title: The Safety Evaluation of Salvianolic Acid B and Ginsenoside Rg1 Combination on Mice.
abstract: Our previous study indicated that the combination of salvianolic acid B (SalB) and ginsenoside Rg1 (Rg1), the main components of Salvia miltiorrhizae and Panax notoginseng, improves myocardium structure and ventricular function in rats with ischemia/reperfusion injury. The present study aimed to determine the safety of the combined SalB and Rg1 (SalB-Rg1) in mice. The safety of SalB-Rg1 was evaluated through acute toxicity and repeated-dose toxicity. In the acute toxicity study, the up and down procedure was carried out firstly, and then, the Bliss method was applied. In the toxicity study for seven-day repeated treatment of SalB-Rg1, forty Kunming mice were randomly divided into four groups. The intravenous median lethal dose (LD50) of the SalB-Rg1 combination was 1747 mg/kg using the Bliss method. For both the acute toxicity study and the seven-day repeated toxicity study, SalB-Rg1 did not induce significant abnormality on brain, heart, kidney, liver and lung structure at any dose based on H&amp;E stain. There were no significant changes related to the SalB-Rg1 toxicity detected on biochemical parameters for two kinds of toxicity studies. The LD50 in mice was 1747 mg/kg, which was more than one hundred times higher than the effective dose. Both studies of acute toxicity and seven-day repeated dose toxicity indicated the safety of the SalB-Rg1 combination.
authors.affiliation: Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Haike Road #501, Shanghai 201203, China. zhaoqun886@163.com.|Shenyang Pharmaceutical University, Wenhua Road #103, Shenyang 110016, China. zhaoqun886@163.com.
authors.forename: Qun
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Haike Road #501, Shanghai 201203, China. m_yang@simm.ac.cn.
authors.forename: Min
authors.initials: M
authors.lastname: Yang
authors.affiliation: Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Haike Road #501, Shanghai 201203, China. shimbiro@sina.com.
authors.forename: Yanping
authors.initials: Y
authors.lastname: Deng
authors.affiliation: Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Haike Road #501, Shanghai 201203, China. yuhaitaojerry@163.com.
authors.forename: Haitao
authors.initials: H
authors.lastname: Yu
authors.affiliation: Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Haike Road #501, Shanghai 201203, China. hhxxwll@163.com.
authors.forename: Linlin
authors.initials: L
authors.lastname: Wang
authors.affiliation: Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Haike Road #501, Shanghai 201203, China. fineskypig1983@163.com.|Shenyang Pharmaceutical University, Wenhua Road #103, Shenyang 110016, China. fineskypig1983@163.com.
authors.forename: Fukang
authors.initials: F
authors.lastname: Teng
authors.affiliation: Takarazuka University of Medical and Health Care, Hanayashiki-Midorigaoka, Takarazuka-City 6660162, Japan. duoli02@icloud.com.
authors.forename: Kenka
authors.initials: K
authors.lastname: Cho
authors.affiliation: East China University of Science and Technology Shanghai, Meilong Road 130, Shanghai 200237, China. hmma@ecust.edu.cn.
authors.forename: Hongmei
authors.initials: H
authors.lastname: Ma
authors.affiliation: Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Haike Road #501, Shanghai 201203, China. wp7665@sina.com.|Shenyang Pharmaceutical University, Wenhua Road #103, Shenyang 110016, China. wp7665@sina.com.
authors.forename: Peng
authors.initials: P
authors.lastname: Wu
authors.affiliation: Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Haike Road #501, Shanghai 201203, China. shirley_lixue22@163.com.
authors.forename: Xue
authors.initials: X
authors.lastname: Li
authors.affiliation: Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Haike Road #501, Shanghai 201203, China. wanyingwu@simm.ac.cn.
authors.forename: Wanying
authors.initials: W
authors.lastname: Wu
authors.affiliation: Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Haike Road #501, Shanghai 201203, China. xuanliu@simm.ac.cn.
authors.forename: Xuan
authors.initials: X
authors.lastname: Liu
authors.affiliation: Shenyang Pharmaceutical University, Wenhua Road #103, Shenyang 110016, China. xufengyaoli@sina.com.
authors.forename: Feng
authors.initials: F
authors.lastname: Xu
authors.affiliation: Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Haike Road #501, Shanghai 201203, China. jiangbh@simm.ac.cn.
authors.forename: Baohong
authors.initials: B
authors.lastname: Jiang
authors.affiliation: Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Haike Road #501, Shanghai 201203, China. daguo@simm.ac.cn.
authors.forename: De-An
authors.initials: DA
authors.lastname: Guo
mesh_terms: D000818:Animals; D001572:Benzofurans / Q000633:toxicity*; D002317:Cardiovascular Agents / Q000633:toxicity*; D004338:Drug Combinations; D004353:Drug Evaluation, Preclinical; D005260:Female; D036145:Ginsenosides / Q000633:toxicity*; D007928:Lethal Dose 50; D008297:Male; D051379:Mice; D053630:Depsides
chemical_list: D001572:Benzofurans; D002317:Cardiovascular Agents; D004338:Drug Combinations; D036145:Ginsenosides; C076944:salvianolic acid B; D053630:Depsides; C035054:ginsenoside Rg1
keywords: acute toxicity; ginsenoside Rg1; repeated toxicity; safety; salvianolic acid B