title: Differential in situ sensing of extra- and intracellular glutathione by a novel redox-responsive silica matrix-Au nanoprobe.
abstract: We synthesized a biothiol-sensitive nanoprobe by assembling gold nanoparticles with a novel redox-responsive silica (ReSi) matrix using dithiobis (succinimidyl propionate) and (3-aminopropyl) trimethoxysilane. Thin layer disulfide-bonded networks of the ReSi could differentially respond to extra- and intracellular glutathione in cancer cells within 30 min; furthermore, targeted cellular uptake could be monitored in situ by fluorescence recovery. Sigmoidal dose-response pattern of the nanoprobes presented in this study were attributed to the buried disulfide-linked 3D nanostructure of the ReSi nanoshell, optimized at an appropriate thickness, enabling not only buffering of small redox disturbances in the extracellular milieu but also the satisfied sensitivity for rapid redox sensing. Such a ReSi-functionalized gold nanoparticle-based nanoconjugate possesses the potential to serve as an effective intracellular drug carrier for future cancer theranostics.
authors.affiliation: Department of Applied Chemistry, National Chiao-Tung University, No.1001 Ta-Hsueh Road, Hsinchu 30010, Taiwan, ROC.
authors.forename: Yun-Ling
authors.initials: YL
authors.lastname: Luo
authors.affiliation: Department of Applied Chemistry, National Chiao-Tung University, No.1001 Ta-Hsueh Road, Hsinchu 30010, Taiwan, ROC.
authors.forename: Xin-Chun
authors.initials: XC
authors.lastname: Huang
authors.affiliation: Department of Applied Chemistry, National Chiao-Tung University, No.1001 Ta-Hsueh Road, Hsinchu 30010, Taiwan, ROC.
authors.forename: Wei-Ming
authors.initials: WM
authors.lastname: Tu
authors.affiliation: Department of Applied Chemistry, National Chiao-Tung University, No.1001 Ta-Hsueh Road, Hsinchu 30010, Taiwan, ROC; Institute of Molecular Science, National Chiao-Tung University, No.1001 Ta-Hsueh Road, Hsinchu 30010, Taiwan, ROC. Electronic address: hyhsu99@nctu.edu.tw.
authors.forename: Hsin-Yun
authors.initials: HY
authors.lastname: Hsu
mesh_terms: D005453:Fluorescence; D005978:Glutathione / Q000032:analysis*; D006046:Gold / Q000737:chemistry*; D056945:Hep G2 Cells; D006801:Humans; D053768:Metal Nanoparticles; D015335:Molecular Probes; D010084:Oxidation-Reduction; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D015335:Molecular Probes; D006046:Gold; D012822:Silicon Dioxide; D005978:Glutathione
keywords: Cancer bio-sensing; Glutathione; Gold nanoparticle; Redox-responsive silica matrix