title: Novel PCRs for differential diagnosis of cestodes.
abstract: Cestodes or tapeworms belong to a diverse group of helminths. The adult Taenia saginata and Taenia solium tapeworm can infest the human gut and the larval stage of Echinococcus spp. and T. solium can infect tissues of the human body, causing serious disease. Molecular diagnostics can be performed on proglottids, eggs and on cyst fluids taken by biopsy. Detection of cestodes when a helminthic infection is suspected is of vital importance and species determination is required for appropriate patient care. For routine diagnostics a single test that is able to detect and type a range of cestodes is preferable. We sought to improve our diagnostic procedure that used to rely on PCR and subsequent sequencing of the Cox1 and Nad1 genes. We have compared these PCRs with novel PCRs on the 12S rRNA and Nad5 gene and established the sensitivity and specificity. A single PCR on the 12S gene proved to be very suitable for detection and specification of Taenia sp. and Echinococcus sp. Both targets harbour enough polymorphic sites to determine the various Echinococcus species. The 12S PCR was most sensitive of all tested.
authors.affiliation: National Institute of Public Health and the Environment, Centre for Infectious Disease Control, Netherlands. Electronic address: jeroen.roelfsema@rivm.nl.
authors.forename: Jeroen H
authors.initials: JH
authors.lastname: Roelfsema
authors.affiliation: National Institute of Public Health and the Environment, Centre for Infectious Disease Control, Netherlands. Electronic address: nahid.nozari@rivm.nl.
authors.forename: Nahid
authors.initials: N
authors.lastname: Nozari
authors.affiliation: National Institute of Public Health and the Environment, Centre for Infectious Disease Control, Netherlands. Electronic address: elena.pinelli.ortiz@rivm.nl.
authors.forename: Elena
authors.initials: E
authors.lastname: Pinelli
authors.affiliation: National Institute of Public Health and the Environment, Centre for Infectious Disease Control, Netherlands. Electronic address: l.m.kortbeek@rivm.nl.
authors.forename: Laetitia M
authors.initials: LM
authors.lastname: Kortbeek
mesh_terms: D000818:Animals; D002590:Cestode Infections / Q000175:diagnosis*; D051545:Cyclooxygenase 1 / Q000235:genetics; D018509:DNA, Helminth / Q000737:chemistry / Q000302:isolation & purification; D003937:Diagnosis, Differential; D004443:Echinococcosis / Q000175:diagnosis; D004446:Echinococcus / Q000145:classification* / Q000235:genetics; D042967:Electron Transport Complex I / Q000235:genetics*; D005819:Genetic Markers; D006801:Humans; D009243:NAD / Q000235:genetics; D016133:Polymerase Chain Reaction; D012335:RNA, Ribosomal / Q000235:genetics*; D012680:Sensitivity and Specificity; D013621:Taenia / Q000145:classification* / Q000235:genetics; D013622:Taeniasis / Q000175:diagnosis
chemical_list: D018509:DNA, Helminth; D005819:Genetic Markers; D012335:RNA, Ribosomal; C075526:RNA, ribosomal, 12S; D009243:NAD; D051545:Cyclooxygenase 1; D042967:Electron Transport Complex I
keywords: 12S; Cestodes; Echinococcus; Nad5; PCR; Taenia; Typing