title: Host-Seeking Behavior and Arbovirus Detection in Mosquitoes of Habahe County, Xinjiang Uigur Autonomous Region, China.
abstract: Mosquitoes in Habahe County of Xinjiang Uigur Autonomous Region in China are considered a serious nuisance problem to local residents, but little is known of their role in enzootic disease. Therefore, host-seeking behavior and virus detection in mosquitoes were investigated in this study. Adult host-seeking mosquitoes were sampled using the Centers for Disease Control and Prevention (CDC) light traps operated at three locations in June through August 2008. Nine traps were used at each location at 3 different heights (1 m, 3 m, and 5 m). Seven mosquito species from 4 genera were collected by CDC light traps in different habitats. In total, 90,055 mosquitoes were captured, of which Aedes vexans was the most abundant species, comprising 88.02% of all mosquitoes collected. The second most abundant species was Anopheles messese, which comprised about 5.86%. Other species caught were Culex modestus (2.89%), Aedes caspius (1.11%), Coquillettidia richiardii (0.61%), Ae. dorsalis (1.36%), and An. hyrcanus (0.14%). About 93.5% of Ae. vexans individuals were caught in CO2-baited CDC light traps at 1 m above the ground. The highest numbers of Cx. modestus were caught at the highest trap level, 5 m above ground. Overall, significantly more mosquitoes of all species were collected at dusk than at dawn. Based on blood-meal analyses, Ae. vexans and An. messese fed on various vertebrate hosts, whereas Cx. modestus fed on ducks only. From a total of 335 mosquito pools tested, 10 pools of Ae. vexans were found positive for alphavirus. Comparison with the gene database revealed that the alphavirus deoxyribonucleic acid fragment obtained (GenBank accession no. HM160530) was 100% homologous at the nucleotide level to chikungunya virus isolate LK (PB) chik3408, chikungunya virus isolate SGEHICHD122508, and chikungunya virus strain FD080231. The results of this study suggest that ongoing, integrated mosquito and arbovirus surveillance is necessary in this river wetland.
authors.affiliation: 1  Department of Vector Biology and Control, State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Beijing 100071, China.
authors.forename: Xiao-Xia
authors.initials: XX
authors.lastname: Guo
authors.affiliation: 1  Department of Vector Biology and Control, State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Beijing 100071, China.
authors.forename: Ying-Mei
authors.initials: YM
authors.lastname: Zhang
authors.affiliation: 1  Department of Vector Biology and Control, State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Beijing 100071, China.
authors.forename: Chun-Xiao
authors.initials: CX
authors.lastname: Li
authors.affiliation: 2  Center for Disease Control and Prevention, Urumqi, Xinjiang 8300, China.
authors.forename: Gui-Lin
authors.initials: GL
authors.lastname: Zhang
authors.affiliation: 2  Center for Disease Control and Prevention, Urumqi, Xinjiang 8300, China.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Zheng
authors.affiliation: 1  Department of Vector Biology and Control, State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Beijing 100071, China.
authors.forename: Yan-De
authors.initials: YD
authors.lastname: Dong
authors.affiliation: 3  Anastasia Mosquito Control District, 500 Old Beach Road, St. Augustine, FL 32080, USA.
authors.forename: Rui-De
authors.initials: RD
authors.lastname: Xue
authors.affiliation: 1  Department of Vector Biology and Control, State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Beijing 100071, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Xing
authors.affiliation: 1  Department of Vector Biology and Control, State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Beijing 100071, China.
authors.forename: Tong-Yan
authors.initials: TY
authors.lastname: Zhao
mesh_terms: D000818:Animals; D001103:Arboviruses / Q000145:classification / Q000302:isolation & purification*; D002646:Chikungunya virus / Q000235:genetics / Q000302:isolation & purification*; D002681:China; D009033:Culicidae / Q000145:classification / Q000502:physiology* / Q000821:virology*; D005247:Feeding Behavior; D005260:Female; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000235:genetics
chemical_list: D014764:Viral Proteins
keywords: Aedes vexans; Xingjiang; arbovirus; blood meal; host-seeking