title: Isotope dilution gas chromatography with mass spectrometry for the analysis of 4-octyl phenol, 4-nonylphenol, and bisphenol A in vegetable oils.
abstract: By the combination of solid-phase extraction as well as isotope dilution gas chromatography with mass spectrometry, a sensitive and reliable method for the determination of endocrine-disrupting chemicals including bisphenol A, 4-octylphenol, and 4-nonylphenol in vegetable oils was established. The application of a silica/N-(n-propyl)ethylenediamine mixed solid-phase extraction cartridge achieved relatively low matrix effects for bisphenol A, 4-octylphenol, and 4-nonylphenol in vegetable oils. Experiments were designed to evaluate the effects of derivatization, and the extraction parameters were optimized. The estimated limits of detection and quantification for bisphenol A, 4-octylphenol, and 4-nonylphenol were 0.83 and 2.5 μg/kg, respectively. In a spiked experiment in vegetable oils, the recovery of the added bisphenol A was 97.5-110.3%, recovery of the added 4-octylphenol was 64.4-87.4%, and that of 4-nonylphenol was 68.2-89.3%. This sensitive method was then applied to real vegetable oil samples from Zhejiang Province of China, and none of the target compounds were detected.
authors.affiliation: Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, Zhejiang, P.R. China.
authors.forename: Pinggu
authors.initials: P
authors.lastname: Wu
authors.affiliation: Hangzhou Center for Disease Control and Prevention, Hangzhou, P.R. China.
authors.forename: Liqun
authors.initials: L
authors.lastname: Zhang
authors.affiliation: China National Center for Food Safety Risk Assessment, Beijing, P.R. China.
authors.forename: Dajin
authors.initials: D
authors.lastname: Yang
authors.affiliation: Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, Zhejiang, P.R. China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, Zhejiang, P.R. China.
authors.forename: Zhengyan
authors.initials: Z
authors.lastname: Hu
authors.affiliation: Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, Zhejiang, P.R. China.
authors.forename: Liyuan
authors.initials: L
authors.lastname: Wang
authors.affiliation: Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, Zhejiang, P.R. China.
authors.forename: Bingjie
authors.initials: B
authors.lastname: Ma
mesh_terms: D001559:Benzhydryl Compounds / Q000032:analysis*; D002681:China; D052244:Endocrine Disruptors / Q000032:analysis; D005506:Food Contamination / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D007201:Indicator Dilution Techniques; D057230:Limit of Detection; D010636:Phenols / Q000032:analysis*; D010938:Plant Oils / Q000737:chemistry*; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D052244:Endocrine Disruptors; D010636:Phenols; D010938:Plant Oils; C080417:4-octylphenol; C041594:4-nonylphenol; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: Alkylphenols; Bisphenol A; Solid-phase extraction; Vegetable oils