title: Mitochondrial Cytochrome Oxidase I Gene Sequence Analysis of Aedes Albopictus in Malaysia.
abstract: A study was conducted to establish polymorphic variation of the mitochondrial DNA encoding the cytochrome oxidase subunit 1 (CO1) gene in Aedes albopictus isolated from 2 hot spot dengue-infested areas in the Subang Jaya District, Malaysia. A phylogenetic analysis was performed with the use of sequences obtained from USJ6 and Taman Subang Mas (TSM). Comparison of the local CO1 sequences with a laboratory strain (USM), alongside reference strains derived from the GenBank database revealed low genetic variation in terms of nucleotide differences and haplotype diversity. Four methods were used to construct a phylogenetic tree and illustrate the genetic relationship of the 37 Ae. albopictus populations based on the CO1 sequences, namely neighbor-joining (NJ), maximum parsimony (MP), maximum likelihood (ML), and Bayesian method, which revealed a distinct relationship between isolates from USJ6 and TSM. Our findings provide new information regarding the genetic diversity among morphologically similar Ae. albopictus, which has not been reported to date.
authors.affiliation: 1  Faculty of Health Sciences, Universiti Teknologi MARA, 42300 Puncak Alam, Selangor, Malaysia.
authors.forename: Nurul-Ain
authors.initials: NA
authors.lastname: Ismail
authors.affiliation: 1  Faculty of Health Sciences, Universiti Teknologi MARA, 42300 Puncak Alam, Selangor, Malaysia.
authors.forename: Nazri Che
authors.initials: NC
authors.lastname: Dom
authors.affiliation: 1  Faculty of Health Sciences, Universiti Teknologi MARA, 42300 Puncak Alam, Selangor, Malaysia.
authors.forename: Rodziah
authors.initials: R
authors.lastname: Ismail
authors.affiliation: 2  School of Biological Sciences, Universiti Sains Malaysia, 11800 Pulau Pinang, Malaysia.
authors.forename: Abu Hassan
authors.initials: AH
authors.lastname: Ahmad
authors.affiliation: 1  Faculty of Health Sciences, Universiti Teknologi MARA, 42300 Puncak Alam, Selangor, Malaysia.
authors.forename: Afiq
authors.initials: A
authors.lastname: Zaki
authors.affiliation: 1  Faculty of Health Sciences, Universiti Teknologi MARA, 42300 Puncak Alam, Selangor, Malaysia.
authors.forename: Siti Nazrina
authors.initials: SN
authors.lastname: Camalxaman
mesh_terms: D000330:Aedes / Q000201:enzymology* / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D003576:Electron Transport Complex IV / Q000235:genetics / Q000378:metabolism; D005260:Female; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism; D007303:Insect Vectors / Q000235:genetics* / Q000378:metabolism; D008296:Malaysia; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D016415:Sequence Alignment
chemical_list: D019476:Insect Proteins; D024101:Mitochondrial Proteins; D003576:Electron Transport Complex IV
keywords: Aedes albopictus; haplotype; mitochondrial DNA cytochrome oxidase 1; polymorphism; sequence variation