title: Disposable all-solid-state pH and glucose sensors based on conductive polymer covered hierarchical AuZn oxide.
abstract: Poly(terthiophene benzoic acid) (pTBA) layered-AuZn alloy oxide (AuZnOx) deposited on the screen printed carbon electrode (pTBA/AuZnOx/SPCE) was prepared to create a disposable all-solid-state pH sensor at first. Further, FAD-glucose oxidase (GOx) was immobilized onto the pTBA/AuZnOx/SPCE to fabricate a glucose sensor. The characterizations of the sensor probe reveal that AuZnOx forms a homogeneous hierarchical structure, and that the polymerized pTBA layer on the alloy oxide surface captures GOx covalently. The benzoic acid group of pTBA coated on the probe layer synergetically improved the pH response of the alloy oxide and provide chemical binding sites to enzyme, which resulted in a Nernstian behavior (59.2 ± 0.5 mV/pH) in the pH range of 2-13. The experimental parameters affecting the glucose analysis were studied in terms of pH, temperature, humidity, and interferences. The sensor exhibited a fast response time <1s and a dynamic range between 30 and 500 mg/dL glucose with a detection limit of 17.23 ± 0.32 mg/dL. The reliabilities of the disposable pH and glucose sensors were examined for biological samples.
authors.affiliation: Department of Chemistry and Institute of Biophysio Sensor Technology (IBST), Pusan National University, Busan 609-735, South Korea.
authors.forename: Dong-Min
authors.initials: DM
authors.lastname: Kim
authors.affiliation: Healthcare & Sensing Lab., DMC R&D Center, Samsung Electronics Co. Ltd, Gyeongi-do 443-742, South Korea.
authors.forename: Seong Je
authors.initials: SJ
authors.lastname: Cho
authors.affiliation: Healthcare & Sensing Lab., DMC R&D Center, Samsung Electronics Co. Ltd, Gyeongi-do 443-742, South Korea.
authors.forename: Chul-Ho
authors.initials: CH
authors.lastname: Cho
authors.affiliation: Healthcare & Sensing Lab., DMC R&D Center, Samsung Electronics Co. Ltd, Gyeongi-do 443-742, South Korea.
authors.forename: Kwang Bok
authors.initials: KB
authors.lastname: Kim
authors.affiliation: Department of Chemistry and Institute of Biophysio Sensor Technology (IBST), Pusan National University, Busan 609-735, South Korea.
authors.forename: Min-Yeong
authors.initials: MY
authors.lastname: Kim
authors.affiliation: Department of Chemistry and Institute of Biophysio Sensor Technology (IBST), Pusan National University, Busan 609-735, South Korea. Electronic address: ybshim@pusan.ac.kr.
authors.forename: Yoon-Bo
authors.initials: YB
authors.lastname: Shim
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D005947:Glucose / Q000737:chemistry / Q000302:isolation & purification*; D005949:Glucose Oxidase / Q000737:chemistry*; D006046:Gold / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D010087:Oxides / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D015032:Zinc / Q000737:chemistry
chemical_list: D010087:Oxides; D011108:Polymers; D006046:Gold; D005949:Glucose Oxidase; D005947:Glucose; D015032:Zinc
keywords: AuZn oxide; Conductive polymer; Disposable potentiometric glucose sensor; Enzyme sensor; pH sensor