title: Direct utilization of waste water algal biomass for ethanol production by cellulolytic Clostridium phytofermentans DSM1183.
abstract: Direct bioconversion of waste water algal biomass into ethanol using Clostridium phytofermentans DSM1183 was demonstrated in this study. Fermentation of 2% (w/v) autoclaved algal biomass produced ethanol concentration of 0.52 g L(-1) (solvent yield of 0.19 g/g) where as fermentation of acid pretreated algal biomass (2%, w/v) produced ethanol concentration of 4.6 g L(-1) in GS2 media (solvent yield of 0.26 g/g). The control experiment with 2% (w/v) glucose in GS2 media produced ethanol concentration of 2.8 g L(-1) (solvent yield of 0.25 g/g). The microalgal strains from waste water algal biomass were identified as Chlamydomonas dorsoventralis, Graesiella emersonii, Coelastrum proboscideum, Scenedesmus obliquus, Micractinium sp., Desmodesmus sp., and Chlorella sp., based on ITS-2 molecular marker. The presence of glucose, galactose, xylose and rhamnose were detected by high performance liquid chromatography in the algal biomass. Scanning Electron Microscopy observations of fermentation samples showed characteristic morphological changes in algal cells and bioaccessibility of C. phytofermentans.
authors.affiliation: Molecular Genetics Lab, Department of Genetic Engineering, School of Bioengineering, SRM University, Kattankulathur, Kancheepuram District, Tamil Nadu 603203, India.
authors.forename: Anwar Aliya
authors.initials: AA
authors.lastname: Fathima
authors.affiliation: Molecular Genetics Lab, Department of Genetic Engineering, School of Bioengineering, SRM University, Kattankulathur, Kancheepuram District, Tamil Nadu 603203, India.
authors.forename: Mary
authors.initials: M
authors.lastname: Sanitha
authors.affiliation: Analytical Research and Development Division, Piramal Enterprises Limited, Chennai 600057, India.
authors.forename: Thangarathinam
authors.initials: T
authors.lastname: Kumar
authors.affiliation: Molecular Genetics Lab, Department of Genetic Engineering, School of Bioengineering, SRM University, Kattankulathur, Kancheepuram District, Tamil Nadu 603203, India.
authors.forename: Sellamuthu
authors.initials: S
authors.lastname: Iyappan
authors.affiliation: Molecular Genetics Lab, Department of Genetic Engineering, School of Bioengineering, SRM University, Kattankulathur, Kancheepuram District, Tamil Nadu 603203, India. Electronic address: ramya.m@ktr.srmuniv.ac.in.
authors.forename: Mohandass
authors.initials: M
authors.lastname: Ramya
mesh_terms: D001673:Biodegradation, Environmental; D018533:Biomass; D002482:Cellulose / Q000378:metabolism; D000460:Chlorophyta / Q000737:chemistry / Q000378:metabolism; D003013:Clostridium / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D006868:Hydrolysis; D058086:Microalgae / Q000737:chemistry* / Q000378:metabolism*; D012210:Rhamnose / Q000378:metabolism; D062065:Wastewater / Q000737:chemistry*; D014994:Xylose / Q000378:metabolism
chemical_list: D002482:Cellulose; D000431:Ethanol; D005947:Glucose; D012210:Rhamnose; D062065:Wastewater; D014994:Xylose
keywords: Batch fermentation; Clostridium phytofermentans; Ethanol; Microalgal biomass