title: PLGA-Der p1 Vaccine Inhibited Tumor Growth in a Murine Model of Lung Cancer.
abstract: OBJECTIVE
  We undertook this study to investigate the influence of PLGA-Dermatophagoides protein 1 (Der p1) vaccine on the growth of Lewis lung cancer (LLC) cells in C57/BL/6 mice.
  
  
  METHODS
  After subcutaneous transplantation of LLC, cells were injected into the axilla of C57/BL/6 mice. Five mice received intraperitoneal (i.p.) injection of PLGA-Der p1 vaccine, and another five mice received i.p. injection of PBS or PLGA as control. Body weight of C57/BL/6 mice and tumor growth were measured. Morphological change of tumor was observed under optical microscope. The expression of Ki67 and CD34 protein was detected by immunohistochemistry analysis. The concentration of IL-4 and IFN-γ in the splenocyte culture medium was detected by ELISA.
  
  
  RESULTS
  The growth of transplanted tumor was inhibited markedly by PLGA-Der p1 vaccine. The protein level of Ki67 and CD34 was significantly lower in PLGA-Der p1 vaccine group than in the control group (p < 0.05). The level of IFN-γ of the splenocyte culture medium was significantly higher in PLGA-Der p1 vaccine group than that in the control group (p < 0.05). However, the level of IL-4 in the splenocyte culture medium was obviously lower in PLGA-Der p1 vaccine group than that in the control group (p < 0.05).
  
  
  CONCLUSIONS
  PLGA-Der p1 vaccine has a significant inhibitory effect on the growth of LLC cells in C57/BL/6 mice by inducing the production of a Th1 cytokine profile.
authors.affiliation: School of Medicine, University of South China, Hengyang, Hunan, PR China; State Key Laboratory of Respiratory Disease for Allergy at Shengzhen University, School of Medicine, Shenzhen University, Shenzhen, Guangdong, PR China; The Affiliated Nanhua Hospital, University of South China, Hengyang, Hunan, PR China. Electronic address: jhzuoch@sina.com.
authors.forename: Jianhong
authors.initials: J
authors.lastname: Zuo
authors.affiliation: The Affiliated Nanhua Hospital, University of South China, Hengyang, Hunan, PR China.
authors.forename: Meiling
authors.initials: M
authors.lastname: Wen
authors.affiliation: Department of Respiratory and Critical Care Medicine, Zhangjiajie City Hospital, Zhangjiajie, Hunan, China.
authors.forename: Mingsheng
authors.initials: M
authors.lastname: Lei
authors.affiliation: State Key Laboratory of Respiratory Disease for Allergy at Shengzhen University, School of Medicine, Shenzhen University, Shenzhen, Guangdong, PR China.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Xiao
authors.affiliation: State Key Laboratory of Respiratory Disease for Allergy at Shengzhen University, School of Medicine, Shenzhen University, Shenzhen, Guangdong, PR China.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Liu
keywords: Cancer vaccines; Dermatophagoides protein 1; Immunotherapy; Lung cancer