title: Thermoresponsive amperometric glucose biosensor.
abstract: The authors report on the fabrication of a thermoresponsive biosensor for the amperometric detection of glucose. Screen printed electrodes with heatable gold working electrodes were modified by a thermoresponsive statistical copolymer [polymer I: poly(ω-ethoxytriethylenglycol methacrylate-co-3-(N,N-dimethyl-N-2-methacryloyloxyethyl ammonio) propanesulfonate-co-ω-butoxydiethylenglycol methacrylate-co-2-(4-benzoyl-phenoxy)ethyl methacrylate)] with a lower critical solution temperature of around 28 °C in aqueous solution via electrochemically induced codeposition with a pH-responsive redox-polymer [polymer II: poly(glycidyl methacrylate-co-allyl methacrylate-co-poly(ethylene glycol)methacrylate-co-butyl acrylate-co-2-(dimethylamino)ethyl methacrylate)-[Os(bpy)2(4-(((2-(2-(2-aminoethoxy)ethoxy)ethyl)amino)methyl)-N,N-dimethylpicolinamide)](2+)] and pyrroloquinoline quinone-soluble glucose dehydrogenase acting as biological recognition element. Polymer II bears covalently bound Os-complexes that act as redox mediators for shuttling electrons between the enzyme and the electrode surface. Polymer I acts as a temperature triggered immobilization matrix. Probing the catalytic current as a function of the working electrode temperature shows that the activity of the biosensor is dramatically reduced above the phase transition temperature of polymer I. Thus, the local modulation of the temperature at the interphase between the electrode and the bioactive layer allows switching the biosensor from an on- to an off-state without heating of the surrounding analyte solution.
authors.affiliation: Analytical Chemistry - Center for Electrochemical Sciences (CES), Ruhr-Universität Bochum, Universitätsstr. 150, D-44780 Bochum, Germany.
authors.forename: Piyanut
authors.initials: P
authors.lastname: Pinyou
authors.affiliation: Analytical Chemistry - Center for Electrochemical Sciences (CES), Ruhr-Universität Bochum, Universitätsstr. 150, D-44780 Bochum, Germany.
authors.forename: Adrian
authors.initials: A
authors.lastname: Ruff
authors.affiliation: Analytical Chemistry - Center for Electrochemical Sciences (CES), Ruhr-Universität Bochum, Universitätsstr. 150, D-44780 Bochum, Germany.
authors.forename: Sascha
authors.initials: S
authors.lastname: Pöller
authors.affiliation: Analytical Chemistry - Center for Electrochemical Sciences (CES), Ruhr-Universität Bochum, Universitätsstr. 150, D-44780 Bochum, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Barwe
authors.affiliation: Analytical Chemistry - Center for Electrochemical Sciences (CES), Ruhr-Universität Bochum, Universitätsstr. 150, D-44780 Bochum, Germany.
authors.forename: Michaela
authors.initials: M
authors.lastname: Nebel
authors.affiliation: Fraunhofer Institute for Applied Polymer Research, Geiselbergstrasse 69, D-14476 Potsdam-Golm, Germany.
authors.forename: Natalia Guerrero
authors.initials: NG
authors.lastname: Alburquerque
authors.affiliation: Fraunhofer Institute for Applied Polymer Research, Geiselbergstrasse 69, D-14476 Potsdam-Golm, Germany.
authors.forename: Erik
authors.initials: E
authors.lastname: Wischerhoff
authors.affiliation: Institut für Chemie, Universität Potsdam, Karl-Liebknecht-Str. 24-25, D-14476 Potsdam-Golm, Germany.
authors.forename: André
authors.initials: A
authors.lastname: Laschewsky
authors.affiliation: BST BioSensor Technology GmbH, Buchholzer Str. 58, D-13156 Berlin, Germany.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Schmaderer
authors.affiliation: BST BioSensor Technology GmbH, Buchholzer Str. 58, D-13156 Berlin, Germany.
authors.forename: Jan
authors.initials: J
authors.lastname: Szeponik
authors.affiliation: Center for Electrochemical Sciences (CES) - Molecular Nanostructures, Ruhr-Universität Bochum, Universitätsstr. 150, D-44780 Bochum, Germany.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Plumeré
authors.affiliation: Analytical Chemistry - Center for Electrochemical Sciences (CES), Ruhr-Universität Bochum, Universitätsstr. 150, D-44780 Bochum, Germany.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Schuhmann
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D055664:Electrochemical Techniques / Q000379:methods*; D005947:Glucose / Q000032:analysis*; D013696:Temperature
chemical_list: D005947:Glucose