title: Study on pharmacokinetics of 3,4-divanillyltetrahydrofuran in rats by ultra-fast liquid chromatography/tandem mass spectrometry.
abstract: 3,4-Divanillyltetrahydrofuran is the main active ingredient of nettle root which can increase steroid hormones in the bloodstream for many of bodybuilders. To better understand its pharmacological activities, we need to determine its pharmacokinetic profiles. In this study, a rapid and sensitive ultra-fast liquid chromatography-tandem mass spectrometry (UFLC-MS/MS) method has been developed for the determination of 3,4-divanillyltetrahydrofuran in the plasma of rats. Chromatographic separation was performed on a C18 column at 40°C, with a gradient elution consisting of methanol and water containing 0.3% (v/v) formic acid at a flow rate of 0.8mL/min. The detection was performed using an electrospray triple-quadrupole MS/MS via positive ion multiple reaction monitoring mode. The lower limits-of-quantification determined were 0.5ng/mL. The intra- and inter-day precision (RSD%) was found to be within 15% and the accuracy (RE%) ranged from -4.0% to 7.0%. This simple yet sensitive method was fully validated and could be successfully applied to the study on pharmacokinetics of 3, 4-divanillyltetrahydrofuran.
authors.affiliation: School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, People's Republic of China.
authors.forename: Chen-Xiao
authors.initials: CX
authors.lastname: Shan
authors.affiliation: School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, People's Republic of China. Electronic address: xiaobingcui@163.com.
authors.forename: Xiao-Bing
authors.initials: XB
authors.lastname: Cui
authors.affiliation: School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, People's Republic of China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Yu
authors.affiliation: School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, People's Republic of China.
authors.forename: Chuan
authors.initials: C
authors.lastname: Chai
authors.affiliation: School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, People's Republic of China.
authors.forename: Hong-Mei
authors.initials: HM
authors.lastname: Wen
authors.affiliation: School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, People's Republic of China.
authors.forename: Xin-Zhi
authors.initials: XZ
authors.lastname: Wang
authors.affiliation: School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, People's Republic of China.
authors.forename: Xue
authors.initials: X
authors.lastname: Sun
mesh_terms: D000818:Animals; D019540:Area Under Curve; D002853:Chromatography, Liquid / Q000379:methods*; D005663:Furans / Q000097:blood / Q000493:pharmacokinetics*; D006207:Half-Life; D008031:Lignin / Q000097:blood / Q000493:pharmacokinetics*; D008297:Male; D051381:Rats; D012015:Reference Standards; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: C110595:3,4-divanillyltetrahydrofuran; D005663:Furans; D008031:Lignin
keywords: 3,4-Divanillyltetrahydrofuran; Pharmacokinetics; Rat plasma; UFLC–MS/MS