title: Extraction, characterization and antioxidant activity of mycelial polysaccharides from Paecilomyces hepiali HN1.
abstract: Optimization of extraction, characterization and antioxidant activity of mycelial polysaccharides from Paecilomyces hepiali HN1 (PHMPs) were investigated. As results, the optimal parameters for extraction of PHMPs were obtained by a Box-Behnken design as follows: extraction temperature 92 °C, extracting time 190 min and ratio of water to material 43 mL/g. The analysis of monosaccharide composition by high performance liquid chromatography (HPLC) revealed that PHMPs was composed of mannose, ribose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose and arabinose in molar ratio of 46.07:0.59:2.25:1.29:1.42:18.82:26.17:1.13:2.26, respectively. Furthermore, it was demonstrated that PHMPs had a significant protective effect against oxidative stress induced by d-galactose in mice, as evident by higher activities of superoxide dismutase, catalase, glutathione peroxidase and level of total antioxidant capacity, as well as lower levels of malondialdehyde in serums and livers compared to the d-galactose-treated group. These results suggested that PHMPs could be explored as promising natural antioxidant.
authors.affiliation: College of Life Science and Technology, Henan Institute of Science and Technology, Xinxiang 453003, PR China; College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China; Collaborative Innovation Center of Modern Biological Breeding, Xinxiang 453003, Henan Province, PR China.
authors.forename: Zhongwei
authors.initials: Z
authors.lastname: Wu
authors.affiliation: College of Life Science and Technology, Henan Institute of Science and Technology, Xinxiang 453003, PR China; Collaborative Innovation Center of Modern Biological Breeding, Xinxiang 453003, Henan Province, PR China.
authors.forename: Mingxia
authors.initials: M
authors.lastname: Zhang
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Minhao
authors.initials: M
authors.lastname: Xie
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Zhuqing
authors.initials: Z
authors.lastname: Dai
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Wang
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Bing
authors.initials: B
authors.lastname: Hu
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Hong
authors.initials: H
authors.lastname: Ye
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Xiaoxiong
authors.initials: X
authors.lastname: Zeng
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology; D002374:Catalase / Q000378:metabolism; D005260:Female; D062610:Fungal Polysaccharides / Q000737:chemistry* / Q000494:pharmacology; D005979:Glutathione Peroxidase / Q000378:metabolism; D008315:Malondialdehyde / Q000097:blood / Q000378:metabolism; D051379:Mice; D009005:Monosaccharides / Q000032:analysis; D018384:Oxidative Stress; D010143:Paecilomyces / Q000737:chemistry*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D062610:Fungal Polysaccharides; D009005:Monosaccharides; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase
keywords: Antioxidant activity; Optimization; Paecilomyces hepiali HN1; Polysaccharides