title: Estrogen receptor β controls MMP-19 expression in mouse ovaries during ovulation.
abstract: Estrogen receptor beta (ERβ/ESR2) has a central role in mouse ovaries, as ERβ knockout (BERKO) mice are subfertile due to an increase in fibrosis around the maturing follicle and a decrease in blood supply. This has a consequence that these follicles rarely rupture to release the mature oocyte. Matrix metalloproteinases (MMPs) are modulators of the extracellular matrix, and the expression of one specific MMP, MMP-19, is normally increased in granulosa cells during their maturation until ovulation. In this study, we demonstrate that MMP-19 levels are downregulated in BERKO mouse ovaries. Using human MCF-7 cells that overexpress ERβ, we could identify MMP-19 to be a transcriptional target of ligand-bound activated ERβ acting on a specificity protein-1 binding site. These data provide a molecular explanation for the observed follicle rupture defect that contributes to the subfertility of female BERKO mice.
authors.affiliation: Department of Biosciences and NutritionKarolinska Institutet, SE-14183 Huddinge, SwedenCenter for Nuclear Receptors and SignalingUniversity of Houston, Houston, Texas 77204-5056, USA.
authors.forename: Ivan
authors.initials: I
authors.lastname: Nalvarte
authors.affiliation: Department of Biosciences and NutritionKarolinska Institutet, SE-14183 Huddinge, SwedenCenter for Nuclear Receptors and SignalingUniversity of Houston, Houston, Texas 77204-5056, USA.
authors.forename: Virpi
authors.initials: V
authors.lastname: Töhönen
authors.affiliation: Department of Biosciences and NutritionKarolinska Institutet, SE-14183 Huddinge, SwedenCenter for Nuclear Receptors and SignalingUniversity of Houston, Houston, Texas 77204-5056, USA.
authors.forename: Maria
authors.initials: M
authors.lastname: Lindeberg
authors.affiliation: Department of Biosciences and NutritionKarolinska Institutet, SE-14183 Huddinge, SwedenCenter for Nuclear Receptors and SignalingUniversity of Houston, Houston, Texas 77204-5056, USA.
authors.forename: Mukesh
authors.initials: M
authors.lastname: Varshney
authors.affiliation: Department of Biosciences and NutritionKarolinska Institutet, SE-14183 Huddinge, SwedenCenter for Nuclear Receptors and SignalingUniversity of Houston, Houston, Texas 77204-5056, USA Department of Biosciences and NutritionKarolinska Institutet, SE-14183 Huddinge, SwedenCenter for Nuclear Receptors and SignalingUniversity of Houston, Houston, Texas 77204-5056, USA.
authors.forename: Jan-Åke
authors.initials: JÅ
authors.lastname: Gustafsson
authors.affiliation: Department of Biosciences and NutritionKarolinska Institutet, SE-14183 Huddinge, SwedenCenter for Nuclear Receptors and SignalingUniversity of Houston, Houston, Texas 77204-5056, USA jose.inzunza@ki.se.
authors.forename: José
authors.initials: J
authors.lastname: Inzunza
mesh_terms: D000818:Animals; D006063:Chorionic Gonadotropin; D015536:Down-Regulation; D047628:Estrogen Receptor alpha / Q000378:metabolism; D047629:Estrogen Receptor beta / Q000378:metabolism*; D005260:Female; D006801:Humans; D061986:MCF-7 Cells; D053505:Matrix Metalloproteinases, Secreted / Q000235:genetics / Q000378:metabolism*; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D010053:Ovary / Q000378:metabolism*; D010060:Ovulation; D016329:Sp1 Transcription Factor / Q000378:metabolism
chemical_list: D006063:Chorionic Gonadotropin; D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D016329:Sp1 Transcription Factor; D053505:Matrix Metalloproteinases, Secreted; C108362:matrix metalloproteinase 19