title: Usefulness of Dried Blood Spots (DBS) to perform hepatitis C virus genotyping in drug users in Senegal.
abstract: The aim of this pilot study was to analyze the Hepatitis C Virus (HCV) genotypes circulating in Senegal among Drug User (DUs), using Dried Blood Spots (DBS) as RNA source for molecular assays. Heroin and/or cocaine users (n = 506) were recruited in Dakar from April to July 2011, using a Respondent Driven Sampling (RDS) method. DBS preparation consisted of five drops of whole blood from finger applied to a Whatman paper card. HCV infection was screened by the detection of anti-HCV antibodies, using a rapid immune-chromatographic test. HCV RNA was quantified on anti-HCV positive DBS, using the Abbott RealTime HCV® Genotyping was performed on DBS with detectable viral load with Versant® HCV Genotype 2.0 Assay (LiPA) and Abbott RealTime HCV Genotype II assay®. Among the 506 participants, 120 were tested as positive for anti-HCV antibodies and their samples were analyzed for HCV RNA viral load and genotype. Out of the 120 DBS tested, HCV RNA was detected on 25 (20.8%). The median viral load was 15,058 IU/ml (ranging from 710 to 766,740 IU/ml). All positive DBS were suitable for the genotyping assay, that showed a predominance of genotype 1 (21/25) including 16 genotypes 1a and 5 genotypes 1b. HCV genotype 1 prevails in a DU population in Dakar. DBS could be useful for HCV RNA genotyping, but optimal storage conditions should required avoiding RNA impairment. Acknowledging this limitation, DBS could be a great interest for detecting and genotyping HCV viremic patients. J. Med. Virol. 89:484-488, 2017. © 2015 Wiley Periodicals, Inc.
authors.affiliation: Bacteriology-Virology laboratory, CHU A. le Dantec, Cheikh Anta Diop University of Dakar, Dakar, Senegal.|Regional center for research and training on HIV/AIDS CHU Fann, Dakar, Senegal.
authors.forename: O
authors.initials: O
authors.lastname: Ndiaye
authors.affiliation: Saint Antoine Hospital, Paris, France.
authors.forename: J
authors.initials: J
authors.lastname: Gozlan
authors.affiliation: Bacteriology-Virology laboratory, CHU A. le Dantec, Cheikh Anta Diop University of Dakar, Dakar, Senegal.
authors.forename: H
authors.initials: H
authors.lastname: Diop-Ndiaye
authors.affiliation: Bacteriology-Virology laboratory, CHU A. le Dantec, Cheikh Anta Diop University of Dakar, Dakar, Senegal.
authors.forename: A S
authors.initials: AS
authors.lastname: Sall
authors.affiliation: Saint Antoine Hospital, Paris, France.
authors.forename: S
authors.initials: S
authors.lastname: Chapelain
authors.affiliation: Institute of Medecine and Applied Epidemiology-IMEA, Paris, France.
authors.forename: A
authors.initials: A
authors.lastname: Leprêtre
authors.affiliation: Regional center for research and training on HIV/AIDS CHU Fann, Dakar, Senegal.
authors.forename: M
authors.initials: M
authors.lastname: Maynart
authors.affiliation: Bacteriology-Virology laboratory, CHU A. le Dantec, Cheikh Anta Diop University of Dakar, Dakar, Senegal.
authors.forename: M
authors.initials: M
authors.lastname: Gueye
authors.affiliation: Bacteriology-Virology laboratory, CHU A. le Dantec, Cheikh Anta Diop University of Dakar, Dakar, Senegal.
authors.forename: G
authors.initials: G
authors.lastname: Lo
authors.affiliation: Bacteriology-Virology laboratory, CHU A. le Dantec, Cheikh Anta Diop University of Dakar, Dakar, Senegal.
authors.forename: M
authors.initials: M
authors.lastname: Thiam
authors.affiliation: Psychiatry Service, CHU Fann, Dakar, Senegal.
authors.forename: I
authors.initials: I
authors.lastname: Ba
authors.affiliation: Saint Antoine Hospital, Paris, France.
authors.forename: K
authors.initials: K
authors.lastname: Lacombe
authors.affiliation: Saint Antoine Hospital, Paris, France.
authors.forename: P M
authors.initials: PM
authors.lastname: Girard
authors.affiliation: Bacteriology-Virology laboratory, CHU A. le Dantec, Cheikh Anta Diop University of Dakar, Dakar, Senegal.
authors.forename: S
authors.initials: S
authors.lastname: Mboup
authors.affiliation: Bacteriology-Virology laboratory, CHU A. le Dantec, Cheikh Anta Diop University of Dakar, Dakar, Senegal.
authors.forename: C T
authors.initials: CT
authors.lastname: Kane
mesh_terms: D000293:Adolescent; D000328:Adult; D001769:Blood / Q000821:virology; D003890:Desiccation; D055030:Drug Users; D005260:Female; D060005:Genotyping Techniques / Q000379:methods*; D016174:Hepacivirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D006526:Hepatitis C / Q000175:diagnosis / Q000821:virology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010865:Pilot Projects; D012367:RNA, Viral / Q000097:blood*; D012675:Senegal; D013048:Specimen Handling / Q000379:methods*; D055815:Young Adult
chemical_list: D012367:RNA, Viral
keywords: DBS; HCV; Senegal; drug users