title: Molecular screening for Rickettsia, Anaplasmataceae and Coxiella burnetii in Rhipicephalus sanguineus ticks from Malaysia.
abstract: A total of 44 Rhipicephalus sanguineus ticks collected from 23 dogs from Malaysia were screened for Rickettsia, Anaplasmataceae and Coxiella burnetii. Coxiella burnetii was detected in 59% (26/44) of ticks however Rickettsia and Anaplasmataceae were not detected in any of the ticks. In order to genotype the strains of C. burnetii, multispacer sequence typing (MST) was carried out using three different spacers. One of the spacers; Cox2 successfully amplified a fragment for which the full length sequence of 397 bp was obtained. The sequenced product revealed only a single nucleotide difference with the Cox2.3 type sequence.
authors.affiliation: Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
authors.forename: M
authors.initials: M
authors.lastname: Watanabe
authors.affiliation: Division of Education and Collaboration, Research Center for Zoonosis Control, Hokkaido University, Japan.
authors.forename: R
authors.initials: R
authors.lastname: Nakao
authors.affiliation: Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
authors.forename: S M
authors.initials: SM
authors.lastname: Amin-Babjee
authors.affiliation: Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
authors.forename: A M
authors.initials: AM
authors.lastname: Maizatul
authors.affiliation: Division of Education and Collaboration, Research Center for Zoonosis Control, Hokkaido University, Japan.
authors.forename: J H
authors.initials: JH
authors.lastname: Youn
authors.affiliation: Division of Education and Collaboration, Research Center for Zoonosis Control, Hokkaido University, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Qiu
authors.affiliation: Division of Education and Collaboration, Research Center for Zoonosis Control, Hokkaido University, Japan.
authors.forename: C
authors.initials: C
authors.lastname: Sugimoto
authors.affiliation: Department of Companion Animal Medicine and Surgery, Faculty of Veterinary Medicine, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
authors.forename: M
authors.initials: M
authors.lastname: Watanabe
mesh_terms: D000710:Anaplasmataceae / Q000302:isolation & purification*; D000818:Animals; D016997:Coxiella burnetii / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D004283:Dog Diseases / Q000469:parasitology; D004285:Dogs; D005260:Female; D005838:Genotype; D008296:Malaysia; D008297:Male; D008403:Mass Screening; D058889:Molecular Typing; D048508:Rhipicephalus sanguineus / Q000382:microbiology*; D012281:Rickettsia / Q000302:isolation & purification*; D017422:Sequence Analysis, DNA; D013984:Tick Infestations / Q000469:parasitology