title: Streptomyces yangpuensissp. nov., isolated from soil.
abstract: A novel Gram-stain-positive strain with sandy aerial mycelium and golden yellow substrate mycelium, designated fd2-tbT, was isolated from a soil sample collected in Shanghai, China, and its taxonomic status was established by phylogenetic analysis. 16S rRNA gene sequence analysis showed that strain fd2-tbTbelonged to the genusStreptomycesand was related toStreptomyces amritsarensisJCM 19660T(99.9 % 16S rRNA gene sequence similarity),Streptomyces flavotriciniNBRC 12770T(99.9 %),Streptomyces polychromogenesNBRC 13072T(99.8 %),Streptomyces racemochromogenesNRRL B-5430T(99.7 %),Streptomyces globosusLMG 19896T(99.5 %),Streptomyces toxytriciniNBRC 12823T(99.5 %) andStreptomyces katraeNBRC 13447T(99.3 %). The cell wall of strain fd2-tbTcontained ll-diaminopimelic acid, and whole-cell sugars were identified as glucose and ribose. The menaquinones MK-9(H4), MK-9(H6) and MK-9(H8) were also detected. In addition, the polar lipids diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, as well as five unidentified phospholipids, were detected. Major cellular fatty acids were identified as anteiso-C15 : 0, iso-C16 : 0, anteiso-C17 : 0, iso-C15 : 0and C16 : 0. DNA-DNA hybridization experiments showed that strain fd2-tbTexhibited 36.5 ± 0.6 %, 43.5 ± 2.0 %, 11.1 ± 1.3 %, 10.3 ± 3.1 %, 9.8 ± 1.9 %, 48.9 ± 3.9 % and 16.3 ± 1.7 % relatedness toS. amritsarensisJCM 40119660T,S. flavotriciniNBRC 12770T,S. polychromogenesNBRC 13072T,S. racemochromogenesNRRL B-5430T,S. globosusLMG 19896T,S. toxytriciniNBRC 12823TandS. katraeNBRC 13447T, respectively. Based on these analyses as well as some phenotypic differences, strain fd2-tbTis considered to represent a novel species of the genusStreptomyces, for which the nameStreptomyces yangpuensissp. nov. is proposed. The type strain is fd2-tbT( = DSM 100336T= CGMCC 4.7256T).
authors.affiliation: Collaborative Innovation Center for Genetics and Development, State Key Laboratory of Genetic Engineering, Department of Microbiology, School of Life Sciences, Fudan University, Shanghai, 200438, PRChina.
authors.forename: Biao
authors.initials: B
authors.lastname: Tang
authors.affiliation: Collaborative Innovation Center for Genetics and Development, State Key Laboratory of Genetic Engineering, Department of Microbiology, School of Life Sciences, Fudan University, Shanghai, 200438, PRChina.
authors.forename: Yucong
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education and the Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, PRChina.
authors.forename: Xiaoyang
authors.initials: X
authors.lastname: Zhi
authors.affiliation: Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education and the Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, PRChina.
authors.forename: Lingling
authors.initials: L
authors.lastname: Yang
authors.affiliation: CAS Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200032, PRChina.
authors.forename: Xufeng
authors.initials: X
authors.lastname: Cen
authors.affiliation: CAS Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200032, PRChina.|Collaborative Innovation Center for Genetics and Development, State Key Laboratory of Genetic Engineering, Department of Microbiology, School of Life Sciences, Fudan University, Shanghai, 200438, PRChina.
authors.forename: Guoping
authors.initials: G
authors.lastname: Zhao
authors.affiliation: Collaborative Innovation Center for Genetics and Development, State Key Laboratory of Genetic Engineering, Department of Microbiology, School of Life Sciences, Fudan University, Shanghai, 200438, PRChina.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Ding