title: Self-Assembled Wound Dressings Silence MMP-9 and Improve Diabetic Wound Healing In Vivo.
abstract: The direct local delivery of short interfering RNA (siRNA) into target tissues presents a real solution to several complex medical conditions that today lack efficacious therapies. The development of an ultrathin polymer coating is described to sustain the delivery of siRNA for up to 2 weeks in vitro and in vivo. This technology successfully reduces the expression of MMP-9 within the wounds of diabetic mice, significantly accelerating the wound healing process and improving the quality of tissue formed.
authors.affiliation: Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.|Koch Institute of Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.|Institute for Soldier Nanotechnologies, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.|Harvard-MIT Division of Health Sciences and Technology, Cambridge, MA, 02139, USA.
authors.forename: Steven A
authors.initials: SA
authors.lastname: Castleberry
authors.affiliation: Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.|Koch Institute of Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.|Institute for Soldier Nanotechnologies, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.
authors.forename: Benjamin D
authors.initials: BD
authors.lastname: Almquist
authors.affiliation: Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.|Koch Institute of Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.
authors.forename: Tiago
authors.initials: T
authors.lastname: Reis
authors.affiliation: Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.
authors.forename: John
authors.initials: J
authors.lastname: Chow
authors.affiliation: Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.
authors.forename: Sarah
authors.initials: S
authors.lastname: Mayner
authors.affiliation: Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.|Koch Institute of Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.|Institute for Soldier Nanotechnologies, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.
authors.forename: Paula T
authors.initials: PT
authors.lastname: Hammond
mesh_terms: D000818:Animals; D001458:Bandages; D003921:Diabetes Mellitus, Experimental / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology*; D055785:Gene Knockdown Techniques; D020868:Gene Silencing; D020780:Matrix Metalloproteinase 9 / Q000172:deficiency* / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D041681:NIH 3T3 Cells; D059748:Proteolysis; D034741:RNA, Small Interfering / Q000235:genetics*; D014945:Wound Healing / Q000235:genetics*
chemical_list: D034741:RNA, Small Interfering; D020780:Matrix Metalloproteinase 9
keywords: controlled local delivery; diabetic wound healing; layer-by-layer; polymer coatings; siRNA delivery