title: Formulation and optimization of oxaliplatin immuno-nanoparticles using Box-Behnken design and cytotoxicity assessment for synergistic and receptor-mediated targeting in the treatment of colorectal cancer.
abstract: Conventional chemotherapy majorly lacks clinical application attributed to its inspecificity, adverse effects and inability to penetrate into tumor cells. Hence, the aim of the study was to prepare oxaliplatin solid lipid nanoparticles (OP-SLN) by microemulsion method optimizing it by Box-Behnken design and then covalently conjugated to TRAIL (CD-253) monoclonal antibody (TR-OP-SLN) for targeting colorectal cancer cells. The optimized OP-SLN3 has shown an appreciable particle size (121 ± 1.22 nm), entrapment efficiency (78 ± 0.09%) and drug loading (32 ± 1.01%). Fluorescence study and the Bradford assay further confirmed the binding of the protein. A 1.5-fold increase in cytotoxicity of immuno-nanoparticles (4.9 μM) was observed.
authors.affiliation: a Department of Pharmaceutics , JSS College of Pharmacy (Off-campus), JSS University , Mysore , India.
authors.forename: Shashank
authors.initials: S
authors.lastname: Tummala
authors.affiliation: a Department of Pharmaceutics , JSS College of Pharmacy (Off-campus), JSS University , Mysore , India.
authors.forename: K
authors.initials: K
authors.lastname: Gowthamarajan
authors.affiliation: b Department of Pharmacology , JSS College of Pharmacy (Off-campus), JSS University , Mysore , India.
authors.forename: M N
authors.initials: MN
authors.lastname: Satish Kumar
authors.affiliation: b Department of Pharmacology , JSS College of Pharmacy (Off-campus), JSS University , Mysore , India.
authors.forename: T K
authors.initials: TK
authors.lastname: Praveen
authors.affiliation: c Department of Pharmaceutical analysis , JSS College of Pharmacy (Off-campus), JSS University , Mysore , India.
authors.forename: Karthik
authors.initials: K
authors.lastname: Yamjala
authors.affiliation: d Department of Pharmaceutical Chemistry , JSS College of Pharmacy (Off-campus), JSS University , Mysore , India.
authors.forename: Naga Srinivas
authors.initials: NS
authors.lastname: Tripuraneni
authors.affiliation: a Department of Pharmaceutics , JSS College of Pharmacy (Off-campus), JSS University , Mysore , India.
authors.forename: Ashwati
authors.initials: A
authors.lastname: Prakash
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D000912:Antibodies, Neoplasm / Q000737:chemistry / Q000494:pharmacology; D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000188:drug therapy*; D003603:Cytotoxins / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D016503:Drug Delivery Systems / Q000379:methods*; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry*; D009944:Organoplatinum Compounds / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D000077150:Oxaliplatin; D053221:TNF-Related Apoptosis-Inducing Ligand
chemical_list: D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm; D003603:Cytotoxins; D009944:Organoplatinum Compounds; D053221:TNF-Related Apoptosis-Inducing Ligand; D000077150:Oxaliplatin
keywords: Box–Behnken; immuno-nanoparticles; oxaliplatin; site-specificity