title: Cholesterol esterase inhibitory activity of bioactives from leaves of Mangifera indica L.
abstract: BACKGROUND
  In the earlier studies, methanolic extract of Mangifera indica L leaf was exhibited hypocholesterol activity. However, the bioactive compounds responsible for the same are not reported so far.
  
  
  OBJECTIVE
  To isolate the bioactive compounds with hypocholesterol activity from the leaf extract using cholesterol esterase inhibition assay which can be used for the standardization of extract.
  
  
  METHODS
  The leaf methanolic extract of M. indica (Sindoora variety) was partitioned with ethyl acetate and chromatographed on silica gel to yield twelve fractions and the activity was monitored by using cholesterol esterase inhibition assay. Active fractions were re-chromatographed to yield individual compounds.
  
  
  CONCLUSIONS
  A major compound mangiferin present in the extract was screened along with other varieties of mango leaves for cholesterol esterase inhibition assay. However, the result indicates that compounds other than mangiferin may be active in the extract. Invitro pancreatic cholesterol esterase inhibition assay was used for bioactivity guided fractionation (BAGF) to yield bioactive compound for standardization of extract. Bioactivity guided fractionation afford the active fraction containing 3b-taraxerol with an IC50 value of 0.86μg/ml.
  
  
  CONCLUSIONS
  This study demonstrates that M. indica methanol extract of leaf have significant hypocholesterol activity which is standardized with 3b-taraxerol, a standardized extract for hypocholesterol activity resulted in development of dietary supplement from leaves of Mangifera indica.
authors.affiliation: Research Scholar, Manipal University, Manipal, India ; R & D Centre, Natural Remedies Pvt Ltd, Bengaluru, India.
authors.forename: G M
authors.initials: GM
authors.lastname: Gururaja
authors.affiliation: R & D Centre, Natural Remedies Pvt Ltd, Bengaluru, India.
authors.forename: Deepak
authors.initials: D
authors.lastname: Mundkinajeddu
authors.affiliation: R & D Centre, Natural Remedies Pvt Ltd, Bengaluru, India.
authors.forename: Shekhar M
authors.initials: SM
authors.lastname: Dethe
authors.affiliation: R & D Centre, Natural Remedies Pvt Ltd, Bengaluru, India.
authors.forename: Gopala K
authors.initials: GK
authors.lastname: Sangli
authors.affiliation: R & D Centre, Natural Remedies Pvt Ltd, Bengaluru, India.
authors.forename: K
authors.initials: K
authors.lastname: Abhilash
authors.affiliation: R & D Centre, Natural Remedies Pvt Ltd, Bengaluru, India.
authors.forename: Amit
authors.initials: A
authors.lastname: Agarwal
keywords: 3 β-taraxerol; Bioactivity; Hypocholesterol; Mangifera indica