title: [Roles of PKCα on the biological functions of T cells].
abstract: OBJECTIVE
  To study the roles of PKCα on the proliferation, apoptosis, differentiation, cytokine production and inducible regulatory T cell (iTreg) induction of T cells.
  
  
  METHODS
  T cells from WT (PKCα⁺/⁺) or PKCα knockout (PKCα⁻/⁻) mice were isolated and cultured in vitro. T cell proliferation and apoptosis were determined using ³H thymidine incorporation and CSFE/Annexin V staining. Cytokines production (IL-2, IL-4, IFN-γ and IL-17) was detected using ELISA. CD4⁺T cells were isolated and cultured in vitro via Th17 or iTreg biased condition. Flow cytometry was used to detect the cell differentiation.
  
  
  RESULTS
  The production of IL-2 upon TCR stimulation increased, while the contents of IL-4 and IL-17 decreased in the PKCα⁻/⁻ group compared with the PKCα⁺/⁺ group. The differentiation rate of Th17 cells decreased, while the iTreg production increased in the PKCα⁻/⁻ group compared with the PKCα⁺/⁺ group.
  
  
  CONCLUSIONS
  PKC-α is proinflammatory.
authors.affiliation: Department of Pediatrics, Xiangya Hosipital, Central South University, Changsha 410008, China. yf2323@hotmail.com.
authors.forename: Li-Fen
authors.initials: LF
authors.lastname: Yang
authors.forename: Hui-Min
authors.initials: HM
authors.lastname: Kong
authors.forename: Xiao-Qing
authors.initials: XQ
authors.lastname: Zhang
authors.forename: Fei
authors.initials: F
authors.lastname: Yin
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D016207:Cytokines / Q000096:biosynthesis; D008213:Lymphocyte Activation; D051379:Mice; D051571:Protein Kinase C-alpha / Q000502:physiology*; D011948:Receptors, Antigen, T-Cell / Q000502:physiology; D013601:T-Lymphocytes / Q000502:physiology*; D058504:Th17 Cells / Q000276:immunology
chemical_list: D016207:Cytokines; D011948:Receptors, Antigen, T-Cell; D051571:Protein Kinase C-alpha