title: Assessment of glutathione peroxidase-1 polymorphisms, oxidative stress and DNA damage in sensitivity-related illnesses.
abstract: OBJECTIVE
  Oxidative stress increase is a key event for development of sensitivity-related illnesses (SRIs). The aim of this work was to evaluate the influence of a genetic variant in the antioxidant enzyme glutathione peroxidase (GPx1) on oxidative stress development in SRIs.
  
  
  METHODS
  GPx1 rs1800668 genotype, as well as glutathione, ubiquinone, and DNA damage were assessed in 34 SRI patients and 36 healthy subjects.
  
  
  RESULTS
  Total glutathione, reduced/oxidized glutathione, and ubiquinone were significantly decreased in cases compared with controls, while DNA fragmentation was significantly increased in patients. However, these differences were not associated to GPx1 genetic background.
  
  
  CONCLUSIONS
  GPx1 rs1800668 polymorphism does not play a major role in SRI-related oxidative stress development.
authors.affiliation: Department of Biomedical and Dental Sciences and Morpho-functional Imaging, Polyclinic University of Messina, Via C. Valeria 1, 98125 Messina, Italy.
authors.forename: Agnese
authors.initials: A
authors.lastname: Gugliandolo
authors.affiliation: Department of Biomedical and Dental Sciences and Morpho-functional Imaging, Polyclinic University of Messina, Via C. Valeria 1, 98125 Messina, Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: Gangemi
authors.affiliation: Department of Biomedical and Dental Sciences and Morpho-functional Imaging, Polyclinic University of Messina, Via C. Valeria 1, 98125 Messina, Italy.
authors.forename: Carlo
authors.initials: C
authors.lastname: Calabrò
authors.affiliation: Department of Biomedical and Dental Sciences and Morpho-functional Imaging, Polyclinic University of Messina, Via C. Valeria 1, 98125 Messina, Italy.
authors.forename: Mercurio
authors.initials: M
authors.lastname: Vecchio
authors.affiliation: Department of Biomedical and Dental Sciences and Morpho-functional Imaging, Polyclinic University of Messina, Via C. Valeria 1, 98125 Messina, Italy.
authors.forename: Debora
authors.initials: D
authors.lastname: Di Mauro
authors.affiliation: Department of Drug Sciences, University of Catania, Viale Andrea Doria, 95100 Catania, Italy.
authors.forename: Marcella
authors.initials: M
authors.lastname: Renis
authors.affiliation: Department of Biomedical and Dental Sciences and Morpho-functional Imaging, Polyclinic University of Messina, Via C. Valeria 1, 98125 Messina, Italy.
authors.forename: Riccardo
authors.initials: R
authors.lastname: Ientile
authors.affiliation: Department of Biomedical and Dental Sciences and Morpho-functional Imaging, Polyclinic University of Messina, Via C. Valeria 1, 98125 Messina, Italy.
authors.forename: Monica
authors.initials: M
authors.lastname: Currò
authors.affiliation: Department of Biomedical and Dental Sciences and Morpho-functional Imaging, Polyclinic University of Messina, Via C. Valeria 1, 98125 Messina, Italy. Electronic address: dcaccamo@unime.it.
authors.forename: Daniela
authors.initials: D
authors.lastname: Caccamo
mesh_terms: D000328:Adult; D004249:DNA Damage; D005260:Female; D005838:Genotype; D005979:Glutathione Peroxidase / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008297:Male; D018777:Multiple Chemical Sensitivity / Q000235:genetics* / Q000378:metabolism; D018384:Oxidative Stress; D011110:Polymorphism, Genetic; D055815:Young Adult; D000094765:Glutathione Peroxidase GPX1
chemical_list: D005979:Glutathione Peroxidase; D000094765:Glutathione Peroxidase GPX1; C000722067:GPX1 protein, human
keywords: Antioxidant enzyme polymorphisms; Comet assay; Erythrocyte glutathione; Idiopathic environmental intolerances; Impaired detoxification systems; Ubiquinone