title: Acceleration of hard and soft tissue healing in the oral cavity by a single transmucosal injection of fluvastatin-impregnated poly (lactic-co-glycolic acid) microspheres. An in vitro and rodent in vivo study.
abstract: Antihyperlipidemic drug statins reportedly promote both bone formation and soft tissue healing. We examined the effect of sustained-release, fluvastatin-impregnated poly(lactic-co-glycolic acid) (PLGA) microspheres on the promotion of bone and gingival healing at an extraction socket in vivo, and the effect of fluvastatin on epithelial cells and fibroblasts in vitro. The maxillary right first molar was extracted in rats, then one of the following was immediately injected, as a single dose, into the gingivobuccal fold: control (no administration), PLGA microspheres without a statin (active control), or PLGA microspheres containing 20 or 40 μg kg(-1) of fluvastatin. At days 1, 3, 7, 14, and 28 after injection, bone and soft tissue healing were histologically evaluated. Cell proliferation was measured under the effect of fluvastatin at dosages of 0, 0.01, 0.1, 1.0, 10, and 50 μM. Cell migration and morphology were observed at dosages of 0 and 0.1 μM. Following tooth extraction, the statin significantly enhanced bone volume and density, connective tissue volume, and epithelial wound healing. In the in vitro study, it promoted significant proliferation and migration of epithelial cells and fibroblasts. A single dose of topically administered fluvastatin-impregnated PLGA microspheres promoted bone and soft tissue healing at the extraction site.
authors.affiliation: Section of Implant and Rehabilitative Dentistry, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Yasunami
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Ayukawa
authors.forename: Akihiro
authors.initials: A
authors.lastname: Furuhashi
authors.forename: Ikiru
authors.initials: I
authors.lastname: Atsuta
authors.forename: Yunia Dwi
authors.initials: YD
authors.lastname: Rakhmatia
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Moriyama
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Masuzaki
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Koyano
mesh_terms: D065966:Absorption, Physicochemical; D000818:Animals; D000924:Anticholesteremic Agents / Q000008:administration & dosage; D001846:Bone Development / Q000187:drug effects; D002214:Capsules / Q000008:administration & dosage / Q000138:chemical synthesis*; D003238:Connective Tissue / Q000187:drug effects / Q000254:growth & development; D004058:Diffusion; D005229:Fatty Acids, Monounsaturated / Q000008:administration & dosage* / Q000737:chemistry; D000077340:Fluvastatin; D066298:In Vitro Techniques; D007211:Indoles / Q000008:administration & dosage* / Q000737:chemistry; D019344:Lactic Acid / Q000737:chemistry*; D008422:Materials Testing; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D051381:Rats; D017208:Rats, Wistar; D020390:Tooth Socket / Q000187:drug effects* / Q000473:pathology*; D016896:Treatment Outcome; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000924:Anticholesteremic Agents; D002214:Capsules; D005229:Fatty Acids, Monounsaturated; D007211:Indoles; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D000077340:Fluvastatin