title: Influence of nitrogen sources on the enzymatic activity and grown by Lentinula edodes in biomass Eucalyptus benthamii.
abstract: Lignocellulose is the most abundant environmental component and a renewable organic resource in soil. There are some filamentous fungi which developed the ability to break down and use cellulose, hemicellulose and lignin as an energy source. The objective of this research was to analyze the effect of three nitrogen resources (ammonium sulfate, saltpetre, soybean) in the holocellulolitic activity of Lentinula edodes EF 50 using as substrate sawdust E. benthamii. An experimental design mixture was applied with repetition in the central point consisting of seven treatments (T) of equal concentrations of nitrogen in ammonium sulfate, potassium nitrate and soybean. The enzymatic activity of avicelase, carboxymetilcellulase, β-glucosidase, xylanases and manganese peroxidase was determined. The humidity, pH, water activity (aw) and qualitative analysis of mycelial growth in 8 times of cultivation were evaluated. The results showed negative effect on enzyme production in treatments with maximum concentration of ammonium sulfate and potassium nitrate. The treatments with cooked soybean flour expressed higher enzymatic activities in times of 3, 6 and 9 days of culture, except in the activity of manganese peroxidase. The highest production was observed in the treatment with ammonium sulfate, and soybean (83.86 UI.L-1) at 20 days of cultivation.
authors.affiliation: Programa de Pós-graduação, Universidade Regional de Blumenau, Blumenau, SC, Brazil.
authors.forename: Z C
authors.initials: ZC
authors.lastname: Pedri
authors.affiliation: Departamento de Engenharia Química, Universidade Regional de Blumenau, Blumenau, SC, Brazil.
authors.forename: L M S
authors.initials: LM
authors.lastname: Lozano
authors.affiliation: Programa de Pós-graduação em Engenharia Ambiental, Universidade Regional de Blumenau, Blumenau, SC, Brazil.
authors.forename: K L
authors.initials: KL
authors.lastname: Hermann
authors.affiliation: Empresa Brasileira de Pesquisa Agropecuária, Colombo, PR, Brazil.
authors.forename: C V
authors.initials: CV
authors.lastname: Helm
authors.affiliation: Universidade Estadual de Maringá, Maringá, PR, Brazil.
authors.forename: R M
authors.initials: RM
authors.lastname: Peralta
authors.affiliation: Universidade Regional de Blumenau, Blumenau, SC, Brazil.
authors.forename: L B B
authors.initials: LB
authors.lastname: Tavares
mesh_terms: D000645:Ammonium Sulfate / Q000378:metabolism; D018533:Biomass; D005052:Eucalyptus / Q000737:chemistry; D008031:Lignin / Q000494:pharmacology*; D009566:Nitrates / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism*; D017680:Potassium Compounds / Q000378:metabolism; D020942:Shiitake Mushrooms / Q000201:enzymology* / Q000254:growth & development*; D013025:Glycine max / Q000737:chemistry; D014934:Wood / Q000032:analysis
chemical_list: D009566:Nitrates; D017680:Potassium Compounds; C036909:lignocellulose; D008031:Lignin; D009584:Nitrogen; C023844:potassium nitrate; D000645:Ammonium Sulfate