title: Targeted mutagenesis of an odorant receptor co-receptor using TALEN in Ostrinia furnacalis.
abstract: Genome editing using transcription activator-like effector nuclease (TALEN) has been applied for various model organisms but not yet for agricultural pest insects. In this study, TALEN-mediated mutagenesis of the gene encoding odorant receptor co-receptor (Orco) of an important agricultural pest Ostrinia furnacalis (OfurOrco) was carried out. Of the two pairs of TALEN constructs designed, one generated somatic and germline mutations at rates of 70.8% and 20.8%, respectively. Physiological and behavioral analyses using a gas chromatograph-electroantennographic detector system and a wind tunnel, respectively, revealed that antennal responses to sex pheromone components were decreased to trace levels, and behavioral responses were abolished in OfurOrco mutants. This study demonstrated that TALEN-mediated mutagenesis is applicable to pest insects, and these results will open the way for a better understanding of chemosensory systems in wild insects.
authors.affiliation: Department of Agricultural and Environmental Biology, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
authors.forename: Bin
authors.initials: B
authors.lastname: Yang
authors.affiliation: Department of Agricultural and Environmental Biology, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Fujii
authors.affiliation: Department of Agricultural and Environmental Biology, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
authors.forename: Yukio
authors.initials: Y
authors.lastname: Ishikawa
authors.affiliation: Department of Agricultural and Environmental Biology, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan. Electronic address: matsuo@utlae.org.
authors.forename: Takashi
authors.initials: T
authors.lastname: Matsuo
mesh_terms: D000818:Animals; D004720:Endonucleases / Q000378:metabolism*; D018095:Germ-Line Mutation; D007915:Lepidoptera / Q000378:metabolism*; D016296:Mutagenesis; D018035:Receptors, Odorant / Q000235:genetics* / Q000378:metabolism
chemical_list: D018035:Receptors, Odorant; D004720:Endonucleases
keywords: Genome editing; Knock out; Lepidoptera; Mutant; Non-model insect