title: Co-localization of PTEN and E-cadherin in canine mammary hyperplasias and benign and malignant mammary tumors.
abstract: Fifty-four canine mammary lesions (15 hyperplasias, 7 adenomas and 32 carcinomas) were submitted to immunohistochemical analysis for the evaluation of PTEN and E-cadherin co-expression. Subjects bearing mammary carcinomas were also submitted to a 2-year follow-up study to compare immunohistochemical results with overall survival. All the hyperplastic samples stained positive for both markers, 100% of adenomas were positive for PTEN and 86% for E-cadherin, and 69% and 34% of carcinomas were positive for PTEN and E-cadherin, respectively. Statistical analysis showed a positive correlation between these two proteins both considering all (p b 0.01) or malignant tumors (p < 0.05). The female dogs bearing tumors positively-stained for both markers had a longer overall survival (p < 0.05) and absence of lymphatics invasion (p < 0.05). Simultaneous double immunofluorescence confirmed the co-localization of the two proteins in neoplastic cells. Results reported in this study confirm the tumor suppressor effect of these two molecules.
authors.affiliation: Department of Veterinary Sciences, University of Pisa, Italy; Institute of Research of Semiochemistry and Applied Ethology (IRSEA), Apt, France.
authors.forename: Pietro
authors.initials: P
authors.lastname: Asproni
authors.affiliation: Section of Veterinary Pathology, School of Veterinary Science, University of Liverpool, UK.
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Ressel
authors.affiliation: Department of Veterinary Sciences, University of Pisa, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Millanta
authors.affiliation: Department of Veterinary Sciences, University of Pisa, Italy.
authors.forename: Iacopo
authors.initials: I
authors.lastname: Vannozzi
authors.affiliation: Department of Veterinary Sciences, University of Pisa, Italy. Electronic address: alessandro.poli@unipi.it.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Poli
mesh_terms: D000236:Adenoma / Q000235:genetics / Q000662:veterinary; D000818:Animals; D015820:Cadherins / Q000235:genetics* / Q000378:metabolism; D002277:Carcinoma / Q000235:genetics / Q000662:veterinary; D004283:Dog Diseases / Q000235:genetics*; D004285:Dogs; D005260:Female; D005455:Fluorescent Antibody Technique / Q000662:veterinary; D005500:Follow-Up Studies; D015972:Gene Expression Regulation, Neoplastic; D006965:Hyperplasia / Q000235:genetics / Q000662:veterinary; D015674:Mammary Neoplasms, Animal / Q000235:genetics*; D051059:PTEN Phosphohydrolase / Q000235:genetics* / Q000378:metabolism; D014018:Tissue Distribution
chemical_list: D015820:Cadherins; D051059:PTEN Phosphohydrolase
keywords: Canine mammary hyperplasias; Canine mammary tumors; E-cadherin; Overall survival; PTEN