title: Independent Emergence of the Plasmodium falciparum Kelch Propeller Domain Mutant Allele C580Y in Guyana.
abstract: Suspected artemisinin resistance in Plasmodium falciparum can be explored by examining polymorphisms in the Kelch (PfK13) propeller domain. Sequencing of PfK13 and other gene resistance markers was performed on 98 samples from Guyana. Five of these samples carried the C580Y allele in the PfK13 propeller domain, with flanking microsatellite profiles different from those observed in Southeast Asia. These molecular data demonstrate independent emergence of the C580Y K13 mutant allele in Guyana, where resistance alleles to previously used drugs are fixed. Therefore, in Guyana and neighboring countries, continued molecular surveillance and periodic assessment of the therapeutic efficacy of artemisinin-based combination therapy are warranted.
authors.affiliation: Malaria Branch, Division of Parasitic Diseases and Malaria, Center for Global Health, Centers for Disease Control and Prevention, Atlanta.
authors.forename: Stella M
authors.initials: SM
authors.lastname: Chenet
authors.affiliation: Malaria Branch, Division of Parasitic Diseases and Malaria, Center for Global Health, Centers for Disease Control and Prevention, Atlanta Atlanta Research and Education Foundation, Georgia.
authors.forename: Sheila
authors.initials: S
authors.lastname: Akinyi Okoth
authors.affiliation: Malaria Branch, Division of Parasitic Diseases and Malaria, Center for Global Health, Centers for Disease Control and Prevention, Atlanta.
authors.forename: Curtis S
authors.initials: CS
authors.lastname: Huber
authors.affiliation: Guyana Ministry of Health.
authors.forename: Javin
authors.initials: J
authors.lastname: Chandrabose
authors.affiliation: Malaria Branch, Division of Parasitic Diseases and Malaria, Center for Global Health, Centers for Disease Control and Prevention, Atlanta.
authors.forename: Naomi W
authors.initials: NW
authors.lastname: Lucchi
authors.affiliation: Malaria Branch, Division of Parasitic Diseases and Malaria, Center for Global Health, Centers for Disease Control and Prevention, Atlanta Atlanta Research and Education Foundation, Georgia.
authors.forename: Eldin
authors.initials: E
authors.lastname: Talundzic
authors.affiliation: Guyana Ministry of Health.
authors.forename: Karanchand
authors.initials: K
authors.lastname: Krishnalall
authors.affiliation: Pan American Health Organization-Guyana.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Ceron
authors.affiliation: Laboratoire de Parasitologie, World Health Organization Collaborating Center for Surveillance of Antimalarial Drug Resistance, NRC for Malaria, Institut Pasteur de la Guyane, Cayenne, French Guiana.
authors.forename: Lise
authors.initials: L
authors.lastname: Musset
authors.affiliation: Malaria Branch, Division of Parasitic Diseases and Malaria, Center for Global Health, Centers for Disease Control and Prevention, Atlanta.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Macedo de Oliveira
authors.affiliation: President's Malaria Initiative, US Agency for International Development, Washington D.C.
authors.forename: Meera
authors.initials: M
authors.lastname: Venkatesan
authors.affiliation: National Malaria Program, Georgetown, Guyana.
authors.forename: Reyaud
authors.initials: R
authors.lastname: Rahman
authors.affiliation: Malaria Branch, Division of Parasitic Diseases and Malaria, Center for Global Health, Centers for Disease Control and Prevention, Atlanta.
authors.forename: John W
authors.initials: JW
authors.lastname: Barnwell
authors.affiliation: Malaria Branch, Division of Parasitic Diseases and Malaria, Center for Global Health, Centers for Disease Control and Prevention, Atlanta.
authors.forename: Venkatachalam
authors.initials: V
authors.lastname: Udhayakumar
mesh_terms: D000962:Antimalarials / Q000494:pharmacology; D037621:Artemisinins / Q000494:pharmacology; D016054:DNA, Protozoan / Q000032:analysis / Q000235:genetics; D004351:Drug Resistance / Q000235:genetics*; D004359:Drug Therapy, Combination; D006172:Guyana / Q000453:epidemiology; D006801:Humans; D016778:Malaria, Falciparum / Q000453:epidemiology / Q000469:parasitology*; D058889:Molecular Typing; D009154:Mutation / Q000235:genetics; D010963:Plasmodium falciparum / Q000187:drug effects* / Q000235:genetics*
chemical_list: D000962:Antimalarials; D037621:Artemisinins; D016054:DNA, Protozoan; C031327:artemisinin
keywords: ACT; Guyana; K13; Plasmodium falciparum; South America; artemisinin resistance; malaria