title: Quantification of a Selective Expansion of T Cell Receptor Vβ by Superantigen Using Real-Time PCR.
abstract: Selective expansion of T cells bearing specific T cell receptor Vβ segments is a hallmark of superantigens. Analyzing Vβ specificity of superantigens is important for characterizing newly discovered superantigens and understanding differential T cell responses to each toxin. Here, we describe a real-time PCR method using SYBR green I and primers specific to Cβ and Vβ genes for an absolute quantification. The established method was applied to quantify a selective expansion of T cell receptor Vβ expansion by superantigens and generated accurate, reproducible, and comparable results.
authors.affiliation: Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, MS, 39762-6100, USA.
authors.forename: Joo Youn
authors.initials: JY
authors.lastname: Park
authors.affiliation: Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, MS, 39762-6100, USA. seo@cvm.msstate.edu.
authors.forename: Keun Seok
authors.initials: KS
authors.lastname: Seo
mesh_terms: D018929:Cell Culture Techniques; D006801:Humans; D008213:Lymphocyte Activation / Q000235:genetics / Q000276:immunology; D060888:Real-Time Polymerase Chain Reaction; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics* / Q000378:metabolism; D018089:Superantigens / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism*
chemical_list: D016693:Receptors, Antigen, T-Cell, alpha-beta; D018089:Superantigens
keywords: Quantitative real-time PCR; Superantigen; T cell receptor Vβ