title: Novel evidence that pituitary gonadotropins directly stimulate human leukemic cells-studies of myeloid cell lines and primary patient AML and CML cells.
abstract: We recently reported that normal hematopoietic stem cells express functional pituitary sex hormone (SexH) receptors. Here we report for the first time that pituitary-secreted gonadotrophins stimulate migration, adhesion, and proliferation of several human myeloid and lymphoid leukemia cell lines. Similar effects were observed after stimulation of human leukemic cell lines by gonadal SexHs. This effect seems to be direct, as the SexH receptors expressed by leukemic cells responded to stimulation by phosphorylation of MAPKp42/44 and AKTser473. Furthermore, in parallel studies we confirmed that human primary patient-derived AML and CML blasts also express several functional SexH receptors. These results shed more light on the potential role of SexHs in leukemogenesis and, in addition, provide further evidence suggesting a developmental link between hematopoiesis and the germline.
authors.affiliation: Stem Cell Institute at James Graham Brown Cancer Center, University of Louisville, KY, USA.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Abdelbaset-Ismail
authors.affiliation: Pomeranian Medical University, Szczecin, Poland.
authors.forename: Sylwia
authors.initials: S
authors.lastname: Borkowska
authors.affiliation: Department of Hematology, University of Alberta, Edmonton, Canada.
authors.forename: Anna
authors.initials: A
authors.lastname: Janowska-Wieczorek
authors.affiliation: Transfusion Medicine, Medical Faculty Carl Gustav Carus - Technische Universtität Dresden, German Red Cross Blood Donation Service North East, Dresden, Germany.
authors.forename: Torsten
authors.initials: T
authors.lastname: Tonn
authors.affiliation: Stem Cell Institute at James Graham Brown Cancer Center, University of Louisville, KY, USA.
authors.forename: Cesar
authors.initials: C
authors.lastname: Rodriguez
authors.affiliation: Department of Regenerative Medicine and Immune Regulation, Medical University of Bialystok, Bialystok, Poland.
authors.forename: Marcin
authors.initials: M
authors.lastname: Moniuszko
authors.affiliation: Department of Hematology, Medical University of Bialystok, Bialystok, Poland.
authors.forename: Lukasz
authors.initials: L
authors.lastname: Bolkun
authors.affiliation: Department of Hematology, Medical University of Bialystok, Bialystok, Poland.
authors.forename: Janusz
authors.initials: J
authors.lastname: Koloczko
authors.affiliation: Department of Regenerative Medicine and Immune Regulation, Medical University of Bialystok, Bialystok, Poland.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Eljaszewicz
authors.affiliation: Stem Cell Institute at James Graham Brown Cancer Center, University of Louisville, KY, USA.
authors.forename: Janina
authors.initials: J
authors.lastname: Ratajczak
authors.affiliation: Stem Cell Institute at James Graham Brown Cancer Center, University of Louisville, KY, USA.|Department of Regenerative Medicine Medical University of Warsaw, Warsaw, Poland.
authors.forename: Mariusz Z
authors.initials: MZ
authors.lastname: Ratajczak
authors.affiliation: Stem Cell Institute at James Graham Brown Cancer Center, University of Louisville, KY, USA.|Department of Regenerative Medicine Medical University of Warsaw, Warsaw, Poland.
authors.forename: Magda
authors.initials: M
authors.lastname: Kucia
mesh_terms: D002448:Cell Adhesion / Q000502:physiology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation / Q000502:physiology; D005640:Follicle Stimulating Hormone / Q000494:pharmacology*; D006065:Gonadotropins, Pituitary / Q000378:metabolism*; D006801:Humans; D019169:Jurkat Cells; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000378:metabolism*; D015470:Leukemia, Myeloid, Acute / Q000378:metabolism*; D007986:Luteinizing Hormone / Q000494:pharmacology*; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D022423:Myeloid Cells / Q000378:metabolism; D010766:Phosphorylation; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D011979:Receptors, Pituitary Hormone / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D006065:Gonadotropins, Pituitary; D011979:Receptors, Pituitary Hormone; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D051057:Proto-Oncogene Proteins c-akt; D020928:Mitogen-Activated Protein Kinases
keywords: FSH; LH; germ line; leukemia; lymphoma