title: Polycystic ovary syndrome is associated with increased osteopontin levels.
abstract: OBJECTIVE
  Osteopontin (OPN) is a multi-functional secreted glycoprotein that plays a crucial role in glucose metabolism and inflammatory process. Growing evidence suggests that there is a link between OPN and ovarian function. However, no such link has yet been found for OPN in polycystic ovary syndrome (PCOS). Our aim was to ascertain whether circulating OPN levels are altered in women with PCOS and to determine whether OPN levels differ between the follicular phase and mid-cycle of the menstrual cycle in eumenorrheic women.
  
  
  METHODS
  In total, 150 women with PCOS and 150 age- and BMI-matched controls without PCOS were recruited for this prospective observational study. OPN levels were measured using ELISA. Metabolic parameters were also determined.
  
  
  RESULTS
  Circulating OPN levels were significantly elevated in PCOS women compared with controls (69.12±31.59  ng/ml vs 42.66±21.28  ng/ml; P<0.001). OPN levels were significantly higher at mid-cycle than in the follicular phase in eumenorrheic women. OPN was positively correlated with BMI, homeostasis model assessment of insulin resistance (HOMA-IR), free testosterone, and high sensitivity C-reactive protein (hs-CRP). Multivariate logistic regression analyses revealed that the odds ratio (OR) for PCOS was 3.64 for patients in the highest quartile of OPN compared with those in the lowest quartile (OR=3.64; 95% CI=2.42-5.57; P=0.011). Our findings indicate that BMI, HOMA-IR, hs-CRP, and free testosterone are independent factors influencing serum OPN levels and that OPN is an independent predictor for HOMA-IR.
  
  
  CONCLUSIONS
  PCOS is associated with increased OPN levels.
authors.forename: Ali
authors.initials: A
authors.lastname: Saklamaz
authors.affiliation: Division of Endocrinology and MetabolismDepartment of Internal Medicine, Sifa University School of Medicine, 35410 Izmir, TurkeyPCOS Research GroupDivision of Endocrinology and Metabolism, Department of Internal Medicine, Izmir Bozyaka Training and Research Hospital, 35170 Bozyaka, Izmir, TurkeyPCOS Research GroupDepartment of Obstetrics and Gynecology, Manisa Merkezefendi State Hospital, 45020 Merkezefendi, Manisa, TurkeyDepartment of Biochemistry and Clinical BiochemistryDokuz Eylul University Faculty of Medicine, 35340 Inciralti, Izmir, TurkeyDepartment of Obstetrics and GynecologySifa University School of Medicine, 35410 Izmir, TurkeyDivision of Endocrinology and MetabolismDepartment of Internal Medicine, Ege University Medical School, 35100 Bornova, Izmir, Turkey drmehmetcalan@gmail.com.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Calan
authors.affiliation: Division of Endocrinology and MetabolismDepartment of Internal Medicine, Sifa University School of Medicine, 35410 Izmir, TurkeyPCOS Research GroupDivision of Endocrinology and Metabolism, Department of Internal Medicine, Izmir Bozyaka Training and Research Hospital, 35170 Bozyaka, Izmir, TurkeyPCOS Research GroupDepartment of Obstetrics and Gynecology, Manisa Merkezefendi State Hospital, 45020 Merkezefendi, Manisa, TurkeyDepartment of Biochemistry and Clinical BiochemistryDokuz Eylul University Faculty of Medicine, 35340 Inciralti, Izmir, TurkeyDepartment of Obstetrics and GynecologySifa University School of Medicine, 35410 Izmir, TurkeyDivision of Endocrinology and MetabolismDepartment of Internal Medicine, Ege University Medical School, 35100 Bornova, Izmir, Turkey.
authors.forename: Ozgur
authors.initials: O
authors.lastname: Yilmaz
authors.affiliation: Division of Endocrinology and MetabolismDepartment of Internal Medicine, Sifa University School of Medicine, 35410 Izmir, TurkeyPCOS Research GroupDivision of Endocrinology and Metabolism, Department of Internal Medicine, Izmir Bozyaka Training and Research Hospital, 35170 Bozyaka, Izmir, TurkeyPCOS Research GroupDepartment of Obstetrics and Gynecology, Manisa Merkezefendi State Hospital, 45020 Merkezefendi, Manisa, TurkeyDepartment of Biochemistry and Clinical BiochemistryDokuz Eylul University Faculty of Medicine, 35340 Inciralti, Izmir, TurkeyDepartment of Obstetrics and GynecologySifa University School of Medicine, 35410 Izmir, TurkeyDivision of Endocrinology and MetabolismDepartment of Internal Medicine, Ege University Medical School, 35100 Bornova, Izmir, Turkey.
authors.forename: Tuncay
authors.initials: T
authors.lastname: Kume
authors.affiliation: Division of Endocrinology and MetabolismDepartment of Internal Medicine, Sifa University School of Medicine, 35410 Izmir, TurkeyPCOS Research GroupDivision of Endocrinology and Metabolism, Department of Internal Medicine, Izmir Bozyaka Training and Research Hospital, 35170 Bozyaka, Izmir, TurkeyPCOS Research GroupDepartment of Obstetrics and Gynecology, Manisa Merkezefendi State Hospital, 45020 Merkezefendi, Manisa, TurkeyDepartment of Biochemistry and Clinical BiochemistryDokuz Eylul University Faculty of Medicine, 35340 Inciralti, Izmir, TurkeyDepartment of Obstetrics and GynecologySifa University School of Medicine, 35410 Izmir, TurkeyDivision of Endocrinology and MetabolismDepartment of Internal Medicine, Ege University Medical School, 35100 Bornova, Izmir, Turkey.
authors.forename: Muzaffer
authors.initials: M
authors.lastname: Temur
authors.affiliation: Division of Endocrinology and MetabolismDepartment of Internal Medicine, Sifa University School of Medicine, 35410 Izmir, TurkeyPCOS Research GroupDivision of Endocrinology and Metabolism, Department of Internal Medicine, Izmir Bozyaka Training and Research Hospital, 35170 Bozyaka, Izmir, TurkeyPCOS Research GroupDepartment of Obstetrics and Gynecology, Manisa Merkezefendi State Hospital, 45020 Merkezefendi, Manisa, TurkeyDepartment of Biochemistry and Clinical BiochemistryDokuz Eylul University Faculty of Medicine, 35340 Inciralti, Izmir, TurkeyDepartment of Obstetrics and GynecologySifa University School of Medicine, 35410 Izmir, TurkeyDivision of Endocrinology and MetabolismDepartment of Internal Medicine, Ege University Medical School, 35100 Bornova, Izmir, Turkey.
authors.forename: Nurdan
authors.initials: N
authors.lastname: Yildiz
authors.affiliation: Division of Endocrinology and MetabolismDepartment of Internal Medicine, Sifa University School of Medicine, 35410 Izmir, TurkeyPCOS Research GroupDivision of Endocrinology and Metabolism, Department of Internal Medicine, Izmir Bozyaka Training and Research Hospital, 35170 Bozyaka, Izmir, TurkeyPCOS Research GroupDepartment of Obstetrics and Gynecology, Manisa Merkezefendi State Hospital, 45020 Merkezefendi, Manisa, TurkeyDepartment of Biochemistry and Clinical BiochemistryDokuz Eylul University Faculty of Medicine, 35340 Inciralti, Izmir, TurkeyDepartment of Obstetrics and GynecologySifa University School of Medicine, 35410 Izmir, TurkeyDivision of Endocrinology and MetabolismDepartment of Internal Medicine, Ege University Medical School, 35100 Bornova, Izmir, Turkey.
authors.forename: Esin
authors.initials: E
authors.lastname: Kasap
authors.affiliation: Division of Endocrinology and MetabolismDepartment of Internal Medicine, Sifa University School of Medicine, 35410 Izmir, TurkeyPCOS Research GroupDivision of Endocrinology and Metabolism, Department of Internal Medicine, Izmir Bozyaka Training and Research Hospital, 35170 Bozyaka, Izmir, TurkeyPCOS Research GroupDepartment of Obstetrics and Gynecology, Manisa Merkezefendi State Hospital, 45020 Merkezefendi, Manisa, TurkeyDepartment of Biochemistry and Clinical BiochemistryDokuz Eylul University Faculty of Medicine, 35340 Inciralti, Izmir, TurkeyDepartment of Obstetrics and GynecologySifa University School of Medicine, 35410 Izmir, TurkeyDivision of Endocrinology and MetabolismDepartment of Internal Medicine, Ege University Medical School, 35100 Bornova, Izmir, Turkey.
authors.forename: Mine
authors.initials: M
authors.lastname: Genc
authors.affiliation: Division of Endocrinology and MetabolismDepartment of Internal Medicine, Sifa University School of Medicine, 35410 Izmir, TurkeyPCOS Research GroupDivision of Endocrinology and Metabolism, Department of Internal Medicine, Izmir Bozyaka Training and Research Hospital, 35170 Bozyaka, Izmir, TurkeyPCOS Research GroupDepartment of Obstetrics and Gynecology, Manisa Merkezefendi State Hospital, 45020 Merkezefendi, Manisa, TurkeyDepartment of Biochemistry and Clinical BiochemistryDokuz Eylul University Faculty of Medicine, 35340 Inciralti, Izmir, TurkeyDepartment of Obstetrics and GynecologySifa University School of Medicine, 35410 Izmir, TurkeyDivision of Endocrinology and MetabolismDepartment of Internal Medicine, Ege University Medical School, 35100 Bornova, Izmir, Turkey.
authors.forename: Banu
authors.initials: B
authors.lastname: Sarer Yurekli
authors.affiliation: Division of Endocrinology and MetabolismDepartment of Internal Medicine, Sifa University School of Medicine, 35410 Izmir, TurkeyPCOS Research GroupDivision of Endocrinology and Metabolism, Department of Internal Medicine, Izmir Bozyaka Training and Research Hospital, 35170 Bozyaka, Izmir, TurkeyPCOS Research GroupDepartment of Obstetrics and Gynecology, Manisa Merkezefendi State Hospital, 45020 Merkezefendi, Manisa, TurkeyDepartment of Biochemistry and Clinical BiochemistryDokuz Eylul University Faculty of Medicine, 35340 Inciralti, Izmir, TurkeyDepartment of Obstetrics and GynecologySifa University School of Medicine, 35410 Izmir, TurkeyDivision of Endocrinology and MetabolismDepartment of Internal Medicine, Ege University Medical School, 35100 Bornova, Izmir, Turkey.
authors.forename: Gokcen
authors.initials: G
authors.lastname: Unal Kocabas
mesh_terms: D000328:Adult; D015992:Body Mass Index; D002097:C-Reactive Protein / Q000032:analysis; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D007333:Insulin Resistance; D008055:Lipids / Q000097:blood; D008597:Menstrual Cycle / Q000097:blood; D053495:Osteopontin / Q000097:blood*; D011085:Polycystic Ovary Syndrome / Q000097:blood* / Q000453:epidemiology; D013739:Testosterone / Q000097:blood; D055815:Young Adult
chemical_list: D008055:Lipids; D053495:Osteopontin; D013739:Testosterone; D002097:C-Reactive Protein