title: Organ Culture as a Model System for Studies on Enterotoxin Interactions with the Intestinal Epithelium.
abstract: Studies on bacterial enterotoxin-epithelium interactions require model systems capable of mimicking the events occurring at the molecular and cellular levels during intoxication. In this chapter, we describe organ culture as an often neglected alternative to whole-animal experiments or enterocyte-like cell lines. Like cell culture, organ culture is versatile and suitable for studying rapidly occurring events, such as enterotoxin binding and uptake. In addition, it is advantageous in offering an epithelium with more authentic permeability/barrier properties than any cell line, as well as a subepithelial lamina propria, harboring the immune cells of the gut mucosa.
authors.affiliation: Faculty of Health Sciences, Department of Cellular and Molecular Medicine, The Panum Institute, University of Copenhagen, Copenhagen N, Denmark.
authors.forename: Ulver Spangsberg
authors.initials: US
authors.lastname: Lorenzen
authors.affiliation: Faculty of Health Sciences, Department of Cellular and Molecular Medicine, The Panum Institute, University of Copenhagen, Copenhagen N, Denmark.
authors.forename: Gert H
authors.initials: GH
authors.lastname: Hansen
authors.affiliation: Faculty of Health Sciences, Department of Cellular and Molecular Medicine, The Panum Institute, University of Copenhagen, Copenhagen N, Denmark. midan@sund.ku.dk.
authors.forename: E Michael
authors.initials: EM
authors.lastname: Danielsen
mesh_terms: D000818:Animals; D004768:Enterotoxins / Q000276:immunology* / Q000378:metabolism*; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000276:immunology* / Q000378:metabolism*; D009924:Organ Culture Techniques
chemical_list: D004768:Enterotoxins
keywords: Brush border; Enterocytes; Enterotoxins; Mucosal explants; Organ culture; Small intestine