title: New sorbent materials for selective extraction of cocaine and benzoylecgonine from human urine samples.
abstract: An increase in cocaine consumption has been observed in Europe during the last decade. Benzoylecgonine, as a main urinary metabolite of cocaine in human, is so far the most reliable marker of cocaine consumption. Determination of cocaine and its metabolite in complex biological samples as urine or blood, requires efficient and selective sample pretreatment. In this preliminary study, the newly synthesized sorbent materials were proposed for selective extraction of cocaine and benzoylecgonine from urine samples. Application of these sorbent media allowed to determine cocaine and benzoylecgonine in urine samples at the concentration level of 100ng/ml with good recovery values as 81.7%±6.6 and 73.8%±4.2, respectively. The newly synthesized materials provided efficient, inexpensive and selective extraction of both cocaine and benzoylecgonine from urine samples, which can consequently lead to an increase of the sensitivity of the current available screening diagnostic tests.
authors.affiliation: Department of Biophamaceutics and Pharmacodynamics, Medical University of Gdańsk, Hallera 107, 80-416 Gdańsk, Poland.
authors.forename: Renata
authors.initials: R
authors.lastname: Bujak
authors.affiliation: Department of Environmental Chemistry & Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, 7 Gagarin St., 87-100 Toruń, Poland.
authors.forename: Renata
authors.initials: R
authors.lastname: Gadzała-Kopciuch
authors.affiliation: Department of Organic Chemistry, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toruń, dr A. Jurasza, 85-089 Bydgoszcz, Poland.
authors.forename: Alicja
authors.initials: A
authors.lastname: Nowaczyk
authors.affiliation: Department of Biophamaceutics and Pharmacodynamics, Medical University of Gdańsk, Hallera 107, 80-416 Gdańsk, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Raczak-Gutknecht
authors.affiliation: Department of Biophamaceutics and Pharmacodynamics, Medical University of Gdańsk, Hallera 107, 80-416 Gdańsk, Poland.
authors.forename: Marta
authors.initials: M
authors.lastname: Kordalewska
authors.affiliation: Department of Biophamaceutics and Pharmacodynamics, Medical University of Gdańsk, Hallera 107, 80-416 Gdańsk, Poland.
authors.forename: Wiktoria
authors.initials: W
authors.lastname: Struck-Lewicka
authors.affiliation: Department of Biophamaceutics and Pharmacodynamics, Medical University of Gdańsk, Hallera 107, 80-416 Gdańsk, Poland.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Waszczuk-Jankowska
authors.affiliation: Department of Forensic Medicine, Medical University of Gdańsk, Dębowa 23, 80-204 Gdańsk, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Tomczak
authors.affiliation: Department of Forensic Medicine, Medical University of Gdańsk, Dębowa 23, 80-204 Gdańsk, Poland.
authors.forename: Michał
authors.initials: M
authors.lastname: Kaliszan
authors.affiliation: Department of Environmental Chemistry & Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, 7 Gagarin St., 87-100 Toruń, Poland.
authors.forename: Bogusław
authors.initials: B
authors.lastname: Buszewski
authors.affiliation: Department of Biophamaceutics and Pharmacodynamics, Medical University of Gdańsk, Hallera 107, 80-416 Gdańsk, Poland. Electronic address: markusz@gumed.edu.pl.
authors.forename: Michał J
authors.initials: MJ
authors.lastname: Markuszewski
mesh_terms: D003042:Cocaine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000652:urine*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D011108:Polymers / Q000737:chemistry*
chemical_list: D011108:Polymers; C005618:benzoylecgonine; D003042:Cocaine
keywords: Benzoylecgonine; Cocaine; Forensic analysis; Human urine; New sorbent media