title: Identification of a Lactobacillus plantarum strain that ameliorates chronic inflammation and metabolic disorders in obese and type 2 diabetic mice.
abstract: In this study, we identified a strain of lactic acid bacteria (LAB) that induces high levels of IL-10 production by immune cells, and evaluated the ability of the strain to suppress chronic inflammation and ameliorate metabolic disorders in in vitro and in vivo models. Among a collection of LAB strains, Lactobacillus plantarum strain OLL2712 (OLL2712) induced the highest levels of IL-10 production in mouse-derived dendritic cells and peritoneal macrophages. The anti-inflammatory effects of this strain were evaluated using a co-culture system comprising RAW 264.7 and 3T3-L1 cells. We also administered heat-killed OLL2712 to obese and type 2 diabetic KKAy mice for 3 wk to evaluate the in vivo effects of the strain. The OLL2712 significantly decreased the production of proinflammatory cytokines in vitro. Likewise, the administration of OLL2712 significantly suppressed proinflammatory cytokine levels in both the visceral adipose tissue and the serum of KKAy mice, and reduced serum triglyceride concentrations. The strain also alleviated oxidative stress and adrenaline levels in the serum of KKAy mice. On the other hand, Lactobacillus gasseri strain MEP222804 (a moderate IL-10 inducer) did not ameliorate the systemic inflammation and hyperlipidemia in KKAy mice. Our results suggest that treatment with strong IL-10-inducing LAB has the potential to ameliorate metabolic disorders by suppressing chronic inflammation in the host animal.
authors.affiliation: Lactic Acid Bacteria Research Department, Food Science Research Laboratories, Division of Research and Development, Meiji Co., Ltd., Odawara, Kanagawa, Japan. Electronic address: takayuki.toshimitsu@meiji.com.
authors.forename: T
authors.initials: T
authors.lastname: Toshimitsu
authors.affiliation: Lactic Acid Bacteria Research Department, Food Science Research Laboratories, Division of Research and Development, Meiji Co., Ltd., Odawara, Kanagawa, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Mochizuki
authors.affiliation: Sports Nutrition Research Department, Food Science Research Laboratories, Division of Research and Development, Meiji Co., Ltd., Odawara, Kanagawa, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Ikegami
authors.affiliation: Lactic Acid Bacteria Research Department, Food Science Research Laboratories, Division of Research and Development, Meiji Co., Ltd., Odawara, Kanagawa, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Itou
mesh_terms: D041721:3T3-L1 Cells; D000818:Animals; D018920:Coculture Techniques; D016207:Cytokines / Q000096:biosynthesis; D003713:Dendritic Cells / Q000378:metabolism; D003924:Diabetes Mellitus, Type 2 / Q000150:complications*; D005243:Feces / Q000382:microbiology; D005260:Female; D006801:Humans; D007249:Inflammation / Q000628:therapy*; D016753:Interleukin-10 / Q000096:biosynthesis; D048191:Lactiplantibacillus plantarum / Q000302:isolation & purification* / Q000502:physiology*; D017737:Macrophages, Peritoneal / Q000378:metabolism; D008659:Metabolic Diseases / Q000628:therapy*; D051379:Mice; D008807:Mice, Inbred BALB C; D009765:Obesity / Q000150:complications*; D000067996:RAW 264.7 Cells
chemical_list: D016207:Cytokines; D016753:Interleukin-10
keywords: Lactobacillus plantarum; dendritic cell; hyperlipidemia; inflammation; interleukin-10