title: Saccharothrix stipaesp. nov., an actinomycete isolated from the rhizosphere ofStipa grandis.
abstract: An actinomycete, strain D34T, was isolated from a soil sample collected from the rhizosphere ofStipa grandisat Yunwu Mountain in Ningxia, north-west China. Strain D34Tshowed highest 16S rRNA gene sequence similarity toSaccharothrix espanaensisDSM 44229T(99.0 %),Saccharothrix texasensisNRRL B-16107T(98.7 %) andSaccharothrix variisporeaNRRL B-16296T(98.6 %). The strain containedmeso-diaminopimelic acid, alanine, glycine and glutamic acid as major cell-wall amino acids. Mannose, rhamnose and galactose were the characteristic whole-cell sugars. The fatty acid profile consisted predominantly of iso-C15 : 0, iso-C16 : 0, iso-C16 : 1, C17 : 1ω6c, anteiso-C17 : 0and anteiso-C15 : 0. The phospholipid profile included phosphatidylethanolamine (typical of phospholipid pattern type II). Furthermore, a combination of some physiological and biochemical properties and low DNA-DNA relatedness values indicated that strain D34Twas differentiated from members of closely related species. On the basis of these phenotypic, genotypic and chemotaxonomic data, strain D34Trepresents a novel species of the genusSaccharothrix, for which the nameSaccharothrix stipaesp. nov. is proposed. The type strain is D34T( = JCM 30560T= ACCC19714T).
authors.affiliation: Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, Northwest A&F University, Yangling 712100, PR China.|College of Life Sciences, State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A&F University, Yangling Shaanxi 712100, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Bing Lin
authors.affiliation: College of Life Sciences, State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A&F University, Yangling Shaanxi 712100, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Qing Guo
authors.affiliation: College of Life Sciences, State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A&F University, Yangling Shaanxi 712100, PR China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Hui Di
authors.affiliation: College of Life Sciences, State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A&F University, Yangling Shaanxi 712100, PR China.
authors.forename: Miao
authors.initials: M
authors.lastname: Chun Fan
authors.affiliation: College of Life Sciences, State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A&F University, Yangling Shaanxi 712100, PR China.
authors.forename: Dan
authors.initials: D
authors.lastname: Hong Dong
authors.affiliation: Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, Northwest A&F University, Yangling 712100, PR China.|College of Life Sciences, State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A&F University, Yangling Shaanxi 712100, PR China.
authors.forename: Ge
authors.initials: G
authors.lastname: Hong Wei