title: Photo-lysine captures proteins that bind lysine post-translational modifications.
abstract: Post-translational modifications (PTMs) have key roles in regulating protein-protein interactions in living cells. However, it remains a challenge to identify these PTM-mediated interactions. Here we develop a new lysine-based photo-reactive amino acid, termed photo-lysine. We demonstrate that photo-lysine, which is readily incorporated into proteins by native mammalian translation machinery, can be used to capture and identify proteins that recognize lysine PTMs, including 'readers' and 'erasers' of histone modifications.
authors.affiliation: Department of Chemistry, University of Hong Kong, Pokfulam Road, Hong Kong, China.
authors.forename: Tangpo
authors.initials: T
authors.lastname: Yang
authors.affiliation: Department of Chemistry, University of Hong Kong, Pokfulam Road, Hong Kong, China.
authors.forename: Xiao-Meng
authors.initials: XM
authors.lastname: Li
authors.affiliation: Department of Chemistry, University of Hong Kong, Pokfulam Road, Hong Kong, China.
authors.forename: Xiucong
authors.initials: X
authors.lastname: Bao
authors.affiliation: Department of Chemistry, University of Hong Kong, Pokfulam Road, Hong Kong, China.
authors.forename: Yi Man Eva
authors.initials: YM
authors.lastname: Fung
authors.affiliation: Department of Chemistry, University of Hong Kong, Pokfulam Road, Hong Kong, China.
authors.forename: Xiang David
authors.initials: XD
authors.lastname: Li
mesh_terms: D000818:Animals; D057930:Click Chemistry; D003978:Diazomethane / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D006367:HeLa Cells; D006657:Histones / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D008027:Light; D008239:Lysine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008958:Models, Molecular; D015394:Molecular Structure; D011485:Protein Binding; D011499:Protein Processing, Post-Translational
chemical_list: D006657:Histones; C000608110:photo-lysine; D003978:Diazomethane; D008239:Lysine