title: Peanut proteins in periodate specific anion sensing: An ensuing reduction in allergic response.
abstract: Peanut proteins conarachin II, conarachin I and arachin were found to behave as highly selective fluorescence sensors for periodate amongst a set of different anions. The interactions of the proteins with periodate were also confirmed by other spectral methods and enzyme linked immunosorbent assay (ELISA). The results indicate a selective interaction of peanut proteins with periodate amongst chloride, sulphate, iodide, phosphate, nitrate, nitrite, bromide, fluoride, persulphate, acetate, thiosulphate, arsenite, arsenate, sulphite, and iodide. Periodate sensing using different synthesized organic molecules are already reported in the literature. In this article we report the efficiency of peanut proteins as anion sensor which are bioactive and inexpensive too. The protein periodate interactions have also resulted in a simultaneous reduction in allergenicity of the peanut proteins. A change in the secondary structure of the protein was found responsible for this change which was further established with the help of circular dichroism and Raman spectroscopy.
authors.affiliation: Department of Chemistry, University of Calcutta, 92, APC Road, Kolkata 700 009, India.
authors.forename: Sumanta Kumar
authors.initials: SK
authors.lastname: Ghatak
authors.affiliation: Mugneeram Ramkowar Bangur Hospital, 241-Desapran Sasmal Road, Kolkata 700 033, India.
authors.forename: Souvik
authors.initials: S
authors.lastname: Sen
authors.affiliation: Department of Physics, Bose Institute, 93/1, Acharya Prafulla Chandra Road, Kolkata 700009, India.
authors.forename: Dipanwita
authors.initials: D
authors.lastname: Majumdar
authors.affiliation: Department of Physics, Bose Institute, 93/1, Acharya Prafulla Chandra Road, Kolkata 700009, India.
authors.forename: Achintya
authors.initials: A
authors.lastname: Singha
authors.affiliation: Department of Chemistry, University of Calcutta, 92, APC Road, Kolkata 700 009, India. Electronic address: kamalchem.roy@gmail.com.
authors.forename: Kamalika
authors.initials: K
authors.lastname: Sen
mesh_terms: D000838:Anions / Q000737:chemistry; D010367:Arachis / Q000737:chemistry*; D002942:Circular Dichroism; D004797:Enzyme-Linked Immunosorbent Assay; D021183:Peanut Hypersensitivity / Q000517:prevention & control*; D010504:Periodic Acid / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry*; D017433:Protein Structure, Secondary; D013050:Spectrometry, Fluorescence; D013059:Spectrum Analysis, Raman
chemical_list: D000838:Anions; D010940:Plant Proteins; D010504:Periodic Acid; C009288:metaperiodate
keywords: Anion sensing; ELISA; Peanut proteins; Periodate; Spectroscopy