title: Cell adhesion molecule control of planar spindle orientation.
abstract: Polarized epithelial cells align the mitotic spindle in the plane of the sheet to maintain tissue integrity and to prevent malignant transformation. The orientation of the spindle apparatus is regulated by the immobilization of the astral microtubules at the lateral cortex and depends on the precise localization of the dynein-dynactin motor protein complex which captures microtubule plus ends and generates pulling forces towards the centrosomes. Recent developments indicate that signals derived from intercellular junctions are required for the stable interaction of the dynein-dynactin complex with the cortex. Here, we review the molecular mechanisms that regulate planar spindle orientation in polarized epithelial cells and we illustrate how different cell adhesion molecules through distinct and non-overlapping mechanisms instruct the cells to align the mitotic spindle in the plane of the sheet.
authors.affiliation: Institute-Associated Research Group "Cell Adhesion and Cell Polarity", Institute of Medical Biochemistry, ZMBE, University of Münster, Von-Esmarch-Str. 56, 48149, Muenster, Germany.
authors.forename: Hüseyin
authors.initials: H
authors.lastname: Tuncay
authors.affiliation: Institute-Associated Research Group "Cell Adhesion and Cell Polarity", Institute of Medical Biochemistry, ZMBE, University of Münster, Von-Esmarch-Str. 56, 48149, Muenster, Germany. ebnetk@uni-muenster.de.|Interdisciplinary Clinical Research Center (IZKF), University of Münster, 48419, Muenster, Germany. ebnetk@uni-muenster.de.
authors.forename: Klaus
authors.identifier: http://orcid.org/0000-0002-0417-7888
authors.initials: K
authors.lastname: Ebnet
mesh_terms: D000818:Animals; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D016764:Cell Polarity; D004847:Epithelial Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D008941:Spindle Apparatus / Q000378:metabolism*
chemical_list: D015815:Cell Adhesion Molecules
keywords: Cadherin; Cdc42; Cortical actin; JAM-A; Junctional adhesion molecules; LGN; MDCK; PtdIns(3,4,5)P3