title: Histology, composition, and quality traits of chicken Pectoralis major muscle affected by wooden breast abnormality.
abstract: Only a few years ago, the poultry industry began to face a recent abnormality in breast meat, known as wooden breast, which frequently overlaps with white striping. This study aimed to assess the impact of wooden breast abnormality on quality traits of meat. For this purpose, 32 normal (NRM), 32 wooden (WB), and 32 wooden and white-striped (WB/WS) Pectoralis major muscles were selected from the same flock of heavy broilers (males, Ross 708, weighing around 3.7 kg) in the deboning area of a commercial processing plant at 3 h postmortem and used to assess histology, proximate (moisture, protein, fat, ash, and collagen) and mineral composition (Mg, K, P, Na and Ca), sarcoplasmic and myofibrillar protein patterns, and technological traits of breast meat. Compared to the normal group, WB/WS fillets showed more severe histological lesions characterized by fiber degeneration, fibrosis, and lipidosis, coupled with a significantly harder texture. With regard to proximate and mineral composition, abnormal samples exhibited significantly (P < 0.001) higher moisture, fat, and collagen contents coupled with lower (P < 0.001) amounts of protein and ash. Furthermore, increased calcium (131 vs. 84 mg kg(-1); P < 0.05) and sodium (741 vs. 393 mg kg(-1); P < 0.001) levels were found in WB/WS meat samples. The SDS-PAGE analysis revealed a significantly lower amount of calcium-ATPase (SERCA, 114 kDa), responsible for the translocation of Ca ions across the membrane, in normal breasts compared to abnormal ones. As for meat quality traits, fillets affected by wooden abnormality exhibited significantly (P < 0.001) higher ultimate pH and lower water-holding/water-binding capacity. In particular, compared to normal, abnormal samples showed reduced marinade uptake coupled with increased drip loss and cooking losses as well. In conclusion, this study revealed that meat affected by wooden breast or both wooden breast and white striping abnormalities exhibit poorer nutritional value, harder texture, and impaired water-holding capacity.
authors.affiliation: Department of Agricultural and Food Sciences, Alma Mater Studiorum - University of Bologna, 47521 Cesena, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Soglia
authors.affiliation: Department of Nutrition and Food Technology, Faculty of Agriculture and Veterinary Medicine, An-Najah National University, P.O. Box 7, Nablus, Palestine.
authors.forename: S
authors.initials: S
authors.lastname: Mudalal
authors.affiliation: Department of Agricultural and Food Sciences, Alma Mater Studiorum - University of Bologna, 47521 Cesena, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Babini
authors.affiliation: Department of Agricultural and Food Sciences, Alma Mater Studiorum - University of Bologna, 47521 Cesena, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Di Nunzio
authors.affiliation: Department of Veterinary Medical Sciences, Alma Mater Studiorum - University of Bologna, 40064 Ozzano dell'Emilia (BO), Italy.
authors.forename: M
authors.initials: M
authors.lastname: Mazzoni
authors.affiliation: Department of Agricultural and Food Sciences, Alma Mater Studiorum - University of Bologna, 47521 Cesena, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Sirri
authors.affiliation: Department of Agricultural and Food Sciences, Alma Mater Studiorum - University of Bologna, 47521 Cesena, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Cavani
authors.affiliation: Department of Agricultural and Food Sciences, Alma Mater Studiorum - University of Bologna, 47521 Cesena, Italy m.petracci@unibo.it.
authors.forename: M
authors.initials: M
authors.lastname: Petracci
mesh_terms: D000818:Animals; D030161:Avian Proteins / Q000378:metabolism*; D002645:Chickens / Q000033:anatomy & histology / Q000502:physiology*; D008297:Male; D008460:Meat / Q000032:analysis*; D008903:Minerals / Q000032:analysis; D009124:Muscle Proteins / Q000378:metabolism*; D010369:Pectoralis Muscles / Q000033:anatomy & histology / Q000737:chemistry / Q000502:physiology*
chemical_list: D030161:Avian Proteins; D008903:Minerals; D009124:Muscle Proteins
keywords: calcium; chicken breast meat; composition; white striping; wooden breast