title: Downregulation of de Novo Fatty Acid Synthesis in Subcutaneous Adipose Tissue of Moderately Obese Women.
abstract: The purpose of this work was to evaluate the expression of fatty acid metabolism-related genes in human adipose tissue from moderately obese women. We used qRT-PCR and Western Blot to analyze visceral (VAT) and subcutaneous (SAT) adipose tissue mRNA expression involved in de novo fatty acid synthesis (ACC1, FAS), fatty acid oxidation (PPARα, PPARδ) and inflammation (IL6, TNFα), in normal weight control women (BMI < 25 kg/m², n = 35) and moderately obese women (BMI 30-38 kg/m², n = 55). In SAT, ACC1, FAS and PPARα mRNA expression were significantly decreased in moderately obese women compared to controls. The downregulation reported in SAT was more pronounced when BMI increased. In VAT, lipogenic-related genes and PPARα were similar in both groups. Only PPARδ gene expression was significantly increased in moderately obese women. As far as inflammation is concerned, TNFα and IL6 were significantly increased in moderate obesity in both tissues. Our results indicate that there is a progressive downregulation in lipogenesis in SAT as BMI increases, which suggests that SAT decreases the synthesis of fatty acid de novo during the development of obesity, whereas in VAT lipogenesis remains active regardless of the degree of obesity.
authors.affiliation: Grup de Recerca GEMMAIR (AGAUR)-Medicina Aplicada, Departament de Medicina i Cirurgia, Universitat Rovira i Virgili (URV), Institut d'Investigació Pere Virgili (IISPV), Mallafré Guasch, 4, 43007 Tarragona, Spain. esther.guiu@urv.cat.
authors.forename: Esther
authors.initials: E
authors.lastname: Guiu-Jurado
authors.affiliation: Grup de Recerca GEMMAIR (AGAUR)-Medicina Aplicada, Departament de Medicina i Cirurgia, Universitat Rovira i Virgili (URV), Institut d'Investigació Pere Virgili (IISPV), Mallafré Guasch, 4, 43007 Tarragona, Spain. tauguet.hj23.ics@gencat.cat.|Servei Medicina Interna, Hospital Universitari Joan XXIII Tarragona, Mallafré Guasch, 4, 43007 Tarragona, Spain. tauguet.hj23.ics@gencat.cat.
authors.forename: Teresa
authors.initials: T
authors.lastname: Auguet
authors.affiliation: Grup de Recerca GEMMAIR (AGAUR)-Medicina Aplicada, Departament de Medicina i Cirurgia, Universitat Rovira i Virgili (URV), Institut d'Investigació Pere Virgili (IISPV), Mallafré Guasch, 4, 43007 Tarragona, Spain. alba.berlanga@urv.cat.
authors.forename: Alba
authors.initials: A
authors.lastname: Berlanga
authors.affiliation: Grup de Recerca GEMMAIR (AGAUR)-Medicina Aplicada, Departament de Medicina i Cirurgia, Universitat Rovira i Virgili (URV), Institut d'Investigació Pere Virgili (IISPV), Mallafré Guasch, 4, 43007 Tarragona, Spain. gemma.aragones@iispv.cat.
authors.forename: Gemma
authors.initials: G
authors.lastname: Aragonès
authors.affiliation: Grup de Recerca GEMMAIR (AGAUR)-Medicina Aplicada, Departament de Medicina i Cirurgia, Universitat Rovira i Virgili (URV), Institut d'Investigació Pere Virgili (IISPV), Mallafré Guasch, 4, 43007 Tarragona, Spain. caguilar.hj23.ics@gencat.cat.
authors.forename: Carmen
authors.initials: C
authors.lastname: Aguilar
authors.affiliation: Servei de Cirurgia, Hospital Sant Joan de Reus, Departament de Medicina i Cirurgia, Universitat Rovira i Virgili (URV), Institut d'Investigació Pere Virgili (IISPV), Avinguda Doctor Josep Laporte, 2, 43204 Reus, Spain. fatima.sabench@urv.cat.
authors.forename: Fàtima
authors.initials: F
authors.lastname: Sabench
authors.affiliation: Grup de Recerca GEMMAIR (AGAUR)-Medicina Aplicada, Departament de Medicina i Cirurgia, Universitat Rovira i Virgili (URV), Institut d'Investigació Pere Virgili (IISPV), Mallafré Guasch, 4, 43007 Tarragona, Spain. sandra.armengol@urv.cat.
authors.forename: Sandra
authors.initials: S
authors.lastname: Armengol
authors.affiliation: Servei Medicina Interna, Hospital Universitari Joan XXIII Tarragona, Mallafré Guasch, 4, 43007 Tarragona, Spain. aporras.hj23.ics@gencat.cat.
authors.forename: José Antonio
authors.initials: JA
authors.lastname: Porras
authors.affiliation: Servei Medicina Interna, Hospital Universitari Joan XXIII Tarragona, Mallafré Guasch, 4, 43007 Tarragona, Spain. andreumano@gmail.com.
authors.forename: Andreu
authors.initials: A
authors.lastname: Martí
authors.affiliation: Servei de Cirurgia, Hospital Universitari Joan XXIII Tarragona, Mallafré Guasch, 4, 43007 Tarragona, Spain. rjorba.hj23.ics@gencat.cat.
authors.forename: Rosa
authors.initials: R
authors.lastname: Jorba
authors.affiliation: Servei de Cirurgia, Hospital Sant Joan de Reus, Departament de Medicina i Cirurgia, Universitat Rovira i Virgili (URV), Institut d'Investigació Pere Virgili (IISPV), Avinguda Doctor Josep Laporte, 2, 43204 Reus, Spain. mhernandezg@grupsagessa.com.
authors.forename: Mercè
authors.initials: M
authors.lastname: Hernández
authors.affiliation: Servei de Cirurgia, Hospital Sant Joan de Reus, Departament de Medicina i Cirurgia, Universitat Rovira i Virgili (URV), Institut d'Investigació Pere Virgili (IISPV), Avinguda Doctor Josep Laporte, 2, 43204 Reus, Spain. ddelcastillo@grupsagessa.com.
authors.forename: Daniel
authors.initials: D
authors.lastname: del Castillo
authors.affiliation: Grup de Recerca GEMMAIR (AGAUR)-Medicina Aplicada, Departament de Medicina i Cirurgia, Universitat Rovira i Virgili (URV), Institut d'Investigació Pere Virgili (IISPV), Mallafré Guasch, 4, 43007 Tarragona, Spain. crichart.hj23.ics@gencat.cat.|Servei Medicina Interna, Hospital Universitari Joan XXIII Tarragona, Mallafré Guasch, 4, 43007 Tarragona, Spain. crichart.hj23.ics@gencat.cat.
authors.forename: Cristóbal
authors.initials: C
authors.lastname: Richart
mesh_terms: D015331:Cohort Studies; D015536:Down-Regulation / Q000235:genetics*; D005227:Fatty Acids / Q000096:biosynthesis; D005260:Female; D005947:Glucose / Q000378:metabolism; D006801:Humans; D007249:Inflammation / Q000235:genetics; D050152:Intra-Abdominal Fat / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D050155:Lipogenesis / Q000235:genetics; D008875:Middle Aged; D009765:Obesity / Q000235:genetics*; D010084:Oxidation-Reduction; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D050151:Subcutaneous Fat / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology
chemical_list: D005227:Fatty Acids; D012333:RNA, Messenger; D005947:Glucose
keywords: adipose tissue; de novo fatty acid synthesis; fatty acid metabolism; moderate obesity