title: Nanoporous Glass Integrated in Volumetric Bar-Chart Chip for Point-of-Care Diagnostics of Non-Small Cell Lung Cancer.
abstract: Point-of-care (POC) testing has the potential to enable rapid, low-cost, and large-scale screening. POC detection of a multiplexed biomarker panel can facilitate the early diagnosis of non-small cell lung cancer (NSCLC) and, thus, may allow for more timely surgical intervention for life-saving treatment. Herein, we report the nanoporous glass (NPG) integrated volumetric bar-chart chip (V-Chip) for POC detection of the three NSCLC biomarkers CEA, CYFRA 21-1, and SCCA, by the naked eye. The 3D nanostructures in the NPG membrane efficiently increase the number of binding sites for antibodies and decrease the diffusion distance between antibody and antigen, enabling the low detection limit and rapid analysis time of the NPG-V-Chip. We utilized the NPG-V-Chip to test the NSCLC biomarker panel and found that the limit of detection can reach 50 pg/mL (10-fold improvement over the original V-Chip), and the total assay time can be decreased from 4 to 0.5 h. We then detected CEA in 21 serum samples from patients with common cancers, and the on-chip results showed good correlation with the clinical results. We further assayed 10 lung cancer samples using the device and confirmed the results obtained using conventional ELISA methods. In summary, the NPG-V-Chip platform has the ability of multiplex, low detection limit, low cost, lack of need for accessory equipment, and rapid analysis time, which may render the V-Chip a useful platform for quantitative POC detection in resource-limited settings and personalized diagnostics.
authors.affiliation: Department of Nanomedicine, Houston Methodist Research Institute , 6670 Bertner Avenue, Houston, Texas 77030, United States.|Department of Cell and Developmental Biology, Weill Medical College of Cornell University , New York, New York 10065, United States.
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Pathology and Genomic Medicine, Houston Methodist Hospital , 6670 Bertner Avenue, Houston, Texas 77030, United States.
authors.forename: Jie
authors.initials: J
authors.lastname: Xuan
authors.affiliation: Department of Nanomedicine, Houston Methodist Research Institute , 6670 Bertner Avenue, Houston, Texas 77030, United States.|Department of Cell and Developmental Biology, Weill Medical College of Cornell University , New York, New York 10065, United States.
authors.forename: Yujun
authors.initials: Y
authors.lastname: Song
authors.affiliation: Department of Nanomedicine, Houston Methodist Research Institute , 6670 Bertner Avenue, Houston, Texas 77030, United States.|Department of Obstetrics & Gynecology, The First Affiliated Hospital of Kunming Medical University , Kunming 650032, China.
authors.forename: Wenjin
authors.initials: W
authors.lastname: Qi
authors.affiliation: Department of Nanomedicine, Houston Methodist Research Institute , 6670 Bertner Avenue, Houston, Texas 77030, United States.|Central Laboratory, Nanjing First Hospital, Nanjing Medical University , Nanjing 210006, China.
authors.forename: Bangshun
authors.initials: B
authors.lastname: He
authors.affiliation: Department of Pathology and Genomic Medicine, Houston Methodist Hospital , 6670 Bertner Avenue, Houston, Texas 77030, United States.
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.affiliation: Department of Nanomedicine, Houston Methodist Research Institute , 6670 Bertner Avenue, Houston, Texas 77030, United States.|Department of Cell and Developmental Biology, Weill Medical College of Cornell University , New York, New York 10065, United States.
authors.forename: Lidong
authors.initials: L
authors.lastname: Qin
mesh_terms: D000912:Antibodies, Neoplasm / Q000032:analysis*; D000936:Antigen-Antibody Complex / Q000032:analysis; D000951:Antigens, Neoplasm / Q000235:genetics* / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000235:genetics* / Q000378:metabolism; D002272:Carcinoembryonic Antigen / Q000235:genetics* / Q000378:metabolism; D002289:Carcinoma, Non-Small-Cell Lung / Q000175:diagnosis* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D042241:Early Diagnosis; D015870:Gene Expression; D005898:Glass / Q000737:chemistry; D006801:Humans; D053539:Keratin-19 / Q000235:genetics* / Q000378:metabolism; D056656:Lab-On-A-Chip Devices; D057230:Limit of Detection; D008175:Lung Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D049329:Nanostructures / Q000737:chemistry; D019095:Point-of-Care Systems; D016062:Porosity; D015843:Serpins / Q000235:genetics* / Q000378:metabolism
chemical_list: D000912:Antibodies, Neoplasm; D000936:Antigen-Antibody Complex; D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D002272:Carcinoembryonic Antigen; D053539:Keratin-19; D015843:Serpins; C102012:antigen CYFRA21.1; C051072:squamous cell carcinoma-related antigen
keywords: NSCLC; V-Chip; biomarker detection; microfluidics; nanoporous glass; point-of-care testing