title: Systematic analysis of key miRNAs and related signaling pathways in colorectal tumorigenesis.
abstract: The development of colorectal cancers (CRC) is accompanied with the acquisition and maintenance of specific genomic alterations. These alterations can emerge in premalignant adenomas and faithfully maintained in highly advanced tumors. miRNAs are a class of small non-coding RNAs that are frequently deregulated in human cancers and negatively regulate a wide variety of protein coding genes. To identify the sequential alterations of miRNAs and its regulatory networks during CRC development and progression, we detected the miRNA expression profiles of tissue samples from normal colon, colorectal adenoma and CRC using miRNA microarray. qRT-PCR assay was used to validate and select the miRNAs with differential expression among the three groups, and the computer-aided algorithms of TargetScan, miRanda, miRwalk, RNAhybrid and PicTar were used to search for the possible targets of the selected 8 miRNAs (miR-18a, miR-18b, miR-31, miR-142-5p, miR-145, miR-212, miR-451, and miR-638) with continuous alterated expression. These potential target genes were enriched in several key signal transduction pathways (KEGG pathway analysis), which have been proved to be closely related to colorectal tumorigenesis. To confirm the reliability of the analyses, we identified that the metastasis-related gene ZO-1 is a certain target of miR-212 in CRC and keeps declining during CRC progression. By following these analyses, we might gain an in-depth understanding of the molecular regulatory networks of colorectal tumorigenesis and provide new potential targets for the diagnostic and therapeutic interventions of this disease.
authors.affiliation: Wuxi Oncology Institute, Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu 214062, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yin
authors.affiliation: Wuxi Oncology Institute, Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu 214062, China.
authors.forename: Mingxu
authors.initials: M
authors.lastname: Song
authors.affiliation: Department of Laboratory Medicine, Affiliated Hospital of Xuzhou Medical College, Xuzhou, Jiangsu 221002, China; Medical Technology Institute of Xuzhou Medical College, Xuzhou, Jiangsu 221002, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Gu
authors.affiliation: Department of Pathology, Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu 214062, China.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Qi
authors.affiliation: Wuxi Oncology Institute, Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu 214062, China.
authors.forename: Yaling
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Wuxi Oncology Institute, Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu 214062, China.
authors.forename: Yuyang
authors.initials: Y
authors.lastname: Feng
authors.affiliation: Wuxi Oncology Institute, Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu 214062, China.
authors.forename: Heyong
authors.initials: H
authors.lastname: Liu
authors.affiliation: Wuxi Oncology Institute, Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu 214062, China.
authors.forename: Leyuan
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Wuxi Oncology Institute, Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu 214062, China.
authors.forename: Zehua
authors.initials: Z
authors.lastname: Bian
authors.affiliation: Wuxi Oncology Institute, Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu 214062, China.
authors.forename: Jiwei
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Institute of Dermatology and Department of Dermatology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, China.
authors.forename: Xianbo
authors.initials: X
authors.lastname: Zuo
authors.affiliation: Wuxi Oncology Institute, Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu 214062, China. Electronic address: hzhwxsy@126.com.
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Huang
mesh_terms: D063646:Carcinogenesis / Q000235:genetics*; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D053263:Gene Regulatory Networks / Q000235:genetics; D006801:Humans; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D062826:Zonula Occludens-1 Protein / Q000096:biosynthesis / Q000235:genetics*
chemical_list: C551411:MIRN212 microRNA, human; D035683:MicroRNAs; C569042:TJP1 protein, human; D062826:Zonula Occludens-1 Protein
keywords: Bioinformatic analysis; Colorectal cancer; KEGG pathway analysis; MicroRNA; Regulatory networks