title: Identification of a novel RNA giant nuclear body in cancer cells.
abstract: Constitutive synthesis of oncogenic mRNAs is essential for maintaining the uncontrolled growth of cancer cells. However, little is known about how these mRNAs are exported from the nucleus to the cytoplasm. Here, we report the identification of a RNA giant nuclear body (RNA-GNB) that is abundant in cancer cells but rare in normal cells. The RNA-GNB contains a RNA core surrounded by a protein shell. We identify 782 proteins from cancer-associated RNA-GNBs, 40% of which are involved in the nuclear mRNA trafficking. RNA-GNB is required for cell proliferation, and its abundance is positively associated with tumor burden and outcome of therapies. Our findings suggest that the RNA-GNB is a novel nuclear RNA trafficking organelle that may contribute to the nuclear mRNA exporting and proliferation of cancer cells.
authors.affiliation: Department of Hematology, Key Laboratory of Cancer Prevention and Intervention, China National Ministry of Education, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China.|Cancer Institute of Zhejiang University, Hangzhou 310009, China.|Hangzhou First People's Hospital, Hangzhou 310006, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Cancer Institute of Zhejiang University, Hangzhou 310009, China.|Department of Diabetes Complications and Metabolism, Beckman Research Institute, City of Hope National Medical Center, Duarte, CA 91010, USA.
authors.forename: Jiawei
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Hematology, Key Laboratory of Cancer Prevention and Intervention, China National Ministry of Education, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China.|Cancer Institute of Zhejiang University, Hangzhou 310009, China.|Department of Diabetes Complications and Metabolism, Beckman Research Institute, City of Hope National Medical Center, Duarte, CA 91010, USA.
authors.forename: Ying
authors.initials: Y
authors.lastname: Gu
authors.affiliation: Department of Diabetes Complications and Metabolism, Beckman Research Institute, City of Hope National Medical Center, Duarte, CA 91010, USA.|Zhejiang Academy of Medical Sciences, Hangzhou 310012, China.
authors.forename: Xiaoxian
authors.initials: X
authors.lastname: Gan
authors.affiliation: Department of Hematology, Key Laboratory of Cancer Prevention and Intervention, China National Ministry of Education, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China.|Cancer Institute of Zhejiang University, Hangzhou 310009, China.
authors.forename: Yichao
authors.initials: Y
authors.lastname: Gan
authors.affiliation: Department of Hematology, Key Laboratory of Cancer Prevention and Intervention, China National Ministry of Education, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China.|Cancer Institute of Zhejiang University, Hangzhou 310009, China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Zheng
authors.affiliation: Department of Diabetes Complications and Metabolism, Beckman Research Institute, City of Hope National Medical Center, Duarte, CA 91010, USA.
authors.forename: Byung-Wook
authors.initials: BW
authors.lastname: Kim
authors.affiliation: Department of Hematology, Key Laboratory of Cancer Prevention and Intervention, China National Ministry of Education, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Xu
authors.affiliation: Department of Hematology, Key Laboratory of Cancer Prevention and Intervention, China National Ministry of Education, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China.|Cancer Institute of Zhejiang University, Hangzhou 310009, China.
authors.forename: Xiaoya
authors.initials: X
authors.lastname: Lu
authors.affiliation: Cancer Institute of Zhejiang University, Hangzhou 310009, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Dong
authors.affiliation: Cancer Institute of Zhejiang University, Hangzhou 310009, China.
authors.forename: Shu
authors.initials: S
authors.lastname: Zheng
authors.affiliation: Department of Diabetes Complications and Metabolism, Beckman Research Institute, City of Hope National Medical Center, Duarte, CA 91010, USA.
authors.forename: Wendong
authors.initials: W
authors.lastname: Huang
authors.affiliation: Department of Hematology, Key Laboratory of Cancer Prevention and Intervention, China National Ministry of Education, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China.|Cancer Institute of Zhejiang University, Hangzhou 310009, China.
authors.forename: Rongzhen
authors.initials: R
authors.lastname: Xu
mesh_terms: D015153:Blotting, Western; D016022:Case-Control Studies; D002466:Cell Nucleolus / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism*; D049109:Cell Proliferation; D005455:Fluorescent Antibody Technique; D006801:Humans; D045586:Intranuclear Inclusion Bodies / Q000378:metabolism*; D009369:Neoplasms / Q000378:metabolism* / Q000473:pathology; D009687:Nuclear Proteins / Q000378:metabolism*; D015138:RNA, Nuclear / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D009687:Nuclear Proteins; D015138:RNA, Nuclear
keywords: RNA giant nuclear body; cancer cell; nuclear RNA trafficking