title: Developmental competence of oocytes grown in vitro: Has it peaked already?
abstract: In vitro growth of immature oocytes provides opportunities to increase gametic resources and to understand the mechanisms underlying oocyte development. Many studies on the in vitro growth of oocytes have been reported thus far; however, only a few cases have been reported, which demonstrated that oocytes can support full-term development after in vitro fertilization. Our research group recently found that culture of mouse neonatal primordial follicles increased the birthrate; however, the establishment of an in vitro system that can completely mimic follicle or oocyte growth in vivo and control oogenesis remains an ongoing challenge.
authors.affiliation: Department of Bioscience, Tokyo University of Agriculture, Tokyo 156-8502, Japan.
authors.forename: Kanako
authors.initials: K
authors.lastname: Morohaku
authors.forename: Yuji
authors.initials: Y
authors.lastname: Hirao
authors.forename: Yayoi
authors.initials: Y
authors.lastname: Obata
mesh_terms: D000818:Animals; D003470:Culture Media / Q000737:chemistry; D003593:Cytoplasm / Q000378:metabolism; D005260:Female; D005307:Fertilization in Vitro; D005854:Germ Cells / Q000166:cytology; D006801:Humans; D051379:Mice; D009865:Oocytes / Q000166:cytology*; D009866:Oogenesis; D009924:Organ Culture Techniques / Q000379:methods*; D006080:Ovarian Follicle / Q000166:cytology*; D034622:RNA Interference; D018456:Reproductive Medicine / Q000379:methods; D059467:Transcriptome
chemical_list: D003470:Culture Media