title: Time-course proteome analysis of developing extrafloral nectaries of Ricinus communis.
abstract: Floral and extrafloral nectaries are unique organs that secrete energy rich chemical components, but their contribution for nectar production is largely unknown. Here, we present the first comparative proteome dataset of four developmental stages of the extrafloral nectaries from castor plant (Ricinus communis), an important biofuel crop. Respectively, from stage I-IV, we identified 626, 613, 449 and 356 proteins, respectively, summing up 882 nonredundant proteins. Surprisingly, we identified two isoforms of the potent toxin ricin, indicating that ricin expression is not limited to seeds, but it may serve a general defense purpose for the castor plant. To date, this is the most complete dataset of proteins either from floral or extrafloral nectaries, thus contributing to lay the foundations for investigations on their ecological and evolutionary importance.
authors.affiliation: Department of Biochemistry and Molecular Biology, Federal University of Ceara, Fortaleza, Brazil.
authors.forename: Mohibullah
authors.initials: M
authors.lastname: Shah
authors.affiliation: Department of Biochemistry and Molecular Biology, Federal University of Ceara, Fortaleza, Brazil.
authors.forename: Fabiano M
authors.initials: FM
authors.lastname: Teixeira
authors.affiliation: Department of Biochemistry and Molecular Biology, Federal University of Ceara, Fortaleza, Brazil.
authors.forename: Emanoella L
authors.initials: EL
authors.lastname: Soares
authors.affiliation: Department of Biology, Federal University of Ceara, Fortaleza, Brazil.
authors.forename: Arlete A
authors.initials: AA
authors.lastname: Soares
authors.affiliation: Laboratory for Proteomics and Protein Engineering, Carlos Chagas Institute, Fiocruz, Paraná, Brazil.
authors.forename: Paulo C
authors.initials: PC
authors.lastname: Carvalho
authors.affiliation: Proteomic Unit, Institute of Chemistry, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Gilberto B
authors.initials: GB
authors.lastname: Domont
authors.affiliation: Department of Biochemistry, Biophysics, & Molecular Biology, Iowa State University, Ames, IA, USA.
authors.forename: Robert W
authors.initials: RW
authors.lastname: Thornburg
authors.affiliation: Proteomic Unit, Institute of Chemistry, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Fábio C S
authors.initials: FC
authors.lastname: Nogueira
authors.affiliation: Department of Biochemistry and Molecular Biology, Federal University of Ceara, Fortaleza, Brazil.
authors.forename: Francisco A P
authors.initials: FA
authors.lastname: Campos
mesh_terms: D010940:Plant Proteins / Q000032:analysis / Q000378:metabolism*; D020543:Proteome / Q000032:analysis / Q000378:metabolism; D040901:Proteomics; D012276:Ricin / Q000032:analysis / Q000378:metabolism; D012278:Ricinus / Q000254:growth & development* / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D020543:Proteome; D012276:Ricin
keywords: Defense proteins; Nectary proteomics; Oilseeds proteomics; Plant proteomics; Ricin; Ricinus communis