title: Novel silver nanoparticle-enhanced fluorometric determination of trace tetracyclines in aqueous solutions.
abstract: Metal-enhanced fluorescence (MEF) has exhibited promise for applications in fluorometric assays. The effects of silver nanoparticles (AgNP) on the fluorescence behaviours of tetracycline hydrochloride (TCH) and chlortetracycline hydrochloride (CTC) in aqueous solutions were investigated. The experimental results demonstrated that the fluorescence intensities of each tetracycline in water solutions were greatly enhanced by AgNP through the MEF effect. In addition, a novel silver nanoparticle-enhanced fluorometric method was established for the direct determination of TCH and CTC in aqueous solutions. Under optimum experimental conditions, the linear dynamic ranges for the determination of TCH and CTC in aqueous solutions varied from 0.10 to 6.0 mg L(-1) and 0.050 to 3.0 mg L(-1) with detection limits of 0.63 µg L(-1) and 0.19 µg L(-1), respectively, and with the relative standard deviation of less than 1.9% (n=9). The experimental recovery results for the determination of TCH and CTC in aqueous solutions ranged from 93-106% and 95-104%, respectively. Compared with the established method without the addition of AgNP, the limits of quantitation of the silver nanoparticle-enhanced fluorometric method were approximately 5-fold lower for TCH and 3-fold lower for CTC. Moreover, the newly established silver nanoparticle-enhanced fluorometric method was successfully applied to the direct determination of TCH and CTC in pharmaceutical preparations.
authors.affiliation: School of Environmental Science and Public Health, Wenzhou Medical University, Wenzhou 325035, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.affiliation: School of Information and Engineering, Wenzhou Medical University, Wenzhou 325035, China.
authors.forename: Tun-Hua
authors.initials: TH
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Marine Environmental Science (Xiamen University), College of the Environment and Ecology, Xiamen University, Xiamen 361005, China. Electronic address: fruitful@xmu.edu.cn.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D002751:Chlortetracycline / Q000032:analysis*; D004339:Drug Compounding; D005470:Fluorometry / Q000379:methods*; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry*; D012834:Silver / Q000737:chemistry*; D012996:Solutions; D013752:Tetracycline / Q000032:analysis*; D014867:Water / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000032:analysis
chemical_list: D012996:Solutions; D014874:Water Pollutants, Chemical; D014867:Water; D012834:Silver; D013752:Tetracycline; D002751:Chlortetracycline
keywords: Chlortetracycline hydrochloride; Fluorometry; Metal-enhanced fluorescence; Silver nanoparticles; Tetracycline hydrochloride