title: Detection and distribution of ostreid herpesvirus 1 in experimentally infected Pacific oyster spat.
abstract: High mortality rates are reported in spat and larvae of Pacific oyster Crassostrea gigas and associated with ostreid herpesvirus 1 (OsHV-1) detection in France. Although the viral infection has been experimentally reproduced in oyster larvae and spat, little knowledge is currently available concerning the viral entry and its distribution in organs and tissues. This study compares OsHV-1 DNA and RNA detection and localization in experimentally infected oysters using two virus doses: a low dose that did not induce any mortality and a high dose inducing high mortality. Real time PCR demonstrated significant differences in terms of viral DNA amounts between the two virus doses. RNA transcripts were detected in oysters receiving the highest dose of viral suspension whereas no transcript was observed in oysters injected with the low dose. This study also allowed observing kinetics of viral DNA and RNA detection in different tissues of oyster spat. Finally, viral detection was significantly different in function of tissues (p<0.005), time (p<0.005) with an interaction between tissues and time (p<0.005) for each probe.
authors.affiliation: Ifremer (Institut Français de Recherche pour l'Exploitation de la Mer), Unité Santé, Génétique et Microbiologie des Mollusques (SG2M), Laboratoire de Génétique et Pathologie des Mollusques Marins (LGPMM), Avenue de Mus de Loup, 17390 La Tremblade, France.
authors.forename: Amélie
authors.initials: A
authors.lastname: Segarra
authors.affiliation: Ifremer (Institut Français de Recherche pour l'Exploitation de la Mer), Unité Santé, Génétique et Microbiologie des Mollusques (SG2M), Laboratoire de Génétique et Pathologie des Mollusques Marins (LGPMM), Avenue de Mus de Loup, 17390 La Tremblade, France.
authors.forename: Laury
authors.initials: L
authors.lastname: Baillon
authors.affiliation: Ifremer (Institut Français de Recherche pour l'Exploitation de la Mer), Unité Santé, Génétique et Microbiologie des Mollusques (SG2M), Laboratoire de Génétique et Pathologie des Mollusques Marins (LGPMM), Avenue de Mus de Loup, 17390 La Tremblade, France.
authors.forename: Nicole
authors.initials: N
authors.lastname: Faury
authors.affiliation: Ifremer (Institut Français de Recherche pour l'Exploitation de la Mer), Unité Santé, Génétique et Microbiologie des Mollusques (SG2M), Laboratoire de Génétique et Pathologie des Mollusques Marins (LGPMM), Avenue de Mus de Loup, 17390 La Tremblade, France.
authors.forename: Delphine
authors.initials: D
authors.lastname: Tourbiez
authors.affiliation: Ifremer (Institut Français de Recherche pour l'Exploitation de la Mer), Unité Santé, Génétique et Microbiologie des Mollusques (SG2M), Laboratoire de Génétique et Pathologie des Mollusques Marins (LGPMM), Avenue de Mus de Loup, 17390 La Tremblade, France. Electronic address: Tristan.Renault@ifremer.fr.
authors.forename: Tristan
authors.initials: T
authors.lastname: Renault
mesh_terms: D000818:Animals; D004279:DNA, Viral / Q000032:analysis / Q000737:chemistry; D006564:Herpesviridae / Q000302:isolation & purification / Q000502:physiology*; D054884:Host-Pathogen Interactions; D017403:In Situ Hybridization; D007700:Kinetics; D007814:Larva / Q000821:virology; D010125:Ostreidae / Q000821:virology*; D012367:RNA, Viral / Q000032:analysis / Q000737:chemistry; D060888:Real-Time Polymerase Chain Reaction; D053586:Virus Internalization
chemical_list: D004279:DNA, Viral; D012367:RNA, Viral
keywords: Crassostrea gigas; In situ hybridization; Ostreid herpesvirus 1; Viral DNA; Viral RNA