title: Mass Determination of Entire Amyloid Fibrils by Using Mass Spectrometry.
abstract: Amyloid fibrils are self-assembled protein structures with important roles in biology (either pathogenic or physiological), and are attracting increasing interest in nanotechnology. However, because of their high aspect ratio and the presence of some polymorphism, that is, the possibility to adopt various structures, their characterization is challenging and basic information such as their mass is unknown. Here we show that charge-detection mass spectrometry, recently developed for large self-assembled systems such as viruses, provides such information in a straightforward manner.
authors.affiliation: Institut Lumière Matière, UMR5306 Université Lyon 1-CNRS, Université de Lyon, 5 rue de la Doua, 69622, Villeurbanne, France.
authors.forename: Tristan
authors.initials: T
authors.lastname: Doussineau
authors.affiliation: CEA, DSV, iRTSV, Laboratoire de Chimie et Biologie des Métaux, UMR5249 CEA-CNRS-UJF, CEA Grenoble, 17 rue des Martyrs, 38054, Grenoble, France.
authors.forename: Carole
authors.initials: C
authors.lastname: Mathevon
authors.affiliation: CEA, DSV, iRTSV, Laboratoire de Chimie et Biologie des Métaux, UMR5249 CEA-CNRS-UJF, CEA Grenoble, 17 rue des Martyrs, 38054, Grenoble, France.
authors.forename: Lucie
authors.initials: L
authors.lastname: Altamura
authors.affiliation: ERRMECe, I-MAT FD4122, Université de Cergy-Pontoise, France.|LMGP, CNRS UMR 5628, Grenoble, France.
authors.forename: Charlotte
authors.initials: C
authors.lastname: Vendrely
authors.affiliation: Institut Lumière Matière, UMR5306 Université Lyon 1-CNRS, Université de Lyon, 5 rue de la Doua, 69622, Villeurbanne, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Dugourd
authors.affiliation: CEA, DSV, iRTSV, Laboratoire de Chimie et Biologie des Métaux, UMR5249 CEA-CNRS-UJF, CEA Grenoble, 17 rue des Martyrs, 38054, Grenoble, France. vincent.forge@cea.fr.
authors.forename: Vincent
authors.initials: V
authors.lastname: Forge
authors.affiliation: Institut Lumière Matière, UMR5306 Université Lyon 1-CNRS, Université de Lyon, 5 rue de la Doua, 69622, Villeurbanne, France. rodolphe.antoine@univ-lyon1.fr.
authors.forename: Rodolphe
authors.initials: R
authors.lastname: Antoine
mesh_terms: D000682:Amyloid / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods*; D046529:Microscopy, Electron, Transmission; D008970:Molecular Weight
chemical_list: D000682:Amyloid
keywords: amyloids; fibrils; mass spectrometry; proteins; self-assembly