title: Tailored and biodegradable poly(2-oxazoline) microbeads as 3D matrices for stem cell culture in regenerative therapies.
abstract: We present the synthesis of hydrogel microbeads based on telechelic poly(2-oxazoline) (POx) crosslinkers and the methacrylate monomers (HEMA, METAC, SPMA) by inverse emulsion polymerization. While in batch experiments only irregular and ill-defined beads were obtained, the preparation in a microfluidic (MF) device resulted in highly defined hydrogel microbeads. Variation of the MF parameters allowed to control the microbead diameter from 50 to 500 μm. Microbead elasticity could be tuned from 2 to 20 kPa by the POx:monomer composition, the POx chain length, net charge of the hydrogel introduced via the monomer as well as by the organic content of the aqueous phase. The proliferations of human mesenchymal stem cells (hMSCs) on the microbeads were studied. While neutral, hydrophilic POx-PHEMA beads were bioinert, excessive colonization of hMSCs on charged POx-PMETAC and POx-PSPMA was observed. The number of proliferated cells scaled roughly linear with the METAC or SPMA comonomer content. Additional collagen I coating further improved the stem cell proliferation. Finally, a first POx-based system for the preparation of biodegradable hydrogel microcarriers is described and evaluated for stem cell culturing.
authors.affiliation: Professur für Makromolekulare Chemie, Department Chemie, Technische Universität Dresden, Mommsenstr. 4, 01069, Dresden, Germany; Center for Regenerative Therapies Dresden (CRTD), Fetcherstr. 105, 01307, Dresden, Germany; Dresden Initiative for Bioactive Interfaces & Materials, Technische Universität Dresden, Mommsenstr. 4, 01069, Dresden, Germany.
authors.forename: Steffen
authors.initials: S
authors.lastname: Lück
authors.affiliation: Professur für Makromolekulare Chemie, Department Chemie, Technische Universität Dresden, Mommsenstr. 4, 01069, Dresden, Germany.
authors.forename: René
authors.initials: R
authors.lastname: Schubel
authors.affiliation: Professur für Makromolekulare Chemie, Department Chemie, Technische Universität Dresden, Mommsenstr. 4, 01069, Dresden, Germany; Dresden Initiative for Bioactive Interfaces & Materials, Technische Universität Dresden, Mommsenstr. 4, 01069, Dresden, Germany.
authors.forename: Jannick
authors.initials: J
authors.lastname: Rüb
authors.affiliation: Dresden Initiative for Bioactive Interfaces & Materials, Technische Universität Dresden, Mommsenstr. 4, 01069, Dresden, Germany; Max-Bergmann Center of Biomaterials Dresden, Budapester Str. 27, 01069, Dresden, Germany.
authors.forename: Dominik
authors.initials: D
authors.lastname: Hahn
authors.affiliation: Center for Regenerative Therapies Dresden (CRTD), Fetcherstr. 105, 01307, Dresden, Germany.
authors.forename: Evelien
authors.initials: E
authors.lastname: Mathieu
authors.affiliation: Max-Bergmann Center of Biomaterials Dresden, Budapester Str. 27, 01069, Dresden, Germany.
authors.forename: Heike
authors.initials: H
authors.lastname: Zimmermann
authors.affiliation: Max-Bergmann Center of Biomaterials Dresden, Budapester Str. 27, 01069, Dresden, Germany.
authors.forename: Dieter
authors.initials: D
authors.lastname: Scharnweber
authors.affiliation: Dresden Initiative for Bioactive Interfaces & Materials, Technische Universität Dresden, Mommsenstr. 4, 01069, Dresden, Germany; Max-Bergmann Center of Biomaterials Dresden, Budapester Str. 27, 01069, Dresden, Germany.
authors.forename: Carsten
authors.initials: C
authors.lastname: Werner
authors.affiliation: Center for Regenerative Therapies Dresden (CRTD), Fetcherstr. 105, 01307, Dresden, Germany. Electronic address: sophie.pautot@crt-dresden.de.
authors.forename: Sophie
authors.initials: S
authors.lastname: Pautot
authors.affiliation: Professur für Makromolekulare Chemie, Department Chemie, Technische Universität Dresden, Mommsenstr. 4, 01069, Dresden, Germany; Center for Regenerative Therapies Dresden (CRTD), Fetcherstr. 105, 01307, Dresden, Germany; Dresden Initiative for Bioactive Interfaces & Materials, Technische Universität Dresden, Mommsenstr. 4, 01069, Dresden, Germany. Electronic address: Rainer.Jordan@tu-dresden.de.
authors.forename: Rainer
authors.initials: R
authors.lastname: Jordan
mesh_terms: D020341:Absorbable Implants; D061249:Batch Cell Culture Techniques / Q000295:instrumentation; D001672:Biocompatible Materials / Q000138:chemical synthesis; D002470:Cell Survival; D002478:Cells, Cultured; D004867:Equipment Design; D019544:Equipment Failure Analysis; D048091:Guided Tissue Regeneration / Q000295:instrumentation*; D006801:Humans; D056656:Lab-On-A-Chip Devices; D008422:Materials Testing; D045164:Mesenchymal Stem Cell Transplantation / Q000295:instrumentation*; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008863:Microspheres; D010080:Oxazoles / Q000737:chemistry*; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials; D010080:Oxazoles; C577913:poly(2-oxazoline)
keywords: Biodegradable; Microbeads; Microfluidics; Poly(2-oxazoline); Stem cells