title: A New Approach Using Manganese-Enhanced MRI to Diagnose Acute Mesenteric Ischemia in a Rabbit Model: Initial Experience.
abstract: OBJECTIVE
  Manganese-enhanced MRI (MEMRI) has been applied to a wide range of biological and disease research. The purpose of the study was to use MEMRI to diagnose the acute mesenteric ischemia (AMI).
  
  
  METHODS
  The institutional experimental animal ethics committee approved this study. To optimize the dose of Mn(2+) infusion, a dose-dependent curve was obtained using Mn(2+)-enhanced T 1 map MRI by an intravenous infusion 2.5-20 nmol/g body weight (BW) of 50 nmol/L MnCl2. The eighteen animals were divided into control, sham-operated, and AMI groups. AMI models were performed by ligating the superior mesenteric artery (SMA). T 1 values were measured on T 1 maps in regions of the small intestinal wall and relaxation rate (ΔR 1) was calculated.
  
  
  RESULTS
  A nonlinear relationship between infused MnCl2 solution dose and increase in small intestinal wall ΔR 1 was observed. Control animal exhibited significant Mn(2+) clearance over time at the dose of 15 nmol/g BW. In the AMI model, ΔR 1 values (0.95 ± 0.13) in the small intestinal wall were significantly lower than in control group (2.05 ± 0.19) after Mn(2+) infusion (P < 0.01).
  
  
  CONCLUSIONS
  The data suggest that MEMRI shows potential as a diagnostic technique that is directly sensitive to the poor or absent perfusion in AMI.
authors.affiliation: Department of Radiology, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing 400042, China.
authors.forename: Da-Wei
authors.identifier: 0000-0002-7817-4719
authors.initials: DW
authors.lastname: Zhao
authors.affiliation: Department of Radiology, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing 400042, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Cheng
authors.affiliation: Department of Radiology, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing 400042, China.
authors.forename: Lian-Qin
authors.initials: LQ
authors.lastname: Kuang
authors.affiliation: Department of Radiology, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing 400042, China.
authors.forename: Yu-Long
authors.initials: YL
authors.lastname: Zhang
authors.affiliation: Department of Radiology, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing 400042, China.
authors.forename: Hai-Yun
authors.initials: HY
authors.lastname: Cheng
authors.affiliation: Department of Radiology, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing 400042, China.
authors.forename: Jia-Yan
authors.initials: JY
authors.lastname: Min
authors.affiliation: Department of Radiology, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing 400042, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D002712:Chlorides / Q000008:administration & dosage*; D003287:Contrast Media / Q000008:administration & dosage; D004195:Disease Models, Animal; D006801:Humans; D007421:Intestine, Small / Q000000981:diagnostic imaging* / Q000473:pathology; D008279:Magnetic Resonance Imaging / Q000379:methods*; D017895:Manganese Compounds / Q000008:administration & dosage*; D065666:Mesenteric Ischemia / Q000000981:diagnostic imaging* / Q000473:pathology; D011817:Rabbits; D011859:Radiography
chemical_list: D002712:Chlorides; D003287:Contrast Media; D017895:Manganese Compounds; C025340:manganese chloride