title: Stable Expression of Basic Fibroblast Growth Factor in Chloroplasts of Tobacco.
abstract: Basic fibroblast growth factor (bFGF) is a multifunctional factor in acceleration of cell proliferation, differentiation and transference, and therefore widely used in clinical applications. In this study, expression vector pWX-Nt03 harboring a codon-optimized bFGF gene was constructed and introduced into the tobacco chloroplasts by particle bombardment. After four rounds of selection, bFGF was proved to integrate into the chloroplast genome of regenerated plants and two of four transgenic plants were confirmed to be homoplastomic by PCR and Southern hybridization. ELISA assay indicated that bFGF represented approximately 0.1% of total soluble protein in the leaves of transplastomic tobacco plants. This is the first report of bFGF expression via chloroplast transformation in model plant, providing an additional option for the production of chloroplast-produced therapeutic proteins.
authors.affiliation: Jilin Provincial Key Laboratory of Agricultural Biotechnology, Agro-Biotechnology Research Institute, Jilin Academy of Agricultural Sciences, No. 1363, Shengtai st., Changchun 130033, China. wangypbio@163.com.
authors.forename: Yun-Peng
authors.initials: YP
authors.lastname: Wang
authors.affiliation: Jilin Provincial Key Laboratory of Agricultural Biotechnology, Agro-Biotechnology Research Institute, Jilin Academy of Agricultural Sciences, No. 1363, Shengtai st., Changchun 130033, China. weizy@cjaas.com.
authors.forename: Zheng-Yi
authors.initials: ZY
authors.lastname: Wei
authors.affiliation: Jilin Provincial Key Laboratory of Agricultural Biotechnology, Agro-Biotechnology Research Institute, Jilin Academy of Agricultural Sciences, No. 1363, Shengtai st., Changchun 130033, China. zhongxf@cjaas.com.
authors.forename: Xiao-Fang
authors.initials: XF
authors.lastname: Zhong
authors.affiliation: Jilin Provincial Key Laboratory of Agricultural Biotechnology, Agro-Biotechnology Research Institute, Jilin Academy of Agricultural Sciences, No. 1363, Shengtai st., Changchun 130033, China. lincj@cjaas.com.
authors.forename: Chun-Jing
authors.initials: CJ
authors.lastname: Lin
authors.affiliation: Institute of Agricultural Quality Standard and Testing Technology, Jilin Academy of Agricultural Sciences, No. 1363, Shengtai st., Changchun 130033, China. yhcai64@163.com.
authors.forename: Yu-Hong
authors.initials: YH
authors.lastname: Cai
authors.affiliation: Faculty of Agronomy, Jilin Agricultural University, No. 2888, Xincheng st., Changchun 130118, China. majian19790106@163.com.
authors.forename: Jian
authors.initials: J
authors.lastname: Ma
authors.affiliation: Jilin Provincial Key Laboratory of Agricultural Biotechnology, Agro-Biotechnology Research Institute, Jilin Academy of Agricultural Sciences, No. 1363, Shengtai st., Changchun 130033, China. yuying0609@126.com.|College of Biological Sciences, China Agricultural University, No. 2 West Yuanmingyuan Road, Beijing 100094, China. yuying0609@126.com.
authors.forename: Yu-Ying
authors.initials: YY
authors.lastname: Zhang
authors.affiliation: Jilin Provincial Key Laboratory of Agricultural Biotechnology, Agro-Biotechnology Research Institute, Jilin Academy of Agricultural Sciences, No. 1363, Shengtai st., Changchun 130033, China. liuyz_g@126.com.
authors.forename: Yan-Zhi
authors.initials: YZ
authors.lastname: Liu
authors.affiliation: Jilin Provincial Key Laboratory of Agricultural Biotechnology, Agro-Biotechnology Research Institute, Jilin Academy of Agricultural Sciences, No. 1363, Shengtai st., Changchun 130033, China. xingsc@cjaas.com.
authors.forename: Shao-Chen
authors.initials: SC
authors.lastname: Xing
mesh_terms: D002736:Chloroplasts / Q000235:genetics* / Q000378:metabolism; D016222:Fibroblast Growth Factor 2 / Q000235:genetics* / Q000378:metabolism; D054628:Genome, Chloroplast; D042822:Genomic Instability; D006801:Humans; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000378:metabolism; D011995:Recombination, Genetic; D014026:Nicotiana / Q000235:genetics* / Q000378:metabolism
chemical_list: D016222:Fibroblast Growth Factor 2
keywords: basic fibroblast growth factor; chloroplast; genetic transformation; green fluorescent protein; tobacco