title: Noscapine protects OLN-93 oligodendrocytes from ischemia-reperfusion damage: Calcium and nitric oxide involvement.
abstract: This study was carried out to evaluate the effects of noscapine, a benzylisoquinoline alkaloid from opium poppy, on oligodendrocyte during ischemia/reperfusion-induced excitotoxic injury. Changes in intracellular calcium levels due to chemical ischemia and nitric oxide (NO) production during ischemia/reperfusion were evaluated as the hallmarks of ischemia-derived excitotoxic event. OLN-93 cell line (a permanent immature rat oligodendrocyte) was used as a model of oligodendrocyte. 30- or 60-minute-oxygen-glucose deprivation/24 hours reperfusion were used to induce excitotoxicity. MTT (3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide) assay was used to evaluate cell viability. Ratiometric fluorescence microscopy using Ca(2+)-sensitive indicator Fura-2/AM was utilized to assess intracellular calcium levels. NO production was evaluated by Griess method. Noscapine (4 μM) significantly attenuated intracellular Ca(2+) elevation (P < 0.001). Also, noscapine significantly decreased NO production during a 30-minute oxygen-glucose deprivation/reperfusion (P < 0.01). The inhibitory effect of noscapine (4 μM) on intracellular Ca(2+) was greater than ionotropic glutamate receptors antagonists. Noscapine is protective against ischemia/reperfusion-induced excitotoxic injury in OLN-93 oligodendrocyte. This protective effect seems to be related to attenuation of intracellular Ca(2+) overload and NO production.
authors.affiliation: Faculty of Medicine, Iran University of Medical Sciences , Tehran , Iran.
authors.forename: S
authors.initials: S
authors.lastname: Nadjafi
authors.affiliation: Faculty of Medicine, Iran University of Medical Sciences , Tehran , Iran.
authors.forename: S-A
authors.initials: SA
authors.lastname: Ebrahimi
authors.affiliation: Faculty of Medicine, Iran University of Medical Sciences , Tehran , Iran.
authors.forename: N
authors.initials: N
authors.lastname: Rahbar-Roshandel
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D009665:Noscapine / Q000494:pharmacology*; D009836:Oligodendroglia; D051381:Rats; D015427:Reperfusion Injury / Q000517:prevention & control*
chemical_list: D009569:Nitric Oxide; D009665:Noscapine; D002118:Calcium
keywords: OLN-93 cell line; calcium; nitric oxide; noscapine; oligodendrocyte; oxygen-glucose-deprivation