title: High-throughput deep screening and identification of four peripheral leucocyte microRNAs as novel potential combination biomarkers for preeclampsia.
abstract: OBJECTIVE
  To identify the specific microRNA (miRNA) biomarkers of preeclampsia (PE), the miRNA profiles analysis were performed.
  
  
  METHODS
  The blood samples were obtained from five PE patients and five normal healthy pregnant women. The small RNA profiles were analyzed to identify miRNA expression levels and find out miRNAs that may associate with PE. The quantitative reverse transcriptase-PCR (qRT-PCR) assay was used to validate differentially expressed peripheral leucocyte miRNAs in a new cohort.
  
  
  RESULTS
  The data analysis showed that 10 peripheral leucocyte miRNAs were significantly differently expressed in severe PE patients. Four differently expressed miRNAs were successfully validated using qRT-PCR method.
  
  
  CONCLUSIONS
  We successfully constructed a model with high accuracy to predict PE. A combination of four peripheral leucocyte miRNAs has great potential to serve as diagnostic biomarkers of PE.
authors.affiliation: The Second Hospital of Shanxi Medical University, TaiYuan, Shanxi Province, China.
authors.forename: Yonghong
authors.identifier: http://orcid.org/0000-0002-1683-6721
authors.initials: Y
authors.lastname: Wang
authors.affiliation: BGI-Shenzhen, Shenzhen, China.
authors.forename: Xukui
authors.initials: X
authors.lastname: Yang
authors.affiliation: The Second Hospital of Shanxi Medical University, TaiYuan, Shanxi Province, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Yang
authors.affiliation: The Second Hospital of Shanxi Medical University, TaiYuan, Shanxi Province, China.
authors.forename: Wenjun
authors.initials: W
authors.lastname: Wang
authors.affiliation: The Second Hospital of Shanxi Medical University, TaiYuan, Shanxi Province, China.
authors.forename: Meiling
authors.initials: M
authors.lastname: Zhao
authors.affiliation: The Second Hospital of Shanxi Medical University, TaiYuan, Shanxi Province, China.
authors.forename: Huiqiang
authors.initials: H
authors.lastname: Liu
authors.affiliation: The Second Hospital of Shanxi Medical University, TaiYuan, Shanxi Province, China.
authors.forename: Dongyan
authors.initials: D
authors.lastname: Li
authors.affiliation: The Second Hospital of Shanxi Medical University, TaiYuan, Shanxi Province, China.
authors.forename: Min
authors.initials: M
authors.lastname: Hao
mesh_terms: D015415:Biomarkers / Q000097:blood*; D016022:Case-Control Studies; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D007962:Leukocytes / Q000737:chemistry*; D035683:MicroRNAs / Q000097:blood*; D011225:Pre-Eclampsia / Q000097:blood / Q000175:diagnosis* / Q000235:genetics; D011247:Pregnancy; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017423:Sequence Analysis, RNA
chemical_list: D015415:Biomarkers; D035683:MicroRNAs