title: Prophage-mediated modulation of interaction of Streptococcus thermophilus J34 with human intestinal epithelial cells and its competition against human pathogens.
abstract: The human intestinal microbiota plays an important role in human health. While adhesion to gastrointestinal mucosa is a prerequisite for colonisation, inhibition of adhesion is a property which may prevent or reduce infections by food borne pathogens. Streptococcus thermophilus and Lactobacillus delbrueckii subsp. bulgaricus represent the two lactic bacteria constituting the yoghurt culture. These starter cultures have been claimed to be probiotic. In our study we compared two S. thermophilus strains (i.e. lysogenic strain J34 and corresponding non-lysogenic [prophage-cured] strain J34-6), with respect to (1) their in vitro adhesion properties to HT29 cells and (2) their cell surface hydrophobicities. Effects of the two strains on inhibition of adhesion of the pathogens Listeria monocytogenes Scott A, Staphylococcus aureus 6732 and Salmonella enteritidis S489 were studied in vitro with HT29 cell cultures. Lysogenic strain J34 was shown to be considerably more effective than the non-lysogenic derivative strain J34-6.
authors.affiliation: 1 Department of Microbiology and Biotechnology, Max Rubner Institut (Federal Research Institute of Nutrition and Health), Hermann-Weigmann-Straße 1, 24103 Kiel, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Guigas
authors.affiliation: 2 Chair Organismic Interactions, Interfaculty Institute of Microbiology and Infection Medicine, Tübingen University, Geschwister-Scholl-Platz, 72074 Tübingen, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Faulhaber
authors.affiliation: 3 BAV-Institut für Hygiene und Qualitätssicherung, Hanns-Martin-Schleyer-Str. 25, 77656 Offenburg, Germany.
authors.forename: D
authors.initials: D
authors.lastname: Duerbeck
authors.affiliation: 1 Department of Microbiology and Biotechnology, Max Rubner Institut (Federal Research Institute of Nutrition and Health), Hermann-Weigmann-Straße 1, 24103 Kiel, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Neve
authors.affiliation: 1 Department of Microbiology and Biotechnology, Max Rubner Institut (Federal Research Institute of Nutrition and Health), Hermann-Weigmann-Straße 1, 24103 Kiel, Germany.
authors.forename: K J
authors.initials: KJ
authors.lastname: Heller
mesh_terms: D001422:Bacterial Adhesion; D004847:Epithelial Cells / Q000382:microbiology*; D019073:HT29 Cells; D006801:Humans; D007422:Intestines / Q000166:cytology / Q000382:microbiology*; D008089:Listeria monocytogenes / Q000502:physiology*; D008242:Lysogeny; D039002:Prophages / Q000502:physiology*; D013211:Staphylococcus aureus / Q000502:physiology*; D048249:Streptococcus thermophilus / Q000502:physiology* / Q000821:virology*; D015014:Yogurt / Q000382:microbiology
keywords: Streptococcus thermophilus; human pathogens; in vitro adhesion; lactic acid bacteria