title: Biomass and lipid enhancement in Chlorella sp. with emphasis on biodiesel quality assessment through detailed FAME signature.
abstract: In this study, the concentrations of MgSO4, salinity and light intensity were optimised for maximum biomass productivity and lipid content in Chlorella sp. Lipid synthesized at varied experimental conditions was also assessed in detail for biodiesel properties through FAME analysis. FAMEs mainly composed of C16:0, C16:1(9), C16:3(7, 10, 13), C18:0, C18:1(11), C18:2(9, 12), C18:3(9, 12, 15). The optimum biomass productivity (372.50mgL(-1)d(-1)) and lipid content (32.57%) was obtained at MgSO4-150ppm; salinity-12.5ppm, and light intensity-25μmolm(-2)s(-1). However, at this condition the cetane number, a major biodiesel property was not complying with worldwide biodiesel standard. Therefore, further optimisations were done to check the suitability of biodiesel fuel. The optimum biomass productivity (348.47mgL(-1)d(-1)) and lipid content (12.43%) with suitable biodiesel fuel properties was obtained at MgSO4-50ppm, salinity-25ppm and light intensity-100μmolm(-2)s(-1). The validation experiments confirmed the closeness of predicted and measured response values.
authors.affiliation: Environmental Health Division, CSIR-National Environmental Engineering Research Institute (NEERI), Nagpur, India.
authors.forename: Ajam Yakub
authors.initials: AY
authors.lastname: Shekh
authors.affiliation: Environmental Health Division, CSIR-National Environmental Engineering Research Institute (NEERI), Nagpur, India.
authors.forename: Preeti
authors.initials: P
authors.lastname: Shrivastava
authors.affiliation: Environmental Systems Design and Modeling Division, CSIR-National Environmental Engineering Research Institute (NEERI), Nagpur, India.
authors.forename: Ankit
authors.initials: A
authors.lastname: Gupta
authors.affiliation: Environmental Health Division, CSIR-National Environmental Engineering Research Institute (NEERI), Nagpur, India.
authors.forename: Kannan
authors.initials: K
authors.lastname: Krishnamurthi
authors.affiliation: Environmental Health Division, CSIR-National Environmental Engineering Research Institute (NEERI), Nagpur, India.
authors.forename: Sivanesan Saravana
authors.initials: SS
authors.lastname: Devi
authors.affiliation: Plant Cell Biotechnology Division, CSIR-Central Food Technological Research Institute, Mysore, India. Electronic address: sn.mudliar@gmail.com.
authors.forename: Sandeep N
authors.initials: SN
authors.lastname: Mudliar
mesh_terms: D000704:Analysis of Variance; D056804:Biofuels / Q000032:analysis*; D018533:Biomass; D002708:Chlorella / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D004952:Esters / Q000032:analysis*; D005227:Fatty Acids / Q000032:analysis*; D008027:Light; D008278:Magnesium Sulfate / Q000494:pharmacology; D012044:Regression Analysis; D054712:Salinity
chemical_list: D056804:Biofuels; D004952:Esters; D005227:Fatty Acids; D008278:Magnesium Sulfate
keywords: Biodiesel properties; Chlorella sp.; Light intensity; MgSO(4); Salinity