title: Active cigarette smoking and the risk of breast cancer at the level of N-acetyltransferase 2 (NAT2) gene polymorphisms.
abstract: The aim of our study was to assess the correlation between the tobacco exposure and NAT2 gene (rs1041983 C/T, rs1801280 T/C, rs1799930 G/A) polymorphisms in association with breast cancer development. We wanted to determine the prognostic clinical importance of these polymorphisms in association with smoking and breast cancer. For the detection of possible association between smoking, NAT2 gene polymorphisms, and the risk of breast cancer, we designed a case-controlled study with 198 patients enrolled, 98 breast cancer patients and 100 healthy controls. Ten milliliters of peripheral blood from the cubital vein was withdrawn from every patient. The HRM (high resolution melting) analysis was used for the detection of three abovementioned NAT2 gene polymorphisms. When comparing a group of women smoking more than 5 cigarettes a day with the patients smoking fewer than 5 cigarettes a day, we found out that if women were the carriers of aberrant AA genotype for rs1799930, the first group of women had higher risk of breast carcinoma than the second group. If patients were the carriers of aberrant TT genotype for rs1041983, for rs1801280CC genotype, and rs1799930AA genotype and they smoked more than 5 cigarettes a day, they had higher risk of malignant breast disease than never-smoking women. Our results confirm the hypothesis that NAT2 gene polymorphisms (rs1041983 C/T, rs1801280 T/C, and rs1799930 G/A) in association with long-period active smoking could be the possible individual risk-predicting factors for breast cancer development in the population of Slovak women.
authors.affiliation: Department of Obstetrics and Gynecology, Jessenius Faculty of Medicine, Comenius University in Bratislava, Martin, Slovakia. petra.sumichrastova@gmail.com.
authors.forename: Petra
authors.initials: P
authors.lastname: Kasajova
authors.affiliation: Biomedical Center Martin, Comenius University in Bratislava, Martin, Slovakia.
authors.forename: Veronika
authors.initials: V
authors.lastname: Holubekova
authors.affiliation: Biomedical Center Martin, Comenius University in Bratislava, Martin, Slovakia.
authors.forename: Andrea
authors.initials: A
authors.lastname: Mendelova
authors.affiliation: Biomedical Center Martin, Comenius University in Bratislava, Martin, Slovakia.
authors.forename: Zora
authors.initials: Z
authors.lastname: Lasabova
authors.affiliation: Department of Obstetrics and Gynecology, Jessenius Faculty of Medicine, Comenius University in Bratislava, Martin, Slovakia.
authors.forename: Pavol
authors.initials: P
authors.lastname: Zubor
authors.affiliation: Department of Obstetrics and Gynecology, Jessenius Faculty of Medicine, Comenius University in Bratislava, Martin, Slovakia.
authors.forename: Erik
authors.initials: E
authors.lastname: Kudela
authors.affiliation: Department of Obstetrics and Gynecology, Jessenius Faculty of Medicine, Comenius University in Bratislava, Martin, Slovakia.
authors.forename: Kristina
authors.initials: K
authors.lastname: Biskupska-Bodova
authors.affiliation: Department of Obstetrics and Gynecology, Jessenius Faculty of Medicine, Comenius University in Bratislava, Martin, Slovakia.
authors.forename: Jan
authors.initials: J
authors.lastname: Danko
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001191:Arylamine N-Acetyltransferase / Q000235:genetics*; D001943:Breast Neoplasms / Q000209:etiology* / Q000235:genetics; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D008875:Middle Aged; D011110:Polymorphism, Genetic; D011379:Prognosis; D012307:Risk Factors; D012907:Smoking / Q000009:adverse effects*; D014026:Nicotiana
chemical_list: D001191:Arylamine N-Acetyltransferase; C478900:NAT2 protein, human
keywords: Breast cancer; NAT2 gene; Polymorphisms; Smoking