title: Sphingomonas qilianensis sp. nov., Isolated from Surface Soil in the Permafrost Region of Qilian Mountains, China.
abstract: A Gram-stain-negative, strictly aerobic, non-motile and rod-shaped bacterial strain, designated X1(T), was isolated from the permafrost region of Qilian Mountains in northwest of China. Phylogenetic analyses of 16S rRNA gene sequence revealed that strain X1(T) was a member of the genus Sphingomonas and shared the highest 16S rRNA gene sequence similarity with Sphingomonas oligophenolica JCM 12082(T) (96.9%), followed by Sphingomonas glacialis CGMCC 1.8957(T) (96.7%) and Sphingomonas alpina DSM 22537(T) (96.4%). Strain X1(T) was able to grow at 15-30 °C, pH 6.0-10.0 and with 0-0.3% NaCl (w/v). The DNA G+C content of the isolate was 64.8 mol%. Strain X1(T)-contained Q-10 as the dominant ubiquinone and C(18:1)ω7c, C(16:1)ω7c, C(16:0) and C(14:0) 2-OH as the dominant fatty acids. The polar lipid profile of strain XI(T)-contained sphingoglycolipid, phosphatidylglycerol, phosphatidylethanolamine, one unidentified glycolipid and two unidentified phospholipid. Due to the phenotypic and genetic distinctiveness and other characteristic studied in this article, we consider X1(T) as a novel species of the genus Sphingomonas and propose to name it Sphingomonas qilianensis sp. nov. The type strain is X1(T) (=CGMCC 1.15349(T) = KCTC 42862(T)).
authors.affiliation: College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, People's Republic of China.
authors.forename: Ai-Lian
authors.initials: AL
authors.lastname: Piao
authors.affiliation: College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, People's Republic of China.
authors.forename: Xiao-Min
authors.initials: XM
authors.lastname: Feng
authors.affiliation: Research and Development Center for Marine Biosciences, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 2-15 Natsushima-cho, Yokosuka, 237-0061, Japan.
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Nogi
authors.affiliation: College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, People's Republic of China.
authors.forename: Lu
authors.initials: L
authors.lastname: Han
authors.affiliation: Qinghai No.105 Coal Geological Exploration Team, Xining, 810007, Qinghai, China.
authors.forename: Yonghong
authors.initials: Y
authors.lastname: Li
authors.affiliation: College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, People's Republic of China. lvjie@mail.buct.edu.cn.
authors.forename: Jie
authors.initials: J
authors.lastname: Lv
mesh_terms: D001482:Base Composition; D002681:China; D004777:Environment; D065946:Permafrost / Q000382:microbiology*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D020578:Sphingomonas / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D012336:RNA, Ribosomal, 16S