title: A Pan-HIV Strategy for Complete Genome Sequencing.
abstract: Molecular surveillance is essential to monitor HIV diversity and track emerging strains. We have developed a universal library preparation method (HIV-SMART [i.e.,switchingmechanismat 5' end ofRNAtranscript]) for next-generation sequencing that harnesses the specificity of HIV-directed priming to enable full genome characterization of all HIV-1 groups (M, N, O, and P) and HIV-2. Broad application of the HIV-SMART approach was demonstrated using a panel of diverse cell-cultured virus isolates. HIV-1 non-subtype B-infected clinical specimens from Cameroon were then used to optimize the protocol to sequence directly from plasma. When multiplexing 8 or more libraries per MiSeq run, full genome coverage at a median ∼2,000× depth was routinely obtained for either sample type. The method reproducibly generated the same consensus sequence, consistently identified viral sequence heterogeneity present in specimens, and at viral loads of ≤4.5 log copies/ml yielded sufficient coverage to permit strain classification. HIV-SMART provides an unparalleled opportunity to identify diverse HIV strains in patient specimens and to determine phylogenetic classification based on the entire viral genome. Easily adapted to sequence any RNA virus, this technology illustrates the utility of next-generation sequencing (NGS) for viral characterization and surveillance.
authors.affiliation: Infectious Diseases Research, Abbott Diagnostics, Abbott Park, Illinois, USA michael.berg@abbott.com.
authors.forename: Michael G
authors.initials: MG
authors.lastname: Berg
authors.affiliation: Infectious Diseases Research, Abbott Diagnostics, Abbott Park, Illinois, USA.
authors.forename: Julie
authors.initials: J
authors.lastname: Yamaguchi
authors.affiliation: Virology Unit, National Reference for HIV, Centre Hospitalier Universitaire de Rouen, Rouen, France.
authors.forename: Elodie
authors.initials: E
authors.lastname: Alessandri-Gradt
authors.affiliation: Infectious Diseases Research, Abbott Diagnostics, Abbott Park, Illinois, USA.
authors.forename: Robert W
authors.initials: RW
authors.lastname: Tell
authors.affiliation: Virology Unit, National Reference for HIV, Centre Hospitalier Universitaire de Rouen, Rouen, France.
authors.forename: Jean-Christophe
authors.identifier: http://orcid.org/0000-0001-6417-4083
authors.initials: JC
authors.lastname: Plantier
authors.affiliation: Infectious Diseases Research, Abbott Diagnostics, Abbott Park, Illinois, USA.
authors.forename: Catherine A
authors.initials: CA
authors.lastname: Brennan
mesh_terms: D002163:Cameroon; D016679:Genome, Viral; D005838:Genotype; D006678:HIV / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D015658:HIV Infections / Q000821:virology*; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006801:Humans; D010802:Phylogeny; D010949:Plasma / Q000821:virology*; D017422:Sequence Analysis, DNA / Q000379:methods*