title: Analysis of Molecular Species Profiles of Ceramide-1-phosphate and Sphingomyelin Using MALDI-TOF Mass Spectrometry.
abstract: Ceramide-1-phosphate (C1P) is a potential signaling molecule that modulates various cellular functions in animals. It has been known that C1P with different N-acyl lengths induce biological responses differently. However, molecular species profiles of the C1P in animal tissues have not been extensively examined yet. Here, we developed a method for determination of the molecular species of a C1P using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry with Phos-tag, a phosphate capture molecule. The amounts of total C1P in skin, brain, liver, kidney and small intestine of mice were determined to be 344, 151, 198, 96 and 90 pmol/g wet weight, respectively. We found a C1P species having an α-hydroxypalmitoyl residue (h-C1P, 44 pmol/g wet weight) in mouse skin. The h-C1P was detected only in the skin, and not other tissues of mice. The same analysis was applied to sphingomyelin after conversion of sphingomyelin to C1P by Streptomyces chromofuscus phospholipase D. We found that molecular species profiles of sphingomyelin in skin, kidney and small intestine of mice were similar to those of C1P in corresponding tissues. In contrast, molecular species profiles of sphingomyelin in liver and brain were quite different from those of C1P in these tissues, indicating selective synthesis or degradation of C1P in these tissues. The method described here will be useful for detection of changes in molecular species profiles of C1P and sphingomyelin.
authors.affiliation: Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, 770-8505, Japan.
authors.forename: Ryouhei
authors.initials: R
authors.lastname: Yamashita
authors.affiliation: Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, 770-8505, Japan.
authors.forename: Yumika
authors.initials: Y
authors.lastname: Tabata
authors.affiliation: Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, 770-8505, Japan.
authors.forename: Erina
authors.initials: E
authors.lastname: Iga
authors.affiliation: Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, 770-8505, Japan.
authors.forename: Michiyasu
authors.initials: M
authors.lastname: Nakao
authors.affiliation: Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, 770-8505, Japan.
authors.forename: Shigeki
authors.initials: S
authors.lastname: Sano
authors.affiliation: Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, 770-8505, Japan.
authors.forename: Kentaro
authors.initials: K
authors.lastname: Kogure
authors.affiliation: Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, 770-8505, Japan.|Department of Pharmaceutical Life Sciences, Faculty of Pharmacy, Yasuda Women's University, Hiroshima, 731-0351, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Tokumura
authors.affiliation: Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, 770-8505, Japan. tanaka.tamotsu@tokushima-u.ac.jp.
authors.forename: Tamotsu
authors.initials: T
authors.lastname: Tanaka
mesh_terms: D000818:Animals; D001923:Brain Chemistry; D002518:Ceramides / Q000737:chemistry* / Q000145:classification; D007421:Intestine, Small / Q000737:chemistry; D007668:Kidney / Q000737:chemistry; D008099:Liver / Q000737:chemistry / Q000378:metabolism; D051379:Mice; D012867:Skin / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013109:Sphingomyelins / Q000737:chemistry* / Q000145:classification; D014018:Tissue Distribution
chemical_list: D002518:Ceramides; D013109:Sphingomyelins; C065576:ceramide 1-phosphate
keywords: Ceramide-1-phosphate; Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry; Molecular species; Sphingomyelin