title: Multilevel fluidic flow control in a rotationally-driven polyester film microdevice created using laser print, cut and laminate.
abstract: This paper presents a simple and cost-effective polyester toner microchip fabricated with laser print and cut lithography (PCL) to use with a battery-powered centrifugal platform for fluid handling. The combination of the PCL microfluidic disc and centrifugal platform: (1) allows parallel aliquoting of two different reagents of four different volumes ranging from nL to μL with an accuracy comparable to a piston-driven air pipette; (2) incorporates a reciprocating mixing unit driven by a surface-tension pump for further dilution of reagents, and (3) is amenable to larger scale integration of assay multiplexing (including all valves and mixers) without substantially increasing fabrication cost and time. For a proof of principle, a 10 min colorimetric assay for the quantitation of the protein level in the human blood plasma samples is demonstrated on chip with a limit of detection of ∼5 mg mL(-1) and coefficient of variance of ∼7%.
authors.affiliation: Department of Chemistry, University of Virginia, McCormick Road, Charlottesville, VA 22904, USA. jpl5e@virginia.edu.
authors.forename: Yiwen
authors.initials: Y
authors.lastname: Ouyang
authors.affiliation: Department of Chemistry, University of Virginia, McCormick Road, Charlottesville, VA 22904, USA. jpl5e@virginia.edu.
authors.forename: Jingyi
authors.initials: J
authors.lastname: Li
authors.affiliation: Mechanical Engineering, Georgia Institute of Technology, 315 Ferst Drive, Atlanta, GA 30332-0363, USA.
authors.forename: Christopher
authors.initials: C
authors.lastname: Phaneuf
authors.affiliation: Department of Chemistry, University of Virginia, McCormick Road, Charlottesville, VA 22904, USA. jpl5e@virginia.edu.
authors.forename: Paul S
authors.initials: PS
authors.lastname: Riehl
authors.affiliation: Mechanical Engineering, Georgia Institute of Technology, 315 Ferst Drive, Atlanta, GA 30332-0363, USA.
authors.forename: Craig
authors.initials: C
authors.lastname: Forest
authors.affiliation: Department of Mechanical Engineering & Materials Department, University of California Santa Barbara, Santa Barbara, CA 93106, USA.
authors.forename: Matthew
authors.initials: M
authors.lastname: Begley
authors.affiliation: Department of Pathology, University of Virginia Health Science Center, Charlottesville, VA 22908, USA.
authors.forename: Doris M
authors.initials: DM
authors.lastname: Haverstick
authors.affiliation: Department of Chemistry, University of Virginia, McCormick Road, Charlottesville, VA 22904, USA. jpl5e@virginia.edu and Department of Pathology, University of Virginia Health Science Center, Charlottesville, VA 22908, USA.
authors.forename: James P
authors.initials: JP
authors.lastname: Landers
mesh_terms: D002498:Centrifugation / Q000191:economics / Q000295:instrumentation; D003124:Colorimetry / Q000191:economics; D006801:Humans; D007834:Lasers; D046210:Microfluidic Analytical Techniques / Q000191:economics / Q000295:instrumentation; D011091:Polyesters / Q000737:chemistry*; D011327:Printing / Q000191:economics; D012399:Rotation; D012709:Serum Albumin / Q000032:analysis; D013500:Surface Tension
chemical_list: D011091:Polyesters; D012709:Serum Albumin