title: Tudor-SN Regulates Milk Synthesis and Proliferation of Bovine Mammary Epithelial Cells.
abstract: Tudor staphylococcal nuclease (Tudor-SN) is a highly conserved and ubiquitously expressed multifunctional protein, related to multiple and diverse cell type- and species-specific cellular processes. Studies have shown that Tudor-SN is mainly expressed in secretory cells, however knowledge of its role is limited. In our previous work, we found that the protein level of Tudor-SN was upregulated in the nucleus of bovine mammary epithelial cells (BMEC). In this study, we assessed the role of Tudor-SN in milk synthesis and cell proliferation of BMEC. We exploited gene overexpression and silencing methods, and found that Tudor-SN positively regulates milk synthesis and proliferation via Stat5a activation. Both amino acids (methionine) and estrogen triggered NFκB1 to bind to the gene promoters of Tudor-SN and Stat5a, and this enhanced the protein level and nuclear localization of Tudor-SN and p-Stat5a. Taken together, these results suggest the key role of Tudor-SN in the transcriptional regulation of milk synthesis and proliferation of BMEC under the stimulation of amino acids and hormones.
authors.affiliation: The Key Laboratory of Dairy Science of Education Ministry, Northeast Agricultural University, Harbin 150030, China. aojinxia@163.com.
authors.forename: Jinxia
authors.initials: J
authors.lastname: Ao
authors.affiliation: The Key Laboratory of Dairy Science of Education Ministry, Northeast Agricultural University, Harbin 150030, China. chengjiewei@163.com.
authors.forename: Chengjie
authors.initials: C
authors.lastname: Wei
authors.affiliation: The Key Laboratory of Dairy Science of Education Ministry, Northeast Agricultural University, Harbin 150030, China. siyushenghua@sina.com.
authors.forename: Yu
authors.initials: Y
authors.lastname: Si
authors.affiliation: The Key Laboratory of Dairy Science of Education Ministry, Northeast Agricultural University, Harbin 150030, China. luochaochao839505@163.com.
authors.forename: Chaochao
authors.initials: C
authors.lastname: Luo
authors.affiliation: The Key Laboratory of Dairy Science of Education Ministry, Northeast Agricultural University, Harbin 150030, China. lvwei199063@163.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Lv
authors.affiliation: The Key Laboratory of Dairy Science of Education Ministry, Northeast Agricultural University, Harbin 150030, China. linlu516@163.com.
authors.forename: Ye
authors.initials: Y
authors.lastname: Lin
authors.affiliation: The Key Laboratory of Dairy Science of Education Ministry, Northeast Agricultural University, Harbin 150030, China. c_yingjun@sina.com.
authors.forename: Yingjun
authors.initials: Y
authors.lastname: Cui
authors.affiliation: The Key Laboratory of Dairy Science of Education Ministry, Northeast Agricultural University, Harbin 150030, China. gaoxj5390@sina.com.
authors.forename: Xuejun
authors.initials: X
authors.lastname: Gao
mesh_terms: D000818:Animals; D002417:Cattle; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D004847:Epithelial Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D004967:Estrogens / Q000494:pharmacology; D005260:Female; D020868:Gene Silencing / Q000187:drug effects; D008321:Mammary Glands, Animal / Q000166:cytology*; D008715:Methionine / Q000494:pharmacology; D008892:Milk / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism*; D010766:Phosphorylation / Q000187:drug effects; D011485:Protein Binding / Q000187:drug effects; D050799:STAT5 Transcription Factor / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D004967:Estrogens; D016328:NF-kappa B; D009687:Nuclear Proteins; D050799:STAT5 Transcription Factor; D008715:Methionine
keywords: NFκB1; Stat5; Tudor-SN; cell proliferation; milk synthesis