title: Running exercise enhances motor functional recovery with inhibition of dendritic regression in the motor cortex after collagenase-induced intracerebral hemorrhage in rats.
abstract: Rehabilitative approaches benefit motor functional recovery after stroke and relate to neuronal plasticity. We investigated the effects of a treadmill running exercise on the motor functional recovery and neuronal plasticity after collagenase-induced striatal intracerebral hemorrhage (ICH) in rats. Male Wistar rats were injected with type IV collagenase into the left striatum to induce ICH. Sham-operated animals were injected with saline instead of collagenase. The animals were randomly assigned to the sham control (SC), the sham exercise (SE), the ICH control (IC), or the ICH exercise (IE) group. The exercise groups were forced to run on a treadmill at a speed of 9 m/min for 30 min/day between days 4 and 14 after surgery. Behavioral tests were performed using a motor deficit score, a beam-walking test and a cylinder test. At fifteen days after surgery, the animals were sacrificed, and their brains were removed. The motor function of the IE group significantly improved compared with the motor function of the IC group. No significant differences in cortical thickness were found between the groups. The IC group had fewer branches and shorter dendrite lengths compared with the sham groups. However, dendritic branches and lengths were not significantly different between the IE and the other groups. Tropomyosin-related kinase B (TrkB) expression levels increased in the IE compared with IC group, but no significant differences in other protein (brain-derived neurotrophic factor, BDNF; Nogo-A; Rho-A/Rho-associated protein kinase 2, ROCK2) expression levels were found between the groups. These results suggest that improved motor function after a treadmill running exercise after ICH may be related to the prevention of dendritic regression due to TrkB upregulation.
authors.affiliation: Department of Physical Therapy, Program in Physical and Occupational Therapy, Nagoya University Graduate School of Medicine, Nagoya, Aichi, Japan; Department of Rehabilitation, National Hospital Organization Higashi Nagoya National Hospital, Nagoya, Aichi, Japan.
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Takamatsu
authors.affiliation: Department of Physical Therapy, Program in Physical and Occupational Therapy, Nagoya University Graduate School of Medicine, Nagoya, Aichi, Japan; Department of Physical Therapy, Niigata University of Health and Welfare, Niigata, Japan.
authors.forename: Keigo
authors.initials: K
authors.lastname: Tamakoshi
authors.affiliation: Department of Physical Therapy, Program in Physical and Occupational Therapy, Nagoya University Graduate School of Medicine, Nagoya, Aichi, Japan.
authors.forename: Yuya
authors.initials: Y
authors.lastname: Waseda
authors.affiliation: Department of Physical Therapy, Program in Physical and Occupational Therapy, Nagoya University Graduate School of Medicine, Nagoya, Aichi, Japan. Electronic address: ishida@met.nagoya-u.ac.jp.
authors.forename: Kazuto
authors.initials: K
authors.lastname: Ishida
mesh_terms: D000818:Animals; D002543:Cerebral Hemorrhage / Q000473:pathology / Q000503:physiopathology / Q000628:therapy*; D017364:Collagenases; D003712:Dendrites / Q000473:pathology / Q000502:physiology; D004195:Disease Models, Animal; D005081:Exercise Therapy / Q000379:methods; D007839:Functional Laterality; D008297:Male; D009043:Motor Activity / Q000502:physiology*; D009044:Motor Cortex / Q000473:pathology / Q000503:physiopathology*; D009185:Myelin Proteins / Q000378:metabolism; D000070798:Nogo Proteins; D011897:Random Allocation; D017208:Rats, Wistar; D020813:Receptor, trkB / Q000378:metabolism; D020127:Recovery of Function / Q000502:physiology*; D012420:Running / Q000502:physiology*; D016896:Treatment Outcome; D015854:Up-Regulation / Q000502:physiology; D054460:rho-Associated Kinases / Q000378:metabolism
chemical_list: D009185:Myelin Proteins; D000070798:Nogo Proteins; C000603197:Rtn4 protein, rat; D020813:Receptor, trkB; C580818:ROCK2 protein, rat; D054460:rho-Associated Kinases; D017364:Collagenases
keywords: Dendrite; Intracerebral hemorrhage; Motor functional recovery; Running exercise; Tropomyosin-related kinase B