title: Calcinated gold nanoparticle arrays for on-chip, multiplexed and matrix-free mass spectrometric analysis of peptides and small molecules.
abstract: A patterned gold nanoparticle microarray, functionalized with a nanoscale silicate coating, has been developed for on-chip, high-throughput mass spectrometric analyses of biomolecules with minimal sample preparation and reagent costs. Fabrication was realized by the combination of layer-by-layer functionalization of the nanoparticles with suitable polyelectrolytes, followed by fluidic patterning of the glass microarray support and calcination for permanent fixation of the nano-coating. Performance of the microarray was evaluated for surface-assisted laser-desorption/ionization mass spectrometry (SALDI-MS), where the nano-silicate coating was found to enhance SALDI efficiency, resulting in comparable performance to some common organic matrices for small and medium sized molecules. Performance contributing factors of this material have been discussed; heat confinement and interband transition/plasmonic resonance may play important roles. Taking the accessibility of fabrication, performance, and reusability of this substrate together, the material developed here provides a new tool for multiplexed and chip-based mass spectrometric analysis.
authors.affiliation: Environmental Toxicology, University of California, Riverside, California 92521, USA. quan.cheng@ucr.edu.
authors.forename: Samuel S
authors.initials: SS
authors.lastname: Hinman
authors.affiliation: Department of Chemistry, University of California, Riverside, California 92521, USA.
authors.forename: Chih-Yuan
authors.initials: CY
authors.lastname: Chen
authors.affiliation: Department of Chemistry, University of California, Riverside, California 92521, USA.
authors.forename: Jicheng
authors.initials: J
authors.lastname: Duan
authors.affiliation: Environmental Toxicology, University of California, Riverside, California 92521, USA. quan.cheng@ucr.edu and Department of Chemistry, University of California, Riverside, California 92521, USA.
authors.forename: Quan
authors.initials: Q
authors.lastname: Cheng
mesh_terms: D006046:Gold / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods*; D053768:Metal Nanoparticles / Q000737:chemistry*; D010455:Peptides / Q000032:analysis*; D040081:Protein Array Analysis / Q000379:methods*
chemical_list: D010455:Peptides; D006046:Gold