title: Immunomodulatory and cellular antioxidant activities of pure compounds from Teucrium ramosissimum Desf.
abstract: Evaluation of the immunomodulatory activity of plant compounds is an interesting and growing area of research. Teucrium ramosissimum Desf. is a native and endemic medicinal plant from the South of Tunisia traditionally used for the treatment of many diseases. The anti-inflammatory activity of apigenin-7-glucoside, genkwanin, and naringenin isolated from T. ramosissimum were assayed. The phagocytic activities of macrophage and lymphocyte proliferation were investigated in the absence and presence of mitogens (lipopolysaccharide [LPS] or lectin). Depending on the concentrations, the compounds affect macrophage functions by modulating their lysosomal enzyme activity and nitric oxide (NO) release. The tested compounds enhance significantly splenocyte proliferation, either with or without mitogen stimulation. In studies to assess any potential effects of apigenin-7-glucoside, genkwanin, and naringenin on innate immunity, the results showed that these compounds significantly enhanced the killing activity of natural killer (NK) cells and cytotoxic activity of the T lymphocyte (CTL) isolated from splenocytes. These results suggest that T. ramosissimum compounds such as apigenin-7-glucoside, genkwanin, and naringenin may be potentially useful for modulating immune cell functions in physiological and pathological conditions.
authors.affiliation: Unit of Bioactive Natural Substances and Biotechnology UR12ES12, Faculty of Pharmacy, University of Monastir, Avicenne Street, Monastir, 5000, Tunisia.|Laboratory of Cellular and Molecular Biology, Faculty of Dental Medicine, University of Monastir, Avicenne Street, Monastir, 5000, Tunisia.
authors.forename: Nouha
authors.initials: N
authors.lastname: Nasr-Bouzaiene
authors.affiliation: Unit of Bioactive Natural Substances and Biotechnology UR12ES12, Faculty of Pharmacy, University of Monastir, Avicenne Street, Monastir, 5000, Tunisia.|Laboratory of Cellular and Molecular Biology, Faculty of Dental Medicine, University of Monastir, Avicenne Street, Monastir, 5000, Tunisia.
authors.forename: Aicha
authors.initials: A
authors.lastname: Sassi
authors.affiliation: Unit of Bioactive Natural Substances and Biotechnology UR12ES12, Faculty of Pharmacy, University of Monastir, Avicenne Street, Monastir, 5000, Tunisia.|Laboratory of Cellular and Molecular Biology, Faculty of Dental Medicine, University of Monastir, Avicenne Street, Monastir, 5000, Tunisia.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Bedoui
authors.affiliation: Unit of Bioactive Natural Substances and Biotechnology UR12ES12, Faculty of Pharmacy, University of Monastir, Avicenne Street, Monastir, 5000, Tunisia.|Laboratory of Cellular and Molecular Biology, Faculty of Dental Medicine, University of Monastir, Avicenne Street, Monastir, 5000, Tunisia.
authors.forename: Mounira
authors.initials: M
authors.lastname: Krifa
authors.affiliation: Unit of Bioactive Natural Substances and Biotechnology UR12ES12, Faculty of Pharmacy, University of Monastir, Avicenne Street, Monastir, 5000, Tunisia. leila.chekir@laposte.net.|Laboratory of Cellular and Molecular Biology, Faculty of Dental Medicine, University of Monastir, Avicenne Street, Monastir, 5000, Tunisia. leila.chekir@laposte.net.
authors.forename: Leila
authors.initials: L
authors.lastname: Chekir-Ghedira
authors.affiliation: Laboratory of Cellular and Molecular Biology, Faculty of Dental Medicine, University of Monastir, Avicenne Street, Monastir, 5000, Tunisia.
authors.forename: Kamel
authors.initials: K
authors.lastname: Ghedira
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D047310:Apigenin / Q000302:isolation & purification / Q000494:pharmacology; D002478:Cells, Cultured / Q000187:drug effects; D004731:Endotoxins / Q000494:pharmacology; D044950:Flavanones / Q000302:isolation & purification / Q000494:pharmacology; D047309:Flavones / Q000302:isolation & purification / Q000494:pharmacology; D007113:Immunity, Innate / Q000187:drug effects*; D007155:Immunologic Factors / Q000302:isolation & purification / Q000494:pharmacology*; D007694:Killer Cells, Natural / Q000187:drug effects; D008213:Lymphocyte Activation / Q000187:drug effects; D008247:Lysosomes / Q000187:drug effects / Q000201:enzymology; D008264:Macrophages / Q000187:drug effects; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009569:Nitric Oxide / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry; D011043:Pokeweed Mitogens / Q000494:pharmacology; D054788:Ribosome Inactivating Proteins / Q000494:pharmacology; D013047:Specific Pathogen-Free Organisms; D013602:T-Lymphocytes, Cytotoxic / Q000187:drug effects* / Q000276:immunology; D031367:Teucrium / Q000737:chemistry*; D014416:Tunisia
chemical_list: D000975:Antioxidants; D004731:Endotoxins; D044950:Flavanones; D047309:Flavones; D007155:Immunologic Factors; D010936:Plant Extracts; D011043:Pokeweed Mitogens; C104076:pokeweed lectin D; D009569:Nitric Oxide; C014568:genkwanin; C044101:endotoxin, Escherichia coli; C057792:apigetrin; D047310:Apigenin; D054788:Ribosome Inactivating Proteins; C005273:naringenin
keywords: Cellular antioxidant activity; Immunomodulation; Teucrium ramosissimum Desf.