title: Effects of microfluidization with ionic liquids on the solubilization and structure of β-D-glucan.
abstract: In order to enhance the solubility of yeast β-D-glucan and enlarge the applicable area, we investigated dynamic high pressure microfluidization (DHPM) treatment with ionic liquids as solvent to prepare water-soluble yeast β-D-glucan (WSG). The solubility of β-D-glucan was greatly impacted by the pressure of DHPM, residence time, and ratio of β-D-glucan and EmimAc. The yield of WSG reached 79.25 % (w/w) with the water solubility of 72.50 % (w/w). The DHPM treatment did not destroy the ionic liquid structure therefore the ionic liquids could be recycled. FTIR and (13)C NMR spectrum results showed that WSG was the polysaccharides with (1→3)-β-D-glycosidic bonds, after modification, the hydrogen bonding was destroyed. The chain conformation of WSG in 0.1M NaNO3 aqueous solutions at 25 °C were studied by laser light scattering and viscometry. The Mark-Houwink equation was established to be [η]=2.09×10(-2) MW(0.63) (cm(3) g(-1)). According to the conformational parameters calculated from wormlike cylinder model, WSG existed as semi-stiff chain in aqueous solution.
authors.affiliation: Institute of Agro-Products Processing Science and Technology, Chinese Academy of Agriculture Sciences, Beijing 100193, China.
authors.forename: Hongzhi
authors.initials: H
authors.lastname: Liu
authors.affiliation: Institute of Agro-Products Processing Science and Technology, Chinese Academy of Agriculture Sciences, Beijing 100193, China.
authors.forename: Yanan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Institute of Agro-Products Processing Science and Technology, Chinese Academy of Agriculture Sciences, Beijing 100193, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Gao
authors.affiliation: Institute of Agro-Products Processing Science and Technology, Chinese Academy of Agriculture Sciences, Beijing 100193, China.
authors.forename: Aimin
authors.initials: A
authors.lastname: Shi
authors.affiliation: Institute of Agro-Products Processing Science and Technology, Chinese Academy of Agriculture Sciences, Beijing 100193, China.
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.affiliation: Institute of Agro-Products Processing Science and Technology, Chinese Academy of Agriculture Sciences, Beijing 100193, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Hu
authors.affiliation: Food Science Department, Cornell University, Ithaca 14850, USA.
authors.forename: Novita
authors.initials: N
authors.lastname: Putri
authors.affiliation: Institute of Agro-Products Processing Science and Technology, Chinese Academy of Agriculture Sciences, Beijing 100193, China.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing 100091, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Fan
authors.affiliation: Institute of Agro-Products Processing Science and Technology, Chinese Academy of Agriculture Sciences, Beijing 100193, China. Electronic address: wangqiang06@caas.cn.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wang
mesh_terms: D005936:Glucans / Q000737:chemistry*; D052578:Ionic Liquids / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008968:Molecular Conformation; D015394:Molecular Structure; D008970:Molecular Weight; D012995:Solubility; D012996:Solutions; D017550:Spectroscopy, Fourier Transform Infrared; D014783:Viscosity; D015003:Yeasts / Q000737:chemistry
chemical_list: D005936:Glucans; D052578:Ionic Liquids; D012996:Solutions
keywords: Solubilization; Structure; Yeast β-d-glucan