title: New Commercially Available IgG Kits and Time-Resolved Fluorometric IgE Assay for Diagnosis of Allergic Bronchopulmonary Aspergillosis in Patients with Cystic Fibrosis.
abstract: Allergic bronchopulmonary aspergillosis (ABPA) is difficult to diagnose; diagnosis relies on clinical, radiological, pathological, and serological criteria. Our aim was to assess the performance of two new commercially available kits and a new in-house assay: an Aspergillus fumigatus enzyme-linked immunosorbent assay (ELISA) IgG kit (Bordier Affinity Products), an Aspergillus Western blotting IgG kit (LDBio Diagnostics), and a new in-house time-resolved fluorometric IgE assay (dissociation-enhanced lanthanide fluorescent immunoassay, or DELFIA) using recombinant proteins from an Aspergillus sp. recently developed by our laboratory for ABPA diagnosis in a retrospective study that included 26 cystic fibrosis patients. Aspergillus fumigatus-specific IgG levels measured by a commercial ELISA kit were in accordance with the level of precipitins currently used in our lab. The ELISA kit could accelerate and help standardize ABPA diagnosis. Aspergillus fumigatus-specific IgE levels measured by ImmunoCAP (Phadia) with A. fumigatus M3 antigen and by DELFIA with a purified protein extract of A. fumigatus were significantly correlated (P < 10(-6)). The results with recombinant antigens glucose-6-phosphate isomerase and mannitol-1-phosphate dehydrogenase were encouraging but must be confirmed with sera from more patients. The DELFIA is an effective tool that can detect specific IgE against more fungal allergens than can be detected with other commercially available tests.
authors.affiliation: UMR6249 Chrono-environnement, University of Franche-Comté, Besançon, France Parasitology-Mycology Department, University Hospital of Besançon, Besançon, France.
authors.forename: Coralie
authors.initials: C
authors.lastname: Barrera
authors.affiliation: Pneumology Department, University Hospital of Besançon, Besançon, France.
authors.forename: Bénédicte
authors.initials: B
authors.lastname: Richaud-Thiriez
authors.affiliation: UMR6249 Chrono-environnement, University of Franche-Comté, Besançon, France Parasitology-Mycology Department, University Hospital of Besançon, Besançon, France.
authors.forename: Steffi
authors.initials: S
authors.lastname: Rocchi
authors.affiliation: UMR6249 Chrono-environnement, University of Franche-Comté, Besançon, France Parasitology-Mycology Department, University Hospital of Besançon, Besançon, France.
authors.forename: Bénédicte
authors.initials: B
authors.lastname: Rognon
authors.affiliation: UMR6249 Chrono-environnement, University of Franche-Comté, Besançon, France Parasitology-Mycology Department, University Hospital of Besançon, Besançon, France.
authors.forename: Sandrine
authors.initials: S
authors.lastname: Roussel
authors.affiliation: UMR6249 Chrono-environnement, University of Franche-Comté, Besançon, France Parasitology-Mycology Department, University Hospital of Besançon, Besançon, France.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Grenouillet
authors.affiliation: UMR6249 Chrono-environnement, University of Franche-Comté, Besançon, France Parasitology-Mycology Department, University Hospital of Besançon, Besançon, France.
authors.forename: Audrey
authors.initials: A
authors.lastname: Laboissière
authors.affiliation: UMR6249 Chrono-environnement, University of Franche-Comté, Besançon, France Pneumology Department, University Hospital of Besançon, Besançon, France.
authors.forename: Jean-Charles
authors.initials: JC
authors.lastname: Dalphin
authors.affiliation: UMR6249 Chrono-environnement, University of Franche-Comté, Besançon, France Parasitology-Mycology Department, University Hospital of Besançon, Besançon, France.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Reboux
authors.affiliation: UMR6249 Chrono-environnement, University of Franche-Comté, Besançon, France Parasitology-Mycology Department, University Hospital of Besançon, Besançon, France laurence.millon@univ-fcomte.fr.
authors.forename: Laurence
authors.initials: L
authors.lastname: Millon
mesh_terms: D000293:Adolescent; D000328:Adult; D000485:Allergens / Q000032:analysis / Q000276:immunology; D000908:Antibodies, Fungal / Q000097:blood*; D000946:Antigens, Fungal / Q000032:analysis / Q000276:immunology; D001229:Aspergillosis, Allergic Bronchopulmonary / Q000175:diagnosis* / Q000276:immunology; D001232:Aspergillus fumigatus / Q000276:immunology; D015153:Blotting, Western / Q000379:methods*; D003550:Cystic Fibrosis / Q000150:complications; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D005956:Glucose-6-Phosphate Isomerase / Q000097:blood; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood* / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D011933:Reagent Kits, Diagnostic; D013401:Sugar Alcohol Dehydrogenases / Q000097:blood; D055815:Young Adult
chemical_list: D000485:Allergens; D000908:Antibodies, Fungal; D000946:Antigens, Fungal; D007074:Immunoglobulin G; D011933:Reagent Kits, Diagnostic; D007073:Immunoglobulin E; D013401:Sugar Alcohol Dehydrogenases; C021544:mannitol-1-phosphate dehydrogenase; D005956:Glucose-6-Phosphate Isomerase