title: Novel Routes for Improving Biocontrol Activity of Bacillus Based Bioinoculants.
abstract: Biocontrol (BC) formulations prepared from plant-growth-promoting bacteria are increasingly applied in sustainable agriculture. Especially inoculants prepared from endospore-forming Bacillus strains have been proven as efficient and environmental-friendly alternative to chemical pesticides due to their long shelf life, which is comparable with that of agrochemicals. However, these formulations of the first generation are sometimes hampered in their action and do not fulfill in each case the expectations of the appliers. In this review we use the well-known plant-associated Bacillus amyloliquefaciens type strain FZB42 as example for the successful application of different techniques offered today by comparative, evolutionary and functional genomics, site-directed mutagenesis and strain construction including marker removal, for paving the way for preparing a novel generation of BC agents.
authors.affiliation: College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Monitoring and Management of Crop Disease and Pest Insects, Ministry of Agriculture Nanjing, China.
authors.forename: Liming
authors.initials: L
authors.lastname: Wu
authors.affiliation: College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Monitoring and Management of Crop Disease and Pest Insects, Ministry of Agriculture Nanjing, China.
authors.forename: Hui-Jun
authors.initials: HJ
authors.lastname: Wu
authors.affiliation: College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Monitoring and Management of Crop Disease and Pest Insects, Ministry of Agriculture Nanjing, China ; Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences Nanjing, China.
authors.forename: Junqing
authors.initials: J
authors.lastname: Qiao
authors.affiliation: College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Monitoring and Management of Crop Disease and Pest Insects, Ministry of Agriculture Nanjing, China.
authors.forename: Xuewen
authors.initials: X
authors.lastname: Gao
authors.affiliation: Fachgebiet Phytomedizin, Institut für Agrar- und Gartenbauwissenschaften, Humboldt-Universität zu Berlin Berlin, Germany ; Nord Reet UG Greifswald, Germany.
authors.forename: Rainer
authors.initials: R
authors.lastname: Borriss
keywords: Bacillus amyloliquefaciens subsp. plantarum; bacillomycin D; bacilysin; biocontrol; harpin genes; mersacidin; plant growth-promotion; surfactin