title: Polymorphisms in the promoter region of the bovine lactoferrin gene influence milk somatic cell score and milk production traits in Chinese Holstein cows.
abstract: Lactoferrin is an iron-binding protein found in cow's milk that plays an important role in preventing mastitis caused by intramammary infection. In this study, 20 Chinese Holstein cows were selected randomly for PCR amplification and sequencing of the bovine lactoferrin gene promoter region and used for SNP discovery in the region between nucleotide positions -461 to -132. Three SNPs (-270T>C, -190G>A and -156A>G) were identified in bovine lactoferrin, then Chinese Holstein cows (n=866) were genotyped using Sequenom MassARRAY (Sequenom Inc., San Diego, CA) based on the previous SNP information in this study, and the associations between SNPs or haplotype and milk somatic cell score (SCS) and production traits were analyzed by the least squares method in the GLM procedure of SAS. SNPs -270T>C and -156A>G showed close linkage disequilibrium (r(2)=0.76). The SNP -190G>A showed a significant association with SCS, and individuals with genotype GG had higher SCS than genotypes AG and AA. Associations were found between the SNPs -270T>C and -190G>A with SCS and the milk composition. The software MatInspector revealed that these SNPs were located within several potential transcription factor binding sites, including NF-κB p50, KLF7 and SP1, and may alter gene expression, but further investigation will be required to elucidate the biological and practical relevance of these SNPs.
authors.affiliation: College of Animal Science and Technology, Yangzhou University, 225009 Yangzhou, PR China; College of Animal Science and Technology, Huazhong Agricultural University, 430070 Wuhan, PR China. Electronic address: cattle@yzu.edu.cn.
authors.forename: Yongjiang
authors.initials: Y
authors.lastname: Mao
authors.affiliation: College of Animal Science and Technology, Yangzhou University, 225009 Yangzhou, PR China.
authors.forename: Xiaorui
authors.initials: X
authors.lastname: Zhu
authors.affiliation: College of Animal Science and Technology, Yangzhou University, 225009 Yangzhou, PR China.
authors.forename: Shiyu
authors.initials: S
authors.lastname: Xing
authors.affiliation: College of Animal Science and Technology, Yangzhou University, 225009 Yangzhou, PR China.
authors.forename: Meirong
authors.initials: M
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Technology, Yangzhou University, 225009 Yangzhou, PR China.
authors.forename: Huimin
authors.initials: H
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Technology, Yangzhou University, 225009 Yangzhou, PR China.
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Wang
authors.affiliation: Center for Genetic Improvement of Livestock, Department of Animal and Poultry Science, University of Guelph, Guelph, Ontario N1G 2W1, Canada.
authors.forename: Niel
authors.initials: N
authors.lastname: Karrow
authors.affiliation: Center for Genetic Improvement of Livestock, Department of Animal and Poultry Science, University of Guelph, Guelph, Ontario N1G 2W1, Canada.
authors.forename: Liguo
authors.initials: L
authors.lastname: Yang
authors.affiliation: College of Animal Science and Technology, Yangzhou University, 225009 Yangzhou, PR China.
authors.forename: Zhangping
authors.initials: Z
authors.lastname: Yang
mesh_terms: D000818:Animals; D002417:Cattle / Q000235:genetics* / Q000378:metabolism; D002452:Cell Count / Q000662:veterinary; D002681:China; D005260:Female; D007774:Lactation; D007781:Lactoferrin / Q000235:genetics* / Q000378:metabolism; D008892:Milk / Q000737:chemistry* / Q000378:metabolism*; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic
chemical_list: D007781:Lactoferrin
keywords: Bovine lactoferrin gene; Milk production traits; SNP; Somatic cell score