title: iTRAQ-based quantitative proteomic analysis gives insight into sexually different metabolic processes of poplars under nitrogen and phosphorus deficiencies.
abstract: Male and female poplars (Populus cathayana Rehd.) respond differently to nitrogen (N) and phosphorus (P) deficiencies. In this study, an iTRAQ-based quantitative proteomic analysis was performed. N and P deficiencies caused 189 and 144 proteins to change in abundance in males and 244 and 464 in females, respectively. Compared to N- and P-deficient males, both N- and P-deficient females showed a wider range of changes in proteins that are involved in amino acid, carbohydrate and protein metabolism, and the sexual differences were significant. When comparing the effects of N- and P-deficiencies, N-deficient females expressed more changes in proteins that are involved in stress responses and gene expression regulation, while P-deficient females showed more changes in proteins that are involved in energy and lipid metabolism, stress responses and gene expression regulation. The quantitative RT-PCR analysis of stress-related proteins showed that males have a better expression correlation between mRNA and protein levels than do females. This study shows that P. cathayana females are more sensitive and have more rapid metabolic mechanisms when responding to N and P deficiencies than do males, and P deficiency has a wider range of effects on females than does N deficiency.
authors.affiliation: Key Laboratory of Mountain Surface Processes and Ecological Regulation, Institute of Mountain Hazards and Environment, Chinese Academy of Sciences, Chengdu, P. R. China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Mountain Surface Processes and Ecological Regulation, Institute of Mountain Hazards and Environment, Chinese Academy of Sciences, Chengdu, P. R. China.|University of Chinese Academy of Sciences, Beijing, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Mountain Surface Processes and Ecological Regulation, Institute of Mountain Hazards and Environment, Chinese Academy of Sciences, Chengdu, P. R. China.
authors.forename: Hongxia
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Department of Agricultural Sciences, Viikki Plant Science Centre, University of Helsinki, Helsinki, Finland.
authors.forename: Helena
authors.initials: H
authors.lastname: Korpelainen
authors.affiliation: Key Laboratory of Mountain Surface Processes and Ecological Regulation, Institute of Mountain Hazards and Environment, Chinese Academy of Sciences, Chengdu, P. R. China.|The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, Lin'an, Zhejiang, P. R. China.
authors.forename: Chunyang
authors.initials: C
authors.lastname: Li
mesh_terms: D002245:Carbon Dioxide / Q000378:metabolism; D002852:Chromatography, Ion Exchange; D018506:Gene Expression Regulation, Plant; D009584:Nitrogen / Q000378:metabolism*; D010758:Phosphorus / Q000378:metabolism*; D010788:Photosynthesis; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D032107:Populus / Q000235:genetics / Q000502:physiology*; D040901:Proteomics; D021241:Spectrometry, Mass, Electrospray Ionization; D013312:Stress, Physiological
chemical_list: D010940:Plant Proteins; D002245:Carbon Dioxide; D010758:Phosphorus; D009584:Nitrogen
keywords: Nitrogen deficiency; Phosphorus deficiency; Plant proteomics; Poplar; Sexual differences; iTRAQ