title: Epidemiological analysis of serum anti-Pseudomonas aeruginosa PcrV titers in adults.
abstract: Of the various virulence mechanisms of the opportunistic pathogen Pseudomonas aeruginosa, the type III secretion system (TTSS) has been characterized as a major factor associated with acute lung injury, bacteremia and mortality. In addition, PcrV, a component protein of the TTSS, has been characterized as a protective antigen against infection with P. aeruginosa. This study comprised an epidemiological analysis of serum anti-PcrV titers in a cohort of Japanese adults. From April 2012 to March 2013, serum anti-PcrV titers of 198 volunteer participants undergoing anesthesia for scheduled surgeries were measured. The median, minimum and maximum serum anti-PcrV titers among the 198 participants were 4.09 nM, 1.01 nM and 113.81 nM, respectively. The maximum peaks in the histogram were within the anti-PcrV 2.00-4.99 nM titer range; values for 115 participants (58.1%) were within this range. Anti-PcrV titers were more than approximately three-fold greater (>12 nM) than the median value in 21 participants (10.6%). Ten-year interval age increases, history of treatment for traffic trauma, and a history of past surgery each showed statistically significant associations with higher anti-PcrV titers (i.e., >10 nM) than did the other factors assessed by binomial analysis. This study revealed a considerable variation in anti-PcrV titers in adult subjects without any obvious histories of infection with P. aeruginosa.
authors.affiliation: Department of Anesthesiology, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Yasumoto
authors.affiliation: Department of Anesthesiology, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan.
authors.forename: Hideya
authors.initials: H
authors.lastname: Katoh
authors.affiliation: Department of Anesthesiology, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan.
authors.forename: Mao
authors.initials: M
authors.lastname: Kinoshita
authors.affiliation: Department of Anesthesiology, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan.
authors.forename: Masaru
authors.initials: M
authors.lastname: Shimizu
authors.affiliation: Department of Anesthesiology, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan.
authors.forename: Saeko
authors.initials: S
authors.lastname: Hamaoka
authors.affiliation: Department of Anesthesiology, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan.
authors.forename: Koichi
authors.initials: K
authors.lastname: Akiyama
authors.affiliation: Department of Anesthesiology, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan.
authors.forename: Yoshifumi
authors.initials: Y
authors.lastname: Naito
authors.affiliation: Department of Anesthesiology, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan.
authors.forename: Teiji
authors.initials: T
authors.lastname: Sawa
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000907:Antibodies, Bacterial / Q000097:blood* / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000097:blood / Q000276:immunology; D000942:Antigens, Bacterial / Q000097:blood / Q000276:immunology; D016470:Bacteremia / Q000097:blood / Q000453:epidemiology / Q000276:immunology / Q000382:microbiology; D001427:Bacterial Toxins / Q000097:blood / Q000276:immunology; D015331:Cohort Studies; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D007564:Japan / Q000453:epidemiology; D008297:Male; D008875:Middle Aged; D052899:Pore Forming Cytotoxic Proteins / Q000097:blood / Q000276:immunology; D011552:Pseudomonas Infections / Q000097:blood / Q000453:epidemiology* / Q000276:immunology / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000276:immunology* / Q000302:isolation & purification; D000069296:Type III Secretion Systems / Q000276:immunology; D055815:Young Adult
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D001427:Bacterial Toxins; D007074:Immunoglobulin G; D052899:Pore Forming Cytotoxic Proteins; D000069296:Type III Secretion Systems; C118546:antigen V, Pseudomonas
keywords: PcrV; Pseudomonas aeruginosa; Type III secretion system; anti-PcrV titer