title: Sequence analysis of three canine adipokine genes revealed an association between TNF polymorphisms and obesity in Labrador dogs.
abstract: Obesity is an emerging health problem in purebred dogs. Due to their crucial role in energy homeostasis control, genes encoding adipokines are considered candidate genes, and their variants may be associated with predisposition to obesity. Searching for polymorphism was carried out in three adipokine genes (TNF, RETN and IL6). The study was performed on 260 dogs, including lean (n = 109), overweight (n = 88) and obese (n = 63) dogs. The largest cohort was represented by Labrador Retrievers (n = 136). Altogether, 24 novel polymorphisms were identified: 12 in TNF (including one missense SNP), eight in RETN (including one missense SNP) and four in IL6. Distributions of five common SNPs (two in TNF, two in RETN and one in IL6) were further analyzed with regard to body condition score. Two SNPs in the non-coding parts of TNF (c.-40A>C and c.233+14G>A) were associated with obesity in Labrador dogs. The obtained results showed that the studied adipokine genes are highly polymorphic and two polymorphisms in the TNF gene may be considered as markers predisposing Labrador dogs to obesity.
authors.affiliation: Department of Genetics and Animal Breeding, Poznan University of Life Sciences, Poznan, 60-637, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Mankowska
authors.affiliation: Department of Genetics and Animal Breeding, Poznan University of Life Sciences, Poznan, 60-637, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Stachowiak
authors.affiliation: Department of Genetics and Animal Breeding, Poznan University of Life Sciences, Poznan, 60-637, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Graczyk
authors.affiliation: Department of Genetics and Animal Breeding, Poznan University of Life Sciences, Poznan, 60-637, Poland.
authors.forename: P
authors.initials: P
authors.lastname: Ciazynska
authors.affiliation: Poznan University of Life Sciences, University Centre for Veterinary Medicine, Poznan, 60-637, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Gogulski
authors.affiliation: Department of Reproduction and Clinic of Farm Animals, Wroclaw University of Environmental and Life Sciences, Wroclaw, 50-366, Poland.
authors.forename: W
authors.initials: W
authors.lastname: Nizanski
authors.affiliation: Department of Genetics and Animal Breeding, Poznan University of Life Sciences, Poznan, 60-637, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Switonski
mesh_terms: D054392:Adipokines / Q000235:genetics*; D000818:Animals; D001947:Breeding; D004285:Dogs / Q000235:genetics*; D005838:Genotype; D006239:Haplotypes; D015850:Interleukin-6 / Q000235:genetics; D009765:Obesity / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D052243:Resistin / Q000235:genetics; D017422:Sequence Analysis, DNA; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
chemical_list: D054392:Adipokines; D015850:Interleukin-6; D052243:Resistin; D014409:Tumor Necrosis Factor-alpha
keywords: IL6; RETN; TNF; adiposity; cytokine; interleukin 6; resistin; tumor necrosis factor