title: Dual-primer self-generation SERS signal amplification assay for PDGF-BB using label-free aptamer.
abstract: Highly sensitive detection of proteins, especially those associated with cancers, is essential to biomedical research as well as clinical diagnosis. In this work, a simple and novel one-two-three signal amplification surface-enhanced Raman scattering (SERS) method for the detection of protein is fabricated by using label-free aptamer and dual-primer self-generation. Platelet-derived growth factor B-chain (PDGF-BB) is selected as the model protein. The one-two-three cascade DNA amplification means one target-aptamer binding event, two hairpin DNA switches and three DNA amplification reactions. This strategy possesses some remarkable features compared to conventional signal amplification methods: (i) A smart probe including a label-free aptamer is fabricated, for suitable hybridization without hindering the affinity of the aptamer toward its target. (ii) Using the unique structure switch of the aptamer and cooperator, a one-two-three working mode is developed to amplify the SERS signal. The amplification efficiency is enhanced. Given the unique and attractive characteristics, a simple and universal strategy is designed to accomplish ultrasensitive detection of proteins. The detection limit of PDGF-BB via SERS detection is 0.42 pM, with the linear range from 1.0×10(-12)M to 10(-8)M. It is potentially universal because the aptamer can be easily designed for biomolecules whose aptamers undergo similar conformational changes.
authors.affiliation: Key Laboratory of Sensor Analysis of Tumor Marker Ministry of Education, State Key Laboratory Base of Eco-chemical Engineering, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.
authors.forename: SuJuan
authors.initials: S
authors.lastname: Ye
authors.affiliation: Key Laboratory of Sensor Analysis of Tumor Marker Ministry of Education, State Key Laboratory Base of Eco-chemical Engineering, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.
authors.forename: XiaoMo
authors.initials: X
authors.lastname: Zhai
authors.affiliation: Key Laboratory of Sensor Analysis of Tumor Marker Ministry of Education, State Key Laboratory Base of Eco-chemical Engineering, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.
authors.forename: YanYing
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Key Laboratory of Sensor Analysis of Tumor Marker Ministry of Education, State Key Laboratory Base of Eco-chemical Engineering, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China. Electronic address: kuang1009@126.com.
authors.forename: ShaoPing
authors.initials: S
authors.lastname: Kuang
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D000077214:Becaplermin; D015374:Biosensing Techniques / Q000379:methods; D017931:DNA Primers / Q000737:chemistry; D006801:Humans; D057230:Limit of Detection; D021141:Nucleic Acid Amplification Techniques / Q000379:methods; D020574:Proto-Oncogene Proteins c-sis / Q000032:analysis / Q000097:blood*; D013059:Spectrum Analysis, Raman / Q000379:methods*
chemical_list: D052157:Aptamers, Nucleotide; D017931:DNA Primers; D020574:Proto-Oncogene Proteins c-sis; D000077214:Becaplermin
keywords: PDGF-BB; Signal amplification; Surface-enhanced Raman scattering