title: Endometrial Mesenchymal Stem Cells Isolated from Menstrual Blood by Adherence.
abstract: Objective. To find a convenient and efficient way to isolate MSCs from human menstrual blood and to investigate their biological characteristics, proliferative capacity, and secretion levels. Methods. MSCs were isolated from menstrual blood of 3 healthy women using adherence. Cell immunological phenotype was examined by flow cytometry; the adipogenic, osteogenic, and chondrogenic differentiation of MSCs was examined by Oil-Red-O staining, ALP staining, and Alcian Blue staining, respectively; and the secretion of cytokines, including vascular endothelial growth factor (VEGF), hepatocyte growth factor (HGF), and insulin-like growth factor-1 (IGF-1), was detected using enzyme-linked immunosorbent assay. Results. MB-MSCs were successfully isolated from human menstrual blood using adherence. They were positive for CD73, CD105, CD29, and CD44, but negative for CD31 and CD45. The differentiated MB-MSCs were positive for ALP staining, Oil-Red-O staining, and Alcian Blue staining. In addition, they could secrete antiapoptotic cytokines, such as VEGF, IGF-1, and HGF. Conclusion. It is feasible to isolate MSCs from human menstrual blood, thus avoiding invasive procedures and ethical controversies. Adherence could be a promising alternative to the density gradient centrifugation for the isolation of MSCs from menstrual blood.
authors.affiliation: Department of Obstetrics & Gynecology, General Hospital, Tianjin Medical University, Tianjin 300052, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Du
authors.affiliation: Department of Obstetrics & Gynecology, General Hospital, Tianjin Medical University, Tianjin 300052, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Yuan
authors.affiliation: Department of Pathogenic Biology and Immunology, Logistics College of Chinese People's Armed Police Forces, Tianjin 300309, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Qu
authors.affiliation: State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300020, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Department of Biomedical Engineering, University of Dundee, Dundee DD1 4HN, UK.
authors.forename: Jia
authors.initials: J
authors.lastname: Bei