title: Expression of DNAM-1 (CD226) on inflammatory monocytes.
abstract: DNAM-1 is an activating receptor expressed on NK cells and T cells and plays an important role in cytotoxicity of these cells against target cells. Although the role of DNAM-1 in the function of T cells and NK cells has been well studied, the expression and function of DNAM-1 on myeloid cells have been incompletely understood. In this study, we investigated expression of DNAM-1 on monocyte subsets in mouse peripheral blood and found that only inflammatory monocytes (iMos), but not patrolling monocytes (pMos), expressed high levels of DNAM-1. In addition, we found that DNAM-1 was highly expressed on iMos, rather than pMos, also in human. Furthermore, we found that DNAM-1 on inflammatory monocytes was involved in cell adhesion to CD155-expressing cells. Therefore, we propose that expression of DNAM-1 on inflammatory monocytes are evolutionally conserved and act as an adhesion molecule on blood inflammatory monocytes.
authors.affiliation: Department of Immunology, Faculty of Medicine, Japan; Human Biology Program, School of Integrative and Global Majors, Japan.
authors.forename: Anh Van
authors.initials: AV
authors.lastname: Vo
authors.affiliation: Department of Immunology, Faculty of Medicine, Japan.
authors.forename: Eri
authors.initials: E
authors.lastname: Takenaka
authors.affiliation: Department of Immunology, Faculty of Medicine, Japan; Life Science Center of Tsukuba Advanced Research Alliance (TARA), Japan; Japan Science and Technology Agency, Core Research for Evolutional Science and Technology (CREST), University of Tsukuba, 1-1-1, Tennohdai, Tsukuba, Ibaraki 305-8575, Japan; AMED-CREST, AMED, Japan Agency for Medical Research and Development, 1-7-1 Otemachi, Chiyodaku, Tokyo 100-0004, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Shibuya
authors.affiliation: Department of Immunology, Faculty of Medicine, Japan. Electronic address: kazukos@md.tsukuba.ac.jp.
authors.forename: Kazuko
authors.initials: K
authors.lastname: Shibuya
mesh_terms: D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte / Q000096:biosynthesis* / Q000276:immunology; D002448:Cell Adhesion / Q000276:immunology*; D015815:Cell Adhesion Molecules / Q000096:biosynthesis* / Q000276:immunology; D005434:Flow Cytometry; D006801:Humans; D007249:Inflammation / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009000:Monocytes / Q000276:immunology*; D000098862:T Lineage-Specific Activation Antigen 1
chemical_list: D000945:Antigens, Differentiation, T-Lymphocyte; D000098862:T Lineage-Specific Activation Antigen 1; D015815:Cell Adhesion Molecules
keywords: Adhesion; DNAM-1 (CD226); Inflammatory monocytes; Patrolling monocytes