title: Short-term repeated corticosterone administration enhances glutamatergic but not GABAergic transmission in the rat motor cortex.
abstract: It has been demonstrated that stress impairs performance of skilled reaching and walking tasks in rats due to the action of glucocorticoids involved in the stress response. Skilled reaching and walking are controlled by the primary motor cortex (M1); however, it is not known whether stress-related impairments in skilled motor tasks are related to functional and/or structural alterations within the M1. We studied the effects of single and repeated injections of corticosterone (twice daily for 7 days) on spontaneous excitatory and inhibitory postsynaptic currents (sEPSCs and sIPSCs) recorded from layer II/III pyramidal neurons in ex vivo slices of the M1, prepared 2 days after the last administration of the hormone. We also measured the density of dendritic spines on pyramidal cells and the protein levels of selected subunits of AMPA, NMDA, and GABAA receptors after repeated corticosterone administration. Repeatedly administered corticosterone induced an increase in the frequency but not in the amplitude of sEPSCs, while a single administration had no effect on the recorded excitatory currents. The frequency and amplitude of sIPSCs as well as the excitability of pyramidal cells were changed neither after single nor after repeated corticosterone administration. Treatment with corticosterone for 7 days did not modify the density of dendritic spines on pyramidal neurons. Corticosterone influenced neither the protein levels of GluA1, GluA2, GluN1, GluN2A, and GluN2B subunits of glutamate receptors nor those of α1, β2, and γ2 subunits of the GABAA receptor. The increase in sEPSCs frequency induced by repeated corticosterone administration faded out within 7 days. These data indicate that prolonged administration of exogenous corticosterone selectively and reversibly enhances glutamatergic, but not GABAergic transmission in the rat motor cortex. Our results suggest that corticosterone treatment results in an enhancement of spontaneous glutamate release from presynaptic terminals in the M1 and thereby uncovers a potential mechanism underlying stress-induced motor functions impairment.
authors.affiliation: Institute of Zoology, Jagiellonian University, Gronostajowa 9, 30-387, Krakow, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Kula
authors.affiliation: Institute of Zoology, Jagiellonian University, Gronostajowa 9, 30-387, Krakow, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Blasiak
authors.affiliation: Institute of Zoology, Jagiellonian University, Gronostajowa 9, 30-387, Krakow, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Czerw
authors.affiliation: Institute of Zoology, Jagiellonian University, Gronostajowa 9, 30-387, Krakow, Poland.
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Tylko
authors.affiliation: Department of Physiology, Institute of Pharmacology, Polish Academy of Sciences, Smetna 12, 31-343, Krakow, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Sowa
authors.affiliation: Institute of Zoology, Jagiellonian University, Gronostajowa 9, 30-387, Krakow, Poland. grzegorz.hess@uj.edu.pl.|Department of Physiology, Institute of Pharmacology, Polish Academy of Sciences, Smetna 12, 31-343, Krakow, Poland. grzegorz.hess@uj.edu.pl.
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Hess
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003345:Corticosterone / Q000008:administration & dosage / Q000494:pharmacology*; D019706:Excitatory Postsynaptic Potentials; D059330:GABAergic Neurons / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D018698:Glutamic Acid / Q000378:metabolism*; D053444:Inhibitory Postsynaptic Potentials; D008297:Male; D009044:Motor Cortex / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D017966:Pyramidal Cells / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D018091:Receptors, AMPA / Q000235:genetics / Q000378:metabolism; D011963:Receptors, GABA-A / Q000235:genetics / Q000378:metabolism; D016194:Receptors, N-Methyl-D-Aspartate / Q000235:genetics / Q000378:metabolism; D013569:Synapses / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
chemical_list: D018091:Receptors, AMPA; D011963:Receptors, GABA-A; D016194:Receptors, N-Methyl-D-Aspartate; D018698:Glutamic Acid; D005680:gamma-Aminobutyric Acid; D003345:Corticosterone
keywords: Brain slices; Model of stress; Neocortex