title: Systems Biology Approaches to Understand Natural Products Biosynthesis.
abstract: Actinomycetes populate soils and aquatic sediments that impose biotic and abiotic challenges for their survival. As a result, actinomycetes metabolism and genomes have evolved to produce an overwhelming diversity of specialized molecules. Polyketides, non-ribosomal peptides, post-translationally modified peptides, lactams, and terpenes are well-known bioactive natural products with enormous industrial potential. Accessing such biological diversity has proven difficult due to the complex regulation of cellular metabolism in actinomycetes and to the sparse knowledge of their physiology. The past decade, however, has seen the development of omics technologies that have significantly contributed to our better understanding of their biology. Key observations have contributed toward a shift in the exploitation of actinomycete's biology, such as using their full genomic potential, activating entire pathways through key metabolic elicitors and pathway engineering to improve biosynthesis. Here, we review recent efforts devoted to achieving enhanced discovery, activation, and manipulation of natural product biosynthetic pathways in model actinomycetes using genome-scale biological datasets.
authors.affiliation: Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland , Brisbane, QLD , Australia ; National Laboratory of Genomics for Biodiversity (LANGEBIO), Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (Cinvestav-IPN) , Irapuato , México.
authors.forename: Cuauhtemoc
authors.initials: C
authors.lastname: Licona-Cassani
authors.affiliation: National Laboratory of Genomics for Biodiversity (LANGEBIO), Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (Cinvestav-IPN) , Irapuato , México.
authors.forename: Pablo
authors.initials: P
authors.lastname: Cruz-Morales
authors.affiliation: Departamento de Biología Funcional and Instituto Universitario de Oncología del Principado de Asturias (IUOPA), Facultad de Medicina, Universidad de Oviedo , Oviedo , Spain.
authors.forename: Angel
authors.initials: A
authors.lastname: Manteca
authors.affiliation: National Laboratory of Genomics for Biodiversity (LANGEBIO), Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (Cinvestav-IPN) , Irapuato , México.
authors.forename: Francisco
authors.initials: F
authors.lastname: Barona-Gomez
authors.affiliation: Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland , Brisbane, QLD , Australia.
authors.forename: Lars K
authors.initials: LK
authors.lastname: Nielsen
authors.affiliation: Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland , Brisbane, QLD , Australia.
authors.forename: Esteban
authors.initials: E
authors.lastname: Marcellin
keywords: actinomycetes; genome mining; genome-scale metabolic reconstructions; genomics; metabolomics; proteomics; transcriptomics