title: Purification of glucose-6-phosphate dehydrogenase and glutathione reductase enzymes from the gill tissue of Lake Van fish and analyzing the effects of some chalcone derivatives on enzyme activities.
abstract: Glucose-6-phosphate dehydrogenase (G6PD) and glutathione reductase (GR) are metabolically quite important enzymes. Within this study, these two enzymes were purified for the first time from the gills of Lake Van fish. In the purifying process, ammonium sulfate precipitation and 2',5'-ADP Sepharose 4B affinity column chromatography techniques for glucose-6-phosphate dehydrogenase, temperature degradation and 2',5'-ADP Sepharose 4B affinity column chromatography for glutathione reductase enzyme were used. The control of the enzyme purity and determination of molecular weight were done with sodium dodecyl sulfate polyacrylamide gel electrophoresis. K(M) and V(max) values were determined with Lineweaver-Burk plot. Besides, the effects of some chalcone derivatives on the purified enzymes were analyzed. For the ones showing inhibition effect, % activity-[I] figures were drawn and IC50 values were determined. K(i) value was calculated by using Cheng-Prusoff equation.
authors.affiliation: Faculty of Pharmacy, University of Ağrı İbrahim Çeçen, 04100, Ağrı, Turkey. mkuzu@agri.edu.tr.
authors.forename: Muslum
authors.initials: M
authors.lastname: Kuzu
authors.affiliation: Department of Nutrition and Dietetics, University of Ağrı İbrahim Çeçen, Ağrı, Turkey.
authors.forename: Abdulselam
authors.initials: A
authors.lastname: Aslan
authors.affiliation: Karlsruhe Institute of Technology, Karlsruhe, Germany.
authors.forename: Ishtiaq
authors.initials: I
authors.lastname: Ahmed
authors.affiliation: Department of Nutrition and Dietetics, University of Ağrı İbrahim Çeçen, Ağrı, Turkey.
authors.forename: Veysel
authors.initials: V
authors.lastname: Comakli
authors.affiliation: Department of Nutrition and Dietetics, University of Ağrı İbrahim Çeçen, Ağrı, Turkey.
authors.forename: Ramazan
authors.initials: R
authors.lastname: Demirdag
authors.affiliation: Faculty of Pharmacy, University of Ağrı İbrahim Çeçen, 04100, Ağrı, Turkey.
authors.forename: Naim
authors.initials: N
authors.lastname: Uzun
mesh_terms: D000818:Animals; D002599:Chalcone / Q000633:toxicity*; D005399:Fishes / Q000378:metabolism; D005880:Gills / Q000378:metabolism*; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism*; D060106:Lakes; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D014874:Water Pollutants, Chemical; D002599:Chalcone; D005954:Glucosephosphate Dehydrogenase; D005980:Glutathione Reductase
keywords: Chalcone; Glucose-6-phosphate dehydrogenase; Glutathione reductase; Inhibition; Lake Van fish