title: IL-17 promoted the inhibition of medulloblastoma in mice by splenocyte injection.
abstract: BACKGROUND
  Interleukin 17 (IL-17) is a proinflammatory cytokine produced by a new subset of activated CD4+ T cells, Th17 cells. We previously showed that increased Th17 cell populations were presented in human medulloblastoma-infiltrating T cells and peripheral blood. In this study, we attempted to address the possible role of Th17 cells in the biologic activity of IL-17 for tumor control.
  
  
  METHODS
  We grafted fresh surgically obtained medulloblastoma into syngeneic athymic nude/nude mice. We intrapertonially injected splenocyte and murine IL-17 in mice on the second day. The tumor volume and the life spans of the mice were measured. Meanwhile, the IL-17, IL-6, IL-23, Ccl2, Ccl20 and IFN-gamma expression in the tumors was also examined by real-time PCR, Western blot and enzyme-linked immunosorbent assay.
  
  
  RESULTS
  We found that medulloblastoma growth in IL-17-injected mice was significantly inhibited compared to the non-IL-17 treated mice. In contrast to the IL-17 antitumor activity observed in mice injected with splenocytes, we observed that IFN-gamma, IL-6, IL-23, Ccl2, and Ccl20 proteins were significantly increased in tumor tissues of mice injected with IL-17.
  
  
  CONCLUSIONS
  These experiments suggest that IL-17 may promote splenocyte antitumor activity in medulloblastoma. We postulate that IL-17's antitumor activity may be related to the increased protein levels of IFN-gamma, IL-6, IL-23, Ccl2, and Ccl20.
authors.affiliation: Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai, 200040, China. neurozhou@126.com.
authors.forename: Ping
authors.initials: P
authors.lastname: Zhou
authors.affiliation: Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai, 200040, China. zhang19910925@163.com.
authors.forename: Qilin
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai, 200040, China. zhaoyaohs@vip.sina.com.
authors.forename: Yao
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Institute of Biomedical Sciences, Fudan University, Shanghai, 200032, China. shahongying@163.com.
authors.forename: Hongying
authors.initials: H
authors.lastname: Sha
authors.affiliation: Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai, 200040, China. Caoxiaoyun2015@126.com.
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Cao
authors.affiliation: Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai, 200040, China. eamns@hotmail.com.
authors.forename: Yongfei
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000494:pharmacology; D002528:Cerebellar Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D018932:Chemokine CCL2 / Q000378:metabolism; D054418:Chemokine CCL20 / Q000378:metabolism; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D007267:Injections; D007371:Interferon-gamma / Q000378:metabolism; D020381:Interleukin-17 / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology*; D053759:Interleukin-23 / Q000235:genetics; D015850:Interleukin-6 / Q000235:genetics; D008527:Medulloblastoma / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D013154:Spleen / Q000166:cytology; D014407:Tumor Cells, Cultured; D023041:Xenograft Model Antitumor Assays
chemical_list: D000970:Antineoplastic Agents; C493447:CCL20 protein, mouse; C491768:Ccl2 protein, mouse; D018932:Chemokine CCL2; D054418:Chemokine CCL20; D020381:Interleukin-17; D053759:Interleukin-23; D015850:Interleukin-6; D007371:Interferon-gamma