title: Complete genome sequence of Colocasia bobone disease-associated virus, a putative cytorhabdovirus infecting taro.
abstract: We report the first genome sequence of a Colocasia bobone disease-associated virus (CBDaV) derived from bobone-affected taro [Colocasia esculenta L. Schott] from Solomon Islands. The negative-strand RNA genome is 12,193 nt long, with six major open reading frames (ORFs) with the arrangement 3'-N-P-P3-M-G-L-5'. Typical of all rhabdoviruses, the 3' leader and 5' trailer sequences show complementarity to each other. Phylogenetic analysis indicated that CBDaV is a member of the genus Cytorhabdovirus, supporting previous reports of virus particles within the cytoplasm of bobone-infected taro cells. The availability of the CBDaV genome sequence now makes it possible to assess the role of this virus in bobone, and possibly alomae disease of taro and confirm that this sequence is that of Colocasia bobone disease virus (CBDV).
authors.affiliation: Institute of Applied Ecology, School of Applied Sciences, Auckland University of Technology, Private Bag 92006, Auckland, 1142, New Zealand. colleen.higgins@aut.ac.nz.|AUT Roche Diagnostic Laboratory, Auckland University of Technology, Private Bag 92006, Auckland, 1142, New Zealand. colleen.higgins@aut.ac.nz.
authors.forename: Colleen M
authors.initials: CM
authors.lastname: Higgins
authors.affiliation: Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St Lucia, QLD, 4072, Australia.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Bejerman
authors.affiliation: Institute of Applied Ecology, School of Applied Sciences, Auckland University of Technology, Private Bag 92006, Auckland, 1142, New Zealand.|AUT Roche Diagnostic Laboratory, Auckland University of Technology, Private Bag 92006, Auckland, 1142, New Zealand.
authors.forename: Ming
authors.initials: M
authors.lastname: Li
authors.affiliation: Centre for Tropical Crops and Biocommodities, Queensland University of Technology, GPO Box 2434, Brisbane, QLD, 4001, Australia.
authors.forename: Anthony P
authors.initials: AP
authors.lastname: James
authors.affiliation: Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St Lucia, QLD, 4072, Australia.
authors.forename: Ralf G
authors.initials: RG
authors.lastname: Dietzgen
authors.affiliation: School of Biological Sciences, The University of Auckland, Private Bag 92019, Auckland, 1142, New Zealand.
authors.forename: Michael N
authors.initials: MN
authors.lastname: Pearson
authors.affiliation: Centre for Tropical Crops and Biocommodities, Queensland University of Technology, GPO Box 2434, Brisbane, QLD, 4001, Australia.|Victorian Infectious Diseases Reference Laboratory, Doherty Institute of Infection and Immunity, 792 Elizabeth St, Melbourne, VIC, 3000, Australia.
authors.forename: Peter A
authors.initials: PA
authors.lastname: Revill
authors.affiliation: Centre for Tropical Crops and Biocommodities, Queensland University of Technology, GPO Box 2434, Brisbane, QLD, 4001, Australia.
authors.forename: Robert M
authors.initials: RM
authors.lastname: Harding
mesh_terms: D016000:Cluster Analysis; D031060:Colocasia / Q000821:virology*; D023061:Gene Order; D016679:Genome, Viral; D008542:Melanesia; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D012367:RNA, Viral / Q000235:genetics*; D012209:Rhabdoviridae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
chemical_list: D012367:RNA, Viral