title: Evaluation of antimicrobial action of Carie Care™ and Papacarie Duo™ on Aggregatibacter actinomycetemcomitans a major periodontal pathogen using polymerase chain reaction.
abstract: BACKGROUND
  In the present scenario, we are made available with chemomechanical caries removal system containing a natural proteolytic enzyme for the ease in the excavation of infected dentine. The additive action for these agents is providing antimicrobial and anti-inflammatory properties.
  
  
  OBJECTIVE
  This study was undertaken for assessing the action of Carie Care™ and Papacarie Duo™ on Aggregatibacter actinomycetemcomitans.
  
  
  METHODS
  The samples were collected for cultivation of the periodontal pathogen from the clinical periodontal pockets using sterile paper points. The samples cultured under suitable conditions were analyzed with quantitative polymerase chain reaction targeting 16s r-DNA. The samples were divided into three groups namely, Group A: Control, Group B: With Papacarie Duo, Group C: With Carie Care. The pathogen inoculums plugs were inserted in the petri dishes containing chemically defined medium and the experimental gels at different concentrations and were incubated under optimal conditions. The inhibition of growth of the pathogen was studied visually.
  
  
  RESULTS
  There was visual inhibition of growth for Group B and C and also exhibited a dose-dependent effect also.
  
  
  CONCLUSIONS
  Based on the results of the present study, Carie Care™ gel demonstrated better antimicrobial action against A. actinomycetemcomitans which is a major periodontal disease causing pathogen.
authors.affiliation: Director Research, Vittal Mallya Scienticic Research Foundation, Bengaluru, Karnataka, India.
authors.forename: Anil
authors.initials: A
authors.lastname: Kush
authors.affiliation: Department of Pedodontics and Preventive Dentistry, K.L.E Society's Institute of Dental Sciences, Bengaluru, Karnataka, India.
authors.forename: Rachna
authors.initials: R
authors.lastname: Thakur
authors.affiliation: Department of Pedodontics and Preventive Dentistry, K.L.E Society's Institute of Dental Sciences, Bengaluru, Karnataka, India.
authors.forename: Sandya Devi S
authors.initials: SD
authors.lastname: Patil
authors.affiliation: Department of Pedodontics and Preventive Dentistry, Sir Rajiv Gandhi College of Dental Sciences and Hospital, Bengaluru, Karnataka, India.
authors.forename: Santhosh T
authors.initials: ST
authors.lastname: Paul
authors.affiliation: Department of Pedodontics and Preventive Dentistry, K.L.E Society's Institute of Dental Sciences, Bengaluru, Karnataka, India.
authors.forename: Madhu
authors.initials: M
authors.lastname: Kakanur
keywords: Antimicrobial action; Carie Care; polymerase chain reaction