title: Effects of L-carnitine against H2O2-induced oxidative stress in grass carp ovary cells (Ctenopharyngodon idellus).
abstract: This study was designed in vitro to investigate the effects of L-carnitine against H2O2-induced oxidative stress in a grass carp (Ctenopharyngodon idellus) ovary cell line (GCO). GCO cells were pre-treated with different concentrations of L-carnitine, followed by incubation with 2.5 mM H2O2 for 1 h to induce oxidative damage. The results indicated that adding L-carnitine at concentrations of 0.01-1 mM into the medium for 12 h significantly increased cell viability. Pre-treatment with L-carnitine at concentrations of 0.1-5 mM for 12 h significantly inhibited 2.5 mM H2O2-induced cell viability loss. The significant decreases in the level of reactive oxygen species and cell apoptosis were observed in 0.5 mM L-carnitine group compared to the H2O2 group. Malondialdehyde values of all of the L-carnitine groups were significantly lower than those of the H2O2 group, while total glutathione levels of all of the L-carnitine groups were significantly higher than of the H2O2 group. The activity of antioxidant enzymes, such as total superoxide dismutase (0.1 and 0.5 mM L-carnitine), catalase (0.5 mM L-carnitine) and γ-glutamyl cysteine synthetase (0.5 and 1 mM L-carnitine), was significantly increased. In addition, pre-treatment of L-carnitine in GCO cells exposed to 2.5 mM H2O2 significantly increased the mRNA expression of copper, zinc superoxide dismutase, catalase (0.5 mM L-carnitine), glutamate cysteine ligase catalytic subunit (0.1-1 mM) and glutathione peroxidase (0.1 mM L-carnitine). In conclusion, L-carnitine promotes GCO cell growth and improves antioxidant function, it plays a protective role against oxidative stress induced by H2O2 in GCO cells, and the appropriate supplemental amount of L-carnitine is 0.1-1 mM.
authors.affiliation: College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China.
authors.forename: Qiuju
authors.initials: Q
authors.lastname: Wang
authors.affiliation: College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Ju
authors.affiliation: College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China.
authors.forename: Yuke
authors.initials: Y
authors.lastname: Chen
authors.affiliation: College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China.
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Dong
authors.affiliation: College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China.
authors.forename: Sha
authors.initials: S
authors.lastname: Luo
authors.affiliation: Fishery Technical Extension Station of Jilin Province, Changchun, 130012, China.
authors.forename: Hongjian
authors.initials: H
authors.lastname: Liu
authors.affiliation: College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China. wangqiuju0439@163.com.
authors.forename: Dongming
authors.initials: D
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002331:Carnitine / Q000494:pharmacology*; D002347:Carps; D002374:Catalase / Q000235:genetics; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D005260:Female; D005721:Glutamate-Cysteine Ligase / Q000235:genetics; D005979:Glutathione Peroxidase / Q000235:genetics; D006861:Hydrogen Peroxide; D010053:Ovary / Q000166:cytology; D018384:Oxidative Stress / Q000187:drug effects*; D012333:RNA, Messenger / Q000378:metabolism; D013482:Superoxide Dismutase / Q000235:genetics
chemical_list: D000975:Antioxidants; D012333:RNA, Messenger; D006861:Hydrogen Peroxide; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005721:Glutamate-Cysteine Ligase; D002331:Carnitine
keywords: Antioxidant compounds; Antioxidant enzymes; Fish; Lipid peroxidation