title: Different Sialoside Epitopes on Collagen Film Surfaces Direct Mesenchymal Stem Cell Fate.
abstract: 3'-Sialyllactose and 6'-sialyllactose have been covalently linked to collagen films. Preliminary in vitro study on the behavior of mesenchymal stem cells (MSCs) in terms of cell viability, proliferation and induction of osteogenic and chondrogenic related genes has been performed. Results indicate that sialoside epitopes on collagen surface represent a suitable support for MSCs adhesion and cell proliferation, moreover, the neoglycosylation provide MSCs with different and specific stimuli, saccharide-type depending, in term of expression of osteogenic and chondrogenic related genes. In particular, 3'-sialyllactose significantly upregulate the expression of RUNX2 and ALP, well-known markers of osteogenesis, whereas 6'-sialyllactose up-regulate the expression of chondrocyte marker ACAN. Because no osteogenic or chondrogenic supplements in culture media were added, the inductive effect in terms of increased gene expression has to be ascribed uniquely to collagen surface functionalization. These results support the promising role of sialosides in the regulation of stem cells fate and open brilliant perspective for the future use of the presented approach toward osteochondral tissue engineering applications.
authors.affiliation: Department of Biotechnology and Biosciences, University of Milano-Bicocca , Piazza della Scienza 2, I-20126 Milano, Italy.
authors.forename: Antonella
authors.initials: A
authors.lastname: Sgambato
authors.affiliation: Department of Biotechnology and Biosciences, University of Milano-Bicocca , Piazza della Scienza 2, I-20126 Milano, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Russo
authors.affiliation: Bioceramics and Bio-hybrid Composites Group, Institute of Science and Technology for Ceramics, National Research Council , Via Granarolo 64, 48018 Faenza, Ravenna, Italy.
authors.forename: Monica
authors.initials: M
authors.lastname: Montesi
authors.affiliation: Bioceramics and Bio-hybrid Composites Group, Institute of Science and Technology for Ceramics, National Research Council , Via Granarolo 64, 48018 Faenza, Ravenna, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Panseri
authors.affiliation: Laboratory of Biomechanics and Technology Innovation, Rizzoli Orthopaedic Institute , via di Barbiano, 1/10, 40136 Bologna, Italy.
authors.forename: Maurilio
authors.initials: M
authors.lastname: Marcacci
authors.affiliation: Department of Surgical and Morphological Sciences, Insubria University , Via Guicciardini 9, 21100 Varese, Italy.
authors.forename: Elena
authors.initials: E
authors.lastname: Caravà
authors.affiliation: Department of Surgical and Morphological Sciences, Insubria University , Via Guicciardini 9, 21100 Varese, Italy.
authors.forename: Mario
authors.initials: M
authors.lastname: Raspanti
authors.forename: Laura
authors.initials: L
authors.lastname: Cipolla
mesh_terms: D002454:Cell Differentiation; D002478:Cells, Cultured; D020219:Chondrogenesis; D003094:Collagen; D000939:Epitopes; D059630:Mesenchymal Stem Cells; D010012:Osteogenesis
chemical_list: D000939:Epitopes; D003094:Collagen
keywords: carbohydrates; chondrogenesis; collagen; mesenchymal stem cells; osteogenesis; sialic acid