title: Constructive remodeling of a synthetic endothelial extracellular matrix.
abstract: The construction of well-controllable in vitro models of physiological and pathological vascular endothelium remains a fundamental challenge in tissue engineering and drug development. Here, we present an approach for forming a synthetic endothelial extracellular matrix (ECM) that closely resembles that of the native structure by locally depositing basement membrane materials onto type 1 collagen nanofibers only in a region adjacent to the endothelial cell (EC) monolayer. Culturing the EC monolayer on this synthetic endothelial ECM remarkably enhanced its physiological properties, reducing its vascular permeability, and promoting a stabilized, quiescent phenotype. We demonstrated that the EC monolayer on the synthetic endothelial ECM neither creates non-physiological barriers to cell-cell or cell-ECM interactions, nor hinders molecular diffusion of growth factors and other molecules. The synthetic endothelial ECM and vascular endothelium on it may help us enter in a new phase of research in which various models of the biological barrier behavior can be tested experimentally.
authors.affiliation: The California Institute for Quantitative Biosciences, Stanley Hall, University of California, Berkeley, Berkeley, CA 94720, USA.
authors.forename: Sewoon
authors.initials: S
authors.lastname: Han
authors.affiliation: Department of Mechanical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Ave., CambridgeMA 02139, USA.
authors.forename: Yoojin
authors.initials: Y
authors.lastname: Shin
authors.affiliation: School of Mechanical Engineering, Korea University, 145 Anam-ro, Seongbuk-gu, Seoul, 02841, South Korea.
authors.forename: Hyo Eun
authors.initials: HE
authors.lastname: Jeong
authors.affiliation: Department of Mechanical Engineering, KAIST, 291 Daehak-ro, Yuseong-gu Daejeon 305-701, South Korea.
authors.forename: Jessie S
authors.initials: JS
authors.lastname: Jeon
authors.affiliation: Department of Mechanical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Ave., CambridgeMA 02139, USA.|Department of Biological Engineering, Massachusetts Institute of Technology, 77 Massachusetts Ave., Cambridge MA 02139, USA.
authors.forename: Roger D
authors.initials: RD
authors.lastname: Kamm
authors.affiliation: Department of Bioengineering, University of Pennsylvania, 240 Skirkanich Hall, 210 South 33rd Street Philadelphia PA 19104, USA.
authors.forename: Dongeun
authors.initials: D
authors.lastname: Huh
authors.affiliation: The California Institute for Quantitative Biosciences, Stanley Hall, University of California, Berkeley, Berkeley, CA 94720, USA.|Department of Mechanical Engineering, Etcheverry Hall, University of California, Berkeley, Berkeley, CA 94720, USA.
authors.forename: Lydia L
authors.initials: LL
authors.lastname: Sohn
authors.affiliation: School of Mechanical Engineering, Korea University, 145 Anam-ro, Seongbuk-gu, Seoul, 02841, South Korea.
authors.forename: Seok
authors.initials: S
authors.lastname: Chung
mesh_terms: D001485:Basement Membrane / Q000166:cytology / Q000254:growth & development; D002448:Cell Adhesion / Q000502:physiology; D018929:Cell Culture Techniques / Q000379:methods*; D024042:Collagen Type I / Q000737:chemistry / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology / Q000254:growth & development*; D005109:Extracellular Matrix / Q000502:physiology*; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D044085:Microfluidics / Q000379:methods; D057139:Nanofibers / Q000737:chemistry; D010539:Permeability; D023822:Tissue Engineering
chemical_list: D024042:Collagen Type I; D036341:Intercellular Signaling Peptides and Proteins