title: Current reprogramming systems in regenerative medicine: from somatic cells to induced pluripotent stem cells.
abstract: Induced pluripotent stem cells (iPSCs) paved the way for research fields including cell therapy, drug screening, disease modeling and the mechanism of embryonic development. Although iPSC technology has been improved by various delivery systems, direct transduction and small molecule regulation, low reprogramming efficiency and genomic modification steps still inhibit its clinical use. Improvements in current vectors and the exploration of novel vectors are required to balance efficiency and genomic modification for reprogramming. Herein, we set out a comprehensive analysis of current reprogramming systems for the generation of iPSCs from somatic cells. By clarifying advantages and disadvantages of the current reprogramming systems, we are striding toward an effective route to generate clinical grade iPSCs.
authors.affiliation: Collaborative Innovation Center for Diagnosis & Treatment of Infectious Diseases, State Key Laboratory for Diagnosis & Treatment of Infectious Diseases, School of Medicine, First Affiliated Hospital, Zhejiang University, Hangzhou, Zhejiang, PR China.
authors.forename: Chenxia
authors.initials: C
authors.lastname: Hu
authors.affiliation: Collaborative Innovation Center for Diagnosis & Treatment of Infectious Diseases, State Key Laboratory for Diagnosis & Treatment of Infectious Diseases, School of Medicine, First Affiliated Hospital, Zhejiang University, Hangzhou, Zhejiang, PR China.
authors.forename: Lanjuan
authors.initials: L
authors.lastname: Li
mesh_terms: D065150:Cellular Reprogramming; D044127:Epigenesis, Genetic; D005822:Genetic Vectors / Q000378:metabolism; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology*; D044968:Regenerative Medicine; D014161:Transduction, Genetic
keywords: efficiency; genomic modification; induced pluripotent stem cells; reprogramming system; vector