title: Identification of miR-15b as a transformation-related factor in mantle cell lymphoma.
abstract: Mantle cell lymphoma (MCL) is an aggressive B cell lymphoma with a poor prognosis. It is characterized by the t(11;14)(q13;q32) translocation, resulting in over-expression of CCND1. Morphologically, MCL is categorised into two types: classical MCL (cMCL) and aggressive MCL (aMCL), with a proportion of cMCL progressing to develop into aMCL. miRNAs are currently considered to be important regulators for cell behavior and are deregulated in many malignancies. Although several genetic alterations have been implicated in the transformation of cMCL to aMCL, the involvement of miRNAs in transformation is not known. In an effort to identify the miRNAs related to the transformation of MCL, miRNA microarray analyses were used for cMCL and aMCL cases. These analyses demonstrated significant differences in the expression of seven microRNAs based on a t-test (p-value <0.05); miR-15b was greatly upregulated in aMCL. Locked nucleic acid in situ hybridization showed increased staining of miR-15b in formalin-fixed paraffin-embedded sections of aMCL. These results correlated well with the microRNA microarray analysis. Although the molecular functions of miR-15b are largely unknown, it has been found to be associated with the cell cycle and apoptosis. However, the physiological significance of increased miR-15b in MCL is still unknown. Our present findings suggest that the upregulated expression of miR-15b is likely to play an important role in the trans-formation of cMCL to aMCL.
authors.affiliation: Department of Pathology, School of Medicine, Kurume University, Kurume, Japan.
authors.forename: Fumiko
authors.initials: F
authors.lastname: Arakawa
authors.affiliation: Department of Pathology, School of Medicine, Kurume University, Kurume, Japan.
authors.forename: Yoshizo
authors.initials: Y
authors.lastname: Kimura
authors.affiliation: Department of Pathology, School of Medicine, Kurume University, Kurume, Japan.
authors.forename: Noriaki
authors.initials: N
authors.lastname: Yoshida
authors.affiliation: Department of Pathology, School of Medicine, Kurume University, Kurume, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Miyoshi
authors.affiliation: Cell Innovator Inc., Venture Business Laboratory of Kyushu University, Fukuoka, Japan.
authors.forename: Atushi
authors.initials: A
authors.lastname: Doi
authors.affiliation: Cell Innovator Inc., Venture Business Laboratory of Kyushu University, Fukuoka, Japan.
authors.forename: Kaori
authors.initials: K
authors.lastname: Yasuda
authors.affiliation: Department of Pathology, School of Medicine, Kurume University, Kurume, Japan.
authors.forename: Kazutaka
authors.initials: K
authors.lastname: Nakajima
authors.affiliation: Department of Pathology, School of Medicine, Kurume University, Kurume, Japan.
authors.forename: Junichi
authors.initials: J
authors.lastname: Kiyasu
authors.affiliation: Department of Pathology, School of Medicine, Kurume University, Kurume, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Niino
authors.affiliation: Department of Pathology, School of Medicine, Kurume University, Kurume, Japan.
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Sugita
authors.affiliation: Laboratory of Molecular Gene Technology and Molecular Biosciences, Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Fukuoka, Japan.
authors.forename: Kosuke
authors.initials: K
authors.lastname: Tashiro
authors.affiliation: Laboratory of Molecular Gene Technology and Molecular Biosciences, Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Fukuoka, Japan.
authors.forename: Satoru
authors.initials: S
authors.lastname: Kuhara
authors.affiliation: Department of Pathology, School of Medicine, Kurume University, Kurume, Japan.
authors.forename: Masao
authors.initials: M
authors.lastname: Seto
authors.affiliation: Department of Pathology, School of Medicine, Kurume University, Kurume, Japan.
authors.forename: Koichi
authors.initials: K
authors.lastname: Ohshima
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D017209:Apoptosis / Q000235:genetics; D014408:Biomarkers, Tumor / Q000235:genetics; D002453:Cell Cycle / Q000235:genetics; D019938:Cyclin D1 / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006801:Humans; D016393:Lymphoma, B-Cell / Q000235:genetics; D020522:Lymphoma, Mantle-Cell / Q000235:genetics*; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D046228:Microarray Analysis / Q000379:methods; D008875:Middle Aged; D011379:Prognosis; D014170:Transformation, Genetic / Q000235:genetics*; D015854:Up-Regulation / Q000235:genetics
chemical_list: D014408:Biomarkers, Tumor; C530104:CCND1 protein, human; C505361:MIRN15 microRNA, human; D035683:MicroRNAs; D019938:Cyclin D1