title: Life-history strategies and carbon metabolism gene dosage in the Nakaseomyces yeasts.
abstract: The Nakaseomyces clade consists of a group of six hemiascomyceteous yeasts (Candida glabrata, Nakaseomyces delphensis, C. nivarensis, C. bracarensis, C. castelli, N. bacillisporus), phylogenetically close to the yeast Saccharomyces cerevisiae, their representative being the well-known pathogenic yeast C. glabrata. Four species had been previously examined for their carbon assimilation properties and found to have similar properties to S. cerevisiae (repression of respiration in high glucose-i.e. Crabtree positivity-and being a facultative anaerobe). We examined here the complete set of the six species for their carbon metabolic gene content. We also measured different metabolic and life-history traits (glucose consumption rate, population growth rate, carrying capacity, cell size, cell and biomass yield). We observed deviations from the glycolytic gene redundancy observed in S. cerevisiae presumed to be an important property for the Crabtree positivity, especially for the two species C. castelli and N. bacillisporus which frequently have only one gene copy, but different life strategies. Therefore, we show that the decrease in carbon metabolic gene copy cannot be simply associated with a reduction of glucose consumption rate and can be counterbalanced by other beneficial genetic variations.
authors.affiliation: Univ Paris-Sud, UMR 0320/UMR8120 Génétique Quantitative et Evolution-Le Moulon, Université Paris-Saclay, F-91190 Gif-sur-Yvette, France.
authors.forename: Judith
authors.initials: J
authors.lastname: Legrand
authors.affiliation: CNRS UMR 8621 Institut de Génétique et Microbiologie, Univ Paris Sud F-91140 Orsay Cedex CNRS, UMR 0320/UMR8120 Génétique Quantitative et Evolution-Le Moulon, Université Paris-Saclay, F-91190 Gif-sur-Yvette, France monique.bolotin@u-psud.fr.
authors.forename: Monique
authors.initials: M
authors.lastname: Bolotin-Fukuhara
authors.affiliation: Univ Paris-Sud, UMR 0320/UMR8120 Génétique Quantitative et Evolution-Le Moulon, Université Paris-Saclay, F-91190 Gif-sur-Yvette, France.
authors.forename: Aurélie
authors.initials: A
authors.lastname: Bourgais
authors.affiliation: CNRS UMR 8621 Institut de Génétique et Microbiologie, Univ Paris Sud F-91140 Orsay Cedex CNRS, UMR 0320/UMR8120 Génétique Quantitative et Evolution-Le Moulon, Université Paris-Saclay, F-91190 Gif-sur-Yvette, France.
authors.forename: Cécile
authors.initials: C
authors.lastname: Fairhead
authors.affiliation: Univ Paris-Sud, UMR 0320/UMR8120 Génétique Quantitative et Evolution-Le Moulon, Université Paris-Saclay, F-91190 Gif-sur-Yvette, France INRA, UMR 1083 Sciences pour l'oenologie, 34060 Montpellier Cedex 2, France.
authors.forename: Delphine
authors.initials: D
authors.lastname: Sicard
mesh_terms: D000332:Aerobiosis; D000693:Anaerobiosis; D018533:Biomass; D002244:Carbon / Q000378:metabolism*; D005285:Fermentation; D018628:Gene Dosage; D005947:Glucose / Q000378:metabolism; D006019:Glycolysis; D053858:Metabolic Networks and Pathways / Q000235:genetics*; D010085:Oxidative Phosphorylation; D004718:Saccharomycetales / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*
chemical_list: D002244:Carbon; D005947:Glucose
keywords: Nakaseomyces; S. cerevisiae; carbon metabolism; gene duplication; life-history traits; metabolism