title: Control of Attachment ofPseudomonas aeruginosaandBurkholderia cepaciato Surfaces by Shear Force.
abstract: The effect of physical shearing on the attachment of six Pseudomonas aeruginosa strains and six Burkholderia cepacia strains to glass, stainless steel, polystyrene and Teflon® was determined. A significant (p < 0.05) decrease in hydrophobicity was apparent for all P. aeruginosa strains (17-36%) and B. cepacia, MS 5 (20%) after shearing. A significant (p < 0.05) decrease in attachment of some P. aeruginosa (0.2-0.5 log CFU/cm2) and B. cepacia (0.2-0.4 log CFU/cm2) strains to some surface types was apparent after shearing. Significant (p < 0.05) correlation was observed for both numbers of flagellated cells and hydrophobicity against attachment to glass, stainless steel and polystyrene for P. aeruginosa while only hydrophobicity showed significant correlation against the same surfaces for B. cepacia. Scanning electron microscopy and protein analysis showed that shearing removed surface proteins from the cells and may have led to the observed changes in hydrophobicity and attachment to abiotic surfaces.
authors.affiliation: School of Science, Monash University, Jalan Lagoon Selatan, 46150 Bandar Sunway, Selangor, Malaysia.
authors.forename: Yew Woh
authors.initials: YW
authors.lastname: Hui
authors.forename: Kumaran
authors.initials: K
authors.lastname: Narayanan
authors.forename: Gary A
authors.initials: GA
authors.lastname: Dykes
mesh_terms: D001422:Bacterial Adhesion / Q000502:physiology*; D001426:Bacterial Proteins / Q000378:metabolism; D016956:Burkholderia cepacia / Q000502:physiology*; D005407:Flagella; D015964:Gene Expression Regulation, Bacterial; D057927:Hydrophobic and Hydrophilic Interactions; D009068:Movement; D011550:Pseudomonas aeruginosa / Q000502:physiology*; D033081:Shear Strength; D013499:Surface Properties; D014871:Water Microbiology
chemical_list: D001426:Bacterial Proteins