title: Selection of lactic acid bacteria strains for the hydrolysis of allergenic proteins of wheat flour.
abstract: BACKGROUND
  Wheat flour is one of the most common causative agents of food allergy. The study presents the selection and characterization of lactic acid bacteria (LAB) strains capable of hydrolyzing/modifying allergenic proteins of wheat flour. Hydrolysis of wheat proteins was determined with sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting with sera from patients with food allergy to gluten.
  
  
  RESULTS
  The analysis of electrophoretic profiles of protein extracted from sourdough shows the capability of selected LAB strains for proteolytic degradation of wheat proteins that belong to two factions: albumin/globulin (hydrolysis of 13 polypeptides with a molecular weight between 103 and 22 kDa); and gliadin (seven polypeptides with a molecular weight between 39 and 24 kDa). All analyzed strains were capable of hydrolyzing some IgE-binding epitopes of wheat allergens. The lack of such changes in control samples indicates that they were induced rather by the proteolytic activity of bacterial strains than endogenous enzymes of wheat flour. The gluten proteins were susceptible to hydrolysis by sequential digestion with pepsin and trypsin.
  
  
  CONCLUSIONS
  The selected strains exhibit proteolytic activity, which leads to a reduction in allergenicity of wheat sourdoughs. These strains may be applied as specific starter cultures to prepare bakery products of special nutritional use. © 2015 Society of Chemical Industry.
authors.affiliation: Prof. Wacław Dąbrowski Institute of Agricultural and Food Biotechnology, Department of Technology Fermentation, Rakowiecka 36 St, 02-532 Warsaw, Poland.
authors.forename: Ilona
authors.initials: I
authors.lastname: Stefańska
authors.affiliation: Prof. Wacław Dąbrowski Institute of Agricultural and Food Biotechnology, Department of Technology Fermentation, Rakowiecka 36 St, 02-532 Warsaw, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Piasecka-Jóźwiak
authors.affiliation: Prof. Wacław Dąbrowski Institute of Agricultural and Food Biotechnology, Department of Technology Fermentation, Rakowiecka 36 St, 02-532 Warsaw, Poland.
authors.forename: Danuta
authors.initials: D
authors.lastname: Kotyrba
authors.affiliation: Faculty of Horticulture, Biotechnology and Landscape Architecture, Warsaw University of Life Sciences, 02-787 Warsaw, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Kolenda
authors.affiliation: Prof. Wacław Dąbrowski Institute of Agricultural and Food Biotechnology, Department of Technology Fermentation, Rakowiecka 36 St, 02-532 Warsaw, Poland.
authors.forename: Krystyna M
authors.initials: KM
authors.lastname: Stecka
mesh_terms: D000418:Albumins / Q000009:adverse effects / Q000737:chemistry / Q000378:metabolism; D052179:Antigens, Plant / Q000009:adverse effects / Q000737:chemistry / Q000378:metabolism*; D001939:Bread / Q000009:adverse effects / Q000032:analysis* / Q000382:microbiology; D055050:Diet, Gluten-Free; D000939:Epitopes / Q000009:adverse effects / Q000378:metabolism; D005285:Fermentation; D005433:Flour / Q000009:adverse effects / Q000032:analysis* / Q000382:microbiology; D005903:Gliadin / Q000009:adverse effects / Q000737:chemistry / Q000378:metabolism; D005916:Globulins / Q000009:adverse effects / Q000737:chemistry / Q000378:metabolism; D005983:Glutens / Q000009:adverse effects / Q000378:metabolism*; D006801:Humans; D056546:Lactobacillales / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D008970:Molecular Weight; D014674:Plant Proteins, Dietary / Q000009:adverse effects / Q000737:chemistry / Q000378:metabolism*; D011044:Poland; D059748:Proteolysis; D012639:Seeds / Q000009:adverse effects / Q000737:chemistry / Q000382:microbiology; D013045:Species Specificity; D014908:Triticum / Q000009:adverse effects / Q000737:chemistry / Q000382:microbiology; D021182:Wheat Hypersensitivity / Q000178:diet therapy / Q000276:immunology
chemical_list: D000418:Albumins; D052179:Antigens, Plant; D000939:Epitopes; D005916:Globulins; D014674:Plant Proteins, Dietary; D005983:Glutens; D005903:Gliadin
keywords: food allergy; gluten; lactic acid bacteria; proteolysis; sourdough; wheat allergen