title: Tissue-specific patterns of gene expression in the epithelium and stroma of normal colon in healthy individuals in an aspirin intervention trial.
abstract: Regular aspirin use reduces colon adenoma and carcinoma incidence. UDP-glucuronosyltransferases (UGT) are involved in aspirin metabolism and clearance, and variant alleles in UGT1A6 have been shown to alter salicylic acid metabolism and risk of colon neoplasia. In a randomized, cross-over, placebo-controlled trial of 44 healthy men and women, homozygous for UGT1A6*1 or UGT1A6*2, we explored differences between global epithelial and stromal expression, using Affymetrix U133 + 2.0 microarrays and tested effects of 60-day aspirin supplementation (325 mg/d) on epithelial and stromal gene expression and colon prostaglandin E2 (PGE2) levels. We conducted a comprehensive study of differential gene expression between normal human colonic epithelium and stroma from healthy individuals. Although no statistically significant differences in gene expression were observed in response to aspirin or UGT1A6 genotype, we have identified the genes uniquely and reproducibly expressed in each tissue type and have analyzed the biologic processes they represent. Here we describe in detail how the data, deposited in the Gene Expression Omnibus (GEO) - accession number GSE71571 - was generated including the basic analysis as contained in the manuscript published in BMC Medical Genetics with the PMID 25927723 (Thomas et al., 2015 [9]).
authors.affiliation: Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA.
authors.forename: Sushma S
authors.initials: SS
authors.lastname: Thomas
authors.affiliation: Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA.
authors.forename: Karen W
authors.initials: KW
authors.lastname: Makar
authors.affiliation: Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA.
authors.forename: Lin
authors.initials: L
authors.lastname: Li
authors.affiliation: Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA.
authors.forename: Yingye
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: M.D. Anderson Cancer Center, Houston, TX 77030, USA.
authors.forename: Peiying
authors.initials: P
authors.lastname: Yang
authors.affiliation: Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA.
authors.forename: Lisa
authors.initials: L
authors.lastname: Levy
authors.affiliation: Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA.
authors.forename: Rebecca Y
authors.initials: RY
authors.lastname: Rudolph
authors.affiliation: Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA.
authors.forename: Paul D
authors.initials: PD
authors.lastname: Lampe
authors.affiliation: Case Western Reserve University School of Medicine, Cincinnati, OH 44106, USA.
authors.forename: Min
authors.initials: M
authors.lastname: Yan
authors.affiliation: Case Western Reserve University School of Medicine, Cincinnati, OH 44106, USA.
authors.forename: Sanford D
authors.initials: SD
authors.lastname: Markowitz
authors.affiliation: Amgen Corporation, Seattle, WA 98119, USA.
authors.forename: Jeannette
authors.initials: J
authors.lastname: Bigler
authors.affiliation: Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA.
authors.forename: Johanna W
authors.initials: JW
authors.lastname: Lampe
authors.affiliation: Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA.
authors.forename: John D
authors.initials: JD
authors.lastname: Potter