title: Effect of three typical sulfide mineral flotation collectors on soil microbial activity.
abstract: The sulfide mineral flotation collectors are wildly used in China, whereas their toxic effect on soil microbial activity remains largely unexplored. In this study, isothermal microcalorimetric technique and soil enzyme assay techniques were employed to investigate the toxic effect of typical sulfide mineral flotation collectors on soil microbial activity. Soil samples were treated with different concentrations (0-100 μg•g - 1 soil) of butyl xanthate, butyl dithiophosphate, and sodium diethyldithiocarbamate. Results showed a significant adverse effect of butyl xanthate (p < 0.05), butyl dithiophosphate, and sodium diethyldithiocarbamate (p < 0.01) on soil microbial activity. The growth rate constants k decreased along with the increase of flotation collectors concentration from 20.0 to 100.0 μg•g(-1). However, the adverse effects of these three floatation collectors showed significant difference. The IC 20 of the investigated flotation reagents followed such an order: IC 20 (butyl xanthate) > IC 20 (sodium diethyldithiocarbamate) > IC 20 (butyl dithiophosphate) with their respective inhibitory concentration as 47.03, 38.36, and 33.34 μg•g(-1). Besides, soil enzyme activities revealed that these three flotation collectors had an obvious effect on fluorescein diacetate hydrolysis (FDA) enzyme and catalase (CAT) enzyme. The proposed methods can provide meaningful toxicological information of flotation reagents to soil microbes in the view of metabolism and biochemistry, which are consistent and correlated to each other.
authors.affiliation: School of Civil and Environmental Engineering, and National International Cooperation Base on Environment and Energy, University of Science and Technology Beijing, 30 Xueyuan Road, 100083, Beijing, People's Republic of China.
authors.forename: Zunwei
authors.initials: Z
authors.lastname: Guo
authors.affiliation: School of Civil and Environmental Engineering, and National International Cooperation Base on Environment and Energy, University of Science and Technology Beijing, 30 Xueyuan Road, 100083, Beijing, People's Republic of China. yaojun@ustb.edu.cn.
authors.forename: Jun
authors.initials: J
authors.lastname: Yao
authors.affiliation: School of Civil and Environmental Engineering, and National International Cooperation Base on Environment and Energy, University of Science and Technology Beijing, 30 Xueyuan Road, 100083, Beijing, People's Republic of China.
authors.forename: Fei
authors.initials: F
authors.lastname: Wang
authors.affiliation: School of Civil and Environmental Engineering, and National International Cooperation Base on Environment and Energy, University of Science and Technology Beijing, 30 Xueyuan Road, 100083, Beijing, People's Republic of China.
authors.forename: Zhimin
authors.initials: Z
authors.lastname: Yuan
authors.affiliation: School of Civil and Environmental Engineering, and National International Cooperation Base on Environment and Energy, University of Science and Technology Beijing, 30 Xueyuan Road, 100083, Beijing, People's Republic of China.
authors.forename: P
authors.initials: P
authors.lastname: Bararunyeretse
authors.affiliation: School of Civil and Environmental Engineering, and National International Cooperation Base on Environment and Energy, University of Science and Technology Beijing, 30 Xueyuan Road, 100083, Beijing, People's Republic of China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhao
mesh_terms: D002151:Calorimetry / Q000379:methods; D002374:Catalase / Q000378:metabolism; D002681:China; D004050:Ditiocarb / Q000633:toxicity*; D057075:Enzyme Assays; D008903:Minerals; D063086:Organothiophosphates / Q000633:toxicity*; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000633:toxicity*; D013440:Sulfides; D013871:Thiones / Q000633:toxicity*
chemical_list: D008903:Minerals; D063086:Organothiophosphates; D012987:Soil; D012989:Soil Pollutants; D013440:Sulfides; D013871:Thiones; C025099:butylxanthate; C062566:dibutyl dithiophosphate; D004050:Ditiocarb; D002374:Catalase
keywords: Butyl dithiophosphate; Butyl xanthate; Flotation collectors; Microbial activity; Microcalorimetry; Sodium diethyldithiocarbamate; Soil