title: Detection of p16(INK4A) in the Mixed Cell Populations of Normal Peripheral Blood Mononuclear Cells and Cervical Cancer Cell Lines.
abstract: OBJECTIVE
  Human papilloma viruses (HPVs) play a central role in the pathogenesis of neoplastic lesions of the uterine cervix. The viral oncoprotein HPV E6 degrades the p53 protein, and the HPV E7 protein inactivates pRB and increases the expression of the CDK inhibitor, p16(INK4A). We investigated the usefulness of p16(INK4A) as a biologic marker for the cervical dysplastic and neoplastic cells.
  
  
  METHODS
  We examined the expression of p16(INK4A) and cytokeratin in a mixed population of normal peripheral blood mononuclear cells (PBMC) and the cervical cancer cell lines (HeLa, SiHa, and CasKi) using flow cytometry.
  
  
  RESULTS
  The DNA indices of the HeLa, SiHa and CasKi cell lines were 1.89, 1.53 and 1.75, respectively, indicating that these cells are aneuploid cells. Furthermore, the positive rate of p16(INK4A) expression was 86.7% for the HeLa mixed population, 85.6% for the SiHa mixed population, and 92.2% for the CasKi mixed population. According to the FL3A vs FL3W histogram, electrical gating of the HeLa, SiHa and CasKi mixed populations showed the expression levels of both cytokeratin and p16(INK4A) to be identical, at 86.6%, 84.8% and 85.0%, respectively. These findings revealed that almost all cells selected through electrical gating were cervical cancer cells originating from the epithelium and which expressed cytokeratin and p16(INK4A). On the other hand, when each mixed population was electrically gated for normal PBMC, we found that the PBMCs expressed neither cytokeratin nor p16(INK4A).
  
  
  CONCLUSIONS
  Using flow cytometry, we observed the enhanced expression of p16(INK4A) in cervical cancer cell lines. These.
  
  
  RESULTS
  suggest the usefulness of p16(INK4A) for the selective detection of cervical dysplastic and cancer cells in the liquid-based samples, which are taken from the cervices and contaminated with blood and stromal cells.
authors.forename: Ji Young
authors.initials: JY
authors.lastname: Kwon
authors.forename: Yoon Sung
authors.initials: YS
authors.lastname: Jo
authors.forename: Ye Hoon
authors.initials: YH
authors.lastname: Choi
authors.forename: Jong Gyu
authors.initials: JG
authors.lastname: Chang
authors.forename: Ki Sung
authors.initials: KS
authors.lastname: Ryu
authors.forename: Jong Gu
authors.initials: JG
authors.lastname: Rha
authors.forename: Ku Taek
authors.initials: KT
authors.lastname: Han
keywords: Cervical cancer cell lines; Cytokeratin; Flow cytometry; p16(INK4A)