title: Evaluation of the immunogenicity and diagnostic interference caused by M. tuberculosis SO2 vaccination against tuberculosis in goats.
abstract: The immunogenicity and diagnostic interference caused by M. tuberculosis SO2, a prototype vaccine first time tested in goats was evaluated. Tuberculosis-free goats were distributed in four groups: [1], non-vaccinated; [2], subcutaneously (SC) BCG vaccinated; [3], intranasally (IN) SO2 vaccinated and [4], SC SO2 vaccinated. Intradermal tuberculin and IFN-γ tests using PPDs and alternative antigenic cocktails containing mainly ESAT-6 and CFP-10 (E/C) were applied at different times post-vaccination. Results showed a significant (p<0.05) increase in the number of reactors detected using both PPD-based intradermal and IFN-γ tests at different times in all the vaccinated groups. No intradermal reactivity was detected in the vaccinated goats using a cocktail containing E/C, Rv3615c and Rv3020c. A higher overall reactivity was observed in the group [4] in comparison with the other vaccinated groups. Results showed that antigens used to differentiate BCG vaccinated animals could be potentially used to differentiate SO2 vaccinated ones.
authors.affiliation: MAEVA SERVET S.L., Alameda del Valle, Madrid, Spain; Centro de Vigilancia Sanitaria Veterinaria (VISAVET), Universidad Complutense, Madrid, Spain. Electronic address: jbezosga@visavet.ucm.es.
authors.forename: Javier
authors.initials: J
authors.lastname: Bezos
authors.affiliation: Centro de Vigilancia Sanitaria Veterinaria (VISAVET), Universidad Complutense, Madrid, Spain.
authors.forename: Carmen
authors.initials: C
authors.lastname: Casal
authors.affiliation: BIOFABRI S.L., Porriño, Pontevedra, Spain.
authors.forename: Eugenia
authors.initials: E
authors.lastname: Puentes
authors.affiliation: MAEVA SERVET S.L., Alameda del Valle, Madrid, Spain.
authors.forename: Alberto
authors.initials: A
authors.lastname: Díez-Guerrier
authors.affiliation: Centro de Vigilancia Sanitaria Veterinaria (VISAVET), Universidad Complutense, Madrid, Spain.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Romero
authors.affiliation: Grupo de Genética de Micobacterias, Dpto. Microbiología, Medicina Preventiva y Salud Pública, Universidad de Zaragoza, Zaragoza, Spain; CIBER Enfermedades Respiratorias, Instituto de Salud Carlos III, Madrid, Spain.
authors.forename: Nacho
authors.initials: N
authors.lastname: Aguiló
authors.affiliation: Centro de Vigilancia Sanitaria Veterinaria (VISAVET), Universidad Complutense, Madrid, Spain; Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain.
authors.forename: Lucía
authors.initials: L
authors.lastname: de Juan
authors.affiliation: Grupo de Genética de Micobacterias, Dpto. Microbiología, Medicina Preventiva y Salud Pública, Universidad de Zaragoza, Zaragoza, Spain; CIBER Enfermedades Respiratorias, Instituto de Salud Carlos III, Madrid, Spain.
authors.forename: Carlos
authors.initials: C
authors.lastname: Martín
authors.affiliation: Centro de Vigilancia Sanitaria Veterinaria (VISAVET), Universidad Complutense, Madrid, Spain; Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain.
authors.forename: Lucas
authors.initials: L
authors.lastname: Domínguez
mesh_terms: D000818:Animals; D005260:Female; D015511:Goat Diseases / Q000382:microbiology / Q000635:transmission*; D006041:Goats; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D013030:Spain; D014376:Tuberculosis / Q000382:microbiology / Q000635:transmission / Q000662:veterinary*; D032581:Tuberculosis Vaccines / Q000276:immunology*; D014611:Vaccination
chemical_list: D032581:Tuberculosis Vaccines
keywords: DIVA; Diagnosis; Goat; Interference; Mycobacterium tuberculosis SO2; Vaccine