title: Strain-level diversity of secondary metabolism in the biocontrol species Aneurinibacillus migulanus.
abstract: Aneurinibacillus migulanus strains Nagano and NCTC 7096 show potential in biocontrol against fungal and fungus-like plant pathogens, including a wide range of Ascomycota, Basidiomycota, and Oomycetes. Differences in terms of the range of pathogens that each strain inhibits, however, suggested that production of a single antibiotic cyclic peptide, gramicidin S (GS), by the two strains, is not the sole mechanism of inhibition. The availability of four sequenced genomes of Aneurinibacillus prompted us to apply genome mining techniques to identify the bioactive potential of A. migulanus and to provide insights into the secondary metabolite arsenal of the genus Aneurinibacillus. Up to eleven secondary metabolite biosynthetic gene clusters were present in the three Aneurinibacillus species. Biosynthetic gene clusters specifying bacteriocins, microcins, non-ribosomal peptides, polyketides, terpenes, phosphonates, lasso peptides and linaridins were identified. Chitinolytic potential and iron metabolism regulation were also investigated. With increasing numbers of biocontrol bacterial genomes being sequenced and mined, the use of approaches similar to those described in this paper will lead to an increase in the numbers of environmentally friendly natural products available to use against plant diseases.
authors.affiliation: University of Aberdeen, Institute of Biological and Environment Science, Cruickshank Building, St. Machar Drive, Aberdeen AB24 3UU Scotland, UK; NextBiotech, 98 Rue Ali Belhouane, 3030 Agareb, Tunisia.
authors.forename: Faizah N
authors.initials: FN
authors.lastname: Alenezi
authors.affiliation: NextBiotech, 98 Rue Ali Belhouane, 3030 Agareb, Tunisia.
authors.forename: Imen
authors.initials: I
authors.lastname: Rekik
authors.affiliation: University of Aberdeen, Institute of Biological and Environment Science, Cruickshank Building, St. Machar Drive, Aberdeen AB24 3UU Scotland, UK; Poznań University of Life Sciences, Wojska Polskiego 71C, 60-625 Poznań, Poland.
authors.forename: Marta
authors.initials: M
authors.lastname: Bełka
authors.affiliation: University of Aberdeen, Institute of Biological and Environment Science, Cruickshank Building, St. Machar Drive, Aberdeen AB24 3UU Scotland, UK.
authors.forename: Abrar F
authors.initials: AF
authors.lastname: Ibrahim
authors.affiliation: NextBiotech, 98 Rue Ali Belhouane, 3030 Agareb, Tunisia; University of Veterinary Med. & Pharmacy, Institute of Biology, Zoology & Radiobiology, Department of Biology and Genetics, Komenského 73, 04181 Kosice, Slovakia.
authors.forename: Lenka
authors.initials: L
authors.lastname: Luptakova
authors.affiliation: University of Aberdeen, Marine Biodiscovery Centre, Department of Chemistry, Aberdeen AB24 3UE, UK.
authors.forename: Marcel
authors.initials: M
authors.lastname: Jaspars
authors.affiliation: University of Aberdeen, Institute of Biological and Environment Science, Cruickshank Building, St. Machar Drive, Aberdeen AB24 3UU Scotland, UK; NextBiotech, 98 Rue Ali Belhouane, 3030 Agareb, Tunisia.
authors.forename: Steve
authors.initials: S
authors.lastname: Woodward
authors.affiliation: NextBiotech, 98 Rue Ali Belhouane, 3030 Agareb, Tunisia; Laboratory of Soil Biology, University of Neuchatel, 11 Rue Emile Argand, CH-2000 Neuchatel, Switzerland. Electronic address: lassaad.belbahri@unine.ch.
authors.forename: Lassaad
authors.initials: L
authors.lastname: Belbahri
mesh_terms: D000595:Amino Acid Sequence; D000935:Antifungal Agents / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D056449:Bacillales / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D005658:Fungi / Q000187:drug effects; D008969:Molecular Sequence Data; D064210:Secondary Metabolism; D016415:Sequence Alignment; D013045:Species Specificity
chemical_list: D000935:Antifungal Agents
keywords: Aneurinibacillus migulanus; Biocontrol bacteria; Bioinformatics; Genome mining; Secondary metabolism