title: Establishment of an Agrobacterium-mediated Inoculation System for Cucumber Green Mottle Mosaic Virus.
abstract: The infectious full-length cDNA clones of Cucumber green mottle mosaic virus (CGMMV) isolates KW and KOM, which were isolated from watermelon and oriental melon, respectively, were constructed under the control of the cauliflower mosaic virus 35S promoter. We successfully inoculated Nicotiana benthamiana with the cloned CGMMV isolates KW and KOM by Agrobacterium-mediated infiltration. Virulence and symptomatic characteristics of the cloned CGMMV isolates KW and KOM were tested on several indicator plants. No obvious differences between two cloned isolates in disease development were observed on the tested indicator plants. We also determined full genome sequences of the cloned CGMMV isolates KW and KOM. Sequence comparison revealed that only four amino acids (at positions 228, 699, 1212, and 1238 of the replicase protein region) differ between the cloned isolates KW and KOM. A previous study reported that the isolate KOM could not infect Chenopodium amaranticolor, but the cloned KOM induced chlorotic spots on the inoculated leaves. When compared with the previously reported sequence of the original KOM isolate, the cloned KOM contained one amino acid mutation (Ala to Thr) at position 228 of the replicase protein, suggesting that this mutation might be responsible for induction of chlorotic spots on the inoculated leaves of C. amaranticolor.
authors.affiliation: Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Korea.
authors.forename: Minji
authors.initials: M
authors.lastname: Kang
authors.affiliation: Crop Protection Division, National Academy of Agricultural Science, Wanju 565-852, Korea.
authors.forename: Jang-Kyun
authors.initials: JK
authors.lastname: Seo
authors.affiliation: Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Korea.
authors.forename: Dami
authors.initials: D
authors.lastname: Song
authors.affiliation: Crop Protection Division, National Academy of Agricultural Science, Wanju 565-852, Korea.
authors.forename: Hong-Soo
authors.initials: HS
authors.lastname: Choi
authors.affiliation: Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Korea ; Plant Genomics and Breeding Institute, Seoul National University, Seoul 151-921, Korea.
authors.forename: Kook-Hyung
authors.initials: KH
authors.lastname: Kim
keywords: CGMMV; agroinfiltration; infectious clone