title: Inducing Humoral Immune Responses Against Regulatory T Cells by Foxp3-Fc(IgG) Fusion Protein.
abstract: The existence of a developed network of suppressory factors and cells against an immune response in different cancers has been proven; regulatory T cells are a typical issue. Therefore their depletion, elimination, or suppression has been assessed in different research studies that were not entirely successful. By applying an improved vaccine against regulatory T cells, we have evaluated the B cell response elicited by the vaccine in an experimental design. A previously described DNA vaccine and recombinant protein of Foxp3-Fc fusion were produced and used in the vaccination regimen. DNA construct and respective protein were injected into C57BL/6 mice. After 2 weeks, serum levels of IgG antibody and its subtypes against Foxp3 were investigated by ELISA. To produce recombinant Foxp3 for ELISA antigen coating, pET24a-Foxp3 vector was transformed into Escherichia coli strain BL21 as host cells. Afterward, protein was expressed and then purified using Ni-NTA agarose. SDS-PAGE and Western blot analysis were carried out to confirm protein expression. The expression analysis of Foxp3 was confirmed by SDS-PAGE followed by Western blot analysis. FOXP3-Fc DNA vaccine/fusion protein vaccination regimen could induce T helper-dependent humoral responses. Due to the effectiveness of Foxp3-Fc(IgG) in inducing humoral responses, it would be expected to be useful in developing vaccines in tumor therapies for the removal of regulatory T cells as a strategy for increasing the efficiency of other means of immunotherapy.
authors.affiliation: 1 Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, University of Medical Sciences , Tabriz, Iran .
authors.forename: Neda Mousavi
authors.initials: NM
authors.lastname: Niri
authors.affiliation: 2 Department of Immunology, Faculty of Medicine, Tehran University of Medical Sciences , Tehran, Iran .
authors.forename: Jamshid
authors.initials: J
authors.lastname: Hadjati
authors.affiliation: 3 Department of Hepatitis and HIV, Pasteur Institute of Iran , Tehran, Iran .
authors.forename: Mahdi
authors.initials: M
authors.lastname: Sadat
authors.affiliation: 3 Department of Hepatitis and HIV, Pasteur Institute of Iran , Tehran, Iran .
authors.forename: Arash
authors.initials: A
authors.lastname: Memarnejadian
authors.affiliation: 3 Department of Hepatitis and HIV, Pasteur Institute of Iran , Tehran, Iran .
authors.forename: Mohammadreza
authors.initials: M
authors.lastname: Aghasadeghi
authors.affiliation: 1 Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, University of Medical Sciences , Tabriz, Iran .
authors.forename: Abolfazl
authors.initials: A
authors.lastname: Akbarzadeh
authors.affiliation: 1 Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, University of Medical Sciences , Tabriz, Iran .|4 Department of Clinical Biochemistry, University of Medical Sciences , Tabriz, Iran .
authors.forename: Nosratollah
authors.initials: N
authors.lastname: Zarghami
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D019496:Cancer Vaccines / Q000008:administration & dosage* / Q000096:biosynthesis / Q000276:immunology; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005260:Female; D051858:Forkhead Transcription Factors / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology; D015870:Gene Expression; D056724:Immunity, Humoral / Q000187:drug effects*; D007141:Immunoglobulin Fc Fragments / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology; D007074:Immunoglobulin G / Q000096:biosynthesis; D008212:Lymphocyte Depletion; D051379:Mice; D008810:Mice, Inbred C57BL; D010957:Plasmids / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology; D011993:Recombinant Fusion Proteins / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology; D006377:T-Lymphocytes, Helper-Inducer / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D014611:Vaccination; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000096:biosynthesis / Q000276:immunology
chemical_list: D019496:Cancer Vaccines; D051858:Forkhead Transcription Factors; C498833:Foxp3 protein, mouse; D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; D011993:Recombinant Fusion Proteins; D019444:Vaccines, DNA