title: A simple molecular beacon with duplex-specific nuclease amplification for detection of microRNA.
abstract: MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene activity, promoting or inhibiting cell proliferation, migration and apoptosis. Abnormal expression of miRNAs is associated with many diseases. Therefore, it is essential to establish a simple, rapid and sensitive miRNA detection method. In this paper, based on a simple molecular beacon (MB) and duplex-specific nuclease (DSN), we developed a target recycling amplification method for miRNA detection. By controlling the number of stem bases to 5, the MB probe used in this method can be prevented from hydrolysis by DSN without special modification. This assay is direct and simple to quantitatively detect miRNA with high sensitivity and specificity. The MB probe design provides a new strategy for nuclease-based amplification reaction.
authors.affiliation: Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, College of Chemistry and Environmental Science, Hebei University, Baoding 071002, Hebei, P. R. China.
authors.forename: Yingcun
authors.initials: Y
authors.lastname: Li
authors.forename: Jiangyan
authors.initials: J
authors.lastname: Zhang
authors.forename: Jingjing
authors.initials: J
authors.lastname: Zhao
authors.forename: Likun
authors.initials: L
authors.lastname: Zhao
authors.forename: Yongqiang
authors.initials: Y
authors.lastname: Cheng
authors.forename: Zhengping
authors.initials: Z
authors.lastname: Li
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D003851:Deoxyribonucleases / Q000378:metabolism*; D005189:False Positive Reactions; D006367:HeLa Cells; D006801:Humans; D057230:Limit of Detection; D016014:Linear Models; D035683:MicroRNAs / Q000032:analysis* / Q000737:chemistry / Q000235:genetics*; D008958:Models, Molecular; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D009690:Nucleic Acid Conformation; D015345:Oligonucleotide Probes / Q000737:chemistry*
chemical_list: D035683:MicroRNAs; D015345:Oligonucleotide Probes; D003851:Deoxyribonucleases