title: Preparation, characterization and feasibility study of dialdehyde carboxymethyl cellulose as a novel crosslinking reagent.
abstract: The natural biopolymers usually need to be chemically modified by crosslinking reagents to improve their mechanical properties. In the present research, the feasibility of using the dialdehyde carboxymethyl cellulose (DCMC) as a crosslinking reagent was systematically studied. DCMC was prepared by oxidizing carboxymethyl cellulose using sodium periodate. The formation of dialdehyde groups was confirmed by FTIR and the degree of oxidation was determined. The biocompatibility of DCMC was investigated by evaluating its cytotoxicity to L929 fibroblasts and histocompatibility in rat model via intramuscular and subcutaneous injection. DCMC-crosslinked carboxymethyl chitosan (DCMC-CMCTS) was prepared and characterized using the glutaraldehyde-crosslinked CMCTS (GA-CMCTS) as control. The result demonstrated that DCMC was non-cytotoxic, biodegradable and biocompatible. The DCMC-CMCTS displayed significantly better thermostability, swelling capacity and cyto-compatibility compared with GA-CMCTS. Our data provided experimental basis for the future application of DCMC as a novel crosslinking reagent.
authors.affiliation: Biochemistry Laboratory, College of Marine Life Sciences, Ocean University of China, No. 5 Yushan Road, Qingdao 266003, Shandong, PR China.
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Jiang
authors.affiliation: Department of Anatomy, Physiology and Pharmacology, College of Veterinary Medicine, Auburn University, Auburn, AL 36849, United States.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Biochemistry Laboratory, College of Marine Life Sciences, Ocean University of China, No. 5 Yushan Road, Qingdao 266003, Shandong, PR China.
authors.forename: Yanfei
authors.initials: Y
authors.lastname: Peng
authors.affiliation: Biochemistry Laboratory, College of Marine Life Sciences, Ocean University of China, No. 5 Yushan Road, Qingdao 266003, Shandong, PR China. Electronic address: baoqinh@ouc.edu.cn.
authors.forename: Baoqin
authors.initials: B
authors.lastname: Han
authors.affiliation: Biochemistry Laboratory, College of Marine Life Sciences, Ocean University of China, No. 5 Yushan Road, Qingdao 266003, Shandong, PR China.
authors.forename: Zhuoyue
authors.initials: Z
authors.lastname: Li
authors.affiliation: Biochemistry Laboratory, College of Marine Life Sciences, Ocean University of China, No. 5 Yushan Road, Qingdao 266003, Shandong, PR China.
authors.forename: Xiuhua
authors.initials: X
authors.lastname: Li
authors.affiliation: Biochemistry Laboratory, College of Marine Life Sciences, Ocean University of China, No. 5 Yushan Road, Qingdao 266003, Shandong, PR China.
authors.forename: Wanshun
authors.initials: W
authors.lastname: Liu
mesh_terms: D000818:Animals; D002240:Carbohydrate Sequence; D002266:Carboxymethylcellulose Sodium / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D002482:Cellulose / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D003432:Cross-Linking Reagents / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D003603:Cytotoxins / Q000009:adverse effects / Q000737:chemistry; D005240:Feasibility Studies; D005347:Fibroblasts / Q000187:drug effects; D007249:Inflammation; D008297:Male; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D003432:Cross-Linking Reagents; D003603:Cytotoxins; D002482:Cellulose; C058684:2,3-dialdehydocellulose; D002266:Carboxymethylcellulose Sodium
keywords: Biocompatibility; Carboxymethyl chitosan; Crosslinking reagent; Dialdehyde carboxymethyl cellulose; Natural biopolymer