title: Protein Arginine Methyltransferase 6 Involved in Germ Cell Viability during Spermatogenesis and Down-Regulated by the Androgen Receptor.
abstract: Androgens and the androgen receptor (AR) are of great importance to spermatogenesis and male fertility. AR knockout (ARKO) mice display a complete insensitivity to androgens and male infertility; however, the exact molecular mechanism for this effect remains unclear. In this study, we found that the expression levels of Prmt6 mRNA and protein were significantly up-regulated in the testes of ARKO mice compared to wild type (WT) mice. PRMT6 was principally localized to the nucleus of spermatogonia and spermatocytes by immunofluorescence staining. Furthermore, luciferase assay data showed that AR together with testosterone treatment suppressed Prmt6 transcription via binding to the androgen-responsive element (ARE) of the Prmt6 promoter. Moreover, knockdown of Prmt6 suppressed germ cells migration and promoted apoptosis. In addition, both of these cellular activities could not be enhanced by testosterone treatment. Taken together, these data indicate that PRMT6, which was down-regulated by AR and influenced cell migration and apoptosis of germ cells, could play a potentially important role in spermatogenesis.
authors.affiliation: Department of Physiology, Shantou University Medical College, Shantou 515041, China. lmnhunan@126.com.
authors.forename: Manling
authors.initials: M
authors.lastname: Luo
authors.affiliation: Department of Physiology, Shantou University Medical College, Shantou 515041, China. 15096032050@163.com.|Guangdong and Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Institute of Urology, Peking University Shenzhen Hospital, Shenzhen PKU-HKUST Medical Center, Shenzhen 518036, China. 15096032050@163.com.
authors.forename: Yuchi
authors.initials: Y
authors.lastname: Li
authors.affiliation: Guangdong and Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Institute of Urology, Peking University Shenzhen Hospital, Shenzhen PKU-HKUST Medical Center, Shenzhen 518036, China. guohuan201405@163.com.|Department of Surgery, Guangzhou Medical University, Guangzhou 510182, China. guohuan201405@163.com.
authors.forename: Huan
authors.initials: H
authors.lastname: Guo
authors.affiliation: Guangdong and Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Institute of Urology, Peking University Shenzhen Hospital, Shenzhen PKU-HKUST Medical Center, Shenzhen 518036, China. linshouren198710@126.com.
authors.forename: Shouren
authors.initials: S
authors.lastname: Lin
authors.affiliation: Guangdong and Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Institute of Urology, Peking University Shenzhen Hospital, Shenzhen PKU-HKUST Medical Center, Shenzhen 518036, China. chenjianbo8090@163.com.|Department of Surgery, Anhui Medical University, Hefei 230032, China. chenjianbo8090@163.com.
authors.forename: Jianbo
authors.initials: J
authors.lastname: Chen
authors.affiliation: Guangdong and Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Institute of Urology, Peking University Shenzhen Hospital, Shenzhen PKU-HKUST Medical Center, Shenzhen 518036, China. mq@whu.edu.cn.
authors.forename: Qian
authors.initials: Q
authors.lastname: Ma
authors.affiliation: Guangdong and Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Institute of Urology, Peking University Shenzhen Hospital, Shenzhen PKU-HKUST Medical Center, Shenzhen 518036, China. guyl2418@Chinaren.com.
authors.forename: Yanli
authors.initials: Y
authors.lastname: Gu
authors.affiliation: Guangdong and Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Institute of Urology, Peking University Shenzhen Hospital, Shenzhen PKU-HKUST Medical Center, Shenzhen 518036, China. 13510857100@126.com.
authors.forename: Zhimao
authors.initials: Z
authors.lastname: Jiang
authors.affiliation: Guangdong and Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Institute of Urology, Peking University Shenzhen Hospital, Shenzhen PKU-HKUST Medical Center, Shenzhen 518036, China. guiyaoting2007@aliyun.com.
authors.forename: Yaoting
authors.initials: Y
authors.lastname: Gui
mesh_terms: D000818:Animals; D017209:Apoptosis; D019556:COS Cells; D002465:Cell Movement; D002470:Cell Survival; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D005786:Gene Expression Regulation; D008297:Male; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D021381:Protein Transport; D011484:Protein-Arginine N-Methyltransferases / Q000235:genetics / Q000378:metabolism*; D011944:Receptors, Androgen / Q000502:physiology*; D015398:Signal Transduction; D013091:Spermatogenesis; D013094:Spermatozoa / Q000502:physiology*; D013737:Testis / Q000201:enzymology; D013739:Testosterone / Q000502:physiology
chemical_list: D011944:Receptors, Androgen; D013739:Testosterone; C577312:PRMT6 protein, mouse; D011484:Protein-Arginine N-Methyltransferases
keywords: PRMT6; androgen receptor; apoptosis; migration; testes