title: Specific and selective electrochemical immunoassay for Pseudomonas aeruginosa based on pectin-gold nano composite.
abstract: In this report, we have successfully fabricated an immunosensor for detection of Pseudomonas aeruginosa in water. The monoclonal antibody was immobilized on the surface modified with CCLP (Calcium Cross-Linked Pectin)-Au NPs (gold nanoparticles)/Glassy Carbon Electrode. The building of the immunosensor was evaluated in each step by cyclic voltammetry (CV) and impedance spectroscopy (EIS). The electrochemical detection was done based on the anti rabbit IgG HRP (Horseradish Peroxidase) which binds to the immune complex and the response was observed using Hydro Quininone (HQ) and Hydrogen peroxide (H2O2) in PB (Phosphate Buffer) electrolyte. From the results, the sensitivity range is from 10(1) to 10(7)CFU/ml and LOD is calculated as 9×10(2)CFU/ml. The developed immunosensor also have high selectivity, stability, reproducibility and reusability.
authors.affiliation: Department of Plant Biotechnology, School of Biotechnology, Madurai Kamaraj University, Madurai, India.
authors.forename: N
authors.initials: N
authors.lastname: Krithiga
authors.affiliation: Department of Natural Product Chemistry, School of Chemistry, Madurai Kamaraj University, Madurai, India.
authors.forename: K Balaji
authors.initials: KB
authors.lastname: Viswanath
authors.affiliation: Department of Natural Product Chemistry, School of Chemistry, Madurai Kamaraj University, Madurai, India. Electronic address: Vasantham999@yahoo.co.in.
authors.forename: V S
authors.initials: VS
authors.lastname: Vasantha
authors.affiliation: Department of Plant Biotechnology, School of Biotechnology, Madurai Kamaraj University, Madurai, India. Electronic address: jchitralab@gmail.com.
authors.forename: A
authors.initials: A
authors.lastname: Jayachitra
mesh_terms: D000818:Animals; D055910:Antibodies, Immobilized / Q000737:chemistry; D058266:Dielectric Spectroscopy / Q000379:methods; D055664:Electrochemical Techniques / Q000379:methods; D006046:Gold / Q000737:chemistry*; D006735:Horseradish Peroxidase / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000737:chemistry; D007118:Immunoassay / Q000379:methods; D057230:Limit of Detection; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D010368:Pectins / Q000737:chemistry*; D011550:Pseudomonas aeruginosa / Q000302:isolation & purification*; D011817:Rabbits; D015203:Reproducibility of Results; D014871:Water Microbiology
chemical_list: D055910:Antibodies, Immobilized; D006046:Gold; D010368:Pectins; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase
keywords: CCLP-Au NPs; Hydrogen peroxide; Hydroquinone; IgG-HRP; Phosphate buffer; Pseudomonas aeruginosa