title: Signaling Responses After Varying Sequencing of Strength and Endurance Training in a Fed State.
abstract: OBJECTIVE
  To compare anabolic signaling responses to differing sequences of concurrent strength and endurance training in a fed state.
  
  
  METHODS
  Eighteen resistance-trained men were randomly assigned to the following experimental conditions: strength training (ST), strength followed by endurance training (ST-END), or endurance followed by strength training (END-ST). Muscle tissue samples were taken from the vastus lateralis before each exercise protocol, on cessation of exercise, and 1 h after cessation of strength training. Tissue was analyzed for total and phosphorylated (p-) signaling proteins linked to the mTOR and AMPK networks.
  
  
  RESULTS
  Strength-training performance was similar between ST, ST-END, and END-ST. p-S6k1 was elevated from baseline 1 h posttraining in ST and ST-END (both P < .05). p-4E-BP1 was significantly lower than baseline post-ST (P = .01), whereas at 1 h postexercise in the ST-END condition p-4E-BP1 was significantly greater than postexercise (P = .04). p-ACC was elevated from baseline both postexercise and 1 h postexercise (both P < .05) in the END-ST condition. AMPK, mTOR, p38, PKB, and eEF2 responded similarly to ST, ST-END, and END-ST. Signaling responses to ST, ST-END, and END were largely similar. As such it cannot be ascertained which sequence of concurrent strength and endurance training is most favorable in promoting anabolic signaling.
  
  
  CONCLUSIONS
  In the case of the current study an acute bout of concurrent training of differing sequences elicited similar responses of the AMPK and mTOR networks.
authors.forename: Thomas W
authors.initials: TW
authors.lastname: Jones
authors.forename: Ian H
authors.initials: IH
authors.lastname: Walshe
authors.forename: David L
authors.initials: DL
authors.lastname: Hamilton
authors.forename: Glyn
authors.initials: G
authors.lastname: Howatson
authors.forename: Mark
authors.initials: M
authors.lastname: Russell
authors.forename: Oliver J
authors.initials: OJ
authors.lastname: Price
authors.forename: Alan St Clair
authors.initials: AS
authors.lastname: Gibson
authors.forename: Duncan N
authors.initials: DN
authors.lastname: French
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000378:metabolism; D004435:Eating; D006801:Humans; D008297:Male; D053580:Muscle Strength / Q000502:physiology*; D018482:Muscle, Skeletal / Q000378:metabolism*; D010807:Physical Endurance / Q000502:physiology*; D055070:Resistance Training / Q000379:methods*; D015398:Signal Transduction; D058570:TOR Serine-Threonine Kinases / Q000378:metabolism; D055815:Young Adult
chemical_list: C546842:MTOR protein, human; D058570:TOR Serine-Threonine Kinases; D055372:AMP-Activated Protein Kinases
keywords: AMPK; anabolic signaling; combined exercise; exercise order; mTOR