title: Caryophyllene sesquiterpenoids from the endophytic fungus, Pestalotiopsis sp.
abstract: Eight new caryophyllene sesquiterpenoids named pestaloporinates A-G (1-7) and 14-acetylhumulane (8) have been isolated from the solid cultures of an endophytic fungus Pestalotiopsis sp., which was obtained from the fresh stem bark of Melia azedarach Linn. Their structures as well as absolute configurations were determined by spectroscopic data, ECD experimentation, and single-crystal X-ray diffraction. Among all the isolates, compound 2 displayed potent inhibitory activity with IC50 value of 19.0 μM during the evaluation of nitric oxide (NO) inhibition in lipopolysaccharide (LPS)-induced RAW 264.7 macrophage cells.
authors.affiliation: State Key Laboratory of Natural Medicines, Department of Natural Medicinal Chemistry, China Pharmaceutical University, Nanjing 210009, People's Republic of China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Natural Medicines, Department of Natural Medicinal Chemistry, China Pharmaceutical University, Nanjing 210009, People's Republic of China. Electronic address: skeepjack@163.com.
authors.forename: Ming-Hua
authors.initials: MH
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Natural Medicines, Department of Natural Medicinal Chemistry, China Pharmaceutical University, Nanjing 210009, People's Republic of China.
authors.forename: Xiao-Bing
authors.initials: XB
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Natural Medicines, Department of Natural Medicinal Chemistry, China Pharmaceutical University, Nanjing 210009, People's Republic of China.
authors.forename: Tian-Xiao
authors.initials: TX
authors.lastname: Li
authors.affiliation: State Key Laboratory of Natural Medicines, Department of Natural Medicinal Chemistry, China Pharmaceutical University, Nanjing 210009, People's Republic of China. Electronic address: cpu_lykong@126.com.
authors.forename: Ling-Yi
authors.initials: LY
authors.lastname: Kong
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification; D008264:Macrophages / Q000187:drug effects; D031586:Melia azedarach / Q000382:microbiology; D051379:Mice; D015394:Molecular Structure; D009569:Nitric Oxide / Q000378:metabolism; D024301:Plant Bark / Q000382:microbiology; D000081223:Polycyclic Sesquiterpenes; D000067996:RAW 264.7 Cells; D012717:Sesquiterpenes / Q000737:chemistry* / Q000302:isolation & purification; D013098:Xylariales / Q000737:chemistry*
chemical_list: D000893:Anti-Inflammatory Agents; D000081223:Polycyclic Sesquiterpenes; D012717:Sesquiterpenes; C000608983:pestaloporinate B; D009569:Nitric Oxide; C024714:caryophyllene
keywords: Caryophyllene sesquiterpenoids; Ethyl ethanoate (PubChem CID: 8857); Methanol (PubChem CID: 887); Methyl cyanide (PubChem CID: 6342); Methylene chloride (PubChem CID: 6344); NO inhibition; Pestalotiopsis sp.; Pyridine (PubChem CID: 1049)