title: The pathogenic potential of Helicobacter cinaedi isolated from non-human sources: adherence, invasion and translocation ability in polarized intestinal epithelial Caco-2 cells in vitro.
abstract: Helicobacter cinaedi infection has been recognized as an increasingly important emerging disease in humans. Infection with H. cinaedi causes bacteremia, cellulitis and enteritis. H. cinaedi has been isolated from non-human sources, including dogs, cats and rodents; however, it remains unclear whether animal strains are pathogenic in humans and as zoonotic pathogens. In this study, H. cinaedi isolates were recovered from a dog and a hamster, and the ability of these isolates to adhere to, invade and translocate across polarized human intestinal epithelial Caco-2 cells was examined in vitro. To better understand the pathogenic potential of animal H. cinaedi isolates, these results were compared with those for a human strain that was isolated from a patient with bacteremia. The animal and human strains adhered to and invaded Caco-2 cells, but to a lesser degree than the C. jejuni 81-176 strain, which was used as a control. The integrity of tight junctions was monitored by measuring transepithelial electrical resistance (TER) with a membrane insert system. The TER values for all H. cinaedi strains did not change during the experimental periods compared with those of the controls; however, translocation of H. cinaedi from the apical side to the basolateral side was confirmed by cultivation and H. cinaedi-specific PCR, suggesting that the H. cinaedi strains translocated by transcellular route. This study demonstrated that H. cinaedi strains of animal origin might have a pathogenic potential in human epithelial cells as observed in a translocation assay in vitro with a human isolate.
authors.affiliation: Laboratory of Veterinary Public Health, Department of Veterinary Medical Science, Faculty of Agriculture, University of Miyazaki, 1-1 Gakuenkibanadai-nishi, Miyazaki 889-2192, Japan.
authors.forename: Takako
authors.initials: T
authors.lastname: Taniguchi
authors.forename: Wataru
authors.initials: W
authors.lastname: Yamazaki
authors.forename: Yuji
authors.initials: Y
authors.lastname: Saeki
authors.forename: Ichiro
authors.initials: I
authors.lastname: Takajo
authors.forename: Akihiko
authors.initials: A
authors.lastname: Okayama
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Hayashi
authors.forename: Naoaki
authors.initials: N
authors.lastname: Misawa
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion; D018938:Caco-2 Cells; D016764:Cell Polarity; D006224:Cricetinae; D004285:Dogs; D016998:Helicobacter / Q000302:isolation & purification / Q000472:pathogenicity*; D006801:Humans; D007413:Intestinal Mucosa / Q000382:microbiology*