title: A Role for Arabidopsis miR399f in Salt, Drought, and ABA Signaling.
abstract: MiR399f plays a crucial role in maintaining phosphate homeostasis in Arabidopsis thaliana. Under phosphate starvation conditions, AtMYB2, which plays a role in plant salt and drought stress responses, directly regulates the expression of miR399f. In this study, we found that miR399f also participates in plant responses to abscisic acid (ABA), and to abiotic stresses including salt and drought. Salt and ABA treatment induced the expression of miR399f, as confirmed by histochemical analysis of promoter-GUS fusions. Transgenic Arabidopsis plants overexpressing miR399f (miR399f-OE) exhibited enhanced tolerance to salt stress and exogenous ABA, but hypersensitivity to drought. Our in silico analysis identified ABF3 and CSP41b as putative target genes of miR399f, and expression analysis revealed that mRNA levels of ABF3 and CSP41b decreased remarkably in miR399f-OE plants under salt stress and in response to treatment with ABA. Moreover, we showed that activation of stress-responsive gene expression in response to salt stress and ABA treatment was impaired in miR399f-OE plants. Thus, these results suggested that in addition to phosphate starvation signaling, miR399f might also modulates plant responses to salt, ABA, and drought, by regulating the expression of newly discovered target genes such as ABF3 and CSP41b.
authors.affiliation: Division of Applied Life Science (BK21 Plus Program), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju 660-701, Korea.
authors.forename: Dongwon
authors.initials: D
authors.lastname: Baek
authors.affiliation: Division of Applied Life Science (BK21 Plus Program), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju 660-701, Korea.
authors.forename: Hyun Jin
authors.initials: HJ
authors.lastname: Chun
authors.affiliation: Division of Applied Life Science (BK21 Plus Program), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju 660-701, Korea.
authors.forename: Songhwa
authors.initials: S
authors.lastname: Kang
authors.affiliation: Division of Applied Life Science (BK21 Plus Program), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju 660-701, Korea.
authors.forename: Gilok
authors.initials: G
authors.lastname: Shin
authors.affiliation: Division of Applied Life Science (BK21 Plus Program), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju 660-701, Korea.
authors.forename: Su Jung
authors.initials: SJ
authors.lastname: Park
authors.affiliation: Division of Applied Life Science (BK21 Plus Program), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju 660-701, Korea.
authors.forename: Hyewon
authors.initials: H
authors.lastname: Hong
authors.affiliation: Division of Applied Life Science (BK21 Plus Program), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju 660-701, Korea.
authors.forename: Chanmin
authors.initials: C
authors.lastname: Kim
authors.affiliation: College of Life Science and Natural Resources, Dong-A University, Busan 604-714, Korea.
authors.forename: Doh Hoon
authors.initials: DH
authors.lastname: Kim
authors.affiliation: Division of Applied Life Science (BK21 Plus Program), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju 660-701, Korea.
authors.forename: Sang Yeol
authors.initials: SY
authors.lastname: Lee
authors.affiliation: Division of Applied Life Science (BK21 Plus Program), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju 660-701, Korea.
authors.forename: Min Chul
authors.initials: MC
authors.lastname: Kim
authors.affiliation: Division of Applied Life Science (BK21 Plus Program), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju 660-701, Korea.
authors.forename: Dae-Jin
authors.initials: DJ
authors.lastname: Yun
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism / Q000494:pharmacology*; D000222:Adaptation, Physiological; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D050976:Basic-Leucine Zipper Transcription Factors / Q000235:genetics* / Q000378:metabolism; D055864:Droughts; D018506:Gene Expression Regulation, Plant; D017930:Genes, Reporter; D005916:Globulins / Q000235:genetics* / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D010710:Phosphates / Q000172:deficiency; D030821:Plants, Genetically Modified; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D054712:Salinity; D015398:Signal Transduction; D012965:Sodium Chloride / Q000494:pharmacology; D013312:Stress, Physiological; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism; D001617:beta-Glucosidase / Q000235:genetics / Q000378:metabolism
chemical_list: C506773:ABF3 protein, Arabidopsis; D029681:Arabidopsis Proteins; D050976:Basic-Leucine Zipper Transcription Factors; D005916:Globulins; C000601386:MIRN399 microRNA, Arabidopsis; C104175:MYB2 protein, Arabidopsis; D035683:MicroRNAs; D010710:Phosphates; D011993:Recombinant Fusion Proteins; D015534:Trans-Activators; C506890:cruciferin B, Arabidopsis; D012965:Sodium Chloride; D000040:Abscisic Acid; D001617:beta-Glucosidase
keywords: ABA; abiotic stress; arabidopsis; drought; microRNA; salt