title: Prognostic value of miR-141 downregulation in gastric cancer.
abstract: Previous research has shown that microRNA-141 (miR-141) expression levels are associated with survival in several types of cancer. In the present study, we investigated the clinical significance and prognostic value of miR-141 in gastric cancer. Paired tissue specimens (tumor and adjacent normal mucosa) from 95 patients with gastric cancer were obtained at the Department of General Surgery, Xiangya Hospital, Central South University from March 2009 to February 2014. The levels of miR-141 in cancerous and corresponding non-cancerous tissues were detected by quantitative reverse transcription-polymerase chain reaction. Associations between clinicopathological parameters and miR-141 expression were evaluated using chi-square tests. Overall survival was calculated and survival curves were plotted using the Kaplan-Meier method; differences between groups were compared using log-rank tests. Compared to the matched normal gastric mucosa, gastric cancer tissues had significantly lower miR-141 expression levels (P < 0.001). This decreased miR-141 expression was significantly associated with tumor differentiation (P = 0.044), positive lymph node metastasis (P = 0.010), distant metastasis (P < 0.001), and advanced tumor-node-metastasis (TNM) stage (P < 0.001). Furthermore, a significant relationship was found between miR-141 expression and overall survival (P = 0.012, log-rank test). Cox regression analysis revealed that lymph node metastasis (P = 0.003), distant metastasis (P = 0.001), TNM stage (P < 0.001), and miR- 141 expression (P = 0.007) were independent prognostic factors in patients with gastric cancer. Our data provide evidence that the downregulation of miR-141 may contribute to the aggressive progression and poor prognosis of human gastric cancer.
authors.affiliation: Department of General Surgery, Xiangya Hospital, Central South University, Changsha, Hunan Province, China.
authors.forename: Y B
authors.initials: YB
authors.lastname: Lu
authors.affiliation: Department of General Surgery, Xiangya Hospital, Central South University, Changsha, Hunan Province, China.
authors.forename: J J
authors.initials: JJ
authors.lastname: Hu
authors.affiliation: Department of General Surgery, Xiangya Hospital, Central South University, Changsha, Hunan Province, China.
authors.forename: W J
authors.initials: WJ
authors.lastname: Sun
authors.affiliation: Department of General Surgery, Xiangya Hospital, Central South University, Changsha, Hunan Province, China.
authors.forename: X H
authors.initials: XH
authors.lastname: Duan
authors.affiliation: Department of General Surgery, Xiangya Hospital, Central South University, Changsha, Hunan Province, China.
authors.forename: X
authors.initials: X
authors.lastname: Chen
mesh_terms: D000328:Adult; D000368:Aged; D018450:Disease Progression; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D060787:Neoplasm Grading; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging; D011379:Prognosis; D016016:Proportional Hazards Models; D013274:Stomach Neoplasms / Q000235:genetics* / Q000401:mortality* / Q000473:pathology
chemical_list: C533895:MIRN141 microRNA, human; D035683:MicroRNAs