title: [Phosphorylation of protein kinase C in cerebrospinal fluid-contacting nucleus modulates the inflammatory pain in rats].
abstract: The present study was aimed to investigate the role of cerebrospinal fluid-contacting nucleus (CSF-CN) neurons in modulation of inflammatory pain and underlying mechanism. The inflammatory pain model was made by subcutaneous injection of the complete Freund's adjuvant (CFA) into the left hind paw of rats. The phosphorylation level of PKC (p-PKC) was examined by Western blot. Thermal withdrawal latency (TWL) of the rats was measured to assess inflammatory pain. The results showed that, compared with the sham controls, the inflammatory pain model rats showed shortened TWL on day 1, 3, and 7 after CFA injection, as well as increased level of p-PKC in CSF-CN neurons at 24 h after CFA injection. The administration of GF109203X, a PKC inhibitor, into lateral ventricle decreased the level of p-PKC protein expression and increased TWL in the model rats. These results suggest that blocking the PKC pathway in CSF-CN neurons may be an effective way to reduce or eliminate the inflammatory pain.
authors.affiliation: School of Nursing, Xuzhou Medical College, Xuzhou 221004, China.|Jiangsu Province Key Laboratory of Anesthesiology, Xuzhou Medical College, Xuzhou 221004, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Zhou
authors.affiliation: Jiangsu Province Key Laboratory of Anesthesiology, Xuzhou Medical College, Xuzhou 221004, China.|Anesthesiology Department of the Second Affiliated Hospital of Anhui Medical University, Hefei 230000, China.
authors.forename: Jia-You
authors.initials: JY
authors.lastname: Wang
authors.affiliation: Jiangsu Province Key Laboratory of Anesthesiology, Xuzhou Medical College, Xuzhou 221004, China.|Department of Anesthesiology, the People's Hospital of Changzhi, Changzhi 046000, China.
authors.forename: En-Qi
authors.initials: EQ
authors.lastname: Tian
authors.affiliation: Jiangsu Province Key Laboratory of Anesthesiology, Xuzhou Medical College, Xuzhou 221004, China. licaizhang001@163.com.
authors.forename: Li-Cai
authors.initials: LC
authors.lastname: Zhang
mesh_terms: D000818:Animals; D005620:Freund's Adjuvant; D007249:Inflammation / Q000201:enzymology*; D009474:Neurons / Q000201:enzymology*; D010146:Pain / Q000201:enzymology*; D010766:Phosphorylation; D011493:Protein Kinase C / Q000134:cerebrospinal fluid* / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D005620:Freund's Adjuvant; D011493:Protein Kinase C