title: Transferrin Decorated Thermoresponsive Nanogels as Magnetic Trap Devices for Circulating Tumor Cells.
abstract: A rational design of magnetic capturing nanodevices, based on a specific interaction with circulating tumor cells (CTCs), can advance the capturing efficiency and initiate the development of modern smart nanoformulations for rapid isolation and detection of these CTCs from the bloodstream. Therefore, the development and evaluation of magnetic nanogels (MNGs) based on magnetic nanoparticles and linear thermoresponsive polyglycerol for the capturing of CTCs with overexpressed transferrin (Tf(+) ) receptors has been presented in this study. The MNGs are synthesized using a strain-promoted "click" approach which has allowed the in situ surface decoration with Tf-polyethylene glycol (PEG) ligands of three different PEG chain lengths as targeting ligands. An optimal value of around 30% of cells captures is achieved with a linker of eight ethylene glycol units. This study shows the potential of MNGs for the capture of CTCs and the necessity of precise control over the linkage of the targeting moiety to the capturing device.
authors.affiliation: Institute of Chemistry and Biochemistry, Freie Universität Berlin, Takustr. 3, 14195, Berlin, Germany.
authors.forename: Mazdak
authors.initials: M
authors.lastname: Asadian-Birjand
authors.affiliation: LAMAP Laboratorio de Materiales Poliméricos, IMBIV-CONICET, Departamento de Química, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Haya de la Torre y Medina Allende, X5000HUA, Córdoba, Argentina.
authors.forename: Catalina
authors.initials: C
authors.lastname: Biglione
authors.affiliation: Institute of Chemistry and Biochemistry, Freie Universität Berlin, Takustr. 3, 14195, Berlin, Germany.
authors.forename: Julian
authors.initials: J
authors.lastname: Bergueiro
authors.affiliation: LAMAP Laboratorio de Materiales Poliméricos, IMBIV-CONICET, Departamento de Química, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Haya de la Torre y Medina Allende, X5000HUA, Córdoba, Argentina.
authors.forename: Ariel
authors.initials: A
authors.lastname: Cappelletti
authors.affiliation: MAEER's Maharashtra Institute of Pharmacy, Kothrud, Pune, 411038, Maharashtra, India.
authors.forename: Chinmay
authors.initials: C
authors.lastname: Rahane
authors.affiliation: MAEER's Maharashtra Institute of Pharmacy, Kothrud, Pune, 411038, Maharashtra, India.
authors.forename: Govind
authors.initials: G
authors.lastname: Chate
authors.affiliation: MAEER's Maharashtra Institute of Pharmacy, Kothrud, Pune, 411038, Maharashtra, India.
authors.forename: Jayant
authors.initials: J
authors.lastname: Khandare
authors.affiliation: Helmholtz-Zentrum Berlin für Materialien und Energie GmbH, D-14109, Berlin, Germany.
authors.forename: Bastian
authors.initials: B
authors.lastname: Klemke
authors.affiliation: LAMAP Laboratorio de Materiales Poliméricos, IMBIV-CONICET, Departamento de Química, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Haya de la Torre y Medina Allende, X5000HUA, Córdoba, Argentina.
authors.forename: Miriam C
authors.initials: MC
authors.lastname: Strumia
authors.affiliation: Institute of Chemistry and Biochemistry, Freie Universität Berlin, Takustr. 3, 14195, Berlin, Germany.
authors.forename: Marcelo
authors.initials: M
authors.lastname: Calderón
mesh_terms: D002469:Cell Separation / Q000379:methods*; D057930:Click Chemistry; D005782:Gels; D015870:Gene Expression; D005990:Glycerol / Q000737:chemistry*; D006801:Humans; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D009360:Neoplastic Cells, Circulating / Q000737:chemistry* / Q000378:metabolism; D011092:Polyethylene Glycols / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D011990:Receptors, Transferrin / Q000737:chemistry* / Q000235:genetics; D014168:Transferrin / Q000737:chemistry*
chemical_list: D005782:Gels; D058185:Magnetite Nanoparticles; D011108:Polymers; D011990:Receptors, Transferrin; D014168:Transferrin; C043941:polyglycerol; D011092:Polyethylene Glycols; D005990:Glycerol
keywords: cell sorting; circulating tumor cells; thermoresponsive nanogels; transferrin