title: Oncogenic role of the TP53-induced glycolysis and apoptosis regulator in nasopharyngeal carcinoma through NF-κB pathway modulation.
abstract: The TP53-induced glycolysis and apoptosis regulator (TIGAR) is a p53 target gene, which functions to suppress reactive oxygen species (ROS) damage and protect cells from apoptosis. In this study, we investigated the role of TIGAR in nasopharyngeal carcinoma (NPC) tumorigenesis. Imnunohistochemical analysis of the tissue specimens from nasopharyngeal carcinoma patients showed a higher expression level of TIGAR in tumor tissues, compared with normal nasopharyngeal epithelium. Knockdown of TIGAR by lentivirus-shRNA in CNE-2 or 5-8F cells resulted in decreased cell growth, colony formation, migration, invasion, and induced apoptosis. TIGAR overexpression exerted the opposite effects except for apoptosis reduction. In the xenograft tumor models, TIGAR knockdown reduced tumor growth rate and weight, whereas TIGAR overexpression showed the opposite effects. In addition, the NF-κB signaling pathway was decreased in TIGAR silenced cells. In conclusion, our data demonstrated that TIGAR acted as an oncogene in NPC tumorigenesis, and knockdown of TIGAR inhibited NPC tumor growth through the NF-κB pathway.
authors.affiliation: Department of Oncology, The First Hospital of Sichuan Medical University, Luzhou, Sichuan 646000, P.R. China.
authors.forename: Ming
authors.initials: M
authors.lastname: Zhao
authors.affiliation: Department of Oncology, The First Hospital of Sichuan Medical University, Luzhou, Sichuan 646000, P.R. China.
authors.forename: Juan
authors.initials: J
authors.lastname: Fan
authors.affiliation: Department of Oncology, The First Hospital of Sichuan Medical University, Luzhou, Sichuan 646000, P.R. China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Oncology, The First Hospital of Sichuan Medical University, Luzhou, Sichuan 646000, P.R. China.
authors.forename: Yanxin
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Department of Oncology, The First Hospital of Sichuan Medical University, Luzhou, Sichuan 646000, P.R. China.
authors.forename: Jinhui
authors.initials: J
authors.lastname: Xu
authors.affiliation: Department of Oncology, The First Hospital of Sichuan Medical University, Luzhou, Sichuan 646000, P.R. China.
authors.forename: Qinglian
authors.initials: Q
authors.lastname: Wen
authors.affiliation: Department of Oncology, The First Hospital of Sichuan Medical University, Luzhou, Sichuan 646000, P.R. China.
authors.forename: Jianwen
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Oncology, The First Hospital of Sichuan Medical University, Luzhou, Sichuan 646000, P.R. China.
authors.forename: Shaozhi
authors.initials: S
authors.lastname: Fu
authors.affiliation: Department of Oncology, The First Hospital of Sichuan Medical University, Luzhou, Sichuan 646000, P.R. China.
authors.forename: Biqiong
authors.initials: B
authors.lastname: Wang
authors.affiliation: Department of Oncology, The First Hospital of Sichuan Medical University, Luzhou, Sichuan 646000, P.R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Xiang
authors.affiliation: Department of Oncology, The First Hospital of Sichuan Medical University, Luzhou, Sichuan 646000, P.R. China.
authors.forename: Jing
authors.initials: J
authors.lastname: Feng
authors.affiliation: Department of Oncology, The First Hospital of Sichuan Medical University, Luzhou, Sichuan 646000, P.R. China.
authors.forename: Jingbo
authors.initials: J
authors.lastname: Wu
authors.affiliation: Department of Oncology, The First Hospital of Sichuan Medical University, Luzhou, Sichuan 646000, P.R. China.
authors.forename: Linglin
authors.initials: L
authors.lastname: Yang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism*; D002277:Carcinoma; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation / Q000502:physiology; D006019:Glycolysis / Q000502:physiology*; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D016328:NF-kappa B / Q000378:metabolism*; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000378:metabolism*; D010744:Phosphoric Monoester Hydrolases; D034741:RNA, Small Interfering / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
chemical_list: D051017:Apoptosis Regulatory Proteins; D047908:Intracellular Signaling Peptides and Proteins; D016328:NF-kappa B; D034741:RNA, Small Interfering; D017382:Reactive Oxygen Species; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; D010744:Phosphoric Monoester Hydrolases; C512708:TIGAR protein, human