title: Detection and Quantification of Free Radicals in Peroxisomal Disorders: A Comparative Study with Oxidative Stress Parameters.
abstract: BACKGROUND
  Free radicals have been thought to participate in pathogenesis of peroxisomal disorders.
  
  
  OBJECTIVE
  The aim of the work is to detect free oxide radicals in blood of patients with peroxisomal disorders and to study their relation with various oxidative stress parameters.
  
  
  METHODS
  Twenty patients with peroxisomal disorders and 14 age and sex matched healthy subjects were included in the study. Patients with peroxisomal disorders were subdivided according to diagnosis into peroxisomal biogenesis disorders and single enzyme deficiency. Oxidative stress was evaluated in both patients and control subjects by assessment of free radicals, malondialdehyde, nitric oxide metabolites and superoxide dismutase.
  
  
  RESULTS
  There was increase in free radicals, malondialdehyde, nitric oxide metabolites in patients compared with control subjects. However, there was decrease in superoxide dismutase levels in patients compared with control subjects.
  
  
  CONCLUSIONS
  We concluded that there is excess free radicals production accompanied with decrease in antioxidant defenses in patients with peroxisomal disorders. These results strongly support a role of free radicals in the pathophysiology of peroxisomal disorders and strengthen the importance of oxidative stress phenomenon in peroxisomal disorders pathogenesis.
authors.affiliation: Professor, Department of Clinical Pathology, National Research Centre , Egypt .
authors.forename: Sohair Abd-El Mawgood
authors.initials: SA
authors.lastname: Abd-Elmaksoud
authors.affiliation: Professor, Department of Clinical Genetics, National Research Centre , Egypt .
authors.forename: Hala
authors.initials: H
authors.lastname: El-Bassyouni
authors.affiliation: Professor, Department of Clinical Genetics, National Research Centre , Egypt .
authors.forename: Hanan
authors.initials: H
authors.lastname: Afifi
authors.affiliation: Researcher, Department of Clinical Genetics, National Research Centre , Egypt .
authors.forename: Manal Micheal
authors.initials: MM
authors.lastname: Thomas
authors.affiliation: Researcher, Department of Clinical Pathology, National Research Centre , Egypt .
authors.forename: Alshaymaa Ahmed
authors.initials: AA
authors.lastname: Ibrahim
authors.affiliation: Researcher, Department of Clinical Pathology, National Research Centre , Egypt .
authors.forename: Aliaa
authors.initials: A
authors.lastname: Shalaby
authors.affiliation: Lecturer, Department of Paediatric, Faculty of Medicine, Cairo University , Egypt .
authors.forename: Tamer Ahmed Abdel
authors.initials: TA
authors.lastname: Hamid
authors.affiliation: Researcher, Department of Paediatric, National Research Centre , Egypt .
authors.forename: Nehal Abdel
authors.initials: NA
authors.lastname: Hamid
authors.affiliation: Lecturer, Department of Clinical and Chemical Pathology, Faculty of Medicine, Cairo University , Egypt .
authors.forename: Hany
authors.initials: H
authors.lastname: El-Ghobary
keywords: Malondialdehyde; Nitric oxide; Superoxide dismutase