title: Condensin Relocalization from Centromeres to Chromosome Arms Promotes Top2 Recruitment during Anaphase.
abstract: Condensin is a conserved chromosomal complex necessary to promote mitotic chromosome condensation and sister chromatid resolution during anaphase. Here, we report that yeast condensin binds to replicated centromere regions. We show that centromeric condensin relocalizes to chromosome arms as cells undergo anaphase segregation. We find that condensin relocalization is initiated immediately after the bipolar attachment of sister kinetochores to spindles and requires Polo kinase activity. Moreover, condensin localization during anaphase involves a higher binding rate on DNA and temporally overlaps with condensin's DNA overwinding activity. Finally, we demonstrate that topoisomerase 2 (Top2) is also recruited to chromosome arms during anaphase in a condensin-dependent manner. Our results uncover a functional relation between condensin and Top2 during anaphase to mediate chromosome segregation.
authors.affiliation: Cell Cycle Group, Medical Research Council (MRC), Clinical Sciences Centre, Imperial College London, Hammersmith Hospital, Du Cane Road, London W12 0NN, UK.
authors.forename: Joanne
authors.initials: J
authors.lastname: Leonard
authors.affiliation: Cell Cycle Group, Medical Research Council (MRC), Clinical Sciences Centre, Imperial College London, Hammersmith Hospital, Du Cane Road, London W12 0NN, UK.
authors.forename: Nicholas
authors.initials: N
authors.lastname: Sen
authors.affiliation: Cell Cycle Group, Medical Research Council (MRC), Clinical Sciences Centre, Imperial College London, Hammersmith Hospital, Du Cane Road, London W12 0NN, UK.
authors.forename: Raul
authors.initials: R
authors.lastname: Torres
authors.affiliation: Research Center for Epigenetic Disease, Institute of Molecular and Cellular Biosciences, The University of Tokyo, Tokyo 113-0032, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Sutani
authors.affiliation: Cell Cycle Group, Medical Research Council (MRC), Clinical Sciences Centre, Imperial College London, Hammersmith Hospital, Du Cane Road, London W12 0NN, UK.
authors.forename: Adam
authors.initials: A
authors.lastname: Jarmuz
authors.affiliation: Research Center for Epigenetic Disease, Institute of Molecular and Cellular Biosciences, The University of Tokyo, Tokyo 113-0032, Japan.
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Shirahige
authors.affiliation: Cell Cycle Group, Medical Research Council (MRC), Clinical Sciences Centre, Imperial College London, Hammersmith Hospital, Du Cane Road, London W12 0NN, UK. Electronic address: luis.aragon@csc.mrc.ac.uk.
authors.forename: Luis
authors.initials: L
authors.lastname: Aragón
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000705:Anaphase; D002503:Centromere / Q000378:metabolism*; D020090:Chromosome Segregation; D015825:Chromosomes, Fungal / Q000378:metabolism*; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D004250:DNA Topoisomerases, Type II / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism*; D046912:Multiprotein Complexes / Q000378:metabolism*; D011485:Protein Binding; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism
chemical_list: D004268:DNA-Binding Proteins; D046912:Multiprotein Complexes; D029701:Saccharomyces cerevisiae Proteins; C108312:condensin complexes; D004247:DNA; D000251:Adenosine Triphosphatases; D004250:DNA Topoisomerases, Type II