title: Molecular detection of Trypanosoma sp. and Blastocrithidia sp. (Trypanosomatidae) in phlebotomine sand flies (Psychodidae) in the Federal District of Brazil.
abstract: BACKGROUND
  This study describes the occurrence of trypanosomatids in phlebotomines in Brasília, Brazil.
  
  
  METHODS
  Two hundred and ten females of 13 sand fly species were analyzed by polymerase chain reaction (PCR) using different molecular markers (D7 24Sα rRNA, kDNA, and ITS1) and sequencing.
  
  
  RESULTS
  PCR revealed trypanosomatid-positive samples from Nyssomyia whitmani and Evandromyia evandroi, which were negative by kDNA and ITS1 Leishmania-specific PCRs. DNA sequence analysis of D7 24Sα rRNA amplicons indicated the occurrence of Blastocrithidia sp. and Trypanosoma sp. in Nyssomyia whitmani and Evandromyia evandroi, respectively.
  
  
  CONCLUSIONS
  Two trypanosomatid species other than Leishmania sp. were found to circulate in sand flies in Central Brazil.
authors.affiliation: Laboratório de Parasitologia Médica e Biologia de Vetores, Faculdade de Medicina, Universidade de Brasília, Brasília, Distrito Federal, Brazil.
authors.forename: Tauana de Sousa
authors.initials: Tde S
authors.lastname: Ferreira
authors.affiliation: Laboratório de Parasitologia Médica e Biologia de Vetores, Faculdade de Medicina, Universidade de Brasília, Brasília, Distrito Federal, Brazil.
authors.forename: Thaís Tâmara Castro
authors.initials: TT
authors.lastname: Minuzzi-Souza
authors.affiliation: Laboratório de Parasitologia Médica e Biologia de Vetores, Faculdade de Medicina, Universidade de Brasília, Brasília, Distrito Federal, Brazil.
authors.forename: Andrey José de
authors.initials: AJ
authors.lastname: Andrade
authors.affiliation: Laboratório de Parasitologia Médica e Biologia de Vetores, Faculdade de Medicina, Universidade de Brasília, Brasília, Distrito Federal, Brazil.
authors.forename: Thais Oliveira
authors.initials: TO
authors.lastname: Coelho
authors.affiliation: Laboratório de Parasitologia Médica e Biologia de Vetores, Faculdade de Medicina, Universidade de Brasília, Brasília, Distrito Federal, Brazil.
authors.forename: Douglas de Almeida
authors.initials: Dde A
authors.lastname: Rocha
authors.affiliation: Faculdade de Ceilândia, Universidade de Brasília, Ceilândia, Distrito Federal, Brazil.
authors.forename: Marcos Takashi
authors.initials: MT
authors.lastname: Obara
authors.affiliation: Laboratório Interdisciplinar de Biociências, Faculdade de Medicina, Universidade de Brasília, Brasília, Distrito Federal, Brazil.
authors.forename: Mariana
authors.initials: M
authors.lastname: Hecht
authors.affiliation: Laboratório Interdisciplinar de Biociências, Faculdade de Medicina, Universidade de Brasília, Brasília, Distrito Federal, Brazil.
authors.forename: Nadjar
authors.initials: N
authors.lastname: Nitz
authors.affiliation: Laboratório de Parasitologia Médica e Biologia de Vetores, Faculdade de Medicina, Universidade de Brasília, Brasília, Distrito Federal, Brazil.
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Gurgel-Gonçalves
mesh_terms: D000818:Animals; D001938:Brazil; D018105:DNA, Kinetoplast / Q000235:genetics*; D016054:DNA, Protozoan / Q000235:genetics*; D005260:Female; D007303:Insect Vectors / Q000145:classification / Q000469:parasitology*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D011576:Psychodidae / Q000145:classification / Q000469:parasitology*; D014351:Trypanosomatina / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D018105:DNA, Kinetoplast; D016054:DNA, Protozoan