title: Relation between Endothelial Nitric Oxide Synthase Genotypes and Oxidative Stress Markers in Larynx Cancer.
abstract: Nitric oxide synthase (eNOS/NOS3) is responsible for the endothelial synthesis of nitric oxide (NO(•)). G894T polymorphism leads to the amino acid substitution from Glu298Asp that causes lower NOS3 activity and basal NO(•) production in NOS3 894T (298Asp) allele carriers compared with the GG homozygotes. NO(•) acts as an antioxidant protecting against Fenton's reaction which generates highly reactive hydroxyl radicals. Allelic variation of NOS3 may influence an individual's risk of laryngeal cancer (LC). In the current study we have examined the possible relationship between NOS3 G894T genotypes and various systemic oxidative damage markers such as protein carbonyl, advanced oxidation protein products, Cu, Zn-superoxide dismutase, thiol group fractions, and lipid hydroperoxides in LC patients. Genotyping was carried out by PCR-RFLP. In LC patients with TT genotype, Cu, Zn-superoxide dismutase activities and nonprotein thiol levels were significantly higher than the controls. In patients with GT and GG genotype, high levels of lipid hydroperoxides showed statistical significance when compared to controls. Our results indicate a potential relationship among G894T polymorphism of NOS3, and impaired redox homeostasis. Further studies are required to determine the role of NOS3 gene polymorphism and impaired plasma redox homeostasis.
authors.affiliation: Department of Medical Biochemistry, Cerrahpaşa Faculty of Medicine, İstanbul University, 34098 İstanbul, Turkey.
authors.forename: K
authors.initials: K
authors.lastname: Yanar
authors.affiliation: Department of Medical Biochemistry, Cerrahpaşa Faculty of Medicine, İstanbul University, 34098 İstanbul, Turkey.
authors.forename: U
authors.initials: U
authors.lastname: Çakatay
authors.affiliation: Department of Medical Biochemistry, Cerrahpaşa Faculty of Medicine, İstanbul University, 34098 İstanbul, Turkey.
authors.forename: S
authors.initials: S
authors.lastname: Aydın
authors.affiliation: Department of Otorhinolaryngology/Head and Neck Surgery, Haydarpaşa Numune Educational and Research Hospital, 34668 İstanbul, Turkey.
authors.forename: A
authors.initials: A
authors.lastname: Verim
authors.affiliation: Department of Medical Biochemistry, Cerrahpaşa Faculty of Medicine, İstanbul University, 34098 İstanbul, Turkey.
authors.forename: P
authors.initials: P
authors.lastname: Atukeren
authors.affiliation: Department of Molecular Medicine, Institute of Experimental Medicine, Istanbul University, 34393 İstanbul, Turkey.
authors.forename: N E
authors.initials: NE
authors.lastname: Özkan
authors.affiliation: Cerrahpaşa Faculty of Medicine, İstanbul University, 34098 Istanbul, Turkey.
authors.forename: K
authors.initials: K
authors.lastname: Karatoprak
authors.affiliation: Cerrahpaşa Faculty of Medicine, İstanbul University, 34098 Istanbul, Turkey.
authors.forename: T
authors.initials: T
authors.lastname: Cebe
authors.affiliation: Department of Molecular Medicine, Institute of Experimental Medicine, Istanbul University, 34393 İstanbul, Turkey.
authors.forename: S
authors.initials: S
authors.lastname: Turan
authors.affiliation: Department of Neuroscience, Institute of Experimental Medicine, Istanbul University, 34393 İstanbul, Turkey.
authors.forename: E
authors.initials: E
authors.lastname: Ozkök
authors.affiliation: Department of Molecular Medicine, Institute of Experimental Medicine, Istanbul University, 34393 İstanbul, Turkey.
authors.forename: G
authors.initials: G
authors.lastname: Korkmaz
authors.affiliation: Department of Molecular Medicine, Institute of Experimental Medicine, Istanbul University, 34393 İstanbul, Turkey.
authors.forename: C
authors.initials: C
authors.lastname: Cacına
authors.affiliation: Department of Molecular Medicine, Institute of Experimental Medicine, Istanbul University, 34393 İstanbul, Turkey.
authors.forename: O
authors.initials: O
authors.lastname: Küçükhüseyin
authors.affiliation: Department of Molecular Medicine, Institute of Experimental Medicine, Istanbul University, 34393 İstanbul, Turkey.
authors.forename: İ
authors.initials: İ
authors.lastname: Yaylım
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism; D016022:Case-Control Studies; D005260:Female; D005838:Genotype; D006801:Humans; D007822:Laryngeal Neoplasms / Q000201:enzymology / Q000235:genetics; D008297:Male; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism; D052250:Nitric Oxide Synthase Type III / Q000235:genetics / Q000378:metabolism; D018384:Oxidative Stress; D011110:Polymorphism, Genetic
chemical_list: D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins; C496314:NOS3 protein, human; D052250:Nitric Oxide Synthase Type III