title: Resveratrol Protects PC12 Cell against 6-OHDA Damage via CXCR4 Signaling Pathway.
abstract: Resveratrol, herbal nonflavonoid polyphenolic compound naturally derived from grapes, has long been acknowledged to possess extensive biological and pharmacological properties including antioxidant and anti-inflammatory ones and may exert a neuroprotective effect on neuronal damage in neurodegenerative diseases. However, the underlying molecular mechanisms remain undefined. In the present study, we intended to investigate the neuroprotective effects of resveratrol against 6-OHDA-induced neurotoxicity of PC12 cells and further explore the possible mechanisms involved. For this purpose, PC12 cells were exposed to 6-OHDA in the presence of resveratrol (0, 12.5, 25, and 50 μM). The results showed that resveratrol increased cell viability, alleviated the MMP reduction, and reduced the number of apoptotic cells as measured by MTT assay, JC-1 staining, and Hoechst/PI double staining (all p < 0.01). Immunofluorescent staining and Western blotting revealed that resveratrol averts 6-OHDA induced CXCR4 upregulation (p < 0.01). Our results demonstrated that resveratrol could effectively protect PC12 cells from 6-OHDA-induced oxidative stress and apoptosis via CXCR4 signaling pathway.
authors.affiliation: Department of Anatomy and Key Laboratory of The Ministry of Education for Experimental Teratology, School of Medicine, Shandong University, Jinan, Shandong 250012, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Traumatic Orthopaedics, Shandong Provincial Hospital, Shandong University, Jinan, Shandong 250012, China.
authors.forename: Wenchuang
authors.initials: W
authors.lastname: Fan
authors.affiliation: Department of Anatomy and Key Laboratory of The Ministry of Education for Experimental Teratology, School of Medicine, Shandong University, Jinan, Shandong 250012, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Anatomy and Key Laboratory of The Ministry of Education for Experimental Teratology, School of Medicine, Shandong University, Jinan, Shandong 250012, China.
authors.forename: Lihua
authors.initials: L
authors.lastname: Bao
authors.affiliation: Department of Anatomy and Key Laboratory of The Ministry of Education for Experimental Teratology, School of Medicine, Shandong University, Jinan, Shandong 250012, China.
authors.forename: Guibao
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Neurosurgery, The Fourth Hospital of Jinan City, Jinan, Shandong 250012, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Li
authors.affiliation: Department of Anatomy and Key Laboratory of The Ministry of Education for Experimental Teratology, School of Medicine, Shandong University, Jinan, Shandong 250012, China.
authors.forename: Shouyang
authors.initials: S
authors.lastname: Song
authors.affiliation: Department of Anatomy and Key Laboratory of The Ministry of Education for Experimental Teratology, School of Medicine, Shandong University, Jinan, Shandong 250012, China.
authors.forename: Hongyu
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Histology and Embryology, School of Medicine, Shandong University, Jinan, Shandong 250012, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Hao
authors.affiliation: Department of Anatomy and Key Laboratory of The Ministry of Education for Experimental Teratology, School of Medicine, Shandong University, Jinan, Shandong 250012, China.
authors.forename: Jinhao
authors.initials: J
authors.lastname: Sun