title: Quantitative proteomic analysis of Dunaliella salina upon acute arsenate exposure.
abstract: Dunaliella salina is resistant to arsenic (As) and can accumulate a large amount of this highly toxic metalloid in cells. To study the mechanisms of As tolerance, a label-free, LC-MS/MS-based proteomic approach was applied for the first time to identify and quantify differentially expressed proteins from D. salina exposed to 11.2 mg L(-1) arsenate (As(V)) for 72 h. The intracellular As content reached 19.8 mg kg(-1), leading to a significant increase of lipid peroxidation in cells and a 7.4% growth reduction of this microalga. Sixty-five proteins were differentially expressed (p < 0.05), with 45 significantly induced and 20 declined. These proteins were involved in energy metabolism, protein synthesis and folding, ROS scavenging and defense, phosphate transport and membrane trafficking, and amino acid synthesis. Taken together, this study provides novel insights on the As(V) detoxification in D. salina.
authors.affiliation: College of Resources and Environmental Sciences, Jiangsu Provincial Key Laboratory of Marine Biology, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: yingge711@njau.edu.cn.
authors.forename: Ying
authors.initials: Y
authors.lastname: Ge
authors.affiliation: Ottawa Institute of Systems Biology, Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine, University of Ottawa, Ottawa K1H 8M5, Canada.
authors.forename: Zhibin
authors.initials: Z
authors.lastname: Ning
authors.affiliation: College of Resources and Environmental Sciences, Jiangsu Provincial Key Laboratory of Marine Biology, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Ya
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Resources and Environmental Sciences, Jiangsu Provincial Key Laboratory of Marine Biology, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Yanheng
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Demonstration Laboratory of Proteomics Research, Laboratory Centre of Life Science, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Chunhua
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Ottawa Institute of Systems Biology, Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine, University of Ottawa, Ottawa K1H 8M5, Canada. Electronic address: dfigeys@uottawa.ca.
authors.forename: Daniel
authors.initials: D
authors.lastname: Figeys
mesh_terms: D001149:Arsenates / Q000378:metabolism / Q000633:toxicity*; D002853:Chromatography, Liquid; D004784:Environmental Monitoring / Q000379:methods*; D015227:Lipid Peroxidation / Q000187:drug effects; D058086:Microalgae / Q000187:drug effects* / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D040901:Proteomics / Q000379:methods*; D053719:Tandem Mass Spectrometry; D016823:Volvocida / Q000187:drug effects* / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000378:metabolism / Q000633:toxicity*
chemical_list: D001149:Arsenates; D014874:Water Pollutants, Chemical
keywords: Arsenic; Dunaliella; LC-MS/MS; Label-free; Proteomics