title: Successful management of severe unilateral chemical burns in children using simple limbal epithelial transplantation (SLET).
abstract: To study the outcomes of simple limbal epithelial transplantation (SLET) for unilateral total limbal stem cell deficiency (LSCD) secondary to severe ocular surface burns in children.
  
  
  
  Retrospective interventional case series was performed at a private referral tertiary care centre. Children less than 15 years of age who underwent autologous SLET for total LSCD and had a minimum follow-up of 6 months were included in the study. Demographic and clinical data were recorded in a predesigned form. All patients underwent SLET with a standardised technique. The outcome was defined as complete success (completely epithelialised, avascular corneal surface), partial success (focal recurrence of symblepharon not involving the visual axis) and failure (unstable ocular surface with persistent epithelial defects/symblepharon recurrence involving the visual axis).
  
  
  
  The mean age was 5.75 years (range 2-12). The male to female ratio was 3:1. All eyes (four) presented in the acute phase, had grade 6 chemical injury (Dua classification) and underwent amniotic membrane transplantation at presentation. The mean interval between initial injury and SLET was 6 months (range 4.5-8). The outcome was complete success and partial success in one-fourth and three-fourths of cases, respectively. The overall follow-up was 12-60 months. Pre-SLET visual acuities were hand motions (one eye) and perception of light (three eyes). Post-SLET visual acuities were counting fingers close to face (one eye), 6/36 (two eyes) and 6/18 (one eye) at final follow-up. Cases with partial success underwent repeat SLET with conjunctival autograft, after which the outcome was complete success in all cases at varied follow-up intervals (13-36 months).
  
  
  
  SLET appears to be a promising technique for treatment of LSCD secondary to ocular surface burns in children.
authors.affiliation: Cornea and Anterior Segment Services, Sanjivni Eye Care, Model Town, Ambala City, Haryana, India.
authors.forename: Vikas
authors.initials: V
authors.lastname: Mittal
authors.affiliation: Cornea, DrishtiCone Eye Care, New Delhi, India.
authors.forename: Rajat
authors.initials: R
authors.lastname: Jain
authors.affiliation: Vitreo-retina Services, Sanjivni Eye Care, Ambala City, Haryana, India.
authors.forename: Ruchi
authors.initials: R
authors.lastname: Mittal
authors.affiliation: Pediatric Ophthalmology Services, Sanjivni Eye Care, Ambala City, Haryana, India.
authors.forename: Urvish
authors.initials: U
authors.lastname: Vashist
authors.affiliation: Cornea and Anterior Segment Services, Model Town, Sanjivni Eye Care, Ambala City, Haryana, India.
authors.forename: Purvasha
authors.initials: P
authors.lastname: Narang
mesh_terms: D002057:Burns, Chemical / Q000175:diagnosis / Q000601:surgery*; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D016039:Corneal Transplantation / Q000379:methods*; D005126:Eye Burns / Q000175:diagnosis / Q000601:surgery*; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D016850:Limbus Corneae / Q000166:cytology / Q000601:surgery*; D008297:Male; D012189:Retrospective Studies; D033581:Stem Cell Transplantation / Q000379:methods*; D013234:Stem Cells / Q000166:cytology*; D014182:Transplantation, Autologous; D015599:Trauma Severity Indices; D016896:Treatment Outcome; D014792:Visual Acuity
keywords: Child health (paediatrics); Cornea; Ocular surface; Stem Cells; Trauma