title: Overexpression of interleukins IL-17 and IL-8 with poor prognosis in colorectal cancer induces metastasis.
abstract: Current evidences indicated that a group of soluble mediators called chemokines is involved in tumor growth and metastasis. The association of IL-8 with tumor cell migration was previously found, and its expression was related to angiogenesis, tumor progression, and metastasis in many kinds of carcinomas in human and animal models. Furthermore, it has been showed that IL-17 plays its role as either a proteome of tumor progression or antitumor indifferent cancer models. To investigate the messenger RNA (mRNA) expressions of IL-8 and IL-17 in patients with colorectal cancer (CRC) and non-tumor tissue, quantitative real-time PCR was used in the study. Our results showed that expression of IL-8 mRNA was significantly increased in tumor tissues (mean ± SD 3.84 ± 0.08) compared with adjacent normal mucosa (mean ± SD 1.17 ± 0.75, P = 0.001). Furthermore, a higher expression of IL-17 mRNA was found in tumor tissues (mean ± SD 2.73 ± 0.69) when compared with normal tissues (mean ± SD 1.06 ± 0.07, P = 0.001). Our findings indicated that advanced tumor-node-metastasis (TNM) stage (P = 0.024) and histological grade (poorly differentiated, P = 0.013) and distant metastasis (P = 0.001) were correlated with expression of IL-8. Moreover, high expression of IL-17 showed significant association with early stage CRC (TNM) (P = 0.038). In conclusion, the expression of IL-8 and IL-17 mRNAs was significantly increased in tumor tissues compared with adjacent normal tissues. We found that advanced TNM stage and histological grade and distant metastasis were correlated with expression of IL-8, while high expression of IL-17 showed significant association with early stage CRC (TNM) stage and overexpression of IL-8 may be associated with progression of CRC.
authors.affiliation: Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Lorestan University of Medical Sciences, Khorramabad, Iran.
authors.forename: Mohsen
authors.initials: M
authors.lastname: Mohammadi
authors.affiliation: Department of Biology, Jundishapur University of Medical Sciences, Ahvaz, Iran.
authors.forename: Maria
authors.initials: M
authors.lastname: Kaghazian
authors.affiliation: Department of Pathology, Be'sat Hospital, AJA University of Medical Sciences, Tehran, Iran.
authors.forename: Omid
authors.initials: O
authors.lastname: Rahmani
authors.affiliation: Department of Emergency Medicine, Alborz University of Medical Sciences, Karaj, Iran.
authors.forename: Koorosh
authors.initials: K
authors.lastname: Ahmadi
authors.affiliation: Department of Pathology, Loghman Hakim Hospital, Sarakhs, Iran.
authors.forename: Elham
authors.initials: E
authors.lastname: Hatami
authors.affiliation: Department of Pathology, Be'sat Hospital, AJA University of Medical Sciences, Tehran, Iran.
authors.forename: Katayoun
authors.initials: K
authors.lastname: Ziari
authors.affiliation: Department of Surgery, AJA University of Medical Sciences, Tehran, Iran. dr.amirtalebreza@gmail.com.
authors.forename: Amir
authors.initials: A
authors.lastname: Talebreza
keywords: Colorectal cancer; Expression; IL-17; IL-8; PCR