title: The role of TRPV1 in the CD4+ T cell-mediated inflammatory response of allergic rhinitis.
abstract: Transient receptor potential vanilloid 1 (TRPV1), which has been identified as a molecular target for the activation of sensory neurons by various painful stimuli, was reported to regulate the signaling and activation of CD4+ T cells. However, the role of TRPV1 in CD4+ T cell in allergic rhinitis remains poorly understood. In this study, TRPV1 expression was localized in CD4+ T cells. Both knockout and chemical inhibition of TRPV1 suppressed Th2/Th17 cytokine production in CD4 T cells and Jurkat T cells, respectively, and can suppress T cell receptor signaling pathways including NF-κB, MAP kinase, and NFAT. In TRPV1 knockout allergic rhinitis (AR) mice, eosinophil infiltration, Th2/Th17 cytokines in the nasal mucosa, and total and ova-specific IgE levels in serum decreased, compared with wild-type AR mice. The TRPV1 antagonists, BCTC or theobromine, showed similar inhibitory immunologic effects on AR mice models. In addition, the number of TRPV1+/CD4+ inflammatory cells increased in the nasal mucosa of patients with AR, compared with that of control subjects. Thus, TRPV1 activation on CD4+ T cells is involved in T cell receptor signaling, and it could be a novel therapeutic target in AR.
authors.affiliation: Department of Otorhinolaryngology, Dankook University College of Medicine, Cheonan, South Korea.|Beckman Laser Institute Korea, Dankook University College of Medicine, Cheonan, South Korea.
authors.forename: Ramachandran
authors.initials: R
authors.lastname: Samivel
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, Boramae Medical Center, Seoul National University College of Medicine, Seoul, South Korea.|Clinical Mucosal Immunology Study Group.
authors.forename: Dae Woo
authors.initials: DW
authors.lastname: Kim
authors.affiliation: Department of Otorhinolaryngology, Dankook University College of Medicine, Cheonan, South Korea.
authors.forename: Hye Ran
authors.initials: HR
authors.lastname: Son
authors.affiliation: Beckman Laser Institute Korea, Dankook University College of Medicine, Cheonan, South Korea.
authors.forename: Yun-Hee
authors.initials: YH
authors.lastname: Rhee
authors.affiliation: Department of Otorhinolaryngology, Dankook University College of Medicine, Cheonan, South Korea.|Beckman Laser Institute Korea, Dankook University College of Medicine, Cheonan, South Korea.
authors.forename: Eun Hee
authors.initials: EH
authors.lastname: Kim
authors.affiliation: Department of Otorhinolaryngology, Dankook University College of Medicine, Cheonan, South Korea.|Beckman Laser Institute Korea, Dankook University College of Medicine, Cheonan, South Korea.
authors.forename: Ji Hye
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Beckman Laser Institute Korea, Dankook University College of Medicine, Cheonan, South Korea.|Department of Premedical Course, Dankook University College of Medicine, Cheonan, South Korea.
authors.forename: Jun-Sang
authors.initials: JS
authors.lastname: Bae
authors.affiliation: Department of Otorhinolaryngology, Dankook University College of Medicine, Cheonan, South Korea.|Beckman Laser Institute Korea, Dankook University College of Medicine, Cheonan, South Korea.
authors.forename: Young-Jun
authors.initials: YJ
authors.lastname: Chung
authors.affiliation: Department of Otorhinolaryngology, Dankook University College of Medicine, Cheonan, South Korea.|Beckman Laser Institute Korea, Dankook University College of Medicine, Cheonan, South Korea.
authors.forename: Phil-Sang
authors.initials: PS
authors.lastname: Chung
authors.affiliation: Department of Medicine, University of California, San Diego, La Jolla, California, USA.
authors.forename: Eyal
authors.initials: E
authors.lastname: Raz
authors.affiliation: Department of Otorhinolaryngology, Dankook University College of Medicine, Cheonan, South Korea.|Beckman Laser Institute Korea, Dankook University College of Medicine, Cheonan, South Korea.|Clinical Mucosal Immunology Study Group.
authors.forename: Ji-Hun
authors.initials: JH
authors.lastname: Mo
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D015153:Blotting, Western; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D016207:Cytokines / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D004804:Eosinophils / Q000276:immunology / Q000378:metabolism; D005260:Female; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology; D007150:Immunohistochemistry; D007249:Inflammation / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D019169:Jurkat Cells; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008875:Middle Aged; D009297:Nasal Mucosa / Q000276:immunology / Q000378:metabolism; D010047:Ovalbumin / Q000276:immunology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D065631:Rhinitis, Allergic / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D050916:TRPV Cation Channels / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D055815:Young Adult
chemical_list: D016207:Cytokines; D050916:TRPV Cation Channels; D007073:Immunoglobulin E; D010047:Ovalbumin
keywords: BCTC; CD4 T lymphocyte; Immune response; Immunity; Immunology and Microbiology Section; OVA; TRPV1; allergic rhinitis