title: Draft genome sequence of the silver pomfret fish, Pampus argenteus.
abstract: Silver pomfret, Pampus argenteus, is a fish species from coastal waters. Despite its high commercial value, this edible fish has not been sequenced. Hence, its genetic and genomic studies have been limited. We report the first draft genome sequence of the silver pomfret obtained using a Next Generation Sequencing (NGS) technology. We assembled 38.7 Gb of nucleotides into scaffolds of 350 Mb with N50 of about 1.5 kb, using high quality paired end reads. These scaffolds represent 63.7% of the estimated silver pomfret genome length. The newly sequenced and assembled genome has 11.06% repetitive DNA regions, and this percentage is comparable to that of the tilapia genome. The genome analysis predicted 16 322 genes. About 91% of these genes showed homology with known proteins. Many gene clusters were annotated to protein and fatty-acid metabolism pathways that may be important in the context of the meat texture and immune system developmental processes. The reference genome can pave the way for the identification of many other genomic features that could improve breeding and population-management strategies, and it can also help characterize the genetic diversity of P. argenteus.
authors.affiliation: Environment and Life Sciences Research Center, Kuwait Institute for Scientific Research, Kuwait.|Environment and Life Sciences Research Center, Kuwait Institute for Scientific Research, Kuwait.
authors.forename: Sabah
authors.initials: S
authors.lastname: AlMomin
authors.affiliation: Environment and Life Sciences Research Center, Kuwait Institute for Scientific Research, Kuwait.|Environment and Life Sciences Research Center, Kuwait Institute for Scientific Research, Kuwait.
authors.forename: Vinod
authors.initials: V
authors.lastname: Kumar
authors.affiliation: Environment and Life Sciences Research Center, Kuwait Institute for Scientific Research, Kuwait.|Environment and Life Sciences Research Center, Kuwait Institute for Scientific Research, Kuwait.
authors.forename: Sami
authors.initials: S
authors.lastname: Al-Amad
authors.affiliation: Environment and Life Sciences Research Center, Kuwait Institute for Scientific Research, Kuwait.|Environment and Life Sciences Research Center, Kuwait Institute for Scientific Research, Kuwait.
authors.forename: Mohsen
authors.initials: M
authors.lastname: Al-Hussaini
authors.affiliation: Environment and Life Sciences Research Center, Kuwait Institute for Scientific Research, Kuwait.|Environment and Life Sciences Research Center, Kuwait Institute for Scientific Research, Kuwait.
authors.forename: Talal
authors.initials: T
authors.lastname: Dashti
authors.affiliation: Environment and Life Sciences Research Center, Kuwait Institute for Scientific Research, Kuwait.|Environment and Life Sciences Research Center, Kuwait Institute for Scientific Research, Kuwait.
authors.forename: Khaznah
authors.initials: K
authors.lastname: Al-Enezi
authors.affiliation: Environment and Life Sciences Research Center, Kuwait Institute for Scientific Research, Kuwait.|Environment and Life Sciences Research Center, Kuwait Institute for Scientific Research, Kuwait.
authors.forename: Abrar
authors.initials: A
authors.lastname: Akbar
mesh_terms: D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D016678:Genome; D059014:High-Throughput Nucleotide Sequencing; D010473:Perciformes / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017210:Tilapia / Q000235:genetics
keywords: Next Generation Sequencing; Pampus argenteus; Zobaidy; annotation de génome; assemblage de génome; assemblage de novo; de novo assembly; genome annotation; genome assembly; genome structure; silver pomfret; séquençage de nouvelle génération