title: Study of genetic variation of eggplant cultivars by using RAPD-PCR molecular markers and the relationship with Phomopsis blight disease reaction.
abstract: Disease susceptibility and genetic variability in 10 eggplant genotypes were studied after inoculating Phomopsis vexans under confined field conditions. Random amplified polymorphic DNA (RAPD) markers were used to assess genetic variation and relationships among eggplant genotypes. The disease index of leaves ranged 0.208-13.79%, while fruit infection ranged 2.15-42.76%. Two varieties, Dohazari G and Laffa S, were found to be susceptible, 6 were moderately resistant, 1 was moderately susceptible, and BAU Begun-1 was resistant to P. vexans. Amplification of genomic DNA by using 3 RAPD primers produced 20 bands: 14 (70%) were polymorphic and 6 (30%) were monomorphic. The highest intra-variety similarity indices values were found in ISD 006, Ishurdi L, Jessore L, and BAU Begun-1 (100%), while the lowest was in Dohazari G (90%). The lowest genetic distance (0.0513) and the highest genetic identity (0.9500) were observed between the ISD 006 and Ishurdi L combinations. A comparatively higher genetic distance (0.3724) and the lowest genetic identity (0.6891) were observed between the ISD 006 and Dohazari G combinations. A dendogram was constructed based on Nei's genetic distance, which produced 2 main clusters of the genotypes - Cluster I: ISD 006, Ishurdi L, Marich begun L, BAU Begun-1, Marich begun S, and Chega and Cluster 2: Laffa S, Dohazari G, Jessore L, and Singhnath. Genetic variation and its relationship with disease susceptibility were assessed using RAPD markers, to develop disease-resistant varieties and improve eggplant crops.
authors.affiliation: Department of Plant Pathology, Bangladesh Agricultural University, Mymensingh, Bangladesh.
authors.forename: H A
authors.initials: HA
authors.lastname: Asad
authors.affiliation: Department of Plant Pathology, Bangladesh Agricultural University, Mymensingh, Bangladesh.
authors.forename: M B
authors.initials: MB
authors.lastname: Meah
authors.affiliation: Bangladesh Institute of Nuclear Agriculture, Mymensingh, Bangladesh.
authors.forename: S N
authors.initials: SN
authors.lastname: Begum
authors.affiliation: Bangladesh Institute of Nuclear Agriculture, Mymensingh, Bangladesh.
authors.forename: M I
authors.initials: MI
authors.lastname: Khalil
authors.affiliation: Institute of Tropical Agriculture, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.|Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
authors.forename: M Y
authors.initials: MY
authors.lastname: Rafii
authors.affiliation: Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.|Plant Pathology Division, Bangladesh Rice Research Institute, Gazipur, Bangladesh.
authors.forename: M A
authors.initials: MA
authors.lastname: Latif
mesh_terms: D001203:Ascomycota; D060467:Disease Resistance / Q000235:genetics; D019143:Evolution, Molecular; D005819:Genetic Markers; D020022:Genetic Predisposition to Disease; D014644:Genetic Variation; D010802:Phylogeny; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology; D016133:Polymerase Chain Reaction; D019105:Random Amplified Polymorphic DNA Technique; D032323:Solanum melongena / Q000145:classification / Q000235:genetics*
chemical_list: D005819:Genetic Markers