title: Nasopharyngeal Infection of Mice with Streptococcus pyogenes and In Vivo Detection of Superantigen Activity.
abstract: Streptococcus pyogenes is a globally prominent human-specific pathogen that is responsible for an enormous burden of infectious disease. Despite intensive experimental efforts to understand the molecular correlates that contribute to invasive infections, there has been less focus on S. pyogenes carriage and local infection of the nasopharynx. This chapter describes an acute nasopharyngeal infection model in mice that is utilized in our laboratory to study the role of superantigen toxins in the biology of S. pyogenes. We also describe a method to detect superantigen-specific T cell activation in vivo.
authors.affiliation: Department of Microbiology and Immunology, Schulich School of Medicine and Dentistry, Western University, London, ON, Canada, N6A5C1.
authors.forename: Joseph J
authors.initials: JJ
authors.lastname: Zeppa
authors.affiliation: Department of Microbiology and Immunology, Schulich School of Medicine and Dentistry, Western University, London, ON, Canada, N6A5C1.
authors.forename: Adrienne T
authors.initials: AT
authors.lastname: Wakabayashi
authors.affiliation: Department of Microbiology and Immunology, Schulich School of Medicine and Dentistry, Western University, London, ON, Canada, N6A5C1.
authors.forename: Katherine J
authors.initials: KJ
authors.lastname: Kasper
authors.affiliation: Department of Microbiology and Immunology, Schulich School of Medicine and Dentistry, Western University, London, ON, Canada, N6A5C1.
authors.forename: Stacey X
authors.initials: SX
authors.lastname: Xu
authors.affiliation: Department of Microbiology and Immunology, Schulich School of Medicine and Dentistry, Western University, London, ON, Canada, N6A5C1.|Lawson Health Research Institute, London, ON, Canada, N6A5C1.
authors.forename: S M Mansour
authors.initials: SMM
authors.lastname: Haeryfar
authors.affiliation: Department of Microbiology and Immunology, Schulich School of Medicine and Dentistry, Western University, London, ON, Canada, N6A5C1. john.mccormick@uwo.ca.|Lawson Health Research Institute, London, ON, Canada, N6A5C1. john.mccormick@uwo.ca.
authors.forename: John K
authors.initials: JK
authors.lastname: McCormick
mesh_terms: D000818:Animals; D058491:Bacterial Load; D004195:Disease Models, Animal; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology; D008213:Lymphocyte Activation; D051379:Mice; D009304:Nasopharyngitis / Q000276:immunology* / Q000382:microbiology*; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000378:metabolism; D013297:Streptococcus pyogenes / Q000276:immunology*; D018089:Superantigens / Q000276:immunology*; D037182:T-Cell Antigen Receptor Specificity / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism
chemical_list: D016693:Receptors, Antigen, T-Cell, alpha-beta; D018089:Superantigens
keywords: Group A Streptococcus; Nasopharyngeal infection; Streptococcus pyogenes; Superantigen; Transgenic mouse model; Vβ skewing