title: Cellular peptidyl-prolyl cis/trans isomerase Pin1 facilitates replication of feline coronavirus.
abstract: Although feline coronavirus (FCoV) causes feline infectious peritonitis (FIP), which is a fatal infectious disease, there are no effective therapeutic medicines or vaccines. Previously, in vitro studies have shown that cyclosporin (CsA) and FK506 inhibit virus replication in diverse coronaviruses. CsA and FK506 are targets of clinically relevant immunosuppressive drugs and bind to cellular cyclophilins (Cyps) or FK506 binding proteins (FKBPs), respectively. Both Cyp and FKBP have peptidyl-prolyl cis-trans isomerase (PPIase) activity. However, protein interacting with NIMA (Pin1), a member of the parvulin subfamily of PPIases that differs from Cyps and FKBPs, is essential for various signaling pathways. Here we demonstrated that genetic silencing or knockout of Pin1 resulted in decreased FCoV replication in vitro. Dipentamethylene thiuram monosulfide, a specific inhibitor of Pin1, inhibited FCoV replication. These data indicate that Pin1 modulates FCoV propagation.
authors.affiliation: Department of Veterinary Hygiene, Veterinary School, Nippon Veterinary and Life Science University, 1-7-1 Kyounan, Musashino, Tokyo 180-8602, Japan. Electronic address: ytanaka@nvlu.ac.jp.
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Tanaka
authors.affiliation: Department of Veterinary Hygiene, Veterinary School, Nippon Veterinary and Life Science University, 1-7-1 Kyounan, Musashino, Tokyo 180-8602, Japan.
authors.forename: Arisa
authors.initials: A
authors.lastname: Amano
authors.affiliation: Department of Veterinary Hygiene, Veterinary School, Nippon Veterinary and Life Science University, 1-7-1 Kyounan, Musashino, Tokyo 180-8602, Japan.
authors.forename: Masateru
authors.initials: M
authors.lastname: Morisaki
authors.affiliation: Department of Microbiology and Immunology, Division of Molecular Virology, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan.
authors.forename: Yuka
authors.initials: Y
authors.lastname: Sato
authors.affiliation: Department of Bacteriology, Faculty of Medicine, Juntendo University, Tokyo 113-8421, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Sasaki
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002415:Cats; D002460:Cell Line; D016765:Coronavirus, Feline / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000502:physiology; D021983:Cyclophilins / Q000187:drug effects; D016572:Cyclosporine / Q000494:pharmacology; D004261:DNA Replication / Q000187:drug effects; D055808:Drug Discovery; D016766:Feline Infectious Peritonitis / Q000821:virology; D055786:Gene Knockout Techniques; D007166:Immunosuppressive Agents / Q000494:pharmacology; D000072340:NIMA-Interacting Peptidylprolyl Isomerase / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D010880:Piperidines / Q000494:pharmacology; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D022021:Tacrolimus Binding Proteins / Q000494:pharmacology; D013893:Thiram / Q000031:analogs & derivatives / Q000494:pharmacology; D014779:Virus Replication / Q000187:drug effects / Q000502:physiology*
chemical_list: D007166:Immunosuppressive Agents; D000072340:NIMA-Interacting Peptidylprolyl Isomerase; D010880:Piperidines; D034741:RNA, Small Interfering; C541134:dipentamethylene thiuram monosulfide; D013893:Thiram; D016572:Cyclosporine; D021983:Cyclophilins; D022021:Tacrolimus Binding Proteins
keywords: Dipentamethylene thiuram monosulfide (DTM); Feline coronavirus; Peptidyl-prolyl cis-trans isomerase; Pin1