title: Depletion of tyrosyl-DNA phosphodiesterase 1α (MtTdp1α) affects transposon expression in Medicago truncatula.
abstract: The role of plant tyrosyl-DNA phosphodiesterase 1α in genome stability is studied using a Medicago truncatula MtTdp1α-depleted line. Lack of MtTdp1α results in a 39% reduction of methylated cytosines as compared to control. RNA-Seq analyses revealed that 11 DNA transposons and 22 retrotransposons were differentially expressed in the Tdp1α-2a line. Among them all, DNA transposons (MuDR, hAT, DNA3-11_Mad) and seven retrotransposons (LTR (Long Terminal Repeat)/Gipsy, LTR/Copia, LTR and NonLTR/L1) were down-regulated, while the 15 retrotransposons were up-regulated. Results suggest that the occurrence of stress-responsive cis-elements as well as changes in the methylation pattern at the LTR promoters might be responsible for the enhanced retrotransposon transcription.
authors.affiliation: Department of Biology and Biotechnology 'L. Spallanzani', via Ferrata 1, 27100 Pavia, Italy.
authors.forename: Maria Elisa
authors.initials: ME
authors.lastname: Sabatini
authors.affiliation: Department of Biology and Biotechnology 'L. Spallanzani', via Ferrata 1, 27100 Pavia, Italy.
authors.forename: Mattia
authors.initials: M
authors.lastname: Donà
authors.affiliation: Institute for Sustainable Plant Protection, National Research Council (CNR), Via Amendola 165/a, 70126, Bari, Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Leonetti
authors.affiliation: Department of Biotechnology, University of Verona, Strada Le Grazie 15, 37134, Verona, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Minio
authors.affiliation: Department of Biotechnology, University of Verona, Strada Le Grazie 15, 37134, Verona, Italy.
authors.forename: Massimo
authors.initials: M
authors.lastname: Delledonne
authors.affiliation: Department of Biology and Biotechnology 'L. Spallanzani', via Ferrata 1, 27100 Pavia, Italy.
authors.forename: Daniela
authors.initials: D
authors.lastname: Carboneral
authors.affiliation: Fodder and Dairy Production Research Centre, viale Piacenza 29, 29600, Lodi, Italy.
authors.forename: Massimo
authors.initials: M
authors.lastname: Confalonieri
authors.affiliation: Fodder and Dairy Production Research Centre, viale Piacenza 29, 29600, Lodi, Italy.
authors.forename: Giorgio
authors.initials: G
authors.lastname: Giraffa
authors.affiliation: Department of Biology and Biotechnology 'L. Spallanzani', via Ferrata 1, 27100 Pavia, Italy.
authors.forename: Alma
authors.initials: A
authors.lastname: Balestrazzi
mesh_terms: D003596:Cytosine / Q000378:metabolism; D019175:DNA Methylation / Q000235:genetics; D004251:DNA Transposable Elements / Q000235:genetics*; D017353:Gene Deletion; D018506:Gene Expression Regulation, Plant; D042822:Genomic Instability / Q000235:genetics; D046913:Medicago truncatula / Q000201:enzymology* / Q000235:genetics*; D010727:Phosphoric Diester Hydrolases / Q000235:genetics* / Q000378:metabolism; D018626:Retroelements / Q000235:genetics
chemical_list: D004251:DNA Transposable Elements; D018626:Retroelements; D003596:Cytosine; D010727:Phosphoric Diester Hydrolases; C122147:tyrosyl-DNA phosphodiesterase
keywords: DNA methylation; Medicago truncatula; RNA-Seq; transposable elements; tyrosyl-DNA phosphodiesterase