title: Antisense Deoxyoligonucleotides Inhibit Activities of Matrix Metalloproteinase-2 in Human Fibrosarcoma HT1080 Cells.
abstract: OBJECTIVE
  MMP-2, 72 kDa-type IV collagenase, plays a major role in the migration and growth of tumor cells, a process that requires the disintegration of basement membrane. Activation of MMP-2 is correlated with the invasiveness of various tumors. The aim of this study was to determine the sequence-specific phosphorothioated oligodeoxynucleotides (ODNs) inhibiting the translation of MMP-2 mRNA and the subsequent invasiveness of tumor cells.
  
  
  METHODS
  Eight types of antisense ODNs were designed and each (8micro gram/ml) were transfected into HT1080 cells. The effects of these antisense ODNs on MMP expression were examined by gelatin zymography, Western blot, Northern blot and matrigel assay.
  
  
  RESULTS
  Antisense-5 (+904~923), antisense-6 (+1274~+1293) and antisense-7 (+1646~+1665) reduced the MMP-2 activity of the culture supernatant in HT1080 fibrosarcoma cells. Treatment with antisense-6 showed inhibition of MMP-2 mRNA and protein, and in vitro invasion in a dose-dependent manner.
  
  
  CONCLUSIONS
  Antisense-6 might be one of the therapeutic candidates for tumor invasion and metastasis.
authors.forename: Jung Sun
authors.initials: JS
authors.lastname: Park
authors.forename: Dong On
authors.initials: DO
authors.lastname: Yang
authors.forename: Seon Hee
authors.initials: SH
authors.lastname: Lim
authors.forename: Hyeon Gyeong
authors.initials: HG
authors.lastname: Yoo
authors.forename: Heyon Na
authors.initials: HN
authors.lastname: Cho
authors.forename: Young Do
authors.initials: YD
authors.lastname: Jung
authors.forename: Sae Jong
authors.initials: SJ
authors.lastname: Kim
authors.forename: Sun Sik
authors.initials: SS
authors.lastname: Chung
authors.forename: Boo Ahn
authors.initials: BA
authors.lastname: Shin
keywords: Antisense oligodeoxynucleotides; Matrix metalloproteinases-2; Metastasis; Tumor invasion