title: Evaluation of the binding interaction between bovine serum albumin and dimethyl fumarate, an anti-inflammatory drug by multispectroscopic methods.
abstract: The information of the quenching reaction of bovine serum albumin with dimethyl fumarate is obtained by multi-spectroscopic methods. The number of binding sites, n and binding constants, KA were determined at different temperatures. The effect of increasing temperature on Stern-Volmer quenching constants (KD) indicates that a dynamic quenching mechanism is involved in the interaction. The analysis of thermodynamic quantities namely, ∆H° and ∆S° suggested hydrophobic forces playing a major role in the interaction between dimethyl fumarate and bovine serum albumin. The binding site of dimethyl fumarate on bovine serum albumin was determined by displacement studies, using the site probes viz., warfarin, ibuprofen and digitoxin. The determination of magnitude of the distance of approach for molecular interactions between dimethyl fumarate and bovine serum albumin is calculated according to the theory of Förster energy transfer. The CD, 3D fluorescence spectra, synchronous fluorescence measurements and FT-IR spectral results were indicative of the change in secondary structure of the protein. The influence of some of the metal ions on the binding interaction was also studied.
authors.affiliation: P.G. Department of Studies in Chemistry, Karnatak University, Dharwad 580 003, India.
authors.forename: Laxmi
authors.initials: L
authors.lastname: Jattinagoudar
authors.affiliation: P.G. Department of Studies in Chemistry, Karnatak University, Dharwad 580 003, India.
authors.forename: Manjunath
authors.initials: M
authors.lastname: Meti
authors.affiliation: P.G. Department of Studies in Chemistry, Karnatak University, Dharwad 580 003, India.
authors.forename: Sharanappa
authors.initials: S
authors.lastname: Nandibewoor
authors.affiliation: P.G. Department of Studies in Chemistry, Karnatak University, Dharwad 580 003, India. Electronic address: schimatadar@gmail.com.
authors.forename: Shivamurti
authors.initials: S
authors.lastname: Chimatadar
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000378:metabolism*; D001665:Binding Sites; D002417:Cattle; D000069462:Dimethyl Fumarate / Q000378:metabolism*; D008958:Models, Molecular; D011485:Protein Binding; D017433:Protein Structure, Secondary / Q000187:drug effects; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D017550:Spectroscopy, Fourier Transform Infrared; D013816:Thermodynamics
chemical_list: D000893:Anti-Inflammatory Agents; D012710:Serum Albumin, Bovine; D000069462:Dimethyl Fumarate
keywords: Bovine serum albumin; Dimethyl fumarate; Dynamic quenching; Multispectroscopic techniques