title: Improvement of green tea polyphenol with milk on skin with respect to antioxidation in healthy adults: a double-blind placebo-controlled randomized crossover clinical trial.
abstract: Green tea polyphenols (GTP) have been widely tested for their effects on several metabolic syndromes and degenerative diseases such as cancer, cardiovascular diseases, and diabetes. The present study was formulated to assess the physiological efficacy of green tea polyphenol infused with milk (GTPM) on skin integrity in correlation with antioxidative status in healthy adults. Forty-four healthy voluntary subjects were recruited and assigned to two groups, who drank 240 ml of mineral water mixed with either an experimental (GTPM) or placebo package (2 packs per day) for the following 6 months. The experimental group then switched to the placebo package, and vice versa, for a further 6 months, with one month of washout period in between. During the initial, 3(rd), 6(th), 10(th), and 13(th) month anthropometric measurements were performed and fasting blood samples were withdrawn for various biochemical assays. Skin examination was performed at the initial, 6(th) and 13(th) month. No significant alterations were observed in any of the anthropometric measurements. Administration of GTPM significantly increased (p < 0.05) the antioxidant index and antioxidant enzyme activities when compared with the placebo group, whereas a concomitant decrease in the levels of lipid peroxidation were noted. Moreover, GTPM intake notably improved skin integrity and texture by markedly lowering (p < 0.05) skin wrinkles and roughness in elderly subjects. GTPM proved to be an effective antioxidant by lowering oxidative stress and thereby ameliorating skin texture and integrity.
authors.affiliation: Department of Chinese Medicine, Taichung Hospital Ministry of Health and Well-being, Taichung, Taiwan, Republic of China.
authors.forename: Hui-Fang
authors.initials: HF
authors.lastname: Chiu
authors.affiliation: School of Nutrition, Chung Shan Medical University, 110, Sec. 1, Jianguo North Road, Taichung City, Taiwan, Republic of China.
authors.forename: Tung-Yi
authors.initials: TY
authors.lastname: Lin
authors.affiliation: School of Nutrition, Chung Shan Medical University, 110, Sec. 1, Jianguo North Road, Taichung City, Taiwan, Republic of China. wck@csmu.edu.tw.
authors.forename: You-Cheng
authors.initials: YC
authors.lastname: Shen
authors.affiliation: School of Nutrition, Chung Shan Medical University, 110, Sec. 1, Jianguo North Road, Taichung City, Taiwan, Republic of China.
authors.forename: Kamesh
authors.initials: K
authors.lastname: Venkatakrishnan
authors.affiliation: School of Nutrition, Chung Shan Medical University, 110, Sec. 1, Jianguo North Road, Taichung City, Taiwan, Republic of China.
authors.forename: Chin-Kun
authors.initials: CK
authors.lastname: Wang
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D000975:Antioxidants / Q000032:analysis / Q000494:pharmacology*; D018592:Cross-Over Studies; D004311:Double-Blind Method; D005260:Female; D005419:Flavonoids / Q000032:analysis / Q000494:pharmacology; D005707:Gallic Acid / Q000032:analysis / Q000494:pharmacology; D006801:Humans; D062385:Hydroxybenzoates / Q000032:analysis / Q000494:pharmacology; D008297:Male; D008875:Middle Aged; D008892:Milk / Q000737:chemistry*; D018384:Oxidative Stress / Q000187:drug effects; D059808:Polyphenols / Q000032:analysis / Q000494:pharmacology*; D012867:Skin / Q000187:drug effects*; D013634:Tannins / Q000032:analysis / Q000494:pharmacology; D013662:Tea / Q000737:chemistry*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D062385:Hydroxybenzoates; D059808:Polyphenols; D013634:Tannins; D013662:Tea; D017392:Thiobarbituric Acid Reactive Substances; D005707:Gallic Acid; C017616:phenolic acid