title: Simultaneous detection of multiple lower genital tract pathogens by an impedimetric immunochip.
abstract: Lower genital tract infections caused by both sexually and not-sexually transmitted pathogens in women are a key public health priority worldwide, especially in developing countries. Since standard analyses are time-consuming, appropriate therapeutic intervention is often neglected or delayed. Lab-on-chips and biosensors open new perspectives and offer innovative tools to simplify the diagnosis by medical staff, especially in countries with inadequate resources. Here we report a biosensing platform based on Electrochemical Impedance Spectroscopy (EIS) that allows multiplexed detection of Candida albicans, Streptococcus agalactiae and Chlamydia trachomatis with a single biochip, enabling a quick screening thanks to the presence of different immobilized antibodies, each specific for one of the different target pathogens.
authors.affiliation: CNR Nanotechnology Institute, Lecce, Italy. Electronic address: mariaserena.chiriaco@nanotec.cnr.it.
authors.forename: Maria Serena
authors.initials: MS
authors.lastname: Chiriacò
authors.affiliation: CNR Nanotechnology Institute, Lecce, Italy. Electronic address: elisabetta.primiceri@nano.cnr.it.
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Primiceri
authors.affiliation: Dipartimento Matematica e Fisica E. De Giorgi, University of Salento, Lecce, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: De Feo
authors.affiliation: Dipartimento Matematica e Fisica E. De Giorgi, University of Salento, Lecce, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Montanaro
authors.affiliation: CNR Nanotechnology Institute, Lecce, Italy; Dipartimento Matematica e Fisica E. De Giorgi, University of Salento, Lecce, Italy.
authors.forename: Anna Grazia
authors.initials: AG
authors.lastname: Monteduro
authors.affiliation: ASL, Lecce, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Tinelli
authors.affiliation: ASL, Lecce, Italy.
authors.forename: Marcella
authors.initials: M
authors.lastname: Megha
authors.affiliation: Ekuberg Pharma S.r.L., Martano, Lecce, Italy.
authors.forename: Davide
authors.initials: D
authors.lastname: Carati
authors.affiliation: CNR Nanotechnology Institute, Lecce, Italy; Dipartimento Matematica e Fisica E. De Giorgi, University of Salento, Lecce, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Maruccio
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation; D002176:Candida albicans / Q000302:isolation & purification*; D002177:Candidiasis / Q000175:diagnosis*; D002690:Chlamydia Infections / Q000175:diagnosis*; D002692:Chlamydia trachomatis / Q000302:isolation & purification*; D058266:Dielectric Spectroscopy / Q000295:instrumentation; D004867:Equipment Design; D005260:Female; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation; D056656:Lab-On-A-Chip Devices; D013290:Streptococcal Infections / Q000175:diagnosis*; D013292:Streptococcus agalactiae / Q000302:isolation & purification*; D016585:Vaginosis, Bacterial / Q000175:diagnosis
chemical_list: D055910:Antibodies, Immobilized
keywords: Candida albicans; Chlamydia trachomatis; Electrochemical Impedance Spectroscopy; Immunoassay; Immunochip; Lab-on-chip; Microfluidics; On-chip diagnosis; Streptococcus agalactiae; Vaginosis