title: miR-sc8 Inhibits Schwann Cell Proliferation and Migration by Targeting Egfr.
abstract: MicroRNAs (miRNAs) negatively regulate the expression of target genes at the post-transcriptional level in diverse biological processes. We have previously identified a group of novel miRNAs in proximal nerve following rat sciatic nerve transection by Solexa sequencing. In this study, the biological function and action mode of miR-sc8, one of the above identified miRNAs, were investigated. An increased expression of miR-sc8 inhibited cell proliferation and migration of Schwann cells (SCs), and inversely, silencing of the miR-sc8 expression promoted cell proliferation and migration of SCs. The epidermal growth factor receptor (Egfr) was identified as the target gene of miR-sc8, which exerted negative regulation of Egfr by translational suppression. The temporal change profile of the miR-sc8 expression was negatively correlated with that of the Egfr expression in proximal nerve following sciatic nerve transection. Moreover, Knockdown of Egfr attenuated the promoting effects of miR-sc8 inhibitor on SC proliferation and migration. Overall, our data indicate that miR-sc8 affects phenotype modulation of SCs by targeting Egfr, providing further insights into the regulatory role of miRNAs in peripheral nerve regeneration.
authors.affiliation: Jiangsu Key Laboratory of Neuroregeneration, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu, P. R. China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Gu
authors.affiliation: Jiangsu Key Laboratory of Neuroregeneration, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu, P. R. China.
authors.forename: Chu
authors.initials: C
authors.lastname: Chen
authors.affiliation: Jiangsu Key Laboratory of Neuroregeneration, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu, P. R. China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Yi
authors.affiliation: Department of Obstetrics and Gynecology, Affiliated Hospital of Nantong University, Nantong, Jiangsu, P. R. China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Wang
authors.affiliation: Jiangsu Key Laboratory of Neuroregeneration, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu, P. R. China.
authors.forename: Leilei
authors.initials: L
authors.lastname: Gong
authors.affiliation: Jiangsu Key Laboratory of Neuroregeneration, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu, P. R. China.
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
authors.affiliation: Jiangsu Key Laboratory of Neuroregeneration, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu, P. R. China.
authors.forename: Xiaosong
authors.initials: X
authors.lastname: Gu
authors.affiliation: Key Laboratory of the People's Liberation Army, Institute of Orthopaedics, Chinese PLA General Hospital, Beijing, P. R. China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: Jiangsu Key Laboratory of Neuroregeneration, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu, P. R. China.
authors.forename: Shiying
authors.initials: S
authors.lastname: Li
mesh_terms: D020413:3' Untranslated Regions; D000818:Animals; D002465:Cell Movement; D049109:Cell Proliferation; D002478:Cells, Cultured; D066246:ErbB Receptors / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D012583:Schwann Cells / Q000166:cytology* / Q000378:metabolism
chemical_list: D020413:3' Untranslated Regions; C000608697:MIRNsc8 microRNA, rat; D035683:MicroRNAs; C483803:Egfr protein, rat; D066246:ErbB Receptors