title: Fluorescent Pseudomonads in the Phyllosphere of Wheat: Potential Antagonists Against Fungal Phytopathogens.
abstract: Fluorescent pseudomonads isolated from wheat leaves were characterized regarding their antagonistic potential and taxonomy in relation to protect crop plants from infestation by Fusarium and Alternaria fungi causing diseases in wheat. Using a dual culture assay, inhibition of fungal growth was found for 40 isolates of 175 fluorescent pseudomonads. Twenty-two of the antagonists were able to suppress strains of Fusarium as well as Alternaria. By means of real-time qPCR, the phlD gene encoding the antibiotic 2,4-diacetylphloroglucinol was detected in 20 isolates. On the basis of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry spectral patterns, the isolates with antagonistic activity were assigned to the phylogenetic subgroup Pseudomonas fluorescens and the closely related Pseudomonas gessardii subgroup. The results of the study suggest that pseudomonads in the phyllosphere of crop plants may possibly contribute to natural plant protection.
authors.affiliation: Institute of Landscape Biogeochemistry, Leibniz-Centre for Agricultural Landscape Research (ZALF), Eberswalder Str. 84, 15374, Müncheberg, Germany. tmueller@zalf.de.|Berlin-Brandenburg Institute of Advanced Biodiversity Research (BBIB), Berlin, Germany. tmueller@zalf.de.
authors.forename: Thomas
authors.initials: T
authors.lastname: Müller
authors.affiliation: Institute of Landscape Biogeochemistry, Leibniz-Centre for Agricultural Landscape Research (ZALF), Eberswalder Str. 84, 15374, Müncheberg, Germany.|Berlin-Brandenburg Institute of Advanced Biodiversity Research (BBIB), Berlin, Germany.
authors.forename: Undine
authors.initials: U
authors.lastname: Behrendt
authors.affiliation: Leibniz-Institute of Vegetable and Ornamental Crops, Großbeeren/Erfurt, Theodor-Echtermeyer-Weg 1, 14979, Großbeeren, Germany.
authors.forename: Silke
authors.initials: S
authors.lastname: Ruppel
authors.affiliation: Institute of Landscape Biogeochemistry, Leibniz-Centre for Agricultural Landscape Research (ZALF), Eberswalder Str. 84, 15374, Müncheberg, Germany.|Berlin-Brandenburg Institute of Advanced Biodiversity Research (BBIB), Berlin, Germany.
authors.forename: Grit
authors.initials: G
authors.lastname: von der Waydbrink
authors.affiliation: Institute of Landscape Biogeochemistry, Leibniz-Centre for Agricultural Landscape Research (ZALF), Eberswalder Str. 84, 15374, Müncheberg, Germany.|Berlin-Brandenburg Institute of Advanced Biodiversity Research (BBIB), Berlin, Germany.
authors.forename: Marina E H
authors.initials: ME
authors.lastname: Müller
mesh_terms: D000898:Antibiosis; D001426:Bacterial Proteins / Q000235:genetics; D016000:Cluster Analysis; D005658:Fungi / Q000502:physiology; D018515:Plant Leaves / Q000382:microbiology; D011549:Pseudomonas / Q000145:classification / Q000302:isolation & purification / Q000502:physiology*; D012621:Seasons; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014908:Triticum / Q000382:microbiology*
chemical_list: D001426:Bacterial Proteins; C096183:PhlD protein, Pseudomonas