title: Correlation between expressions of Cyclin-D1, EGFR and p53 with chemoradiation response in patients of locally advanced oral squamous cell carcinoma.
abstract: BACKGROUND
  Cyclin-D1, p53 and EGFR are molecular markers that regulate the cell cycle and play an important role in tumor progression and development. The present study evaluates the prognostic significance of these markers with chemoradiation response in patients of locally advanced oral squamous cell carcinoma (OSCC).
  
  
  METHODS
  A total of 97 OSCC patients (females = 19 and males = 78), aged 20-67 years and stage III/IV were recruited. Treatment response was assessed according to WHO criteria. Cyclin-D1, p53 and EGFR expressions in tumor tissue was estimated by immunohistochemical (IHC) method and quantified as percentage positive nuclei.
  
  
  RESULTS
  The positive expression rates of molecular markers were 86.6% for Cyclin-D1, 92.8% for EGFR and 85.6% for p53. The strong positive expressions of both Cyclin-D1 and p53 showed significant association with poor response. The Cox multivariate regression analysis showed coexpressions of Cyclin-D1 and p53 a significant and independent predictor of overall survival (OR = 1.90, 95% CI = 1.45-4.82, p = 0.046) after adjusting the demographic, clinicopathological and radiological response. The strong positive expressions of Cyclin-D1 and p53 and coexpressions of Cyclin-D1, EGFR and p53 showed significant (p < 0.05 or p < 0.01 or p < 0.001) and lower survival as compared to negative or moderate positive expressions and coexpressions, respectively.
  
  
  CONCLUSIONS
  Expressions and coexpressions of Cyclin-D1 and p53 may serve as a prognostic marker in OSCC patients.
authors.affiliation: Department of Radiotherapy, King George's Medical University, Lucknow, Uttar Pradesh, India.
authors.forename: Huma
authors.initials: H
authors.lastname: Khan
authors.affiliation: Department of Radiotherapy, King George's Medical University, Lucknow, Uttar Pradesh, India.
authors.forename: Seema
authors.initials: S
authors.lastname: Gupta
authors.affiliation: Department of Pathology, RMLIMS, Lucknow, Uttar Pradesh, India.
authors.forename: Nuzhat
authors.initials: N
authors.lastname: Husain
authors.affiliation: Department of Surgical Oncology, King George's Medical University, Lucknow, Uttar Pradesh, India.
authors.forename: Sanjeev
authors.initials: S
authors.lastname: Misra
authors.affiliation: Clinical and Experimental Medicine Division, CSIR-Central Drug Research Institute, Lucknow, Uttar Pradesh, India.
authors.forename: Negi
authors.initials: N
authors.lastname: Mps
authors.affiliation: Department of Radiotherapy, King George's Medical University, Lucknow, Uttar Pradesh, India.
authors.forename: Naseem
authors.initials: N
authors.lastname: Jamal
authors.affiliation: Clinical and Experimental Medicine Division, CSIR-Central Drug Research Institute, Lucknow, Uttar Pradesh, India.
authors.forename: Ashim
authors.initials: A
authors.lastname: Ghatak
keywords: Chemoradiation; Cyclin-D1; EGFR; Squamous cell carcinoma; p53 Immunohistochemistry