title: A sandwich-type electrochemical immunosensor for carcinoembryonic antigen based on signal amplification strategy of optimized ferrocene functionalized Fe₃O₄@SiO₂ as labels.
abstract: A sandwich-type electrochemical immunosensor was developed for sensitive detection of carcinoembryonic antigen (CEA) by using ferroferric oxide@silica-amino groups (Fe3O4@SiO2-NH2) as carriers and gold nanoparticles-graphene oxide (GO-AuNPs) as platform. The Fe3O4@SiO2-NH2 surface was used as linked reagents for co-immobilization of ferrocenecarboxylic acid (Fc-COOH) and secondary anti-CEA (Ab2) to prepare the signal probe, and it also could hasten the decomposition of hydrogen peroxide (H2O2) to amplify signals. Differential pulse voltammetry (DPV) was successfully used to quantify CEA. Under the optimized conditions, the designed immunosensor shows an excellent analytical performance wide dynamic response range of CEA concentration from 0.001 ng mL(-1) to 80 ng mL(-1) with a relatively low detection limit of 0.0002 ng mL(-1) (S/N=3), and high specificity and good reproducibility. The proposed immunosensor was successfully used to determine CEA in spiked human serum samples.
authors.affiliation: Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan, Engineering Research Center of Materials-Oriented Chemical Engineering of Xinjiang Production and Construction Corps, Key Laboratory of Materials-Oriented Chemical Engineering of Xinjiang Uygur Autonomous Region, School of Chemistry and Chemical Engineering, Shihezi University, Shihezi 832003, PR China.
authors.forename: Taotao
authors.initials: T
authors.lastname: Feng
authors.affiliation: Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan, Engineering Research Center of Materials-Oriented Chemical Engineering of Xinjiang Production and Construction Corps, Key Laboratory of Materials-Oriented Chemical Engineering of Xinjiang Uygur Autonomous Region, School of Chemistry and Chemical Engineering, Shihezi University, Shihezi 832003, PR China. Electronic address: qxw_tea@shzu.edu.cn.
authors.forename: Xiuwen
authors.initials: X
authors.lastname: Qiao
authors.affiliation: Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan, Engineering Research Center of Materials-Oriented Chemical Engineering of Xinjiang Production and Construction Corps, Key Laboratory of Materials-Oriented Chemical Engineering of Xinjiang Uygur Autonomous Region, School of Chemistry and Chemical Engineering, Shihezi University, Shihezi 832003, PR China.
authors.forename: Haining
authors.initials: H
authors.lastname: Wang
authors.affiliation: Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan, Engineering Research Center of Materials-Oriented Chemical Engineering of Xinjiang Production and Construction Corps, Key Laboratory of Materials-Oriented Chemical Engineering of Xinjiang Uygur Autonomous Region, School of Chemistry and Chemical Engineering, Shihezi University, Shihezi 832003, PR China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Sun
authors.affiliation: Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan, Engineering Research Center of Materials-Oriented Chemical Engineering of Xinjiang Production and Construction Corps, Key Laboratory of Materials-Oriented Chemical Engineering of Xinjiang Uygur Autonomous Region, School of Chemistry and Chemical Engineering, Shihezi University, Shihezi 832003, PR China. Electronic address: hcl_tea@shzu.edu.cn.
authors.forename: Chenglin
authors.initials: C
authors.lastname: Hong
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods; D002272:Carcinoembryonic Antigen / Q000032:analysis / Q000097:blood*; D055664:Electrochemical Techniques / Q000379:methods*; D052203:Ferrosoferric Oxide / Q000737:chemistry*; D005296:Ferrous Compounds / Q000737:chemistry*; D006046:Gold / Q000737:chemistry; D006108:Graphite / Q000737:chemistry; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D007118:Immunoassay / Q000379:methods*; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry; D000075163:Metallocenes; D010087:Oxides / Q000737:chemistry; D015203:Reproducibility of Results; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D055910:Antibodies, Immobilized; D002272:Carcinoembryonic Antigen; D005296:Ferrous Compounds; D000075163:Metallocenes; D010087:Oxides; C032949:ferrocenecarboxylic acid; D006046:Gold; D012822:Silicon Dioxide; D006108:Graphite; D006861:Hydrogen Peroxide; C004998:ferrocene; D052203:Ferrosoferric Oxide
keywords: Amplify signals; Carcinoembryonic antigen; Co-immobilization; Ferrocenecarboxylic acid; Ferroferric oxide@silica