title: Plasma protein thiols: an early marker of oxidative stress in asthma and chronic obstructive pulmonary disease.
abstract: Chronic obstructive pulmonary disease (COPD) and asthma are both characterized by heterogeneous chronic airway inflammation and obstruction as well as oxidative stress (OS). However, it is unknown whether OS occurs in early disease and how to best assess its presence. Plasma OS markers (TBARS, PSH, taurine, GSH, ergothioneine and paraoxonase 1 activity) and lung function tests were measured in patients with mild stable asthma (n = 24) and mild stable COPD (n = 29) and in age- and sex-matched controls. Forced expiratory volume in 1 s (FEV1 ) was associated with age both in patients and control groups. By contrast, FEV1 was positively correlated with PSH only in COPD (ρ = 0·49, P = 0·007). In multiple logistic regression analysis, lower PSH was the only OS marker independently associated with increased odds of both asthma (OR = 0·32, 95% CI 0·13-0·78, P = 0·01) and COPD (OR = 0·50, 95% CI 0·26-0·95, P = 0·03). These findings suggest that proteins -SH are a sensitive OS marker in early COPD and asthma.
authors.affiliation: Department of Biomedical Sciences, University of Sassari, Sassari, Italy.
authors.forename: Angelo
authors.initials: A
authors.lastname: Zinellu
authors.affiliation: Department of Clinical and Experimental Medicine, University of Sassari, Sassari, Italy.
authors.forename: Alessandro Giuseppe
authors.initials: AG
authors.lastname: Fois
authors.affiliation: Department of Biomedical Sciences, University of Sassari, Sassari, Italy.
authors.forename: Salvatore
authors.initials: S
authors.lastname: Sotgia
authors.affiliation: Department of Clinical and Experimental Medicine, University of Sassari, Sassari, Italy.
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Zinellu
authors.affiliation: Department of Clinical and Experimental Medicine, University of Sassari, Sassari, Italy.
authors.forename: Fabiana
authors.initials: F
authors.lastname: Bifulco
authors.affiliation: Department of Biomedical Sciences, University of Sassari, Sassari, Italy.
authors.forename: Gianfranco
authors.initials: G
authors.lastname: Pintus
authors.affiliation: Department of Clinical Pharmacology, School of Medicine, Flinders University, Adelaide, SA, Australia.
authors.forename: Arduino A
authors.initials: AA
authors.lastname: Mangoni
authors.affiliation: Department of Biomedical Sciences, University of Sassari, Sassari, Italy.
authors.forename: Ciriaco
authors.initials: C
authors.lastname: Carru
authors.affiliation: Department of Clinical and Experimental Medicine, University of Sassari, Sassari, Italy.
authors.forename: Pietro
authors.initials: P
authors.lastname: Pirina
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D043303:Aryldialkylphosphatase / Q000378:metabolism; D001249:Asthma / Q000378:metabolism* / Q000503:physiopathology; D015415:Biomarkers; D004880:Ergothioneine / Q000378:metabolism; D005260:Female; D005541:Forced Expiratory Volume; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D029424:Pulmonary Disease, Chronic Obstructive / Q000378:metabolism* / Q000503:physiopathology; D012129:Respiratory Function Tests; D013438:Sulfhydryl Compounds / Q000378:metabolism*; D013654:Taurine / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014797:Vital Capacity
chemical_list: D015415:Biomarkers; D013438:Sulfhydryl Compounds; D017392:Thiobarbituric Acid Reactive Substances; D013654:Taurine; D004880:Ergothioneine; D043303:Aryldialkylphosphatase; C468406:PON1 protein, human; D005978:Glutathione
keywords: Asthma; COPD; early marker; oxidative stress; proteins -SH