title: Hybrid graphene/Au activatable theranostic agent for multimodalities imaging guided enhanced photothermal therapy.
abstract: Photothermal therapy (PTT) has been increasingly investigated. However, there are still challenges in strategies that can further enhance photoconversion efficiency and improve photothermal tumor ablation effect of current nanomaterials. Herein, we developed a fluorescent/photoacoustic imaging guided PTT agent by seeding Gold (Au) nanoparticles onto graphene oxide (GO). Near infrared dye (Cy5.5) labeled-matrix metalloproteinase-14 (MMP-14) substrate (CP) was conjugated onto the GO/Au complex (GA) forming tumor targeted theranostic probe (CPGA), whereCy5.5 fluorescent signal is quenched by Surface Plasmon Resonance (SPR) capacity from both GO and Au, yet it can boost strong fluorescence signals after degradation by MMP-14. The photothermal effect of GA hybrid was found significantly elevated compared with Au or GO alone. After intravenous administration of CPGA into SCC7 tumor-bearing mice, high fluorescence and PA signals were observed in the tumor area over time, which peaked at the 6 h time point (tumor-to-normal tissue ratio of 3.64 ± 0.51 for optical imaging and 2.5 ± 0.27 for PA imaging). The tumors were then irradiated with a laser, and an excellent tumor inhibition was observedwithoutrecurrence. Our studies further encourage applications of the hybrid nanocomposite for image-guided enhanced PTT in biomedical applications, especially in cancer theranostics.
authors.affiliation: Department of Nuclear Medicine, China-Japan Union Hospital, Jilin University, Changchun, Jilin, 130033, China.
authors.forename: Shi
authors.initials: S
authors.lastname: Gao
authors.affiliation: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics & Center for Molecular Imaging and Translational Medicine, School of Public Health, Xiamen University, Xiamen, 361005, China.
authors.forename: Liwen
authors.initials: L
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics & Center for Molecular Imaging and Translational Medicine, School of Public Health, Xiamen University, Xiamen, 361005, China.
authors.forename: Guohao
authors.initials: G
authors.lastname: Wang
authors.affiliation: School of Radiation Medicine and Protection & School for Radiological and Interdisciplinary Sciences (RAD-X), Collaborative Innovation Center of Suzhou Nano Science and Technology & Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, Medical College of Soochow University, Suzhou, Jiangsu, 215123, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Yang
authors.affiliation: Department of Nuclear Medicine, China-Japan Union Hospital, Jilin University, Changchun, Jilin, 130033, China.
authors.forename: Minglong
authors.initials: M
authors.lastname: Chen
authors.affiliation: Department of Ophthalmology Second Hospital, Jilin University, Changchun, Jilin, 130033, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Tian
authors.affiliation: Department of Nuclear Medicine, China-Japan Union Hospital, Jilin University, Changchun, Jilin, 130033, China. Electronic address: maqingjiejlu@163.com.
authors.forename: Qingjie
authors.initials: Q
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics & Center for Molecular Imaging and Translational Medicine, School of Public Health, Xiamen University, Xiamen, 361005, China. Electronic address: lei.zhu@xmu.edu.cn.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhu
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D005260:Female; D006046:Gold / Q000627:therapeutic use; D006108:Graphite / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000627:therapeutic use*; D051379:Mice; D008819:Mice, Nude; D008856:Microscopy, Fluorescence / Q000379:methods; D064847:Multimodal Imaging / Q000379:methods*; D053769:Nanocapsules / Q000737:chemistry / Q000648:ultrastructure; D009374:Neoplasms, Experimental / Q000188:drug therapy* / Q000473:pathology*; D010778:Photochemotherapy / Q000379:methods*; D000068936:Theranostic Nanomedicine / Q000379:methods; D016896:Treatment Outcome
chemical_list: D053769:Nanocapsules; D006046:Gold; D006108:Graphite
keywords: Activatable probes; Graphene oxide; Image-guided therapy; Matrix metalloproteinase; Photothermal therapies