title: Efficacy of feed additives against Campylobacter in live broilers during the entire rearing period.
abstract: Poultry meat is the major source of human campylobacteriosis, the most frequently reported zoonosis in the EU. The prevalence of Campylobacter colonization in European broiler flocks is 71%. Despite considerable efforts, there is still no effective strategy available to prevent or reduce Campylobacter colonization in broilers. This study tested a wide variety of feed additives to reduce Campylobacter shedding in primary poultry production. Twelve additives containing organic or fatty acids, monoglycerides, plant extracts, prebiotics, or probiotics were tested. For each additive, broilers contaminated with Campylobacter jejuni were fed with an additive free diet (control group) or with a supplemented diet (treated group) and Campylobacter loads compared at three sampling times. No treatment was able to prevent broiler colonization by Campylobacter, and there was a high degree of variation in contamination among the birds. At 14 d of age, eight treatments significantly decreased the colonization level compared to the control group by a maximum of 2 log10 CFU/g. At 35 d of age, three of these treatments still had a significant effect with a maximum reduction of 1.88 log10 CFU/g for a probiotic. At 42 d of age, only one short-chain fatty acid was still significantly efficient with a mean reduction over 2 log10 CFU/g. In addition, a probiotic and a prebiotic-like compound significantly decreased the contamination by a maximum of 3 log10 CFU/g, only at the 42-d sampling period. This study gives promising results regarding the use of feed additives to reduce Campylobacter infection in flocks. Nevertheless, a global approach, combining intervention measures at the different steps of the broiler meat production chain could have a greater impact on the reduction of public health risk.
authors.affiliation: ANSES, Ploufragan-Plouzané Laboratory, Unit of Hygiene and Quality of Poultry and Pork Products, BP53, 22440 Ploufragan, France and European University of Brittany, France muriel.guyard@anses.fr.
authors.forename: M
authors.initials: M
authors.lastname: Guyard-Nicodème
authors.affiliation: ANSES, Ploufragan-Plouzané Laboratory, Department of Poultry Experimentation, BP53, 22440 Ploufragan, France and European University of Brittany, France.
authors.forename: A
authors.initials: A
authors.lastname: Keita
authors.affiliation: ANSES, Ploufragan-Plouzané Laboratory, Unit of Hygiene and Quality of Poultry and Pork Products, BP53, 22440 Ploufragan, France and European University of Brittany, France.
authors.forename: S
authors.initials: S
authors.lastname: Quesne
authors.affiliation: ANSES, Ploufragan-Plouzané Laboratory, Department of Poultry Experimentation, BP53, 22440 Ploufragan, France and European University of Brittany, France.
authors.forename: M
authors.initials: M
authors.lastname: Amelot
authors.affiliation: ANSES, Ploufragan-Plouzané Laboratory, Unit of Hygiene and Quality of Poultry and Pork Products, BP53, 22440 Ploufragan, France and European University of Brittany, France.
authors.forename: T
authors.initials: T
authors.lastname: Poezevara
authors.affiliation: ANSES, Ploufragan-Plouzané Laboratory, Department of Poultry Experimentation, BP53, 22440 Ploufragan, France and European University of Brittany, France.
authors.forename: B
authors.initials: B
authors.lastname: Le Berre
authors.affiliation: IMASDE Agroalimentaria, S.L., Calle Nápoles, 3 28224 Pozuelo de Alarcón, Madrid, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Sánchez
authors.affiliation: NEPLUVI, Kokermolen11, Houten, 3994 DG, Netherlands.
authors.forename: P
authors.initials: P
authors.lastname: Vesseur
authors.affiliation: Propollo, Calle Diego de León 33, 28006 Madrid, Spain.
authors.forename: Á
authors.initials: Á
authors.lastname: Martín
authors.affiliation: IMASDE Agroalimentaria, S.L., Calle Nápoles, 3 28224 Pozuelo de Alarcón, Madrid, Spain.
authors.forename: P
authors.initials: P
authors.lastname: Medel
authors.affiliation: ANSES, Ploufragan-Plouzané Laboratory, Unit of Hygiene and Quality of Poultry and Pork Products, BP53, 22440 Ploufragan, France and European University of Brittany, France.
authors.forename: Marianne
authors.initials: M
authors.lastname: Chemaly
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000822:Animal Husbandry; D000818:Animals; D057138:Bacterial Shedding; D002169:Campylobacter Infections / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary*; D016123:Campylobacter jejuni / Q000502:physiology*; D002432:Cecum / Q000382:microbiology; D002645:Chickens; D004032:Diet / Q000662:veterinary; D019587:Dietary Supplements; D000069196:Gastrointestinal Microbiome; D011201:Poultry Diseases / Q000382:microbiology / Q000517:prevention & control*; D011897:Random Allocation
keywords: Campylobacter jejuni; broiler; control measure; feed additive