title: Minocycline does not evoke anxiolytic and antidepressant-like effects in C57BL/6 mice.
abstract: Minocycline is a broad-spectrum tetracycline antibiotic with multiple actions, including anti-inflammatory and neuroprotective effects, that was proposed as novel treatment for several psychiatric disorders including schizophrenia and depression. However, there are contradictory results regarding antidepressant effects of minocycline in rodent models. Additionally, the possible anxiolytic effect of minocycline is still poorly investigated. Therefore, we aimed to clarify in the present study the influence of minocycline on behavioral correlates of mood disorders in standard tests for depression and anxiety, the Porsolt Forced Swim Test (FST), Elevated O-Maze, Dark-Light Box Test and Openfield Test in adult C57BL/6 mice. We found, unexpectedly, that mice treated with minocycline (20-40mg/kg, i.p.) did not display antidepressant- or anxiolytic-like behavioral changes in contrast to mice treated with diazepam (0.5mg/kg, anxiety tests) or imipramine (20mg/kg, depressive-like behavior). These results are relevant for future studies, considering that C57BL/6 mice, the most widely used strain in pharmacological and genetic animal models, did not react as expected to the treatment regime applied.
authors.affiliation: RG Animal Models in Psychiatry, Department of Psychiatry and Psychotherapy, Central Institute of Mental Health Mannheim, Medical Faculty Mannheim, University of Heidelberg, Heidelberg, Germany; Molecular Physiology of Hearing, Hearing Research Centre Tübingen (THRC), Department of Otolaryngology, University of Tübingen, Tubingen, Germany. Electronic address: miriam.vogt@zi-mannheim.de.
authors.forename: M A
authors.initials: MA
authors.lastname: Vogt
authors.affiliation: RG Animal Models in Psychiatry, Department of Psychiatry and Psychotherapy, Central Institute of Mental Health Mannheim, Medical Faculty Mannheim, University of Heidelberg, Heidelberg, Germany.
authors.forename: A S
authors.initials: AS
authors.lastname: Mallien
authors.affiliation: RG Animal Models in Psychiatry, Department of Psychiatry and Psychotherapy, Central Institute of Mental Health Mannheim, Medical Faculty Mannheim, University of Heidelberg, Heidelberg, Germany.
authors.forename: N
authors.initials: N
authors.lastname: Pfeiffer
authors.affiliation: RG Animal Models in Psychiatry, Department of Psychiatry and Psychotherapy, Central Institute of Mental Health Mannheim, Medical Faculty Mannheim, University of Heidelberg, Heidelberg, Germany; Division of Pediatric Endocrinology, University Children's Hospital Heidelberg, Heidelberg, Germany.
authors.forename: I
authors.initials: I
authors.lastname: Inta
authors.affiliation: RG Animal Models in Psychiatry, Department of Psychiatry and Psychotherapy, Central Institute of Mental Health Mannheim, Medical Faculty Mannheim, University of Heidelberg, Heidelberg, Germany.
authors.forename: P
authors.initials: P
authors.lastname: Gass
authors.affiliation: RG Animal Models in Psychiatry, Department of Psychiatry and Psychotherapy, Central Institute of Mental Health Mannheim, Medical Faculty Mannheim, University of Heidelberg, Heidelberg, Germany.
authors.forename: D
authors.initials: D
authors.lastname: Inta
mesh_terms: D000818:Animals; D014151:Anti-Anxiety Agents / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000928:Antidepressive Agents / Q000494:pharmacology; D001008:Anxiety Disorders / Q000188:drug therapy; D003866:Depressive Disorder / Q000188:drug therapy; D003975:Diazepam / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical; D007099:Imipramine / Q000494:pharmacology; D008297:Male; D008810:Mice, Inbred C57BL; D008911:Minocycline / Q000494:pharmacology*; D009483:Neuropsychological Tests
chemical_list: D014151:Anti-Anxiety Agents; D000900:Anti-Bacterial Agents; D000928:Antidepressive Agents; D008911:Minocycline; D007099:Imipramine; D003975:Diazepam
keywords: Anxiety; Behavior; C57BL/6 mice; Depression; Locomotion; Minocycline