title: Whole-Mount Immunohistochemistry for Anti-F59 in Zebrafish Embryos (1-5 Days Post Fertilization (dpf)).
abstract: Immunohistochemistry (IHC) is a powerful method to determine localization of tissue components by the interaction of target antigens with labeled antibodies. Here we describe an IHC protocol for localizing the myosin heavy chain of zebrafish embryos at 1-2 and 3-5 days post fertilization (dpf).
authors.affiliation: Smith Cardiovascular Research Institute, University of California, San Francisco, USA.
authors.forename: Canan
authors.initials: C
authors.lastname: Doganli
authors.affiliation: Sanford-Burnham Medical Research Institute, La Jolla, USA.
authors.forename: Lucas
authors.initials: L
authors.lastname: Bukata
authors.affiliation: Department of Biomedicine and Centre for Membrane Pumps in Cells and Disease - PUMPKIN, Danish National Research Foundation, Aarhus University, Aarhus C, Denmark. kly@biomed.au.dk.|Department of Molecular Biology and Genetics, Aarhus University, Aarhus C, Denmark. kly@biomed.au.dk.|Aarhus Institute of Advanced Studies (AIAS), Aarhus University, Aarhus C, Denmark. kly@biomed.au.dk.
authors.forename: Karin
authors.initials: K
authors.lastname: Lykke-Hartmann
mesh_terms: D000818:Animals; D004625:Embryo, Nonmammalian / Q000378:metabolism* / Q000502:physiology; D005306:Fertilization; D055785:Gene Knockdown Techniques; D007150:Immunohistochemistry / Q000379:methods*; D007527:Isoenzymes / Q000172:deficiency / Q000235:genetics; D009154:Mutation; D018995:Myosin Heavy Chains / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000172:deficiency / Q000235:genetics; D013194:Staining and Labeling; D013997:Time Factors; D015027:Zebrafish / Q000196:embryology*
chemical_list: D007527:Isoenzymes; D018995:Myosin Heavy Chains; D000254:Sodium-Potassium-Exchanging ATPase
keywords: F59; Immunohistochemistry; Myosin heavy chain; Zebrafish