title: Mutagenic potential of fine wastes from dimension stone industry.
abstract: The industrial treatment of dimension stones, such as marbles and granites, includes a stage of plate polishing, in which resins and abrasives are used, producing a fine grained waste with high moisture content. These wastes pass through decantation tanks in order to separate the solid and liquid phases. Until now, there is no knowledge about the mutagenic effects that this effluent can cause to organisms exposed to it. Thus, this study evaluated the mutagenic potential of dimension stone polishing wastes in onion root cells and fish erythrocytes. The onion seeds were germinated in Petri dishes with filter paper moistened in the liquid phase of the effluent. After germination, the onion roots were prepared for analysis of chromosomal aberrations in meristematic cells. The fishes were exposed during 72h to the solid phase of the effluent diluted in pure groundwater. Blood samples were used for counting of micronucleus and nuclear abnormalities. The onion seeds had similar germination and mitotic index in all treatments. However, it was observed in the seeds exposed to the polishing waste, numbers significantly higher of micronucleus, nuclear buds and other chromosomal aberrations when compared with the negative control. The fishes exposed to the waste showed numbers significantly higher of micronucleus when compared with the negative control. The fishes from all treatments showed significant increase in nuclear abnormalities when compared to the negative control. We concluded that the analysed wastes have mutagenic potential at the studied conditions; this effect can be related to the high content of phenolic compounds identified in the samples.
authors.affiliation: Departamento de Biologia, Centro de Ciências Agrárias, Universidade Federal do Espírito Santo, Alegre, Espírito Santo CEP 29500-000, Brasil.
authors.forename: Luara Louzada
authors.initials: LL
authors.lastname: Aguiar
authors.affiliation: Departamento de Biologia, Centro de Ciências Agrárias, Universidade Federal do Espírito Santo, Alegre, Espírito Santo CEP 29500-000, Brasil.
authors.forename: Camila Bruschi
authors.initials: CB
authors.lastname: Tonon
authors.affiliation: Departamento de Biologia, Centro de Ciências Agrárias, Universidade Federal do Espírito Santo, Alegre, Espírito Santo CEP 29500-000, Brasil.
authors.forename: Erika Takagi
authors.initials: ET
authors.lastname: Nunes
authors.affiliation: Departamento de Biologia, Centro de Ciências Agrárias, Universidade Federal do Espírito Santo, Alegre, Espírito Santo CEP 29500-000, Brasil.
authors.forename: Adriane Cristina Araújo
authors.initials: AC
authors.lastname: Braga
authors.affiliation: Departamento de Geologia, Centro de Ciências Agrárias, Universidade Federal do Espírito Santo, Alegre, Espírito Santo CEP 29500-000, Brasil.
authors.forename: Mirna Aparecida
authors.initials: MA
authors.lastname: Neves
authors.affiliation: Departamento de Biologia, Centro de Ciências Agrárias, Universidade Federal do Espírito Santo, Alegre, Espírito Santo CEP 29500-000, Brasil. Electronic address: joseaugustodavid@hotmail.com.
authors.forename: José Augusto
authors.initials: JA
authors.lastname: de Oliveira David
mesh_terms: D000818:Animals; D002869:Chromosome Aberrations / Q000139:chemically induced; D032461:Chromosomes, Plant / Q000187:drug effects; D023681:Cichlids; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism; D018525:Germination / Q000187:drug effects; D007220:Industrial Waste / Q000032:analysis*; D066192:Manufacturing Industry; D018519:Meristem / Q000187:drug effects; D008940:Mitotic Index; D009153:Mutagens / Q000032:analysis* / Q000633:toxicity; D019697:Onions / Q000187:drug effects; D019800:Phenol / Q000032:analysis; D018517:Plant Roots / Q000187:drug effects; D012639:Seeds / Q000187:drug effects; D062065:Wastewater / Q000737:chemistry
chemical_list: D007220:Industrial Waste; D009153:Mutagens; D019800:Phenol; D062065:Wastewater
keywords: Allium cepa; Mutagenesis; Oreochromis niloticus; Phenolic compounds