title: Proteomic analysis of salt-responsive proteins in oat roots (Avena sativa L.).
abstract: BACKGROUND
  Oat is considered as a moderately salt-tolerant crop that could be used to improve saline and alkaline soil. Previous studies have focused on short-term salt stress exposure (0.5-48 h), while molecular mechanisms of salt tolerance in oat remain unclear.
  
  
  RESULTS
  Long-term salt stress (16 days) increased the levels of superoxide dismutase activity, peroxidase activity, malondialdehyde content, putrescine content, spermidine content and soluble sugar content and reduced catalase activity in oat roots. The stress also caused changes in protein profiles in the roots. At least 1400 reproducible protein spots were identified in a two-dimensional electrophoresis gel, among which 23 were differentially expressed between treated vs control plants and 13 were identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
  
  
  CONCLUSIONS
  These differentially expressed proteins are involved in five types of biological process: (1) two fructose-bisphosphate aldolases, four alcohol dehydrogenases, an enolase, a UDP-glucuronic acid decarboxylase and an F1-ATPase alpha subunit related to carbohydrate and energy metabolism; (2) a choline monooxygenase related to stress and defense; (3) a lipase related to fat metabolism; (4) a polyubiquitin related to protein degradation; (5) a 14-3-3 protein related to signaling. © 2015 Society of Chemical Industry.
authors.affiliation: Science Innovation Team of Oats, Inner Mongolia Agricultural University, 010019 Hohhot, Inner Mongolia, China.
authors.forename: Jianhui
authors.initials: J
authors.lastname: Bai
authors.affiliation: Science Innovation Team of Oats, Inner Mongolia Agricultural University, 010019 Hohhot, Inner Mongolia, China.
authors.forename: Jinghui
authors.initials: J
authors.lastname: Liu
authors.affiliation: Science Innovation Team of Oats, Inner Mongolia Agricultural University, 010019 Hohhot, Inner Mongolia, China.
authors.forename: Weihong
authors.initials: W
authors.lastname: Jiao
authors.affiliation: Science Innovation Team of Oats, Inner Mongolia Agricultural University, 010019 Hohhot, Inner Mongolia, China.
authors.forename: Rula
authors.initials: R
authors.lastname: Sa
authors.affiliation: Science Innovation Team of Oats, Inner Mongolia Agricultural University, 010019 Hohhot, Inner Mongolia, China.
authors.forename: Na
authors.initials: N
authors.lastname: Zhang
authors.affiliation: Hawaii Agriculture Research Center, Kunia, HI, 96759 USA.
authors.forename: Ruizong
authors.initials: R
authors.lastname: Jia
mesh_terms: D018554:Avena / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D018384:Oxidative Stress; D009195:Peroxidase / Q000235:genetics / Q000378:metabolism; D018515:Plant Leaves / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D011700:Putrescine / Q000378:metabolism; D054712:Salinity; D055051:Salt-Tolerant Plants / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013312:Stress, Physiological; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D053719:Tandem Mass Spectrometry; D057825:Two-Dimensional Difference Gel Electrophoresis
chemical_list: D010940:Plant Proteins; D009195:Peroxidase; D013482:Superoxide Dismutase; D011700:Putrescine
keywords: oat; physiological indices; proteomic analysis; salt stress