title: The effect of erythrocyte membranes from diabetic and hypercholesterolemic individuals on human carbonic anhydrase II activity.
abstract: BACKGROUND
  Erythrocyte membranes regulate many enzyme activities, including carbonic anhydrase II (CA II). Membrane fluidity is associated with alterations in protein function and protein-protein interactions.
  
  
  OBJECTIVE
  The purpose of this study was to show the human CA II (hCA II) activity regulation by human erythrocyte membranes from diabetic and hypercholesterolemic subjects.
  
  
  METHODS
  Erythrocyte membranes were obtained from diabetic, hypercholesterolemic, and healthy subjects. hCA II activity was measured using the electrometric method.
  
  
  RESULTS
  hCA II activity was increased in vitro by membranes from both diabetic and hypercholesterolemic patients, with hypercholesterolemic membranes exhibiting a greater increase.
  
  
  CONCLUSIONS
  Changes in membrane composition may affect the erythrocyte membranes' capacity to increase in vitro hCA II activity.
authors.affiliation: a Department of Biochemistry , Faculty of Pharmacy, Karadeniz Technical University , Trabzon , Turkey .
authors.forename: Sermet
authors.initials: S
authors.lastname: Yildirmis
authors.affiliation: b Department of Medical Biochemistry , Faculty of Medicine, Karadeniz Technical University , Trabzon , Turkey .
authors.forename: Ahmet
authors.initials: A
authors.lastname: Alver
authors.affiliation: c Department of Medical Services and Techniques , Vocational School of Health Services, Bülent Ecevit University , Zonguldak , Turkey , and.
authors.forename: Yunus Emre
authors.initials: YE
authors.lastname: Yandi
authors.affiliation: d Department of Nutrition and Dietetics , Faculty of Health Sciences, Karadeniz Technical University , Trabzon , Turkey.
authors.forename: Selim
authors.initials: S
authors.lastname: Demir
authors.affiliation: b Department of Medical Biochemistry , Faculty of Medicine, Karadeniz Technical University , Trabzon , Turkey .
authors.forename: Ayse
authors.initials: A
authors.lastname: Senturk
authors.affiliation: b Department of Medical Biochemistry , Faculty of Medicine, Karadeniz Technical University , Trabzon , Turkey .
authors.forename: Akin
authors.initials: A
authors.lastname: Bodur
authors.affiliation: b Department of Medical Biochemistry , Faculty of Medicine, Karadeniz Technical University , Trabzon , Turkey .
authors.forename: Ahmet
authors.initials: A
authors.lastname: Mentese
mesh_terms: D000328:Adult; D024402:Carbonic Anhydrase II / Q000378:metabolism*; D016022:Case-Control Studies; D003924:Diabetes Mellitus, Type 2 / Q000097:blood* / Q000201:enzymology*; D004910:Erythrocyte Membrane / Q000378:metabolism*; D005260:Female; D006801:Humans; D006937:Hypercholesterolemia / Q000097:blood* / Q000201:enzymology*; D008297:Male
chemical_list: D024402:Carbonic Anhydrase II
keywords: Erythrocyte membrane; human carbonic anhydrase II; hypercholesterolemia; type 2 diabetes