title: Molecular Mechanisms of Lipoic Acid Protection against Aflatoxin B₁-Induced Liver Oxidative Damage and Inflammatory Responses in Broilers.
abstract: Alpha-lipoic acid (α-LA) was evaluated in this study for its molecular mechanisms against liver oxidative damage and inflammatory responses induced by aflatoxin B₁ (AFB₁). Birds were randomly allocated into four groups with different diets for three weeks: a basal diet, a 300 mg/kg α-LA supplementation in a basal diet, a diet containing 74 μg/kg AFB₁, and 300 mg/kg α-LA supplementation in a diet containing 74 μg/kg AFB₁. In the AFB₁ group, the expression of GSH-PX mRNA was down-regulated (p < 0.05), and the levels of lipid peroxide and nitric oxide were increased (p < 0.05) in the chicken livers compared to those of the control group. Additionally, the mRNA level of the pro-inflammatory factor interleukin-6 was up-regulated significantly (p < 0.05), the protein expressions of both the nuclear factor kappa B (NF-κB) p65 and the inducible nitric oxide synthase were enhanced significantly (p < 0.05) in the AFB₁ group. All of these negative effects were inhibited by α-LA. These results indicate that α-LA may be effective in preventing hepatic oxidative stress, down-regulating the expression of hepatic pro-inflammatory cytokines, as well as inhibiting NF-κB expression.
authors.affiliation: State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China. maqiugang@cau.edu.cn.
authors.forename: Qiugang
authors.initials: Q
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China. liyan-602@163.com.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China. fanyucau@163.com.
authors.forename: Yu
authors.initials: Y
authors.lastname: Fan
authors.affiliation: State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China. lihongzhao100@126.com.
authors.forename: Lihong
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Translational Medicine Lab, Chinese National Human Genome Center, Beijing 100176, China. huaweimda@126.com.
authors.forename: Hua
authors.initials: H
authors.lastname: Wei
authors.affiliation: State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China. jicheng@cau.edu.cn.
authors.forename: Cheng
authors.initials: C
authors.lastname: Ji
authors.affiliation: State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China. jyzhang@cau.edu.cn.
authors.forename: Jianyun
authors.initials: J
authors.lastname: Zhang
mesh_terms: D016604:Aflatoxin B1 / Q000633:toxicity*; D000818:Animals; D002645:Chickens; D005979:Glutathione Peroxidase / Q000235:genetics; D005982:Glutathione Transferase / Q000235:genetics; D006419:Heme Oxygenase (Decyclizing) / Q000235:genetics; D007249:Inflammation / Q000378:metabolism; D015850:Interleukin-6 / Q000235:genetics; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D020011:Protective Agents / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D013482:Superoxide Dismutase / Q000235:genetics; D008063:Thioctic Acid / Q000494:pharmacology*; D051996:Transcription Factor RelA / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
chemical_list: D015850:Interleukin-6; D020011:Protective Agents; D012333:RNA, Messenger; D051996:Transcription Factor RelA; D014409:Tumor Necrosis Factor-alpha; D008063:Thioctic Acid; D016604:Aflatoxin B1; D005979:Glutathione Peroxidase; D052247:Nitric Oxide Synthase Type II; D006419:Heme Oxygenase (Decyclizing); D013482:Superoxide Dismutase; D005982:Glutathione Transferase
keywords: NF-κB; aflatoxin B1; inflammation; lipoic acid; liver; oxidative stress