title: No Polymorphism in Plasmodium falciparum K13 Propeller Gene in Clinical Isolates from Kolkata, India.
abstract: Molecular markers associated with artemisinin resistance in Plasmodium falciparum are yet to be well defined. Recent studies showed that polymorphisms in K13 gene are associated with artemisinin resistance. The present study was designed to know the pattern of polymorphisms in propeller region of K13 gene among the clinical isolates collected from urban Kolkata after five years of ACT implementation. We collected 59 clinical isolates from urban Kolkata and sequenced propeller region of K13 gene in 51 isolates successfully. We did not find any mutation in any isolate. All patients responded to the ACT, a combination of artesunate + sulphadoxine-pyrimethamine. The drug regimen is still effective in the study area and there is no sign of emergence of resistance against artemisinin as evidenced by wild genotype of K13 gene in all isolates studied.
authors.affiliation: Protozoology Unit, Department of Microbiology, Calcutta School of Tropical Medicine, 108 Chittaranjan Avenue, Kolkata 700 073, India.
authors.forename: Moytrey
authors.initials: M
authors.lastname: Chatterjee
authors.affiliation: Protozoology Unit, Department of Microbiology, Calcutta School of Tropical Medicine, 108 Chittaranjan Avenue, Kolkata 700 073, India ; Department of Microbiology, NRS Medical College, 138 AJC Bose Road, Kolkata 700 014, India.
authors.forename: Swagata
authors.initials: S
authors.lastname: Ganguly
authors.affiliation: Protozoology Unit, Department of Microbiology, Calcutta School of Tropical Medicine, 108 Chittaranjan Avenue, Kolkata 700 073, India ; Department of Zoology, APC Roy Government College, Himachal Bihar, Matigara, Siliguri, West Bengal 734 010, India.
authors.forename: Pabitra
authors.initials: P
authors.lastname: Saha
authors.affiliation: Department of Zoology, Ballygunge Science College, University of Calcutta, 35 Ballygunge Circular Road, Kolkata 700 019, India.
authors.forename: Biswabandhu
authors.initials: B
authors.lastname: Bankura
authors.affiliation: Calcutta School of Tropical Medicine, 108 Chittaranjan Avenue, Kolkata 700 073, India.
authors.forename: Nandita
authors.initials: N
authors.lastname: Basu
authors.affiliation: Department of Zoology, Ballygunge Science College, University of Calcutta, 35 Ballygunge Circular Road, Kolkata 700 019, India.
authors.forename: Madhusudan
authors.initials: M
authors.lastname: Das
authors.affiliation: Department of Tropical Medicine, Calcutta School of Tropical Medicine, 108 Chittaranjan Avenue, Kolkata 700 073, India.
authors.forename: Subhasish K
authors.initials: SK
authors.lastname: Guha
authors.affiliation: Protozoology Unit, Department of Microbiology, Calcutta School of Tropical Medicine, 108 Chittaranjan Avenue, Kolkata 700 073, India.
authors.forename: Ardhendu K
authors.initials: AK
authors.lastname: Maji