title: Molecular typing of canine Giardia duodenalis isolates from Minas Gerais, Brazil.
abstract: Giardia duodenalis (syn. Giardia intestinalis and Giardia lamblia) is a widespread intestinal parasite in mammals, including humans and pets worldwide. It should be considered a species complex and comprises eight assemblage (A-H). This works aimed to determine the genotypic variability among G. duodenalis isolates from dogs from Minas Gerais state, Brazil. Fecal samples of 97 dogs, from 1-to-10 months old from 15 commercial kennels, were collected and analyzed by the zinc sulfate centrifugal flotation technique, to determine their positivity for G. duodenalis cysts. Cysts pellets were stored and submitted to PCR and nested-PCR reactions with gdh and tpi primers, and then sequencing. Among positive samples (n = 19), fragment amplifications of gdh and tpi genes was observed in 16 (84,2%) and 14 (73,6%), respectively. In total, 30 sequences were obtained. Sequencing analysis showed that for gdh, all isolates were identified as host-specific genotype D, and for tpi, besides host-specific genotype C, were also observed zoonotic genotypes A and B. This study provides, for the first time, current information about genetic characterization of G. duodenalis isolates found in dogs in Minas Gerais state.
authors.affiliation: Federal University of Uberlandia - Biomedical Science Institute Laboratory of Parasitology, Para Avenue, 1720 - Umuarama Campus, 38400-902, Uberlandia, Minas Gerais, Brazil.
authors.forename: Natália M N
authors.initials: NM
authors.lastname: Fava
authors.affiliation: University of Sao Paulo - College of Veterinarian Medicine Medicina, Prof. Dr. Orlando Marques de Paiva Avenue, 87 - Cidade Universitária, Sao Paulo, SP, Brazil.
authors.forename: Rodrigo M
authors.initials: RM
authors.lastname: Soares
authors.affiliation: Federal University of Uberlandia - Biomedical Science Institute Laboratory of Parasitology, Para Avenue, 1720 - Umuarama Campus, 38400-902, Uberlandia, Minas Gerais, Brazil.
authors.forename: Luana A M
authors.initials: LA
authors.lastname: Scalia
authors.affiliation: Federal University of Uberlandia - Biomedical Science Institute Laboratory of Parasitology, Para Avenue, 1720 - Umuarama Campus, 38400-902, Uberlandia, Minas Gerais, Brazil.
authors.forename: Maria Júlia Rodrigues da
authors.initials: MJ
authors.lastname: Cunha
authors.affiliation: Federal University of Uberlandia - Biomedical Science Institute Laboratory of Parasitology, Para Avenue, 1720 - Umuarama Campus, 38400-902, Uberlandia, Minas Gerais, Brazil.
authors.forename: Elaine S M
authors.initials: ES
authors.lastname: Faria
authors.affiliation: Federal University of Uberlandia - Biomedical Science Institute Laboratory of Parasitology, Para Avenue, 1720 - Umuarama Campus, 38400-902, Uberlandia, Minas Gerais, Brazil. Electronic address: cury@umuarama.ufu.br.
authors.forename: Márcia Cristina
authors.initials: MC
authors.lastname: Cury
mesh_terms: D017677:Age Distribution; D000818:Animals; D001938:Brazil / Q000453:epidemiology; D016054:DNA, Protozoan / Q000737:chemistry / Q000302:isolation & purification*; D004283:Dog Diseases / Q000453:epidemiology / Q000469:parasitology*; D004285:Dogs; D005243:Feces / Q000469:parasitology; D005260:Female; D016829:Giardia lamblia / Q000145:classification / Q000235:genetics*; D005873:Giardiasis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D005969:Glutamate Dehydrogenase / Q000235:genetics; D008297:Male; D015995:Prevalence; D016415:Sequence Alignment / Q000662:veterinary; D017422:Sequence Analysis, DNA / Q000662:veterinary; D014305:Triose-Phosphate Isomerase / Q000235:genetics
chemical_list: D016054:DNA, Protozoan; D005969:Glutamate Dehydrogenase; D014305:Triose-Phosphate Isomerase
keywords: Dogs; Genotyping; Giardia duodenalis; Variability