title: Thermal manipulation during broiler chicken embryogenesis: Effect on mRNA expressions of Hsp108, Hsp70, Hsp47 and Hsf-3 during subsequent post-hatch thermal challenge.
abstract: Effects of thermal manipulation during broiler chicken embryonic days 12-18 on body temperature (T(b)) and mRNA expressions of Hsp108, Hsp70, Hsp47 and Hsf-3 in muscle, heart and brain tissues during subsequent thermal challenge (TC) were investigated. Fertile chicken eggs were divided randomly into four groups (n=375): eggs in the control group were maintained at 37.8°C and 56% (RH). Eggs in TM1 group were subjected to TM at 39°C for 9h during ED 12-18. Eggs in the TM2 and TM3 groups were subjected to the same protocol of TM1 except for increasing the period of exposure to 12h and 18h, respectively. During TC (43°C for 6h) at days 10 and 28, T(b) of TM chicks was significantly lower compared to controls. Furthermore, significant changes in mRNA expressions of Hsp108, Hsp70 and Hsp47 in muscle, heart and brain tissues were observed.
authors.affiliation: Department of Anatomy, Faculty of Veterinary Medicine and Animal Resources, King Faisal University, Al-Hasa, Saudi Arabia. Electronic address: alzghoull@just.edu.jo.
authors.forename: Mohammad-Borhan
authors.initials: MB
authors.lastname: Al-Zghoul
authors.affiliation: Department of Anatomy, Faculty of Veterinary Medicine and Animal Resources, King Faisal University, Al-Hasa, Saudi Arabia.
authors.forename: Abd Elhafeed S
authors.initials: AE
authors.lastname: Dalab
authors.affiliation: Department of Anatomy, Faculty of Veterinary Medicine and Animal Resources, King Faisal University, Al-Hasa, Saudi Arabia.
authors.forename: Imaad E
authors.initials: IE
authors.lastname: Yahya
authors.affiliation: Department of Anatomy, Faculty of Veterinary Medicine and Animal Resources, King Faisal University, Al-Hasa, Saudi Arabia.
authors.forename: Thnaian A
authors.initials: TA
authors.lastname: Althnaian
authors.affiliation: Department of Anatomy, Faculty of Veterinary Medicine and Animal Resources, King Faisal University, Al-Hasa, Saudi Arabia.
authors.forename: Saeed Y
authors.initials: SY
authors.lastname: Al-Ramadan
authors.affiliation: Department of Anatomy, Faculty of Veterinary Medicine and Animal Resources, King Faisal University, Al-Hasa, Saudi Arabia.
authors.forename: Abdelhadi M
authors.initials: AM
authors.lastname: Ali
authors.affiliation: Department of Physiology and Pharmacology, Faculty of Veterinary Medicine and Animal Resources, King Faisal University, Al-Hasa, Saudi Arabia.
authors.forename: Ibrahim F
authors.initials: IF
authors.lastname: Albokhadaim
authors.affiliation: Department of Physiology and Pharmacology, Faculty of Veterinary Medicine and Animal Resources, King Faisal University, Al-Hasa, Saudi Arabia.
authors.forename: Sabry M
authors.initials: SM
authors.lastname: El-Bahr
authors.affiliation: Department of Physiology and Pharmacology, Faculty of Veterinary Medicine and Animal Resources, King Faisal University, Al-Hasa, Saudi Arabia.
authors.forename: Khalid Ahmed
authors.initials: KA
authors.lastname: Al Busadah
authors.affiliation: Department of Basic Medical Sciences, Purdue University, West Lafayette, IN, United States.
authors.forename: Kevin M
authors.initials: KM
authors.lastname: Hannon
mesh_terms: D000818:Animals; D030161:Avian Proteins / Q000235:genetics* / Q000378:metabolism; D001831:Body Temperature; D001921:Brain / Q000378:metabolism; D002642:Chick Embryo / Q000254:growth & development / Q000378:metabolism / Q000502:physiology; D002645:Chickens / Q000235:genetics* / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D047108:Embryonic Development; D015870:Gene Expression; D050958:HSP47 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000235:genetics* / Q000378:metabolism; D006358:Hot Temperature; D018482:Muscle, Skeletal / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D030161:Avian Proteins; D004268:DNA-Binding Proteins; D050958:HSP47 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D012333:RNA, Messenger; D014157:Transcription Factors
keywords: Heat shock factor-3; Heat shock protein 108; Heat shock protein 47; Heat shock protein 70; Thermal manipulation