title: Identification, Purification, and Characterization of Staphylococcal Superantigens.
abstract: Purifying natively produced staphylococcal superantigens is an important process in the study of these proteins, as many common methods of protein purification are affected by staphylococcal protein A contamination. Here, we describe a proven approach for identifying superantigens in vitro as well as for purifying novel superantigens both in His-tagged and native forms using modern genetic tools coupled with thin-layer isoelectric focusing.
authors.affiliation: Department of Microbiology, University of Iowa, 51 Newton Road, Bowen Science Building 3-403, Iowa City, IA, 52242, USA.
authors.forename: Joseph A
authors.initials: JA
authors.lastname: Merriman
authors.affiliation: Department of Microbiology, University of Iowa, 51 Newton Road, Bowen Science Building 3-403, Iowa City, IA, 52242, USA. patrick-schlievert@uiowa.edu.
authors.forename: Patrick M
authors.initials: PM
authors.lastname: Schlievert
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology; D011817:Rabbits; D013210:Staphylococcus / Q000276:immunology*; D018089:Superantigens / Q000276:immunology* / Q000302:isolation & purification*
chemical_list: D000907:Antibodies, Bacterial; D018089:Superantigens
keywords: Characterization; Identification; Polyclonal antibody production; Purification; Staphylococcal superantigen