title: miRNA 206 and miRNA 574-5p are highly expression in coronary artery disease.
abstract: Coronary artery disease (CAD) is the leading cause of human morbidity and mortality worldwide. Innovative diagnostic biomarkers are a pressing need for this disease. miRNAs profiling is an innovative method of identifying biomarkers for many diseases and could be proven as a powerful tool in the diagnosis and treatment of CAD. We performed miRNA microarray analysis from the plasma of three CAD patients and three healthy controls. Subsequently, we performed quantitative real-time PCR (qRT-PCR) analysis of miRNA expression in plasma of another 67 CAD patients and 67 healthy controls. We identified two miRNAs (miR-206 and miR-574-5p) that were significantly up-regulated in CAD patients as compared with healthy controls (P<0.05). The receiver operating characteristic (ROC) curves indicated these two miRNAs had great potential to provide sensitive and specific diagnostic value for CAD.
authors.affiliation: Ningbo Medical Center, Lihuili Hospital, Ningbo University, Ningbo, Zhejiang 315041, P.R. China.
authors.forename: Jianqing
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Ningbo Medical Center, Lihuili Hospital, Ningbo University, Ningbo, Zhejiang 315041, P.R. China.
authors.forename: Guofeng
authors.initials: G
authors.lastname: Shao
authors.affiliation: Ningbo Medical Center, Lihuili Hospital, Ningbo University, Ningbo, Zhejiang 315041, P.R. China Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, Ningbo, Zhejiang 315211, P.R. China.
authors.forename: Xiaoliang
authors.initials: X
authors.lastname: Chen
authors.affiliation: Ningbo Medical Center, Lihuili Hospital, Ningbo University, Ningbo, Zhejiang 315041, P.R. China.
authors.forename: Xi
authors.initials: X
authors.lastname: Yang
authors.affiliation: Ningbo Medical Center, Lihuili Hospital, Ningbo University, Ningbo, Zhejiang 315041, P.R. China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Huang
authors.affiliation: Ningbo Medical Center, Lihuili Hospital, Ningbo University, Ningbo, Zhejiang 315041, P.R. China Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, Ningbo, Zhejiang 315211, P.R. China.
authors.forename: Ping
authors.initials: P
authors.lastname: Peng
authors.affiliation: Ningbo Medical Center, Lihuili Hospital, Ningbo University, Ningbo, Zhejiang 315041, P.R. China Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, Ningbo, Zhejiang 315211, P.R. China.
authors.forename: Yanna
authors.initials: Y
authors.lastname: Ba
authors.affiliation: Diabetes Research Center, School of Medicine, Ningbo University, Ningbo, Zhejiang 315211, P.R. China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Diabetes Research Center, School of Medicine, Ningbo University, Ningbo, Zhejiang 315211, P.R. China.
authors.forename: Tashina
authors.initials: T
authors.lastname: Jehangir
authors.affiliation: Diabetes Research Center, School of Medicine, Ningbo University, Ningbo, Zhejiang 315211, P.R. China.
authors.forename: Shizhong
authors.initials: S
authors.lastname: Bu
authors.affiliation: Department of Pathology, Key Laboratory of Antibody Technique of Ministry of Health, Nanjing Medical University, Nanjing 210029, P.R. China liuns@njmu.edu.cn hjmpin@163.com.
authors.forename: Ningsheng
authors.initials: N
authors.lastname: Liu
authors.affiliation: Ningbo Medical Center, Lihuili Hospital, Ningbo University, Ningbo, Zhejiang 315041, P.R. China liuns@njmu.edu.cn hjmpin@163.com.
authors.forename: Jiangfang
authors.initials: J
authors.lastname: Lian
mesh_terms: D015415:Biomarkers / Q000097:blood; D003324:Coronary Artery Disease / Q000097:blood*; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods
chemical_list: D015415:Biomarkers; C546087:MIRN206 microRNA, human; C558811:MIRN574 microRNA, human; D035683:MicroRNAs
keywords: biomarker; coronary artery disease; miRNA; plasma