title: Evaluation of Aloevera Gel as a Storage Medium in Maintaining the Viability of Periodontal Ligament Cells - An in Vitro Study.
abstract: OBJECTIVE
  To investigate the effectiveness of aloevera gel as a new storage medium in maintaining the viability of periodontal ligament cells.
  
  
  METHODS
  Premolars extracted for orthodontic reason were obtained. Confluent monolayers of fibroblasts were grown by cell culture method from the PDL cells isolated from the extracted teeth. One ml of this cell suspension was transferred to wells of culture plates, incubated for 24 hrs, followed by exposure to the three experimental media, Hank's balanced salt solution (HBSS), aloevera gel, and packaged drinking water. These plates were then assessed for viable cells using trypan blue dye exclusion test with haemocytometer after 15, 30, 60, 90 and 120 mins. The results obtained were statistically analysed using one-way analysis of variance (ANOVA).
  
  
  RESULTS
  At 15 min, HBSS presented maximum mean percentage of viable PDL cells (89%), followed by aloevera at 81% and packaged drinking water at 10%. Aloevera demonstrated 71%, 59%, 57% viable cells at 30, 60, 90 mins respectively. At 120 min, HBSS presented 57% viable cells followed by aloevera gel (45%) and packaged drinking water (3%). No statistical significant difference was observed between HBSS and aloevera gel.
  
  
  CONCLUSIONS
  Within the parameters of this study, both aloevera gel and HBSS were effective in maintaining the viability of PDL cells. Hence, aloevera gel could be used as a storage media for avulsed tooth in situations where availability of HBSS is in question.
authors.affiliation: From the Deptartment of Pedodontics and Preventive Dentistry , sawangi, Mahartashtra state, India.
authors.forename: Punit
authors.initials: P
authors.lastname: Fulzele
authors.affiliation: From the Deptartment of Pedodontics and Preventive Dentistry , sawangi, Mahartashtra state, India.
authors.forename: Sudhindra
authors.initials: S
authors.lastname: Baliga
authors.affiliation: From the Deptartment of Pedodontics and Preventive Dentistry , sawangi, Mahartashtra state, India.
authors.forename: Nilima
authors.initials: N
authors.lastname: Thosar
authors.affiliation: From the Deptartment of Pedodontics and Preventive Dentistry , sawangi, Mahartashtra state, India.
authors.forename: Debaprya
authors.initials: D
authors.lastname: Pradhan
mesh_terms: D000504:Aloe; D002452:Cell Count; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004396:Coloring Agents; D005347:Fibroblasts / Q000187:drug effects; D005782:Gels; D006801:Humans; D007552:Isotonic Solutions / Q000627:therapeutic use; D008422:Materials Testing; D019188:Organ Preservation Solutions / Q000627:therapeutic use*; D010513:Periodontal Ligament / Q000166:cytology*; D028321:Plant Preparations / Q000627:therapeutic use*; D013997:Time Factors; D014021:Tissue Preservation; D014343:Trypan Blue; D014867:Water
chemical_list: C586178:Aloe vera gel; D004396:Coloring Agents; D005782:Gels; C072670:Hanks Balanced Salt Solution; D007552:Isotonic Solutions; D019188:Organ Preservation Solutions; D028321:Plant Preparations; D014867:Water; D014343:Trypan Blue
keywords: Aloevera gel; HBSS; PDL viability; cell culture; storage media