title: Preparation, analysis and antioxidant evaluation of the controlled product of polysaccharide from Mactra veneriformis by mild acid hydrolysis.
abstract: The polysaccharides from Mactra veneriformis (MVPS) were degraded by controlled mild acid hydrolysis to produce active oligosaccharides. MVPS can easily be hydrolyzed by H2SO4 or HCl. The hydrolyzing process was investigated in acid addition and reaction time by evaluating the producing content of reducing sugar. Hydrolysis with 1M HCl to MVPS can generate a time-depended behavior that is mild and controllable. HPLC analysis monitored the change of oligosaccharides composition in hydrolyzing. Total nine oligosaccharides are recognizable in the HPLC profile, and their content showed a regular transformation in hydrolysis. Those nine ingredients were identified as glucooligosaccharides with DP from 1 to 7 by MS analysis. Antioxidant activities of the typical hydrolyzates as well as MVPs were further tested in assays of DPPH and hydroxyl radicals scavenging, and reducing power. It was found that HCl hydrolyzate exhibited stronger antioxidant effects than MVPS and H2SO4 hydrolyzate due to its higher content of oligosaccharides.
authors.affiliation: School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, PR China; Jiangsu Key Laboratory of Research and Development in Marine Bio-resource Pharmaceutics, Nanjing University of Chinese Medicine, Nanjing 210023, PR China. Electronic address: 993wlc@njucm.edu.cn.
authors.forename: Ling Chong
authors.initials: LC
authors.lastname: Wang
authors.affiliation: School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, PR China; Jiangsu Key Laboratory of Research and Development in Marine Bio-resource Pharmaceutics, Nanjing University of Chinese Medicine, Nanjing 210023, PR China. Electronic address: whao5795@vip.sina.com.
authors.forename: Hao
authors.initials: H
authors.lastname: Wu
authors.affiliation: Center for Drug Safety Evaluation and Research, Nanjing University of Chinese Medicine, 210023, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Ji
authors.affiliation: School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, PR China.
authors.forename: Feng
authors.initials: F
authors.lastname: Xue
authors.affiliation: School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, PR China; Jiangsu Key Laboratory of Research and Development in Marine Bio-resource Pharmaceutics, Nanjing University of Chinese Medicine, Nanjing 210023, PR China.
authors.forename: Rui
authors.initials: R
authors.lastname: Liu
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology; D049872:Bivalvia / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D006868:Hydrolysis; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology; D012995:Solubility; D014867:Water / Q000737:chemistry
chemical_list: D000975:Antioxidants; D011134:Polysaccharides; D014867:Water
keywords: Alpha-d-glucose (PubChem CID: 79025); Antioxidant activity; Hydrolyzation; Kojibiose (PubChem CID: 164939); M. veneriformis; Maltose (PubChem CID: 109991481); Maltotriose (PubChem CID: 109991481); Oligosaccharide; Polysaccharide