title: MicroRNA-181c inhibits glioblastoma cell invasion, migration and mesenchymal transition by targeting TGF-β pathway.
abstract: MicroRNAs (miRNAs) are small non-coding RNAs frequently dysregulated in human malignancies. In this study, we found that miR-181c was down-regulated both in glioblastoma tissues and cell lines. We also annotated 566 TCGA miRNA expression profiles and found that patients with high microRNA-181c (miR-181c)-expressing tumors had significantly longer OS and PFS. Overexpression of miR-181c evidently inhibited glioblastoma cell line T98G migration and invasion. Further, the expression of E-cadherin was significantly upregulated and that of N-cadherin and vimentin was significantly down-regulated. We also found that miR-181c overexpression inhibited TGF-β signaling by down-regulating TGFBR1, TGFBR2 and TGFBRAP1 expression. Overall, our study found that miR-181c plays a key role in glioblastoma cell invasion, migration and mesenchymal transition suggesting potential therapeutic applications.
authors.affiliation: Department of Neurosurgery, Beijing Sanbo Brain Hospital, Capital Medical University, China.
authors.forename: Xin
authors.initials: X
authors.lastname: He
authors.affiliation: Department of Neurosurgery, Beijing Sanbo Brain Hospital, Capital Medical University, China.
authors.forename: Zengjin
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Neurosurgery, Beijing Sanbo Brain Hospital, Capital Medical University, China.
authors.forename: Yutao
authors.initials: Y
authors.lastname: Peng
authors.affiliation: Department of Neurosurgery, Beijing Sanbo Brain Hospital, Capital Medical University, China. Electronic address: chunjiangyu1955@126.com.
authors.forename: Chunjiang
authors.initials: C
authors.lastname: Yu
mesh_terms: D014408:Biomarkers, Tumor / Q000378:metabolism; D001932:Brain Neoplasms / Q000378:metabolism* / Q000401:mortality*; D045744:Cell Line, Tumor; D002465:Cell Movement; D002681:China / Q000453:epidemiology; D058750:Epithelial-Mesenchymal Transition; D005909:Glioblastoma / Q000378:metabolism* / Q000401:mortality*; D006801:Humans; D035683:MicroRNAs / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D015995:Prevalence; D012307:Risk Factors; D015398:Signal Transduction; D015996:Survival Rate; D016212:Transforming Growth Factor beta / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; C540193:MIrn181 microRNA, human; D035683:MicroRNAs; D016212:Transforming Growth Factor beta
keywords: EMT; Glioblastoma; MicroRNA-181c; TGF-β