title: The Effects of Diabetes Mellitus on Ovarian Injury and Reserve: An Experimental Study.
abstract: OBJECTIVE
  The study aims to investigate the effects of diabetes mellitus (DM) on ovarian injury and reserve in a rat model.
  
  
  METHODS
  In this prospective experimental study, 16 female Sprague-Dawley albino rats (12 weeks, 220-240 g) were randomly divided into 2 groups. Group 1 included 8 normal healthy rats as controls. No drug was administered to the controls. Group 2 included the other 8 rats in which diabetes was induced by intraperitoneal injections of streptozotocin (STZ). After overt DM occurred (blood glucose >250 mg/dl), all the animals were euthanized and blood samples were collected by cardiac puncture for biochemical analysis. Bilateral oophorectomy was performed for histopathological examination. Immunoexpressions of nuclear factor-kappa B (NF-kB) and caspase-3 as well as anti-Müllerian hormone (AMH) levels were assessed. Values were analyzed by t test.
  
  
  RESULTS
  Immunoexpressions of NF-kB and caspase-3 were significantly higher in non-treated diabetic rats than in the control group (p = 0.011 and p = 0.010, respectively). In healthy control group, AMH levels (3.22 ± 0.58 ng/ml) were significantly higher than in the non-treated diabetic group (1.41 ± 0.25 ng/dl; p = 0.024).
  
  
  CONCLUSIONS
  Hyperglycemia causes severe ovarian injury via NF-kB pathway and caspase-3 apoptotic pathway, leading to the decrease in ovarian reserve in STZ-induced diabetic rats.
authors.affiliation: Department of Obstetrics and Gynecology, Erzincan University, School of Medicine, Erzincan, Turkey.
authors.forename: Umit
authors.initials: U
authors.lastname: Nayki
authors.forename: Didem
authors.initials: D
authors.lastname: Onk
authors.forename: Gurhan
authors.initials: G
authors.lastname: Balci
authors.forename: Cenk
authors.initials: C
authors.lastname: Nayki
authors.forename: Alper
authors.initials: A
authors.lastname: Onk
authors.forename: Murat
authors.initials: M
authors.lastname: Gunay
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology; D053148:Caspase 3 / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000150:complications / Q000503:physiopathology*; D004195:Disease Models, Animal; D005260:Female; D006943:Hyperglycemia / Q000150:complications / Q000503:physiopathology*; D016328:NF-kappa B / Q000378:metabolism; D065851:Ovarian Reserve / Q000502:physiology*; D010053:Ovary / Q000293:injuries* / Q000503:physiopathology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D016328:NF-kappa B; D053148:Caspase 3