title: DNA-Binding Motif of the Imprinted Transcription Factor PEG3.
abstract: Peg3 is an imprinted gene that is predicted to encode a DNA-binding zinc finger protein. This was previously demonstrated through Chromatin ImmunoPrecipitation-based Sequencing experiments. In the current study, we reanalyzed the previous ChIP-Seq results and further characterized the DNA-binding motif of PEG3. According to the results, PEG3 binds to the promoters and enhancers of a subset of genes that are closely associated with the known functions of Peg3. Some of these identified targets include Tufm, Mrpl45, Cry2, Per1, Slc25a29 and Slc38a2. With this set of targets, we derived a DNA-binding motif of PEG3, 5'-GTGGCAGT-3', which also provides a tabulated matrix that can be used for predicting other unknown genomic targets. Among the newly identified targets, we analyzed in detail the two loci, Slc38a2 and Slc38a4, which are known to be involved in neutral amino acid transport. The results indicated that PEG3 likely functions as a transcriptional repressor for these two loci. Overall, the current study provides a set of genomic targets and also redefines the DNA-binding motif for the imprinted transcription factor PEG3.
authors.affiliation: Department of Biological Sciences, Louisiana State University, Baton Rouge, Louisiana, 70803, United States of America.
authors.forename: Suman
authors.initials: S
authors.lastname: Lee
authors.affiliation: Department of Biological Sciences, Louisiana State University, Baton Rouge, Louisiana, 70803, United States of America.
authors.forename: An
authors.initials: A
authors.lastname: Ye
authors.affiliation: Department of Biological Sciences, Louisiana State University, Baton Rouge, Louisiana, 70803, United States of America.
authors.forename: Joomyeong
authors.initials: J
authors.lastname: Kim
mesh_terms: D026924:Amino Acid Transport System A / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001665:Binding Sites; D047369:Chromatin Immunoprecipitation; D056931:Cryptochromes / Q000235:genetics; D004247:DNA / Q000378:metabolism*; D018392:Genomic Imprinting; D051741:Kruppel-Like Transcription Factors / Q000235:genetics / Q000378:metabolism*; D008810:Mice, Inbred C57BL; D024101:Mitochondrial Proteins / Q000235:genetics; D010444:Peptide Elongation Factor Tu / Q000235:genetics; D056950:Period Circadian Proteins / Q000235:genetics; D014157:Transcription Factors
chemical_list: D026924:Amino Acid Transport System A; C538967:Cry2 protein, mouse; D056931:Cryptochromes; D051741:Kruppel-Like Transcription Factors; D024101:Mitochondrial Proteins; C097901:Peg3 protein, mouse; C539023:Per1 protein, mouse; D056950:Period Circadian Proteins; C498798:Slc38a2 protein, mouse; C420522:Slc38a4 protein, mouse; D014157:Transcription Factors; D004247:DNA; D010444:Peptide Elongation Factor Tu; C557064:Tufm protein, mouse