title: Recent advances and versatility of MAGE towards industrial applications.
abstract: The genome engineering toolkit has expanded significantly in recent years, allowing us to study the functions of genes in cellular networks and assist in over-production of proteins, drugs, chemicals and biofuels. Multiplex automated genome engineering (MAGE) has been recently developed and gained more scientific interest towards strain engineering. MAGE is a simple, rapid and efficient tool for manipulating genes simultaneously in multiple loci, assigning genetic codes and integrating non-natural amino acids. MAGE can be further expanded towards the engineering of fast, robust and over-producing strains for chemicals, drugs and biofuels at industrial scales.
authors.affiliation: Institute of Systems and Synthetic Biology, Université d'Évry Val d'Essonne, Genopole Campus 1, Batiment Genavenir 6, 5 rue Henri Desbruères, 91030 Évry, France.
authors.forename: Vijai
authors.initials: V
authors.lastname: Singh
authors.affiliation: Institute of Systems and Synthetic Biology, Université d'Évry Val d'Essonne, Genopole Campus 1, Batiment Genavenir 6, 5 rue Henri Desbruères, 91030 Évry, France.
authors.forename: Darren
authors.initials: D
authors.lastname: Braddick
keywords: Biofuels; Chemicals; Drugs; Genome; Multiplex automated genome engineering