title: A honeycomb composite of mollusca shell matrix and calcium alginate.
abstract: A honeycomb composite is useful to carry cells for application in bone, cartilage, skin, and soft tissue regenerative therapies. To fabricate a composite, and expand the application of mollusca shells as well as improve preparing methods of calcium alginate in tissue engineering research, Anodonta woodiana shell powder was mixed with sodium alginate at varying mass ratios to obtain a gel mixture. The mixture was frozen and treated with dilute hydrochloric acid to generate a shell matrix/calcium alginate composite. Calcium carbonate served as the control. The composite was transplanted subcutaneously into rats. At 7, 14, 42, and 70 days after transplantation, frozen sections were stained with hematoxylin and eosin, followed by DAPI, β-actin, and collagen type-I immunofluorescence staining, and observed using laser confocal microscopy. The composite featured a honeycomb structure. The control and composite samples displayed significantly different mechanical properties. The water absorption rate of the composite and control group were respectively 205-496% and 417-586%. The composite (mass ratio of 5:5) showed good biological safety over a 70-day period; the subcutaneous structure of the samples was maintained and the degradation rate was lower than that of the control samples. Freezing the gel mixture afforded control over chemical reaction rates. Given these results, the composite is a promising honeycomb scaffold for tissue engineering.
authors.affiliation: Key Laboratory of Freshwater Fish Reproduction and Development (Southwest University), Ministry of Education, Chongqing 400715, China; School of Life Science, Southwest University, Chongqing 400715, China.
authors.forename: Hua-jian
authors.initials: HJ
authors.lastname: You
authors.affiliation: Key Laboratory of Freshwater Fish Reproduction and Development (Southwest University), Ministry of Education, Chongqing 400715, China; School of Life Science, Southwest University, Chongqing 400715, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Li
authors.affiliation: Chongqing Academy of Animal Science, Chongqing 402460, China.
authors.forename: Chan
authors.initials: C
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Freshwater Fish Reproduction and Development (Southwest University), Ministry of Education, Chongqing 400715, China; School of Life Science, Southwest University, Chongqing 400715, China. Electronic address: xytom@swu.edu.cn.
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
authors.affiliation: Key Laboratory of Freshwater Fish Reproduction and Development (Southwest University), Ministry of Education, Chongqing 400715, China; School of Life Science, Southwest University, Chongqing 400715, China.
authors.forename: Yao-guang
authors.initials: YG
authors.lastname: Zhang
mesh_terms: D000199:Actins / Q000235:genetics / Q000378:metabolism; D000464:Alginates / Q000737:chemistry / Q000494:pharmacology*; D060105:Animal Shells / Q000737:chemistry*; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D024042:Collagen Type I / Q000235:genetics / Q000378:metabolism; D055119:Elastic Modulus; D005260:Female; D015870:Gene Expression; D020723:Glucuronic Acid / Q000737:chemistry / Q000494:pharmacology; D006603:Hexuronic Acids / Q000737:chemistry / Q000494:pharmacology; D006851:Hydrochloric Acid / Q000737:chemistry; D020100:Hydrogels / Q000138:chemical synthesis / Q000494:pharmacology*; D007279:Injections, Subcutaneous; D008297:Male; D008974:Mollusca / Q000737:chemistry; D011208:Powders / Q000737:chemistry / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013718:Tensile Strength; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D000199:Actins; D000464:Alginates; D015415:Biomarkers; D024042:Collagen Type I; D006603:Hexuronic Acids; D020100:Hydrogels; D011208:Powders; D020723:Glucuronic Acid; D006851:Hydrochloric Acid
keywords: Anodonta woodiana; Calcium alginate; Matrix; Shell; Sodium alginate