title: Analysis of Indole Alkaloids from Rhazya stricta Hairy Roots by Ultra-Performance Liquid Chromatography-Mass Spectrometry.
abstract: Rhazya stricta Decne. (Apocynaceae) contains a large number of terpenoid indole alkaloids (TIAs). This study focused on the composition of alkaloids obtained from transformed hairy root cultures of R. stricta employing ultra-performance liquid chromatography-mass spectrometry (UPLC-MS). In the UPLC-MS analyses, a total of 20 TIAs were identified from crude extracts. Eburenine and vincanine were the main alkaloids followed by polar glucoalkaloids, strictosidine lactam and strictosidine. Secodine-type alkaloids, tetrahydrosecodinol, tetrahydro- and dihydrosecodine were detected too. The occurrence of tetrahydrosecodinol was confirmed for the first time for R. stricta. Furthermore, two isomers of yohimbine, serpentine and vallesiachotamine were identified. The study shows that a characteristic pattern of biosynthetically related TIAs can be monitored in Rhazya hairy root crude extract by this chromatographic method.
authors.affiliation: VTT Technical Research Centre of Finland Ltd., P.O. Box 1000, FI-02044 VTT, Finland. amirakhgari1357@gmail.com.|Division of Pharmaceutical Biosciences, Faculty of Pharmacy, P.O. Box 56, FI-00014 University of Helsinki, Finland. amirakhgari1357@gmail.com.
authors.forename: Amir
authors.initials: A
authors.lastname: Akhgari
authors.affiliation: Division of Pharmaceutical Biosciences, Faculty of Pharmacy, P.O. Box 56, FI-00014 University of Helsinki, Finland. into.laakso@helsinki.fi.
authors.forename: Into
authors.initials: I
authors.lastname: Laakso
authors.affiliation: VTT Technical Research Centre of Finland Ltd., P.O. Box 1000, FI-02044 VTT, Finland. tuulikki.seppanen-laakso@vtt.fi.
authors.forename: Tuulikki
authors.initials: T
authors.lastname: Seppänen-Laakso
authors.affiliation: Division of Pharmaceutical Biosciences, Faculty of Pharmacy, P.O. Box 56, FI-00014 University of Helsinki, Finland. teijo.yrjonen@helsinki.fi.
authors.forename: Teijo
authors.initials: T
authors.lastname: Yrjönen
authors.affiliation: Division of Pharmaceutical Biosciences, Faculty of Pharmacy, P.O. Box 56, FI-00014 University of Helsinki, Finland. heikki.vuorela@helsinki.fi.
authors.forename: Heikki
authors.initials: H
authors.lastname: Vuorela
authors.affiliation: VTT Technical Research Centre of Finland Ltd., P.O. Box 1000, FI-02044 VTT, Finland. kirsi-marja.oksman@vtt.fi.
authors.forename: Kirsi-Marja
authors.initials: KM
authors.lastname: Oksman-Caldentey
authors.affiliation: VTT Technical Research Centre of Finland Ltd., P.O. Box 1000, FI-02044 VTT, Finland. heiko.rischer@vtt.fi.
authors.forename: Heiko
authors.initials: H
authors.lastname: Rischer
mesh_terms: D000470:Alkaloids / Q000737:chemistry / Q000302:isolation & purification*; D029062:Apocynaceae / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D056148:Chromatography, Reverse-Phase; D007211:Indoles / Q000737:chemistry / Q000302:isolation & purification*; D018517:Plant Roots / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D000470:Alkaloids; D007211:Indoles
keywords: Rhazya stricta; terpenoid indole alkaloids; ultra-performance liquid chromatography-mass spectrometry