title: Lysozyme as a recognition element for monitoring of bacterial population.
abstract: Bacterial infections remain a significant challenge in biomedicine and environment safety. Increasing worldwide demand for point-of-care techniques and increasing concern on their safe development and use, require a simple and sensitive bioanalysis for pathogen detection. However, this goal is not yet achieved. A design for fluorescein isothiocyanate-labeled lysozyme (FITC-LYZ), which provides quantitative binding information for gram-positive bacteria, Micrococcus luteus, and detects pathogen concentration, is presented. The functional lysozyme is used not only as the pathogenic detection platform, but also as a tracking reagent for microbial population in antibacterial tests. A nonlinear relationship between the system response and the logarithm of the bacterial concentration was observed in the range of 1.2×10(2)-1.2×10(5) cfu mL(-1). The system has a potential for further applications and provides a facile and simple method for detection of pathogenic bacteria. Meanwhile, the fluorescein isothiocyanate -labeled lysozyme is also employed as the tracking agent for antibacterial dynamic assay, which show a similar dynamic curve compared with UV-vis test.
authors.affiliation: Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao 266100, China.
authors.forename: Laibao
authors.initials: L
authors.lastname: Zheng
authors.affiliation: Key Laboratory of Marine Environmental Corrosion and Bio-fouling, Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao 266071, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wan
authors.affiliation: Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao 266100, China. Electronic address: yuyan@ouc.edu.cn.
authors.forename: Liangmin
authors.initials: L
authors.lastname: Yu
authors.affiliation: Key Laboratory of Marine Environmental Corrosion and Bio-fouling, Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao 266071, China. Electronic address: zhangdun@qdio.ac.cn.
authors.forename: Dun
authors.initials: D
authors.lastname: Zhang
mesh_terms: D015374:Biosensing Techniques / Q000191:economics / Q000379:methods*; D004926:Escherichia coli / Q000302:isolation & purification*; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D057230:Limit of Detection; D016982:Micrococcus luteus / Q000302:isolation & purification*; D009113:Muramidase / Q000737:chemistry*
chemical_list: D009113:Muramidase; D016650:Fluorescein-5-isothiocyanate
keywords: Bacterial detection; Bacterial population; Lysozyme; Recognition element