title: Investigation on the effect of fluorescence quenching of bovine serum albumin by cefoxitin sodium using fluorescence spectroscopy and synchronous fluorescence spectroscopy.
abstract: The reaction mechanism of cefoxitin sodium with bovine serum albumin was investigated using fluorescence spectroscopy and synchronous fluorescence spectroscopy at different temperatures. The results showed that the change of binding constant of the synchronous fluorescence method with increasing temperature could be used to estimate the types of quenching mechanisms of drugs with protein and was consistent with one of fluorescence quenching method. In addition, the number of binding sites, type of interaction force, cooperativity between drug and protein and energy-transfer parameters of cefoxitin sodium and bovine serum albumin obtained from two methods using the same equation were consistent. Electrostatic force played a major role in the conjugation reaction between bovine serum albumin and cefoxitin sodium, and the type of quenching was static quenching. The primary binding site for cefoxitin sodium was sub-hydrophobic domain IIA, and the number of binding sites was 1. The value of Hill's coefficients (nH ) was approximately equal to 1, which suggested no cooperativity in the bovine serum albumin-cefoxitin sodium system. The donor-to-acceptor distance r < 7 nm indicated that static fluorescence quenching of bovine serum albumin by cefoxitin sodium was also a non-radiation energy-transfer process. The results indicated that synchronous fluorescence spectrometry could be used to study the reaction mechanism between drug and protein, and was a useful supplement to the conventional method. Copyright © 2015 John Wiley & Sons, Ltd.
authors.affiliation: Key Laboratory of Analytical Science and Technology of He bei Province, College of Chemistry & Environmental Science, Hebei University, Baoding, 071002, Hebei Province, People's Republic of China.
authors.forename: Gaixia
authors.initials: G
authors.lastname: Li
authors.affiliation: Key Laboratory of Analytical Science and Technology of He bei Province, College of Chemistry & Environmental Science, Hebei University, Baoding, 071002, Hebei Province, People's Republic of China.
authors.forename: Bao-Sheng
authors.initials: BS
authors.lastname: Liu
authors.affiliation: Key Laboratory of Analytical Science and Technology of He bei Province, College of Chemistry & Environmental Science, Hebei University, Baoding, 071002, Hebei Province, People's Republic of China.
authors.forename: Qiuju
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Analytical Science and Technology of He bei Province, College of Chemistry & Environmental Science, Hebei University, Baoding, 071002, Hebei Province, People's Republic of China.
authors.forename: Rong
authors.initials: R
authors.lastname: Han
mesh_terms: D000818:Animals; D002417:Cattle; D002440:Cefoxitin / Q000737:chemistry*; D005453:Fluorescence; D015394:Molecular Structure; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature
chemical_list: D012710:Serum Albumin, Bovine; D002440:Cefoxitin
keywords: bovine serum albumin; cefoxitin sodium; fluorescence spectroscopy; reaction mechanism; synchronous fluorescence spectroscopy