title: Molecular characterisation of Mycoplasma hyorhinis isolated from pigs using pulsed-field gel electrophoresis and 16S rRNA sequencing.
abstract: Economic loss in pig breeding is common due to respiratory disorders, and Mycoplasma hyopneumoniae and Mycoplasma hyorhinis, namely, are the most common infectious agents. The aim of this study is to recover these mollicutes and detect their genotypic variations by pulsed-field gel electrophoresis (PFGE) and sequencing the 16 s rRNA gene. One hundred and twenty-six swabs from tonsil and nasal mucus of pigs with respiratory disorders were analysed. A total of 78 lungs were sampled, as well as two trachea and two tonsils obtained from animals with respiratory disorder. A total of 59 isolates were obtained: 1 (1.70 per cent) of M hyopneumoniae, 2 (3.40 per cent) of Mycoplasma flocculare and 56 (94.90 per cent) of M hyorhinis. The PFGE for M hyorhinis showed 10 profiles with enzyme AvaI and 9 profiles with XhoI. A low polymorphism of the 16sRNS gene was detected in M hyorhinis isolates compared with the type strain in the GenBank. M hyorhinis isolates of different herds showed a large heterogenicity with enzymes AvaI and XhoI. The sequencing of the 16S rRNA gene allowed for analysing the interspecific and intraspecific variations of isolated mycoplasmas.
authors.affiliation: Departamento de Microbiologia , Instituto de Ciências Biomédicas, Universidade de São Paulo , São Paulo, São Paulo , Brazil.
authors.forename: Maurício
authors.initials: M
authors.lastname: Yamaguti
authors.affiliation: Departamento de Microbiologia , Instituto de Ciências Biomédicas, Universidade de São Paulo , São Paulo, São Paulo , Brazil.
authors.forename: Rosângela C
authors.initials: RC
authors.lastname: Oliveira
authors.affiliation: Departamento de Microbiologia , Instituto de Ciências Biomédicas, Universidade de São Paulo , São Paulo, São Paulo , Brazil ; Instituto Multidisciplinar em Saúde, Núcleo de Tecnologia em Saúde, Universidade Federal da Bahia , Vitória da Conquista, Bahia , Brazil.
authors.forename: Lucas M
authors.initials: LM
authors.lastname: Marques
authors.affiliation: Departamento de Microbiologia , Instituto de Ciências Biomédicas, Universidade de São Paulo , São Paulo, São Paulo , Brazil.
authors.forename: Melissa
authors.initials: M
authors.lastname: Buzinhani
authors.affiliation: Departamento de Microbiologia , Instituto de Ciências Biomédicas, Universidade de São Paulo , São Paulo, São Paulo , Brazil.
authors.forename: Marcos R
authors.initials: MR
authors.lastname: Buim
authors.affiliation: Departamento de Microbiologia , Instituto de Ciências Biomédicas, Universidade de São Paulo , São Paulo, São Paulo , Brazil.
authors.forename: Renata L
authors.initials: RL
authors.lastname: Neto
authors.affiliation: Departamento de Microbiologia , Instituto de Ciências Biomédicas, Universidade de São Paulo , São Paulo, São Paulo , Brazil.
authors.forename: Ana Márcia S
authors.initials: AM
authors.lastname: Guimarães
authors.affiliation: Departamento de Microbiologia , Instituto de Ciências Biomédicas, Universidade de São Paulo , São Paulo, São Paulo , Brazil.
authors.forename: Jorge
authors.initials: J
authors.lastname: Timenetsky
keywords: Mycoplasmas; Pigs; Respiratory disease