title: Methyl-β-Cyclodextrin /Cetyltrimethyl Ammonium Bromide Synergistic Sensitized Fluorescence Method for the Determination of Levofloxacin.
abstract: A novel method of methyl-β-cyclodextrin (methyl-β-CD) and cetyltrimethyl ammonium bromide (CTAB) synergistic sensitized fluorescence analysis to determine levofloxacin (LVFX) was developed. The results were shown that the fluorescence intensity of LVFX was increased a lot in the system of methyl-β-cyclodextrin-CTAB medium. Under the conditions of λ(ex/em )= 330/507 nm and pH 4.5, the linear range and the detection limit for LVFX were found to be 0.040 ~ 4.0 μg/mL and 0.3 ng/mL, respectively. The mechanism of sensitized fluorescence method was discussed by the solubilization capacity and the microenvironment of medium. The proposed method has been applied for the determination of LVFX in eye drops real samples and human serum with satisfactory recovery.
authors.affiliation: College of Chemistry & Chemical Engineering, Yangzhou University, Yangzhou, 225002, People's Republic of China.
authors.forename: Qiuyi
authors.initials: Q
authors.lastname: Ren
authors.affiliation: College of Chemistry & Chemical Engineering, Yangzhou University, Yangzhou, 225002, People's Republic of China. xszhu@yzu.edu.cn.
authors.forename: Xiashi
authors.initials: X
authors.lastname: Zhu
mesh_terms: D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000737:chemistry*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D064368:Healthy Volunteers; D006801:Humans; D006863:Hydrogen-Ion Concentration; D064704:Levofloxacin / Q000032:analysis*; D057230:Limit of Detection; D009883:Ophthalmic Solutions / Q000032:analysis*; D044967:Serum / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D002593:Cetrimonium Compounds; D005456:Fluorescent Dyes; D009883:Ophthalmic Solutions; D047392:beta-Cyclodextrins; C108732:methyl-beta-cyclodextrin; D064704:Levofloxacin; D000077286:Cetrimonium
keywords: CTAB; Fluorescence spectrometry; Levofloxacin; Methyl-β-cyclodextrin; Sensitized