title: The Identification of Two Head Smut Resistance-Related QTL in Maize by the Joint Approach of Linkage Mapping and Association Analysis.
abstract: Head smut, caused by the fungus Sphacelotheca reiliana (Kühn) Clint, is a devastating threat to maize production. In this study, QTL mapping of head smut resistance was performed using a recombinant inbred line (RIL) population from a cross between a resistant line "QI319" and a susceptible line "Huangzaosi" (HZS) with a genetic map constructed from genotyping-by-sequencing (GBS) data and composed of 1638 bin markers. Two head smut resistance QTL were identified, located on Chromosome 2 (q2.09HR) and Chromosome 5 (q5.03HR), q2.09HR is co-localized with a previously reported QTL for head smut resistance, and the effect of q5.03HR has been validated in backcross populations. It was also observed that pyramiding the resistant alleles of both QTL enhanced the level of resistance to head smut. A genome-wide association study (GWAS) using 277 diverse inbred lines was processed to validate the mapped QTL and to identify additional head smut resistance associations. A total of 58 associated SNPs were detected, which were distributed in 31 independent regions. SNPs with significant association to head smut resistance were detected within the q2.09HR and q5.03HR regions, confirming the linkage mapping results. It was also observed that both additive and epistastic effects determine the genetic architecture of head smut resistance in maize. As shown in this study, the combined strategy of linkage mapping and association analysis is a powerful approach in QTL dissection for disease resistance in maize.
authors.affiliation: Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
authors.forename: Yong-xiang
authors.initials: YX
authors.lastname: Li
authors.affiliation: Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
authors.forename: Xun
authors.initials: X
authors.lastname: Wu
authors.affiliation: DuPont Pioneer, Wilmington, DE, 19803, United States of America.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Jaqueth
authors.affiliation: Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
authors.forename: Dengfeng
authors.initials: D
authors.lastname: Zhang
authors.affiliation: DuPont Pioneer, Beijing 101309, China.
authors.forename: Donghui
authors.initials: D
authors.lastname: Cui
authors.affiliation: Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
authors.forename: Chunhui
authors.initials: C
authors.lastname: Li
authors.affiliation: Maize Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China.
authors.forename: Guanghui
authors.initials: G
authors.lastname: Hu
authors.affiliation: Institute of Plant Protection, Liaoning Academy of Agricultural Sciences, Shenyang 110161, China.
authors.forename: Huaiyu
authors.initials: H
authors.lastname: Dong
authors.affiliation: Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
authors.forename: Yan-chun
authors.initials: YC
authors.lastname: Song
authors.affiliation: Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
authors.forename: Yun-su
authors.initials: YS
authors.lastname: Shi
authors.affiliation: Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
authors.forename: Tianyu
authors.initials: T
authors.lastname: Wang
authors.affiliation: DuPont Pioneer, Wilmington, DE, 19803, United States of America.
authors.forename: Bailin
authors.initials: B
authors.lastname: Li
authors.affiliation: Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Li
mesh_terms: D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D060467:Disease Resistance / Q000235:genetics; D055106:Genome-Wide Association Study; D060005:Genotyping Techniques / Q000379:methods; D000069600:Plant Breeding; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology; D040641:Quantitative Trait Loci; D015203:Reproducibility of Results; D014587:Ustilaginales / Q000472:pathogenicity; D003313:Zea mays / Q000235:genetics* / Q000382:microbiology*