title: Effect of Guibi-Tang, a Traditional Herbal Formula, on Retinal Neovascularization in a Mouse Model of Proliferative Retinopathy.
abstract: Ocular pathologic angiogenesis is an important causative risk factor of blindness in retinopathy of prematurity, proliferative diabetic retinopathy, and neovascular macular degeneration. Guibi-tang (GBT) is a frequently used oriental herbal formula in East Asian countries, and is also called Qui-pi-tang in Chinese and Kihi-To in Japanese. In the present study, we investigated the preventive effect of GBT on retinal pathogenic neovascularization in a mouse model of oxygen-induced retinopathy (OIR). C57BL/6 mice were exposed to 75% hyperoxia for five days on postnatal day 7 (P7). The mice were then exposed to room air from P12 to P17 to induce ischemic proliferative retinopathy. GBT (50 or 100 mg/kg/day) was intraperitoneally administered daily for five days (from P12 to P16). On P17, Retinal neovascularization was measured on P17, and the expression levels of 55 angiogenesis-related factors were analyzed using protein arrays. GBT significantly decreased retinal pathogenic angiogenesis in OIR mice, and protein arrays revealed that GBT decreased PAI-1 protein expression levels. Quantitative real-time PCR revealed that GBT reduced vascular endothelial growth factor (VEGF), fibroblast growth factor 2 (FGF2), and plasminogen activator inhibitor 1 (PAI-1) mRNA levels in OIR mice. GBT promotes potent inhibitory activity for retinal neovascularization by decreasing VEGF, FGF2, and PAI-1 levels.
authors.affiliation: KM Convergence Research Division, Korea Institute of Oriental Medicine, Daejeon 34054, Korea. candykong@kiom.re.kr.
authors.forename: Yun Mi
authors.initials: YM
authors.lastname: Lee
authors.affiliation: KM Convergence Research Division, Korea Institute of Oriental Medicine, Daejeon 34054, Korea. yrsanta@kiom.re.kr.
authors.forename: Yu-Ri
authors.initials: YR
authors.lastname: Lee
authors.affiliation: KM Convergence Research Division, Korea Institute of Oriental Medicine, Daejeon 34054, Korea. chskim@kiom.re.kr.
authors.forename: Chan-Sik
authors.initials: CS
authors.lastname: Kim
authors.affiliation: KM Convergence Research Division, Korea Institute of Oriental Medicine, Daejeon 34054, Korea. jopd7414@kiom.re.kr.
authors.forename: Kyuhyung
authors.initials: K
authors.lastname: Jo
authors.affiliation: KM Convergence Research Division, Korea Institute of Oriental Medicine, Daejeon 34054, Korea. ssen4022@kiom.re.kr.
authors.forename: Eunjin
authors.initials: E
authors.lastname: Sohn
authors.affiliation: KM Convergence Research Division, Korea Institute of Oriental Medicine, Daejeon 34054, Korea. jskim@kiom.re.kr.
authors.forename: Jin Sook
authors.initials: JS
authors.lastname: Kim
authors.affiliation: KM Convergence Research Division, Korea Institute of Oriental Medicine, Daejeon 34054, Korea. dvmhyun@kiom.re.kr.
authors.forename: Junghyun
authors.initials: J
authors.lastname: Kim
mesh_terms: D043925:Angiogenesis Inducing Agents / Q000378:metabolism; D000818:Animals; D004195:Disease Models, Animal; D015536:Down-Regulation / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000494:pharmacology / Q000627:therapeutic use*; D016222:Fibroblast Growth Factor 2 / Q000235:genetics / Q000378:metabolism; D008810:Mice, Inbred C57BL; D010100:Oxygen; D017395:Plasminogen Activator Inhibitor 1 / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D012160:Retina / Q000187:drug effects / Q000473:pathology; D015861:Retinal Neovascularization / Q000150:complications* / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism; D018630:Vitreoretinopathy, Proliferative / Q000150:complications* / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology
chemical_list: D043925:Angiogenesis Inducing Agents; D004365:Drugs, Chinese Herbal; D017395:Plasminogen Activator Inhibitor 1; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A; C000611222:guibi-tang; D016222:Fibroblast Growth Factor 2; D010100:Oxygen
keywords: fibroblast growth factor 2; oxygen-induced retinopathy; plasminogen activator inhibitor 1; retinal neovascularization; vascular endothelial growth factor