title: Comparing genetic variants detected in the 1000 genomes project with SNPs determined by the International HapMap Consortium.
abstract: Single-nucleotide polymorphisms (SNPs) determined based on SNP arrays from the international HapMap consortium (HapMap) and the genetic variants detected in the 1000 genomes project (1KGP) can serve as two references for genomewide association studies (GWAS). We conducted comparative analyses to provide a means for assessing concerns regarding SNP array-based GWAS findings as well as for realistically bounding expectations for next generation sequencing (NGS)-based GWAS. We calculated and compared base composition, transitions to transversions ratio, minor allele frequency and heterozygous rate for SNPs from HapMap and 1KGP for the 622 common individuals. We analysed the genotype discordance between HapMap and 1KGP to assess consistency in the SNPs from the two references. In 1KGP, 90.58% of 36,817,799 SNPs detected were not measured in HapMap. More SNPs with minor allele frequencies less than 0.01 were found in 1KGP than HapMap. The two references have low disc ordance (generally smaller than 0.02) in genotypes of common SNPs, with most discordance from heterozygous SNPs. Our study demonstrated that SNP array-based GWAS findings were reliable and useful, although only a small portion of genetic variances were explained. NGS can detect not only common but also rare variants, supporting the expectation that NGS-based GWAS will be able to incorporate a much larger portion of genetic variance than SNP arrays-based GWAS.
authors.affiliation: National Center for Toxicological Research, US Food and Drug Administration, 3900 NCTR Road, Jefferson, AR 72079, USA. huixiao.hong@fda.hhs.gov.
authors.forename: Wenqian
authors.initials: W
authors.lastname: Zhang
authors.forename: Hui Wen
authors.initials: HW
authors.lastname: Ng
authors.forename: Mao
authors.initials: M
authors.lastname: Shu
authors.forename: Heng
authors.initials: H
authors.lastname: Luo
authors.forename: ZhenQiang
authors.initials: Z
authors.lastname: Su
authors.forename: Weigong
authors.initials: W
authors.lastname: Ge
authors.forename: Roger
authors.initials: R
authors.lastname: Perkins
authors.forename: Weida
authors.initials: W
authors.lastname: Tong
authors.forename: Huixiao
authors.initials: H
authors.lastname: Hong
mesh_terms: D002874:Chromosome Mapping / Q000379:methods; D005787:Gene Frequency / Q000235:genetics; D015894:Genome, Human / Q000235:genetics*; D005838:Genotype; D060148:HapMap Project; D006801:Humans; D015810:Linkage Disequilibrium / Q000235:genetics; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*