title: Fibroblast Growth Factor 21 Suppresses Adipogenesis in Pig Intramuscular Fat Cells.
abstract: Fibroblast growth factor 21 (FGF21) plays an important role in the treatment of disease associated with muscle insulin resistance which is characterized by various factors, such as intramuscular triglyceride (IMT) content. Studies have also shown that FGF21 inhibits triglyceride synthesis in vivo. However, the precise mechanism whereby FGF21 regulates triglyceride metabolism in intramuscular fat (IMF), which may influence the muscle insulin sensitivity, is not clearly understood. In order to understand the role of FGF21 in IMF deposition, we performed FGF21 overexpression in IMF cells by stable transfection. Our results showed that FGF21 inhibited the key adipogenesis gene mRNA expression of peroxisome proliferator-activated receptor gamma (PPARG), CCAAT/enhancer-binding protein (CEBP) family by reducing lysine-specific demethylase 1 (LSD1) expression which led to significant decline in lipid accumulation, and the result was confirmed by Western blot. Moreover, triggered by FGF21, parts of the adipokines--fatty acid-binding protein 4 (FABP4), glucose transporter 4 (GLUT4), adiponectin (ADIPOQ), and perilipin (PLIN1)--were also down-regulated. Furthermore, FGF21 gene expression was suppressed by transcription factor CEBP beta (CEBPB) which contributed strongly to triglyceride synthesis. Taken together, our study is the first to experimentally demonstrate FGF21 emerging as an efficient blockade of adipogenesis in IMF, thus also providing a new understanding of the mechanism whereby FGF21 improves insulin sensitivity.
authors.affiliation: Key Laboratory of Agriculture Animal Genetics, Breeding and Reproduction, College of Animal Science, Huazhong Agricultural University, Wuhan 430070, China. ylwang@webmail.hzau.edu.cn.
authors.forename: Yongliang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Laboratory of Agriculture Animal Genetics, Breeding and Reproduction, College of Animal Science, Huazhong Agricultural University, Wuhan 430070, China. XinyiLiu9@gmail.com.
authors.forename: Xinyi
authors.initials: X
authors.lastname: Liu
authors.affiliation: Key Laboratory of Agriculture Animal Genetics, Breeding and Reproduction, College of Animal Science, Huazhong Agricultural University, Wuhan 430070, China. mnhouliming@gmail.com.
authors.forename: Liming
authors.initials: L
authors.lastname: Hou
authors.affiliation: Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. wuwangjun2012@njau.edu.cn.
authors.forename: Wangjun
authors.initials: W
authors.lastname: Wu
authors.affiliation: Key Laboratory of Agriculture Animal Genetics, Breeding and Reproduction, College of Animal Science, Huazhong Agricultural University, Wuhan 430070, China. shzhao@mail.hzau.edu.cn.
authors.forename: Shuhong
authors.initials: S
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Agriculture Animal Genetics, Breeding and Reproduction, College of Animal Science, Huazhong Agricultural University, Wuhan 430070, China. yzxionghzau@gmail.com.
authors.forename: Yuanzhu
authors.initials: Y
authors.lastname: Xiong
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D050156:Adipogenesis; D052242:Adiponectin / Q000235:genetics / Q000378:metabolism; D052436:Adipose Tissue, White / Q000166:cytology* / Q000378:metabolism; D000818:Animals; D023081:CCAAT-Binding Factor / Q000235:genetics / Q000378:metabolism; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D002478:Cells, Cultured; D050556:Fatty Acid-Binding Proteins / Q000235:genetics / Q000378:metabolism; D005346:Fibroblast Growth Factors / Q000235:genetics / Q000378:metabolism*; D051275:Glucose Transporter Type 4 / Q000235:genetics / Q000378:metabolism; D056466:Histone Demethylases / Q000235:genetics / Q000378:metabolism; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism; D000070778:Perilipin-1; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism; D013552:Swine
chemical_list: D052242:Adiponectin; D023081:CCAAT-Binding Factor; D002352:Carrier Proteins; D050556:Fatty Acid-Binding Proteins; D051275:Glucose Transporter Type 4; D047495:PPAR gamma; D000070778:Perilipin-1; D010750:Phosphoproteins; C414620:fibroblast growth factor 21; D005346:Fibroblast Growth Factors; D056466:Histone Demethylases
keywords: CEBPB; FGF21; IMT; adipogenesis; pig