title: Targeted Phosphoproteome Analysis Using Selected/Multiple Reaction Monitoring (SRM/MRM).
abstract: Mass spectrometry-based phosphoproteomics has been rapidly spread based on the advancement of mass spectrometry and development of efficient enrichment techniques for phosphorylated proteins or peptides. Non-targeted approach has been employed in most of the studies for phosphoproteome analysis. However, targeted approach using selected/multiple reaction monitoring (SRM/MRM) is an indispensible technique used for the quantitation of known targets especially when we have many samples to quantitate phosphorylation events on proteins in biological or clinical samples. We herein describe the application of a large-scale phosphoproteome analysis and SRM-based quantitation for the systematic discovery and validation of biomarkers.
authors.affiliation: Laboratory of Proteome Research, National Institute of Biomedical Innovation, Health and Nutrition, 7-6-8 Saitoasagi Ibaraki-shi, Osaka, 567-0085, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Adachi
authors.affiliation: Laboratory for Synthetic Biology, RIKEN Quantitative Biology Center, Kobe, Japan.
authors.forename: Ryohei
authors.initials: R
authors.lastname: Narumi
authors.affiliation: Laboratory of Proteome Research, National Institute of Biomedical Innovation, Health and Nutrition, 7-6-8 Saitoasagi Ibaraki-shi, Osaka, 567-0085, Japan. tomonaga@nibiohn.go.jp.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Tomonaga
mesh_terms: D000818:Animals; D015415:Biomarkers; D002853:Chromatography, Liquid; D006801:Humans; D010455:Peptides; D010750:Phosphoproteins; D020543:Proteome; D040901:Proteomics / Q000379:methods*; D053719:Tandem Mass Spectrometry
chemical_list: D015415:Biomarkers; D010455:Peptides; D010750:Phosphoproteins; D020543:Proteome
keywords: Biomarker; IMAC; Phosphoproteomics; Selected/multiple reaction monitoring; Targeted proteomics