title: Zeta potential response of human erythrocyte membranes to the modulators of Gardos channel activity under low rate β-radiation.
abstract: OBJECTIVE
  Study of human erythrocyte DP response under modification by activators and blockers of the functional state of Ca2+-dependent K+ channels under low rate β-radiation.
  
  
  METHODS
  Erythrocytes were isolated from the donor blood. The zeta potential was computed from the value of the cell electrophoretic mobility. The investigated drugs preliminary introduced in cellular suspensions, and then aliquote of 90Sr(NO3)2 solution to get the final activity concentration of 44,4kBq⋅l-1.
  
  
  RESULTS
  The radioisotope radiation of 90Sr/90Y (RR, 15 μGy⋅h-1) increases an absolute value of erythrocyte membranes DP (DPab), and its action is reversible. It specifies the effect is mediated by non-ionizing part of the RR. Dibutyril-cAMP dose-independent increases DPab of erythrocyte membranes in the concentration range of 1-100 мкМ, but RR does not amplify this effect. Anaprilin increases dose-independent DPab in concentrations 10 and 100 μМ. The effect of maximal concentration of anaprilin (100 μМ) decreases by RR. Clotrimazol increases DPab of erythrocyte membranes in the concentration range of 0,1-10 μМ relatively control, while its maximal concentration - decreases, and the minimal level does not reliably influence on this index The action of сlotrimazol on DP in concentrations of 10-100 μМ is abolished by RR, and is not changed in the range of 0,1-1,0 μМ. Nitrendipine raises DPab of erythrocyte membranes in all of range of concentrations, and RR amplifies the effect of the drug.
  
  
  CONCLUSIONS
  1. There is a threshold of the biological action on cells for the ionizing component of radioisotope radiation determined by efficiency of operation their antioxidant system.2. At dose rates below a threshold, the action of ionizing radiation is mediated by its non-ionizing component, and is reversible, and therefore is determined only in the field of radiation.
authors.affiliation: Institute of Bioorganic Chemistry and Petrochemistry of National Academy of Sciences of Ukraine., 1 Murmanska St., Kyiv, 02660, Ukraine.
authors.forename: V V
authors.initials: VV
authors.lastname: Zhirnov
authors.affiliation: Institute of Bioorganic Chemistry and Petrochemistry of National Academy of Sciences of Ukraine., 1 Murmanska St., Kyiv, 02660, Ukraine.
authors.forename: I N
authors.initials: IN
authors.lastname: Iakovenko
authors.affiliation: National University of Life and Environmental Sciences of Ukraine, 15 Heroyiv Oborony St., Kyiv, 03041, Ukraine.
authors.forename: V M
authors.initials: VM
authors.lastname: Voitsitskiy
authors.affiliation: National University of Life and Environmental Sciences of Ukraine, 15 Heroyiv Oborony St., Kyiv, 03041, Ukraine.
authors.forename: S V
authors.initials: SV
authors.lastname: Khyzhnyak
authors.affiliation: Public institution Institute of Heart of the Ministry of Health of Ukraine, 5A Bratyslavska St., Kyiv, 02660, Ukraine.
authors.forename: O G
authors.initials: OG
authors.lastname: Zubrikova-Chugainova
authors.affiliation: Public institution Institute of Heart of the Ministry of Health of Ukraine, 5A Bratyslavska St., Kyiv, 02660, Ukraine.
authors.forename: V A
authors.initials: VA
authors.lastname: Gorobetz
keywords: Gardos channel; biomembranes; human erythrocytes; strontium-90; zeta potential; β-radiation