title: Gold Nanoparticles Impair Foot-and-Mouth Disease Virus Replication.
abstract: In this study, we evaluated the antiviral activity of gold nanoparticles (AuNPs) against the foot-and-mouth disease virus (FMDV), that causes a contagious disease in cloven-hoofed animals. The anti-FMDV activity of AuNPs was assessed using plaque reduction assay. MTT assay was used for quantitatively measuring the cytopathic effect caused by the viral infection. The 50% cytotoxicity concentration of nanoparticles was measured and found to be 10.4 μg/ml. The virus yield reduction assay showed that AuNP have an approximately 4-fold virus titer reduction compared with controls. Plaque reduction assay showed that at non-cytotoxic concentrations, AuNPs do not show extracellular virucidal activity and inhibition of FMDV growth at the early stages of infection including attachment and penetration. Time-of-addition experiments revealed that AuNPs inhibited post-entry stages of viral replication concomitant with the onset of intracellular viral RNA synthesis; however, the mechanism of AuNPs against FMDV was unclear.
authors.forename: Solmaz
authors.initials: S
authors.lastname: Rafiei
authors.forename: Seyedeh Elham
authors.initials: SE
authors.lastname: Rezatofighi
authors.forename: Mohammad
authors.initials: M
authors.lastname: Roayaei Ardakani
authors.forename: Saadat
authors.initials: S
authors.lastname: Rastegarzadeh
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000737:chemistry / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D006224:Cricetinae; D029544:Foot-and-Mouth Disease Virus / Q000187:drug effects*; D006046:Gold / Q000737:chemistry / Q000494:pharmacology*; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000633:toxicity; D014779:Virus Replication / Q000187:drug effects*
chemical_list: D000998:Antiviral Agents; D006046:Gold