title: Effector-Mining in the Poplar Rust Fungus Melampsora larici-populina Secretome.
abstract: The poplar leaf rust fungus, Melampsora larici-populina has been established as a tree-microbe interaction model. Understanding the molecular mechanisms controlling infection by pathogens appears essential for durable management of tree plantations. In biotrophic plant-parasites, effectors are known to condition host cell colonization. Thus, investigation of candidate secreted effector proteins (CSEPs) is a major goal in the poplar-poplar rust interaction. Unlike oomycetes, fungal effectors do not share conserved motifs and candidate prediction relies on a set of a priori criteria established from reported bona fide effectors. Secretome prediction, genome-wide analysis of gene families and transcriptomics of M. larici-populina have led to catalogs of more than a thousand secreted proteins. Automatized effector-mining pipelines hold great promise for rapid and systematic identification and prioritization of CSEPs for functional characterization. In this review, we report on and discuss the current status of the poplar rust fungus secretome and prediction of candidate effectors from this species.
authors.affiliation: INRA, UMR 1136 Interactions Arbres/Microorganismes INRA/Université de Lorraine, Centre INRA Nancy Lorraine , Champenoux, France ; Université de Lorraine, UMR 1136 Interactions Arbres/Microorganismes Université de Lorraine/INRA, Faculté des Sciences et Technologies , Vandoeuvre-lès-Nancy, France.
authors.forename: Cécile
authors.initials: C
authors.lastname: Lorrain
authors.affiliation: INRA, UMR 1136 Interactions Arbres/Microorganismes INRA/Université de Lorraine, Centre INRA Nancy Lorraine , Champenoux, France ; Université de Lorraine, UMR 1136 Interactions Arbres/Microorganismes Université de Lorraine/INRA, Faculté des Sciences et Technologies , Vandoeuvre-lès-Nancy, France.
authors.forename: Arnaud
authors.initials: A
authors.lastname: Hecker
authors.affiliation: INRA, UMR 1136 Interactions Arbres/Microorganismes INRA/Université de Lorraine, Centre INRA Nancy Lorraine , Champenoux, France ; Université de Lorraine, UMR 1136 Interactions Arbres/Microorganismes Université de Lorraine/INRA, Faculté des Sciences et Technologies , Vandoeuvre-lès-Nancy, France.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Duplessis
keywords: effector protein; expert annotation; multigene families analysis; poplar rust; prediction pipeline