title: Selective conversion of carbon monoxide to hydrogen by anaerobic mixed culture.
abstract: A new method for the conversion of CO to H2 was developed by anaerobic mixed culture in the current study. Higher CO consumption rate was obtained by anaerobic granular sludge (AGS) compared to waste activated sludge (WAS) at 55 °C and pH 7.5. However, H2 was the intermediate and CH4 was the final product. Fermentation at pH 5.5 by AGS inhibited CH4 production, while the lower CO consumption rate (50% of that at pH 7.5) and the production of acetate were found. Fermentation at pH 7.5 with the addition of chloroform achieved efficient and selective conversion of CO to H2. Stable and efficient H2 production was achieved in a continuous reactor inoculated with AGS, and gas recirculation was crucial to increase the CO conversion efficiency. Microbial community analysis showed that high abundance (44%) of unclassified sequences and low relative abundance (1%) of known CO-utilizing bacteria Desulfotomaculum were enriched in the reactor.
authors.affiliation: Shanghai Key Laboratory of Atmospheric Particle Pollution and Prevention (LAP(3)), Department of Environmental Science and Engineering, Fudan University, 200433 Shanghai, China; School of Chemical and Environmental Engineering, Shanghai Institute of Technology, 201418 Shanghai, China.
authors.forename: Yafeng
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Shanghai Key Laboratory of Atmospheric Particle Pollution and Prevention (LAP(3)), Department of Environmental Science and Engineering, Fudan University, 200433 Shanghai, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Wan
authors.affiliation: School of Chemical and Environmental Engineering, Shanghai Institute of Technology, 201418 Shanghai, China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Han
authors.affiliation: Shanghai Key Laboratory of Atmospheric Particle Pollution and Prevention (LAP(3)), Department of Environmental Science and Engineering, Fudan University, 200433 Shanghai, China.
authors.forename: Shicheng
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Shanghai Key Laboratory of Atmospheric Particle Pollution and Prevention (LAP(3)), Department of Environmental Science and Engineering, Fudan University, 200433 Shanghai, China. Electronic address: gangl@fudan.edu.cn.
authors.forename: Gang
authors.initials: G
authors.lastname: Luo
mesh_terms: D000693:Anaerobiosis; D001419:Bacteria / Q000378:metabolism*; D061249:Batch Cell Culture Techniques; D019149:Bioreactors / Q000382:microbiology; D002248:Carbon Monoxide / Q000378:metabolism*; D005285:Fermentation; D006859:Hydrogen / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D010313:Partial Pressure; D010802:Phylogeny; D012722:Sewage / Q000382:microbiology; D013997:Time Factors
chemical_list: D012722:Sewage; D002248:Carbon Monoxide; D006859:Hydrogen
keywords: CO; H(2); Microbial community; Mixed culture; Reactor performance