title: [Inhibitory effect of salinomycin on human breast cancer cells MDA-MB-231 proliferation through Hedgehog signaling pathway].
abstract: OBJECTIVE
  To investigate the inhibitory effect of salinomycin on human breast cancer cells in vitro, and to explore the related molecular mechanism.
  
  
  METHODS
  Human breast cancer MDA-MB-231 cells were treated with salinomycin at different concentrations and at various time points. The effect of salinomycin on MDA-MB-231 cells proliferation was studied by CCK-8 method. The cell cycle status was examined by flow cytometry. RT-PCR and Western blot were used to detect the expression of Shh, Smo and Gli1 in the Hedgehog pathway at mRNA and protein levels.
  
  
  RESULTS
  Proliferation of MDA-MB-231 cells treated with salinomycin was markedly inhibited in a concentration and time dependent manner. Salinomycin at concentrations of 0, 0.4, 0.8 and 1.6 µmol/L inhibited the growth at the rates of 11.18%, 25.88%, 50.03%, 92.65%, respectively. Salinomycin prevented MDA-MB-231 cells from G1 into S phase. Salinomycin at concentrations of 0, 0.8 and 1.6 µmol/L resulted in S-phase percentage of 25.03%, 11.85% and 35.21%, respectively (P < 0.05). RT-PCR and Western blot showed that the expression of key elements Shh, Smo and Gli1 in the Hedgehog pathway was inhibited by salinomycin in a concentration dependent manner (P < 0.05).
  
  
  CONCLUSIONS
  Salinomycin prevents breast cancer cell transition from G1 to S phase through downregulation of the target genes of Hedgehog signaling pathway, leading to an effective inhibition of MDA-MB-231 cells.
authors.affiliation: The Key Laboratory of Tumor Stem Cell Research of Liaoning Province, Dalian Medical University, Dalian 116044, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Lu
authors.affiliation: The Key Laboratory of Tumor Stem Cell Research of Liaoning Province, Dalian Medical University, Dalian 116044, China.
authors.forename: Chunying
authors.initials: C
authors.lastname: Zhang
authors.affiliation: The Key Laboratory of Tumor Stem Cell Research of Liaoning Province, Dalian Medical University, Dalian 116044, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Li
authors.affiliation: The Key Laboratory of Tumor Stem Cell Research of Liaoning Province, Dalian Medical University, Dalian 116044, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Mao
authors.affiliation: The Key Laboratory of Tumor Stem Cell Research of Liaoning Province, Dalian Medical University, Dalian 116044, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Ma
authors.affiliation: The Key Laboratory of Tumor Stem Cell Research of Liaoning Province, Dalian Medical University, Dalian 116044, China.
authors.forename: Xiaotang
authors.initials: X
authors.lastname: Yu
authors.affiliation: The Key Laboratory of Tumor Stem Cell Research of Liaoning Province, Dalian Medical University, Dalian 116044, China.
authors.forename: Zhenhuan
authors.initials: Z
authors.lastname: Hou
authors.affiliation: The Key Laboratory of Tumor Stem Cell Research of Liaoning Province, Dalian Medical University, Dalian 116044, China; E-mail: lilianhong9177@163.com.
authors.forename: Lianhong
authors.initials: L
authors.lastname: Li
mesh_terms: D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation; D005260:Female; D053823:Hedgehog Proteins / Q000378:metabolism*; D006801:Humans; D011714:Pyrans / Q000008:administration & dosage / Q000494:pharmacology*; D015398:Signal Transduction; D000072086:Smoothened Receptor / Q000378:metabolism; D013997:Time Factors; D000071676:Zinc Finger Protein GLI1 / Q000378:metabolism; D000095662:Polyether Polyketides
chemical_list: D053823:Hedgehog Proteins; D011714:Pyrans; D000072086:Smoothened Receptor; D000071676:Zinc Finger Protein GLI1; C400551:GLI1 protein, human; C509239:SHH protein, human; C491023:SMO protein, human; C010327:salinomycin; D000095662:Polyether Polyketides