title: Stem Cell Replacement Improves Expression of SMP30 in db/db Mice.
abstract: We have previously reported that replacing bone marrow stem cells may improve hyperglycemia and oxidative stress in db/db mice, a type 2 diabetic mouse model. Senescence marker protein 30 (SMP30) is an antioxidant protein that decreases with aging. However, it has not been clear whether SMP30 decreases in the livers of obese mice, and whether stem cell replacement would improve SMP30 expression in the liver. Bone marrow stem cells of db/db mice were replaced with the bone marrow stem cells of C57BL/6 mice. Plasma cytokine and insulin levels were measured, and glycogen content, expression of SMP30, and fibrosis in the liver were assessed. Our results showed that stem cell replacement increased the expression of SMP30 in the liver, resulting from decreased plasma inflammation cytokines and hyperinsulinemia in db/db mice. This is the first report that stem cell replacement increased the expression of SMP30 in the liver, and may help prevent fibrosis in the liver of db/db mice.
authors.affiliation: Department of Stem Cell Disorders, Kansai Medical University, Hirakata City, Osaka 5731010, Japan. liming@hirakata.kmu.ac.jp.
authors.forename: Ming
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Cardiac Surgery, Beijing Anzhen Hospital affiliated to Capital Medical University, Beijing 100029, China. guokequan@hotmail.com.
authors.forename: Kequan
authors.initials: K
authors.lastname: Guo
authors.affiliation: Department of Microbiology, Kansai Medical University, Hirakata City, Osaka 5731010, Japan. taketans@hirakata.kmu.ac.jp.
authors.forename: Shigeru
authors.initials: S
authors.lastname: Taketani
authors.affiliation: Division of Clinical Pathology, Toyooka Hospital, Hyogo 6688501, Japan. adachiya250@gmail.com.
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Adachi
authors.affiliation: Department of Stem Cell Disorders, Kansai Medical University, Hirakata City, Osaka 5731010, Japan. ikehara@hirakata.kmu.ac.jp.
authors.forename: Susumu
authors.initials: S
authors.lastname: Ikehara
mesh_terms: D052242:Adiponectin / Q000097:blood; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D015153:Blotting, Western; D001835:Body Weight; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D016207:Cytokines / Q000097:blood; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000378:metabolism*; D007150:Immunohistochemistry; D007328:Insulin / Q000097:blood; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism*; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008297:Male; D008810:Mice, Inbred C57BL; D010179:Pancreas / Q000473:pathology; D056564:Sirtuin 1 / Q000378:metabolism; D013194:Staining and Labeling; D033581:Stem Cell Transplantation
chemical_list: D052242:Adiponectin; D001786:Blood Glucose; D002135:Calcium-Binding Proteins; D016207:Cytokines; D007328:Insulin; D047908:Intracellular Signaling Peptides and Proteins; C513324:Rgn protein, mouse; D056564:Sirtuin 1
keywords: SMP30; cytokines; fibrosis; stem cell replacement