title: Evaluation of Canine Pancreas-Specific Lipase Activity, Lipase Activity, and Trypsin-Like Immunoreactivity in an Experimental Model of Acute Kidney Injury in Dogs.
abstract: BACKGROUND
  Diagnosis of pancreatitis in dogs is complicated by extrapancreatic disorders that can alter the results of laboratory tests. Extrapancreatic disorders can also affect the diagnosis of exocrine pancreatic insufficiency (EPI). The effects of acute kidney injury (AKI) on pancreas-specific lipase activity (Spec cPL(®) Test), serum lipase activity and trypsin-like immunoreactivity (TLI) in dogs have not been evaluated.
  
  
  OBJECTIVE
  Serum Spec cPL, lipase activity, and TLI concentrations will increase secondary to decreased kidney function.
  
  
  METHODS
  Five purpose-bred dogs.
  
  
  METHODS
  Experimental prospective study. Gentamicin was used to induce AKI in 5 purpose-bred dogs. Serum samples were collected for measurement of creatinine, Spec cPL, lipase activity and TLI over 60 days, during both induction of, and recovery from, AKI.
  
  
  RESULTS
  All dogs developed and recovered from AKI. Six of 52 (12%) serum Spec cPL concentrations were increased (2 in the equivocal zone and 4 consistent with pancreatitis) in 2 of 5 (40%) dogs. Two of 51 (4%) serum lipase activity values were increased in 2 of 5 dogs. Serum TLI was increased above the reference range in 17 of 50 (34%) samples in 3 of 5 dogs. For all biomarkers, there was no consistent correlation with increases in serum creatinine concentration.
  
  
  CONCLUSIONS
  Decreased renal excretion during experimental AKI did not cause consistent and correlated increases in serum Spec cPL, lipase activity, or TLI in this cohort of dogs.
authors.affiliation: Veterinary Medical Teaching Hospital, University of California-Davis, Davis, CA.
authors.forename: S E
authors.initials: SE
authors.lastname: Hulsebosch
authors.affiliation: Department of Medicine & Epidemiology, School of Veterinary Medicine, Davis, CA.
authors.forename: C A
authors.initials: CA
authors.lastname: Palm
authors.affiliation: Koret School of Veterinary Medicine, The Hebrew University of Jerusalem, Jerusalem, Israel.
authors.forename: G
authors.initials: G
authors.lastname: Segev
authors.affiliation: Department of Medicine & Epidemiology, School of Veterinary Medicine, Davis, CA.
authors.forename: L D
authors.initials: LD
authors.lastname: Cowgill
authors.affiliation: Department of Population, Health & Reproduction, School of Veterinary Medicine, Davis, CA.
authors.forename: P H
authors.initials: PH
authors.lastname: Kass
authors.affiliation: Department of Medicine & Epidemiology, School of Veterinary Medicine, Davis, CA.
authors.forename: S L
authors.initials: SL
authors.lastname: Marks
mesh_terms: D058186:Acute Kidney Injury / Q000139:chemically induced / Q000378:metabolism / Q000662:veterinary*; D000818:Animals; D015415:Biomarkers; D003404:Creatinine / Q000097:blood; D004283:Dog Diseases / Q000139:chemically induced* / Q000378:metabolism; D004285:Dogs; D005260:Female; D005839:Gentamicins / Q000633:toxicity; D008049:Lipase / Q000097:blood / Q000378:metabolism*; D010179:Pancreas / Q000201:enzymology*; D011500:Protein Synthesis Inhibitors / Q000633:toxicity; D014357:Trypsin / Q000097:blood / Q000276:immunology*
chemical_list: D015415:Biomarkers; D005839:Gentamicins; D011500:Protein Synthesis Inhibitors; D003404:Creatinine; D008049:Lipase; D014357:Trypsin
keywords: Creatinine; Exocrine pancreatic insufficiency; Pancreatitis; Spec cPL