title: Gordonia didemni sp. nov. an actinomycete isolated from the marine ascidium Didemnum sp.
abstract: A novel actinobacterium, designated isolate B204(T), was isolated from a marine ascidian Didemnum sp., collected from São Paulo, Brazil, and its taxonomic position established using data from a polyphasic study. The organism showed a combination of chemotaxonomic and morphological characteristics consistent with its classification in the genus Gordonia and formed a distinct phyletic line in the Gordonia 16S rRNA gene tree. It was closely related to Gordonia terrae DSM 43249(T) (99.9 % 16S rRNA gene sequence similarity) and Gordonia lacunae DSM 45085(T) (99.3 % 16S rRNA gene sequence similarity) but was distinguished from these strains by a moderate level of DNA-DNA relatedness (63.0 and 54.7 %) and discriminatory phenotypic properties. Based on the data obtained, the isolate B204(T) (=CBMAI 1069(T) = DSM 46679(T)) should therefore be classified as the type strain of a novel species of the genus Gordonia, for which the name Gordonia didemni sp. nov. is proposed.
authors.affiliation: Centro Pluridisciplinar de Pesquisas Químicas Biológicas e Agrícolas, UNICAMP, Av. Alexandre Cazelatto 999, Paulínia, SP, CEP 13148-218, Brazil.|Programa de Pós-Graduação em Genética e Biologia Molecular, UNICAMP, CP 6109, Campinas, SP, 13083-970, Brazil.
authors.forename: Cláudia Beatriz Afonso
authors.initials: CB
authors.lastname: de Menezes
authors.affiliation: Centro Pluridisciplinar de Pesquisas Químicas Biológicas e Agrícolas, UNICAMP, Av. Alexandre Cazelatto 999, Paulínia, SP, CEP 13148-218, Brazil.
authors.forename: Rafael Sanches
authors.initials: RS
authors.lastname: Afonso
authors.affiliation: Embrapa Meio Ambiente, Rodovia SP 340 km 127.5, CP 69, Jaguariúna, SP, CEP 13820-000, Brazil.
authors.forename: Wallace Rafael
authors.initials: WR
authors.lastname: de Souza
authors.affiliation: Embrapa Meio Ambiente, Rodovia SP 340 km 127.5, CP 69, Jaguariúna, SP, CEP 13820-000, Brazil.
authors.forename: Márcia
authors.initials: M
authors.lastname: Parma
authors.affiliation: Embrapa Meio Ambiente, Rodovia SP 340 km 127.5, CP 69, Jaguariúna, SP, CEP 13820-000, Brazil.
authors.forename: Itamar Soares
authors.initials: IS
authors.lastname: de Melo
authors.affiliation: Embrapa Meio Ambiente, Rodovia SP 340 km 127.5, CP 69, Jaguariúna, SP, CEP 13820-000, Brazil.|Agrivalle - Biotecnologia Agrícola, Rua Moisés Lopes Silva 50, Pouso Alegre, MG, CEP 37550-000, Brazil.
authors.forename: Tiago Domingues
authors.initials: TD
authors.lastname: Zucchi
authors.affiliation: Centro Pluridisciplinar de Pesquisas Químicas Biológicas e Agrícolas, UNICAMP, Av. Alexandre Cazelatto 999, Paulínia, SP, CEP 13148-218, Brazil. fabianaf@cpqba.unicamp.br.|Programa de Pós-Graduação em Genética e Biologia Molecular, UNICAMP, CP 6109, Campinas, SP, 13083-970, Brazil. fabianaf@cpqba.unicamp.br.
authors.forename: Fabiana
authors.initials: F
authors.lastname: Fantinatti-Garboggini
mesh_terms: D039903:Actinobacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D000818:Animals; D001938:Brazil; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D014561:Urochordata / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S
keywords: Gordonia didemni; Marine ascidium; Polyphasic taxonomy; São Paulo state