title: Reduction of Leukocyte Counts by Hydroxyurea Improves Cardiac Function in Rats with Acute Myocardial Infarction.
abstract: BACKGROUND This study aimed to decrease leukocytes counts by hydroxyurea (Hu) in an acute myocardial infarction (AMI) rat model and examine its effect on the inflammatory response of myocardial infarction and cardiac functions. MATERIAL AND METHODS AMI was successfully caused in 36 rats, and 12 control rats received sham operation. Rats in the AMI group were then randomly divided into Hu and vehicle group with 18 rats each. Rats in the Hu AMI group received Hu (200 mg/kg) intragastrically while vehicle AMI group received saline. Leukocytes counts, cardiac functions, myocardial tissue morphology, and levels of soluble intercellular adhesion molecule-1 (sICAM), P-selectin and platelet activating factor (PAF) were measured and compared among the three groups four weeks after AMI induction. RESULTS Leukocytes, neutrophils, and leukomonocyte counts in vehicle AMI rats were significantly higher than that of the normal control group (p<0.05). However, Hu treatment decreased their counts significantly (p<0.05). sICAM, P-selectin, and PAF level in vehicle AMI group were significantly higher than those of the normal group, and their level was also decreased by Hu treatment (p<0.05). Echocardiography analysis showed that Hu treatment increased left ventricular ejection fraction (LVEF) and left ventricular fractional shortening (LVFS) compared to that of vehicle AMI group (p<0.05). Histopathological examination showed that Hu significantly reduced the swelling of the heart muscle fiber in necrotic foci and the number of inflammatory cells infiltrated into myocardial interstitium compared to vehicle AMI group. CONCLUSIONS Decrease leukocytes counts by Hu significantly reduced inflammatory reaction and improved cardiac functions in AMI rats.
authors.affiliation: Department of Cardiology, Shandong Provincial Hospital Affiliated to Shandong University, Ji'nan, Shandong, China (mainland).
authors.forename: Guiyue
authors.initials: G
authors.lastname: Zhu
authors.affiliation: Department of Cardiology, Shandong Provincial Qianfoshan Hospital, Shandong University, Ji'nan, Shandong, China (mainland).
authors.forename: Yucai
authors.initials: Y
authors.lastname: Yao
authors.affiliation: Department of Emergency Medicine, Second People's Hospital of Liaocheng, Linqing, Shandong, China (mainland).
authors.forename: Lingyun
authors.initials: L
authors.lastname: Pan
authors.affiliation: Institute of Radiation Medicine, Shandong Academy of Medical Sciences, Ji'nan, Shandong, China (mainland).
authors.forename: Wei
authors.initials: W
authors.lastname: Zhu
authors.affiliation: Department of Cardiology, Shandong Provincial Qianfoshan Hospital, Shandong University, Ji'nan, Shandong, China (mainland).
authors.forename: Suhua
authors.initials: S
authors.lastname: Yan
mesh_terms: D000818:Animals; D004452:Echocardiography; D004562:Electrocardiography; D006334:Heart Function Tests / Q000187:drug effects*; D006918:Hydroxyurea / Q000494:pharmacology*; D007958:Leukocyte Count; D008297:Male; D009203:Myocardial Infarction / Q000000981:diagnostic imaging / Q000503:physiopathology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D006918:Hydroxyurea