title: Human mesenchymal stem cell behavior on segmented polyurethanes prepared with biologically active chain extenders.
abstract: The development of elastomeric, bioresorbable and biocompatible segmented polyurethanes (SPUs) for use in tissue-engineering applications has attracted considerable interest because of the existing need of mechanically tunable scaffolds for regeneration of different tissues, but the incorporation of osteoinductive molecules into SPUs has been limited. In this study, SPUs were synthesized from poly (ε-caprolactone)diol, 4,4'-methylene bis(cyclohexyl isocyanate) using biologically active compounds such as ascorbic acid, L-glutamine, β-glycerol phosphate, and dexamethasone as chain extenders. Fourier transform infrared spectroscopy (FTIR) revealed the formation of both urethanes and urea linkages while differential scanning calorimetry, dynamic mechanical analysis, X-ray diffraction and mechanical testing showed that these polyurethanes were semi-crystalline polymers exhibiting high deformations. Cytocompatibility studies showed that only SPUs containing β-glycerol phosphate supported human mesenchymal stem cell adhesion, growth, and osteogenic differentiation, rendering them potentially suitable for bone tissue regeneration, whereas other SPUs failed to support either cell growth or osteogenic differentiation, or both. This study demonstrates that modification of SPUs with osteogenic compounds can lead to new cytocompatible polymers for regenerative medicine applications.
authors.affiliation: Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, 313 Ferst Drive, Atlanta, GA, 30332, USA.
authors.forename: Taylor E
authors.initials: TE
authors.lastname: Kavanaugh
authors.affiliation: Woodruff School of Mechanical Engineering and Petit Institute for Bioengineering and Biosciences, Georgia Institute of Technology, 315 Ferst Drive, Atlanta, GA, 30332, USA.
authors.forename: Amy Y
authors.initials: AY
authors.lastname: Clark
authors.affiliation: CONACYT - Departamento de Física, Universidad de Sonora, Luis Encinas y Rosales, 83000, Hermosillo, Sonora, Mexico.
authors.forename: Lerma H
authors.initials: LH
authors.lastname: Chan-Chan
authors.affiliation: CONACYT - Departamento de Física, Universidad de Sonora, Luis Encinas y Rosales, 83000, Hermosillo, Sonora, Mexico.
authors.forename: Maricela
authors.initials: M
authors.lastname: Ramírez-Saldaña
authors.affiliation: Centro de Investigación Científica de Yucatán A.C., Calle 43 130, Col. Chuburná de Hidalgo, C.P. 97200, Mérida, Yucatán, Mexico.
authors.forename: Rossana F
authors.initials: RF
authors.lastname: Vargas-Coronado
authors.affiliation: Centro de Investigación Científica de Yucatán A.C., Calle 43 130, Col. Chuburná de Hidalgo, C.P. 97200, Mérida, Yucatán, Mexico.
authors.forename: José M
authors.initials: JM
authors.lastname: Cervantes-Uc
authors.affiliation: Centro de Investigación Científica de Yucatán A.C., Calle 43 130, Col. Chuburná de Hidalgo, C.P. 97200, Mérida, Yucatán, Mexico.
authors.forename: Fernando
authors.initials: F
authors.lastname: Hernández-Sánchez
authors.affiliation: Woodruff School of Mechanical Engineering and Petit Institute for Bioengineering and Biosciences, Georgia Institute of Technology, 315 Ferst Drive, Atlanta, GA, 30332, USA.
authors.forename: Andrés J
authors.initials: AJ
authors.lastname: García
authors.affiliation: Centro de Investigación Científica de Yucatán A.C., Calle 43 130, Col. Chuburná de Hidalgo, C.P. 97200, Mérida, Yucatán, Mexico. jvcr@cicy.mx.
authors.forename: Juan V
authors.initials: JV
authors.lastname: Cauich-Rodríguez
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D001842:Bone and Bones / Q000166:cytology / Q000187:drug effects; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D006801:Humans; D008422:Materials Testing; D055595:Mechanical Phenomena; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects* / Q000502:physiology; D010012:Osteogenesis / Q000187:drug effects; D011140:Polyurethanes / Q000737:chemistry*; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D011140:Polyurethanes