title: Evaluating the Use of Commercial West Nile Virus Antigens as Positive Controls in the Rapid Analyte Measurement Platform West Nile Virus Assay.
abstract: We evaluated the utility of 2 types of commercially available antigens as positive controls in the Rapid Analyte Measurement Platform (RAMP®) West Nile virus (WNV) assay. Purified recombinant WNV envelope antigens and whole killed virus antigens produced positive RAMP results and either type would be useful as a positive control. Killed virus antigens provide operational and economic advantages and we recommend their use over purified recombinant antigens. We also offer practical applications for RAMP positive controls and recommendations for preparing them.
authors.affiliation: Division of Vector-Borne Diseases, Centers for Disease Control and Prevention, 3156 Rampart Road, Fort Collins, CO 80521.
authors.forename: Kristen L
authors.initials: KL
authors.lastname: Burkhalter
authors.affiliation: Division of Vector-Borne Diseases, Centers for Disease Control and Prevention, 3156 Rampart Road, Fort Collins, CO 80521.
authors.forename: Harry M
authors.initials: HM
authors.lastname: Savage
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000276:immunology*; D009033:Culicidae / Q000821:virology*; D007118:Immunoassay / Q000379:methods* / Q000592:standards; D007303:Insect Vectors / Q000821:virology*; D016133:Polymerase Chain Reaction; D015203:Reproducibility of Results; D014901:West Nile Fever / Q000517:prevention & control; D014902:West Nile virus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*
chemical_list: D000956:Antigens, Viral
keywords: Rapid Analyte Measurement Platform® test; West Nile virus; commercial antigen; positive control