title: Evaluation of the Acquisition of the Aerobic Metabolic Capacity by Myelin, during its Development.
abstract: Our previous reports indicate that the electron transfer chain and FoF1-ATP synthase are functionally expressed in myelin sheath, performing an extra-mitochondrial oxidative phosphorylation (OXPHOS), which would provide energy to the nerve axon. This supports the idea that myelin plays a trophic role for the axon. Although the four ETC complexes and ATP synthase are considered exquisite mitochondrial proteins, they are found ectopically expressed in several membranous structures. This study was designed to understand when and how the mitochondrial OXPHOS machinery is embedded in myelin, following myelinogenesis in the rat, which starts at birth and continues until the first month of age. Rats were sacrificed at different time points (from day 5 to 90 post birth). Western blot, immunofluorescence microscopy, luminometric, and oximetric analyses show that the isolated myelin starts to show OXPHOS components around the 11th day after birth and increases proportionally to the rat age, becoming similar to those of adult rat around the 30-third day. Interestingly, WB data show the same temporal relationship between myelinogenesis and appearance of proteins involved in mitochondrial fusion and cellular trafficking. It may be speculated that the OXPHOS complexes may be transferred to the endoplasmic reticulum membrane (known to interact with mitochondria) and from there through the Golgi apparatus to the forming myelin membrane.
authors.affiliation: Department of Pharmacy (DIFAR), Biochemistry Laboratory, University of Genova, Viale Benedetto XV 3, 16132, Genova, Italy. silvia.ravera@gmail.com.
authors.forename: Silvia
authors.initials: S
authors.lastname: Ravera
authors.affiliation: Department of Pharmacy (DIFAR), Biochemistry Laboratory, University of Genova, Viale Benedetto XV 3, 16132, Genova, Italy.
authors.forename: Martina
authors.initials: M
authors.lastname: Bartolucci
authors.affiliation: Department of Neuroscience, Rehabilitation, Ophthalmology, Genetics, Maternal and Child Health, University of Genova, Via de Toni 5, 16132, Genova, Italy.
authors.forename: Patrizia
authors.initials: P
authors.lastname: Garbati
authors.affiliation: DISTAV, University of Genova, C.so Europa 26, 16132, Genova, Italy.
authors.forename: Sara
authors.initials: S
authors.lastname: Ferrando
authors.affiliation: Department of Pharmacy (DIFAR), Biochemistry Laboratory, University of Genova, Viale Benedetto XV 3, 16132, Genova, Italy.
authors.forename: Daniela
authors.initials: D
authors.lastname: Calzia
authors.affiliation: DISTAV, University of Genova, C.so Europa 26, 16132, Genova, Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Ramoino
authors.affiliation: Department of Neuroscience, Rehabilitation, Ophthalmology, Genetics, Maternal and Child Health, University of Genova, Via de Toni 5, 16132, Genova, Italy.
authors.forename: Maurizio
authors.initials: M
authors.lastname: Balestrino
authors.affiliation: Department of Pharmacy (DIFAR), Biochemistry Laboratory, University of Genova, Viale Benedetto XV 3, 16132, Genova, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Morelli
authors.affiliation: Department of Pharmacy (DIFAR), Biochemistry Laboratory, University of Genova, Viale Benedetto XV 3, 16132, Genova, Italy.
authors.forename: Isabella
authors.initials: I
authors.lastname: Panfoli
mesh_terms: D000332:Aerobiosis; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D001921:Brain / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000378:metabolism; D006056:Golgi Apparatus / Q000378:metabolism; D008163:Luminescent Measurements; D008928:Mitochondria / Q000378:metabolism; D009186:Myelin Sheath / Q000378:metabolism*; D055495:Neurogenesis; D010085:Oxidative Phosphorylation; D010092:Oximetry; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D015415:Biomarkers
keywords: Endoplasmic reticulum; Energy metabolism; Golgi apparatus; Mitochondria; Myelinogenesis; OXPHOS