title: Sarcocystis tuagulusi, n.sp. (Apicomplexa: Sarcocystidae) from Williamson's mouse deer (Tuagulus williamsoni) (Artiodactyla:Tragulidae).
abstract: Numerous species of Sarcocystis have been reported from wild ruminants, but none has been from Williamson's mouse deer (Tuagulus williamsoni). Here, we describe a new species, Sarcocystis tuagulusi, infecting five of 12 Williamson's mouse deer from southwest China. Mature sarcocysts are microscopic, up to 2280-μm long. The sarcocyst wall had 8.2-μm long villar protrusions, type 24. Using transmission electron microscopy, the protrusions on sarcocysts appeared as elongated, lancet- or leaf-like shapes in longitudinal sections; in contrast, the cross-sections revealed mushroom-shaped protrusions. In the core of the protrusion, a bundle of microtubules penetrated diagonally into a ground substance along the longitudinal axis. A phyogenetic analysis based on 18S rRNA and cox1 sequences suggested S. tuagulusi is closely related to Sarcocystis species from ruminants that employ felids as definitive hosts.
authors.affiliation: School of Biological Sciences, Yunnan University, Kunming, 650091, China. jjhu@ynu.edu.cn.
authors.forename: Jun Jie
authors.initials: JJ
authors.lastname: Hu
authors.affiliation: School of Biological Sciences, Yunnan University, Kunming, 650091, China.
authors.forename: Si
authors.initials: S
authors.lastname: Huang
authors.affiliation: School of Biological Sciences, Yunnan University, Kunming, 650091, China.
authors.forename: Ming Yong
authors.initials: MY
authors.lastname: Chen
authors.affiliation: School of Biological Sciences, Yunnan University, Kunming, 650091, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Wen
authors.affiliation: Department of Biology, Wake Forest University, Winston-Salem, NC, 27106, USA.
authors.forename: Gerald Wisler
authors.initials: GW
authors.lastname: Esch
authors.affiliation: School of Biological Sciences, Yunnan University, Kunming, 650091, China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Liu
authors.affiliation: School of Biological Sciences, Yunnan University, Kunming, 650091, China.
authors.forename: Ting Ting
authors.initials: TT
authors.lastname: Liu
mesh_terms: D000818:Animals; D002681:China / Q000453:epidemiology; D003670:Deer; D015971:Gene Expression Regulation, Enzymologic; D051379:Mice; D046529:Microscopy, Electron, Transmission; D010802:Phylogeny; D016053:RNA, Protozoan / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D012522:Sarcocystis / Q000145:classification / Q000302:isolation & purification*; D012523:Sarcocystosis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*
chemical_list: D016053:RNA, Protozoan; D012337:RNA, Ribosomal, 18S
keywords: Phylogeney; Sarcocystis; Tuagulus williamsoni; Ultrastrucure; Williamson’s mouse deer