title: Molecular Background of Estrogen Receptor Gene Expression in Endometriotic Cells.
abstract: The molecular background of estrogen receptor (ER) expression is important to understand the pathophysiology of the high estrogen environment in endometriosis. However, the molecular details have not been fully understood. The objective of this study is to evaluate the molecular background of ERα and ERβ messenger RNA (mRNA) expression in endometriotic cells. The following summarizes our observations: (1) ERα mRNA expression in endometriotic cells was estimated to be approximately one-tenth of that in endometrial cells. (2) Three mRNAs, which include 3 different 5'-untranslated exons tagged to an open reading frame of wild-type ERα, were detected. (3) Expression of ERβ mRNA depends mostly on 0N promoter and includes 2 open reading frames: one for a wild-type ERβ1 and another for a splice variant ERβ2. (4) Expression of ERβ1 mRNA was approximately 40-fold higher than that in endometrial cells. (5) Expression of ERβ2 mRNA was almost at a comparable level of the ERβ1. 9 (6) ERα and ERβ mRNAs are equivalently expressed in endometriotic cells. These observations show the molecular background of ER mRNA expression in endometriotic cells and provide a clue to further understanding the estrogen-dependent pathophysiology leading to clinical application in endometriosis.
authors.affiliation: Department of Obstetrics and Gynecology, Tottori University Faculty of Medicine, Yonago, Japan 1mizawa@grape.med.tottori-u.ac.jp.
authors.forename: Masao
authors.initials: M
authors.lastname: Izawa
authors.affiliation: Department of Obstetrics and Gynecology, Tottori University Faculty of Medicine, Yonago, Japan.
authors.forename: Fuminori
authors.initials: F
authors.lastname: Taniguchi
authors.affiliation: Department of Obstetrics and Gynecology, Tottori University Faculty of Medicine, Yonago, Japan.
authors.forename: Tasuku
authors.initials: T
authors.lastname: Harada
mesh_terms: D004715:Endometriosis / Q000378:metabolism*; D004717:Endometrium / Q000378:metabolism*; D047628:Estrogen Receptor alpha / Q000235:genetics* / Q000378:metabolism; D047629:Estrogen Receptor beta / Q000235:genetics* / Q000378:metabolism; D005091:Exons; D005260:Female; D015870:Gene Expression; D006801:Humans; D016366:Open Reading Frames; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D012333:RNA, Messenger
keywords: endometriosis; estrogen receptor α; estrogen receptor β; mRNA expression