title: Optimization of keratin/alginate scaffold using RSM and its characterization for tissue engineering.
abstract: The scaffold for tissue engineering was fabricated from a binary blend of keratin/alginate. The concentration and ratio of keratin and alginate was optimized by response surface methodology in a scaffold. The structural compatibility between keratin and alginate was examined by X-ray diffractometer and Fourier transforms infrared spectroscopy. Apparent porosity of the scaffold was calculated by Archimedes principles and its observed value of was found 96.25 ± 0.04%. The pore size of the scaffold was observed in the range between 10 and 200 μm. Tensile strength (0.33 ± 0.26 MPa) and percent of elongation at break (23.33 ± 2.52%) are the reported mechanical strength of the scaffold. Positive antimicrobial activity and in vitro degradation further confirms the fabrication of a scaffold required for tissue engineering application.
authors.affiliation: Department of Biotechnology, National Institute of Technology Raipur, Chhattisgarh 492010, India. Electronic address: pguptabiotech@gmail.com.
authors.forename: Pratima
authors.initials: P
authors.lastname: Gupta
authors.affiliation: Department of Biotechnology, National Institute of Technology Raipur, Chhattisgarh 492010, India. Electronic address: nayak23bt@gmail.com.
authors.forename: Kush Kumar
authors.initials: KK
authors.lastname: Nayak
mesh_terms: D000464:Alginates / Q000737:chemistry*; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D007633:Keratins / Q000737:chemistry*; D008422:Materials Testing; D055595:Mechanical Phenomena; D016062:Porosity; D017550:Spectroscopy, Fourier Transform Infrared; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D007633:Keratins; D020723:Glucuronic Acid
keywords: Antimicrobial activity; Mechanical behavior; Porosity; RSM; Scaffold fabrication; Structural characterization