title: Highly efficient mesenchymal stem cell proliferation on poly-ε-caprolactone nanofibers with embedded magnetic nanoparticles.
abstract: In this study, we have developed a combined approach to accelerate the proliferation of mesenchymal stem cells (MSCs) in vitro, using a new nanofibrous scaffold made by needleless electrospinning from a mixture of poly-ε-caprolactone and magnetic particles. The biological characteristics of porcine MSCs were investigated while cultured in vitro on composite scaffold enriched with magnetic nanoparticles. Our data indicate that due to the synergic effect of the poly-ε-caprolactone nanofibers and magnetic particles, cellular adhesion and proliferation of MSCs is enhanced and osteogenic differentiation is supported. The cellular and physical attributes make this new scaffold very promising for the acceleration of efficient MSC proliferation and regeneration of hard tissues.
authors.affiliation: Laboratory of Tissue Engineering, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic ; Institute of Biophysics, Second Faculty of Medicine, Charles University in Prague, Prague, Czech Republic.
authors.forename: Jana
authors.initials: J
authors.lastname: Daňková
authors.affiliation: Laboratory of Tissue Engineering, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic ; Faculty of Biomedical Engineering, Czech Technical University in Prague, Kladno, Czech Republic ; University Center for Energy Efficient Buildings, Czech Technical University in Prague, Bustehrad, Czech Republic.
authors.forename: Matej
authors.initials: M
authors.lastname: Buzgo
authors.affiliation: Department of Magnetic Nanosystems, Institute of Physics, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
authors.forename: Jana
authors.initials: J
authors.lastname: Vejpravová
authors.affiliation: Department of Magnetic Nanosystems, Institute of Physics, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
authors.forename: Simona
authors.initials: S
authors.lastname: Kubíčková
authors.affiliation: Laboratory of Tissue Engineering, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic ; Institute of Biophysics, Second Faculty of Medicine, Charles University in Prague, Prague, Czech Republic.
authors.forename: Věra
authors.initials: V
authors.lastname: Sovková
authors.affiliation: University Center for Energy Efficient Buildings, Czech Technical University in Prague, Bustehrad, Czech Republic ; Department of Nonwoven Textiles, Faculty of Textile Engineering, Technical University of Liberec, Liberec, Czech Republic.
authors.forename: Lucie
authors.initials: L
authors.lastname: Vysloužilová
authors.affiliation: Department of Magnetic Nanosystems, Institute of Physics, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
authors.forename: Alice
authors.initials: A
authors.lastname: Mantlíková
authors.affiliation: Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic.
authors.forename: Alois
authors.initials: A
authors.lastname: Nečas
authors.affiliation: Laboratory of Tissue Engineering, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic ; Institute of Biophysics, Second Faculty of Medicine, Charles University in Prague, Prague, Czech Republic ; Faculty of Biomedical Engineering, Czech Technical University in Prague, Kladno, Czech Republic.
authors.forename: Evžen
authors.initials: E
authors.lastname: Amler
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D002208:Caproates / Q000737:chemistry* / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D007783:Lactones / Q000737:chemistry* / Q000494:pharmacology*; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects*; D057139:Nanofibers / Q000737:chemistry*; D011091:Polyesters / Q000494:pharmacology; D013552:Swine; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D002208:Caproates; D007783:Lactones; D058185:Magnetite Nanoparticles; D011091:Polyesters; C121056:caprolactone
keywords: magnetic particles; mesenchymal stem cells; nanofibers; tissue engineering