title: Mitophagy in APPsw/PS1dE9 transgenic mice and APPsw stably expressing in HEK293 cells.
abstract: OBJECTIVE
  To observe mitophagy in APPsw/PS1dE9 transgenic mice and APPsw stably expressing HEK293 cell.
  
  
  METHODS
  The APPsw/PS1dE9 transgenic mice and 20E2 cell (HEK293 cell with APPsw) was used to be the model of Alzheimer's disease. We dynamically observed the behavior, mitochondrial structure and mitophagy in brain of APP/PS1 transgenic mice in different age groups. Mitochondrial structure and mitophagy of HEK293 and 20E2 cell in vitro were also recorded.
  
  
  RESULTS
  The mitochondrion was changed significantly in APP/PS1 transgenic mice and 20E2 cell. LC3 protein, associated with autophagy, was up-regulated. Mitophagy related protein PINK1 and PARKIN were up-regulated.
  
  
  CONCLUSIONS
  Mitophagy disorder may be associated with the pathogenesis of Alzheimer's Disease through PINK1/Parkin pathway.
authors.affiliation: Department of Neural Medicine, Second Hospital of Shandong University, Jinan, China.  bjz@sdu.edu.cn.
authors.forename: X
authors.initials: X
authors.lastname: Wang
authors.forename: X-L
authors.initials: XL
authors.lastname: Zhao
authors.forename: L-L
authors.initials: LL
authors.lastname: Xu
authors.forename: C-F
authors.initials: CF
authors.lastname: Wang
authors.forename: L-F
authors.initials: LF
authors.lastname: Wei
authors.forename: Z
authors.initials: Z
authors.lastname: Liu
authors.forename: H
authors.initials: H
authors.lastname: Yang
authors.forename: P
authors.initials: P
authors.lastname: Wang
authors.forename: Z-H
authors.initials: ZH
authors.lastname: Xie
authors.forename: J-Z
authors.initials: JZ
authors.lastname: Bi
mesh_terms: D000544:Alzheimer Disease / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D016564:Amyloid beta-Protein Precursor / Q000235:genetics*; D000818:Animals; D001921:Brain / Q000378:metabolism / Q000473:pathology; D002478:Cells, Cultured; D004195:Disease Models, Animal; D057809:HEK293 Cells; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D008928:Mitochondria / Q000378:metabolism; D063306:Mitophagy / Q000235:genetics*; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*; D044767:Ubiquitin-Protein Ligases / Q000235:genetics / Q000378:metabolism; D000099202:PTEN-Induced Putative Kinase
chemical_list: D016564:Amyloid beta-Protein Precursor; D011494:Protein Kinases; D044767:Ubiquitin-Protein Ligases; D000099202:PTEN-Induced Putative Kinase; C111567:parkin protein