title: Transcriptional profiling of radiation damage and preventive treatments in a 3-dimensional (3D) human cell culture model of oral mucositis.
abstract: Cancer patients who receive radiation are often afflicted by oral mucositis, a debilitating disease, characterized by mouth sores and difficulty in swallowing. Oftentimes, cancer patients afflicted with mucositis must stop life-saving therapies. Thus it is very important to prevent mucositis before it develops. Using a validated organotypic model of human oral mucosa, a 3-dimensional cell culture model of human oral keratinocytes, it has been shown that a mixture (NAC-QYD) of N-acetyl cysteine (NAC) and a traditional Chinese medicine, Qingre Liyan decoction (QYD), prevented radiation damage (Lambros et al., 2014). Here we provide detailed methods and analysis of microarray data for non-irradiated and irradiated human oral mucosal tissue with and without pretreatment with NAC, QYD and NAC-QYD. The microarray data been deposited in Gene Expression Omnibus (GEO): GSE62397. These data can be used to further elucidate the mechanisms of irradiation damage in oral mucosa and its prevention.
authors.affiliation: College of Pharmacy, Western University of Health Sciences, Pomona, CA 91766, USA.
authors.forename: Maria P
authors.initials: MP
authors.lastname: Lambros
authors.affiliation: Gran, LLC, Fullerton, CA 92835, USA.
authors.forename: Michael K
authors.initials: MK
authors.lastname: DeSalvo
authors.affiliation: College of Pharmacy, Western University of Health Sciences, Pomona, CA 91766, USA.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Moreno
authors.affiliation: College of Pharmacy, Western University of Health Sciences, Pomona, CA 91766, USA.
authors.forename: Hari Chandana
authors.initials: HC
authors.lastname: Mulamalla
authors.affiliation: College of Pharmacy, Western University of Health Sciences, Pomona, CA 91766, USA.
authors.forename: Lavanya
authors.initials: L
authors.lastname: Kondapalli