title: Chloroplast Hsp93 Directly Binds to Transit Peptides at an Early Stage of the Preprotein Import Process.
abstract: Three stromal chaperone ATPases, cpHsc70, Hsp90C, and Hsp93, are present in the chloroplast translocon, but none has been shown to directly bind preproteins in vivo during import, so it remains unclear whether any function as a preprotein-translocating motor and whether they have different functions during the import process. Here, using protein crosslinking followed by ionic detergent solubilization, we show that Hsp93 directly binds to the transit peptides of various preproteins undergoing active import into chloroplasts. Hsp93 also binds to the mature region of a preprotein. A time course study of import, followed by coimmunoprecipitation experiments, confirmed that Hsp93 is present in the same complexes as preproteins at an early stage when preproteins are being processed to the mature size. In contrast, cpHsc70 is present in the same complexes as preproteins at both the early stage and a later stage after the transit peptide has been removed, suggesting that cpHsc70, but not Hsp93, is important in translocating processed mature proteins across the envelope.
authors.affiliation: Institute of Molecular Biology, Academia Sinica, Nankang, Taipei 11529, Taiwan.
authors.forename: Po-Kai
authors.initials: PK
authors.lastname: Huang
authors.affiliation: Institute of Molecular Biology, Academia Sinica, Nankang, Taipei 11529, Taiwan.
authors.forename: Po-Ting
authors.initials: PT
authors.lastname: Chan
authors.affiliation: Institute of Molecular Biology, Academia Sinica, Nankang, Taipei 11529, Taiwan.
authors.forename: Pai-Hsiang
authors.identifier: http://orcid.org/0000-0002-0400-0556
authors.initials: PH
authors.lastname: Su
authors.affiliation: Institute of Molecular Biology, Academia Sinica, Nankang, Taipei 11529, Taiwan.
authors.forename: Lih-Jen
authors.initials: LJ
authors.lastname: Chen
authors.affiliation: Institute of Molecular Biology, Academia Sinica, Nankang, Taipei 11529, Taiwan mbhmli@gate.sinica.edu.tw.
authors.forename: Hsou-min
authors.identifier: http://orcid.org/0000-0002-0211-7339
authors.initials: HM
authors.lastname: Li
mesh_terms: D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D060365:Chloroplast Proteins / Q000235:genetics / Q000378:metabolism*; D002736:Chloroplasts / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D018832:Molecular Chaperones / Q000235:genetics / Q000378:metabolism*; D018532:Pisum sativum / Q000378:metabolism; D010455:Peptides / Q000378:metabolism; D021381:Protein Transport
chemical_list: D029681:Arabidopsis Proteins; D060365:Chloroplast Proteins; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; D010455:Peptides; C574007:AT5G50920 protein, Arabidopsis