title: Structural characterization and antioxidant activity in vitro of polysaccharides from angelica and astragalus.
abstract: In the present study, structural characterization and antioxidant activity of a fraction (AAP-2A) of polysaccharides from angelica and astragalus (AAP) were investigated. Characteriztion assay showed that AAP-2A had molecular weight (Mw), root-mean square (RMS) radius and polydispersity index (Mw/Mn) of 2.252 × 10(3)kDa, 28.4 nm and 1.038, respectively. There were infrared characteristic absorption peaks of polysaccharides in FT-IR spectroscopy. AAP-2A was composed of rhamnose (Rha), galactose (Gal), arabinose (Ara) and glucose (Glc) with a molar ratio of 1:2.13:3.22:6.18 in GC analysis. Methylation analysis combined with NMR spectroscopic analysis demonstrated that a preliminary structure of AAP-2A was proposed as follows: 1,3-linked Rhap, 1,3-linked Galp, 1,3-linked Araf, 1,5-linked Araf, 1,3,5-linked Araf, 1,4-linked Glcp and 1,4,6-linked Glcp interspersed with terminal Glcp. AAP-2A exhibited a surface with a sheet-like appearance in scanning electron microscope and stronger antioxidant capacity compared with AAP.
authors.affiliation: College of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730050, China; The Key Lab of Screening, Evaluated and Advanced Processing of Traditional Chinese Medicine and Tibetan Medicine, Gansu Educational Department, Lanzhou 730050, China. Electronic address: 13919262876@163.com.
authors.forename: Xiuying
authors.initials: X
authors.lastname: Pu
authors.affiliation: College of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730050, China; The Key Lab of Screening, Evaluated and Advanced Processing of Traditional Chinese Medicine and Tibetan Medicine, Gansu Educational Department, Lanzhou 730050, China.
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Ma
authors.affiliation: College of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730050, China; The Key Lab of Screening, Evaluated and Advanced Processing of Traditional Chinese Medicine and Tibetan Medicine, Gansu Educational Department, Lanzhou 730050, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Liu
authors.affiliation: College of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730050, China; The Key Lab of Screening, Evaluated and Advanced Processing of Traditional Chinese Medicine and Tibetan Medicine, Gansu Educational Department, Lanzhou 730050, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Ren
authors.affiliation: College of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730050, China; The Key Lab of Screening, Evaluated and Advanced Processing of Traditional Chinese Medicine and Tibetan Medicine, Gansu Educational Department, Lanzhou 730050, China.
authors.forename: Haibing
authors.initials: H
authors.lastname: Li
authors.affiliation: College of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730050, China; The Key Lab of Screening, Evaluated and Advanced Processing of Traditional Chinese Medicine and Tibetan Medicine, Gansu Educational Department, Lanzhou 730050, China.
authors.forename: Xiaoyue
authors.initials: X
authors.lastname: Li
authors.affiliation: College of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730050, China; The Key Lab of Screening, Evaluated and Advanced Processing of Traditional Chinese Medicine and Tibetan Medicine, Gansu Educational Department, Lanzhou 730050, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Yu
authors.affiliation: College of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730050, China; The Key Lab of Screening, Evaluated and Advanced Processing of Traditional Chinese Medicine and Tibetan Medicine, Gansu Educational Department, Lanzhou 730050, China.
authors.forename: Weijie
authors.initials: W
authors.lastname: Zhang
authors.affiliation: College of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730050, China; The Key Lab of Screening, Evaluated and Advanced Processing of Traditional Chinese Medicine and Tibetan Medicine, Gansu Educational Department, Lanzhou 730050, China.
authors.forename: Wenbo
authors.initials: W
authors.lastname: Fan
mesh_terms: D029969:Angelica / Q000737:chemistry*; D000975:Antioxidants / Q000737:chemistry*; D027883:Astragalus Plant / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D011134:Polysaccharides
keywords: Angelica and astragalus; Antioxidant activity; Characterization; Polysaccharides; Purification