title: Characterization and Immunogenicity of Outer Membrane Vesicles from Brucella abortus.
abstract: Bovine brucellosis is a worldwide spread zoonotic disease. The objectives of this study were characterization of outer membrane vesicles from B. abortus and to evaluate their immunogenicity in mice. For this purpose, OMVs were derived from B. abortus strain 99 using ultracentrifugation method. Isolated OMVs were characterized by sodium dodecyl sulfate polyacrylamide gel electrophoresis and transmission electron microscopy which revealed spherical 20-300 nm structures rich in proteins. OMVs also showed immuno-reactivity with mice antisera in Western blot. Further, indirect ELISA showed specific and high-titer immune responses against the antigens present in OMVs suggesting their potential for a safe acellular vaccine candidate.
authors.affiliation: a School of Animal Biotechnology , Guru Angad Dev Veterinary and Animal Sciences University , Ludhiana , India.
authors.forename: Gagandeep
authors.initials: G
authors.lastname: Kaur
authors.affiliation: a School of Animal Biotechnology , Guru Angad Dev Veterinary and Animal Sciences University , Ludhiana , India.
authors.forename: Satparkash
authors.initials: S
authors.lastname: Singh
authors.affiliation: a School of Animal Biotechnology , Guru Angad Dev Veterinary and Animal Sciences University , Ludhiana , India.
authors.forename: B V
authors.initials: BV
authors.lastname: Sunil Kumar
authors.affiliation: a School of Animal Biotechnology , Guru Angad Dev Veterinary and Animal Sciences University , Ludhiana , India.
authors.forename: Kanika
authors.initials: K
authors.lastname: Mahajan
authors.affiliation: a School of Animal Biotechnology , Guru Angad Dev Veterinary and Animal Sciences University , Ludhiana , India.
authors.forename: Ramneek
authors.initials: R
authors.lastname: Verma
mesh_terms: D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D001428:Bacterial Vaccines / Q000737:chemistry / Q000276:immunology; D002003:Brucella abortus / Q000166:cytology* / Q000276:immunology*; D022162:Cytoplasmic Vesicles / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D015151:Immunoblotting; D000071497:Immunogenicity, Vaccine; D051379:Mice; D008854:Microscopy, Electron
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines
keywords: Brucella abortus; OMVs; SDS-PAGE; Western blotting; electron microscopy; indirect ELISA