title: Droplet digital polymerase chain reaction for DNMT3A and IDH1/2 mutations to improve early detection of acute myeloid leukemia relapse after allogeneic hematopoietic stem cell transplantation.
authors.affiliation: Unit of Immunogenetics, Leukemia Genomics and Immunobiology, IRCCS San Raffaele Scientific Institute, Milano, Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: Brambati
authors.affiliation: Genomics for the Diagnosis of Human Pathologies Unit, IRCCS San Raffaele Scientific Institute, Milano, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Galbiati
authors.affiliation: Unit of Immunogenetics, Leukemia Genomics and Immunobiology, IRCCS San Raffaele Scientific Institute, Milano, Italy. Unit of Hematology and Bone Marrow Transplantation, IRCCS San Raffaele Scientific Institute, Milano, Italy.
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Xue
authors.affiliation: Unit of Immunogenetics, Leukemia Genomics and Immunobiology, IRCCS San Raffaele Scientific Institute, Milano, Italy.
authors.forename: Cristina
authors.initials: C
authors.lastname: Toffalori
authors.affiliation: Unit of Hematology and Bone Marrow Transplantation, IRCCS San Raffaele Scientific Institute, Milano, Italy.
authors.forename: Lara
authors.initials: L
authors.lastname: Crucitti
authors.affiliation: Unit of Hematology and Bone Marrow Transplantation, IRCCS San Raffaele Scientific Institute, Milano, Italy.
authors.forename: Raffaella
authors.initials: R
authors.lastname: Greco
authors.affiliation: Unit of Hematology and Bone Marrow Transplantation, IRCCS San Raffaele Scientific Institute, Milano, Italy.
authors.forename: Elisa
authors.initials: E
authors.lastname: Sala
authors.affiliation: Molecular Hematology Laboratory, IRCCS San Raffaele Scientific Institute, Milano, Italy.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Crippa
authors.affiliation: Unit of Immunogenetics, Leukemia Genomics and Immunobiology, IRCCS San Raffaele Scientific Institute, Milano, Italy.
authors.forename: Lorenza
authors.initials: L
authors.lastname: Chiesa
authors.affiliation: Genomics for the Diagnosis of Human Pathologies Unit, IRCCS San Raffaele Scientific Institute, Milano, Italy.
authors.forename: Nadia
authors.initials: N
authors.lastname: Soriani
authors.affiliation: Unit of Immunogenetics, Leukemia Genomics and Immunobiology, IRCCS San Raffaele Scientific Institute, Milano, Italy.
authors.forename: Benedetta
authors.initials: B
authors.lastname: Mazzi
authors.affiliation: Molecular Hematology Laboratory, IRCCS San Raffaele Scientific Institute, Milano, Italy.
authors.forename: Cristina
authors.initials: C
authors.lastname: Tresoldi
authors.affiliation: Unit of Hematology and Bone Marrow Transplantation, IRCCS San Raffaele Scientific Institute, Milano, Italy.
authors.forename: Maria Teresa Lupo
authors.initials: MT
authors.lastname: Stanghellini
authors.affiliation: Unit of Hematology and Bone Marrow Transplantation, IRCCS San Raffaele Scientific Institute, Milano, Italy.
authors.forename: Jacopo
authors.initials: J
authors.lastname: Peccatori
authors.affiliation: Unit of Hematology and Bone Marrow Transplantation, IRCCS San Raffaele Scientific Institute, Milano, Italy.
authors.forename: Matteo G
authors.initials: MG
authors.lastname: Carrabba
authors.affiliation: Unit of Hematology and Bone Marrow Transplantation, IRCCS San Raffaele Scientific Institute, Milano, Italy.
authors.forename: Massimo
authors.initials: M
authors.lastname: Bernardi
authors.affiliation: Genomics for the Diagnosis of Human Pathologies Unit, IRCCS San Raffaele Scientific Institute, Milano, Italy.
authors.forename: Maurizio
authors.initials: M
authors.lastname: Ferrari
authors.affiliation: Genomics for the Diagnosis of Human Pathologies Unit, IRCCS San Raffaele Scientific Institute, Milano, Italy.
authors.forename: Vito
authors.initials: V
authors.lastname: Lampasona
authors.affiliation: Unit of Hematology and Bone Marrow Transplantation, IRCCS San Raffaele Scientific Institute, Milano, Italy. ciceri.fabio@hsr.it.
authors.forename: Fabio
authors.initials: F
authors.lastname: Ciceri
authors.affiliation: Unit of Immunogenetics, Leukemia Genomics and Immunobiology, IRCCS San Raffaele Scientific Institute, Milano, Italy. Unit of Hematology and Bone Marrow Transplantation, IRCCS San Raffaele Scientific Institute, Milano, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Vago
mesh_terms: D000328:Adult; D000368:Aged; D000483:Alleles; D014408:Biomarkers, Tumor / Q000235:genetics* / Q000378:metabolism; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000235:genetics* / Q000378:metabolism; D000090205:DNA Methyltransferase 3A; D042241:Early Diagnosis; D005260:Female; D015870:Gene Expression; D005787:Gene Frequency; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D007521:Isocitrate Dehydrogenase / Q000235:genetics* / Q000378:metabolism; D015470:Leukemia, Myeloid, Acute / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology / Q000628:therapy; D008137:Longitudinal Studies; D008297:Male; D008875:Middle Aged; D009154:Mutation; D018365:Neoplasm, Residual; D016133:Polymerase Chain Reaction / Q000295:instrumentation / Q000379:methods*; D012008:Recurrence; D014184:Transplantation, Homologous
chemical_list: D014408:Biomarkers, Tumor; C000717967:DNMT3A protein, human; C538784:IDH2 protein, human; D007521:Isocitrate Dehydrogenase; C543588:IDH1 protein, human; D004248:DNA (Cytosine-5-)-Methyltransferases; D000090205:DNA Methyltransferase 3A
keywords: acute myeloid leukemia; cytogenetics and molecular genetics; droplet digital PCR; minimal residual disease; stem cell transplantation