title: Efficacy of feed additives against Campylobacter in live broilers during the entire rearing period: Part B.
abstract: A total of 636 day-of-hatch Ross 308 broilers chicks were used in 4 independent trials carried out to screen the effect of 12 feed additives on reducing cecal colonization of Campylobacterin broilers. The tested additives were probiotics based on B. subtilis and S. cerevisae, a garlic extract, a blend of herbal substances and essential oils, two different combinations of essential oils and organic acids (OA), two mixtures of flavoring compounds, medium chain fatty acids (MCFA), monoglycerides (MG) of MCFA and MG-MCFA+OA. At 14 days of age, all the birds were orally infected with 0.1 mL of a bacterial suspension of C. jejuni ST-45 diluted at 10(5) cfu/mL in tryptone salt broth. In each trial, there was a positive control group and 2 (Trials 1 and 2) or 4 (Trials 3 and 4) additional treatment groups supplemented with additives, which were added to feed or water only to the finisher (21 to 42 d) diet (Trials 1 and 2) or to the starter (0 to 21 d) and finisher diets (Trials 3 and 4). Feed and water were available ad libitum. On days 35 and 42 of age in Trials 1 and 2, and on days 21, 35 and 42 of age in Trials 3 and 4, 10 (Trials 1 and 2) or 12 birds (Trials 3 and 4) per group were euthanized for cecal sampling. In Trial 1, birds fed with MCFA and MG-MCFA had a significant (P<0.05) reduction in cecal Campylobacter colonization compared to control at 35 d, but only the group treated with MG-MCFA maintained the reduction at 42 d. In Trials 2 to 4, no significant differences (P>0.05) in cecal Campylobacter counts were found between the treated and control animals. In conclusion, although none of the treatments were able to completely prevent the colonization of chickens with C. jejuni, MCFA and MG-MCFAs could reduce the pathogen counts when supplemented from 21 days onwards.
authors.affiliation: Imasde Agroalimentaria, S.L., 28224 Pozuelo de Alarcón, Madrid, Spain mgracia@e-imasde.com.
authors.forename: M I
authors.initials: MI
authors.lastname: Gracia
authors.affiliation: Imasde Agroalimentaria, S.L., 28224 Pozuelo de Alarcón, Madrid, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Millán
authors.affiliation: Imasde Agroalimentaria, S.L., 28224 Pozuelo de Alarcón, Madrid, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Sánchez
authors.affiliation: ANSES, Ploufragan-Plouzané Laboratory, Unit of Hygiene and Quality of Poultry and Pork Products, BP53, 22440 Ploufragan, France.
authors.forename: M
authors.initials: M
authors.lastname: Guyard-Nicodème
authors.affiliation: Fédération des Industries Avicoles, Paris, France.
authors.forename: J
authors.initials: J
authors.lastname: Mayot
authors.affiliation: Comité Interprofessionnel de la Dinde Française, Rennes, France.
authors.forename: Y
authors.initials: Y
authors.lastname: Carre
authors.affiliation: Baromfi Termék Tanács, Budapest, Hungary.
authors.forename: A
authors.initials: A
authors.lastname: Csorbai
authors.affiliation: ANSES, Ploufragan-Plouzané Laboratory, Unit of Hygiene and Quality of Poultry and Pork Products, BP53, 22440 Ploufragan, France.
authors.forename: M
authors.initials: M
authors.lastname: Chemaly
authors.affiliation: Imasde Agroalimentaria, S.L., 28224 Pozuelo de Alarcón, Madrid, Spain.
authors.forename: P
authors.initials: P
authors.lastname: Medel
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000818:Animals; D002169:Campylobacter Infections / Q000188:drug therapy / Q000382:microbiology / Q000662:veterinary*; D016123:Campylobacter jejuni / Q000187:drug effects*; D002432:Cecum / Q000382:microbiology; D002645:Chickens; D004032:Diet / Q000662:veterinary; D019587:Dietary Supplements / Q000032:analysis; D005260:Female; D008297:Male; D011201:Poultry Diseases / Q000188:drug therapy* / Q000382:microbiology
keywords: CAMPYBRO; Campylobacter jejuni; broiler; feed additive