title: Effectiveness of fluoride sealant in the prevention of carious lesions around orthodontic brackets: an OCT evaluation.
abstract: OBJECTIVE
  This article aimed to evaluate in vitro the efficiency of Pro Seal fluoride sealant application in the prevention of white spot lesions around orthodontic brackets.
  
  
  METHODS
  Brackets were bonded to the buccal surface of bovine incisors, and five groups were formed (n = 15) according to the exposure of teeth to oral hygiene substances and the application of enamel sealant: G1 (control), only brushing was performed with 1.450 ppm fluoride; G2 (control) brushing associated with the use of mouthwash with 225 ppm fluoride; G3, only Pro Seal sealant application was performed with 1.000 ppm fluoride; G4 Pro Seal associated with brushing; G5 Pro Seal associated with brushing and mouthwash. Experimental groups alternated between pH cycling and the procedures described. All specimens were kept at a temperature of 37 °C throughout the entire experiment. Both brushing and immersion in solutions were performed within a time interval of one minute, followed by washing in deionized water three times a day for 28 days. Afterwards, an evaluation by Optical Coherence Tomography (OCT) of the spectral type was performed. In each group, a scanning exam of the white spot lesion area (around the sites where brackets were bonded) and depth measurement of carious lesions were performed. Analysis of variance (ANOVA) was applied to determine whether there were significant differences among groups. For post hoc analysis, Tukey test was used.
  
  
  RESULTS
  There was statistically significant difference between groups 1 and 2 (p = 0.003), 1 and 3 (p = 0.008), 1 and 4 (p = 0.000) and 1 and 5 (p = 0.000). The group in which only brushing was performed (Group 1) showed deeper enamel lesion.
  
  
  CONCLUSIONS
  Pro Seal sealant alone or combined with brushing and/or brushing and the use of a mouthwash with fluoride was more effective in protecting enamel, in comparison to brushing alone.
authors.affiliation: Department of Health, School of Dentistry, Universidade Estadual do Sudoeste da Bahia, Jequié, Bahia, Brazil.
authors.forename: Matheus Melo
authors.initials: MM
authors.lastname: Pithon
authors.affiliation: Department of Health, School of Dentistry, Universidade Estadual do Sudoeste da Bahia, Jequié, Bahia, Brazil.
authors.forename: Mariana de Jesus
authors.initials: Mde J
authors.lastname: Santos
authors.affiliation: Department of Health, School of Dentistry, Universidade Estadual do Sudoeste da Bahia, Jequié, Bahia, Brazil.
authors.forename: Camilla Andrade
authors.initials: CA
authors.lastname: de Souza
authors.affiliation: School of Health and Biosciences, Pontifícia Universidade Católica do Paraná, Curitiba, Paraná, Brazil.
authors.forename: Jorge César Borges
authors.initials: JC
authors.lastname: Leão Filho
authors.affiliation: Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil.
authors.forename: Ana Karla Souza
authors.initials: AK
authors.lastname: Braz
authors.affiliation: Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil.
authors.forename: Renato Evangelista
authors.initials: RE
authors.lastname: de Araujo
authors.affiliation: Pontifícia Universidade Católica do Paraná, Curitiba, Paraná, Brazil.
authors.forename: Orlando Motohiro
authors.initials: OM
authors.lastname: Tanaka
authors.affiliation: Pontifícia Universidade Católica de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Dauro Douglas
authors.initials: DD
authors.lastname: Oliveira
mesh_terms: D000818:Animals; D002417:Cattle; D003743:Dental Enamel; D003764:Dental Materials; D005459:Fluorides; D016910:Orthodontic Brackets; D014099:Toothbrushing
chemical_list: D003764:Dental Materials; D005459:Fluorides