title: Sterilization of auto-crosslinked hyaluronic acid scaffolds structured in microparticles and sponges.
abstract: This work evaluated the effects of UV irradiation, plasma radiation, steam and 70% ethanol treatments on the sterilization and integrity of auto-crosslinked hyaluronic acid (HA-ACP) scaffolds structured in microparticles and sponges aiming in vivo applications for regenerative medicine of bone tissue. The integrity of the microparticles was characterized by rheological behavior, while for the sponges, it was characterized by scanning electron microscopy, Fourier transform infrared spectroscopy and differential scanning calorimetry. The effectiveness of the sterilization treatment was verified by the number of microorganism colonies in the samples after the treatments. In conclusion, plasma radiation was the best treatment for the sponges, while steam sterilization in the autoclave at 126°C (1.5 kgf/cm2) for 5 min was the best treatment for the microparticles.
authors.affiliation: Department of Engineering of Materials and Bioprocesses, School of Chemical Engineering, University of Campinas, Campinas-SP, Brazil.
authors.forename: Andréa Arruda Martins
authors.initials: AA
authors.lastname: Shimojo
authors.affiliation: Department of Engineering of Materials and Bioprocesses, School of Chemical Engineering, University of Campinas, Campinas-SP, Brazil.
authors.forename: Isabela Cambraia
authors.initials: IC
authors.lastname: de Souza Brissac
authors.affiliation: Department of Engineering of Materials and Bioprocesses, School of Chemical Engineering, University of Campinas, Campinas-SP, Brazil.
authors.forename: Lucas Martins
authors.initials: LM
authors.lastname: Pina
authors.affiliation: Department of Applied Physics, Institute of Physics "Gleb Wataghin", University of Campinas, Campinas-SP, Brazil.
authors.forename: Carlos Salles
authors.initials: CS
authors.lastname: Lambert
authors.affiliation: Department of Engineering of Materials and Bioprocesses, School of Chemical Engineering, University of Campinas, Campinas-SP, Brazil.
authors.forename: Maria Helena Andrade
authors.initials: MH
authors.lastname: Santana
mesh_terms: D001842:Bone and Bones; D002152:Calorimetry, Differential Scanning; D055598:Chemical Phenomena; D004203:Disinfection / Q000379:methods*; D000431:Ethanol / Q000494:pharmacology; D006820:Hyaluronic Acid / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D053758:Nanoparticles / Q000737:chemistry* / Q000382:microbiology; D011827:Radiation; D017550:Spectroscopy, Fourier Transform Infrared; D013242:Sterilization / Q000379:methods*; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry* / Q000382:microbiology; D014466:Ultraviolet Rays
chemical_list: D000431:Ethanol; D006820:Hyaluronic Acid
keywords: Auto-crosslinked hyaluronic acid; UV irradiation; ethanol treatment; plasma radiation; steam; sterilization