title: Development and validation of SPMMTE HPLC method for analysis of profens from human plasma.
abstract: A fast, selective and reproducible solid-phase membrane microtip extraction (SPMMTE) HPLC method has been developed and validated for the analyses of ibuprofen, ketoprofen, and flurbiprofen from human plasma. The analysis was carried out on a C18 (150 × 4.6 mm; 5 μm) column. The mobile phase used was water-acetonitrile (55:45, v/v) adjusted to pH 3.0 using trifluoroacetic acid, at a flow rate 0.5 mL/min with a detection wavelength of 225 nm. The values for the capacity factors for the profen samples ranged from 0.47 to 1.50. The values for the selectivity factor (α) for ketoprofen-flurbiprofen, flurbiprofen-ibuprofen and ibuprofen-ketoprofen combinations from human plasma samples were 1.99, 1.00 and 2.10, respectively. The resolution factors (Rs) for ketoprofen-flurbiprofen, flurbiprofen-ibuprofen and ibuprofen-ketoprofen from plasma samples were 3.00, 1.50 and 4.10, respectively. The percentage recoveries of ibuprofen, ketoprofen, and flurbiprofen from human plasma were 75-85%. All of the profens were separated within 7.0 min, indicating a relatively fast method. During the development of the SPMMTE procedure the parameters of pH, contact time, desorption and types of solvents were optimized. The final method was also found to be efficient, effective and inexpensive. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: Department of Pharmacognosy, College of Pharmacy, King Saud University, PO Box 2457, Riyadh, 11451, Kingdom of Saudi Arabia.
authors.forename: Afzal
authors.initials: A
authors.lastname: Hussain
authors.affiliation: Department of Pharmacognosy, College of Pharmacy, King Saud University, PO Box 2457, Riyadh, 11451, Kingdom of Saudi Arabia.
authors.forename: Mohamed F
authors.initials: MF
authors.lastname: Al-Ajmi
authors.affiliation: Department of Chemistry, College of Science and General Studies, Alfaisal University, PO Box 50927, Riyadh, 11533, Saudi Arabia.
authors.forename: Samira
authors.initials: S
authors.lastname: Amir
authors.affiliation: Department of Chemistry, Jamia Millia Islamia, New Delhi, 110025, India.
authors.forename: Imran
authors.initials: I
authors.lastname: Ali
mesh_terms: D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000097:blood* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D015203:Reproducibility of Results; D052616:Solid Phase Extraction / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal
keywords: flurbiprofen; high-performance liquid chromatography; ibuprofen; ketoprofen; mechanism of separation; solid-phase membrane microtip extraction