title: Multiplex-PCR for the detection of viruses in the CSF of infants and young children.
abstract: We aimed to establish the utility of the Fast-track diagnostics Viral meningitis multiplex PCR kit for the diagnosis of central nervous system infection in infants. The multiplex assay had reduced sensitivity for the detection of enterovirus, the predominant pathogen in young infants, when compared to our in-house singleplex PCR. In our infant population, multiple singleplex PCR assays perform better than a multiplex assay for the detection of CSF viruses. J. Med. Virol. 89:559-561, 2017. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Department of Paediatrics, University of Otago, Christchurch, Christchurch, New Zealand.
authors.forename: Tony
authors.initials: T
authors.lastname: Walls
authors.affiliation: Department of Paediatrics, University of Otago, Christchurch, Christchurch, New Zealand.|Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand.
authors.forename: Alice
authors.initials: A
authors.lastname: McSweeney
authors.affiliation: Department of Microbiology, Canterbury Health Laboratories, Christchurch, New Zealand.
authors.forename: Trevor
authors.initials: T
authors.lastname: Anderson
authors.affiliation: Department of Microbiology, Canterbury Health Laboratories, Christchurch, New Zealand.
authors.forename: Lance C
authors.initials: LC
authors.lastname: Jennings
mesh_terms: D002555:Cerebrospinal Fluid / Q000821:virology*; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008587:Meningitis, Viral / Q000175:diagnosis*; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D014780:Viruses / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*
keywords: enterovirus; infants; multiplex PCR; viral meningitis