title: Comprehensive analysis of serum metabolites in gestational diabetes mellitus by UPLC/Q-TOF-MS.
abstract: Gestational diabetes mellitus (GDM) refers to the first sign or onset of diabetes mellitus during pregnancy rather than progestation. In recent decades, more and more research has focused on the etiology and pathogenesis of GDM in order to further understand GDM progress and recovery. Using an advanced metabolomics platform based on ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF-MS), we explored the changes in serum metabolites between women with GDM and healthy controls during and after pregnancy. Some significant differences were discovered using multivariate analysis including partial least-squares discriminant analysis (PLS-DA) and orthogonal PLS-DA (OPLS-DA). The dysregulated metabolites were further compared and verified in several databases to understand how these compounds might function as potential biomarkers. Analyses of the metabolic pathways associated with these potential biomarkers were subsequently explored. A total of 35 metabolites were identified, contributing to GDM progress to some extent. The identified biomarkers were involved in some important metabolic pathways including glycine, serine, and threonine metabolism; steroid hormone biosynthesis; tyrosine metabolism; glycerophospholipid metabolism; and fatty acid metabolism. The above mentioned metabolic pathways mainly participate in three major metabolic cycles in humans, including lipid metabolism, carbohydrate metabolism, and amino acid metabolism. In this pilot study, the valuable comprehensive analysis gave us further insight into the etiology and pathophysiology of GDM, which might benefit the feasibility of a rapid, accurate diagnosis and reasonable treatment as soon as possible but also prevent GDM and its related short- and long-term complications.
authors.affiliation: Pi County People's Hospital, Chengdu, Sichuan, 611730, China.
authors.forename: Tianhu
authors.initials: T
authors.lastname: Liu
authors.affiliation: Department of Obstetrics and Gynecology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China.
authors.forename: Jiaxun
authors.initials: J
authors.lastname: Li
authors.affiliation: Pi County People's Hospital, Chengdu, Sichuan, 611730, China.
authors.forename: Fengcheng
authors.initials: F
authors.lastname: Xu
authors.affiliation: Department of Obstetrics and Gynecology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China.
authors.forename: Mengni
authors.initials: M
authors.lastname: Wang
authors.affiliation: Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, China.
authors.forename: Shijia
authors.initials: S
authors.lastname: Ding
authors.affiliation: Department of Obstetrics and Gynecology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China. xhb098567@163.com.
authors.forename: Hongbing
authors.initials: H
authors.lastname: Xu
authors.affiliation: Department of Endocrinology &The First Branch, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China. dongfannie@163.com.
authors.forename: Fang
authors.initials: F
authors.lastname: Dong
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood*; D016022:Case-Control Studies; D002853:Chromatography, Liquid / Q000379:methods*; D016640:Diabetes, Gestational / Q000097:blood*; D016002:Discriminant Analysis; D005260:Female; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D053858:Metabolic Networks and Pathways; D055432:Metabolomics / Q000379:methods; D015999:Multivariate Analysis; D010865:Pilot Projects; D011247:Pregnancy; D044967:Serum / Q000378:metabolism
chemical_list: D015415:Biomarkers
keywords: Biomarkers; Gestational diabetes mellitus; Metabolomics; Pathogenesis; UPLC/Q-TOF-MS