title: Identification of cardiac-related circulating microRNA profile in human chronic heart failure.
abstract: BACKGROUND
  During chronic heart failure, levels of circulating miRNAs endued with characteristics of diseased cells could be identified as biomarkers. In this study, we sought to identify cardiac-related circulating miRNAs as biomarkers of failing heart.
  
  
  METHODS
  Total RNA of plasma and heart samples was extracted from 10 normal controls and 14 patients with chronic heart failure. Microarray was applied for miRNA profiles. Validation and organ/tissue distribution analysis was performed by qRT-PCR. In addition, bioinformatics analysis was performed to understand the critical roles of these cardiac-related circulating miRNAs in heart failure.
  
  
  RESULTS
  Results showed that levels of more than half of the miRNAs dysregulated in heart failed to show any differences in plasma. Meanwhile, more than 90% of the miRNAs dysregulated in plasma remained stable in heart. Four cardiac fibroblast-derived miRNAs (miR-660-3p, miR-665, miR-1285-3p and miR-4491) were found significantly upregulated in heart and plasma during heart failure. These 4 miRNAs strongly discriminated patients from controls, and 3 of them showed significant correlations with LVEF.
  
  
  CONCLUSIONS
  This study provides global profiles of miRNAs changes in plasma and failing heart, and using a circulation-tissue miRNA profiling comparison model, we successfully identify 3 cardiac-related circulating miRNAs as potential biomarkers for diagnosis of heart failure.
authors.affiliation: Departments of Internal Medicine and The Institute of Hypertension Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People's Rep. of China.
authors.forename: Huaping
authors.initials: H
authors.lastname: Li
authors.affiliation: Departments of Internal Medicine and The Institute of Hypertension Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People's Rep. of China.
authors.forename: Jiahui
authors.initials: J
authors.lastname: Fan
authors.affiliation: Departments of Internal Medicine and The Institute of Hypertension Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People's Rep. of China.
authors.forename: Zhongwei
authors.initials: Z
authors.lastname: Yin
authors.affiliation: Departments of Internal Medicine and The Institute of Hypertension Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People's Rep. of China.
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.affiliation: Departments of Internal Medicine and The Institute of Hypertension Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People's Rep. of China.
authors.forename: Chen
authors.initials: C
authors.lastname: Chen
authors.affiliation: Departments of Internal Medicine and The Institute of Hypertension Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People's Rep. of China.
authors.forename: Dao Wen
authors.initials: DW
authors.lastname: Wang
mesh_terms: D000328:Adult; D000368:Aged; D002478:Cells, Cultured; D002908:Chronic Disease; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D020869:Gene Expression Profiling / Q000379:methods*; D006333:Heart Failure / Q000097:blood / Q000235:genetics*; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood / Q000235:genetics*; D008875:Middle Aged; D009206:Myocardium / Q000378:metabolism*; D032383:Myocytes, Cardiac / Q000166:cytology / Q000378:metabolism; D009928:Organ Specificity / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055815:Young Adult
chemical_list: C000611896:MIRN-4491 microRNA, human; C572554:MIRN1285 microRNA, human; C577172:MIRN660 microRNA, human; C000592932:MIRN665 microRNA, human; D035683:MicroRNAs
keywords: Gerotarget; circulating; heart failure; miRNA profile; tissue