title: Tryptophan Fluorescence Changes Related to Ca(2+)-ATPase Function.
abstract: Fluorescence measurements as monitored with either extrinsic or intrinsic probes constitute important ways with which to study the molecular properties of macromolecules. With high-quality spectrofluorimeters, it is, e.g., possible kinetically to follow local conformational changes, induced by ligands and inhibitors, with a sensitivity that is unsurpassed by any other physicochemical technique. We demonstrate here with Ca(2+) and two specific inhibitors of SERCA how this can be done by measurements of the intrinsic fluorescence of the tryptophan residues of SERCA.
authors.affiliation: Department of Biomedicine, Aarhus University, Aarhus C, Denmark.|Centre for Membrane Pumps in Cells and Disease, Danish National Research Foundation, Aarhus C, Denmark.
authors.forename: Pankaj
authors.initials: P
authors.lastname: Sehgal
authors.affiliation: Department of Molecular Biology and Genetics, Aarhus University, Aarhus C, Denmark.|Centre for Membrane Pumps in Cells and Disease, Danish National Research Foundation, Aarhus C, Denmark.
authors.forename: Claus
authors.initials: C
authors.lastname: Olesen
authors.affiliation: Department of Biomedicine, Aarhus University, Aarhus C, Denmark. jvm@biomed.au.dk.|Centre for Membrane Pumps in Cells and Disease, Danish National Research Foundation, Aarhus C, Denmark. jvm@biomed.au.dk.
authors.forename: Jesper V
authors.initials: JV
authors.lastname: Møller
mesh_terms: D002118:Calcium / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology; D057927:Hydrophobic and Hydrophilic Interactions; D017434:Protein Structure, Tertiary; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000378:metabolism*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D014364:Tryptophan / Q000737:chemistry*
chemical_list: D004791:Enzyme Inhibitors; D014364:Tryptophan; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases; D002118:Calcium
keywords: Calcium ATPase; Conformational change; Intrinsic fluorescence; Spectrofluorimetry; Tryptophan fluorescence