title: Protective Role of Proton-Sensing TDAG8 in Lipopolysaccharide-Induced Acute Lung Injury.
abstract: Acute lung injury is characterized by the infiltration of neutrophils into lungs and the subsequent impairment of lung function. Here we explored the role of TDAG8 in lung injury induced by lipopolysaccharide (LPS) administrated intratracheally. In this model, cytokines and chemokines released from resident macrophages are shown to cause neutrophilic inflammation in the lungs. We found that LPS treatment increased TDAG8 expression in the lungs and confirmed its expression in resident macrophages in bronchoalveolar lavage (BAL) fluids. LPS administration remarkably increased neutrophil accumulation without appreciable change in the resident macrophages, which was associated with increased penetration of blood proteins into BAL fluids, interstitial accumulation of inflammatory cells, and damage of the alveolar architecture. The LPS-induced neutrophil accumulation and the associated lung damage were enhanced in TDAG8-deficient mice as compared with those in wild-type mice. LPS also increased several mRNA and protein expressions of inflammatory cytokines and chemokines in the lungs or BAL fluids. Among these inflammatory mediators, mRNA and protein expression of KC (also known as CXCL1), a chemokine of neutrophils, were significantly enhanced by TDAG8 deficiency. We conclude that TDAG8 is a negative regulator for lung neutrophilic inflammation and injury, in part, through the inhibition of chemokine production.
authors.affiliation: Laboratory of Signal Transduction, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi 371-8512, Japan. m12702056@gunma-u.ac.jp.|Department of Medicine and Molecular Science, Gunma University Graduate School of Medicine, Maebashi 371-8511, Japan. m12702056@gunma-u.ac.jp.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Tsurumaki
authors.affiliation: Laboratory of Signal Transduction, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi 371-8512, Japan. cmogi@gunma-u.ac.jp.
authors.forename: Chihiro
authors.initials: C
authors.lastname: Mogi
authors.affiliation: Laboratory of Signal Transduction, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi 371-8512, Japan. a-haruka@gunma-u.ac.jp.
authors.forename: Haruka
authors.initials: H
authors.lastname: Aoki-Saito
authors.affiliation: Laboratory of Signal Transduction, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi 371-8512, Japan. mtobo@gunma-u.ac.jp.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Tobo
authors.affiliation: Department of Medicine and Molecular Science, Gunma University Graduate School of Medicine, Maebashi 371-8511, Japan. m08702012@gunma-u.ac.jp.
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Kamide
authors.affiliation: Department of Medicine and Molecular Science, Gunma University Graduate School of Medicine, Maebashi 371-8511, Japan. m09702007@gunma-u.ac.jp.
authors.forename: Masakiyo
authors.initials: M
authors.lastname: Yatomi
authors.affiliation: Laboratory of Signal Transduction, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi 371-8512, Japan. kosato@gunma-u.ac.jp.
authors.forename: Koichi
authors.initials: K
authors.lastname: Sato
authors.affiliation: Gunma University Graduate School of Health Sciences, Maebashi 371-8514, Japan. dobashik@gunma-u.ac.jp.
authors.forename: Kunio
authors.initials: K
authors.lastname: Dobashi
authors.affiliation: Third Department of Internal Medicine, Faculty of Medical Sciences, University of Fukui, Fukui 910-1193, Japan. tamotsui@u-fukui.ac.jp.
authors.forename: Tamotsu
authors.initials: T
authors.lastname: Ishizuka
authors.affiliation: Department of Medicine and Molecular Science, Gunma University Graduate School of Medicine, Maebashi 371-8511, Japan. hisadat@gunma-u.ac.jp.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Hisada
authors.affiliation: Department of Medicine and Molecular Science, Gunma University Graduate School of Medicine, Maebashi 371-8511, Japan. myamada@gunma-u.ac.jp.
authors.forename: Masanobu
authors.initials: M
authors.lastname: Yamada
authors.affiliation: Laboratory of Signal Transduction, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi 371-8512, Japan. okajima@gunma-u.ac.jp.
authors.forename: Fumikazu
authors.initials: F
authors.lastname: Okajima
mesh_terms: D055371:Acute Lung Injury / Q000235:genetics / Q000276:immunology* / Q000473:pathology*; D000818:Animals; D018925:Chemokines / Q000276:immunology; D005260:Female; D017353:Gene Deletion; D008070:Lipopolysaccharides / Q000276:immunology; D008168:Lung / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009504:Neutrophils / Q000276:immunology / Q000473:pathology; D011522:Protons; D012333:RNA, Messenger / Q000235:genetics; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000276:immunology; D015854:Up-Regulation
chemical_list: D018925:Chemokines; C513337:GPR65 protein, mouse; D008070:Lipopolysaccharides; D011522:Protons; D012333:RNA, Messenger; D043562:Receptors, G-Protein-Coupled
keywords: KC; TDAG8; acute lung injury; lipopolysaccharide; neutrophil