title: INO1transcriptional memory leads to DNA zip code-dependent interchromosomal clustering.
abstract: Many genes localize at the nuclear periphery through physical interaction with the nuclear pore complex (NPC). We have found that the yeastINO1gene is targeted to the NPC both upon activation and for several generations after repression, a phenomenon called epigenetic transcriptional memory. Targeting ofINO1to the NPC requires distinctcis-acting promoter DNA zip codes under activating conditions and under memory conditions. When at the nuclear periphery, activeINO1clusters with itself and with other genes that share the GRS I zip code. Here, we show that during memory, the two alleles ofINO1cluster in diploids and endogenousINO1clusters with an ectopicINO1in haploids. After repression,INO1does not cluster with GRS I - containing genes. Furthermore, clustering during memory requires Nup100 and two sets of DNA zip codes, those that targetINO1to the periphery when active and those that target it to the periphery after repression. Therefore, the interchromosomal clustering ofINO1that occurs during transcriptional memory is dependent upon, but mechanistically distinct from, the clustering of activeINO1. Finally, while localization to the nuclear periphery is not regulated through the cell cycle during memory, clustering ofINO1during memory is regulated through the cell cycle.
authors.affiliation: Department of Molecular Biosciences, Northwestern University, Evanston, IL USA 60201.
authors.forename: Donna Garvey
authors.initials: DG
authors.lastname: Brickner
authors.affiliation: Department of Molecular Biosciences, Northwestern University, Evanston, IL USA 60201.
authors.forename: Robert
authors.initials: R
authors.lastname: Coukos
authors.affiliation: Department of Molecular Biosciences, Northwestern University, Evanston, IL USA 60201.
authors.forename: Jason H
authors.initials: JH
authors.lastname: Brickner
keywords: DNA zip code; epigenetic inheritance; interchromosomal clustering; nuclear pore; transcriptional memory