title: Fabrication of silk mesh with enhanced cytocompatibility: preliminary in vitro investigation toward cell-based therapy for hernia repair.
abstract: Recent studies have demonstrated that combining cells with meshes prior to implantation successfully enhanced hernia repair. The idea is to create a biologic coating surrounding the mesh with autologous cells, before transplantation into the patient. However, due to the lack of a prompt and robust cell adhesion to the meshes, extensive in vitro cultivation is required to obtain a homogenous cell layer covering the mesh. In this context, the objective of this publication is to manufacture meshes made of silk fibres and to enhance the cytoadhesion and cytocompatibility of the biomaterial by surface immobilization of a pro-adhesive wheat germ agglutinin (lectin WGA). We first investigated the affinity between the glycoprotein WGA and cells, in solution and then after covalent immobilization of WGA on silk films. Then, we manufactured meshes made of silk fibres, tailored them with WGA grafting and finally evaluated the cytocompatibility and the inflammatory response of silk and silk-lectin meshes compared to common polypropylene mesh, using fibroblasts and peripheral blood mononuclear cells, respectively. The in vitro experiments revealed that the cytocompatibility of silk can be enhanced by surface immobilization with lectin WGA without exhibiting negative response in terms of pro-inflammatory reaction. Grafting lectin to silk meshes could bring advantages to facilitate cell-coating of meshes prior to implantation, which is an imperative prerequisite for abdominal wall tissue regeneration using cell-based therapy.
authors.affiliation: Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, Donaueschingenstrasse 13, 1200, Vienna, Austria. olivier.guillaume@trauma.lbg.ac.at.|The Austrian Cluster for Tissue Regeneration, Vienna, Austria. olivier.guillaume@trauma.lbg.ac.at.
authors.forename: O
authors.initials: O
authors.lastname: Guillaume
authors.affiliation: Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, Donaueschingenstrasse 13, 1200, Vienna, Austria.|The Austrian Cluster for Tissue Regeneration, Vienna, Austria.
authors.forename: J
authors.initials: J
authors.lastname: Park
authors.affiliation: Department of Biochemical Engineering, University of Applied Sciences Technikum Wien, Höchstädtplatz 5, 1200, Vienna, Austria.|The Austrian Cluster for Tissue Regeneration, Vienna, Austria.
authors.forename: X
authors.initials: X
authors.lastname: Monforte
authors.affiliation: Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, Donaueschingenstrasse 13, 1200, Vienna, Austria.|Department of General, Visceral and Oncological Surgery, Wilhelminenspital der Stadt Wien, Montleartstrasse 37, 1171, Vienna, Austria.
authors.forename: S
authors.initials: S
authors.lastname: Gruber-Blum
authors.affiliation: Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, Donaueschingenstrasse 13, 1200, Vienna, Austria.|The Austrian Cluster for Tissue Regeneration, Vienna, Austria.
authors.forename: H
authors.initials: H
authors.lastname: Redl
authors.affiliation: Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, Donaueschingenstrasse 13, 1200, Vienna, Austria.|Department of General, Visceral and Oncological Surgery, Wilhelminenspital der Stadt Wien, Montleartstrasse 37, 1171, Vienna, Austria.|The Austrian Cluster for Tissue Regeneration, Vienna, Austria.
authors.forename: A
authors.initials: A
authors.lastname: Petter-Puchner
authors.affiliation: Department of Biochemical Engineering, University of Applied Sciences Technikum Wien, Höchstädtplatz 5, 1200, Vienna, Austria.|The Austrian Cluster for Tissue Regeneration, Vienna, Austria.
authors.forename: A H
authors.initials: AH
authors.lastname: Teuschl
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis* / Q000737:chemistry; D012831:Bombyx; D064987:Cell- and Tissue-Based Therapy / Q000295:instrumentation / Q000379:methods; D059685:Herniorrhaphy / Q000295:instrumentation / Q000379:methods; D006801:Humans; D008422:Materials Testing; D051379:Mice; D055616:Microtechnology / Q000379:methods*; D041681:NIH 3T3 Cells; D010865:Pilot Projects; D047011:Silk / Q000138:chemical synthesis / Q000737:chemistry*; D013526:Surgical Mesh
chemical_list: D001672:Biocompatible Materials; D047011:Silk