title: Development of a hydrophilic interaction liquid chromatography-tandem mass spectrometric method for the determination of kinsenoside, an antihyperlipidemic candidate, in rat plasma and its application to pharmacokinetic studies.
abstract: Kinsenoside is a major bioactive constituent isolated from Anoectochilus formosanus and is investigated as an antihyperlipidemic candidate. In this study, a rapid, sensitive, and reliable bioanalytical method was developed for the determination of kinsenoside in rat plasma using hydrophilic interaction liquid chromatography-tandem mass spectrometry (HILIC-MS/MS). The plasma sample was pretreated with 1% acetic acid, followed by protein precipitation with acetonitrile:methanol (70:30). Chromatographic separation was performed on a HILIC silica column (2.1mm×100mm, 3μm). The mobile phases consisted of 0.1% acetic acid in distilled water (solvent A) and 0.1% acetic acid in acetonitrile (solvent B). A gradient program was used at a flow rate of 0.2mL/min. For mass spectrometric detection, the multiple reaction monitoring mode was used; the MRM transitions were m/z 265.2→m/z 102.9 for kinsenoside and m/z 163.3→m/z 132.1 for the internal standard (IS) nicotine in the positive ionization mode. A calibration curve was constructed in the range of 2-500ng/mL. The intra- and interday precision and accuracy were within 5%. The HILIC-MS/MS method was specific, accurate, and reproducible and was successfully applied in a pharmacokinetic study of kinsenoside in rats.
authors.affiliation: Institute of Pharmaceutical Science and Technology and College of Pharmacy, Hanyang University, Ansan, Republic of Korea.
authors.forename: Shaheed Ur
authors.initials: SU
authors.lastname: Rehman
authors.affiliation: Institute of Pharmaceutical Science and Technology and College of Pharmacy, Hanyang University, Ansan, Republic of Korea.
authors.forename: In Sook
authors.initials: IS
authors.lastname: Kim
authors.affiliation: Institute of Pharmaceutical Science and Technology and College of Pharmacy, Hanyang University, Ansan, Republic of Korea.
authors.forename: Min Sun
authors.initials: MS
authors.lastname: Choi
authors.affiliation: School of Pharmacy, Tongji Medical College of Huazhong University of Science and Technology, China.
authors.forename: Zengwei
authors.initials: Z
authors.lastname: Luo
authors.affiliation: School of Pharmacy, Tongji Medical College of Huazhong University of Science and Technology, China.
authors.forename: Guangming
authors.initials: G
authors.lastname: Yao
authors.affiliation: School of Pharmacy, Tongji Medical College of Huazhong University of Science and Technology, China.
authors.forename: Yongbo
authors.initials: Y
authors.lastname: Xue
authors.affiliation: School of Pharmacy, Tongji Medical College of Huazhong University of Science and Technology, China.
authors.forename: Yonghui
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Institute of Pharmaceutical Science and Technology and College of Pharmacy, Hanyang University, Ansan, Republic of Korea. Electronic address: yoohh@hanyang.ac.kr.
authors.forename: Hye Hyun
authors.initials: HH
authors.lastname: Yoo
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics; D000818:Animals; D002138:Calibration; D002853:Chromatography, Liquid / Q000379:methods*; D057927:Hydrophobic and Hydrophilic Interactions; D000960:Hypolipidemic Agents / Q000097:blood* / Q000493:pharmacokinetics*; D009005:Monosaccharides / Q000097:blood* / Q000493:pharmacokinetics*; D010949:Plasma / Q000737:chemistry*; D051381:Rats; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: C422363:3-glucopyranosyloxybutanolide; D000960:Hypolipidemic Agents; D009005:Monosaccharides; D015107:4-Butyrolactone
keywords: Antihyperlipidemic agent; Kinsenoside; LC–MS/MS; Pharmacokinetics; Plasma