title: Beneficial metabolic effects of selected probiotics on diet-induced obesity and insulin resistance in mice are associated with improvement of dysbiotic gut microbiota.
abstract: Alterations in gut microbiota composition and diversity were suggested to play a role in the development of obesity, a chronic subclinical inflammatory condition. We here evaluated the impact of oral consumption of a monostrain or multi-strain probiotic preparation in high-fat diet-induced obese mice. We observed a strain-specific effect and reported dissociation between the capacity of probiotics to dampen adipose tissue inflammation and to limit body weight gain. A multi-strain mixture was able to improve adiposity, insulin resistance and dyslipidemia through adipose tissue immune cell-remodelling, mainly affecting macrophages. At the gut level, the mixture modified the uptake of fatty acids and restored the expression level of the short-chain fatty acid receptor GPR43. These beneficial effects were associated with changes in the microbiota composition, such as the restoration of the abundance of Akkermansia muciniphila and Rikenellaceae and the decrease of other taxa like Lactobacillaceae. Using an in vitro gut model, we further showed that the probiotic mixture favours the production of butyrate and propionate. Our findings provide crucial clues for the design and use of more efficient probiotic preparations in obesity management and may bring new insights into the mechanisms by which host-microbe interactions govern such protective effects.
authors.affiliation: Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019 - UMR 8204 - CIIL - Centre d'Infection et d'Immunité de Lille, F-59000, Lille, France.
authors.forename: Jeanne
authors.initials: J
authors.lastname: Alard
authors.affiliation: Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019 - UMR 8204 - CIIL - Centre d'Infection et d'Immunité de Lille, F-59000, Lille, France.
authors.forename: Véronique
authors.initials: V
authors.lastname: Lehrter
authors.affiliation: INRA, UMR1319 MICALIS, Interaction Firmicutes Environment Group, Domaine de Vilvert, Jouy-en-Josas, France.|AgroParisTech, UMR MICALIS, Domaine de Vilvert, Jouy-en-Josas, France.
authors.forename: Moez
authors.initials: M
authors.lastname: Rhimi
authors.affiliation: Microbial Ecology Laboratory, Conservatoire National des Arts et Métiers (CNAM), Paris, France.
authors.forename: Irène
authors.initials: I
authors.lastname: Mangin
authors.affiliation: Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019 - UMR 8204 - CIIL - Centre d'Infection et d'Immunité de Lille, F-59000, Lille, France.
authors.forename: Véronique
authors.initials: V
authors.lastname: Peucelle
authors.affiliation: INRA, UMR1319 MICALIS, Interaction Firmicutes Environment Group, Domaine de Vilvert, Jouy-en-Josas, France.|AgroParisTech, UMR MICALIS, Domaine de Vilvert, Jouy-en-Josas, France.
authors.forename: Anne-Laure
authors.initials: AL
authors.lastname: Abraham
authors.affiliation: INRA, MaIAGE, Domaine de Vilvert, Jouy-en-Josas, France.
authors.forename: Mahendra
authors.identifier: 0000-0003-2986-354X
authors.initials: M
authors.lastname: Mariadassou
authors.affiliation: INRA, UMR1319 MICALIS, Interaction Firmicutes Environment Group, Domaine de Vilvert, Jouy-en-Josas, France.|AgroParisTech, UMR MICALIS, Domaine de Vilvert, Jouy-en-Josas, France.
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Maguin
authors.affiliation: Intestinal Ecosystem, Probiotics, Antibiotics, Université Paris Descartes, Paris, France.
authors.forename: Anne-Judith
authors.initials: AJ
authors.lastname: Waligora-Dupriet
authors.affiliation: Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019 - UMR 8204 - CIIL - Centre d'Infection et d'Immunité de Lille, F-59000, Lille, France.
authors.forename: Bruno
authors.initials: B
authors.lastname: Pot
authors.affiliation: Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019 - UMR 8204 - CIIL - Centre d'Infection et d'Immunité de Lille, F-59000, Lille, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Wolowczuk
authors.affiliation: Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019 - UMR 8204 - CIIL - Centre d'Infection et d'Immunité de Lille, F-59000, Lille, France.
authors.forename: Corinne
authors.initials: C
authors.lastname: Grangette
mesh_terms: D000818:Animals; D059305:Diet, High-Fat / Q000009:adverse effects*; D000069196:Gastrointestinal Microbiome / Q000502:physiology*; D007333:Insulin Resistance; D008297:Male; D051379:Mice; D064307:Microbiota; D009765:Obesity; D019936:Probiotics / Q000627:therapeutic use*