title: The role of periodontal ASIC3 in orofacial pain induced by experimental tooth movement in rats.
abstract: This study aimed to clarify the roles of Acid-sensing ion channel 3 (ASIC3) in orofacial pain following experimental tooth movement.
  
  
  
  Sixty male Sprague-Dawley rats were divided into the experimental group (40g, n = 30) and the sham group (0g, n = 30). Closed coil springs were ligated between maxillary incisor and molars to achieve experimental tooth movement. Rat grimace scale (RGS) scores were assessed at 0, 1, 3, 5, 7, and 14 days after the placement of the springs. ASIC3 immunostaining was performed and the expression levels of ASIC3 were measured through integrated optical density/area in Image-Pro Plus 6.0. Moreover, 18 rats were divided into APETx2 group (n = 6), amiloride group (n = 6), and vehicle group (n = 6), and RGS scores were obtained compared among them to verify the roles of ASIC3 in orofacial pain following tooth movement.
  
  
  
  ASIC3 expression levels became significantly higher in the experimental group than in sham group on 1, 3, and 5 days and became similar on 7 and 14 days. Pain levels (RGS scores) increased in both groups and were significantly higher in the experimental group on 1, 3, 5, and 7 days and were similar on 14 days. Periodontal ASIC3 expression levels were correlated with orofacial pain levels following experimental tooth movement. Periodontal administrations of ASIC3 antagonists (APETx2 and amiloride) could alleviate pain.
  
  
  
  This study needs to be better evidenced by RNA interference of ASIC3 in periodontal tissues in rats following experimental tooth movement. Moreover, we hope further studies would concentrate on the pain perception of ASIC3 knockout (ASIC3-/-) mice.
  
  
  
  Our results suggest that periodontal ASIC3 plays an important role in orofacial pain induced by experimental tooth movement.
authors.affiliation: *Department of Orthodontics, State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu.
authors.forename: Meiya
authors.initials: M
authors.lastname: Gao
authors.affiliation: *Department of Orthodontics, State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu.
authors.forename: Hu
authors.initials: H
authors.lastname: Long
authors.affiliation: **Department of Orthodontics, North Sichuan Medical College, Nanchong, China.
authors.forename: Wenqiang
authors.initials: W
authors.lastname: Ma
authors.affiliation: *Department of Orthodontics, State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu.
authors.forename: Lina
authors.initials: L
authors.lastname: Liao
authors.affiliation: *Department of Orthodontics, State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu.
authors.forename: Xin
authors.initials: X
authors.lastname: Yang
authors.affiliation: *Department of Orthodontics, State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: *Department of Orthodontics, State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu.
authors.forename: Di
authors.initials: D
authors.lastname: Shan
authors.affiliation: *Department of Orthodontics, State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu.
authors.forename: Renhuan
authors.initials: R
authors.lastname: Huang
authors.affiliation: *Department of Orthodontics, State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu.
authors.forename: Fan
authors.initials: F
authors.lastname: Jian
authors.affiliation: *Department of Orthodontics, State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: *Department of Orthodontics, State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, wenlilai@scu.edu.cn.
authors.forename: Wenli
authors.initials: W
authors.lastname: Lai
mesh_terms: D062565:Acid Sensing Ion Channels / Q000378:metabolism / Q000502:physiology*; D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D005149:Facial Expression; D005157:Facial Pain / Q000209:etiology* / Q000378:metabolism; D008297:Male; D010147:Pain Measurement / Q000379:methods; D010519:Periodontium / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D014087:Tooth Movement Techniques / Q000009:adverse effects*
chemical_list: C568269:ASIC3 protein, rat; D062565:Acid Sensing Ion Channels; D000584:Amiloride