title: Combined delivery of PDGF-BB and BMP-6 for enhanced osteoblastic differentiation.
abstract: Natural microenvironment during bone tissue regeneration involves integration of multiple biological growth factors which regulate mitogenic activities and differentiation to induce bone repair. Among them platelet derived growth factor (PDGF-BB) and bone morphogenic protein-6 (BMP-6) are known to play a prominent role. The aim of this study was to investigate the benefits of combined delivery of PDGF-BB and BMP-6 on proliferation and osteoblastic differentiation of MC3T3-E1 preosteoblastic cells. PDGF-BB and BMP-6 were loaded in gelatin and poly (3-hydroxybutyric acid-co-3-hydroxyvaleric acid) particles, respectively. The carrier particles were then loaded into 3D chitosan matrix fabricated by freeze drying. The fast release of PDGF-BB during 7 days was accompanied by slower and prolonged release of BMP-6. The premising release of mitogenic factor PDGF-BB resulted in an increased MC3T3-E1 cell population seeded on chitosan scaffolds. Osteogenic markers of RunX2, Col 1, OPN were higher on chitosan scaffolds loaded with growth factors either individually or in combination. However, OCN expression and bone mineral formation were prominent on chitosan scaffolds incorporating PDGF-BB and BMP-6 as a combination.
authors.affiliation: Bioengineering Department, Hacettepe University, Beytepe, 06800, Ankara, Turkey.
authors.forename: T Tolga
authors.initials: TT
authors.lastname: Demirtaş
authors.affiliation: Chemical Engineering Department, Faculty of Engineering, Ankara University, 06100, Ankara, Turkey.
authors.forename: Eda
authors.initials: E
authors.lastname: Göz
authors.affiliation: Chemical Engineering Department, Faculty of Engineering, Ankara University, 06100, Ankara, Turkey. akarakecili@eng.ankara.edu.tr.
authors.forename: Ayşe
authors.initials: A
authors.lastname: Karakeçili
authors.affiliation: Bioengineering Department, Hacettepe University, Beytepe, 06800, Ankara, Turkey.|Chemical Engineering Department, Hacettepe University, Beytepe, 06800, Ankara, Turkey.
authors.forename: Menemşe
authors.initials: M
authors.lastname: Gümüşderelioğlu
mesh_terms: D016475:3T3 Cells; D000818:Animals; D000077214:Becaplermin; D055418:Bone Morphogenetic Protein 6 / Q000008:administration & dosage*; D002454:Cell Differentiation; D049109:Cell Proliferation; D051379:Mice; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000166:cytology*; D020574:Proto-Oncogene Proteins c-sis / Q000008:administration & dosage*; D054457:Tissue Scaffolds
chemical_list: D055418:Bone Morphogenetic Protein 6; D020574:Proto-Oncogene Proteins c-sis; D000077214:Becaplermin