title: Protective Efficacy of an Inactive Vaccine Based on the LY02 Isolate against Acute Haemophilus parasuis Infection in Piglets.
abstract: Haemophilus parasuis can cause Glässer's disease characterized by fibrinous polyserositis, polyarthritis, and meningitis. The current prevention of Glässer's disease is mainly based on the inactive vaccines; however, the protective efficacy usually fails in heterogeneous or homologous challenges. Here, the predominant lineage of H. parasuis (LY02 strain) in Fujian province, China, characterized as serovar 5, was used to evaluate the protective immunity against acute H. parasuis infection in piglets after inactivation. Following challenging with H. parasuis, only mild lesions in the pigs immunized with the killed vaccine were observed, whereas the typical symptoms of Glässer's disease presented in the nonimmunized piglets. A strong IgG immune response was induced by the inactive vaccine. CD4(+) and CD8(+) T lymphocyte levels were increased, indicating the potent cellular immune responses were elicited. The significantly high levels of IL-2, IL-4, TGF-β, and IFN-γ in sera from pigs immunized with this killed vaccine suggested that the mixed Th1 and Th2 immune responses were induced, associated with the high protection against H. parasuis infection compared to the nonimmunized animals. This study indicated that the inactivated LY02 strain of H. parasuis could serve as a potential vaccine candidate to prevent the prevalence of H. parasuis in Fujian province, China.
authors.affiliation: College of Life Science, Longyan University, Fujian Provincial Key Laboratory of Preventive Veterinary Medicine and Veterinary Biotechnology, Fujian Engineering Research Center for Swine Disease Control and Prevention, Longyan, Fujian 364012, China.
authors.forename: Xiao-Hua
authors.initials: XH
authors.lastname: Li
authors.affiliation: College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China.
authors.forename: Guo-Zhen
authors.initials: GZ
authors.lastname: Zhao
authors.affiliation: College of Life Science, Longyan University, Fujian Provincial Key Laboratory of Preventive Veterinary Medicine and Veterinary Biotechnology, Fujian Engineering Research Center for Swine Disease Control and Prevention, Longyan, Fujian 364012, China.
authors.forename: Long-Xin
authors.initials: LX
authors.lastname: Qiu
authors.affiliation: College of Life Science, Longyan University, Fujian Provincial Key Laboratory of Preventive Veterinary Medicine and Veterinary Biotechnology, Fujian Engineering Research Center for Swine Disease Control and Prevention, Longyan, Fujian 364012, China.
authors.forename: Ai-Ling
authors.initials: AL
authors.lastname: Dai
authors.affiliation: College of Life Science, Longyan University, Fujian Provincial Key Laboratory of Preventive Veterinary Medicine and Veterinary Biotechnology, Fujian Engineering Research Center for Swine Disease Control and Prevention, Longyan, Fujian 364012, China.
authors.forename: Wang-Wei
authors.initials: WW
authors.lastname: Wu
authors.affiliation: College of Life Science, Longyan University, Fujian Provincial Key Laboratory of Preventive Veterinary Medicine and Veterinary Biotechnology, Fujian Engineering Research Center for Swine Disease Control and Prevention, Longyan, Fujian 364012, China.
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Yang
mesh_terms: D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D016207:Cytokines / Q000276:immunology; D006192:Haemophilus Infections / Q000276:immunology / Q000517:prevention & control* / Q000662:veterinary; D018073:Haemophilus Vaccines / Q000276:immunology / Q000494:pharmacology*; D044137:Haemophilus parasuis / Q000276:immunology* / Q000302:isolation & purification; D013552:Swine; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology; D014613:Vaccines, Attenuated / Q000276:immunology / Q000494:pharmacology
chemical_list: D016207:Cytokines; D018073:Haemophilus Vaccines; D014613:Vaccines, Attenuated