title: Programming a nonvolatile memory-like sensor for KRAS gene sensing and signal enhancement.
abstract: A programmable field effect-based electrolyte-insulator-semiconductor (EIS) sensor constructed with a nonvolatile memory-like structure is proposed for KRAS gene DNA hybridization detection. This programmable EIS structure was fabricated with silicon oxide (SiO2)/silicon nitride (Si3N4)/silicon oxide on a p-type silicon wafer, namely electrolyte-oxide-nitride-oxide-Si (EONOS). In this research, voltage stress programming from 4 to 20V was applied to trigger holes confinement in the nitride-trapping layer that, consequently, enhances the DNA attachment onto the sensing surface due to additional electrostatic interaction. Not solely resulting from the higher DNA load, the programming may affect the orientation of the DNA that finally contributes to the change in capacitance. Findings have shown that a higher voltage program is able to increase the total capacitance and results in ~3.5- and ~5.5-times higher sensitivities for a series of concentrations for complementary DNA and wild type versus mutant DNA hybridization detection, respectively. Overall, it has been proven that the voltage program on the nonvolatile memory-like structure of EONOS is a notable candidate for genosensor development, scoping the diagnosis of a single nucleotide polymorphism (SNP)-related disease.
authors.affiliation: Department of Electronic Engineering, Chang-Gung University, Taoyuan, Taiwan.
authors.forename: Yi-Ting
authors.initials: YT
authors.lastname: Lin
authors.affiliation: Biomedical Engineering, Chang-Gung University, Taoyuan, Taiwan.
authors.forename: Agnes
authors.initials: A
authors.lastname: Purwidyantri
authors.affiliation: Department of Medical Biotechnology and Laboratory Science, Chang Gung University, Taoyuan, Taiwan.
authors.forename: Ji-Dung
authors.initials: JD
authors.lastname: Luo
authors.affiliation: Department of Medical Biotechnology and Laboratory Science, Chang Gung University, Taoyuan, Taiwan.
authors.forename: Chiuan-Chian
authors.initials: CC
authors.lastname: Chiou
authors.affiliation: Department of Electronic Engineering, Chang-Gung University, Taoyuan, Taiwan; Institute of Electro-Optical Engineering, Chang Gung University, Taoyuan, Taiwan; Biosensor Group, Biomedical Engineering Research Center, Chang Gung University, Taoyuan, Taiwan. Electronic address: cmyang@mail.cgu.edu.tw.
authors.forename: Chia-Ming
authors.initials: CM
authors.lastname: Yang
authors.affiliation: Department of General Surgery, Chang Gung Memorial Hospital, Linkou, Taiwan.
authors.forename: Chih-Hong
authors.initials: CH
authors.lastname: Lo
authors.affiliation: Department of General Surgery, Chang Gung Memorial Hospital, Linkou, Taiwan.
authors.forename: Tsann-Long
authors.initials: TL
authors.lastname: Hwang
authors.affiliation: Department of Nephrology and Division of Clinical Toxicology, Chang Gung Memorial Hospital, Linkou, Taiwan.
authors.forename: Tzung-Hai
authors.initials: TH
authors.lastname: Yen
authors.affiliation: Department of Electronic Engineering, Chang-Gung University, Taoyuan, Taiwan; Department of General Surgery, Chang Gung Memorial Hospital, Linkou, Taiwan; Department of Materials Engineering, Ming-Chi University of Technology, New Taipei City 243, Taiwan. Electronic address: cslai@mail.cgu.edu.tw.
authors.forename: Chao-Sung
authors.initials: CS
authors.lastname: Lai
mesh_terms: D001483:Base Sequence; D015374:Biosensing Techniques / Q000295:instrumentation*; D004247:DNA / Q000737:chemistry / Q000235:genetics; D025802:Electric Capacitance; D004867:Equipment Design; D006801:Humans; D061925:Immobilized Nucleic Acids / Q000737:chemistry / Q000235:genetics; D009154:Mutation; D009693:Nucleic Acid Hybridization; D020641:Polymorphism, Single Nucleotide; D016283:Proto-Oncogene Proteins p21(ras) / Q000235:genetics*; D012666:Semiconductors; D017655:Silicon Compounds / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry
chemical_list: D061925:Immobilized Nucleic Acids; C117307:KRAS protein, human; D017655:Silicon Compounds; D012822:Silicon Dioxide; D004247:DNA; D016283:Proto-Oncogene Proteins p21(ras); C032734:silicon nitride
keywords: Charge trapping; EIS; EONOS; KRAS gene; Voltage program