title: Treatment with PPARα Agonist Clofibrate Inhibits the Transcription and Activation of SREBPs and Reduces Triglyceride and Cholesterol Levels in Liver of Broiler Chickens.
abstract: PPARα agonist clofibrate reduces cholesterol and fatty acid concentrations in rodent liver by an inhibition of SREBP-dependent gene expression. In present study we investigated the regulation mechanisms of the triglyceride- and cholesterol-lowering effect of the PPARα agonist clofibrate in broiler chickens. We observed that PPARα agonist clofibrate decreases the mRNA and protein levels of LXRα and the mRNA and both precursor and nuclear protein levels of SREBP1 and SREBP2 as well as the mRNA levels of the SREBP1 (FASN and GPAM) and SREBP2 (HMGCR and LDLR) target genes in the liver of treated broiler chickens compared to control group, whereas the mRNA level of INSIG2, which inhibits SREBP activation, was increased in the liver of treated broiler chickens compared to control group. Taken together, the effects of PPARα agonist clofibrate on lipid metabolism in liver of broiler chickens involve inhibiting transcription and activation of SREBPs and SREBP-dependent lipogenic and cholesterologenic gene expression, thereby resulting in a reduction of the triglyceride and cholesterol levels in liver of broiler chickens.
authors.affiliation: Institute of Animal Husbandry and Veterinary Sciences, Shanxi Provincial Academy of Agricultural Sciences, Taiyuan 030031, China.
authors.forename: Lijun
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Institute of Animal Husbandry and Veterinary Sciences, Shanxi Provincial Academy of Agricultural Sciences, Taiyuan 030031, China.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Li
authors.affiliation: Institute of Animal Husbandry and Veterinary Sciences, Shanxi Provincial Academy of Agricultural Sciences, Taiyuan 030031, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.affiliation: Institute of Animal Husbandry and Veterinary Sciences, Shanxi Provincial Academy of Agricultural Sciences, Taiyuan 030031, China.
authors.forename: Shenghua
authors.initials: S
authors.lastname: Zhou
authors.affiliation: Institute of Animal Husbandry and Veterinary Sciences, Shanxi Provincial Academy of Agricultural Sciences, Taiyuan 030031, China.
authors.forename: Mingjun
authors.initials: M
authors.lastname: Shangguan
authors.affiliation: Institute of Animal Husbandry and Veterinary Sciences, Shanxi Provincial Academy of Agricultural Sciences, Taiyuan 030031, China.
authors.forename: Lina
authors.initials: L
authors.lastname: Xue
authors.affiliation: Institute of Animal Husbandry and Veterinary Sciences, Shanxi Provincial Academy of Agricultural Sciences, Taiyuan 030031, China.
authors.forename: Bianying
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Institute of Animal Husbandry and Veterinary Sciences, Shanxi Provincial Academy of Agricultural Sciences, Taiyuan 030031, China.
authors.forename: Fuxiang
authors.initials: F
authors.lastname: Ding
authors.affiliation: Institute of Biotechnology of Shanxi University, Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Taiyuan 030006, China.
authors.forename: Dequan
authors.initials: D
authors.lastname: Hui
authors.affiliation: Institute of Biotechnology of Shanxi University, Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Taiyuan 030006, China.
authors.forename: Aihua
authors.initials: A
authors.lastname: Liang
authors.affiliation: Institute of Animal Husbandry and Veterinary Sciences, Shanxi Provincial Academy of Agricultural Sciences, Taiyuan 030031, China.
authors.forename: Dongchang
authors.initials: D
authors.lastname: He