title: Assessing sirtuin expression in endometrial carcinoma and non-neoplastic endometrium.
abstract: Sirtuins participate in hormone imbalance, metabolism and aging, which are important processes for endometrial cancer (EC) development. Sirtuins mRNA expression (SIRT1 to 7) was determined in 76 ECs (63 Type I, 12 Type II and one mixed EC), and 30 non-neoplastic endometria (NNE) by quantitative real-time PCR. SIRT1 and SIRT7 protein expression was evaluated by immunohistochemistry using Allred score. Compared to NNE, ECs showed SIRT7 (p < 0.001) mRNA overexpression, whereas SIRT1 (p < 0.001), SIRT2 (p < 0.001), SIRT4 (p < 0.001) and SIRT5 (p < 0.001) were underexpressed. No significant differences were observed for SIRT3 and SIRT6. Type II ECs displayed lower SIRT1 (p = 0.032) and SIRT3 (p = 0.016) transcript levels than Type I ECs. Concerning protein expression, SIRT1 immunostaining median score was higher in ECs compared to NNE epithelium (EC = 5 vs. NNE = 2, p < 0.001), while SIRT7 was lower in ECs (EC = 6 vs. NNE = 7, p < 0.001). No significant associations were found between SIRT1/7 immunoexpression and histological subtype, grade, lymphovascular invasion or stage. Our data shows that sirtuins are deregulated in EC. The diversity of expression patterns observed suggests that sirtuins may have distinctive roles in endometrial cancer similarly to what has been described in other cancer models.
authors.affiliation: Department of Pathology, Portuguese Oncology Institute-Porto (IPO-Porto), Porto, Portugal.|Cancer Epigenetics & Biology Group, Research Center, Portuguese Oncology Institute-Porto, Porto, Portugal.|Department of Pathology and Oncology, Medical Faculty, University of Porto, Porto, Portugal.
authors.forename: Carla
authors.initials: C
authors.lastname: Bartosch
authors.affiliation: Department of Pathology, Portuguese Oncology Institute-Porto (IPO-Porto), Porto, Portugal.|Cancer Epigenetics & Biology Group, Research Center, Portuguese Oncology Institute-Porto, Porto, Portugal.
authors.forename: Sara
authors.initials: S
authors.lastname: Monteiro-Reis
authors.affiliation: Department of Pathology, Portuguese Oncology Institute-Porto (IPO-Porto), Porto, Portugal.|Cancer Epigenetics & Biology Group, Research Center, Portuguese Oncology Institute-Porto, Porto, Portugal.
authors.forename: Diogo
authors.initials: D
authors.lastname: Almeida-Rios
authors.affiliation: Department of Pathology, Portuguese Oncology Institute-Porto (IPO-Porto), Porto, Portugal.
authors.forename: Renata
authors.initials: R
authors.lastname: Vieira
authors.affiliation: Department of Pathology, Centro Hospitalar São João (CHSJ), Porto, Portugal.
authors.forename: Armando
authors.initials: A
authors.lastname: Castro
authors.affiliation: Department of Pathology, Centro Hospitalar São João (CHSJ), Porto, Portugal.
authors.forename: Manuel
authors.initials: M
authors.lastname: Moutinho
authors.affiliation: Department of Pathology, Centro Hospitalar São João (CHSJ), Porto, Portugal.
authors.forename: Marta
authors.initials: M
authors.lastname: Rodrigues
authors.affiliation: Cancer Epigenetics & Biology Group, Research Center, Portuguese Oncology Institute-Porto, Porto, Portugal.
authors.forename: Inês
authors.initials: I
authors.lastname: Graça
authors.affiliation: Department of Pathology and Oncology, Medical Faculty, University of Porto, Porto, Portugal.|Department of Pathology, Centro Hospitalar São João (CHSJ), Porto, Portugal.|IPATIMUP (Institute of Molecular Pathology and Immunology, University of Porto), Porto, Portugal.
authors.forename: José Manuel
authors.initials: JM
authors.lastname: Lopes
authors.affiliation: Cancer Epigenetics & Biology Group, Research Center, Portuguese Oncology Institute-Porto, Porto, Portugal.|Department of Pathology and Molecular Immunology, Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto, Porto, Portugal.
authors.forename: Carmen
authors.initials: C
authors.lastname: Jerónimo
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015153:Blotting, Western; D016889:Endometrial Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D004717:Endometrium / Q000378:metabolism*; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D056564:Sirtuin 1 / Q000235:genetics / Q000378:metabolism; D056565:Sirtuin 2 / Q000235:genetics / Q000378:metabolism; D056566:Sirtuin 3 / Q000235:genetics / Q000378:metabolism; D037761:Sirtuins / Q000235:genetics* / Q000378:metabolism
chemical_list: D024101:Mitochondrial Proteins; C510661:SIRT7 protein, human; C447939:SIRT1 protein, human; C474199:SIRT2 protein, human; C466093:SIRT3 protein, human; C525357:SIRT4 protein, human; C508544:SIRT5 protein, human; C516592:SIRT6 protein, human; D056564:Sirtuin 1; D056565:Sirtuin 2; D056566:Sirtuin 3; D037761:Sirtuins
keywords: Pathology Section; SIRT1; SIRT7; endometrial carcinoma; endometrium; sirtuin