title: The effect of oleic and palmitic acid on induction of steatosis and cytotoxicity on rat hepatocytes in primary culture.
abstract: In vitro models serve as a tool for studies of steatosis. Palmitic and oleic acids can induce steatosis in cultured hepatocytes. The aim of our study was to verify steatogenic and cytotoxic effects of palmitic acid (PA), oleic acid (OA) and their combinations as well as their impact on functional capacity of rat primary hepatocytes. Hepatocytes were exposed to OA or PA (0.125-2 mmol/l) or their combination at ratios of 3:1, 2:1 or 1:1 at the final concentrations of 0.5-1 mmol/l. Both OA and PA caused a dose-dependent increase in triacylglycerol content in hepatocytes. PA was more steatogenic at 0.25 and 0.5 mmol/l while OA at 0.75 and 1 mmol/l. PA exhibited a dose-dependent cytotoxic effect associated with ROS production, present markers of apoptosis and necrosis and a decrease in albumin production. OA induced a damage of the cytoplasmic membrane from 1 mM concentration. Mixture of OA and PA induced lower cytotoxicity with less weakened functional capacity than did PA alone. Extent of steatosis was comparable to that after exposure to OA alone. In conclusion, OA or combination of OA with PA is more suitable for simulation of simple steatosis than PA alone.
authors.affiliation: Department of Physiology, Charles University in Prague, Faculty of Medicine in Hradec Králové, Hradec Králové, Czech Republic. lotko@lfhk.cuni.cz.
authors.forename: A
authors.initials: A
authors.lastname: Moravcová
authors.forename: Z
authors.initials: Z
authors.lastname: Červinková
authors.forename: O
authors.initials: O
authors.lastname: Kučera
authors.forename: V
authors.initials: V
authors.lastname: Mezera
authors.forename: D
authors.initials: D
authors.lastname: Rychtrmoc
authors.forename: H
authors.initials: H
authors.lastname: Lotková
mesh_terms: D000418:Albumins / Q000378:metabolism; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D022781:Hepatocytes / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D009336:Necrosis; D065626:Non-alcoholic Fatty Liver Disease / Q000139:chemically induced* / Q000378:metabolism / Q000473:pathology; D019301:Oleic Acid / Q000633:toxicity*; D019308:Palmitic Acid / Q000633:toxicity*; D061251:Primary Cell Culture; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D014280:Triglycerides / Q000378:metabolism
chemical_list: D000418:Albumins; D017382:Reactive Oxygen Species; D014280:Triglycerides; D019301:Oleic Acid; D019308:Palmitic Acid