title: Adenoviral Vector-Mediated Delivery of p21WAF1/CIP1Prevents Retinal Neovascularization in an Oxygen-Induced Retinopathy Model.
abstract: To evaluate the inhibitory effect(s) of adenovirus (Ad) vector-mediated delivery of p21WAF1/CIP1(Ad-p21) on retinal neovascularization (RNV) in an animal model of oxygen-induced retinopathy (OIR).
  
  
  
  RNV was determined by examination of retinal fiat mounts and sections postnatal (P) day-17 (P17). Non-perfused retinal areas were analyzed using Image-Pro plus 6.0 software. Reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blot analysis were used to measure mRNA and protein expression of p21 and cyclin-dependent kinase (CDK) 2.
  
  
  
  Compared with phosphate buffer saline (PBS) and Ad-NC group mice, non-perfused retinal areas, neovascularization, and number of endothelial cell nuclei breaking through the internal limiting membrane (ILM) in Ad-p21 group mice were significantly reduced. There were fewer non-perfused retinal areas in Ad-p21 mice than in either PBS or Ad-NC group mice, the differences being significant (F = 101.634; p < 0.05). Levels of p21 mRNA and protein in the Ad-p21 group had increased significantly compared with the other three groups (F = 839.664, 509.817; p < 0.05). Levels of CDK2 mRNA and protein in the Ad-p21 group decreased significantly (F = 301.858, 592.882; p < 0.05).
  
  
  
  Ad-p21 inhibits RNV in OIR. A potential underlying mechanism for this may be that overexpression of p21 arrests the cell cycle at the G1- to S-phase transition via inhibition of CDK2 activity.
authors.affiliation: a Department of Vitreous and Retina , Tianjin Medical University Eye Hospital , Tianjin , China.
authors.forename: Jindong
authors.initials: J
authors.lastname: Han
authors.affiliation: b Department of ICU for Transplantation , Tianjin First Center Hospital , Tianjin , China.
authors.forename: Na
authors.initials: N
authors.lastname: Li
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D000831:Animals, Newborn; D051357:Cyclin-Dependent Kinase 2 / Q000096:biosynthesis / Q000235:genetics*; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000096:biosynthesis / Q000235:genetics*; D004195:Disease Models, Animal; D005786:Gene Expression Regulation; D005822:Genetic Vectors; D051379:Mice; D008810:Mice, Inbred C57BL; D010100:Oxygen / Q000633:toxicity; D012333:RNA, Messenger / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D012160:Retina / Q000187:drug effects / Q000473:pathology; D012164:Retinal Diseases / Q000139:chemically induced; D015861:Retinal Neovascularization / Q000235:genetics / Q000378:metabolism / Q000517:prevention & control*
chemical_list: C493957:Cdkn1a protein, mouse; D050759:Cyclin-Dependent Kinase Inhibitor p21; D012333:RNA, Messenger; C495893:Cdk2 protein, mouse; D051357:Cyclin-Dependent Kinase 2; D010100:Oxygen
keywords: Animal experimentation; gene transfer techniques; retinal neovascularization/prevention and control; transfection