title: In-situ nanoelectrospray for high-throughput screening of enzymes and real-time monitoring of reactions.
abstract: The in-situ and high-throughput evaluation of enzymes and real-time monitoring of enzyme catalyzed reactions in liquid phase is quite significant in the catalysis industry. In-situ nanoelectrospray, the direct sampling and ionization method for mass spectrometry, has been applied for high-throughput evaluation of enzymes, as well as the on-line monitoring of reactions. Simply inserting a capillary into a liquid system with high-voltage applied, analytes in liquid reaction system can be directly ionized at the capillary tip with small volume consumption. With no sample pre-treatment or injection procedure, different analytes such as saccharides, amino acids, alkaloids, peptides and proteins can be rapidly and directly extracted from liquid phase and ionized at the capillary tip. Taking irreversible transesterification reaction of vinyl acetate and ethanol as an example, this technique has been used for the high-throughput evaluation of enzymes, fast optimizations, as well as real-time monitoring of reaction catalyzed by different enzymes. In addition, it is even softer than traditional electrospray ionization. The present method can also be used for the monitoring of other homogenous and heterogeneous reactions in liquid phases, which will show potentials in the catalysis industry.
authors.affiliation: Key Laboratory of Theoretical and Computational Photochemistry, College of Chemistry, Beijing Normal University, Beijing 100875, PR China.
authors.forename: Yuhan
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Key Laboratory of Theoretical and Computational Photochemistry, College of Chemistry, Beijing Normal University, Beijing 100875, PR China.
authors.forename: Feifei
authors.initials: F
authors.lastname: Han
authors.affiliation: Key Laboratory of Theoretical and Computational Photochemistry, College of Chemistry, Beijing Normal University, Beijing 100875, PR China.
authors.forename: Jin
authors.initials: J
authors.lastname: Ouyang
authors.affiliation: Key Laboratory of Theoretical and Computational Photochemistry, College of Chemistry, Beijing Normal University, Beijing 100875, PR China.
authors.forename: Yunling
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Theoretical and Computational Photochemistry, College of Chemistry, Beijing Normal University, Beijing 100875, PR China.
authors.forename: Juan
authors.initials: J
authors.lastname: Han
authors.affiliation: Key Laboratory of Theoretical and Computational Photochemistry, College of Chemistry, Beijing Normal University, Beijing 100875, PR China. Electronic address: nana@bnu.edu.cn.
authors.forename: Na
authors.initials: N
authors.lastname: Na
mesh_terms: D004798:Enzymes / Q000032:analysis*; D057166:High-Throughput Screening Assays / Q000379:methods*; D036103:Nanotechnology / Q000379:methods*
chemical_list: D004798:Enzymes
keywords: Catalytic reaction; Enzymes; High-throughput screening; In-situ nanoelectrospray; Real-time monitoring