title: Gastro protective and H(+), K(+)-ATPase/H. pylori inhibitory properties of pectic polysaccharides from potato.
abstract: Polysaccharide is one among the important classes of biological polymers that is reported to exhibit disease preventive properties. The present study describes the isolation of galactans and confirmation of the same by sugar analysis and determination of anti-ulcer effect of Potato Galactan Polysaccharide (PGP). Data indicated that PGP possessed sugar composition of rhamnose (2%), arabinose (3%), mannose (3%), galactose (94%) and uronic acid (17%) confirming that PGP thus isolated is a galactan. PGP exhibited potent H(+), K(+)-ATPase inhibitory activity (IC50 420 μg/mL) in vitro as opposed to lansoprazole, a known antiulcer drug with IC50 19.3 μg/mL. The antiulcer potency of PGP was evaluated in ethanol stress induced gastric ulcer model in vivo. About 84% reduction in ulcer index; enhanced mucosal recovery, normalization of H(+), K(+)-ATPase, antioxidant and antioxidant enzymes substantiated the antiulcer potentials of PGP. Mucosal recovery could be attributed to cytoprotective and DNA protective ability of PGP that can help in mucosal layer regeneration. Further, PGP was also effective in inhibiting Helicobacter pylori as per growth inhibition assay followed by scanning electron microscopic studies suggesting that PGP is effective in curbing the growth of H. pylori, which is responsible for ∼70% of gastric ulcer/cancer incidences.
authors.affiliation: Department of Biochemistry and Nutrition, CSIR-Central Food Technological Research Institute, Mysuru 570 020, Karnataka, India.
authors.forename: Kavitha
authors.initials: K
authors.lastname: Badanavalu Chandrashekar
authors.affiliation: Department of Biochemistry and Nutrition, CSIR-Central Food Technological Research Institute, Mysuru 570 020, Karnataka, India. Electronic address: shylaja@cftri.res.in.
authors.forename: Shylaja Mallaiah
authors.initials: SM
authors.lastname: Dharmesh
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology*; D000897:Anti-Ulcer Agents / Q000737:chemistry / Q000494:pharmacology; D002241:Carbohydrates / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D004195:Disease Models, Animal; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology; D005752:Gastric Mucins / Q000037:antagonists & inhibitors; D005753:Gastric Mucosa / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D017506:H(+)-K(+)-Exchanging ATPase / Q000378:metabolism; D016480:Helicobacter pylori / Q000187:drug effects / Q000648:ultrastructure; D020128:Inhibitory Concentration 50; D008826:Microbial Sensitivity Tests; D008970:Molecular Weight; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology*; D054328:Proton Pump Inhibitors / Q000737:chemistry* / Q000494:pharmacology*; D051381:Rats; D011198:Solanum tuberosum / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013276:Stomach Ulcer / Q000188:drug therapy / Q000209:etiology / Q000473:pathology
chemical_list: D000900:Anti-Bacterial Agents; D000897:Anti-Ulcer Agents; D002241:Carbohydrates; D016166:Free Radical Scavengers; D005752:Gastric Mucins; D011134:Polysaccharides; D054328:Proton Pump Inhibitors; D017506:H(+)-K(+)-Exchanging ATPase
keywords: Gastric ulcer; Helicobacter pylori; Solanum tuberosum