title: RAPD and ISSR based evaluation of genetic stability of micropropagated plantlets of Morus alba L. variety S-1.
abstract: Plant regeneration through rapid in vitro clonal propagation of nodal explants of Morus alba L. variety S-1 was established along with genetic stability analysis of regenerates. Axillary shoot bud proliferation was achieved on Murashige and Skoog (MS) medium in various culture regimes. Highest number of shoots (5.62 ± 0.01), with average length 4.19 ± 0.01 cm, was initially achieved with medium containing 0.5 mg/l N(6)-benzyladenine (BA) and 3% sucrose. Repeated subculturing of newly formed nodal parts after each harvest up to sixth passage, yielded highest number of shoots (about 32.27) per explants was obtained after fourth passage. Rooting of shoots occurred on 1/2 MS medium supplemented with 1.0 mg/1 Indole-3-butyric acid (IBA). About 90% (89.16) of the plantlets transferred to the mixture of sand:soil:organic manure (2:2:1) in small plastic pots acclimatized successfully. Genetic stability of the discussed protocol was confirmed by two DNA-based fingerprinting techniques i.e. RAPD (random amplified polymorphic DNA) and ISSR (inter-simple sequence repeat). This protocol can be used for commercial propagation and for future genetic improvement studies.
authors.affiliation: Cytogenetics & Plant Biotechnology Research Unit, Department of Botany, University of Kalyani, Kalyani, 741235 Nadia, West Bengal, India.
authors.forename: Soumen
authors.initials: S
authors.lastname: Saha
authors.affiliation: Cytogenetics & Plant Biotechnology Research Unit, Department of Botany, University of Kalyani, Kalyani, 741235 Nadia, West Bengal, India.
authors.forename: Sinchan
authors.initials: S
authors.lastname: Adhikari
authors.affiliation: Department of Botany, Kalyani Mahavidyalaya, City Centre Complex, Kalyani, Nadia 741235, West Bengal, India.
authors.forename: Tulsi
authors.initials: T
authors.lastname: Dey
authors.affiliation: Cytogenetics & Plant Biotechnology Research Unit, Department of Botany, University of Kalyani, Kalyani, 741235 Nadia, West Bengal, India.
authors.forename: Parthadeb
authors.initials: P
authors.lastname: Ghosh
keywords: DNA-based markers; In vitro; Micropropagation; Morus alba L. variety S-1; Nodal explants