title: Differential Receptor for Advanced Glycation End Products Expression in Preeclamptic, Intrauterine Growth Restricted, and Gestational Diabetic Placentas.
abstract: OBJECTIVE
  Receptor for advanced glycation end products (RAGE) is a receptor implicated in the modulation of inflammation. Inflammation has been associated with pregnancy pathologies including preeclampsia (PE), intrauterine growth restriction (IUGR), and gestational diabetes mellitus (GDM). Our objective was to examine placental RAGE expression in PE, IUGR, and GDM complications.
  
  
  METHODS
  Human placental tissues were obtained for RAGE determination using Q-PCR, immunohistochemistry, and Western blot. Invasive trophoblast cells were cultured and treated with AGES for RAGE activation studies.
  
  
  RESULTS
  Compared to control placenta, we observed: (i) decreased RAGE gene expression during GDM, (ii) increased RAGE protein in the PE placenta, and (iii) decreased RAGE protein in the IUGR placenta. In trophoblast cells exposed AGEs, we observed: (i) decreased trophoblast invasion, (ii) increased c-Jun N-terminal kinases (JNK) and Extracellular signal-regulated kinases (ERK), and (iii) increased TNF-α and IL-1β secretion.
  
  
  CONCLUSIONS
  We conclude that placental RAGE is activated during PE and that RAGE-mediated inflammation in the trophoblast involves increased pro-inflammatory cytokine secretion.
authors.affiliation: Lung and Placenta Research Laboratory, Physiology and Developmental Biology, Brigham Young University, Provo, UT, USA.
authors.forename: Kristen L
authors.initials: KL
authors.lastname: Alexander
authors.affiliation: Lung and Placenta Research Laboratory, Physiology and Developmental Biology, Brigham Young University, Provo, UT, USA.
authors.forename: Camilo A
authors.initials: CA
authors.lastname: Mejia
authors.affiliation: Lung and Placenta Research Laboratory, Physiology and Developmental Biology, Brigham Young University, Provo, UT, USA.
authors.forename: Clinton
authors.initials: C
authors.lastname: Jordan
authors.affiliation: Lung and Placenta Research Laboratory, Physiology and Developmental Biology, Brigham Young University, Provo, UT, USA.
authors.forename: Michael B
authors.initials: MB
authors.lastname: Nelson
authors.affiliation: Lung and Placenta Research Laboratory, Physiology and Developmental Biology, Brigham Young University, Provo, UT, USA.
authors.forename: Brian M
authors.initials: BM
authors.lastname: Howell
authors.affiliation: Lung and Placenta Research Laboratory, Physiology and Developmental Biology, Brigham Young University, Provo, UT, USA.
authors.forename: Cameron M
authors.initials: CM
authors.lastname: Jones
authors.affiliation: Lung and Placenta Research Laboratory, Physiology and Developmental Biology, Brigham Young University, Provo, UT, USA.
authors.forename: Paul R
authors.initials: PR
authors.lastname: Reynolds
authors.affiliation: Lung and Placenta Research Laboratory, Physiology and Developmental Biology, Brigham Young University, Provo, UT, USA.
authors.forename: Juan A
authors.initials: JA
authors.lastname: Arroyo
mesh_terms: D000328:Adult; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D016640:Diabetes, Gestational / Q000378:metabolism*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D005260:Female; D005317:Fetal Growth Retardation / Q000378:metabolism*; D017127:Glycation End Products, Advanced / Q000494:pharmacology; D006801:Humans; D053583:Interleukin-1beta / Q000378:metabolism; D048031:JNK Mitogen-Activated Protein Kinases / Q000378:metabolism; D010920:Placenta / Q000378:metabolism; D011225:Pre-Eclampsia / Q000378:metabolism*; D011247:Pregnancy; D000067759:Receptor for Advanced Glycation End Products / Q000235:genetics / Q000378:metabolism*; D014327:Trophoblasts / Q000378:metabolism / Q000502:physiology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D017127:Glycation End Products, Advanced; D053583:Interleukin-1beta; D000067759:Receptor for Advanced Glycation End Products; D014409:Tumor Necrosis Factor-alpha; D048049:Extracellular Signal-Regulated MAP Kinases; D048031:JNK Mitogen-Activated Protein Kinases
keywords: Diabetes Mellitus; intrauterine growth restriction; placenta; preeclampsia; receptors for advanced glycation end products