title: Prolonged and Continuous Measurement of Kidney Oxygenation in Conscious Rats.
abstract: A relative deficiency in kidney oxygenation, i.e., renal hypoxia, may contribute to the initiation and progression of acute and chronic kidney disease. A critical barrier to investigate this is the lack of methods allowing measurement of the partial pressure of oxygen in kidney tissue for long periods in vivo. We have developed, validated, and tested a novel telemetric method that can do this. Here we provide details on the calibration, implantation, implementation for data recording, and reuse of this telemetry-based technology for measurement of medullary tissue oxygen tension in conscious, unrestrained rats. This technique provides an important additional tool for investigating the impact of renal hypoxia in biology and pathophysiology.
authors.affiliation: School of Physiology and Pharmacology, University of Bristol, Medical Sciences Building, University Walk, Bristol, BS8 1TD, UK. m.koeners@bristol.ac.nz.|Department of Physiology, Auckland Bioengineering Institute, University of Auckland, Auckland, New Zealand. m.koeners@bristol.ac.nz.|Department of Nephrology, University Medical Centre Utrecht, Utrecht, The Netherlands. m.koeners@bristol.ac.nz.
authors.forename: Maarten P
authors.initials: MP
authors.lastname: Koeners
authors.affiliation: Department of Physiology, Monash University, Melbourne, VIC, Australia.
authors.forename: Connie P C
authors.initials: CPC
authors.lastname: Ow
authors.affiliation: Department of Physiology, Auckland Bioengineering Institute, University of Auckland, Auckland, New Zealand.|Millar Ltd, Auckland, New Zealand.
authors.forename: David M
authors.initials: DM
authors.lastname: Russell
authors.affiliation: Department of Physiology, Monash University, Melbourne, VIC, Australia.
authors.forename: Roger G
authors.initials: RG
authors.lastname: Evans
authors.affiliation: Department of Physiology, Auckland Bioengineering Institute, University of Auckland, Auckland, New Zealand.|Millar Ltd, Auckland, New Zealand.
authors.forename: Simon C
authors.initials: SC
authors.lastname: Malpas
mesh_terms: D000818:Animals; D007668:Kidney / Q000378:metabolism* / Q000503:physiopathology; D010101:Oxygen Consumption; D051381:Rats; D013686:Telemetry / Q000379:methods*
keywords: Kidney; Medulla; Telemetry; Tissue oxygen concentration