title: The human carbonic anhydrase isoenzymes I and II inhibitory effects of some hydroperoxides, alcohols, and acetates.
abstract: The carbonic anhydrases (CAs, EC 4.2.1.1) represent a superfamily of widespread enzymes, which catalyze a crucial biochemical reaction, the reversible hydration of carbon dioxide to bicarbonate and protons. Human CA isoenzymes I and II (hCA I and hCA II) are ubiquitous cytosolic isoforms. In this study, a series of hydroperoxides, alcohols, and acetates were tested for the inhibition of the cytosolic hCA I and II isoenzymes. These compounds inhibited both hCA isozymes in the low nanomolar ranges. These compounds were good hCA I inhibitors (Kis in the range of 24.93-97.99 nM) and hCA II inhibitors (Kis in the range of 26.04-68.56 nM) compared to acetazolamide as CA inhibitor (Ki: 34.50 nM for hCA I and Ki: 28.93 nM for hCA II).
authors.affiliation: a Department of Chemistry, Faculty of Sciences , Atatürk University , Erzurum , Turkey .
authors.forename: Leyla Polat
authors.initials: LP
authors.lastname: Kose
authors.affiliation: a Department of Chemistry, Faculty of Sciences , Atatürk University , Erzurum , Turkey .|b Department of Zoology , College of Science, King Saud University , Saudi Arabia .
authors.forename: İlhami
authors.initials: İ
authors.lastname: Gulcin
authors.affiliation: a Department of Chemistry, Faculty of Sciences , Atatürk University , Erzurum , Turkey .
authors.forename: Alper
authors.initials: A
authors.lastname: Yıldırım
authors.affiliation: a Department of Chemistry, Faculty of Sciences , Atatürk University , Erzurum , Turkey .
authors.forename: Ufuk
authors.initials: U
authors.lastname: Atmaca
authors.affiliation: a Department of Chemistry, Faculty of Sciences , Atatürk University , Erzurum , Turkey .
authors.forename: Murat
authors.initials: M
authors.lastname: Çelik
authors.affiliation: b Department of Zoology , College of Science, King Saud University , Saudi Arabia .
authors.forename: Saleh H
authors.initials: SH
authors.lastname: Alwasel
authors.affiliation: c Dipartimento Di Chimica Ugo Schiff , Università Degli Studi Di Firenze , Firenze , Italy , and.|d Neurofarba Department , Section of Pharmaceutical and Nutriceutical Sciences, Università Degli Studi Di Firenze , Florence , Italy.
authors.forename: Claudiu T
authors.initials: CT
authors.lastname: Supuran
mesh_terms: D000085:Acetates / Q000494:pharmacology*; D000438:Alcohols / Q000494:pharmacology*; D024401:Carbonic Anhydrase I / Q000037:antagonists & inhibitors*; D024402:Carbonic Anhydrase II / Q000037:antagonists & inhibitors*; D002257:Carbonic Anhydrase Inhibitors / Q000494:pharmacology*; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*
chemical_list: D000085:Acetates; D000438:Alcohols; D002257:Carbonic Anhydrase Inhibitors; D006861:Hydrogen Peroxide; D024401:Carbonic Anhydrase I; D024402:Carbonic Anhydrase II
keywords: Acetate; alcohol; carbonic anhydrase; enzyme inhibition; enzyme purification; hydroperoxide