title: Kainate receptor pore-forming and auxiliary subunits regulate channel block by a novel mechanism.
abstract: CONCLUSIONS
  Kainate receptor heteromerization and auxiliary subunits, Neto1 and Neto2, attenuate polyamine ion-channel block by facilitating blocker permeation. Relief of polyamine block in GluK2/GluK5 heteromers results from a key proline residue that produces architectural changes in the channel pore α-helical region. Auxiliary subunits exert an additive effect to heteromerization, and thus relief of polyamine block is due to a different mechanism. Our findings have broad implications for work on polyamine block of other cation-selective ion channels.
  Channel block and permeation by cytoplasmic polyamines is a common feature of many cation-selective ion channels. Although the channel block mechanism has been studied extensively, polyamine permeation has been considered less significant as it occurs at extreme positive membrane potentials. Here, we show that kainate receptor (KAR) heteromerization and association with auxiliary proteins, Neto1 and Neto2, attenuate polyamine block by enhancing blocker permeation. Consequently, polyamine permeation and unblock occur at more negative and physiologically relevant membrane potentials. In GluK2/GluK5 heteromers, enhanced permeation is due to a single proline residue in GluK5 that alters the dynamics of the α-helical region of the selectivity filter. The effect of auxiliary proteins is additive, and therefore the structural basis of polyamine permeation and unblock is through a different mechanism. As native receptors are thought to assemble as heteromers in complex with auxiliary proteins, our data identify an unappreciated impact of polyamine permeation in shaping the signalling properties of neuronal KARs and point to a structural mechanism that may be shared amongst other cation-selective ion channels.
authors.affiliation: Integrated Program in Neurosciences, McGill University, Montréal, Québec, Canada, H3G 0B1.|Department of Pharmacology and Therapeutics, McGill University, Montréal, Québec, Canada, H3G 0B1.
authors.forename: Patricia M G E
authors.initials: PM
authors.lastname: Brown
authors.affiliation: Graduate Program in Pharmacology, McGill University, Montréal, Québec, Canada, H3G 0B1.|Department of Pharmacology and Therapeutics, McGill University, Montréal, Québec, Canada, H3G 0B1.
authors.forename: Mark R P
authors.initials: MR
authors.lastname: Aurousseau
authors.affiliation: Department of Biochemistry, University of Oxford, Oxford, OX1 3QU, UK.
authors.forename: Maria
authors.initials: M
authors.lastname: Musgaard
authors.affiliation: Department of Biochemistry, University of Oxford, Oxford, OX1 3QU, UK.
authors.forename: Philip C
authors.initials: PC
authors.lastname: Biggin
authors.affiliation: Department of Pharmacology and Therapeutics, McGill University, Montréal, Québec, Canada, H3G 0B1.
authors.forename: Derek
authors.initials: D
authors.lastname: Bowie
mesh_terms: D000818:Animals; D057809:HEK293 Cells; D006801:Humans; D015640:Ion Channel Gating; D026502:LDL-Receptor Related Proteins; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008564:Membrane Potentials; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D011073:Polyamines / Q000378:metabolism*; D000072417:Protein Domains; D051381:Rats; D018092:Kainic Acid Receptors / Q000378:metabolism*; D016194:Receptors, N-Methyl-D-Aspartate; D000097806:GluK2 Kainate Receptor
chemical_list: D026502:LDL-Receptor Related Proteins; D008077:Lipoproteins, LDL; D008565:Membrane Proteins; D011073:Polyamines; D018092:Kainic Acid Receptors; D016194:Receptors, N-Methyl-D-Aspartate; D000097806:GluK2 Kainate Receptor; C539204:NETO2 protein, mouse; C486748:Neto1 protein, mouse