title: Immunohistochemical Analysis of E-Cadherin, p53 and Inhibin-α Expression in Hydatidiform Mole and Hydropic Abortion.
abstract: The purpose of this study was to investigate the role of E-cadherin, p53, and inhibin-α immunostaining in the differential diagnosis of hydropic abortion (HA), partial hydatidiform mole (PHM), and complete hydatidiform mole (CHM). E-cadherin, p53, and inhibin-α protein expression patterns were investigated immunohistochemically using paraffin -embedded tissue sections from histologically diagnosed cases of HA (n = 23), PHM (n = 24), and CHM (n = 23). Expression patterns of these markers were scored semi-quantitatively according to the staining intensity, percentage of positive cells, and immunoreactivity score. Classification of cases was established on histologic criteria and supported by the molecular genotyping. Immunostaining allowed the identification of specific cell types with E-cadherin, p53, and inhibin-α expression in all cases. E-cadherin expression was detected on the cell surface of villous cytotrophoblasts. We observed a marked decline in the expression of E-cadherin from HAs to PHMs to CHMs. The p53-positive reaction was restricted to the nucleus of villous cytotrophoblasts. Significantly increased p53 expression was observed in CHMs, compared with HAs and PHMs. The expression of inhibin-α was localised in the cytoplasm of villous syncytiotrophoblasts, and the expression of this marker was significantly higher in PHMs and CHMs than HAs. In conclusion, immunohistochemical analysis of E-cadherin, p53, and inhibin-α expression could serve as a useful adjunct to conventional methods in the differential diagnosis of HA, PHM, and CHM.
authors.affiliation: Department of Obstetrics and Gynecology, Antalya Training and Research Hospital, Antalya, Turkey. dronurerol@hotmail.com.
authors.forename: Onur
authors.initials: O
authors.lastname: Erol
authors.affiliation: Department of Pathology, Antalya Training and Research Hospital, Antalya, Turkey.
authors.forename: Dinç
authors.initials: D
authors.lastname: Süren
authors.affiliation: Department of Pathology, Antalya Training and Research Hospital, Antalya, Turkey.
authors.forename: Birsel
authors.initials: B
authors.lastname: Tutuş
authors.affiliation: Department of Gynecologic Oncology, Antalya Training and Research Hospital, Antalya, Turkey.
authors.forename: Tayfun
authors.initials: T
authors.lastname: Toptaş
authors.affiliation: Department of Pathology, Antalya Training and Research Hospital, Antalya, Turkey.
authors.forename: Ahmet Arda
authors.initials: AA
authors.lastname: Gökay
authors.affiliation: Department of Obstetrics and Gynecology, Antalya Training and Research Hospital, Antalya, Turkey.
authors.forename: Aysel Uysal
authors.initials: AU
authors.lastname: Derbent
authors.affiliation: Department of Obstetrics and Gynecology, Antalya Training and Research Hospital, Antalya, Turkey.
authors.forename: Mustafa Kemal
authors.initials: MK
authors.lastname: Özel
authors.affiliation: Department of Pathology, Antalya Training and Research Hospital, Antalya, Turkey.
authors.forename: Cem
authors.initials: C
authors.lastname: Sezer
mesh_terms: D000022:Abortion, Spontaneous / Q000378:metabolism*; D000293:Adolescent; D000328:Adult; D015703:Antigens, CD; D015415:Biomarkers / Q000378:metabolism; D015820:Cadherins / Q000378:metabolism*; D003937:Diagnosis, Differential; D005260:Female; D006801:Humans; D006828:Hydatidiform Mole / Q000378:metabolism*; D007150:Immunohistochemistry / Q000379:methods; D007265:Inhibins / Q000378:metabolism*; D008875:Middle Aged; D011247:Pregnancy; D014327:Trophoblasts / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*; D014594:Uterine Neoplasms / Q000378:metabolism*; D055815:Young Adult
chemical_list: D015703:Antigens, CD; D015415:Biomarkers; C513384:CDH1 protein, human; D015820:Cadherins; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; C094377:inhibin-alpha subunit; D007265:Inhibins
keywords: E-cadherin; Hydatidiform mole; Inhibin-α; p53