title: Immunohistochemical detection of a potential molecular therapeutic target for canine hemangiosarcoma.
abstract: Canine hemangiosarcoma (HSA) is a progressive malignant neoplasm of dogs for which there is currently no effective treatment. A recent study suggested that receptor tyrosine kinases (RTKs), the PI3K/Akt/m-TOR and MAPK pathways are all activated in canine and human HSA. The aim of the present study was to investigate the overexpression of these proteins by immunohistochemistry in canine splenic HSA to identify potential molecular therapeutic targets. A total of 10 splenic HSAs and two normal splenic samples surgically resected from dogs were sectioned and stained with hematoxylin and eosin for histological diagnosis or analyzed using immunohistochemistry. The expression of RTKs, c-kit, VEGFR-2 and PDGFR-2, as well as PI3K/Akt/m-TOR and MEK was higher in canine splenic HSAs compared to normal spleens. These proteins may therefore be potential therapeutic targets in canine splenic HSA.
authors.affiliation: Laboratory of Advanced Veterinary Medicine, Department of Veterinary Clinical Sciences, Hokkaido University, Hokkaido 060-0818, Japan.
authors.forename: Mami
authors.initials: M
authors.lastname: Adachi
authors.forename: Yuki
authors.initials: Y
authors.lastname: Hoshino
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Izumi
authors.forename: Satoshi
authors.initials: S
authors.lastname: Takagi
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use*; D004283:Dog Diseases / Q000188:drug therapy* / Q000378:metabolism; D004285:Dogs; D016503:Drug Delivery Systems; D005260:Female; D006394:Hemangiosarcoma / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology / Q000662:veterinary*; D007150:Immunohistochemistry / Q000662:veterinary; D020935:MAP Kinase Signaling System / Q000187:drug effects; D008297:Male; D020794:Receptor Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism; D013160:Splenic Neoplasms / Q000188:drug therapy / Q000378:metabolism / Q000662:veterinary*; D058570:TOR Serine-Threonine Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D020794:Receptor Protein-Tyrosine Kinases; D058570:TOR Serine-Threonine Kinases