title: High total antioxidant capacity of the porcine seminal plasma (SP-TAC) relates to sperm survival and fertility.
abstract: The study attempted to clarify the role of total antioxidant capacity of seminal plasma (SP-TAC) on boar sperm survival and fertility after artificial insemination (AI). SP-TAC differed (P < 0.001) among boars (n° = 15) and, to a lesser degree, among ejaculates within male (4 ejaculates/boar). SP-TAC also differed (P < 0.001) among ejaculate fractions (43 ejaculates and 3 fractions per ejaculate), of which the sperm-peak portion of the sperm rich ejaculate fraction (SRF) had the highest SP-TAC. SP-TAC was not correlated with sperm quality (motility and viability) or functionality (intracellular ROS generation and lipid peroxidation) of liquid AI-semen samples stored at 17 °C for 72 h (90 AI-samples), but the decline in sperm quality was larger (P < 0.05) in ejaculates with low, compared with high SP-TAC (hierarchically grouped). The SP-TAC differences among ejaculate portions agree with sperm cryosurvival rates (14 ejaculates from 7 boars), showing sperm from sperm-peak portion better (P < 0.01) post-thaw quality and functionality than those from the entire ejaculate (mainly post-SRF). Boars (n° = 18) with high SP-TAC (hierarchically grouped) had higher (P < 0.05) fertility outcomes (5,546 AI-sows) than those with low SP-TAC. Measurement of SP-TAC ought to be a discriminative tool to prognosis fertility in breeding boars.
authors.affiliation: Department of Medicine and Animal Surgery, Faculty of Veterinary Science, University of Murcia, Spain.
authors.forename: Isabel
authors.initials: I
authors.lastname: Barranco
authors.affiliation: Department of Medicine and Animal Surgery, Faculty of Veterinary Science, University of Murcia, Spain.
authors.forename: Asta
authors.initials: A
authors.lastname: Tvarijonaviciute
authors.affiliation: Department of Medicine and Animal Surgery, Faculty of Veterinary Science, University of Murcia, Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: Perez-Patiño
authors.affiliation: Department of Medicine and Animal Surgery, Faculty of Veterinary Science, University of Murcia, Spain.
authors.forename: Inmaculada
authors.initials: I
authors.lastname: Parrilla
authors.affiliation: Department of Medicine and Animal Surgery, Faculty of Veterinary Science, University of Murcia, Spain.
authors.forename: Jose J
authors.initials: JJ
authors.lastname: Ceron
authors.affiliation: Department of Medicine and Animal Surgery, Faculty of Veterinary Science, University of Murcia, Spain.
authors.forename: Emilio A
authors.initials: EA
authors.lastname: Martinez
authors.affiliation: Department of Clinical &Experimental Medicine (IKE), University of Linköping, Sweden.
authors.forename: Heriberto
authors.initials: H
authors.lastname: Rodriguez-Martinez
authors.affiliation: Department of Medicine and Animal Surgery, Faculty of Veterinary Science, University of Murcia, Spain.
authors.forename: Jordi
authors.initials: J
authors.lastname: Roca
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D002470:Cell Survival; D005298:Fertility; D007315:Insemination, Artificial; D008297:Male; D012661:Semen / Q000378:metabolism*; D055101:Semen Analysis; D013081:Sperm Motility; D013094:Spermatozoa / Q000378:metabolism*; D013552:Swine
chemical_list: D000975:Antioxidants