title: Monoclonal Antibodies Against Porcine sIgA and Their Use in Immunohistochemistry.
abstract: Secretory IgA (sIgA) is known as the predominant immunoglobulin in the mucosal system. It prevents pathogens from invading an animal's body through mucosa, making homeostasis. However, few studies examining the secretion of sIgA in mucosal-associated tissues of porcines based on immunohistochemistry methods have been done. In this study, BALB/c mice were immunized with porcine sIgA and the splenocytes were then fused with myeloma cells. Finally, three hybridoma cell lines secreting monoclonal antibody (MAb) against porcine sIgA were obtained. All three MAbs had no cross-reaction with porcine IgG confirmed by Western blot analysis. Furthermore, lungs, tracheas, and intestines were collected from healthy porcines to prepare tissue slices, followed by incubation with the MAb produced in this study. The results showed that sIgA existing in respiratory and digestive systems could be detected by this newly produced MAb. These generated MAbs against porcine sIgA might have a potential use in mucosal research of porcines.
authors.affiliation: 1 Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, National Research Center for Engineering and Technology of Veterinary Bio-products , Nanjing, China .|2 Key Laboratory of Animal Diseases Diagnostic and Immunology, Ministry of Agriculture, College of Veterinary Medicine, Nanjing Agricultural University , Nanjing, China .
authors.forename: Weixiang
authors.initials: W
authors.lastname: Song
authors.affiliation: 1 Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, National Research Center for Engineering and Technology of Veterinary Bio-products , Nanjing, China .|3 Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses , Yangzhou, China .
authors.forename: Zhixin
authors.initials: Z
authors.lastname: Feng
authors.affiliation: 1 Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, National Research Center for Engineering and Technology of Veterinary Bio-products , Nanjing, China .
authors.forename: Yun
authors.initials: Y
authors.lastname: Bai
authors.affiliation: 1 Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, National Research Center for Engineering and Technology of Veterinary Bio-products , Nanjing, China .
authors.forename: Haiyan
authors.initials: H
authors.lastname: Wang
authors.affiliation: 1 Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, National Research Center for Engineering and Technology of Veterinary Bio-products , Nanjing, China .|4 College of Veterinary Sciences, University of Nyala , Nyala, Sudan .
authors.forename: Hassan Zackaria Ali
authors.initials: HZ
authors.lastname: Ishag
authors.affiliation: 1 Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, National Research Center for Engineering and Technology of Veterinary Bio-products , Nanjing, China .
authors.forename: Ruosong
authors.initials: R
authors.lastname: Yang
authors.affiliation: 1 Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, National Research Center for Engineering and Technology of Veterinary Bio-products , Nanjing, China .
authors.forename: Lizhong
authors.initials: L
authors.lastname: Hua
authors.affiliation: 1 Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, National Research Center for Engineering and Technology of Veterinary Bio-products , Nanjing, China .
authors.forename: Cai
authors.initials: C
authors.lastname: Chen
authors.affiliation: 1 Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, National Research Center for Engineering and Technology of Veterinary Bio-products , Nanjing, China .|2 Key Laboratory of Animal Diseases Diagnostic and Immunology, Ministry of Agriculture, College of Veterinary Medicine, Nanjing Agricultural University , Nanjing, China .
authors.forename: Zhengrong
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: 1 Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, National Research Center for Engineering and Technology of Veterinary Bio-products , Nanjing, China .|2 Key Laboratory of Animal Diseases Diagnostic and Immunology, Ministry of Agriculture, College of Veterinary Medicine, Nanjing Agricultural University , Nanjing, China .
authors.forename: Caisong
authors.initials: C
authors.lastname: Shu
authors.affiliation: 1 Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, National Research Center for Engineering and Technology of Veterinary Bio-products , Nanjing, China .
authors.forename: Maojun
authors.initials: M
authors.lastname: Liu
authors.affiliation: 1 Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, National Research Center for Engineering and Technology of Veterinary Bio-products , Nanjing, China .
authors.forename: Qiyan
authors.initials: Q
authors.lastname: Xiong
authors.affiliation: 1 Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, National Research Center for Engineering and Technology of Veterinary Bio-products , Nanjing, China .|3 Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses , Yangzhou, China .
authors.forename: Guoqing
authors.initials: G
authors.lastname: Shao
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification; D000918:Antibody Specificity; D002459:Cell Fusion; D006825:Hybridomas / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D018928:Immunity, Mucosal; D007071:Immunoglobulin A, Secretory / Q000008:administration & dosage*; D007150:Immunohistochemistry / Q000379:methods*; D007422:Intestines / Q000276:immunology; D008168:Lung / Q000276:immunology; D008214:Lymphocytes / Q000166:cytology / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009101:Multiple Myeloma / Q000276:immunology / Q000473:pathology; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013552:Swine; D014132:Trachea / Q000276:immunology; D014407:Tumor Cells, Cultured
chemical_list: D000911:Antibodies, Monoclonal; D007071:Immunoglobulin A, Secretory