title: In vivo depletion and genetic targeting of mouse intestinal CX3CR1(+) mononuclear phagocytes.
abstract: Mononuclear phagocytes (MPs) are an essential component of the intestinal immune system. They are comprised of a few dendritic cell and macrophage subsets, all with the common ability to sample extracellular milieu and to discriminate between dangerous and innocuous signals. Despite the commonality, each MP subset acquires distinct developmental pathways and unique functions, likely to fulfill needs of the tissue in which they reside. Some MP subsets develop from monocytes and are distinguished by their expression of CX3C-chemokine receptor 1 (CX3CR1). This manuscript summarizes our expertise in vivo targeting of intestinal CX3CR1(+) MP subsets. The described tools might be useful for studies of CX3CR1(+) MP function in various murine experimental models, particularly under non-inflammatory conditions.
authors.affiliation: Department of Microbiology and Immunology, Penn State University College of Medicine and Milton Hershey Medical Center, Hershey, PA 17033, USA.
authors.forename: Balázs
authors.initials: B
authors.lastname: Koscsó
authors.affiliation: Department of Microbiology and Immunology, Penn State University College of Medicine and Milton Hershey Medical Center, Hershey, PA 17033, USA.
authors.forename: Kavitha
authors.initials: K
authors.lastname: Gowda
authors.affiliation: Department of Microbiology and Immunology, Penn State University College of Medicine and Milton Hershey Medical Center, Hershey, PA 17033, USA. Electronic address: mbogunovich@hmc.psu.edu.
authors.forename: Milena
authors.initials: M
authors.lastname: Bogunovic
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D015415:Biomarkers / Q000378:metabolism; D000073883:CX3C Chemokine Receptor 1; D019070:Cell Lineage; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D015536:Down-Regulation; D018390:Gene Targeting / Q000379:methods*; D005838:Genotype; D006825:Hybridomas; D018928:Immunity, Mucosal; D016130:Immunophenotyping; D019426:Integrases / Q000235:genetics; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000187:drug effects / Q000276:immunology; D008264:Macrophages / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009113:Muramidase / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D010641:Phenotype; D011401:Promoter Regions, Genetic; D019707:Receptors, Chemokine / Q000172:deficiency* / Q000235:genetics; D016187:Receptors, Granulocyte-Macrophage Colony-Stimulating Factor / Q000037:antagonists & inhibitors / Q000235:genetics / Q000276:immunology / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D015415:Biomarkers; D000073883:CX3C Chemokine Receptor 1; C000604004:Csf1r protein, mouse; C471885:Cx3cr1 protein, mouse; D019707:Receptors, Chemokine; D016187:Receptors, Granulocyte-Macrophage Colony-Stimulating Factor; C045073:Cre recombinase; D019426:Integrases; D009113:Muramidase