title: DNA barcoding Indian freshwater fishes.
abstract: DNA barcoding is a promising technique for species identification using a short mitochondrial DNA sequence of cytochrome c oxidase I (COI) gene. In the present study, DNA barcodes were generated from 72 species of freshwater fish covering the Orders Cypriniformes, Siluriformes, Perciformes, Synbranchiformes, and Osteoglossiformes representing 50 genera and 19 families. All the samples were collected from diverse sites except the species endemic to a particular location. Species were represented by multiple specimens in the great majority of the barcoded species. A total of 284 COI sequences were generated. After amplification and sequencing of 700 base pair fragment of COI, primers were trimmed which invariably generated a 655 base pair barcode sequence. The average Kimura two-parameter (K2P) distances within-species, genera, families, and orders were 0.40%, 9.60%, 13.10%, and 17.16%, respectively. DNA barcode discriminated congeneric species without any confusion. The study strongly validated the efficiency of COI as an ideal marker for DNA barcoding of Indian freshwater fishes.
authors.affiliation: a Central Institute of Fisheries Education , Versova , Mumbai , India.
authors.forename: Wazir Singh
authors.initials: WS
authors.lastname: Lakra
authors.affiliation: b National Bureau of Fish Genetic Resources , Lucknow , India.
authors.forename: M
authors.initials: M
authors.lastname: Singh
authors.affiliation: a Central Institute of Fisheries Education , Versova , Mumbai , India.
authors.forename: Mukunda
authors.initials: M
authors.lastname: Goswami
authors.affiliation: b National Bureau of Fish Genetic Resources , Lucknow , India.
authors.forename: A
authors.initials: A
authors.lastname: Gopalakrishnan
authors.affiliation: b National Bureau of Fish Genetic Resources , Lucknow , India.
authors.forename: K K
authors.initials: KK
authors.lastname: Lal
authors.affiliation: b National Bureau of Fish Genetic Resources , Lucknow , India.
authors.forename: V
authors.initials: V
authors.lastname: Mohindra
authors.affiliation: b National Bureau of Fish Genetic Resources , Lucknow , India.
authors.forename: U K
authors.initials: UK
authors.lastname: Sarkar
authors.affiliation: b National Bureau of Fish Genetic Resources , Lucknow , India.
authors.forename: P P
authors.initials: PP
authors.lastname: Punia
authors.affiliation: b National Bureau of Fish Genetic Resources , Lucknow , India.
authors.forename: K V
authors.initials: KV
authors.lastname: Singh
authors.affiliation: c Department of Zoology and Biotechnology , HNB Garhwal University , Uttarakhand , India , and.
authors.forename: J P
authors.initials: JP
authors.lastname: Bhatt
authors.affiliation: d Indian Council of Agricultural Research , New Delhi , India.
authors.forename: S
authors.initials: S
authors.lastname: Ayyappan
mesh_terms: D000818:Animals; D002397:Catfishes / Q000145:classification / Q000235:genetics; D003531:Cypriniformes / Q000145:classification / Q000235:genetics; D058893:DNA Barcoding, Taxonomic; D004272:DNA, Mitochondrial / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D030541:Databases, Genetic; D003576:Electron Transport Complex IV / Q000145:classification / Q000235:genetics / Q000378:metabolism; D005399:Fishes / Q000145:classification / Q000235:genetics*; D005618:Fresh Water; D014644:Genetic Variation; D010473:Perciformes / Q000145:classification / Q000235:genetics; D010802:Phylogeny
chemical_list: D004272:DNA, Mitochondrial; D003576:Electron Transport Complex IV
keywords: Barcoding; COI; Indian freshwater fishes; mtDNA