title: Genome-Wide Analysis of Seed Acid Detergent Lignin (ADL) and Hull Content in Rapeseed (Brassica napus L.).
abstract: A stable yellow-seeded variety is the breeding goal for obtaining the ideal rapeseed (Brassica napus L.) plant, and the amount of acid detergent lignin (ADL) in the seeds and the hull content (HC) are often used as yellow-seeded rapeseed screening indices. In this study, a genome-wide association analysis of 520 accessions was performed using the Q + K model with a total of 31,839 single-nucleotide polymorphism (SNP) sites. As a result, three significant associations on the B. napus chromosomes A05, A09, and C05 were detected for seed ADL content. The peak SNPs were within 9.27, 14.22, and 20.86 kb of the key genes BnaA.PAL4, BnaA.CAD2/BnaA.CAD3, and BnaC.CCR1, respectively. Further analyses were performed on the major locus of A05, which was also detected in the seed HC examination. A comparison of our genome-wide association study (GWAS) results and previous linkage mappings revealed a common chromosomal region on A09, which indicates that GWAS can be used as a powerful complementary strategy for dissecting complex traits in B. napus. Genomic selection (GS) utilizing the significant SNP markers based on the GWAS results exhibited increased predictive ability, indicating that the predictive ability of a given model can be substantially improved by using GWAS and GS.
authors.affiliation: College of Agronomy and Biotechnology, Southwest University, Beibei, Chongqing, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Wang
authors.affiliation: College of Agronomy and Biotechnology, Southwest University, Beibei, Chongqing, China.
authors.forename: Hongju
authors.initials: H
authors.lastname: Jian
authors.affiliation: College of Agronomy and Biotechnology, Southwest University, Beibei, Chongqing, China.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Wei
authors.affiliation: College of Agronomy and Biotechnology, Southwest University, Beibei, Chongqing, China.
authors.forename: Cunmin
authors.initials: C
authors.lastname: Qu
authors.affiliation: College of Agronomy and Biotechnology, Southwest University, Beibei, Chongqing, China.
authors.forename: Xinfu
authors.initials: X
authors.lastname: Xu
authors.affiliation: College of Agronomy and Biotechnology, Southwest University, Beibei, Chongqing, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Lu
authors.affiliation: College of Agronomy and Biotechnology, Southwest University, Beibei, Chongqing, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Qian
authors.affiliation: College of Agronomy and Biotechnology, Southwest University, Beibei, Chongqing, China.
authors.forename: Jiana
authors.initials: J
authors.lastname: Li
authors.affiliation: Institute of Resource Biology and Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: Maoteng
authors.initials: M
authors.lastname: Li
authors.affiliation: College of Agronomy and Biotechnology, Southwest University, Beibei, Chongqing, China.
authors.forename: Liezhao
authors.initials: L
authors.lastname: Liu
mesh_terms: D029741:Brassica rapa / Q000235:genetics*; D002874:Chromosome Mapping; D017343:Genes, Plant; D055106:Genome-Wide Association Study; D008031:Lignin / Q000235:genetics*; D015810:Linkage Disequilibrium; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D040641:Quantitative Trait Loci; D019655:Quantitative Trait, Heritable; D012639:Seeds / Q000235:genetics*; D012641:Selection, Genetic
chemical_list: D008031:Lignin