title: The novel elicitor AsES triggers a defense response against Botrytis cinerea in Arabidopsis thaliana.
abstract: AsES (Acremonium strictum Elicitor and Subtilisin) is a novel extracellular elicitor protein produced by the avirulent isolate SS71 of the opportunist strawberry fungal pathogen A. strictum. Here we describe the activity of AsES in the plant-pathogen system Arabidopsis thaliana-Botrytis cinerea. We show that AsES renders A. thaliana plants resistant to the necrotrophic pathogen B. cinerea, both locally and systemically and the defense response observed is dose-dependent. Systemic, but not local resistance is dependent on the length of exposure to AsES. The germination of the spores in vitro was not inhibited by AsES, implying that protection to B. cinerea is due to the induction of the plant defenses. These results were further supported by the findings that AsES differentially affects mutants impaired in the response to salicylic acid, jasmonic acid and ethylene, suggesting that AsES triggers the defense response through these three signaling pathways.
authors.affiliation: Department of Biology, University of Fribourg, Chemin du Musée 10, 1700 Fribourg, Switzerland; Instituto Superior de Investigaciones Biológicas (INSIBIO), CONICET-UNT, e Instituto de Química Biológica "Dr. Bernabé Bloj", Facultad de Bioquímica, Química y Farmacia, UNT. Chacabuco 461, T4000ILI San Miguel de Tucumán, Argentina. Electronic address: v.hael.conrad@hotmail.com.
authors.forename: V
authors.initials: V
authors.lastname: Hael-Conrad
authors.affiliation: Department of Biology, University of Fribourg, Chemin du Musée 10, 1700 Fribourg, Switzerland. Electronic address: eliane.abou-mansour@unifr.ch.
authors.forename: E
authors.initials: E
authors.lastname: Abou-Mansour
authors.affiliation: Instituto Superior de Investigaciones Biológicas (INSIBIO), CONICET-UNT, e Instituto de Química Biológica "Dr. Bernabé Bloj", Facultad de Bioquímica, Química y Farmacia, UNT. Chacabuco 461, T4000ILI San Miguel de Tucumán, Argentina. Electronic address: juan@fbqf.unt.edu.ar.
authors.forename: J-C
authors.initials: JC
authors.lastname: Díaz-Ricci
authors.affiliation: Department of Biology, University of Fribourg, Chemin du Musée 10, 1700 Fribourg, Switzerland. Electronic address: jean-pierre.metraux@unifr.ch.
authors.forename: J-P
authors.initials: JP
authors.lastname: Métraux
authors.affiliation: Department of Biology, University of Fribourg, Chemin du Musée 10, 1700 Fribourg, Switzerland; Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Avenida Universidad 2001, 62210 Cuernavaca, Morelos, Mexico. Electronic address: serrano@ccg.unam.mx.
authors.forename: M
authors.initials: M
authors.lastname: Serrano
mesh_terms: D000164:Acremonium / Q000737:chemistry*; D017360:Arabidopsis / Q000276:immunology* / Q000382:microbiology*; D020171:Botrytis / Q000187:drug effects*; D003517:Cyclopentanes / Q000378:metabolism; D060467:Disease Resistance; D005030:Ethylenes / Q000378:metabolism; D005656:Fungal Proteins / Q000494:pharmacology*; D054883:Oxylipins / Q000378:metabolism; D010935:Plant Diseases / Q000382:microbiology / Q000517:prevention & control*; D010937:Plant Growth Regulators / Q000378:metabolism; D057865:Plant Immunity; D020156:Salicylic Acid / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D003517:Cyclopentanes; D005030:Ethylenes; D005656:Fungal Proteins; D054883:Oxylipins; D010937:Plant Growth Regulators; C011006:jasmonic acid; C036216:ethylene; D020156:Salicylic Acid
keywords: Arabidopsis thaliana; AsES; Botrytis cinerea; Elicitor; Plant defense