title: Does Dietary Deoxynivalenol Modulate the Acute Phase Reaction in Endotoxaemic Pigs?--Lessons from Clinical Signs, White Blood Cell Counts, and TNF-Alpha.
abstract: We studied the interaction between deoxynivalenol (DON)-feeding and a subsequent pre- and post-hepatic immune stimulus with the hypothesis that the liver differently mediates the acute phase reaction (APR) in pigs. Barrows (n = 44) were divided into a DON-(4.59 mg DON/kg feed) and a control-diet group, surgically equipped with permanent catheters pre- (V. portae hepatis) and post-hepatic (V. jugularis interna) and infused either with 0.9% NaCl or LPS (7.5 µg/kg BW). Thus, combination of diet (CON vs. DON) and infusion (CON vs. LPS, jugular vs. portal) created six groups: CON_CON(jug.)-CON(por.), CON_CON(jug.)-LPS(por.), CON_LPS(jug.)-CON(por.), DON_CON(jug.)-CON(por.), DON_CON(jug.)-LPS(por.), DON_LPS(jug.)-CON(por.). Blood samples were taken at -30, 15, 30, 45, 60, 75, 90, 120, 150, 180 min relative to infusion and analyzed for leukocytes and TNF-alpha. Concurrently, clinical signs were scored and body temperature measured during the same period. LPS as such induced a dramatic rise in TNF-alpha (p < 0.001), hyperthermia (p < 0.01), and severe leukopenia (p < 0.001). In CON-fed pigs, an earlier return to physiological base levels was observed for the clinical complex, starting at 120 min post infusionem (p < 0.05) and persisting until 180 min. DON_LPS(jug.)-CON(por.) resulted in a lower temperature rise (p = 0.08) compared to CON_LPS(jug.)-CON(por.). In conclusion, APR resulting from a post-hepatic immune stimulus was altered by chronic DON-feeding.
authors.affiliation: Institute of Animal Nutrition, Friedrich-Loeffler Institute (FLI), Federal Research Institute of Animal Health, Bundesallee 50, 38116 Braunschweig, Germany. tanja.tesch@fli.bund.de.
authors.forename: Tanja
authors.initials: T
authors.lastname: Tesch
authors.affiliation: Institute of Animal Nutrition, Friedrich-Loeffler Institute (FLI), Federal Research Institute of Animal Health, Bundesallee 50, 38116 Braunschweig, Germany. erik.bannert@fli.bund.de.
authors.forename: Erik
authors.initials: E
authors.lastname: Bannert
authors.affiliation: Institute of Animal Nutrition, Friedrich-Loeffler Institute (FLI), Federal Research Institute of Animal Health, Bundesallee 50, 38116 Braunschweig, Germany. jeannette.kluess@fli.bund.de.
authors.forename: Jeannette
authors.initials: J
authors.lastname: Kluess
authors.affiliation: Institute of Animal Nutrition, Friedrich-Loeffler Institute (FLI), Federal Research Institute of Animal Health, Bundesallee 50, 38116 Braunschweig, Germany. jana.frahm@fli.bund.de.
authors.forename: Jana
authors.initials: J
authors.lastname: Frahm
authors.affiliation: Institute of Animal Nutrition, Friedrich-Loeffler Institute (FLI), Federal Research Institute of Animal Health, Bundesallee 50, 38116 Braunschweig, Germany. Susanne.kersten@fli.bund.de.
authors.forename: Susanne
authors.initials: S
authors.lastname: Kersten
authors.affiliation: Institute for Physiology, University of Veterinary Medicine, Foundation, Bischofsholer Damm 15, 30173 Hannover, Germany. gerhard.breves@tiho-hannover.de.
authors.forename: Gerhard
authors.initials: G
authors.lastname: Breves
authors.affiliation: Institute of Anatomy, Otto von Guericke University Magdeburg, Leipziger Str. 44, 39120 Magdeburg, Germany. lydia.renner@med.ovgu.de.
authors.forename: Lydia
authors.initials: L
authors.lastname: Renner
authors.affiliation: Institute of Anatomy, Otto von Guericke University Magdeburg, Leipziger Str. 44, 39120 Magdeburg, Germany. stefan.kahlert@med.ovgu.de.
authors.forename: Stefan
authors.initials: S
authors.lastname: Kahlert
authors.affiliation: Institute of Anatomy, Otto von Guericke University Magdeburg, Leipziger Str. 44, 39120 Magdeburg, Germany. hermann-josef.rothkoetter@med.ovgu.de.
authors.forename: Hermann-Josef
authors.initials: HJ
authors.lastname: Rothkötter
authors.affiliation: Institute of Animal Nutrition, Friedrich-Loeffler Institute (FLI), Federal Research Institute of Animal Health, Bundesallee 50, 38116 Braunschweig, Germany. sven.daenicke@fli.bund.de.
authors.forename: Sven
authors.initials: S
authors.lastname: Dänicke
mesh_terms: D000210:Acute-Phase Reaction / Q000276:immunology*; D000818:Animals; D019446:Endotoxemia / Q000276:immunology*; D007958:Leukocyte Count; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects* / Q000276:immunology; D008297:Male; D013552:Swine; D014255:Trichothecenes / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology
chemical_list: D008070:Lipopolysaccharides; D014255:Trichothecenes; D014409:Tumor Necrosis Factor-alpha; C007262:deoxynivalenol
keywords: body core temperature; clinical symptoms; deoxynivalenol; leukocytes; lipopolysaccharide; liver; pig; tumor necrosis factor alpha