title: Disruption of OsEXO70A1 Causes Irregular Vascular Bundles and Perturbs Mineral Nutrient Assimilation in Rice.
abstract: Normal uptake, transportation, and assimilation of primary nutrients are essential to plant growth. Tracheary elements (TEs) are tissues responsible for the transport of water and minerals and characterized by patterned secondary cell wall (SCW) thickening. Exocysts are involved in the regulation of SCW deposition by mediating the targeted transport of materials and enzymes to specific membrane areas. EXO70s are highly duplicated in plants and provide exocysts with functional specificity. In this study, we report the isolation of a rice mutant rapid leaf senescence2 (rls2) that exhibits dwarfism, ferruginous spotted necrotic leaves, decreased hydraulic transport, and disordered primary nutrient assimilation. Histological analysis of rls2-1 mutants has indicated impaired cell expansion, collapsed vascular tissues, and irregular SCW deposition. Map-based cloning has revealed that RLS2 encodes OsEXO70A1, which is one of the 47 members of EXO70s in rice. RLS2 was widely expressed and spatially restricted in vascular bundles. Subcellular localization analysis demonstrated that RLS2 was present on both membrane and nuclear regions. Expression analysis revealed that mutations in rls2 triggers transcriptional fluctuation of orthologous EXO70 genes and affects genes involved in primary nutrient absorption and transport. In brief, our study revealed that RLS2 is required for normal vascular bundle differentiation and primary nutrient assimilation.
authors.affiliation: Rice Research Institute of Sichuan Agricultural University, Chengdu Wenjiang, Sichuan, 611130, PR China.|State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu Wenjiang, Sichuan, 611130, PR China.
authors.forename: Bin
authors.initials: B
authors.lastname: Tu
authors.affiliation: Rice Research Institute of Sichuan Agricultural University, Chengdu Wenjiang, Sichuan, 611130, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Hu
authors.affiliation: Rice Research Institute of Sichuan Agricultural University, Chengdu Wenjiang, Sichuan, 611130, PR China.|State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu Wenjiang, Sichuan, 611130, PR China.
authors.forename: Weilan
authors.initials: W
authors.lastname: Chen
authors.affiliation: Rice Research Institute of Sichuan Agricultural University, Chengdu Wenjiang, Sichuan, 611130, PR China.
authors.forename: Tao
authors.initials: T
authors.lastname: Li
authors.affiliation: Rice Research Institute of Sichuan Agricultural University, Chengdu Wenjiang, Sichuan, 611130, PR China.
authors.forename: Binhua
authors.initials: B
authors.lastname: Hu
authors.affiliation: Hybrid rice research center of Neijiang academy of agricultural, Neijiang, Sichuan, 641000, PR China.
authors.forename: Ling
authors.initials: L
authors.lastname: Zheng
authors.affiliation: Rice Research Institute of Sichuan Agricultural University, Chengdu Wenjiang, Sichuan, 611130, PR China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Lv
authors.affiliation: Rice Research Institute of Sichuan Agricultural University, Chengdu Wenjiang, Sichuan, 611130, PR China.
authors.forename: Shuju
authors.initials: S
authors.lastname: You
authors.affiliation: Rice Research Institute of Sichuan Agricultural University, Chengdu Wenjiang, Sichuan, 611130, PR China.|State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu Wenjiang, Sichuan, 611130, PR China.
authors.forename: Yuping
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Rice Research Institute of Sichuan Agricultural University, Chengdu Wenjiang, Sichuan, 611130, PR China.
authors.forename: Bingtian
authors.initials: B
authors.lastname: Ma
authors.affiliation: Rice Research Institute of Sichuan Agricultural University, Chengdu Wenjiang, Sichuan, 611130, PR China.
authors.forename: Xuewei
authors.initials: X
authors.lastname: Chen
authors.affiliation: Rice Research Institute of Sichuan Agricultural University, Chengdu Wenjiang, Sichuan, 611130, PR China.|State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu Wenjiang, Sichuan, 611130, PR China.
authors.forename: Peng
authors.initials: P
authors.lastname: Qin
authors.affiliation: Rice Research Institute of Sichuan Agricultural University, Chengdu Wenjiang, Sichuan, 611130, PR China.|State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu Wenjiang, Sichuan, 611130, PR China.
authors.forename: Shigui
authors.initials: S
authors.lastname: Li
mesh_terms: D000595:Amino Acid Sequence; D002473:Cell Wall / Q000378:metabolism; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D058685:Genetic Pleiotropy; D008903:Minerals / Q000378:metabolism*; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics*; D009928:Organ Specificity; D012275:Oryza / Q000235:genetics* / Q000648:ultrastructure; D010641:Phenotype; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D058526:Plant Vascular Bundle / Q000378:metabolism* / Q000648:ultrastructure; D013347:Subcellular Fractions / Q000378:metabolism; D014158:Transcription, Genetic; D014867:Water
chemical_list: D008903:Minerals; D010940:Plant Proteins; D014867:Water