title: A Lysosome-Targeting AIEgen for Autophagy Visualization.
abstract: In this work, a morpholine-functionalized aggregation-induced emission luminogen (AIEgen), AIE-LysoY, is reported for lysosomal imaging and autophagy visualization. To attain outstanding imaging contrast, AIE-LysoY is equipped with excited state intramolecular proton transfer (ESIPT) characteristic. AIE-LysoY provides a new platform for lysosome visualization with good biocompatibility, large Stokes shift, superior signal-to-noise ratio, and high photostability.
authors.affiliation: HKUST Shenzhen Research Institute, Nanshan, Shenzhen, 518057, China.|Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, Institute for Advanced Study, Division of Biomedical Engineering, Division of Life Science, State Key Laboratory of Molecular Neuroscience and Institute of Molecular Functional Materials, The Hong Kong University of Science & Technology (HKUST), Clear Water Bay, Kowloon, Hong Kong, China.
authors.forename: Chris Wai Tung
authors.initials: CW
authors.lastname: Leung
authors.affiliation: HKUST Shenzhen Research Institute, Nanshan, Shenzhen, 518057, China.|Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, Institute for Advanced Study, Division of Biomedical Engineering, Division of Life Science, State Key Laboratory of Molecular Neuroscience and Institute of Molecular Functional Materials, The Hong Kong University of Science & Technology (HKUST), Clear Water Bay, Kowloon, Hong Kong, China.
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Wang
authors.affiliation: HKUST Shenzhen Research Institute, Nanshan, Shenzhen, 518057, China.|Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, Institute for Advanced Study, Division of Biomedical Engineering, Division of Life Science, State Key Laboratory of Molecular Neuroscience and Institute of Molecular Functional Materials, The Hong Kong University of Science & Technology (HKUST), Clear Water Bay, Kowloon, Hong Kong, China.
authors.forename: Engui
authors.initials: E
authors.lastname: Zhao
authors.affiliation: School of Chemistry, The University of Melbourne, Parkville, VIC, 3010, Australia.
authors.forename: Yuning
authors.initials: Y
authors.lastname: Hong
authors.affiliation: HKUST Shenzhen Research Institute, Nanshan, Shenzhen, 518057, China.|Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, Institute for Advanced Study, Division of Biomedical Engineering, Division of Life Science, State Key Laboratory of Molecular Neuroscience and Institute of Molecular Functional Materials, The Hong Kong University of Science & Technology (HKUST), Clear Water Bay, Kowloon, Hong Kong, China.
authors.forename: Sijie
authors.initials: S
authors.lastname: Chen
authors.affiliation: HKUST Shenzhen Research Institute, Nanshan, Shenzhen, 518057, China.|Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, Institute for Advanced Study, Division of Biomedical Engineering, Division of Life Science, State Key Laboratory of Molecular Neuroscience and Institute of Molecular Functional Materials, The Hong Kong University of Science & Technology (HKUST), Clear Water Bay, Kowloon, Hong Kong, China.
authors.forename: Ryan Tsz Kin
authors.initials: RT
authors.lastname: Kwok
authors.affiliation: HKUST Shenzhen Research Institute, Nanshan, Shenzhen, 518057, China.|Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, Institute for Advanced Study, Division of Biomedical Engineering, Division of Life Science, State Key Laboratory of Molecular Neuroscience and Institute of Molecular Functional Materials, The Hong Kong University of Science & Technology (HKUST), Clear Water Bay, Kowloon, Hong Kong, China.
authors.forename: Anakin Chun Sing
authors.initials: AC
authors.lastname: Leung
authors.affiliation: Department of Chemistry and Chemical Engineering, Shenzhen University, Shenzhen, 518060, China.
authors.forename: Rongsen
authors.initials: R
authors.lastname: Wen
authors.affiliation: Department of Chemistry and Chemical Engineering, Shenzhen University, Shenzhen, 518060, China.
authors.forename: Bingshi
authors.initials: B
authors.lastname: Li
authors.affiliation: HKUST Shenzhen Research Institute, Nanshan, Shenzhen, 518057, China.|Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, Institute for Advanced Study, Division of Biomedical Engineering, Division of Life Science, State Key Laboratory of Molecular Neuroscience and Institute of Molecular Functional Materials, The Hong Kong University of Science & Technology (HKUST), Clear Water Bay, Kowloon, Hong Kong, China.
authors.forename: Jacky Wing Yip
authors.initials: JW
authors.lastname: Lam
authors.affiliation: HKUST Shenzhen Research Institute, Nanshan, Shenzhen, 518057, China.|Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, Institute for Advanced Study, Division of Biomedical Engineering, Division of Life Science, State Key Laboratory of Molecular Neuroscience and Institute of Molecular Functional Materials, The Hong Kong University of Science & Technology (HKUST), Clear Water Bay, Kowloon, Hong Kong, China.|Guangdong Innovative Research Team, SCUT-HKUST Joint Research Laboratory, State Key Laboratory of Luminescent Materials and Devices, South China University of Technology (SCUT), Guangzhou, 510640, China.
authors.forename: Ben Zhong
authors.initials: BZ
authors.lastname: Tang
mesh_terms: D001343:Autophagy; D001672:Biocompatible Materials / Q000737:chemistry; D002470:Cell Survival; D006367:HeLa Cells; D006801:Humans; D008247:Lysosomes / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D015335:Molecular Probes / Q000737:chemistry*; D009025:Morpholines / Q000737:chemistry*; D011522:Protons; D059629:Signal-To-Noise Ratio
chemical_list: D001672:Biocompatible Materials; D015335:Molecular Probes; D009025:Morpholines; D011522:Protons; C037574:morpholine
keywords: aggregation-induced emission; autophagy; lysosome imaging; rapamycin