title: Genetic Characterization of Toxoplasma gondii Isolates from Pigs in Jilin Province, Northeastern China.
abstract: Toxoplasma gondii is prevalent in humans and animals worldwide. The present study aimed to determine the genetic diversity of T. gondii isolates from pigs in Jilin province, northeastern China. A total of 100 DNA samples were extracted from the hilar lymph nodes of slaughtered pigs, and 9 (9.0%, 95% confidence interval: 3.4-14.6%) were detected positive for T. gondii B1 gene by a nested polymerase chain reaction (PCR). The positive DNA samples were typed at 11 genetic markers, including 10 nuclear loci (SAG1, 5'-SAG2, and 3'-SAG2, alternative SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, and PK1) and an apicoplast locus (Apico) using the multilocus PCR-restriction fragment length polymorphism technology. Only three isolates were completely typed at all loci, showing that they all belonged to the clonal type I. One isolate was typed at five loci, including 5' +3'-SAG2, SAG2, SAG3, GRA6, and L358, revealing the possible clonal type I. This is the first report of the genetic characterization of T. gondii isolates in pigs in Jilin province, northeastern China, which has implications for better understanding the population structure of T. gondii infection in China.
authors.affiliation: 1 State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute , Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province, China .|2 College of Veterinary Medicine, China Agricultural University , Beijing, China .
authors.forename: Hai-Hai
authors.initials: HH
authors.lastname: Jiang
authors.affiliation: 3 Military Veterinary Institute , Academy of Military Medical Sciences, Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun, Jilin Province, China .
authors.forename: Shu-Chao
authors.initials: SC
authors.lastname: Wang
authors.affiliation: 1 State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute , Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province, China .|4 Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses , Yangzhou, Jiangsu Province, China .
authors.forename: Si-Yang
authors.initials: SY
authors.lastname: Huang
authors.affiliation: 1 State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute , Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province, China .
authors.forename: Lei
authors.initials: L
authors.lastname: Zhao
authors.affiliation: 3 Military Veterinary Institute , Academy of Military Medical Sciences, Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun, Jilin Province, China .
authors.forename: Ze-Dong
authors.initials: ZD
authors.lastname: Wang
authors.affiliation: 1 State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute , Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province, China .|4 Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses , Yangzhou, Jiangsu Province, China .
authors.forename: Xing-Quan
authors.initials: XQ
authors.lastname: Zhu
authors.affiliation: 3 Military Veterinary Institute , Academy of Military Medical Sciences, Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun, Jilin Province, China .
authors.forename: Quan
authors.initials: Q
authors.lastname: Liu
mesh_terms: D000003:Abattoirs; D000818:Animals; D002681:China / Q000453:epidemiology; D016054:DNA, Protozoan / Q000032:analysis*; D005819:Genetic Markers; D014644:Genetic Variation; D008198:Lymph Nodes / Q000469:parasitology; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D015995:Prevalence; D013552:Swine / Q000382:microbiology*; D013553:Swine Diseases / Q000453:epidemiology / Q000469:parasitology; D014122:Toxoplasma / Q000235:genetics* / Q000302:isolation & purification; D014124:Toxoplasmosis, Animal / Q000453:epidemiology / Q000469:parasitology
chemical_list: D016054:DNA, Protozoan; D005819:Genetic Markers