title: Kapok Fiber: A Natural Biomaterial for Highly Specific and Efficient Enrichment of Sialoglycopeptides.
abstract: Cancer development and chronic diseases are associated with the overexpression of sialoglycans terminated to the surface proteins and lipids of cancer cells compared with normal cells. The isolation and detection of sialoglycopeptides from complex peptides mixture still remain challenges due to their low abundance, low ionization, and losses of sialic acid residues and water molecule during analytical processes. In this study, kapok fiber, a natural fiber derived from the kapok tree (Bombax ceiba L.), has shown excellent capability to specifically and efficiently enrich sialoglycopeptides, without losses of sialic acid residues and water molecule from sialoglycans. The main components on the surface of kapok fiber are syringyl and guaiacyl lignins which play an important role in isolating sialoglycopeptides from complex peptide mixtures.
authors.affiliation: Department of Biophysics and Structural Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & School of Basic Medicine, Peking Union Medical College , Beijing 100005, PR China.
authors.forename: Yujie
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Shandong Non-Metallic Material Institute , Jinan 250031, PR China.
authors.forename: Yuanjun
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Biophysics and Structural Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & School of Basic Medicine, Peking Union Medical College , Beijing 100005, PR China.
authors.forename: Dan
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Department of Biophysics and Structural Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & School of Basic Medicine, Peking Union Medical College , Beijing 100005, PR China.
authors.forename: Ruiqing
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Department of Biophysics and Structural Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & School of Basic Medicine, Peking Union Medical College , Beijing 100005, PR China.
authors.forename: Zhili
authors.initials: Z
authors.lastname: Li
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D035163:Bombax / Q000737:chemistry*; D006801:Humans; D015394:Molecular Structure; D010316:Particle Size; D012795:Sialoglycoproteins / Q000737:chemistry / Q000302:isolation & purification*; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D012795:Sialoglycoproteins