title: Atrazine and malathion shorten the maturation process of Xenopus laevis oocytes and have an adverse effect on early embryo development.
abstract: The use of pesticides has a negative impact on the environment. Amphibians have long been regarded as indicator species to pollutants due to their permeable skin and sensitivity to the environment. Studies have shown that population declines of some amphibians are directly linked with exposure to agricultural contaminants. In the past, much of the studies have focused on the toxic effect of contaminants on larvae (tadpoles), juvenile and adult frogs. However, due to the nature of their life cycle, amphibian eggs and early embryos are especially susceptible to the contaminants, and any alteration during the early reproductive stages may have a profound effect on the health and population of amphibians. In this study, we analyzed the effect of atrazine and malathion, two commonly used pesticides, on Xenopus laevis oocyte maturation and early embryogenesis. We found that both atrazine and malathion shortened the frog oocyte maturation process and resulted in reduced Emi2 levels at cytostatic factor-mediated metaphase arrest, and a high level of Emi2 is critically important for oocyte maturation. Furthermore, frog embryos fertilized under the influence of atrazine and/or malathion displayed a higher rate of abnormal division that eventually led to embryo death during early embryogenesis.
authors.affiliation: Department of Biological Sciences, California State Polytechnic University, Pomona, 3801 W. Temple Ave., Pomona, CA 91768, USA.
authors.forename: Qichao
authors.initials: Q
authors.lastname: Ji
authors.affiliation: Department of Biological Sciences, California State Polytechnic University, Pomona, 3801 W. Temple Ave., Pomona, CA 91768, USA.
authors.forename: Jessica
authors.initials: J
authors.lastname: Lee
authors.affiliation: Department of Biological Sciences, California State Polytechnic University, Pomona, 3801 W. Temple Ave., Pomona, CA 91768, USA.
authors.forename: Yu-Huey
authors.initials: YH
authors.lastname: Lin
authors.affiliation: Department of Biological Sciences, California State Polytechnic University, Pomona, 3801 W. Temple Ave., Pomona, CA 91768, USA.
authors.forename: Guihua
authors.initials: G
authors.lastname: Jing
authors.affiliation: Department of Biological Sciences, California State Polytechnic University, Pomona, 3801 W. Temple Ave., Pomona, CA 91768, USA.
authors.forename: L Jillianne
authors.initials: LJ
authors.lastname: Tsai
authors.affiliation: Department of Biological Sciences, California State Polytechnic University, Pomona, 3801 W. Temple Ave., Pomona, CA 91768, USA.
authors.forename: Andrew
authors.initials: A
authors.lastname: Chen
authors.affiliation: Department of Biological Sciences, California State Polytechnic University, Pomona, 3801 W. Temple Ave., Pomona, CA 91768, USA.
authors.forename: Lindsay
authors.initials: L
authors.lastname: Hetrick
authors.affiliation: Department of Biological Sciences, California State Polytechnic University, Pomona, 3801 W. Temple Ave., Pomona, CA 91768, USA.
authors.forename: Dylan
authors.initials: D
authors.lastname: Jocoy
authors.affiliation: Department of Biological Sciences, California State Polytechnic University, Pomona, 3801 W. Temple Ave., Pomona, CA 91768, USA. Electronic address: junjunliu@cpp.edu.
authors.forename: Junjun
authors.initials: J
authors.lastname: Liu
mesh_terms: D000818:Animals; D001280:Atrazine / Q000633:toxicity*; D004625:Embryo, Nonmammalian / Q000187:drug effects; D047108:Embryonic Development / Q000187:drug effects*; D005260:Female; D006540:Herbicides / Q000633:toxicity*; D007306:Insecticides / Q000633:toxicity*; D008294:Malathion / Q000633:toxicity*; D009865:Oocytes / Q000187:drug effects; D014982:Xenopus laevis / Q000196:embryology*
chemical_list: D006540:Herbicides; D007306:Insecticides; D001280:Atrazine; D008294:Malathion
keywords: Atrazine; Embryo development; Malathion; Oocyte maturation; Pesticide; Xenopus