title: Anti-inflammatory potential of β-cryptoxanthin against LPS-induced inflammation in mouse Sertoli cells.
abstract: β-cryptoxanthin (CX), a major carotenoid pigment, can inhibit inflammatory gene expression in mice with nonalcoholic steatohepatitis. In the present study, we examined the anti-inflammatory effects of CX on lipopolysaccharide (LPS)-induced inflammation in mouse primary Sertoli cells and the possible molecular mechanisms behind its effects. The results showed that CX significantly inhibited LPS-induced decreases in cell viability and in the percentage of apoptotic cells. Moreover, CX inhibited the LPS-induced up-regulation of tumor necrosis factor α (TNF-α), interleukin-10 (IL-10), interleukin-6 (IL-6) and interleukin-1β (IL-1β) in Sertoli cells. In addition, CX significantly limited the LPS-induced down-regulation of AR, HSF2, CREB, FSHR, INHBB and ABP in Sertoli cells. Western blot analysis showed that CX significantly suppressed NF-κB (p65) activation as well as MAPK phosphorylation. All the results suggested that CX suppressed inflammation, possibly associated with the NF-κB activation and MAPK of phosphorylation. Thus, CX may possess therapeutic potential against inflammation-related diseases.
authors.affiliation: Key Laboratory of China Education Ministry in Agricultural Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, P.R. China, 430070.
authors.forename: Xiao-Ran
authors.initials: XR
authors.lastname: Liu
authors.affiliation: Key Laboratory of China Education Ministry in Agricultural Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, P.R. China, 430070.
authors.forename: Yue-Ying
authors.initials: YY
authors.lastname: Wang
authors.affiliation: Key Laboratory of China Education Ministry in Agricultural Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, P.R. China, 430070.
authors.forename: Xin-Gang
authors.initials: XG
authors.lastname: Dan
authors.affiliation: College of Life Science and Technology, State Key laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, P.R. China, 430070.
authors.forename: Ashok
authors.initials: A
authors.lastname: Kumar
authors.affiliation: Key Laboratory of China Education Ministry in Agricultural Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, P.R. China, 430070.
authors.forename: Ting-Zhu
authors.initials: TZ
authors.lastname: Ye
authors.affiliation: Key Laboratory of China Education Ministry in Agricultural Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, P.R. China, 430070.
authors.forename: Yao-Yao
authors.initials: YY
authors.lastname: Yu
authors.affiliation: Key Laboratory of China Education Ministry in Agricultural Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, P.R. China, 430070. Electronic address: yangliguo2006@qq.com.
authors.forename: Li-Guo
authors.initials: LG
authors.lastname: Yang
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D000072743:Beta-Cryptoxanthin / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D016207:Cytokines / Q000235:genetics; D008070:Lipopolysaccharides; D008297:Male; D051379:Mice; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D012333:RNA, Messenger / Q000378:metabolism; D012708:Sertoli Cells / Q000187:drug effects* / Q000378:metabolism; D013091:Spermatogenesis / Q000235:genetics
chemical_list: D000893:Anti-Inflammatory Agents; D000072743:Beta-Cryptoxanthin; D016207:Cytokines; D008070:Lipopolysaccharides; D016328:NF-kappa B; D012333:RNA, Messenger; D020928:Mitogen-Activated Protein Kinases
keywords: Inflammatory cytokines; Lipopolysaccharide; Mitogen-activated protein kinases; Sertoli cell; β-cryptoxanthin