title: Influence of the crystallinity of a sputtered hydroxyapatite film on its osteocompatibility.
abstract: Hydroxyapatite (HA) was coated onto titanium substrates using radio frequency sputtering, and the sputtered films were crystallized using a hydrothermal treatment at 120°C and 170°C to evaluate the influence of the crystallinity of the HA film on its osteocompatibility. The crystallite size and surface morphology of the films were observed using X-ray diffraction (XRD) and scanning electron microscopy (SEM), respectively. The alkaline phosphatase (ALP) expression, osteocalin (OCN) expression and bone formation of osteoblast cells on the films were measured to evaluate the osteocompatibility of the film.The crystallite size increased as the hydrothermal temperature increased, and the crystallite sizes of the film treated at 120°C and 170°C were 82.2±12.3 nm and 124.7±13.3 nm, respectively. Globular particles were observed in the hydrothermally treated film using SEM. The size of the particles on the film increased as the hydrothermal temperature increased, and the width of the particles on the film treated at 120°C and 170°C were approximately 120-190 nm and 300-500 nm, respectively. In the osteoblast cell culture experiments, the ALP expression, OCN expression and bone formation area on the films treated at 120°C were higher than those treated for films treated at 170°C.
authors.affiliation: Department of Mechanical Engineering, Ibaraki University, 4-12-1, Nakanarusawa, Hitachi, Ibaraki, 316-8511, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Ozeki
authors.affiliation: Department of Anatomy of Oral Science, Graduate School of Medical and Dental Science, Kagoshima University, 8-35-1, Sakuragaoka, Kagoshima, 890-8544, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Goto
authors.affiliation: International Apatite Co., Ltd, 20 Kanda-Ogawamachi 3-Chome, Chiyoda-ku, Tokyo, 101-0052, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Aoki
authors.affiliation: Department of Mechanical Engineering, Ibaraki University, 4-12-1, Nakanarusawa, Hitachi, Ibaraki, 316-8511, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Masuzawa
mesh_terms: D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D003460:Crystallization; D017886:Durapatite / Q000737:chemistry*; D005786:Gene Expression Regulation; D006358:Hot Temperature; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects*; D015675:Osteocalcin / Q000235:genetics / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects*; D051381:Rats; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry; D014961:X-Ray Diffraction
chemical_list: D020099:Coated Materials, Biocompatible; D015675:Osteocalcin; D017886:Durapatite; D014025:Titanium; D000469:Alkaline Phosphatase
keywords: Hydroxyapatite; crystallinity; osteoblast cell; sputtering