title: Phylogenetic evidence for a new species of Barbus in the Danube River basin.
abstract: Three species of small-sized rheophilic Barbus fishes are endemic to and widely distributed throughout the mountain regions in the Danube River basin. In Hungary, barbels referred to as B. petenyi occur in streams in the foothills of the Carpathians near the borders with Slovakia, Ukraine and Romania. However, up to now, no genetic investigations were carried out on rheophilic barbels in this region. This study aims to clarify the taxonomic identity and distribution of the rheophilic barbels in the Hungarian plain based on molecular and morphological analyses. Two mitochondrial genes (cytochrome b, ATPase 6/8) and one nuclear gene (beta-actin intron 2) were sequenced and several morphometric and meristic characters were recorded. Phylogenetic and morphological analyses revealed that there are four genetically distinct lineages among the rheophilic barbels in the Carpathian Basin. The results demonstrated that North-Hungarian Barbus populations belong to B. carpathicus and that B. petenyi presumably does not occur in Hungary. As expected, B. balcanicus was only recorded in samples from the Balkans analyzed for reference. A distinct species, new to science, was discovered to be present in Sebes-Körös River (Crişul Repede) in eastern Hungary and western Romania and is formally described here as B. biharicus Antal, László, Kotlík - sp. nov.
authors.affiliation: Department of Hydrobiology, Faculty of Science and Technology, University of Debrecen, Debrecen, Hungary. Electronic address: antal.laszlo@science.unideb.hu.
authors.forename: László
authors.initials: L
authors.lastname: Antal
authors.affiliation: Department of Medical Microbiology, University of Debrecen, Debrecen, Hungary.
authors.forename: Brigitta
authors.initials: B
authors.lastname: László
authors.affiliation: Laboratory of Molecular Ecology, Institute of Animal Physiology and Genetics, The Czech Academy of Sciences, Liběchov, Czech Republic.
authors.forename: Petr
authors.initials: P
authors.lastname: Kotlík
authors.affiliation: Department of Hydrobiology, Faculty of Science and Technology, University of Debrecen, Debrecen, Hungary; Balaton Limnological Institute, MTA Centre for Ecological Research, Tihany, Hungary.
authors.forename: Attila
authors.initials: A
authors.lastname: Mozsár
authors.affiliation: Department of Hydrobiology, Faculty of Science and Technology, University of Debrecen, Debrecen, Hungary; Balaton Limnological Institute, MTA Centre for Ecological Research, Tihany, Hungary.
authors.forename: István
authors.initials: I
authors.lastname: Czeglédi
authors.affiliation: Virological Research Group, Szentágothai Research Center, University of Pécs, Pécs, Hungary.
authors.forename: Miklós
authors.initials: M
authors.lastname: Oldal
authors.affiliation: Virological Research Group, Szentágothai Research Center, University of Pécs, Pécs, Hungary.
authors.forename: Gábor
authors.initials: G
authors.lastname: Kemenesi
authors.affiliation: Virological Research Group, Szentágothai Research Center, University of Pécs, Pécs, Hungary.
authors.forename: Ferenc
authors.initials: F
authors.lastname: Jakab
authors.affiliation: Department of Hydrobiology, Faculty of Science and Technology, University of Debrecen, Debrecen, Hungary.
authors.forename: Sándor Alex
authors.initials: SA
authors.lastname: Nagy
mesh_terms: D000818:Animals; D063368:Balkan Peninsula; D002467:Cell Nucleus / Q000235:genetics; D003530:Cyprinidae / Q000033:anatomy & histology / Q000145:classification* / Q000235:genetics*; D005260:Female; D050259:Genes, Mitochondrial / Q000235:genetics; D006814:Hungary; D008297:Male; D010802:Phylogeny; D045483:Rivers; D012383:Romania; D013045:Species Specificity
keywords: ATPase 6/8; Act-2; Barbus biharicus; Biharian barbel; Cyprinidae; Cyt b