title: Dexmedetomidine Pretreatment Attenuates Kidney Injury and Oxidative Stress during Orthotopic Autologous Liver Transplantation in Rats.
abstract: This paper aims to explore whether pretreatment with dexmedetomidine (Dex) has antioxidative and renal protective effects during orthotopic autologous liver transplantation (OALT) and its impact on nuclear factor erythroid 2-related factor 2 (Nrf2) activation. Sprague-Dawley rats were randomized into groups that include sham-operated (group S), model (group M), low dose Dex (group D1), high dose Dex (group D2), atipamezole (a nonspecific α2 receptor blocker) + high dose Dex (group B1), ARC239 (a specific α2B/c receptor blocker) + high dose Dex (group B2), and BRL-44408 (a specific α2A receptor blocker) + high dose Dex (group B3). Then histopathologic examination of the kidneys and measurement of renal function, the renal Nrf2 protein expression, and oxidants and antioxidants were performed 8 hours after OALT. We found that pretreatment with Dex activated Nrf2 in glomerular cells and upregulated antioxidants but reduced oxidants (all P < 0.01, group D2 versus group M). Atipamezole and BRL-44408, but not ARC239, reversed these protective effects. In conclusion, pretreatment with Dex activates Nrf2 through α2A receptor, increases the antioxidant levels, and attenuates renal injury during OALT.
authors.affiliation: Department of Anesthesiology, Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510630, China ; Department of Anesthesiology, Fujian Provincial Hospital, Fuzhou, Fujian 350001, China.
authors.forename: Xiaofang
authors.identifier: 0000-0002-7000-4328
authors.initials: X
authors.lastname: Yu
authors.affiliation: Department of Anesthesiology, Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510630, China.
authors.forename: Xinjin
authors.identifier: 0000-0001-8215-3235
authors.initials: X
authors.lastname: Chi
authors.affiliation: Department of Anesthesiology, Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510630, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Wu
authors.affiliation: Department of Pathology, Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510630, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Jin
authors.affiliation: Department of Anesthesiology, Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510630, China.
authors.forename: Hui
authors.identifier: 0000-0002-4906-8044
authors.initials: H
authors.lastname: Yao
authors.affiliation: Department of Anesthesiology, Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510630, China ; Department of Anesthesiology, First Affiliated Hospital, University of South China, Hengyang, Hunan 421001, China.
authors.forename: Yiheng
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Anesthesiology, The Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325027, China.
authors.forename: Zhengyuan
authors.identifier: 0000-0002-7002-5524
authors.initials: Z
authors.lastname: Xia
authors.affiliation: Department of Anesthesiology, Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510630, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Cai
mesh_terms: D000818:Animals; D064592:Autografts; D020927:Dexmedetomidine / Q000494:pharmacology*; D007668:Kidney / Q000293:injuries* / Q000378:metabolism / Q000473:pathology; D007674:Kidney Diseases / Q000209:etiology / Q000378:metabolism / Q000517:prevention & control*; D016031:Liver Transplantation; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D020927:Dexmedetomidine