title: Elicitation of Diosgenin Production in Trigonella foenum-graecum (Fenugreek) Seedlings by Methyl Jasmonate.
abstract: The effects of methyl jasmonate (MeJA), an elicitor of plant defense mechanisms, on the biosynthesis of diosgenin, a steroidal saponin, were investigated in six fenugreek (Trigonella foenum-graecum) varieties (Gujarat Methi-2, Kasuri-1, Kasuri-2, Pusa Early Branching, Rajasthan Methi and Maharashtra Methi-5). Treatment with 0.01% MeJA increased diosgenin levels, in 12 days old seedlings, from 0.5%-0.9% to 1.1%-1.8%. In addition, MeJA upregulated the expression of two pivotal genes of the mevalonate pathway, the metabolic route leading to diosgenin: 3-hydroxy-3-methylglutaryl-CoA reductase (HMG) and sterol-3-β-glucosyl transferase (STRL). In particular, MeJA increased the expression of HMG and STRL genes by 3.2- and 22.2-fold, respectively, in the Gujarat Methi-2 variety, and by 25.4- and 28.4-fold, respectively, in the Kasuri-2 variety. Therefore, MeJA may be considered a promising elicitor for diosgenin production by fenugreek plants.
authors.affiliation: Department of Medical Genetics, Xcelris Labs Limited, Old Premchandnagar Road, Opp. Satyagrah Chhavani, Bodakdev, Ahmedabad-380015, Gujarat, India. spandan.chaudhary@gmail.com.
authors.forename: Spandan
authors.initials: S
authors.lastname: Chaudhary
authors.affiliation: Department of Medical Genetics, Xcelris Labs Limited, Old Premchandnagar Road, Opp. Satyagrah Chhavani, Bodakdev, Ahmedabad-380015, Gujarat, India. surendrakchikara@gmail.com.
authors.forename: Surendra K
authors.initials: SK
authors.lastname: Chikara
authors.affiliation: Department of Biotechnology, Kadi Sarva Vishwavidyalaya, II Floor, KBIPER Building, Kadi Campus, Sector-23, Gandhinagar-382023, Gujarat, India. mc_sharma2@rediffmail.com.
authors.forename: Mahesh C
authors.initials: MC
authors.lastname: Sharma
authors.affiliation: Department of Medical Genetics, Xcelris Labs Limited, Old Premchandnagar Road, Opp. Satyagrah Chhavani, Bodakdev, Ahmedabad-380015, Gujarat, India. abhinavchaudhary26@yahoo.co.in.
authors.forename: Abhinav
authors.initials: A
authors.lastname: Chaudhary
authors.affiliation: Department of Biotechnology, Hemchandracharya North Gujarat University, Patan-384265, Gujarat, India. samisyed40@gmail.com.
authors.forename: Bakhtiyar
authors.initials: B
authors.lastname: Alam Syed
authors.affiliation: Department of Medical Genetics, Xcelris Labs Limited, Old Premchandnagar Road, Opp. Satyagrah Chhavani, Bodakdev, Ahmedabad-380015, Gujarat, India. pooja.chaudhary@xcelrislabs.com.
authors.forename: Pooja S
authors.initials: PS
authors.lastname: Chaudhary
authors.affiliation: Department of Medical Genetics, Xcelris Labs Limited, Old Premchandnagar Road, Opp. Satyagrah Chhavani, Bodakdev, Ahmedabad-380015, Gujarat, India. aditya.mehta@xcelrislabs.com.
authors.forename: Aditya
authors.initials: A
authors.lastname: Mehta
authors.affiliation: Department of Medical Genetics, Xcelris Labs Limited, Old Premchandnagar Road, Opp. Satyagrah Chhavani, Bodakdev, Ahmedabad-380015, Gujarat, India. maulik.patel@xcelrislabs.com.
authors.forename: Maulik
authors.initials: M
authors.lastname: Patel
authors.affiliation: Department of Medical Genetics, Xcelris Labs Limited, Old Premchandnagar Road, Opp. Satyagrah Chhavani, Bodakdev, Ahmedabad-380015, Gujarat, India. arpita.ghosh@xcelrislabs.com.
authors.forename: Arpita
authors.initials: A
authors.lastname: Ghosh
authors.affiliation: Department of Agricultural and Environmental Sciences, Milan State University, via G. Celoria 2, Milan 20133, Italy. marcello.iriti@unimi.it.
authors.forename: Marcello
authors.initials: M
authors.lastname: Iriti
mesh_terms: D000085:Acetates / Q000494:pharmacology*; D018533:Biomass; D053898:Biosynthetic Pathways / Q000187:drug effects / Q000235:genetics; D003517:Cyclopentanes / Q000494:pharmacology*; D004144:Diosgenin / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D020043:Genes, Essential; D017343:Genes, Plant; D054883:Oxylipins / Q000494:pharmacology*; D036226:Seedlings / Q000033:anatomy & histology / Q000187:drug effects / Q000378:metabolism*; D029910:Trigonella / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*
chemical_list: D000085:Acetates; D003517:Cyclopentanes; D054883:Oxylipins; C072239:methyl jasmonate; D004144:Diosgenin
keywords: 3-hydroxy-3-methylglutaryl-CoA reductase; elicitors; mevalonate pathway; plant innate immunity; steroidal saponins; sterol-3-β-glucosyl transferase