title: Regenerated bacterial cellulose microfluidic column for glycoproteins separation.
abstract: To analysis and separate glycoproteins, a simple strategy to prepare regenerated bacterial cellulose (RBC) column with concanavalin A (Con A) lectin immobilized in microfluidic system was applied. RBC was filled into microchannel to fabricate RBC microcolumn after bacterial cellulose dissolved in NaOH-sulfourea water solution. Lectin Con A was covalently connected onto RBC matrix surface via Schiff-base formation. Lysozyme (non-glycoprotein) and transferrin (glycoprotein) were successfully separated based on their different affinities toward the immobilized Con A. Overall, the RBC microfluidic system presents great potential application in affinity chromatography of glycoproteins analysis, and this research represents a significant step to prepare bacterial cellulose (BC) as column packing material in microfluidic system. What is more, troublesome operations for lectin affinity chromatography were simplified by integrating the microfluidic chip onto a HPLC (High Performance Liquid Chromatography) system.
authors.affiliation: Institute of Chemicobiology and Functional Materials, Nanjing University of Science and Technology, Xiao Ling Wei 200#, 210094 Nanjing, China; School of Chemical Engineering, Nanjing University of Science and Technology, Xiao Ling Wei 200#, 210094 Nanjing, China.
authors.forename: Chuntao
authors.initials: C
authors.lastname: Chen
authors.affiliation: Institute of Chemicobiology and Functional Materials, Nanjing University of Science and Technology, Xiao Ling Wei 200#, 210094 Nanjing, China; School of Chemical Engineering, Nanjing University of Science and Technology, Xiao Ling Wei 200#, 210094 Nanjing, China.
authors.forename: Chunlin
authors.initials: C
authors.lastname: Zhu
authors.affiliation: Institute of Chemicobiology and Functional Materials, Nanjing University of Science and Technology, Xiao Ling Wei 200#, 210094 Nanjing, China; School of Chemical Engineering, Nanjing University of Science and Technology, Xiao Ling Wei 200#, 210094 Nanjing, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Institute of Chemicobiology and Functional Materials, Nanjing University of Science and Technology, Xiao Ling Wei 200#, 210094 Nanjing, China; School of Chemical Engineering, Nanjing University of Science and Technology, Xiao Ling Wei 200#, 210094 Nanjing, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Nie
authors.affiliation: Institute of Chemicobiology and Functional Materials, Nanjing University of Science and Technology, Xiao Ling Wei 200#, 210094 Nanjing, China; School of Chemical Engineering, Nanjing University of Science and Technology, Xiao Ling Wei 200#, 210094 Nanjing, China.
authors.forename: Jiazhi
authors.initials: J
authors.lastname: Yang
authors.affiliation: School of Chemical Engineering, Nanjing University of Science and Technology, Xiao Ling Wei 200#, 210094 Nanjing, China.
authors.forename: Ruiqi
authors.initials: R
authors.lastname: Shen
authors.affiliation: Institute of Chemicobiology and Functional Materials, Nanjing University of Science and Technology, Xiao Ling Wei 200#, 210094 Nanjing, China; School of Chemical Engineering, Nanjing University of Science and Technology, Xiao Ling Wei 200#, 210094 Nanjing, China. Electronic address: sundpe301@163.com.
authors.forename: Dongping
authors.initials: D
authors.lastname: Sun
mesh_terms: D002482:Cellulose / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D003208:Concanavalin A / Q000737:chemistry; D006023:Glycoproteins / Q000737:chemistry*; D044085:Microfluidics / Q000379:methods*; D009113:Muramidase / Q000737:chemistry; D014168:Transferrin / Q000737:chemistry
chemical_list: D006023:Glycoproteins; D014168:Transferrin; D003208:Concanavalin A; D002482:Cellulose; D009113:Muramidase
keywords: Column packing; Glycoproteins separation; Microfluidic; Regenerated bacterial cellulose