title: Homocysteine Levels Are not Related to Telomere Length in Cord Blood Leukocytes of Newborns.
abstract: Objective Elevated homocysteine (HC) levels and/or shortened telomere length (TL) are associated with adverse medical conditions. Our objective is to investigate the relationship between HC and TL in cord blood leukocytes of newborns. Study Design This is a nested study from a prospective cohort from 2011 to 2012 in pregnant women admitted for delivery at a university-affiliated hospital. Cord blood was collected at delivery and genomic DNA was analyzed using quantitative PCR. The telomere-to-single copy gene ratio method was employed to quantify TL. Newborn HC levels were measured. generalized linear regression modeling (GLM) and bootstrap statistical analyses were performed. Results Seventy-seven maternal-fetal dyads with a mean gestational age of 39 weeks were included. The distribution of the coefficient of homocysteine showed most values greater than zero demonstrating that homocysteine had a positive relationship with TL. In 915 of 10,000 (9.15%) iterations, the p-value was < 0.05 demonstrating a positive effect. Conclusion Increasing newborn concentrations of HC are not associated with decreasing TL. Larger, prospective studies are needed to confirm these findings and long-term implications.
authors.affiliation: Department of Obstetrics and Gynecology, University of South Florida Morsani College of Medicine, Tampa, Florida.
authors.forename: Rachel G
authors.initials: RG
authors.lastname: Sinkey
authors.affiliation: Department of Family and Community Medicine, Baylor College of Medicine, Houston, Texas.
authors.forename: Hamisu M
authors.initials: HM
authors.lastname: Salihu
authors.affiliation: Department of Epidemiology and Biostatistics, College of Public Health, University of South Florida, Tampa, Florida.
authors.forename: Lindsey M
authors.initials: LM
authors.lastname: King
authors.affiliation: Department of Epidemiology and Biostatistics, College of Public Health, University of South Florida, Tampa, Florida.
authors.forename: Arnut
authors.initials: A
authors.lastname: Paothong
authors.affiliation: Department of Obstetrics and Gynecology, University of South Florida Morsani College of Medicine, Tampa, Florida.
authors.forename: Adetola
authors.initials: A
authors.lastname: Louis-Jacques
authors.affiliation: Department of Global Health, College of Public Health, University of South Florida, Tampa, Florida.
authors.forename: Anupam
authors.initials: A
authors.lastname: Pradhan
authors.affiliation: Department of Obstetrics and Gynecology, University of South Florida Morsani College of Medicine, Tampa, Florida.
authors.forename: Karen L
authors.initials: KL
authors.lastname: Bruder
authors.affiliation: Department of Family and Community Medicine, Baylor College of Medicine, Houston, Texas.
authors.forename: Roger
authors.initials: R
authors.lastname: Zoorob
authors.affiliation: Department of Cancer Epidemiology, H. Lee Moffitt Cancer Center and Research Institute, Tampa, Florida.
authors.forename: Erin
authors.initials: E
authors.lastname: Siegel
authors.affiliation: Department of Cancer Epidemiology, H. Lee Moffitt Cancer Center and Research Institute, Tampa, Florida.
authors.forename: Bridget
authors.initials: B
authors.lastname: Riggs
authors.affiliation: Department of Obstetrics and Gynecology, University of South Florida Morsani College of Medicine, Tampa, Florida.
authors.forename: Valerie E
authors.initials: VE
authors.lastname: Whiteman
mesh_terms: D000293:Adolescent; D000328:Adult; D004247:DNA / Q000032:analysis*; D005260:Female; D005312:Fetal Blood / Q000166:cytology; D005431:Florida; D005865:Gestational Age; D006710:Homocysteine / Q000097:blood*; D006801:Humans; D007231:Infant, Newborn; D007962:Leukocytes / Q000502:physiology*; D016014:Linear Models; D011247:Pregnancy; D011446:Prospective Studies; D013223:Statistics as Topic; D016615:Telomere / Q000378:metabolism / Q000648:ultrastructure*; D055815:Young Adult
chemical_list: D006710:Homocysteine; D004247:DNA