title: Genome-Wide Dynamic Profiling of Histone Methylation during Nuclear Transfer-Mediated Porcine Somatic Cell Reprogramming.
abstract: The low full-term developmental efficiency of porcine somatic cell nuclear transfer (SCNT) embryos is mainly attributed to imperfect epigenetic reprogramming in the early embryos. However, dynamic expression patterns of histone methylation involved in epigenetic reprogramming progression during porcine SCNT embryo early development remain to be unknown. In this study, we characterized and compared the expression patterns of multiple histone methylation markers including transcriptionally repressive (H3K9me2, H3K9me3, H3K27me2, H3K27me3, H4K20me2 and H4K20me3) and active modifications (H3K4me2, H3K4me3, H3K36me2, H3K36me3, H3K79me2 and H3K79me3) in SCNT early embryos from different developmental stages with that from in vitro fertilization (IVF) counterparts. We found that the expression level of H3K9me2, H3K9me3 and H4K20me3 of SCNT embryos from 1-cell to 4-cell stages was significantly higher than that in the IVF embryos. We also detected a symmetric distribution pattern of H3K9me2 between inner cell mass (ICM) and trophectoderm (TE) in SCNT blastocysts. The expression level of H3K9me2 in both lineages from SCNT expanded blastocyst onwards was significantly higher than that in IVF counterparts. The expression level of H4K20me2 was significantly lower in SCNT embryos from morula to blastocyst stage compared with IVF embryos. However, no aberrant dynamic reprogramming of H3K27me2/3 occurred during early developmental stages of SCNT embryos. The expression of H3K4me3 was higher in SCNT embryos at 4-cell stage than that of IVF embryos. H3K4me2 expression in SCNT embryos from 8-cell stage to blastocyst stage was lower than that in the IVF embryos. Dynamic patterns of other active histone methylation markers were similar between SCNT and IVF embryos. Taken together, histone methylation exhibited developmentally stage-specific abnormal expression patterns in porcine SCNT early embryos.
authors.affiliation: Anhui Provincial Laboratory of Local Livestock and Poultry Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei City, Anhui Province, China.|State Key Laboratory for Agrobiotechnology, College of Biological Science, China Agricultural University, Haidian District, Beijing, China.
authors.forename: Zubing
authors.initials: Z
authors.lastname: Cao
authors.affiliation: Anhui Provincial Laboratory of Local Livestock and Poultry Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei City, Anhui Province, China.
authors.forename: Yunsheng
authors.initials: Y
authors.lastname: Li
authors.affiliation: Anhui Provincial Laboratory of Local Livestock and Poultry Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei City, Anhui Province, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Anhui Provincial Laboratory of Local Livestock and Poultry Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei City, Anhui Province, China.
authors.forename: Heng
authors.initials: H
authors.lastname: Wang
authors.affiliation: Anhui Provincial Laboratory of Local Livestock and Poultry Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei City, Anhui Province, China.
authors.forename: Meiling
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Anhui Provincial Laboratory of Local Livestock and Poultry Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei City, Anhui Province, China.
authors.forename: Naru
authors.initials: N
authors.lastname: Zhou
authors.affiliation: Anhui Provincial Laboratory of Local Livestock and Poultry Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei City, Anhui Province, China.
authors.forename: Ronghua
authors.initials: R
authors.lastname: Wu
authors.affiliation: Anhui Provincial Laboratory of Local Livestock and Poultry Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei City, Anhui Province, China.
authors.forename: Yinghui
authors.initials: Y
authors.lastname: Ling
authors.affiliation: Anhui Provincial Laboratory of Local Livestock and Poultry Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei City, Anhui Province, China.
authors.forename: Fugui
authors.initials: F
authors.lastname: Fang
authors.affiliation: State Key Laboratory for Agrobiotechnology, College of Biological Science, China Agricultural University, Haidian District, Beijing, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.affiliation: Anhui Provincial Laboratory of Local Livestock and Poultry Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei City, Anhui Province, China.
authors.forename: Yunhai
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D053624:Blastocyst Inner Cell Mass / Q000378:metabolism; D000067470:Cellular Reprogramming Techniques / Q000662:veterinary*; D046149:Embryo Culture Techniques; D047108:Embryonic Development; D044127:Epigenesis, Genetic; D018507:Gene Expression Regulation, Developmental; D006657:Histones / Q000378:metabolism*; D008297:Male; D008745:Methylation; D053652:Nuclear Transfer Techniques / Q000662:veterinary*; D013552:Swine / Q000196:embryology* / Q000378:metabolism; D014327:Trophoblasts / Q000378:metabolism
chemical_list: D006657:Histones