title: Indirect Manganese Removal by Stenotrophomonas sp. and Lysinibacillus sp. Isolated from Brazilian Mine Water.
abstract: Manganese is a contaminant in the wastewaters produced by Brazilian mining operations, and the removal of the metal is notoriously difficult because of the high stability of the Mn(II) ion in aqueous solutions. To explore a biological approach for removing excessive amounts of aqueous Mn(II), we investigated the potential of Mn(II) oxidation by both consortium and bacterial isolates from a Brazilian manganese mine. A bacterial consortium was able to remove 99.7% of the Mn(II). A phylogenetic analysis of isolates demonstrated that the predominant microorganisms were members of Stenotrophomonas, Bacillus, and Lysinibacillus genera. Mn(II) removal rates between 58.5% and 70.9% were observed for Bacillus sp. and Stenotrophomonas sp. while the Lysinibacillus isolate 13P removes 82.7%. The catalytic oxidation of Mn(II) mediated by multicopper oxidase was not properly detected; however, in all of the experiments, a significant increase in the pH of the culture medium was detected. No aggregates inside the cells grown for a week were found by electronic microscopy. Nevertheless, an energy-dispersive X-ray spectroscopy of the isolates revealed the presence of manganese in Stenotrophomonas sp. and Lysinibacillus sp. grown in K medium. These results suggest that members of Stenotrophomonas and Lysinibacillus genera were able to remove Mn(II) by a nonenzymatic pathway.
authors.affiliation: Laboratório de Bioquímica e Biologia Molecular, Departamento de Ciências Biológicas (DECBI) and Instituto de Ciências Exatas e Biológica (ICEB), Universidade Federal de Ouro Preto, 35400-000 Ouro Preto, MG, Brazil.
authors.forename: Natália Rocha
authors.initials: NR
authors.lastname: Barboza
authors.affiliation: Laboratório de Bioquímica e Biologia Molecular, Departamento de Ciências Biológicas (DECBI) and Instituto de Ciências Exatas e Biológica (ICEB), Universidade Federal de Ouro Preto, 35400-000 Ouro Preto, MG, Brazil.
authors.forename: Soraya Sander
authors.initials: SS
authors.lastname: Amorim
authors.affiliation: Laboratório de Bioquímica e Biologia Molecular, Departamento de Ciências Biológicas (DECBI) and Instituto de Ciências Exatas e Biológica (ICEB), Universidade Federal de Ouro Preto, 35400-000 Ouro Preto, MG, Brazil.
authors.forename: Pricila Almeida
authors.initials: PA
authors.lastname: Santos
authors.affiliation: Laboratório de Bio&Hidrometalurgia, Departamento de Engenharia Metalúrgica e de Materiais (DEMET), Escola de Minas, Universidade Federal de Ouro Preto, 35400-000 Ouro Preto, MG, Brazil.
authors.forename: Flávia Donária
authors.initials: FD
authors.lastname: Reis
authors.affiliation: Faculdade Unileste, 35160-215 Ipatinga, MG, Brazil.
authors.forename: Mônica Mendes
authors.initials: MM
authors.lastname: Cordeiro
authors.affiliation: Laboratório de Bioquímica e Biologia Molecular, Departamento de Ciências Biológicas (DECBI) and Instituto de Ciências Exatas e Biológica (ICEB), Universidade Federal de Ouro Preto, 35400-000 Ouro Preto, MG, Brazil.
authors.forename: Renata
authors.initials: R
authors.lastname: Guerra-Sá
authors.affiliation: Laboratório de Bio&Hidrometalurgia, Departamento de Engenharia Metalúrgica e de Materiais (DEMET), Escola de Minas, Universidade Federal de Ouro Preto, 35400-000 Ouro Preto, MG, Brazil.
authors.forename: Versiane Albis
authors.initials: VA
authors.lastname: Leão
mesh_terms: D001407:Bacillus / Q000737:chemistry / Q000378:metabolism*; D001673:Biodegradation, Environmental; D001938:Brazil; D002384:Catalysis; D006801:Humans; D008345:Manganese / Q000737:chemistry* / Q000633:toxicity; D010084:Oxidation-Reduction; D010802:Phylogeny; D020588:Stenotrophomonas / Q000737:chemistry / Q000378:metabolism*; D014877:Water Pollution, Chemical
chemical_list: D008345:Manganese