title: Characterization of a conditional interleukin-1 receptor 1 mouse mutant using the Cre/LoxP system.
abstract: IL-1 is a key cytokine known to drive chronic inflammation and to regulate many physiological, immunological, and neuroimmunological responses via actions on diverse cell types of the body. To determine the mechanisms of IL-1 actions as part of the inflammatory response in vivo, we generated a conditional IL-1 receptor 1 (IL-1R1) mouse mutant using the Cre/LoxP system (IL-1R1(fl/fl) ). In the mutant generated, exon 5, which encodes part of the extracellular-binding region of the receptor, is flanked by LoxP sites, thereby inactivating the two previously described functional IL-1R1 gene transcripts after Cre-mediated recombination. Using keratin 14-Cre driver mice, new IL-1R1 deficient (-/-) mice were subsequently generated, in which all signaling IL-1 receptor isoforms are deleted ubiquitously. Furthermore, using vav-iCre driver mice, we deleted IL-1 receptor isoforms in the hematopoietic system. In these mice, we show that both the IL-17 and IL-22 cytokine response is reduced, when mice are challenged by the helminth Trichuris muris. We are currently crossing IL-1R1(fl/fl) mice with different Cre-expressing mice in order to study mechanisms of acute and chronic inflammatory diseases.
authors.affiliation: Faculty of Life Sciences, University of Manchester, Manchester, UK.|Biochemistry Department, Faculty of Sciences, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia.
authors.forename: Wesam H
authors.initials: WH
authors.lastname: Abdulaal
authors.affiliation: Faculty of Life Sciences, University of Manchester, Manchester, UK.
authors.forename: Catherine R
authors.initials: CR
authors.lastname: Walker
authors.affiliation: Faculty of Life Sciences, University of Manchester, Manchester, UK.
authors.forename: Ryan
authors.initials: R
authors.lastname: Costello
authors.affiliation: Faculty of Life Sciences, University of Manchester, Manchester, UK.
authors.forename: Elena
authors.initials: E
authors.lastname: Redondo-Castro
authors.affiliation: Institute for Molecular Medicine, Medical University of Johannes Gutenberg-University of Mainz, Mainz, Germany.
authors.forename: Ilgiz A
authors.initials: IA
authors.lastname: Mufazalov
authors.affiliation: Faculty of Life Sciences, University of Manchester, Manchester, UK.
authors.forename: Athina
authors.initials: A
authors.lastname: Papaemmanouil
authors.affiliation: Faculty of Life Sciences, University of Manchester, Manchester, UK.
authors.forename: Nancy J
authors.initials: NJ
authors.lastname: Rothwell
authors.affiliation: Faculty of Life Sciences, University of Manchester, Manchester, UK.
authors.forename: Stuart M
authors.initials: SM
authors.lastname: Allan
authors.affiliation: Institute for Molecular Medicine, Medical University of Johannes Gutenberg-University of Mainz, Mainz, Germany.
authors.forename: Ari
authors.initials: A
authors.lastname: Waisman
authors.affiliation: Faculty of Life Sciences, University of Manchester, Manchester, UK.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Pinteaux
authors.affiliation: Faculty of Life Sciences, University of Manchester, Manchester, UK.
authors.forename: Werner
authors.initials: W
authors.lastname: Müller
mesh_terms: D000818:Animals; D007249:Inflammation / Q000276:immunology*; D020381:Interleukin-17 / Q000096:biosynthesis* / Q000276:immunology; D007378:Interleukins / Q000096:biosynthesis* / Q000276:immunology; D053547:Keratin-14 / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D053573:Receptors, Interleukin-1 Type I / Q000235:genetics* / Q000276:immunology; D014258:Trichuris / Q000276:immunology*; D000097043:Interleukin-22
chemical_list: D020381:Interleukin-17; D007378:Interleukins; D053547:Keratin-14; D053573:Receptors, Interleukin-1 Type I; D000097043:Interleukin-22; C583355:IL1R1 protein, mouse; C098680:Il17a protein, mouse; C507446:Krt14 protein, mouse
keywords: Cre/loxP; IL-17; Immune regulation; Infection; Trichuris muris