title: Bacillus piscicolasp. nov., isolated from Thai fish sauce (Nam-pla).
abstract: A Gram-reaction-positive, spore-forming, rod-shaped bacterium, designated NR1-3-2T, was isolated from fish sauce collected from a factory in Chonburi province, Thailand. Strain NR1-3-2Tgrew at pH 5-10 (optimum 7.5), at 21-48 °C (optimum 37 °C) and with 0-15 % (w/v) NaCl (optimum 1-3 %). The diamino acid found in the cell-wall peptidoglycan wasmeso-diaminopimelic acid. Menaquinone with seven isoprene units (MK-7) was the major isoprenoid quinone. The strain contained anteiso-C15 : 0, iso-C15 : 0and anteiso-C17 : 0as major cellular fatty acids. Diphosphatidylglycerol, phosphatidyl glycerol and one unknown glycolipid were detected as major polar lipids. On the basis of 16S rRNA gene sequence analysis, strain NR1-3-2Tbelonged to the genusBacillusand was closely related toBacillus iranensisDSM 23995T(97.4 % similarity). Strain NR1-3-2Texhibited low DNA-DNA relatedness (31.2-39.8 %) withB. iranensisDSM 23995T. The DNA G+C content was 44.2 mol%. On the basis of phenotypic characteristics, DNA-DNA relatedness and phylogenetic analyses, the strain is considered to represent a novel species of the genusBacillusand the nameBacillus piscicolasp. nov. is proposed. The type strain is NR1-3-2T( = JCM 19598T= LMG 28281T= PCU 340T= TISTR 2295T).
authors.affiliation: Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand.
authors.forename: Rungsima
authors.initials: R
authors.lastname: Daroonpunt
authors.affiliation: Japan Collection of Microorganisms, RIKEN BioResource Center, Tsukuba, Ibaraki 305-0074, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Itoh
authors.affiliation: Japan Collection of Microorganisms, RIKEN BioResource Center, Tsukuba, Ibaraki 305-0074, Japan.
authors.forename: Takuji
authors.initials: T
authors.lastname: Kudo
authors.affiliation: Japan Collection of Microorganisms, RIKEN BioResource Center, Tsukuba, Ibaraki 305-0074, Japan.
authors.forename: Moriya
authors.initials: M
authors.lastname: Ohkuma
authors.affiliation: Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand.
authors.forename: Somboon
authors.initials: S
authors.lastname: Tanasupawat