title: MicroRNA-185 inhibits angiogenesis in human microvascular endothelial cells through targeting stromal interaction molecule 1.
abstract: Angiogenesis is a vital biological mechanism representing the adaptive response to a variety of pathological stimuli such as hypoxia. It is regulated by several pro-angiogenic and anti-angiogenic microRNAs. Studies have demonstrated an altered microRNA-185 (miR-185) expression in endothelial cells under hypoxic conditions; however, its role in angiogenesis has not been elucidated. We investigated the role of miR-185 in angiogenesis and found that miR-185 had an inhibitory effect on cell proliferation, migration, and tube formation. Stromal interaction molecule 1 (STIM1) appeared to be a direct target of miR-185 by computational prediction; this was confirmed by luciferase reporter assay. Silencing of the STIM1 gene was found to mimic miR-185-mediated inhibition of angiogenesis. STIM1 overexpression eliminated the anti-angiogenic effect of miR-185. Our study results suggest a direct interaction between miR-185 and STIM1 mRNA in microvascular endothelial cells. MicroRNA-185 acted as a negative regulator of angiogenesis in microvascular endothelial cells through downregulation of the STIM1 protein.
authors.affiliation: Tongji University School of Medicine, Shanghai, 200092, China.
authors.forename: Jiayin
authors.initials: J
authors.lastname: Hou
authors.affiliation: Department of Cardiovascular Medicine, Pudong New Area Zhoupu Hospital, Shanghai, 201318, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Cardiovascular Medicine, Pudong New Area Zhoupu Hospital, Shanghai, 201318, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhu
authors.affiliation: Department of Cardiovascular Medicine, Pudong New Area Zhoupu Hospital, Shanghai, 201318, China.
authors.forename: Yingbiao
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Cardiovascular Medicine, Pudong New Area Zhoupu Hospital, Shanghai, 201318, China.
authors.forename: Bei
authors.initials: B
authors.lastname: Tian
authors.affiliation: Department of Cardiovascular Medicine, Pudong New Area Zhoupu Hospital, Shanghai, 201318, China.
authors.forename: Li
authors.initials: L
authors.lastname: Cui
authors.affiliation: Department of Cardiovascular Medicine, Pudong New Area Zhoupu Hospital, Shanghai, 201318, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Tongji University School of Medicine, Shanghai, 200092, China.|Department of Cardiovascular Medicine, Pudong New Area Zhoupu Hospital, Shanghai, 201318, China.
authors.forename: Xinming
authors.initials: X
authors.lastname: Li
mesh_terms: D020413:3' Untranslated Regions; D001483:Base Sequence; D002460:Cell Line; D002465:Cell Movement; D049109:Cell Proliferation; D015536:Down-Regulation; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism; D006801:Humans; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D018919:Neovascularization, Physiologic / Q000502:physiology*; D016376:Oligonucleotides, Antisense / Q000378:metabolism; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D016415:Sequence Alignment; D000071737:Stromal Interaction Molecule 1 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*
chemical_list: D020413:3' Untranslated Regions; D035683:MicroRNAs; D009363:Neoplasm Proteins; D016376:Oligonucleotides, Antisense; D034741:RNA, Small Interfering; C103530:STIM1 protein, human; D000071737:Stromal Interaction Molecule 1
keywords: angiogenesis; human microvascular endothelial cells; microRNA-185; stromal interaction molecule 1