title: Metabolomic profiling from leaves and roots of tomato (Solanum lycopersicum L.) plants grown under nitrogen, phosphorus or potassium-deficient condition.
abstract: Specific metabolic network responses to mineral deficiencies are not well-defined. Here, we conducted a detailed broad-scale identification of metabolic responses of tomato leaves and roots to N, P or K deficiency. Tomato plants were grown hydroponically under optimal (5mM N, 0.5mM P, or 5mM K) and deficient (0.5mM N, 0.05mM P, or 0.5mM K) conditions and metabolites were measured by LC-MS and GC-MS. Based on these results, deficiency of any of these three minerals affected energy production and amino acid metabolism. N deficiency generally led to decreased amino acids and organic acids, and increased soluble sugars. P deficiency resulted in increased amino acids and organic acids in roots, and decreased soluble sugars. K deficiency caused accumulation of soluble sugars and amino acids in roots, and decreased organic acids and amino acids in leaves. Notable metabolic pathway alterations included; (1) increased levels of α-ketoglutarate and raffinose family oligosaccharides in N, P or K-deficient tomato roots, and (2) increased putrescine in K-deficient roots. These findings provide new knowledge of metabolic changes in response to mineral deficiencies.
authors.affiliation: Division of Soil and Fertilizer, National Academy of Agricultural Science, RDA, Wanju, North Jeolla Province 565-851, Republic of Korea.
authors.forename: Jwakyung
authors.initials: J
authors.lastname: Sung
authors.affiliation: Division of Soil and Fertilizer, National Academy of Agricultural Science, RDA, Wanju, North Jeolla Province 565-851, Republic of Korea.
authors.forename: Suyeon
authors.initials: S
authors.lastname: Lee
authors.affiliation: Division of Soil and Fertilizer, National Academy of Agricultural Science, RDA, Wanju, North Jeolla Province 565-851, Republic of Korea.
authors.forename: Yejin
authors.initials: Y
authors.lastname: Lee
authors.affiliation: Division of Soil and Fertilizer, National Academy of Agricultural Science, RDA, Wanju, North Jeolla Province 565-851, Republic of Korea.
authors.forename: Sangkeun
authors.initials: S
authors.lastname: Ha
authors.affiliation: Department of Agronomy, Chungbuk National University, Cheongju, North Chungcheong Province 361-763, Republic of Korea.
authors.forename: Beomheon
authors.initials: B
authors.lastname: Song
authors.affiliation: Department of Plant Life and Environmental Science, Hankyong National University, Anseong, Gyeonggi Province 456-749, Republic of Korea.
authors.forename: Taewan
authors.initials: T
authors.lastname: Kim
authors.affiliation: Department of Agronomy and Horticulture, University of Nebraska, Lincoln, NE, USA.
authors.forename: Brian M
authors.initials: BM
authors.lastname: Waters
authors.affiliation: Plant Genetics Research Unit, Agriculture Research Service, USDA, 205 Curtis Hall, Columbia, MO 65211, USA; Plant Science Division, University of Missouri, 1-41, Agriculture Bldg., Columbia, MO 65211, USA. Electronic address: KrishnanH@missouri.edu.
authors.forename: Hari B
authors.initials: HB
authors.lastname: Krishnan
mesh_terms: D018527:Hydroponics; D018551:Solanum lycopersicum / Q000378:metabolism*; D055442:Metabolome; D009584:Nitrogen / Q000172:deficiency / Q000378:metabolism*; D010758:Phosphorus / Q000172:deficiency / Q000378:metabolism*; D011188:Potassium / Q000378:metabolism*; D036226:Seedlings / Q000378:metabolism
chemical_list: D010758:Phosphorus; D009584:Nitrogen; D011188:Potassium
keywords: Amino acids; Carbohydrates; Metabolomics; Mineral deficiency; Organic acids; Polyamines