title: Contribution of ellagic acid on the antioxidant potential of medicinal plant Epilobium hirsutum.
abstract: In the present study, the possible role of ellagic acid (EA) on antioxidant potential of Epilobium hirsutum (EH) in rat liver was investigated. Wistar rats were intraperitoneally treated with 37.5 mg/kg of EH and 10 mg/kg of EA for 9 days. Effects of EH and EA on antioxidant [glutathione peroxidase (GPx) and superoxide dismutases (SOD)] and Phase II [NADPH quinone oxidoreductase 1 (NQO1) and glutathione S-transferases (GSTs)] enzyme activities, as well as protein and mRNA expressions of those, were investigated. Polyphenolic content of EH was determined by LC-MS/MS analysis. EH and EA injection to rats resulted in a significant increase of NQO1 (3.6-fold and 4.7-fold), GPx (1.45-fold), and SOD (1.34-fold and 1.27-fold) enzyme activities, whereas total GST (46% and 57%) and its isoforms,and GST mu (57% and 72%), and GST theta (60% and 68%) activities were significantly decreased. Western-blot and qRT-PCR analysis showed that NQO1 and GPx protein and mRNA expressions were increased significantly (P < 0.0001), whereas GST mu and GST theta were significantly decreased (P < 0.0001).
authors.affiliation: a Middle East Technical University, Department of Biological Sciences and Joint Graduate Program in Biochemistry, Ankara, Turkey, and Selcuk University, Faculty of Sciences, Department of Biochemistry , Konya , Turkey.
authors.forename: Serdar
authors.initials: S
authors.lastname: Karakurt
authors.affiliation: b Pamukkale University, Faculty of Sciences, Department of Biology , Denizli , Turkey.
authors.forename: Asli
authors.initials: A
authors.lastname: Semiz
authors.affiliation: b Pamukkale University, Faculty of Sciences, Department of Biology , Denizli , Turkey.
authors.forename: Gurbet
authors.initials: G
authors.lastname: Celik
authors.affiliation: c Ankara University, Faculty of Pharmacy, Department of Pharmaceutical Botany , Ankara , Turkey.
authors.forename: Ayse Mine
authors.initials: AM
authors.lastname: Gencler-Ozkan
authors.affiliation: b Pamukkale University, Faculty of Sciences, Department of Biology , Denizli , Turkey.
authors.forename: Alaattin
authors.initials: A
authors.lastname: Sen
authors.affiliation: d Middle East Technical University, Department of Biological Sciences and Joint Graduate Program in Biochemistry , Ankara , Turkey.
authors.forename: Orhan
authors.initials: O
authors.lastname: Adali
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D004610:Ellagic Acid / Q000494:pharmacology*; D031666:Epilobium; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008297:Male; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000378:metabolism; D010946:Plants, Medicinal; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D004610:Ellagic Acid; D013482:Superoxide Dismutase; D016660:NAD(P)H Dehydrogenase (Quinone); C507703:NQO1 protein, rat