title: Determination of parthenolide in rat plasma by UPLC-MS/MS and its application to a pharmacokinetic study.
abstract: A rapid, sensitive and selective ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method was developed and validated for the determination and pharmacokinetic investigation of parthenolide in rat plasma. Sample preparation was accomplished through a simple one-step deproteinization procedure with 0.2mL of acetonitrile containing 30ng/mL of pirfenidone (IS), and to a 0.1mL plasma sample. Plasma samples were separated by UPLC on an Acquity UPLC BEH C18 column using a mobile phase consisting of acetonitrile-0.1% formic acid in water with gradient elution. The total run time was 3.0min and the elution of parthenolide was at 1.33min. The detection was performed on a triple quadrupole tandem mass spectrometer in the multiple reaction-monitoring (MRM) mode using the respective transitions m/z 249.2→231.1 for parthenolide and m/z 186.2→92.1 for pirfenidone (IS), respectively. The calibration curve was linear over the range of 2.0-500ng/mL with a lower limit of quantitation (LLOQ) of 2.0ng/mL. Mean recovery of parthenolide in plasma was in the range of 78.2-86.6%. Intra-day and inter-day precision were both <8.3%. This method was successfully applied in pharmacokinetic study after oral and intravenous administration of parthenolide in rats.
authors.affiliation: Luoyang Orthopedic Traumatological Hospital of Henan Province, Henan Province Hospital of Orthopedics, Luoyang 471002, China.
authors.forename: Ai-qin
authors.initials: AQ
authors.lastname: Zhao
authors.affiliation: Medical College of Henan University of Science and Technology, Luoyang 471003, China. Electronic address: zgyzjh1668@126.com.
authors.forename: Ji-hong
authors.initials: JH
authors.lastname: Zhao
authors.affiliation: Luoyang Orthopedic Traumatological Hospital of Henan Province, Henan Province Hospital of Orthopedics, Luoyang 471002, China.
authors.forename: Shu-qing
authors.initials: SQ
authors.lastname: Zhang
authors.affiliation: Medical College of Henan University of Science and Technology, Luoyang 471003, China.
authors.forename: Yong-yang
authors.initials: YY
authors.lastname: Pan
authors.affiliation: Medical College of Henan University of Science and Technology, Luoyang 471003, China.
authors.forename: Xu-lei
authors.initials: XL
authors.lastname: Huo
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002138:Calibration; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D004355:Drug Stability; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics*; D066298:In Vitro Techniques; D007275:Injections, Intravenous; D057230:Limit of Detection; D008297:Male; D017207:Rats, Sprague-Dawley; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012717:Sesquiterpenes / Q000008:administration & dosage / Q000097:blood*; D053719:Tandem Mass Spectrometry / Q000295:instrumentation / Q000379:methods*
chemical_list: D004365:Drugs, Chinese Herbal; D012717:Sesquiterpenes; C002669:parthenolide
keywords: Parthenolide; Pharmacokinetics; Rat plasma; UPLC-MS/MS