title: Characterization and multiplexing of 21 microsatellite markers for the herb Noccaea caerulescens (Brassicaceae).
abstract: OBJECTIVE
  Multiplexed microsatellite markers were developed for population genetic studies in the pseudometallophyte Noccaea caerulescens (Brassicaceae), a model species to investigate metal tolerance and hyperaccumulation in higher plants.
  
  
  RESULTS
  Microsatellite loci were isolated through pyrosequencing of an enriched DNA library. Three multiplexes combining four previously published and 17 newly designed markers were developed. The new markers were screened in metallicolous and nonmetallicolous populations from southern France. The total number of alleles per locus ranged from five to 18. The observed heterozygosity per locus and per population ranged from 0 to 0.83, and expected heterozygosity ranged from 0 to 0.89.
  
  
  CONCLUSIONS
  The investigated loci showed reasonable to high levels of polymorphism at the regional scale. The multiplex set should be helpful in investigating genetic diversity, population structure, and demographic history in N. caerulescens at various spatial scales.
authors.affiliation: Institut des Sciences de l'Évolution, Université de Montpellier, CNRS, IRD, EPHE, CC 065, Place Eugène Bataillon 34095, Montpellier CEDEX 05, France.
authors.forename: Mathilde
authors.initials: M
authors.lastname: Mousset
authors.affiliation: Institut des Sciences de l'Évolution, Université de Montpellier, CNRS, IRD, EPHE, CC 065, Place Eugène Bataillon 34095, Montpellier CEDEX 05, France.
authors.forename: Elodie
authors.initials: E
authors.lastname: Flaven
authors.affiliation: Institut des Sciences de l'Évolution, Université de Montpellier, CNRS, IRD, EPHE, CC 065, Place Eugène Bataillon 34095, Montpellier CEDEX 05, France.
authors.forename: Fabienne
authors.initials: F
authors.lastname: Justy
authors.affiliation: Institut des Sciences de l'Évolution, Université de Montpellier, CNRS, IRD, EPHE, CC 065, Place Eugène Bataillon 34095, Montpellier CEDEX 05, France.
authors.forename: Juliette
authors.initials: J
authors.lastname: Pouzadoux
authors.affiliation: Université Lille, CNRS, UMR 8198, Evo-Eco-Paleo, F-59000 Lille, France.
authors.forename: Cécile
authors.initials: C
authors.lastname: Gode
authors.affiliation: Université Lille, CNRS, UMR 8198, Evo-Eco-Paleo, F-59000 Lille, France.
authors.forename: Maxime
authors.initials: M
authors.lastname: Pauwels
authors.affiliation: Université de Lorraine, Laboratoire Sols et Environnement (UMR 1120), F-54518 Vandœuvre-lès-Nancy CEDEX, France ; INRA, Laboratoire Sols et Environnement (UMR 1120), F-54518 Vandœuvre-lès-Nancy CEDEX, France.
authors.forename: Cédric
authors.initials: C
authors.lastname: Gonneau
keywords: Brassicaceae; Noccaea caerulescens; heavy metal tolerance; microsatellite; pseudometallophyte