title: Chondrogenic effect of cell-based scaffold of self-assembling peptides/PLGA-PLL loading the hTGFβ3 plasmid DNA.
abstract: With the application of tissue engineering to tissue regeneration, additional new complexes have been made in response to the challenge of cartilage-injury repair. This study was performed to construct a rat precartilaginous stem cells-based scaffold of self-assembling peptides RADA16-I/PLGA-PLL (poly-L-lysine coated PLGA) as extracellular matrix loading the NLS-TAT as a peptide-based carrier for a plasmid DNA containing hTGFβ3. After composites were cultured for 1, 2, 3 and 4 weeks, respectively, the results showed that the levels of chondrogenic-related gene expression were higher in the experimental group with and hTGFβ3 gene by reverse transcription-polymerase chain reaction, and with higher histochemical and immunohistochemical expression. hTGFβ3 protein expression had increased at 4 weeks based on western blot analysis. The results of this study show that a complex may be a suitable scaffold for cartilage repair and offer a strategy for tissue regeneration through the use of tissue engineering.
authors.affiliation: Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, Hubei, China.
authors.forename: Qiyong
authors.initials: Q
authors.lastname: Pan
authors.affiliation: Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, Hubei, China.
authors.forename: Wenkai
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, Hubei, China.
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Yuan
authors.affiliation: Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, Hubei, China.
authors.forename: Yeltay
authors.initials: Y
authors.lastname: Rakhmanov
authors.affiliation: Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, Hubei, China.
authors.forename: Pengcheng
authors.initials: P
authors.lastname: Wang
authors.affiliation: Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, Hubei, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Lu
authors.affiliation: Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, Hubei, China.
authors.forename: Zekai
authors.initials: Z
authors.lastname: Mao
authors.affiliation: Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, Hubei, China.
authors.forename: Xiaobin
authors.initials: X
authors.lastname: Shang
authors.affiliation: Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, Hubei, China. hbyou@aliyun.com.
authors.forename: Hongbo
authors.initials: H
authors.lastname: You
mesh_terms: D000818:Animals; D002356:Cartilage / Q000254:growth & development* / Q000502:physiology; D002478:Cells, Cultured; D004247:DNA / Q000008:administration & dosage*; D006801:Humans; D019344:Lactic Acid; D010957:Plasmids; D011091:Polyesters; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers; D051381:Rats; D012038:Regeneration; D023822:Tissue Engineering; D054457:Tissue Scaffolds; D053782:Transforming Growth Factor beta3 / Q000235:genetics*
chemical_list: D011091:Polyesters; D011108:Polymers; C509044:TGFB3 protein, human; D053782:Transforming Growth Factor beta3; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; C033616:poly(lactide); D004247:DNA