title: Biorepository for Pediatric Cancer with Minimal Resources: Meeting the Challenges.
abstract: BACKGROUND
  Conducting high throughput -omics research requires high quality, data-rich biospecimens to unravel factors underlying childhood cancers; this is an extra burden in a limited resources country. For this purpose, Children's Cancer Hospital (CCHE), the largest pediatric cancer hospital worldwide, established a cutting-edge Biorepository and Biospecimen Research Facility (CCHE-BBR).
  
  
  OBJECTIVE
  To present a step-by-step guide to establishing a hospital-based biorepository with limited resources, and working in collaboration with different hospital facilities to supply the research community with high quality data-rich biospecimens fit for a wide range of research purposes. This approach will foster research in the era of personalized precision medicine.
  
  
  METHODS
  CCHE-IRB approved the collection and storage of biospecimens from patients and parents for future research. We focused on staff training, recruiting qualified scientists, and establishing the infrastructure. The CCHE Biorepository developed strict standardized procedures for sample acquisition, processing, annotation, storage, and distribution based on ISBER Best Practices and CAP-accreditation guidelines. We collect samples at different clinical time points (e.g., at remission and/or relapse) as well as parents' samples for genetic studies. Using CaTissue®, an electronic storage management system, allowed sample annotation and full integration with clinical data and the cancer registry.
  
  
  RESULTS
  In 2 years, we succeeded in establishing a well-designed biorepository within our regulations, bylaws, and SOPs, and with a minimal budget. We store high quality blood derivatives, CSF, and malignant/normal tissue samples.
  
  
  CONCLUSIONS
  Building a high quality biorepository with minimal-resources to encourage research is possible. Having the suitable infrastructure with a significant number of clinically annotated samples can play a major role in international research projects, sharing samples and/or data with other groups.
authors.affiliation: 1 Biorepository and Biospecimen Research, Children's Cancer Hospital , Cairo, Egypt .
authors.forename: Rania M
authors.initials: RM
authors.lastname: Labib
authors.affiliation: 1 Biorepository and Biospecimen Research, Children's Cancer Hospital , Cairo, Egypt .
authors.forename: Mahmoud M
authors.initials: MM
authors.lastname: Mostafa
authors.affiliation: 1 Biorepository and Biospecimen Research, Children's Cancer Hospital , Cairo, Egypt .
authors.forename: Ahmad S
authors.initials: AS
authors.lastname: Alfaar
authors.affiliation: 2 Medical Engineering Department, Children's Cancer Hospital , Cairo, Egypt .
authors.forename: Doaa
authors.initials: D
authors.lastname: Yehia
authors.affiliation: 1 Biorepository and Biospecimen Research, Children's Cancer Hospital , Cairo, Egypt .
authors.forename: Maram
authors.initials: M
authors.lastname: Alaa
authors.affiliation: 1 Biorepository and Biospecimen Research, Children's Cancer Hospital , Cairo, Egypt .
authors.forename: Mariam G
authors.initials: MG
authors.lastname: Elzayat
authors.affiliation: 1 Biorepository and Biospecimen Research, Children's Cancer Hospital , Cairo, Egypt .
authors.forename: Mohamed
authors.initials: M
authors.lastname: Adel
authors.affiliation: 1 Biorepository and Biospecimen Research, Children's Cancer Hospital , Cairo, Egypt .
authors.forename: Sameera
authors.initials: S
authors.lastname: Ezzat
authors.affiliation: 1 Biorepository and Biospecimen Research, Children's Cancer Hospital , Cairo, Egypt .
authors.forename: Sherif
authors.initials: S
authors.lastname: Abo El-Naga
mesh_terms: D035843:Biomedical Research; D057285:Precision Medicine / Q000379:methods; D013048:Specimen Handling / Q000379:methods; D014015:Tissue Banks