title: Liver transcriptome sequencing and de novo annotation of the large yellow croaker (Larimichthy crocea) under heat and cold stress.
abstract: Large yellow croaker is an economically important marine fish in China. To understand the molecular mechanisms of this fish under temperature stress, liver transcriptomes of large yellow croakers undergoing heat and cold stress were investigated. The results showed that 130,246 contigs from cold stressed fish (CS), 109,203 contigs from control fish (NS) and 98,569 contigs from heat stressed fish (HS) were obtained from the liver transcriptomes with de novo analysis, respectively. And in total, 9467 (6113 up-regulated and 3364 down-regulated), 5350 (2185 up-regulated and 3165 down-regulated), 10,622 (3146 up-regulated and 7477 down-regulated) significantly differentially expressed genes were identified in CS-NS, HS-NS, and HS-CS, respectively. Pathway enrichment analysis showed that many pathways including those of energy metabolism and metabolic pathways were affected after temperature stress. Further qPCR analysis also confirmed that the expression levels of genes coding for key enzymes in metabolic pathways were dramatically changed.
authors.affiliation: College of Marine Sciences, Ningbo University, Ningbo, Zhejiang 315211, PR China; School of Life Science, Taizhou University, Taizhou, Zhejiang 317000, PR China. Electronic address: wutongye1979@126.com.
authors.forename: Baoying
authors.initials: B
authors.lastname: Qian
authors.affiliation: College of Marine Sciences, Ningbo University, Ningbo, Zhejiang 315211, PR China; Collaborative Innovation Center for Zhejiang Marine High-Efficiency and Healthy Aquaculture, Ningbo University, Ningbo, Zhejiang, PR China. Electronic address: xueliangyi@nbu.edu.cn.
authors.forename: Liangyi
authors.initials: L
authors.lastname: Xue
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D050260:Carbohydrate Metabolism / Q000235:genetics / Q000502:physiology; D002952:Citric Acid Cycle; D058639:Cold-Shock Response / Q000502:physiology*; D004041:Dietary Fats / Q000378:metabolism; D005399:Fishes / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000502:physiology*; D005943:Gluconeogenesis; D005947:Glucose / Q000378:metabolism; D018869:Heat-Shock Response / Q000502:physiology*; D008099:Liver / Q000378:metabolism*; D010085:Oxidative Phosphorylation; D012313:RNA / Q000235:genetics / Q000378:metabolism; D059467:Transcriptome
chemical_list: D000596:Amino Acids; D004041:Dietary Fats; D012313:RNA; D005947:Glucose
keywords: Cold stress; Heat stress; Large yellow croaker; Liver transcriptome