title: Molecular characterization of melanin-concentrating hormone (MCH) in Schizothorax prenanti: cloning, tissue distribution and role in food intake regulation.
abstract: Melanin-concentrating hormone (MCH) is a crucial neuropeptide involved in various biological functions in both mammals and fish. In this study, the full-length MCH cDNA was obtained from Schizothorax prenanti by rapid amplification of cDNA ends polymerase chain reaction. The full-length MCH cDNA contained 589 nucleotides including an open reading frame of 375 nucleotides encoding 256 amino acids. MCH mRNA was highly expressed in the brain by real-time quantitative PCR analysis. Within the brain, expression of MCH mRNA was preponderantly detected in the hypothalamus. In addition, the MCH mRNA expression in the S. prenanti hypothalamus of fed group was significantly decreased compared with the fasted group at 1 and 3 h post-feeding, respectively. Furthermore, the MCH gene expression presented significant increase in the hypothalamus of fasted group compared with the fed group during long-term fasting. After re-feeding, there was a dramatic decrease in MCH mRNA expression in the hypothalamus of S. prenanti. The results indicate that the expression of MCH is affected by feeding status. Taken together, our results suggest that MCH may be involved in food intake regulation in S. prenanti.
authors.affiliation: Department of Aquaculture, Sichuan Agricultural University, 46# Xinkang Road, Ya'an, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.affiliation: Department of Aquaculture, Sichuan Agricultural University, 46# Xinkang Road, Ya'an, China.
authors.forename: Dengyue
authors.initials: D
authors.lastname: Yuan
authors.affiliation: Department of Aquaculture, Sichuan Agricultural University, 46# Xinkang Road, Ya'an, China.
authors.forename: Chaowei
authors.initials: C
authors.lastname: Zhou
authors.affiliation: Department of Aquaculture, Sichuan Agricultural University, 46# Xinkang Road, Ya'an, China.
authors.forename: Fangjun
authors.initials: F
authors.lastname: Lin
authors.affiliation: Department of Aquaculture, Sichuan Agricultural University, 46# Xinkang Road, Ya'an, China.
authors.forename: Rongbin
authors.initials: R
authors.lastname: Wei
authors.affiliation: Department of Aquaculture, Sichuan Agricultural University, 46# Xinkang Road, Ya'an, China.
authors.forename: Hu
authors.initials: H
authors.lastname: Chen
authors.affiliation: Department of Aquaculture, Sichuan Agricultural University, 46# Xinkang Road, Ya'an, China.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Wu
authors.affiliation: Department of Aquaculture, Sichuan Agricultural University, 46# Xinkang Road, Ya'an, China.
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Xin
authors.affiliation: Department of Aquaculture, Sichuan Agricultural University, 46# Xinkang Road, Ya'an, China.
authors.forename: Ju
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Aquaculture, Sichuan Agricultural University, 46# Xinkang Road, Ya'an, China.
authors.forename: Yundi
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Department of Aquaculture, Sichuan Agricultural University, 46# Xinkang Road, Ya'an, China.
authors.forename: Defang
authors.initials: D
authors.lastname: Chen
authors.affiliation: Department of Aquaculture, Sichuan Agricultural University, 46# Xinkang Road, Ya'an, China.
authors.forename: Shiyong
authors.initials: S
authors.lastname: Yang
authors.affiliation: Department of Aquaculture, Sichuan Agricultural University, 46# Xinkang Road, Ya'an, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Aquaculture, Sichuan Agricultural University, 46# Xinkang Road, Ya'an, China.
authors.forename: Yundan
authors.initials: Y
authors.lastname: Pu
authors.affiliation: Department of Aquaculture, Sichuan Agricultural University, 46# Xinkang Road, Ya'an, China. lizhiqiong454@163.com.
authors.forename: Zhiqiong
authors.initials: Z
authors.lastname: Li
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003530:Cyprinidae / Q000235:genetics / Q000502:physiology; D018076:DNA, Complementary / Q000235:genetics; D004435:Eating / Q000235:genetics*; D005215:Fasting / Q000502:physiology*; D005260:Female; D029941:Fish Proteins / Q000235:genetics / Q000502:physiology; D007028:Hypothalamic Hormones / Q000235:genetics / Q000502:physiology; D007031:Hypothalamus / Q000378:metabolism; D008297:Male; D008543:Melanins / Q000235:genetics / Q000502:physiology; D010907:Pituitary Hormones / Q000235:genetics / Q000502:physiology; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D029941:Fish Proteins; D007028:Hypothalamic Hormones; D008543:Melanins; D010907:Pituitary Hormones; D012333:RNA, Messenger; C019463:melanin-concentrating hormone
keywords: Cloning; Fasting; MCH; Schizothorax prenanti; Tissue distribution