title: Alternative splicing transcription of Megalobrama amblycephala HIF prolyl hydroxylase PHD3 and up-regulation of PHD3 by HIF-1α.
abstract: PHD3 is a hydroxylase that hydroxylates prolyl residues on hypoxia-inducible factors (HIFs) in mammals. In this study, the full-length cDNA and promoter sequences of Megalobrama amblycephala PHD3 gene were isolated by a modified RACE method. PHD3 cDNA was 1622 bp in length, including an ORF of 717 bp encoding 238 amino acid residues. The semi-quantitative PCR results suggested that PHD3 was highly expressed in liver in the normal condition, while after hypoxia treatment this gene was significantly increased in all analyzed tissues. PHD3 was detected only in the initial stages of M. amblycephala embryo development. In addition, the presence of another alternatively processed PHD3 transcript, designated PHD3Δ1 was observed in the process of analyzing the expression of PHD3. Both PHD3 and PHD3Δ1 were up-regulated under hypoxia, and had five the hypoxia response elements (HREs) by in silico scanning on the promoter. Further luciferase assay indicated that all HREs significantly responded to hypoxia. Taken together, these results suggest that PHD3 plays important roles in hypoxia response and early embryo development of M. amblycephala.
authors.affiliation: Key Lab of Freshwater Animal Breeding, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Fishery, Huazhong Agricultural University, 430070, Wuhan, PR China.
authors.forename: Nan
authors.initials: N
authors.lastname: Chen
authors.affiliation: Key Lab of Freshwater Animal Breeding, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Fishery, Huazhong Agricultural University, 430070, Wuhan, PR China.
authors.forename: Cui-Hong
authors.initials: CH
authors.lastname: Huang
authors.affiliation: Key Lab of Freshwater Animal Breeding, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Fishery, Huazhong Agricultural University, 430070, Wuhan, PR China; Hubei BaiRong Improved Aquatic Seed Co., Ltd, 438800 Huanggang, PR China.
authors.forename: Bo-Xiang
authors.initials: BX
authors.lastname: Chen
authors.affiliation: Key Lab of Freshwater Animal Breeding, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Fishery, Huazhong Agricultural University, 430070, Wuhan, PR China; Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, 430070, Wuhan, PR China.
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.affiliation: Key Lab of Freshwater Animal Breeding, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Fishery, Huazhong Agricultural University, 430070, Wuhan, PR China.
authors.forename: Wei-Min
authors.initials: WM
authors.lastname: Wang
authors.affiliation: Department of Zoology, Government College Women University Faisalabad, Pakistan.
authors.forename: Yasmeen
authors.initials: Y
authors.lastname: Gul
authors.affiliation: Key Lab of Freshwater Animal Breeding, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Fishery, Huazhong Agricultural University, 430070, Wuhan, PR China; Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, 430070, Wuhan, PR China. Electronic address: hbauwhl@hotmail.com.
authors.forename: Huan-Ling
authors.initials: HL
authors.lastname: Wang
mesh_terms: D017398:Alternative Splicing / Q000235:genetics*; D000818:Animals; D047108:Embryonic Development / Q000235:genetics*; D005399:Fishes / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental / Q000235:genetics; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics*; D064799:Hypoxia-Inducible Factor-Proline Dioxygenases / Q000235:genetics*; D015533:Transcriptional Activation / Q000235:genetics*; D015854:Up-Regulation / Q000235:genetics
chemical_list: C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D064799:Hypoxia-Inducible Factor-Proline Dioxygenases
keywords: Alternative splicing transcription; Hypoxia; Megalobrama amblycephala; PHD3