title: Proteome profiles of HDL particles of patients with chronic heart failure are associated with immune response and also include bacteria proteins.
abstract: Besides modulation of reverse cholesterol transport, high density lipoprotein (HDL) is able to modulate vascular function by stimulating endothelial nitric oxide synthase. Recently, it could be documented that this function of HDL was significantly impaired in patients with chronic heart failure (CHF). We investigated alterations in the HDL proteome in CHF patients. Therefore, HDL was isolated from 5 controls (HDLhealthy) and 5 CHF patients of NYHA-class IIIb (HDLCHF). Proteome analysis of HDL particles was performed by two-dimensional liquid chromatography-mass spectrometry (SCX/RP LC-MS/MS). In total, we identified 494 distinct proteins, of which 107 proteins were commonly found in both groups (HDLCHF and HDLhealthy) indicating a high inter-subject variability across HDL particles. Several important proteins (e.g. ITGA2, APBA1 or A2M) varied in level. Functional analysis revealed regulated pathways. A minor proportion of bacteria-derived proteins were also identified in the HDL-particles. The extension of the list of HDL-associated proteins allows besides their mere description new insights into alterations in HDL function in diseases. In addition, the detection of bacterial proteins bound to HDL will broaden our view of HDL not only as a cholesterol carrier but also as a carrier of proteins.
authors.affiliation: Department of Cardiac Surgery, University of Leipzig, Heart Center Leipzig, Germany; Division of Diagnostics, Experimental Surgery/CardiOMICs, Fraunhofer-Institute for Cell Therapy and Immunology IZI, Leipzig, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Oberbach
authors.affiliation: Department of Cardiology, University of Leipzig, Heart Center Leipzig, Germany.
authors.forename: Volker
authors.initials: V
authors.lastname: Adams
authors.affiliation: Department of Cardiac Surgery, University of Leipzig, Heart Center Leipzig, Germany; Department of Pediatric Surgery, University of Leipzig, Germany.
authors.forename: Nadine
authors.initials: N
authors.lastname: Schlichting
authors.affiliation: Department of Cardiac Surgery, University of Leipzig, Heart Center Leipzig, Germany.
authors.forename: Marco
authors.initials: M
authors.lastname: Heinrich
authors.affiliation: Department of Cardiac Surgery, University of Leipzig, Heart Center Leipzig, Germany.
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Kullnick
authors.affiliation: Department of Cardiac Surgery, University of Leipzig, Heart Center Leipzig, Germany.
authors.forename: Stefanie
authors.initials: S
authors.lastname: Lehmann
authors.affiliation: Department of Cardiac Surgery, University of Leipzig, Heart Center Leipzig, Germany.
authors.forename: Sven
authors.initials: S
authors.lastname: Lehmann
authors.affiliation: Department of Cardiac Surgery, University of Leipzig, Heart Center Leipzig, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Feder
authors.affiliation: Department of Cardiac Surgery, University of Leipzig, Heart Center Leipzig, Germany.
authors.forename: Joao Carlos
authors.initials: JC
authors.lastname: Correia
authors.affiliation: Department of Cardiac Surgery, University of Leipzig, Heart Center Leipzig, Germany.
authors.forename: Friedrich-Wilhelm
authors.initials: FW
authors.lastname: Mohr
authors.affiliation: Department of Functional Genomics, Interfaculty Institute for Genetics and Functional Genomics, University Medicine Greifswald, Germany; DZHK (German Center for Cardiovascular Research) Partner Site Greifswald, Germany.
authors.forename: Uwe
authors.initials: U
authors.lastname: Völker
authors.affiliation: Department of Functional Genomics, Interfaculty Institute for Genetics and Functional Genomics, University Medicine Greifswald, Germany. Electronic address: nico.jehmlich@ufz.de.
authors.forename: Nico
authors.initials: N
authors.lastname: Jehmlich
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D016022:Case-Control Studies; D002908:Chronic Disease; D005260:Female; D006333:Heart Failure / Q000276:immunology* / Q000378:metabolism* / Q000382:microbiology; D006801:Humans; D008075:Lipoproteins, HDL / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D059748:Proteolysis; D040901:Proteomics
chemical_list: D001426:Bacterial Proteins; D008075:Lipoproteins, HDL
keywords: (Meta)proteomics; HDL functionality; HDL variability; High density lipoprotein (HDL)