title: Antimicrobial activity of Aspilia latissima (Asteraceae).
abstract: We evaluated the antimicrobial activity of Aspilia latissima - an abundant plant from the Brazilian Pantanal region - against Candida albicans, Candida parapsilosis, Candida krusei, Candida tropicalis, Pseudomonas aeruginosa, Enterococcus faecalis, Escherichia coli and Staphylococcus aureus. The crude extracts and fractions showed activity in all tested microorganisms. The chloroform fraction of the leaves and roots showed the most antimicrobial activity against S. aureus, with an MIC of 500 μg/mL. This fraction was submitted to bioautographic assays to characterize the activity of the compounds. Two bands from the leaves (L-A and L-B) and three bands from the roots (R-C, R-D and R-E) were bioactive. Within the root-derived bands, the terpene derivatives stigmasterol, kaurenoic acid and kaura-9(11), 16-dien-18-oic acid were identified. Antibiotic activity of A. latissima is reported for the first time.
authors.affiliation: Laboratório de Produtos Naturais e Espectrometria de Massas, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brazil.
authors.forename: Jeana M E
authors.initials: JM
authors.lastname: Souza
authors.affiliation: Laboratório de Pesquisa em Microbiologia, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brazil.
authors.forename: Marilene R
authors.initials: MR
authors.lastname: Chang
authors.affiliation: Laboratório de Pesquisa em Microbiologia, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brazil.
authors.forename: Daniela Z
authors.initials: DZ
authors.lastname: Brito
authors.affiliation: Laboratório de Produtos Naturais e Espectrometria de Massas, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brazil.
authors.forename: Katyuce S
authors.initials: KS
authors.lastname: Farias
authors.affiliation: Laboratório de Botânica, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brazil.
authors.forename: Geraldo A
authors.initials: GA
authors.lastname: Damasceno-Junior
authors.affiliation: Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Izabel C C
authors.initials: IC
authors.lastname: Turatti
authors.affiliation: Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Norberto P
authors.initials: NP
authors.lastname: Lopes
authors.affiliation: Coordenação do Curso de Licenciatura em Química, Universidade Tecnológica Federal do Paraná, Apucarana, PR, Brazil.
authors.forename: Edson A
authors.initials: EA
authors.lastname: Santos
authors.affiliation: Laboratório de Produtos Naturais e Espectrometria de Massas, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brazil.
authors.forename: Carlos A
authors.initials: CA
authors.lastname: Carollo
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D000935:Antifungal Agents / Q000737:chemistry / Q000494:pharmacology*; D019659:Asteraceae / Q000737:chemistry*; D001419:Bacteria / Q000187:drug effects; D001938:Brazil; D005658:Fungi / Q000187:drug effects; D008401:Gas Chromatography-Mass Spectrometry; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D010936:Plant Extracts