title: Promoter isolation and characterization of GhAO-like1, a Gossypium hirsutum gene similar to multicopper oxidases that is highly expressed in reproductive organs.
abstract: Cotton is one of the most economically important cultivated crops. It is the major source of natural fiber for the textile industry and an important target for genetic modification for both biotic stress and herbicide tolerance. Therefore, the characterization of genes and regulatory regions that might be useful for genetic transformation is indispensable. The isolation and characterization of new regulatory regions is of great importance to drive transgene expression in genetically modified crops. One of the major drawbacks in cotton production is pest damage; therefore, the most promising, cost-effective, and sustainable method for pest control is the development of genetically resistant cotton lines. Considering this scenario, our group isolated and characterized the promoter region of a MCO (multicopper oxidase) from Gossypium hirsutum, named GhAO-like1 (ascorbate oxidase-like1). The quantitative expression, together with the in vivo characterization of the promoter region reveals that GhAO-like1 has a flower- and fruit-specific expression pattern. The GUS activity is mainly observed in stamens, as expected considering that the GhAO-like1 regulatory sequence is enriched in cis elements, which have been characterized as a target of reproductive tissue specific transcription factors. Both histological and quantitative analyses in Arabidopsis thaliana have confirmed flower (mainly in stamens) and fruit expression of GhAO-like1. In the present paper, we isolated and characterized both in silico and in vivo the promoter region of the GhAO-like1 gene. The regulatory region of GhAO-like1 might be useful to confer tissue-specific expression in genetically modified plants.
authors.affiliation: a Department of Genetics, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, Brazil.
authors.forename: Julia
authors.initials: J
authors.lastname: Lambret-Frotté
authors.affiliation: a Department of Genetics, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, Brazil.
authors.forename: Sinara
authors.initials: S
authors.lastname: Artico
authors.affiliation: a Department of Genetics, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, Brazil.
authors.forename: Sarah
authors.initials: S
authors.lastname: Muniz Nardeli
authors.affiliation: b Embrapa Genetic Resources and Biotechnology, Brasilia, DF, Brazil.
authors.forename: Fernando
authors.initials: F
authors.lastname: Fonseca
authors.affiliation: b Embrapa Genetic Resources and Biotechnology, Brasilia, DF, Brazil.
authors.forename: Osmundo
authors.initials: O
authors.lastname: Brilhante Oliveira-Neto
authors.affiliation: b Embrapa Genetic Resources and Biotechnology, Brasilia, DF, Brazil.|c Universidade Católica de Brasília (UCB), Brasilia, DF, Brazil.
authors.forename: Maria Fatima
authors.initials: MF
authors.lastname: Grossi-de-Sá
authors.affiliation: a Department of Genetics, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, Brazil.
authors.forename: Marcio
authors.initials: M
authors.lastname: Alves-Ferreira
mesh_terms: D017360:Arabidopsis / Q000235:genetics; D035264:Flowers / Q000235:genetics; D005638:Fruit / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D003368:Gossypium / Q000235:genetics*; D009928:Organ Specificity; D010088:Oxidoreductases / Q000235:genetics*; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic
chemical_list: D010940:Plant Proteins; D010088:Oxidoreductases
keywords: coton; cotton; développement du pollen; expression hétérologue; genome walking; heterologous expression; marche chromosomique; pollen development; qPCR