title: MicroRNA-381 Negatively Regulates TLR4 Signaling in A549 Cells in Response to LPS Stimulation.
abstract: It is widely reported that miR-381 is dysregulated in various tumors. However, the specific role of miR-381 in respiratory infections has not been reported. To probe this role, A549 cells were pretreated with 1 μg/mL LPS for 24 h. The level of miR-381 was detected using RT-qPCR. The expression of proinflammatory cytokines was determined using an ELISA kit and western blotting. Bioinformatics analysis was used to predict the target genes of miR-381, and a luciferase reporter assay was used to validate the expression of the target genes. miR-381 expression was increased in A549 cells treated with LPS, which is a ligand of TLRs. Further study revealed that the overexpression of miR-381 increased the activity of NF-κB signaling, thereby increasing the expression of IL-6, TNFα, and COX-2. Further study revealed that IκBα was a target gene of miR-381. The upregulation of miR-381 under LPS stimulation contributes to respiratory infections mainly by targeting IκBα.
authors.affiliation: The Fourth Affiliated Hospital of Zhejiang University School of Medicine, No. 1 City Mall Street, Yiwu, Zhejiang 322000, China.
authors.forename: Zhihao
authors.initials: Z
authors.lastname: Xu
authors.affiliation: The Fourth Affiliated Hospital of Zhejiang University School of Medicine, No. 1 City Mall Street, Yiwu, Zhejiang 322000, China.
authors.forename: Dapeng
authors.initials: D
authors.lastname: Dong
authors.affiliation: The Fourth Affiliated Hospital of Zhejiang University School of Medicine, No. 1 City Mall Street, Yiwu, Zhejiang 322000, China.
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Chen
authors.affiliation: The Second Affiliated Hospital of Zhejiang University School of Medicine, Jiefang Road 88, Hangzhou, Zhejiang 310009, China.
authors.forename: Huaqiong
authors.initials: H
authors.lastname: Huang
authors.affiliation: The Fourth Affiliated Hospital of Zhejiang University School of Medicine, No. 1 City Mall Street, Yiwu, Zhejiang 322000, China.
authors.forename: Shenglan
authors.initials: S
authors.lastname: Wen
mesh_terms: D002461:Cell Line, Transformed; D051546:Cyclooxygenase 2 / Q000096:biosynthesis / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis / Q000235:genetics; D008070:Lipopolysaccharides / Q000633:toxicity*; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects*; D051197:Toll-Like Receptor 4 / Q000819:agonists* / Q000235:genetics / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000235:genetics
chemical_list: C508600:IL6 protein, human; D015850:Interleukin-6; D008070:Lipopolysaccharides; C588249:MIRN381 microRNA, human; D035683:MicroRNAs; D016328:NF-kappa B; C495345:TLR4 protein, human; D051197:Toll-Like Receptor 4; D014409:Tumor Necrosis Factor-alpha; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human