title: The effect of angiotensin-converting enzyme inhibition throughout a superovulation protocol in ewes.
abstract: Many studies identified new components of the renin–angiotensin system (RAS), such as Angiotensin-(1-7) [Ang-(1–7)] and Angiotensin-converting enzyme type 2 (ACE2), in mammalian ovaries.We previously showed Angiotensin-Converting Enzyme (ACE) inhibition, which increases the level of Ang-(1–7), stimulated ovarian estradiol output in ewe after estrous synchronization. Considering that Ang-(1–7) stimulates ovarian function and elevated estradiol before ovulation is associated with increased chance of achieving pregnancy, the present study investigated whether ACE inhibition throughout a superovulation protocol in ewe might improve ovulation outcome. At first, immunohistochemistry in ovaries of nonpregnant ewes revealed localization of Angiotensin II (Ang II), Ang-(1–7) and ACE2 in theca cells of antral follicles and in corpus luteum. Ang II and Ang-(1–7)were also detected in follicular fluid (FF) by Radioimmunoassay (RIA). Enalapril treatment throughout the superovulation protocol decreased 17β-estradiol (E2) output and raised progesterone:estradiol (P4:E2) ratio without a direct influence on ovulation and quality of embryos.
authors.affiliation: Universidade Federal do Piauí, Teresina, PI, Brazil.
authors.forename: Alécio Matos
authors.initials: AM
authors.lastname: Pereira
authors.affiliation: Universidade Federal do Piauí, Teresina, PI, Brazil.
authors.forename: Antônio
authors.initials: A
authors.lastname: de Souza Júnior
authors.affiliation: Departamento de Fisiologia e Biofísica, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerias, Belo Horizonte, MG, Brazil.
authors.forename: Fernanda Brandão
authors.initials: FB
authors.lastname: Machado
authors.affiliation: Departamento de Fisiologia e Biofísica, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerias, Belo Horizonte, MG, Brazil.
authors.forename: Gleisy Kelly Neves
authors.initials: GK
authors.lastname: Gonçalves
authors.affiliation: Universidade Federal do Piauí, Teresina, PI, Brazil.
authors.forename: Lauro César Soares
authors.initials: LC
authors.lastname: Feitosa
authors.affiliation: Departamento de Fisiologia e Biofísica, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerias, Belo Horizonte, MG, Brazil.
authors.forename: Adelina Martha
authors.initials: AM
authors.lastname: Reis
authors.affiliation: Departamento de Fisiologia e Biofísica, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerias, Belo Horizonte, MG, Brazil.
authors.forename: Robson Augusto Souza
authors.initials: RA
authors.lastname: Santos
authors.affiliation: Centro de Microscopia da Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil. Electronic address: kinulpe@yahoo.com.br.
authors.forename: Kinulpe
authors.initials: K
authors.lastname: Honorato-Sampaio
authors.affiliation: Universidade Federal do Piauí, Teresina, PI, Brazil. Electronic address: amilfox@uol.com.
authors.forename: Amilton Raposo
authors.initials: AR
authors.lastname: Costa
mesh_terms: D000803:Angiotensin I / Q000378:metabolism; D000804:Angiotensin II / Q000378:metabolism; D000085962:Angiotensin-Converting Enzyme 2; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000494:pharmacology*; D000818:Animals; D004625:Embryo, Nonmammalian / Q000187:drug effects / Q000196:embryology; D004656:Enalapril / Q000494:pharmacology*; D004958:Estradiol / Q000378:metabolism*; D005260:Female; D010060:Ovulation / Q000187:drug effects; D010446:Peptide Fragments / Q000378:metabolism; D007703:Peptidyl-Dipeptidase A / Q000378:metabolism; D011374:Progesterone / Q000378:metabolism*; D012756:Sheep / Q000502:physiology*; D013480:Superovulation / Q000187:drug effects*
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D010446:Peptide Fragments; D000804:Angiotensin II; D011374:Progesterone; D004958:Estradiol; D004656:Enalapril; D000803:Angiotensin I; D007703:Peptidyl-Dipeptidase A; D000085962:Angiotensin-Converting Enzyme 2; C118790:angiotensin I (1-7)
keywords: Angiotensin-(1-7); Enalapril; Ewes; Ovulation; Steroidogenesis