title: Steroid hormones related to 11beta-hydroxysteroid dehydrogenase type 1 in treated obesity.
abstract: The local concentration of glucocorticoids is intensively regulated by 11beta-hydroxysteroid dehydrogenase type 1 (11beta-HSD 1). Human 11beta-HSD 1 also reversibly catalyzes the inter-conversion of 7alpha-hydroxy- and 7beta-hydroxy-dehydroepiandrosterone (DHEA) into 7-oxo-DHEA. The cohort of 282 obese adolescents, 154 girls (median age 15.31 years, range 14.17-16.68 years) and 128 boys (median age 14.95 years, range 13.87-16.16 years), BMI (Body Mass Index) >90th percentile was examined. In samples collected before and after one month of reductive diet therapy, circulating levels of steroids were analyzed by liquid chromatography-tandem mass spectrometry and radioimmunoassay methods. The model of the treatment efficacy prediction was calculated. A significant reduction in circulating levels of cortisone, E2 and increased levels of 7beta-hydroxy-DHEA after the reductive treatment was observed. Levels of cortisol, DHEA, DHT sustained without any significant change. The predictive Orthogonal Projections to Latent Structures (OPLS) model explained 20.1 % of variability of BMI, z-score change by the basal levels of 7alpha-hydroxy-DHEA, DHEA, cortisol and E2 as the strongest predictors. Reduced levels of circulating cortisone and reduced ratios of oxygenated/reduced metabolites reflect increased reductase activity of 11beta-HSD 1 with reduced BMI, z-score. We hypothesize whether these changes can be attributed to the altered activity of 11beta-HSD 1 in the liver.
authors.affiliation: Department of Steroids and Proteofactors, Institute of Endocrinology, Prague, Czech Republic. lmacova@endo.cz.
authors.forename: L
authors.initials: L
authors.lastname: Máčová
authors.forename: L
authors.initials: L
authors.lastname: Sosvorová
authors.forename: J
authors.initials: J
authors.lastname: Vítků
authors.forename: M
authors.initials: M
authors.lastname: Bičíková
authors.forename: M
authors.initials: M
authors.lastname: Hill
authors.forename: H
authors.initials: H
authors.lastname: Zamrazilová
authors.forename: B
authors.initials: B
authors.lastname: Sedláčková
authors.forename: L
authors.initials: L
authors.lastname: Stárka
mesh_terms: D043205:11-beta-Hydroxysteroid Dehydrogenase Type 1 / Q000097:blood*; D000293:Adolescent; D015415:Biomarkers / Q000097:blood; D015331:Cohort Studies; D003687:Dehydroepiandrosterone / Q000097:blood*; D004038:Diet, Reducing / Q000639:trends*; D005260:Female; D006801:Humans; D008099:Liver / Q000378:metabolism; D008297:Male; D009765:Obesity / Q000097:blood* / Q000628:therapy*; D016896:Treatment Outcome
chemical_list: D015415:Biomarkers; D003687:Dehydroepiandrosterone; D043205:11-beta-Hydroxysteroid Dehydrogenase Type 1