title: Effect of desiccation and resubmersion on the oxidative stress response of the kuruma shrimp Marsupenaeus japonicus.
abstract: In the present study, the oxidative stress response in hepatopancreas of Marsupenaeus japonicus to desiccation stress and resubmersed in seawater were studied, such as respiratory burst, ROS production ( [Formula: see text] ), activities of antioxidant enzymes (CAT, GPx, SOD, POD and GST) and oxidative damage to lipid and protein (indexed by contents of MDA). The duration of desiccation significantly influenced shrimp survival, and the mortality rates were 37.5% and 87.5% after desiccation 5 h and 10 h, respectively. After desiccation stress 3 h, the respiratory burst, ROS production, and the activity of SOD and CAT were up-regulated significantly. The activity of GPx and POD, and the content of MDA decreased significantly at 0.5 h and 1 h, and then increased significantly at 3 h. But GST activity was no significant change after desiccation. During the resubmersion period, most of the antioxidant enzymes activities could recover to the control level at 24 h, but a small quantity of the oxidative stress still existed in tissues. HE staining showed that desiccation stress induced damage symptoms in hepatopancreas of M. japonicus. These results revealed that desiccation influenced the antioxidative status and caused oxidative stress and tissue damage via confusion of antioxidant enzymes in M. japonicus, but the oxidative stress could be eliminated within a certain range after the shrimps were resubmersed in seawater.
authors.affiliation: Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, PR China.
authors.forename: Yafei
authors.initials: Y
authors.lastname: Duan
authors.affiliation: Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, PR China. Electronic address: jiasongzhang@hotmail.com.
authors.forename: Jiasong
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, PR China.
authors.forename: Hongbiao
authors.initials: H
authors.lastname: Dong
authors.affiliation: Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, PR China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, PR China.
authors.forename: Qingsong
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, PR China.
authors.forename: Hua
authors.initials: H
authors.lastname: Li
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D003890:Desiccation; D005260:Female; D043143:Hepatopancreas / Q000201:enzymology / Q000378:metabolism* / Q000648:ultrastructure; D007101:Immersion; D008136:Longevity; D008297:Male; D018384:Oxidative Stress; D033561:Penaeidae / Q000502:physiology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D016897:Respiratory Burst
chemical_list: D000975:Antioxidants; D017382:Reactive Oxygen Species
keywords: Antioxidants; Desiccation; Marsupenaeus japonicus; Oxidative stress; Resubmersion