title: PON-1 Activity and Plasma 8-Isoprostane Concentration in Patients with Angiographically Proven Coronary Artery Disease.
abstract: The aim of the study was to estimate association of the extent of angiographically proven coronary artery disease (CAD) with plasma 8-isoprostane F2 (8-iso-PGF2α) levels as a reliable marker of lipid peroxidation and serum activity of paraoxonase-1, which demonstrates the ability to protect against lipid oxidation. The study included 105 patients with angiographically documented CAD (CAD+) and 45 patients with negative results of coronary angiography (CAD-). Compared to the control group CAD+ patients were characterized by increased 8-iso-PGF2α levels (P = 0.007) and reduced activity of PON-1 towards paraoxon (PONase, P = 0.002) and phenyl acetate (AREase, P = 0.037). Univariate correlation analysis indicated that 8-iso-PGF2α concentrations were positively associated with the severity of CAD as evaluated by the Gensini score (R = 0.41, P < 0.001) while PONase activity (R = -0.26, P < 0.05) and AREase activity (R = -0.23, P < 0.05) were inversely correlated with CAD severity. PONase activity and 8-iso-PGF2α concentration remained independent determinant of atherosclerosis severity in multiple linear regression after adjusting for age, gender, smoking habits, hypertension, type 2 diabetes, statin therapy, and HDL-C and TAG concentration (β coefficients -0.267; P < 0.05 and 0.368; P < 0.001, resp.). The results suggest that PON-1 activity and 8-iso-PGF2α concentration are associated with the presence and extent of coronary stenosis and may be considered additional markers of coronary artery disease.
authors.affiliation: Department of Clinical Chemistry, Medical University of Gdańsk, Marii Skłodowskiej-Curie 3a, 80-210 Gdansk, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Kuchta
authors.affiliation: Chair and Clinic of Internal Medicine, Connective Tissue Diseases and Geriatrics, Medical University of Gdańsk, Marii Skłodowskiej-Curie 3a, 80-210 Gdansk, Poland.
authors.forename: Adrian
authors.initials: A
authors.lastname: Strzelecki
authors.affiliation: Department of Clinical Chemistry, Medical University of Gdańsk, Marii Skłodowskiej-Curie 3a, 80-210 Gdansk, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Ćwiklińska
authors.affiliation: Department of Clinical Chemistry, Medical University of Gdańsk, Marii Skłodowskiej-Curie 3a, 80-210 Gdansk, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Totoń
authors.affiliation: First Chair & Clinic of Cardiology, Medical University of Gdańsk, Marii Skłodowskiej-Curie 3a, 80-210 Gdansk, Poland.
authors.forename: Marcin
authors.initials: M
authors.lastname: Gruchała
authors.affiliation: Chair and Clinic of Internal Medicine, Connective Tissue Diseases and Geriatrics, Medical University of Gdańsk, Marii Skłodowskiej-Curie 3a, 80-210 Gdansk, Poland.
authors.forename: Zbigniew
authors.initials: Z
authors.lastname: Zdrojewski
authors.affiliation: Department of Clinical Chemistry, Medical University of Gdańsk, Marii Skłodowskiej-Curie 3a, 80-210 Gdansk, Poland.
authors.forename: Barbara
authors.initials: B
authors.lastname: Kortas-Stempak
authors.affiliation: Department of Clinical Chemistry, Medical University of Gdańsk, Marii Skłodowskiej-Curie 3a, 80-210 Gdansk, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Gliwińska
authors.affiliation: Department of Clinical Chemistry, Medical University of Gdańsk, Marii Skłodowskiej-Curie 3a, 80-210 Gdansk, Poland.
authors.forename: Kamil
authors.initials: K
authors.lastname: Dąbkowski
authors.affiliation: Department of Clinical Chemistry, Medical University of Gdańsk, Marii Skłodowskiej-Curie 3a, 80-210 Gdansk, Poland.
authors.forename: Maciej
authors.initials: M
authors.lastname: Jankowski
mesh_terms: D000368:Aged; D043303:Aryldialkylphosphatase / Q000378:metabolism*; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism; D008076:Cholesterol, HDL / Q000097:blood; D017023:Coronary Angiography; D003324:Coronary Artery Disease / Q000000981:diagnostic imaging / Q000378:metabolism / Q000473:pathology*; D015237:Dinoprost / Q000031:analogs & derivatives* / Q000097:blood / Q000737:chemistry / Q000378:metabolism; D005260:Female; D006801:Humans; D016014:Linear Models; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D012720:Severity of Illness Index
chemical_list: D008076:Cholesterol, HDL; C075750:8-epi-prostaglandin F2alpha; D015237:Dinoprost; D002265:Carboxylic Ester Hydrolases; C019734:arylesterase; D043303:Aryldialkylphosphatase; C468406:PON1 protein, human