title: Roll-Plate Alone Does Not Demonstrate Colonization In Silicone Neonatal Catheters.
abstract: We compared roll-plate, sonication and slicing for the detection of colonization and catheter-related bloodstream infection (C-RBSI) in 90 silicone neonatal peripherally inserted central catheters. Colonization was detected by roll-plate, sonication and slicing in 3.4%, 6.9% and 10.3% of catheters, respectively. C-RBSI was detected by roll-plate, sonication and slicing only in 4.8% of each. The roll-plate technique was not a good predictor of colonization and C-RBSI in silicone neonatal peripherally inserted central catheters. Detecting intraluminal colonization was required.
authors.affiliation: From the *Department of Clinical Microbiology and Infectious Diseases, Hospital General Universitario Gregorio Marañón; †Medicine Department, School of Medicine, Universidad Complutense de Madrid; ‡CIBER Enfermedades Respiratorias-CIBERES (CB06/06/0058); and §Cardiac Surgery Postoperative Care Unit, Hospital General Universitario Gregorio Marañón, Madrid, Spain.
authors.forename: María
authors.initials: M
authors.lastname: Guembe
authors.forename: Pablo
authors.initials: P
authors.lastname: Martín-Rabadán
authors.forename: Raquel
authors.initials: R
authors.lastname: Cruces
authors.forename: María Jesús
authors.initials: MJ
authors.lastname: Pérez Granda
authors.forename: Emilio
authors.initials: E
authors.lastname: Bouza
mesh_terms: D016470:Bacteremia; D001431:Bacteriological Techniques; D055499:Catheter-Related Infections / Q000175:diagnosis* / Q000453:epidemiology / Q000382:microbiology*; D062905:Central Venous Catheters / Q000009:adverse effects* / Q000145:classification / Q000382:microbiology*; D006801:Humans; D007231:Infant, Newborn; D012680:Sensitivity and Specificity; D012828:Silicones; D013030:Spain / Q000453:epidemiology
chemical_list: D012828:Silicones