title: Effects of Ethanol on the Expression Level of Various BDNF mRNA Isoforms and Their Encoded Protein in the Hippocampus of Adult and Embryonic Rats.
abstract: We aimed to compare the effects of oral ethanol (Eth) alone or combined with the phytoestrogen resveratrol (Rsv) on the expression of various brain-derived neurotrophic factor (BDNF) transcripts and the encoded protein pro-BDNF in the hippocampus of pregnant and embryonic rats. A low (0.25 g/kg body weight (BW)/day) dose of Eth produced an increase in the expression of BDNF exons I, III and IV and a decrease in that of the exon IX in embryos, but failed to affect BDNF transcript and pro-BDNF protein expression in adults. However, co-administration of Eth 0.25 g/kg·BW/day and Rsv led to increased expression of BDNF exons I, III and IV and to a small but significant increase in the level of pro-BDNF protein in maternal rats. A high (2.5 g/kg·BW/day) dose of Eth increased the expression of BDNF exons III and IV in embryos, but it decreased the expression of exon IX containing BDNF mRNAs in the maternal rats. While the high dose of Eth alone reduced the level of pro-BDNF in adults, it failed to change the levels of pro-BDNF in embryos. Eth differentially affects the expression pattern of BDNF transcripts and levels of pro-BDNF in the hippocampus of both adult and embryonic rats.
authors.affiliation: Department of Biochemistry and Recombinant Protein Laboratory, School of Medicine, Shiraz University of Medical Sciences, Shiraz 713484579, Iran. shahla_shojaei@yahoo.com.
authors.forename: Shahla
authors.initials: S
authors.lastname: Shojaei
authors.affiliation: Department of Human Anatomy and Cell Science, Faculty of Health Sciences College of Medicine, University of Manitoba, Winnipeg, MB R3E 0J9, Canada. saeid.ghavami@umanitoba.ca.|Health Research Policy Centre, Shiraz University of Medical Sciences, Shiraz 713484579, Iran. saeid.ghavami@umanitoba.ca.
authors.forename: Saeid
authors.initials: S
authors.lastname: Ghavami
authors.affiliation: Department of Pharmacology, School of Medicine, Shiraz University of Medical Sciences, Shiraz 713484579, Iran. panjeshm@sums.ac.ir.
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Panjehshahin
authors.affiliation: Research Center for Psychiatry and Behavioral Sciences, Shiraz University of Medical Sciences, Shiraz 713484579, Iran. owjiaa@sums.ac.ir.
authors.forename: Ali Akbar
authors.initials: AA
authors.lastname: Owji
mesh_terms: D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000235:genetics / Q000378:metabolism*; D000431:Ethanol / Q000494:pharmacology*; D005091:Exons; D005260:Female; D006624:Hippocampus / Q000187:drug effects* / Q000196:embryology / Q000378:metabolism; D011247:Pregnancy; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D020033:Protein Isoforms; D012333:RNA, Messenger; D013267:Stilbenes; D000431:Ethanol; D000077185:Resveratrol
keywords: alcohol; fetus; growth factor; neuroprotection; neurotoxicity; resveratrol; transcript