title: Development of a fast and simple test system for the semiquantitative protein detection in cerebrospinal liquids based on gold nanoparticles.
abstract: The fast and simple detection of increased protein concentrations in cerebrospinal liquids is preferable in the emergency medicine and it can help to avoid unnecessary laboratory work by an early classification of neurological diseases. Here a test system is developed which is based on the electrostatic interaction between negatively charged gold nanoparticles and proteins at pH values around 5. The test system can be adjusted in such a way that protein/nanoparticles aggregates are formed leading to a red-shift in the absorption spectrum of the nanoparticles suspension. At concentrations above 500 mg/l the color of the suspension changes from red via violet toward blue in a rather small concentration range from 500 to 1000 mg/l. Furthermore the influence of various parameters such as gold nanoparticle concentration, pH value and varying ion concentration in the sample on the test system is examined. Finally cerebrospinal liquids of a larger number of patients have been analyzed.
authors.affiliation: Biosystems Technology, Institute of Applied Life Sciences, University of Applied Sciences Wildau, 15745 Wildau, Germany. Electronic address: ggoebel@th-wildau.de.
authors.forename: Gero
authors.initials: G
authors.lastname: Göbel
authors.affiliation: LIMETEC Biotechnologies GmbH, 16761 Hennigsdorf, Germany.
authors.forename: Robert
authors.initials: R
authors.lastname: Lange
authors.affiliation: In.vent DIAGNOSTICA GmbH, 16761 Hennigsdorf, Germany.
authors.forename: Jörg-Michael
authors.initials: JM
authors.lastname: Hollidt
authors.affiliation: Biosystems Technology, Institute of Applied Life Sciences, University of Applied Sciences Wildau, 15745 Wildau, Germany.
authors.forename: Fred
authors.initials: F
authors.lastname: Lisdat
mesh_terms: D000818:Animals; D002556:Cerebrospinal Fluid Proteins / Q000032:analysis*; D019963:Clinical Chemistry Tests / Q000379:methods*; D006046:Gold / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D053768:Metal Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D013997:Time Factors
chemical_list: D002556:Cerebrospinal Fluid Proteins; D006046:Gold
keywords: Gold nanoparticles; Liquor cerebrospinalis; Point-of-care; Protein detection