title: Effect of rainbow trout (Oncorhynchus mykiss) seminal plasma on the post-thaw quality of ram semen cryopreserved in a soybean lecithin-based or egg yolk-based extender.
abstract: The aim of the current study was to evaluate the effects of different concentrations of rainbow trout seminal plasma (RTSP) (0.1%, 1% and 10%) in extenders containing either egg yolk or lecithin for use in Awassi ram semen cryopreservation. Pooled sperm were diluted in a two-step dilution method to a final concentration of 1/5 (semen/extender) in egg yolk or lecithin extender containing no RTSP, 0.1%, 1% or 10% RTSP (v/v). Semen samples were assessed for sperm motility, plasma membrane integrity [hypoosmotic swelling test (HOST) and Hoechst 33258] and defective acrosomes [FITC-conjugated Pisum sativum agglutinin (PSA-FITC)] at the following five time points: after dilution with extender A; after equilibration; and post-thaw at 0h, 3h and 5h. Malondialdehyde (MDA) was examined only after thawing. Freezing and thawing procedures (dilution, equilibration and post-thaw incubation at 0h, 3h and 5h) negatively affected the motility (P<0.001) and acrosome integrity (P<0.001). Additionally, freezing and thawing negatively affected the plasma membrane integrity, as determined by the HOST and Hoechst 33258 (P<0.001). The extender group affected the motility (P<0.001) and the HOST results (P<0.001). Levels of MDA in the egg yolk extender with 1% RTSP group were significantly lower than in the lecithin control group (P<0.05). In conclusion, the egg yolk extender groups that were supplemented with 10% and 1% RTSP provided greater cryoprotective effects for semen survivability during 5h incubation than the other extender groups.
authors.affiliation: Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Uludag University, Gorukle, Bursa 16059, Turkey. Electronic address: bbaspinar@uludag.edu.tr.
authors.forename: Burcu
authors.initials: B
authors.lastname: Ustuner
authors.affiliation: Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Uludag University, Gorukle, Bursa 16059, Turkey.
authors.forename: Selim
authors.initials: S
authors.lastname: Alcay
authors.affiliation: Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Uludag University, Gorukle, Bursa 16059, Turkey.
authors.forename: M Berk
authors.initials: MB
authors.lastname: Toker
authors.affiliation: Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Uludag University, Gorukle, Bursa 16059, Turkey.
authors.forename: Zekariya
authors.initials: Z
authors.lastname: Nur
authors.affiliation: Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Uludag University, Gorukle, Bursa 16059, Turkey.
authors.forename: Elif
authors.initials: E
authors.lastname: Gokce
authors.affiliation: Department of Physiology, Faculty of Veterinary Medicine, Uludag University, Gorukle, Bursa 16059, Turkey.
authors.forename: Fusun Ak
authors.initials: FA
authors.lastname: Sonat
authors.affiliation: Department of Pharmacology, Faculty of Medicine, Uludag University, Gorukle, Bursa 16059, Turkey.
authors.forename: Zulfiye
authors.initials: Z
authors.lastname: Gul
authors.affiliation: Department of Aquatic Animal Disease, Faculty of Veterinary Medicine, Uludag University, Gorukle, Bursa 16059, Turkey.
authors.forename: Muhammed
authors.initials: M
authors.lastname: Duman
authors.affiliation: Faculty of Veterinary Medicine, Uludag University, Gorukle, Bursa 16059, Turkey.
authors.forename: Cafer
authors.initials: C
authors.lastname: Ceniz
authors.affiliation: Faculty of Veterinary Medicine, Uludag University, Gorukle, Bursa 16059, Turkey.
authors.forename: Aydın
authors.initials: A
authors.lastname: Uslu
authors.affiliation: Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Uludag University, Gorukle, Bursa 16059, Turkey.
authors.forename: Hakan
authors.initials: H
authors.lastname: Sagirkaya
authors.affiliation: Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Uludag University, Gorukle, Bursa 16059, Turkey.
authors.forename: M Kemal
authors.initials: MK
authors.lastname: Soylu
mesh_terms: D000177:Acrosome; D000818:Animals; D002462:Cell Membrane; D015925:Cryopreservation / Q000662:veterinary*; D003451:Cryoprotective Agents; D004530:Egg Yolk; D008297:Male; D008315:Malondialdehyde; D017686:Oncorhynchus mykiss; D037121:Plant Lectins; D012661:Semen / Q000502:physiology*; D012662:Semen Preservation / Q000662:veterinary; D012756:Sheep; D030262:Soybean Proteins; D010835:Phytohemagglutinins
chemical_list: D003451:Cryoprotective Agents; D008315:Malondialdehyde; D037121:Plant Lectins; D030262:Soybean Proteins; C028824:soybean lectin; D010835:Phytohemagglutinins
keywords: Cryopreservation; Rainbow trout; Ram semen; Seminal plasma