title: Application of Carbon-Microsphere-Modified Electrodes for Electrochemistry of Hemoglobin and Electrocatalytic Sensing of Trichloroacetic Acid.
abstract: By using the hydrothermal method, carbon microspheres (CMS) were fabricated and used for electrode modification. The characteristics of CMS were investigated using various techniques. The biocompatible sensing platform was built by immobilizing hemoglobin (Hb) on the micrometer-sized CMS-modified electrode with a layer of chitosan membrane. On the cyclic voltammogram, a couple of quasi-reversible cathodic and anodic peaks appeared, showing that direct electrochemistry of Hb with the working electrode was achieved. The catalytic reduction peak currents of the bioelectrode to trichloroacetic acid was established in the linear range of 2.0~70.0 mmol·L(-1) accompanied by a detection limit of 0.30 mmol·L(-1) (3σ). The modified electrode displayed favorable sensitivity, good reproducibility and stability, which suggests that CMS is promising for fabricating third-generation bioelectrochemical sensors.
authors.affiliation: Key Laboratory of Tropical Medicinal Plant Chemistry of Ministry of Education, College of Chemistry and Chemical Engineering, Hainan Normal University, Haikou 571158, China. Sdwangwencheng@126.com.
authors.forename: Wen-Cheng
authors.initials: WC
authors.lastname: Wang
authors.affiliation: Key Laboratory of Tropical Medicinal Plant Chemistry of Ministry of Education, College of Chemistry and Chemical Engineering, Hainan Normal University, Haikou 571158, China. 18289751071@163.com.
authors.forename: Li-Jun
authors.initials: LJ
authors.lastname: Yan
authors.affiliation: Key Laboratory of Tropical Medicinal Plant Chemistry of Ministry of Education, College of Chemistry and Chemical Engineering, Hainan Normal University, Haikou 571158, China. shifan0802@126.com.
authors.forename: Fan
authors.initials: F
authors.lastname: Shi
authors.affiliation: Key Laboratory of Tropical Medicinal Plant Chemistry of Ministry of Education, College of Chemistry and Chemical Engineering, Hainan Normal University, Haikou 571158, China. xueliangniu@163.com.
authors.forename: Xue-Liang
authors.initials: XL
authors.lastname: Niu
authors.affiliation: Key Laboratory of Tropical Medicinal Plant Chemistry of Ministry of Education, College of Chemistry and Chemical Engineering, Hainan Normal University, Haikou 571158, China. huangguolei1982@163.com.
authors.forename: Guo-Lei
authors.initials: GL
authors.lastname: Huang
authors.affiliation: Key Laboratory of Tropical Medicinal Plant Chemistry of Ministry of Education, College of Chemistry and Chemical Engineering, Hainan Normal University, Haikou 571158, China. caijuan2002@163.com.
authors.forename: Cai-Juan
authors.initials: CJ
authors.lastname: Zheng
authors.affiliation: Key Laboratory of Tropical Medicinal Plant Chemistry of Ministry of Education, College of Chemistry and Chemical Engineering, Hainan Normal University, Haikou 571158, China. sunwei@qust.edu.cn.
authors.forename: Wei
authors.initials: W
authors.lastname: Sun
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D002244:Carbon / Q000737:chemistry*; D055664:Electrochemical Techniques / Q000295:instrumentation* / Q000379:methods; D004566:Electrodes; D006454:Hemoglobins / Q000737:chemistry*; D055767:Immobilized Proteins / Q000737:chemistry; D016014:Linear Models; D008863:Microspheres; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014238:Trichloroacetic Acid / Q000032:analysis*
chemical_list: D006454:Hemoglobins; D055767:Immobilized Proteins; D014238:Trichloroacetic Acid; D002244:Carbon
keywords: carbon microsphere; direct electrochemistry; hemoglobin; trichloroacetic acid