title: Differential Contribution of BLT1 and BLT2 to Leukotriene B4-Induced Human NK Cell Cytotoxicity and Migration.
abstract: Accumulating evidence indicates that leukotriene B4 (LTB4) via its receptors BLT1 and/or BLT2 (BLTRs) could have an important role in regulating infection, tumour progression, inflammation, and autoimmune diseases. In the present study, we showed that LTB4 not only augments cytotoxicity by NK cells but also induces their migration. We found that approximately 30% of fresh NK cells express BLT1, 36% express BLT2, and 15% coexpress both receptors. The use of selective BLTR antagonists indicated that BLT1 was involved in both LTB4-induced migration and cytotoxicity, whereas BLT2 was involved exclusively in NK cell migration, but only in response to higher concentrations of LTB4. BLT1 and BLT2 expression increased after activation of NK cells with IL-2 and IL-15. These changes of BLTR expression by cytokines were reflected in enhanced NK cell responses to LTB4. Our findings suggest that BLT1 and BLT2 play differential roles in LTB4-induced modulation of NK cell activity.
authors.affiliation: Division of Immunology, Department of Paediatrics, Faculty of Medicine and Health Sciences, Université de Sherbrooke, Sherbrooke, QC, Canada J1H 5N4.
authors.forename: Meng
authors.initials: M
authors.lastname: Wang
authors.affiliation: Division of Immunology, Department of Paediatrics, Faculty of Medicine and Health Sciences, Université de Sherbrooke, Sherbrooke, QC, Canada J1H 5N4.
authors.forename: Nermine
authors.initials: N
authors.lastname: Mostafa El-Maghraby
authors.affiliation: Division of Immunology, Department of Paediatrics, Faculty of Medicine and Health Sciences, Université de Sherbrooke, Sherbrooke, QC, Canada J1H 5N4.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Turcotte
authors.affiliation: Division of Immunology, Department of Paediatrics, Faculty of Medicine and Health Sciences, Université de Sherbrooke, Sherbrooke, QC, Canada J1H 5N4.
authors.forename: Marek
authors.initials: M
authors.lastname: Rola-Pleszczynski
authors.affiliation: Division of Immunology, Department of Paediatrics, Faculty of Medicine and Health Sciences, Université de Sherbrooke, Sherbrooke, QC, Canada J1H 5N4.
authors.forename: Jana
authors.initials: J
authors.lastname: Stankova
mesh_terms: D002465:Cell Movement / Q000187:drug effects*; D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic / Q000187:drug effects*; D006801:Humans; D019409:Interleukin-15 / Q000494:pharmacology; D007376:Interleukin-2 / Q000494:pharmacology; D007694:Killer Cells, Natural / Q000187:drug effects* / Q000276:immunology; D007975:Leukotriene B4 / Q000494:pharmacology*; D012333:RNA, Messenger / Q000032:analysis; D018102:Receptors, Leukotriene B4 / Q000032:analysis / Q000235:genetics / Q000502:physiology*
chemical_list: D019409:Interleukin-15; D007376:Interleukin-2; C513240:LTB4R protein, human; C485878:LTB4R2 protein, human; D012333:RNA, Messenger; D018102:Receptors, Leukotriene B4; D007975:Leukotriene B4