title: Positively and negatively surface-charged chondroitin sulfate-trimethylchitosan nanoparticles as protein carriers.
abstract: Positively and negatively surface-charged nanoparticles (NPs) were prepared with chondroitin sulfate (ChS) and trimethylchitosan (TMC). NP size, surface charge, formation yield, and water content were investigated as a function of weight ratio and concentration. Size and zeta potential were controlled by varying the ChS/TMC mass ratio. FTIR spectra revealed interactions among composite NP constituents. TEM images showed that the NPs were nearly spherical, with an average size of ∼ 300 nm. Encapsulation efficiency increased in positively charged NPs with increases in fluorescein isothiocyanate-bovine serum albumin concentration. Negatively charged NPs had only 10-20% encapsulation efficiency. The release profile, release kinetics and mechanism of positively charged ChS-TMC NPs were studied in vitro. NP cytocompatibility and uptake were verified ex vivo. Both types of NPs were taken up and retained in cells. A549 cells took up more positively charged (49.4%) than negatively charged (35.5%) NPs.
authors.affiliation: Institute of Preventive Medicine, National Defense Medical Center, New Taipei City 23742, Taiwan, ROC.
authors.forename: Jenn-Jong
authors.initials: JJ
authors.lastname: Young
authors.affiliation: Institute of Preventive Medicine, National Defense Medical Center, New Taipei City 23742, Taiwan, ROC.
authors.forename: Cheng-Cheung
authors.initials: CC
authors.lastname: Chen
authors.affiliation: Institute of Preventive Medicine, National Defense Medical Center, New Taipei City 23742, Taiwan, ROC; Department of Physiology & Biophysics, National Defense Medical Center, Taipei City 11490, Taiwan, ROC.
authors.forename: Ying-Chuan
authors.initials: YC
authors.lastname: Chen
authors.affiliation: Institute of Preventive Medicine, National Defense Medical Center, New Taipei City 23742, Taiwan, ROC.
authors.forename: Kuang-Ming
authors.initials: KM
authors.lastname: Cheng
authors.affiliation: Institute of Preventive Medicine, National Defense Medical Center, New Taipei City 23742, Taiwan, ROC; Biomedical Engineering Program, Graduate Institute of Applied Science and Technology, National Taiwan University of Science and Technology, Taipei City 10607, Taiwan, ROC.
authors.forename: Hui-Ju
authors.initials: HJ
authors.lastname: Yen
authors.affiliation: Institute of Preventive Medicine, National Defense Medical Center, New Taipei City 23742, Taiwan, ROC.
authors.forename: Yu-Chuan
authors.initials: YC
authors.lastname: Huang
authors.affiliation: Division of Cardiology, Department of Internal Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei City 11490, Taiwan, ROC. Electronic address: genetic2001@hotmail.com.
authors.forename: Tsung-Neng
authors.initials: TN
authors.lastname: Tsai
mesh_terms: D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000031:analogs & derivatives*; D002809:Chondroitin Sulfates / Q000737:chemistry*; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D053769:Nanocapsules / Q000009:adverse effects / Q000737:chemistry*; D055672:Static Electricity
chemical_list: D053769:Nanocapsules; D002809:Chondroitin Sulfates; D048271:Chitosan
keywords: Cell uptake; Chondroitin sulfate; Cytotoxicity; Nanoparticles; Trimethylchitosan; Zeta potential