title: Molecular identification of Bigeyes (Perciformes, Priacanthidae) from Indian waters.
abstract: Thirty-five individuals of six priacanthid fish species were sampled from different localities along the coast of India covering the Arabian Sea and Bay of Bengal. The partial sequence of 16S rRNA and cytochrome oxidase subunit I (COI) genes were analyzed for species identification and phylogenetic relationship among the Indian priacanthids (Priacanthus hamrur, P. prolixus, P. blochii, P. sagittarius, Cookeolus japonicus, and Pristigenys refulgens). The intraspecies genetic distance ranged from 0.000 to 0.002, while distances varied from 0.008 to 0.157 interspecies based on 16S sequences. Using COI data analysis, the intraspecies genetic distance ranged from 0.000 to 0.005, while interspecies distances varied from 0.009 to 0.108. Several sequences labeled Priacanthus hamrur in GenBank are shown to be P. prolixus. We also observed cryptic speciation in Heteropriacanthus cruentatus. Partial sequences of 16S rRNA and COI genes provided phylogenetic information to distinguish thirteen species of priacanthids, indicating the usefulness of molecular markers in species identification.
authors.affiliation: a National Bureau of Fish Genetic Resources, Central Marine Fisheries Research Institute Campus , Kochi , Kerala , India.
authors.forename: Bineesh
authors.initials: B
authors.lastname: K K
authors.affiliation: b Central Marine Fisheries Research Institute , Kochi , Kerala , India.
authors.forename: Gopalakrishnan
authors.initials: G
authors.lastname: A
authors.affiliation: c National Bureau of Fish Genetic Resources , Dilkusha , Lucknow , India , and.
authors.forename: Jena
authors.initials: J
authors.lastname: J K
authors.affiliation: a National Bureau of Fish Genetic Resources, Central Marine Fisheries Research Institute Campus , Kochi , Kerala , India.
authors.forename: Basheer
authors.initials: B
authors.lastname: V S
authors.affiliation: a National Bureau of Fish Genetic Resources, Central Marine Fisheries Research Institute Campus , Kochi , Kerala , India.
authors.forename: Mohitha
authors.initials: M
authors.lastname: C
authors.affiliation: a National Bureau of Fish Genetic Resources, Central Marine Fisheries Research Institute Campus , Kochi , Kerala , India.
authors.forename: Vineesh
authors.initials: V
authors.lastname: N
authors.affiliation: d Nirmalagiri College , Kannur , Kerala , India.
authors.forename: Joselet
authors.initials: J
authors.lastname: M
authors.affiliation: b Central Marine Fisheries Research Institute , Kochi , Kerala , India.
authors.forename: N G K
authors.initials: NG
authors.lastname: Pillai
mesh_terms: D000818:Animals; D001482:Base Composition; D004272:DNA, Mitochondrial / Q000302:isolation & purification / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000378:metabolism; D054629:Genome, Mitochondrial; D007194:India; D010473:Perciformes / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000737:chemistry / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004272:DNA, Mitochondrial; D012336:RNA, Ribosomal, 16S; D003576:Electron Transport Complex IV
keywords: 16S rRNA; Bigeyes; COI; DNA barcoding; Indian waters