title: Characterization of Linum usitatissimum L. oil obtained from different extraction technique and in vitro antioxidant potential of supercritical fluid extract.
abstract: OBJECTIVE
  Present investigation was aimed to characterize the fixed oil of Linum usitatissimum L. using five different extraction methods: Supercritical fluid extraction (SFE), ultrasound-assistance, soxhlet extraction, solvent extraction, and three phase partitioning method.
  
  
  METHODS
  The SFE conditions (temperature, pressure, and volume of CO2) were optimized prior for better yield. The extracted oils were analyzed and compared for their physiochemical parameters, high performance thin layer chromatography (HPTLC), gas chromatography-mass spectrometry (GC-MS), and Fourier-transformed infrared spectroscopy (FT-IR) fingerprinting. Antioxidant activity was also determined using 1,1-diphenyl-2-picrylhydrazyl and superoxide scavenging method.
  
  
  RESULTS
  The main fatty acids were α-linolenic acid, linoleic acid, palmitic acid, and stearic acid as obtained by GC-MS. HPTLC analysis revealed the presence of similar major components in chromatograms. Similarly, the pattern of peaks, as obtained in FT-IR and GC-MS spectra of same oils by different extraction methods, were superimposable.
  
  
  CONCLUSIONS
  Analysis reported that the fixed oil of L. usitatissimum L. is a good source of n-3 fatty acid with the significant antioxidant activity of oil obtained from SFE extraction method.
authors.affiliation: Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Jamia Hamdard University, New Delhi, India.
authors.forename: Rishika
authors.initials: R
authors.lastname: Chauhan
authors.affiliation: Department of Pharmacy, Bansthali Vidyapith, Bansthali University, Tonk, Rajasthan, India.
authors.forename: Karishma
authors.initials: K
authors.lastname: Chester
authors.affiliation: Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Jamia Hamdard University, New Delhi, India.
authors.forename: Yasmeen
authors.initials: Y
authors.lastname: Khan
authors.affiliation: Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Jamia Hamdard University, New Delhi, India.
authors.forename: Ennus Tajuddin
authors.initials: ET
authors.lastname: Tamboli
authors.affiliation: Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Jamia Hamdard University, New Delhi, India.
authors.forename: Sayeed
authors.initials: S
authors.lastname: Ahmad
keywords: Fixed oil; Fourier-transformed infrared; and supercritical fluid extraction; gas chromatography-mass spectrometry; high performance thin layer chromatography