title: Lampropedia cohaerenssp. nov., a biofilm-forming bacterium isolated from microbial mats of a hot water spring, and emended description of the genusLampropedia.
abstract: A biofilm-forming, Gram-stain-negative, aerobic, catalase-positive but oxidase-negative strain, designated CT6T, was isolated from the microbial mats (∼45 °C) of a hot water spring, located within the Himalayan ranges at Manikaran, Himachal Pradesh, India. Strain CT6Tformed white, smooth colonies with irregular margins. Transmission electron microscopy revealed coccoid, non-flagellated cells with wavy boundaries. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain CT6Tbelongs to the genusLampropediawith a sequence similarity value of 95.4 % to the sole member of this genus,Lampropedia hyalinaATCC 11041T. Strain CT6Twas found to have phosphatidylethanolamine and phosphatidylglycerol as the major polar lipids. The major cellular fatty acids were C16 : 0, summed feature 8 (C18 : 1ω7cand/or C18 : 1ω6c), C14 : 0, C19 : 0ω8ccyclo and summed feature 3 (C16 : 1ω7cand/or C16 : 1ω6c). The major respiratory quinone was ubiquinone-8. The major polyamines were putrescine, spermidine and the betaproteobacterial-specific 2-hydroxyputrescine. The DNA G+C content was 63.5 mol%. Based on the genotypic, phenotypic, physiological and biochemical data, strain CT6Tis considered to represent a novel species of the genusLampropedia, for which the nameLampropedia cohaerenssp. nov. is proposed ( = DSM 100029T= KCTC 42939T= MCC 2711T).
authors.affiliation: Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi- 110007, India.
authors.forename: C
authors.initials: C
authors.lastname: Tripathi
authors.affiliation: Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi- 110007, India.
authors.forename: N K
authors.initials: NK
authors.lastname: Mahato
authors.affiliation: Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi- 110007, India.
authors.forename: A K
authors.initials: AK
authors.lastname: Singh
authors.affiliation: Ciliate Biology Laboratory, SGTB Khalsa College, University of Delhi, Delhi- 110007, India.
authors.forename: K
authors.initials: K
authors.lastname: Kamra
authors.affiliation: Microbial Type Culture Collection & Genebank, Institute of Microbial Technology, Chandigarh- 160036, India.
authors.forename: S
authors.initials: S
authors.lastname: Korpole
authors.affiliation: Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi- 110007, India.
authors.forename: R
authors.initials: R
authors.lastname: Lal