title: Effect of chito-oligosaccharides over human faecal microbiota during fermentation in batch cultures.
abstract: Chitosan with high number of deacetylated units, its reacetylated derivative and COS obtained through an enzymatic treatment with chitosanase were tested in pH controlled batch cultures to investigate the ability of the human faecal microbiota to utilise them. Chitosan derivatives with high number of deacetylated units decreased the bacterial populations: Bifidobacterium spp., Eubacterium rectale/Clostridium coccoides, C. Histolyticum and Bacteroides/Prevotella. On the other hand, chitosan derivatives with high content of acetylated residues maintained the tested bacterial groups and could increase Lactobacillus/Enterococcus. Regarding short chain fatty acids (SCFA), only low Mw COS increased the production in similar levels than fructo-oligossacharides (FOS). The acetylated chitosans and their COS do not appear as potential prebiotics but did not affect negatively the faecal microbiota, while derivatives with high number of deacetylated units could induce a colonic microbiota imbalance.
authors.affiliation: Departamento de Nutrición y Bromatología II, Facultad de Farmacia, Universidad Complutense de Madrid, Plaza Ramón y Cajal s/n, 28040 Madrid, Spain. Electronic address: inmateos@ucm.es.
authors.forename: Inmaculada
authors.initials: I
authors.lastname: Mateos-Aparicio
authors.affiliation: Instituto de Estudios Biofuncionales, Departamento de Química Física II, Facultad de Farmacia, Universidad Complutense de Madrid, 28040 Madrid, Spain.
authors.forename: Marian
authors.initials: M
authors.lastname: Mengíbar
authors.affiliation: Instituto de Estudios Biofuncionales, Departamento de Química Física II, Facultad de Farmacia, Universidad Complutense de Madrid, 28040 Madrid, Spain. Electronic address: aheras@ucm.es.
authors.forename: Angeles
authors.initials: A
authors.lastname: Heras
mesh_terms: D061249:Batch Cell Culture Techniques; D048271:Chitosan / Q000737:chemistry*; D005243:Feces / Q000382:microbiology*; D005285:Fermentation; D006801:Humans; D008970:Molecular Weight; D009844:Oligosaccharides / Q000737:chemistry*
chemical_list: D009844:Oligosaccharides; D048271:Chitosan
keywords: Chito-oligosaccharides; Chitosan; Fermentation; Microbiota; Prebiotic