title: A novel method for a fast diagnosis of septic arthritis using mid infrared and deported spectroscopy.
abstract: OBJECTIVE
  To assess the ability of mid infrared deported spectroscopy to discriminate synovial fluids samples of septic arthritis patients from other causes of joint effusion.
  
  
  METHODS
  Synovial fluids obtained from patients with clinically suspected arthritis were collected, analysed and classified according to standard diagnostic procedures as septic or non-septic. A spectroscopic analysis on synovial fluid samples was then performed using a coiled optical fiber made with chalcogenide glass. After a factorial analysis of the normalized spectra and the computation of a Fisher test used to select the most relevant components, a logistic regression model was fitted, allowing to attribute a score between 0 - non-septic -, and 1 - septic.
  
  
  RESULTS
  In a first phase, we analysed the synovial fluids from 122 different synovial fluids including 6 septic arthritis among arthritis of various origins. Septic synovial fluids were identified with a sensitivity of 95.8% and a specificity of 93.9% and an AUROC of 0.977. The analysis of an independent set of samples (n=42, including two septic arthritis) gave similar values.
  
  
  CONCLUSIONS
  Our data strongly supports the interest of mid infrared deported spectroscopy, which could be used potentially at point of care, for a rapid and easy diagnosis of septic arthritis. Now, the precision of the diagnosis must be evaluated through a multicentric study on a larger panel of patients.
authors.affiliation: Department of Rheumatology, CHU of Rennes, 2, rue Henri-le-Guilloux, 35000 Rennes, France; INSERM UMR 991, 35033 Rennes, France. Electronic address: jean-david.albert@chu-rennes.fr.
authors.forename: Jean-David
authors.initials: JD
authors.lastname: Albert
authors.affiliation: University of Rennes 1, 35043 Rennes, France; UMR-CNRS 6625, IRMAR Mathematics Research Institute of Rennes, 35033 Rennes, France; INRIA/ASPI, 35033 Rennes, France.
authors.forename: Valérie
authors.initials: V
authors.lastname: Monbet
authors.affiliation: Pole of Biology, EA 1254 Microbiologie, CHU of Rennes, University of Rennes 1, 35033 Rennes, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Jolivet-Gougeon
authors.affiliation: Diafir™, avenue Chardonnet, Parc Lorans, 26J, 35700 Rennes, France.
authors.forename: Nadia
authors.initials: N
authors.lastname: Fatih
authors.affiliation: Diafir™, avenue Chardonnet, Parc Lorans, 26J, 35700 Rennes, France; Laboratoire d'Ingénierie des Matériaux de Bretagne (LIMATB), University of Bretagne-Sud, CER Yves-Coppens, BP 573, 56017 Vannes cedex, France.
authors.forename: Maëna
authors.initials: M
authors.lastname: Le Corvec
authors.affiliation: University of Rennes 1, 35043 Rennes, France.
authors.forename: Malik
authors.initials: M
authors.lastname: Seck
authors.affiliation: Diafir™, avenue Chardonnet, Parc Lorans, 26J, 35700 Rennes, France.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Charpentier
authors.affiliation: Department of Rheumatology, CHU of Rennes, 2, rue Henri-le-Guilloux, 35000 Rennes, France; INSERM UMR 991, 35033 Rennes, France.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Coiffier
authors.affiliation: ISCR UMR-CNRS 6226, Verres et Céramiques, Université de Rennes 1, 35042 Rennes, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Boussard-Pledel
authors.affiliation: ISCR UMR-CNRS 6226, Verres et Céramiques, Université de Rennes 1, 35042 Rennes, France.
authors.forename: Bruno
authors.initials: B
authors.lastname: Bureau
authors.affiliation: Department of Rheumatology, CHU of Rennes, 2, rue Henri-le-Guilloux, 35000 Rennes, France; INSERM UMR 991, 35033 Rennes, France; University of Rennes 1, 35043 Rennes, France.
authors.forename: Pascal
authors.initials: P
authors.lastname: Guggenbuhl
authors.affiliation: INSERM UMR 991, 35033 Rennes, France; University of Rennes 1, 35043 Rennes, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Loréal
mesh_terms: D001170:Arthritis, Infectious / Q000175:diagnosis*; D003430:Cross-Sectional Studies; D006801:Humans; D007592:Joint Diseases / Q000175:diagnosis; D013057:Spectrum Analysis / Q000379:methods*; D013582:Synovial Fluid / Q000737:chemistry*
keywords: Arthritis; Deported mid infrared spectroscopy; Diagnosis; Infectious; Synovial fluid