title: Characterization of a novel negevirus isolated from Aedes larvae collected in a subarctic region of Japan.
abstract: We isolated and characterized a novel positive-sense, single-stranded RNA virus from Aedes larvae collected on Okushiri Island, Hokkaido, Japan. This virus, designated Okushiri virus (OKV), replicated in the Aedes albopictus cell line C6/36 with severe cytopathic effects and produced a large number of spherical viral particles that were 50-70 nm in diameter and released into the cell culture medium. The OKV genome consisted of 9,704 nucleotides, excluding the poly(A) tail at the 3'-terminus, and contained three major open reading frames (ORF1, ORF2, and ORF3). ORF1 encoded a putative protein of approximately 268 kDa that included a methyltransferase domain, FtsJ-like methyltransferase domain, helicase domain, and RNA-dependent RNA polymerase domain. The genome organization and results of a phylogenetic analysis based on the amino acid sequence predicted from the nucleotide sequence indicated that OKV is a member of a new insect virus group of negeviruses with a possible evolutionary relationship to some plant viruses. ORF2 and ORF3 were suggested to encode hypothetical membrane-associated proteins of approximately 45 kDa and 22 kDa, respectively. This is the first study on a novel negevirus isolated from mosquito larvae in Japan.
authors.affiliation: Laboratory of Applied Molecular Entomology, Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Sapporo, Japan.
authors.forename: Kota
authors.initials: K
authors.lastname: Kawakami
authors.affiliation: Laboratory of Applied Molecular Entomology, Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Sapporo, Japan.
authors.forename: Yudistira Wahyu
authors.initials: YW
authors.lastname: Kurnia
authors.affiliation: Laboratory of Applied Molecular Entomology, Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Sapporo, Japan.
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Fujita
authors.affiliation: Electron microscope Laboratory, Research Faculty of Agriculture, Hokkaido University, Sapporo, Japan.
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Ito
authors.affiliation: Department of Medical Entomology, National Institute of Infectious Diseases, Shinjuku, Tokyo, Japan.
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Isawa
authors.affiliation: Laboratory of Applied Molecular Entomology, Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Sapporo, Japan.
authors.forename: Shin-Ichiro
authors.initials: S
authors.lastname: Asano
authors.affiliation: Institute of Biotechnology, Vietnamese Academy of Science and Technology, Hanoi, Vietnam.
authors.forename: Ngo Dinh
authors.initials: ND
authors.lastname: Binh
authors.affiliation: Laboratory of Applied Molecular Entomology, Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Sapporo, Japan. hban@abs.agr.hokudai.ac.jp.
authors.forename: Hisanori
authors.identifier: http://orcid.org/0000-0001-6159-2810
authors.initials: H
authors.lastname: Bando
mesh_terms: D000330:Aedes / Q000821:virology*; D000595:Amino Acid Sequence; D063147:Animal Distribution; D000818:Animals; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D015967:Gene Expression Regulation, Viral / Q000502:physiology; D016679:Genome, Viral; D007304:Insect Viruses / Q000145:classification / Q000302:isolation & purification*; D007564:Japan; D008969:Molecular Sequence Data; D010802:Phylogeny; D012328:RNA Viruses / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D012367:RNA, Viral / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins