title: Regional brain dysregulation of Ca(2+)-handling systems in ketamine-induced rat model of experimental psychosis.
abstract: Chronic N-methyl-D-aspartate receptor (NMDAR) antagonist treatment can provide valuable neurochemical and neuroanatomical models of experimental psychosis. One such antagonist, ketamine, with its short half-time and well-documented psychotomimetic action, has cognitive effects resembling various aspects of schizophrenia-like symptoms. In order to obtain insights into possible relationships between Ca(2+) homeostasis and schizophrenia-related symptoms, we investigate the effects of chronic ketamine administration on intracellular Ca(2+) levels in various brain regions and on the expression level of key members of the neuronal Ca(2+)-handling system in rats. We show increased intracellular [Ca(2+)] in all of the examined brain regions following ketamine treatment but an altered cytosolic Ca(2+) level correlated with hyperlocomotor activity was only established for the cortex and striatum. Our findings also suggest that an imbalance in the expression between the calcium "on" and "off" systems contributes to the deregulation of brain Ca(2+) homeostasis in our ketamine-induced model of experimental psychosis. Identification of the genes whose expression is affected by ketamine treatment indicates their involvement as putative etiological factors in schizophrenia.
authors.affiliation: Department of Molecular Neurochemistry, Faculty of Health Sciences, Medical University of Lodz, 6/8 Mazowiecka Street, 92215, Lodz, Poland. malwina.lisek@umed.lodz.pl.
authors.forename: Malwina
authors.initials: M
authors.lastname: Lisek
authors.affiliation: Department of Molecular Neurochemistry, Faculty of Health Sciences, Medical University of Lodz, 6/8 Mazowiecka Street, 92215, Lodz, Poland.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Boczek
authors.affiliation: Department of Molecular Neurochemistry, Faculty of Health Sciences, Medical University of Lodz, 6/8 Mazowiecka Street, 92215, Lodz, Poland.
authors.forename: Bozena
authors.initials: B
authors.lastname: Ferenc
authors.affiliation: Department of Molecular Neurochemistry, Faculty of Health Sciences, Medical University of Lodz, 6/8 Mazowiecka Street, 92215, Lodz, Poland.
authors.forename: Ludmila
authors.initials: L
authors.lastname: Zylinska
mesh_terms: D000818:Animals; D001522:Behavior, Animal; D001921:Brain / Q000378:metabolism / Q000473:pathology; D002118:Calcium / Q000378:metabolism*; D002469:Cell Separation; D002470:Cell Survival; D004195:Disease Models, Animal; D005786:Gene Expression Regulation; D006706:Homeostasis; D042541:Intracellular Space / Q000378:metabolism; D007649:Ketamine; D008297:Male; D009043:Motor Activity; D011618:Psychotic Disorders / Q000378:metabolism* / Q000473:pathology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D012333:RNA, Messenger; D007649:Ketamine; D002118:Calcium
keywords: Calcium homeostasis; Experimental psychosis; Ketamine; NMDA receptor; Rat