title: Influence of viscoelastic properties of an hyaluronic acid-based hydrogel on viability of mesenchymal stem cells.
abstract: BACKGROUND
  The present research is involved in the framework of the biotherapy using mesenchymal stem cells (MSCs). Here, MSC encapsulation in a hydrogel based on hyaluronic acid (HA) is investigated to optimize the composition of the biomaterial.
  
  
  METHODS
  Several formulations candidates of the hydrogel (9 in total) are postulated as a scaffold for the 3D MSC culture in order to investigate their potential to mimic the in vivo cellular environment. Rheological measurements in oscillation mode of complex modulus and complex viscosity are performed on the different hydrogels. Biological tests are carried out for the measurement of the cell viability of MSC encapsulated in the hydrogels.
  
  
  RESULTS
  Rheological and biological findings are correlated together in order to establish relationships between the viscoelastic properties of the hydrogel and the cellular viability of MSC.
  
  
  CONCLUSIONS
  In the light of the viability results, the composition of the hydrogel was related to the MSC proliferation. Thus, such relations are useful tools for scientists offering them more flexibility in the design of their hydrogels while ensuring an acceptable level of MSC viability.
authors.affiliation: Laboratory PIMM, CNRS UMR 8006, Arts et métiers ParisTech, 151 boulevard de l'hôpital, 75013 Paris, France.
authors.forename: Anissa
authors.initials: A
authors.lastname: Eddhahak
authors.affiliation: Centre de Recherches Chirurgicales, Laboratoire BIOTN, Faculté de médecine, Université Paris-Est Créteil, 8 rue du Général Sarrail, F-94010 Créteil cedex, France.
authors.forename: Mustapha
authors.initials: M
authors.lastname: Zidi
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D002470:Cell Survival / Q000187:drug effects*; D002951:Citrates / Q000737:chemistry / Q000494:pharmacology; D006820:Hyaluronic Acid / Q000737:chemistry* / Q000494:pharmacology; D020100:Hydrogels / Q000737:chemistry; D008297:Male; D059630:Mesenchymal Stem Cells / Q000187:drug effects* / Q000378:metabolism; D008962:Models, Theoretical; D051381:Rats; D011916:Rats, Inbred F344; D012212:Rheology; D055682:Viscoelastic Substances / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D002951:Citrates; D020100:Hydrogels; C070005:Perfadex; D055682:Viscoelastic Substances; D006820:Hyaluronic Acid
keywords: Mesenchymal stem cells (MSCs); cellular viability; hydrogel based on hyaluronic acid; rheology