title: Iodophenol blue-enhanced luminol chemiluminescence and its application to hydrogen peroxide and glucose detection.
abstract: In this study, we found that iodophenol blue can enhance the weak chemiluminescence (CL) of luminol-H2O2 system. With the aid of CL spectral, electron spin resonance (ESR) spectral measurements and studies on the effects of various free radical scavengers on the iodophenol blue-enhanced luminol-H2O2 system, we speculated that iodophenol blue may react with H2O2 and oxygen to produce oxidizing radical species such as OH(•) and O2(•-) resulting the formation of (1)O2. The generated (1)O2 may react with luminol anion generating an unstable endoperoxide and subsequent 3-aminophthalate* (3-APA*). When the excited-state 3-APA returned to the ground-state, an enhanced CL was observed. Based on the H2O2 concentration dependence of the catalytic activity of iodophenol blue, a cheap, simple, sensitive CL assay for the determination of H2O2 was established. Under the optimum experimental conditions, a linear relationship between the relative CL intensity and H2O2 concentration in the range of 0.025-10 μM was obtained. As low as 14 nM H2O2 can be sensitively detected by using the proposed method. The relative standard deviation for 5, 1 and 0.25 μM H2O2 was 2.58%, 5.16% and 4.66%, respectively. By combining the glucose oxidase (GOx)-catalyzed oxidation reaction, CL detection of glucose was realized. The linear range of glucose detection was 0.1-30 μM with a detection limit of 0.06 μM. The proposed method has been applied to the detection of glucose in diluted serum.
authors.affiliation: Tianjin Key Laboratory for Modern Drug Delivery & High-Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, People's Republic of China.
authors.forename: Dalong
authors.initials: D
authors.lastname: Yu
authors.affiliation: Tianjin Key Laboratory for Modern Drug Delivery & High-Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, People's Republic of China.
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.affiliation: Tianjin Key Laboratory for Modern Drug Delivery & High-Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, People's Republic of China.
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Tianjin Key Laboratory for Modern Drug Delivery & High-Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, People's Republic of China. Electronic address: aipingfan1980@163.com.
authors.forename: Aiping
authors.initials: A
authors.lastname: Fan
mesh_terms: D015374:Biosensing Techniques / Q000191:economics / Q000379:methods*; D005947:Glucose / Q000032:analysis* / Q000737:chemistry; D005949:Glucose Oxidase / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000032:analysis* / Q000737:chemistry; D057230:Limit of Detection; D008163:Luminescent Measurements; D008165:Luminol / Q000737:chemistry*; D010636:Phenols / Q000737:chemistry*
chemical_list: D010636:Phenols; C034202:iodophenol blue; D008165:Luminol; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D005947:Glucose
keywords: Chemiluminescence; Glucose; Hydrogen peroxide; Iodophenol blue; Luminol