title: Proteomic Profiling of Human Small Cell Lung Cancer Cell Line NCI-H211.
abstract: OBJECTIVE
  Small cell lung cancer is one of the major causes of death from cancer worldwide. To explore the expressions of global protein in small cell lung cancer cells, a proteomic approach, to identify the proteins, was used and the establishment of a protein reference map attempted.
  
  
  METHODS
  Two-dimensional gel electrophoresis (2-DE), with subsequent analysis by mass spectrometry (MS), was applied to the study of protein identification from a small cell lung cancer cell line, NCI- H211. The cells were lysed, and the extracts subjected to isoelectric focusing, with immobilized pH gradients, followed by second dimension SDS-PAGE. The polypeptides were identified by peptide mass fingerprinting, with MALDI-TOF MS, after in-gel protein digestion.
  
  
  RESULTS
  From silver staining of the gel, around two thousands protein spots were separated by the 2-DE. Of these protein spots visualized in the gel, one hundred and ten were identified by peptide mass fingerprinting. Different proteins, such as enzymes, cytoskeletal proteins and proteins common to eukaryotic cells, were identified.
  
  
  CONCLUSIONS
  The protein expressions of the small cell lung cancer cells were analyzed to establish a protein reference map. The reference map presented here may serve as a working tool for the further study of small cell lung cancer.
authors.forename: Hee Young
authors.initials: HY
authors.lastname: Cho
authors.forename: Mi Kyung
authors.initials: MK
authors.lastname: Kim
authors.forename: Young Do
authors.initials: YD
authors.lastname: Yoo
authors.forename: Myung Ju
authors.initials: MJ
authors.lastname: Ahn
authors.forename: Joung Soon
authors.initials: JS
authors.lastname: Jang
keywords: Proteome; Reference map; Small cell lung cancer