title: Nonexpanded Adipose Stromal Vascular Fraction Local Therapy on Peripheral Nerve Regeneration Using Allografts.
abstract: OBJECTIVE
  Adipose tissue possesses a population of multi-potent stem cells which can be differentiated to a Schwann cell phenotype and may be of benefit for treatment of peripheral nerve injuries. Effects of local therapy of nonexpanded adipose stromal vascular fraction (SVF) on peripheral nerve regeneration was studied using allografts in a rat sciatic nerve model.
  
  
  METHODS
  Thirty male white Wistar rats were divided into three experimental groups (n = 10), randomly: Sham-operated group (SHAM), allograft group (ALLO), SVF-treated group (ALLO/SVF). In SHAM group left sciatic nerve was exposed through a gluteal muscle incision and after homeostasis muscle was sutured. In the ALLO group the left sciatic nerve was exposed through a gluteal muscle incision and transected proximal to the tibio-peroneal bifurcation where a 10 mm segment was excised. The same procedure was performed in the ALLO/SVF group. The harvested nerves of the rats of ALLO group were served as allograft for ALLO/SVF group and vice versa. The SHAM and ALLO groups received 100 μL phosphate buffered saline and the ALLO/SVF group received 100 μL SVF (2.25 ± 0.45 × 10(7) cells) locally where the grafting was performed.
  
  
  RESULTS
  Behavioral, functional, biomechanical, and gastrocnemius muscle mass showed earlier regeneration of axons in ALLO/SVF than in ALLO group (p < .05). Histomorphometic and immunohistochemical studies also showed earlier regeneration of axons in ALLO/SVF than in ALLO group (p < .05).
  
  
  CONCLUSIONS
  Administration of nonexpanded SVF could accelerate functional recovery after nerve allografting in sciatic nerve. It may have clinical implications for the surgical management of patients after nerve transection.
authors.affiliation: a Department of Surgery and Diagnostic Imaging, Faculty of Veterinary Medicine , Urmia University , Urmia , Iran.
authors.forename: Rahim
authors.initials: R
authors.lastname: Mohammadi
authors.affiliation: a Department of Surgery and Diagnostic Imaging, Faculty of Veterinary Medicine , Urmia University , Urmia , Iran.
authors.forename: Moein
authors.initials: M
authors.lastname: Mehrtash
authors.affiliation: a Department of Surgery and Diagnostic Imaging, Faculty of Veterinary Medicine , Urmia University , Urmia , Iran.
authors.forename: Moeid
authors.initials: M
authors.lastname: Mehrtash
authors.affiliation: b Department of Clinical Sciences, Faculty of Veterinary Medicine , University of Tehran , Tehran , Iran.
authors.forename: Seyedeh-Sepideh
authors.initials: SS
authors.lastname: Sajjadi
mesh_terms: D000273:Adipose Tissue / Q000098:blood supply / Q000166:cytology / Q000637:transplantation*; D064591:Allografts / Q000637:transplantation*; D000818:Animals; D002454:Cell Differentiation; D004195:Disease Models, Animal; D006801:Humans; D008297:Male; D039902:Multipotent Stem Cells / Q000637:transplantation*; D018482:Muscle, Skeletal / Q000601:surgery; D009416:Nerve Regeneration; D059348:Peripheral Nerve Injuries / Q000601:surgery*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D020127:Recovery of Function; D012583:Schwann Cells / Q000502:physiology; D012584:Sciatic Nerve / Q000293:injuries; D017154:Stromal Cells; D014184:Transplantation, Homologous / Q000191:economics / Q000379:methods
keywords: Peripheral nerve repair; SVF; allograft; local; rat; sciatic