title: Histone H2AX and the small RNA pathway modulate both non-homologous end-joining and homologous recombination in plants.
abstract: Using a zinc finger nuclease (ZFN) that creates a site-specific DNA double-strand break (DSB) at the endogenous ADH1 locus, we provide genetic evidence that histone H2AX and DSB-induced small RNAs modulate DNA repair by both non-homologous end-joining (NHEJ) and homologous recombination (HR) in the model plant Arabidopsis thaliana.
authors.affiliation: Department of Biology, East Carolina University, Greenville, NC 27858, USA. Electronic address: qiy@ecu.edu.
authors.forename: Yiping
authors.initials: Y
authors.lastname: Qi
authors.affiliation: Department of Genetics, Cell Biology & Development and Center for Genome Engineering, University of MN, Minneapolis, MN 55455, USA; Department of Biotechnology, School of Life Sciences and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Genetics, Cell Biology & Development and Center for Genome Engineering, University of MN, Minneapolis, MN 55455, USA.
authors.forename: Joshua A
authors.initials: JA
authors.lastname: Baller
authors.affiliation: Department of Genetics, Cell Biology & Development and Center for Genome Engineering, University of MN, Minneapolis, MN 55455, USA.
authors.forename: Daniel F
authors.initials: DF
authors.lastname: Voytas
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D053903:DNA Breaks, Double-Stranded; D059766:DNA End-Joining Repair; D004260:DNA Repair; D006657:Histones / Q000235:genetics / Q000378:metabolism*; D059765:Homologous Recombination; D009154:Mutation; D011523:Protoplasts / Q000502:physiology; D018749:RNA, Plant / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D006657:Histones; D018749:RNA, Plant
keywords: Arabidopsis thaliana; DNA double-strand break (DSB) repair; H2AX; Small RNA; Zinc finger nuclease (ZFN)