title: Microsatellites for Carpotroche brasiliensis (Flacourtiaceae), a useful species for agroforestry and ecosystem conservation.
abstract: OBJECTIVE
  We developed microsatellite markers for Carpotroche brasiliensis (Flacourtiaceae), a dioecious tree that is used as a food resource by midsize animals of the Brazilian fauna.
  
  
  RESULTS
  We designed 30 primer pairs using next-generation sequencing and classified 25 pairs as polymorphic. Observed heterozygosity ranged from 0.5 to 1.0, and expected heterozygosity ranged from 0.418 to 0.907. The combined probability of exclusion was greater than 0.999 and the combined probability of identity was less than 0.001, indicating that these microsatellites are appropriate for investigations of genetic structure, individual identification, and paternity testing.
  
  
  CONCLUSIONS
  The developed molecular tools may contribute to future studies of population genetics, answering ecological and evolutionary questions regarding efficient conservation strategies for C. brasiliensis.
authors.affiliation: Laboratório de Marcadores Moleculares, Centro de Biotecnologia e Genética (CBG), Universidade Estadual de Santa Cruz (UESC), Campus Soane Nazaré de Andrade, Rodovia Jorge Amado, Km 16, Bairro Salobrinho, 45662-900 Ilhéus, Bahia, Brazil.
authors.forename: Flora
authors.initials: F
authors.lastname: Bittencourt
authors.affiliation: Universidade Estadual do Sudoeste da Bahia (UESB), Rodovia BR 415, Km 03 s/n, 45700-000 Itapetinga, Bahia, Brazil.
authors.forename: Jackeline S
authors.initials: JS
authors.lastname: Alves
authors.affiliation: Laboratório de Marcadores Moleculares, Centro de Biotecnologia e Genética (CBG), Universidade Estadual de Santa Cruz (UESC), Campus Soane Nazaré de Andrade, Rodovia Jorge Amado, Km 16, Bairro Salobrinho, 45662-900 Ilhéus, Bahia, Brazil.
authors.forename: Fernanda A
authors.initials: FA
authors.lastname: Gaiotto
keywords: Carpotroche brasiliensis; Flacourtiaceae; endemic species; next-generation sequencing; simple sequence repeat (SSR); tropical forest