title: Qualitative and Quantitative Analysis of Histone Deacetylases in Kidney Tissue Sections.
abstract: Fluorescent microscope imaging technologies are increasing in their applications and are being used on a wide scale. However methods used to quantify the level of fluorescence intensity are often not utilized-perhaps given the result may be immediately seen, quantification of the data may not seem necessary. However there are a number of reasons given to quantify fluorescent images including the importance of removing potential bias in the data upon observation as well as quantification of large numbers of images gives statistical power to detect subtle changes in experiments. In addition discreet localization of a protein could be detected without selection bias that may not be detectable by eye. Such data will be deemed useful when detecting the levels of HDAC enzymes within cells in order to develop more effective HDAC inhibitor compounds for use against multiple diseased states. Hence, we discuss a methodology devised to analyze fluorescent images using Image J to detect the mean fluorescence intensity of the 11 metal-dependent HDAC enzymes using murine kidney tissue sections as an example.
authors.affiliation: Epigenomic Medicine, Baker IDI Heart & Diabetes Institute, 75 Commercial Road, Melbourne, VIC, 3004, Australia.|Department of Pathology, University of Melbourne, Melbourne, VIC, Australia.
authors.forename: Katherine
authors.initials: K
authors.lastname: Ververis
authors.affiliation: Epigenomic Medicine, Baker IDI Heart & Diabetes Institute, 75 Commercial Road, Melbourne, VIC, 3004, Australia.
authors.forename: Selly
authors.initials: S
authors.lastname: Marzully
authors.affiliation: Department of Pharmacology, Monash University, Melbourne, VIC, Australia.
authors.forename: Chrishan S
authors.initials: CS
authors.lastname: Samuel
authors.affiliation: Department of Nephrology, The Royal Melbourne Hospital, Melbourne, VIC, Australia.|Department of Medicine, University of Melbourne, Melbourne, VIC, Australia.
authors.forename: Tim D
authors.initials: TD
authors.lastname: Hewitson
authors.affiliation: Epigenomic Medicine, Baker IDI Heart & Diabetes Institute, 75 Commercial Road, Melbourne, VIC, 3004, Australia. Tom.Karagiannis@bakeridi.edu.au.|Department of Pathology, University of Melbourne, Melbourne, VIC, Australia. Tom.Karagiannis@bakeridi.edu.au.
authors.forename: Tom C
authors.initials: TC
authors.lastname: Karagiannis
mesh_terms: D000818:Animals; D006655:Histone Deacetylases / Q000378:metabolism*; D006801:Humans; D007090:Image Interpretation, Computer-Assisted; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry / Q000379:methods*; D007668:Kidney / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D012984:Software
chemical_list: D006655:Histone Deacetylases
keywords: Fluorescence microscopy; Histone deacetylases; Image J analysis; Immunofluorescence staining; Kidney tissue