title: Determination of Histamine by High-Performance Liquid Chromatography After Precolumn Derivatization with o-Phthalaldehyde-Sulfite.
abstract: A fast and sensitive method was developed for in vivo determination of histamine in the brain microdialysate by reverse ion pair chromatography with electrochemical detection. The microdialysates were derivatized with o-phthalaldehyde and sodium sulfite, and separation was achieved using isocratic elution within 10 min. The separation was performed in an Agilent Eclipse Plus C18 column (3.0 × 150 mm, particle size 3.5 μm), and the mobile phase consisted of 100 mM monosodium phosphate (pH 6.0), 500 mg L(-1) OSA and 20% methanol (v/v). The linearity (R(2)) was found to be >0.999, with a range from 2 to 50 nM and excellent repeatability (relative standard deviation, 2.29-6.04%), and the limit of detection was 0.4 nM. This method was successfully applied to analyze the extracellular concentration of histamine in the hypothalamus of rats, with probe recovery calculated in vivo.
authors.affiliation: Research Center for Medicine and Biology, Zunyi Medical University, Zunyi, Guizhou, 563003, P. R. China Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan, Hubei 430071, P. R. China.
authors.forename: Rongxiang
authors.initials: R
authors.lastname: Chen
authors.affiliation: Department of Chemistry and Life Science, Hubei University of Education, Wuhan, Hubei 430205, P. R. China.
authors.forename: Yinghua
authors.initials: Y
authors.lastname: Deng
authors.affiliation: Department of Chemistry and Life Science, Hubei University of Education, Wuhan, Hubei 430205, P. R. China.
authors.forename: Liu
authors.initials: L
authors.lastname: Yang
authors.affiliation: Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan, Hubei 430071, P. R. China jie.wang@wipm.ac.cn.
authors.forename: Jie
authors.initials: J
authors.lastname: Wang
authors.affiliation: Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan, Hubei 430071, P. R. China Wuhan National Laboratory for Optoelectronics, Wuhan, Hubei 430074, P. R. China.
authors.forename: Fuqiang
authors.initials: F
authors.lastname: Xu
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006632:Histamine / Q000378:metabolism*; D057230:Limit of Detection; D008297:Male; D017551:Microdialysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D013447:Sulfites / Q000737:chemistry*; D009764:o-Phthalaldehyde / Q000737:chemistry*
chemical_list: D013447:Sulfites; D009764:o-Phthalaldehyde; D006632:Histamine