title: Recovery of polyphenols from red grape pomace and assessment of their antioxidant and anti-cholesterol activities.
abstract: The present work aimed at the recovery and characterization of polyphenolic compounds extracted from red grape pomace (Vitis vinifera L.), a winemaking by-product. Polyphenolic compounds of wet (WP) and dried (DP) red pomace were recovered by enzymatic digestions and ethanol-based extractions. Fungamyl and Celluclast enzymes were found to be the most effective in enhancing polyphenol release from WP. WP samples showed the highest capacity of releasing polyphenols with 2h control 24°C and 2h 1% Celluclast resulting as the best treatments. A significantly lower amount of polyphenols was recovered from DP most probably as a consequence of the pomace drying. The best extracts contained high amounts of total polyphenols, flavonoids, tannins and anthocyanins and exerted antioxidant and cholesterol-lowering activities. The results support the possibility of exploiting the extracts coming from grape processing by-products as ingredients for functional and innovative products in the nutraceutical, pharmaceutical or cosmetic fields.
authors.affiliation: Department of Biological, Geological and Environmental Sciences, University of Bologna, via Irnerio 42, 40126 Bologna, Italy; Department of Civil, Chemical, Environmental and Materials Engineering, University of Bologna, via Terracini 28, 40131 Bologna, Italy.
authors.forename: Maura
authors.initials: M
authors.lastname: Ferri
authors.affiliation: Department of Biological, Geological and Environmental Sciences, University of Bologna, via Irnerio 42, 40126 Bologna, Italy.
authors.forename: Sofia
authors.initials: S
authors.lastname: Bin
authors.affiliation: Eridania Sadam Spa, via Agresti 6, 40123 Bologna, Italy.
authors.forename: Veronica
authors.initials: V
authors.lastname: Vallini
authors.affiliation: Department of Civil, Chemical, Environmental and Materials Engineering, University of Bologna, via Terracini 28, 40131 Bologna, Italy.
authors.forename: Fabio
authors.initials: F
authors.lastname: Fava
authors.affiliation: Department of Chemistry G. Ciamician, University of Bologna, Via Selmi 2, 40126 Bologna, Italy.
authors.forename: Elisa
authors.initials: E
authors.lastname: Michelini
authors.affiliation: Department of Chemistry G. Ciamician, University of Bologna, Via Selmi 2, 40126 Bologna, Italy.
authors.forename: Aldo
authors.initials: A
authors.lastname: Roda
authors.affiliation: Sadam Engineering Srl, via Agresti 6, 40123 Bologna, Italy.
authors.forename: Giordano
authors.initials: G
authors.lastname: Minnucci
authors.affiliation: Sebigas Spa, via Agresti 6, 40123 Bologna, Italy.
authors.forename: Giacomo
authors.initials: G
authors.lastname: Bucchi
authors.affiliation: Department of Biological, Geological and Environmental Sciences, University of Bologna, via Irnerio 42, 40126 Bologna, Italy. Electronic address: annalisa.tassoni2@unibo.it.
authors.forename: Annalisa
authors.initials: A
authors.lastname: Tassoni
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D002784:Cholesterol / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D004798:Enzymes / Q000378:metabolism; D000431:Ethanol / Q000737:chemistry; D005419:Flavonoids / Q000032:analysis; D010936:Plant Extracts / Q000737:chemistry; D059808:Polyphenols / Q000302:isolation & purification*; D013634:Tannins / Q000032:analysis; D027843:Vitis / Q000737:chemistry*; D014866:Waste Products / Q000032:analysis*; D014867:Water / Q000737:chemistry
chemical_list: D000975:Antioxidants; D004798:Enzymes; D005419:Flavonoids; D010936:Plant Extracts; D059808:Polyphenols; D013634:Tannins; D014866:Waste Products; D014867:Water; D000431:Ethanol; D002784:Cholesterol