title: The Effects of Bariatric Surgery-Induced Weight Loss on Adipose Tissue in Morbidly Obese Women Depends on the Initial Metabolic Status.
abstract: Adipose tissue (AT) dysfunction in obesity is commonly linked to insulin resistance and promotes the development of metabolic disease. Bariatric surgery (BS) represents an effective strategy to reduce weight and to improve metabolic health in morbidly obese subjects. However, the mechanisms and pathways that are modified in AT in response to BS are not fully understood, and few information is still available as to whether these may vary depending on the metabolic status of obese subjects.
  
  
  
  Abdominal subcutaneous adipose tissue (SAT) samples were obtained from morbidly obese women (n = 18) before and 13.3 ± 0.37 months after BS. Obese women were stratified into two groups: normoglycemic (NG; Glu < 100 mg/dl, HbA1c <5.7 %) or insulin resistant (IR; Glu 100-126 mg/dl, HbA1c 5.7-6.4 %) (n = 9/group). A multi-comparative proteomic analysis was employed to identify differentially regulated SAT proteins by BS and/or the degree of insulin sensitivity. Serum levels of metabolic, inflammatory, and anti-oxidant markers were also analyzed.
  
  
  
  Before surgery, NG and IR subjects exhibited differences in AT proteins related to inflammation, metabolic processes, the cytoskeleton, and mitochondria. BS caused comparable weight reductions and improved glucose homeostasis in both groups. However, BS caused dissimilar changes in metabolic enzymes, inflammatory markers, cytoskeletal components, mitochondrial proteins, and angiogenesis regulators in NG and IR women.
  
  
  
  BS evokes significant molecular rearrangements indicative of improved AT function in morbidly obese women at either low or high metabolic risk, though selective adaptive changes in key cellular processes occur depending on the initial individual's metabolic status.
authors.affiliation: Department of Cell Biology, Physiology and Immunology, Instituto Maimónides de Investigación Biomédica de Córdoba (IMIBIC)/Reina Sofia University Hospital/University of Córdoba, Avda. Menéndez Pidal s/n, 14004, Córdoba, Spain.|CIBER Fisiopatología de la Obesidad y Nutrición (CIBEROBN), Instituto de Salud Carlos III, Madrid, Spain.
authors.forename: Natalia
authors.initials: N
authors.lastname: Moreno-Castellanos
authors.affiliation: Department of Cell Biology, Physiology and Immunology, Instituto Maimónides de Investigación Biomédica de Córdoba (IMIBIC)/Reina Sofia University Hospital/University of Córdoba, Avda. Menéndez Pidal s/n, 14004, Córdoba, Spain.|CIBER Fisiopatología de la Obesidad y Nutrición (CIBEROBN), Instituto de Salud Carlos III, Madrid, Spain.
authors.forename: Rocío
authors.initials: R
authors.lastname: Guzmán-Ruiz
authors.affiliation: Unidad de Gestión Clínica de Endocrinología y Nutrición, Instituto de Biomedicina de Sevilla (IBiS), Hospital Universitario Virgen del Rocío/Consejo Superior de Investigaciones Científícas/Universidad de Sevilla, Avda. Manuel Siurot, s/n, Sevilla, 41013, Spain.
authors.forename: David A
authors.initials: DA
authors.lastname: Cano
authors.affiliation: Unidad de Gestión Clínica de Endocrinología y Nutrición, Instituto de Biomedicina de Sevilla (IBiS), Hospital Universitario Virgen del Rocío/Consejo Superior de Investigaciones Científícas/Universidad de Sevilla, Avda. Manuel Siurot, s/n, Sevilla, 41013, Spain.
authors.forename: Ainara
authors.initials: A
authors.lastname: Madrazo-Atutxa
authors.affiliation: Department of Cell Biology, Physiology and Immunology, Instituto Maimónides de Investigación Biomédica de Córdoba (IMIBIC)/Reina Sofia University Hospital/University of Córdoba, Avda. Menéndez Pidal s/n, 14004, Córdoba, Spain.|CIBER Fisiopatología de la Obesidad y Nutrición (CIBEROBN), Instituto de Salud Carlos III, Madrid, Spain.
authors.forename: Juan R
authors.initials: JR
authors.lastname: Peinado
authors.affiliation: Unidad de Gestión Clínica de Endocrinología y Nutrición, Instituto de Biomedicina de Sevilla (IBiS), Hospital Universitario Virgen del Rocío/Consejo Superior de Investigaciones Científícas/Universidad de Sevilla, Avda. Manuel Siurot, s/n, Sevilla, 41013, Spain.
authors.forename: Jose L
authors.initials: JL
authors.lastname: Pereira-Cunill
authors.affiliation: Unidad de Gestión Clínica de Endocrinología y Nutrición, Instituto de Biomedicina de Sevilla (IBiS), Hospital Universitario Virgen del Rocío/Consejo Superior de Investigaciones Científícas/Universidad de Sevilla, Avda. Manuel Siurot, s/n, Sevilla, 41013, Spain.
authors.forename: Pedro Pablo
authors.initials: PP
authors.lastname: García-Luna
authors.affiliation: Unit of Innovation in Minimally Invasive Surgery, Unidad de Gestión Clínica de Cirugía, Hospital Universitario Virgen del Rocío, Sevilla, Spain.
authors.forename: Salvador
authors.initials: S
authors.lastname: Morales-Conde
authors.affiliation: Unit of Innovation in Minimally Invasive Surgery, Unidad de Gestión Clínica de Cirugía, Hospital Universitario Virgen del Rocío, Sevilla, Spain.
authors.forename: Maria
authors.initials: M
authors.lastname: Socas-Macias
authors.affiliation: Department of Cell Biology, Physiology and Immunology, Instituto Maimónides de Investigación Biomédica de Córdoba (IMIBIC)/Reina Sofia University Hospital/University of Córdoba, Avda. Menéndez Pidal s/n, 14004, Córdoba, Spain.|CIBER Fisiopatología de la Obesidad y Nutrición (CIBEROBN), Instituto de Salud Carlos III, Madrid, Spain.
authors.forename: Rafael
authors.initials: R
authors.lastname: Vázquez-Martínez
authors.affiliation: Unidad de Gestión Clínica de Endocrinología y Nutrición, Instituto de Biomedicina de Sevilla (IBiS), Hospital Universitario Virgen del Rocío/Consejo Superior de Investigaciones Científícas/Universidad de Sevilla, Avda. Manuel Siurot, s/n, Sevilla, 41013, Spain. alealcerro@us.es.
authors.forename: Alfonso
authors.initials: A
authors.lastname: Leal-Cerro
authors.affiliation: Department of Cell Biology, Physiology and Immunology, Instituto Maimónides de Investigación Biomédica de Córdoba (IMIBIC)/Reina Sofia University Hospital/University of Córdoba, Avda. Menéndez Pidal s/n, 14004, Córdoba, Spain. bc1mapom@uco.es.|CIBER Fisiopatología de la Obesidad y Nutrición (CIBEROBN), Instituto de Salud Carlos III, Madrid, Spain. bc1mapom@uco.es.
authors.forename: María M
authors.initials: MM
authors.lastname: Malagón
mesh_terms: D000328:Adult; D050110:Bariatric Surgery; D015415:Biomarkers / Q000378:metabolism*; D005260:Female; D006801:Humans; D007333:Insulin Resistance; D024821:Metabolic Syndrome / Q000378:metabolism*; D009767:Obesity, Morbid / Q000378:metabolism / Q000601:surgery*; D050507:Subcutaneous Fat, Abdominal / Q000378:metabolism*; D015431:Weight Loss; D016387:Women's Health
chemical_list: D015415:Biomarkers
keywords: Adipose tissue; Bariatric surgery; Insulin resistance; Proteomics; Weight loss