title: Investigation of the Effects of Perfluorooctanoic Acid (PFOA) and Perfluorooctane Sulfonate (PFOS) on Apoptosis and Cell Cycle in a Zebrafish (Danio rerio) Liver Cell Line.
abstract: This study aimed to explore the effects of perfluorooctanoic acid (PFOA) and perfluorooctane sulfonate (PFOS) on apoptosis and cell cycle in a zebrafish (Danio rerio) liver cell line (ZFL). Treatment groups included a control group, PFOA-IC(50), PFOA-IC(80), PFOS-IC50 and PFOS-IC(80) groups. IC(50) and IC(80) concentrations were identified by cellular modeling and MTT assays. mRNA levels of p53, Bcl-2, Bax, Caspase-3 and NF-κB p65 were detected by qPCR. Cell apoptosis and cell cycle were detected by flow cytometry and the protein levels of p53, Bcl-2, Bax, Caspase-3 and NF-κB p65 were determined by western blotting. Both PFOA and PFOS inhibited the growth of zebrafish liver cells, and the inhibition rate of PFOS was higher than that of PFOA. Bcl-2 expression levels in the four groups were significantly higher than the control group and Bcl-2 increased significantly in the PFOA-IC(80) group. However, the expression levels of Bax in the four treatment groups were higher than the control group. The percentage of cell apoptosis increased significantly with the treatment of PFOA and PFOS (p < 0.05). Cell cycle and cell proliferation were blocked in both the PFOA-IC(80) and PFOS-IC(80) groups, indicating that PFOA-IC(80) and PFOS-IC(50) enhanced apoptosis in ZFL cells.
authors.affiliation: Chinese Academy of Inspection and Quarantine, Beijing 100123, China. cuiy@aqsiqch.ac.cn.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Cui
authors.affiliation: Chinese Academy of Inspection and Quarantine, Beijing 100123, China. liuw@aqsiqch.ac.cn.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: Chinese Academy of Inspection and Quarantine, Beijing 100123, China. xiewp@aqsiqch.ac.cn.
authors.forename: Wenping
authors.initials: W
authors.lastname: Xie
authors.affiliation: Chinese Academy of Inspection and Quarantine, Beijing 100123, China. yuwl@aqsiqch.ac.cn.
authors.forename: Wenlian
authors.initials: W
authors.lastname: Yu
authors.affiliation: Chinese Academy of Inspection and Quarantine, Beijing 100123, China. wangz@aqsiqch.ac.cn.
authors.forename: Cheng
authors.initials: C
authors.lastname: Wang
authors.affiliation: Chinese Academy of Inspection and Quarantine, Beijing 100123, China. chenhm@aqsiqch.ac.cn.
authors.forename: Huiming
authors.initials: H
authors.lastname: Chen
mesh_terms: D017738:Alkanesulfonic Acids / Q000633:toxicity*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002210:Caprylates / Q000633:toxicity*; D002453:Cell Cycle / Q000187:drug effects*; D002460:Cell Line; D005466:Fluorocarbons / Q000633:toxicity*; D022781:Hepatocytes / Q000187:drug effects*; D008099:Liver / Q000187:drug effects*; D018675:Toxicity Tests; D015027:Zebrafish / Q000502:physiology*
chemical_list: D017738:Alkanesulfonic Acids; D002210:Caprylates; D005466:Fluorocarbons; C023036:perfluorooctanoic acid; C076994:perfluorooctane sulfonic acid
keywords: apoptosis; perfluorooctane sulfonate (PFOS); perfluorooctanoic acid (PFOA); zebrafish liver cells (ZFL)