title: Peripheral blood T- and B-cell immunophenotypic abnormalities in selected women with unexplained recurrent miscarriage.
abstract: We aimed to investigate if women with recurrent miscarriage disclosed abnormalities in the maturation and activation status of peripheral blood lymphocyte subsets. In a case control study, 24 women with recurrent miscarriage, 37 women with children but no history of miscarriage and 39 women without previous pregnancies were evaluated. Lymphocyte subsets were evaluated using three-colour flow-cytometry. Selected women with recurrent miscarriage had significantly higher absolute counts of central memory CD4+ T-cells, CD8+DR+ T-cells and memory non-switched B-cells than the control groups. Recurrent miscarriage may be associated with abnormalities of the maturation and activation status of peripheral blood T and B lymphocytes.
authors.affiliation: Clinical Immunology Department, Hospital General Universitario Gregorio Marañón, Madrid, Spain; Microbiology Department, Universidad Complutense de Madrid, Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain. Electronic address: javier.carbone@salud.madrid.org.
authors.forename: Javier
authors.initials: J
authors.lastname: Carbone
authors.affiliation: Clinical Immunology Department, Hospital General Universitario Gregorio Marañón, Madrid, Spain; Microbiology Department, Universidad Complutense de Madrid, Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Sarmiento
authors.affiliation: Clinical Immunology Department, Hospital General Universitario Gregorio Marañón, Madrid, Spain; Microbiology Department, Universidad Complutense de Madrid, Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Gallego
authors.affiliation: Clinical Immunology Department, Hospital General Universitario Gregorio Marañón, Madrid, Spain; Microbiology Department, Universidad Complutense de Madrid, Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain.
authors.forename: Nallibe
authors.initials: N
authors.lastname: Lanio
authors.affiliation: Clinical Immunology Department, Hospital General Universitario Gregorio Marañón, Madrid, Spain; Microbiology Department, Universidad Complutense de Madrid, Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain.
authors.forename: Joaquin
authors.initials: J
authors.lastname: Navarro
authors.affiliation: Clinical Immunology Department, Hospital General Universitario Gregorio Marañón, Madrid, Spain; Microbiology Department, Universidad Complutense de Madrid, Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain.
authors.forename: Sandra
authors.initials: S
authors.lastname: García
authors.affiliation: Clinical Immunology Department, Hospital General Universitario Gregorio Marañón, Madrid, Spain; Microbiology Department, Universidad Complutense de Madrid, Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Fernandez-Cruz
mesh_terms: D000026:Abortion, Habitual / Q000097:blood / Q000276:immunology*; D000328:Adult; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D007156:Immunologic Memory; D008213:Lymphocyte Activation; D011247:Pregnancy
keywords: Flow cytometry; T-cell activation; Unexplained recurrent miscarriage