title: Zebrafish Whole-Mount In Situ Hybridization Followed by Sectioning.
abstract: In situ hybridization is a powerful technique used for locating specific nucleic acid targets within morphologically preserved tissues and cell preparations. A labeled RNA or DNA probe hybridizes to its complementary mRNA or DNA sequence within a sample. Here, we describe RNA in situ hybridization protocol for whole-mount zebrafish embryos.
authors.affiliation: Smith Cardiovascular Research Institute, University of California, San Francisco, CA, 94158-9001, USA.
authors.forename: Canan
authors.initials: C
authors.lastname: Doganli
authors.affiliation: Stereology and Electron Microscopy Laboratory, Centre for Stochastic Geometry and Advanced Bioimaging, Aarhus University Hospital, Aarhus University, Aarhus C, Denmark.
authors.forename: Jens Randel
authors.initials: JR
authors.lastname: Nyengaard
authors.affiliation: Department of Biomedicine and Centre for Membrane Pumps in Cells and Disease - PUMPKIN, Danish National Research Foundation, Aarhus University, Aarhus C, Denmark. kly@biomed.au.dk.|Aarhus Institute of Advanced Studies (AIAS), Aarhus University, Aarhus C, Denmark. kly@biomed.au.dk.
authors.forename: Karin
authors.initials: K
authors.lastname: Lykke-Hartmann
mesh_terms: D000818:Animals; D004625:Embryo, Nonmammalian / Q000378:metabolism; D005260:Female; D017403:In Situ Hybridization / Q000379:methods*; D015347:RNA Probes / Q000235:genetics; D013194:Staining and Labeling / Q000379:methods*; D015027:Zebrafish / Q000196:embryology*
chemical_list: D015347:RNA Probes
keywords: DIG-labeled RNA antisense probe; In situ hybridization; NBT/BCIP staining; Pre-hybridization; Sectioning; Zebrafish