title: Extraction and free radical scavenging activity of polysaccharide from 'Anji Baicha' (Camellia sinensis (L.) O. Kuntze).
abstract: In this study, the optimization of the extraction conditions of polysaccharide from 'Anji Baicha' (Camellia sinensis (L.) O. Kuntze) (AP) was investigated by response surface methodology (RSM). Three main independent variables (extraction temperature, time, ratio of water to raw material) were taken into consideration. And then the free radical scavenging activities of the sample were investigated including scavenging effects of superoxide and hydroxyl radicals. The RSM analysis showed good correspondence between experimental and predicted values.. The optimal condition to obtain the highest yield of AP was determined as follows: temperature 76.79 °C, time 2.48 h, ratio of water to material 22.53 mL/g. For the free radical scavenging activity, the IC50 values of Vc and AP were 7.78 and 83.25 μg/mL. And for the scavenging effect on hydroxyl radical, that of AP and Vc were 1.80 and 1.69 mg/mL. AP showed excellent antioxidant activity. This exhibited AP had a good potential for antioxidant. The purification and structure needs to be study in further.
authors.affiliation: Department of Medicine, Huzhou University, Huzhou 313000, China. Electronic address: zhzs@hutc.zj.cn.
authors.forename: Zhongshan
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Medicine, Huzhou University, Huzhou 313000, China.
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Medicine, Huzhou University, Huzhou 313000, China.
authors.forename: Jingfen
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Medicine, Huzhou University, Huzhou 313000, China.
authors.forename: Guozhi
authors.initials: G
authors.lastname: Wang
authors.affiliation: Zhejiang Provincial Key Lab of Geriatrics & Geriatrics Research Institute of Zhejiang Province, Zhejiang Hospital, Hangzhou 310013, China. Electronic address: maogenxiang@163.com.
authors.forename: Genxiang
authors.initials: G
authors.lastname: Mao
mesh_terms: D028241:Camellia sinensis / Q000737:chemistry*; D005591:Chemical Fractionation; D016166:Free Radical Scavengers / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D008962:Models, Theoretical; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology*; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D016166:Free Radical Scavengers; D010936:Plant Extracts; D011134:Polysaccharides
keywords: Camellia sinensis (L.) O. Kuntze; Polysaccharide; Response surface methodology