title: [Effect of Homeobox A13 transfection on epithelial-mesenchymal transition and bone morphogenetic protein-7 expression in kidney tubular epithelial cells].
abstract: OBJECTIVE
  To examine the transfection of Homeobox A13 (HOXA13) on epithelial-mesenchymal transition (EMT) and the expression of bone morphogenetic protein-7 (BMP-7) induced by albumin-overload in human kidney tubular epithelial cells (HKCs).
  
  
  METHODS
  The cultured HKCs were treated with 20 mg/mL human serum albumin (HSA) for 48 hours. Protein expression of cytokeratin (CK), vimentin and HOXA13 in the HKCs was assessed by Western blot. Protein expression of CK, vimentin, and BMP-7 was also detected in HKCs transfected with lipofectamine contained HOXA13 DNA.
  
  
  RESULTS
  HSA induced EMT in HKCs, presented by decreased CK expression (P<0.01) and increased vimentin expression (P<0.01). The up-regulated expression of HOXA13 transfected by lipofectamine inhibited the level of EMT induced by HSA in HKCs (P<0.05). The decreased rate of BMP-7 protein expression induced by HSA was inhibited by over-expressed HOXA13 in HKCs (P<0.05).
  
  
  CONCLUSIONS
  Transfection of HOXA13 in HKCs could inhibit the degree of EMT induced by albumin-overload, possibly by increasing BMP-7 expression.
authors.affiliation: Department of Pediatric Nephrology, Chidren's Medical Center, Second Xiangya Hospital, Central South University, Changsha 410011, China. heqn2629@163.com.
authors.forename: Li
authors.initials: L
authors.lastname: Peng
authors.forename: Qing-Nan
authors.initials: QN
authors.lastname: He
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Li
authors.forename: Lan-Jun
authors.initials: LJ
authors.lastname: Shuai
authors.forename: Hai-Xia
authors.initials: HX
authors.lastname: Chen
authors.forename: Yong-Zhen
authors.initials: YZ
authors.lastname: Li
authors.forename: Zhu-Wen
authors.initials: ZW
authors.lastname: Yi
mesh_terms: D055419:Bone Morphogenetic Protein 7 / Q000235:genetics*; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000378:metabolism; D058750:Epithelial-Mesenchymal Transition; D018398:Homeodomain Proteins / Q000502:physiology*; D006801:Humans; D007633:Keratins / Q000235:genetics; D007684:Kidney Tubules / Q000378:metabolism*; D014162:Transfection; D014746:Vimentin / Q000235:genetics
chemical_list: C525819:BMP7 protein, human; D055419:Bone Morphogenetic Protein 7; D018398:Homeodomain Proteins; D014746:Vimentin; C099959:homeobox protein HOXA13; D007633:Keratins