title: Search for the Function of NWC, Third Gene Within RAG Locus: Generation and Characterization of NWC-Deficient Mice.
abstract: NWC is a third gene within recombination activating gene (RAG) locus, which unlike RAG genes is ubiquitously expressed and encodes a unique protein containing three strongly evolutionarily conserved domains not found in any other known protein. To get insight into its function we identified several proteins co-immunoprecipitating with NWC protein and generated new NWC-deficient mice. Here, we present evidence that unlike many other ubiquitously expressed evolutionarily conserved proteins, functional inactivation of NWC does not cause any gross developmental, physiological or reproductive abnormalities and that under physiological conditions NWC may be involved in assembling and functioning of cilia, cell surface organelles found on nearly every eukaryotic cell.
authors.affiliation: Laboratory of Molecular and Cellular Immunology, Department of Tumor Immunology, L. Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland.
authors.forename: Monika
authors.initials: M
authors.lastname: Kasztura
authors.affiliation: Laboratory of Molecular and Cellular Immunology, Department of Tumor Immunology, L. Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland.
authors.forename: Lukasz
authors.initials: L
authors.lastname: Sniezewski
authors.affiliation: Laboratory of Molecular and Cellular Immunology, Department of Tumor Immunology, L. Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Laszkiewicz
authors.affiliation: Laboratory of Molecular and Cellular Immunology, Department of Tumor Immunology, L. Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland.
authors.forename: Michal
authors.initials: M
authors.lastname: Majkowski
authors.affiliation: Laboratory of Molecular and Cellular Immunology, Department of Tumor Immunology, L. Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland.
authors.forename: Kamil
authors.initials: K
authors.lastname: Kobak
authors.affiliation: Laboratory of Molecular and Cellular Immunology, Department of Tumor Immunology, L. Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland.
authors.forename: Karolina
authors.initials: K
authors.lastname: Peczek
authors.affiliation: Laboratory of Molecular and Cellular Immunology, Department of Tumor Immunology, L. Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland.
authors.forename: Sylwia
authors.initials: S
authors.lastname: Janik
authors.affiliation: Chair of Biostructure and Physiology, Department of Histology and Embryology, Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland.
authors.forename: Violetta
authors.initials: V
authors.lastname: Kapusniak
authors.affiliation: Laboratory of Tumor Immunology, Department of Tumor Immunology, L. Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland.
authors.forename: Arkadiusz
authors.initials: A
authors.lastname: Miazek
authors.affiliation: Laboratory of Molecular and Cellular Immunology, Department of Tumor Immunology, L. Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland. cebrat@iitd.pan.wroc.pl.
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Cebrat
authors.affiliation: Laboratory of Molecular and Cellular Immunology, Department of Tumor Immunology, L. Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland. kisielow@iitd.pan.wroc.pl.|Laboratory of Tumor Immunology, Department of Tumor Immunology, L. Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland. kisielow@iitd.pan.wroc.pl.
authors.forename: Pawel
authors.initials: P
authors.lastname: Kisielow
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D005434:Flow Cytometry; D005786:Gene Expression Regulation; D016654:Genes, RAG-1; D005838:Genotype; D057809:HEK293 Cells; D006801:Humans; D007150:Immunohistochemistry; D047468:Immunoprecipitation; D051379:Mice; D018345:Mice, Knockout; D008957:Models, Genetic; D041681:NIH 3T3 Cells; D010641:Phenotype; D053719:Tandem Mass Spectrometry
chemical_list: D004268:DNA-Binding Proteins
keywords: Cilia; Evolutionarily conserved genes; NWC protein function/partners; NWC-knockout mice; Recombination activating gene (RAG) locus