title: Transcriptomic analysis of the white rot fungus Polyporus brumalis provides insight into sesquiterpene biosynthesis.
abstract: Object of this study was to identify genes and enzymes that are involved in sesquiterpene biosynthesis in the wood rotting fungus, Polyporus brumalis. Sesquiterpenes, β-eudesmane and β-eudesmol, were produced by the mycelium of P. brumalis cultured in modified medium. However, theses final products were not observed when the fungus was grown in potato dextrose medium. We used next generation sequencing (NGS) to identify differentially expressed genes (DEGs) related to terpene metabolism. This approach generated 25,000 unigenes and 127 metabolic pathways that were assigned to Kyoto Encyclopedia Genes Groups (KEGG). Further analysis of samples from modified medium indicated significant upregulation of 8 unigenes involved in the mevalonate (MVA) and methylerythritol phosphate (MEP) biosynthetic pathways. These pathways generate isopentenyl pyrophosphate (IPP) and farnesyl pyrophosphate (FPP), which are precursors for the synthesis of sesquiterpenes. Furthermore, genes encoding germacrene A synthase, which facilitate the cyclization of FPP, were only differentially expressed in mycelium from fungi grown in modified medium. Our data provide a resource for studying the molecular mechanisms underpinning sesquiterpene biosynthesis and terpene metabolism.
authors.affiliation: Division of Wood Chemistry & Microbiology, Department of Forest Resources Utilization, Korea Forest Research Institute, Seoul 130-712, Republic of Korea.
authors.forename: Su-Yeon
authors.initials: SY
authors.lastname: Lee
authors.affiliation: Division of Wood Chemistry & Microbiology, Department of Forest Resources Utilization, Korea Forest Research Institute, Seoul 130-712, Republic of Korea.
authors.forename: Myungkil
authors.initials: M
authors.lastname: Kim
authors.affiliation: Department of Forest Sciences, College of Agriculture and Life Sciences, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 151-921, South Korea.
authors.forename: Seon-Hong
authors.initials: SH
authors.lastname: Kim
authors.affiliation: Department of Forest Sciences, College of Agriculture and Life Sciences, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 151-921, South Korea.
authors.forename: Chang-Young
authors.initials: CY
authors.lastname: Hong
authors.affiliation: Division of Wood Chemistry & Microbiology, Department of Forest Resources Utilization, Korea Forest Research Institute, Seoul 130-712, Republic of Korea. Electronic address: shryu@forest.go.kr.
authors.forename: Sun-Hwa
authors.initials: SH
authors.lastname: Ryu
authors.affiliation: Department of Forest Sciences, College of Agriculture and Life Sciences, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 151-921, South Korea. Electronic address: cingyu@snu.ac.kr.
authors.forename: In-Gyu
authors.initials: IG
authors.lastname: Choi
mesh_terms: D053898:Biosynthetic Pathways; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D010935:Plant Diseases / Q000382:microbiology*; D055492:Polyporus / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D012717:Sesquiterpenes / Q000378:metabolism*; D059467:Transcriptome
chemical_list: D005656:Fungal Proteins; D012717:Sesquiterpenes
keywords: Methylerythritol phosphate pathway; Mevalonate pathway; Polyporus brumalis; Sesquiterpene; Transcriptome