title: Preliminary evidence of age-dependent clinical signs associated with porcine circovirus 2b in experimentally infected CH3/Rockefeller mice.
abstract: Mice and rats are susceptible to porcine circovirus 2b (PCV2) infection under field and experimental conditions. However, whether PCV2 induces disease in rodents remains a matter of debate. The objectives of the present study were to determine whether PCV2-induced disease in mice is age-dependent and whether intranasally inoculated animals are able to infect animals they come into contact with. Twenty-five CH3/Rockefeller mice were divided into six groups and intranasally inoculated with 25μL of either PCV2b or PBS on days 0, 3 and 6. One group remained untreated. Two age groups were tested: 3-week-old mice and 6-week-old mice. The administration of three PCV2 intranasal inoculations at intervals of three days was able to induce infection and support virus transmission in susceptible mice, regardless of the age at inoculation. The clinical signs associated with PCV2 infection were more severe in younger mice, and PCV2-DNA load was higher in their faeces. In conclusion, PCV2 induced disease in mice.
authors.affiliation: Departamento de Medicina Veterinária Preventiva e Saúde Animal, da Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87, Cidade Universitária, 05508 270, São Paulo, SP Brazil; Complexo Educacional Faculdades Metropolitana Unidas, Veterinária, Rua Ministro Nelson Hungria, 541, Real Parque, Morumbi, São Paulo, SP, Brazil. Electronic address: alessandramarnie@gmail.com.
authors.forename: Alessandra M M G
authors.initials: AM
authors.lastname: de Castro
authors.affiliation: Departamento de Imunologia e Microbiologia, Instituto de Biociência, Universidade Estadual Paulista "Julio de Mesquita Filho", Campus de Botucatu, Botucatu, SP, 18618-970, Brazil; Biotechnology Institute - IBTEC - Sao Paulo State University - UNESP - Campus de Botucatu, SP.
authors.forename: Taís F
authors.initials: TF
authors.lastname: Cruz
authors.affiliation: Departamento de Medicina Veterinária Preventiva e Saúde Animal, da Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87, Cidade Universitária, 05508 270, São Paulo, SP Brazil.
authors.forename: Katarina B
authors.initials: KB
authors.lastname: Yamada
authors.affiliation: Departamento de Clínica e Cirurgia Veterinária, Escola de Veterinária, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627 - Pampulha, 31270-901, Belo Horizonte, MG, Brazil.
authors.forename: Priscilla F
authors.initials: PF
authors.lastname: Gerber
authors.affiliation: Departamento de Clínica e Cirurgia Veterinária, Escola de Veterinária, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627 - Pampulha, 31270-901, Belo Horizonte, MG, Brazil.
authors.forename: Michelle P
authors.initials: MP
authors.lastname: Gabardo
authors.affiliation: Departamento de Imunologia e Microbiologia, Instituto de Biociência, Universidade Estadual Paulista "Julio de Mesquita Filho", Campus de Botucatu, Botucatu, SP, 18618-970, Brazil; Biotechnology Institute - IBTEC - Sao Paulo State University - UNESP - Campus de Botucatu, SP.
authors.forename: João P
authors.initials: JP
authors.lastname: Araújo
authors.affiliation: Departamento de Clínica e Cirurgia Veterinária, Escola de Veterinária, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627 - Pampulha, 31270-901, Belo Horizonte, MG, Brazil.
authors.forename: Roberto M C
authors.initials: RM
authors.lastname: Guedes
authors.affiliation: Departamento de Medicina Veterinária Preventiva e Saúde Animal, da Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87, Cidade Universitária, 05508 270, São Paulo, SP Brazil.
authors.forename: Cinthia K
authors.initials: CK
authors.lastname: Mori
authors.affiliation: Departamento de Medicina Veterinária Preventiva e Saúde Animal, da Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87, Cidade Universitária, 05508 270, São Paulo, SP Brazil.
authors.forename: Camila P
authors.initials: CP
authors.lastname: Oliveira
authors.affiliation: Departamento de Medicina Veterinária Preventiva e Saúde Animal, da Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87, Cidade Universitária, 05508 270, São Paulo, SP Brazil.
authors.forename: Sueli S
authors.initials: SS
authors.lastname: Santos
authors.affiliation: Departamento de Medicina Veterinária Preventiva e Saúde Animal, da Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87, Cidade Universitária, 05508 270, São Paulo, SP Brazil.
authors.forename: Leonardo J
authors.initials: LJ
authors.lastname: Richtzenhain
mesh_terms: D000367:Age Factors; D000818:Animals; D018173:Circoviridae Infections / Q000635:transmission* / Q000821:virology; D017925:Circovirus / Q000235:genetics / Q000502:physiology*; D016679:Genome, Viral; D051379:Mice; D008969:Molecular Sequence Data; D011897:Random Allocation; D012376:Rodent Diseases / Q000635:transmission* / Q000821:virology; D017422:Sequence Analysis, DNA
keywords: PCV2; Rodents; Swine virus; Viral load