title: Detergent-free Isolation of Functional G Protein-Coupled Receptors into Nanometric Lipid Particles.
abstract: G protein-coupled receptors (GPCRs) are integral membrane proteins that play a pivotal role in signal transduction. Understanding their dynamics is absolutely required to get a clear picture of how signaling proceeds. Molecular characterization of GPCRs isolated in detergents nevertheless stumbles over the deleterious effect of these compounds on receptor function and stability. We explored here the potential of a styrene-maleic acid polymer to solubilize receptors directly from their lipid environment. To this end, we used two GPCRs, the melatonin and ghrelin receptors, embedded in two membrane systems of increasing complexity, liposomes and membranes from Pichia pastoris. The styrene-maleic acid polymer was able, in both cases, to extract membrane patches of a well-defined size. GPCRs in SMA-stabilized lipid discs not only recognized their ligand but also transmitted a signal, as evidenced by their ability to activate their cognate G proteins and recruit arrestins in an agonist-dependent manner. Besides, the purified receptor in lipid discs undergoes all specific changes in conformation associated with ligand-mediated activation, as demonstrated in the case of the ghrelin receptor with fluorescent conformational reporters and compounds from distinct pharmacological classes. Altogether, these data highlight the potential of styrene-maleic stabilized lipid discs for analyzing the molecular bases of GPCR-mediated signaling in a well-controlled membrane-like environment.
authors.affiliation: Pole d'expertise Biotechnologie, Chimie, Biologie, Institut de Recherches Servier , 125, chemin de Ronde, F-78290 Croissy-sur-Seine, France.
authors.forename: Christel
authors.initials: C
authors.lastname: Logez
authors.affiliation: Faculté de Pharmacie, Institut des Biomolécules Max Mousseron (IBMM), UMR 5247 CNRS-Université Montpellier-ENSCM , 15 Avenue C. Flahault, F-34093 Montpellier, France.
authors.forename: Marjorie
authors.initials: M
authors.lastname: Damian
authors.affiliation: Pole d'expertise Biotechnologie, Chimie, Biologie, Institut de Recherches Servier , 125, chemin de Ronde, F-78290 Croissy-sur-Seine, France.
authors.forename: Céline
authors.initials: C
authors.lastname: Legros
authors.affiliation: Pole d'expertise Biotechnologie, Chimie, Biologie, Institut de Recherches Servier , 125, chemin de Ronde, F-78290 Croissy-sur-Seine, France.
authors.forename: Clémence
authors.initials: C
authors.lastname: Dupré
authors.affiliation: Faculté de Pharmacie, Institut des Biomolécules Max Mousseron (IBMM), UMR 5247 CNRS-Université Montpellier-ENSCM , 15 Avenue C. Flahault, F-34093 Montpellier, France.
authors.forename: Mélody
authors.initials: M
authors.lastname: Guéry
authors.affiliation: Faculté de Pharmacie, Institut des Biomolécules Max Mousseron (IBMM), UMR 5247 CNRS-Université Montpellier-ENSCM , 15 Avenue C. Flahault, F-34093 Montpellier, France.
authors.forename: Sophie
authors.initials: S
authors.lastname: Mary
authors.affiliation: CNRS UMR7242, Institut de Recherche de l'ESBS, Biotechnologie et Signalisation Cellulaire, Université de Strasbourg , 300 Boulevard Sébastien Brant, 67412 Ilkirch cedex, France.
authors.forename: Renaud
authors.initials: R
authors.lastname: Wagner
authors.affiliation: Faculté de Pharmacie, Institut des Biomolécules Max Mousseron (IBMM), UMR 5247 CNRS-Université Montpellier-ENSCM , 15 Avenue C. Flahault, F-34093 Montpellier, France.
authors.forename: Céline
authors.initials: C
authors.lastname: M'Kadmi
authors.affiliation: Pole d'expertise Biotechnologie, Chimie, Biologie, Institut de Recherches Servier , 125, chemin de Ronde, F-78290 Croissy-sur-Seine, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Nosjean
authors.affiliation: Pole d'expertise Biotechnologie, Chimie, Biologie, Institut de Recherches Servier , 125, chemin de Ronde, F-78290 Croissy-sur-Seine, France.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Fould
authors.affiliation: Faculté de Pharmacie, Institut des Biomolécules Max Mousseron (IBMM), UMR 5247 CNRS-Université Montpellier-ENSCM , 15 Avenue C. Flahault, F-34093 Montpellier, France.
authors.forename: Jacky
authors.initials: J
authors.lastname: Marie
authors.affiliation: Faculté de Pharmacie, Institut des Biomolécules Max Mousseron (IBMM), UMR 5247 CNRS-Université Montpellier-ENSCM , 15 Avenue C. Flahault, F-34093 Montpellier, France.
authors.forename: Jean-Alain
authors.initials: JA
authors.lastname: Fehrentz
authors.affiliation: Faculté de Pharmacie, Institut des Biomolécules Max Mousseron (IBMM), UMR 5247 CNRS-Université Montpellier-ENSCM , 15 Avenue C. Flahault, F-34093 Montpellier, France.
authors.forename: Jean
authors.initials: J
authors.lastname: Martinez
authors.affiliation: Pole d'expertise Biotechnologie, Chimie, Biologie, Institut de Recherches Servier , 125, chemin de Ronde, F-78290 Croissy-sur-Seine, France.
authors.forename: Gilles
authors.initials: G
authors.lastname: Ferry
authors.affiliation: Pole d'expertise Biotechnologie, Chimie, Biologie, Institut de Recherches Servier , 125, chemin de Ronde, F-78290 Croissy-sur-Seine, France.
authors.forename: Jean A
authors.initials: JA
authors.lastname: Boutin
authors.affiliation: Faculté de Pharmacie, Institut des Biomolécules Max Mousseron (IBMM), UMR 5247 CNRS-Université Montpellier-ENSCM , 15 Avenue C. Flahault, F-34093 Montpellier, France.
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Banères
mesh_terms: D000818:Animals; D016466:CHO Cells; D003412:Cricetulus; D019204:GTP-Binding Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D006801:Humans; D008055:Lipids / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry*; D008298:Maleates / Q000737:chemistry*; D008958:Models, Molecular; D049329:Nanostructures / Q000737:chemistry*; D010843:Pichia / Q000737:chemistry / Q000378:metabolism; D011137:Polystyrenes / Q000737:chemistry*; D054440:Receptors, Ghrelin / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D044094:Receptors, Melatonin / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D012995:Solubility
chemical_list: D008055:Lipids; D008081:Liposomes; D008298:Maleates; D011137:Polystyrenes; D054440:Receptors, Ghrelin; D044094:Receptors, Melatonin; C024074:styrene-maleic acid polymer; D019204:GTP-Binding Proteins