title: Dog Tear Film Proteome In-Depth Analysis.
abstract: In this study, mass spectrometry was used to explore the canine tear proteome. Tear samples were obtained from six healthy dogs, and one-dimensional sodium dodecyl sulphate polyacrylamide gel electrophoresis (1D SDS-PAGE) was used as a first step to separate intact proteins into 17 bands. Each fraction was then trypsin digested and analysed by matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry (MALDI-TOF-MS/MS) to characterize the protein components in each fraction. In total, 125 tear proteins were identified, with MCA (Major Canine Allergen), Serum albumin, UPF0557 protein C10orf119 homolog, Collagen alpha-2(I) chain, Tyrosine -protein kinase Fer, Keratine type II cytoskeletal, Beta-crystallin B2, Interleukin-6 and Desmin occurring as the most confident ones with the highest scores. The results showed that the proteomic strategy used in this study was successful in the analysis of the dog tear proteome. To the best of our knowledge, this study is the first to report the comprehensive proteome profile of tears from healthy dogs by 1D SDS PAGE and MALDI-TOF. Data are available via ProteomeXchange with identifier PXD003124.
authors.affiliation: Department of Vitreoretinal Surgery, Medical University of Lublin, 20-079 Lublin, Chmielna 1, Poland.
authors.forename: Mateusz
authors.initials: M
authors.lastname: Winiarczyk
authors.affiliation: Department and Clinic of Animal Internal Diseases, University of Life Sciences, 20-612 Lublin, Głęboka 30, Poland.
authors.forename: Dagmara
authors.initials: D
authors.lastname: Winiarczyk
authors.affiliation: Department of Epizootiology and Clinic of Infectious Diseases, University of Life Sciences, 20-612 Lublin, Głęboka 30, Poland.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Banach
authors.affiliation: Department of Epizootiology and Clinic of Infectious Diseases, University of Life Sciences, 20-612 Lublin, Głęboka 30, Poland.
authors.forename: Lukasz
authors.initials: L
authors.lastname: Adaszek
authors.affiliation: Department and Clinic of Animal Internal Diseases, University of Life Sciences, 20-612 Lublin, Głęboka 30, Poland.
authors.forename: Jacek
authors.initials: J
authors.lastname: Madany
authors.affiliation: Department of Vitreoretinal Surgery, Medical University of Lublin, 20-079 Lublin, Chmielna 1, Poland.
authors.forename: Jerzy
authors.initials: J
authors.lastname: Mackiewicz
authors.affiliation: Department of Epizootiology and Clinic of Infectious Diseases, University of Life Sciences, 20-612 Lublin, Głęboka 30, Poland.
authors.forename: Dorota
authors.initials: D
authors.lastname: Pietras-Ozga
authors.affiliation: Department of Epizootiology and Clinic of Infectious Diseases, University of Life Sciences, 20-612 Lublin, Głęboka 30, Poland.
authors.forename: Stanislaw
authors.initials: S
authors.lastname: Winiarczyk
mesh_terms: D000818:Animals; D004285:Dogs; D004591:Electrophoresis, Polyacrylamide Gel; D011506:Proteins / Q000737:chemistry / Q000145:classification / Q000378:metabolism; D020543:Proteome; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013666:Tears / Q000378:metabolism*
chemical_list: D011506:Proteins; D020543:Proteome