title: Antiviral activity and specific modes of action of bacterial prodigiosin against Bombyx mori nucleopolyhedrovirus in vitro.
abstract: Prodigiosin, the tripyrrole red pigment, is a bacterial secondary metabolite with multiple bioactivities; however, the antiviral activity has not been reported yet. In the present study, we found the antiviral activity of bacterial prodigiosin on Bombyx mori nucleopolyhedrovirus (BmNPV)-infected cells in vitro, with specific modes of action. Prodigiosin at nontoxic concentrations selectively killed virus-infected cells, inhibited viral gene transcription, especially viral early gene ie-1, and prevented virus-mediated membrane fusion. Under prodigiosin treatment, both progeny virus production and viral DNA replication were significantly inhibited. Fluorescent assays showed that prodigiosin predominantly located in cytoplasm which suggested it might interact with cytoplasm factors to inhibit virus replication. In conclusion, the present study clearly indicates that prodigiosin possesses significant antiviral activity against BmNPV.
authors.affiliation: Laboratory of Invertebrate Pathology and Applied Microbiology, College of Biotechnology, Southwest University, Chongqing, 400716, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhou
authors.affiliation: Laboratory of Invertebrate Pathology and Applied Microbiology, College of Biotechnology, Southwest University, Chongqing, 400716, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Zeng
authors.affiliation: Laboratory of Invertebrate Pathology and Applied Microbiology, College of Biotechnology, Southwest University, Chongqing, 400716, China.
authors.forename: RenHua
authors.initials: R
authors.lastname: Liu
authors.affiliation: Laboratory of Invertebrate Pathology and Applied Microbiology, College of Biotechnology, Southwest University, Chongqing, 400716, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
authors.affiliation: Laboratory of Invertebrate Pathology and Applied Microbiology, College of Biotechnology, Southwest University, Chongqing, 400716, China.
authors.forename: Ru
authors.initials: R
authors.lastname: Li
authors.affiliation: Laboratory of Invertebrate Pathology and Applied Microbiology, College of Biotechnology, Southwest University, Chongqing, 400716, China.
authors.forename: XinYan
authors.initials: X
authors.lastname: Wang
authors.affiliation: Laboratory of Invertebrate Pathology and Applied Microbiology, College of Biotechnology, Southwest University, Chongqing, 400716, China.
authors.forename: WenWen
authors.initials: W
authors.lastname: Bai
authors.affiliation: Laboratory of Invertebrate Pathology and Applied Microbiology, College of Biotechnology, Southwest University, Chongqing, 400716, China.
authors.forename: XiaoYuan
authors.initials: X
authors.lastname: Liu
authors.affiliation: Laboratory of Invertebrate Pathology and Applied Microbiology, College of Biotechnology, Southwest University, Chongqing, 400716, China.
authors.forename: TingTing
authors.initials: T
authors.lastname: Xiang
authors.affiliation: Laboratory of Invertebrate Pathology and Applied Microbiology, College of Biotechnology, Southwest University, Chongqing, 400716, China.|College of Animal Science and Technology, Southwest University, Chongqing, 400716, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Laboratory of Invertebrate Pathology and Applied Microbiology, College of Biotechnology, Southwest University, Chongqing, 400716, China. canbl3312@126.com.
authors.forename: YongJi
authors.initials: Y
authors.lastname: Wan
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D012831:Bombyx; D002460:Cell Line; D017924:Nucleopolyhedroviruses / Q000187:drug effects*; D011353:Prodigiosin / Q000494:pharmacology*; D014779:Virus Replication / Q000187:drug effects
chemical_list: D000998:Antiviral Agents; D011353:Prodigiosin
keywords: Antiviral activity; Bombyx mori nucleopolyhedrovirus; Prodigiosin; Silkworm