title: Downregulation of SATB1 increases the invasiveness of Jurkat cell via activation of the WNT/β-catenin signaling pathway in vitro.
abstract: Special AT-rich sequence-binding protein-1 (SATB1) is critical for genome organizer that reprograms chromatin organization and transcription profiles, and associated with tumor growth and metastasis in several cancer types. Many studies suggest that SATB1 overexpression is an indicator of poor prognosis in various cancers, such as breast cancer, malignant cutaneous melanoma, and liver cancer. However, their expression patterns and function values for adult T cell leukemia (ATL) are still largely unknown. The aim of this study is to examine the levels of SATB1 in ATL and to explore its function and mechanisms in Jurkat cell line. Here, we reported that SATB1 expressions were decreased in ATL cells (p < 0.001) compared with normal controls. Knockdown of SATB1 expression significantly enhanced invasion of Jurkat cell in vitro. Furthermore, knockdown of SATB1 gene enhances β-catenin nuclear accumulation and transcriptional activity and thus may increase the invasiveness of Jurkat cell through the activation of Wnt/β-catenin signaling pathway in vitro.
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510230, People's Republic of China.
authors.forename: Xiao-Dan
authors.initials: XD
authors.lastname: Luo
authors.affiliation: Department of Hematology, The First People's Hospital of Foshan, Foshan, 528000, China.
authors.forename: Shao-Jiang
authors.initials: SJ
authors.lastname: Yang
authors.affiliation: Breast Cancer Center, The third Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.
authors.forename: Jia-Ni
authors.initials: JN
authors.lastname: Wang
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510230, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Tan
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510230, People's Republic of China.
authors.forename: Dan
authors.initials: D
authors.lastname: Liu
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510230, People's Republic of China.
authors.forename: Ya-Ya
authors.initials: YY
authors.lastname: Wang
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510230, People's Republic of China.
authors.forename: Run-Hui
authors.initials: RH
authors.lastname: Zheng
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510230, People's Republic of China.
authors.forename: Xiao-Hong
authors.initials: XH
authors.lastname: Wu
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510230, People's Republic of China. xlhua325@126.com.
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Xu
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510230, People's Republic of China. tanhuo2008@126.com.
authors.forename: Huo
authors.initials: H
authors.lastname: Tan
mesh_terms: D000328:Adult; D045744:Cell Line, Tumor; D015536:Down-Regulation; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D019169:Jurkat Cells; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000097:blood / Q000235:genetics / Q000473:pathology; D036961:Matrix Attachment Region Binding Proteins / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D009361:Neoplasm Invasiveness; D009363:Neoplasm Proteins / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D034622:RNA Interference; D012333:RNA, Messenger / Q000096:biosynthesis; D012334:RNA, Neoplasm / Q000096:biosynthesis; D034741:RNA, Small Interfering / Q000235:genetics; D060449:Wnt Signaling Pathway / Q000235:genetics / Q000502:physiology*
chemical_list: D036961:Matrix Attachment Region Binding Proteins; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D034741:RNA, Small Interfering; C076189:SATB1 protein, human
keywords: ATL; Invasion; SATB1; β-Catenin