title: 18F-FDG PET imaging for identifying the dynamics of intestinal disease caused by SFTSV infection in a mouse model.
abstract: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging disease that causes fever, enteritis, thrombocytopenia, and leucopenia and can be fatal in up to 30% of cases. However, the mechanism of severe disease is not fully understood. Molecular imaging approaches, such as positron-emission tomography (PET), are functional in vivo imaging techniques that provide real-time dynamics of disease progression, assessments of pharmacokinetics, and diagnoses for disease progression. Molecular imaging also potentially provides useful approaches to explore the pathogenesis of viral infections. Thus, the purpose of this study was to image the pathological features of SFTSV infection in vivo by PET imaging. In a mouse model, we showed that 18F-FDG accumulations clearly identified the intestinal tract site as a pathological site. We also demonstrated that 18F-FDG PET imaging can assess disease progression and response to antiserum therapy within the same individual. This is the first report demonstrating a molecular imaging strategy for SFTSV infection. Our results provide potentially useful information for preclinical studies such as the elucidation of the mechanism of SFTSV infection in vivo and the assessment of drugs for SFTS treatment.
authors.affiliation: Department of Virology, Institute of Tropical Medicine, Nagasaki University, Sakamoto, Nagasaki, Japan.|Leading Graduate School Program, Nagasaki University, Sakamoto, Nagasaki, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Hayasaka
authors.affiliation: Department of Radioisotope Medicine, Atomic Bomb Diseases Institute, Nagasaki University, Sakamoto, Nagasaki, Japan.
authors.forename: Kodai
authors.initials: K
authors.lastname: Nishi
authors.affiliation: Department of Hygienic Chemistry, Graduate School of Biomedical Sciences, Nagasaki University, Bunkyo-machi, Nagasaki, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Fuchigami
authors.affiliation: Department of Pathology, Fujita Health University School of Medicine, Dengakugakubo, Kutsukake-cho, Toyoake, Aichi, Japan.
authors.forename: Kazuya
authors.initials: K
authors.lastname: Shiogama
authors.affiliation: Department of Pathology, Fujita Health University School of Medicine, Dengakugakubo, Kutsukake-cho, Toyoake, Aichi, Japan.
authors.forename: Takanori
authors.initials: T
authors.lastname: Onouchi
authors.affiliation: Department of Virology, Institute of Tropical Medicine, Nagasaki University, Sakamoto, Nagasaki, Japan.|Leading Graduate School Program, Nagasaki University, Sakamoto, Nagasaki, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Shimada
authors.affiliation: Department of Pathology, Fujita Health University School of Medicine, Dengakugakubo, Kutsukake-cho, Toyoake, Aichi, Japan.
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Tsutsumi
authors.affiliation: Department of Virology, Institute of Tropical Medicine, Nagasaki University, Sakamoto, Nagasaki, Japan.|Leading Graduate School Program, Nagasaki University, Sakamoto, Nagasaki, Japan.
authors.forename: Kouichi
authors.initials: K
authors.lastname: Morita
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology / Q000494:pharmacology; D002044:Bunyaviridae Infections / Q000175:diagnosis* / Q000000981:diagnostic imaging; D002522:Chlorocebus aethiops; D004195:Disease Models, Animal; D019788:Fluorodeoxyglucose F18; D054884:Host-Pathogen Interactions / Q000187:drug effects; D006801:Humans; D007410:Intestinal Diseases / Q000175:diagnosis* / Q000188:drug therapy / Q000821:virology; D051379:Mice; D064847:Multimodal Imaging / Q000379:methods; D016856:Phlebovirus / Q000254:growth & development* / Q000276:immunology / Q000502:physiology; D049268:Positron-Emission Tomography / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014057:Tomography, X-Ray Computed / Q000379:methods*; D014709:Vero Cells
chemical_list: D000914:Antibodies, Viral; D019788:Fluorodeoxyglucose F18
keywords: 18F-FDG PET imaging; Immune response; Immunity; Immunology and Microbiology Section; SFTSV; antiserum therapy; intestinal disorder; mouse model