title: The levels of the GluN2A NMDA receptor subunit are modified in both the neonatal and adult rat brain by an early experience involving denial of maternal contact.
abstract: The composition of the N-methyl-d-aspartate receptor receptor in GluN2A/GluN2B subunits is important in determining its characteristics and its role in plasticity, a property of the brain which is known to be critically affected by early experiences. In the present work we employed an early experience model involving either receipt (RER) or denial (DER) of the expected reward of maternal contact within the context of learning by the pups of a T-maze on postnatal days (PND) 10-13. We investigated the effects of the RER and DER early experiences on GluN1, GluN2A and GluN2B levels in the prefrontal cortex (PFC), hippocampus and amygdala of the rat. We show that on PND13 the DER animals had lower GluN2A levels in the PFC. In adulthood DER males had higher GluN2A levels in the hippocampus, both under basal conditions and after exposure to a novel environment. The early experiences did not affect the response to the novelty. After exposure to a novel environment animals of all three groups (DER, RER, Control) responded with an increase in GluN2A levels in the brain areas examined. We did not detect any effects on GluN1 or GluN2B levels. The alterations in GluN2A levels observed in the DER animals could in part be responsible for their behavioral phenotype, described previously, which includes an increased susceptibility for the expression of depressive-like behavior.
authors.affiliation: Biology-Biochemistry Lab, Faculty of Nursing, School of Health Sciences, National and Kapodistrian, University of Athens, Greece.
authors.forename: V
authors.initials: V
authors.lastname: Manatos
authors.affiliation: Biology-Biochemistry Lab, Faculty of Nursing, School of Health Sciences, National and Kapodistrian, University of Athens, Greece.
authors.forename: F
authors.initials: F
authors.lastname: Stylianopoulou
authors.affiliation: Biology-Biochemistry Lab, Faculty of Nursing, School of Health Sciences, National and Kapodistrian, University of Athens, Greece. Electronic address: astam@nurs.uoa.gr.
authors.forename: A
authors.initials: A
authors.lastname: Stamatakis
mesh_terms: D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000378:metabolism*; D008297:Male; D008426:Maternal Deprivation; D021122:Protein Subunits / Q000378:metabolism; D017208:Rats, Wistar; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism*
chemical_list: D021122:Protein Subunits; D016194:Receptors, N-Methyl-D-Aspartate; C409466:NR1 NMDA receptor; C121001:NR2B NMDA receptor
keywords: Early life experience; NMDA; NR1; NR2A; NR2B; Novel environment