title: First identification of Sarcocystis hominis in Iranian traditional hamburger.
abstract: Zoonotic concerns of cattle sarcocystosis are of importance, because humans are the final host for Sarcocystis hominis. Therefore the meat products containing beef may encompass sarcocysts which endanger food safety. In this study, we described the first report of molecular identification of S. hominis in Iranian traditional hamburgers using PCR-RFLP. Throughout a pilot research that was carried out to setup a molecular approach to identify the Sarcocystis spp., using PCR-RFLP, a sample of raw Iranian traditional hamburger was purchased from a street food seller located in Yazd, central Iran in May 2013. DNA extraction was done, by salting out method; briefly, the sample was lysed with NET buffer. The DNA purification and precipitation was then performed. Amplicon and digestion results were analyzed, using gel agarose electrophoresis. The results showed a PCR product with 926 bp in length after amplification and 376 and 550 bp in length after digestion. This product was identified as S. hominis. To the best of our knowledge, this is the first report of S. hominis infection in Iranian hamburger.
authors.affiliation: Department of Food Hygiene and Safety, Faculty of Health, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
authors.forename: M Moghaddam
authors.initials: MM
authors.lastname: Ahmadi
authors.affiliation: Department of Food Hygiene and Safety, Faculty of Health, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
authors.forename: B
authors.initials: B
authors.lastname: Hajimohammadi
authors.affiliation: Department of Parasitology and Mycology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
authors.forename: G
authors.initials: G
authors.lastname: Eslami
authors.affiliation: Department of Pathology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
authors.forename: A
authors.initials: A
authors.lastname: Oryan
authors.affiliation: Department of Food Science and Technology, Science and Research Branch, Islamic Azad University, Yazd, Iran.
authors.forename: S A
authors.initials: SA
authors.lastname: Yasini Ardakani
authors.affiliation: Department of Food Hygiene and Safety, Faculty of Health, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
authors.forename: A
authors.initials: A
authors.lastname: Zohourtabar
authors.affiliation: Yazd Health Office, Yazd, Iran.
authors.forename: S
authors.initials: S
authors.lastname: Zare
keywords: Iranian hamburger; PCR-RFLP; S. hominis