title: Relation of Paraoxonase1, Arylesterase and Lipid Profile in Ischemic Stroke Patients.
abstract: BACKGROUND
  Paraoxonase 1 (PON1) is an enzyme associated with High density lipoprotein (HDL) in blood and it is considered to have antioxidant and antiatherogenic properties. PON1 plays an important role in protecting HDL and especially low density lipoprotein (LDL) from oxidative modification by hydrolyzing lipid peroxides which are known to be associated with many vascular diseases including atherosclerosis and ischemic stroke.
  
  
  OBJECTIVE
  The aim of the study was to evaluate and correlate serum paraoxonase (PON1) and arylesterase (ARE) activities as well as lipid profile levels in patients with ischemic stroke.
  
  
  METHODS
  The study population was comprised of 50 ischemic stroke patients and 50 healthy controls. The serum PON1 and ARE activities were measured spectrophotometrically by using paraoxon and phenylacetate as substrate respectively by Eckerson method. Serum lipid was measured using routine biochemical method.
  
  
  RESULTS
  The normality of the distribution of the parameters are assessed by Shapiro-Wilk test. Two sample t-test is applied for hypothesis testing. The serum PONI and arylesterase ARE decreased significantly in ischemic stroke patients (p<0.001). The PON1 was positively correlated with HDL.
  
  
  CONCLUSIONS
  This study strongly suggests that the estimation of HDL-C associated PON1 enzyme gives valuable information for prediction of risk of ischemic stroke due to cerebrovascular thromboembolism. The result shows that PON1 and ARE could be considered as a risk factors for ischemic stroke.
authors.affiliation: Assistant Professor, Department of Biochemistry, SSH & GMCH , Nagpur, India .
authors.forename: Seema Sanjay
authors.initials: SS
authors.lastname: Chawhan
authors.affiliation: Professor and Head, Department of Biochemistry, SRTR, GMCH , Ambajogai, India .
authors.forename: Mukund R
authors.initials: MR
authors.lastname: Mogarekar
authors.affiliation: Associate Professor, Department of Biochemistry, SSH & GMCH , Nagpur, India .
authors.forename: Reena V
authors.initials: RV
authors.lastname: Wagh
authors.affiliation: Assistant Professor, Department of Biochemistry, SSH & GMCH , Nagpur, India, India .
authors.forename: Rajkumar R
authors.initials: RR
authors.lastname: Das
authors.affiliation: Assistant Professor, Department of Biochemistry, SSH & GMCH , Nagpur, India .
authors.forename: Sanjay S
authors.initials: SS
authors.lastname: Pramanik
authors.affiliation: Professor and Head, Department of Biochemistry, SSH & GMCH , Nagpur, India .
authors.forename: Sanjay M
authors.initials: SM
authors.lastname: Sonune
authors.affiliation: Assistant Professor, Department of Pathology, SSH & GMCH , Nagpur, India .
authors.forename: Sanjay M
authors.initials: SM
authors.lastname: Chawhan
keywords: Atherosclerosis; HDL; LDL oxidation; Paraoxon