title: Using aptamers to elucidate esophageal cancer clinical samples.
abstract: The epithelial cell adhesion molecule (EpCAM) is closely correlated with the occurrence and development of various cancers of epithelial origin. This study tested, for the first time, the ability of EpCAM aptamer SYL3C to detect EpCAM expression in 170 cases of esophageal cancer (EC) and precancerous lesions, as well as 20 cases of EC series samples, using immunofluorescence imaging analysis. Corresponding antibodies were used as control. EpCAM overexpression was 98% in both esophageal squamous cell carcinoma (ESCC) and esophageal adenocarcinoma (EACA) and 100% in metastasis, but no EpCAM overexpression was detected in undifferentiated EC (UEC). Significant differences were noted among various stages of differentiation (p < 0.05) with the degree of differentiation inversely correlated with the expression of EpCAM. Overexpressed EpCAM was detected in severe dysplasia, but negative in mild to moderate dysplasia and benign esophageal lesions. In a competitive binding experiment, EpCAM aptamer generated a staining pattern similar to that of antibody, but the binding sites with EpCAM were different. Based on these results, it can be concluded that EpCAM is suitable for use as an EC biomarker, therapeutic target, and effective parameter for tumor transfer and prognosis evaluation by aptamer SYL3C staining.
authors.affiliation: Xiangya Hospital, Central South University, Changsha, Hunan 410008, China.
authors.forename: Zhenxu
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Xiangya Hospital, Central South University, Changsha, Hunan 410008, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Xiangya Hospital, Central South University, Changsha, Hunan 410008, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Pu
authors.affiliation: Xiangya Hospital, Central South University, Changsha, Hunan 410008, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.affiliation: Xiangya Hospital, Central South University, Changsha, Hunan 410008, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Liu
authors.affiliation: Xiangya Hospital, Central South University, Changsha, Hunan 410008, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Yu
authors.affiliation: Xiangya Hospital, Central South University, Changsha, Hunan 410008, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Chen
authors.affiliation: Molecular Science and Biomedicine Laboratory, State Key Laboratory for Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, and Collaborative Research Center of Molecular Engineering for Theranostics, Hunan University, Changsha, Hunan 410082, China.|Department of Chemistry and Physiology and Functional Genomics, Center for Research at the Bio/Nano Interface, University Health Cancer Center, University of Florida, Gainesville, Florida 32611-7200, United States.
authors.forename: Ting
authors.initials: T
authors.lastname: Fu
authors.affiliation: State Key Laboratory for Physical Chemistry of Solid Surfaces, Key Laboratory for Chemical Biology of Fujian Province, Key Laboratory of Analytical Chemistry, and Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, China.|Department of Chemistry and Physiology and Functional Genomics, Center for Research at the Bio/Nano Interface, University Health Cancer Center, University of Florida, Gainesville, Florida 32611-7200, United States.
authors.forename: Chaoyong James
authors.initials: CJ
authors.lastname: Yang
authors.affiliation: Xiangya Hospital, Central South University, Changsha, Hunan 410008, China.
authors.forename: Huixia
authors.initials: H
authors.lastname: Liu
authors.affiliation: Molecular Science and Biomedicine Laboratory, State Key Laboratory for Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, and Collaborative Research Center of Molecular Engineering for Theranostics, Hunan University, Changsha, Hunan 410082, China.|Department of Chemistry and Physiology and Functional Genomics, Center for Research at the Bio/Nano Interface, University Health Cancer Center, University of Florida, Gainesville, Florida 32611-7200, United States.
authors.forename: Weihong
authors.initials: W
authors.lastname: Tan
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D014408:Biomarkers, Tumor / Q000096:biosynthesis* / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D000071858:Epithelial Cell Adhesion Molecule / Q000096:biosynthesis* / Q000235:genetics; D004938:Esophageal Neoplasms / Q000235:genetics* / Q000473:pathology; D000077277:Esophageal Squamous Cell Carcinoma; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008207:Lymphatic Metastasis; D009367:Neoplasm Staging; D011379:Prognosis; D052156:SELEX Aptamer Technique / Q000379:methods
chemical_list: D014408:Biomarkers, Tumor; C499379:EPCAM protein, human; D000071858:Epithelial Cell Adhesion Molecule