title: Intermolecular interactions at early stage of protein/detergent particle association induced by salt/polyethylene glycol mixtures.
abstract: Mixtures of neutral salts and polyethylene glycol are used for various purposes in biological studies. Although the effects of each component of the mixtures are theoretically well investigated, comprehension of their integrated effects remains insufficient. In this work, their roles and effects as a precipitant were clarified by studying dependence of precipitation curves on salt concentration for integral membrane protein/detergent particles of different physicochemical properties. The dependence of precipitation curves was reasonably related to intermolecular interactions among relevant molecules such as protein, detergent and polyethylene glycol by considering their physicochemical properties. The obtained relationships are useful as basic information to learn the early stage of biological macromolecular associations.
authors.affiliation: National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central-6, 1-1 Higashi, Tsukuba, Ibaraki, 305-8566, Japan. Electronic address: odahara-takayuki@aist.go.jp.
authors.forename: Takayuki
authors.initials: T
authors.lastname: Odahara
authors.affiliation: Fukuoka Agriculture and Forestry Research Center, Chikusino, Fukuoka, 818-8549, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Odahara
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry; D003902:Detergents / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D008565:Membrane Proteins / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D016992:Rhodobacter / Q000378:metabolism; D012241:Rhodopseudomonas / Q000378:metabolism; D012492:Salts / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; D003902:Detergents; D008565:Membrane Proteins; D012492:Salts; D011092:Polyethylene Glycols
keywords: Detergent; Integral membrane protein; Polyethylene glycol; Precipitation curve; Salt