title: Circulating microRNA 132-3p and 324-3p Profiles in Patients after Acute Aneurysmal Subarachnoid Hemorrhage.
abstract: BACKGROUND
  Aneurysmal subarachnoid hemorrhage (SAH) is a highly morbid and fatal condition with high rate of cognitive impairment and negative impact in quality of life among survivors. Delayed cerebral infarction (DCI) is one the major factors for these negative outcomes. In this study we compared the circulating microRNA profiles of SAH patients and healthy individuals, and the circulating microRNA profiles of SAH patients with and without DCI.
  
  
  METHODS
  Peripheral blood samples on Day 7 after the onset of SAH were subjected to microarray analysis with Affymetrix miRNA 3.0 array and quantitative PCR analysis. SAH patients with (N = 20) and without DCI (N = 20) and Healthy controls (N = 20) were included for analyses.
  
  
  RESULTS
  We demonstrated that 99 miRNAs were found to be dysregulated in the SAH patient group with DCI. 81 miRNAs were upregulated and 18 were downregulated. Findings from KEGG pathway analysis showed that miRNAs and target genes for axon guidance and TGF-beta signaling were involved, implying that the resulted differential miRNA expression pattern reflect the results of SAH instead of etiology of the disease. miR-132-3p and miR-324-3p showed distinctive upregulations in qPCR [miR-132: 9.5 fold (95%CI: 2.3 to 16.7) in DCI group and 3.4 fold (95%CI: 1.0 to 5.8) in Non-DCI group; miR-324: 4924 fold (95%CI: 2620 to 7228) in DCI group and 4545 fold (95%CI: 2408 to 6683) in non-DCI group]. However, there were no significant differences in fold changes between SAH patients with and without DCI [fold change ratios (mean+/-SD): 2.7+/-4.2 and 1.1+/-1.1 for miRNA-132 and miRNA-324].
  
  
  CONCLUSIONS
  Our study demonstrated that as compared to healthy control, miR-132 and miR-324 showed a upregulation in both SAH DCI and Non-DCI groups. However, the differences between the SAH DCI and non-DCI groups were not statistically significant.
authors.affiliation: Division of Neurosurgery, Department of Surgery, Prince of Wales Hospital, The Chinese University of Hong Kong, HKSAR, China.
authors.forename: Xian Wei
authors.initials: XW
authors.lastname: Su
authors.affiliation: Division of Neurosurgery, Department of Surgery, Prince of Wales Hospital, The Chinese University of Hong Kong, HKSAR, China.
authors.forename: Anna Ho Yin
authors.initials: AH
authors.lastname: Chan
authors.affiliation: Division of Neurosurgery, Department of Surgery, Prince of Wales Hospital, The Chinese University of Hong Kong, HKSAR, China.|School of Biomedical Science, The Chinese University of Hong Kong, HKSAR, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Lu
authors.affiliation: Division of Neurosurgery, Department of Surgery, Prince of Wales Hospital, The Chinese University of Hong Kong, HKSAR, China.
authors.forename: Marie
authors.initials: M
authors.lastname: Lin
authors.affiliation: Division of Neurosurgery, Department of Surgery, Prince of Wales Hospital, The Chinese University of Hong Kong, HKSAR, China.
authors.forename: Johnny
authors.initials: J
authors.lastname: Sze
authors.affiliation: School of Biomedical Science, The Chinese University of Hong Kong, HKSAR, China.
authors.forename: Jing Ye
authors.initials: JY
authors.lastname: Zhou
authors.affiliation: Division of Neurosurgery, Department of Surgery, Prince of Wales Hospital, The Chinese University of Hong Kong, HKSAR, China.
authors.forename: Wai Sang
authors.initials: WS
authors.lastname: Poon
authors.affiliation: School of Biomedical Science, The Chinese University of Hong Kong, HKSAR, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Division of Neurosurgery, Department of Surgery, Prince of Wales Hospital, The Chinese University of Hong Kong, HKSAR, China.
authors.forename: Vera Zhi Yuan
authors.initials: VZ
authors.lastname: Zheng
authors.affiliation: Division of Neurosurgery, Department of Surgery, Prince of Wales Hospital, The Chinese University of Hong Kong, HKSAR, China.
authors.forename: George Kwok Chu
authors.initials: GK
authors.lastname: Wong
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers; D016022:Case-Control Studies; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D002532:Intracranial Aneurysm / Q000150:complications*; D008297:Male; D035683:MicroRNAs / Q000097:blood / Q000235:genetics*; D008875:Middle Aged; D012372:ROC Curve; D013345:Subarachnoid Hemorrhage / Q000097:blood* / Q000209:etiology*; D055815:Young Adult
chemical_list: D015415:Biomarkers; C547077:MIRN132 microRNA, human; C000599310:MIRN324 microRNA, human; D035683:MicroRNAs