title: Rhizobium puerariaesp. nov., an endophytic bacterium from the root nodules of the medicinal plantPueraria candolleivar.candollei.
abstract: A Gram-stain-negative, rod-shaped, motile bacterium, PC004T, was isolated from root nodules of the Thai medicinal plantPueraria candolleivar.candollei. 16S rRNA gene sequence analysis indicated that the strain is phylogenetically related to species in the genusRhizobium,showing highest similarity (96.6 %) withRhizobium mesosinicumHAMBI 3194T. The phylogenetic tree reconstructed based on 16S rRNA gene sequences showed that strain PC004Tforms a cluster withRhizobium petroleariumKCTC 23288T. Based onatpD,gyrBandrecAgene sequences, strain PC004Talso showed low similarity ( < 90 %) to reference strains. These phylogenetic data indicate that PC004Tmay represent a novel species. Strain PC004Talso exhibited low DNA-DNA relatedness withR. mesosinicumHAMBI 3194T(8.2 %) andR. petroleariumKCTC 23288T(26.3 %). The DNA G+C content of strain PC004Twas 64 mol%, which is within the range reported for the genusRhizobium. The major fatty acid of PC004Twas C18 : 1ω7cwith minor amounts of C16 : 0, C16 : 03-OH, C18 : 03-OH, C18 : 12-OH, C19 : 0cyclo ω8cand summed feature 2. The strain was able to grow at pH 12 and with up to 2 % (w/v) NaCl. Strain PC004Tdid not nodulate five tested legumes and thenifHandnodCgenes were not detected by PCR. Based on the physiological, chemotaxonomic and phenotypic data from this study, strain PC004Trepresents a novel species of the genusRhizobium, for which the nameRhizobium puerariaesp. nov. is proposed; the type strain is PC004T( = BCC 73740T= NBRC 110722T).
authors.affiliation: Department of Pharmaceutical Botany, Faculty of Pharmacy, Mahidol University, 447 Sri-Ayuthaya Road, Rajathevi, Bangkok 10400, Thailand.
authors.forename: Panitch
authors.initials: P
authors.lastname: Boonsnongcheep
authors.affiliation: Department of Pharmaceutical Botany, Faculty of Pharmacy, Mahidol University, 447 Sri-Ayuthaya Road, Rajathevi, Bangkok 10400, Thailand.
authors.forename: Sompop
authors.initials: S
authors.lastname: Prathanturarug
authors.affiliation: Graduate School of Infection Control Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan.|Kitasato Institute for Life Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo, 108-8641, Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Takahashi
authors.affiliation: Graduate School of Infection Control Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan.|Kitasato Institute for Life Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo, 108-8641, Japan.
authors.forename: Atsuko
authors.initials: A
authors.lastname: Matsumoto