title: Fractionation and evaluation of proteins in roots of Echinacea purpurea (L.) Moench.
abstract: Echinacea purpurea (L.) Moench, a member of the Asteraceae family, is a plant rich in flavonoids, essential oils, phenolic compounds, saponins, polysaccharides and glycoproteins. The aim of the study was to evaluate the protein content in dried roots of Echinacea purpurea (L.) Moench after homogenization of roots with liquid nitrogen, extraction in 0.01 mol L-1 phosphate-buffered saline (PBS) and purification followed by fractionation of proteins using gel filtration chromatography. Total concentration of proteins was measured using the Bradford method, and evaluation of the molecular mass of proteins was accomplished by applying the SDS-PAGE gel electrophoresis. The Bradford assay revealed that the highest concentration of proteins in fractions collected after gel filtration chomatography was 4.66-6.07 mg mL-1. Glycoproteins, alkamides and polysaccharides in roots of Echinacea purpurea (L.) Moench are chemical compounds that are responsible for their immunomodulatory properties. However, information about the difference of protein contents in fresh and dried roots of E. purpurea is insufficient.
authors.forename: Gabriele
authors.initials: G
authors.lastname: Balciunaite
authors.forename: Jovita
authors.initials: J
authors.lastname: Juodsnukyte
authors.forename: Arunas
authors.initials: A
authors.lastname: Savickas
authors.forename: Ona
authors.initials: O
authors.lastname: Ragazinskiene
authors.forename: Luka
authors.initials: L
authors.lastname: Siatkute
authors.forename: Gitana
authors.initials: G
authors.lastname: Zvirblyte
authors.forename: Edita
authors.initials: E
authors.lastname: Mistiniene
authors.forename: Nijole
authors.initials: N
authors.lastname: Savickiene
mesh_terms: D005591:Chemical Fractionation; D002850:Chromatography, Gel; D020900:Echinacea / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D008970:Molecular Weight; D010940:Plant Proteins / Q000032:analysis*; D018517:Plant Roots / Q000737:chemistry*; D040901:Proteomics / Q000379:methods
chemical_list: D010940:Plant Proteins