title: Investigation of polymorphisms in anti-inflammatory cytokine genes in hematogenous osteomyelitis.
abstract: Osteomyelitis is a progressive bone infection disease caused by destructive immunological inflammatory reactions following new bone formation. Anti-inflammatory cytokines are a series of immunoregulatory molecules that control the pro-inflammatory cytokine response. In this study, we investigated 9 single nucleotide polymorphisms in 5 different cytokine/cytokine receptor genes in hematogenous osteomyelitis (HO) patients, and compared their outcomes with normal healthy individuals. Sequence-specific forward and reverse primers and two TaqMan® MGB probes with dyes (VIC™ and FAM™) that specifically detect Allele 1 and Allele 2 of each SNP were utilized. The genotypes CC (P = 0.009) and CT (P = 0.041) of SNP rs2070874, and alleles A (P = 0.044) and G of SNP rs1800871 were significantly different between the patients and healthy controls. The expression of the CC genotype or C allele at rs2070874 was a risk factor for HO development, with higher frequencies of CT and T being found in the control samples. The expression of the A allele of rs1800871 was also significantly higher in patients than in controls, and was therefore considered a risk factor.
authors.affiliation: King Fahad Medical City, Riyadh, Saudi Arabia.
authors.forename: A E
authors.initials: AE
authors.lastname: Osman
authors.affiliation: King Fahad Medical City, Riyadh, Saudi Arabia.
authors.forename: M
authors.initials: M
authors.lastname: Mubasher
authors.affiliation: King Fahad Medical City, Riyadh, Saudi Arabia.
authors.forename: N E
authors.initials: NE
authors.lastname: ElSheikh
authors.affiliation: King Fahad Medical City, Riyadh, Saudi Arabia.
authors.forename: H
authors.initials: H
authors.lastname: AlHarthi
authors.affiliation: King Fahad Medical City, Riyadh, Saudi Arabia.
authors.forename: I A
authors.initials: IA
authors.lastname: AlAlallah
authors.affiliation: King Fahad Medical City, Riyadh, Saudi Arabia.
authors.forename: A A
authors.initials: AA
authors.lastname: Elbeshir
authors.affiliation: King Fahad Medical City, Riyadh, Saudi Arabia.
authors.forename: M
authors.initials: M
authors.lastname: Abashar
authors.affiliation: King Fahad Medical City, Riyadh, Saudi Arabia.
authors.forename: N
authors.initials: N
authors.lastname: Elsidig
authors.affiliation: Mafraq Hospital and Sheikh Khalifa Medical City, Abu Dhabi, United Arab Emirates.
authors.forename: G
authors.initials: G
authors.lastname: ElGhazali
authors.affiliation: King Fahad Medical City, Riyadh, Saudi Arabia.
authors.forename: A-S A
authors.initials: AS
authors.lastname: Fadil
mesh_terms: D000293:Adolescent; D000483:Alleles; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D016207:Cytokines / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D007223:Infant; D008297:Male; D016017:Odds Ratio; D010019:Osteomyelitis / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide
chemical_list: D016207:Cytokines