title: Flux Balance Analysis Inspired Bioprocess Upgrading for Lycopene Production by a Metabolically Engineered Strain of Yarrowia lipolytica.
abstract: Genome-scale metabolic models embody a significant advantage of systems biology since their applications as metabolic flux simulation models enable predictions for the production of industrially-interesting metabolites. The biotechnological production of lycopene from Yarrowia lipolytica is an emerging scope that has not been fully scrutinized, especially for what concerns cultivation conditions of newly generated engineered strains. In this study, by combining flux balance analysis (FBA) and Plackett-Burman design, we screened chemicals for lycopene production from a metabolically engineered strain of Y. lipolytica. Lycopene concentrations of 126 and 242 mg/L were achieved correspondingly from the FBA-independent and the FBA-assisted designed media in fed-batch cultivation mode. Transcriptional studies revealed upregulations of heterologous genes in media designed according to FBA, thus implying the efficiency of model predictions. Our study will potentially support upgraded lycopene and other terpenoids production from existing or prospect bioengineered strains of Y. lipolytica and/or closely related yeast species.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China. noelnambou@yahoo.fr.
authors.forename: Komi
authors.initials: K
authors.lastname: Nambou
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China. Y10150130@mail.ecust.edu.cn.
authors.forename: Xingxing
authors.initials: X
authors.lastname: Jian
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China. 010140069@mail.ecust.edu.cn.
authors.forename: Xinkai
authors.initials: X
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China. weiliujing98@163.com.
authors.forename: Liujing
authors.initials: L
authors.lastname: Wei
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China. rene2514@163.com.
authors.forename: Jiajia
authors.initials: J
authors.lastname: Lou
authors.affiliation: INRA, UMR1319 Micalis, Domaine de Vilvert, F-78352 Jouy-en-Josas, France. madzak@platon.grignon.inra.fr.
authors.forename: Catherine
authors.initials: C
authors.lastname: Madzak
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China. qhua@ecust.edu.cn.|Shanghai Collaborative Innovation Center for Biomanufacturing Technology (SCICBT), 130 Meilong Road, Shanghai 200237, China. qhua@ecust.edu.cn.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Hua
keywords: Yarrowia lipolytica; fermentation; flux balance analysis; lycopene; metabolic network model yli v1.7