title: Growth Suppression and Induction of Chemosensitivity in Human Gallbladder Epithelial Carcinoma Cells (GBCE) by Adenovirus-Mediated Transfer of the Wild-type p53 Gene.
abstract: OBJECTIVE
  Mutations in the p53 gene are reported in 50~90% of gallbladder and bile duct cancer, and have been implicated in chemoresistance. We undertook this study to determine whether the introduction of the wild type p53 gene into GBCE (human gallbladder cancer cell line with a heterozygous p53 mutation) by an adenoviral vector could increase the sensitivity of the cell to 5-FU, a commonly used drug in the treatment of gallbladder cancer.
  
  
  METHODS
  GBCE cells were transfected with either Ad/p53 or Ad/E1 in the presence of 5-FU. Gene expression was confirmed by western blotting. Nude mice were injected subcutaneously with GBCE cells. When tumors formed, intratumoral injection of Ad/p53 was performed. Reduction of tumor size was compared in two weeks of Ad/p53 gene transfection.
  
  
  RESULTS
  Ad/53 transfection induced a dose-dependent inhibition of tumor growth. Tumor colony formation was more inhibited with p53 gene transfection than with mock transfection in the presence of 5-FU. The reduction in tumor size was more pronounced with p53 transfection than with mock infection.
  
  
  CONCLUSIONS
  These treatment modalities could be utilized in the treatment of p53 mutant human gallbladder cancers.
authors.forename: Sung Bae
authors.initials: SB
authors.lastname: Kim
authors.forename: Myung Hwan
authors.initials: MH
authors.lastname: Kim
authors.forename: Sung Koo
authors.initials: SK
authors.lastname: Lee
authors.forename: Tae Won
authors.initials: TW
authors.lastname: Kim
authors.forename: Cheolwon
authors.initials: C
authors.lastname: Suh
authors.forename: Jeong Sik
authors.initials: JS
authors.lastname: Shin
authors.forename: Jung Sun
authors.initials: JS
authors.lastname: Park
authors.forename: Eun Soon
authors.initials: ES
authors.lastname: Kim
authors.forename: Gyungyub
authors.initials: G
authors.lastname: Gong
authors.forename: Jung Shin
authors.initials: JS
authors.lastname: Lee
authors.forename: Woo Kun
authors.initials: WK
authors.lastname: Kim
authors.forename: Sang Hee
authors.initials: SH
authors.lastname: Kim
keywords: Adenoviral vector; Chemosensitivity; Gallbladder cancer; p53