title: Development of a QuEChERS-based extraction method for the determination of destruxins in potato plants by UHPLC-MS/MS.
abstract: Ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) with electrospray ionization has been proposed for the determination of fifteen natural destruxins (A, B, C, D, E, Ed, Ed1, A2, B2, D2, E2, Cl, DesmA, DesmB, and DH-A), secondary metabolites with insecticidal and phytotoxic activities produced by Metarhizium species fungus, which are being studied as biological agents in pest control. Therefore, procedures to control them in the food chain are required, starting with crops. As a consequence, in this study, a simple QuEChERS-based destruxin (dtx) extraction procedure has been developed and validated in four different parts of potato plant (tuber, root, stem and leaves) for the first time. For dtx A, the limits of detection obtained, ranged between 0.5 and 1.3 μg/kg, and for quantification, ranged between 1.7 and 4.2 μg/kg. Precision values were below 8.5%; and in all cases, recoveries were higher than 91%. Finally, the method has been applied in potato samples inoculated by EAMa 01/58-Su strain, where dtxs A and B were detected and quantified. In all cases, dtx B concentration was higher than dtx A.
authors.affiliation: Department of Analytical Chemistry, University of Cordoba, Annex C3 Building, Campus of Rabanales, 14071 Cordoba, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Carpio
authors.affiliation: Department of Analytical Chemistry, University of Granada, Campus Fuentenueva s/n, 18071 Granada, Spain.
authors.forename: N
authors.initials: N
authors.lastname: Arroyo-Manzanares
authors.affiliation: Department of Agricultural and Forestry Sciences, ETSIAM, University of Cordoba, C4 Building, Campus of Rabanales, 14071 Cordoba, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Ríos-Moreno
authors.affiliation: Department of Agricultural and Forestry Sciences, ETSIAM, University of Cordoba, C4 Building, Campus of Rabanales, 14071 Cordoba, Spain.
authors.forename: I
authors.initials: I
authors.lastname: Garrido-Jurado
authors.affiliation: Department of Analytical Chemistry, University of Granada, Campus Fuentenueva s/n, 18071 Granada, Spain.
authors.forename: L
authors.initials: L
authors.lastname: Gámiz-Gracia
authors.affiliation: Department of Analytical Chemistry, University of Granada, Campus Fuentenueva s/n, 18071 Granada, Spain.
authors.forename: A M
authors.initials: AM
authors.lastname: García-Campaña
authors.affiliation: Department of Agricultural and Forestry Sciences, ETSIAM, University of Cordoba, C4 Building, Campus of Rabanales, 14071 Cordoba, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Quesada-Moraga
authors.affiliation: Department of Analytical Chemistry, University of Cordoba, Annex C3 Building, Campus of Rabanales, 14071 Cordoba, Spain. Electronic address: lourdes.arce@uco.es.
authors.forename: L
authors.initials: L
authors.lastname: Arce
mesh_terms: D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D047630:Depsipeptides / Q000302:isolation & purification*; D052981:Metarhizium / Q000502:physiology; D015203:Reproducibility of Results; D011198:Solanum tuberosum / Q000737:chemistry* / Q000382:microbiology; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013997:Time Factors
chemical_list: D047630:Depsipeptides
keywords: Destruxins; Potato plant; QuEChERS; UHPLC–MS/MS