title: 3D Tissue Formation of Unilocular Adipocytes in Hydrogel Microfibers.
abstract: Adipose tissue, an active metabolic and endocrine organ mainly composed of unilocular adipocytes, is implicated in various obesity related diseases. Developing morphologically and functionally accurate in vitro models of the adipose tissue is therefore critically important for basic biological studies, drug screening/testing, and clinical implants to advance the understanding and treatment of these diseases. However, current adipose tissue engineering technologies either cannot replicate the unilocular morphologies of mature adipocytes, or lack the ease of monitoring, handling, and scaling up required in the above mentioned applications. This paper presents the differentiation of adipose derived stem cells (ADSCs) to mature adipocytes in highly observable and highly handleable 3D fiber shaped constructs exhibiting morphologies and functions of native adipose tissues. Using the cell fiber technology, ADSCs were encapsulated in hydrogel microfibers, allowed to form into fiber shaped constructs, and differentiated to mature unilocular adipocytes. These adipocyte fibers are observed and maintained for up to 91 d, and secretion of adipose tissue-specific factor, adiponectin, is further confirmed. The handleability of the adipocyte fibers is demonstrated by assembling the adipocyte fibers into doll shaped constructs. Such highly observable, highly handleable, and scalable characteristics of the adipocyte fibers make them suitable for biological studies, high-throughput drug screening/testing, and clinical applications.
authors.affiliation: Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo, 153-8505, Japan.|Takeuchi Biohybrid Innovation Project, Exploratory Research for Advanced Technology (ERATO), Japan Science and Technology Agency (JST), 4-6-1 Komaba, Meguro-ku, Tokyo, 153-8505, Japan.
authors.forename: Amy Y
authors.initials: AY
authors.lastname: Hsiao
authors.affiliation: Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo, 153-8505, Japan.|Takeuchi Biohybrid Innovation Project, Exploratory Research for Advanced Technology (ERATO), Japan Science and Technology Agency (JST), 4-6-1 Komaba, Meguro-ku, Tokyo, 153-8505, Japan.
authors.forename: Teru
authors.initials: T
authors.lastname: Okitsu
authors.affiliation: Faculty of Teacher Education, Shumei University, 1-1 Daigaku-cho, Yachiyo City, Chiba, 276-0003, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Teramae
authors.affiliation: Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo, 153-8505, Japan.|Takeuchi Biohybrid Innovation Project, Exploratory Research for Advanced Technology (ERATO), Japan Science and Technology Agency (JST), 4-6-1 Komaba, Meguro-ku, Tokyo, 153-8505, Japan.
authors.forename: Shoji
authors.initials: S
authors.lastname: Takeuchi
mesh_terms: D017667:Adipocytes / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D050156:Adipogenesis / Q000187:drug effects; D052242:Adiponectin / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002478:Cells, Cultured; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000494:pharmacology*; D021621:Imaging, Three-Dimensional; D013997:Time Factors; D023822:Tissue Engineering / Q000379:methods*
chemical_list: C499918:ADIPOQ protein, human; D052242:Adiponectin; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate
keywords: 3D cell cultures; adipocytes; adipogenesis; adipose derived stem cells; cell fibers