title: An Assessment of Cell Culture Plate Surface Chemistry for in Vitro Studies of Tissue Engineering Scaffolds.
abstract: The use of biopolymers as a three dimensional (3D) support structure for cell growth is a leading tissue engineering approach in regenerative medicine. Achieving consistent cell seeding and uniform cell distribution throughout 3D scaffold culture in vitro is an ongoing challenge. Traditionally, 3D scaffolds are cultured within tissue culture plates to enable reproducible cell seeding and ease of culture media change. In this study, we compared two different well-plates with different surface properties to assess whether seeding efficiencies and cell growth on 3D scaffolds were affected. Cell attachment and growth of murine calvarial osteoblast (MC3T3-E1) cells within a melt-electrospun poly-ε-caprolactone scaffold were assessed when cultured in either "low-adhesive" non-treated or corona discharged-treated well-plates. Increased cell adhesion was observed on the scaffold placed in the surface treated culture plates compared to the scaffold in the non-treated plates 24 h after seeding, although it was not significant. However, higher cell metabolic activity was observed on the bases of all well-plates than on the scaffold, except for day 21, well metabolic activity was higher in the scaffold contained in non-treated plate than the base. These results indicate that there is no advantage in using non-treated plates to improve initial cell seeding in 3D polymeric tissue engineering scaffolds, however non-treated plates may provide an improved metabolic environment for long-term studies.
authors.affiliation: Institute of Health & Biomedical Innovation, Queensland University of Technology (QUT), Brisbane, Queensland, 4059, Australia. alexroeder89@gmail.com.
authors.forename: Alexander
authors.initials: A
authors.lastname: Röder
authors.affiliation: Institute of Health & Biomedical Innovation, Queensland University of Technology (QUT), Brisbane, Queensland, 4059, Australia. GarciaE@raft.ac.uk.|RAFT Institute of Plastic Surgery, Mount Vernon Hospital, Northwood HA6 2RN, UK. GarciaE@raft.ac.uk.
authors.forename: Elena
authors.initials: E
authors.lastname: García-Gareta
authors.affiliation: Institute of Health & Biomedical Innovation, Queensland University of Technology (QUT), Brisbane, Queensland, 4059, Australia. c.theodoropoulos@qut.edu.au.
authors.forename: Christina
authors.initials: C
authors.lastname: Theodoropoulos
authors.affiliation: Institute of Health & Biomedical Innovation, Queensland University of Technology (QUT), Brisbane, Queensland, 4059, Australia. nikola.ristovski@hdr.qut.edu.au.
authors.forename: Nikola
authors.initials: N
authors.lastname: Ristovski
authors.affiliation: Institute of Health & Biomedical Innovation, Queensland University of Technology (QUT), Brisbane, Queensland, 4059, Australia. Raxxman@gmail.com.
authors.forename: Keith A
authors.initials: KA
authors.lastname: Blackwood
authors.affiliation: Institute of Health & Biomedical Innovation, Queensland University of Technology (QUT), Brisbane, Queensland, 4059, Australia. mia.woodruff@qut.edu.au.
authors.forename: Maria A
authors.initials: MA
authors.lastname: Woodruff
keywords: 3D scaffolds; cell attachment; poly-ε-caprolactone; tissue engineering