title: Enhanced Expression of Contractile-Associated Proteins and Ion Channels in Preterm Delivery Model Mice With Chronic Odontogenic Porphyromonas Gingivalis Infection.
abstract: Inflammation and infection have been reported to induce preterm delivery. We have studied the relationship between inflammation and various ion channels, including the L-type Ca(2+) channel and P2X7 receptor, during acute inflammation of the pregnant rat uterus induced by lipopolysaccharides. Recently, we found that mice with odontogenic Porphyromonas gingivalis (P.g, an important odontogenic pathogen) infection delivered at day 18.3 of gestation (vs. day 20.5 in normal mice). The purpose of this study was to investigate the expression of myometrial contractile-associated proteins inducing contractions and confirm that these mice are useful as a model for preterm delivery induced by chronic inflammation. We examined the expression of the oxytocin receptor, connexin 43, prostaglandin F receptors, L-type Ca(2+) channel, and P2X7 receptor in the myometrium at day 18 of gestation by real-time PCR and western blot analyses. We also measured TNF-α and IL-1β levels in the blood serum, placenta, fetal membrane and myometrium on the same day. mRNA expression of the oxytocin receptor, connexin 43, prostaglandin F receptors, L-type Ca(2+) channel, and P2X7 receptor was elevated by 5.4, 3.2, 2.4, 2.5, and 1.7 fold, respectively, in the P.g-infected mice. Protein levels of the oxytocin receptor and connexin 43 also increased. Serum levels of TNF-α and IL-1β were elevated, showing that systemic inflammation continued during pregnancy. IL-1β levels in the placenta and fetal membrane also increased, suggesting inflammatory reactions were induced. Thus, mice with odontogenic infection may be useful as a model of chronic inflammation-induced preterm delivery.
authors.affiliation: Department of Obstetrics and Gynecology, Graduate School of Biomedical & Health Sciences, Hiroshima University, Hiroshima, Japan miyo36@hiroshima-u.ac.jp.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Miyoshi
authors.affiliation: Department of Obstetrics and Gynecology, Graduate School of Biomedical & Health Sciences, Hiroshima University, Hiroshima, Japan.
authors.forename: Haruhisa
authors.initials: H
authors.lastname: Konishi
authors.affiliation: Department of Obstetrics and Gynecology, Graduate School of Biomedical & Health Sciences, Hiroshima University, Hiroshima, Japan.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Teraoka
authors.affiliation: Department of Obstetrics and Gynecology, Graduate School of Biomedical & Health Sciences, Hiroshima University, Hiroshima, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Urabe
authors.affiliation: Department of Oral and Maxillofacial Pathobiology, Basic Life Sciences, Institute of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan.
authors.forename: Hisako
authors.initials: H
authors.lastname: Furusho
authors.affiliation: Department of Oral and Maxillofacial Pathobiology, Basic Life Sciences, Institute of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan.
authors.forename: Mutsumi
authors.initials: M
authors.lastname: Miyauchi
authors.affiliation: Department of Oral and Maxillofacial Pathobiology, Basic Life Sciences, Institute of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Takata
authors.affiliation: Department of Obstetrics and Gynecology, Graduate School of Biomedical & Health Sciences, Hiroshima University, Hiroshima, Japan.
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Kudo
mesh_terms: D000818:Animals; D016866:Bacteroidaceae Infections / Q000378:metabolism*; D003285:Contractile Proteins / Q000378:metabolism*; D016207:Cytokines / Q000097:blood; D004195:Disease Models, Animal; D005260:Female; D007249:Inflammation / Q000097:blood / Q000378:metabolism* / Q000382:microbiology; D053583:Interleukin-1beta / Q000378:metabolism; D007473:Ion Channels / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D009215:Myometrium / Q000378:metabolism*; D010920:Placenta / Q000382:microbiology; D016966:Porphyromonas gingivalis / Q000378:metabolism; D011247:Pregnancy; D047928:Premature Birth / Q000378:metabolism* / Q000382:microbiology; D012333:RNA, Messenger / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D014590:Uterine Contraction
chemical_list: D003285:Contractile Proteins; D016207:Cytokines; D053583:Interleukin-1beta; D007473:Ion Channels; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha
keywords: P2X7 receptor; chronic inflammation; contractile-associated protein; odontogenic infection; preterm delivery