title: In vitro biofilm production of Candida bloodstream isolates: any association with clinical characteristics?
abstract: Candida spp. are a leading cause of bloodstream infection (BSI) and are associated with high mortality rates. Biofilm production is a virulence factor of Candida spp., and has been linked with poor clinical outcome. The aim of our study was to assess biofilm production of Candida bloodstream isolates at our institute, and to determine whether in vitro biofilm production is associated with any clinical characteristics of infection. During the four-year study period, 93 cases of Candida BSI were analysed. The most frequently isolated species was C. albicans (66.7 %), followed by C. glabrata (9.7 %), C. parapsilosis (9.7 %), C. tropicalis (9.7 %) and C. krusei (4.3 %). Biofilm production was more prevalent among non-albicans Candida spp. (77.4 %) than C. albicans (30.6 %) (P = 0.02). Abdominal surgery was identified as a risk factor of BSI caused by biofilm producing non-albicans Candida isolates. No risk factors predisposing to bloodstream infection caused by a biofilm producing C. albicans isolate were identified. Biofilm production was not verified as a risk factor of mortality.
authors.affiliation: Clinical Microbiology Laboratory, Institute of Laboratory Medicine, Semmelweis University, Budapest, Hungary.
authors.forename: Júlia
authors.initials: J
authors.lastname: Pongrácz
authors.affiliation: Nanomedicine Research and Education Center, Semmelweis University, Budapest, Hungary.
authors.forename: Kálmán
authors.initials: K
authors.lastname: Benedek
authors.affiliation: Clinical Microbiology Laboratory, Institute of Laboratory Medicine, Semmelweis University, Budapest, Hungary.
authors.forename: Emese
authors.initials: E
authors.lastname: Juhász
authors.affiliation: Clinical Microbiology Laboratory, Institute of Laboratory Medicine, Semmelweis University, Budapest, Hungary.
authors.forename: Miklós
authors.initials: M
authors.lastname: Iván
authors.affiliation: Clinical Microbiology Laboratory, Institute of Laboratory Medicine, Semmelweis University, Budapest, Hungary.
authors.forename: Katalin
authors.initials: K
authors.lastname: Kristóf
mesh_terms: D000328:Adult; D000368:Aged; D000935:Antifungal Agents / Q000494:pharmacology; D018441:Biofilms / Q000254:growth & development*; D002175:Candida / Q000145:classification / Q000302:isolation & purification* / Q000472:pathogenicity / Q000502:physiology*; D002176:Candida albicans / Q000302:isolation & purification / Q000502:physiology; D041221:Candida glabrata / Q000302:isolation & purification / Q000502:physiology; D058387:Candidemia / Q000453:epidemiology / Q000382:microbiology* / Q000401:mortality; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D012307:Risk Factors; D037521:Virulence Factors; D055815:Young Adult
chemical_list: D000935:Antifungal Agents; D037521:Virulence Factors