title: Potential use of acetylcholinesterase, glutathione-S-transferase and metallothionein for assessment of contaminated sediment in tropical chironomid, Chironomus javanus.
abstract: Heavy metals and organophosphorus insecticide is known to act as disruptors for the enzyme system, leading to physiologic disorders. The present study was conducted to investigate the potential use of these enzymes as biomarkers in assessment of contaminated sediments on tropical chironomid species. Acetylcholinesterase (AChE), glutathione-S-transferase (GST) and metallothionein (MT) activity was measured in the fourth-instar chironomid larvae, Chironomus javanus, Kieffer, after either 48-hr or 96-hr exposure to organophosphorus insecticide, chlorpyrifos (0.01- 0.25 mg kg(-1)) or heavy metal cadmium (0.1-25 mg kg(-1)). Exposure to chlorpyrifos (0.01 mg kg(-1)) at 48 and 96 hr significantly of AChE activity (64.2%-85.9%) and induced GST activity (33.9-63.8%) when compared with control (P < 0.05). Moreover, exposure to cadmium (0.1 mg kg(-1)) at 48 and 96 hr also showed significant increas GST activity (11.7-40%) and MT level (9.0%-70.5%) when compared with control (P < 0.05). The results indicated the impact of enzyme activity on chlorpyrifos and cadmium contamination. Activity of AChE, GST and MT could serve as potential biomarkers for assessment and biomonitoring the effects of insecticide and heavy metal contamination in tropical aquatic ecosystems.
authors.forename: A
authors.initials: A
authors.lastname: Somparn
authors.forename: C B
authors.initials: CB
authors.lastname: Iwai
authors.forename: B
authors.initials: B
authors.lastname: Noller
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D002683:Chironomidae / Q000187:drug effects*; D002800:Cholinesterase Inhibitors / Q000737:chemistry / Q000633:toxicity; D019015:Geologic Sediments / Q000737:chemistry*; D005982:Glutathione Transferase / Q000378:metabolism*; D007306:Insecticides / Q000737:chemistry / Q000633:toxicity; D008668:Metallothionein / Q000378:metabolism*; D019216:Metals, Heavy / Q000737:chemistry / Q000633:toxicity; D014874:Water Pollutants, Chemical / Q000737:chemistry / Q000633:toxicity*
chemical_list: D002800:Cholinesterase Inhibitors; D007306:Insecticides; D019216:Metals, Heavy; D014874:Water Pollutants, Chemical; D008668:Metallothionein; D005982:Glutathione Transferase; D000110:Acetylcholinesterase