title: Comparative investigation of the binding characteristics of poly-L-lysine and chitosan on alginate hydrogel.
abstract: The binding properties of poly-L-lysine and chitosan to alginate have been evaluated quantitatively and compared. Poly-L-lysine bound to alginate hydrogel more rapidly than chitosan as poly-L-lysine has a smaller molar hydrodynamic volume. In addition, poly-L-lysine showed a much higher binding capacity (6.14:1) for alginate hydrogel beads than chitosan (2.71:1), and a little higher binding stoichiometry (0.58) to sodium alginate molecules in solution than chitosan (0.49). An exothermic heat of alginate-poly-L-lysine complexes formation of 2.02 kJ/mol was detected. For alginate-chitosan complexes, the binding enthalpy has been seen to be -3.49 kJ/mol. The stability of the polyelectrolyte complexes was related to their binding enthalpy. The alginate-poly-L-lysine complexes could be disintegrated and rebuilt. By contrast, chitosan was bound with alginate in a steady state. These results provide fundamental insights regarding the structure and property relationships of macromolecules, and will be helpful in designing and selecting appropriate polymers.
authors.affiliation: Laboratory of Biomedical Material Engineering, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Ren
authors.affiliation: Laboratory of Biomedical Material Engineering, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China. Electronic address: xiehg@dicp.ac.cn.
authors.forename: Hongguo
authors.initials: H
authors.lastname: Xie
authors.affiliation: Laboratory of Biomedical Material Engineering, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Xiaocen
authors.initials: X
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Bao
authors.affiliation: Laboratory of Biomedical Material Engineering, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China. Electronic address: yuwt@dicp.ac.cn.
authors.forename: Weiting
authors.initials: W
authors.lastname: Yu
authors.affiliation: Laboratory of Biomedical Material Engineering, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Ma
mesh_terms: D000464:Alginates / Q000737:chemistry*; D002214:Capsules / Q000737:chemistry; D048271:Chitosan / Q000737:chemistry*; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D055595:Mechanical Phenomena; D011073:Polyamines / Q000737:chemistry; D000071228:Polyelectrolytes; D011107:Polylysine / Q000737:chemistry*
chemical_list: D000464:Alginates; D002214:Capsules; D048271:Chitosan; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D011073:Polyamines; D000071228:Polyelectrolytes; D011107:Polylysine
keywords: Alginate; Chitosan; Poly-l-lysine