title: Anticancer effect of eupatilin on glioma cells through inhibition of the Notch-1 signaling pathway.
abstract: Eupatilin, one of the major flavonoids in Artemisia asiatica Nakai (Asteraceae), has been reported to possess antitumor properties. However, thus far there have been no reports regarding the effects of eupatilin on glioma. Therefore, in the current study the effects of eupatilin on glioma and the underlying molecular mechanism were explored. The effect of eupatilin on cell viability was detected by the MTT assay. Cell invasion and migration were performed with Transwell assays and cell apoptosis was determined by flow cytometric analysis. Notch‑1 knockdown cells were established by transfection with Notch‑1 small interfering RNA (siRNA). The expression levels of Notch‑1 were detected by quantitative reverse transcription‑polymerase chain reaction and western blotting. The results of the present study indicated that eupatilin exhibits an anticancer effect on glioma cells. Eupatilin inhibited proliferation, reduced cell invasion and migration, and promoted the apoptosis of glioma cells. Additionally, it suppressed Notch‑1 expression. Knockdown of Notch‑1 by siRNA contributed to the inhibitory effect of eupatilin on proliferation and invasion of glioma cells. In conclusion, eupatilin had an inhibitory effect on proliferation, invasion and migration, and promoted apoptosis of glioma cells through suppression of the Notch‑1 signaling pathway. Therefore, eupatilin may have potential as an effective agent for the treatment of glioma.
authors.affiliation: Department of Electromyography, Tianjin Hospital, Tianjin 300211, P.R. China.
authors.forename: Yawei
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Infection Control, Hebei Chest Hospital, Shijiazhuang, Hebei 050048, P.R. China.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Hou
authors.affiliation: Basic Medical Institution, Shanghai Jiaotong University, Shanghai 200025, P.R. China.
authors.forename: Ming
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Orthopedics, Tianjin Hospital, Tianjin 300211, P.R. China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Basic Medical Institution, Shanghai Jiaotong University, Shanghai 200025, P.R. China.
authors.forename: Lan
authors.initials: L
authors.lastname: Sun
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D001932:Brain Neoplasms / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D015536:Down-Regulation / Q000187:drug effects; D005419:Flavonoids / Q000494:pharmacology*; D005910:Glioma / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D034741:RNA, Small Interfering / Q000378:metabolism; D051880:Receptors, Notch / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects*
chemical_list: D000970:Antineoplastic Agents; D005419:Flavonoids; D034741:RNA, Small Interfering; D051880:Receptors, Notch; C045325:eupatilin