title: Experimental study and thermodynamic modeling for determining the effect of non-polar solvent (hexane)/polar solvent (methanol) ratio and moisture content on the lipid extraction efficiency from Chlorella vulgaris.
abstract: In this research, organic solvent composed of hexane and methanol was used for lipid extraction from dry and wet biomass of Chlorella vulgaris. The results indicated that lipid and fatty acid extraction yield was decreased by increasing the moisture content of biomass. However, the maximum extraction efficiency was attained by applying equivolume mixture of hexane and methanol for both dry and wet biomass. Thermodynamic modeling was employed to estimate the effect of hexane/methanol ratio and moisture content on fatty acid extraction yield. Hansen solubility parameter was used in adjusting the interaction parameters of the model, which led to decrease the number of tuning parameters from 6 to 2. The results indicated that the model can accurately estimate the fatty acid recovery with average absolute deviation percentage (AAD%) of 13.90% and 15.00% for the two cases of using 6 and 2 adjustable parameters, respectively.
authors.affiliation: Department of Chemical and Petroleum Engineering, Sharif University of Technology, Tehran, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Malekzadeh
authors.affiliation: Department of Chemical and Petroleum Engineering, Sharif University of Technology, Tehran, Iran.
authors.forename: Hamed
authors.initials: H
authors.lastname: Abedini Najafabadi
authors.affiliation: Department of Chemical and Petroleum Engineering, Sharif University of Technology, Tehran, Iran.
authors.forename: Maziar
authors.initials: M
authors.lastname: Hakim
authors.affiliation: Department of Chemical and Petroleum Engineering, Sharif University of Technology, Tehran, Iran.
authors.forename: Mehrzad
authors.initials: M
authors.lastname: Feilizadeh
authors.affiliation: Department of Chemical and Petroleum Engineering, Sharif University of Technology, Tehran, Iran; Institute for Biotechnology and Environment (IBE), Sharif University of Technology, Tehran, Iran. Electronic address: vosoughi@sharif.edu.
authors.forename: Manouchehr
authors.initials: M
authors.lastname: Vossoughi
authors.affiliation: Department of Chemical and Petroleum Engineering, Sharif University of Technology, Tehran, Iran.
authors.forename: Davood
authors.initials: D
authors.lastname: Rashtchian
mesh_terms: D018533:Biomass; D048408:Chlorella vulgaris / Q000378:metabolism*; D005227:Fatty Acids / Q000302:isolation & purification; D005612:Freeze Drying; D006586:Hexanes / Q000737:chemistry*; D006813:Humidity; D008055:Lipids / Q000302:isolation & purification*; D000432:Methanol / Q000737:chemistry*; D008962:Models, Theoretical; D012997:Solvents / Q000737:chemistry*; D013816:Thermodynamics
chemical_list: D005227:Fatty Acids; D006586:Hexanes; D008055:Lipids; D012997:Solvents; C026385:n-hexane; D000432:Methanol
keywords: Lipid extraction; Microalgae; Organic solvent; Thermodynamic model; Wet biomass