title: Identification of Novel Chromosomal Aberrations Induced by (60)Co-γ-Irradiation in Wheat-Dasypyrum villosum Lines.
abstract: Mutations induced by radiation are widely used for developing new varieties of plants. To better understand the frequency and pattern of irradiation-induced chromosomal rearrangements, we irradiated the dry seeds of Chinese Spring (CS)-Dasypyrum villosum nullisomic-tetrasomic (6A/6D) addition (6V) line (2n = 44), WD14, with (60)Co-γ-rays at dosages of 100, 200, and 300 Gy. The M₀ and M₁ generations were analyzed using Feulgen staining and non-denaturing fluorescence in situ hybridization (ND-FISH) by using oligonucleotide probes. Abnormal mitotic behavior and chromosomes with structural changes were observed in the M₀ plants. In all, 39 M₁ plants had structurally changed chromosomes, with the B genome showing the highest frequency of aberrations and tendency to recombine with chromosomes of the D genome. In addition, 19 M₁ plants showed a variation in chromosome number. The frequency of chromosome loss was considerably higher for 6D than for the alien chromosome 6V, indicating that 6D is less stable after irradiation. Our findings suggested that the newly obtained γ-induced genetic materials might be beneficial for future wheat breeding programs and functional gene analyses.
authors.affiliation: Institute of Biotechnology and Nuclear Technology Research, Sichuan Academy of Agricultural Sciences, Chengdu 610061, Sichuan, China. jade_chang@aliyun.com.|School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China. jade_chang@aliyun.com.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Institute of Biotechnology and Nuclear Technology Research, Sichuan Academy of Agricultural Sciences, Chengdu 610061, Sichuan, China. jiangyun20000@sohu.com.
authors.forename: Yun
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Institute of Biotechnology and Nuclear Technology Research, Sichuan Academy of Agricultural Sciences, Chengdu 610061, Sichuan, China. gylin63@aliyun.com.
authors.forename: Yuanlin
authors.initials: Y
authors.lastname: Guo
authors.affiliation: School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China. ligr28@uestc.edu.cn.
authors.forename: Guangrong
authors.initials: G
authors.lastname: Li
authors.affiliation: School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China. yangzujun@uestc.edu.cn.
authors.forename: Zujun
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Department of Cell Biology and Genetics, Zunyi Medical University, Zunyi 563000, Guizhou, China. xudelin@zmc.edu.cn.
authors.forename: Delin
authors.initials: D
authors.lastname: Xu
authors.affiliation: Institute of Biotechnology and Nuclear Technology Research, Sichuan Academy of Agricultural Sciences, Chengdu 610061, Sichuan, China. xuanpu64@sina.com.|Institute of Agro-Products Processing Science and Technology, Sichuan Academy of Agricultural Sciences, Chengdu 610066, Sichuan, China. xuanpu64@sina.com.
authors.forename: Pu
authors.initials: P
authors.lastname: Xuan
mesh_terms: D002869:Chromosome Aberrations / Q000528:radiation effects*; D032461:Chromosomes, Plant / Q000528:radiation effects*; D003037:Cobalt Radioisotopes / Q000737:chemistry*; D008677:Metaphase / Q000235:genetics / Q000528:radiation effects; D014178:Translocation, Genetic / Q000528:radiation effects; D014908:Triticum / Q000235:genetics* / Q000528:radiation effects*
chemical_list: D003037:Cobalt Radioisotopes
keywords: Dasypyrum villosum; ND-FISH; chromosomal aberrations; irradiation; wheat