title: Structural and Functional Insight into Proliferating Cell Nuclear Antigen.
abstract: Proliferating cell nuclear antigen (PCNA) is a critical eukaryotic replication accessory factor that supports DNA binding in DNA processing, such as DNA replication, repair, and recombination. PCNA consists of three toroidal-shaped monomers that encircle doublestranded DNA. The diverse functions of PCNA may be regulated by its interactions with partner proteins. Many of the PCNA partner proteins generally have a conserved PCNAinteracting peptide (PIP) motif, located at the N- or C- terminal region. The PIP motif forms a 310 helix that enters into the hydrophobic groove produced by an interdomain-connecting loop, a central loop, and a C-terminal tail in the PCNA. Post-translational modification of PCNA also plays a critical role in regulation of its function and binding partner proteins. Structural and biochemical studies of PCNA-protein will be useful in designing therapeutic agents, as well as estimating the outcome of anticancer drug development. This review summarizes the characterization of eukaryotic PCNA in relation to the protein structures, functions, and modifications, and interaction with proteins.
authors.affiliation: Department of Molecular Biology, College of Natural Sciences, Pusan National University, Busan 46241, Republic of Korea.|Department of Parasitology, School of Medicine, Pusan National University, Yangsan 50612, Republic of Korea.
authors.forename: So Young
authors.initials: SY
authors.lastname: Park
authors.affiliation: Department of Molecular Biology, College of Natural Sciences, Pusan National University, Busan 46241, Republic of Korea.
authors.forename: Mi Suk
authors.initials: MS
authors.lastname: Jeong
authors.affiliation: Department of Molecular Biology, College of Natural Sciences, Pusan National University, Busan 46241, Republic of Korea.
authors.forename: Chang Woo
authors.initials: CW
authors.lastname: Han
authors.affiliation: Department of Parasitology, School of Medicine, Pusan National University, Yangsan 50612, Republic of Korea.
authors.forename: Hak Sun
authors.initials: HS
authors.lastname: Yu
authors.affiliation: Department of Molecular Biology, College of Natural Sciences, Pusan National University, Busan 46241, Republic of Korea.
authors.forename: Se Bok
authors.initials: SB
authors.lastname: Jang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D004261:DNA Replication; D004268:DNA-Binding Proteins / Q000737:chemistry; D006801:Humans; D018809:Proliferating Cell Nuclear Antigen / Q000737:chemistry* / Q000235:genetics / Q000502:physiology*; D011485:Protein Binding; D011499:Protein Processing, Post-Translational / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics
chemical_list: D004268:DNA-Binding Proteins; D018809:Proliferating Cell Nuclear Antigen; D029701:Saccharomyces cerevisiae Proteins
keywords: DNA; PCNA; function; interaction; structure