title: A bimodal molecular imaging probe based on chitosan encapsulated magneto-fluorescent nanocomposite offers biocompatibility, visualization of specific cancer cells in vitro and lung tissues in vivo.
abstract: Multifunctional hybrid nanocomposite material, consists of chitosan encapsulated iron oxide (as MRI contrasting agent), CdS (as fluorescent probe) nanoparticles and podophyllotoxin (as anticancer drug) was synthesized and characterized. The TEM studies suggested the size of the NPs to be in the range of 80-100 nm. These nanocomposites were treated with different cancer cell lines viz., KB, C6 and A549 cells. Fluorescence imaging and Perl's Prussian blue staining confirmed the presence of these nanocomposites inside both KB and C6 cells but not in A549 cells. Cytotoxicity experiments revealed that these biopolymer coated nanocomposites showed minimal toxicity towards cancerous cells. Further the intraperitoneal administration of one of the nanoformulations to Wistar rats suggested deposition of these nanocomposites in the lungs. The hematological, biochemical and histopathological analysis confirmed that these nanocomposites are safe to use as a novel dual mode imaging material.
authors.affiliation: Biotechnology Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur, HP 176061, India; Academy of Scientific & Innovative Research (AcSIR), New Delhi, India.
authors.forename: Shanka
authors.initials: S
authors.lastname: Walia
authors.affiliation: Pharmacology and Toxicology Laboratory, Food Nutraceutical and Quality Control Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur, HP 176061, India; Academy of Scientific & Innovative Research (AcSIR), New Delhi, India.
authors.forename: Supriya
authors.initials: S
authors.lastname: Sharma
authors.affiliation: Pharmacology and Toxicology Laboratory, Food Nutraceutical and Quality Control Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur, HP 176061, India.
authors.forename: Pankaj
authors.initials: P
authors.lastname: Markand Kulurkar
authors.affiliation: Pharmacology and Toxicology Laboratory, Food Nutraceutical and Quality Control Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur, HP 176061, India; Academy of Scientific & Innovative Research (AcSIR), New Delhi, India. Electronic address: vikrampatial@ihbt.res.in.
authors.forename: Vikram
authors.initials: V
authors.lastname: Patial
authors.affiliation: Biotechnology Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur, HP 176061, India; Academy of Scientific & Innovative Research (AcSIR), New Delhi, India. Electronic address: amitabhachem@gmail.com.
authors.forename: Amitabha
authors.initials: A
authors.lastname: Acharya
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000378:metabolism; D045744:Cell Line, Tumor; D048271:Chitosan / Q000737:chemistry* / Q000378:metabolism; D005260:Female; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D008168:Lung / Q000737:chemistry / Q000378:metabolism; D008297:Male; D057054:Molecular Imaging / Q000379:methods*; D015335:Molecular Probes / Q000737:chemistry* / Q000378:metabolism; D053761:Nanocomposites / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000970:Antineoplastic Agents; D005456:Fluorescent Dyes; D015335:Molecular Probes; D048271:Chitosan
keywords: Chitosan nanoparticles; Fluorescence imaging; Hematology; Histopathology; Perl’s Prussian blue staining