title: Aptamer-based microcantilever biosensor for ultrasensitive detection of tumor marker nucleolin.
abstract: We present an aptamer-based microcantilever biosensor for label-free detection of nucleolin. The sensor cantilevers in the microcantilever array were functionalized with nucleolin aptamer (AS1411) while the reference cantilevers were modified by 6-mercapto-1-hexanol (MCH) to eliminate environmental disturbances. The interaction between nucleolin and AS1411 induced surface stress changes, resulting in a differential deflection between sensor and reference cantilevers. The amplitude of differential cantilever deflection had a good linear relationship with the nucleolin concentration ranging from 10 nM to 250 nM with a correlation coefficient of 0.999. The detection limit was about 1.0 nM, at a signal-to-noise ratio of 3. The aptamer-based microcantilever sensor demonstrated good selectivity and was facile, rapid, and reagentless. Our results show the potential for the application of microcantilever biosensor system as a powerful tool to detect tumor markers with high sensitivity and specificity.
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China; School of Public Health, Jilin University, Changchun 130021, PR China.
authors.forename: Huiyan
authors.initials: H
authors.lastname: Li
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Bai
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
authors.forename: Nan
authors.initials: N
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
authors.forename: Xuejuan
authors.initials: X
authors.lastname: Chen
authors.affiliation: School of Public Health, Jilin University, Changchun 130021, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China. Electronic address: jltang@ciac.ac.cn.
authors.forename: Jilin
authors.initials: J
authors.lastname: Tang
mesh_terms: D052157:Aptamers, Nucleotide / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000032:analysis* / Q000378:metabolism; D015374:Biosensing Techniques / Q000295:instrumentation*; D057230:Limit of Detection; D010750:Phosphoproteins / Q000032:analysis* / Q000378:metabolism; D011188:Potassium / Q000494:pharmacology; D011485:Protein Binding / Q000187:drug effects; D016601:RNA-Binding Proteins / Q000032:analysis* / Q000378:metabolism; D000097796:Nucleolin
chemical_list: D052157:Aptamers, Nucleotide; D014408:Biomarkers, Tumor; D010750:Phosphoproteins; D011188:Potassium; D016601:RNA-Binding Proteins; D000097796:Nucleolin
keywords: AS1411; Aptamer; Microcantilever biosensor; Nucleolin; Tumor markers