title: Feline mesenchymal stem cells and supernatant inhibit reactive oxygen species production in cultured feline neutrophils.
abstract: Feline bone marrow-derived MSCs (BMMSCs), adipose-derived MSCs (AMSCs) and fibroblasts (FBs) were isolated and cultured. Tri-lineage differentiation assays and flow cytometry were used to characterize MSCs. Neutrophils (NPs) were isolated from whole blood and the NPs production of reactive oxygen reactive oxygen species (ROS) was measured. NPs were cultured alone, with MSC culture supernatant (SN), BMMSCs or AMSCs. NPs incubated with BMMSCs had significantly lower ROS production than NPs incubated with AMSCs (p=0.0006) or FB (p<0.0001); NPs ROS production significantly decreased with increasing BMMSC cell number (p=0.0023) and significantly increased with NPs were incubated with FB compared to BMMSC (p=0.0003). Both BMMSC SN and AMSC SN had statistically significantly lower ROS production than FB SN when incubated with NPs (both p<0.0001). ROS production was significantly reduced with increased fractions of SN from BMMSCs (p=0.0467) and AMSCs (p=0.0017).
authors.affiliation: Department of Large Animal Medicine, College of Veterinary Medicine, University of Georgia, 2200 College Station Road, Athens, GA 30602, United States.
authors.forename: Jennifer L
authors.initials: JL
authors.lastname: Mumaw
authors.affiliation: Department of Small Animal Medicine and Surgery, College of Veterinary Medicine, University of Georgia, 2200 College Station Road, Athens, GA 30602, United States. Electronic address: cws@uga.edu.
authors.forename: Chad W
authors.initials: CW
authors.lastname: Schmiedt
authors.affiliation: Department of Large Animal Medicine, College of Veterinary Medicine, University of Georgia, 2200 College Station Road, Athens, GA 30602, United States.
authors.forename: Sarah
authors.initials: S
authors.lastname: Breidling
authors.affiliation: Department of Large Animal Medicine, College of Veterinary Medicine, University of Georgia, 2200 College Station Road, Athens, GA 30602, United States.
authors.forename: Alex
authors.initials: A
authors.lastname: Sigmund
authors.affiliation: Department of Large Animal Medicine, College of Veterinary Medicine, University of Georgia, 2200 College Station Road, Athens, GA 30602, United States.
authors.forename: Natalie A
authors.initials: NA
authors.lastname: Norton
authors.affiliation: Department of Large Animal Medicine, College of Veterinary Medicine, University of Georgia, 2200 College Station Road, Athens, GA 30602, United States.
authors.forename: Merrilee
authors.initials: M
authors.lastname: Thoreson
authors.affiliation: Department of Large Animal Medicine, College of Veterinary Medicine, University of Georgia, 2200 College Station Road, Athens, GA 30602, United States.
authors.forename: John F
authors.initials: JF
authors.lastname: Peroni
authors.affiliation: Department of Large Animal Medicine, College of Veterinary Medicine, University of Georgia, 2200 College Station Road, Athens, GA 30602, United States.
authors.forename: David J
authors.initials: DJ
authors.lastname: Hurley
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells; D002415:Cats; D002460:Cell Line; D005347:Fibroblasts / Q000378:metabolism*; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D009504:Neutrophils / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism*
chemical_list: D017382:Reactive Oxygen Species
keywords: Feline; Mesenchymal stem cell; Neutrophils; Reactive oxygen species