title: Modified TB rapid test by proteinase K for rapid diagnosis of pleural tuberculosis.
abstract: The diagnosis of pleural tuberculosis continues to be a challenge due to the low sensitivity of traditional diagnostic methods. Better and more rapid tests are needed for diagnosis of pleural TB. In this study, pleural fluids were tested with rapid test to determine Mycobacterium tuberculosis (MTB antigen). Affinity chromatography was used to purify specific polyclonal antibodies against MTB antigen. Pleural samples after decontamination were treated with proteinase K. Rapid test for pleural fluids was prepared by specific antibody. Rapid test was performed on 85 pleural fluid patients. The patients had a mean age of 46.55 ± 15.96 years and 38 were men. The performance of rapid test, using proteinase K, was found to be the most impressive: sensitivity 93%, specificity 94%, PPV 90%, and NPV 96% compared with adenosine deaminase test (ADA), PCR, smear, and culture. The present study did demonstrate that modified TB rapid test can substantially improve the diagnosis of extrapulmonary TB.
authors.affiliation: Tuberculosis Department, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Shamsi
authors.initials: S
authors.lastname: Yari
authors.affiliation: Tuberculosis Department, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Alireza
authors.initials: A
authors.lastname: Hadizadeh Tasbiti
authors.affiliation: Tuberculosis Department, Pasteur Institute of Iran, Tehran, Iran.|Chemical Injury Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
authors.forename: Mostafa
authors.initials: M
authors.lastname: Ghanei
authors.affiliation: National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Mohammad Ali
authors.initials: MA
authors.lastname: Shokrgozar
authors.affiliation: Tuberculosis Department, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Abolfazl
authors.initials: A
authors.lastname: Fateh
authors.affiliation: Blood transfusion research center, High Institute for research and education in transfusion medicine, Tehran, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Yari
authors.affiliation: Tuberculosis Department, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Ahmadreza
authors.initials: A
authors.lastname: Bahrmand
mesh_terms: D000243:Adenosine Deaminase / Q000378:metabolism; D000328:Adult; D000368:Aged; D000942:Antigens, Bacterial / Q000378:metabolism; D019286:Endopeptidase K / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification / Q000378:metabolism; D010994:Pleura / Q000382:microbiology; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D014396:Tuberculosis, Pleural / Q000175:diagnosis*; D055815:Young Adult
chemical_list: D000942:Antigens, Bacterial; D019286:Endopeptidase K; D000243:Adenosine Deaminase
keywords: TB rapid test; adenosine deaminase test; pleural tuberculosis; proteinase K