title: MicroRNA-764-3p regulates 17β-estradiol synthesis of mouse ovarian granulosa cells by targeting steroidogenic factor-1.
abstract: Previous studies have reported that microRNA-764-3p (miR-764-3p) is one of the most up-regulated microRNAs (miRNAs) in TGF-β1-stimulated mouse ovarian granulosa cells. However, little is known about the roles and mechanisms of miR-764-3p in granulosa cell function during follicular development. In this study, we found that overexpression of miR-764-3p inhibited 17β-estradiol (E2) synthesis of granulosa cells through directly targeting steroidogenic factor-1 (SF-1). MiR-764-3p inhibited SF-1 by affecting its messenger RNA (mRNA) stability, which subsequently suppressed the expression levels of Cyp19a1 gene (aromatase, a downstream target of SF-1). In addition, SF-1 was involved in regulation of miR-764-3p-mediated Cyp19a1 expression in granulosa cells which contributed, at least partially, to the effects of miR-764-3p on granulosa cell E2 release. These results suggest that miR-764-3p functions to decrease steroidogenesis by targeting SF-1, at least in part, through inactivation of Cyp19a1. Taken together, our data provide mechanistic insights into the roles of miR-764-3p on E2 synthesis. Understanding of potential miRNAs affecting estrogen synthesis will help to diagnose and treat steroid-related diseases.
authors.affiliation: Department of Reproduction Health and Infertility, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, People's Republic of China.|China-Canada-New Zealand Joint Laboratory of Maternal and Fetal Medicine, Chongqing Medical University, Chongqing, 400016, People's Republic of China.
authors.forename: Lianlian
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Gynecology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, People's Republic of China.
authors.forename: Cong
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Obstetrics, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, People's Republic of China.|China-Canada-New Zealand Joint Laboratory of Maternal and Fetal Medicine, Chongqing Medical University, Chongqing, 400016, People's Republic of China.
authors.forename: Rong
authors.initials: R
authors.lastname: Li
authors.affiliation: Department of Gynecology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, People's Republic of China.
authors.forename: Youlin
authors.initials: Y
authors.lastname: Deng
authors.affiliation: Department of Medical Records, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, People's Republic of China.
authors.forename: Yixin
authors.initials: Y
authors.lastname: Tan
authors.affiliation: Department of Obstetrics, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, People's Republic of China.|China-Canada-New Zealand Joint Laboratory of Maternal and Fetal Medicine, Chongqing Medical University, Chongqing, 400016, People's Republic of China.
authors.forename: Chao
authors.initials: C
authors.lastname: Tong
authors.affiliation: Department of Obstetrics, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, People's Republic of China. qihongboqi@sina.com.|China-Canada-New Zealand Joint Laboratory of Maternal and Fetal Medicine, Chongqing Medical University, Chongqing, 400016, People's Republic of China. qihongboqi@sina.com.
authors.forename: Hongbo
authors.initials: H
authors.lastname: Qi
mesh_terms: D000818:Animals; D001141:Aromatase / Q000096:biosynthesis*; D049109:Cell Proliferation; D002478:Cells, Cultured; D004958:Estradiol / Q000096:biosynthesis* / Q000378:metabolism; D005260:Female; D006107:Granulosa Cells / Q000378:metabolism*; D051379:Mice; D008813:Mice, Inbred ICR; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics*; D054339:Steroidogenic Factor 1 / Q000037:antagonists & inhibitors* / Q000235:genetics
chemical_list: C576122:MIRN764 microRNA, mouse; D035683:MicroRNAs; D054339:Steroidogenic Factor 1; C515058:steroidogenic factor 1, mouse; D004958:Estradiol; D001141:Aromatase
keywords: 17β-estradiol; Granulosa cell; SF-1; miR-764-3p