title: BPA exposure during in vitro oocyte maturation results in dose-dependent alterations to embryo development rates, apoptosis rate, sex ratio and gene expression.
abstract: Alterations in the oocyte's environment can negatively affect embryo development. Oocyte quality, which can determine embryonic viability, is easily perturbed, thus factors affecting normal oocyte maturation are a concern. Bisphenol A (BPA) is an endocrine disrupting chemical that elicits a variety of reproductive effects. BPA has previously been found to disrupt meiosis, however the embryonic effects in mammals are not well documented. Here, bovine oocytes were matured in vitro with and without BPA treatment. Resulting embryos exhibited decreased embryonic development rates, increased apoptosis, and a skewed sex ratio. Gene expression in blastocysts was not altered, whereas treatment with 15ng/mL BPA resulted in increased expression of several of the genes studies, however this increase was largely due to a vehicle effect. BPA exposure during oocyte maturation in vitro can therefore, in a dose-dependent way, decrease oocyte and embryo quality and developmental potential and affect gene expression of developmentally important transcripts.
authors.affiliation: University of Guelph, Department of Biomedical Sciences, 50 Stone Road E, Guelph, ON N1G 2W1 Canada.
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Ferris
authors.affiliation: University of Guelph, Department of Biomedical Sciences, 50 Stone Road E, Guelph, ON N1G 2W1 Canada.
authors.forename: Kiana
authors.initials: K
authors.lastname: Mahboubi
authors.affiliation: University of Guelph, Department of Biomedical Sciences, 50 Stone Road E, Guelph, ON N1G 2W1 Canada.
authors.forename: Neil
authors.initials: N
authors.lastname: MacLusky
authors.affiliation: University of Guelph, Department of Biomedical Sciences, 50 Stone Road E, Guelph, ON N1G 2W1 Canada.
authors.forename: W Allan
authors.initials: WA
authors.lastname: King
authors.affiliation: University of Guelph, Department of Biomedical Sciences, 50 Stone Road E, Guelph, ON N1G 2W1 Canada. Electronic address: lfavetta@uoguelph.ca.
authors.forename: Laura A
authors.initials: LA
authors.lastname: Favetta
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001559:Benzhydryl Compounds / Q000633:toxicity*; D001755:Blastocyst / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D002417:Cattle; D002478:Cells, Cultured; D052244:Endocrine Disruptors / Q000633:toxicity*; D004785:Environmental Pollutants / Q000633:toxicity*; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects*; D059471:In Vitro Oocyte Maturation Techniques; D008297:Male; D009865:Oocytes / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D010636:Phenols / Q000633:toxicity*; D011247:Pregnancy; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012744:Sex Ratio; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D052244:Endocrine Disruptors; D004785:Environmental Pollutants; D010636:Phenols; D012333:RNA, Messenger; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: Apoptosis; Bisphenol A; Embryo development; Gene expression; Sex ratio; Stress