title: Microbial community structure associated with the high loading anaerobic codigestion of olive mill and abattoir wastewaters.
abstract: The effect of increasing the organic loading rates (OLRs) on the performance of the anaerobic codigestion of olive mill (OMW) and abattoir wastewaters (AW) was investigated under mesophilic and thermophilic conditions. The structure of the microbial community was also monitored. Increasing OLR to 9g of chemical oxygen demand (COD) L(-1)d(-1) affected significantly the biogas yield and microbial diversity at 35°C. However, at 55°C digester remained stable until OLR of 12g of CODL(-1)d(-1) with higher COD removal (80%) and biogas yield (0.52Lg(-1) COD removed). Significant differences in the bacterial communities were detected between mesophilic and thermophilic conditions. The dominant phyla detected in the digester at both phases were the Firmicutes, Actinobacteria, Bacteroidetes, Synergistetes and Spirochaete. However, Verrucomicrobia, Proteobacteria and the candidate division BRC1 were only detected at thermophilic conditions. The Methanobacteriales and the Thermoplasmales were found as a high predominant archaeal member in the anaerobic sludge.
authors.affiliation: Université de Carthage, LR: LETMi, INSAT, B.P. 676, 1080 Tunis, Tunisia; Université de Tunis El Manar, ISSBAT, 9, Avenue Zouhaïer Essafi, 1006 Tunis, Tunisia.
authors.forename: Hana
authors.initials: H
authors.lastname: Gannoun
authors.affiliation: Université de Carthage, LR: LETMi, INSAT, B.P. 676, 1080 Tunis, Tunisia.
authors.forename: Ilhem
authors.initials: I
authors.lastname: Omri
authors.affiliation: Faculté des Sciences de Bizerte, Unité de Toxicologie Végétale et Biologie moléculaire des micro-organismes (UR11-ES-32), Tunisia.
authors.forename: Rakia
authors.initials: R
authors.lastname: Chouari
authors.affiliation: Université de Carthage, LR: LETMi, INSAT, B.P. 676, 1080 Tunis, Tunisia.
authors.forename: Eltaief
authors.initials: E
authors.lastname: Khelifi
authors.affiliation: Université de Carthage, LR: LETMi, INSAT, B.P. 676, 1080 Tunis, Tunisia.
authors.forename: Sajiaa
authors.initials: S
authors.lastname: Keskes
authors.affiliation: INRA U050, LBE, Avenue des Étangs, F-11100 Narbonne, France.
authors.forename: Jean-Jacques
authors.initials: JJ
authors.lastname: Godon
authors.affiliation: Université de Carthage, LR: LETMi, INSAT, B.P. 676, 1080 Tunis, Tunisia.
authors.forename: Moktar
authors.initials: M
authors.lastname: Hamdi
authors.affiliation: Université d'Evry Val d'Essonne (UEVE), Evry, France; CEA, Institut de Génomique, Genoscope, Evry, France.
authors.forename: Abdelghani
authors.initials: A
authors.lastname: Sghir
authors.affiliation: Université de Carthage, LR: LETMi, INSAT, B.P. 676, 1080 Tunis, Tunisia. Electronic address: hassibbouallagui@yahoo.fr.
authors.forename: Hassib
authors.initials: H
authors.lastname: Bouallagui
mesh_terms: D000003:Abattoirs; D000693:Anaerobiosis; D001105:Archaea / Q000378:metabolism*; D001419:Bacteria / Q000378:metabolism*; D056804:Biofuels; D057919:Biological Oxygen Demand Analysis; D019149:Bioreactors / Q000382:microbiology; D005232:Fatty Acids, Volatile / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D031658:Olea / Q000737:chemistry*; D009930:Organic Chemicals / Q000032:analysis; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D012722:Sewage / Q000382:microbiology; D014865:Waste Disposal, Fluid / Q000379:methods*; D062065:Wastewater / Q000737:chemistry*
chemical_list: D056804:Biofuels; D005232:Fatty Acids, Volatile; D009930:Organic Chemicals; D012722:Sewage; D062065:Wastewater
keywords: Anaerobic digestion; Microbial diversity; Olive mill wastewater; Temperature; Upflow anaerobic filter