title: Evaluation of an Immunochromatographic Assay for Rapid Detection of Penicillin-Binding Protein 2a in Human and Animal Staphylococcus intermedius Group, Staphylococcus lugdunensis, and Staphylococcus schleiferi Clinical Isolates.
abstract: The performance of a rapid penicillin-binding protein 2a (PBP2a) detection assay, the Alere PBP2a culture colony test, was evaluated for identification of PBP2a-mediated beta-lactam resistance in human and animal clinical isolates of Staphylococcus intermedius group, Staphylococcus lugdunensis, and Staphylococcus schleiferi. The assay was sensitive and specific, with all PBP2a-negative and PBP2a-positive strains testing negative and positive, respectively.
authors.affiliation: Emory University School of Medicine, Atlanta, Georgia, USA.
authors.forename: A R
authors.initials: AR
authors.lastname: Arnold
authors.affiliation: Washington University in St. Louis School of Medicine, St. Louis, Missouri, USA.
authors.forename: C-A D
authors.identifier: http://orcid.org/0000-0002-1137-840X
authors.initials: CA
authors.lastname: Burnham
authors.affiliation: University of Iowa Hospitals and Clinics, Iowa City, Iowa, USA.
authors.forename: B A
authors.initials: BA
authors.lastname: Ford
authors.affiliation: College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, Texas, USA.
authors.forename: S D
authors.identifier: http://orcid.org/0000-0001-9154-8909
authors.initials: SD
authors.lastname: Lawhon
authors.affiliation: Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
authors.forename: S K
authors.initials: SK
authors.lastname: McAllister
authors.affiliation: Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
authors.forename: D
authors.initials: D
authors.lastname: Lonsway
authors.affiliation: Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
authors.forename: V
authors.initials: V
authors.lastname: Albrecht
authors.affiliation: Emory University School of Medicine, Atlanta, Georgia, USA Children's Healthcare of Atlanta, Atlanta, Georgia, USA.
authors.forename: R C
authors.initials: RC
authors.lastname: Jerris
authors.affiliation: Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
authors.forename: J K
authors.initials: JK
authors.lastname: Rasheed
authors.affiliation: Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
authors.forename: B
authors.initials: B
authors.lastname: Limbago
authors.affiliation: Emory University School of Medicine, Atlanta, Georgia, USA Emory Antibiotic Resistance Center, Atlanta, Georgia, USA eburd@emory.edu law9067@med.cornell.edu.
authors.forename: E M
authors.initials: EM
authors.lastname: Burd
authors.affiliation: Weill Cornell Medical College, New York, New York, USA eburd@emory.edu law9067@med.cornell.edu.
authors.forename: L F
authors.initials: LF
authors.lastname: Westblade
mesh_terms: D000818:Animals; D002846:Chromatography, Affinity / Q000379:methods / Q000592:standards; D006801:Humans; D046915:Penicillin-Binding Proteins / Q000378:metabolism*; D010453:Peptide Synthases / Q000378:metabolism*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013203:Staphylococcal Infections / Q000382:microbiology; D057789:Staphylococcus intermedius / Q000302:isolation & purification / Q000378:metabolism*; D057787:Staphylococcus lugdunensis / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D046915:Penicillin-Binding Proteins; C478699:penicillin-binding protein 2a, Streptococcus; D010453:Peptide Synthases