title: Analysis of molecular epidemiologic characteristics of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli colonizing feces in hospital patients and community dwellers in a Japanese city.
abstract: Infectious diseases caused by extended-spectrum β-lactamase (ESBL)-producing Escherichia coli are prevalent because of nosocomial infection. In addition, colonization of ESBL-producing E. coli in the intestinal tract of community dwellers due to the contamination of meat or environmental water is assumed to be one of the sources, but the causes have not been clarified. To analyze these factors, we investigated the difference in clonal groups using a combination of phylogenetic groups and multilocus sequence typing of ESBL-producing E. coli, which were obtained from the feces of an inpatient group in our hospital and a community-dwelling group living in a Japanese city. The carriage rate of ESBL-producing E. coli in the inpatient group was 12.5% (32/257), similar to that of 8.5% (42/496) in the community dwellers (P = 0.082). Of the ESBL clonal groups detected from the community dwellers, 52% (22/42) were clonal groups, including D-ST1485, D-ST70, D-ST2847, B2-ST550, B2-ST3510, A-ST93, A-ST580, A-ST716 and B1-ST2787, that have not been detected from human pathogens, meat, companion animals and environmental water, whereas all clonal groups detected from the inpatients were those that had already been reported. The rate of fluoroquinolone-resistant ESBL clonal groups colonizing the intestinal tract of the inpatient group rose as the number of hospital days increased. These results indicated that different factors were related to colonization of ESBL-producing E. coli in the feces of the inpatient group and the community-dwelling group.
authors.affiliation: Division of Clinical Bacteriology, Department of Laboratory Medicine, Tenri Hospital, Japan; Department of Clinical Laboratory Science, Faculty of Health Care, Tenri Health Care University, Japan; Division of Laboratory Medicine, Kobe University Graduate School of Medicine, Japan. Electronic address: nakamurium@tenriyorozu.jp.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Nakamura
authors.affiliation: Department of Clinical Laboratory Science, Faculty of Health Care, Tenri Health Care University, Japan.
authors.forename: Masaru
authors.initials: M
authors.lastname: Komatsu
authors.affiliation: Division of Clinical Bacteriology, Department of Laboratory Medicine, Tenri Hospital, Japan.
authors.forename: Nobuyoshi
authors.initials: N
authors.lastname: Noguchi
authors.affiliation: Division of Clinical Bacteriology, Department of Laboratory Medicine, Tenri Hospital, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Ohno
authors.affiliation: Division of Clinical Bacteriology, Department of Laboratory Medicine, Tenri Hospital, Japan.
authors.forename: Eriko
authors.initials: E
authors.lastname: Hashimoto
authors.affiliation: Division of Clinical Bacteriology, Department of Laboratory Medicine, Tenri Hospital, Japan.
authors.forename: Hiroko
authors.initials: H
authors.lastname: Matsutani
authors.affiliation: Division of Clinical Bacteriology, Department of Laboratory Medicine, Tenri Hospital, Japan.
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Abe
authors.affiliation: Division of Clinical Bacteriology, Department of Laboratory Medicine, Tenri Hospital, Japan.
authors.forename: Saori
authors.initials: S
authors.lastname: Fukuda
authors.affiliation: Division of Clinical Bacteriology, Department of Laboratory Medicine, Tenri Hospital, Japan.
authors.forename: Hisashi
authors.initials: H
authors.lastname: Kohno
authors.affiliation: Division of Clinical Bacteriology, Department of Laboratory Medicine, Tenri Hospital, Japan.
authors.forename: Fumihiko
authors.initials: F
authors.lastname: Nakamura
authors.affiliation: Department of Clinical Laboratory Science, Faculty of Health Care, Tenri Health Care University, Japan.
authors.forename: Shuji
authors.initials: S
authors.lastname: Matsuo
authors.affiliation: Division of Laboratory Medicine, Kobe University Graduate School of Medicine, Japan.
authors.forename: Seiji
authors.initials: S
authors.lastname: Kawano
mesh_terms: D000328:Adult; D000368:Aged; D003428:Cross Infection / Q000382:microbiology*; D004926:Escherichia coli / Q000302:isolation & purification* / Q000378:metabolism*; D004927:Escherichia coli Infections / Q000453:epidemiology*; D005243:Feces / Q000382:microbiology*; D005260:Female; D006761:Hospitals; D006801:Humans; D057187:Independent Living; D007564:Japan / Q000453:epidemiology; D008297:Male; D017720:Molecular Epidemiology; D015995:Prevalence; D001618:beta-Lactamases / Q000378:metabolism*
chemical_list: D001618:beta-Lactamases
keywords: ESBL; Epidemiology; Escherichia coli; Molecular characterization