title: Isolation, characterisation and comparative analysis of human umbilical cord vein perivascular cells and cord blood mesenchymal stem cells.
abstract: Perivascular cells are known to be ancestors of mesenchymal stem cells (MSCs) and can be obtained from heart, skin, bone marrow, eye, placenta and umbilical cord (UC). However detailed characterization of perivascular cells around the human UC vein and comparative analysis of them with MSCs haven't been done yet. In this study, our aim is to isolate perivascular cells from human UC vein and characterize them versus UC blood MSCs (UCB-MSCs). For this purpose, perivascular cells around the UC vein were isolated enzymatically and then purified with magnetic activated cell sorting (MACS) method using CD146 Microbead Kit respectively. MSCs were isolated from UCB by Ficoll density gradient solution. Perivascular cells and UCB-MSCs were characterized by osteogenic and adipogenic differentiation procedures, flow cytometric analysis [CD146, CD105, CD31, CD34, CD45 and alpha-smooth muscle actin (α-SMA)], and immunofluorescent staining (MAP1B and Tenascin C). Alizarin red and Oil red O staining results showed that perivascular cells and MSCs had osteogenic and adipogenic differentiation capacity. However, osteogenic differentiation capacity of perivascular cells were found to be less than UCB-MSCs. According to flow cytometric analysis, CD146 expression of perivascular cells were appeared to be 4.8-fold higher than UCB-MSCs. Expression of α-SMA, MAP1B and Tenascin-C from perivascular cells was determined by flow cytometry analysis and immunfluorescent staining. The results appear to support the fact that perivascular cells are the ancestors of MSCs in vascular area. They may be used as alternative cells to MSCs in the field of vascular tissue engineering.
authors.affiliation: Department of Stem Cell Sciences, Graduate School of Health Sciences, Hacettepe University, 06100, Ankara, Turkey.|Center for Stem Cell Research and Development, Hacettepe University, 06100, Ankara, Turkey.
authors.forename: Beyza
authors.initials: B
authors.lastname: Gökçinar-Yagci
authors.affiliation: Department of Obstetrics and Gynecology, Hacettepe University Faculty of Medicine, 06100, Ankara, Turkey.
authors.forename: Özgür
authors.initials: Ö
authors.lastname: Özyüncü
authors.affiliation: Department of Stem Cell Sciences, Graduate School of Health Sciences, Hacettepe University, 06100, Ankara, Turkey. betul.celebi@hacettepe.edu.tr.|Center for Stem Cell Research and Development, Hacettepe University, 06100, Ankara, Turkey. betul.celebi@hacettepe.edu.tr.
authors.forename: Betül
authors.initials: B
authors.lastname: Çelebi-Saltik
mesh_terms: D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation; D002462:Cell Membrane / Q000378:metabolism; D002469:Cell Separation / Q000379:methods*; D048430:Cell Shape; D005312:Fetal Blood / Q000166:cytology*; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D014470:Umbilical Cord / Q000166:cytology*; D014471:Umbilical Veins / Q000166:cytology*
chemical_list: D015415:Biomarkers
keywords: CD146; Mesenchymal stem cells; Perivascular cells; Umbilical cord; Vein