title: A systematic study of nuclear interactome of C-terminal domain small phosphatase-like 2 using inducible expression system and shotgun proteomics.
abstract: RNA polymerase II C-terminal domain phosphatases are newly emerging family of phosphatases that contain FCPH domain with Mg＋2-binding DXDX(T/V) signature motif. Its subfamily includes small CTD phosphatases (SCPs). Recently, we identified several interacting partners of human SCP1 with appearance of dephosphorylation and O-GlcNAcylation. In this study, using an established cell line with inducible CTDSPL2 protein (a member of the new phosphatase family), proteomic screening was conducted to identify binding partners of CTDSPL2 in nuclear extract through immunoprecipitation of CTDSPL2 with its associated. This approach led to the identification of several interacting partners of CTDSPL2. This will provide a better understanding on CTDSPL2. [BMB Reports 2016; 49(6): 319-324].
authors.affiliation: Department of Brain & Cognitive Sciences, DGIST, Daegu 42988, Korea.
authors.forename: NaNa
authors.initials: N
authors.lastname: Kang
authors.affiliation: Department of Brain & Cognitive Sciences, DGIST, Daegu 42988, Korea.
authors.forename: JaeHyung
authors.initials: J
authors.lastname: Koo
authors.affiliation: Qiqihar Medical University, Qiqihar City, Heilongjiang, 161006, China.
authors.forename: Sen
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Animal Science and Technology, Chung-Ang University, Anseong 17546, Korea.
authors.forename: Sun Jin
authors.initials: SJ
authors.lastname: Hur
authors.affiliation: Department of Biotechnology, Konkuk University, Chungju 27478, Korea.
authors.forename: Young Yil
authors.initials: YY
authors.lastname: Bahk
mesh_terms: D002467:Cell Nucleus / Q000378:metabolism*; D002846:Chromatography, Affinity; D057809:HEK293 Cells; D006801:Humans; D047468:Immunoprecipitation; D010749:Phosphoprotein Phosphatases / Q000378:metabolism*; D011485:Protein Binding; D025941:Protein Interaction Mapping; D040901:Proteomics / Q000379:methods*; D015203:Reproducibility of Results; D013347:Subcellular Fractions / Q000378:metabolism
chemical_list: D010749:Phosphoprotein Phosphatases; C116022:carboxy-terminal domain phosphatase