title: [The role of Nods like receptors in the patients with allergic rhinitis].
abstract: OBJECTIVE
  To explore role of Nods (nucleotide-binding oligomerization domain Nod Like receptors) kind of pattern recognition receptors (PRR) in patients with allergic rhinitis.
  
  
  METHODS
  The mRNA and protein of Nod1, Nod2 of Nalp3 were analyzed in the turbinate mucosa of patients with allergic rhinitis, nasal septum deviation (NSD) nasal mucosa of patients and nasal polyp mucosa with Real-Time RT-PCR, Western blot and immunohistochemistry respectively, and Nod1 expression changes was explored in PBMC with wad explored Western-blot and then the level of IL-4, IL-6, IL-10, IFN-γ were detected in serum of AR after desensitization treatment.
  
  
  RESULTS
  These Nods like receptors, mainly found in nasal mucosa epithelial cells, glandular epithelium and inflammatory cells (e. g. plasma cells, eosinophils), were expressed in the nasal mucosa tissues. In AR group, Nod1 (mRNA and protein) expression were lower than NSD group (P<0.05), Nalp3 expression were higher than (P<0.05), while, there was no significant difference of Nod2 (mRNA and protein) between groups. After 6 months desensitization therapy, the change of Nod1 in PBMC was negatively correlated with the change of IL-10 in the peripheral blood, r=-0.88, P<0.05; while, change of Nod1 was positively correlated, with the change of IL-6, r=0.57, P>0.05.
  
  
  CONCLUSIONS
  Nod1, Nod2 and Nalp3 expression were seen in the two groups,and the Nod1 expression in allergic rhinitis group was lower than other two groups, while, the Nalp3 was higher than other two groups. It showed Nod1, Nalp3 may be involved in the pathogenesis of allergic rhinitis. Expression of Nod1 in PBMC reduced after sublingual desensitization treatment. Besides, the change of Nod1 was negatively correlated with the change of IL-10 in PBMC. So,it seemed that Nod1 may regulate IL-10 changes and be involved in sublingual desensitization therapy.
authors.forename: Shenhua
authors.initials: S
authors.lastname: Zhang
authors.forename: Yanhui
authors.initials: Y
authors.lastname: Liu
authors.forename: Congxiang
authors.initials: C
authors.lastname: Shen
authors.forename: Guanxue
authors.initials: G
authors.lastname: Li
authors.forename: Keke
authors.initials: K
authors.lastname: Yang
authors.forename: Xin
authors.initials: X
authors.lastname: Shi
authors.forename: Zhong
authors.initials: Z
authors.lastname: Wen
mesh_terms: D002352:Carrier Proteins / Q000378:metabolism*; D006801:Humans; D007371:Interferon-gamma / Q000097:blood; D016753:Interleukin-10 / Q000097:blood; D015847:Interleukin-4 / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; D009297:Nasal Mucosa / Q000378:metabolism; D009298:Nasal Polyps / Q000378:metabolism; D053474:Nod1 Signaling Adaptor Protein / Q000378:metabolism*; D053473:Nod2 Signaling Adaptor Protein / Q000378:metabolism*; D051192:Receptors, Pattern Recognition / Q000378:metabolism*; D065631:Rhinitis, Allergic / Q000378:metabolism*; D014420:Turbinates / Q000378:metabolism
chemical_list: D002352:Carrier Proteins; C573415:IFNG protein, human; C508609:IL10 protein, human; C508598:IL4 protein, human; C508600:IL6 protein, human; D015850:Interleukin-6; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; C453881:NLRP3 protein, human; C119067:NOD1 protein, human; C429018:NOD2 protein, human; D053474:Nod1 Signaling Adaptor Protein; D053473:Nod2 Signaling Adaptor Protein; D051192:Receptors, Pattern Recognition; D016753:Interleukin-10; D015847:Interleukin-4; D007371:Interferon-gamma