title: [Effect of Zhibai Dihuang Decoction on Expressions of TRPV1 and TRPV5 in Spermatogenic Cells of UU-infected Rats].
abstract: OBJECTIVE
  To observe the effect of Zhibai Dihuang Decoction (ZDD) on mRNA and protein expressions of transient receptor potential family vanilloid subtype 1 (TRPV1) and transient receptor potential family vanilloid subtype 5 (TRPV5) in Ureaplasma urealyticum (UU)-infected rat semens and spermatogenic cells, and to explore the pathomechanism of UU-infected infertility and the intervention of ZDD.
  
  
  METHODS
  Totally 45 were randomly selected from 60 4-5 months old SD rats. UU testicular infected animal models were set up after bladder inoculation of UU suspension. The remaining 15 rats were simultaneously injected with normal saline as a normal control group. After a successful modeling, UU infected model rats were randomly divided into the model group, the azithromycin group, and the ZDD group, 15 in each group. Rats in the ZDD group were administered with ZDD at the daily dose of 1 g/kg by gastrogavage. Rats in the azithromycin group were administered with azithromycin suspension at the daily dose of 0. 105 mg/kg by gastrogavage. Equal volume of normal saline was administered to rats in the normal control group and the model group. All medication was performed once daily for 21 successive days. Testes and epididymis were extracted after rats were killed and UU positive rates were compared among all groups. Sperm cells were separated using a mechanical separation technique. Sperm motility parameters were detected using color sperm motion detection system. mRNA and protein expressions of TRPV1 and TRPV5 in spermatogenic cells were determined by real-time quantitative PCR and Western blot.
  
  
  RESULTS
  The UU positive rate was obviously higher in the model group than in the normal control group [(86.7% (13/15 cases) vs. 0] P < 0.05). It was lower in the ZDD group [33.3% (5/15 cases)] and the azithromycin group [26.7% (4/15 cases)] than in the model group (P < 0.05). Compared with the normal control group, class A and B sperms were reduced, the linear velocity and the average velocity were significantly lowered, mRNA and protein expressions of TRPV1 and TRPV5 in spermated genic cells significantly decreased in the model group (P < 0.05). Compared with the model group, class A and B sperms were increased, linear and curve velocities and the average velocity were significantly elevated, mRNA and protein expressions of TRPV1 and TRPV5 significantly increased in the ZDD group and the azithromycin group (P < 0.05, P < 0.01). Compared with azithromycin group, class A and B sperms were increased, the linear velocity and the average velocity were significantly elevated, mRNA and protein expressions of TRPV1 and TRPV5 significantly increased in the ZDD group (P < 0.05, P < 0.01).
  
  
  CONCLUSIONS
  ZDD could fight against UU infection and elevate semen quality, which might be associated with up-regulating mRNA and protein expressions of TRPV1 and TRPV5 in spermatogenic cells.
authors.forename: Qing-hu
authors.initials: QH
authors.lastname: He
authors.forename: Xuan-zuo
authors.initials: XZ
authors.lastname: Guo
authors.forename: Jun-hua
authors.initials: JH
authors.lastname: Guo
authors.forename: Ying-qiu
authors.initials: YQ
authors.lastname: Li
authors.forename: Chao-sheng
authors.initials: CS
authors.lastname: Liu
mesh_terms: D000818:Animals; D015220:Calcium Channels / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D007246:Infertility; D008297:Male; D012333:RNA, Messenger; D051381:Rats; D017207:Rats, Sprague-Dawley; D055101:Semen Analysis; D013081:Sperm Motility; D013094:Spermatozoa; D050916:TRPV Cation Channels / Q000378:metabolism*; D013737:Testis; D016869:Ureaplasma Infections; D016990:Ureaplasma urealyticum
chemical_list: D015220:Calcium Channels; D004365:Drugs, Chinese Herbal; D012333:RNA, Messenger; D050916:TRPV Cation Channels; C494484:TRPV1 receptor; C492241:TRPV5 protein, rat; C540472:zhibai dihuang