title: Transcriptomes of lineage-specific Drosophila neuroblasts profiled by genetic targeting and robotic sorting.
abstract: A brain consists of numerous distinct neurons arising from a limited number of progenitors, called neuroblasts in Drosophila. Each neuroblast produces a specific neuronal lineage. To unravel the transcriptional networks that underlie the development of distinct neuroblast lineages, we marked and isolated lineage-specific neuroblasts for RNA sequencing. We labeled particular neuroblasts throughout neurogenesis by activating a conditional neuroblast driver in specific lineages using various intersection strategies. The targeted neuroblasts were efficiently recovered using a custom-built device for robotic single-cell picking. Transcriptome analysis of mushroom body, antennal lobe and type II neuroblasts compared with non-selective neuroblasts, neurons and glia revealed a rich repertoire of transcription factors expressed among neuroblasts in diverse patterns. Besides transcription factors that are likely to be pan-neuroblast, many transcription factors exist that are selectively enriched or repressed in certain neuroblasts. The unique combinations of transcription factors present in different neuroblasts may govern the diverse lineage-specific neuron fates.
authors.affiliation: Howard Hughes Medical Institute, Janelia Research Campus, 19700 Helix Drive, Ashburn, VA 20147, USA.
authors.forename: Ching-Po
authors.initials: CP
authors.lastname: Yang
authors.affiliation: Howard Hughes Medical Institute, Janelia Research Campus, 19700 Helix Drive, Ashburn, VA 20147, USA Departments of Bioengineering, Electrical Engineering and Computer Science, and Biophysics Graduate Program, University of California, Berkeley, CA 94720, USA.
authors.forename: Chi-Cheng
authors.initials: CC
authors.lastname: Fu
authors.affiliation: Howard Hughes Medical Institute, Janelia Research Campus, 19700 Helix Drive, Ashburn, VA 20147, USA.
authors.forename: Ken
authors.initials: K
authors.lastname: Sugino
authors.affiliation: Howard Hughes Medical Institute, Janelia Research Campus, 19700 Helix Drive, Ashburn, VA 20147, USA.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Howard Hughes Medical Institute, Janelia Research Campus, 19700 Helix Drive, Ashburn, VA 20147, USA.
authors.forename: Qingzhong
authors.initials: Q
authors.lastname: Ren
authors.affiliation: Howard Hughes Medical Institute, Janelia Research Campus, 19700 Helix Drive, Ashburn, VA 20147, USA.
authors.forename: Ling-Yu
authors.initials: LY
authors.lastname: Liu
authors.affiliation: Howard Hughes Medical Institute, Janelia Research Campus, 19700 Helix Drive, Ashburn, VA 20147, USA.
authors.forename: Xiaohao
authors.initials: X
authors.lastname: Yao
authors.affiliation: Departments of Bioengineering, Electrical Engineering and Computer Science, and Biophysics Graduate Program, University of California, Berkeley, CA 94720, USA.
authors.forename: Luke P
authors.initials: LP
authors.lastname: Lee
authors.affiliation: Howard Hughes Medical Institute, Janelia Research Campus, 19700 Helix Drive, Ashburn, VA 20147, USA leet@janelia.hhmi.org.
authors.forename: Tzumin
authors.initials: T
authors.lastname: Lee
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D019070:Cell Lineage / Q000235:genetics*; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism; D004331:Drosophila melanogaster / Q000166:cytology / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D018390:Gene Targeting; D009474:Neurons / Q000166:cytology*; D012371:Robotics; D017423:Sequence Analysis, RNA; D059010:Single-Cell Analysis; D014157:Transcription Factors / Q000378:metabolism; D059467:Transcriptome / Q000235:genetics*
chemical_list: D029721:Drosophila Proteins; D014157:Transcription Factors
keywords: Antennal lobe; Mushroom body; Neural stem cell; RNA-seq; Transcription factor; Type II neuroblast