title: MicroRNA-128 inhibits EMT of human osteosarcoma cells by directly targeting integrin α2.
abstract: Deregulated expression of miRNAs contributes to the development of osteosarcoma. The present study was to evaluate the level of miR-128 and integrin α2 (ITGA2) in osteosarcoma tissues and cells. We further investigated the molecular mechanisms of miR-128 and ITGA2 in osteosarcoma cell lines. In the present study, we found that miR-128 expression was down-regulated in osteosarcoma tissues and MG-63, U2OS, and SAOS-2 cells (all p < 0.001). By contrast, ITGA2 was up-regulated. Furthermore, we found that miR-128 overexpression suppressed cell migration and invasion of MG-63 cells. Mechanically, miR-128 overexpression inhibited epithelial-mesenchymal transition (EMT) of MG-63 cells. Importantly, we identified that the 3'-untranslated region (3'-UTR) of ITGA2 was a direct target of miR-128. Luciferase reporter assays confirmed that miR-128 binding to the 3'-UTR regions of ITGA2 inhibited the expression of ITGA2 in MG-63 cells. At the same time, overexpressed ITGA2 also reversed EMT inhibited by miR-128. In conclusion, this study suggested that high miR-128 expression suppressed osteosarcoma cell migration, invasion, and EMT development through targeting ITGA2, which may be recommended as a therapeutic target for osteosarcoma.
authors.affiliation: Department of Orthopaedics, Yidu Central Hospital of Weifang, Qingzhou, 262500, Shandong, People's Republic of China.
authors.forename: Xinsheng
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Orthopaedics, Yidu Central Hospital of Weifang, Qingzhou, 262500, Shandong, People's Republic of China.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Liang
authors.affiliation: Department of Orthopaedics, Yidu Central Hospital of Weifang, Qingzhou, 262500, Shandong, People's Republic of China.
authors.forename: Kehai
authors.initials: K
authors.lastname: Gao
authors.affiliation: Department of Orthopaedics, Yidu Central Hospital of Weifang, Qingzhou, 262500, Shandong, People's Republic of China.
authors.forename: Huazhuang
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Orthopaedics, Yidu Central Hospital of Weifang, Qingzhou, 262500, Shandong, People's Republic of China.
authors.forename: Guangzong
authors.initials: G
authors.lastname: Zhao
authors.affiliation: Department of Orthopaedics, Yidu Central Hospital of Weifang, Qingzhou, 262500, Shandong, People's Republic of China.
authors.forename: Shantao
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Orthopaedics, Yidu Central Hospital of Weifang, Qingzhou, 262500, Shandong, People's Republic of China. fangjunweifang@163.com.
authors.forename: Jun
authors.initials: J
authors.lastname: Fang
mesh_terms: D015153:Blotting, Western; D001859:Bone Neoplasms / Q000235:genetics* / Q000378:metabolism; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics*; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D039421:Integrin alpha2 / Q000235:genetics / Q000378:metabolism*; D035683:MicroRNAs / Q000378:metabolism*; D009361:Neoplasm Invasiveness / Q000235:genetics; D012516:Osteosarcoma / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection; D014945:Wound Healing
chemical_list: D039421:Integrin alpha2; C545189:MIRN128 microRNA, human; D035683:MicroRNAs
keywords: EMT; ITGA2; Osteosarcoma; miR-128