title: Improved ex vivo blood compatibility of central venous catheter with noble metal alloy coating.
abstract: Central line associated bloodstream infections (CLABSIs) are a serious cause of morbidity and mortality induced by the use of central venous catheters (CVCs). Nobel metal alloy (NMA) coating is an advanced surface modification that prevents microbial adhesion and growth on catheters and thereby reduces the risk of infection. In vitro microbiological analyses have shown up to 90% reduction in microbial adhesion on coated CVC compared to uncoated ones. This study aimed to assess the blood compatibility of NMA-coated CVC according to ISO 10993-4. Hemolysis, thrombin-antithrombin (TAT) complex, platelet counts, fibrin deposition, and C3a and SC5b-9 complement activation were analyzed in human blood exposed to the NMA-coated and control CVCs using a Chandler-loop model. NMA-coated CVC did not induce hemolysis and fell in the "nonhemolytic" category according to ASTM F756-00. Significantly lower amounts of TAT were generated and less fibrin was deposited on NMA-coated CVC than on uncoated ones. Slightly higher platelet counts and lower complement markers were observed for NMA-coated CVC compared to uncoated ones. These data suggest that the NMA-coated CVC has better ex vivo blood compatibility compared to uncoated CVC. © 2015 The Authors Journal of Biomedical Materials Research Part B: Applied Biomaterials Published by Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 104B: 1359-1365, 2016.
authors.affiliation: Infectious Disease Unit, Karolinska University Hospital, Stockholm, Sweden.
authors.forename: Manijeh
authors.initials: M
authors.lastname: Vafa Homann
authors.affiliation: Research and Development Department, Bactiguard AB, Stockholm, Sweden.
authors.forename: Dorota
authors.initials: D
authors.lastname: Johansson
authors.affiliation: Division of internal and Cardiovascular Medicine, Department of Clinical Science Danderyd Hospital, Karolinska Institutet, Danderyd Hospital, Stockholm, Sweden.
authors.forename: Håkan
authors.initials: H
authors.lastname: Wallen
authors.affiliation: Research and Development Department, Bactiguard AB, Stockholm, Sweden. javier.sanchez@bactiguard.se.|Division of internal and Cardiovascular Medicine, Department of Clinical Science Danderyd Hospital, Karolinska Institutet, Danderyd Hospital, Stockholm, Sweden. javier.sanchez@bactiguard.se.
authors.forename: Javier
authors.initials: J
authors.lastname: Sanchez
mesh_terms: D000328:Adult; D000497:Alloys / Q000737:chemistry*; D001792:Blood Platelets / Q000378:metabolism*; D001798:Blood Proteins / Q000737:chemistry* / Q000378:metabolism; D062905:Central Venous Catheters; D005260:Female; D006801:Humans; D008297:Male; D008422:Materials Testing
chemical_list: D000497:Alloys; D001798:Blood Proteins
keywords: blood compatibility; central Venous catheter; central line associated blood stream infection; nobel metal alloy coating; surface modification