title: Correlation of Particle Traversals with Clonogenic Survival Using Cell-Fluorescent Ion Track Hybrid Detector.
abstract: Development of novel approaches linking the physical characteristics of particles with biological responses are of high relevance for the field of particle therapy. In radiobiology, the clonogenic survival of cells is considered the gold standard assay for the assessment of cellular sensitivity to ionizing radiation. Toward further development of next generation biodosimeters in particle therapy, cell-fluorescent ion track hybrid detector (Cell-FIT-HD) was recently engineered by our group and successfully employed to study physical particle track information in correlation with irradiation-induced DNA damage in cell nuclei. In this work, we investigated the feasibility of Cell-FIT-HD as a tool to study the effects of clinical beams on cellular clonogenic survival. Tumor cells were grown on the fluorescent nuclear track detector as cell culture, mimicking the standard procedures for clonogenic assay. Cell-FIT-HD was used to detect the spatial distribution of particle tracks within colony-initiating cells. The physical data were associated with radiation-induced foci as surrogates for DNA double-strand breaks, the hallmark of radiation-induced cell lethality. Long-term cell fate was monitored to determine the ability of cells to form colonies. We report the first successful detection of particle traversal within colony-initiating cells at subcellular resolution using Cell-FIT-HD.
authors.affiliation: German Cancer Consortium, Translational Radiation Oncology, National Center for Tumor Diseases, German Cancer Research Center, Heidelberg University Medical School , Heidelberg , Germany ; Heidelberg Ion Therapy Center , Heidelberg , Germany ; Heidelberg Institute of Radiation Oncology, National Center for Radiation Research in Oncology , Heidelberg , Germany.
authors.forename: Ivana
authors.initials: I
authors.lastname: Dokic
authors.affiliation: German Cancer Consortium, Translational Radiation Oncology, National Center for Tumor Diseases, German Cancer Research Center, Heidelberg University Medical School , Heidelberg , Germany ; Heidelberg Ion Therapy Center , Heidelberg , Germany ; Heidelberg Institute of Radiation Oncology, National Center for Radiation Research in Oncology , Heidelberg , Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Niklas
authors.affiliation: German Cancer Consortium, Translational Radiation Oncology, National Center for Tumor Diseases, German Cancer Research Center, Heidelberg University Medical School , Heidelberg , Germany ; Heidelberg Ion Therapy Center , Heidelberg , Germany ; Heidelberg Institute of Radiation Oncology, National Center for Radiation Research in Oncology , Heidelberg , Germany.
authors.forename: Ferdinand
authors.initials: F
authors.lastname: Zimmermann
authors.affiliation: Heidelberg Ion Therapy Center , Heidelberg , Germany ; National Center for Oncological Hadrontherapy , Pavia , Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Mairani
authors.affiliation: German Cancer Consortium, Translational Radiation Oncology, National Center for Tumor Diseases, German Cancer Research Center, Heidelberg University Medical School , Heidelberg , Germany ; Heidelberg Ion Therapy Center , Heidelberg , Germany ; Heidelberg Institute of Radiation Oncology, National Center for Radiation Research in Oncology , Heidelberg , Germany.
authors.forename: Philipp
authors.initials: P
authors.lastname: Seidel
authors.affiliation: Light Microscopy Facility, German Cancer Research Center , Heidelberg , Germany.
authors.forename: Damir
authors.initials: D
authors.lastname: Krunic
authors.affiliation: Heidelberg Ion Therapy Center , Heidelberg , Germany ; Heidelberg Institute of Radiation Oncology, National Center for Radiation Research in Oncology , Heidelberg , Germany ; Division of Medical Physics in Radiation Oncology, German Cancer Research Center , Heidelberg , Germany.
authors.forename: Oliver
authors.initials: O
authors.lastname: Jäkel
authors.affiliation: German Cancer Consortium, Translational Radiation Oncology, National Center for Tumor Diseases, German Cancer Research Center, Heidelberg University Medical School , Heidelberg , Germany ; Heidelberg Ion Therapy Center , Heidelberg , Germany ; Heidelberg Institute of Radiation Oncology, National Center for Radiation Research in Oncology , Heidelberg , Germany.
authors.forename: Jürgen
authors.initials: J
authors.lastname: Debus
authors.affiliation: Heidelberg Institute of Radiation Oncology, National Center for Radiation Research in Oncology , Heidelberg , Germany ; Division of Medical Physics in Radiation Oncology, German Cancer Research Center , Heidelberg , Germany.
authors.forename: Steffen
authors.initials: S
authors.lastname: Greilich
authors.affiliation: German Cancer Consortium, Translational Radiation Oncology, National Center for Tumor Diseases, German Cancer Research Center, Heidelberg University Medical School , Heidelberg , Germany ; Heidelberg Ion Therapy Center , Heidelberg , Germany ; Heidelberg Institute of Radiation Oncology, National Center for Radiation Research in Oncology , Heidelberg , Germany.
authors.forename: Amir
authors.initials: A
authors.lastname: Abdollahi
keywords: 53BP1; DNA damage foci; carbon ion irradiation; clonogenic survival; fluorescent nuclear track detector