title: Tolerogenic dendritic cells show gene expression profiles that are different from those of immunogenic dendritic cells in DBA/1 mice.
abstract: Tolerogenic dendritic cells (tDCs) play an important role in inducing peripheral tolerance; however, few tDC-specific markers have been identified. The aims of this study were to examine whether tDCs show a different gene expression profile from that of immunogenic DCs and identify specific gene markers of each cell type, in DBA/1 mice. tDCs were generated by treating immature DCs (imDCs) with TNF-α and type II collagen. The gene expression profiles of mature (m)DCs and tDCs were then investigated by microarray analysis and candidate markers were validated by quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR). Supervised selection identified 75 gene signatures, 63 of which were consistently upregulated in mDCs and 12 of which were upregulated only in tDCs. Additionally, 10 genes were overexpressed or equally expressed in both tDCs and mDCs. Scin (tDC-specific genes) and Orm1, Pdlim4 and Enpp2 (mDC-specific genes) were validated by real-time qRT-PCR. Taken together, these results clearly show that tDCs and mDCs can be identified according to their expression of specific gene markers.
authors.affiliation: a Department of Biotechnology , CHA University, Gyeonggi-do , Republic of Korea .
authors.forename: Eun Gae
authors.initials: EG
authors.lastname: Lee
authors.affiliation: a Department of Biotechnology , CHA University, Gyeonggi-do , Republic of Korea .
authors.forename: Nam-Chul
authors.initials: NC
authors.lastname: Jung
authors.affiliation: a Department of Biotechnology , CHA University, Gyeonggi-do , Republic of Korea .
authors.forename: Jun-Ho
authors.initials: JH
authors.lastname: Lee
authors.affiliation: b Department of Radiation Cancer Sciences and.
authors.forename: Jie-Young
authors.initials: JY
authors.lastname: Song
authors.affiliation: c Department of Obstetrics & Gynecology , Korea Cancer Center Hospital, Korea Institute of Radiological and Medical Sciences , Seoul , Republic of Korea .
authors.forename: Sang-Young
authors.initials: SY
authors.lastname: Ryu
authors.affiliation: d Department of Animal Biotechnology , Konkuk University , Seoul , Republic of Korea .
authors.forename: Han Geuk
authors.initials: HG
authors.lastname: Seo
authors.affiliation: e Department of Food Science and Biotechnology , College of Animal Bioscience and Technology, Konkuk University , Seoul , Republic of Korea .
authors.forename: Sung Gu
authors.initials: SG
authors.lastname: Han
authors.affiliation: f Department of Science Education , Jeju National University, Jeju Special Self-Governing Province , Republic of Korea .
authors.forename: Keun Jae
authors.initials: KJ
authors.lastname: Ahn
authors.affiliation: g Division of MR Research , Korea Basic Science Institute , Cheongwon , Republic of Korea , and.
authors.forename: Kwan Soo
authors.initials: KS
authors.lastname: Hong
authors.affiliation: h Division of Rheumatology , Bundang CHA Medical Center , Gyeonggi-do , Republic of Korea.
authors.forename: Jinjung
authors.initials: J
authors.lastname: Choi
authors.affiliation: a Department of Biotechnology , CHA University, Gyeonggi-do , Republic of Korea .
authors.forename: Dae-Seog
authors.initials: DS
authors.lastname: Lim
mesh_terms: D000818:Animals; D015415:Biomarkers; D002478:Cells, Cultured; D016000:Cluster Analysis; D018920:Coculture Techniques; D019295:Computational Biology / Q000379:methods; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D063990:Gene Ontology; D007108:Immune Tolerance / Q000235:genetics*; D016130:Immunophenotyping; D051379:Mice; D008811:Mice, Inbred DBA; D015203:Reproducibility of Results; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D059467:Transcriptome
chemical_list: D015415:Biomarkers
keywords: Microarray; specific marker; tolerogenic dendritic cells