title: Reduction of Salmonella on chicken breast fillets stored under aerobic or modified atmosphere packaging by the application of lytic bacteriophage preparation SalmoFreshTM.
abstract: The present study evaluated the efficacy of recently approved Salmonella lytic bacteriophage preparation (SalmoFresh™) in reducing Salmonella on chicken breast fillets, as a surface and dip application. The effectiveness of phage in combination with modified atmosphere packaging (MAP) and the ability of phage preparation in reducing Salmonella on chicken breast fillets at room temperature was also evaluated. Chicken breast fillets inoculated with a cocktail of Salmonella Typhimurium, S. Heidelberg, and S. Enteritidis were treated with bacteriophage (10(9) PFU/mL) as either a dip or surface treatment. The dip-treated samples were stored at 4°C aerobically and the surface-treated samples were stored under aerobic and MAP conditions (95% CO2/5% O2) at 4°C for 7 d. Immersion of Salmonella-inoculated chicken breast fillets in bacteriophage solution reduced Salmonella (P < 0.05) by 0.7 and 0.9 log CFU/g on d 0 and d 1 of storage, respectively. Surface treatment with phage significantly (P < 0.05) reduced Salmonella by 0.8, 0.8, and 1 log CFU/g on d 0, 1, and 7 of storage, respectively, under aerobic conditions. Higher reductions in Salmonella counts were achieved on chicken breast fillets when the samples were surface treated with phage and stored under MAP conditions. The Salmonella counts were reduced by 1.2, 1.1, and 1.2 log CFU/g on d 0, 1, and 7 of storage, respectively. Bacteriophage surface application on chicken breast fillets stored at room temperature reduced the Salmonella counts by 0.8, 0.9, and 0.4 log CFU/g after 0, 4, and 8 h, respectively, compared to the untreated positive control. These findings indicate that lytic phage preparation was effective in reducing Salmonella on chicken breast fillets stored under aerobic and modified atmosphere conditions.
authors.affiliation: Poultry Science Department, Mississippi State University, Mississippi State.
authors.forename: Anuraj T
authors.initials: AT
authors.lastname: Sukumaran
authors.affiliation: Department of Food Science, Nutrition and Health Promotion, Mississippi State University, Mississippi State.
authors.forename: Rama
authors.initials: R
authors.lastname: Nannapaneni
authors.affiliation: Poultry Science Department, Mississippi State University, Mississippi State.
authors.forename: Aaron
authors.initials: A
authors.lastname: Kiess
authors.affiliation: Poultry Science Department, Mississippi State University, Mississippi State csharma@poultry.msstate.edu.
authors.forename: Chander Shekhar
authors.initials: CS
authors.lastname: Sharma
mesh_terms: D000818:Animals; D002645:Chickens; D005506:Food Contamination / Q000517:prevention & control*; D005516:Food Microbiology; D018857:Food Packaging / Q000592:standards*; D008460:Meat / Q000032:analysis / Q000382:microbiology*; D010369:Pectoralis Muscles / Q000382:microbiology* / Q000502:physiology; D012483:Salmonella Phages / Q000502:physiology*; D019779:Salmonella enterica / Q000821:virology*
keywords: Salmonella; bacteriophage; chicken; modified atmosphere packaging