title: Characterization and expression analysis of a caspase-2 in an invertebrate echinoderm sea cumber Apostichopus japonicus.
abstract: Caspase-2 is the most evolutionarily conserved member of the caspase family which mediates the programmed cell death and plays crucial roles in key cellular processes. In this study, a caspase-2 homolog was identified and functionally characterized in sea cucumber Apostichopus japonicus, which we named AjCASP. The full-length cDNA consists of 2100 bp with an ORF encoding a protein of 378 amino acids. The deduced amino acid sequence shows that AjCASP consists of a conserved CARD-CASP2 domain and a CASs domain containing two active residues, two proteolytic cleavage residues, a substrate pocket and a dimer interface as well. In addition, a p20 large subunit with a characteristic five-peptide motif (QACRG) and a p10 small subunit in C-terminal were identified in CASs domain. Above data demonstrated that AjCASP is similar to CED-3 (the caspase-2 homolog of nematode Caenorhabditis elegans), which is further confirmed by phylogenetic tree analysis. AjCASP was ubiquitously expressed in sea cucumber and the obviously higher expression level was observed in coelomocyte, respiratory tree and intestine. Real-time PCR analyses further demonstrated that AjCASP was significantly induced by LPS. Taken together, these results strongly suggest that AjCASP is a caspase-2 homolog and it may be involved in invertebrate immune response, especially in eliminating and degrading invading pathogens.
authors.affiliation: Dalian Ocean University, China.
authors.forename: Shigen
authors.initials: S
authors.lastname: Ye
authors.affiliation: Dalian Ocean University, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Dalian Ocean University, China.
authors.forename: Shengnan
authors.initials: S
authors.lastname: Wang
authors.affiliation: Dalian Ocean University, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Li
authors.affiliation: Dalian Ocean University, China.
authors.forename: Ruijun
authors.initials: R
authors.lastname: Li
authors.affiliation: Dalian Ocean University, China. Electronic address: lihua@dlou.edu.cn.
authors.forename: Hua
authors.initials: H
authors.lastname: Li
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D053143:Caspase 2 / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics; D007413:Intestinal Mucosa / Q000378:metabolism; D008070:Lipopolysaccharides; D008969:Molecular Sequence Data; D012137:Respiratory System / Q000378:metabolism; D047528:Stichopus / Q000235:genetics / Q000276:immunology / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D008070:Lipopolysaccharides; D053143:Caspase 2
keywords: Apostichopus japonicus; Caspase-2; Immune response; LPS challenge