title: Capillary electrochromatography using monoamine- and triamine-bonded silica nanoparticles as pseudostationary phases.
abstract: Monoamine- and triamine-bonded silica nanoparticles were prepared using 3-aminopropyltrimethoxysilane and N(1)-(3-trimethoxysilylpropyl)diethylenetriamine, respectively, and used as pseudostationary phases for capillary electrochromatography. The amine-bonded silica nanoparticles were tightly adsorbed on the inner wall of a capillary and generated fast electro-osmotic flow (2.59 × 10(-4) cm(2) V(-1) s(-1)) toward the anode in an electric field. The electro-osmotic velocities obtained with 20 nm triamine-bonded silica were three to five times larger than those generated by a fused silica capillary and two times faster than those for the commercial cationic polymer-modified capillary. Fast electro-osmotic flow enables rapid analysis. This method was applied to hydrophilic interaction chromatography (HILIC) mode separation of various samples including the size separation of glucose oligomer derivatives and the resolution of four nucleic acid bases.
authors.affiliation: Faculty of Pharmaceutical Sciences, Kinki University, 3-4-1, Kowakae, Higashi-Osaka, Osaka 577-8502, Japan.
authors.forename: Yuto
authors.initials: Y
authors.lastname: Takeda
authors.affiliation: Faculty of Pharmaceutical Sciences, Kinki University, 3-4-1, Kowakae, Higashi-Osaka, Osaka 577-8502, Japan.
authors.forename: Yuka
authors.initials: Y
authors.lastname: Hayashi
authors.affiliation: Faculty of Pharmaceutical Sciences, Kinki University, 3-4-1, Kowakae, Higashi-Osaka, Osaka 577-8502, Japan.
authors.forename: Naonori
authors.initials: N
authors.lastname: Utamura
authors.affiliation: Faculty of Pharmaceutical Sciences, Kinki University, 3-4-1, Kowakae, Higashi-Osaka, Osaka 577-8502, Japan.
authors.forename: Chise
authors.initials: C
authors.lastname: Takamoto
authors.affiliation: Faculty of Pharmaceutical Sciences, Kinki University, 3-4-1, Kowakae, Higashi-Osaka, Osaka 577-8502, Japan.
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Kinoshita
authors.affiliation: Faculty of Pharmaceutical Sciences, Kinki University, 3-4-1, Kowakae, Higashi-Osaka, Osaka 577-8502, Japan.
authors.forename: Sachio
authors.initials: S
authors.lastname: Yamamoto
authors.affiliation: Faculty of Pharmaceutical Sciences, Kinki University, 3-4-1, Kowakae, Higashi-Osaka, Osaka 577-8502, Japan.
authors.forename: Takao
authors.initials: T
authors.lastname: Hayakawa
authors.affiliation: Faculty of Pharmaceutical Sciences, Kinki University, 3-4-1, Kowakae, Higashi-Osaka, Osaka 577-8502, Japan. Electronic address: suzuki@phar.kindai.ac.jp.
authors.forename: Shigeo
authors.initials: S
authors.lastname: Suzuki
mesh_terms: D000327:Adsorption; D053801:Capillary Electrochromatography / Q000379:methods; D057927:Hydrophobic and Hydrophilic Interactions; D053758:Nanoparticles; D017646:Organosilicon Compounds / Q000737:chemistry*; D011073:Polyamines / Q000737:chemistry*; D011437:Propylamines / Q000737:chemistry*; D012821:Silanes / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: C575344:N-(3-trimethoxysilylpropyl)diethylenetriamine; D017646:Organosilicon Compounds; D011073:Polyamines; D011437:Propylamines; D012821:Silanes; C088294:3-aminopropyltrimethoxysilane; D012822:Silicon Dioxide
keywords: Amine-bonded silica; Capillary electrochromatography; Polyamine-bonded silica; Silica nanoparticles