title: Chromatographically isolated CD63+CD81+ extracellular vesicles from mesenchymal stromal cells rescue cognitive impairments after TBI.
abstract: Extracellular vesicles (EVs) secreted by cells present an attractive strategy for developing new therapies, but progress in the field is limited by several issues: The quality of the EVs varies with the type and physiological status of the producer cells; protocols used to isolate the EVs are difficult to scale up; and assays for efficacy are difficult to develop. In the present report, we have addressed these issues by using human mesenchymal stem/stromal cells (MSCs) that produce EVs when incubated in a protein-free medium, preselecting the preparations of MSCs with a biomarker for their potency in modulating inflammation, incubating the cells in a chemically defined protein-free medium that provided a stable environment, isolating the EVs with a scalable chromatographic procedure, and developing an in vivo assay for efficacy of the cells in suppressing neuroinflammation after traumatic brain injury (TBI) in mice. In addition, we demonstrate that i.v. infusion of the isolated EVs shortly after induction of TBI rescued pattern separation and spatial learning impairments 1 mo later.
authors.affiliation: Institute for Regenerative Medicine at Scott & White, College of Medicine, Texas A&M Health Science Center, Temple, TX 76502.
authors.forename: Dong-ki
authors.initials: DK
authors.lastname: Kim
authors.affiliation: Institute for Regenerative Medicine at Scott & White, College of Medicine, Texas A&M Health Science Center, Temple, TX 76502.
authors.forename: Hidetaka
authors.initials: H
authors.lastname: Nishida
authors.affiliation: Institute for Regenerative Medicine at Scott & White, College of Medicine, Texas A&M Health Science Center, Temple, TX 76502.
authors.forename: Su Yeon
authors.initials: SY
authors.lastname: An
authors.affiliation: Institute for Regenerative Medicine at Scott & White, College of Medicine, Texas A&M Health Science Center, Temple, TX 76502.
authors.forename: Ashok K
authors.initials: AK
authors.lastname: Shetty
authors.affiliation: Institute for Regenerative Medicine at Scott & White, College of Medicine, Texas A&M Health Science Center, Temple, TX 76502.
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Bartosh
authors.affiliation: Institute for Regenerative Medicine at Scott & White, College of Medicine, Texas A&M Health Science Center, Temple, TX 76502 Prockop@medicine.tamhsc.edu.
authors.forename: Darwin J
authors.initials: DJ
authors.lastname: Prockop
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis; D001930:Brain Injuries / Q000150:complications* / Q000523:psychology; D002478:Cells, Cultured; D002852:Chromatography, Ion Exchange; D003072:Cognition Disorders / Q000209:etiology / Q000523:psychology / Q000628:therapy*; D016895:Culture Media, Serum-Free; D004660:Encephalitis / Q000209:etiology / Q000523:psychology / Q000628:therapy*; D000067128:Extracellular Vesicles / Q000737:chemistry*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000737:chemistry* / Q000648:ultrastructure; D051379:Mice; D065853:Spatial Learning; D060225:Tetraspanin 28 / Q000032:analysis; D060149:Tetraspanin 30 / Q000032:analysis
chemical_list: D015415:Biomarkers; D016895:Culture Media, Serum-Free; D060225:Tetraspanin 28; D060149:Tetraspanin 30
keywords: MSCs; efficacy assay; exosomes; neuroinflammation