title: Evolution of satellite DNA sequences in two tribes of Bovidae: A cautionary tale.
abstract: Two clones, Bt1 from Bos taurus and Om1 from Ovis orientalis musimon, were used as probes for hybridization on genomic DNA and on metaphase chromosomes in members of Bovini and Caprini tribes. Bt1 and Om1 are sequences respectively belonging to the 1.715 and 1.714 DNA satellite I families. Southern blots and fluorescence in situ hybridization experiments showed completely coherent results: the Bovini probe Bt1 hybridized only to members of the Bovini tribe and not to members of Caprini. Likewise, the Caprini probe Om1 hybridized only to members of the Caprini tribe and not to members of Bovini. Hybridization signals were detected in the heterochromatic regions of every acrocentric autosome, except for two pairs of autosomes from Capra hircus that did not show hybridization to probe Om1. No signal was detected on X and Y chromosomes or on bi-armed autosomes. Remarkably, probe Om1 showed almost 100% homology with a bacterial sequence reported in Helicobacter pylori.
authors.affiliation: Department of Biomedical Sciences, University of Cagliari, Monserrato, Italy.
authors.forename: Mariella
authors.initials: M
authors.lastname: Nieddu
authors.affiliation: Department of Biomedical Sciences, University of Cagliari, Monserrato, Italy.
authors.forename: Roberto
authors.initials: R
authors.lastname: Mezzanotte
authors.affiliation: Department of Surgical Sciences, University of Cagliari, Cagliari, Italy.
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Pichiri
authors.affiliation: Department of Surgical Sciences, University of Cagliari, Cagliari, Italy.
authors.forename: Pier Paolo
authors.initials: PP
authors.lastname: Coni
authors.affiliation: Department of Veterinary Medicine, University of Sassari, Sassari, Italy.
authors.forename: Gian Luca
authors.initials: GL
authors.lastname: Dedola
authors.affiliation: Department of Veterinary Medicine, University of Sassari, Sassari, Italy.
authors.forename: Maria Luisa
authors.initials: ML
authors.lastname: Dettori
authors.affiliation: Department of Veterinary Medicine, University of Sassari, Sassari, Italy.
authors.forename: Michele
authors.initials: M
authors.lastname: Pazzola
authors.affiliation: Department of Veterinary Medicine, University of Sassari, Sassari, Italy.
authors.forename: Giuseppe Massimo
authors.initials: GM
authors.lastname: Vacca
authors.affiliation: Department of Biomedical Sciences, University of Cagliari, Monserrato, Italy.
authors.forename: Renato
authors.initials: R
authors.lastname: Robledo