title: Water-compatible molecularly imprinted polymers for selective solid phase extraction of dencichine from the aqueous extract of Panax notoginseng.
abstract: Specific molecularly imprinted polymers for dencichine were developed for the first time in this study by the bulk polymerization using phenylpyruvic acid and dl-tyrosine as multi-templates. The photographs confirmed that molecularly imprinted polymers prepared using N,N'-methylene diacrylamide as cross-linker and glycol dimethyl ether as porogen displayed excellent hydrophilicity. Selectivity, adsorption isotherm and adsorption kinetics were investigated. The sample loading-washing-eluting solvent was optimized to evaluate the property of molecularly imprinted solid phase extract. Compared with LC/WCX-SPE, water-compatible molecularly imprinted solid phase extraction displayed more excellent specific adsorption performance. The extracted dencichine from Panax notoginseng with the purity of 98.5% and the average recovery of 85.6% (n=3) was obtained.
authors.affiliation: Key Laboratory of TCM Quality Control Technology, Shandong Analysis and Test Center, Shandong Academy of Sciences, 19 Keyuan Street, Jinan 250014, China.
authors.forename: Wenhua
authors.initials: W
authors.lastname: Ji
authors.affiliation: College of Food Science and Engineering, Shandong Agricultural University, Taian 270018, China.
authors.forename: Hongkai
authors.initials: H
authors.lastname: Xie
authors.affiliation: Key Laboratory of TCM Quality Control Technology, Shandong Analysis and Test Center, Shandong Academy of Sciences, 19 Keyuan Street, Jinan 250014, China; National Resource Center for Chinese Materia Medica, State Key Laboratory Breeding Base of Dao-di Herbs, China Academy of Chinese Medical Sciences, Beijing 100700, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Key Laboratory of TCM Quality Control Technology, Shandong Analysis and Test Center, Shandong Academy of Sciences, 19 Keyuan Street, Jinan 250014, China; College of Food Science and Engineering, Shandong Agricultural University, Taian 270018, China. Electronic address: wangx@sdas.org.
authors.forename: Xiao
authors.initials: X
authors.lastname: Wang
authors.affiliation: College of Food Science and Engineering, Shandong Agricultural University, Taian 270018, China.
authors.forename: Xiuli
authors.initials: X
authors.lastname: Ma
authors.affiliation: National Resource Center for Chinese Materia Medica, State Key Laboratory Breeding Base of Dao-di Herbs, China Academy of Chinese Medical Sciences, Beijing 100700, China. Electronic address: luqihuang@126.com.
authors.forename: Luqi
authors.initials: L
authors.lastname: Huang
mesh_terms: D000599:Amino Acids, Diamino / Q000302:isolation & purification*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D054802:Molecular Imprinting; D011108:Polymers / Q000737:chemistry*; D015203:Reproducibility of Results; D052616:Solid Phase Extraction / Q000379:methods*; D014867:Water / Q000737:chemistry
chemical_list: D000599:Amino Acids, Diamino; D004365:Drugs, Chinese Herbal; C519374:Panax notoginseng extract; D011108:Polymers; D014867:Water; C100126:oxalyldiaminopropionic acid
keywords: Dencichine; HPLC; Molecularly imprinted polymers; Natural products; Panax notoginseng