title: Quantum dots encoded Au coated polystyrene bead arranged micro-channel for multiplex arrays.
abstract: This paper describes a promising micro-channel multiplex immunoassay method based on the quantum dots encoded beads which requires micro-volume sample. Briefly, Au nanoparticles coated polystyrene (PS) beads were prepared and Quantum dots (QDs) were employed to encode 4 types of the PS beads by different emission wavelength QDs and various intensities. Different coding types of the beads were immobilized with different antibodies on the surface and BSA was used to block the unsatisfied sites. The antibody linked beads were then arranged in the 150 µm diameter optical capillary where the specific reactions took place before the detections. Results showed that the antibody on the Au coated surface maintains the bioactivity for the immunoreactions. Using this system, the fluorescent intensity was linear with analyte concentration in the range of 1×10(-7)-1×10(-5) mg/mL (RSD<5%, 4 repeats) and the lower detection limit reached 5×10(-8) mg/mL. It was proved to be a promising approach for the future miniaturization analytical devices.
authors.affiliation: Key Laboratory of Optoelectronic Chemical Materials and Devices of Ministry of Education, Jianghan University, Wuhan 430056, P. R. China; Flexible Display Materials and Technology Co-Innovation Centre of Hubei Province, Jianghan University, Wuhan 430056, P. R. China. Electronic address: yuancheng.cao@jhun.edu.cn.
authors.forename: Yuan-Cheng
authors.initials: YC
authors.lastname: Cao
authors.affiliation: Department of Chemical and Environmental Engineering, Wuhan Polytechnic University, Wuhan, Hubei Province, 430023, P. R. China. Electronic address: zoulljhun@126.com.
authors.forename: Zhan
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Key Laboratory of Optoelectronic Chemical Materials and Devices of Ministry of Education, Jianghan University, Wuhan 430056, P. R. China.
authors.forename: Runyu
authors.initials: R
authors.lastname: Yang
authors.affiliation: Key Laboratory of Optoelectronic Chemical Materials and Devices of Ministry of Education, Jianghan University, Wuhan 430056, P. R. China; Flexible Display Materials and Technology Co-Innovation Centre of Hubei Province, Jianghan University, Wuhan 430056, P. R. China.
authors.forename: Linling
authors.initials: L
authors.lastname: Zou
authors.affiliation: Key Laboratory of Optoelectronic Chemical Materials and Devices of Ministry of Education, Jianghan University, Wuhan 430056, P. R. China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: Hubei Key Laboratory of Industrial Fume and Dust Pollution Control, Jianghan University, Wuhan 430056, P. R. China.
authors.forename: Tie
authors.initials: T
authors.lastname: Mi
authors.affiliation: Key Laboratory of Optoelectronic Chemical Materials and Devices of Ministry of Education, Jianghan University, Wuhan 430056, P. R. China.
authors.forename: Hong
authors.initials: H
authors.lastname: Shi
keywords: Biochips; Capillary immunoassays; Fluorescent analysis; Multiplex; Quantum dots