title: The Activation of Nrf2 and Its Downstream Regulated Genes Mediates the Antioxidative Activities of Xueshuan Xinmaining Tablet in Human Umbilical Vein Endothelial Cells.
abstract: Epidemiological studies have verified the critical role that antioxidative stress plays in protecting vascular endothelial cells. The aims of the present study were to investigate the antioxidative activities and differential regulation of nuclear erythroid-related factor 2- (Nrf2-) mediated gene expression by Xueshuan Xinmaining Tablet (XXT), a traditional Chinese medicine with the effect of treating cardiovascular diseases. The antioxidative activities of XXT were investigated using quantitative real-time PCR (qPCR), a PCR array, and western blotting. Our results indicated that XXT exhibited potent antioxidative activities by suppressing the levels of hydrogen peroxide- (H2O2-) induced reactive oxygen species (ROS) in human umbilical vein endothelial cells (HUVECs). We were also conscious of strong Nrf2-mediated antioxidant induction. XXT enhanced the expressions of Keap1, Nrf2, and Nrf2-mediated genes, such as glutamate-cysteine ligase modifier subunit (GCLM), NAD(P)H: quinine oxidoreductase 1 (NQO1), heme oxygenase 1 (HMOX1), and glutathione peroxidase (GPX) in HUVECs. In summary, XXT strongly activated Nrf2 and its downstream regulated genes, which may contribute to the antioxidative and vascular endothelial cell protective activities of XXT.
authors.affiliation: Department of Pathogeny Biology, Basic Medical College, Jilin University, Changchun 130021, China.
authors.forename: Lingxin
authors.initials: L
authors.lastname: Xiong
authors.affiliation: Department of Pathogeny Biology, Basic Medical College, Jilin University, Changchun 130021, China.
authors.forename: Jingshu
authors.initials: J
authors.lastname: Xie
authors.affiliation: Department of Pathogeny Biology, Basic Medical College, Jilin University, Changchun 130021, China.
authors.forename: Chenxue
authors.initials: C
authors.lastname: Song
authors.affiliation: Department of Pharmaceutical Sciences, Jilin University, Changchun 130021, China.
authors.forename: Jinping
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Pathogeny Biology, Basic Medical College, Jilin University, Changchun 130021, China.
authors.forename: Jingtong
authors.initials: J
authors.lastname: Zheng
authors.affiliation: Department of Pharmaceutical Sciences, Jilin University, Changchun 130021, China.
authors.forename: Chuangui
authors.initials: C
authors.lastname: Liu
authors.affiliation: Department of Pathogeny Biology, Basic Medical College, Jilin University, Changchun 130021, China.
authors.forename: Xiaotian
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Pharmaceutical Sciences, Jilin University, Changchun 130021, China.
authors.forename: Pingya
authors.initials: P
authors.lastname: Li
authors.affiliation: Department of Pathogeny Biology, Basic Medical College, Jilin University, Changchun 130021, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Wang