title: Lactic acid bacteria in dried vegetables and spices.
abstract: Spices and dried vegetable seasonings are potential sources of bacterial contamination for foods. However, little is known about lactic acid bacteria (LAB) in spices and dried vegetables, even though certain LAB may cause food spoilage. In this study, we enumerated LAB in 104 spices and dried vegetables products aimed for the food manufacturing industry. The products were obtained from a spice wholesaler operating in Finland, and were sampled during a one-year period. We picked isolates (n = 343) for species identification based on numerical analysis of their ribotyping patterns and comparing them with the corresponding patterns of LAB type strains. We found LAB at levels >2 log CFU/g in 68 (65%) of the samples, with the highest counts detected from dried onion products and garlic powder with counts ranging from 4.24 to 6.64 log CFU/g. The LAB identified were predominantly Weissella spp. (61%) and Pediococcus spp. (15%) with Weissella confusa, Weissella cibaria, Weissella paramesenteroides, Pediococcus acidilactici and Pediococcus pentosaceus being the species identified. Other species identified belonged to the genera of Enterococcus spp. (8%), Leuconostoc spp. (6%) and Lactobacillus spp. (2%). Among the LAB identified, Leuconostoc citreum, Leuconostoc mesenteroides and W. confusa have been associated with food spoilage. Our findings suggest that spices and dried vegetables are potential sources of LAB contamination in the food industry.
authors.affiliation: Department of Food Hygiene and Environmental Health, Faculty of Veterinary Medicine, University of Helsinki, Finland. Electronic address: elina.sade@helsinki.fi.
authors.forename: Elina
authors.initials: E
authors.lastname: Säde
authors.affiliation: Department of Food Hygiene and Environmental Health, Faculty of Veterinary Medicine, University of Helsinki, Finland.
authors.forename: Elisa
authors.initials: E
authors.lastname: Lassila
authors.affiliation: Department of Food Hygiene and Environmental Health, Faculty of Veterinary Medicine, University of Helsinki, Finland.
authors.forename: Johanna
authors.initials: J
authors.lastname: Björkroth
mesh_terms: D044822:Biodiversity; D015169:Colony Count, Microbial; D019344:Lactic Acid / Q000378:metabolism; D056546:Lactobacillales / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D010802:Phylogeny; D017365:Spices / Q000032:analysis / Q000382:microbiology*; D014675:Vegetables / Q000737:chemistry / Q000382:microbiology*
chemical_list: D019344:Lactic Acid
keywords: Dried onion; Garlic powder; Lactic acid bacteria; Spices; Weissella spp.