title: [PROTECTION EFFECTS OF BONE MARROW MESENCHYMAL STEM CELLS PARACRINE ON CHONDROCYTES INJURED BY INTERLEUKIN 1β].
abstract: OBJECTIVE
  To study the potential protective effects of bone marrow mesenchymal stem cells (BMSCs) on chondrocytes injured by interleukin 1β (IL-1β), and the resistant capacity of chondrocytes when co-cultured indirectly with BMSCs against IL-1β.
  
  
  METHODS
  Six Sprague Dawley (SD) rats were randomly divided into experimental group (articular cartilage defects) and control group. The content and gene expression of IL-1β were detected at 6 hours after surgical intervention by quantitative real time RCR (qRT-PCR) and ELISA. BMSCs repairing function test: the 18-holes cultured chondrocytes were randomly divided into 3 groups (n = 6): cells of blank group were not treated; cells of injured group and co-cultured group were intervened by IL-1β, and Transwell chamber was used to establish co-culture system of BMSCs with chondrocyte in co-cultured group. The mRNA relative expressions of cysteinyl aspartate specific proteinase 3 (Caspase 3), a disintegrin and metalloprotease with Thrombospondin motifs 4 (ADAMTS-4), and ADAMTS-5 were measured via qRT-PCR in chondrocytes, meanwhile Caspase-3 content was detected via ELISA, and the cell apoptosis rate was detected via flow cytometry. BMSCs protecting function test: the 12-holes cultured chondrocytes were randomly divided into 2 groups (n=6), Transwell chamber was used to establish co-culture system of BMSCs with chondrocyte in co-cultured group before the 2 groups were both intervened by IL-1β, then the same detected indexes were taken as the BMSCs repairing function test.
  
  
  RESULTS
  Animal in vivo studies showed that relative expression of IL-1β mRNA and IL-1β contents were significantly higher in experimental group than control group (P < 0.05). BMSCs repair tests showed that mRNA relative expressions of Caspase-3, ADAMTS-4, and ADAMTS-5, Caspase-3 content, and cell apoptosis rate were significantly higher in injured group and co-cultured group than blank group, and in injured group than co-cultured group (P < 0.05). BMSCs protect tests showed that mRNA relative expressions of Caspase-3, ADAMTS-4, and ADAMTS-5, Caspase-3 content, and cell apoptosis rate in co-cultured group were significantly lower than those in control group (P < 0.05).
  
  
  CONCLUSIONS
  BMSCs, as seed cells for tissue engineering, have potential for applications to anti-inflammation and anti-apoptosis.
authors.forename: Lizhang
authors.initials: L
authors.lastname: Xu
authors.forename: Guoying
authors.initials: G
authors.lastname: Deng
authors.forename: Weiheng
authors.initials: W
authors.lastname: Wang
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Huang
authors.forename: Xiangqun
authors.initials: X
authors.lastname: Yang
authors.forename: Xiaojian
authors.initials: X
authors.lastname: Ye
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000378:metabolism*; D002358:Cartilage, Articular; D053148:Caspase 3 / Q000187:drug effects / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D019902:Chondrocytes / Q000187:drug effects*; D020219:Chondrogenesis / Q000187:drug effects* / Q000502:physiology; D018920:Coculture Techniques; D006412:Hematopoietic Stem Cells; D053583:Interleukin-1beta / Q000378:metabolism* / Q000494:pharmacology*; D059630:Mesenchymal Stem Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D023822:Tissue Engineering
chemical_list: D053583:Interleukin-1beta; D053148:Caspase 3