title: Flotillin-1 protein is upregulated in human endometrial cancer and localization shifts from epithelial to stromal with increasing tumor grade.
abstract: Endometrial cancer is the most common invasive gynecological malignancy. Flotillin-1 is an integral membrane protein and estrogen responsive gene. Flotillin-1 expression and localization in human endometrial cancers grades 1-3 was investigated using real-time RT-PCR and immunohistochemistry. Flotillin-1 mRNA levels were unchanged in endometrial cancer versus benign endometrium. Flotillin-1 protein was significantly reduced in the epithelial compartment with increasing tumor grade, although levels increased in the tumor stroma across grades. We have identified a novel factor in human endometrial cancer and observed a shift in epithelial to stromal localization with increasing tumor grade in women.
authors.affiliation: a Centre for Reproductive Health , Hudson Institute of Medical Research , Clayton , Victoria , Australia.|b Department of Molecular and Translational Medicine , Monash University , Clayton , Victoria , Australia.|c Department of Anatomy and Developmental Biology , Monash University , Clayton , Victoria , Australia.
authors.forename: Amy Louise
authors.initials: AL
authors.lastname: Winship
authors.affiliation: a Centre for Reproductive Health , Hudson Institute of Medical Research , Clayton , Victoria , Australia.|b Department of Molecular and Translational Medicine , Monash University , Clayton , Victoria , Australia.
authors.forename: Kate
authors.initials: K
authors.lastname: Rainczuk
authors.affiliation: a Centre for Reproductive Health , Hudson Institute of Medical Research , Clayton , Victoria , Australia.|b Department of Molecular and Translational Medicine , Monash University , Clayton , Victoria , Australia.|c Department of Anatomy and Developmental Biology , Monash University , Clayton , Victoria , Australia.
authors.forename: Evdokia
authors.initials: E
authors.lastname: Dimitriadis
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016889:Endometrial Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D004847:Epithelial Cells / Q000378:metabolism* / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D060787:Neoplasm Grading; D021381:Protein Transport; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017154:Stromal Cells / Q000378:metabolism* / Q000473:pathology
chemical_list: D008565:Membrane Proteins; D012333:RNA, Messenger; C106354:flotillins
keywords: Endometrioid; Gynecological cancer; Lipid raft