title: Diterpenoids from the Roots of Euphorbia fischeriana with Inhibitory Effects on Nitric Oxide Production.
abstract: Bioactivity-guided isolation of a methanolic extract of Euphorbia fischeriana led to the isolation of four new abietane-type diterpenoids, fischeriolides A-D (1-4), together with 11 known diterpenoids. Their structures were elucidated based on the interpretation of 1D and 2D NMR spectroscopic and HRESIMS data. The absolute configuration of compound 3 was determined by single-crystal X-ray diffraction analysis and electronic circular dichroism methods. Compounds 5-9 exhibited inhibitory effects on LPS-induced nitric oxide production in RAW 264.7 macrophages with IC50 values in the range 4.9-12.6 μM.
authors.affiliation: College of Pharmacy, Chungbuk National University , Cheongju 28644, Korea.
authors.forename: Jin Woo
authors.initials: JW
authors.lastname: Lee
authors.affiliation: College of Pharmacy, Chungbuk National University , Cheongju 28644, Korea.
authors.forename: Chul
authors.initials: C
authors.lastname: Lee
authors.affiliation: College of Pharmacy, Chungbuk National University , Cheongju 28644, Korea.
authors.forename: Qinghao
authors.initials: Q
authors.lastname: Jin
authors.affiliation: College of Pharmacy, Chungbuk National University , Cheongju 28644, Korea.
authors.forename: Hari
authors.initials: H
authors.lastname: Jang
authors.affiliation: Department of Biosystems and Biotechnology, College of Life Sciences and Biotechnology, Korea University , Seoul 02841, Korea.
authors.forename: Dongho
authors.initials: D
authors.lastname: Lee
authors.affiliation: Western Seoul Center, Korea Basic Science Institute , Seoul 03759, Korea.
authors.forename: Ha-Jin
authors.initials: HJ
authors.lastname: Lee
authors.affiliation: Beamline Department, Pohang Accelerator Laboratory/POSTECH , Pohang 37673, Korea.
authors.forename: Jong Won
authors.initials: JW
authors.lastname: Shin
authors.affiliation: College of Pharmacy, Chungbuk National University , Cheongju 28644, Korea.
authors.forename: Sang Bae
authors.initials: SB
authors.lastname: Han
authors.affiliation: College of Pharmacy, Chungbuk National University , Cheongju 28644, Korea.
authors.forename: Jin Tae
authors.initials: JT
authors.lastname: Hong
authors.affiliation: College of Pharmacy, Chungbuk National University , Cheongju 28644, Korea.
authors.forename: Youngsoo
authors.initials: Y
authors.lastname: Kim
authors.affiliation: College of Pharmacy, Chungbuk National University , Cheongju 28644, Korea.
authors.forename: Mi Kyeong
authors.initials: MK
authors.lastname: Lee
authors.affiliation: College of Pharmacy, Chungbuk National University , Cheongju 28644, Korea.
authors.forename: Bang Yeon
authors.initials: BY
authors.lastname: Hwang
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry; D018360:Crystallography, X-Ray; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D028481:Euphorbia / Q000737:chemistry*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects; D051379:Mice; D008968:Molecular Conformation; D015394:Molecular Structure; D009569:Nitric Oxide / Q000096:biosynthesis*; D018517:Plant Roots / Q000737:chemistry; D056910:Republic of Korea
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D004224:Diterpenes; D004365:Drugs, Chinese Herbal; D008070:Lipopolysaccharides; D009569:Nitric Oxide