title: Antimicrobial and cell viability measurement of bovine serum albumin capped silver nanoparticles (Ag/BSA) loaded collagen immobilized poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) film.
abstract: Bacterial infection of orthopedic devices has been a major concern in joint replacement procedures. Therefore, this study is aimed at formulating collagen immobilized poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) film loaded with bovine serum albumin capped silver nanoparticles (Ag/BSA NPs) to inhibit bacterial growth while retaining/promoting osteoblast cells viability. The nanoparticles loaded collagen immobilized PHBV film was characterized for its composition by X-ray Photoelectron Spectroscopy and Anodic Stripping Voltammetry. The extent of loading of Ag/BSA NPs on collagen immobilized PHBV film was found to depend on the chemistry of the functionalized PHBV film and the concentration of Ag/BSA NPs solution used for loading nanoparticles. Our results showed that more Ag/BSA NPs were loaded on higher molecular weight collagen immobilized PHEMA-g-PHBV film. Maximum loading of Ag/BSA NPs on collagen immobilized PHBV film was observed when 16ppm solution was used for adsorption studies. Colony forming unit and optical density measurements showed broad antimicrobial activity towards Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa at significantly lower concentration i.e., 0.19 and 0.31μg/disc, compared to gentamicin and sulfamethoxazole trimethoprim while MTT assay showed that released nanoparticles from Ag/BSA NPs loaded collagen immobilized PHBV film has no impact on MCTC3-E1 cells viability.
authors.affiliation: Department of Chemistry, Howard University, Washington DC 20059, United States.
authors.forename: Rotimi
authors.initials: R
authors.lastname: Bakare
authors.affiliation: Department of Chemistry, Howard University, Washington DC 20059, United States.
authors.forename: Samantha
authors.initials: S
authors.lastname: Hawthrone
authors.affiliation: Department of Biology, Howard University, Washington DC 20059, United States.
authors.forename: Carmen
authors.initials: C
authors.lastname: Vails
authors.affiliation: Department of Biology, Howard University, Washington DC 20059, United States.
authors.forename: Ayele
authors.initials: A
authors.lastname: Gugssa
authors.affiliation: Department of Polymer Engineering, University of Akron, Akron, OH 44325, United States.
authors.forename: Alamgir
authors.initials: A
authors.lastname: Karim
authors.affiliation: Department of Microbiology, College of Medicine, Howard University, Washington DC 20059, United States.
authors.forename: John
authors.initials: J
authors.lastname: Stubbs
authors.affiliation: Department of Chemistry, Howard University, Washington DC 20059, United States. Electronic address: draghavan@howard.edu.
authors.forename: Dharmaraj
authors.initials: D
authors.lastname: Raghavan
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry*; D001419:Bacteria / Q000254:growth & development*; D002417:Cattle; D002460:Cell Line; D002470:Cell Survival; D003094:Collagen / Q000737:chemistry*; D008567:Membranes, Artificial; D053768:Metal Nanoparticles / Q000737:chemistry*; D051379:Mice; D010006:Osteoblasts / Q000378:metabolism*; D011091:Polyesters / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D012834:Silver / Q000737:chemistry*; D000094122:Polyhydroxybutyrates
chemical_list: D000890:Anti-Infective Agents; D003094:Collagen; D008567:Membranes, Artificial; D011091:Polyesters; D012710:Serum Albumin, Bovine; D012834:Silver; C052620:poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate); D000094122:Polyhydroxybutyrates
keywords: Antimicrobial activity; Atomic absorption spectrometry; Bone cell viability; Collagen immobilized PHBV; Protein conjugated silver nanoparticles