title: Development of GRAS strains for nutraceutical production using systems and synthetic biology approaches: advances and prospects.
abstract: Nutraceuticals are food substances with medical and health benefits for humans. Limited by complicated procedures, high cost, low yield, insufficient raw materials, resource waste, and environment pollution, chemical synthesis and extraction are being replaced by microbial synthesis of nutraceuticals. Many microbial strains that are generally regarded as safe (GRAS) have been identified and developed for the synthesis of nutraceuticals, and significant nutraceutical production by these strains has been achieved. In this review, we systematically summarize recent advances in nutraceutical research in terms of physiological effects on health, potential applications, drawbacks of traditional production processes, characteristics of production strains, and progress in microbial fermentation. Recent advances in systems and synthetic biology techniques have enabled comprehensive understanding of GRAS strains and its wider applications. Thus, these microbial strains are promising cell factories for the commercial production of nutraceuticals.
authors.affiliation: a Key Laboratory of Carbohydrate Chemistry and Biotechnology and.|b Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University , Wuxi , China.|c Synergetic Innovation of Center of Food Safety and Nutrition, Jiangnan University , Wuxi , China , and.
authors.forename: Long
authors.initials: L
authors.lastname: Liu
authors.affiliation: a Key Laboratory of Carbohydrate Chemistry and Biotechnology and.|c Synergetic Innovation of Center of Food Safety and Nutrition, Jiangnan University , Wuxi , China , and.
authors.forename: Ningzi
authors.initials: N
authors.lastname: Guan
authors.affiliation: a Key Laboratory of Carbohydrate Chemistry and Biotechnology and.|b Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University , Wuxi , China.
authors.forename: Jianghua
authors.initials: J
authors.lastname: Li
authors.affiliation: d School of Chemical and Biomolecular Engineering, Georgia Institute of Technology , Atlanta , GA , USA.
authors.forename: Hyun-Dong
authors.initials: HD
authors.lastname: Shin
authors.affiliation: a Key Laboratory of Carbohydrate Chemistry and Biotechnology and.|b Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University , Wuxi , China.|c Synergetic Innovation of Center of Food Safety and Nutrition, Jiangnan University , Wuxi , China , and.
authors.forename: Guocheng
authors.initials: G
authors.lastname: Du
authors.affiliation: a Key Laboratory of Carbohydrate Chemistry and Biotechnology and.|b Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University , Wuxi , China.|c Synergetic Innovation of Center of Food Safety and Nutrition, Jiangnan University , Wuxi , China , and.
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
mesh_terms: D001419:Bacteria / Q000378:metabolism; D019587:Dietary Supplements; D005658:Fungi / Q000378:metabolism; D058615:Synthetic Biology; D049490:Systems Biology
keywords: GRAS strain; microbial cell factory; nutraceuticals; synthetic biology; systems biology