title: Cancer Cell Targeting Using Folic Acid/Anti-HER2 Antibody Conjugated Fluorescent CdSe/CdS/ZnS-MPA and CdTe-MSA Quantum Dots.
abstract: CdSe/CdS/ZnS and CdTe quantum dots (QDs) were synthesized by successive ion layer adsorption and reaction (SILAR) technique and direct aqueous synthesis respectively using thiol stabilizers. Synthesized CdSe/CdS/ZnS and CdTe QDs stabilized with 3-mercaptopropionic acid (MPA) and mercaptosuccinic acid (MSA) were used as fluorescent labels after conjugation with folic acid (FA) and anti-HER2 antibodies. Photoluminescence quantum yield of folated CdSe/CdS/ZnS-MPA and CdTe-MSA QDs was 59% and 77% than that of non-folated hydrophilic QDs. The folate receptor-mediated delivery of folic acid-conjugated CdTe-MSA and CdSe/CdS/ZnS-MPA QDs showed higher cellular internalization as observed by confocal laser scanning microscopic studies. Folated and non-folated CdTe-MSA QDs were highly toxic and exhibited only 10% cell viability as compared to > 80% cell viability with CdSe/CdS/ZnS-MPA QDs over the concentration ranging from 3.38 to 50 pmoles. Immunohistochemistry (IHC) results of human breast cancer tissue samples showed positive results with anti-HER2 antibody conjugated CdSe/CdS/ZnS-MPA QDs with better sensitivity and specificity as compared to conventional IHC analysis using diaminobenzedene staining.
authors.forename: Gurpal
authors.initials: G
authors.lastname: Singh
authors.forename: Manoj
authors.initials: M
authors.lastname: Kumar
authors.forename: Udit
authors.initials: U
authors.lastname: Soni
authors.forename: Vikas
authors.initials: V
authors.lastname: Arora
authors.forename: Vivek
authors.initials: V
authors.lastname: Bansal
authors.forename: Dikshi
authors.initials: D
authors.lastname: Gupta
authors.forename: Madhusudan
authors.initials: M
authors.lastname: Bhat
authors.forename: Amit K
authors.initials: AK
authors.lastname: Dinda
authors.forename: Sameer
authors.initials: S
authors.lastname: Sapra
authors.forename: Harpal
authors.initials: H
authors.lastname: Singh
mesh_terms: D015097:3-Mercaptopropionic Acid; D000911:Antibodies, Monoclonal / Q000737:chemistry*; D001943:Breast Neoplasms / Q000000981:diagnostic imaging*; D019187:Cadmium Compounds / Q000737:chemistry; D005453:Fluorescence; D005492:Folic Acid / Q000737:chemistry*; D006801:Humans; D018613:Microscopy, Confocal; D045663:Quantum Dots / Q000737:chemistry*; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000037:antagonists & inhibitors*; D013194:Staining and Labeling; D013440:Sulfides
chemical_list: D015097:3-Mercaptopropionic Acid; D000911:Antibodies, Monoclonal; D019187:Cadmium Compounds; D005492:Folic Acid; D018719:Erb-b2 Receptor Tyrosine Kinases; D013440:Sulfides; C508053:ERBB2 protein, human