title: Profiling of Phenolic Metabolites in Feces from Menopausal Women after Long-Term Isoflavone Supplementation.
abstract: Phenolic compounds were screened by UPLC-ESI-MS/MS in the feces of 15 menopausal women before and after long-term isoflavone treatment. In total, 44 compounds were detected. Large intertreatment, interindividual, and intersample variations were observed in terms of the number of compounds and their concentration. Four compounds, the aglycones daidzein and genistein and the daidzein derivatives dihydrodaidzein and O-desmethylangolensin, were associated with isoflavone metabolism; these were identified only after the isoflavone treatment. In addition, 4-ethylcatechol, 3-hydroxyphenylacetic acid, and 3-phenylpropionic acid differed significantly in pre- and postintervention samples, whereas the concentration of 4-hydroxy-5-phenylvaleric acid showed a trend toward increasing over the treatment. The phenolic profiles of equol-producing and -non-producing groups were similar, with the exceptions of 3-hydroxyphenylacetic acid and 3-phenylpropionic acid, which showed higher concentrations in equol-non-producing women. These findings may help to trace isoflavone-derived metabolites in feces during isoflavone interventions and to design new studies to address their biological effects.
authors.affiliation: Departamento de Microbiología y Bioquı́mica de Productos Lácteos, Instituto de Productos Lácteos de Asturias (IPLA-CSIC) , Paseo Rı́o Linares s/n, 33300 Villaviciosa, Asturias, Spain.|Instituto de Investigación en Ciencias de la Alimentación (CIAL), CSIC-UAM, CEI UAM+CSIC , c/Nicolás Cabrera 9, 28049 Madrid, Spain.
authors.forename: Lucía
authors.initials: L
authors.lastname: Guadamuro
authors.affiliation: Instituto de Investigación en Ciencias de la Alimentación (CIAL), CSIC-UAM, CEI UAM+CSIC , c/Nicolás Cabrera 9, 28049 Madrid, Spain.
authors.forename: Ana M
authors.initials: AM
authors.lastname: Jiménez-Girón
authors.affiliation: Departamento de Microbiología y Bioquı́mica de Productos Lácteos, Instituto de Productos Lácteos de Asturias (IPLA-CSIC) , Paseo Rı́o Linares s/n, 33300 Villaviciosa, Asturias, Spain.
authors.forename: Susana
authors.initials: S
authors.lastname: Delgado
authors.affiliation: Departamento de Microbiología y Bioquı́mica de Productos Lácteos, Instituto de Productos Lácteos de Asturias (IPLA-CSIC) , Paseo Rı́o Linares s/n, 33300 Villaviciosa, Asturias, Spain.
authors.forename: Ana Belén
authors.initials: AB
authors.lastname: Flórez
authors.affiliation: Servicio de Digestivo, Hospital de Cabueñes , Calle Los Prados 395, 33394 Gijón, Spain.
authors.forename: Adolfo
authors.initials: A
authors.lastname: Suárez
authors.affiliation: Instituto de Investigación en Ciencias de la Alimentación (CIAL), CSIC-UAM, CEI UAM+CSIC , c/Nicolás Cabrera 9, 28049 Madrid, Spain.
authors.forename: Pedro J
authors.initials: PJ
authors.lastname: Martín-Álvarez
authors.affiliation: Instituto de Investigación en Ciencias de la Alimentación (CIAL), CSIC-UAM, CEI UAM+CSIC , c/Nicolás Cabrera 9, 28049 Madrid, Spain.
authors.forename: Begoña
authors.initials: B
authors.lastname: Bartolomé
authors.affiliation: Instituto de Investigación en Ciencias de la Alimentación (CIAL), CSIC-UAM, CEI UAM+CSIC , c/Nicolás Cabrera 9, 28049 Madrid, Spain.
authors.forename: M Victoria
authors.initials: MV
authors.lastname: Moreno-Arribas
authors.affiliation: Departamento de Microbiología y Bioquı́mica de Productos Lácteos, Instituto de Productos Lácteos de Asturias (IPLA-CSIC) , Paseo Rı́o Linares s/n, 33300 Villaviciosa, Asturias, Spain.
authors.forename: Baltasar
authors.initials: B
authors.lastname: Mayo
mesh_terms: D019587:Dietary Supplements / Q000032:analysis*; D005243:Feces / Q000737:chemistry*; D005260:Female; D006801:Humans; D007529:Isoflavones / Q000378:metabolism*; D008593:Menopause / Q000378:metabolism*; D008875:Middle Aged; D010636:Phenols / Q000737:chemistry / Q000378:metabolism*; D053719:Tandem Mass Spectrometry; D013997:Time Factors
chemical_list: D007529:Isoflavones; D010636:Phenols
keywords: equol; fecal phenolic metabolites; gut microbiota; isoflavones; menopause