title: miRNA-592 is downregulated and may target LHCGR in polycystic ovary syndrome patients.
abstract: The polycystic ovary syndrome (PCOS) is an endocrine disorder mainly associated with infertility. Abnormal regulation of relevant genes is required for follicular development in PCOS. In the current study, the expression of serum miRNAs of PCOS patients was explored using miRNA array followed by qRT-PCR assays. The circulating level of miR-592 was significantly down-regulated in PCOS patients in comparison with healthy controls. Furthermore, we found that miR-592 was inversely correlated with the level of luteinizing hormone/chorionic gonadotropin receptor (LHCGR). Computational analysis predicted that miR-592 interacts with the LHCGR mRNA via binding to a site located in the 3'UTR region. Using a luciferase-based reporter assay we found that miR-592 directly targeted the LHCGR. In KGN cell line, miR-592 overexpression inhibited cell viability and the transition of phase G1 to phase S. Knocking down of LHCGR inhibited cell viability and cell cycle progression in KGN cells, and LHCGR co-transfection reversed the inhibitory effect of miR-592. These results shed new light on the diagnosis and treatment of PCOS syndrome.
authors.affiliation: Center of Reproductive Medicine, Department of Obstetrics and Gynecology, West China Second University Hospital of Sichuan University, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Song
authors.affiliation: Center of Reproductive Medicine, Department of Obstetrics and Gynecology, West China Second University Hospital of Sichuan University, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Luo
authors.affiliation: Center of Reproductive Medicine, Department of Obstetrics and Gynecology, West China Second University Hospital of Sichuan University, China. Electronic address: lishangwei0117@126.com.
authors.forename: Shang-Wei
authors.initials: SW
authors.lastname: Li
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D002453:Cell Cycle; D002460:Cell Line; D002470:Cell Survival; D015536:Down-Regulation / Q000502:physiology*; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology*; D055785:Gene Knockdown Techniques; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D011085:Polycystic Ovary Syndrome / Q000235:genetics* / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011974:Receptors, LH / Q000235:genetics / Q000378:metabolism*
chemical_list: C000588991:MIRN592 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; D011974:Receptors, LH
keywords: LH; LHCGR; MiR-592; MiRNA; PCOS