title: Novel functional polysaccharides from Radix Polygoni Multiflori water extracted residue: Preliminary characterization and immunomodulatory activity.
abstract: The alkali-extractable polysaccharides (APMPs) were isolated from the water extracted residues of Radix Polygoni Multiflori, and further purified by DEAE-52 cellulose and Sephadex G-100 column chromatography to obtain a homogeneous polysaccharide (APMP-2) with molecular weights of 7724.8 Da. HPLC chromatography analysis identified that APMP-2 was a heteropolysaccharides and mainly composed of Galactose and Xylose with a molar ratio of 4.31: 1.06. It was shown that both APMP and APMP-2 were of activation effects on splenocytes and peritoneal macrophages, and also significantly restore the proliferation rate, phagocytic index and cytokine (IL-2 and TNF-α) production level of 5-FU-treated splenocytes/peritoneal macrophages in a dosage-dependent manner. The results suggested that polysaccharides presented in Radix Polygoni Multiflori water-extracted residues possessed immunomodulatory activity and could be used as potential immunomodulators, and this finding could be a reference for the utilization of Radix Polygoni Multiflori water extracted residues.
authors.affiliation: School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 400030, PR China; Defense Key Disciplines Lab of Novel Micro-nano Devices and System Technology, Chongqing 400030, PR China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 400030, PR China; Defense Key Disciplines Lab of Novel Micro-nano Devices and System Technology, Chongqing 400030, PR China; International R&D Center of Micro-nano Systems and New Materials Technology, Chongqing 400030, PR China. Electronic address: xuyibbd@sina.com.
authors.forename: Yi
authors.initials: Y
authors.lastname: Xu
authors.affiliation: School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 400030, PR China; Defense Key Disciplines Lab of Novel Micro-nano Devices and System Technology, Chongqing 400030, PR China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Zou
authors.affiliation: School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 400030, PR China; Defense Key Disciplines Lab of Novel Micro-nano Devices and System Technology, Chongqing 400030, PR China.
authors.forename: Xiaodan
authors.initials: X
authors.lastname: Zhang
authors.affiliation: School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 400030, PR China; Defense Key Disciplines Lab of Novel Micro-nano Devices and System Technology, Chongqing 400030, PR China.
authors.forename: Kun
authors.initials: K
authors.lastname: Cao
authors.affiliation: School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 400030, PR China; Defense Key Disciplines Lab of Novel Micro-nano Devices and System Technology, Chongqing 400030, PR China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Fan
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005260:Female; D007155:Immunologic Factors / Q000737:chemistry / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D051379:Mice; D028502:Polygonum / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D013154:Spleen / Q000166:cytology / Q000187:drug effects; D014867:Water / Q000737:chemistry
chemical_list: D007155:Immunologic Factors; D011134:Polysaccharides; D014867:Water
keywords: Alkali-extractable polysaccharides; Immunomodulatory activity; Radix Polygoni Multiflori; Water extracted residues