title: 3D cell culture to determine in vitro biocompatibility of bioactive glass in association with chitosan.
abstract: This study reports the in vitro biocompatibility of a composite biomaterial composed of 46S6 bioactive glass in association with chitosan (CH) by using 3D osteoblast culture of SaOS2. The 46S6 and CH composite (46S6-CH) forms small hydroxyapatite crystals on its surface after only three days immersion in the simulated body fluid. For 2D osteoblast culture, a significant increase in cell proliferation was observed after three days of contact with 46S6 or 46S6-CH-immersed media. After six days, 46S6-CH led to a significant increase in cell proliferation (128%) compared with pure 46S6 (113%) and pure CH (122%). For 3D osteoblast culture, after six days of culture, there was an increase in gene expression of markers of the early osteoblastic differentiation (RUNX2, ALP, COL1A1). Geometric structures corresponding to small apatite clusters were observed by SEM on the surface of the spheroids cultivated with 46S6 or 46S6-CH-immersed media. We showed different cellular responses depending on the 2D and 3D cell culture model. The induction of osteoblast differentiation in the 3D cell culture explained the differences of cell proliferation in contact with 46S6, CH or 46S6-CH-immersed media. This study confirmed that the 3D cell culture model is a very promising tool for in vitro biological evaluation of bone substitutes' properties.
authors.affiliation: Equipe Chimie du Solide et Matériaux - UMR CNRS 6226 - Sciences Chimiques de Rennes, Université de Rennes 1, Université Européenne de Bretagne, Campus de Beaulieu, 263 avenue du Général Leclerc, 35042 Rennes Cedex, France.|CHU de Rennes, Pôle d'Odontologie et de Chirurgie Buccale, 2 place Pasteur, 35000 Rennes, France.
authors.forename: Y
authors.initials: Y
authors.lastname: Bédouin
authors.affiliation: Equipe Chimie du Solide et Matériaux - UMR CNRS 6226 - Sciences Chimiques de Rennes, Université de Rennes 1, Université Européenne de Bretagne, Campus de Beaulieu, 263 avenue du Général Leclerc, 35042 Rennes Cedex, France.
authors.forename: P
authors.initials: P
authors.lastname: Pellen Mussi
authors.affiliation: Equipe Chimie du Solide et Matériaux - UMR CNRS 6226 - Sciences Chimiques de Rennes, Université de Rennes 1, Université Européenne de Bretagne, Campus de Beaulieu, 263 avenue du Général Leclerc, 35042 Rennes Cedex, France.
authors.forename: S
authors.initials: S
authors.lastname: Tricot-Doleux
authors.affiliation: Equipe Chimie du Solide et Matériaux - UMR CNRS 6226 - Sciences Chimiques de Rennes, Université de Rennes 1, Université Européenne de Bretagne, Campus de Beaulieu, 263 avenue du Général Leclerc, 35042 Rennes Cedex, France.|CHU de Rennes, Pôle d'Odontologie et de Chirurgie Buccale, 2 place Pasteur, 35000 Rennes, France.
authors.forename: D
authors.initials: D
authors.lastname: Chauvel-Lebret
authors.affiliation: Faculté de Chirurgie dentaire, Université d'Auvergne, 11 Boulevard Charles de Gaulle, 63000 Clermont-Ferrand, France.|CHU de Clermont-Ferrand, service d'Odontologie, 11 rue Léon Malfreyt, 63000 Clermont-Ferrand, France.
authors.forename: P
authors.initials: P
authors.lastname: Auroy
authors.affiliation: CHU de Rennes, Pôle d'Odontologie et de Chirurgie Buccale, 2 place Pasteur, 35000 Rennes, France.
authors.forename: X
authors.initials: X
authors.lastname: Ravalec
authors.affiliation: Equipe Chimie du Solide et Matériaux - UMR CNRS 6226 - Sciences Chimiques de Rennes, Université de Rennes 1, Université Européenne de Bretagne, Campus de Beaulieu, 263 avenue du Général Leclerc, 35042 Rennes Cedex, France.
authors.forename: H
authors.initials: H
authors.lastname: Oudadesse
authors.affiliation: Equipe Chimie du Solide et Matériaux - UMR CNRS 6226 - Sciences Chimiques de Rennes, Université de Rennes 1, Université Européenne de Bretagne, Campus de Beaulieu, 263 avenue du Général Leclerc, 35042 Rennes Cedex, France.|CHU de Nantes, service d'Odontologie, 1 Place Alexis Ricordeau, 44000 Nantes, France.
authors.forename: F
authors.initials: F
authors.lastname: Perez
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D018786:Bone Substitutes / Q000737:chemistry; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D055598:Chemical Phenomena; D048271:Chitosan / Q000737:chemistry*; D017886:Durapatite / Q000737:chemistry; D005898:Glass / Q000737:chemistry*; D006801:Humans; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects; D010012:Osteogenesis / Q000187:drug effects
chemical_list: D001672:Biocompatible Materials; D018786:Bone Substitutes; D048271:Chitosan; D017886:Durapatite
keywords: Spheroids; bioactive glass; biocompatibility; chitosan