title: Elevated homocysteine levels in suction-induced blister fluid of active vitiligo lesions.
abstract: BACKGROUND
  Vitiligo is the most prevalent acquired pigmentary disorder as a result of destruction of melanocytes. Several studies have reported increased serum levels of homocysteine (Hcy) in vitiligo patients which may be the result of decreased Vitamin B12 and folic acid levels. In addition, homocystinuria is associated with pigmentary dilution. On the other hand, other studies reported normal serum homocysteine levels.
  
  
  OBJECTIVE
  Our aim was to study the Hcy level in active vitiligo patients both in serum and in suction blister fluid obtained from the lesional skin.
  
  
  METHODS
  A total of 30 patients with active vitiligo of both sexes and 30 healthy volunteers were enrolled in this study. Sera from the blood and from lesional induced bullae were obtained from the patients and controls and were assayed for Hcy by enzyme-linked immunosorbent assay (ELISA). The collected data were analyzed by SPSS version 17.
  
  
  RESULTS
  There were no significant differences in the serum levels of Hcy between patients and healthy controls, however, the increase in Hcy level was highly statistically significant in the patients' lesional induced bulla compared to the healthy controls. There was no significant difference in Hcy levels between males and females and between patients with negative or positive family histories of vitiligo.
  
  
  CONCLUSIONS
  The presence of a high homocysteine level in active vitiligo lesions points to a local event occurring in this lesion, which is not reflected as an increase in the patient's serum level.
authors.affiliation: Minia University, Minia, Egypt.
authors.forename: Tag
authors.initials: T
authors.lastname: Anbar
authors.affiliation: Ain-Shams University, Cairo, Egypt, Dermatology, Venereology and Andrology Department, Ain-Shams University Hospitals, Ramses Street, Abbassia, Cairo 11566, Egypt.
authors.forename: Nehal Mohamed
authors.initials: NM
authors.lastname: Zuel-Fakkar
authors.affiliation: Ain-Shams University, Cairo, Egypt, Dermatology, Venereology and Andrology Department, Ain-Shams University Hospitals, Ramses Street, Abbassia, Cairo 11566, Egypt.
authors.forename: Mary Fikry
authors.initials: MF
authors.lastname: Matta
authors.affiliation: Juba University, Sudan.
authors.forename: Mai Mohammed Ibrahim
authors.initials: MM
authors.lastname: Arbab
mesh_terms: D000328:Adult; D001768:Blister / Q000378:metabolism*; D005260:Female; D006710:Homocysteine / Q000378:metabolism*; D006801:Humans; D008297:Male; D013396:Suction; D014820:Vitiligo / Q000097:blood / Q000378:metabolism*
chemical_list: D006710:Homocysteine
keywords: homocysteine; vitiligo