title: Distribution in Different Organisms of Amino Acid Oxidases with FAD or a Quinone As Cofactor and Their Role as Antimicrobial Proteins in Marine Bacteria.
abstract: Amino acid oxidases (AAOs) catalyze the oxidative deamination of amino acids releasing ammonium and hydrogen peroxide. Several kinds of these enzymes have been reported. Depending on the amino acid isomer used as a substrate, it is possible to differentiate between l-amino acid oxidases and d-amino acid oxidases. Both use FAD as cofactor and oxidize the amino acid in the alpha position releasing the corresponding keto acid. Recently, a novel class of AAOs has been described that does not contain FAD as cofactor, but a quinone generated by post-translational modification of residues in the same protein. These proteins are named as LodA-like proteins, after the first member of this group described, LodA, a lysine epsilon oxidase synthesized by the marine bacterium Marinomonas mediterranea. In this review, a phylogenetic analysis of all the enzymes described with AAO activity has been performed. It is shown that it is possible to recognize different groups of these enzymes and those containing the quinone cofactor are clearly differentiated. In marine bacteria, particularly in the genus Pseudoalteromonas, most of the proteins described as antimicrobial because of their capacity to generate hydrogen peroxide belong to the group of LodA-like proteins.
authors.affiliation: Department of Genetics and Microbiology, Faculty of Biology, University of Murcia, Murcia 30100, Spain. jonatancristian.campillo@um.es.
authors.forename: Jonatan C
authors.initials: JC
authors.lastname: Campillo-Brocal
authors.affiliation: Department of Genetics and Microbiology, Faculty of Biology, University of Murcia, Murcia 30100, Spain. patlucel@um.es.
authors.forename: Patricia
authors.initials: P
authors.lastname: Lucas-Elío
authors.affiliation: Department of Genetics and Microbiology, Faculty of Biology, University of Murcia, Murcia 30100, Spain. antonio@um.es.
authors.forename: Antonio
authors.initials: A
authors.lastname: Sanchez-Amat
mesh_terms: D000594:Amino Acid Oxidoreductases / Q000378:metabolism; D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D000890:Anti-Infective Agents / Q000302:isolation & purification / Q000494:pharmacology*; D059001:Aquatic Organisms / Q000382:microbiology; D001419:Bacteria / Q000302:isolation & purification*; D001426:Bacterial Proteins / Q000378:metabolism; D005182:Flavin-Adenine Dinucleotide / Q000378:metabolism; D006801:Humans; D011809:Quinones / Q000378:metabolism
chemical_list: D000596:Amino Acids; D000890:Anti-Infective Agents; D001426:Bacterial Proteins; D011809:Quinones; D005182:Flavin-Adenine Dinucleotide; D000594:Amino Acid Oxidoreductases
keywords: amino acid oxidases; antimicrobial; flavoprotein; quinone cofactor