title: Eomesodermin(lo) CTLA4(hi) Alloreactive CD8+ Memory T Cells Are Associated With Prolonged Renal Transplant Survival Induced by Regulatory Dendritic Cell Infusion in CTLA4 Immunoglobulin-Treated Nonhuman Primates.
abstract: BACKGROUND
  Memory T cells (Tmem), particularly those resistant to costimulation blockade (CB), are a major barrier to transplant tolerance. The transcription factor Eomesodermin (Eomes) is critical for Tmem development and maintenance, but its expression by alloactivated T cells has not been examined in nonhuman primates.
  
  
  METHODS
  We evaluated Eomes and coinhibitory cytotoxic T lymphocyte antigen-4 (CTLA4) expression by alloactivated rhesus monkey T cells in the presence of CTLA4 immunoglobulin, both in vitro and in renal allograft recipients treated with CTLA4Ig, with or without regulatory dendritic cell (DCreg) infusion.
  
  
  RESULTS
  In normal monkeys, CD8+ T cells expressed significantly more Eomes than CD4+ T cells. By contrast, CD8+ T cells displayed minimal CTLA4. Among T cell subsets, central Tmem (Tcm) expressed the highest levels of Eomes. Notably, Eomes(lo)CTLA4(hi) cells displayed higher levels of CD25 and Foxp3 than Eomes(hi)CTLA4(lo) CD8+ T cells. After allostimulation, distinct proliferating Eomes(lo)CTLA4(hi) and Eomes(hi)CTLA4(lo) CD8+ T cell populations were identified, with a high proportion of Tcm being Eomes(lo)CTLA4(hi). CB with CTLA4Ig during allostimulation of CD8+ T cells reduced CTLA4 but not Eomes expression, significantly reducing Eomes(lo)CTLA4(hi) cells. After transplantation with CB and rapamycin, donor-reactive Eomes(lo)CTLA4(hi) CD8+ T cells were reduced. However, in monkeys also given DCreg, absolute numbers of these cells were elevated significantly.
  
  
  CONCLUSIONS
  Low Eomes and high CTLA4 expression by donor-reactive CD8+ Tmem is associated with prolonged renal allograft survival induced by DCreg infusion in CTLA4Ig-treated monkeys. Prolonged allograft survival associated with DCreg infusion may be related to maintenance of donor-reactive Eomes(lo)CTLA4(hi) Tcm.
authors.affiliation: 1 Starzl Transplantation Institute, Department of Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA.2 Department of Immunology, University of Pittsburgh School of Medicine, Pittsburgh, PA.
authors.forename: Mohamed B
authors.initials: MB
authors.lastname: Ezzelarab
authors.forename: Lien
authors.initials: L
authors.lastname: Lu
authors.forename: Hao
authors.initials: H
authors.lastname: Guo
authors.forename: Alan F
authors.initials: AF
authors.lastname: Zahorchak
authors.forename: William F
authors.initials: WF
authors.lastname: Shufesky
authors.forename: David K C
authors.initials: DK
authors.lastname: Cooper
authors.forename: Adrian E
authors.initials: AE
authors.lastname: Morelli
authors.forename: Angus W
authors.initials: AW
authors.lastname: Thomson
mesh_terms: D000069594:Abatacept / Q000494:pharmacology*; D064591:Allografts; D000818:Animals; D018106:CD28 Antigens / Q000276:immunology / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D060908:CTLA-4 Antigen / Q000276:immunology / Q000378:metabolism*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism / Q000637:transplantation*; D006085:Graft Survival / Q000187:drug effects*; D007156:Immunologic Memory / Q000187:drug effects*; D016130:Immunophenotyping; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D016030:Kidney Transplantation / Q000009:adverse effects; D008213:Lymphocyte Activation / Q000187:drug effects*; D008253:Macaca mulatta; D008297:Male; D010641:Phenotype; D061026:Programmed Cell Death 1 Receptor / Q000276:immunology / Q000378:metabolism; D020123:Sirolimus / Q000494:pharmacology; D020825:T-Box Domain Proteins / Q000276:immunology / Q000378:metabolism*; D013997:Time Factors
chemical_list: D018106:CD28 Antigens; D060908:CTLA-4 Antigen; D007166:Immunosuppressive Agents; D061026:Programmed Cell Death 1 Receptor; D020825:T-Box Domain Proteins; D000069594:Abatacept; D020123:Sirolimus