title: Detoxification and safety evaluation of aflatoxin B1 in peanut oil using alkali refining.
abstract: BACKGROUND
  Aflatoxin B1 (AFB1 ) is often detected in peanut oil, which comes from contaminated peanuts. AFB1 in peanut oil seriously threatens the health of consumers. However, there are few methods to effectively remove AFB1 in peanut oil. This study aimed to use an alkali-refining method to degrade AFB1 in peanut oil efficiently without increasing the equipment of oil and fat refining.
  
  
  RESULTS
  The optimum detoxifying conditions of AFB1 in peanut oil with alkali refining were established using response surface methodology (RSM), and the safety of peanut oil after being refined with alkali was evaluated based on the Ames tests and HepG2 cell viability. The results showed that AFB1 in peanut oil was decreased from 34.78 to 0.37 µg kg(-1) (98.94% reduction) under the optimum detoxifying conditions, i.e. when the initial temperature of alkali refining was 43.51 °C, the amount of excess alkali was 0.30%, the content of alkali solution was 23.42% and the end temperature of alkali refining was 77.07 °C. The acid value and color of peanut oil refined by alkali were improved significantly, while the peroxide value was increased within an acceptable level. The safety of peanut oil contaminated by AFB1 was improved significantly after being refined with alkali.
  
  
  CONCLUSIONS
  These results indicate that alkali refining is an effective method for removing AFB1 in peanut oil. The optimum detoxifying conditions of AFB1 in peanut oil with alkali refining could be used to guide the production of oil companies for ensuring food safety. © 2015 Society of Chemical Industry.
authors.affiliation: College of Food Science and Engineering, Shandong Agricultural University, No. 61, Daizong Street, Taian, 271018, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Ji
authors.affiliation: College of Food Science and Engineering, Shandong Agricultural University, No. 61, Daizong Street, Taian, 271018, China.
authors.forename: Enjie
authors.initials: E
authors.lastname: Diao
authors.affiliation: College of Food Science and Engineering, Shandong Agricultural University, No. 61, Daizong Street, Taian, 271018, China.
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Li
authors.affiliation: College of Food Science and Engineering, Shandong Agricultural University, No. 61, Daizong Street, Taian, 271018, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: College of Food Science and Engineering, Shandong Agricultural University, No. 61, Daizong Street, Taian, 271018, China.
authors.forename: Haizhou
authors.initials: H
authors.lastname: Dong
mesh_terms: D016604:Aflatoxin B1 / Q000737:chemistry* / Q000494:pharmacology / Q000633:toxicity; D010367:Arachis / Q000382:microbiology*; D002470:Cell Survival / Q000187:drug effects; D003666:Decontamination; D005511:Food Handling; D005516:Food Microbiology; D064088:Hazard Analysis and Critical Control Points; D056945:Hep G2 Cells / Q000187:drug effects; D006801:Humans
chemical_list: D016604:Aflatoxin B1
keywords: aflatoxin B1; alkali refining; detoxification; peanut oil; safety evaluation