title: Two-Dimensional Crystallization of the Ca(2+)-ATPase for Electron Crystallography.
abstract: Electron crystallography of two-dimensional crystalline arrays is a powerful alternative for the structure determination of membrane proteins. The advantages offered by this technique include a native membrane environment and the ability to closely correlate function and dynamics with crystalline preparations and structural data. Herein, we provide a detailed protocol for the reconstitution and two-dimensional crystallization of the sarcoplasmic reticulum calcium pump (also known as Ca(2+)-ATPase or SERCA) and its regulatory subunits phospholamban and sarcolipin.
authors.affiliation: Department of Biochemistry, University of Alberta, Edmonton, AB, Canada, T6G 2H7.
authors.forename: John Paul
authors.initials: JP
authors.lastname: Glaves
authors.affiliation: Department of Biochemistry, University of Alberta, Edmonton, AB, Canada, T6G 2H7.
authors.forename: Joseph O
authors.initials: JO
authors.lastname: Primeau
authors.affiliation: Department of Biochemistry, University of Alberta, Edmonton, AB, Canada, T6G 2H7. hyoung@ualberta.ca.
authors.forename: Howard S
authors.initials: HS
authors.lastname: Young
mesh_terms: D002135:Calcium-Binding Proteins / Q000737:chemistry; D003460:Crystallization / Q000379:methods*; D008274:Magnesium / Q000737:chemistry; D008854:Microscopy, Electron; D009124:Muscle Proteins / Q000737:chemistry; D010712:Phosphatidic Acids / Q000737:chemistry; D011510:Proteolipids / Q000737:chemistry; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases / Q000737:chemistry*; D000099211:Phospholamban
chemical_list: D002135:Calcium-Binding Proteins; D008274:Magnesium; D009124:Muscle Proteins; D010712:Phosphatidic Acids; D011510:Proteolipids; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases; D000099211:Phospholamban; C077258:sarcolipin
keywords: Ca2+ -ATPase; Electron crystallography; Phospholamban; Sarcolipin; Sarcoplasmic reticulum; Two-dimensional crystals