title: Catalytic Molecular Imaging of MicroRNA in Living Cells by DNA-Programmed Nanoparticle Disassembly.
abstract: Molecular imaging is an essential tool for disease diagnostics and treatment. Direct imaging of low-abundance nucleic acids in living cells remains challenging because of the relatively low sensitivity and insufficient signal-to-background ratio of conventional molecular imaging probes. Herein, we report a class of DNA-templated gold nanoparticle (GNP)-quantum dot (QD) assembly-based probes for catalytic imaging of cancer-related microRNAs (miRNA) in living cells with signal amplification capacity. We show that a single miRNA molecule could catalyze the disassembly of multiple QDs with the GNP through a DNA-programmed thermodynamically driven entropy gain process, yielding significantly amplified QD photoluminescence (PL) for miRNA imaging. By combining the robust PL of QDs with the catalytic amplification strategy, three orders of magnitude improvement in detection sensitivity is achieved in comparison with non-catalytic imaging probe, which enables facile and accurate differentiation between cancer cells and normal cells by miRNA imaging in living cells.
authors.affiliation: State and Local Joint Engineering Laboratory for Novel Functional Polymeric Materials, The Key Lab of Health Chemistry and Molecular Diagnosis of Suzhou, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou, 215123, P.R. China.
authors.forename: Xuewen
authors.initials: X
authors.lastname: He
authors.affiliation: State and Local Joint Engineering Laboratory for Novel Functional Polymeric Materials, The Key Lab of Health Chemistry and Molecular Diagnosis of Suzhou, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou, 215123, P.R. China.
authors.forename: Tao
authors.initials: T
authors.lastname: Zeng
authors.affiliation: State and Local Joint Engineering Laboratory for Novel Functional Polymeric Materials, The Key Lab of Health Chemistry and Molecular Diagnosis of Suzhou, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou, 215123, P.R. China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Li
authors.affiliation: State and Local Joint Engineering Laboratory for Novel Functional Polymeric Materials, The Key Lab of Health Chemistry and Molecular Diagnosis of Suzhou, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou, 215123, P.R. China.
authors.forename: Ganglin
authors.initials: G
authors.lastname: Wang
authors.affiliation: State and Local Joint Engineering Laboratory for Novel Functional Polymeric Materials, The Key Lab of Health Chemistry and Molecular Diagnosis of Suzhou, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou, 215123, P.R. China. nan.ma@suda.edu.cn.
authors.forename: Nan
authors.initials: N
authors.lastname: Ma
mesh_terms: D002384:Catalysis; D045744:Cell Line, Tumor; D004247:DNA / Q000737:chemistry*; D004587:Electrophoresis, Agar Gel; D006801:Humans; D035683:MicroRNAs / Q000737:chemistry*; D053758:Nanoparticles; D045663:Quantum Dots
chemical_list: D035683:MicroRNAs; D004247:DNA
keywords: catalysis; gold nanoparticles; microRNAs; molecular imaging; quantum dots