title: Purification, Characterization of L-Methioninase from Candida tropicalis, and Its Application as an Anticancer.
abstract: The aim of the present study is to purify L-methioninase from Candida tropicalis 34.19-fold with 27.98% recovery after ion exchange chromatography followed by gel filtration. The purified enzyme revealed a single band on SDS-PAGE gel with a molecular weight of 46 KDa. Its optimum temperature was 45 to 55 and thermal stability was 55°C for 15 min. The enzyme had optimum pH at 6.5 and stability at a pH range of 5.5 to 7.0 for 24 hr. The maximum activity was observed with substrate concentration of 30 µM and Km was 0.5 mM. The enzyme was strongly inhibited by Cd(+2) and Cu(+2) while it was enhanced by Na(+), Ni(+2), and Mg(+2) at 10 mM while Ca(+2) had slight activation at 20 mM. In addition, the potential application of the L-methioninase as an anticancer agent against various types of tumor cell lines is discussed.
authors.affiliation: National Research Centre, P.O. Box 12622, Dokki, Giza, Egypt.
authors.forename: Mohsen Helmy
authors.initials: MH
authors.lastname: Selim
authors.affiliation: Faculty of Science, Ain Shams University, Cairo, Egypt.
authors.forename: El-Zahraa
authors.initials: el-Z
authors.lastname: Karm Eldin
authors.affiliation: National Research Centre, P.O. Box 12622, Dokki, Giza, Egypt.
authors.forename: Moataza Mahmoud
authors.initials: MM
authors.lastname: Saad
authors.affiliation: National Research Centre, P.O. Box 12622, Dokki, Giza, Egypt.
authors.forename: El-Sayed Eliwa
authors.initials: el-SE
authors.lastname: Mostafa
authors.affiliation: Faculty of Science, Ain Shams University, Cairo, Egypt.
authors.forename: Yosrea Hassan
authors.initials: YH
authors.lastname: Shetia
authors.affiliation: National Research Centre, P.O. Box 12622, Dokki, Giza, Egypt.
authors.forename: Amany Ahmed Hassabo
authors.initials: AA
authors.lastname: Anise