title: Enzymatic saccharification of sugar cane bagasse by continuous xylanase and cellulase production from cellulomonas flavigena PR-22.
abstract: Cellulase (CMCase) and xylanase enzyme production and saccharification of sugar cane bagasse were coupled into two stages and named enzyme production and sugar cane bagasse saccharification. The performance of Cellulomonas flavigena (Cf) PR-22 cultured in a bubble column reactor (BCR) was compared to that in a stirred tank reactor (STR). Cells cultured in the BCR presented higher yields and productivity of both CMCase and xylanase activities than those grown in the STR configuration. A continuous culture with Cf PR-22 was run in the BCR using 1% alkali-pretreated sugar cane bagasse and mineral media, at dilution rates ranging from 0.04 to 0.22 1/h. The highest enzymatic productivity values were found at 0.08 1/h with 1846.4 ± 126.4 and 101.6 ± 5.6 U/L·h for xylanase and CMCase, respectively. Effluent from the BCR in steady state was transferred to an enzymatic reactor operated in fed-batch mode with an initial load of 75 g of pretreated sugar cane bagasse; saccharification was then performed in an STR at 55°C and 300 rpm for 90 h. The constant addition of fresh enzyme as well as the increase in time of contact with the substrate increased the total soluble sugar concentration 83% compared to the value obtained in a batch enzymatic reactor. This advantageous strategy may be used for industrial enzyme pretreatment and saccharification of lignocellulosic wastes to be used in bioethanol and chemicals production from lignocellulose. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:321-326, 2016.
authors.affiliation: Departamento de Biotecnología y Bioingeniería, Centro De Investigación y De Estudios Avanzados del Instituto Politécnico Nacional, Zacatenco, DF, 07300, México.
authors.forename: Óscar A
authors.initials: ÓA
authors.lastname: Rojas-Rejón
authors.affiliation: Departamento de Biotecnología y Bioingeniería, Centro De Investigación y De Estudios Avanzados del Instituto Politécnico Nacional, Zacatenco, DF, 07300, México.
authors.forename: Héctor M
authors.initials: HM
authors.lastname: Poggi-Varaldo
authors.affiliation: Departamento de Biotecnología y Bioingeniería, Centro De Investigación y De Estudios Avanzados del Instituto Politécnico Nacional, Zacatenco, DF, 07300, México.
authors.forename: Ana C
authors.initials: AC
authors.lastname: Ramos-Valdivia
authors.affiliation: Departamento de Biotecnología y Bioingeniería, Centro De Investigación y De Estudios Avanzados del Instituto Politécnico Nacional, Zacatenco, DF, 07300, México.
authors.forename: Teresa
authors.initials: T
authors.lastname: Ponce-Noyola
authors.affiliation: Departamento de Ingeniería Bioquímica, Escuela Nacional de Ciencias Biológicas del Instituto Politécnico Nacional, Prolongación Carpio y Plan De Ayala, Santo Tomás, DF, 11340, México.
authors.forename: Eliseo
authors.initials: E
authors.lastname: Cristiani-Urbina
authors.affiliation: Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Apdo. Postal 510-3, Cuernavaca, Morelos, 62250, México.
authors.forename: Alfredo
authors.initials: A
authors.lastname: Martínez
authors.affiliation: Departamento de Ciencia de los Alimentos. Centro de Investigación en Alimentación y Desarrollo, Carretera La Victoria Km 0.6, Hermosillo, 83304, Sonora, México.
authors.forename: Mayra
authors.initials: M
authors.lastname: de la Torre
mesh_terms: D019149:Bioreactors; D002480:Cellulase / Q000096:biosynthesis / Q000378:metabolism*; D040143:Cellulomonas / Q000166:cytology / Q000201:enzymology*; D002482:Cellulose / Q000096:biosynthesis* / Q000737:chemistry; D002498:Centrifugation; D043364:Endo-1,4-beta Xylanases / Q000096:biosynthesis / Q000378:metabolism*; D005285:Fermentation; D031786:Saccharum / Q000737:chemistry / Q000378:metabolism*
chemical_list: D002482:Cellulose; C027433:bagasse; D002480:Cellulase; D043364:Endo-1,4-beta Xylanases
keywords: cellulase; cellulomonas flavigena; saccharification; sugar cane bagasse; xylanase