title: Bi-Allelic TCRα or β Recombination Enhances T Cell Development but Is Dispensable for Antigen Responses and Experimental Autoimmune Encephalomyelitis.
abstract: Dual TCRα-expressing T cells outnumber dual TCRβ-expressing cells by ~10:1. As a result, efforts to understand how dual TCR T cells impact immunity have focused on dual TCRα expression; dual TCRβ expression remains understudied. We recently demonstrated, however, that dual TCRβ expression accelerated disease in a TCR transgenic model of autoimmune arthritis through enhanced positive selection efficiency, indicating that dual TCRβ expression, though rare, can impact thymic selection. Here we generated mice hemizygous for TCRα, TCRβ, or both on the C57BL/6 background to investigate the impact bi-allelic TCR chain recombination has on T cell development, repertoire diversity, and autoimmunity. Lack of bi-allelic TCRα or TCRβ recombination reduced αβ thymocyte development efficiency, and the absence of bi-allelic TCRβ recombination promoted γδ T cell development. However, we observed no differences in the numbers of naïve and expanded antigen-specific T cells between TCRα+/-β+/- and wildtype mice, and TCR repertoire analysis revealed only subtle differences in Vβ gene usage. Finally, the absence of dual TCR T cells did not impact induced experimental autoimmune encephalomyelitis pathogenesis. Thus, despite more stringent allelic exclusion of TCRβ relative to TCRα, bi-allelic TCRβ expression can measurably impact thymocyte development and is necessary for maintaining normal αβ/γδ T cell proportions.
authors.affiliation: Departments of Pediatrics, University of Minnesota, Minneapolis, United States of America.|Center for Immunology, University of Minnesota, Minneapolis, United States of America.
authors.forename: Nathaniel J
authors.initials: NJ
authors.lastname: Schuldt
authors.affiliation: Departments of Pediatrics, University of Minnesota, Minneapolis, United States of America.|Center for Immunology, University of Minnesota, Minneapolis, United States of America.
authors.forename: Jennifer L
authors.initials: JL
authors.lastname: Auger
authors.affiliation: Departments of Pediatrics, University of Minnesota, Minneapolis, United States of America.|Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, United States of America.|Center for Immunology, University of Minnesota, Minneapolis, United States of America.
authors.forename: Kristin A
authors.initials: KA
authors.lastname: Hogquist
authors.affiliation: Departments of Pediatrics, University of Minnesota, Minneapolis, United States of America.|Center for Immunology, University of Minnesota, Minneapolis, United States of America.
authors.forename: Bryce A
authors.initials: BA
authors.lastname: Binstadt
mesh_terms: D000818:Animals; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000235:genetics / Q000276:immunology*; D005786:Gene Expression Regulation / Q000276:immunology*; D015332:Gene Rearrangement, alpha-Chain T-Cell Antigen Receptor / Q000276:immunology*; D015333:Gene Rearrangement, beta-Chain T-Cell Antigen Receptor / Q000276:immunology*; D051379:Mice; D018345:Mice, Knockout; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics / Q000276:immunology*; D013601:T-Lymphocytes / Q000382:microbiology*
chemical_list: D016693:Receptors, Antigen, T-Cell, alpha-beta