title: Kineococcus mangrovisp. nov., isolated from mangrove sediment.
abstract: An aerobic, Gram-stain-positive, motile, coccus-shaped actinobacterium, designated strain L2-1-L1T, was isolated from mangrove sediment in Thailand. The organism was deep orange, oxidase-negative and catalase-positive. Growth occurred at temperatures between 17 and 32 °C and with NaCl concentrations up to 10 %. Chemotaxonomic characteristics of strain L2-1-L1Twere typical of the genusKineococcus. The diagnostic diamino acid of the peptidoglycan wasmeso-diaminopimelic acid. Whole-cell hydrolysates contained arabinose, galactose, glucose, mannose and ribose. The menaquinone was MK-9(H2). Mycolic acids were not detected. Anteiso-C15 : 0and iso-C14 : 0were detected as the major cellular fatty acids. The major polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol and an unidentified phosphoglycolipid. The G+C content of the DNA was 74.7 mol%. A phylogenetic tree, based on 16S rRNA gene sequences, revealed that strain L2-1-L1Trepresents a novel member of the genusKineococcus. The most closely related species wereKineococcus endophyticaKLBMP 1274T,Kineococcus aurantiacusNBRC 15268TandKineococcus rhizosphaeraeRP-B16T, with 98.9, 98.6 and 98.5 % 16S rRNA gene sequence similarity, respectively. On the basis of phylogenetic analysis, DNA-DNA relatedness data, phenotypic characteristics and chemotaxonomic data, a novel species of the genusKineococcusis proposed:Kineococcus magrovisp. nov. The type strain is strain L2-1-L1T( = BCC 75409T= NBRC 110933T).
authors.affiliation: Department of Microbiology, Faculty of Science, Kasetsart University, Chatuchak, Bangkok 10900, Thailand.|Center for Advanced Studies in Tropical Natural Resources, NRU-KU, Kasetsart University, Chatuchak, Bangkok 10900, Thailand.
authors.forename: Kannika
authors.initials: K
authors.lastname: Duangmal
authors.affiliation: Department of Microbiology, Faculty of Science, Kasetsart University, Chatuchak, Bangkok 10900, Thailand.
authors.forename: Supattra
authors.initials: S
authors.lastname: Muangham
authors.affiliation: Kitasato Institute for Life Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan.
authors.forename: Ratchanee
authors.initials: R
authors.lastname: Mingma
authors.affiliation: Department of Microbiology, Faculty of Science, Kasetsart University, Chatuchak, Bangkok 10900, Thailand.
authors.forename: Thanakorn
authors.initials: T
authors.lastname: Yimyai
authors.affiliation: Department of Microbiology, Faculty of Science, Kasetsart University, Chatuchak, Bangkok 10900, Thailand.|Center for Advanced Studies in Tropical Natural Resources, NRU-KU, Kasetsart University, Chatuchak, Bangkok 10900, Thailand.
authors.forename: Nantana
authors.initials: N
authors.lastname: Srisuk
authors.affiliation: Department of Microbiology, Faculty of Science, Kasetsart University, Chatuchak, Bangkok 10900, Thailand.|Center for Advanced Studies in Tropical Natural Resources, NRU-KU, Kasetsart University, Chatuchak, Bangkok 10900, Thailand.
authors.forename: Vichien
authors.initials: V
authors.lastname: Kitpreechavanich
authors.affiliation: Kitasato Institute for Life Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan.
authors.forename: Atsuko
authors.initials: A
authors.lastname: Matsumoto
authors.affiliation: Kitasato Institute for Life Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Takahashi