title: Yeast Hosts for the Production of Recombinant Laccases: A Review.
abstract: Laccases are multi-copper oxidoreductases which catalyze the oxidation of a wide range of substrates during the simultaneous reduction of oxygen to water. These enzymes, originally found in fungi, plants, and other natural sources, have many industrial and biotechnological applications. They are used in the food, textile, pulp, and paper industries, as well as for bioremediation purposes. Although natural hosts can provide relatively high levels of active laccases after production optimization, heterologous expression can bring, moreover, engineered enzymes with desired properties, such as different substrate specificity or improved stability. Hence, diverse hosts suitable for laccase production are reviewed here, while the greatest emphasis is placed on yeasts which are commonly used for industrial production of various proteins. Different approaches to optimize the laccase expression and activity are also discussed in detail here.
authors.affiliation: Department of Membrane Transport, Institute of Physiology, Czech Academy of Sciences (CAS), Vídeňská 1083, 142 20, Prague 4, Czech Republic. antosova@biomed.cas.cz.
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Antošová
authors.affiliation: Department of Membrane Transport, Institute of Physiology, Czech Academy of Sciences (CAS), Vídeňská 1083, 142 20, Prague 4, Czech Republic. hana.sychrova@fgu.cas.cz.
authors.forename: Hana
authors.initials: H
authors.lastname: Sychrová
mesh_terms: D000818:Animals; D001419:Bacteria / Q000235:genetics; D005658:Fungi / Q000235:genetics; D007313:Insecta / Q000166:cytology / Q000235:genetics; D042845:Laccase / Q000235:genetics* / Q000378:metabolism; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000235:genetics* / Q000378:metabolism; D013379:Substrate Specificity; D015003:Yeasts / Q000235:genetics*
chemical_list: D011994:Recombinant Proteins; D042845:Laccase
keywords: Expression optimization; Heterologous expression; Laccase; Recombinant; Yeasts