title: Using non-invasive molecular spectroscopic techniques to detect unique aspects of protein Amide functional groups and chemical properties of modeled forage from different sourced-origins.
abstract: The non-invasive molecular spectroscopic technique-FT/IR is capable to detect the molecular structure spectral features that are associated with biological, nutritional and biodegradation functions. However, to date, few researches have been conducted to use these non-invasive molecular spectroscopic techniques to study forage internal protein structures associated with biodegradation and biological functions. The objectives of this study were to detect unique aspects and association of protein Amide functional groups in terms of protein Amide I and II spectral profiles and chemical properties in the alfalfa forage (Medicago sativa L.) from different sourced-origins. In this study, alfalfa hay with two different origins was used as modeled forage for molecular structure and chemical property study. In each forage origin, five to seven sources were analyzed. The molecular spectral profiles were determined using FT/IR non-invasive molecular spectroscopy. The parameters of protein spectral profiles included functional groups of Amide I, Amide II and Amide I to II ratio. The results show that the modeled forage Amide I and Amide II were centered at 1653 cm(-1) and 1545 cm(-1), respectively. The Amide I spectral height and area intensities were from 0.02 to 0.03 and 2.67 to 3.36 AI, respectively. The Amide II spectral height and area intensities were from 0.01 to 0.02 and 0.71 to 0.93 AI, respectively. The Amide I to II spectral peak height and area ratios were from 1.86 to 1.88 and 3.68 to 3.79, respectively. Our results show that the non-invasive molecular spectroscopic techniques are capable to detect forage internal protein structure features which are associated with forage chemical properties.
authors.affiliation: Tianjin Agricultural University, 22 Jinjing Road, Tianjin 300384, China; Department of Animal and Poultry Science, College of Agriculture and Bioresources, University of Saskatchewan, 51 Campus Drive, Saskatoon, SK, Canada, S7N 5A8.
authors.forename: Cuiying
authors.initials: C
authors.lastname: Ji
authors.affiliation: Tianjin Agricultural University, 22 Jinjing Road, Tianjin 300384, China. Electronic address: zhangxuewei63@163.com.
authors.forename: Xuewei
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Tianjin Agricultural University, 22 Jinjing Road, Tianjin 300384, China; Department of Animal and Poultry Science, College of Agriculture and Bioresources, University of Saskatchewan, 51 Campus Drive, Saskatoon, SK, Canada, S7N 5A8. Electronic address: peiqiang.yu@usask.ca.
authors.forename: Peiqiang
authors.initials: P
authors.lastname: Yu
mesh_terms: D000577:Amides / Q000737:chemistry*; D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D004044:Dietary Proteins / Q000737:chemistry*; D000455:Medicago sativa / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry*; D017433:Protein Structure, Secondary; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000577:Amides; D004044:Dietary Proteins; D010940:Plant Proteins
keywords: Chemical properties; Modeled forage; Molecular spectroscopic techniques; Protein functional groups