title: Complete genome of Streptomyces hygroscopicus subsp. limoneus KCTC 1717 (=KCCM 11405), a soil bacterium producing validamycin and diverse secondary metabolites.
abstract: Streptomyces hygroscopicus subsp. limoneus is a Gram-positive, aerobic, aerial mycelial, spore-forming bacterium that was first isolated from a soil sample in Akashi City, Hyogo Prefecture, Japan. We here report the complete genome of S. hygroscopicus subsp. limoneus KCTC 1717 (=KCCM 11405=IFO 12704=ATCC 21432), which consists of 10,537,932 bp (G+C content of 71.96%) with two linear chromosomes, 8983 protein-coding genes, 67 tRNAs and 6 rRNA operons. Genes related to biosynthesis of validamycin, valienamine and diverse secondary metabolites were detected in this genome. Genomic data is thus expected to considerably improve our understanding of how industrially important aminocyclitols are biosynthesized by microbial cells.
authors.affiliation: Microbial Resource Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea; Department of Bioinformatics, University of Science and Technology, Daejeon, Republic of Korea.
authors.forename: Sang-Heon
authors.initials: SH
authors.lastname: Lee
authors.affiliation: Microbial Resource Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea.
authors.forename: Hanna
authors.initials: H
authors.lastname: Choe
authors.affiliation: Microbial Resource Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea.
authors.forename: Kyung Sook
authors.initials: KS
authors.lastname: Bae
authors.affiliation: Microbial Resource Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea.
authors.forename: Doo-Sang
authors.initials: DS
authors.lastname: Park
authors.affiliation: Department of Biosciences, COMSATS Institute of Information Technology, Islamabad, Pakistan.
authors.forename: Arshan
authors.initials: A
authors.lastname: Nasir
authors.affiliation: Microbial Resource Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea; Department of Bioinformatics, University of Science and Technology, Daejeon, Republic of Korea. Electronic address: kmkim@kribb.re.kr.
authors.forename: Kyung Mo
authors.initials: KM
authors.lastname: Kim
mesh_terms: D001482:Base Composition; D059646:Genome Size; D016680:Genome, Bacterial; D007294:Inositol / Q000031:analogs & derivatives / Q000235:genetics / Q000378:metabolism; D064210:Secondary Metabolism; D012988:Soil Microbiology; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism*
chemical_list: D007294:Inositol; C003749:validamycins
keywords: Complete genome; PacBio sequencing; Streptomyces hygroscopicus subsp. limoneus; Validamycin