title: The Impact of Paeoniflorin on α-Synuclein Degradation Pathway.
abstract: Paeoniflorin (PF) is the major active ingredient in the traditional Chinese medicine Radix. It plays a neuroprotective role by regulating autophagy and the ubiquitin-proteasome degradation pathway. In this study, we found PF significantly reduced cell damage caused by MPP+, returning cells to normal state. Cell viability significantly improved after 24 h exposure to RAPA and PF in the MPP+ group (all P < 0.01). CAT and SOD activities were significantly decreased after PF and RAPA treatment, compared with MPP+ (P < 0.001). In addition, MPP+ activated both LC3-II and E1; RAPA increased LC3-II but inhibited E1. PF significantly upregulated both LC3-II (autophagy) and E1 (ubiquitin-proteasome pathway) expression (P < 0.001), promoted degradation of α-synuclein, and reduced cell damage. We show MPP+ enhanced immunofluorescence signal of intracellular α-synuclein and LC3. Fluorescence intensity of α-synuclein decreased after PF treatment. In conclusion, these data show PF reversed the decline of proteasome activity caused by MPP+ and significantly upregulated both autophagy and ubiquitin-proteasome pathways, promoted the degradation of α-synuclein, and reduced cell damage. These findings suggest PF is a potential therapeutic medicine for neurodegenerative diseases.
authors.affiliation: Lianyungang First People's Hospital Postdoctoral Innovative Practice Base, Nanjing Medical University Postdoctoral Research Station, Lianyungang 222002, China ; Department of Neurology, Affiliated Lianyungang Hospital of Xuzhou Medical College, Lianyungang 222002, China.
authors.forename: Zenglin
authors.identifier: 0000-0003-2884-1429
authors.initials: Z
authors.lastname: Cai
authors.affiliation: Department of Neurology, Affiliated Lianyungang Hospital of Xuzhou Medical College, Lianyungang 222002, China.
authors.forename: Xinzhi
authors.identifier: 0000-0002-7635-1662
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Neurology, Affiliated Lianyungang Hospital of Xuzhou Medical College, Lianyungang 222002, China.
authors.forename: Yongjin
authors.identifier: 0000-0001-5553-2280
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Neurology, Affiliated Lianyungang Hospital of Xuzhou Medical College, Lianyungang 222002, China.
authors.forename: Xiuming
authors.identifier: 0000-0002-1414-6559
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Neurology, Affiliated Lianyungang Hospital of Xuzhou Medical College, Lianyungang 222002, China.
authors.forename: Jing
authors.identifier: 0000-0002-4498-772X
authors.initials: J
authors.lastname: Xu
authors.affiliation: Department of Emergency, Affiliated Lianyungang Hospital of Xuzhou Medical College, Lianyungang 222002, China.
authors.forename: Xiaomin
authors.identifier: 0000-0002-8494-7132
authors.initials: X
authors.lastname: Li