title: Differential protein expression in the estuarine copepod Eurytemora affinis after diuron and alkylphenol exposures.
abstract: Proteomics was used in the calanoid copepod Eurytemora affinis for screening of protein expression modifications induced by organic contaminants. The copepods were exposed in a continuous flow-through system for 86 h to environmentally relevant concentrations of contaminants representative of the pollution in the Seine Estuary (Haute-Normandie, France; diuron, 500 ng L(-1) ; alkylphenol mixture, 1000 ng L(-1) ). Proteome analysis of whole-body copepod extracts by 2-dimensional gel electrophoresis revealed that the contaminants induced modifications in protein expression, with the highest quantitative variations occurring after diuron exposure. Specifically, 88 and 41 proteins were differentially expressed after diuron and alkylphenol treatments, respectively. After mass spectrometry analysis, 51 (diuron exposure) and 15 (alkylphenol exposure) proteins were identified. The identified proteins were potentially related to energy metabolism, cell growth, nervous signal conductivity, excitotoxicity, oxidative stress response, and antioxidant defense. The data suggest a massive general disturbance of physiological functions of E. affinis after diuron exposure, whereas alkylphenols induced an alteration of a few targeted physiological functions. The protein expression signatures identified after contaminant exposure deserve further investigation in terms of the development of novel potential biomarkers for water quality assessment. Environ Toxicol Chem 2016;35:1860-1871. © 2015 SETAC.
authors.affiliation: UMR-I 02 Laboratoire Stress Environnementaux et BIOsurveillance des milieux aquatiques (SEBIO), FR CNRS 3730 SCALE, University of Le Havre, Normandy University, Le Havre, France.
authors.forename: Céline
authors.initials: C
authors.lastname: Boulangé-Lecomte
authors.affiliation: UMR-I 02 Laboratoire Stress Environnementaux et BIOsurveillance des milieux aquatiques (SEBIO), FR CNRS 3730 SCALE, University of Le Havre, Normandy University, Le Havre, France.
authors.forename: Béatrice
authors.initials: B
authors.lastname: Rocher
authors.affiliation: UMR-I 02 Laboratoire Stress Environnementaux et BIOsurveillance des milieux aquatiques (SEBIO), FR CNRS 3730 SCALE, University of Le Havre, Normandy University, Le Havre, France.|UMR CNRS 5805, Environnements et Paléoenvironnements Océaniques et Continentaux (EPOC), Laboratoire de Physico- et Toxico-Chimie de l'environnement (LPTC), Bordeaux University, Talence, France.|UMR CNRS 8187 Laboratoire d'Océanologie et de Géosciences (LOG), Wimereux Marine Station, Lille 1 University, Wimereux, France.
authors.forename: Kévin
authors.initials: K
authors.lastname: Cailleaud
authors.affiliation: UMR CNRS 6270, Laboratoire Polymères-Biopolymères-Surfaces (LPBS), University of Rouen, Normandy University, Mont-Saint-Aignan, France.
authors.forename: Pascal
authors.initials: P
authors.lastname: Cosette
authors.affiliation: UMR-I 02 Laboratoire Stress Environnementaux et BIOsurveillance des milieux aquatiques (SEBIO), FR CNRS 3730 SCALE, University of Le Havre, Normandy University, Le Havre, France.
authors.forename: Eléna
authors.initials: E
authors.lastname: Legrand
authors.affiliation: UMR CNRS 8187 Laboratoire d'Océanologie et de Géosciences (LOG), Wimereux Marine Station, Lille 1 University, Wimereux, France.
authors.forename: David
authors.initials: D
authors.lastname: Devreker
authors.affiliation: UMR CNRS 5805, Environnements et Paléoenvironnements Océaniques et Continentaux (EPOC), Laboratoire de Physico- et Toxico-Chimie de l'environnement (LPTC), Bordeaux University, Talence, France.
authors.forename: Hélène
authors.initials: H
authors.lastname: Budzinski
authors.affiliation: UMR CNRS 8187 Laboratoire d'Océanologie et de Géosciences (LOG), Wimereux Marine Station, Lille 1 University, Wimereux, France.
authors.forename: Sami
authors.initials: S
authors.lastname: Souissi
authors.affiliation: UMR-I 02 Laboratoire Stress Environnementaux et BIOsurveillance des milieux aquatiques (SEBIO), FR CNRS 3730 SCALE, University of Le Havre, Normandy University, Le Havre, France.
authors.forename: Joëlle
authors.initials: J
authors.lastname: Forget-Leray
mesh_terms: D000818:Animals; D033342:Copepoda / Q000187:drug effects / Q000378:metabolism*; D004237:Diuron / Q000633:toxicity*; D015180:Electrophoresis, Gel, Two-Dimensional; D004734:Energy Metabolism / Q000187:drug effects; D013058:Mass Spectrometry; D010636:Phenols / Q000737:chemistry / Q000633:toxicity*; D020543:Proteome / Q000032:analysis / Q000187:drug effects*; D014874:Water Pollutants, Chemical / Q000737:chemistry / Q000633:toxicity*
chemical_list: D010636:Phenols; D020543:Proteome; D014874:Water Pollutants, Chemical; D004237:Diuron
keywords: 2D electrophoresis; Biomarkers; Calanoid; Estuary; Organic contaminants