title: Comparison of pinoresinol diglucoside production by Phomopsis sp. XP-8 in different media and the characterisation and product profiles of the cultivation in mung bean.
abstract: BACKGROUND
  Phomopsis sp. XP-8 is an endophytic fungus with the ability to produce pinoresinol diglucoside (PDG) in vitro and thus has potential application in biosynthesis of PDG independent of plants. In order to enhance the production of PDG, 18 different natural materials were tested in solid-state cultivation of Phomopsis sp. XP-8.
  
  
  RESULTS
  Most of the tested natural materials promoted the production of PDG. A supplement derived from mung beans produced the highest PDG yield and better fungal growth than the other materials. Also, pinoresinol monoglucoside, pinoresinol and other substrates (phenylalanine, p-coumaric acid, cinnamic acid, caffeic acid, and ferulic acid) were obtained after fermentation on mung beans. Furthermore, PDG production was much higher when mung beans were incorporated into solid state agar versus a liquid medium. The highest pinoresinol diglucoside production (72.1 mg kg(-1) in fresh culture) was obtained in 9 days using a solid state culture of Phomopsis sp. XP-8 on a mung bean grain medium containing 100 g kg(-1) glucose. Mung bean water-soluble polysaccharide was identified as a major promoter of PDG production by Phomopsis sp. XP-8.
  
  
  CONCLUSIONS
  Mung bean, especially its water-soluble polysaccharide fraction, was an efficient natural material to promote PDG production by Phomopsis sp. XP-8. © 2015 Society of Chemical Industry.
authors.affiliation: College of Food Science and Engineering, Northwest A&F University, 28 Xinong Road, Yangling, Shaanxi Province, 712100, China.
authors.forename: Yan
authors.identifier: http://orcid.org/0000-0002-6296-3588
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, 127 Youyi West Road, Xi'an, Shaanxi Province, 710072, China.
authors.forename: Junling
authors.initials: J
authors.lastname: Shi
authors.affiliation: College of Food Science and Engineering, Northwest A&F University, 28 Xinong Road, Yangling, Shaanxi Province, 712100, China.
authors.forename: Zhenhong
authors.initials: Z
authors.lastname: Gao
authors.affiliation: College of Food Science and Engineering, Northwest A&F University, 28 Xinong Road, Yangling, Shaanxi Province, 712100, China.
authors.forename: Jinxin
authors.initials: J
authors.lastname: Che
authors.affiliation: Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, 127 Youyi West Road, Xi'an, Shaanxi Province, 710072, China.
authors.forename: Dongyan
authors.initials: D
authors.lastname: Shao
authors.affiliation: College of Enology, Northwest A&F University, Xinong Road, Yangling, Shaanxi Province, 712100, China.
authors.forename: Yanlin
authors.initials: Y
authors.lastname: Liu
mesh_terms: D001203:Ascomycota / Q000378:metabolism*; D003470:Culture Media; D005516:Food Microbiology; D006801:Humans; D017705:Lignans / Q000096:biosynthesis*; D000070658:Vigna / Q000254:growth & development / Q000378:metabolism*
chemical_list: D003470:Culture Media; D017705:Lignans; C013200:pinoresinol diglucoside
keywords: Phomopsis sp.; mung bean; pinoresinol diglucoside; solid-state cultivation