title: Genetic diversity of HPV16 and HPV18 in Brazilian patients with invasive cervical cancer.
abstract: Cervical cancer is the fourth most common cancer among women, and ∼70-80% of these cancers are associated with two human papillomavirus types: HPV16 and HPV18. Several studies have reported that intra-type diversity is associated with the progression of infection to invasive cancer. Herein, we report the genetic diversity of HPV16 and HPV18 in a cohort of 594 Brazilian women with invasive cervical cancer and describe the prevalence of lineages and intra-type diversity prior to the implementation of the public immunization program in Brazil. HPV detection and genotyping were performed using PCR, PGMY/GP primers, and DNA extracted from fresh tumors. The HPV16 (378 women) and HPV18 (80 women) lineages were identified by PCR and sequencing of the LCR and E6 fragments, followed by SNV comparison and phylogenetic analysis. In our cohort, was found a higher frequency of the lineage A (in 217 women), followed by lineage D (in 97 women) and lineages B and C (in 10 women each) for HPV16; and a higher frequency of lineage A (in 56 women) followed by lineage B (in 15 women) in HPV18. The genetic diversity of HPV16 indicated a recent expansion of specific variants or a selective advantage that is associated with invasive cancer; this pattern was not observed for HPV18.
authors.affiliation: Genetics Program, National Cancer Institute José de Alencar Gomes da Silva, Brazil.
authors.forename: Joao Paulo C B
authors.initials: JP
authors.lastname: Vidal
authors.affiliation: Genetics Program, National Cancer Institute José de Alencar Gomes da Silva, Brazil.
authors.forename: Shayany Pinto
authors.initials: SP
authors.lastname: Felix
authors.affiliation: Genetics Program, National Cancer Institute José de Alencar Gomes da Silva, Brazil.
authors.forename: Cláudia B P
authors.initials: CB
authors.lastname: Chaves
authors.affiliation: Gynecologic Oncology Department, Cancer Hospital II, National Cancer Institute José de Alencar Gomes da Silva, Brazil.
authors.forename: Patrícia
authors.initials: P
authors.lastname: Patury
authors.affiliation: Gynecologic Oncology Department, Cancer Hospital II, National Cancer Institute José de Alencar Gomes da Silva, Brazil.
authors.forename: Vanessa F
authors.initials: VF
authors.lastname: Franco
authors.affiliation: Gynecologic Oncology Department, Cancer Hospital II, National Cancer Institute José de Alencar Gomes da Silva, Brazil.
authors.forename: Evaneide A
authors.initials: EA
authors.lastname: de Morais
authors.affiliation: Populational Research Department, National Cancer Institute José de Alencar Gomes da Silva, Brazil.
authors.forename: Neile A
authors.initials: NA
authors.lastname: de Carvalho
authors.affiliation: Populational Research Department, National Cancer Institute José de Alencar Gomes da Silva, Brazil.
authors.forename: Aurenice C L
authors.initials: AC
authors.lastname: Carvalho
authors.affiliation: Gynecologic Oncology Department, Cancer Hospital II, National Cancer Institute José de Alencar Gomes da Silva, Brazil.
authors.forename: Olimpio F
authors.initials: OF
authors.lastname: Almeida Neto
authors.affiliation: Gynecologic Oncology Department, Cancer Hospital II, National Cancer Institute José de Alencar Gomes da Silva, Brazil.
authors.forename: Lina Maria T M
authors.initials: LM
authors.lastname: Vieira
authors.affiliation: Gynecologic Oncology Department, Cancer Hospital II, National Cancer Institute José de Alencar Gomes da Silva, Brazil.
authors.forename: Flavia Miranda
authors.initials: FM
authors.lastname: Correa
authors.affiliation: Populational Research Department, National Cancer Institute José de Alencar Gomes da Silva, Brazil.
authors.forename: Luís Felipe Leite
authors.initials: LF
authors.lastname: Martins
authors.affiliation: Populational Research Department, National Cancer Institute José de Alencar Gomes da Silva, Brazil.
authors.forename: Antonio
authors.initials: A
authors.lastname: Negrão
authors.affiliation: Populational Research Department, National Cancer Institute José de Alencar Gomes da Silva, Brazil.
authors.forename: Liz Maria
authors.initials: LM
authors.lastname: de Almeida
authors.affiliation: Genetics Program, National Cancer Institute José de Alencar Gomes da Silva, Brazil.
authors.forename: Miguel Angelo Martins
authors.initials: MA
authors.lastname: Moreira
mesh_terms: D000328:Adult; D000368:Aged; D001938:Brazil / Q000453:epidemiology; D004279:DNA, Viral / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics; D005260:Female; D014644:Genetic Variation; D016679:Genome, Viral; D005838:Genotype; D052162:Human papillomavirus 16 / Q000235:genetics* / Q000302:isolation & purification; D052161:Human papillomavirus 18 / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009856:Oncogene Proteins, Viral / Q000235:genetics; D010802:Phylogeny; D015995:Prevalence; D012097:Repressor Proteins / Q000235:genetics; D012307:Risk Factors; D002583:Uterine Cervical Neoplasms / Q000453:epidemiology / Q000473:pathology / Q000821:virology*; D055815:Young Adult
chemical_list: D004279:DNA, Viral; D004268:DNA-Binding Proteins; C068651:E6 protein, Human papillomavirus type 16; C052603:E6 protein, Human papillomavirus type 18; D009856:Oncogene Proteins, Viral; D012097:Repressor Proteins
keywords: HPV genetic diversity; HPV lineages; human papillomavirus type 16; human papillomavirus type 18