title: Correlation of Vitreous Vascular Endothelial Growth Factor and Uric Acid Concentration Using Optical Coherence Tomography in Diabetic Macular Edema.
abstract: Purpose. We investigated two factors linked to diabetic macular edema (DME), vitreous and serum levels of vascular endothelial growth factor (VEGF) and uric acid (UA) in patients with DME, and compared the results with changes in optical coherence tomography (OCT) and visual acuity (VA). Methods. A prospective study of 29 eyes, 16 cystoid DME and nonproliferative diabetic retinopathy (DR) and 13 nondiabetic controls. Biochemical analysis of vitreous and serum samples was performed and OCT scans were graded according to central retinal thickness (CRT), cube volume (CV), cube average thickness (CAT), and serous retinal detachment (SRD). Results. In DME group, intravitreal concentrations of VEGF (p < 0.001), UA (p = 0.038), and total protein (p < 0.001) were significantly higher than in control group. In DME subjects, intravitreal UA correlated significantly with intravitreal VEGF (ƍ = 0.559, p = 0.03) but not with total vitreous protein and serum UA. Increased intravitreal VEGF in DME group correlated with increase in CV (ƍ = 0.515/p = 0.041). None of the OCT parameters correlated with the VA. Conclusions. The results suggest that the CV might be assessor of anti-VEGF therapy efficacy. Second, apart from VEGF, the role of UA in the pathogenesis and progression of DR should be considered.
authors.affiliation: Institute of Medical Biochemistry and Laboratory Diagnostics, First Faculty of Medicine, Charles University in Prague and General University Hospital, U Nemocnice 2, 128 08 Prague 2, Czech Republic ; Augenzentrum Augsburg, Prinzregentenstraße 25, 86150 Augsburg, Germany.
authors.forename: Libuse
authors.initials: L
authors.lastname: Krizova
authors.affiliation: Institute of Medical Biochemistry and Laboratory Diagnostics, First Faculty of Medicine, Charles University in Prague and General University Hospital, U Nemocnice 2, 128 08 Prague 2, Czech Republic.
authors.forename: Marta
authors.initials: M
authors.lastname: Kalousova
authors.affiliation: Institute of Medical Biochemistry and Laboratory Diagnostics, First Faculty of Medicine, Charles University in Prague and General University Hospital, U Nemocnice 2, 128 08 Prague 2, Czech Republic.
authors.forename: Ales Antonin
authors.initials: AA
authors.lastname: Kubena
authors.affiliation: Department of Ophthalmology, Faculty of Medicine and Dentistry, Palacky University Olomouc, I. P. Pavlova 6, 775 20 Olomouc, Czech Republic.
authors.forename: Oldrich
authors.identifier: 0000-0002-1403-4936
authors.initials: O
authors.lastname: Chrapek
authors.affiliation: Department of Ophthalmology, Faculty of Medicine and Dentistry, Palacky University Olomouc, I. P. Pavlova 6, 775 20 Olomouc, Czech Republic.
authors.forename: Barbora
authors.initials: B
authors.lastname: Chrapkova
authors.affiliation: Department of Ophthalmology, Faculty of Medicine and Dentistry, Palacky University Olomouc, I. P. Pavlova 6, 775 20 Olomouc, Czech Republic.
authors.forename: Martin
authors.identifier: 0000-0002-5073-9482
authors.initials: M
authors.lastname: Sin
authors.affiliation: Institute of Medical Biochemistry and Laboratory Diagnostics, First Faculty of Medicine, Charles University in Prague and General University Hospital, U Nemocnice 2, 128 08 Prague 2, Czech Republic.
authors.forename: Tomas
authors.initials: T
authors.lastname: Zima