title: Layer-by-Layer assembled growth factor reservoirs for steering the response of 3T3-cells.
abstract: Layer-by-Layer (LbL) assemblies of heparin (Hep) and chitosan (Chi) were prepared for use as reservoirs for acidic and basic fibroblast growth factors (aFGFs and bFGFs, respectively). The effects of the architecture and composition of the reservoirs on the viability and proliferation of NIH-3T3 fibroblast cells were studied under starvation conditions. The reservoir stability was monitored by ellipsometry. The aFGF and bFGF loadings were determined using a dissipation-enhanced quartz crystal microbalance (QCM-D). Stability and release assays were performed in a phosphate buffer at physiological conditions. The results demonstrated that the amount of aFGF and bFGF loaded into and released from LbL reservoirs composed of 3 and 6 layer pairs could be controlled. Cell culture assays in low serum culture medium (LSCM) demonstrated that incorporating very small amounts of aFGF and bFGF into the (Hep/Chi)n multilayers significantly improved the proliferation of the NIH-3T3 fibroblasts. The cells did not proliferate on (Hep/Chi)n assemblies prepared in the absence of FGF under identical conditions. The LbL reservoirs were highly effective for the long-term storage (up to 9 months) of aFGF and bFGF. This work demonstrates the potential of LbL reservoirs for use as biomaterial coatings.
authors.affiliation: C.N.R.S., Institut Charles Sadron, 23 rue du Loess, F-67034 Strasbourg, France; Université de Strasbourg, Faculté de Chimie, 1 rue Blaise Pascal, F-67008 Strasbourg, France. Electronic address: alliny@iq.usp.br.
authors.forename: Alliny F
authors.initials: AF
authors.lastname: Naves
authors.affiliation: C.N.R.S., Institut Charles Sadron, 23 rue du Loess, F-67034 Strasbourg, France; Université de Strasbourg, Faculté de Chimie, 1 rue Blaise Pascal, F-67008 Strasbourg, France.
authors.forename: Marvin
authors.initials: M
authors.lastname: Motay
authors.affiliation: C.N.R.S., Institut Charles Sadron, 23 rue du Loess, F-67034 Strasbourg, France; Université de Strasbourg, Faculté de Chimie, 1 rue Blaise Pascal, F-67008 Strasbourg, France.
authors.forename: Rémi
authors.initials: R
authors.lastname: Mérindol
authors.affiliation: C.N.R.S., Institut Charles Sadron, 23 rue du Loess, F-67034 Strasbourg, France; Centro de Ciências Naturais e Humanas CCNH, Universidade Federal do ABC, Rua Santa Adélia, 166, 09210-170, Santo André, Brazil.
authors.forename: Christiane P
authors.initials: CP
authors.lastname: Davi
authors.affiliation: C.N.R.S., Institut Charles Sadron, 23 rue du Loess, F-67034 Strasbourg, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Felix
authors.affiliation: Departamento de Química Fundamental, Instituto de Química, Universidade de São Paulo, Av. Prof. Lineu Prestes, 748, CP 26077, 05513-970 São Paulo, Brazil.
authors.forename: Luiz H
authors.initials: LH
authors.lastname: Catalani
authors.affiliation: C.N.R.S., Institut Charles Sadron, 23 rue du Loess, F-67034 Strasbourg, France; Université de Strasbourg, Faculté de Chimie, 1 rue Blaise Pascal, F-67008 Strasbourg, France; International Center for Frontier Research in Chemistry, 8 allée Gaspard Monge, F-67083 Strasbourg, France. Electronic address: decher@ics-cnrs.unistra.fr.
authors.forename: Gero
authors.initials: G
authors.lastname: Decher
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry*; D003692:Delayed-Action Preparations / Q000138:chemical synthesis / Q000378:metabolism / Q000494:pharmacology*; D004339:Drug Compounding; D065546:Drug Liberation; D016220:Fibroblast Growth Factor 1 / Q000378:metabolism / Q000494:pharmacology*; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism / Q000494:pharmacology*; D006493:Heparin / Q000737:chemistry*; D007700:Kinetics; D051379:Mice; D041681:NIH 3T3 Cells; D011094:Polyethyleneimine / Q000737:chemistry
chemical_list: D003692:Delayed-Action Preparations; D016222:Fibroblast Growth Factor 2; D016220:Fibroblast Growth Factor 1; D011094:Polyethyleneimine; D006493:Heparin; D048271:Chitosan
keywords: Acidic fibroblast growth factor (aFGF); Basic fibroblast growth factor (bFGF); Chitosan; Controlled release; Heparin; Layer-by-layer assembly; Polyelectrolyte multilayers