title: Dethiosulfatarculus sandiegensisgen. nov., sp. nov., isolated from a methanogenic paraffin-degrading enrichment culture and emended description of the familyDesulfarculaceae.
abstract: A mesophilic deltaproteobacterium, designated strain SPRT, was isolated from a methanogenic consortium capable of degrading long-chain paraffins. Cells were motile, vibrio-shaped, and occurred singly, in pairs or in clusters. Strain SPRTdid not metabolize hydrocarbons but grew fermentatively on pyruvate and oxaloacetate and autotrophically with H2and CO2. Thiosulfate served as a terminal electron acceptor, but sulfate or sulfite did not. The organism required at least 10 g NaCl l- 1and a small amount of yeast extract (0.001%) for growth. Optimal growth was observed between 30 and 37 °C and a pH range from 6.0 to 7.2. The DNA G+C content of SPRT's genome was 52.02 mol%. Based on 16S rRNA gene sequence analysis, strain SPRTwas distinct from previously describedDeltaproteobacteria, exhibiting the closest affiliation toDesulfarculus baarsiiDSM 2075TandDesulfocarbo indianensisSCBMT, with only 91% similarity between their respective 16S gene sequences.In silicogenome comparison supported the distinctiveness between strain SPRTand bothDesulfocarbo indianensisSCBMTandDesulfarculus baarsiiDSM 2075T. Based on physiological differences, as well as phylogenetic and genomic comparisons, we propose to classify SPRTas the type strain ( = DSM 100305T= JCM 30857T) of a novel species of a new genus with the nameDethiosulfatarculus sandiegensisgen. nov., sp. nov.
authors.affiliation: Department of Microbiology and Plant Biology, University of Oklahoma, George Lynn Cross Hall, 770 Van Vleet Oval, Norman, OK 73019, USA.|Institute for Energy and the Environment, The University of Oklahoma, 100 East Boyd Street, Room 1510, Norman, OK 73019-1015, USA.
authors.forename: Irene A
authors.initials: IA
authors.lastname: Davidova
authors.affiliation: Department of Microbiology and Plant Biology, University of Oklahoma, George Lynn Cross Hall, 770 Van Vleet Oval, Norman, OK 73019, USA.
authors.forename: Boris
authors.initials: B
authors.lastname: Wawrik
authors.affiliation: Department of Microbiology and Plant Biology, University of Oklahoma, George Lynn Cross Hall, 770 Van Vleet Oval, Norman, OK 73019, USA.
authors.forename: Amy V
authors.initials: AV
authors.lastname: Callaghan
authors.affiliation: Department of Microbiology and Plant Biology, University of Oklahoma, George Lynn Cross Hall, 770 Van Vleet Oval, Norman, OK 73019, USA.|Institute for Energy and the Environment, The University of Oklahoma, 100 East Boyd Street, Room 1510, Norman, OK 73019-1015, USA.
authors.forename: Kathleen
authors.initials: K
authors.lastname: Duncan
authors.affiliation: Department of Microbiology and Plant Biology, University of Oklahoma, George Lynn Cross Hall, 770 Van Vleet Oval, Norman, OK 73019, USA.|Institute for Energy and the Environment, The University of Oklahoma, 100 East Boyd Street, Room 1510, Norman, OK 73019-1015, USA.
authors.forename: Christopher R
authors.initials: CR
authors.lastname: Marks
authors.affiliation: Department of Microbiology and Plant Biology, University of Oklahoma, George Lynn Cross Hall, 770 Van Vleet Oval, Norman, OK 73019, USA.|Institute for Energy and the Environment, The University of Oklahoma, 100 East Boyd Street, Room 1510, Norman, OK 73019-1015, USA.
authors.forename: Joseph M
authors.initials: JM
authors.lastname: Suflita