title: Detection of Pathogenic Biofilms with Bacterial Amyloid Targeting Fluorescent Probe, CDy11.
abstract: Bacterial biofilms are responsible for a wide range of persistent infections. In the clinic, diagnosis of biofilm-associated infections relies heavily on culturing methods, which fail to detect nonculturable bacteria. Identification of novel fluorescent probes for biofilm imaging will greatly facilitate diagnosis of pathogenic bacterial infection. Herein, we report a novel fluorescent probe, CDy11 (compound of designation yellow 11), which targets amyloid in the Pseudomonas aeruginosa biofilm matrix through a diversity oriented fluorescent library approach (DOFLA). CDy11 was further demonstrated for in vivo imaging of P. aeruginosa in implant and corneal infection mice models.
authors.affiliation: Department of Chemistry & Med Chem Program, Life Sciences Institute, National University of Singapore , 3 Science Drive 3, 117543, Singapore.|Singapore Centre on Environmental Life Science Engineering (SCELSE), Nanyang Technological University , 637551, Singapore.|Singapore Bioimaging Consortium, Agency for Science, Technology and Research , 11 Biopolis Way, # 02-02 Helios, 138667, Singapore.
authors.forename: Jun-Young
authors.initials: JY
authors.lastname: Kim
authors.affiliation: Singapore Bioimaging Consortium, Agency for Science, Technology and Research , 11 Biopolis Way, # 02-02 Helios, 138667, Singapore.
authors.forename: Srikanta
authors.initials: S
authors.lastname: Sahu
authors.affiliation: School of Biological Sciences, Nanyang Technological University , SBS-04s-43, 60 Nanyang Avenue, 637551, Singapore.
authors.forename: Yin-Hoe
authors.initials: YH
authors.lastname: Yau
authors.affiliation: Department of Chemistry & Med Chem Program, Life Sciences Institute, National University of Singapore , 3 Science Drive 3, 117543, Singapore.
authors.forename: Xu
authors.initials: X
authors.lastname: Wang
authors.affiliation: School of Biological Sciences, Nanyang Technological University , SBS-04s-43, 60 Nanyang Avenue, 637551, Singapore.
authors.forename: Susana Geifman
authors.initials: SG
authors.lastname: Shochat
authors.affiliation: Singapore Centre on Environmental Life Science Engineering (SCELSE), Nanyang Technological University , 637551, Singapore.|Center for Microbial Communities, Department of Chemistry and Bioscience, Aalborg University , Fredrik Bajers Vej 7H, 9220 Aalborg, Denmark.
authors.forename: Per Halkjær
authors.initials: PH
authors.lastname: Nielsen
authors.affiliation: Center for Microbial Communities, Department of Chemistry and Bioscience, Aalborg University , Fredrik Bajers Vej 7H, 9220 Aalborg, Denmark.
authors.forename: Morten Simonsen
authors.initials: MS
authors.lastname: Dueholm
authors.affiliation: Interdisciplinary Nanoscience Center (iNANO), Department of Molecular Biology and Genetics, Aarhus University , 8000 Aarhus C, Denmark.
authors.forename: Daniel E
authors.initials: DE
authors.lastname: Otzen
authors.affiliation: Department of Chemistry & Med Chem Program, Life Sciences Institute, National University of Singapore , 3 Science Drive 3, 117543, Singapore.
authors.forename: Jungyeol
authors.initials: J
authors.lastname: Lee
authors.affiliation: Singapore Centre on Environmental Life Science Engineering (SCELSE), Nanyang Technological University , 637551, Singapore.
authors.forename: May Margarette Salido
authors.initials: MM
authors.lastname: Delos Santos
authors.affiliation: Singapore Centre on Environmental Life Science Engineering (SCELSE), Nanyang Technological University , 637551, Singapore.|Interdisciplinary Graduate School, Nanyang Technological University , 637551, Singapore.
authors.forename: Joey Kuok Hoong
authors.initials: JK
authors.lastname: Yam
authors.affiliation: Singapore Bioimaging Consortium, Agency for Science, Technology and Research , 11 Biopolis Way, # 02-02 Helios, 138667, Singapore.
authors.forename: Nam-Young
authors.initials: NY
authors.lastname: Kang
authors.affiliation: Singapore Bioimaging Consortium, Agency for Science, Technology and Research , 11 Biopolis Way, # 02-02 Helios, 138667, Singapore.
authors.forename: Sung-Jin
authors.initials: SJ
authors.lastname: Park
authors.affiliation: Department of Chemistry & Med Chem Program, Life Sciences Institute, National University of Singapore , 3 Science Drive 3, 117543, Singapore.|Singapore Centre on Environmental Life Science Engineering (SCELSE), Nanyang Technological University , 637551, Singapore.
authors.forename: Hawyoung
authors.initials: H
authors.lastname: Kwon
authors.affiliation: Singapore Centre on Environmental Life Science Engineering (SCELSE), Nanyang Technological University , 637551, Singapore.
authors.forename: Thomas
authors.initials: T
authors.lastname: Seviour
authors.affiliation: Singapore Centre on Environmental Life Science Engineering (SCELSE), Nanyang Technological University , 637551, Singapore.|School of Biological Sciences, Nanyang Technological University , SBS-04s-43, 60 Nanyang Avenue, 637551, Singapore.
authors.forename: Liang
authors.initials: L
authors.lastname: Yang
authors.affiliation: Singapore Centre on Environmental Life Science Engineering (SCELSE), Nanyang Technological University , 637551, Singapore.|Costerton Biofilm Center, Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen , Blegdamsvej 3B, DK-2200 Copenhagen, Denmark.
authors.forename: Michael
authors.initials: M
authors.lastname: Givskov
authors.affiliation: Department of Chemistry & Med Chem Program, Life Sciences Institute, National University of Singapore , 3 Science Drive 3, 117543, Singapore.|Singapore Bioimaging Consortium, Agency for Science, Technology and Research , 11 Biopolis Way, # 02-02 Helios, 138667, Singapore.
authors.forename: Young-Tae
authors.initials: YT
authors.lastname: Chang
mesh_terms: D000682:Amyloid / Q000737:chemistry*; D018441:Biofilms; D005456:Fluorescent Dyes; D011550:Pseudomonas aeruginosa / Q000737:chemistry
chemical_list: D000682:Amyloid; D005456:Fluorescent Dyes