title: RIG-G inhibits the proliferation of NB4 cells and propels ATRA-induced differentiation of APL cells.
abstract: RIG-G (retinoic acid-induced gene G) was originally identified in ATRA (all-trans retinoic acid)-treated NB4 acute promyelocytic leukemia (APL) cells. It was induced to expression by ATRA along with the differentiation of the cells. However, little is known about its role(s). Here, we established a RIG-G stably expression transformant of NB4 cells. By using the transformant, we showed that expression of RIG-G in NB4 cells not only arrested the cells at G1/G0 transition phase and inhibited their proliferation, but also markedly drive the maturation of NB4 cells in the presence of very low concentration of ATRA (10(-9)mol/L). What's more, by detecting the expression of RIG-G in fresh primary bone marrow mononuclear cells of APL patients in different morbid states, we found high RIG-G expression level in complete remission patients, while low level in untreated or relapsed patients. These results indicated that RIG-G level was high in maturated cells and low in blast cells, and suggested that RIG-G might play a role in the differentiation of bone marrow hemocytes in vivo.
authors.affiliation: State Key Laboratory of Medical Genomics, Faculty of Medical Laboratory Science and Shanghai Institute of Hematology, Rui-jin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, PR China; Department of Hematology, Institute of Hematology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, Zhejiang, PR China.
authors.forename: Ye-jiang
authors.initials: YJ
authors.lastname: Lou
authors.affiliation: State Key Laboratory of Medical Genomics, Faculty of Medical Laboratory Science and Shanghai Institute of Hematology, Rui-jin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, PR China.
authors.forename: Xiao-rong
authors.initials: XR
authors.lastname: Pan
authors.affiliation: State Key Laboratory of Medical Genomics, Faculty of Medical Laboratory Science and Shanghai Institute of Hematology, Rui-jin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, PR China.
authors.forename: Pei-min
authors.initials: PM
authors.lastname: Jia
authors.affiliation: Department of Hematology, Institute of Hematology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, Zhejiang, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Jin
authors.affiliation: State Key Laboratory of Medical Genomics, Faculty of Medical Laboratory Science and Shanghai Institute of Hematology, Rui-jin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, PR China. Electronic address: tongjh@hotmail.com.
authors.forename: Jian-hua
authors.initials: JH
authors.lastname: Tong
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000502:physiology*; D015473:Leukemia, Promyelocytic, Acute / Q000473:pathology*; D012074:Remission Induction; D014212:Tretinoin / Q000494:pharmacology*
chemical_list: C116468:IFIT3 protein, human; D047908:Intracellular Signaling Peptides and Proteins; D014212:Tretinoin
keywords: Acute promyelocytic leukemia; All-trans retinoic acid; Differentiation; Proliferation; RIG-G