title: Methyl jasmonate inhibits lamina joint inclination by repressing brassinosteroid biosynthesis and signaling in rice.
abstract: Lamina joint inclination or leaf angle (the angle between the leaf blade and vertical culm) is a major trait of rice plant architecture. The plant hormone brassinosteroid (BR) is the main regulator of this trait, while other plant hormones, including ethylene, gibberellin, and auxin, also influence leaf angle. In this study, we found that methyl jasmonate (MeJA) also participates in regulating lamina joint inclination. MeJA decreased lamina joint inclination and inhibited the BR-induced increase in lamina joint inclination. Furthermore, addition of a BR synthesis inhibitor increased the extent of change in lamina joint inclination in response to treatment with a low concentration of MeJA (0.05 or 0.5mgL(-1)), but it did not alter the lamina joint inclination of plants treated with a high concentration of MeJA (5mgL(-1)). Further studies showed that MeJA treatment significantly repressed the expression of BR biosynthesis-related genes and decreased endogenous BRs levels. In addition, the lamina joint inclination in the OsBRI1 mutant d61-1 was less sensitive to MeJA compared with its wild type counterpart, and lithium chloride-induced inactivation of GSK3-like kinase, a negative regulator of BR signaling, partly rescued the MeJA-induced reduction in lamina joint inclination. Further studies showed that MeJA treatment reduced the mRNA levels of BR signaling and target genes. These results indicate that MeJA-inhibition of lamina joint inclination may depend on BR biosynthesis and the BR signaling pathway.
authors.affiliation: College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: ganlj@njau.edu.cn.
authors.forename: Lijun
authors.initials: L
authors.lastname: Gan
authors.affiliation: College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Wu
authors.affiliation: Key Laboratory of Separation Science for Analytical Chemistry, Chinese Academy of Sciences, Dalian Institute of Chemical Physics, Dalian 116023, China.
authors.forename: Dapeng
authors.initials: D
authors.lastname: Wu
authors.affiliation: College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Zhanfang
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Zhengfei
authors.initials: Z
authors.lastname: Guo
authors.affiliation: College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Na
authors.initials: N
authors.lastname: Yang
authors.affiliation: College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Xia
authors.affiliation: College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Xie
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Department of Biotechnology, Faculty of Bioresource Sciences, Akita Prefectural University, 241-438, Shimoshinjo Nakano, Akita 010-0195, Japan.
authors.forename: Keimei
authors.initials: K
authors.lastname: Oh
authors.affiliation: Bioscience and Biotechnology Center, Nagoya University, Chikusa, Nagoya 464-8601, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Matsuoka
authors.affiliation: CH BIOTECH R&D CO., LTD. No.121, Xian'an Rd., Xianxi Township, Changhua County 50741, Taiwan, ROC.
authors.forename: Denny
authors.initials: D
authors.lastname: Ng
authors.affiliation: College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: zch@njau.edu.cn.
authors.forename: Changhua
authors.initials: C
authors.lastname: Zhu
mesh_terms: D000085:Acetates / Q000378:metabolism*; D060406:Brassinosteroids / Q000378:metabolism*; D003517:Cyclopentanes / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D054883:Oxylipins / Q000378:metabolism*; D010937:Plant Growth Regulators / Q000378:metabolism*; D018515:Plant Leaves / Q000235:genetics / Q000254:growth & development; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D015398:Signal Transduction
chemical_list: D000085:Acetates; D060406:Brassinosteroids; D003517:Cyclopentanes; D054883:Oxylipins; D010937:Plant Growth Regulators; D010940:Plant Proteins; C072239:methyl jasmonate
keywords: Brassinosteroid; Lamina joint inclination; Methyl jasmonate; Rice