title: Effect of low-dose malathion on the gonadal development of adult rare minnow Gobiocypris rarus.
abstract: Malathion is an organophosphorus pesticide that extensively used in agriculture and veterinary practices. To investigate the effects of low dose malathion on rare minnow Gobiocypris rarus gonadal development, we exposed adult rare minnow to environmentally relevant concentration malathion (2 and 20μg/L) for 21 days. Gonadal histology, sex hormone levels and mRNA expressions of steroidogenic genes were investigated. Malathion at both 2 and 20μg/L significantly up-regulated rare minnow testicular weight and promoted the progression of spermatogenesis. Neither ovarian weight nor process of ovary development was markedly changed. In testis, 2μg/L malathion significantly down-regulated testosterone and 11-ketotestosterone levels, and up-regulated mRNA expression of steroidogenic genes. In ovaries, 2 and 20μg/L malathion significantly inhibited estradiol17β levels and induced testosterone levels, both in concentration dependent manners; mRNA expressions of almost all the detected ovarian steroidogenic genes were up-regulated. The present result suggested that malathion even at low dose could pose a potential threat to adult rare minnow gonadal development.
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China.
authors.forename: Lang
authors.initials: L
authors.lastname: Wu
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China.
authors.forename: Guo
authors.initials: G
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China.
authors.forename: Yongjing
authors.initials: Y
authors.lastname: Guan
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China. Electronic address: zzwang@nwsuaf.edu.cn.
authors.forename: Zaizhao
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000818:Animals; D003530:Cyprinidae / Q000196:embryology*; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation; D004781:Environmental Exposure / Q000009:adverse effects*; D004958:Estradiol / Q000378:metabolism; D005260:Female; D012739:Gonadal Steroid Hormones / Q000378:metabolism; D006066:Gonads / Q000187:drug effects* / Q000378:metabolism; D008294:Malathion / Q000008:administration & dosage / Q000633:toxicity*; D008297:Male; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013739:Testosterone / Q000031:analogs & derivatives / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D012739:Gonadal Steroid Hormones; D012333:RNA, Messenger; D013739:Testosterone; D004958:Estradiol; C003600:11-ketotestosterone; D008294:Malathion
keywords: Gonadal development; Malathion; Rare minnow Gobiocypris rarus; Sex hormone; Steroidogenic gene