title: Dual-Mode Ultrasensitive Quantification of MicroRNA in Living Cells by Chiroplasmonic Nanopyramids Self-Assembled from Gold and Upconversion Nanoparticles.
abstract: Chiral self-assembled nanomaterials with biological applications have attracted great interest. In this study, DNA-driven gold-upconversion nanoparticle (Au-UCNP) pyramids were fabricated to detect intracellular microRNA (miRNA) in real time. The Au-UCNP pyramids are doubly optically active, displaying strong plasmonic circular dichroism (CD) at 521 nm and significant luminescence in 500-600 nm, and therefore can be monitored by both of them. CD will decrease while the luminescence intensity increases in the presence of miRNA. The experimental results show that the CD intensity had an outstanding linear range from 0.073 to 43.65 fmol/10 μg(RNA) and a limit of detection (LOD) of 0.03 fmol/10 μg(RNA), whereas the luminescence intensity ranged from 0.16 to 43.65 fmol/10 μg(RNA) with a LOD of 0.12 fmol/10 μg(RNA). These data indicate that the CD signal is much more sensitive to the concentration of miRNA than the luminescent signal, which is attributed to the strong CD intensity arising from the spin angular momentum of the photon interaction with chiral nanostructures and the plasmonic enhancement of the intrinsic chirality of DNA molecules in the pyramids. This approach opens up a new avenue to the ultrasensitive detection and quantification of miRNA in living cells.
authors.affiliation: State Key Lab of Food Science and Technology, International Joint Research Laboratory for Biointerface and Biodetection, School of Food Science and Technology, Jiangnan University , Wuxi, Jiangsu 214122, People's Republic of China.
authors.forename: Si
authors.initials: S
authors.lastname: Li
authors.affiliation: State Key Lab of Food Science and Technology, International Joint Research Laboratory for Biointerface and Biodetection, School of Food Science and Technology, Jiangnan University , Wuxi, Jiangsu 214122, People's Republic of China.
authors.forename: Liguang
authors.initials: L
authors.lastname: Xu
authors.affiliation: State Key Lab of Food Science and Technology, International Joint Research Laboratory for Biointerface and Biodetection, School of Food Science and Technology, Jiangnan University , Wuxi, Jiangsu 214122, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Ma
authors.affiliation: State Key Lab of Food Science and Technology, International Joint Research Laboratory for Biointerface and Biodetection, School of Food Science and Technology, Jiangnan University , Wuxi, Jiangsu 214122, People's Republic of China.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Wu
authors.affiliation: State Key Lab of Food Science and Technology, International Joint Research Laboratory for Biointerface and Biodetection, School of Food Science and Technology, Jiangnan University , Wuxi, Jiangsu 214122, People's Republic of China.
authors.forename: Maozhong
authors.initials: M
authors.lastname: Sun
authors.affiliation: State Key Lab of Food Science and Technology, International Joint Research Laboratory for Biointerface and Biodetection, School of Food Science and Technology, Jiangnan University , Wuxi, Jiangsu 214122, People's Republic of China.
authors.forename: Hua
authors.initials: H
authors.lastname: Kuang
authors.affiliation: State Key Lab of Food Science and Technology, International Joint Research Laboratory for Biointerface and Biodetection, School of Food Science and Technology, Jiangnan University , Wuxi, Jiangsu 214122, People's Republic of China.
authors.forename: Libing
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Chemical Engineering, University of Michigan , Ann Arbor, Michigan 48109-2136, United States.
authors.forename: Nicholas A
authors.initials: NA
authors.lastname: Kotov
authors.affiliation: State Key Lab of Food Science and Technology, International Joint Research Laboratory for Biointerface and Biodetection, School of Food Science and Technology, Jiangnan University , Wuxi, Jiangsu 214122, People's Republic of China.
authors.forename: Chuanlai
authors.initials: C
authors.lastname: Xu
mesh_terms: D002942:Circular Dichroism; D006046:Gold / Q000737:chemistry*; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry*; D035683:MicroRNAs / Q000032:analysis*; D046529:Microscopy, Electron, Transmission; D013056:Spectrophotometry, Ultraviolet
chemical_list: D035683:MicroRNAs; D006046:Gold