title: Infection studies of nontarget mammalian cell lines with Bombyx mori macula-like virus.
abstract: Bombyx mori-derived cell lines are generally used for Bombyx mori nucleopolyhedrovirus (BmNPV)-based baculovirus expression vector system (BEVS). However, almost all of the B. mori-derived cell lines are persistently infected with Bombyx mori macula-like virus (BmMLV). In this study, nontarget mammalian cell lines were exposed to BmMLV, and their susceptibility was investigated. Real-time PCR showed that viral RNA in virus-inoculated nine mammalian cell lines decreased sharply at 7 days postinfection. Also, there was no significant effect on cell viability of mammalian cells after inoculation with BmMLV. These findings indicate that mammalian cell lines used in this study are not permissive to BmMLV, and BmMLV contamination might not affect the safety aspect of BmNPV-based BEVS.
authors.affiliation: Department of Agrobiology and Bioresources, Faculty of Agriculture, Utsunomiya University, Mine-machi 350, Utsunomiya-shi, Tochigi 321-8505, Japan.
authors.forename: Katsuhisa
authors.initials: K
authors.lastname: Innami
authors.affiliation: Department of Agrobiology and Bioresources, Faculty of Agriculture, Utsunomiya University, Mine-machi 350, Utsunomiya-shi, Tochigi 321-8505, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Aizawa
authors.affiliation: Animal Life Science Laboratory, Nippon Zenyaku Kogyo Co., Ltd., 1-1 Tairanoue Sasagawa, Asaka, Koriyama, Fukushima 963-0196, Japan.
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Tsukui
authors.affiliation: Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-8657, Japan.
authors.forename: Susumu
authors.initials: S
authors.lastname: Katsuma
authors.affiliation: Genebank, National Institute of Agrobiological Science, Kannondai 2-1-2, Tsukuba-shi, Ibaraki 305-8602, Japan.
authors.forename: Shigeo
authors.initials: S
authors.lastname: Imanishi
authors.affiliation: Department of Agrobiology and Bioresources, Faculty of Agriculture, Utsunomiya University, Mine-machi 350, Utsunomiya-shi, Tochigi 321-8505, Japan.
authors.forename: Hideki
authors.initials: H
authors.lastname: Kawasaki
authors.affiliation: Department of Agrobiology and Bioresources, Faculty of Agriculture, Utsunomiya University, Mine-machi 350, Utsunomiya-shi, Tochigi 321-8505, Japan. Electronic address: iwanaga@cc.utsunomiya-u.ac.jp.
authors.forename: Masashi
authors.initials: M
authors.lastname: Iwanaga
mesh_terms: D000818:Animals; D012831:Bombyx / Q000821:virology*; D002460:Cell Line; D002470:Cell Survival; D058507:Host Specificity; D008322:Mammals; D012367:RNA, Viral / Q000032:analysis; D060888:Real-Time Polymerase Chain Reaction; D046830:Tymoviridae / Q000254:growth & development*; D014776:Virus Cultivation
chemical_list: D012367:RNA, Viral
keywords: BEVS; BmMLV; BmNPV; Maculavirus; Mammalian cell line