title: A Multi-RNAi Microsponge Platform for Simultaneous Controlled Delivery of Multiple Small Interfering RNAs.
abstract: Packaging multiple small interfering RNA (siRNA) molecules into nanostructures at precisely defined ratios is a powerful delivery strategy for effective RNA interference (RNAi) therapy. We present a novel RNA nanotechnology based approach to produce multiple components of polymerized siRNA molecules that are simultaneously self-assembled and densely packaged into composite sponge-like porous microstructures (Multi-RNAi-MSs) by rolling circle transcription. The Multi-RNAi-MSs were designed to contain a combination of multiple polymeric siRNA molecules with precisely controlled stoichiometry within a singular microstructure by manipulating the types and ratios of the circular DNA templates. The Multi-RNAi-MSs were converted into nanosized complexes by polyelectrolyte condensation to manipulate their physicochemical properties (size, shape, and surface charge) for favorable delivery, while maintaining the multifunctional properties of the siRNAs for combined therapeutic effects. These Multi-RNAi-MS systems have great potential in RNAi-mediated biomedical applications, for example, for the treatment of cancer, genetic disorders, and viral infections.
authors.affiliation: Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.|Koch Institute for Integrative Cancer Research at MIT, Cambridge, MA, 02139, USA.|Department of Biotechnology, Yonsei University, Seoul, 120-749, Republic of Korea.
authors.forename: Young Hoon
authors.initials: YH
authors.lastname: Roh
authors.affiliation: Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.|Koch Institute for Integrative Cancer Research at MIT, Cambridge, MA, 02139, USA.
authors.forename: Jason Z
authors.initials: JZ
authors.lastname: Deng
authors.affiliation: Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.|Koch Institute for Integrative Cancer Research at MIT, Cambridge, MA, 02139, USA.
authors.forename: Erik C
authors.initials: EC
authors.lastname: Dreaden
authors.affiliation: Department of Biotechnology, Yonsei University, Seoul, 120-749, Republic of Korea.
authors.forename: Jae Hyon
authors.initials: JH
authors.lastname: Park
authors.affiliation: Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.|Koch Institute for Integrative Cancer Research at MIT, Cambridge, MA, 02139, USA.
authors.forename: Dong Soo
authors.initials: DS
authors.lastname: Yun
authors.affiliation: Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.|Koch Institute for Integrative Cancer Research at MIT, Cambridge, MA, 02139, USA.
authors.forename: Kevin E
authors.initials: KE
authors.lastname: Shopsowitz
authors.affiliation: Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA. hammond@mit.edu.|Koch Institute for Integrative Cancer Research at MIT, Cambridge, MA, 02139, USA. hammond@mit.edu.
authors.forename: Paula T
authors.initials: PT
authors.lastname: Hammond
mesh_terms: D008855:Microscopy, Electron, Scanning; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000008:administration & dosage*
chemical_list: D034741:RNA, Small Interfering
keywords: DNA/RNA nanotechnology; RNA interference; cancer; multifunctionality; siRNA delivery