title: CD90 and CD105 expression in the mouse ovary and testis at different stages of postnatal development.
abstract: CD90 (i.e., THY1) and CD105 (i.e., endoglin) are glycoproteins known as mesenchymal stem cell markers that are expressed in various cell types including male and female gonadal cells. We aimed to determine ovarian and testicular expression of CD90 and CD105 in various cell types during postnatal development in mice. The present study was carried out on male (C57BL/6) and female (Balb/C) mice during critical stages of gonadal development. Immunohistochemical localization of CD90 and CD105 was determined in the ovaries obtained at postnatal days (PND) -1, -7, -21 and -60 and in the testes obtained at PND6, -8, -16, -20, -29, -32 and -88. The relative expression of CD90 and CD105 was evaluated by ImageJ software and data were analyzed by analysis of variance. The relative expression of CD90 and CD105 varied during postnatal development and increased significantly in the adult ovary (PND60) and testis (PND88) compared to the early postnatal gonads. In the ovaries, the expression of CD90 was significantly higher in somatic cells in comparison to germ cell compartments. In the testis, CD90 expression was greater in germ cells and Sertoli cells compared to other cell types. Expression of CD105 was higher in germ cells than somatic cells of both the ovary and testis. In addition to different expression of CD90 and CD105 during various developmental stages, also their altered expression in particular cell types suggests specific roles of these glycoproteins in physiological processes of mouse gonads.
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, Akdeniz University, 07070, Campus, Antalya, Turkey.
authors.forename: Filiz
authors.initials: F
authors.lastname: Tepekoy
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, Akdeniz University, 07070, Campus, Antalya, Turkey.
authors.forename: Saffet
authors.initials: S
authors.lastname: Ozturk
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, Akdeniz University, 07070, Campus, Antalya, Turkey.
authors.forename: Berna
authors.initials: B
authors.lastname: Sozen
authors.affiliation: Faculty of Medicine, Akdeniz University, 07070, Campus, Antalya, Turkey.
authors.forename: Recep S
authors.initials: RS
authors.lastname: Ozay
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, Akdeniz University, 07070, Campus, Antalya, Turkey.
authors.forename: Gokhan
authors.initials: G
authors.lastname: Akkoyunlu
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, Akdeniz University, 07070, Campus, Antalya, Turkey. Electronic address: ndemir@akdeniz.edu.tr.
authors.forename: Necdet
authors.initials: N
authors.lastname: Demir
mesh_terms: D000375:Aging; D000818:Animals; D000071063:Endoglin; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D007150:Immunohistochemistry; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D010053:Ovary / Q000254:growth & development / Q000378:metabolism*; D013737:Testis / Q000254:growth & development / Q000378:metabolism*; D018800:Thy-1 Antigens / Q000235:genetics / Q000378:metabolism*
chemical_list: D000071063:Endoglin; C502004:Eng protein, mouse; D047908:Intracellular Signaling Peptides and Proteins; D018800:Thy-1 Antigens
keywords: CD105; CD90; Ovary; Testis