title: Life history trait analysis of the entomopathogenic nematode Steinernema feltiae provides the basis for prediction of dauer juvenile yields in monoxenic liquid culture.
abstract: Entomopathogenic nematodes (Steinernema spp.) are used in integrated pest management to control insect pests in cryptic environments. The nematodes are mass produced in monoxenic liquid culture with their symbiotic bacteria Xenorhabdus spp. For a better understanding of nematode population dynamics, the life history traits (LHTs) of the entomopathogenic nematode Steinernema feltiae were assessed at 25 °C by observing single pairs of male and female nematodes using a hanging drop technique. To investigate the influence of different food supplies on nematode reproduction, the LHTs were assessed with a daily supply of 5 ×, 10 × and 20 × 10(9) cells ml(-1) of the nematode's bacterial symbiont Xenorhabdus bovienii in semi-solid nematode growth gelrite (NGG) medium. Increasing bacterial density had a significant positive influence on the average number of offspring produced, which ranged from 359 to 813 per female. The intrinsic rate of natural increase r m, which ranges from 1.10 to 1.19 day(-1), was neither influenced by the bacterial density, nor was the mean generation time T (5.12-5.25 days) and population doubling time (PDT) (0.64-0.59 days). The average lifespan of reproductive females, which ranged from 6.7 to 7.3 days, was positively correlated with bacterial density. A positive correlation between female body volume and bacterial density was recorded (R = 0.67) as well as a significant positive correlation between female body size and offspring production (R = 0.89) in hanging drops. Whether these data can be used to predict nematode yields in liquid culture was tested. The total female body volume calculated as the average female body volume × total number of parental females per millilitre 3 days after nematode inoculation was positively correlated (R = 0.72) with nematode yields. The total female body volume on process day 3 is thus a good indicator for the estimation of nematode yield at the end of the process (12-15 days post dauer juvenile (DJ) inoculation) in both Erlenmeyer flasks and bioreactors. With a mean deviation of 9467 DJs ml(-1), the error resembles approximately 5 % of the final DJ yields.
authors.affiliation: Faculty of Agricultural and Nutritional Sciences, Christian-Albrechts-University of Kiel, Hermann-Rodewald-Str. 4, 24118, Kiel, Germany.|e-nema GmbH, Klausdorfer Str. 28-36, 24223, Schwentinental, Germany.
authors.forename: Temesgen
authors.initials: T
authors.lastname: Addis
authors.affiliation: Department of Biology, Ghent University, K.L. Ledeganckstraat 35, B-9000, Ghent, Belgium.
authors.forename: Asmamaw
authors.initials: A
authors.lastname: Teshome
authors.affiliation: e-nema GmbH, Klausdorfer Str. 28-36, 24223, Schwentinental, Germany.
authors.forename: Olaf
authors.initials: O
authors.lastname: Strauch
authors.affiliation: Faculty of Agricultural and Nutritional Sciences, Christian-Albrechts-University of Kiel, Hermann-Rodewald-Str. 4, 24118, Kiel, Germany. ehlers@e-nema.de.|Department of Biology, Ghent University, K.L. Ledeganckstraat 35, B-9000, Ghent, Belgium. ehlers@e-nema.de.|e-nema GmbH, Klausdorfer Str. 28-36, 24223, Schwentinental, Germany. ehlers@e-nema.de.
authors.forename: Ralf-Udo
authors.initials: RU
authors.lastname: Ehlers
mesh_terms: D000818:Animals; D061046:Biological Control Agents; D049628:Body Size; D003470:Culture Media / Q000737:chemistry*; D005260:Female; D007313:Insecta / Q000469:parasitology; D000071421:Life History Traits; D008297:Male; D011157:Population Dynamics; D017168:Rhabditida / Q000254:growth & development* / Q000382:microbiology; D013559:Symbiosis; D020593:Xenorhabdus
chemical_list: D061046:Biological Control Agents; D003470:Culture Media
keywords: Biological control; Dauer juvenile yield; Endotokia matricida; Generation time; Hanging drop