title: Fatty Acid Profile and Biological Activities of Linseed and Rapeseed Oils.
abstract: It has been postulated that fatty acids found in edible oils may exert beneficial health effects by the modulation of signaling pathways regulating cell differentiation and proliferation, especially in the treatment of cardiovascular diseases. In the present study, the biological effects of selected edible oils--linseed (LO) and rapeseed (RO) oils--were tested in vitro on fibroblast cells. The fatty acid profile of the oils was determined using gas chromatography and FTIR spectroscopy. LO was found to be rich in α-linolenic acid (ALA), whereas oleic acid was the most abundant species in RO. Fatty acids were taken up by the cells and promoted cell proliferation. No oxidative stress-mediated cytotoxic or genotoxic effects were observed after oil stimulation. Oils ameliorated the process of wound healing as judged by improved migration of fibroblasts to the wounding area. As ALA-rich LO exhibited the most potent wound healing activity, ALA may be considered a candidate for promoting the observed effect.
authors.affiliation: Department of Biochemistry and Cell Biology, University of Rzeszow, Zelwerowicza 4, 35-601 Rzeszow, Poland. alewinska@o2.pl.
authors.forename: Anna
authors.initials: A
authors.lastname: Lewinska
authors.affiliation: Department of Plant Physiology, University of Rzeszow, Werynia 502, 36-100 Kolbuszowa, Poland. jaze28@interia.pl.
authors.forename: Jacek
authors.initials: J
authors.lastname: Zebrowski
authors.affiliation: Department of Botany, University of Rzeszow, Werynia 502, 36-100 Kolbuszowa, Poland. duda.ma@wp.pl.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Duda
authors.affiliation: Department of Animal Physiology and Reproduction, University of Rzeszow, Werynia 502, 36-100 Kolbuszowa, Poland. agorka@ur.edu.pl.
authors.forename: Anna
authors.initials: A
authors.lastname: Gorka
authors.affiliation: Department of Genetics, University of Rzeszow, Rejtana 16C, 35-959 Rzeszow, Poland. mawnuk@gmail.com.
authors.forename: Maciej
authors.initials: M
authors.lastname: Wnuk
mesh_terms: D000818:Animals; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D005227:Fatty Acids / Q000378:metabolism*; D005229:Fatty Acids, Monounsaturated; D005347:Fibroblasts / Q000187:drug effects; D008043:Linseed Oil / Q000378:metabolism* / Q000494:pharmacology*; D051379:Mice; D041681:NIH 3T3 Cells; D019301:Oleic Acid / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D010938:Plant Oils / Q000378:metabolism* / Q000494:pharmacology*; D000074262:Rapeseed Oil; D014945:Wound Healing / Q000187:drug effects; D017962:alpha-Linolenic Acid / Q000378:metabolism
chemical_list: D005227:Fatty Acids; D005229:Fatty Acids, Monounsaturated; D010938:Plant Oils; D000074262:Rapeseed Oil; D017962:alpha-Linolenic Acid; D019301:Oleic Acid; D008043:Linseed Oil
keywords: FTIR spectroscopy; fibroblasts; gas chromatography; linseed oil; rapeseed oil; scratch wound healing assay