title: Co-culture of oligodendrocytes and neurons can be used to assess drugs for axon regeneration in the central nervous system.
abstract: We present a novel in vitro model in which to investigate the efficacy of experimental drugs for the promotion of axon regeneration in the central nervous system. We co-cultured rat hippocampal neurons and cerebral cortical oligodendrocytes, and tested the co-culture system using a Nogo-66 receptor antagonist peptide (NEP1-40), which promotes axonal growth. Primary cultured oligodendrocytes suppressed axonal growth in the rat hippocampus, but NEP1-40 stimulated axonal growth in the co-culture system. Our results confirm the validity of the neuron-oligodendrocyte co-culture system as an assay for the evaluation of drugs for axon regeneration in the central nervous system.
authors.affiliation: Neurological Hospital of Affiliated Hospital of Logistics University of Chinese People's Armed Police Force, Tianjin, China ; Tianjin University of Traditional Chinese Medicine, Tianjin, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Gang
authors.affiliation: Neurological Hospital of Affiliated Hospital of Logistics University of Chinese People's Armed Police Force, Tianjin, China.
authors.forename: Yu-Chen
authors.initials: YC
authors.lastname: Yao
authors.affiliation: Logistics University of Chinese People's Armed Police Forces, Tianjin, China.
authors.forename: Ying-Fu
authors.initials: YF
authors.lastname: Liu
authors.affiliation: Neurological Hospital of Affiliated Hospital of Logistics University of Chinese People's Armed Police Force, Tianjin, China ; Tianjin University of Traditional Chinese Medicine, Tianjin, China.
authors.forename: Yi-Peng
authors.initials: YP
authors.lastname: Li
authors.affiliation: Neurological Hospital of Affiliated Hospital of Logistics University of Chinese People's Armed Police Force, Tianjin, China ; Liaoning Medical University, Jinzhou, Liaoning Province, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Yang
authors.affiliation: Neurological Hospital of Affiliated Hospital of Logistics University of Chinese People's Armed Police Force, Tianjin, China ; Tianjin University of Traditional Chinese Medicine, Tianjin, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Lu
authors.affiliation: Neurological Hospital of Affiliated Hospital of Logistics University of Chinese People's Armed Police Force, Tianjin, China ; Liaoning Medical University, Jinzhou, Liaoning Province, China.
authors.forename: Yuan-Chi
authors.initials: YC
authors.lastname: Cheng
authors.affiliation: Neurological Hospital of Affiliated Hospital of Logistics University of Chinese People's Armed Police Force, Tianjin, China ; Key Laboratory of Nerve Trauma Repair, Tianjin, China.
authors.forename: Xu-Yi
authors.identifier: 0000-0002-0420-8349
authors.initials: XY
authors.lastname: Chen
authors.affiliation: Neurological Hospital of Affiliated Hospital of Logistics University of Chinese People's Armed Police Force, Tianjin, China ; Key Laboratory of Nerve Trauma Repair, Tianjin, China.
authors.forename: Yue
authors.identifier: 0000-0001-5592-4751
authors.initials: Y
authors.lastname: Tu
keywords: NEP1–40; Nogo; PC12 cells; axon regeneration; experimental models; nerve regeneration; neural regeneration; neurons; oligodendrocytes