title: Effect of colostrum and milk on small intestine expression of AQP4 and AQP5 in newborn buffalo calves.
abstract: Functional studies indicate differences in newborn gastrointestinal morphology and physiology after a meal. Both water and solutes transfer across the intestinal epithelial membrane appear to occur via aquaporins (AQPs). Given that the physiological roles of AQP4 and AQP5 in the developing intestine have not been fully established, the objective of this investigation was to determine their distribution, expression and respective mRNA in the small intestine of colostrums-suckling buffalo calves by using immunohistochemistry, Western blot, and reverse transcriptase-PCR analysis. Results showed different tissue distribution between AQP4 and AQP5 with the presence of the former along the enteric neurons and the latter in the endocrine cells. Moreover, their expression levels were high in the ileum of colostrum-suckling buffalo calves. The data present a link between feeding, intestinal development and water homeostasis, suggesting the involvement of these channel proteins in intestinal permeability and fluid secretion/absorption during this stage of development after birth.
authors.affiliation: Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, Via Veterinaria 1, Naples 80137, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Squillacioti
authors.affiliation: Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, Via Veterinaria 1, Naples 80137, Italy.
authors.forename: A
authors.initials: A
authors.lastname: De Luca
authors.affiliation: Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, Via Veterinaria 1, Naples 80137, Italy.
authors.forename: M E
authors.initials: ME
authors.lastname: Pero
authors.affiliation: Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, Via Veterinaria 1, Naples 80137, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Vassalotti
authors.affiliation: Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, Via Veterinaria 1, Naples 80137, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Lombardi
authors.affiliation: Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, Via Veterinaria 1, Naples 80137, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Avallone
authors.affiliation: Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, Via Veterinaria 1, Naples 80137, Italy.
authors.forename: N
authors.initials: N
authors.lastname: Mirabella
authors.affiliation: Department of Advanced Biomedical Sciences, University of Naples Federico II, Via Pansini 5, Naples 80131, Italy; Institute of Biostructures and Bioimages, National Research Council, Via De Amicis 95, Naples 80131, Italy. Electronic address: alpelaga@unina.it.
authors.forename: A
authors.initials: A
authors.lastname: Pelagalli
mesh_terms: D000818:Animals; D000831:Animals, Newborn / Q000235:genetics* / Q000378:metabolism; D051401:Aquaporin 4 / Q000235:genetics* / Q000378:metabolism; D051402:Aquaporin 5 / Q000235:genetics* / Q000378:metabolism; D015153:Blotting, Western / Q000662:veterinary; D002020:Buffaloes / Q000235:genetics* / Q000378:metabolism; D003126:Colostrum / Q000378:metabolism*; D015870:Gene Expression; D007150:Immunohistochemistry / Q000662:veterinary; D007421:Intestine, Small / Q000378:metabolism; D008297:Male; D008892:Milk / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary
chemical_list: D051401:Aquaporin 4; D051402:Aquaporin 5
keywords: Aquaporins; Buffalo; Colostrum; Small intestine