title: Design of a Hyperpolarized Molecular Probe for Detection of Aminopeptidase N Activity.
abstract: Aminopeptidase N (APN) is an important enzyme that is involved in tumor angiogenesis. Detection of APN activity can thus lead to early diagnosis and elucidation of tumor development. Although some molecular probes for APN have been developed, the detection of APN activity in opaque biological samples remains a challenge. To this end, we designed a hyperpolarized NMR probe [1-(13) C]Ala-NH2 which satisfies the prerequisites for APN detection, namely, sufficient retention of the hyperpolarized state, a high reactivity to APN, and an APN-induced chemical shift change. The [1-(13) C]Ala-NH2 probe allowed sensitive detection of APN activity using (13) C NMR spectroscopy.
authors.affiliation: Department of Chemistry and Biochemistry, Graduate School of Engineering, Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka, 819-0395, Japan.
authors.forename: Ryunosuke
authors.initials: R
authors.lastname: Hata
authors.affiliation: Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8656, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nonaka
authors.affiliation: Incubation Center for Advanced Medical Science, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan.
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Takakusagi
authors.affiliation: Incubation Center for Advanced Medical Science, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan.|Innovation Center for Medical Redox Navigation, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Ichikawa
authors.affiliation: Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8656, Japan. ssando@chembio.t.u-tokyo.ac.jp.
authors.forename: Shinsuke
authors.initials: S
authors.lastname: Sando
mesh_terms: D018826:CD13 Antigens / Q000378:metabolism*; D007700:Kinetics; D015335:Molecular Probes / Q000737:chemistry*
chemical_list: D015335:Molecular Probes; D018826:CD13 Antigens
keywords: NMR spectroscopy; aminopeptidases; biosensors; dynamic nuclear polarization; hyperpolarization