title: Mouse embryonic fibroblasts exhibit extensive developmental and phenotypic diversity.
abstract: Analysis of embryonic fibroblasts from GFP reporter mice indicates that the fibroblast cell type harbors a large collection of developmentally and phenotypically heterogeneous subtypes. Some of these cells exhibit multipotency, whereas others do not. Multiparameter flow cytometry analysis shows that a large number of distinct populations of fibroblast-like cells can be found in cultures initiated from different embryonic organs, and cells sorted according to their surface phenotype typically retain their characteristics on continued propagation in culture. Similarly, surface phenotypes of individual cloned fibroblast-like cells exhibit significant variation. The fibroblast cell class appears to contain a very large number of denumerable subtypes.
authors.affiliation: Center for Computational and Integrative Biology, Massachusetts General Hospital, Boston, MA 02114; Department of Genetics, Harvard Medical School, Boston, MA 02115;
authors.forename: Prabhat K
authors.initials: PK
authors.lastname: Singhal
authors.affiliation: Center for Computational and Integrative Biology, Massachusetts General Hospital, Boston, MA 02114; Department of Orthopedics, Harvard Medical School, Boston, MA 02115;
authors.forename: Slim
authors.initials: S
authors.lastname: Sassi
authors.affiliation: Center for Computational and Integrative Biology, Massachusetts General Hospital, Boston, MA 02114; Department of Genetics, Harvard Medical School, Boston, MA 02115;
authors.forename: Lan
authors.initials: L
authors.lastname: Lan
authors.affiliation: Steele Laboratory of Tumor Biology, Department of Radiation Oncology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114.
authors.forename: Patrick
authors.initials: P
authors.lastname: Au
authors.affiliation: Center for Computational and Integrative Biology, Massachusetts General Hospital, Boston, MA 02114;
authors.forename: Stefan C
authors.initials: SC
authors.lastname: Halvorsen
authors.affiliation: Steele Laboratory of Tumor Biology, Department of Radiation Oncology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114.
authors.forename: Dai
authors.initials: D
authors.lastname: Fukumura
authors.affiliation: Steele Laboratory of Tumor Biology, Department of Radiation Oncology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114 jain@steele.mgh.harvard.edu bseed@ccib.mgh.harvard.edu.
authors.forename: Rakesh K
authors.initials: RK
authors.lastname: Jain
authors.affiliation: Center for Computational and Integrative Biology, Massachusetts General Hospital, Boston, MA 02114; Department of Genetics, Harvard Medical School, Boston, MA 02115; jain@steele.mgh.harvard.edu bseed@ccib.mgh.harvard.edu.
authors.forename: Brian
authors.initials: B
authors.lastname: Seed
mesh_terms: D000818:Animals; D002460:Cell Line; D002469:Cell Separation; D004622:Embryo, Mammalian / Q000166:cytology*; D005347:Fibroblasts / Q000378:metabolism / Q000502:physiology*; D005434:Flow Cytometry; D049452:Green Fluorescent Proteins / Q000235:genetics; D051379:Mice; D008822:Mice, Transgenic; D020286:Pericytes / Q000166:cytology / Q000502:physiology; D010641:Phenotype; D059010:Single-Cell Analysis; D059467:Transcriptome; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics
chemical_list: D042461:Vascular Endothelial Growth Factor A; D049452:Green Fluorescent Proteins
keywords: embryo; fibroblast; heterogeneity; lineage; phenotype