title: Comparative Transcriptome Analysis of Genes Involved in GA-GID1-DELLA Regulatory Module in Symbiotic and Asymbiotic Seed Germination of Anoectochilus roxburghii (Wall.) Lindl. (Orchidaceae).
abstract: Anoectochilus roxburghii (Wall.) Lindl. (Orchidaceae) is an endangered medicinal plant in China, also called "King Medicine". Due to lacking of sufficient nutrients in dust-like seeds, orchid species depend on mycorrhizal fungi for seed germination in the wild. As part of a conservation plan for the species, research on seed germination is necessary. However, the molecular mechanism of seed germination and underlying orchid-fungus interactions during symbiotic germination are poorly understood. In this study, Illumina HiSeq 4000 transcriptome sequencing was performed to generate a substantial sequence dataset of germinating A. roxburghii seed. A mean of 44,214,845 clean reads were obtained from each sample. 173,781 unigenes with a mean length of 653 nt were obtained. A total of 51,514 (29.64%) sequences were annotated, among these, 49 unigenes encoding proteins involved in GA-GID1-DELLA regulatory module, including 31 unigenes involved in GA metabolism pathway, 5 unigenes encoding GID1, 11 unigenes for DELLA and 2 unigenes for GID2. A total of 11,881 genes showed significant differential expression in the symbiotic germinating seed sample compared with the asymbiotic germinating seed sample, of which six were involved in the GA-GID1-DELLA regulatory module, and suggested that they might be induced or suppressed by fungi. These results will help us understand better the molecular mechanism of orchid seed germination and orchid-fungus symbiosis.
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences &amp; Peking Union Medical College, Beijing 100193, China. liusisi274@126.com.
authors.forename: Si-Si
authors.initials: SS
authors.lastname: Liu
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences &amp; Peking Union Medical College, Beijing 100193, China. kibchenjuan@126.com.
authors.forename: Juan
authors.initials: J
authors.lastname: Chen
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences &amp; Peking Union Medical College, Beijing 100193, China. lixiaofei1117@126.com.
authors.forename: Shu-Chao
authors.initials: SC
authors.lastname: Li
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences &amp; Peking Union Medical College, Beijing 100193, China. zengxu1986@sina.com.
authors.forename: Xu
authors.initials: X
authors.lastname: Zeng
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences &amp; Peking Union Medical College, Beijing 100193, China. mengzhixianj@163.com.
authors.forename: Zhi-Xia
authors.initials: ZX
authors.lastname: Meng
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences &amp; Peking Union Medical College, Beijing 100193, China. sxguo1986@163.com.
authors.forename: Shun-Xing
authors.initials: SX
authors.lastname: Guo
mesh_terms: D030541:Databases, Genetic; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D063990:Gene Ontology; D053263:Gene Regulatory Networks; D017343:Genes, Plant; D018525:Germination / Q000235:genetics*; D058977:Molecular Sequence Annotation; D038821:Mycorrhizae; D029595:Orchidaceae / Q000235:genetics* / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D012639:Seeds / Q000235:genetics*; D013559:Symbiosis / Q000235:genetics*; D059467:Transcriptome / Q000235:genetics*
chemical_list: D010940:Plant Proteins
keywords: Anoectochilus roxburghii; GA-GID1-DELLA; Orchidaceae; asymbiotic; seed germination; symbiotic; transcriptome