title: Resin from Virola oleifera Protects Against Radiocontrast-Induced Nephropathy in Mice.
abstract: Contrast-induced nephropathy (CIN) is an iatrogenic medical event for which there is not yet a successful therapy. Increasing evidence in rodents has suggested that this disease is associated with renal tubular and vascular injury that is triggered directly by oxidative stress. In the present study, we evaluated whether the antioxidant resin from Virola oleifera (RV) could attenuate renal damage in an experimental mouse model of CIN. Adult male Swiss mice were divided into six groups and pre-treated orally with RV (10, 100 and 300 mg/kg), N-acetylcysteine (200 mg/kg) or vehicle for 5 days before the induction of CIN and Control group. Renal function was assessed by measuring plasma creatinine and urea levels. Additionally, renal oxidative stress and apoptosis/cell viability were determined with flow cytometry. Finally, kidney tissues were sectioned for histopathological examination. In this CIN model, pre-treatment with RV improved renal function, lowered the mortality rate, and reduced oxidative stress and apoptosis in both the medulla and cortex renal cells in a dose-dependent manner. Moreover, the RV treatment had beneficial effects on kidney histopathology that were superior to the standard treatment with N-acetylcysteine. These data suggest that because of its antioxidative and antiapoptotic effects and its ability to preserve renal function, resin from Virola oleifera may have potential as a new therapeutic approach for preventing CIN.
authors.affiliation: Pharmaceutical Sciences Graduate Program, Vila Velha University (UVV), Vila Velha, ES, Brazil.
authors.forename: Igor Santos Fonte
authors.initials: IS
authors.lastname: Bôa
authors.affiliation: Laboratory of Translational Physiology, Health Sciences Center, Federal University of Espirito Santo, Vitoria, Brazil.
authors.forename: Marcella Leite
authors.initials: ML
authors.lastname: Porto
authors.affiliation: Pharmaceutical Sciences Graduate Program, Vila Velha University (UVV), Vila Velha, ES, Brazil.
authors.forename: Ana Claudia Hertel
authors.initials: AC
authors.lastname: Pereira
authors.affiliation: Pharmaceutical Sciences Graduate Program, Vila Velha University (UVV), Vila Velha, ES, Brazil.
authors.forename: Jean Pierre Louzada
authors.initials: JP
authors.lastname: Ramos
authors.affiliation: Pharmaceutical Sciences Graduate Program, Vila Velha University (UVV), Vila Velha, ES, Brazil.
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Scherer
authors.affiliation: Department of Morphology, Health Sciences Center, Federal University of Espirito Santo, Vitoria, Brazil.
authors.forename: Jairo Pinto
authors.initials: JP
authors.lastname: Oliveira
authors.affiliation: Department of Morphology, Health Sciences Center, Federal University of Espirito Santo, Vitoria, Brazil.
authors.forename: Breno Valentim
authors.initials: BV
authors.lastname: Nogueira
authors.affiliation: Laboratory of Translational Physiology, Health Sciences Center, Federal University of Espirito Santo, Vitoria, Brazil.
authors.forename: Silvana Santos
authors.initials: SS
authors.lastname: Meyrelles
authors.affiliation: Pharmaceutical Sciences Graduate Program, Vila Velha University (UVV), Vila Velha, ES, Brazil.|Laboratory of Translational Physiology, Health Sciences Center, Federal University of Espirito Santo, Vitoria, Brazil.
authors.forename: Elisardo Corral
authors.initials: EC
authors.lastname: Vasquez
authors.affiliation: Pharmaceutical Sciences Graduate Program, Vila Velha University (UVV), Vila Velha, ES, Brazil.|Federal Institute of Education, Science and Technology (IFES), Vila Velha, ES, Brazil.
authors.forename: Denise Coutinho
authors.initials: DC
authors.lastname: Endringer
authors.affiliation: Pharmaceutical Sciences Graduate Program, Vila Velha University (UVV), Vila Velha, ES, Brazil.|Federal Institute of Education, Science and Technology (IFES), Vila Velha, ES, Brazil.
authors.forename: Thiago Melo Costa
authors.initials: TM
authors.lastname: Pereira
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D015415:Biomarkers; D002470:Cell Survival / Q000187:drug effects; D002853:Chromatography, Liquid; D003287:Contrast Media / Q000009:adverse effects*; D004195:Disease Models, Animal; D062385:Hydroxybenzoates / Q000737:chemistry; D007674:Kidney Diseases / Q000139:chemically induced* / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology; D029562:Magnoliaceae / Q000737:chemistry*; D013058:Mass Spectrometry; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects; D020011:Protective Agents / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D012116:Resins, Plant / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D003287:Contrast Media; D062385:Hydroxybenzoates; D020011:Protective Agents; D017382:Reactive Oxygen Species; D012116:Resins, Plant; C017616:phenolic acid