title: Reciprocal regulation of Hsa-miR-1 and long noncoding RNA MALAT1 promotes triple-negative breast cancer development.
abstract: Recent studies demonstrated that long noncoding RNAs (lncRNAs) have a critical role in the regulation of cancer progression and metastasis. However, little is known about the mechanism through which metastasis-associated lung adencarcinoma transcript 1 (MALAT1) exerts its oncogenic activity, and the interaction between MALAT1 and microRNA remains largely unknown. In the present study, we reported that MALAT1 was upregulated in triple-negative breast cancer (TNBC) tissues. Knockdown of MALAT1 inhibited proliferation, motility, and increased apoptosis in vitro. In vivo study indicated that knockdown of MALAT1 inhibited tumor growth and metastasis. Patients with high MALAT1 expression had poorer overall survival time than those with low MALAT1 expression. In addition, our findings demonstrate a reciprocal negative control relationship between MALAT1 and miR-1: downregulation of MALAT1 increased expression of microRNA-1 (miR-1), while overexpression of miR-1 decreased MALAT1 expression. Slug was identified as a direct target of miR-1. We proposed that MALAT1 exerted its function through the miR-1/slug axis. In summary, we proposed that MALAT1 may be a target for TNBC therapy.
authors.affiliation: The Affiliated Cancer Hospital of Guangzhou Medical University, Guangzhou, China.
authors.forename: Chuan
authors.initials: C
authors.lastname: Jin
authors.affiliation: The Affiliated Cancer Hospital of Guangzhou Medical University, Guangzhou, China.
authors.forename: Bingchuan
authors.initials: B
authors.lastname: Yan
authors.affiliation: The Affiliated Cancer Hospital of Guangzhou Medical University, Guangzhou, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Lu
authors.affiliation: Oncology Center, The Affiliated Hospital of Guangdong Medical College, Zhanjiang, China.
authors.forename: Yanmin
authors.initials: Y
authors.lastname: Lin
authors.affiliation: The Affiliated Cancer Hospital of Guangzhou Medical University, Guangzhou, China. leima_01@yeah.net.
authors.forename: Lei
authors.initials: L
authors.lastname: Ma
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D000818:Animals; D045744:Cell Line, Tumor; D004274:DNA, Recombinant / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053208:Kaplan-Meier Estimate; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics*; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D012313:RNA / Q000235:genetics; D034622:RNA Interference; D062085:RNA, Long Noncoding / Q000096:biosynthesis / Q000235:genetics*; D012334:RNA, Neoplasm / Q000096:biosynthesis / Q000235:genetics*; D034741:RNA, Small Interfering / Q000235:genetics; D000071250:Snail Family Transcription Factors / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D013047:Specific Pathogen-Free Organisms; D064726:Triple Negative Breast Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000401:mortality / Q000473:pathology
chemical_list: D020413:3' Untranslated Regions; D004274:DNA, Recombinant; C557041:MALAT1 long non-coding RNA, human; C544547:MIRN1 microRNA, human; D035683:MicroRNAs; D009363:Neoplasm Proteins; D062085:RNA, Long Noncoding; D012334:RNA, Neoplasm; D034741:RNA, Small Interfering; C039919:RNA, recombinant; C000604407:SNAI1 protein, human; D000071250:Snail Family Transcription Factors; D012313:RNA
keywords: MALAT1; Slug; Triple-negative breast cancer; miR-1