title: Selective Detection of 8-Oxo-2'-deoxyguanosine in Single-Stranded DNA via Nanopore Sensing Approach.
abstract: We have developed a nanopore sensing approach for the selective detection of 8-oxo-2'-deoxyguanosine (8-oxoG) in single-stranded DNA. First, 1,12-dodecanediamine is coupled with 8-oxoG-containing DNA molecules in high yield which leaves a free amine group for subsequent attaching of an adamantane moiety. After incubation with cucurbit[7]uril, the host-guest complex-modified DNA hybrid is translocated through an α-hemolysin nanopore. Highly characteristic events can be recorded and used to quantify the 8-oxoG-DNA content in a DNA mixture. Compared with the existing methods, this study provides a reliable, quick, and low-cost approach for the detection of 8-oxoG site in single-stranded DNA at the single-molecule level, particularly suitable for high-throughput screening of a massive number of samples.
authors.affiliation: Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety, Institute of High Energy Physics, Chinese Academy of Sciences , Beijing 100049, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.affiliation: Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety, Institute of High Energy Physics, Chinese Academy of Sciences , Beijing 100049, China.
authors.forename: Yuru
authors.initials: Y
authors.lastname: Li
authors.affiliation: Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety, Institute of High Energy Physics, Chinese Academy of Sciences , Beijing 100049, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Li
authors.affiliation: Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety, Institute of High Energy Physics, Chinese Academy of Sciences , Beijing 100049, China.
authors.forename: Jiani
authors.initials: J
authors.lastname: Xie
authors.affiliation: Multidisciplinary Center, Institute of High Energy Physics, Chinese Academy of Sciences , Beijing 100049, China.
authors.forename: Chaofei
authors.initials: C
authors.lastname: Chen
authors.affiliation: Multidisciplinary Center, Institute of High Energy Physics, Chinese Academy of Sciences , Beijing 100049, China.
authors.forename: Quansheng
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Epilepsy Department, Beijing ChaoYang Emergency Medical Center , Beijing 100021, China.
authors.forename: Shouwen
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety, Institute of High Energy Physics, Chinese Academy of Sciences , Beijing 100049, China.
authors.forename: Hai-Chen
authors.initials: HC
authors.lastname: Wu
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D004277:DNA, Single-Stranded / Q000737:chemistry*; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000032:analysis; D008958:Models, Molecular; D015394:Molecular Structure; D058608:Nanopores
chemical_list: D004277:DNA, Single-Stranded; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine