title: Microorganisms inhabiting follicular contents of facial acne are not only Propionibacterium but also Malassezia spp.
abstract: To clarify the relationship between major cutaneous microorganisms (Propionibacterium, Staphylococcus and Malassezia spp.) and acne vulgaris (acne), we examined the microbiota quantitatively in the follicular contents of inflammatory acne and on the facial skin of patients with acne. Fifteen Japanese untreated acne outpatients were studied. The follicular contents from inflammatory acne lesions of the face were collected using a comedo extractor. The skin surface samples were obtained by the swab method from 10 cm(2) of facial skin. The microbiota was analyzed using polymerase chain reaction. The microbiota in follicular contents was similar to that on the skin surface, namely, there were large populations of Propionibacterium spp., Staphylococcus spp. and Malassezia spp. Moreover, the number of Malassezia spp. on the skin surface was correlated with that of inflammatory acne and that in follicular contents. This study clarified that there are large populations of Propionibacterium spp., Staphylococcus spp. and Malassezia spp. in follicular contents. These results suggest the possibility that not only Propionibacterium acnes but also other cutaneous resident microorganisms are related to acne. Particularly, we considered that Malassezia spp. is closely related.
authors.affiliation: Research Laboratories, Nippon Menard Cosmetic, Nagoya, Japan.|Department of Dermatology, Fujita Health University School of Medicine, Toyoake, Aichi, Japan.
authors.forename: Narifumi
authors.initials: N
authors.lastname: Akaza
authors.affiliation: Department of Applied Cell and Regenerative Medicine, Fujita Health University School of Medicine, Toyoake, Aichi, Japan.
authors.forename: Hirohiko
authors.initials: H
authors.lastname: Akamatsu
authors.affiliation: Department of Dermatology, Fujita Health University School of Medicine, Toyoake, Aichi, Japan.
authors.forename: Shigeki
authors.initials: S
authors.lastname: Numata
authors.affiliation: Research Laboratories, Nippon Menard Cosmetic, Nagoya, Japan.
authors.forename: Shunji
authors.initials: S
authors.lastname: Yamada
authors.affiliation: Department of Dermatology, Fujita Health University School of Medicine, Toyoake, Aichi, Japan.
authors.forename: Akiko
authors.initials: A
authors.lastname: Yagami
authors.affiliation: Research Laboratories, Nippon Menard Cosmetic, Nagoya, Japan.
authors.forename: Satoru
authors.initials: S
authors.lastname: Nakata
authors.affiliation: Department of Dermatology, Fujita Health University School of Medicine, Toyoake, Aichi, Japan.
authors.forename: Kayoko
authors.initials: K
authors.lastname: Matsunaga
mesh_terms: D000152:Acne Vulgaris / Q000382:microbiology*; D000293:Adolescent; D000328:Adult; D002648:Child; D005145:Face / Q000382:microbiology; D005260:Female; D006801:Humans; D008290:Malassezia / Q000302:isolation & purification*; D008297:Male; D064307:Microbiota; D011424:Propionibacterium / Q000302:isolation & purification*; D012867:Skin / Q000382:microbiology; D013210:Staphylococcus / Q000302:isolation & purification; D055815:Young Adult
keywords: Malassezia; Propionibacterium; acne vulgaris; follicular content; microbiota