title: Biophysicochemical characterization of Pyocin SA189 produced by Pseudomonas aeruginosa SA189.
abstract: Pseudomonas aeruginosa, in spite of being a ubiquitous organism (as it is found in soil, water, and humans), is also an opportunistic pathogen. In order to maintain its diversity in the community, it produces various toxic proteins, known as, bacteriocins. In the present study, pyocin SA189, which is a bacteriocin produced by P. aeruginosa SA189 (isolated from a clinical sample) was characterized. P. aeruginosa SA189, as identified by the conventional and 16S rRNA gene amplification, produced pyocin SA189 of molecular weight of 66 k Da. The pyocin showed antimicrobial activity against several clinically relevant Gram-positive and Gram-negative bacteria and was substantially stable for wide ranges of temperature and pH. Furthermore, the pyocin also retained its biological activity upon treatment with metal ions, organic solvents, and various proteolytic and lipolytic enzymes. The data from the growth kinetics indicated that the maximum bacteriocin production occurred in the late log phase. Overall, our results signify the potential of pyocin SA189 as a bio-control agent.
authors.affiliation: Department of Microbiology, Federal Urdu University of Arts, Science and Technology, Karachi, Pakistan.
authors.forename: Sehar Afshan
authors.initials: SA
authors.lastname: Naz
authors.affiliation: Department of Microbiology, Federal Urdu University of Arts, Science and Technology, Karachi, Pakistan.
authors.forename: Nusrat
authors.initials: N
authors.lastname: Jabeen
authors.affiliation: University of Karachi, Karachi, Pakistan.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Sohail
authors.affiliation: University of Karachi, Karachi, Pakistan.
authors.forename: Sheikh Ajaz
authors.initials: SA
authors.lastname: Rasool
mesh_terms: D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D058507:Host Specificity; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000378:metabolism*; D011709:Pyocins / Q000737:chemistry / Q000302:isolation & purification*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013696:Temperature
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D011709:Pyocins; D012336:RNA, Ribosomal, 16S