title: Requirement for nuclear autoantigenic sperm protein mRNA expression in bovine preimplantation development.
abstract: Nuclear autoantigenic sperm protein (NASP) is associated with DNA replication, cell proliferation, and cell cycle progression through its specific binding to histones. The aim of this study was to examine the roles of NASP in bovine preimplantation embryonic development. Using NASP gene knockdown (KD), we confirmed the reduction of NASP messenger RNA (mRNA) expression during preimplantation development. NASP KD did not affect cleavage but significantly decreased development of embryos into the blastocyst stage. Furthermore, blastocyst hatching was significantly decreased in NASP KD embryos. Cell numbers in the inner cell mass of NASP KD blastocysts were also decreased compared to those of controls. These results suggest that NASP mRNA expression is required for preimplantation development into the blastocyst stage in cattle.
authors.affiliation: Advanced Biotechnology Center, University of Yamanashi, Kofu, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Nagatomo
authors.affiliation: Laboratory of Animal Breeding and Reproduction, Graduate School of Agriculture, Hokkaido University, Sapporo, Japan.
authors.forename: Nanami
authors.initials: N
authors.lastname: Kohri
authors.affiliation: Laboratory of Animal Breeding and Reproduction, Graduate School of Agriculture, Hokkaido University, Sapporo, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Akizawa
authors.affiliation: Laboratory of Animal Reproduction, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
authors.forename: Yumi
authors.initials: Y
authors.lastname: Hoshino
authors.affiliation: Department of Animal and Marine Bioresource Sciences, Graduate School of Agriculture, Kyushu University, Fukuoka, Japan.
authors.forename: Nobuhiko
authors.initials: N
authors.lastname: Yamauchi
authors.affiliation: Department of BioScience, Tokyo University of Agriculture, Tokyo, Japan.
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Kono
authors.affiliation: Laboratory of Animal Breeding and Reproduction, Graduate School of Agriculture, Hokkaido University, Sapporo, Japan.
authors.forename: Masashi
authors.initials: M
authors.lastname: Takahashi
authors.affiliation: Laboratory of Animal Breeding and Reproduction, Graduate School of Agriculture, Hokkaido University, Sapporo, Japan.
authors.forename: Manabu
authors.initials: M
authors.lastname: Kawahara
mesh_terms: D000818:Animals; D001324:Autoantigens / Q000235:genetics*; D002417:Cattle; D047108:Embryonic Development / Q000235:genetics*; D005260:Female; D015870:Gene Expression / Q000235:genetics*; D008297:Male; D009687:Nuclear Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*
chemical_list: D001324:Autoantigens; D009687:Nuclear Proteins; D012333:RNA, Messenger
keywords: NASP; bovine embryo; knockdown