title: Selenomethionine, p-cyanophenylalanine pairs provide a convenient, sensitive, non-perturbing fluorescent probe of local helical structure.
abstract: The use of selenomethionine (MSe)-p-cyanophenylalanine (FCN) pairs to probe protein structure is demonstrated. MSe quenches FCN fluorescence via electron transfer. Both residues can be incorporated recombinantly or by peptide synthesis. Time-resolved and steady-state fluorescence measurements demonstrate that MSe-FCN pairs provide specific local probes of helical structure.
authors.affiliation: Department of Chemistry, Stony Brook University, Stony Brook, New York, 11794-3400, USA. daniel.raleigh@stonybrook.edu.
authors.forename: Ivan
authors.initials: I
authors.lastname: Peran
authors.affiliation: Department of Chemistry, Stony Brook University, Stony Brook, New York, 11794-3400, USA. daniel.raleigh@stonybrook.edu.
authors.forename: Matthew D
authors.initials: MD
authors.lastname: Watson
authors.affiliation: Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, Massachusetts 01605, USA.
authors.forename: Osman
authors.initials: O
authors.lastname: Bilsel
authors.affiliation: Department of Chemistry, Stony Brook University, Stony Brook, New York, 11794-3400, USA. daniel.raleigh@stonybrook.edu and Graduate Program in Biochemistry & Structural Biology, Stony Brook University, Stony Brook, New York, 11794-3400, USA.
authors.forename: Daniel P
authors.initials: DP
authors.lastname: Raleigh
mesh_terms: D000409:Alanine / Q000031:analogs & derivatives* / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D006860:Hydrogen Bonding; D056004:Molecular Dynamics Simulation; D009570:Nitriles / Q000737:chemistry*; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry*; D017433:Protein Structure, Secondary; D012645:Selenomethionine / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D010649:Phenylalanine / Q000031:analogs & derivatives
chemical_list: D000409:Alanine; D005456:Fluorescent Dyes; D009570:Nitriles; D010455:Peptides; D012645:Selenomethionine; C530634:p-cyanophenylalanine; D010649:Phenylalanine