title: Neuropeptide Y directly affects ovarian cell proliferation and apoptosis.
abstract: The effects of neuropeptide Y (NPY; 0, 10, 100 and 1000 ng/mL) on the expression of PCNA, bax and p53 were examined by immunocytochemistry in porcine luteinized granulosa cells. NPY inhibited proliferation as well as promoted apoptosis and accumulation of p53 in the cells. This is the first report to demonstrate the direct action of NPY on ovarian cell proliferation and apoptosis. The results of the study suggest that the effect is mediated by transcription factor p53.
authors.affiliation: Constantine The Philosopher University in Nitra, Nitra, Slovakia; Research Institute of Animal Production Nitra, Lužianky, Slovakia; King Saud University, Department of Zoology, College of Science, Riyadh, Saudi Arabia. Electronic address: sirotkin@vuzv.sk.
authors.forename: Alexander V
authors.initials: AV
authors.lastname: Sirotkin
authors.affiliation: Constantine The Philosopher University in Nitra, Nitra, Slovakia.
authors.forename: Diana
authors.initials: D
authors.lastname: Kardošová
authors.affiliation: King Saud University, Department of Zoology, College of Science, Riyadh, Saudi Arabia.
authors.forename: Saleh Hamad
authors.initials: SH
authors.lastname: Alwasel
authors.affiliation: King Saud University, Department of Zoology, College of Science, Riyadh, Saudi Arabia.
authors.forename: Abdel Halim
authors.initials: AH
authors.lastname: Harrath
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D049109:Cell Proliferation / Q000187:drug effects* / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D006107:Granulosa Cells / Q000187:drug effects* / Q000502:physiology; D009478:Neuropeptide Y / Q000008:administration & dosage / Q000494:pharmacology*; D018809:Proliferating Cell Nuclear Antigen / Q000235:genetics / Q000378:metabolism; D013552:Swine; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000235:genetics / Q000378:metabolism
chemical_list: D009478:Neuropeptide Y; D018809:Proliferating Cell Nuclear Antigen; D016159:Tumor Suppressor Protein p53; D051028:bcl-2-Associated X Protein
keywords: Apoptosis; Neuropeptide Y; Ovary; Proliferation; Transcription factor p53