title: Production and characterization of poly(3-hydroxy butyrate-co-3 hydroxyvalerate) (PHBV) by a novel halotolerant mangrove isolate.
abstract: A halophilic mangrove isolate identified by 16S rRNA sequence as a Bacillus spp. was found to be capable of using a broad range of carbon sources including monosaccharides (glucose and fructose), disaccharides (sucrose), pentoses (xylose and arabinose), various organic acids (acetic acid, propionic acid and octanoic acid) and even the acid pre-treated liquor (APL) of sugarcane trash, a lignocellulosic biomass, for growth and the production of polyhydroxyalkanoates (PHAs) such as poly(3-hydroxybutyrate, P3HB), poly(3-hydroxybutyrate-co-3-hydroxyvalerate, PHBV), and 4-hydroxyhexanoate, 4HHX). The study describes the innate ability of a wild-type culture for PHBV production by both propionate dependent and propionate independent pathways. The biopolymer was extracted and characterized physico-chemically. The PHBV yield from glucose was estimated to be 73% of biomass weight with a high 3-hydroxyvalerate fraction of 48mol%. Thereafter, spherical homogenous PHBV nanoparticles of ∼164nm size were prepared for future applications.
authors.affiliation: Biotechnology Division, CSIR - National Institute for Interdisciplinary Science and Technology (NIIST), Trivandrum, 695 019 Kerala, India.
authors.forename: Dhanya
authors.initials: D
authors.lastname: Moorkoth
authors.affiliation: Biotechnology Division, CSIR - National Institute for Interdisciplinary Science and Technology (NIIST), Trivandrum, 695 019 Kerala, India. Electronic address: madhavan85@hotmail.com.
authors.forename: Kesavan Madhavan
authors.initials: KM
authors.lastname: Nampoothiri
mesh_terms: D001407:Bacillus / Q000187:drug effects / Q000302:isolation & purification* / Q000378:metabolism; D018533:Biomass; D002152:Calorimetry, Differential Scanning; D002244:Carbon / Q000494:pharmacology; D006219:Halogens / Q000494:pharmacology*; D053758:Nanoparticles / Q000737:chemistry; D010421:Pentanoic Acids / Q000378:metabolism*; D010802:Phylogeny; D011091:Polyesters / Q000378:metabolism*; D011422:Propionates / Q000378:metabolism; D066244:Proton Magnetic Resonance Spectroscopy; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D031786:Saccharum / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D014866:Waste Products; D053833:Wetlands; D000094122:Polyhydroxybutyrates
chemical_list: D002244:Carbon; D006219:Halogens; D010421:Pentanoic Acids; D011091:Polyesters; D011422:Propionates; D012336:RNA, Ribosomal, 16S; D014866:Waste Products; C052620:poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate); D000094122:Polyhydroxybutyrates; C013056:beta-hydroxyvaleric acid; C029658:propionic acid
keywords: Acid pretreated liquor; Bacillus spp.; Poly(3-hydroxybutyrate) (P(3HB)); Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) P(3HB-co-3HV); Polyhydroxyalkanoates (PHAs)