title: Characterization of nuclear microsatellite markers for Rumex bucephalophorus (Polygonaceae) using 454 sequencing.
abstract: OBJECTIVE
  Nuclear microsatellite markers were developed in Rumex bucephalophorus subsp. canariensis (Polygonaceae) to investigate its genetic diversity and structure.
  
  
  RESULTS
  Sixteen polymorphic microsatellite markers were obtained using 454 next-generation sequencing with di-, tri-, and tetranucleotide repeats. The average number of alleles was 5.688 and 3.813 for R. bucephalophorus subsp. canariensis var. canariensis and var. fruticescens, respectively. Slightly higher levels of mean genetic diversity were found in var. canariensis (expected heterozygosity = 0.600) than in var. fruticescens (expected heterozygosity = 0.514). Cross-amplifications in related taxa within R. bucephalophorus showed good amplification and polymorphic patterns.
  
  
  CONCLUSIONS
  These 16 novel nuclear microsatellite markers are the first in the genus Rumex and may serve as valuable tools to carry out studies on genetic diversity and structure as well as progeny studies.
authors.affiliation: Departamento Biología Vegetal y Ecología, Universidad de Sevilla, Avda. Reina Mercedes, 41012 Seville, Spain.
authors.forename: Juan
authors.initials: J
authors.lastname: Viruel
authors.affiliation: Departamento Biología Vegetal y Ecología, Universidad de Sevilla, Avda. Reina Mercedes, 41012 Seville, Spain.
authors.forename: Pedro L
authors.initials: PL
authors.lastname: Ortiz
authors.affiliation: Departamento Biología Vegetal y Ecología, Universidad de Sevilla, Avda. Reina Mercedes, 41012 Seville, Spain.
authors.forename: Montserrat
authors.initials: M
authors.lastname: Arista
authors.affiliation: Departamento Biología Vegetal y Ecología, Universidad de Sevilla, Avda. Reina Mercedes, 41012 Seville, Spain.
authors.forename: María
authors.initials: M
authors.lastname: Talavera
keywords: Macaronesian; Mediterranean; Polygonaceae; Rumex bucephalophorus; heterocarpy; simple sequence repeat (SSR)