title: Indicators of iron status are correlated with adiponectin expression in adipose tissue of patients with morbid obesity.
abstract: OBJECTIVE
  The aim of this study was to assess interactions between glucose and iron homoeostasis in the adipose tissue (AT) of obese subjects.
  
  
  METHODS
  A total of 46 obese patients eligible for bariatric surgery were recruited into the study. Anthropometric and biochemical characteristics were assessed, and biopsies of subcutaneous (SCAT) and visceral adipose tissue (VAT) performed. The mRNA levels of genes involved in iron and glucose homoeostasis were measured in their AT and compared with a pool of control samples.
  
  
  RESULTS
  Gene expression of hepcidin (HAMP) was significantly increased in the SCAT and VAT of obese patients, while transferrin receptor (TFRC) expression was reduced, compared with non-obese controls, suggesting a higher iron load in obese patients. Also, mRNA levels of adiponectin (ADIPOQ) were decreased in both SCAT and VAT in obese patients, and correlated negatively with hepcidin expression, while adiponectin expression was positively correlated with TFRC expression in both SCAT and VAT. Interestingly, TFRC expression in VAT correlated negatively with several metabolic parameters, such as fasting blood glucose and LDL cholesterol.
  
  
  CONCLUSIONS
  Iron content appears to be increased in the SCAT and VAT of obese patients, and negatively correlated with adiponectin expression, which could be contributing to insulin resistance and the metabolic complications of obesity.
authors.affiliation: Inserm, UMR 991, Université de Rennes 1, 35000 Rennes, France; Service d'Endocrinologie-Diabétologie, CHU de Rennes, 35200 Rennes, France.
authors.forename: F
authors.initials: F
authors.lastname: Pihan-Le Bars
authors.affiliation: Inserm, UMR 991, Université de Rennes 1, 35000 Rennes, France; Service d'Endocrinologie-Diabétologie, CHU de Rennes, 35200 Rennes, France. Electronic address: fabrice.bonnet@chu-rennes.fr.
authors.forename: F
authors.initials: F
authors.lastname: Bonnet
authors.affiliation: Inserm, UMR 991, Université de Rennes 1, 35000 Rennes, France; Centre National de Référence des surcharges en fer rares d'origine génétique, CHU Pontchaillou, 35000 Rennes, France.
authors.forename: O
authors.initials: O
authors.lastname: Loréal
authors.affiliation: Laboratoire de Biochimie, CHU de Nantes, 44000 Nantes, France; Université de Nantes, 44000 Nantes, France.
authors.forename: A-G
authors.initials: AG
authors.lastname: Le Loupp
authors.affiliation: Inserm, UMR 991, Université de Rennes 1, 35000 Rennes, France; Laboratoire de Biochimie, CHU de Rennes, 35000 Rennes, France.
authors.forename: M
authors.initials: M
authors.lastname: Ropert
authors.affiliation: Clinique de Chirurgie Digestive et Endocrinienne, CHU de Nantes, 44000 Nantes, France.
authors.forename: E
authors.initials: E
authors.lastname: Letessier
authors.affiliation: Université de Nantes, 44000 Nantes, France; Inserm, UMR 1087-CNRS UMR 6291, l'Institut du Thorax, 44000 Nantes, France.
authors.forename: X
authors.initials: X
authors.lastname: Prieur
authors.affiliation: Laboratoire de Biochimie, CHU de Nantes, 44000 Nantes, France; Université de Nantes, 44000 Nantes, France.
authors.forename: K
authors.initials: K
authors.lastname: Bach
authors.affiliation: Inserm, UMR 991, Université de Rennes 1, 35000 Rennes, France; Centre National de Référence des surcharges en fer rares d'origine génétique, CHU Pontchaillou, 35000 Rennes, France; Service des maladies du foie, CHU de Rennes, 35000 Rennes, France.
authors.forename: Y
authors.initials: Y
authors.lastname: Deugnier
authors.affiliation: Inserm, UMR 991, Université de Rennes 1, 35000 Rennes, France.
authors.forename: B
authors.initials: B
authors.lastname: Fromenty
authors.affiliation: Université de Nantes, 44000 Nantes, France; Inserm, UMR 1087-CNRS UMR 6291, l'Institut du Thorax, 44000 Nantes, France; CHU de Nantes, clinique d'Endocrinologie, l'Institut du Thorax, 44000 Nantes, France.
authors.forename: B
authors.initials: B
authors.lastname: Cariou
mesh_terms: D052242:Adiponectin / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D000273:Adipose Tissue / Q000737:chemistry / Q000378:metabolism*; D000328:Adult; D005260:Female; D064451:Hepcidins / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007333:Insulin Resistance; D007501:Iron / Q000097:blood*; D008297:Male; D008875:Middle Aged; D009767:Obesity, Morbid / Q000097:blood / Q000453:epidemiology / Q000378:metabolism*; D011446:Prospective Studies
chemical_list: D052242:Adiponectin; D064451:Hepcidins; D007501:Iron
keywords: Adiponectin; Adipose tissue; Hepcidin; Insulin resistance; Iron; Obesity