title: Consumption of onion juice modulates oxidative stress and attenuates the risk of bone disorders in middle-aged and post-menopausal healthy subjects.
abstract: Osteoporosis is a chronic inflammatory condition that is characterized by the loss of bone mineral density (BMD). The current study was undertaken to evaluate the impact of onion juice intake on the bone mineral density (BMD) and bone loss in corroboration with antioxidant effects in human (in vivo) as well as inhibitory effects on the differentiation of osteoclasts in the cell line (in vitro). For in vitro studies, the RAW 264.7 (osteoclast progenitor) cells were used to examine the anti-osteoclastogenic effect of onion. In the case of in vivo studies, twenty-four subjects were divided into two groups and advised to intake 100 mL of onion juice or placebo for 8 weeks. Anthropometric measurements and blood samples were collected at the initial, 2(nd), 6(th), 8(th) and 10(th) week. The result of in vitro studies indicated that onion extract would effectively inhibit the osteoclastogenesis and its differentiation. Significant changes in the levels of alkaline phosphatase (ALP), free radicals, total antioxidant capacity (TEAC) and various antioxidants were observed in onion administered subjects. The BMD of three postmenopausal women was also found to be mildly improved on supplementation with onion juice. Onion juice consumption showed a positive modulatory effect on the bone loss and BMD by improving antioxidant activities and thus can be recommended for treating various bone-related disorders, especially osteoporosis.
authors.affiliation: Department of Orthopedics, Chung Shan Medical University Hospital, Institute of Medicine, Chung Shan Medical University, 110, Sec. 1, Jianguo North Road, Taichung City, Taiwan, Republic of China.
authors.forename: Yat-Yin
authors.initials: YY
authors.lastname: Law
authors.affiliation: Department of Chinese Medicine, Taichung Hospital Ministry of Health and Well-being, Taichung, Taiwan, Republic of China.
authors.forename: Hui-Fang
authors.initials: HF
authors.lastname: Chiu
authors.affiliation: School of Nutrition, Chung Shan Medical University, 110, Sec. 1, Jianguo North Road, Taichung City, Taiwan, Republic of China. wck@csmu.edu.tw.
authors.forename: Hui-Hsin
authors.initials: HH
authors.lastname: Lee
authors.affiliation: School of Health Diet and Industry Management, Chung Shan Medical University, 110, Sec. 1, Jianguo North Road, Taichung City, Taiwan, Republic of China.
authors.forename: You-Cheng
authors.initials: YC
authors.lastname: Shen
authors.affiliation: School of Nutrition, Chung Shan Medical University, 110, Sec. 1, Jianguo North Road, Taichung City, Taiwan, Republic of China. wck@csmu.edu.tw.
authors.forename: Kamesh
authors.initials: K
authors.lastname: Venkatakrishnan
authors.affiliation: School of Nutrition, Chung Shan Medical University, 110, Sec. 1, Jianguo North Road, Taichung City, Taiwan, Republic of China. wck@csmu.edu.tw.
authors.forename: Chin-Kun
authors.initials: CK
authors.lastname: Wang
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D015519:Bone Density; D002118:Calcium / Q000097:blood; D002454:Cell Differentiation; D002470:Cell Survival; D004311:Double-Blind Method; D005260:Female; D005419:Flavonoids / Q000032:analysis; D000067030:Fruit and Vegetable Juices; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D008297:Male; D051379:Mice; D008875:Middle Aged; D019697:Onions / Q000737:chemistry*; D010012:Osteogenesis / Q000502:physiology; D015663:Osteoporosis, Postmenopausal / Q000517:prevention & control*; D018384:Oxidative Stress / Q000502:physiology*; D010281:Parathyroid Hormone / Q000097:blood; D010636:Phenols / Q000032:analysis; D010758:Phosphorus / Q000097:blood; D000067996:RAW 264.7 Cells; D012307:Risk Factors
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D010281:Parathyroid Hormone; D010636:Phenols; D010758:Phosphorus; D006861:Hydrogen Peroxide; D002118:Calcium