title: Native Australian plant extracts differentially induce Collagen I and Collagen III in vitro and could be important targets for the development of new wound healing therapies.
abstract: Australian native plants have a long history of therapeutic use in indigenous cultures, however, they have been poorly studied scientifically. We analysed the effects of 14 plant derived compounds from the species Pilidiostigma glabrum, Myoporum montanum, Geijera parviflora, and Rhodomyrtus psidioides for their potential wound healing properties by assessing their ability to induce or suppress Collagen I and Collagen III expression in human skin fibroblasts in culture. The compound 7-geranyloxycoumarin was able to significantly increase Collagen I (23.7%, p<0.0002) expression in comparison to control. Significant suppression of Collagen III was observed for the compounds flindersine (11.1%, p<0.02), and (N-acetoxymethyl) flindersine (27%, p<0.00005). The implications of these finding is that these compounds could potentially alter the expression of different collagens in the skin allowing for the potential development of new wound healing therapies and new approaches for treating various skin diseases as well as photo (sun) damaged, and aged skin.
authors.affiliation: Regenerative Medicine, Future Industries Institute, University of South Australia, Mawson Lakes, South Australia 5095, Australia.
authors.forename: Damian H
authors.initials: DH
authors.lastname: Adams
authors.affiliation: Southern Cross Plant Science, Southern Cross University, Lismore, New South Wales 2480, Australia.
authors.forename: Qingyao
authors.initials: Q
authors.lastname: Shou
authors.affiliation: Southern Cross Plant Science, Southern Cross University, Lismore, New South Wales 2480, Australia; Integria Healthcare, Gallans Rd., Ballina, New South Wales 2478, Australia.
authors.forename: Hans
authors.initials: H
authors.lastname: Wohlmuth
authors.affiliation: Regenerative Medicine, Future Industries Institute, University of South Australia, Mawson Lakes, South Australia 5095, Australia. Electronic address: allison.cowin@unisa.edu.au.
authors.forename: Allison J
authors.initials: AJ
authors.lastname: Cowin
mesh_terms: D001315:Australia; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis*; D003374:Coumarins / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D015394:Molecular Structure; D027822:Myrtaceae / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*; D027821:Rutaceae / Q000737:chemistry*; D028083:Scrophulariaceae / Q000737:chemistry*; D012867:Skin / Q000166:cytology; D014945:Wound Healing / Q000187:drug effects
chemical_list: D003374:Coumarins; D010936:Plant Extracts; D003094:Collagen; C105832:aurapten
keywords: Australian native plants; Collagen; Fibroblast; Fluorescence; Modulation