title: Pharmacological Evaluation of Antidepressant-Like Effect of Genistein and Its Combination with Amitriptyline: An Acute and Chronic Study.
abstract: The present study was designed to evaluate the acute and chronic antidepressant effect of genistein in combination with amitriptyline in mice. Animals were divided into six groups (n = 6) for treatment with water, genistein, or amitriptyline, either alone or in combination for ten days. Animals were subjected to locomotor activity testing; tail suspension test (TST); and forced swim test (FST) and immobility time was recorded on day one and day ten. Acute treatment of all treatment groups did not significantly reduce the immobility time (p > 0.05). Chronic treatment of combination of genistein (10 mg/kg) and amitriptyline (5 mg/kg and 10 mg/kg) significantly reduced the immobility time as compared to control group (p < 0.001) and was comparable to amitriptyline alone (10 mg/kg). However, no changes in anti-immobility activity in combination of subeffective doses of genistein (5 mg/kg) and amitriptyline (5 mg/kg) were observed. Genistein at its standard dose (10 mg/kg) rendered synergistic effects in combination with subeffective dose of amitriptyline (5 mg/kg) and additive effects in combination with therapeutic dose of amitriptyline (10 mg/kg).
authors.affiliation: Department of Life Science, School of Pharmacy, International Medical University, Bukit Jalil, Kuala Lumpur 57000, Malaysia ; School of Medicine and Public Health, University of Newcastle, Newcastle, NSW 2308, Australia.
authors.forename: Gaurav
authors.initials: G
authors.lastname: Gupta
authors.affiliation: Department of Life Science, School of Pharmacy, International Medical University, Bukit Jalil, Kuala Lumpur 57000, Malaysia.
authors.forename: Tay
authors.initials: T
authors.lastname: Jia Jia
authors.affiliation: Department of Life Science, School of Pharmacy, International Medical University, Bukit Jalil, Kuala Lumpur 57000, Malaysia.
authors.forename: Lim
authors.initials: L
authors.lastname: Yee Woon
authors.affiliation: Department of Life Science, School of Pharmacy, International Medical University, Bukit Jalil, Kuala Lumpur 57000, Malaysia.
authors.forename: Dinesh
authors.initials: D
authors.lastname: Kumar Chellappan
authors.affiliation: Department of Life Science, School of Pharmacy, International Medical University, Bukit Jalil, Kuala Lumpur 57000, Malaysia.
authors.forename: Mayuren
authors.initials: M
authors.lastname: Candasamy
authors.affiliation: School of Biomedical Science, University of Newcastle, Newcastle, NSW 2308, Australia.
authors.forename: Kamal
authors.initials: K
authors.lastname: Dua