title: Di- and Triterpenoids from the Leaves of Casearia balansae and Neurite Outgrowth Promoting Effects of PC12 Cells.
abstract: A bioassay-guided phytochemical investigation of the leaves of Casearia balansae led to the isolation of six new cucurbitane-type triterpenoid derivatives (balanterpenes A-F, 1-6) and four new clerdoane-type diterpenoids (balanterpenes G-J, 7-10). The structures of 1-10 were established on the basis of extensive analysis of NMR spectroscopic data, X-ray crystallography, and experimental and calculated electronic circular dichroism spectra. Compound 1 features a ring-expanded triterpenoid skeleton with the C-19 methyl involved in the ring formation, compound 6 possesses a rare hexanortriterpenoid scaffold, and compounds 7-10 may be four new diterpenoid artifacts presumably formed during the extraction and purification processes. Compounds 3 and 7-10 showed promoting effects on neurite outgrowth of PC12 cells with EC50 values in the range 2.9-10.0 μM.
authors.forename: Jing
authors.initials: J
authors.lastname: Xu
authors.forename: Jing
authors.initials: J
authors.lastname: Kang
authors.forename: Xiaocong
authors.initials: X
authors.lastname: Sun
authors.forename: Xiangrong
authors.initials: X
authors.lastname: Cao
authors.affiliation: College of Pharmacy, Xinjiang Medical University , Urumuqi 830011, People's Republic of China.
authors.forename: Kasimu
authors.initials: K
authors.lastname: Rena
authors.affiliation: Department of Biosystems and Biotechnology, College of Life Sciences and Biotechnology, Korea University , Seoul 136-713, Korea.
authors.forename: Dongho
authors.initials: D
authors.lastname: Lee
authors.forename: Quanhui
authors.initials: Q
authors.lastname: Ren
authors.forename: Shen
authors.initials: S
authors.lastname: Li
authors.affiliation: Graduate School of Pharmaceutical Sciences, Tohoku University , Sendai 980-8578, Japan.
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Ohizumi
authors.forename: Yuanqiang
authors.initials: Y
authors.lastname: Guo
mesh_terms: D000818:Animals; D037821:Casearia / Q000737:chemistry*; D045785:Diterpenes, Clerodane / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D015394:Molecular Structure; D016501:Neurites / Q000187:drug effects*; D019906:Nuclear Magnetic Resonance, Biomolecular; D016716:PC12 Cells; D018515:Plant Leaves / Q000737:chemistry; D051381:Rats; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*
chemical_list: D045785:Diterpenes, Clerodane; D004365:Drugs, Chinese Herbal; D014315:Triterpenes