title: Chemo/Photoacoustic Dual Therapy with mRNA-Triggered DOX Release and Photoinduced Shockwave Based on a DNA-Gold Nanoplatform.
abstract: A multifunctional nanoparticle based on gold nanorod (GNR), utilizing mRNA triggered chemo-drug release and near-infrared photoacoustic effect, is developed for a combined chemo-photoacoustic therapy. The constructed nanoparticle (GNR-DNA/FA:DOX) comprises three functional components: (i) GNR as the drug delivery platform and photoacoustic effect enhancer; (ii) toehold-possessed DNA dressed on the GNR to load doxorubicin (DOX) to implement a tumor cell specific chemotherapy; and (iii) folate acid (FA) modified on GNR to guide the nanoparticle to target tumor cells. The results show that, upon an effective and specific delivery of the nanoparticles to the tumor cells with overexpressed folate receptors, the cytotoxic DOX loaded on the GNR-DNA nanoplatform can be released through DNA displacement reaction in melanoma-associated antigen gene mRNA expressed cells. With 808 nm pulse laser irradiation, the photoacoustic effect of the GNR leads to a direct physical damage to the cells. The combined treatment of the two modalities can effectively destroy tumor cells and eradicate the tumors with two distinctively different and supplementing mechanisms. With the nanoparticle, photoacoustic imaging is successfully performed in situ to monitor the drug distribution and tumor morphology for therapeutical guidance. With further in-depth investigation, the proposed nanoparticle may provide an effective and safe alternative cancer treatment modality.
authors.affiliation: MOE Key Laboratory of Laser Life Science and Institute of Laser Life Science, College of Biophotonics South China Normal University, Guangzhou, 510631, P. R. China.
authors.forename: Yundong
authors.initials: Y
authors.lastname: Zang
authors.affiliation: MOE Key Laboratory of Laser Life Science and Institute of Laser Life Science, College of Biophotonics South China Normal University, Guangzhou, 510631, P. R. China.
authors.forename: Yanchun
authors.initials: Y
authors.lastname: Wei
authors.affiliation: MOE Key Laboratory of Laser Life Science and Institute of Laser Life Science, College of Biophotonics South China Normal University, Guangzhou, 510631, P. R. China.
authors.forename: Yujiao
authors.initials: Y
authors.lastname: Shi
authors.affiliation: MOE Key Laboratory of Laser Life Science and Institute of Laser Life Science, College of Biophotonics South China Normal University, Guangzhou, 510631, P. R. China.
authors.forename: Qun
authors.initials: Q
authors.lastname: Chen
authors.affiliation: MOE Key Laboratory of Laser Life Science and Institute of Laser Life Science, College of Biophotonics South China Normal University, Guangzhou, 510631, P. R. China.|Joint Laboratory of Laser Oncology with Cancer Center of Sun Yat-sen University, South China Normal University, Guangzhou, 510631, China.
authors.forename: Da
authors.initials: D
authors.lastname: Xing
mesh_terms: D000818:Animals; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000737:chemistry*; D004317:Doxorubicin / Q000493:pharmacokinetics / Q000494:pharmacology / Q000627:therapeutic use*; D065546:Drug Liberation; D000067493:Dynamic Light Scattering; D004705:Endocytosis / Q000187:drug effects; D005260:Female; D005492:Folic Acid / Q000494:pharmacology; D006046:Gold / Q000737:chemistry*; D020128:Inhibitory Concentration 50; D053768:Metal Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D008807:Mice, Inbred BALB C; D043942:Nanotubes / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D061088:Photoacoustic Techniques; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D055672:Static Electricity; D014018:Tissue Distribution / Q000187:drug effects; D014465:Ultrasonics
chemical_list: D012333:RNA, Messenger; D006046:Gold; D004317:Doxorubicin; D004247:DNA; D005492:Folic Acid
keywords: cancer therapy; doxorubicin; gold nanorods; mRNA; nanoplatforms; photoacoustic effect