title: [Morphological evaluation of the optic nerve and retina in the rat model of non-arteritic anterior ischemic optic neuropathy].
abstract: OBJECTIVE
  To evaluate the morphology of optic nerve and retina in the model of the rat non-arteritic anterior ischemic optic neuropathy (rNAION).
  
  
  METHODS
  Experimental study. Thirty-six SD rats were randomly divided into four groups: 6 for naïve group, 3 for laser group, 3 for Rose Bengal (RB) group and 24 for rNAION group. After rNAION was induced by RB and laser, the retina and optic nerve were observed by Hematoxylin-Eosin (HE) staining and the optic nerve was investigated by transmission electron microscope and toludidine blue staining at different time points.
  
  
  RESULTS
  With these methods, significant changes were only found in the RB-laser induced eyes. The damage only occurred in the retinal ganglion cell (RGC) layer without any loss of the other layers of retina in the rNAION. Toludine blue-stained optic nerve showed that there was a loss of axons centrally and largely intact axons were spared in the peripheral nerve. More and more degenerative axons were observed with the transmission electron microscope as time went on.
  
  
  CONCLUSIONS
  The morphological changes of HE staining, toludine blue staining and transmission electron microscopic finding were consistent with each other. The results made a contribution to further study.
authors.affiliation: Department of Ophthalmology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Chen
authors.affiliation: Department of Ophthalmology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Ma
authors.affiliation: Department of Ophthalmology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China.
authors.forename: Yiwei
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Ophthalmology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China.
authors.forename: Donghui
authors.initials: D
authors.lastname: Li
authors.affiliation: Department of Ophthalmology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China; Email: yzhong_eye@163.com.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhong
mesh_terms: D000818:Animals; D001369:Axons / Q000473:pathology* / Q000648:ultrastructure; D004195:Disease Models, Animal; D046529:Microscopy, Electron, Transmission; D009900:Optic Nerve / Q000473:pathology* / Q000648:ultrastructure; D018917:Optic Neuropathy, Ischemic / Q000473:pathology*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012160:Retina / Q000473:pathology* / Q000648:ultrastructure; D012165:Retinal Ganglion Cells / Q000473:pathology* / Q000648:ultrastructure