title: The effect of alpha-tocopheryl succinate on succinate respiration in rat liver mitochondria.
abstract: We compared the effect of alpha-tocopheryl succinate (TOS) on succinate-dependent respiration in rat liver mitochondria, homogenate and permeabilized hepatocytes in both a coupled and uncoupled state. In isolated mitochondria, a significant inhibitory effect was observed at a concentration of 5 microM, in liver homogenate at 25 microM and in permeabilized hepatocytes at 50 microM. The inhibitory effect of TOS on succinate respiration in an uncoupled state was less pronounced than in a coupled state in all the experimental models tested. When the concentration dependence of the TOS inhibitory effect was tested, the most sensitive in both states were isolated mitochondria; the most resistant were permeabilized hepatocytes.
authors.affiliation: Department of Physiology, Charles University in Prague, Faculty of Medicine in Hradec Králové, Czech Republic. wolff@lfhk.cuni.cz.
authors.forename: O
authors.initials: O
authors.lastname: Sobotka
authors.forename: Z
authors.initials: Z
authors.lastname: Drahota
authors.forename: O
authors.initials: O
authors.lastname: Kučera
authors.forename: R
authors.initials: R
authors.lastname: Endlicher
authors.forename: H
authors.initials: H
authors.lastname: Rauchová
authors.forename: Z
authors.initials: Z
authors.lastname: Červinková
mesh_terms: D000818:Animals; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone / Q000494:pharmacology; D002462:Cell Membrane / Q000378:metabolism; D002463:Cell Membrane Permeability; D019069:Cell Respiration / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D042963:Electron Transport Complex II / Q000378:metabolism; D004734:Energy Metabolism / Q000187:drug effects*; D022781:Hepatocytes / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000378:metabolism; D010085:Oxidative Phosphorylation / Q000187:drug effects; D017208:Rats, Wistar; D013997:Time Factors; D014475:Uncoupling Agents / Q000494:pharmacology; D024502:alpha-Tocopherol / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D014475:Uncoupling Agents; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone; D042963:Electron Transport Complex II; D024502:alpha-Tocopherol