title: Evidence for Mitochondrial UPR Gene Activation in Familial and Sporadic Alzheimer's Disease.
abstract: Mitochondrial perturbations such as oxidative stress, increased fission/fusion dysfunction, and mitophagy are consistent features of Alzheimer's disease (AD), yet the mechanisms that initiate these perturbations are unclear. One potential source for mitochondrial defects could be an imbalance in mitochondrial proteostasis. In this regard, studies indicate that a specialized mitochondrial unfolded protein response (mtUPR) is activated upon the aberrant accumulation of damaged or unfolded proteins in the mitochondrial matrix, resulting in the up-regulation of key genes involved in mitochondrial stabilization. To test whether mtUPR activation occurs in AD, we performed real-time quantitative PCR on postmortem frontal cortex samples from subjects classified as sporadic AD, familial AD linked to presenilin-1 mutations, or cognitively intact controls. Compared to controls, sporadic AD subjects exhibited a significant ~40-60% increase in expression levels of select genes activated by the mtUPR, including mitochondrial chaperones dnaja3, hspd1, and hspe1, mitochondrial proteases clpp and yme1l1, and txn2, a mitochondrial-specific oxidoreductase. Furthermore, levels of all six mtUPR genes were significantly up-regulated by ~70-90% in familial AD compared to controls, and these expression levels were significantly higher compared to sporadic AD. The increase in hspd1 (Hsp60) was validated by western blotting. These data support the concept that both sporadic and familial AD are characterized by mtUPR gene activation. Understanding the physiological consequences of this response may provide subcellular mechanistic clues to selective neuronal vulnerability or endogenous compensatory mechanisms during the progression of AD.
authors.forename: John S
authors.initials: JS
authors.lastname: Beck
authors.forename: Elliott J
authors.initials: EJ
authors.lastname: Mufson
authors.affiliation: Michigan State University, 333 Bostwick Ave NE, Grand Rapids, MI 49503, USA. scott.counts@hc.msu.edu.
authors.forename: Scott E
authors.initials: SE
authors.lastname: Counts
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000235:genetics* / Q000378:metabolism*; D015153:Blotting, Western; D005190:Family; D005625:Frontal Lobe / Q000378:metabolism*; D050259:Genes, Mitochondrial / Q000502:physiology*; D006801:Humans; D008609:Mental Status Schedule; D008875:Middle Aged; D008928:Mitochondria / Q000235:genetics / Q000378:metabolism; D016133:Polymerase Chain Reaction; D015533:Transcriptional Activation; D056811:Unfolded Protein Response / Q000235:genetics* / Q000502:physiology*