title: Alveolar macrophage phenotype expression in airway-instilled bone marrow cells in mice.
abstract: No uniform consensus has been established regarding post-pneumonectomy lung regeneration. This study was undertaken to determine whether airway-instilled lung- or bone marrow-derived cells are able to differentiate and reconstitute as lung component cells in the course of post-pneumonectomy lung growth. Bone marrow cells or lung cells obtained from C57 black (BL)/6-GFP mice were intratracheally instilled into C57BL/6 mice treated with left pneumonectomy and cell differentiation was examined. It is unclear whether intratracheally instilled lung or bone marrow cells differentiate into non-hematopoietic cells after pneumonectomy. However, regardless of whether pneumonectomy is performed, intratracheally instilled bone marrow cells display a surface antigen profile that is similar to alveolar macrophages. Furthermore, these newly differentiated macrophages function similarly to resident macrophages in terms of TNF-α production, suggesting that bone marrow stem cells acquire the same macrophage phenotype. In conclusion, intratracheally instilled bone marrow cells adapt to the surrounding microenvironment, directly differentiating into alveolar macrophages, and remain in the alveolar space for at least 3 months.
authors.affiliation: Division of General Thoracic Surgery, Department of Surgery, School of Medicine, Keio University, Shinjuku-ku, Tokyo, 160-8582 Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Okui
authors.affiliation: Division of General Thoracic Surgery, Department of Surgery, School of Medicine, Keio University, Shinjuku-ku, Tokyo, 160-8582 Japan.
authors.forename: Taichiro
authors.initials: T
authors.lastname: Goto
authors.affiliation: Division of General Thoracic Surgery, Department of Surgery, School of Medicine, Keio University, Shinjuku-ku, Tokyo, 160-8582 Japan.
authors.forename: Keisuke
authors.initials: K
authors.lastname: Asakura
authors.affiliation: Division of General Thoracic Surgery, Department of Surgery, School of Medicine, Keio University, Shinjuku-ku, Tokyo, 160-8582 Japan.
authors.forename: Ikuo
authors.initials: I
authors.lastname: Kamiyama
authors.affiliation: Division of General Thoracic Surgery, Department of Surgery, School of Medicine, Keio University, Shinjuku-ku, Tokyo, 160-8582 Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Ohtsuka
keywords: Alveolar macrophage; Bone marrow; Differentiation; Hematopoietic stem cells; Pneumonectomy