title: Asymmetric real-time PCR and multiplex melting curve analysis with TaqMan probes for detecting PIK3CA mutations.
abstract: The data in this article are related to the research article entitled "Optimization of melting analysis with TaqMan probes for detection of KRAS, NRAS, and BRAF mutations" Botezatu et al. [1]. Somatic mutations in the PIK3CA gene ("hot spots" in exons 9 and 20) are found in many human cancers, and their presence can determine prognosis and a treatment strategy. An effective method of mutation scanning PIK3CA in clinical laboratories is DNA Melting Analysis (DMA) (Vorkas et al., 2010; Simi et al., 2008) [2], [3]. It was demonstrated recently that the TaqMan probes which have been long used in Real Time PCR may also be utilized in DMA (Huang et al., 2011) [4]. After optimization of this method Botezatu et al. [1], it was used for multiplex scanning PIK3CA hotspot mutations in formalin-fixed paraffin-embedded (FFPE) samples from patients with colorectal and lung cancer.
authors.affiliation: N.N. Blokhin Russian Cancer Research Center, Kashirskoe Shosse 24, 115478 Moscow, Russia.
authors.forename: Irina V
authors.initials: IV
authors.lastname: Botezatu
authors.affiliation: N.N. Blokhin Russian Cancer Research Center, Kashirskoe Shosse 24, 115478 Moscow, Russia.
authors.forename: Irina O
authors.initials: IO
authors.lastname: Nechaeva
authors.affiliation: N.N. Blokhin Russian Cancer Research Center, Kashirskoe Shosse 24, 115478 Moscow, Russia.
authors.forename: Аnna М
authors.initials: АМ
authors.lastname: Stroganova
authors.affiliation: N.N. Blokhin Russian Cancer Research Center, Kashirskoe Shosse 24, 115478 Moscow, Russia.
authors.forename: Anastasia I
authors.initials: AI
authors.lastname: Senderovich
authors.affiliation: N.N. Blokhin Russian Cancer Research Center, Kashirskoe Shosse 24, 115478 Moscow, Russia.
authors.forename: Valentina N
authors.initials: VN
authors.lastname: Kondratova
authors.affiliation: N.N. Blokhin Russian Cancer Research Center, Kashirskoe Shosse 24, 115478 Moscow, Russia.
authors.forename: Valery P
authors.initials: VP
authors.lastname: Shelepov
authors.affiliation: N.N. Blokhin Russian Cancer Research Center, Kashirskoe Shosse 24, 115478 Moscow, Russia.
authors.forename: Anatoly V
authors.initials: AV
authors.lastname: Lichtenstein
keywords: DNA melting analysis; Multiplex analysis; Mutation scanning; PIK3CA; TaqMan probes