title: Production of γ-cyclodextrin by Bacillus cereus cyclodextrin glycosyltransferase using extractive bioconversion in polymer-salt aqueous two-phase system.
abstract: Aqueous two-phase system (ATPS) extractive bioconversion provides a technique which integrates bioconversion and purification into a single step process. Extractive bioconversion of gamma-cyclodextrin (γ-CD) from soluble starch with cyclodextrin glycosyltransferase (CGTase, EC 2.4.1.19) enzyme derived from Bacillus cereus was evaluated using polyethylene glycol (PEG)/potassium phosphate based on ATPS. The optimum condition was attained in the ATPS constituted of 30.0% (w/w) PEG 3000 g/mol and 7.0% (w/w) potassium phosphate. A γ-CD concentration of 1.60 mg/mL with a 19% concentration ratio was recovered after 1 h bioconversion process. The γ-CD was mainly partitioned to the top phase (YT=81.88%), with CGTase partitioning in the salt-rich bottom phase (KCGTase=0.51). Repetitive batch processes of extractive bioconversion were successfully recycled three times, indicating that this is an environmental friendly and a cost saving technique for γ-CD production and purification.
authors.affiliation: Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Yu Kiat
authors.initials: YK
authors.lastname: Lin
authors.affiliation: Department of Chemical and Environmental Engineering, Faculty of Engineering, University of Nottingham Malaysia Campus, Jalan Broga, 43500 Semenyih, Selangor Darul Ehsan, Malaysia; Manufacturing and Industrial Processes Division, Faculty of Engineering, Centre for Food and Bioproduct Processing, University of Nottingham Malaysia Campus, Jalan Broga, Semenyih 43500, Selangor Darul Ehsan, Malaysia.
authors.forename: Pau Loke
authors.initials: PL
authors.lastname: Show
authors.affiliation: Department of Applied Mathematics, Faculty of Engineering, University of Nottingham Malaysia Campus, Jalan Broga, Semenyih 43500, Selangor Darul Ehsan, Malaysia.
authors.forename: Yee Jiun
authors.initials: YJ
authors.lastname: Yap
authors.affiliation: Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
authors.forename: Arbakariya B
authors.initials: AB
authors.lastname: Ariff
authors.affiliation: Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Mohammad Suffian
authors.initials: MS
authors.lastname: Mohammad Annuar
authors.affiliation: Department of Bioprocess Technology, Faculty of Biotechnology and Bimolecular Sciences, University Putra Malaysia, 43400 Serdang, Malaysia.
authors.forename: Oi Ming
authors.initials: OM
authors.lastname: Lai
authors.affiliation: Department of Bioprocess Technology, Faculty of Biotechnology and Bimolecular Sciences, University Putra Malaysia, 43400 Serdang, Malaysia.
authors.forename: Teck Kim
authors.initials: TK
authors.lastname: Tang
authors.affiliation: Nanotechnology & Catalyst Research Centre (NANOCAT), University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Joon Ching
authors.initials: JC
authors.lastname: Juan
authors.affiliation: Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia. Electronic address: tcling@um.edu.my.
authors.forename: Tau Chuan
authors.initials: TC
authors.lastname: Ling
mesh_terms: D001409:Bacillus cereus / Q000201:enzymology*; D005964:Glucosyltransferases / Q000378:metabolism*; D010710:Phosphates / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D017680:Potassium Compounds / Q000737:chemistry*; D013213:Starch / Q000737:chemistry / Q000378:metabolism; D014867:Water / Q000737:chemistry*; D047408:gamma-Cyclodextrins / Q000302:isolation & purification* / Q000378:metabolism*
chemical_list: D010710:Phosphates; D017680:Potassium Compounds; D047408:gamma-Cyclodextrins; D014867:Water; D011092:Polyethylene Glycols; D013213:Starch; C013216:potassium phosphate; D005964:Glucosyltransferases; C019963:cyclomaltodextrin glucanotransferase; C023792:gamma-cyclodextrin
keywords: Aqueous two-phase system; Bacillus cereus; Cyclodextrin; Cyclodextrin glycosyltransferease; Extractive bioconversion