title: Luteolin Exerts Cardioprotective Effects through Improving Sarcoplasmic Reticulum Ca(2+)-ATPase Activity in Rats during Ischemia/Reperfusion In Vivo.
abstract: The flavonoid luteolin exists in many types of fruits, vegetables, and medicinal herbs. Our previous studies have demonstrated that luteolin reduced ischemia/reperfusion (I/R) injury in vitro, which was related with sarcoplasmic reticulum Ca(2+)-ATPase (SERCA2a) activity. However, the effects of luteolin on SERCA2a activity during I/R in vivo remain unclear. To investigate whether luteolin exerts cardioprotective effects and to monitor changes in SERCA2a expression and activity levels in vivo during I/R, we created a myocardial I/R rat model by ligating the coronary artery. We demonstrated that luteolin could reduce the myocardial infarct size, lactate dehydrogenase release, and apoptosis during I/R injury in vivo. Furthermore, we found that luteolin inhibited the I/R-induced decrease in SERCA2a activity in vivo. However, neither I/R nor luteolin altered SERCA2a expression levels in myocardiocytes. Moreover, the PI3K/Akt signaling pathway played a vital role in this mechanism. In conclusion, the present study has confirmed for the first time that luteolin yields cardioprotective effects against I/R injury by inhibiting the I/R-induced decrease in SERCA2a activity partially via the PI3K/Akt signaling pathway in vivo, independent of SERCA2a protein level regulation. SERCA2a activity presents a novel biomarker to assess the progress of I/R injury in experimental research and clinical applications.
authors.affiliation: Institute of Cardiovascular Disease, Xuzhou Medical College, Xuzhou, Jiangsu 221002, China.
authors.forename: Changsheng
authors.initials: C
authors.lastname: Nai
authors.affiliation: Department of Cardiology, The Affiliated Hospital of Xuzhou Medical College, Xuzhou, Jiangsu 221002, China.
authors.forename: Haochen
authors.initials: H
authors.lastname: Xuan
authors.affiliation: Institute of Cardiovascular Disease, Xuzhou Medical College, Xuzhou, Jiangsu 221002, China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Institute of Cardiovascular Disease, Xuzhou Medical College, Xuzhou, Jiangsu 221002, China.
authors.forename: Mengxiao
authors.initials: M
authors.lastname: Shen
authors.affiliation: Department of Cardiology, The Affiliated Hospital of Xuzhou Medical College, Xuzhou, Jiangsu 221002, China.
authors.forename: Tongda
authors.initials: T
authors.lastname: Xu
authors.affiliation: Department of Cardiology, The Affiliated Hospital of Xuzhou Medical College, Xuzhou, Jiangsu 221002, China.
authors.forename: Defeng
authors.initials: D
authors.lastname: Pan
authors.affiliation: Institute of Cardiovascular Disease, Xuzhou Medical College, Xuzhou, Jiangsu 221002, China.
authors.forename: Congwei
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Cardiology, The Affiliated Hospital of Xuzhou Medical College, Xuzhou, Jiangsu 221002, China.
authors.forename: Yanbin
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Institute of Cardiovascular Disease, Xuzhou Medical College, Xuzhou, Jiangsu 221002, China.
authors.forename: Dongye
authors.initials: D
authors.lastname: Li