title: Effect of siRNA targeting HER2/neu on the proliferation and viability of prostate cancer PC-3M cells.
abstract: The aim of this study was to investigate the effect of a small interfering RNA (siRNA) targeting human epidermal growth factor receptor 2 (HER2/neu) on the proliferation and viability of prostate cancer PC-3M cells. Chemically synthesized siRNA targeting HER2/neu was transfected into PC-3M cells by using liposomes, and cells transfected with empty liposomes, a negative siRNA sequence, or nothing (untransfected) were used as controls. mRNA and protein levels of HER2/neu were detected using reverse transcription-polymerase chain reaction and western blot, respectively. The inhibitory action of HER2/neu siRNA on the in vitro growth of PC-3M cells was assessed by the cholecystokinin 8 assay and apoptosis was detected using flow cytometry. Cells transfected with HER2/neu siRNA showed decreased mRNA and protein levels of HER2/neu compared to control groups (P < 0.05). The survival rate of PC-3M cells decreased significantly after transfection with HER2/neu siRNA compared to that of untransfected cells (55.39 ± 1.60 and 81.42 ± 0.80%, respectively; P < 0.05). The apoptosis rate in cells transfected with HER2/neu siRNA was quite high (45.60 ± 0.70%) compared to that of blank control, empty liposome, and negative siRNA sequence groups (P < 0.05). In conclusion, siRNA targeting HER2/neu inhibits HER2/neu expression in PC-3M cells, resulting in an inhibition in proliferation and an induction of apoptosis.
authors.affiliation: Department of Urology, Lu'an Affiliated Hospital of Anhui Medical University, Lu'an, China.
authors.forename: C Y
authors.initials: CY
authors.lastname: Liu
authors.affiliation: Department of Urology, Lu'an Affiliated Hospital of Anhui Medical University, Lu'an, China.
authors.forename: P C
authors.initials: PC
authors.lastname: Xu
authors.affiliation: Department of Urology, Lu'an Affiliated Hospital of Anhui Medical University, Lu'an, China.
authors.forename: D G
authors.initials: DG
authors.lastname: Chen
authors.affiliation: Department of Urology, Lu'an Affiliated Hospital of Anhui Medical University, Lu'an, China.
authors.forename: X H
authors.initials: XH
authors.lastname: Fan
authors.affiliation: Department of Urology, Fujian Medical University Union Hospital, Fuzhou, China.
authors.forename: M Q
authors.initials: MQ
authors.lastname: Li
authors.affiliation: Department of Urology, Fujian Medical University Union Hospital, Fuzhou, China.
authors.forename: X
authors.initials: X
authors.lastname: Yang
authors.affiliation: Institute of Urology, Zhejiang Cancer Hospital, Hangzhou, China.
authors.forename: Y P
authors.initials: YP
authors.lastname: Xu
mesh_terms: D017209:Apoptosis; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002470:Cell Survival / Q000235:genetics; D002478:Cells, Cultured; D015870:Gene Expression; D006801:Humans; D008297:Male; D011471:Prostatic Neoplasms / Q000235:genetics*; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics*; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics*; D014162:Transfection
chemical_list: D012333:RNA, Messenger; D034741:RNA, Small Interfering; D018719:Erb-b2 Receptor Tyrosine Kinases