: Wuxi Medical School, Jiangnan University, Wuxi, 214122, People's Republic of China.
authors.forename: Chao
authors.initials: C
authors.lastname: Deng
authors.affiliation: Key Laboratory of Science and Technology of Eco-Textile, Ministry of Education, Jiangnan University, Wuxi, 214122, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Cui
authors.affiliation: Key Laboratory of Science and Technology of Eco-Textile, Ministry of Education, Jiangnan University, Wuxi, 214122, People's Republic of China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Key Laboratory of Science and Technology of Eco-Textile, Ministry of Education, Jiangnan University, Wuxi, 214122, People's Republic of China.
authors.forename: Xuerong
authors.initials: X
authors.lastname: Fan
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000627:therapeutic use; D004549:Elastin / Q000737:chemistry* / Q000378:metabolism; D005349:Fibroins / Q000737:chemistry* / Q000378:metabolism; D051379:Mice; D014442:Monophenol Monooxygenase / Q000737:chemistry* / Q000378:metabolism; D041681:NIH 3T3 Cells; D057139:Nanofibers / Q000737:chemistry; D047011:Silk / Q000737:chemistry; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials; D047011:Silk; D004549:Elastin; D005349:Fibroins; D014442:Monophenol Monooxygenase
keywords: Biocompatibility; Bioscaffold; Elastin; Silk fibroin; Tyrosinase