title: [Effect of enhanced green fluorescent protein fusion on Ano1 physiological feature].
abstract: The aim of the present study was to investigate whether the physiological features of Ano1 were affected by enhanced green fluorescent protein (EGFP) fusing at Ano1 C-terminal. The eukaryotic expression vectors of Ano1 and EGFP-Ano1 were constructed, and these plasmids were transfected into Fischer rat thyroid follicular epithelial (FRT) cells using liposome. The expression and location of Ano1 were examined by using inverted fluorescence microscope. The ability of Ano1 to transport iodide was detected by kinetics experiment of fluorescence quenching. The results showed that both Ano1 and EGFP-Ano1 were expressed on FRT cell membrane and could be activated by Ca(2+). There was no significant difference of the ability to transport iodide between Ano1 and EGFP-Ano1. These results suggest Ano1 and EGFP-Ano1 have similar physiological feature.
authors.affiliation: Department of Biochemistry Laboratory, Jilin Medical College, Jilin 132013, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Zheng
authors.affiliation: Department of Biochemistry Laboratory, Jilin Medical College, Jilin 132013, China.
authors.forename: Hui-Jing
authors.initials: HJ
authors.lastname: Xu
authors.affiliation: Department of Hematology, Jilin Medical College, Jilin 132013, China.
authors.forename: Yu-Xuan
authors.initials: YX
authors.lastname: Zang
authors.affiliation: Department of Biochemistry Laboratory, Jilin Medical College, Jilin 132013, China.
authors.forename: Yi-Ju
authors.initials: YJ
authors.lastname: Hou
authors.affiliation: Department of Biochemistry Laboratory, Jilin Medical College, Jilin 132013, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Biochemistry Laboratory, Jilin Medical College, Jilin 132013, China.
authors.forename: Hai-Ou
authors.initials: HO
authors.lastname: Yang
authors.affiliation: Department of Hematology, Jilin Medical College, Jilin 132013, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Department of Hematology, Jilin Medical College, Jilin 132013, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Fang
authors.affiliation: Department of Biochemistry Laboratory, Jilin Medical College, Jilin 132013, China. haof863@126.com.
authors.forename: Feng
authors.initials: F
authors.lastname: Hao
mesh_terms: D000818:Animals; D000075369:Anoctamin-1; D002462:Cell Membrane / Q000502:physiology; D018118:Chloride Channels / Q000378:metabolism*; D004847:Epithelial Cells / Q000502:physiology*; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D010957:Plasmids; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D013961:Thyroid Gland / Q000166:cytology*; D014162:Transfection
chemical_list: C552942:ANO1 protein, rat; D000075369:Anoctamin-1; D018118:Chloride Channels; D011993:Recombinant Fusion Proteins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins