title: Arp2/3 complex subunit ARPC2 binds to microtubules.
abstract: Arp2/3 complex plays a fundamental role in the nucleation of actin filaments (AFs) in yeasts, plants, and animals. In plants, the aberrant shaping and elongation of several types of epidermal cells observed in Arp2/3 complex knockout plant mutants suggest the importance of Arp2/3-mediated actin nucleation for various morphogenetic processes. Here we show that ARPC2, a core Arp2/3 complex subunit, interacts with both actin filaments (AFs) and microtubules (MTs). Plant GFP-ARPC2 expressed in Nicotiana tabacum BY-2 cells, leaf epidermal cells of Nicotiana benthamiana and root epidermal cells of Arabidopsis thaliana decorated MTs. The interaction with MTs was demonstrated by pharmacological approach selectively interfering with either AFs or MTs dynamics as well as by the in vitro co-sedimentation assays. A putative MT-binding domain of tobacco NtARPC2 protein was identified using the co-sedimentation of several truncated NtARPC2 proteins with MTs. Newly identified MT-binding ability of ARPC2 subunit of Arp2/3 complex may represent a new molecular mechanism of AFs and MTs interaction.
authors.affiliation: Charles University in Prague, Faculty of Science, Department of Experimental Plant Biology, Viničná 5, 128 44 Prague 2, Czech Republic.
authors.forename: Lenka
authors.initials: L
authors.lastname: Havelková
authors.affiliation: Charles University in Prague, Faculty of Science, Department of Experimental Plant Biology, Viničná 5, 128 44 Prague 2, Czech Republic.
authors.forename: Gitanjali
authors.initials: G
authors.lastname: Nanda
authors.affiliation: Charles University in Prague, Faculty of Science, Department of Experimental Plant Biology, Viničná 5, 128 44 Prague 2, Czech Republic.
authors.forename: Jan
authors.initials: J
authors.lastname: Martinek
authors.affiliation: Charles University in Prague, Faculty of Science, Department of Experimental Plant Biology, Viničná 5, 128 44 Prague 2, Czech Republic.
authors.forename: Erica
authors.initials: E
authors.lastname: Bellinvia
authors.affiliation: Charles University in Prague, Faculty of Science, Department of Experimental Plant Biology, Viničná 5, 128 44 Prague 2, Czech Republic.
authors.forename: Lenka
authors.initials: L
authors.lastname: Sikorová
authors.affiliation: Charles University in Prague, Faculty of Science, Department of Experimental Plant Biology, Viničná 5, 128 44 Prague 2, Czech Republic.
authors.forename: Kateřina
authors.initials: K
authors.lastname: Šlajcherová
authors.affiliation: Charles University in Prague, Faculty of Science, Department of Experimental Plant Biology, Viničná 5, 128 44 Prague 2, Czech Republic.
authors.forename: Daniela
authors.initials: D
authors.lastname: Seifertová
authors.affiliation: Charles University in Prague, Faculty of Science, Department of Experimental Plant Biology, Viničná 5, 128 44 Prague 2, Czech Republic.
authors.forename: Lukáš
authors.initials: L
authors.lastname: Fischer
authors.affiliation: Charles University in Prague, Faculty of Science, Department of Experimental Plant Biology, Viničná 5, 128 44 Prague 2, Czech Republic.
authors.forename: Jindřiška
authors.initials: J
authors.lastname: Fišerová
authors.affiliation: Charles University in Prague, Faculty of Science, Department of Experimental Plant Biology, Viničná 5, 128 44 Prague 2, Czech Republic.
authors.forename: Jan
authors.initials: J
authors.lastname: Petrášek
authors.affiliation: Charles University in Prague, Faculty of Science, Department of Experimental Plant Biology, Viničná 5, 128 44 Prague 2, Czech Republic. Electronic address: schwarze@natur.cuni.cz.
authors.forename: Kateřina
authors.initials: K
authors.lastname: Schwarzerová
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism; D051376:Actin-Related Protein 2-3 Complex / Q000235:genetics* / Q000378:metabolism; D017360:Arabidopsis / Q000378:metabolism*; D008870:Microtubules / Q000378:metabolism; D018515:Plant Leaves / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D018517:Plant Roots / Q000378:metabolism; D014026:Nicotiana / Q000235:genetics* / Q000378:metabolism
chemical_list: D051376:Actin-Related Protein 2-3 Complex; D010940:Plant Proteins
keywords: Actin; Actin filaments; Arp2/3; Microtubules; Tubulin