title: MicroRNA-130a expression is decreased in Xinjiang Uygur patients with type 2 diabetes mellitus.
abstract: OBJECTIVE
  MicroRNAs play important roles in energy metabolism, insulin synthesis, insulin transport and the development of diabetes. This study aims to investigate the expression and effect of microRNA-130a in Uygur patients with type 2 diabetes mellitus (T2DM).
  
  
  METHODS
  Peripheral blood and omental adipose tissues were collected from individuals with normal glucose tolerance and patients with T2DM. The microRNA expression profile of peripheral blood was established by microarray analysis. The differentially expressed microRNAs and possible target genes were identified by bioinformatics analysis. MicroRNA-130a mimics and inhibitors were transfected into 3T3-L1 preadipocytes.
  
  
  RESULTS
  Our results showed that microRNA-130a expression level was significantly decreased in peripheral blood and omental adipose tissues of T2DM patients (P < 0.01). Peroxisome proliferator-activated receptors γ (PPARγ) were predicted as target genes of microRNA-130a. This prediction was verified by the results that PPARγ mRNA expression in omental adipose tissues of T2DM patients were significantly increased (P < 0.01). The glucose consumption level after microRNA-130a transfection was significantly decreased (P < 0.05). And, microRNA-130a mimics inhibited PPARγ expression at both mRNA and protein level, further suggesting that PPARγ is a target gene of microRNA-130a. Additionally, adiponectin, lipoprotein lipase, CCAAT enhancer binding protein α, and the downstream genes of PPARγ, were significantly decreased after microRNA-130a mimics transfection.
  
  
  CONCLUSIONS
  In conclusion, microRNA-130a is decreased in Uygur patients with T2DM and it may play a role in T2DM through targeting PPARγ.
authors.affiliation: Department of Biochemistry and Molecular Biology, Preclinical Medicine College, Xinjiang Medical University Urumqi 830011, P. R China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Jiao
authors.affiliation: Central Laboratory of Xinjiang Medical University, Xinjiang Medical University Urumqi 830011, P. R China.
authors.forename: Manli
authors.initials: M
authors.lastname: Zhu
authors.affiliation: Traditional Chinese Medical College, Xinjiang Medical University Urumqi 830011, P. R China.
authors.forename: Xinmin
authors.initials: X
authors.lastname: Mao
authors.affiliation: Lab Center of Mechanism, Preclinical School, Xinjiang Medical University Urumqi 830011, P. R China.
authors.forename: Mei
authors.initials: M
authors.lastname: Long
authors.affiliation: Department of Pharmacology, Preclinical Medicine College, Xinjiang Medical University Urumqi, Xinjiang 830011, P. R China.
authors.forename: Xian
authors.initials: X
authors.lastname: Du
authors.affiliation: General Internal Medicine, First Affiliated Hospital of Xinjiang Medical University Urumqi 830011, P. R China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Endoscopic Branch, Xinjiang Uygur Autonomous Regional People's Hospital Urumqi 830000, P. R China.
authors.forename: Kelimu
authors.initials: K
authors.lastname: Abudureyimu
authors.affiliation: Endoscopic Branch, Xinjiang Uygur Autonomous Regional People's Hospital Urumqi 830000, P. R China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Pharmacology, Preclinical Medicine College, Xinjiang Medical University Urumqi, Xinjiang 830011, P. R China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Pharmacology, Preclinical Medicine College, Xinjiang Medical University Urumqi, Xinjiang 830011, P. R China.
authors.forename: Yicun
authors.initials: Y
authors.lastname: Tao
authors.affiliation: Department of Pharmacology, Preclinical Medicine College, Xinjiang Medical University Urumqi, Xinjiang 830011, P. R China.
authors.forename: Xin
authors.initials: X
authors.lastname: Luo
authors.affiliation: Department of Pharmacology, Preclinical Medicine College, Xinjiang Medical University Urumqi, Xinjiang 830011, P. R China.
authors.forename: Linlin
authors.initials: L
authors.lastname: Li
keywords: Uygur; microRNAs; microarray; peroxisome proliferator-activated receptors γ; tumor necrosis factor α; type 2 diabetes