title: Purification and characterization of an extracellular cellulase from Anoxybacillus gonensis O9 isolated from geothermal area in Turkey.
abstract: In the present study, cellulase was purified and characterized from Anoxybacillus gonensis (Gen bank Number: KM596794) which was isolated and characterized from Agri Diyadin Hot spring. It was found to synthesize cellulase which had a wide range of industrial applications. Twenty four-hour-cultured bacteria induced cellulase production and specific activities during the purification steps were 1.47, 81.06 and 109.4 EU mg(-1) protein at crude extract, ammonium sulphate precipitated and DEAE-Sephadex purification steps. The highest enzyme activity was observed at 50°C and the optimum range of pH was 3-10. Molecular weight of enzyme was determined approximately 40kDa. The kinetic parameters of cellulase against carboxymethylcellulose (CMC) were 153.4 pmol min(-1) mg for Vmax and 0.46mM for Km. Among effectors of the enzyme, Zn2+, Ca2+, Co2+ and EDTA decreased enzyme activity.
authors.forename: Berna
authors.initials: B
authors.lastname: Genc
authors.forename: Hayrunnisa
authors.initials: H
authors.lastname: Nadaroglu
authors.forename: Ahmet
authors.initials: A
authors.lastname: Adiguzel
authors.forename: Ozkan
authors.initials: O
authors.lastname: Baltaci
mesh_terms: D058677:Anoxybacillus / Q000201:enzymology* / Q000378:metabolism; D044602:Cellulases / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology; D045482:Hot Springs; D007700:Kinetics; D013379:Substrate Specificity; D013696:Temperature
chemical_list: D044602:Cellulases