title: Relationship between IFNA1, IFNA5, IFNA10, and IFNA17 gene polymorphisms and Crimean-Congo hemorrhagic fever prognosis in a Turkish population range.
abstract: Crimean-Congo hemorrhagic fever (CCHF) is a fatal emerging acute viral infection. Not much is known regarding the pathogenic mechanisms and the reasons behind severe or mild disease courses in CCHF. IFN-alpha (IFNA) is one of the essential cytokines in the immune system. Existence of single nucleotide gene polymorphisms (SNPs) in cytokines can cause susceptibility or resistance to viral agents and different clinical courses. Hence, the relationship between SNPs in genes encoding cytokines (IFNA1 -1823G/A (rs1332190), IFNA5 -2529T/A (rs758236), IFNA10 Cys20stop (rs10119910), and IFNA17 Ile184Arg (rs9298814) SNPs and disease susceptibility were investigated. The associations between SNPs and CCHF prognosis were also studied. Total 150 patients with CCHF and 170 healthy individuals were enrolled. Genotyping was performed by PCR-RFLP methods. The frequency of IFNA1 -1823 (rs1332190) GG genotype was significantly higher in control subjects than CCHF patients (20% vs. 8%; P = 0.01). For IFNA17 Ile184Arg (rs9298814) polymorphism, CCHF patients having TG genotype had a higher frequency than the control subjects (38% vs. 32.4%; P = 0.039). The distribution of TT + TG genotype frequencies was also significantly higher in CCHF group than the controls (97.3% vs. 91.8%; P = 0.049). Genotype and allele frequencies for IFNA subtypes between fatal and survivors were the same (P > 0.05). Genotype and allele frequencies between severe and mild/moderate CCHF patients were also the same (P > 0.05). The results show that IFNA1 rs1332190 and IFNA17 rs9298814 SNPs may play an important role in CCHF susceptibility. Determining the existence of other connections for IFNA SNPs and CCHF severity and fatality requires further investigations.
authors.affiliation: Department of Infectious Diseases and Clinical Microbiology, Faculty of Medicine, Cumhuriyet University, Sivas, Turkey.
authors.forename: Nazif
authors.initials: N
authors.lastname: Elaldi
authors.affiliation: Cumhuriyet University Medical Faculty Research Center (CUTFAM), Cumhuriyet University, Sivas, Turkey.
authors.forename: Meral
authors.initials: M
authors.lastname: Yilmaz
authors.affiliation: Department of Nutrition and Dietetics, Faculty of Health Sciences, Cumhuriyet University, Sivas, Turkey.|Advanced Technology Research and Development Research and Application Center, Cumhuriyet University, Sivas, Turkey.
authors.forename: Binnur
authors.initials: B
authors.lastname: Bagci
authors.affiliation: Department of Medical Biology, Faculty of Medicine, Cumhuriyet University, Sivas, Turkey.
authors.forename: Izzet
authors.initials: I
authors.lastname: Yelkovan
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Cumhuriyet University, Sivas, Turkey.
authors.forename: Gokhan
authors.initials: G
authors.lastname: Bagci
authors.affiliation: Department of Infectious Diseases and Clinical Microbiology, Faculty of Medicine, Cumhuriyet University, Sivas, Turkey.
authors.forename: Mustafa Gokhan
authors.initials: MG
authors.lastname: Gozel
authors.affiliation: Department of Infectious Diseases and Clinical Microbiology, Faculty of Medicine, Cumhuriyet University, Sivas, Turkey.
authors.forename: Aynur
authors.initials: A
authors.lastname: Engin
authors.affiliation: Department of Infectious Diseases and Clinical Microbiology, Faculty of Medicine, Cumhuriyet University, Sivas, Turkey.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Bakir
authors.affiliation: Department of Infectious Diseases and Clinical Microbiology, Sisli Etfal Training and Research Hospital, Istanbul, Turkey.
authors.forename: Ilyas
authors.initials: I
authors.lastname: Dokmetas
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006479:Hemorrhagic Fever, Crimean / Q000453:epidemiology / Q000235:genetics* / Q000276:immunology* / Q000401:mortality; D006801:Humans; D016898:Interferon-alpha / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D011379:Prognosis; D014421:Turkey / Q000453:epidemiology; D055815:Young Adult
chemical_list: C554156:IFNA1 protein, human; C469326:IFNA10 protein, human; C471357:IFNA17 protein, human; C554160:IFNA5 protein, human; D016898:Interferon-alpha
keywords: CCHF; IFNA1; IFNA17; Turkish population range; gene polymorphism