title: Introduction of the exogenous NADH coenzyme regeneration system and its influence on intracellular metabolic flux of Paenibacillus polymyxa.
abstract: The NAD(+)-dependent formate dehydrogenase (FDH) gene from Candida boidinii was introduced into Paenibacillus polymyxa ZJ-9. The effects of this exogenous gene on the growth of the recombinant strain P. polymyxa XG-1, FDH activity, intracellular NADH and NAD(+) level and the synthesis of R,R-2,3-butanediol (R,R-2,3-BD) were determined. Results from the fermentation in the 7.5L bioreactor showed that the exogenous FDH was highly expressed in the recombinant strain. The titers of NADH, lactic acid, ethanol, NADH/NAD(+), and CO2 excretion rate (CER) of the recombinant strain increased considerably, while acetoin and formic acid decreased significantly. The highest titers of R,R-2,3-BD by the recombinant strain in batch and fed-batch fermentation were 36.8g/L and 51.3g/L, increased 10.2% and 8.0% compared with the parent strain, respectively. This study confirmed that coenzyme regeneration system can manipulate substance metabolism in bacteria, and is an efficient way for promoting the synthesis of NADH-dependent products.
authors.affiliation: School of Marine and Bioengineering, Yancheng Institute of Technology, Yancheng 224051, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.affiliation: School of Marine and Bioengineering, Yancheng Institute of Technology, Yancheng 224051, PR China; State Key Laboratory of Materials-Oriented Chemical Engineering, College of Food Science and Light Industry, Nanjing University of Technology, Nanjing 211816, PR China.
authors.forename: Youyong
authors.initials: Y
authors.lastname: Xu
authors.affiliation: School of Marine and Bioengineering, Yancheng Institute of Technology, Yancheng 224051, PR China. Electronic address: gaojian@ycit.edu.cn.
authors.forename: Jian
authors.initials: J
authors.lastname: Gao
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Food Science and Light Industry, Nanjing University of Technology, Nanjing 211816, PR China.
authors.forename: Hong
authors.initials: H
authors.lastname: Xu
authors.affiliation: School of Marine and Bioengineering, Yancheng Institute of Technology, Yancheng 224051, PR China; State Key Laboratory of Materials-Oriented Chemical Engineering, College of Food Science and Light Industry, Nanjing University of Technology, Nanjing 211816, PR China.
authors.forename: Can
authors.initials: C
authors.lastname: Cao
authors.affiliation: School of Marine and Bioengineering, Yancheng Institute of Technology, Yancheng 224051, PR China.
authors.forename: Feng
authors.initials: F
authors.lastname: Xue
authors.affiliation: School of Marine and Bioengineering, Yancheng Institute of Technology, Yancheng 224051, PR China.
authors.forename: Ge
authors.initials: G
authors.lastname: Ding
authors.affiliation: School of Marine and Bioengineering, Yancheng Institute of Technology, Yancheng 224051, PR China.
authors.forename: Yingyun
authors.initials: Y
authors.lastname: Peng
mesh_terms: D061249:Batch Cell Culture Techniques; D053898:Biosynthetic Pathways; D002072:Butylene Glycols / Q000378:metabolism; D003067:Coenzymes / Q000378:metabolism*; D005285:Fermentation; D005560:Formate Dehydrogenases / Q000235:genetics / Q000378:metabolism; D005798:Genes, Bacterial; D005822:Genetic Vectors / Q000378:metabolism; D042541:Intracellular Space / Q000378:metabolism*; D064688:Metabolic Flux Analysis; D009243:NAD / Q000378:metabolism*; D056507:Paenibacillus / Q000201:enzymology / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*
chemical_list: D002072:Butylene Glycols; D003067:Coenzymes; D009243:NAD; C026978:2,3-butylene glycol; D005560:Formate Dehydrogenases
keywords: Formate dehydrogenase; Metabolic flux; NADH regeneration; Paenibacillus polymyxa; R,R-2,3-butanediol