title: Asaia lannensis bacteremia in a 'needle freak' patient.
abstract: The genus Asaia has gained much interest lately owing to constant new species discoveries and its role as a potential opportunistic pathogen to humans. Here we describe a transient bacteremia due to Asaia lannensis in a patient with a psychiatric disorder (compulsive self-injection of different substances). Common phenotypic methods of identification failed to identify this organism, and only restriction fragment lenght polymorphism of PCR-amplified 16S rRNA gene allowed for proper identification. The isolate was highly resistant to most antibiotics. The paper also discusses the currently available medical literature, acknowledges the potential problems linked to the isolation of these strains and proposes an approach to species identification that can be applied in a clinical microbiology laboratory.
authors.affiliation: Clinical Microbiology Laboratory - IRCCS Arcispedale Santa Maria Nuova, Reggio Emilia, Italy.
authors.forename: Edoardo
authors.initials: E
authors.lastname: Carretto
authors.affiliation: Clinical Microbiology Laboratory - IRCCS Arcispedale Santa Maria Nuova, Reggio Emilia, Italy.
authors.forename: Rosa
authors.initials: R
authors.lastname: Visiello
authors.affiliation: Clinical Microbiology Laboratory - IRCCS Arcispedale Santa Maria Nuova, Reggio Emilia, Italy.
authors.forename: Marcellino
authors.initials: M
authors.lastname: Bardaro
authors.affiliation: Infectious Diseases - IRCCS Arcispedale Santa Maria Nuova, Reggio Emilia, Italy.
authors.forename: Simona
authors.initials: S
authors.lastname: Schivazappa
authors.affiliation: Microbiology & Virology Laboratory - Azienda Ospedaliera 'Papa Giovanni XXIII', Bergamo, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Vailati
authors.affiliation: Microbiology & Virology Laboratory - Azienda Ospedaliera 'Papa Giovanni XXIII', Bergamo, Italy.
authors.forename: Claudio
authors.initials: C
authors.lastname: Farina
authors.affiliation: Virology & Microbiology Laboratory - Fondazione IRCCS Policlinico San Matteo, Pavia, Italy.
authors.forename: Daniela
authors.initials: D
authors.lastname: Barbarini
mesh_terms: D016947:Acetobacteraceae / Q000145:classification / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D000328:Adult; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D016470:Bacteremia / Q000175:diagnosis* / Q000382:microbiology; D001431:Bacteriological Techniques / Q000379:methods; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial; D005260:Female; D016905:Gram-Negative Bacterial Infections / Q000175:diagnosis* / Q000382:microbiology; D006801:Humans; D001523:Mental Disorders / Q000150:complications*; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S
keywords: Asaia; Asaia lannensis; bacteremia; clinical significance; molecular identification