title: KillerOrange, a Genetically Encoded Photosensitizer Activated by Blue and Green Light.
abstract: Genetically encoded photosensitizers, proteins that produce reactive oxygen species when illuminated with visible light, are increasingly used as optogenetic tools. Their applications range from ablation of specific cell populations to precise optical inactivation of cellular proteins. Here, we report an orange mutant of red fluorescent protein KillerRed that becomes toxic when illuminated with blue or green light. This new protein, KillerOrange, carries a tryptophan-based chromophore that is novel for photosensitizers. We show that KillerOrange can be used simultaneously and independently from KillerRed in both bacterial and mammalian cells offering chromatic orthogonality for light-activated toxicity.
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia.
authors.forename: Karen S
authors.initials: KS
authors.lastname: Sarkisyan
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia.
authors.forename: Olga A
authors.initials: OA
authors.lastname: Zlobovskaya
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia.|Faculty of Biology, Moscow State University, Moscow, Russia.
authors.forename: Dmitry A
authors.initials: DA
authors.lastname: Gorbachev
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia.
authors.forename: Nina G
authors.initials: NG
authors.lastname: Bozhanova
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia.|Faculty of Medicine, Moscow State University, Moscow, Russia.
authors.forename: George V
authors.initials: GV
authors.lastname: Sharonov
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia.
authors.forename: Dmitriy B
authors.initials: DB
authors.lastname: Staroverov
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia.|Pirogov Russian National Research Medical University, Moscow, Russia.
authors.forename: Evgeny S
authors.initials: ES
authors.lastname: Egorov
authors.affiliation: Laser Biospectroscopy Laboratory, Prokhorov General Physics Institute, Moscow, Russia.
authors.forename: Anastasia V
authors.initials: AV
authors.lastname: Ryabova
authors.affiliation: School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, Georgia, 30332, United States of America.
authors.forename: Kyril M
authors.initials: KM
authors.lastname: Solntsev
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia.|Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russia.
authors.forename: Alexander S
authors.initials: AS
authors.lastname: Mishin
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia.|Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russia.
authors.forename: Konstantin A
authors.initials: KA
authors.lastname: Lukyanov
mesh_terms: D049452:Green Fluorescent Proteins / Q000737:chemistry* / Q000235:genetics; D057809:HEK293 Cells; D006801:Humans; D008164:Luminescent Proteins / Q000737:chemistry* / Q000235:genetics / Q000528:radiation effects / Q000633:toxicity; D017319:Photosensitizing Agents / Q000737:chemistry* / Q000528:radiation effects / Q000633:toxicity; D014466:Ultraviolet Rays; D000097573:Red Fluorescent Protein
chemical_list: D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins; D017319:Photosensitizing Agents; D000097573:Red Fluorescent Protein; C508771:killer red protein, Anthomedusae