title: Analysis of the gene polymorphism of aldosterone synthase (CYP11B2) and atrial natriuretic peptide (ANP) in women with preeclampsia.
abstract: OBJECTIVE
  Preeclampsia (PE) is a major cause of mortality of mothers, fetuses and newborns around the world. The etiology of preeclampsia has not yet been clarified, but many studies indicate a multifactorial basis of PE. Aldosterone synthase (CYP11B2) is responsible for synthesis of aldosterone responsible for regulating blood pressure. Similarly, natriuretic peptide (ANP) regulates blood pressure through a variety of mechanisms affecting the sodium concentration and the amount of extracellular fluid. Currently, attention is paid to the role of the polymorphisms in the expression level of these genes. The aim of the study was to determine the frequencies of genotypes and alleles for polymorphisms of -344C>T CYP11B2 gene and 2238T>C ANP gene in women with preeclampsia and healthy pregnant women from the Caucasian population.
  
  
  METHODS
  The study included a group of 165 pregnant women (59 women with preeclampsia and 109 healthy pregnant women). DNA was extracted from peripheral blood. Determination of the polymorphism of -344C>T CYP11B2 gene and 2238T>C ANP gene was performed by PCR-RFLP method.
  
  
  RESULTS
  The results showed that the frequencies of the TC and CC genotypes of 2238T>C polymorphism in ANP gene were significantly higher in patients with PE compared to control group. For -344C>T polymorphism of CYP11B2 gene, the frequency of TT genotype was significantly higher in patients with hypertension than in controls (32.2% vs. 23.58%).
  
  
  CONCLUSIONS
  Our findings showed that gene polymorphism of CYP11B2 (-344C>T) and ANP (2238T>C) may be associated with developing PE during pregnancy.
authors.affiliation: Laboratory of Experimental Pharmacogenetics, Department of Clinical Pharmacy and Biopharmacy, University of Medical Sciences, Poznan, Poland; Department of Stem Cells and Regenerative Medicine, Institute of Natural Fibres and Medicinal Plants, Poznan, Poland. Electronic address: aniabogacz23@o2.pl.
authors.forename: Anna
authors.initials: A
authors.lastname: Bogacz
authors.affiliation: Laboratory of Experimental Pharmacogenetics, Department of Clinical Pharmacy and Biopharmacy, University of Medical Sciences, Poznan, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Bartkowiak-Wieczorek
authors.affiliation: Department of Laboratory Diagnostics, Greater Poland Cancer Centre, Poznan, Poland.
authors.forename: Danuta
authors.initials: D
authors.lastname: Procyk
authors.affiliation: Division of Perinatology and Women's Diseases, Poznan University of Medical Sciences, Poznan, Poland; Department of Pharmacology and Phytochemistry, Institute of Natural Fibres and Medicinal Plants, Poznan, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Seremak-Mrozikiewicz
authors.affiliation: Department of Rehabilitation, Poznan University of Medical Sciences, Poznan, Poland.
authors.forename: Marian
authors.initials: M
authors.lastname: Majchrzycki
authors.affiliation: Department of Stem Cells and Regenerative Medicine, Institute of Natural Fibres and Medicinal Plants, Poznan, Poland.
authors.forename: Karolina
authors.initials: K
authors.lastname: Dziekan
authors.affiliation: Laboratory of Experimental Pharmacogenetics, Department of Clinical Pharmacy and Biopharmacy, University of Medical Sciences, Poznan, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Bienert
authors.affiliation: Department of Stem Cells and Regenerative Medicine, Institute of Natural Fibres and Medicinal Plants, Poznan, Poland; Department of General Pharmacology and Pharmacoeconomics, Pomeranian Medical University, Szczecin, Poland.
authors.forename: Bogusław
authors.initials: B
authors.lastname: Czerny
mesh_terms: D000328:Adult; D009320:Atrial Natriuretic Factor / Q000235:genetics*; D001794:Blood Pressure / Q000235:genetics*; D001835:Body Weight; D016022:Case-Control Studies; D019405:Cytochrome P-450 CYP11B2 / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D046110:Hypertension, Pregnancy-Induced / Q000235:genetics; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D011225:Pre-Eclampsia / Q000235:genetics*; D011247:Pregnancy; D044465:White People / Q000235:genetics
chemical_list: D009320:Atrial Natriuretic Factor; D019405:Cytochrome P-450 CYP11B2
keywords: Aldosterone synthase; Natriuretic peptide; Polymorphism; Preeclampsia