title: A novel electrochemical aptasensor for bisphenol A assay based on triple-signaling strategy.
abstract: Based on a triple-signaling strategy, a novel electrochemical aptasensor has been developed for sensitive and selective detection of bisphenol A (BPA). The thiolated ferrocene (Fc)-modified BPA-binding aptamer probe (Fc-P) was immobilized on the gold electrode and then hybridized with the methylene blue (MB)-modified complementary DNA probe (MB-P) to form a rigid double-stranded DNA (ds-DNA). The specific interaction between BPA and Fc-P led to the release of MB-P from the sensing interface and the conformational change of Fc-P. As a result, the oxidation peak currents of Fc and BPA increased with the increase of the concentration of target (BPA) according to the "signal-on" mode while that of MB decreased with the increase of the BPA concentration according to the "signal-off" mode. By superimposing the triple signal changes, BPA was detected sensitively with a linear range from 1 pM to 100 pM. The detection limit is 0.19 pM, and much lower than that obtained by most of the reported electrochemical methods. The aptasensor also exhibited satisfactory specificity, selectivity, reproducibility and stability. By changing the specific aptamers, this strategy could be easily extended to detect other redox targets, showing promising applications in environmental analysis, food safety monitoring, and bioanalysis.
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China.
authors.forename: Peng
authors.initials: P
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China.
authors.forename: Yunqing
authors.initials: Y
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China. Electronic address: mickyxie@hnu.edu.cn.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China.
authors.forename: Jiawan
authors.initials: J
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China.
authors.forename: Erhu
authors.initials: E
authors.lastname: Xiong
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Li
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China. Electronic address: chenjinhua@hnu.edu.cn.
authors.forename: Jinhua
authors.initials: J
authors.lastname: Chen
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D001559:Benzhydryl Compounds / Q000032:analysis*; D015374:Biosensing Techniques / Q000379:methods*; D060766:Drinking Water / Q000032:analysis; D055664:Electrochemical Techniques / Q000379:methods*; D005296:Ferrous Compounds / Q000737:chemistry*; D057230:Limit of Detection; D000075163:Metallocenes; D008751:Methylene Blue / Q000737:chemistry*; D010636:Phenols / Q000032:analysis*; D014874:Water Pollutants, Chemical / Q000032:analysis*; D000098826:Bisphenol A Compounds
chemical_list: D052157:Aptamers, Nucleotide; D001559:Benzhydryl Compounds; D060766:Drinking Water; D005296:Ferrous Compounds; D000075163:Metallocenes; D008751:Methylene Blue; D010636:Phenols; D014874:Water Pollutants, Chemical; C006780:bisphenol A; D000098826:Bisphenol A Compounds; C004998:ferrocene
keywords: Bisphenol A; Electrochemical aptasensor; Ferrocene; Methylene blue; Triple-signaling