title: Loop-mediated Isothermal Amplification Assay to Rapidly Detect Wheat Streak Mosaic Virus in Quarantined Plants.
abstract: We developed a loop-mediated isothermal amplification (LAMP) method to rapidly diagnose Wheat streak mosaic virus (WSMV) during quarantine inspections of imported wheat, corn, oats, and millet. The LAMP method was developed as a plant quarantine inspection method for the first time, and its simplicity, quickness, specificity and sensitivity were verified compared to current reverse transcription-polymerase chain reaction (RT-PCR) and nested PCR quarantine methods. We were able to quickly screen for WSMV at quarantine sites with many test samples; thus, this method is expected to contribute to plant quarantine inspections.
authors.affiliation: Water Supply and Sewerage Research Division, National Institute of Environmental Research, Incheon 404-708, Korea.
authors.forename: Siwon
authors.initials: S
authors.lastname: Lee
authors.affiliation: Department of Chemistry, College of Natural Science, Dankook University, Cheonan 330-714, Korea.
authors.forename: Jin-Ho
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Department of Chemistry, College of Natural Science, Dankook University, Cheonan 330-714, Korea ; Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan 330-714, Korea.
authors.forename: Ji-Young
authors.initials: JY
authors.lastname: Choi
authors.affiliation: Department of Chemistry, College of Natural Science, Dankook University, Cheonan 330-714, Korea.
authors.forename: Won-Cheoul
authors.initials: WC
authors.lastname: Jang
keywords: LAMP; WSMV; loop-mediated isothermal amplification; quarantine; wheat streak mosaic virus