title: ATP1B3 Protein Modulates the Restriction of HIV-1 Production and Nuclear Factor κ Light Chain Enhancer of Activated B Cells (NF-κB) Activation by BST-2.
abstract: Here, we identify ATP1B3 and fibrillin-1 as novel BST-2-binding proteins. ATP1B3 depletion in HeLa cells (BST-2-positive cells), but not 293T cells (BST-2-negative cells), induced the restriction of HIV-1 production in a BST-2-dependent manner. In contrast, fibrillin-1 knockdown reduced HIV-1 production in 293T and HeLa cells in a BST-2-independent manner. Moreover, NF-κB activation was enhanced by siATP1B3 treatment in HIV-1- and HIV-1ΔVpu-infected HeLa cells. In addition, ATP1B3 silencing induced high level BST-2 expression on the surface of HeLa cells. These results indicate that ATP1B3 is a co-factor that accelerates BST-2 degradation and reduces BST-2-mediated restriction of HIV-1 production and NF-κB activation.
authors.affiliation: From the Research Center for Hepatitis and Immunology, National Center for Global Health and Medicine, Ichikawa, Chiba 272-8516.
authors.forename: Hironori
authors.initials: H
authors.lastname: Nishitsuji
authors.affiliation: the Department of Life and Environmental Sciences and the Department of Microbiology, Yokohama City University School of Medicine, Kanagawa 236-0004, Japan.
authors.forename: Ryuichi
authors.initials: R
authors.lastname: Sugiyama
authors.affiliation: the Department of Life and Environmental Sciences and.
authors.forename: Makoto
authors.initials: M
authors.lastname: Abe
authors.affiliation: the Department of Life and Environmental Sciences and Research Institute, Chiba Institute of Technology, 2-17-1 Tsudanuma, Narashino-shi, Chiba 275-0016, Japan, and hiroshi.takaku@it-chiba.ac.jp hiroshi.takaku@p.chibakoudai.jp.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Takaku
mesh_terms: D015703:Antigens, CD / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D000071838:Fibrillin-1; D000071837:Fibrillins; D058851:GPI-Linked Proteins / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017353:Gene Deletion; D005786:Gene Expression Regulation; D057809:HEK293 Cells; D015497:HIV-1 / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D008840:Microfilament Proteins / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D016328:NF-kappa B / Q000819:agonists / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D054730:Protein Interaction Domains and Motifs; D055550:Protein Stability; D059748:Proteolysis; D034622:RNA Interference; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020798:Two-Hybrid System Techniques; D056189:Viral Tropism; D014779:Virus Replication; D000074010:Bone Marrow Stromal Antigen 2; D054392:Adipokines
chemical_list: D015703:Antigens, CD; D000071838:Fibrillin-1; D000071837:Fibrillins; D058851:GPI-Linked Proteins; D008840:Microfilament Proteins; D016328:NF-kappa B; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D000254:Sodium-Potassium-Exchanging ATPase; C519304:ATP1B3 protein, human; C094572:BST2 protein, human; D000074010:Bone Marrow Stromal Antigen 2; C000605722:FBN1 protein, human; D054392:Adipokines
keywords: ATP1B3; BST-2; NF-kappa B (NF-KB); Na+/K+-ATPase; bone marrow; cell biology; cell surface protein; human immunodeficiency virus (HIV); membrane trafficking; retrovirus