title: A high performance thin layer chromatographic method for the estimation of colchicine in different formulations.
abstract: BACKGROUND
  Colchicine is a main alkaloid present in bitter and sweet variety of colchicum (Colchicum luteum Baker), which have been reported to possess anti-rheumatic, anti-gout, and anticancer potential. Colchicum is an important ingredient of several Unani and Herbal formulations. Quantification of colchicine will play a great role in quality control of these formulations. Hence, a high-performance thin layer chromatographic (TLC) method has been developed for the analysis of colchicine in Unani formulations of various dosage forms such as hubb (tablet) and capsules.
  
  
  METHODS
  The samples were applied on aluminum TLC plates precoated with silica gel 60-F254 and developed using mobile phase toluene-dichloromethane-methanol in equal proportions. Quantification was done by densitometric scanning at 350 nm, which showed a linear response in the range of 50-500 ng/spot. The developed method was validated as per the International Conference on Harmonization guidelines for linearity, precision, accuracy, specificity, robustness, limit of detection, and limit of quantification.
  
  
  CONCLUSIONS
  The developed method was applied for quantitative estimation of colchicine in different Unani and Herbal formulations. The method was found simple, selective, accurate with a wide range of linearity, hence suitable for the quality control of different formulations and varieties of colchicum with respect to colchicine content.
authors.affiliation: Nandini Nagar College of Pharmacy, Nawabganj, Gonda, Uttar Pradesh, India.
authors.forename: Mohd
authors.initials: M
authors.lastname: Fahim
authors.affiliation: Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Bioactive Natural Product Laboratory, Hamdard University, New Delhi, India.
authors.forename: Mhaveer
authors.initials: M
authors.lastname: Singh
authors.affiliation: Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Bioactive Natural Product Laboratory, Hamdard University, New Delhi, India.
authors.forename: Y T
authors.initials: YT
authors.lastname: Kamal
authors.affiliation: SBS College of Pharmacy, Patti, Punjab, India.
authors.forename: Hayat M
authors.initials: HM
authors.lastname: Mukhtar
authors.affiliation: Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Bioactive Natural Product Laboratory, Hamdard University, New Delhi, India.
authors.forename: Sayeed
authors.initials: S
authors.lastname: Ahmad
keywords: Colchicine; Colchicum luteum; estimation; high-performance thin layer chromatographic; validation