title: Evaluation of gamma ray-induced gastrointestinal tract morphological and proliferative activity changes in rhesus monkeys.
abstract: To provide support for future pharmacology and preclinical studies, we have established a stable nonhuman primate animal model to demonstrate the histopathological changes in the gastrointestinal tract following gamma ray irradiation. In this study, 12 healthy rhesus monkeys were divided into 2 groups (control and radiation groups). Animals in the radiation group were exposed to gamma rays (cobalt 60 source) at a dose level of 6.5 Gy total body irradiation bilaterally (i.e. 3.25 Gy on each side). Control animals were sham exposed using identical procedures. After a 5-day in-life observation period, gastrointestinal tract tissues (esophagus, stomach, duodenum, jejunum, ileum, colon, and rectum) were collected and fixed in 10% neutral-buffered formalin for subsequent hematoxylin and eosin and 5-bromo-2-deoxyuridine (BrdU) immunohistochemistry processing. The results showed that the esophagus was undergoing degeneration without obvious inflammatory changes, while the stomach and duodenum exhibited both degeneration and inflammation. From the jejunum to the rectum, late-stage inflammation with glandular regeneration, as well as a high-level BrdU labeling index, was present.
authors.affiliation: West China School of Medicine, West China Hospital, Sichuan University, Chengdu, China National Chengdu Center for Safety Evaluation of Drugs, Chengdu, China.
authors.forename: T
authors.initials: T
authors.lastname: Chen
authors.affiliation: West China School of Medicine, West China Hospital, Sichuan University, Chengdu, China National Chengdu Center for Safety Evaluation of Drugs, Chengdu, China.
authors.forename: L
authors.initials: L
authors.lastname: Wang
authors.affiliation: West China School of Medicine, West China Hospital, Sichuan University, Chengdu, China National Chengdu Center for Safety Evaluation of Drugs, Chengdu, China.
authors.forename: K
authors.initials: K
authors.lastname: Chen
authors.affiliation: National Chengdu Center for Safety Evaluation of Drugs, Chengdu, China.
authors.forename: S
authors.initials: S
authors.lastname: Qiu
authors.affiliation: West China School of Medicine, West China Hospital, Sichuan University, Chengdu, China National Chengdu Center for Safety Evaluation of Drugs, Chengdu, China.
authors.forename: X
authors.initials: X
authors.lastname: Cen
authors.affiliation: West China School of Medicine, West China Hospital, Sichuan University, Chengdu, China National Chengdu Center for Safety Evaluation of Drugs, Chengdu, China.
authors.forename: H
authors.initials: H
authors.lastname: Li
authors.affiliation: West China School of Medicine, West China Hospital, Sichuan University, Chengdu, China National Chengdu Center for Safety Evaluation of Drugs, Chengdu, China huc2006@hotmail.com.
authors.forename: C
authors.initials: C
authors.lastname: Hu
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D005260:Female; D005720:Gamma Rays / Q000009:adverse effects*; D041981:Gastrointestinal Tract / Q000473:pathology / Q000528:radiation effects*; D007150:Immunohistochemistry; D008253:Macaca mulatta; D008297:Male; D009928:Organ Specificity
keywords: BrdU; Radiation injury; gastrointestinal tract; proliferative activity; rhesus monkey