title: Seco-limonoid derived from Raputia heptaphylla promotes the control of cutaneous leishmaniasis in hamsters (Mesocricetus auratus).
abstract: The rational search of novel bioactive molecules against pathogens with immunomodulatory activity is presently one of the most significant approaches to discover and design new therapeutic agents for effective control of infectious diseases, such as the infection caused by Leishmania parasites. In the present study, we evaluated the therapeutic efficacy of the recently characterized immunomodulatory compound 11α,19β-dihydroxy-7-acetoxy-7-deoxoichangin, a seco-limonoid derived from the bark of Raputia heptaphylla (Pittier) using: (1) peritoneal macrophages and (2) Mesocricetus auratus hamsters infected with Leishmania (V.) panamensis and Leishmania (L.) amazonensis. We observed the ability of this seco-limonoid to induce the effective control of the parasite either in vitro [determining an effective concentration 50 (EC50) of 59 µ m at the infection model] and in vivo (inducing clinical improvement or even cure in infected animals treated compared with the groups of animals treated with vehicle solution or meglumine antimoniate).
authors.affiliation: Research Group in Immunotoxicology,Faculty of Sciences,Universidad Nacional de Colombia,Bogota 11001,Colombia.
authors.forename: Diana
authors.initials: D
authors.lastname: Granados-Falla
authors.affiliation: Research Group in Immunotoxicology,Faculty of Sciences,Universidad Nacional de Colombia,Bogota 11001,Colombia.
authors.forename: Angela
authors.initials: A
authors.lastname: Gomez-Galindo
authors.affiliation: PECET - Medical Research Institute,School of Medicine,Universidad de Antioquia,Medellin 50010,Colombia.
authors.forename: Alejandro
authors.initials: A
authors.lastname: Daza
authors.affiliation: PECET - Medical Research Institute,School of Medicine,Universidad de Antioquia,Medellin 50010,Colombia.
authors.forename: Sara
authors.initials: S
authors.lastname: Robledo
authors.affiliation: Research Laboratory of Natural Plant Products,Faculty of Sciences,Universidad Nacional de Colombia,Bogota 11001,Colombia.
authors.forename: Carlos
authors.initials: C
authors.lastname: Coy-Barrera
authors.affiliation: Research Laboratory of Natural Plant Products,Faculty of Sciences,Universidad Nacional de Colombia,Bogota 11001,Colombia.
authors.forename: Luis
authors.initials: L
authors.lastname: Cuca
authors.affiliation: Research Group in Immunotoxicology,Faculty of Sciences,Universidad Nacional de Colombia,Bogota 11001,Colombia.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Delgado
mesh_terms: D000818:Animals; D000981:Antiprotozoal Agents / Q000494:pharmacology / Q000627:therapeutic use; D006224:Cricetinae; D005260:Female; D007891:Leishmania / Q000187:drug effects; D016773:Leishmaniasis, Cutaneous / Q000188:drug therapy*; D036701:Limonins / Q000494:pharmacology / Q000627:therapeutic use*; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000469:parasitology; D008297:Male; D008647:Mesocricetus; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D027821:Rutaceae / Q000737:chemistry*; D016896:Treatment Outcome
chemical_list: D000981:Antiprotozoal Agents; D036701:Limonins; D010936:Plant Extracts
keywords:  seco-limonoid; Leishmania; hamsters; natural products; treatment