title: Evaluation of Bacillus spp. as dough starters for Adhirasam - A traditional rice based fermented food of Southern India.
abstract: Adhirasam is a cereal based, doughnut shaped, deep fried dessert consumed in the southern regions of India. The dough used to prepare adhirasam is fermented and contains rice flour and jaggery. The aim of the present study was to characterize the cultivable bacteria associated with this fermented dough and to identify a suitable starter culture for the production of quality adhirasam. In total, one hundred and seventy bacterial isolates were recovered from de Man Rogosa Sharp (MRS) agar, nutrient agar, lysogeny agar and tryptic soy agar media. Out of the 170 bacterial isolates, sixteen isolates were selected based on their ability to tolerate glucose and sucrose. All the bacterial isolates tolerated 15% glucose and 30% sucrose. Analyses of 16S rDNA gene sequences of the bacterial isolates showed that the dominant cultivable bacteria were members of the genus Bacillus. These strains were further used as starters and tested for their ability to ferment rice flour with jaggery to produce adhirasam dough. Organoleptic evaluation was carried out to choose the best starter strain. Adhirasam prepared from Bacillus subtilis isolates S4-P11, S2-G2-A1 and S1-G15, Bacillus tequilensis isolates S2-H16, S3-P9, S3-G10 and Bacillus siamensis isolate S2-G13 were highly acceptable to consumers. Adhirasam prepared using these starter cultures had superior product characteristics such as softness in texture, flavor and enhanced aroma and sweet taste.
authors.affiliation: Department of Food Science, Tamil Nadu Agricultural University, Tamil Nadu, India.
authors.forename: Anvar Hussain Noorul
authors.initials: AH
authors.lastname: Anisha
authors.affiliation: Department of Agricultural Microbiology, Tamil Nadu Agricultural University, Tamil Nadu, India.
authors.forename: Rangasamy
authors.initials: R
authors.lastname: Anandham
authors.affiliation: Korean Agricultural Culture Collection, National Academy of Agricultural Science, Republic of Korea.
authors.forename: Soon Woo
authors.initials: SW
authors.lastname: Kwon
authors.affiliation: Regional Research Station, Tamil Nadu Agricultural University, Tamil Nadu, India.
authors.forename: Pandiyan Indira
authors.initials: PI
authors.lastname: Gandhi
authors.affiliation: Department of Agricultural Microbiology, Tamil Nadu Agricultural University, Tamil Nadu, India.
authors.forename: Nellaiappan Olaganathan
authors.initials: NO
authors.lastname: Gopal
mesh_terms: D001407:Bacillus / Q000145:classification / Q000254:growth & development* / Q000302:isolation & purification / Q000378:metabolism*; D015373:Bacterial Typing Techniques; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005516:Food Microbiology; D006801:Humans; D007194:India; D012275:Oryza / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S