title: Storage of Heparinised Canine Whole Blood for the Measurement of Glutathione Peroxidase Activity.
abstract: Glutathione peroxidase activity is used as a biomarker of selenium status in dogs. Freshly collected blood samples are usually measured, due to the lack of knowledge on the effect of storing the samples. This study investigated if the analysis of glutathione peroxidase activity in whole blood collected from dogs was affected by storage of between 5 and 164 days. Results indicated that glutathione peroxidase activity was more variable in the freshly analysed samples compared to the stored samples. Although the mean differences between fresh and stored samples were not always equal to zero, this is thought to be caused by the variability of reagent preparation rather than by storage, as no consistent increase or decrease in glutathione peroxidase activity was found. Therefore, it can be concluded that heparinised dog blood samples can be successfully stored up to 164 days before analysis of glutathione peroxidase activity.
authors.affiliation: Laboratory of Animal Nutrition, Faculty of Veterinary Medicine, Ghent University, Heidestraat 19, Merelbeke, Belgium.
authors.forename: Mariëlle
authors.initials: M
authors.lastname: van Zelst
authors.affiliation: Laboratory of Animal Nutrition, Faculty of Veterinary Medicine, Ghent University, Heidestraat 19, Merelbeke, Belgium.
authors.forename: Myriam
authors.initials: M
authors.lastname: Hesta
authors.affiliation: WALTHAM Centre for Pet Nutrition, Freeby Lane, Waltham-on-the-Wolds, Leicestershire, UK.
authors.forename: Kerry
authors.initials: K
authors.lastname: Gray
authors.affiliation: Laboratory of Animal Nutrition, Faculty of Veterinary Medicine, Ghent University, Heidestraat 19, Merelbeke, Belgium. Nutrition@UGent.be.
authors.forename: Geert P J
authors.initials: GPJ
authors.lastname: Janssens
mesh_terms: D000818:Animals; D055162:Biocatalysis; D001800:Blood Specimen Collection; D004285:Dogs; D005979:Glutathione Peroxidase / Q000097:blood* / Q000378:metabolism*
chemical_list: D005979:Glutathione Peroxidase
keywords: Antioxidant; Canine; Freeze; GPx; Selenium; Storage