title: High-fat diet during pregnancy and lactation impairs the cholinergic anti-inflammatory pathway in the liver and white adipose tissue of mouse offspring.
abstract: Cholinergic anti-inflammatory pathway (CAP) prevents inflammatory cytokines production. The main was to evaluate the effect of maternal obesity on cholinergic pathway in the offspring. Female mice were subjected to either standard chow (SC) or high-fat diet (HFD) during pregnancy and the lactation period. After weaning, only male offspring from HFD dams (HFD-O) and from SC dams (SC-O) were fed the SC diet. Key proteins of the CAP were downregulated and serum TNF-α was elevated in the HFD-O mice. STAT3 and NF-κB activation in HFD-O mice ICV injected with nicotine (agonist) were lower than SC-O mice. Basal cholinesterase activity was upregulated in HFD-O mice in both investigated tissues. Lipopolysaccharide increased TNF-α and IL-1β expression in the liver and WAT of SC-O mice, but this effect was greater in HFD-O mice. In conclusion these changes exacerbated cytokine production in response to LPS and contributed to the reduced sensitivity of the CAP.
authors.affiliation: School of Applied Sciences, University of Campinas, Brazil.
authors.forename: Tanyara Baliani
authors.initials: TB
authors.lastname: Payolla
authors.affiliation: Biology Institute, University of Campinas, Brazil.
authors.forename: Simone Ferreira
authors.initials: SF
authors.lastname: Lemes
authors.affiliation: School of Applied Sciences, University of Campinas, Brazil.
authors.forename: Thaís
authors.initials: T
authors.lastname: de Fante
authors.affiliation: School of Applied Sciences, University of Campinas, Brazil.
authors.forename: Andressa
authors.initials: A
authors.lastname: Reginato
authors.affiliation: Department of Biosciences, Federal University of São Paulo, Brazil; Obesity and Comorbidities Research Center, State University of Campinas, Brazil.
authors.forename: Cristiano
authors.initials: C
authors.lastname: Mendes da Silva
authors.affiliation: School of Applied Sciences, University of Campinas, Brazil.
authors.forename: Thayana
authors.initials: T
authors.lastname: de Oliveira Micheletti
authors.affiliation: School of Applied Sciences, University of Campinas, Brazil.
authors.forename: Hosana Gomes
authors.initials: HG
authors.lastname: Rodrigues
authors.affiliation: School of Applied Sciences, University of Campinas, Brazil; Department of Biosciences, Federal University of São Paulo, Brazil; Obesity and Comorbidities Research Center, State University of Campinas, Brazil.
authors.forename: Adriana Souza
authors.initials: AS
authors.lastname: Torsoni
authors.affiliation: School of Applied Sciences, University of Campinas, Brazil; Department of Biosciences, Federal University of São Paulo, Brazil; Obesity and Comorbidities Research Center, State University of Campinas, Brazil.
authors.forename: Marciane
authors.initials: M
authors.lastname: Milanski
authors.affiliation: School of Applied Sciences, University of Campinas, Brazil; Department of Biosciences, Federal University of São Paulo, Brazil; Obesity and Comorbidities Research Center, State University of Campinas, Brazil. Electronic address: marcio.torsoni@fca.unicamp.br.
authors.forename: Marcio Alberto
authors.initials: MA
authors.lastname: Torsoni
mesh_terms: D052436:Adipose Tissue, White / Q000187:drug effects / Q000201:enzymology*; D000818:Animals; D002802:Cholinesterases / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D059305:Diet, High-Fat / Q000009:adverse effects*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D007774:Lactation / Q000187:drug effects* / Q000276:immunology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D039382:Maternal Nutritional Physiological Phenomena; D051379:Mice; D009765:Obesity / Q000201:enzymology / Q000209:etiology / Q000276:immunology*; D011247:Pregnancy / Q000187:drug effects*; D064569:alpha7 Nicotinic Acetylcholine Receptor / Q000378:metabolism*
chemical_list: D016207:Cytokines; D008070:Lipopolysaccharides; D064569:alpha7 Nicotinic Acetylcholine Receptor; D002802:Cholinesterases
keywords: Cholinergic anti-inflammatory pathway; High-fat diet; LPS; Maternal imprinting; Obese mice; α7nAChR