title: Quinic acid derivatives inhibit dengue virus replication in vitro.
abstract: BACKGROUND
  Dengue is the most prevalent arboviral disease in tropical and sub-tropical areas of the world. The incidence of infection is estimated to be 390 million cases and 25,000 deaths per year. Despite these numbers, neither a specific treatment nor a preventive vaccine is available to protect people living in areas of high risk.
  
  
  RESULTS
  With the aim of seeking a treatment that can mitigate dengue infection, we demonstrated that the quinic acid derivatives known as compound 2 and compound 10 were effective against all four dengue virus serotypes and safe for use in a human hepatoma cell line (Huh7.5). Both compounds were non-virucidal to dengue virus particles and did not interfere with early steps of the dengue virus life cycle, including binding and internalization. Experiments using a replicon system demonstrated that compounds 2 and 10 impaired dengue virus replication in Huh7.5 cells. Additionally, the anti-dengue virus effects of the quinic acid derivatives were preserved in human peripheral blood mononuclear cells.
  
  
  CONCLUSIONS
  Taken together, these data suggest that quinic acid derivatives represent a novel chemical class of active compounds that could be used to combat dengue virus infection.
authors.affiliation: Laboratório de Virologia Molecular, Instituto Carlos Chagas, ICC/Fiocruz, 81350-010, Curitiba, PR, Brazil. paula.zanello@hotmail.com.
authors.forename: Paula Rodrigues
authors.initials: PR
authors.lastname: Zanello
authors.affiliation: Laboratório de Virologia Molecular, Instituto Carlos Chagas, ICC/Fiocruz, 81350-010, Curitiba, PR, Brazil. dea_koishi@yahoo.com.br.
authors.forename: Andrea Cristine
authors.initials: AC
authors.lastname: Koishi
authors.affiliation: Departamento de Química, Universidade Federal de Juiz de Fora, 36036-330, Juiz de Fora, MG, Brazil. celso_quimica@yahoo.com.br.
authors.forename: Celso de Oliveira
authors.initials: Cde O
authors.lastname: Rezende Júnior
authors.affiliation: Departamento de Química, Universidade Federal de Juiz de Fora, 36036-330, Juiz de Fora, MG, Brazil. larissa.jf@hotmail.com.
authors.forename: Larissa Albuquerque
authors.initials: LA
authors.lastname: Oliveira
authors.affiliation: Departamento de Química, Universidade Federal de Juiz de Fora, 36036-330, Juiz de Fora, MG, Brazil. adrianeapquimica@hotmail.com.
authors.forename: Adriane Antonia
authors.initials: AA
authors.lastname: Pereira
authors.affiliation: Departamento de Química, Universidade Federal de Juiz de Fora, 36036-330, Juiz de Fora, MG, Brazil. mauro.almeida@ufjf.edu.br.
authors.forename: Mauro Vieira
authors.initials: MV
authors.lastname: de Almeida
authors.affiliation: Laboratório de Virologia Molecular, Instituto Carlos Chagas, ICC/Fiocruz, 81350-010, Curitiba, PR, Brazil. clsantos@tecpar.br.
authors.forename: Claudia Nunes
authors.initials: CN
authors.lastname: Duarte dos Santos
authors.affiliation: Laboratório de Virologia Molecular, Instituto Carlos Chagas, ICC/Fiocruz, 81350-010, Curitiba, PR, Brazil. bordignonjuliano@gmail.com.
authors.forename: Juliano
authors.initials: J
authors.lastname: Bordignon
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D009033:Culicidae; D003716:Dengue Virus / Q000187:drug effects* / Q000502:physiology*; D022781:Hepatocytes / Q000187:drug effects; D006801:Humans; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability / Q000187:drug effects; D008958:Models, Molecular; D015394:Molecular Structure; D011801:Quinic Acid / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D014779:Virus Replication / Q000187:drug effects*
chemical_list: D000998:Antiviral Agents; D011801:Quinic Acid