title: Cryptochrome 1 interacts with PIF4 to regulate high temperature-mediated hypocotyl elongation in response to blue light.
abstract: Cryptochrome 1 (CRY1) is a blue light receptor that mediates primarily blue-light inhibition of hypocotyl elongation. Very little is known of the mechanisms by which CRY1 affects growth. Blue light and temperature are two key environmental signals that profoundly affect plant growth and development, but how these two abiotic factors integrate remains largely unknown. Here, we show that blue light represses high temperature-mediated hypocotyl elongation via CRY1. Furthermore, CRY1 interacts directly with PHYTOCHROME-INTERACTING FACTOR 4 (PIF4) in a blue light-dependent manner to repress the transcription activity of PIF4. CRY1 represses auxin biosynthesis in response to elevated temperature through PIF4. Our results indicate that CRY1 signal by modulating PIF4 activity, and that multiple plant photoreceptors [CRY1 and PHYTOCHROME B (PHYB)] and ambient temperature can mediate morphological responses through the same signaling component-PIF4.
authors.affiliation: National Key Laboratory of Plant Molecular Genetics, Chinese Academy of Sciences Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China;
authors.forename: Dingbang
authors.initials: D
authors.lastname: Ma
authors.affiliation: National Key Laboratory of Plant Molecular Genetics, Chinese Academy of Sciences Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China;
authors.forename: Xu
authors.initials: X
authors.lastname: Li
authors.affiliation: Jack H. Skirball Center for Chemical Biology and Proteomics, Salk Institute for Biological Studies, La Jolla, CA 92037;
authors.forename: Yongxia
authors.initials: Y
authors.lastname: Guo
authors.affiliation: National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China;
authors.forename: Jingfang
authors.initials: J
authors.lastname: Chu
authors.affiliation: National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China;
authors.forename: Shuang
authors.initials: S
authors.lastname: Fang
authors.affiliation: National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China;
authors.forename: Cunyu
authors.initials: C
authors.lastname: Yan
authors.affiliation: Jack H. Skirball Center for Chemical Biology and Proteomics, Salk Institute for Biological Studies, La Jolla, CA 92037; Howard Hughes Medical Institute, The Salk Institute for Biological Studies, La Jolla, CA 92037.
authors.forename: Joseph P
authors.initials: JP
authors.lastname: Noel
authors.affiliation: National Key Laboratory of Plant Molecular Genetics, Chinese Academy of Sciences Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China; htliu@sibs.ac.cn.
authors.forename: Hongtao
authors.initials: H
authors.lastname: Liu
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development* / Q000528:radiation effects; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D051792:Basic Helix-Loop-Helix Proteins / Q000378:metabolism*; D056931:Cryptochromes / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D006358:Hot Temperature; D018546:Hypocotyl / Q000235:genetics / Q000254:growth & development* / Q000528:radiation effects; D007210:Indoleacetic Acids / Q000378:metabolism; D008027:Light; D006899:Mixed Function Oxygenases / Q000235:genetics; D051939:Phytochrome B / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D029681:Arabidopsis Proteins; D051792:Basic Helix-Loop-Helix Proteins; D056931:Cryptochromes; D007210:Indoleacetic Acids; D006899:Mixed Function Oxygenases; D051939:Phytochrome B; C538962:CRY1 protein, Arabidopsis; C497952:PHYB protein, Arabidopsis; C459466:PIF4 protein, Arabidopsis; C585335:YUCCA8 protein, Arabidopsis
keywords: PIF4; ambient temperature; blue light; cryptochrome; hypocotyl elongation