title: Resveratrol Prevents Retinal Dysfunction by Regulating Glutamate Transporters, Glutamine Synthetase Expression and Activity in Diabetic Retina.
abstract: This study investigated the effects of resveratrol (RSV) on retinal functions, glutamate transporters (GLAST) and glutamine synthetase (GS) expression in diabetic rats retina, and on glutamate uptake, GS activity, GLAST and GS expression in high glucose-cultured Müller cells. The electroretinogram was used to evaluate retinal functions. Müller cells cultures were prepared from 5- to 7-day-old Sprague-Dawley rats. The expression of GLAST and GS was examined by qRT-PCR, ELISA and western-blotting. Glutamate uptake was measured as (3)H-glutamate contents of the lysates. GS activity was assessed by a spectrophotometric assay. 1- to 7-month RSV administrations (5 and 10 mg/kg/day) significantly alleviated hyperglycemia and weight loss in diabetic rats. RSV administrations also significantly attenuated diabetes-induced decreases in amplitude of a-wave in rod response, decreases in amplitude of a-, and b-wave in cone and rod response and decreases in amplitude of OP2 in oscillatory potentials. 1- to 7-month RSV treatments also significantly inhibited diabetes-induced delay in OP2 implicit times in scotopic 3.0 OPS test. The down-regulated mRNA and protein expression of GLAST and GS in diabetic rats retina was prevented by RSV administrations. In high glucose-treated cultures, Müller cells' glutamate uptake, GS activity, GLAST and GS expression were decreased significantly compared with normal control cultures. RSV (10, 20, and 30 mmol/l) significantly inhibited the HG-induced decreases in glutamate uptake, GS activity, GLAST and GS expression (at least P < 0.05). These beneficial results suggest that RSV may be considered as a therapeutic option to prevent from diabetic retinopathy.
authors.affiliation: Department of Clinical Nutrition, Sichuan Academy of Medical Sciences and Sichuan Provincial People's Hospital, Chengdu, 610072, People's Republic of China. zengkaihong2013@hotmail.com.
authors.forename: Kaihong
authors.initials: K
authors.lastname: Zeng
authors.affiliation: Institute of Laboratory Animals, Sichuan Academy of Medical Sciences and Sichuan Provincial People's Hospital, Chengdu, 610072, People's Republic of China.
authors.forename: Na
authors.initials: N
authors.lastname: Yang
authors.affiliation: Department of Neurology, Sichuan Academy of Medical Sciences and Sichuan Provincial People's Hospital, Chengdu, 610072, People's Republic of China.
authors.forename: Duozi
authors.initials: D
authors.lastname: Wang
authors.affiliation: Department of Neurology, Sichuan Academy of Medical Sciences and Sichuan Provincial People's Hospital, Chengdu, 610072, People's Republic of China.
authors.forename: Suping
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Food Science, Southwest University, Chongqing, 400715, People's Republic of China.
authors.forename: Jian
authors.initials: J
authors.lastname: Ming
authors.affiliation: Department of Clinical Nutrition, Sichuan Academy of Medical Sciences and Sichuan Provincial People's Hospital, Chengdu, 610072, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Clinical Nutrition, Sichuan Academy of Medical Sciences and Sichuan Provincial People's Hospital, Chengdu, 610072, People's Republic of China.
authors.forename: Xuemei
authors.initials: X
authors.lastname: Yu
authors.affiliation: Department of Clinical Nutrition, Sichuan Academy of Medical Sciences and Sichuan Provincial People's Hospital, Chengdu, 610072, People's Republic of China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Song
authors.affiliation: Department of Clinical Nutrition, Sichuan Academy of Medical Sciences and Sichuan Provincial People's Hospital, Chengdu, 610072, People's Republic of China.
authors.forename: Xue
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Department of Clinical Nutrition, Sichuan Academy of Medical Sciences and Sichuan Provincial People's Hospital, Chengdu, 610072, People's Republic of China.
authors.forename: Yongtao
authors.initials: Y
authors.lastname: Yang
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003930:Diabetic Retinopathy / Q000188:drug therapy* / Q000378:metabolism / Q000503:physiopathology; D004596:Electroretinography; D063928:Ependymoglial Cells / Q000187:drug effects / Q000378:metabolism; D027341:Excitatory Amino Acid Transporter 1 / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D005974:Glutamate-Ammonia Ligase / Q000378:metabolism*; D017207:Rats, Sprague-Dawley; D000077185:Resveratrol; D012160:Retina / Q000187:drug effects* / Q000378:metabolism / Q000503:physiopathology; D013267:Stilbenes / Q000494:pharmacology*
chemical_list: D027341:Excitatory Amino Acid Transporter 1; C493392:Slc1a3 protein, rat; D013267:Stilbenes; D005974:Glutamate-Ammonia Ligase; D005947:Glucose; D000077185:Resveratrol
keywords: Activity; Diabetes; Diabetic retinopathy; Expression; Glutamate transporters; Glutamine synthetase; Müller cells; Resveratrol; Retinal dysfunction