title: Nitrogen deprivation-induced de novo transcriptomic profiling of the oleaginous green alga Botryococcus braunii 779.
abstract: To assess the effect of nitrogen deprivation (ND), a moderately growing A-race Botryococcus braunii subisolate 779 was subjected to nitrogen deprivation for 3 days. De novo transcriptome was assembled and annotated by using Trinity software and Basic Local Alignment Search Tools (BLAST), respectively. Comparative analysis indicates that transcriptomes of A-races differ from those of B-races. Furthermore, majority of the homologous ESTs in A-race but not B-race transcriptomes were unknown sequences. Upon ND, level of photosynthetic transcripts, but not photosynthetic efficiency was downregulated. Unlike hydrocarbon contents, ESTs involved in hydrocarbon biosynthesis were not upregulated. Taken together, our results imply that A- and B-races belong to different B. braunii subspecies. Upon ND, excess photosynthetic transcripts are recycled for nitrogen; and hydrocarbon accumulation is not via de novo biosynthesis. Here we describe in details the data contents and analytic methodologies associated with the data uploaded to Gene Expression Omnibus (accession number GSE71296).
authors.affiliation: Ocean College, Zhejiang University, Hangzhou, Zhejiang 310058, China.
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Xu
authors.affiliation: Ocean Research Centre of Zhoushan, Zhoushan, Zhejiang 316021, China.
authors.forename: Jing
authors.initials: J
authors.lastname: He
authors.affiliation: Ocean College, Zhejiang University, Hangzhou, Zhejiang 310058, China.
authors.forename: Shuyuan
authors.initials: S
authors.lastname: Qi
authors.affiliation: Ocean College, Zhejiang University, Hangzhou, Zhejiang 310058, China ; Ocean Research Centre of Zhoushan, Zhoushan, Zhejiang 316021, China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Liu
keywords: Botryococcus braunii; Hydrocarbons; Illumina paired-end sequencing; Nitrogen deprivation; Transcriptomic profiling