title: Dicer interacts with SIRT7 and regulates H3K18 deacetylation in response to DNA damaging agents.
abstract: Dicer participates in heterochromatin formation in fission yeast and plants. However, whether it has a similar role in mammals remains controversial. Here we showed that the human Dicer protein interacts with SIRT7, an NAD(+)-dependent H3K18Ac (acetylated lysine 18 of histone H3) deacetylase, and holds a proportion of SIRT7 in the cytoplasm. Dicer knockdown led to an increase of chromatin-associated SIRT7 and simultaneously a decrease of cytoplasmic SIRT7, while its overexpression induced SIRT7 reduction in the chromatin-associated fraction and increment in the cytoplasm. Furthermore, DNA damaging agents promoted Dicer expression, leading to decreased level of chromatin-associated SIRT7 and increased level of H3K18Ac, which can be alleviated by Dicer knockdown. Taken together with that H3K18Ac was exclusively associated with the chromatin, our findings suggest that Dicer induction by DNA damaging treatments prevents H3K18Ac deacetylation, probably by trapping more SIRT7 in the cytoplasm.
authors.affiliation: Institute of Translational Medicine, First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325015, Zhejiang, P.R. China Cancer Center, First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325015, Zhejiang, P.R. China.
authors.forename: Pei-Ying
authors.initials: PY
authors.lastname: Zhang
authors.affiliation: Institute of Genomic Medicine, Wenzhou Medical University, Wenzhou 325015, Zhejiang, P.R. China.
authors.forename: Guiling
authors.initials: G
authors.lastname: Li
authors.affiliation: Institute of Translational Medicine, First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325015, Zhejiang, P.R. China Cancer Center, First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325015, Zhejiang, P.R. China.
authors.forename: Zhu-Jun
authors.initials: ZJ
authors.lastname: Deng
authors.affiliation: Department of Infection and Liver Diseases, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325015, Zhejiang, P.R. China.
authors.forename: Li-Yuan
authors.initials: LY
authors.lastname: Liu
authors.affiliation: Institute of Translational Medicine, First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325015, Zhejiang, P.R. China Cancer Center, First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325015, Zhejiang, P.R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Chen
authors.affiliation: Research Institute of Surgery, Daping Hospital, Third Military Medical University, Chongqing 400042, P.R. China.
authors.forename: Jun-Zhou
authors.initials: JZ
authors.lastname: Tang
authors.affiliation: Department of Infection and Liver Diseases, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325015, Zhejiang, P.R. China.
authors.forename: Yu-Qun
authors.initials: YQ
authors.lastname: Wang
authors.affiliation: Department of Infection and Liver Diseases, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325015, Zhejiang, P.R. China.
authors.forename: Su-Ting
authors.initials: ST
authors.lastname: Cao
authors.affiliation: Institute of Translational Medicine, First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325015, Zhejiang, P.R. China Cancer Center, First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325015, Zhejiang, P.R. China.
authors.forename: Yu-Xiao
authors.initials: YX
authors.lastname: Fang
authors.affiliation: Institute of Translational Medicine, First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325015, Zhejiang, P.R. China.
authors.forename: Fuping
authors.initials: F
authors.lastname: Wen
authors.affiliation: Institute of Translational Medicine, First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325015, Zhejiang, P.R. China Department of Dermato-Venereology, First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325015, Zhejiang, P.R. China.
authors.forename: Yunsheng
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Institute of Translational Medicine, First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325015, Zhejiang, P.R. China Department of Pediatric Surgery, First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325015, Zhejiang, P.R. China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Infection and Liver Diseases, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325015, Zhejiang, P.R. China.
authors.forename: Ke-Qing
authors.initials: KQ
authors.lastname: Shi
authors.affiliation: Department of Radiation Oncology, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325015, Zhejiang, P.R. China.
authors.forename: Wen-Feng
authors.initials: WF
authors.lastname: Li
authors.affiliation: Department of Radiation Oncology, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325015, Zhejiang, P.R. China.
authors.forename: Congying
authors.initials: C
authors.lastname: Xie
authors.affiliation: Institute of Translational Medicine, First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325015, Zhejiang, P.R. China Cancer Center, First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325015, Zhejiang, P.R. China Institute of Genomic Medicine, Wenzhou Medical University, Wenzhou 325015, Zhejiang, P.R. China tangkaifu@hotmail.com tang_kaifu@aliyun.com.
authors.forename: Kai-Fu
authors.initials: KF
authors.lastname: Tang
mesh_terms: D002460:Cell Line; D002843:Chromatin / Q000378:metabolism; D002945:Cisplatin / Q000633:toxicity; D053487:DEAD-box RNA Helicases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D004249:DNA Damage; D004317:Doxorubicin / Q000633:toxicity; D057809:HEK293 Cells; D006657:Histones / Q000378:metabolism*; D006801:Humans; D011839:Radiation, Ionizing; D043244:Ribonuclease III / Q000037:antagonists & inhibitors / Q000378:metabolism*; D037761:Sirtuins / Q000378:metabolism*
chemical_list: D002843:Chromatin; D006657:Histones; C510661:SIRT7 protein, human; D004317:Doxorubicin; C408087:DICER1 protein, human; D043244:Ribonuclease III; D037761:Sirtuins; D053487:DEAD-box RNA Helicases; D002945:Cisplatin