title: Multiprotein Printing by Light-Induced Molecular Adsorption.
abstract: Light-induced molecular adsorption of proteins (LIMAP) allows for quantitative sub-micrometer-resolution printing of multiple biomolecules. Surface-bound gradients are patterned within minutes over an entire glass cover-slip. LIMAP is used to perform selective immuno-assays, to dynamically control the adhesion of individual cells, and to achieve hierarchical co-cultures instrumental for tissue engineering.
authors.affiliation: Interdisciplinary Institute for Neuroscience, University of Bordeaux, F-33077, Bordeaux, France.|CNRS UMR 5297, F-33077, Bordeaux, France.
authors.forename: Pierre-Olivier
authors.initials: PO
authors.lastname: Strale
authors.affiliation: Interdisciplinary Institute for Neuroscience, University of Bordeaux, F-33077, Bordeaux, France.|CNRS UMR 5297, F-33077, Bordeaux, France.|Ecole Nationale Supérieure de Biotechnologie, Université Ali Mendjeli, BP E66, 25100, Constantine, Algeria.
authors.forename: Ammar
authors.initials: A
authors.lastname: Azioune
authors.affiliation: Interdisciplinary Institute for Neuroscience, University of Bordeaux, F-33077, Bordeaux, France.|CNRS UMR 5297, F-33077, Bordeaux, France.
authors.forename: Ghislain
authors.initials: G
authors.lastname: Bugnicourt
authors.affiliation: Interdisciplinary Institute for Neuroscience, University of Bordeaux, F-33077, Bordeaux, France.|CNRS UMR 5297, F-33077, Bordeaux, France.
authors.forename: Yohan
authors.initials: Y
authors.lastname: Lecomte
authors.affiliation: Interdisciplinary Institute for Neuroscience, University of Bordeaux, F-33077, Bordeaux, France.|CNRS UMR 5297, F-33077, Bordeaux, France.
authors.forename: Makhlad
authors.initials: M
authors.lastname: Chahid
authors.affiliation: Interdisciplinary Institute for Neuroscience, University of Bordeaux, F-33077, Bordeaux, France.|CNRS UMR 5297, F-33077, Bordeaux, France.
authors.forename: Vincent
authors.initials: V
authors.lastname: Studer
mesh_terms: D000327:Adsorption; D000818:Animals; D002448:Cell Adhesion; D002460:Cell Line; D005353:Fibronectins / Q000737:chemistry / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000737:chemistry / Q000378:metabolism; D008027:Light; D051379:Mice; D018625:Microscopy, Atomic Force; D008856:Microscopy, Fluorescence; D054802:Molecular Imprinting; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism; D013499:Surface Properties; D023822:Tissue Engineering
chemical_list: D005353:Fibronectins; D011506:Proteins; D049452:Green Fluorescent Proteins
keywords: cell co-culture; photopatterning; poly(ethylene glycol); protein micropatterning; single molecules