title: In vitro screening of Cymbopogon jwarancusa and Conyza canadensis against liver flukes.
abstract: Aim of present study was to screen medicinal plants for flukicidal activity in vitro to develop alternative sources of treatment for trematodes infection. For this purpose, crude methanolic extracts (CME) of Cymbopogn jwarancusa and Conyza canadensis were prepared and live adult flukes viz; Fasciola gigantica, and Paramphistomum cervi isolated from liver and bile ducts of slaughtered buffalo were subjected to different drug concentrations as well as positive and negative control. Motility inhibition and paralysis leading to the death of parasites was considered as flukicidal activity of plants extracts. The results revealed that CME of C. jwarancusa and C. canadensis showed significant (P<0.05) flukicidal activity compared to positive control. Also there was a significant effect of different concentrations (P<0.05) and exposure of time on the flukes (P<0.05). Furthermore, ED50 for C. jwarancusa and C. canadensis against F. gigantica were 13.1 and 41.4 mg/ml, respectively. In the case of P. cervi, it was 10.8 and 29.0 mg/ml. It can be concluded that both tested plants showed greater flukicidal activity as compared to positive control with Albendazole till the 8(th) hr. These potent plants needs further studies invivo to elucidate their mode of action.
authors.affiliation: Department of Biology, Pir Mehr Ali shah-Arid Agriculture University, Rawalpindi 4600, Pakistan.
authors.forename: Andeela
authors.initials: A
authors.lastname: Shafiq
authors.affiliation: Department of Zoology, Pir Mehr Ali shah-Arid Agriculture University, Rawalpindi 4600, Pakistan.
authors.forename: Rabia
authors.initials: R
authors.lastname: Kanwal
authors.affiliation: Department of Botany, Pir Mehr Ali shah-Arid Agriculture University, Rawalpindi 4600, Pakistan.
authors.forename: Rahamat
authors.initials: R
authors.lastname: Ullah Qureshi
authors.affiliation: Department of Zoology, Pir Mehr Ali shah-Arid Agriculture University, Rawalpindi 4600, Pakistan.
authors.forename: Farhana
authors.initials: F
authors.lastname: Riaz Chaudhry
mesh_terms: D015766:Albendazole / Q000494:pharmacology; D000818:Animals; D000871:Anthelmintics / Q000302:isolation & purification / Q000494:pharmacology*; D035401:Conyza / Q000737:chemistry*; D031743:Cymbopogon / Q000737:chemistry*; D004353:Drug Evaluation, Preclinical; D005209:Fasciola / Q000187:drug effects* / Q000502:physiology; D008124:Locomotion / Q000187:drug effects; D010250:Paramphistomatidae / Q000187:drug effects* / Q000502:physiology; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D016019:Survival Analysis
chemical_list: D000871:Anthelmintics; D010936:Plant Extracts; D015766:Albendazole