title: Cytotoxic Nitrobenzoyloxy-substituted Sesquiterpenes from Spongederived Endozoic Fungus Aspergillus insulicola MD10-2.
abstract: The emergence of drug resistance and spread of new infectious diseases necessitated the development of novel antibiotics. Marine sponge-associated fungi represent a reservoir of novel molecules with diverse biological potentials. In this study, we isolated five nitrobenzoyloxy-substituted sesquiterpenes 1-5 from the culture mycelia of an endozoic fungus Aspergillus insulicola MD10-2, obtained from the South China Sea sponge Cinachyrella australiensis. Compound 2 showed cytotoxicity against human lung cancer cell line H-460 with an IC50 value of 6.9 µM. Cytotoxicity of the acetylated derivatives (2a and 2b) of compound 2 decreased markedly, suggesting that the hydroxyl group contributed to the cytotoxic activity. Compound 5 was inactive against H-460, which implied the double bond at C-7 had an effect on cytotoxic activity as well.
authors.forename: Hong-Ying
authors.initials: HY
authors.lastname: Zhao
authors.forename: Ramasamy
authors.initials: R
authors.lastname: Anbuchezhian
authors.forename: Wei
authors.initials: W
authors.lastname: Sun
authors.forename: Chang-Lun
authors.initials: CL
authors.lastname: Shao
authors.forename: Feng-Li
authors.initials: FL
authors.lastname: Zhang
authors.forename: Ying
authors.initials: Y
authors.lastname: Yin
authors.forename: Zhi-Sheng
authors.initials: ZS
authors.lastname: Yu
authors.affiliation: School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai 200240 and School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, People's Republic of China. zyli@sjtu.edu.cn.
authors.forename: Zhi-Yong
authors.initials: ZY
authors.lastname: Li
authors.affiliation: School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai 200240 and School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, People's Republic of China. changyun@ouc.edu.cn.
authors.forename: Chang-Yun
authors.initials: CY
authors.lastname: Wang
mesh_terms: D000818:Animals; D001230:Aspergillus / Q000737:chemistry*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D015394:Molecular Structure; D011161:Porifera / Q000382:microbiology; D012717:Sesquiterpenes / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D012717:Sesquiterpenes