title: 3D Culture of MIN-6 Cells on Decellularized Pancreatic Scaffold: In Vitro and In Vivo Study.
abstract: Type 1 diabetes is an autoimmune disease which is due to the lack of β cells. The ideal therapy to cure the disease is pancreas transplantation, but its application is confined to a limited number of people due to the shortage of organ and the need for life-long immunosuppression. Regenerative medicine methods such as a tissue engineered pancreas seem to provide a useful method. In order to construct a microenvironment similar to the native pancreas that is suitable for not only cell growth but also cellular function exertion, a decellularized mouse pancreas was used as a natural 3D scaffold in this experiment. MIN-6 β cells were planted in the bioscaffold. The cell engraftment was verified by HE staining and SEM. Immunostaining procedures were performed to confirm the normal function of the engrafted cells. qRT-PCR demonstrated that insulin gene expression of the recellularized pancreas was upregulated compared with conventional plate-cultured cells. In vivo experiment was also accomplished to further evaluate the function of the recellularized bioscaffold and the result was inspiring. And beyond doubt this will bring new hope for type 1 diabetic patients.
authors.affiliation: Department of General Surgery, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, China.
authors.forename: Di
authors.initials: D
authors.lastname: Wu
authors.affiliation: Department of General Surgery, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Wan
authors.affiliation: Department of General Surgery, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Surgical Comprehensive Laboratory, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, China.
authors.forename: Yibing
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Department of General Surgery, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, China.
authors.forename: Tianxin
authors.initials: T
authors.lastname: Xu
authors.affiliation: Department of General Surgery, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, China.
authors.forename: Mingyan
authors.initials: M
authors.lastname: Zhu
authors.affiliation: Department of General Surgery, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, China.
authors.forename: Xiangjun
authors.initials: X
authors.lastname: Fan
authors.affiliation: Department of General Surgery, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, China.
authors.forename: Shajun
authors.initials: S
authors.lastname: Zhu
authors.affiliation: Surgical Comprehensive Laboratory, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, China.
authors.forename: Changchun
authors.initials: C
authors.lastname: Ling
authors.affiliation: Surgical Comprehensive Laboratory, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, China.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Li
authors.affiliation: Surgical Comprehensive Laboratory, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Lu
authors.affiliation: Surgical Comprehensive Laboratory, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhu
authors.affiliation: Department of General Surgery, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, China.
authors.forename: Pengcheng
authors.initials: P
authors.lastname: Zhou
authors.affiliation: Department of General Surgery, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, China ; Surgical Comprehensive Laboratory, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, China.
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Department of General Surgery, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, China.
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000818:Animals; D002460:Cell Line; D005786:Gene Expression Regulation; D006801:Humans; D007328:Insulin / Q000096:biosynthesis*; D050417:Insulin-Secreting Cells / Q000166:cytology / Q000378:metabolism*; D008297:Male; D051379:Mice; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D007328:Insulin