title: Identification and characterization of a novel antioxidant peptide from feather keratin hydrolysate.
abstract: OBJECTIVE
  To improve the potential value of feather, which is a valuable protein resource, we have separated and identified antioxidant peptide(s) from feather hydrolysate.
  
  
  RESULTS
  Feather hydrolysate was prepared by fermentation with Bacillus subtilis S1-4. Antioxidative peptides were separated by sequential acid precipitation, cation exchange, and reversed-phase fast performance liquid chromatography. Finally, a peptide with antioxidative activity was identified as Ser-Asn-Leu-Cys-Arg-Pro-Cys-Gly by MALDI time-of-flight (TOF)/TOF analysis, and determined to represent a portion of feather keratin near its N-terminal. A synthesized peptide with the same sequence was used to characterize its antioxidative properties, including scavenging free radicals, reducing power, and Fe(2+) chelation. In terms of the peptide's amino acid composition, the antioxidative activity might be mainly attributed to Cys and other amino acid residues.
  
  
  CONCLUSIONS
  Feather keratin is a good source for the quantitative preparation of antioxidative peptides.
authors.affiliation: Key Laboratory for Bio-Resources and Eco-Environment of Ministry of Education, Sichuan Key Laboratory of Molecular Biology and Biotechnology, College of Life Sciences, Sichuan University, 29 Wangjiang Road, Chengdu, 610064, Sichuan, People's Republic of China.
authors.forename: Min-Yuan
authors.initials: MY
authors.lastname: Wan
authors.affiliation: Key Laboratory for Bio-Resources and Eco-Environment of Ministry of Education, Sichuan Key Laboratory of Molecular Biology and Biotechnology, College of Life Sciences, Sichuan University, 29 Wangjiang Road, Chengdu, 610064, Sichuan, People's Republic of China.
authors.forename: Ge
authors.initials: G
authors.lastname: Dong
authors.affiliation: Key Laboratory for Bio-Resources and Eco-Environment of Ministry of Education, Sichuan Key Laboratory of Molecular Biology and Biotechnology, College of Life Sciences, Sichuan University, 29 Wangjiang Road, Chengdu, 610064, Sichuan, People's Republic of China.
authors.forename: Bin-Qing
authors.initials: BQ
authors.lastname: Yang
authors.affiliation: Key Laboratory for Bio-Resources and Eco-Environment of Ministry of Education, Sichuan Key Laboratory of Molecular Biology and Biotechnology, College of Life Sciences, Sichuan University, 29 Wangjiang Road, Chengdu, 610064, Sichuan, People's Republic of China. hfeng@scu.edu.cn.
authors.forename: Hong
authors.initials: H
authors.lastname: Feng
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D001412:Bacillus subtilis / Q000502:physiology; D002645:Chickens / Q000033:anatomy & histology*; D005241:Feathers / Q000737:chemistry* / Q000382:microbiology*; D005285:Fermentation; D006868:Hydrolysis; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D000975:Antioxidants; D010455:Peptides
keywords: Antioxidative peptide; Bacillus subtilis; Degradation; Feather; Hydrolysate; Keratin