title: Effect of CYP1A1 and GSTM1 genetic polymorphisms on bone tumor susceptibility.
abstract: Tumor gene polymorphisms are often associated with individual susceptibility to genetic diseases. Cytochrome P4501A1 (CYP1A1) and glutathione S-transferase mu 1 (GSTM1) gene polymorphisms are closely related to the susceptibility of the body to chemical carcinogens in the environment. Therefore, we explored the relationship between CYP1A1 and GSTM1 gene polymorphisms and susceptibility to bone tumors. Multiplex-polymerase chain reaction (PCR), allelic-specific PCR, and PCR-restriction fragment length polymorphism techniques were used to analyze CYP1A1 and GSTM1 gene polymorphisms in 52 bone tumor patients and 100 healthy subjects. The allelic variation frequency of the CYP1A1 gene at exon 7 (Ile 462 Val) in bone tumor patients was 0.462, which was significantly higher than that in the normal controls (0.223). The frequency of the absence of the GSTM1 homozygous genotype in the patients (0.65) was also markedly higher than that in the control group (0.41). Subjects with CYP1A1 Val/Val homozygous mutations and absence of the GSTM1 homozygous genotype were at markedly increased risk of developing bone tumors [ORs 4.15 (95%CI: 1.268-13.30) and 2.35 (95%CI: 1.15-4.85), respectively]. The OR for the combined effect of the CYP1A1 and GSTM1 gene polymorphisms was 8.55 (95%CI: 1.75-41.50). CYP1A1 and GSTM1 polymorphisms are genetic risk factors in patients with bone tumors, and the allelic variation of these genes increases the risk of bone tumor occurrence.
authors.affiliation: Division of Bone and Joint Surgery, Jinan Central Hospital Affiliated to Shandong University, Jinan, China.
authors.forename: L
authors.initials: L
authors.lastname: Li
authors.affiliation: Division of Bone and Joint Surgery, Zhangqiu People's Hospital, Jinan, Shandong, China.
authors.forename: J G
authors.initials: JG
authors.lastname: Li
authors.affiliation: Division of Bone and Joint Surgery, Zhangqiu People's Hospital, Jinan, Shandong, China.
authors.forename: C Y
authors.initials: CY
authors.lastname: Liu
authors.affiliation: Division of Bone and Joint Surgery, Jinan Central Hospital Affiliated to Shandong University, Jinan, China.
authors.forename: Y J
authors.initials: YJ
authors.lastname: Ding
mesh_terms: D001859:Bone Neoplasms / Q000235:genetics*; D016022:Case-Control Studies; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics*; D005091:Exons; D005787:Gene Frequency; D005982:Glutathione Transferase / Q000235:genetics*; D006720:Homozygote; D006801:Humans; D012516:Osteosarcoma / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide
chemical_list: D019363:Cytochrome P-450 CYP1A1; D005982:Glutathione Transferase; C569463:CYP1A1 protein, human