title: (+)-Catechin inhibition of transmissible gastroenteritis coronavirus in swine testicular cells is involved its antioxidation.
abstract: Transmissible gastroenteritis virus (TGEV) causes transmissible gastroenteritis (TGE), especially in newborn piglets, which severely threatens the worldwide pig industry. In this study, (+)-catechin was evaluated for its antiviral effect against TGEV in vitro. Viability assays revealed that (+)-catechin treatment exerted a dose-dependent rescue effect in TGEV-infected ST cells, and this result was only obtained with the post-treatment application of (+)-catechin. The viral yields in (+)-catechin-treated cultures were reduced by almost three log10 units. Quantitative real-time PCR analysis of the TGEV genome revealed that TGEV RNA replication was restricted after (+)-catechin treatment. Intracellular reactive oxygen species (ROS) detection showed that (+)-catechin alleviated ROS conditions induced by TGEV infection. Our results showed that (+)-catechin exerts an inhibitory effect on TGEV proliferation in vitro and is involved its antioxidation.
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
authors.forename: Wulong
authors.initials: W
authors.lastname: Liang
authors.affiliation: Animal Disease and Public Security Academician Workstation of Henan Province, The Key Lab of Animal Disease and Public Security, Henan University of Science and Technology, Luoyang 471003, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: He
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
authors.forename: Pengbo
authors.initials: P
authors.lastname: Ning
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
authors.forename: Jihui
authors.initials: J
authors.lastname: Lin
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
authors.forename: Helin
authors.initials: H
authors.lastname: Li
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Lin
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
authors.forename: Kai
authors.initials: K
authors.lastname: Kang
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, PR China. Electronic address: zhangym@nwsuaf.edu.cn.
authors.forename: Yanming
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D002392:Catechin / Q000494:pharmacology*; D002460:Cell Line; D005761:Gastroenteritis, Transmissible, of Swine / Q000188:drug therapy* / Q000821:virology; D008297:Male; D013552:Swine; D013553:Swine Diseases / Q000188:drug therapy* / Q000821:virology; D013737:Testis; D005760:Transmissible gastroenteritis virus / Q000502:physiology*; D014779:Virus Replication / Q000187:drug effects
chemical_list: D000998:Antiviral Agents; D002392:Catechin
keywords: (+)-Catechin; Antioxidation; Antiviral; Transmissible gastroenteritis coronavirus (TGEV)