title: Preparation and antibacterial properties of O-carboxymethyl chitosan/lincomycin hydrogels.
abstract: In this study, O-carboxymethyl chitosan (O-CMCS) was synthesized from chitosan and monochloroacetic acid. Then O-CMCS hydrogel was prepared by 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) in which the lincomycin was packaged. The Fourier transform infrared spectrum and scanning electron microscopy were adopted to characterize the structure and morphology of the product. The influences of dosage of EDC/NHS and concentration of O-CMCS on the swelling properties of the hydrogels were investigated. The hydrogels performed good swelling capacities and obvious pH-sensitive properties. The antibacterial activities of the hydrogels were tested against Gram-negative Escherichia coli (E. coli) and Gram-positive Staphylococcus aureus (S. aureus). Compared with pure O-CMCS hydrogels, the antibacterial activities of O-CMCS/lincomycin hydrogels were significantly improved with the increase in the concentration of lincomycin against E. coli and S. aureus. With the increase in dosage of crosslinking agent or concentration of O-CMCS, the antibacterial activities both decreased gradually against the two bacteria. O-CMCS/lincomycin hydrogel was expected to be used for antibacterial material in view of its significant antibacterial activities.
authors.affiliation: a School of Chemistry, Chemical Engineering and Life Sciences , Wuhan University of Technology , Wuhan , China.
authors.forename: Guanghua
authors.initials: G
authors.lastname: He
authors.affiliation: a School of Chemistry, Chemical Engineering and Life Sciences , Wuhan University of Technology , Wuhan , China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Chen
authors.affiliation: a School of Chemistry, Chemical Engineering and Life Sciences , Wuhan University of Technology , Wuhan , China.
authors.forename: Yihua
authors.initials: Y
authors.lastname: Yin
authors.affiliation: a School of Chemistry, Chemical Engineering and Life Sciences , Wuhan University of Technology , Wuhan , China.
authors.forename: Weiquan
authors.initials: W
authors.lastname: Cai
authors.affiliation: a School of Chemistry, Chemical Engineering and Life Sciences , Wuhan University of Technology , Wuhan , China.
authors.forename: Wanwan
authors.initials: W
authors.lastname: Ke
authors.affiliation: a School of Chemistry, Chemical Engineering and Life Sciences , Wuhan University of Technology , Wuhan , China.
authors.forename: Yahui
authors.initials: Y
authors.lastname: Kong
authors.affiliation: a School of Chemistry, Chemical Engineering and Life Sciences , Wuhan University of Technology , Wuhan , China.
authors.forename: Hua
authors.initials: H
authors.lastname: Zheng
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology*; D048271:Chitosan / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry; D004926:Escherichia coli / Q000187:drug effects; D020100:Hydrogels / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008034:Lincomycin / Q000737:chemistry* / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D020100:Hydrogels; C504164:O-carboxymethylchitosan; D048271:Chitosan; D008034:Lincomycin
keywords: O-carboxymethyl chitosan; antibacterial properties; hydrogel; lincomycin; swelling properties