title: Colorimetric and fluorometric monitoring of the helix composition of collagen-like peptides at the nM level.
abstract: We have demonstrated that the incorporation of a dye-labeled collagen-like peptide in the homotrimeric versus heterotrimeric context results in visible color changes and distinct fluorescence. The unique fluorescence self-quenching assay can unambiguously determine the helix composition of heterotrimers at the nM level, far extending our capability to characterize a collagen triple helix.
authors.affiliation: State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, P. R. China. xiaojx@lzu.edu.cn.
authors.forename: Xiuxia
authors.initials: X
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, P. R. China. xiaojx@lzu.edu.cn.
authors.forename: Jun
authors.initials: J
authors.lastname: Fan
authors.affiliation: State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, P. R. China. xiaojx@lzu.edu.cn.
authors.forename: Xuan
authors.initials: X
authors.lastname: Li
authors.affiliation: State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, P. R. China. xiaojx@lzu.edu.cn.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, P. R. China. xiaojx@lzu.edu.cn.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, P. R. China. xiaojx@lzu.edu.cn.
authors.forename: Jianxi
authors.initials: J
authors.lastname: Xiao
mesh_terms: D002942:Circular Dichroism; D003094:Collagen / Q000737:chemistry*; D003124:Colorimetry / Q000379:methods*; D019906:Nuclear Magnetic Resonance, Biomolecular; D010455:Peptides / Q000737:chemistry*
chemical_list: D010455:Peptides; D003094:Collagen