title: Formation Ratios of Zearalanone, Zearalenols, and Zearalanols versus Zearalenone during Incubation of Fusarium semitectum on Sorghum and Ratios in Naturally Contaminated Sorghum.
abstract: We incubated Fusarium semitectum on sorghum and measured the production of zearalenone (ZEN) and ZEN-related compounds (zearalanone (ZAN), α-zearalenol (α-ZEL), β-zearalenol (β-ZEL), α-zearalanol (α-ZAL) and β-zearalanol (β-ZAL)) in the culture by LC-MS. Of the five ZEN-related compounds, ZAN and β-ZEL were mainly detected. The concentrations of ZEN and the five ZEN-related compounds increased until 9 days after incubation and then increased slightly or stayed constant between days 9 and 15. The ratios of α-ZEL, β-ZEL, α-ZAL and β-ZAL to ZEN decreased in a similar manner after 7 days, whereas the ratio of ZAN to ZEN remained constant after 5 days. Analysis of naturally contaminated sorghum by LC-MS/MS revealed that the production ratio of α-ZEL to ZEN was inconsistent with that of our in vitro incubation analysis. The results indicate that ZAN might not be suitable for use as an internal standard.
authors.affiliation: Food and Agricultural Materials Inspection Center.
authors.forename: Koji
authors.initials: K
authors.lastname: Aoyama
authors.forename: Eiichi
authors.initials: E
authors.lastname: Ishikuro
authors.forename: Hiroko
authors.initials: H
authors.lastname: Noriduki
authors.forename: Masakatsu
authors.initials: M
authors.lastname: Ichinoe
mesh_terms: D002853:Chromatography, Liquid; D005506:Food Contamination; D005670:Fusarium / Q000378:metabolism*; D013058:Mass Spectrometry; D045868:Sorghum / Q000378:metabolism* / Q000382:microbiology*; D053719:Tandem Mass Spectrometry; D015025:Zearalenone / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism*; D015029:Zeranol / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism*
chemical_list: C029659:zearalenol; D015025:Zearalenone; D015029:Zeranol