title: Ophthalmic indications of amniotic membrane transplantation in Mexico: an eight years Amniotic Membrane Bank experience.
abstract: Amniotic membrane, the inner layer of the placenta, has biological properties (e.g. promotes epithelization, reduces fibrosis, secretes antimicrobial products and inhibits immune responses) which make it a useful option for several ophthalmologic procedures, especially those involving the ocular surface. Its use in eye surgery has been reported by other authors. To our knowledge, there is a lack of descriptive studies on surgical indications using amniotic membrane in Mexican population. Here we describe the eight years Amniotic Membrane Bank experience in Mexico, including a detailed protocol of the donors selection, tissue harvesting, preparation, storage and distribution of amniotic membrane since its establishment in 2007. Moreover, we describe the Ophthalmological indications of amniotic membrane transplantation of the total of 1686 amniotic membranes fragments used during eight years. The five most common indications for amniotic membrane transplantation were pterygium (46 %), corneal ulcers (12.6 %), conjunctival surface repair (11.1 %), neoplasms (7.4 %), and persistent epithelial defects (7.3 %). In addition, we compared the indications of amniotic membrane use in two different types of Institutions: general hospitals and ophthalmologic reference hospitals. We found interesting differences between the indications and use rates between these institutions, although pterygium was the most frequent pathology that amniotic membrane fragments were used in both institutions, there was up to a five-fold increase in the use of amniotic membrane for correction of persistent epithelial defects in reference hospitals which could be explained due to the more complex and severe ophthalmological pathologies admitted in reference hospitals. In conclusion, Amniotic Membrane is used in a numerous ocular pathologies and especially on pterygium in our Mexican population.
authors.affiliation: Amniotic Membrane Bank, Cell and Tissue Biology, Research Unit, Instituto de Oftalmología Conde de Valenciana, Chimalpopoca 14, 06800, Mexico City, Mexico.
authors.forename: César
authors.initials: C
authors.lastname: Chávez-García
authors.affiliation: Department of Ocular Epidemiology, Instituto de Oftalmología Conde de Valenciana, Chimalpopoca 14, 06800, Mexico City, Mexico.
authors.forename: Aída
authors.initials: A
authors.lastname: Jiménez-Corona
authors.affiliation: Department of Cornea and Refractive Surgery, Instituto de Oftalmología Conde de Valenciana, Chimalpopoca 14, 06800, Mexico City, Mexico.
authors.forename: Enrique O
authors.initials: EO
authors.lastname: Graue-Hernández
authors.affiliation: Immunobiochemistry Department, Instituto Nacional de Perinatología Isidro Espinosa de los Reyes, Montes Urales 800, 11000, Mexico City, Mexico.
authors.forename: Verónica
authors.initials: V
authors.lastname: Zaga-Clavellina
authors.affiliation: Amniotic Membrane Bank, Cell and Tissue Biology, Research Unit, Instituto de Oftalmología Conde de Valenciana, Chimalpopoca 14, 06800, Mexico City, Mexico.
authors.forename: Mariana
authors.initials: M
authors.lastname: García-Mejía
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Universidad Nacional Autónoma de México, Avenida Universidad 3000, 04510, Mexico City, Mexico.
authors.forename: María Carmen
authors.initials: MC
authors.lastname: Jiménez-Martínez
authors.affiliation: Amniotic Membrane Bank, Cell and Tissue Biology, Research Unit, Instituto de Oftalmología Conde de Valenciana, Chimalpopoca 14, 06800, Mexico City, Mexico. yogarfias@institutodeoftalmologia.org.|Department of Biochemistry, Faculty of Medicine, Universidad Nacional Autónoma de México, Avenida Universidad 3000, 04510, Mexico City, Mexico. yogarfias@institutodeoftalmologia.org.
authors.forename: Yonathan
authors.initials: Y
authors.lastname: Garfias
mesh_terms: D000650:Amnion / Q000637:transplantation; D005260:Female; D006801:Humans; D008297:Male; D008800:Mexico; D008875:Middle Aged; D009885:Ophthalmology / Q000379:methods*; D014015:Tissue Banks
keywords: Amniotic Membrane Bank; Mexico; Ophthalmic indications; Pterygium