title: The evaluation of loop-mediated isothermal amplification-quartz crystal microbalance (LAMP-QCM) biosensor as a real-time measurement of HPV16 DNA.
abstract: We have previously developed quartz crystal microbalance biosensor integrated with loop-mediated isothermal amplification (LAMP-QCM) for human papillomavirus (HPV) type58 DNA detection. Infection with HPV, particularly HPV16, remains a serious health problem due to its major risk factor contributing to cervical cancer. In the present study, LAMP-QCM biosensor was evaluated in terms of a quantitative assay for copy number of HPV16 DNA in cervical samples compared to quantitative PCR using TaqMan assay (TaqMan-qPCR). The detection limit of LAMP-QCM was found to be 10 fold more sensitive than TaqMan-qPCR with 100% specificity and 7.6% imprecision. Different plot of HPV16 DNA copy number using Bland-Altman analysis revealed 94% correlation between LAMP-QCM and qPCR. We therefore concluded that the developed LAMP-QCM biosensor provides a possible rapid and sensitive assay for HPV16 DNA quantification in a routine laboratory.
authors.affiliation: Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand. Electronic address: patjea@kku.ac.th.
authors.forename: Patcharee
authors.initials: P
authors.lastname: Jearanaikoon
authors.affiliation: College of Medicine and Public Health, Ubon Ratchathani University, Ubon Ratchathani 34190, Thailand.
authors.forename: Preeda
authors.initials: P
authors.lastname: Prakrankamanant
authors.affiliation: Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand.
authors.forename: Chanvit
authors.initials: C
authors.lastname: Leelayuwat
authors.affiliation: College of Medicine and Public Health, Ubon Ratchathani University, Ubon Ratchathani 34190, Thailand.
authors.forename: Surasak
authors.initials: S
authors.lastname: Wanram
authors.affiliation: Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand.
authors.forename: Temduang
authors.initials: T
authors.lastname: Limpaiboon
authors.affiliation: Department of Biomedical Engineering, Faculty of Engineering, Mahidol University, Nakhon Pathom 73170, Thailand.
authors.forename: Chamras
authors.initials: C
authors.lastname: Promptmas
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D002584:Cervix Uteri / Q000821:virology; D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics; D005260:Female; D052162:Human papillomavirus 16 / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D030361:Papillomavirus Infections / Q000175:diagnosis* / Q000821:virology; D058525:Quartz Crystal Microbalance Techniques / Q000379:methods*; D012680:Sensitivity and Specificity; D013997:Time Factors; D019562:Viral Load / Q000379:methods*
chemical_list: D004279:DNA, Viral
keywords: Cervical cancer; HPV16 DNA measurement; Loop-mediated isothermal amplification; Probe-free biosensor; Quartz crystal microbalance; TaqMan-qPCR