title: Improving 3'-Hydroxygenistein Production in Recombinant Pichia pastoris Using Periodic Hydrogen Peroxide-Shocking Strategy.
abstract: 3'-Hydroxygenistein can be obtained from the biotransformation of genistein by the engineered Pichia pastoris X-33 strain, which harbors a fusion gene composed of CYP57B3 from Aspergillus oryzae and a cytochrome P450 oxidoreductase gene (sCPR) from Saccharomyces cerevisiae. P. pastoris X-33 mutants with higher 3'-hydroxygenistein production were selected using a periodic hydrogen peroxide-shocking strategy. One mutant (P2-D14-5) produced 23.0 mg/l of 3'-hydroxygenistein, representing 1.87-fold more than that produced by the recombinant X-33. When using a 5 L fermenter, the P2-D14-5 mutant produced 20.3 mg/l of 3'- hydroxygenistein, indicating a high potential for industrial-scale 3'-hydroxygenistein production.
authors.affiliation: Biodiversity Research Center, Academia Sinica, Taipei 115, Taiwan, Republic of China.
authors.forename: Tzi-Yuan
authors.initials: TY
authors.lastname: Wang
authors.affiliation: Department of Biological Sciences and Technology, National University of Tainan, Tainan, 702, Taiwan, Republic of China.
authors.forename: Yi-Hsuan
authors.initials: YH
authors.lastname: Tsai
authors.affiliation: Department of Biological Sciences and Technology, National University of Tainan, Tainan, 702, Taiwan, Republic of China.
authors.forename: I-Zen
authors.initials: IZ
authors.lastname: Yu
authors.affiliation: Department of Biological Sciences and Technology, National University of Tainan, Tainan, 702, Taiwan, Republic of China.
authors.forename: Te-Sheng
authors.initials: TS
authors.lastname: Chang
mesh_terms: D003470:Culture Media / Q000378:metabolism; D005285:Fermentation; D006861:Hydrogen Peroxide / Q000378:metabolism*; D060847:Metabolic Engineering; D010843:Pichia / Q000235:genetics / Q000378:metabolism*; D010881:Piperidones; D011743:Pyrimidines / Q000096:biosynthesis*
chemical_list: C478008:3'-hydroxygepirone; D003470:Culture Media; D010881:Piperidones; D011743:Pyrimidines; D006861:Hydrogen Peroxide
keywords: 3'-Hydroxygenistein; CYP57B3; Pichia pastoris; orobol