title: Duration of hexobarbital-induced sleep and monoamine oxidase activities in rat brain: Focus on the behavioral activity and on the free-radical oxidation.
abstract: The present study is focused on the relationship between monoamine oxidase (MAO) activity and hepatic content of cytochrome P450 (CYP), which reflects the status of microsomal oxidation. For vital integrative evaluation of hepatic microsomal oxidation in rats, the hexobarbital sleep test was used, and content of CYP was measured in hepatic microsomes. Rats with short hexobarbital sleep time (SHST) had higher content of microsomal CYP than rats with long hexobarbital sleep time (LHST). Whole brain MAO-A and MAO-B activities, serotonin and carbonylated protein levels were higher in SHST than in LHST rats. MAO-A and MAO-B activities were higher in brain cortex of SHST rats; MAO-A activity was higher only in hypothalamus and medulla of LHST. The same brain regions of LHST rats had higher concentrations of carbonylated proteins and lipid peroxidation products than in SHST rats. MAO activity was correlated with microsomal oxidation phenotype. Rats with higher hepatic content of CYP had higher activities of MAO-A and MAO-B in the brain and higher plasma serotonin levels than rats with lower microsomal oxidation. In conclusion, data obtained in this study showed a correlation between MAO activity and microsomal oxidation phenotype.
authors.affiliation: South-Ural State Medical University, Chelyabinsk, Russia. vadimed@yandex.ru.
authors.forename: Vadim E
authors.initials: VE
authors.lastname: Tseilikman
authors.forename: Denis A
authors.initials: DA
authors.lastname: Kozochkin
authors.forename: Eugenia B
authors.initials: EB
authors.lastname: Manukhina
authors.forename: H Fred
authors.initials: HF
authors.lastname: Downey
authors.forename: Olga B
authors.initials: OB
authors.lastname: Tseilikman
authors.forename: Maria E
authors.initials: ME
authors.lastname: Misharina
authors.forename: Anna A
authors.initials: AA
authors.lastname: Nikitina
authors.forename: Maria V
authors.initials: MV
authors.lastname: Komelkova
authors.forename: Maxim S
authors.initials: MS
authors.lastname: Lapshin
authors.forename: Marina V
authors.initials: MV
authors.lastname: Kondashevskaya
authors.forename: Svetlana S
authors.initials: SS
authors.lastname: Lazuko
authors.forename: Oxana V
authors.initials: OV
authors.lastname: Kusina
authors.forename: Marat V
authors.initials: MV
authors.lastname: Sahabutdinov
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects / Q000502:physiology*; D001921:Brain / Q000187:drug effects / Q000201:enzymology*; D004789:Enzyme Activation / Q000187:drug effects; D005609:Free Radicals / Q000378:metabolism; D006591:Hexobarbital / Q000008:administration & dosage*; D006993:Hypnotics and Sedatives / Q000008:administration & dosage; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism; D008995:Monoamine Oxidase / Q000378:metabolism*; D010084:Oxidation-Reduction / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D012890:Sleep / Q000187:drug effects / Q000502:physiology*
chemical_list: D005609:Free Radicals; D006993:Hypnotics and Sedatives; D006591:Hexobarbital; D008995:Monoamine Oxidase