title: [Preliminary study on effect of Rhodiolae Crenulatae Radix et Rhizoma cell wall-broken decoction pieces on intestinal flora of mice].
abstract: This study aims to analyze and compare the effect of cell wall-broken decoction pieces, conventional decoction pieces and conventional powder of Rhodiolae Crenulatae Radix et Rhizoma on the intestinal flora of normal mice. The conventional bacterial culture and PCR-DGGE (polymerase chain reaction-denaturing gradient gel electrophoresis) were adopted for the mice after the oral administration for 14 days. According to the bacterial culture results, the 1/8 dose cell wall-broken decoction pieces group showed fewer Enterococcus and Escherichia coli bacillus but more Lactobacillus and Bifidobacterium than the conventional decoction pieces group and the traditional powder group (P <0.05). Meanwhile, on the basis of the PCR-DGGE results, the 1/8 dose cell wall-broken decoction pieces group revealed the highest Shannon-Wiener index (H) and species richness (S) among the seven groups, with extremely significant differences compared with the normal group (P <0.01), significant differences compared with the conventional decoction pieces group and the conventional powder group (P <0.05) and a high intra-group similarity. In conclusion, the long-term intake of 1/8 dose Rhodiolae Crenulatae Radix et Rhizoma cell wall-broken decoction pieces showed a certain effect in regulating intestinal tract by promoting the growth of Lactobacillus and Bifidobacterium. Furthermore, the intestinal flora community will become more stable.
authors.forename: Ze-rui
authors.initials: ZR
authors.lastname: Yang
authors.forename: Gui-mei
authors.initials: GM
authors.lastname: Zeng
authors.forename: Li-hua
authors.initials: LH
authors.lastname: Peng
authors.forename: Miao-miao
authors.initials: MM
authors.lastname: Zhang
authors.forename: Jin-le
authors.initials: JL
authors.lastname: Cheng
authors.forename: Ruo-ting
authors.initials: RT
authors.lastname: Zhan
mesh_terms: D000818:Animals; D001644:Bifidobacterium / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D002473:Cell Wall; D058645:Denaturing Gradient Gel Electrophoresis; D007422:Intestines / Q000382:microbiology*; D007778:Lactobacillus / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D051379:Mice; D008810:Mice, Inbred C57BL; D016133:Polymerase Chain Reaction; D027343:Rhizome; D029771:Rhodiola