title: A novel approach to study oxidative stress in neonatal respiratory distress syndrome.
abstract: BACKGROUND
  Respiratory distress syndrome of the neonate (neonatal RDS) is still an important problem in treatment of preterm infants. It is accompanied by inflammatory processes with free radical generation and oxidative stress. The aim of study was to determine the role of oxidative stress in the development of neonatal RDS.
  
  
  METHODS
  Markers of oxidative stress and antioxidant activity in umbilical cord blood were studied in infants with neonatal respiratory distress syndrome with reference to healthy newborns.
  
  
  RESULTS
  Status of markers of oxidative stress (malondialdehyde, protein carbonyl and 8-hydroxy-2-deoxy guanosine) showed a significant increase with depleted levels of total antioxidant capacity in neonatal RDS when compared to healthy newborns.
  
  
  CONCLUSIONS
  The study provides convincing evidence of oxidative damage and diminished antioxidant defenses in newborns with RDS. Neonatal RDS is characterized by damage of lipid, protein and DNA, which indicates the augmentation of oxidative stress.
  
  
  CONCLUSIONS
  The identification of the potential biomarker of oxidative stress consists of a promising strategy to study the pathophysiology of neonatal RDS.
authors.affiliation: Department of Biophysics, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, India.
authors.forename: Reena
authors.initials: R
authors.lastname: Negi
authors.affiliation: Department of Biophysics, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, India.
authors.forename: Deepti
authors.initials: D
authors.lastname: Pande
authors.affiliation: Department of Biophysics, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, India.
authors.forename: Kanchan
authors.initials: K
authors.lastname: Karki
authors.affiliation: Department of Pediatrics, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, India.
authors.forename: Ashok
authors.initials: A
authors.lastname: Kumar
authors.affiliation: Department of Chemistry, Faculty of Science, Banaras Hindu University, Varanasi 221005, India.
authors.forename: Ranjana S
authors.initials: RS
authors.lastname: Khanna
authors.affiliation: Department of Biophysics, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, India.
authors.forename: Hari D
authors.initials: HD
authors.lastname: Khanna
keywords: Antioxidant; Free radical; Neonatal respiratory distress syndrome; Oxidative stress