title: Effects and mechanism of dual-frequency power ultrasound on the molecular weight distribution of corn gluten meal hydrolysates.
abstract: The impact of dual-frequency power ultrasound (DPU) on the molecular weight distribution (MWD) of corn gluten meal (CGM) hydrolysates and its mechanism were investigated in the present study. The mechanism was studied from aspects of structural and nano-mechanical characteristics of the major protein fractions of CGM, viz. zein and glutelin. The results of molecular weight distribution indicated that DPU pretreatment of CGM was beneficial to the preparation of peptides with molecular weights of 200-1000Da. Moreover, FTIR spectral analysis and atomic force microscopy characterization showed that the DPU pretreatment changed the contents of secondary structure of proteins, decreased the particle height and surface roughness of glutelin, reduced the Young's modulus and stiffness of zein while increased its adhesion force. In conclusion, DPU pretreatment of proteins before proteolysis is an efficient alternative method to produce short-chain peptides because of its positive effects originating from acoustic cavitation on the molecular conformation, nano-structures and nano-mechanical properties of proteins as well.
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang, Jiangsu 212013, PR China; School of Life Science and Engineering, Southwest University of Science and Technology, 59 Qinglong Road, Mianyang, Sichuan 621000, PR China.
authors.forename: Jian
authors.initials: J
authors.lastname: Jin
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang, Jiangsu 212013, PR China; Jiangsu Provincial Key Laboratory for Physical Processing of Agricultural Products, Zhenjiang, Jiangsu 212013, PR China. Electronic address: mhl@ujs.edu.cn.
authors.forename: Haile
authors.initials: H
authors.lastname: Ma
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang, Jiangsu 212013, PR China.
authors.forename: Bei
authors.initials: B
authors.lastname: Wang
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang, Jiangsu 212013, PR China; Faulty of Agriculture, University of Zalingei, PO Box 6, Zalingei, Sudan.
authors.forename: Abu El-Gasim A
authors.initials: Ael-G
authors.lastname: Yagoub
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang, Jiangsu 212013, PR China.
authors.forename: Kai
authors.initials: K
authors.lastname: Wang
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang, Jiangsu 212013, PR China; Jiangsu Provincial Key Laboratory for Physical Processing of Agricultural Products, Zhenjiang, Jiangsu 212013, PR China.
authors.forename: Ronghai
authors.initials: R
authors.lastname: He
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang, Jiangsu 212013, PR China; Jiangsu Provincial Key Laboratory for Physical Processing of Agricultural Products, Zhenjiang, Jiangsu 212013, PR China.
authors.forename: Cunshan
authors.initials: C
authors.lastname: Zhou
mesh_terms: D005591:Chemical Fractionation; D005983:Glutens / Q000737:chemistry*; D006868:Hydrolysis; D018625:Microscopy, Atomic Force; D008970:Molecular Weight; D017433:Protein Structure, Secondary; D017550:Spectroscopy, Fourier Transform Infrared; D000069453:Ultrasonic Waves; D003313:Zea mays / Q000737:chemistry*; D015028:Zein / Q000737:chemistry*
chemical_list: D005983:Glutens; D015028:Zein
keywords: Atomic force microscopy; Corn gluten meal; Dual-frequency power ultrasound; FTIR spectra; Molecular weight distribution; Nano-mechanical properties