title: Effect of cadmium-polluted diet on growth, salinity stress, hepatotoxicity of juvenile Pacific white shrimp (Litopenaeus vannamei): Protective effect of Zn(II)-curcumin.
abstract: Cadmium (Cd) is one of the major transitional metals that have toxic effects on aquatic organisms. To investigate the effects of dietary cadmium on growth, salinity stress, hepatotoxicity in juvenile Pacific white shrimp (L. vannamei) and potential protective effect of Zn(II)-curcumin, five experimental diets (control, 100mg/kg Zn(II)-curcumin, 30mg/kg Cd, 30mg/kg Cd+100mg/kg Zn(II)-curcumin, 30mg/kg Cd+200mg/kg Zn(II)-curcumin) were formulated. The results showed that Cd at 30mg/kg induced significant increase in weight gain, specific growth rate and visible alterations to the hepatopancreas structures of L. vannamei. Compared with control diet, 100mg/kg Zn(II)-curcumin added diet had no effect on growth performance or feed utilization, while healthier hepatopancreas and less plasma ALT, AST production was found. Moreover, 200mg/kg dietary Zn(II)-curcumin significantly ameliorated the Cd induced hepatotoxicity while 100mg/kg dietary Zn(II)-curcumin slightly ameliorated. Cd accumulation in the whole body was decreasing and Metallothioneins like was increasing in hepatopancreas with increasing dietary Zn(II)-curcumin level. The shrimp fed with dietary Zn(II)-curcumin showed higher survival rate after acute salinity change. Therefore, it can be demonstrated that hepatotoxicity and hormesis could be induced by Cd when Cd levels were 30mg/kg, Zn(II)-curcumin could mitigate the effects of dietary Cd on L. vannamei.
authors.affiliation: Institute of Aquatic Economic Animals, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China; Laboratory of Traditional Chinese Medicine and Marine Drugs, Department of Biochemistry, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China.
authors.forename: Ying-Ying
authors.initials: YY
authors.lastname: Yu
authors.affiliation: Institute of Aquatic Economic Animals, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China.
authors.forename: Shi-Jun
authors.initials: SJ
authors.lastname: Chen
authors.affiliation: Institute of Aquatic Economic Animals, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Chen
authors.affiliation: Institute of Aquatic Economic Animals, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China.
authors.forename: Li-Xia
authors.initials: LX
authors.lastname: Tian
authors.affiliation: Institute of Aquatic Economic Animals, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Niu
authors.affiliation: Institute of Aquatic Economic Animals, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China.
authors.forename: Yong-Jian
authors.initials: YJ
authors.lastname: Liu
authors.affiliation: Laboratory of Traditional Chinese Medicine and Marine Drugs, Department of Biochemistry, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China. Electronic address: donghuixu007@163.com.
authors.forename: Dong-Hui
authors.initials: DH
authors.lastname: Xu
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D002104:Cadmium / Q000008:administration & dosage / Q000633:toxicity*; D003474:Curcumin / Q000737:chemistry / Q000494:pharmacology*; D004032:Diet; D004784:Environmental Monitoring; D043143:Hepatopancreas / Q000187:drug effects; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008668:Metallothionein / Q000378:metabolism; D033561:Penaeidae / Q000187:drug effects* / Q000254:growth & development; D054712:Salinity; D013312:Stress, Physiological / Q000187:drug effects*; D015032:Zinc / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D002104:Cadmium; D008668:Metallothionein; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D003474:Curcumin; D015032:Zinc
keywords: Cadmium; Growth performance; Hepatotoxicity; Litopenaeus vannamei; Zn(II)–curcumin