title: DNA-templated microwave-hydrothermal synthesis of nanostructured hydroxyapatite for storing and sustained release of an antibacterial protein.
abstract: Hydroxyapatite (HA) is promising in various biomedical applications owing to its similar chemical composition, structure and properties to the inorganic component in natural hard tissues. Herein, we report a DNA-templated microwave-assisted hydrothermal strategy for the preparation of HA nanostructured materials. As a kind of natural biomacromolecule, DNA molecules open up a new way to the synthesis of HA nanostructured materials with well-defined structures and morphologies. The HA nanostructured materials with a nanosheet-assembled hierarchical structure and a HA nanorod ordered structure are successfully prepared. The important roles of DNA molecules and pH values in the formation of HA nanostructured materials are investigated, and a possible formation mechanism is proposed. The as-prepared HA nanostructured materials exhibit a relatively high adsorption ability for chicken immunoglobulin Y (IgY) protein and a sustained protein release behavior. The as-prepared HA nanostructured materials after loading the IgY protein show a high antimicrobial activity. Thus, the HA nanostructured materials prepared by the DNA-templated microwave hydrothermal method are promising for the applications in various areas such as the prevention and treatment of dental caries.
authors.affiliation: Department of Preventive Dentistry, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, P. R. China.
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Preventive Dentistry, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, P. R. China.
authors.forename: Bin
authors.initials: B
authors.lastname: Yang
authors.affiliation: State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, P. R. China. y.j.zhu@mail.sic.ac.cn fchen@mail.sic.ac.cn.
authors.forename: Chao
authors.initials: C
authors.lastname: Qi
authors.affiliation: State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, P. R. China. y.j.zhu@mail.sic.ac.cn fchen@mail.sic.ac.cn.
authors.forename: Tuan-Wei
authors.initials: TW
authors.lastname: Sun
authors.affiliation: State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, P. R. China. y.j.zhu@mail.sic.ac.cn fchen@mail.sic.ac.cn.
authors.forename: Feng
authors.initials: F
authors.lastname: Chen
authors.affiliation: State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, P. R. China. y.j.zhu@mail.sic.ac.cn fchen@mail.sic.ac.cn.
authors.forename: Jin
authors.initials: J
authors.lastname: Wu
authors.affiliation: Department of Preventive Dentistry, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, P. R. China.
authors.forename: Xi-Ping
authors.initials: XP
authors.lastname: Feng
authors.affiliation: State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, P. R. China. y.j.zhu@mail.sic.ac.cn fchen@mail.sic.ac.cn.
authors.forename: Ying-Jie
authors.initials: YJ
authors.lastname: Zhu
mesh_terms: D000327:Adsorption; D000818:Animals; D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D002645:Chickens; D004247:DNA / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D017886:Durapatite / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D016967:Fusobacterium nucleatum / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D007136:Immunoglobulins / Q000737:chemistry*; D007779:Lactobacillus acidophilus / Q000187:drug effects; D046911:Macromolecular Substances / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D008872:Microwaves; D049329:Nanostructures / Q000737:chemistry*; D010316:Particle Size; D013295:Streptococcus mutans / Q000187:drug effects; D013329:Structure-Activity Relationship; D013499:Surface Properties; D013696:Temperature
chemical_list: D000900:Anti-Bacterial Agents; C016512:IgY; D007136:Immunoglobulins; D046911:Macromolecular Substances; D004247:DNA; D017886:Durapatite