title: Biotechnological Production of Docosahexaenoic Acid Using Aurantiochytrium limacinum: Carbon Sources Comparison And Growth Characterization.
abstract: Aurantiochytrium limacinum, a marine heterotrophic protist/microalga has shown interesting yields of docosahexaenoic acid (DHA) when cultured with different carbon sources: glucose, pure and crude glycerol. A complete study in a lab-scale fermenter allowed for the characterization and comparison of the growth kinetic parameters corresponding to each carbon source. Artificial Marine Medium (AMM) with glucose, pure and crude glycerol offered similar biomass yields. The net growth rates (0.10-0.12 h(-1)), biomass (0.7-0.8 g cells/g Substrate) and product (0.14-0.15 g DHA/g cells) yields, as well as DHA productivity were similar using the three carbon sources. Viable potential applications to valorize crude glycerol are envisioned to avoid an environmental problem due to the excess of byproduct.
authors.affiliation: Bioengineering Department, IQS, Ramon Llull University, Via Augusta 390, Barcelona 08017, Spain. sergi.abad@iqs.edu.
authors.forename: Sergi
authors.initials: S
authors.lastname: Abad
authors.affiliation: Bioengineering Department, IQS, Ramon Llull University, Via Augusta 390, Barcelona 08017, Spain. xavier.turon@iqs.edu.
authors.forename: Xavier
authors.initials: X
authors.lastname: Turon
mesh_terms: D018533:Biomass; D019149:Bioreactors; D001709:Biotechnology / Q000379:methods*; D002244:Carbon / Q000737:chemistry / Q000378:metabolism*; D046508:Culture Techniques; D004281:Docosahexaenoic Acids / Q000096:biosynthesis*; D005947:Glucose / Q000737:chemistry / Q000378:metabolism; D005990:Glycerol / Q000737:chemistry / Q000378:metabolism; D058009:Stramenopiles / Q000378:metabolism*
chemical_list: D004281:Docosahexaenoic Acids; D002244:Carbon; D005947:Glucose; D005990:Glycerol
keywords: DHA; PUFA; biodiesel; bioprocess; fatty acids; glycerol; microalgae; nutraceutics; omega-3 fatty acids