title: Enhanced ELISA using a handheld pH meter and enzyme-coated microparticles for the portable, sensitive detection of proteins.
abstract: This work describes a general methodology for enhanced enzyme-linked immunosorbent assay (ELISA) that integrates enzyme-coated microparticle probes for robust yet highly efficient signal amplification and a handheld pH meter for a simple, portable, quantitative readout. Its utility is well demonstrated with the detection of the target protein with a 14-fold enhancement of sensitivity in comparison with the conventional optical ELISA.
authors.affiliation: Guangxi Key Laboratory of Electrochemical and Magnetochemical Function Materials, College of Chemistry and Bioengineering, Guilin University of Technology, Guilin 541004, P. R. China. zy@glut.edu.cn Niejinfang@glut.edu.cn.
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhang
authors.forename: Jiani
authors.initials: J
authors.lastname: Yang
authors.forename: Jinfang
authors.initials: J
authors.lastname: Nie
authors.forename: Juanhua
authors.initials: J
authors.lastname: Yang
authors.forename: Dong
authors.initials: D
authors.lastname: Gao
authors.forename: Lang
authors.initials: L
authors.lastname: Zhang
authors.forename: Jianping
authors.initials: J
authors.lastname: Li
mesh_terms: D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005949:Glucose Oxidase / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D015335:Molecular Probes; D011506:Proteins / Q000032:analysis*
chemical_list: D015335:Molecular Probes; D011506:Proteins; D005949:Glucose Oxidase