title: An adhesive conducting electrode material based on commercial mesoporous titanium dioxide as a support for Horseradish peroxidase for bioelectrochemical applications.
abstract: An adhesive conducting electrode material containing of graphite, biocompatible ion exchange polymer nafion(®) and commercial mesoporous TiO2 impregnated with horseradish peroxidase (HRP) is prepared and characterized by amperometric, UV-vis and N2 sorption methods. The factors influencing the performance of the resulting biosensor are studied in detail. The optimal electrode material consists of 45% graphite, 50% impregnated HRP-TiO2 and 5% nafion(®). The optimum conditions for H2O2 reduction are an applied potential of -0.3 V and 0.1 mM hydroquinone. Sensitivity and limit of detection in the optimum conditions are 1 A M(-1) cm(-2) and 1 µM correspondingly. The N2 sorption results show that the pore volume of TiO2 decreases sharply upon adsorption of HRP. The preparation process of the proposed enzyme electrode is straightforward and potentially can be used for preparation of carbon paste electrodes for bioelectrochemical detections.
authors.affiliation: AXES Research Group, Department of Chemistry, University of Antwerp, Groenenborgerlaan 171, 2020 Antwerp, Belgium; Laboratory of Adsorption and Catalysis (LADCA), Department of Chemistry, University of Antwerp, Universiteitsplein 1, B-2610 Wilrijk, Belgium.
authors.forename: Vanoushe
authors.initials: V
authors.lastname: Rahemi
authors.affiliation: AXES Research Group, Department of Chemistry, University of Antwerp, Groenenborgerlaan 171, 2020 Antwerp, Belgium.
authors.forename: Stanislav
authors.initials: S
authors.lastname: Trashin
authors.affiliation: Laboratory of Adsorption and Catalysis (LADCA), Department of Chemistry, University of Antwerp, Universiteitsplein 1, B-2610 Wilrijk, Belgium.
authors.forename: Vera
authors.initials: V
authors.lastname: Meynen
authors.affiliation: AXES Research Group, Department of Chemistry, University of Antwerp, Groenenborgerlaan 171, 2020 Antwerp, Belgium. Electronic address: karolien.dewael@uantwerpen.be.
authors.forename: Karolien
authors.initials: K
authors.lastname: De Wael
mesh_terms: D000269:Adhesives / Q000737:chemistry*; D004553:Electric Conductivity; D004563:Electrochemistry / Q000295:instrumentation*; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D005465:Fluorocarbon Polymers / Q000737:chemistry; D006108:Graphite / Q000737:chemistry; D006735:Horseradish Peroxidase / Q000737:chemistry* / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000737:chemistry*; D057230:Limit of Detection; D009584:Nitrogen / Q000737:chemistry; D016062:Porosity; D013997:Time Factors; D014025:Titanium / Q000737:chemistry*
chemical_list: D000269:Adhesives; D004800:Enzymes, Immobilized; D005465:Fluorocarbon Polymers; C009495:titanium dioxide; C040402:perfluorosulfonic acid; D006108:Graphite; D006861:Hydrogen Peroxide; D014025:Titanium; D006735:Horseradish Peroxidase; D009584:Nitrogen
keywords: Amperometry; Composite electrode; Hydrogen peroxide; Immobilized enzyme; Mesoporous titania