title: Advances in mechanisms and modifications for rendering yeast thermotolerance.
abstract: Thermotolerant Saccharomyces cerevisiae is widely regarded as an attractive strain with which to accomplish the coupling of enzyme saccharification, ethanol fermentation and ethanol distillation in non-grain fuel bioethanol fermentation systems, and it has many advantages for increasing the ethanol yield and reducing production costs. This review provided an overview of the yeast heat-resistant mechanisms from six aspects, including gene expression responses, heat shock proteins, trehalose, ATPase, the ubiquitin-proteasome pathway and heat-induced antioxidant defenses. Innovative methods, such as random and rational strategies for improving yeast thermotolerance, were further discussed, and several special cases were provided. To rationally engineer thermotolerance in yeast, the advances in employing heat-resistant mechanisms of thermophiles were particularly discussed. By designing and constructing heat-resistant devices consists of heat-resistant parts from thermophiles to yeast, a superior thermotolerance of S. cerevisiae has been achieved, providing a new system with important applications for research regarding the improvement of the robustness of microbes.
authors.affiliation: School of Life Science, Beijing Institute of Technology, 5th Building, Zhongguancun South Street, Beijing 100081, China.
authors.forename: Liman
authors.initials: L
authors.lastname: Gao
authors.affiliation: School of Life Science, Beijing Institute of Technology, 5th Building, Zhongguancun South Street, Beijing 100081, China.
authors.forename: Yueqin
authors.initials: Y
authors.lastname: Liu
authors.affiliation: School of Life Science, Beijing Institute of Technology, 5th Building, Zhongguancun South Street, Beijing 100081, China.
authors.forename: Hun
authors.initials: H
authors.lastname: Sun
authors.affiliation: School of Life Science, Beijing Institute of Technology, 5th Building, Zhongguancun South Street, Beijing 100081, China.
authors.forename: Chun
authors.initials: C
authors.lastname: Li
authors.affiliation: School of Chemical Engineering and the Environment, Beijing Institute of Technology, 5th Building, Zhongguancun South Street, Beijing 100081, China.
authors.forename: Zhiping
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: School of Life Science, Beijing Institute of Technology, 5th Building, Zhongguancun South Street, Beijing 100081, China. Electronic address: gyliu@bit.edu.cn.
authors.forename: Guiyan
authors.initials: G
authors.lastname: Liu
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism; D000975:Antioxidants / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D006358:Hot Temperature; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D000071436:Thermotolerance / Q000235:genetics; D014199:Trehalose / Q000378:metabolism; D025801:Ubiquitin / Q000378:metabolism
chemical_list: D000975:Antioxidants; D006360:Heat-Shock Proteins; D029701:Saccharomyces cerevisiae Proteins; D025801:Ubiquitin; D014199:Trehalose; D046988:Proteasome Endopeptidase Complex; D000251:Adenosine Triphosphatases
keywords: Heat-resistant devices; Heat-resistant modification; Non-gain fuel bioethanol; Saccharomyces cerevisiae; Thermophiles; Thermotolerant mechanism