title: Identification of a gene in Riemerella anatipestifer CH-1 (B739-2187) that contributes to resistance to polymyxin B and evaluation of its mutant as a live attenuated vaccine.
abstract: Riemerella anatipestifer (R. anatipestifer) causes severe perihepatitis, pericarditis, airsacculitis and meningitis in the duck, leading to great economic losses worldwide. Given the increased prevalence of drug-resistance strains, vaccination is the best strategy to prevent R. anatipestifer infection in ducklings. In this study, we identified a gene in R. anatipestifer (B739-2187) that can restore the resistance of the Salmonella phoP mutant to polymyxin B using genetic complementation. Furthermore, the deletion of B739-2187 in R. anatipestifer resulted in a mutant exhibiting increased sensitivity to polymyxin B. The R. anatipestifer B739-2187 mutant did not exhibit phenotypic defects, as indicated by its growth curve, lipopolysaccharide and outer membrane protein profiles, and attachment and invasion of duck embryo fibroblast cells. The duck animal experiments demonstrated that the deletion of B739-2187 significantly decreased the virulence of R. anatipestifer, and the B739-2187 mutant provided 100% protection against challenge with wild-type R. anatipestifer, exhibiting the characteristics of an ideal live vaccine.
authors.affiliation: Institute of Preventive Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan 611130, China; Avian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan 611130, China. Electronic address: xxinzhao@sicau.edu.cn.
authors.forename: Xinxin
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Department of Bioengineering, College of Veterinary Medicine, Sichuan Agricultural University, No.211 Huimin Road, Wenjiang District, Chengdu, Sichuan 611130, China. Electronic address: qing.liu.2@sicau.edu.cn.
authors.forename: Qing
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Institute of Preventive Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan 611130, China. Electronic address: zhangjie199026@163.com.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Institute of Preventive Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan 611130, China. Electronic address: hyacinth216@outlook.com.
authors.forename: Yali
authors.initials: Y
authors.lastname: Luo
authors.affiliation: Institute of Preventive Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan 611130, China. Electronic address: luoyingdy@163.com.
authors.forename: Ying
authors.initials: Y
authors.lastname: Luo
authors.affiliation: Institute of Preventive Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan 611130, China. Electronic address: p19890528@126.com.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Institute of Preventive Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan 611130, China. Electronic address: lipei98989@126.com.
authors.forename: Pei
authors.initials: P
authors.lastname: Li
authors.affiliation: Institute of Preventive Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan 611130, China. Electronic address: kongqiki@163.com.
authors.forename: Qingke
authors.initials: Q
authors.lastname: Kong
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D024881:Drug Resistance, Bacterial; D004372:Ducks; D005260:Female; D045826:Flavobacteriaceae Infections / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary*; D017353:Gene Deletion; D008297:Male; D009154:Mutation; D011112:Polymyxin B / Q000494:pharmacology*; D011201:Poultry Diseases / Q000276:immunology* / Q000382:microbiology / Q000517:prevention & control; D060494:Riemerella / Q000187:drug effects / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity; D014613:Vaccines, Attenuated / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D014774:Virulence
chemical_list: D001426:Bacterial Proteins; D014613:Vaccines, Attenuated; D011112:Polymyxin B
keywords: Attenuated live vaccine; B739-2187 gene; Polymyxin B; Riemerella anatipestifer; Virulence