title: Salicylic acid-based poly(anhydride-ester) nerve guidance conduits: Impact of localized drug release on nerve regeneration.
abstract: Nerve guidance conduits (NGCs) can serve as physical scaffolds aligning and supporting regenerating cells while preventing scar tissue formation that often interferes with the regeneration process. Numerous studies have focused on functionalizing NGCs with neurotrophic factors, for example, to support nerve regeneration over longer gaps, but few directly incorporate therapeutic agents. Herein, we fabricated NGCs from a polyanhydride comprised of salicylic acid (SA), a nonsteroidal anti-inflammatory drug, then performed in vitro and in vivo assays. In vitro studies included cytotoxicity, anti-inflammatory response, and NGC porosity measurements. To prepare for implantation, type I collagen hydrogels were used as NGC luminal fillers to further enhance the axonal regeneration process. For the in vivo studies, SA-NGCs were implanted in femoral nerves of mice for 16 weeks and evaluated for functional recovery. The SA-based NGCs functioned as both a drug delivery vehicle capable of reducing inflammation and scar tissue formation because of SA release as well as a tissue scaffold that promotes peripheral nerve regeneration and functional recovery.
authors.affiliation: Department of Biomedical Engineering, Rutgers University, Piscataway, New Jersey 08854.
authors.forename: Yong S
authors.initials: YS
authors.lastname: Lee
authors.affiliation: Department of Biomedical Engineering, Rutgers University, Piscataway, New Jersey 08854.
authors.forename: Jeremy
authors.initials: J
authors.lastname: Griffin
authors.affiliation: Department of Biomedical Engineering, Rutgers University, Piscataway, New Jersey 08854.
authors.forename: Shirley N
authors.initials: SN
authors.lastname: Masand
authors.affiliation: Department of Biomedical Engineering, Rutgers University, Piscataway, New Jersey 08854.
authors.forename: David I
authors.initials: DI
authors.lastname: Shreiber
authors.affiliation: Department of Biomedical Engineering, Rutgers University, Piscataway, New Jersey 08854.|Department of Chemistry and Chemical Biology, Rutgers University, Piscataway, New Jersey, 08854.
authors.forename: Kathryn E
authors.initials: KE
authors.lastname: Uhrich
mesh_terms: D000812:Anhydrides / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology; D002460:Cell Line; D045744:Cell Line, Tumor; D005260:Female; D005269:Femur / Q000294:innervation*; D048091:Guided Tissue Regeneration / Q000379:methods*; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D009416:Nerve Regeneration / Q000187:drug effects*; D010525:Peripheral Nerves / Q000187:drug effects / Q000502:physiology*; D016062:Porosity; D020156:Salicylic Acid / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D000812:Anhydrides; D000893:Anti-Inflammatory Agents; D020156:Salicylic Acid
keywords: drug delivery; nerve regeneration; never guidance conduit; salicylic acid; tissue engineering