title: Knocking down Dp71 expression in A549 cells reduces its malignancy in vivo and in vitro.
abstract: Dp71 is one of the most ubiquitously expressed isoforms of dystrophin, the pathological genes of DMD. In order to find whether the alteration of Dp71 can affect the phenotypes of cell other than PC12, an A549 cell line with stably transfected Dp71 siRNA plasmids was set up and named A549-Dp71AS cell. It is demonstrated for the first time that the A549-Dp71AS cell line displayed decreased invasion capabilities, reduced migration ability, decreased proliferation rate, and lessened clonogenic formation. Cisplatin-induced apoptosis was also increased in A549-Dp71AS cell line via enhancing the Caspase 3, Caspase 8, and Caspase 9 activities. Knocking down Dp71 expression can significantly inhibit the A549 xenograft tumor growth in nude mice. The A549-Dp71AS cells and xenograft tumor tissues displayed reduced lamin B1, Bcl-2, and MMP2 protein expression, which accounts for the reduced malignancy of A549-Dp71AS cells in vivo and in vitro.
authors.affiliation: a Department of Thoracic Surgery, Second Xiangya Hospital , Central South University , Changsha , Hunan , China.
authors.forename: Sichuang
authors.initials: S
authors.lastname: Tan
authors.affiliation: b Laboratory of Shock, Department of Pathophysiology, Xiangya School of Medicine , Central South University , Hunan , China.|c Molecular and Cell Experimental Center, Xiangya School of Medicine , Central South University , Changsha , Hunan , China.
authors.forename: Sipin
authors.initials: S
authors.lastname: Tan
authors.affiliation: d Department of General Surgery, Xiangya Hospital , Central South University , Hunan , China.
authors.forename: Zhikang
authors.initials: Z
authors.lastname: Chen
authors.affiliation: e Center of Transplant Surgery, Third Xiangya Hospital , Central South University , Hunan , China.
authors.forename: Ke
authors.initials: K
authors.lastname: Cheng
authors.affiliation: e Center of Transplant Surgery, Third Xiangya Hospital , Central South University , Hunan , China.
authors.forename: Zhicao
authors.initials: Z
authors.lastname: Chen
authors.affiliation: b Laboratory of Shock, Department of Pathophysiology, Xiangya School of Medicine , Central South University , Hunan , China.
authors.forename: Wenmei
authors.initials: W
authors.lastname: Wang
authors.affiliation: d Department of General Surgery, Xiangya Hospital , Central South University , Hunan , China.
authors.forename: Qiaocheng
authors.initials: Q
authors.lastname: Wen
authors.affiliation: d Department of General Surgery, Xiangya Hospital , Central South University , Hunan , China.
authors.forename: Weilin
authors.initials: W
authors.lastname: Zhang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics; D053148:Caspase 3 / Q000378:metabolism; D053181:Caspase 8 / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D004195:Disease Models, Animal; D016189:Dystrophin / Q000172:deficiency* / Q000235:genetics*; D015870:Gene Expression; D055785:Gene Knockdown Techniques; D064593:Heterografts; D006801:Humans; D034921:Lamin Type B / Q000235:genetics / Q000378:metabolism; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism; D060787:Neoplasm Grading; D009369:Neoplasms / Q000235:genetics* / Q000473:pathology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D014410:Tumor Stem Cell Assay
chemical_list: D016189:Dystrophin; D034921:Lamin Type B; D019253:Proto-Oncogene Proteins c-bcl-2; D034741:RNA, Small Interfering; C085339:apo-dystrophin 1; D053148:Caspase 3; D053181:Caspase 8; D053453:Caspase 9; D020778:Matrix Metalloproteinase 2
keywords: A549; Dp71; Knockdown; Malignancy