title: Group A Streptococcus emm3 strains induce early macrophage cell death.
abstract: Group A Streptococcus (GAS) infections present high morbidity and mortality rates and consequently remain a significant health problem. The emm3 isolates induce more severe pathologies than all others. In this study, we tested, on a collection of invasive and non-invasive emm3 clinical isolates, whether in that genotype the invasive status of the strains affects the innate immune response. We show that phagocytosis is dependent on the invasiveness of the isolates. Interestingly, all emm3 isolates compromise macrophage integrity, already noticeable 1 h after infection. Inflammatory modulators (IL-6, TNF-α and IFN-β) are nevertheless detected during at least 6 h post-infection. This is a likely consequence of the macrophages not being all infected. The efficient and rapid induction of macrophage death could explain the virulence of the emm3 strains.
authors.affiliation: INSERM U 1016, Institut Cochin, Unité FRM 'Barrières et Pathogènes' F-75014 Paris, France CNRS UMR 8104, F-75014 Paris, France Université Paris Descartes, Sorbonne Paris Cité, F-75014 Paris, France.
authors.forename: Márcia
authors.initials: M
authors.lastname: Dinis
authors.affiliation: INSERM U 1016, Institut Cochin, Unité FRM 'Barrières et Pathogènes' F-75014 Paris, France CNRS UMR 8104, F-75014 Paris, France Université Paris Descartes, Sorbonne Paris Cité, F-75014 Paris, France Centre National de Référence des Streptocoques, F-75014 Paris, France Hôpitaux Universitaires Paris Centre, Assistance Publique Hôpitaux de Paris, F-75014 Paris, France.
authors.forename: Céline
authors.initials: C
authors.lastname: Plainvert
authors.affiliation: INSERM U 1016, Institut Cochin, Unité FRM 'Barrières et Pathogènes' F-75014 Paris, France CNRS UMR 8104, F-75014 Paris, France Université Paris Descartes, Sorbonne Paris Cité, F-75014 Paris, France.
authors.forename: Magalie
authors.initials: M
authors.lastname: Longo
authors.affiliation: INSERM U 1016, Institut Cochin, Unité FRM 'Barrières et Pathogènes' F-75014 Paris, France CNRS UMR 8104, F-75014 Paris, France Université Paris Descartes, Sorbonne Paris Cité, F-75014 Paris, France.
authors.forename: Julie
authors.initials: J
authors.lastname: Guignot
authors.affiliation: INSERM U 1016, Institut Cochin, Unité FRM 'Barrières et Pathogènes' F-75014 Paris, France CNRS UMR 8104, F-75014 Paris, France Université Paris Descartes, Sorbonne Paris Cité, F-75014 Paris, France.
authors.forename: Christelle
authors.initials: C
authors.lastname: Gabriel
authors.affiliation: INSERM U 1016, Institut Cochin, Unité FRM 'Barrières et Pathogènes' F-75014 Paris, France CNRS UMR 8104, F-75014 Paris, France Université Paris Descartes, Sorbonne Paris Cité, F-75014 Paris, France Centre National de Référence des Streptocoques, F-75014 Paris, France Hôpitaux Universitaires Paris Centre, Assistance Publique Hôpitaux de Paris, F-75014 Paris, France.
authors.forename: Claire
authors.initials: C
authors.lastname: Poyart
authors.affiliation: INSERM U 1016, Institut Cochin, Unité FRM 'Barrières et Pathogènes' F-75014 Paris, France CNRS UMR 8104, F-75014 Paris, France Université Paris Descartes, Sorbonne Paris Cité, F-75014 Paris, France Centre National de Référence des Streptocoques, F-75014 Paris, France agnes.fouet@inserm.fr.
authors.forename: Agnès
authors.initials: A
authors.lastname: Fouet
mesh_terms: D000818:Animals; D053148:Caspase 3 / Q000378:metabolism; D016923:Cell Death / Q000276:immunology; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D018836:Inflammation Mediators / Q000378:metabolism; D008264:Macrophages / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology*; D051379:Mice; D010587:Phagocytosis / Q000276:immunology; D013290:Streptococcal Infections / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology*; D013297:Streptococcus pyogenes / Q000145:classification / Q000302:isolation & purification / Q000472:pathogenicity / Q000502:physiology*; D014774:Virulence
chemical_list: D016207:Cytokines; D018836:Inflammation Mediators; D053148:Caspase 3
keywords: Streptococcus pyogenes; cell death; cytokine; emm genotype; macrophages; virulence