title: The overexpression of Thioredoxin-1 suppressing inflammation induced by methamphetamine in spleen.
abstract: BACKGROUND
  Methamphetamine (METH) is an addictive psychostimulant and has been shown to induce oxidative stress and inflammation in various tissues. Thioredoxin-1 (Trx-1) plays the roles in regulating redox and inhibiting inflammation. Whether Trx-1 is involved in METH-induced inflammation is still unknown.
  
  
  METHODS
  The present study was designed to investigate inflammatory factors in spleen of wild type and Trx-1 overexpression transgenic mice after METH treatment.
  
  
  RESULTS
  We found the mRNA level of Trx-1 was decreased and mRNA level of Trx-1 binding protein-2 (TBP-2) was increased. The mRNA levels of tumor necrosis factor-α (TNF-α), interferon-γ(IFN-γ), interleukin-2 (IL-2), T-bet and signal transducer and activators of transcription 4 (STAT 4) were increased and the mRNA levels of IL-10, GA-TA-binding protein-3 (GATA-3) and STAT 6 were decreased. Overexpression of Trx-1 reversed the above effects induced by METH.
  
  
  CONCLUSIONS
  The present study showed for the first time that Trx-1 overexpression suppressed the inflammation induced by METH.
authors.affiliation: Medical Faculty, Kunming University of Science and Technology, No.727 South Jingming Road, Kunming 650500, China.
authors.forename: Xiao-Li
authors.initials: XL
authors.lastname: Wu
authors.affiliation: Medical Faculty, Kunming University of Science and Technology, No.727 South Jingming Road, Kunming 650500, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Li
authors.affiliation: Medical Faculty, Kunming University of Science and Technology, No.727 South Jingming Road, Kunming 650500, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Li
authors.affiliation: Medical Faculty, Kunming University of Science and Technology, No.727 South Jingming Road, Kunming 650500, China.
authors.forename: Ling-Pin
authors.initials: LP
authors.lastname: Kong
authors.affiliation: Medical Faculty, Kunming University of Science and Technology, No.727 South Jingming Road, Kunming 650500, China.
authors.forename: Jiao-Long
authors.initials: JL
authors.lastname: Fang
authors.affiliation: Medical Faculty, Kunming University of Science and Technology, No.727 South Jingming Road, Kunming 650500, China.
authors.forename: Xiao-Shuang
authors.initials: XS
authors.lastname: Zhou
authors.affiliation: Medical Faculty, Kunming University of Science and Technology, No.727 South Jingming Road, Kunming 650500, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Li
authors.affiliation: Medical Faculty, Kunming University of Science and Technology, No.727 South Jingming Road, Kunming 650500, China.
authors.forename: Jin-Jing
authors.initials: JJ
authors.lastname: Jia
authors.affiliation: Medical Faculty, Kunming University of Science and Technology, No.727 South Jingming Road, Kunming 650500, China. Electronic address: jiebai662001@126.com.
authors.forename: Jie
authors.initials: J
authors.lastname: Bai
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000096:biosynthesis; D000697:Central Nervous System Stimulants / Q000009:adverse effects; D050990:GATA3 Transcription Factor / Q000096:biosynthesis; D007249:Inflammation / Q000139:chemically induced* / Q000235:genetics / Q000378:metabolism*; D007371:Interferon-gamma / Q000096:biosynthesis; D016753:Interleukin-10 / Q000096:biosynthesis; D007376:Interleukin-2 / Q000096:biosynthesis; D008694:Methamphetamine / Q000009:adverse effects*; D051379:Mice; D008822:Mice, Transgenic; D050791:STAT Transcription Factors / Q000096:biosynthesis; D013154:Spleen / Q000187:drug effects / Q000378:metabolism*; D020825:T-Box Domain Proteins / Q000096:biosynthesis; D013879:Thioredoxins / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis; D000098815:T-bet Transcription Factor
chemical_list: D002352:Carrier Proteins; D000697:Central Nervous System Stimulants; D050990:GATA3 Transcription Factor; C494748:Gata3 protein, mouse; D007376:Interleukin-2; D050791:STAT Transcription Factors; D020825:T-Box Domain Proteins; D000098815:T-bet Transcription Factor; D014409:Tumor Necrosis Factor-alpha; C445672:Txnip protein, mouse; D016753:Interleukin-10; D008694:Methamphetamine; D013879:Thioredoxins; D007371:Interferon-gamma
keywords: Inflammatory mediators; Methamphetamine; Thioredoxin-1