title: Oral Bifidobacterium longum expressing alpha-melanocyte-stimulating hormone to fight experimental colitis.
abstract: The oral delivery of peptides is a highly attractive treatment approach. However, the harsh environment of the gastrointestinal tract limits its application. Here, we utilize Bifidobacterium as a delivery system to orally deliver a potent anti-inflammatory but short duration peptide alpha-melanocyte-stimulating hormone (α-MSH) against experimental colitis. The aim of our study was to facilitate the efficient oral delivery of α-MSH. We designed a vector of pBDMSH and used it to construct a Bifidobacterium longum expressing α-MSH. We then determined the bioactivity of recombinant Bifidobacterium in lipopolysaccharide-induced inflammatory models of HT-29 cells. Finally, we used Bifidobacterium expressing α-MSH against dextran sulfate sodium (DSS)-induced ulcerative colitis mice. Results based on the myeloperoxidase activity, the levels of inflammatory cytokines TNF-α, IL-1β, IL-6, and IL-10 and the histological injury of colon tissue reveal recombinant Bifidobacterium was efficient in attenuating DSS-induced ulcerative colitis, suggesting an alternative way to use Bifidobacterium as a delivery system to deliver α-MSH for DSS-induced ulcerative colitis therapy.
authors.affiliation: a Institute of Genomic Medicine Research, College of Pharmacy, Jinan University , Guangzhou , People's Republic of China.
authors.forename: Pijin
authors.initials: P
authors.lastname: Wei
authors.affiliation: a Institute of Genomic Medicine Research, College of Pharmacy, Jinan University , Guangzhou , People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Yang
authors.affiliation: a Institute of Genomic Medicine Research, College of Pharmacy, Jinan University , Guangzhou , People's Republic of China.
authors.forename: Zhaobing
authors.initials: Z
authors.lastname: Liu
authors.affiliation: a Institute of Genomic Medicine Research, College of Pharmacy, Jinan University , Guangzhou , People's Republic of China.
authors.forename: Junli
authors.initials: J
authors.lastname: Huang
authors.affiliation: a Institute of Genomic Medicine Research, College of Pharmacy, Jinan University , Guangzhou , People's Republic of China.
authors.forename: Yahui
authors.initials: Y
authors.lastname: Gong
authors.affiliation: a Institute of Genomic Medicine Research, College of Pharmacy, Jinan University , Guangzhou , People's Republic of China.
authors.forename: Hanxiao
authors.initials: H
authors.lastname: Sun
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D000069978:Bifidobacterium longum; D003106:Colon / Q000737:chemistry* / Q000187:drug effects; D016264:Dextran Sulfate / Q000737:chemistry*; D019073:HT29 Cells; D006801:Humans; D016753:Interleukin-10 / Q000737:chemistry* / Q000494:pharmacology; D051379:Mice; D014409:Tumor Necrosis Factor-alpha / Q000737:chemistry*; D000521:alpha-MSH / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D000893:Anti-Inflammatory Agents; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D000521:alpha-MSH; D016264:Dextran Sulfate
keywords: Bifidobacterium; colitis; peptide delivery; α-MSH