title: Supporting data for the characterization of PNA-DNA four-way junctions.
abstract: Holliday or DNA four-way junctions (4WJs) are cruciform/bent structures composed of four DNA duplexes. 4WJs are key intermediates in homologous genetic recombination and double-strand break repair. To investigate 4WJs in vitro, junctions are assembled using four asymmetric DNA strands. The presence of four asymmetric strands about the junction branch point eliminates branch migration, and effectively immobilizes the resulting 4WJ. The purpose of these experiments is to show that immobile 4WJs composed of DNA and peptide nucleic acids (PNAs) can be distinguished from contaminating labile nucleic acid structures. These data compare the electrophoretic mobility of hybrid PNA-DNA junctions vs. i) a classic immobile DNA 4WJ, J1 and ii) contaminating nucleic acid structures.
authors.affiliation: Department of Chemistry and Biochemistry, The University of Southern Mississippi, Hattiesburg, MS, USA.
authors.forename: Douglas
authors.initials: D
authors.lastname: Iverson
authors.affiliation: Department of Chemistry and Biochemistry, The University of Southern Mississippi, Hattiesburg, MS, USA.
authors.forename: Crystal
authors.initials: C
authors.lastname: Serrano
authors.affiliation: Department of Chemistry and Biochemistry, The University of Southern Mississippi, Hattiesburg, MS, USA.
authors.forename: Ann Marie
authors.initials: AM
authors.lastname: Brahan
authors.affiliation: Department of Chemistry and Biochemistry, The University of Southern Mississippi, Hattiesburg, MS, USA.
authors.forename: Arik
authors.initials: A
authors.lastname: Shams
authors.affiliation: Department of Chemistry, New York University, New York, NY, USA.
authors.forename: Filbert
authors.initials: F
authors.lastname: Totsingan
authors.affiliation: Department of Chemistry and Biochemistry, The University of Southern Mississippi, Hattiesburg, MS, USA.
authors.forename: Anthony J
authors.initials: AJ
authors.lastname: Bell