title: Study on the Association Between miRNA-202 Expression and Drug Sensitivity in Multiple Myeloma Cells.
abstract: An increasing amount of experimental evidence has shown that miRNAs play a causal role in hematologic tumorigenesis. In this study, we characterized the role of miR-202 in multiple myeloma (MM) drug sensitivity. The potential binding site of miR-202 and B cell-activating factor (BAFF) was confirmed by luciferase reporter assay. MM cells were transfected with miR-202 mimics and inhibitor. Cells growth was measured by WST-1 cell proliferation assay and Annexin V-FLUOS apoptosis assay. BAFF and miR-202 mRNA levels were measured by real-time PCR. Meanwhile, BAFF, Bcl-2 family survival proteins and MAPK pathway proteins were measured by Western blot. It was found that miR-202 was functioned as a modulator of BAFF expression. miR-202 over-expression sensitized MM cells to bortezomib (Bort) but less to Thalidomide (Thal) and dexamethasone (Dex). miR-202 mimics in combination with Bort inhibited MM cell survival more effectively as compared with Bort treatment alone. Our study also provided experimental evidence that JNK/SAPK signaling pathway was involved in the regulatory effect of miR-202 on drug resistance of MM cells. These results suggest that the regulatory mechanism of miR-202 expression may be a promising target for fine-tuning anti-myeloma therapy.
authors.affiliation: Surgical Comprehensive Laboratory, Affiliated Hospital of Nantong University, #20 Xisi Road, Nantong, 226001, JS, People's Republic of China.
authors.forename: Xianjuan
authors.initials: X
authors.lastname: Shen
authors.affiliation: Nantong University, #9 Seyuan Road, Nantong, 226001, JS, People's Republic of China.
authors.forename: Yuehua
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Surgical Comprehensive Laboratory, Affiliated Hospital of Nantong University, #20 Xisi Road, Nantong, 226001, JS, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Qi
authors.affiliation: Surgical Comprehensive Laboratory, Affiliated Hospital of Nantong University, #20 Xisi Road, Nantong, 226001, JS, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Shi
authors.affiliation: Surgical Comprehensive Laboratory, Affiliated Hospital of Nantong University, #20 Xisi Road, Nantong, 226001, JS, People's Republic of China.
authors.forename: Xinhua
authors.initials: X
authors.lastname: Wu
authors.affiliation: Laboratory Medicine Center, Affiliated Hospital of Nantong University, #20 Xisi Road, Nantong, 226001, JS, People's Republic of China.
authors.forename: Hongbing
authors.initials: H
authors.lastname: Ni
authors.affiliation: Surgical Comprehensive Laboratory, Affiliated Hospital of Nantong University, #20 Xisi Road, Nantong, 226001, JS, People's Republic of China. jsq814@hotmail.com.|Nantong University, #9 Seyuan Road, Nantong, 226001, JS, People's Republic of China. jsq814@hotmail.com.|Laboratory Medicine Center, Affiliated Hospital of Nantong University, #20 Xisi Road, Nantong, 226001, JS, People's Republic of China. jsq814@hotmail.com.
authors.forename: Shaoqing
authors.initials: S
authors.lastname: Ju
mesh_terms: D000368:Aged; D017209:Apoptosis / Q000235:genetics; D051017:Apoptosis Regulatory Proteins / Q000235:genetics; D053264:B-Cell Activating Factor / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D003907:Dexamethasone / Q000494:pharmacology; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006801:Humans; D048670:MAP Kinase Kinase 4 / Q000235:genetics; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics; D009101:Multiple Myeloma / Q000188:drug therapy / Q000235:genetics*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D015398:Signal Transduction; D013792:Thalidomide / Q000494:pharmacology
chemical_list: D051017:Apoptosis Regulatory Proteins; D053264:B-Cell Activating Factor; D035683:MicroRNAs; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; C505719:TNFSF13B protein, human; D013792:Thalidomide; D003907:Dexamethasone; D020928:Mitogen-Activated Protein Kinases; D048670:MAP Kinase Kinase 4
keywords: B cell-activating factor (BAFF); Drug resistance; Multiple myeloma (MM); Signaling pathway; microRNA