title: Effects of Nitrogen and Phosphorus on Biochemical Composition of Microalgae Polyculture Harvested from Phycoremediation of Piggery Wastewater Digestate.
abstract: The effects of nitrogen (N) and/or phosphorus (P) starvation on the biochemical composition of native microalgae Chlorella spp. polyculture obtained from the phycoremediation of swine wastewaters were investigated. Microalgae-specific growth rate of 1.2 day(-1) was achieved (30.3 mg L(-1) day(-1)). PO4 (-2) and NH3 were completely removed from swine digestate effluent after 3 and 11 days, respectively. Microalgae harvested immediately after nutrient removal showed high protein (56-59 %) and carbohydrate (25-34 %) but low lipid (1.8-3 %) contents. Depletion of N or P alone stimulated carbohydrate production at the expenses of proteins. Significant lipid accumulation from 3 % ± 0.5 to 16.3 % ± 0.8 was reached only after 25 days following N and P starvation as demonstrated by Nile red-stained cells. Regarding to the effects of harvesting methods on cellular biochemical composition, circumstantial evidences indicate that coagulation-flocculation with tannin may lead to lower protein and lipid amounts but increased carbohydrate content (p < 0.01) as compared to centrifugation.
authors.affiliation: Department of Chemical Engineering, Federal University of Santa Catarina, Florianópolis, SC, 88040-900, Brazil.
authors.forename: William
authors.initials: W
authors.lastname: Michelon
authors.affiliation: EMBRAPA Swine and Poultry, P.O. Box 321, Concórdia, SC, 89700-000, Brazil. marcio.busi@embrapa.br.
authors.forename: Marcio Luis Busi
authors.initials: ML
authors.lastname: Da Silva
authors.affiliation: Biotechnology and Sciences Program, West University of Santa Catarina, Videira, SC, 89560-000, Brazil.
authors.forename: Melissa Paola
authors.initials: MP
authors.lastname: Mezzari
authors.affiliation: Department of Chemical Engineering, Federal University of Santa Catarina, Florianópolis, SC, 88040-900, Brazil.
authors.forename: Mateus
authors.initials: M
authors.lastname: Pirolli
authors.affiliation: Department of Chemical Engineering, Federal University of Santa Catarina, Florianópolis, SC, 88040-900, Brazil.
authors.forename: Jean Michel
authors.initials: JM
authors.lastname: Prandini
authors.affiliation: Department of Chemical Engineering, Federal University of Santa Catarina, Florianópolis, SC, 88040-900, Brazil.
authors.forename: Hugo Moreira
authors.initials: HM
authors.lastname: Soares
mesh_terms: D000818:Animals; D001673:Biodegradation, Environmental; D002241:Carbohydrates / Q000096:biosynthesis* / Q000737:chemistry; D002708:Chlorella / Q000378:metabolism; D008055:Lipids / Q000096:biosynthesis / Q000737:chemistry; D058086:Microalgae / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism*; D010758:Phosphorus / Q000378:metabolism*; D013552:Swine; D013634:Tannins / Q000737:chemistry / Q000378:metabolism; D062065:Wastewater / Q000737:chemistry
chemical_list: D002241:Carbohydrates; D008055:Lipids; D009584:Nitrogen; D010758:Phosphorus; D013634:Tannins; D062065:Wastewater
keywords: Carbohydrate; Lipid; Microalgae; Phycoremediation; Protein; Swine wastewater