title: Kinetics and microbial ecology of batch sulfidogenic bioreactors for co-treatment of municipal wastewater and acid mine drainage.
abstract: The kinetics and microbial ecology in sulfidogenic bioreactors used in a novel two-stage process for co-treatment of acid mine drainage (AMD) and municipal wastewater (MWW) were investigated. Michaelis-Menten modeling of COD oxidation by sulfate reducing bacteria (SRB) (Vmax=0.33mgL(-1)min(-1), Km=4.3mgL(-1)) suggested that the Vmax can be reasonably achieved given the typical COD values in MWW and anticipated mixing with AMD. Non-competitive inhibition modeling (Ki=6.55mgL(-1)) indicated that excessive iron level should be avoided to limit its effects on SRB. The COD oxidation rate was positively correlated to COD/sulfate ratio and SRB population, as evidenced by dsrA gene copies. Phylogenetic analysis revealed diverse microbial communities dominated by sulfate reducing delta-proteobacteria. Microbial community and relative quantities of SRB showed significant differences under different COD/sulfate ratios (0.2, 1 and 2), and the highest dsrA gene concentration and most complex microbial diversity were observed under COD/sulfate ratio 2. Major species were associated with Desulfovirga, Desulfobulbus, Desulfovibrio, and Syntrophus sp. The reported COD kinetics, SRB abundances and the phylogenetic profile provide insights into the co-treatment process and help identify the parameters of concerns for such technology development.
authors.affiliation: Department of Civil and Environmental Engineering, West Virginia University, Morgantown, WV 26506-6103, United States.
authors.forename: Dongyang
authors.initials: D
authors.lastname: Deng
authors.affiliation: Department of Civil and Environmental Engineering, West Virginia University, Morgantown, WV 26506-6103, United States.
authors.forename: Jennifer L
authors.initials: JL
authors.lastname: Weidhaas
authors.affiliation: Department of Civil and Environmental Engineering, West Virginia University, Morgantown, WV 26506-6103, United States. Electronic address: lianshin.lin@mail.wvu.edu.
authors.forename: Lian-Shin
authors.initials: LS
authors.lastname: Lin
mesh_terms: D057919:Biological Oxygen Demand Analysis; D019149:Bioreactors / Q000382:microbiology*; D007220:Industrial Waste; D007700:Kinetics; D008906:Mining; D010084:Oxidation-Reduction; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D016999:Sulfur-Reducing Bacteria / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D014865:Waste Disposal, Fluid / Q000379:methods; D062065:Wastewater
chemical_list: D007220:Industrial Waste; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D062065:Wastewater
keywords: Acid mine drainage (AMD); Kinetics modelling; Municipal wastewater (MWW); Phylogenetic analysis; Sulfidogenic bioreactors