title: [Optimization of extraction technology from Paeoniae Radix Alba using response surface methodology].
abstract: To ensure the stability of chemistry components and the convenience of operation, ultrasound method was chosen to study in this investigation. As the total common peaks area in chromatograms was set to be evaluation index, the influence on the technology caused by extraction time, ethanol concentration and liquid-to-solid ratio was studied by using single factor methodology, and the extraction technology of Paeoniae Radix Alba was optimized by using response surface methodology. The results showed that the extracting results were most affected by ethanol concentration; liquid-to-solid ratio came the second and extraction time thirdly. The optimum ultrasonic-assisted extraction conditions were as follow: the ultrasonic extraction time was 20.06 min, the ethanol concentration in solvent was 72.04%, and the liquid-to-solid ratio was 53.38 mL · g(-1), the predicted value of total common peaks area was 2.1608 x 10(8). Under the extraction conditions after optimization, the total common peaks area was 2.1422 x 10(8), and the relative deviation between the measured and predicted value was 0.86%, so the optimized extraction technology for Paeoniae Radix Alba is suitable and feasible. Besides, for the purpose of extracting more sufficiently and completely, the optimized extraction technology had more advantages than the extraction method recorded in the monogragh of Paeoniae Radix Alba in Chinese Pharmacopoeia, which will come true the assessment and utilization comprehensively.
authors.forename: Lin
authors.initials: L
authors.lastname: Jin
authors.forename: Wan-shun
authors.initials: WS
authors.lastname: Zhao
authors.forename: Qiao-sheng
authors.initials: QS
authors.lastname: Guo
authors.forename: Wen-sheng
authors.initials: WS
authors.lastname: Zhang
authors.forename: Zheng-liang
authors.initials: ZL
authors.lastname: Ye
mesh_terms: D029596:Paeonia / Q000737:chemistry*; D013678:Technology, Pharmaceutical / Q000379:methods*