title: Identification and characterization of antioxidant peptides obtained by gastrointestinal digestion of amaranth proteins.
abstract: The objective of the present work was to separate and identify antioxidant peptides from a simulated gastrointestinal digest (Id) from Amaranthus mantegazzianus proteins (I), which has previously been demonstrated to have this activity. I and Id were separated by preparative RP-HPLC. Fractions were evaluated by the ORAC method and the more active ones were analyzed by LC-MS/MS. Each fraction presented diverse peptides from different proteins, most of them from the 11S globulin. After grouping the peptides from 11S globulin according to their overlapping sequences, and based on previous information about structure-activity relationships, ten sequences were synthesized, in order to evaluate their antioxidant activity. Four peptides presented interesting activity: AWEEREQGSR>YLAGKPQQEH∼IYIEQGNGITGM∼TEVWDSNEQ. They exhibited some of the structural characteristics already known to demonstrate this activity, all of them containing at least one bulky aromatic residue. All belonged to little structured, internal or exposed regions of the acid subunit of the 11S globulin.
authors.affiliation: Centro de Investigación y Desarrollo en Criotecnología de Alimentos (CIDCA), UNLP-CONICET, 47 y 116, 1900 La Plata, Argentina. Electronic address: mceciliaorsini@gmail.com.
authors.forename: María C
authors.initials: MC
authors.lastname: Orsini Delgado
authors.affiliation: Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Roque Saenz Pena 182, B1876BXD Bernal, Argentina. Electronic address: agusnardo@hotmail.com.
authors.forename: Agustina
authors.initials: A
authors.lastname: Nardo
authors.affiliation: UR1268 Biopolymères Interactions Assemblages, INRA, F-44300 Nantes, France. Electronic address: marija.pavlovic@nantes.inra.fr.
authors.forename: Marija
authors.initials: M
authors.lastname: Pavlovic
authors.affiliation: UR1268 Biopolymères Interactions Assemblages, INRA, F-44300 Nantes, France. Electronic address: Helene.Rogniaux@nantes.inra.fr.
authors.forename: Hélène
authors.initials: H
authors.lastname: Rogniaux
authors.affiliation: Centro de Investigación y Desarrollo en Criotecnología de Alimentos (CIDCA), UNLP-CONICET, 47 y 116, 1900 La Plata, Argentina. Electronic address: mcacidca@gmail.com.
authors.forename: María C
authors.initials: MC
authors.lastname: Añón
authors.affiliation: Centro de Investigación y Desarrollo en Criotecnología de Alimentos (CIDCA), UNLP-CONICET, 47 y 116, 1900 La Plata, Argentina. Electronic address: vtironi@quimica.unlp.edu.ar.
authors.forename: Valeria A
authors.initials: VA
authors.lastname: Tironi
mesh_terms: D027721:Amaranthus / Q000737:chemistry*; D000975:Antioxidants / Q000032:analysis* / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D004063:Digestion; D041981:Gastrointestinal Tract / Q000378:metabolism; D010455:Peptides / Q000032:analysis* / Q000494:pharmacology; D010940:Plant Proteins / Q000032:analysis* / Q000494:pharmacology; D013329:Structure-Activity Relationship; D053719:Tandem Mass Spectrometry
chemical_list: D000975:Antioxidants; D010455:Peptides; D010940:Plant Proteins
keywords: Amaranth proteins; Antioxidant activity; Gastrointestinal digestion; Peptides; Structure–activity relationship