title: Simultaneous sampling of tissue oxygenation and oxygen consumption in skeletal muscle.
abstract: Under physiologic conditions, microvascular oxygen delivery appears to be well matched to oxygen consumption in respiring tissues. We present a technique to measure interstitial oxygen tension (PISFO2) and oxygen consumption (VO2) under steady-state conditions, as well as during the transitions from rest to activity and back. Phosphorescence Quenching Microscopy (PQM) was employed with pneumatic compression cycling to achieve 1 to 10 Hz sampling rates of interstitial PO2 and simultaneous recurrent sampling of VO2 (3/min) in the exteriorized rat spinotrapezius muscle. The compression pressure was optimized to 120-130 mmHg without adverse effect on the tissue preparation. A cycle of 5s compression followed by 15s recovery yielded a resting VO2 of 0.98 ± 0.03 ml O2/100 cm(3)min while preserving microvascular oxygen delivery. The measurement system was then used to assess VO2 dependence on PISFO2 at rest and further tested under conditions of isometric muscle contraction to demonstrate a robust ability to monitor the on-kinetics of tissue respiration and the compensatory changes in PISFO2 during contraction and recovery. The temporal and spatial resolution of this approach is well suited to studies seeking to characterize microvascular oxygen supply and demand in thin tissues.
authors.affiliation: Department of Physiology and Biophysics, Medical College of Virginia Campus, Virginia Commonwealth University, Richmond, VA 23298, USA.
authors.forename: William H
authors.initials: WH
authors.lastname: Nugent
authors.affiliation: Department of Physiology and Biophysics, Medical College of Virginia Campus, Virginia Commonwealth University, Richmond, VA 23298, USA.
authors.forename: Bjorn K
authors.initials: BK
authors.lastname: Song
authors.affiliation: Department of Physiology and Biophysics, Medical College of Virginia Campus, Virginia Commonwealth University, Richmond, VA 23298, USA. Electronic address: pittman@vcu.edu.
authors.forename: Roland N
authors.initials: RN
authors.lastname: Pittman
authors.affiliation: Department of Physiology and Biophysics, Medical College of Virginia Campus, Virginia Commonwealth University, Richmond, VA 23298, USA.
authors.forename: Aleksander S
authors.initials: AS
authors.lastname: Golub
mesh_terms: D000818:Animals; D001783:Blood Flow Velocity; D066298:In Vitro Techniques; D000069416:Intravital Microscopy; D007537:Isometric Contraction; D007700:Kinetics; D008297:Male; D008833:Microcirculation; D008856:Microscopy, Fluorescence; D055806:Microvessels / Q000502:physiology; D010100:Oxygen / Q000097:blood / Q000378:metabolism*; D010101:Oxygen Consumption; D011312:Pressure; D017207:Rats, Sprague-Dawley; D012039:Regional Blood Flow; D012146:Rest; D064171:Superficial Back Muscles / Q000098:blood supply / Q000378:metabolism*
chemical_list: D010100:Oxygen
keywords: Microvasculature; Muscle stimulation; Oxygen consumption (VO(2)); Oxygen measurement; Phosphorescence Quenching Microscopy (PQM); Rat spinotrapezius muscle; Skeletal muscle; Tissue compression