title: Ultrasensitive electrochemical immunosensor for SCCA detection based on ternary Pt/PdCu nanocube anchored on three-dimensional graphene framework for signal amplification.
abstract: In this study, a novel and ultrasensitive sandwich-type electrochemical immunosensor was designed for the quantitative detection of squamous cell carcinoma antigen (SCCA) based on the β-cyclodextrin functionalized graphene nanosheet (CD-GN) and the ternary hollow Pt/PdCu nanocube anchored on three-dimensional graphene framework (Pt/PdCu-3DGF). CD-GN exhibited high specific surface area and good dispersibility and stability in water, which were beneficial to fix captured antibodies (Ab1) through the supramolecular host-guest interaction between CD and Ab1. The abundant oxygen-containing functional groups on 3DGF provided binding sites for anchoring noble metal nanoparticles. Pt/PdCu-3DGF could capture detected antibodies via the interaction of Pd-NH2 and Pt-NH2. Furthermore, the ternary metal nanoparticles exhibited high electrocatalytic activity toward the reduction of hydrogen peroxide. Under optimal conditions, the fabricated immunosensor showed a sensitive response to SCCA with two linear ranges. The linear ranges are 0.0001-1 ng/mL and 1-30 ng/mL with a detection limit of 25 fg/mL. Additionally, the proposed immunosensor showed good reproducibility and stability.
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China. Electronic address: mahongmin2002@126.com.
authors.forename: Hongmin
authors.initials: H
authors.lastname: Ma
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Gao
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Wu
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Ren
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Yan
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors.forename: Xuehui
authors.initials: X
authors.lastname: Pang
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Wei
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry; D000951:Antigens, Neoplasm / Q000097:blood*; D015374:Biosensing Techniques / Q000379:methods; D003300:Copper / Q000737:chemistry; D055664:Electrochemical Techniques / Q000379:methods*; D006108:Graphite / Q000737:chemistry; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D008958:Models, Molecular; D010165:Palladium / Q000737:chemistry*; D010984:Platinum / Q000737:chemistry*; D015203:Reproducibility of Results; D015843:Serpins / Q000097:blood*; D047392:beta-Cyclodextrins / Q000737:chemistry
chemical_list: D055910:Antibodies, Immobilized; D000951:Antigens, Neoplasm; D015843:Serpins; D047392:beta-Cyclodextrins; C051072:squamous cell carcinoma-related antigen; D010984:Platinum; D010165:Palladium; D006108:Graphite; D003300:Copper; C031215:betadex
keywords: Host-guest interaction; Pt/PdCu nanocube; Sandwich-type electrochemical immunosensor; β-cyclodextrin functionalized graphene nanosheet