title: Quantification of structurally related aliphatic amino alcohols in l-valinol by hydrophilic interaction liquid chromatography separation combined with postcolumn derivatization and fluorescence detection.
abstract: The amino alcohols in l-valinol were effectively separated and quantified using hydrophilic interaction chromatography with fluorescence detection. The influence of the mobile phase (salt type, buffer concentration, and pH) on retention was studied. A column TSKgel amide and mobile phase consisting of 10 mM acetate buffer pH 4.0 and acetonitrile (20:80, v/v) provided well-separated symmetric peaks of analytes. Fluorescence detection was performed using postcolumn derivatization with o-phtaldialdehyde/2-mercaptoethanol at an excitation and emission wavelength of 345 and 450 nm, respectively. Simple sample pretreatment and very high sensitivity represent the main advantages of the developed method. After validation, the method was successfully applied to the analysis of commercial samples of l-valinol.
authors.affiliation: Zentiva, k.s. Praha, a Sanofi Company, Praha 10, Czech Republic.
authors.forename: Michal
authors.initials: M
authors.lastname: Douša
authors.affiliation: Zentiva, k.s. Praha, a Sanofi Company, Praha 10, Czech Republic.
authors.forename: Jan
authors.initials: J
authors.lastname: Stach
authors.affiliation: Zentiva, k.s. Praha, a Sanofi Company, Praha 10, Czech Republic.
authors.forename: Petr
authors.initials: P
authors.lastname: Gibala
authors.affiliation: Department of Analytical Chemistry, Regional Centre of Advanced Technologies and Materials, Faculty of Science, Palacký University, Olomouc, Czech Republic.
authors.forename: Karel
authors.initials: K
authors.lastname: Lemr
keywords: Amino alcohols; Elvitegravir; Fluorescence detection; Hydrophilic interaction liquid chromatography; Valinol