title: miRNA and target gene expression in menstrual endometria and early pregnancy decidua.
abstract: OBJECTIVE
  The role of miRNAs in modulating gene expression in decidualization remains to be determined. We performed a comparative study to identify miRNAs and their potential mRNA targets with different expression levels between endometrium and decidua.
  
  
  METHODS
  Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was used to measure the expression of the miR-146b-5p, miR-181b-5p, miR-424, miR-532, miR-199a-3p, miR-423, miR-22-3p, let-7i-5p, and miR-1 and the predicted target genes IGF2R, LEPR, SGK1, MMP2, MMP10, LIF, IL6, and STAT3 in menstrual endometria and early pregnancy decidua.
  
  
  RESULTS
  miR-146b-5p, miR-181b-5p, miR-424, miR-532, and miR-199a-3p were significantly downregulated in early pregnancy decidua, while miR-423, miR-22-3p, let-7i-5p, and miR-1 were significantly upregulated. In addition, the decidua had significantly lower levels of expression of LIF, IL6, MMP2, MMP10, and IGF2R and higher levels of expression of SGK1, LEPR, PROK1, and STAT3 than the menstrual endometria group.
  
  
  CONCLUSIONS
  Our results provide new insights into the expression of miRNAs that regulate genes involved in decidualization and the maintenance of early pregnancy.
authors.affiliation: The Key Laboratory of National Education Ministry for Mammalian Reproductive Biology and Biotechnology, Inner Mongolia University, Hohhot 010070, Inner Mongolia, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Lv
authors.affiliation: School of Life Sciences, Inner Mongolia University, Hohhot 010021, Inner Mongolia, China.
authors.forename: Shujun
authors.initials: S
authors.lastname: Gao
authors.affiliation: School of Life Sciences, Inner Mongolia University, Hohhot 010021, Inner Mongolia, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Center for Reproductive Medicine, The Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010059, Inner Mongolia, China.
authors.forename: Liyan
authors.initials: L
authors.lastname: Wang
authors.affiliation: Center for Reproductive Medicine, The Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010059, Inner Mongolia, China. Electronic address: 425echo@163.com.
authors.forename: Xiujuan
authors.initials: X
authors.lastname: Chen
authors.affiliation: Center for Reproductive Medicine, The Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010059, Inner Mongolia, China. Electronic address: wuai1544@163.com.
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000328:Adult; D003656:Decidua / Q000378:metabolism*; D015536:Down-Regulation; D004717:Endometrium / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D017874:Immediate-Early Proteins / Q000235:genetics; D015850:Interleukin-6 / Q000235:genetics; D053605:Leukemia Inhibitory Factor / Q000235:genetics; D053506:Matrix Metalloproteinase 10 / Q000235:genetics; D020778:Matrix Metalloproteinase 2 / Q000235:genetics; D008598:Menstruation / Q000235:genetics* / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D011247:Pregnancy / Q000235:genetics* / Q000378:metabolism; D011261:Pregnancy Trimester, First / Q000235:genetics; D017346:Protein Serine-Threonine Kinases / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism*; D017527:Receptor, IGF Type 2 / Q000235:genetics; D054411:Receptors, Leptin / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D050796:STAT3 Transcription Factor / Q000235:genetics; D015854:Up-Regulation; D055815:Young Adult; D000099184:Serum-Glucocorticoid Regulated Kinases
chemical_list: D017874:Immediate-Early Proteins; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; D053506:Matrix Metalloproteinase 10; D020778:Matrix Metalloproteinase 2; D035683:MicroRNAs; D017346:Protein Serine-Threonine Kinases; D012333:RNA, Messenger; D017527:Receptor, IGF Type 2; D054411:Receptors, Leptin; D050796:STAT3 Transcription Factor; D000099184:Serum-Glucocorticoid Regulated Kinases; C508600:IL6 protein, human; C515448:LEPR protein, human; C507837:LIF protein, human; C494086:STAT3 protein, human; C511770:MMP10 protein, human; C522361:MMP2 protein, human
keywords: Decidua; Menstrual endometrium; MicroRNA; Target gene