title: Impact of reactive oxygen species on antioxidant capacity of male reproductive system.
abstract: The present research work was aimed to study the mutual interaction of reactive oxygen species (ROS) and basal cells antioxidant capacity in the male reproductive system and to further establish the association between selected heavy metals and stress markers. Total oxidant status (TOS) and total antioxidant status (TAS) of serum and seminal plasma were determined by automated photometric methods. The concentrations of Selenium (Se), Lead (Pb), and Cadmium (Cd) were determined by using atomic absorption spectrophotometer. The TOS was increased significantly (P <0.05) in seminal plasma as well as in the serum of the infertile group when compared with the fertile group. On the other hand, the TAS of the infertile group was found to be noticeably decreased (P <0.05) when compared with the TAS of the fertile group. Among the heavy metals, a noticeably lower concentration of Se was detected in the infertile group whereas markedly elevated levels of Cd and Pb were observed in the infertile group compared with the fertile group. Among the infertile group a significant inverse correlation (r = -0.521, P <0.05) was observed between Se and TOS and between Cd and Pb (r = -0.407, P <0.05). Contrarily among the infertile group a considerable positive relationship was established between Se and TAS (r = 0.507, P <0.05). It was concluded that the oxidant stress reduces the antioxidant activity in infertile men by elevating the production of ROS. A lower concentration of Se and elevated levels of Pb and Cd explain the individual's exposure to these heavy metals. The study also revealed that the heavy metal toxicity contributes significantly to male infertility.
authors.affiliation: Department of Biochemistry, Faculty of Sciences, University of Agriculture, Faisalabad, Pakistan.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Riaz
authors.affiliation: Department of Applied Chemistry and Biochemistry, Government College University, Faisalabad, Pakistan.
authors.forename: Zahed
authors.initials: Z
authors.lastname: Mahmood
authors.affiliation: Department of Biochemistry, Faculty of Sciences, University of Agriculture, Faisalabad, Pakistan.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Shahid
authors.affiliation: Depatment of Pathology, Civil Hospital, Bahawalpur, Pakistan.
authors.forename: M Usman Qamar
authors.initials: MU
authors.lastname: Saeed
authors.affiliation: College of Allied Health Professionals, Directorate of Medical Sciences, Government College University, Faisalabad, Pakistan.
authors.forename: Imtiaz Mahmood
authors.initials: IM
authors.lastname: Tahir
authors.affiliation: Department of Eastern Medicine, Government College University, Faisalabad, Pakistan smalishah@hotmail.com.
authors.forename: Sm Ali
authors.initials: SA
authors.lastname: Shah
authors.affiliation: Department of Biochemistry, Faculty of Sciences, University of Agriculture, Faisalabad, Pakistan.
authors.forename: Naveed
authors.initials: N
authors.lastname: Munir
authors.affiliation: Department of Chemistry, Faculty of Science, Aljouf University, Kingdom of Saudi Arabia.
authors.forename: Ahmed
authors.initials: A
authors.lastname: El-Ghorab
mesh_terms: D000975:Antioxidants / Q000378:metabolism*; D000075322:Heavy Metal Poisoning; D006801:Humans; D007248:Infertility, Male / Q000378:metabolism; D008297:Male; D019216:Metals, Heavy / Q000009:adverse effects; D011041:Poisoning; D017382:Reactive Oxygen Species / Q000378:metabolism*; D012098:Reproduction / Q000502:physiology*; D012661:Semen / Q000187:drug effects / Q000378:metabolism; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects / Q000378:metabolism
chemical_list: D000975:Antioxidants; D019216:Metals, Heavy; D017382:Reactive Oxygen Species
keywords: Lead and Cadmium; Selenium; antioxidants; infertility; reactive oxygen species