title: A facile and practical biosensor for choline based on manganese dioxide nanoparticles synthesized in-situ at the surface of electrode by one-step electrodeposition.
abstract: In this paper, a facile and sensitive biocompatible biosensor based on Nafion/choline oxidase/manganese dioxide composite film was developed for the determination of choline chloride. Manganese dioxide (MnO2) nanoparticles, possessing the advantages of large specific surface areas, good hydrophilicity, great permeability as well as excellent biocompatibility, were synthesized in-situ at the surface of the glassy carbon electrode (GCE) by one-step electrodeposition. And then, choline oxidase (ChOx) was immobilized on the MnO2 modified GCE with coating a Nafion film to hold the ChOx/MnO2 film on the electrode surface firmly. Upon optimized conditions, a linear range of 8.0-1.0 mM was obtained for the sensor in a cyclic voltammetry method, with a detection limit as low as 5.0 µM. Besides, the biosensor was successfully employed to detect choline in milk, milk powder and feedstuff samples, providing a promising alternative for the practical application.
authors.affiliation: Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection, and State Key Laboratory of Environmental Health (Incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, #13 Hangkong Road, Wuhan, Hubei 430030, China.
authors.forename: Guangxia
authors.initials: G
authors.lastname: Yu
authors.affiliation: Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection, and State Key Laboratory of Environmental Health (Incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, #13 Hangkong Road, Wuhan, Hubei 430030, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection, and State Key Laboratory of Environmental Health (Incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, #13 Hangkong Road, Wuhan, Hubei 430030, China.
authors.forename: Weixiang
authors.initials: W
authors.lastname: Wu
authors.affiliation: Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection, and State Key Laboratory of Environmental Health (Incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, #13 Hangkong Road, Wuhan, Hubei 430030, China.
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Wei
authors.affiliation: Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection, and State Key Laboratory of Environmental Health (Incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, #13 Hangkong Road, Wuhan, Hubei 430030, China. Electronic address: qi_weiliao@126.com.
authors.forename: Qing
authors.initials: Q
authors.lastname: Lu
mesh_terms: D000429:Alcohol Oxidoreductases / Q000737:chemistry; D055162:Biocatalysis; D015374:Biosensing Techniques / Q000379:methods*; D002794:Choline / Q000032:analysis*; D004563:Electrochemistry; D004566:Electrodes; D004595:Electroplating; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D005465:Fluorocarbon Polymers / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D017895:Manganese Compounds / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry; D010087:Oxides / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D004800:Enzymes, Immobilized; D005465:Fluorocarbon Polymers; D017895:Manganese Compounds; D037742:Nanotubes, Carbon; D010087:Oxides; C040402:perfluorosulfonic acid; D006046:Gold; D000429:Alcohol Oxidoreductases; C019888:choline oxidase; D002794:Choline; C016552:manganese dioxide
keywords: Choline biosensors; Electrochemical; Electrodeposition; Manganese dioxide