title: Pretreatment with baicalin attenuates hypoxia and glucose deprivation-induced injury in SH-SY5Y cells.
abstract: OBJECTIVE
  To explore the neuroprotective effects of baicalin against hypoxia and glucose deprivation-reperfusion (OGD/RO)-induced injury in SH-SY5Y cells.
  
  
  METHODS
  SH-SY5Y cells were divided into a control group, a OGD/RO group, which was subject to OGD/RO induction; and 3 baicalin groups subject to baicalin (1, 5, 25 μmol/L) for 2 h before induction of OGD/RO (low-, medium-, and high-dose baicalin groups). Cell viability was detected by thiazolyl blue tetrazolium bromide (MTT) assay and flow cytometric analysis was used to detect cell apoptosis. Real-time polymerase chain reaction was performed to determine the mRNA expression of caspase-3 gene. Western blot analysis was conducted to determine the expression of nuclear factor (NF)-κB and N-methyl-daspartic acid receptor-1 (NMDAR1).
  
  
  RESULTS
  Baicalin could significantly attenuate OGD/RO mediated apoptotic cell death in SH-SY5Y cells; the apoptosis rates in the low-, medium- and high-dose groups were 12.1%, 7.9%, and 5.4%, respectively. Western blot and real-time PCR analysis revealed that significant decrease in caspase-3 expression in the baicalin group compared with the OGD/RO group (P<0.01). Additionally, down-regulation of NF-κB and NMDAR1 was observed in the baicalin group compared with those obtained from the OGD/RO group. Compared with the low-dose baicalin group, remarkable decrease was noted in the medium- and high-dose groups (P<0.01).
  
  
  CONCLUSIONS
  Baicalin pre-treatment attenuates brain ischemia reperfusion injury by suppressing cellular apoptosis.
authors.affiliation: Department of Neurology, the Second Hospital of Shandong University, Jinan, 250033, China.
authors.forename: Qing-bo
authors.initials: QB
authors.lastname: Zhou
authors.affiliation: Department of Neurology, the Second Hospital of Shandong University, Jinan, 250033, China.
authors.forename: Xiao-ning
authors.initials: XN
authors.lastname: Ju
authors.affiliation: Department of Neurology, the Second Hospital of Shandong University, Jinan, 250033, China.
authors.forename: Xiao-yun
authors.initials: XY
authors.lastname: Wang
authors.affiliation: Department of Neurology, the Second Hospital of Shandong University, Jinan, 250033, China.
authors.forename: Mei-hong
authors.initials: MH
authors.lastname: Wang
authors.affiliation: Central Laboratory, the Second Hospital of Shandong University, Jinan, 250033, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Kong
authors.affiliation: Central Laboratory, the Second Hospital of Shandong University, Jinan, 250033, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Sun
authors.affiliation: Department of Neurology, the Second Hospital of Shandong University, Jinan, 250033, China. lianxiang1210@163.com.
authors.forename: Jian-zhong
authors.initials: JZ
authors.lastname: Bi
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D016923:Cell Death / Q000187:drug effects; D015687:Cell Hypoxia / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D005419:Flavonoids / Q000494:pharmacology*; D005947:Glucose / Q000378:metabolism*; D006801:Humans; D016328:NF-kappa B / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism; D015424:Reperfusion
chemical_list: D005419:Flavonoids; C485668:GRIN1 protein, human; D016328:NF-kappa B; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D016194:Receptors, N-Methyl-D-Aspartate; C038044:baicalin; D053148:Caspase 3; D005947:Glucose
keywords: N-methyl-d-aspartic acid receptor-1; apoptosis; baicalin; caspase-3; hypoxia and glucose deptivation-reperfusion; neuroprotection; nuclear factor-κB