title: Toxicity and oxidative stress induced by T-2 toxin and HT-2 toxin in broilers and broiler hepatocytes.
abstract: T-2 and HT-2 toxins belong to mycotoxins which are found in human foods and animal chow. We investigated the toxicity and oxidative stress induced by T-2/HT-2 in broilers and chicken hepatocytes. Maize cultures of Fusarium poae was fed to broilers for 42 d, and the physiological index, biochemical index and expression of mRNAs related to oxidative stress were analyzed. Chicken hepatocytes were treated with different levels of T-2/HT-2, and the following parameters were detected: cell viability, GSH and MDA concentration, LDH leakage, activities of ALT/AST, ROS, GSH-PX, SOD and CAT, and expression of mRNA related to oxidative stress. In vivo, high levels of mycotoxins (4 mg/kg T-2 and 0.667 mg/kg HT-2) in feed caused significant reductions in body weight, weight gain, and serum total protein, and significant increases in feed conversion ratio, ALP, ALT/AST activities, and expression of mRNA related to oxidative stress. In vitro, cells treated with T-2/HT-2 showed reductions of GSH concentration and significant increases in LDH leakage, ALT/AST ROS, GSH-PX, SOD and CAT activities, MDA concentration, and expression of mRNA related to oxidative stress. Consequently, F. poae culture material and T-2/HT-2 induced toxicity and oxidative stress in vivo and in vitro, respectively.
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, No. 1 Wei-Gang, Xuanwu District, Nanjing 210095, PR China.
authors.forename: Lingchen
authors.initials: L
authors.lastname: Yang
authors.affiliation: Department of Animal Science, Yuxi Agricultural Vocation Technical College, No. 41 Xiangjiazhuang, Hongta District, Yuxi 653100, PR China.
authors.forename: Zezhong
authors.initials: Z
authors.lastname: Yu
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, No. 1 Wei-Gang, Xuanwu District, Nanjing 210095, PR China. Electronic address: jfhou@njau.edu.cn.
authors.forename: Jiafa
authors.initials: J
authors.lastname: Hou
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, No. 1 Wei-Gang, Xuanwu District, Nanjing 210095, PR China.
authors.forename: Yifeng
authors.initials: Y
authors.lastname: Deng
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, No. 1 Wei-Gang, Xuanwu District, Nanjing 210095, PR China.
authors.forename: Zhenlei
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, No. 1 Wei-Gang, Xuanwu District, Nanjing 210095, PR China.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, No. 1 Wei-Gang, Xuanwu District, Nanjing 210095, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Cui
mesh_terms: D000410:Alanine Transaminase; D000818:Animals; D001219:Aspartate Aminotransferases; D002470:Cell Survival / Q000187:drug effects; D002645:Chickens; D004435:Eating / Q000187:drug effects; D005260:Female; D005506:Food Contamination; D005786:Gene Expression Regulation / Q000187:drug effects; D022781:Hepatocytes / Q000187:drug effects*; D007770:L-Lactate Dehydrogenase; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D015394:Molecular Structure; D018384:Oxidative Stress / Q000187:drug effects*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013605:T-2 Toxin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000633:toxicity*; D015430:Weight Gain / Q000187:drug effects; D003313:Zea mays / Q000737:chemistry
chemical_list: D012333:RNA, Messenger; D007770:L-Lactate Dehydrogenase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D013605:T-2 Toxin; C012351:HT-2 toxin
keywords: Broilers; Hepatocytes; Oxidative stress; T-2 and HT-2 toxin; Toxicity