title: Label-free SPR detection of gluten peptides in urine for non-invasive celiac disease follow-up.
abstract: Motivated by the necessity of new and efficient methods for dietary gluten control of celiac patients, we have developed a simple and highly sensitive SPR biosensor for the detection of gluten peptides in urine. The sensing methodology enables rapid and label-free quantification of the gluten immunogenic peptides (GIP) by using G12 mAb. The overall performance of the biosensor has been in-depth optimized and evaluated in terms of sensitivity, selectivity and reproducibility, reaching a limit of detection of 0.33 ng mL(-1). Besides, the robustness and stability of the methodology permit the continuous use of the biosensor for more than 100 cycles with excellent repeatability. Special efforts have been focused on preventing and minimizing possible interferences coming from urine matrix enabling a direct analysis in this fluid without requiring extraction or purification procedures. Our SPR biosensor has proven to detect and identify gluten consumption by evaluating urine samples from healthy and celiac individuals with different dietary gluten conditions. This novel biosensor methodology represents a novel approach to quantify the digested gluten peptides in human urine with outstanding sensitivity in a rapid and non-invasive manner. Our technique should be considered as a promising opportunity to develop Point-of-Care (POC) devices for an efficient, simple and accurate gluten free diet (GFD) monitoring as well as therapy follow-up of celiac disease patients.
authors.affiliation: Nanobiosensors and Bioanalytical Applications Group (NanoB2A), Catalan Institute of Nanoscience and Nanotechnology (ICN2), CSIC and The Barcelona Institute of Science and Technology, Campus UAB, Bellaterra, 08193 Barcelona, Spain; CIBER-BBN Networking Center on Bioengineering, Biomaterials and Nanomedicine, Spain.
authors.forename: Maria
authors.initials: M
authors.lastname: Soler
authors.affiliation: CIBER-BBN Networking Center on Bioengineering, Biomaterials and Nanomedicine, Spain; Nanobiosensors and Bioanalytical Applications Group (NanoB2A), Catalan Institute of Nanoscience and Nanotechnology (ICN2), CSIC and The Barcelona Institute of Science and Technology, Campus UAB, Bellaterra, 08193 Barcelona, Spain. Electronic address: mcarmen.estevez@cin2.es.
authors.forename: M-Carmen
authors.initials: MC
authors.lastname: Estevez
authors.affiliation: Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Seville, c/Profesor García González, S/N, 41012 Seville, Spain.
authors.forename: Maria de Lourdes
authors.initials: Mde L
authors.lastname: Moreno
authors.affiliation: Biomedal S.L., c/Américo Vespucio 5, 41092 Seville, Spain.
authors.forename: Angel
authors.initials: A
authors.lastname: Cebolla
authors.affiliation: Nanobiosensors and Bioanalytical Applications Group (NanoB2A), Catalan Institute of Nanoscience and Nanotechnology (ICN2), CSIC and The Barcelona Institute of Science and Technology, Campus UAB, Bellaterra, 08193 Barcelona, Spain; CIBER-BBN Networking Center on Bioengineering, Biomaterials and Nanomedicine, Spain.
authors.forename: Laura M
authors.initials: LM
authors.lastname: Lechuga
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D002446:Celiac Disease / Q000652:urine*; D005500:Follow-Up Studies; D005983:Glutens / Q000276:immunology / Q000652:urine*; D006801:Humans; D010455:Peptides / Q000276:immunology / Q000652:urine*; D020349:Surface Plasmon Resonance / Q000379:methods; D014908:Triticum / Q000737:chemistry
chemical_list: D010455:Peptides; D005983:Glutens
keywords: 33-mer gliadin peptide; Celiac disease; Gluten detection; Gluten immunogenic peptides; POC device; Surface plasmon resonance