title: Membranicola marinusgen. nov., sp. nov., a new member of the familySaprospiraceaeisolated from a biofilter in a recirculating aquaculture system.
abstract: A Gram-staining-negative bacterial strain (termed CZ-AZ5T) was isolated from a biological filter in a marine recirculating aquaculture system in Tianjin, China. Its taxonomic status was determined using a polyphasic approach. CZ-AZ5Tcells were non-spore-forming, non-motile rods, 0.6-0.7 μm wide and 3.0-3.7 μm long. CZ-AZ5Twas strictly heterotrophic, aerobic, oxidase-negative and catalase-positive. Growth occurred in the temperature range 20-40 °C (optimal: 30 °C), pH range 6.0-8.5 (optimal: pH 7.5) and salinity range 0-5 % (w/v) NaCl (optimal: 1 %). In phylogenetic analyses based on 16S rRNA gene sequences, CZ-AZ5Twas assigned to the familySaprospiraceae(phylumBacteroidetes) and was clustered with the generaSaprospiraandAureispirawithin this family. It showed highest sequence similarity to 'CandidatusHaliscomenobacter calcifugiens' (86.2 %), followed bySaprospira grandisATCC 23119T(85.7 %) andLewinella persicaT-3T(85.6 %). DNA G+C content was 40.1 mol%, the major menaquinone was MK-7, and the major cellular fatty acids (>10 %) were C16 : 1ω7cand iso-C15 : 0. Our phenotypic, chemotaxonomic and phylogenetic observations, taken together, led us to conclude that strain CZ-AZ5Trepresents a novel species and genus of the familySaprospiraceae, for which the nameMembranicola marinusgen. nov., sp. nov. is proposed. The type strain is CZ-AZ5T( = CGMCC 1.13179T= JCM 18886T).
authors.affiliation: R&D Center of Marine Biotechnology, Institute of Oceanology, Chinese Academy of Sciences,Qingdao 266071,PR China.
authors.forename: Xian
authors.initials: X
authors.lastname: Li
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences,Beijing 100101,PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.affiliation: R&D Center of Marine Biotechnology, Institute of Oceanology, Chinese Academy of Sciences,Qingdao 266071,PR China.
authors.forename: Zhu
authors.initials: Z
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences,Beijing 100101,PR China.
authors.forename: Liang-Zi
authors.initials: LZ
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences,Beijing 100101,PR China.
authors.forename: Zhi-Pei
authors.initials: ZP
authors.lastname: Liu
authors.affiliation: Nantong R&D Center of Marine Science and Technology, Institute of Oceanology, Chinese Academy of Sciences,Nantong 226019,PR China.|R&D Center of Marine Biotechnology, Institute of Oceanology, Chinese Academy of Sciences,Qingdao 266071,PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu