title: miRNA profiling in autism spectrum disorder in China.
abstract: Autism spectrum disorder (ASD) is a clinically complex and heterogeneous disorder. It is characterized by impaired social abilities, disordered language, isolated areas of interest, and repetitive behaviors. Evidence suggested that the neuropathology of ASD is widely distributed, involving epigenetic regulation in the brain. MiRNAs are a group of endogenous non-coding RNAs that play a critical role in neurodevelopment, neuroplasticity, and other fundamental neurobiological processes. To study miRNA profiling in Autism spectrum disorder in China, we performed miRNA microarray followed quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Here, we describe detailed methods and analysis on these microarray data which has been deposited in Gene Expression Omnibus (GEO): GSE67979.
authors.affiliation: Department of Neurology & Institute of Translational Medicine at University of South China, The First People's Hospital of Chenzhou, Chenzhou, Hunan 423000, PR China.
authors.forename: Fengzhen
authors.initials: F
authors.lastname: Huang
authors.affiliation: Department of Laboratory Medicine & Institute of Translational Medicine at University of South China, The First People's Hospital of Chenzhou, Chenzhou, Hunan 423000, PR China.
authors.forename: Tieqiao
authors.initials: T
authors.lastname: Zhou
authors.affiliation: Department of Neurology & Institute of Translational Medicine at University of South China, The First People's Hospital of Chenzhou, Chenzhou, Hunan 423000, PR China.
authors.forename: Xiaoxi
authors.initials: X
authors.lastname: Yao
authors.affiliation: Department of Neurology & Institute of Translational Medicine at University of South China, The First People's Hospital of Chenzhou, Chenzhou, Hunan 423000, PR China.
authors.forename: Jiping
authors.initials: J
authors.lastname: Yi
authors.affiliation: Department of Neurology & Institute of Translational Medicine at University of South China, The First People's Hospital of Chenzhou, Chenzhou, Hunan 423000, PR China.
authors.forename: Fei
authors.initials: F
authors.lastname: Zhou
authors.affiliation: Department of Neurology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, PR China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Long
authors.affiliation: Department of Neurology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, PR China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Hou
authors.affiliation: Department of Neurology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, PR China.
authors.forename: Chunrong
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Neurology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, PR China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Department of Neurology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, PR China.
authors.forename: Hong
authors.initials: H
authors.lastname: Jiang
keywords: MiRNA; MiRNA microarray; qRT-PCR