title: Anti-obesity Effect of Dioscorea oppositifolia Extract in High-Fat Diet-Induced Obese Mice and Its Chemical Characterization.
abstract: Dioscorea oppositifolia is a well-known edible and traditional medicine for the treatment of gastrointestinal diseases. In our previous study, D. oppositifolia exhibited both pancreatic lipase inhibition and an anti-adipogenesis effect in vitro. This study was performed to investigate the anti-obesity effect of D. oppositifolia on high-fat diet-induced obese mice. Female ICR mice were fed a high-fat diet with the 100 mg/kg of D. oppositifolia n-BuOH extract for 8 weeks. The high-fat diet mice received the 15 mg/kg Orlistat orally as a positive control. The body weight, parametrial adipose tissue weight, and the levels of triglyceride (TG), total cholesterol (TC), and low density lipoprotein (LDL)-cholesterol in blood serum of female ICR mice were significantly decreased by feeding a high-fat diet with the n-BuOH extract of D. oppositifolia. An inhibitory effect of D. oppositifolia extract on dietary fat absorption was also clearly shown. The D. oppositifolia sample was found to contain 3,5-dimethoxy-2,7-phenanthrenediol and (3R,5R)-3,5-dihydroxy-1,7-bis(4-hydroxyphenyl)-3,5-heptanediol as main components based on its phytochemical analysis. The present study is the first report of the anti-obesity effect by D. oppositifolia n-BuOH extract using an established disease model. The increase in fecal fat excretion by treatment of D. oppositifolia may be an effective approach for treating obesity and related diseases.
authors.affiliation: Department of Agronomy & Medicinal Plant Resources, College of Life Sciences and Natural Resources, Gyeongnam National University of Science and Technology.
authors.forename: Eun Ju
authors.initials: EJ
authors.lastname: Jeong
authors.forename: Jonghwan
authors.initials: J
authors.lastname: Jegal
authors.forename: Jongmin
authors.initials: J
authors.lastname: Ahn
authors.forename: Jinwoong
authors.initials: J
authors.lastname: Kim
authors.forename: Min Hye
authors.initials: MH
authors.lastname: Yang
mesh_terms: D020001:1-Butanol / Q000737:chemistry; D000273:Adipose Tissue / Q000187:drug effects / Q000254:growth & development; D000818:Animals; D019440:Anti-Obesity Agents / Q000494:pharmacology / Q000627:therapeutic use*; D002784:Cholesterol / Q000097:blood; D059305:Diet, High-Fat; D027742:Dioscorea; D005243:Feces / Q000737:chemistry; D005260:Female; D050356:Lipid Metabolism / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D008813:Mice, Inbred ICR; D009765:Obesity / Q000097:blood / Q000188:drug therapy*; D009929:Organ Size / Q000187:drug effects; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D027343:Rhizome / Q000737:chemistry; D014280:Triglycerides / Q000097:blood
chemical_list: D019440:Anti-Obesity Agents; D010936:Plant Extracts; D014280:Triglycerides; D020001:1-Butanol; D002784:Cholesterol