title: Streptobacillus ratti sp. nov., isolated from a black rat (Rattus rattus).
abstract: An indole-, oxidase- and catalase-negative, non-motile bacterium, strain OGS16T, was isolated from an oral swab of a feral black rat (Rattus rattus) in 2007 in Japan. It stained Gram-negative and had pleomorphic, rod-shaped, non-spore-forming cells. Based on 16S rRNA gene sequence analyses, strain OGS16T was assigned to the genus Streptobacillus, with 16S rRNA gene sequence similarities of 99.3, 99.0, 98.6 and 95.5% to the type strains of Streptobacillus moniliformis, Streptobacillus notomytis, Streptobacillus felis and Streptobacillus hongkongensis, respectively. Strain OGS16T could also be differentiated clearly from other species of the genus Streptobacillus by rpoB, groEL and recA nucleotide and deduced amino acid sequence analysis. DNA-DNA relatedness as obtained by average nucleotide identity was 89.10% between strain OGS16T and Streptobacillus moniliformis DSM 12112T. Chemotaxonomic and physiological data for strain OGS16T were congruent with results for other closely related members of the family Leptotrichiaceae, represented by highly similar enzyme profiles and fatty acid patterns. MALDI-TOF MS analysis also proved suitable in discriminating strain OGS16T unequivocally from all currently described taxa of the genus Streptobacillus. On the basis of these data, we propose the novel species Streptobacillus ratti sp. nov., with the type strain OGS16T (=JCM 31098T=DSM 101843T). The G+C content of the DNA of the type strain is 25.9 mol% and the genome size is 1.50 Mbp.
authors.affiliation: Landesbetrieb Hessisches Landeslabor, D-35392 Giessen, Germany.
authors.forename: Tobias
authors.initials: T
authors.lastname: Eisenberg
authors.affiliation: Department of Veterinary Science, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.
authors.forename: Koichi
authors.initials: K
authors.lastname: Imaoka
authors.affiliation: Department of Veterinary Science, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.
authors.forename: Masanobu
authors.initials: M
authors.lastname: Kimura
authors.affiliation: Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany.
authors.forename: Stefanie P
authors.initials: SP
authors.lastname: Glaeser
authors.affiliation: Institut für Hygiene und Infektionskrankheiten der Tiere, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany.
authors.forename: Christa
authors.initials: C
authors.lastname: Ewers
authors.affiliation: Robert Koch-Institut, D-13353 Berlin, Germany.
authors.forename: Torsten
authors.initials: T
authors.lastname: Semmler
authors.affiliation: Chemisches und Veterinäruntersuchungsamt Stuttgart, D-70736 Fellbach, Germany.
authors.forename: Jörg
authors.initials: J
authors.lastname: Rau
authors.affiliation: Deutsches Krebsforschungszentrum, D-69120 Heidelberg, Germany.
authors.forename: Werner
authors.initials: W
authors.lastname: Nicklas
authors.affiliation: Ikari Corporation, Chiba 260-0844, Japan.
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Tanikawa
authors.affiliation: Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Kämpfer
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D005798:Genes, Bacterial; D007564:Japan; D008969:Molecular Sequence Data; D009055:Mouth / Q000382:microbiology*; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D051381:Rats / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013288:Streptobacillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S