title: Postnatal exposure to PFOS, but not PBDE 99, disturb dopaminergic gene transcription in the mouse CNS.
abstract: The CNS of breast feeding infants and toddlers may be exposed to persistent organic pollutants via lactational transfer. Here, 10 days old mice were exposed to single oral doses of either PFOS, PBDE99 or vehicle control and were examined for changes in dopaminergic gene transcription in CNS tissue collected at 24h or 2 months post exposure.qPCR analyses of brain tissue from mice euthanized 24h post exposure revealed that PFOS affected transcription of Dopamine receptor-D5 (DRD5) in cerebral cortex and Tyrosine hydroxylase (TH) in the hippocampus. At 2 months of age, mice neonatally exposed to PFOS displayed decreased transcription of Dopamine receptor-D2 (DRD2) and TH in hippocampus. No significant changes in any of the tested genes were observed in PBDE99 exposed mice. This indicates that PFOS, but not PBDE99, affects the developing cerebral dopaminergic system at gene transcriptional level in cortex and hippocampus, which may account for some of the mechanistic effects behind the aetiology of neuropsychiatric disorders.
authors.affiliation: Department of Environmental Toxicology, Uppsala University, Norbyvägen 18A, S-752 36 Uppsala, Sweden. Electronic address: stefan.hallgren@ebc.uu.se.
authors.forename: Stefan
authors.initials: S
authors.lastname: Hallgren
authors.affiliation: Department of Environmental Toxicology, Uppsala University, Norbyvägen 18A, S-752 36 Uppsala, Sweden.
authors.forename: Henrik
authors.initials: H
authors.lastname: Viberg
mesh_terms: D017738:Alkanesulfonic Acids / Q000633:toxicity*; D000818:Animals; D000831:Animals, Newborn; D002490:Central Nervous System / Q000187:drug effects* / Q000254:growth & development; D002540:Cerebral Cortex / Q000187:drug effects / Q000254:growth & development; D005466:Fluorocarbons / Q000633:toxicity*; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D055768:Halogenated Diphenyl Ethers / Q000633:toxicity*; D006624:Hippocampus / Q000187:drug effects / Q000254:growth & development; D051379:Mice; D017448:Receptors, Dopamine D2 / Q000235:genetics*; D050641:Receptors, Dopamine D5 / Q000235:genetics*; D014446:Tyrosine 3-Monooxygenase / Q000235:genetics
chemical_list: D017738:Alkanesulfonic Acids; C000600618:DRD2 protein, mouse; C493606:Drd5 protein, mouse; D005466:Fluorocarbons; D055768:Halogenated Diphenyl Ethers; D017448:Receptors, Dopamine D2; D050641:Receptors, Dopamine D5; C086401:pentabromodiphenyl ether; C076994:perfluorooctane sulfonic acid; D014446:Tyrosine 3-Monooxygenase
keywords: Brain growth spurt; Brominated flame retardants; Developmental neurotoxicology; Dopaminergic system; Perfluorinated chemical repellants