title: Crystallization of P-type ATPases by the High Lipid-Detergent (HiLiDe) Method.
abstract: Determining structures of membrane proteins remains a significant challenge. A technique utilizing high lipid-detergent concentrations ("HiLiDe") circumvents the major bottlenecks of current membrane protein crystallization methods. During HiLiDe, the protein-lipid-detergent ratio is varied in a controlled way in order to yield initial crystal hits, which may be subsequently optimized by variation of the crystallization conditions and/or utilizing secondary detergents. HiLiDe preserves the advantages of classical lipid-based methods, yet is compatible with both the vapor diffusion and batch crystallization techniques. The method has been applied with particular success to P-type ATPases.
authors.affiliation: Department of Molecular Biology and Genetics, Aarhus University, Aarhus C, Denmark.
authors.forename: Oleg
authors.initials: O
authors.lastname: Sitsel
authors.affiliation: Department of Biomedical Sciences, University of Copenhagen, Copenhagen, Germany.
authors.forename: Kaituo
authors.initials: K
authors.lastname: Wang
authors.affiliation: School of Medicine, Tsinghua University, Beijing, China.
authors.forename: Xiangyu
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Biomedical Sciences, University of Copenhagen, Copenhagen, Germany.|Department of Experimental Medical Science, Lund University, Sölvegatan 19, SE-22184, Lund, Sweden.
authors.forename: Pontus
authors.initials: P
authors.lastname: Gourdon
mesh_terms: D000251:Adenosine Triphosphatases / Q000737:chemistry*; D015925:Cryopreservation; D003460:Crystallization / Q000379:methods*; D003902:Detergents / Q000737:chemistry*; D008055:Lipids / Q000737:chemistry*
chemical_list: D003902:Detergents; D008055:Lipids; D000251:Adenosine Triphosphatases
keywords: Crystal; HiLiDe; Membrane proteins; P-type ATPase; Structure; X-ray diffraction