title: Research of DNA repair genes polymorphism XRCC1 and XPD and the risks of thyroid cancer development in persons exposed to ionizing radiation after the Chornobyl disaster.
abstract: OBJECTIVE
  The objective of this work was to determine and compare the features of DNA repair gene XRCC1 Arg399Gln and XPD Lys751Gln polymorphisms in patients with thyroid cancer (TC), who were exposed to ionizing radiation (IR) as a result of the Chornobyl disaster, and in patients without exposure to ionizing radiation in history.
  
  
  METHODS
  Determination of gene XRCC1 Arg399Gln and XPD Lys751Gln polymorphisms was performed by polymerase chain reaction (PCR) in 102 patients with thyroid cancer: 38 people, who were exposed to ionizing radiation due to Chornobyl disaster (members of the accident, and evacuees and residents from controlled areas contaminated with radionuclides), 64 individuals without exposure to ionizing radiation in history and 41 persons residents of Ukraine without cancer pathology in the control group. For comparison of the data on spontaneous and radiation-associated thyroid cancer and settlement of allele frequencies differences and risk of cancer pathology were used the literature data on control groups of populations of Russia, Belarus and Poland.
  
  
  RESULTS
  Comparing to the literature data on XRCC1 Arg399Gln polymorphisms in radiation-exposed individuals without cancer pathology, the risk of thyroid cancer in homozygous minor allele XRCC1 Gln399Gln carriers, who were exposed to ionizing radiation was significantly increased: OR = 4,14, p = 0,001 (CI95 % 1,72-9,93). In homozygous carriers of the minor allele of the gene XPD Lys751Gln, exposed to IR, revealed increased risk of thyroid cancer: OR = 3,30, p = 0,05 (CI 95 % 0,82-14,14), when compared with the control group of Ukrainian population.
  
  
  CONCLUSIONS
  The carriage of homozygous minor allele Gln399Gln XRCC1 and XPD Gln751Gln of DNA repair genes is a risk factor for thyroid cancer under the influence of ionizing radiation in research group of Ukrainian population.
authors.affiliation: State Institution National Research Center for Radiation Medicine of National Academy of Medical Sciences of Ukraine, Melnikov str., 53, Kyiv, 04050, Ukraine.
authors.forename: V M
authors.initials: VM
authors.lastname: Shkarupa
authors.affiliation: State Institution Institute of Hereditary Pathology National Academy of Medical Sciences of Ukraine, Lviv 79000, Lysenka 31A.
authors.forename: S O
authors.initials: SO
authors.lastname: Henyk-Berezovska
authors.affiliation: Ukrainian Scientific and Practical Center of Endocrine Surgery, Transplantation of endocrine organs and tissues of the Ministry of Health of Ukraine, Klovsky descent, 13 a, m. Kyiv, 01021, Ukraine.
authors.forename: V O
authors.initials: VO
authors.lastname: Palamarchuk
authors.affiliation: State Institution National Research Center for Radiation Medicine of National Academy of Medical Sciences of Ukraine, Melnikov str., 53, Kyiv, 04050, Ukraine.
authors.forename: V V
authors.initials: VV
authors.lastname: Talko
authors.affiliation: State Institution National Research Center for Radiation Medicine of National Academy of Medical Sciences of Ukraine, Melnikov str., 53, Kyiv, 04050, Ukraine.
authors.forename: S V
authors.initials: SV
authors.lastname: Klymenko
keywords: Chornobyl disaster; XRCC1 and XPD gene polymorphisms; thyroid cancer