title: Oxidative stress, neurotoxicity, and metallothionein (MT) gene expression in juvenile rock fish Sebastes schlegelii under the different levels of dietary chromium (Cr(6+)) exposure.
abstract: Juvenile Sebastes schlegelii were exposed for 4 weeks with the different levels of dietary chromium (Cr(6+)) concentration (0, 30, 60, 120 and 200mg/kg). The superoxide dismutase (SOD) activity, glutathione S-transferase (GST) activity, and glutathione (GSH) level of liver and gill were evaluated after 4 weeks exposure. The SOD and GST activity of liver and gill was significantly increased in the concentration of 240mg/kg after 2 weeks and over 120mg/kg after 4 weeks, whereas a considerable decrease in the concentration of 240mg/kg after 2 weeks and over 120mg/kg after 4 weeks was observed in the GSH levels of liver and gill. In neurotoxicity, AChE activity was significatly inhibited in brain in the concentration of 240mg/kg after 2 weeks and over 60mg/kg after 4 weeks and muscle in the concentration of 240mg/kg after 2 weeks and over 120mg/kg after 4 weeks. Metallothionein (MT) gene in liver was considerably increased over 120mg/kg after 2 weeks and at 30, 120, and 240mg/kg after 4 weeks by dietary chromium exposure. The results indicate that dietary Cr exposure over 120mg/kg can induce substantial alterations in antioxidant responses, AChE activity and MT gene expression.
authors.affiliation: Department of Aquatic Life Medicine, Pukyong National University, Busan 608-737, Republic of Korea.
authors.forename: Jun-Hwan
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Department of Aquatic Life Medicine, Pukyong National University, Busan 608-737, Republic of Korea. Electronic address: jckang@pknu.ac.kr.
authors.forename: Ju-Chan
authors.initials: JC
authors.lastname: Kang
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001921:Brain / Q000378:metabolism; D002857:Chromium / Q000633:toxicity*; D005399:Fishes / Q000378:metabolism / Q000502:physiology*; D015870:Gene Expression; D005786:Gene Expression Regulation / Q000187:drug effects*; D005880:Gills / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008668:Metallothionein / Q000235:genetics / Q000378:metabolism*; D009132:Muscles / Q000378:metabolism; D009420:Nervous System / Q000187:drug effects*; D018384:Oxidative Stress / Q000187:drug effects*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D000975:Antioxidants; D014874:Water Pollutants, Chemical; D002857:Chromium; D008668:Metallothionein; D005979:Glutathione Peroxidase; D005982:Glutathione Transferase; D000110:Acetylcholinesterase; D005978:Glutathione
keywords: Antioxidant enzymes; Chromium; Metallothionein; Neurotoxicity; Rockfish