title: Construction of Recombinant Single Chain Variable Fragment (ScFv) Antibody Against Superantigen for Immunodetection Using Antibody Phage Display Technology.
abstract: Superantigens are a class of antigens that bind to the major histocompatibility complex class (MHC) II and T-cell receptor (TCR) and cause the nonspecific activation of T cells, resulting in a massive release of pro-inflammatory mediators. They are produced by the gram-positive organisms Staphylococcus aureus and Streptococcus pyogenes, and by a variety of other microbes such as viruses and mycoplasma, and cause toxic shock syndrome (TSS) and even death in some cases. The immunodetection of superantigens is difficult due to the polyclonal activation of T-cells leading to nonspecific antibody production. The production of recombinant monoclonal antibodies against superantigens can solve this problem and are far better than polyclonal antibodies in terms of detection. Here, we describe the construction of recombinant single chain variable fragments (ScFv) antibodies against superantigens with specific reference to SEB (staphylococcal enterotoxin B) using antibody phage display technology.
authors.affiliation: Department of Ophthalmology, Anatomy and Cell Biology, Wayne State University School of Medicine, 4717 St. Antoine, Detroit, MI, 48201, USA. psing@med.wayne.edu.
authors.forename: Pawan Kumar
authors.initials: PK
authors.lastname: Singh
authors.affiliation: Biotechnology Division, Defense R&D Establishment, Jhansi Road, Gwalior, 474002, India.|Department of Chemistry, Chaudhary Charan Singh University, Meerut, 250004, India.
authors.forename: Ranu
authors.initials: R
authors.lastname: Agrawal
authors.affiliation: Biotechnology Division, Defense R&D Establishment, Jhansi Road, Gwalior, 474002, India. dvkamboj@gmail.com.
authors.forename: D V
authors.initials: DV
authors.lastname: Kamboj
authors.affiliation: Biotechnology Division, Defense R&D Establishment, Jhansi Road, Gwalior, 474002, India.
authors.forename: Lokendra
authors.initials: L
authors.lastname: Singh
mesh_terms: D000818:Animals; D015153:Blotting, Western; D061505:Cell Surface Display Techniques; D004797:Enzyme-Linked Immunosorbent Assay; D051379:Mice; D019151:Peptide Library; D010957:Plasmids / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics* / Q000276:immunology*; D057127:Single-Chain Antibodies / Q000235:genetics* / Q000276:immunology*; D013211:Staphylococcus aureus / Q000276:immunology*; D018089:Superantigens / Q000276:immunology*
chemical_list: D019151:Peptide Library; D011993:Recombinant Fusion Proteins; D057127:Single-Chain Antibodies; D018089:Superantigens
keywords: Bacteriophages; Biopanning; Phage display; Recombinant antibody; SEB; ScFv; Superantigen