title: Efficacy of Fibroblast Growth Factor on Epithelialization of the Neovagina in Patients with Mayer-Rokitansky-Küster-Hauser Syndrome Who Underwent Vaginoplasty.
abstract: OBJECTIVE
  To evaluate the effect of fibroblast growth factor (FGF) on epithelialization of neovagina in patients with Mayer-Rokitansky-Küster-Hauser syndrome who underwent vaginoplasty.
  
  
  METHODS
  Observational study.
  
  
  METHODS
  University hospital.
  
  
  METHODS
  Seven patients with Mayer-Rokitansky-Küster-Hauser syndrome.
  
  
  METHODS
  Cytological examination was done on vaginal smear samples from the site of completed epithelialization, and tissue was collected from the epithelialized part for histological evaluation. Immunostaining for estrogen receptor α, and keratin 13 and 14, and reverse transcription polymerase chain reaction (RT-PCR) analysis of the FGF receptor (FGFR) 1-4 were performed in samples from case 2 three times (ie, during the surgery, during the period of vaginal creation, and at 3 months and 6 months after the surgery).
  
  
  METHODS
  The primary outcome was the FGF effects on the epithelialization speed and FGFR expression in the neovagina. The second was the role of FGF in the mechanism of vaginal epithelial cell proliferation.
  
  
  RESULTS
  The histological structure of the neovagina was consistent with that of normal vagina. RT-PCR analysis revealed that FGFR was expressed in the control vaginas and neovaginas. Among the FGFR subtypes, FGFR-4 was overexpressed during the process of epithelialization and its level decreased after completion of creation of the new vagina.
  
  
  CONCLUSIONS
  The epithelium of the neovagina was morphologically similar to that of normal vagina. It is suggested that FGF plays the role as a growth factor.
authors.affiliation: Department of Obstetrics and Gynecology, Yokohama City University, Yokohama, Japan. Electronic address: mokomoko0408@yahoo.co.jp.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Nagata
authors.affiliation: Department of Obstetrics and Gynecology, Yokohama City University, Yokohama, Japan.
authors.forename: Aiko
authors.initials: A
authors.lastname: Kawano
authors.affiliation: Department of Obstetrics and Gynecology, Yokohama City University, Yokohama, Japan.
authors.forename: Makiko
authors.initials: M
authors.lastname: Koyama
authors.affiliation: Department of Obstetrics and Gynecology, Yokohama City University, Yokohama, Japan.
authors.forename: Tomomi
authors.initials: T
authors.lastname: Nakamura
authors.affiliation: Department of Obstetrics and Gynecology, Yokohama City University, Yokohama, Japan.
authors.forename: Fumiki
authors.initials: F
authors.lastname: Hirahara
authors.affiliation: Graduate School of Nanobioscience, Yokohama City University, Yokohama, Japan; Department of Biological Science and Technology, Tokyo University of Science, Shinjuku, Tokyo, Japan.
authors.forename: Tadaaki
authors.initials: T
authors.lastname: Nakajima
authors.affiliation: Graduate School of Nanobioscience, Yokohama City University, Yokohama, Japan.
authors.forename: Tomomi
authors.initials: T
authors.lastname: Sato
authors.affiliation: Department of Obstetrics and Gynecology, Yokohama City University, Yokohama, Japan; Department of Gynecology, Yokohama City University Medical Center, Yokohama, Japan.
authors.forename: Hideya
authors.initials: H
authors.lastname: Sakakibara
mesh_terms: D058489:46, XX Disorders of Sex Development / Q000378:metabolism / Q000601:surgery*; D000328:Adult; D000013:Congenital Abnormalities / Q000378:metabolism / Q000601:surgery*; D047628:Estrogen Receptor alpha / Q000378:metabolism; D005260:Female; D005346:Fibroblast Growth Factors / Q000378:metabolism*; D013509:Gynecologic Surgical Procedures; D006801:Humans; D007633:Keratins / Q000378:metabolism; D009095:Mullerian Ducts / Q000002:abnormalities* / Q000378:metabolism / Q000601:surgery; D017468:Receptors, Fibroblast Growth Factor / Q000378:metabolism*; D019651:Plastic Surgery Procedures / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019738:Surgically-Created Structures; D014621:Vagina / Q000187:drug effects / Q000601:surgery*
chemical_list: C506487:ESR1 protein, human; D047628:Estrogen Receptor alpha; D017468:Receptors, Fibroblast Growth Factor; D005346:Fibroblast Growth Factors; D007633:Keratins
keywords: Fibroblast growth factor; Mayer-Rokitansky-Küster-Hauser syndrome; Oxidized regenerated cellulose; Vaginoplasty