title: 5-Azacytidine delivered by mesoporous silica nanoparticles regulates the differentiation of P19 cells into cardiomyocytes.
abstract: Heart disease is one of the deadliest diseases causing mortality due to the limited regenerative capability of highly differentiated cardiomyocytes. Stem cell-based therapy in tissue engineering is one of the most exciting and rapidly growing areas and raises promising prospects for cardiac repair. In this study, we have synthesized FITC-mesoporous silica nanoparticles (FMSNs) based on a sol-gel method (known as Stöber's method) as a drug delivery platform to transport 5-azacytidine in P19 embryonic carcinoma stem cells. The surfactant CTAB is utilized as a liquid crystal template to self-aggregate into micelles, resulting in the synthesis of MSNs. Based on the cell viability assay, treatment with FMSNs + 5-azacytidine resulted in much more significant inhibition of the proliferation than 5-azacytidine alone. To study the mechanism, we have tested the differentiation genes and cardiac marker genes in P19 cells and found that these genes have been up-regulated in P19 embryonic carcinoma stem cells treated with FMSNs + 5-azacytidine + poly(allylamine hydrochloride) (PAH), with the changes of histone modifications on the regulatory region. In conclusion, with FMSNs as drug delivery platforms, 5-azacytidine can be more efficiently delivered into stem cells and can be used to monitor and track the transfection process in situ to clarify their effects on stem cell functions and the differentiation process, which can serve as a promising tool in tissue engineering and other biomedical fields.
authors.affiliation: Department of Cardiology, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China. splu234@163.com.
authors.forename: Jin
authors.initials: J
authors.lastname: Cheng
authors.affiliation: Department of Anesthesiology, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Ding
authors.affiliation: Department of Ultrasound Diagnostics, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Oncology, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Deng
authors.affiliation: Department of Anesthesiology, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Yang
authors.affiliation: Department of Anesthesiology, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Tao
authors.affiliation: Department of Neonatology, West China Second University Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Haihong
authors.initials: H
authors.lastname: Lei
authors.affiliation: Department of Cardiology, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China. splu234@163.com.
authors.forename: Shaoping
authors.initials: S
authors.lastname: Lu
mesh_terms: D001374:Azacitidine / Q000737:chemistry / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects*; D045744:Cell Line, Tumor; D004337:Drug Carriers / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D032383:Myocytes, Cardiac / Q000378:metabolism*; D053758:Nanoparticles / Q000737:chemistry*; D009373:Neoplasms, Germ Cell and Embryonal / Q000378:metabolism*; D014411:Neoplastic Stem Cells / Q000378:metabolism*; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry / Q000494:pharmacology
chemical_list: D004337:Drug Carriers; D012822:Silicon Dioxide; D001374:Azacitidine