title: Characteristics of esterified rice bran oil converted by enzymatic esterification.
abstract: In the present study, esterified rice bran oil (ERBO) was characterized using enzymatic esterification to improve stability, prevent acidification, enhance health-promoting biological activity and generate ω-3 PUFA-rich rice bran oil (RBO). Esterification reactions using RBO and ethanol were performed at 50°C under 200 bar with 3% lipozyme TL-IM (Thermomuces lanuginosa immobilized on silica gel) or RM-IM (Rhizomucor miehei immobilized on ion exchange resin) for 3 hr under supercritical CO2. The molar ratios of ethanol to RBO were 3, 6, 9 and 12, respectively. Total lipid contents and acid values decreased (maximum 83.75%),but γ-oryzanol content increased (maximum 41.33%) in esterified RBO (ERBO) prepared using TL-IM or RM-IM. In addition, DPPH radical scavenging activity of ERBO prepared by RM-IM atan ethanol to RBO molar ratio of 3 was 0.02 µg µl(-1), which was 63-fold higher than that of α-tocopherol (IC50 =1.25 µg µl(-1)). The anti-inflammatory effect of RM-IM 1:3 hydrolysate of RBO was verified showing its suppressive effect towards iNOS and Cox-2mRNA expression in a dose-dependent manner. Therefore, ERBO is a promising source of functional food, cosmetics and pharmaceuticals.
authors.forename: Jae-Suk
authors.initials: JS
authors.lastname: Choi
authors.forename: Myong-Kyun
authors.initials: MK
authors.lastname: Roh
authors.forename: Tae-Uk
authors.initials: TU
authors.lastname: Kim
authors.forename: Eun Jin
authors.initials: EJ
authors.lastname: Cheon
authors.forename: Woi-Sook
authors.initials: WS
authors.lastname: Moon
authors.forename: Mi-Ryung
authors.initials: MR
authors.lastname: Kim
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry; D001713:Biphenyl Compounds; D002460:Cell Line; D004951:Esterification; D008264:Macrophages / Q000187:drug effects; D051379:Mice; D010851:Picrates; D010938:Plant Oils / Q000737:chemistry* / Q000633:toxicity; D000073879:Rice Bran Oil
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D001713:Biphenyl Compounds; D010851:Picrates; D010938:Plant Oils; C004931:1,1-diphenyl-2-picrylhydrazyl; D000073879:Rice Bran Oil