title: Antifatigue Activity of Liquid Cultured Tricholoma matsutake Mycelium Partially via Regulation of Antioxidant Pathway in Mouse.
abstract: Tricholoma matsutake has been popular as food and biopharmaceutical materials in Asian countries for its various pharmacological activities. The present study aims to analyze the antifatigue effects on enhancing exercise performance of Tricholoma matsutake fruit body (ABM) and liquid cultured mycelia (TM) in mouse model. Two-week Tricholoma matsutake treatment significantly enhances the exercise performance in weight-loaded swimming, rotating rod, and forced running test. In TM- and ABM-treated mice, some factors were observed at 60 min after swimming compared with nontreated mice, such as the increased levels of adenosine triphosphate (ATP), antioxidative enzymes, and glycogen and the reduced levels of malondialdehyde and reactive oxygen species in muscle, liver, and/or serum. Further data obtained from western blot show that CM and ABM have strongly enhanced the activation of 5'-AMP-activated protein kinase (AMPK), and the expressions of peroxisome proliferator have activated receptor γ coactivator-1α (PGC-1α) and phosphofructokinase-1 (PFK-1) in liver. Our data suggest that both Tricholoma matsutake fruit body and liquid cultured mycelia possess antifatigue effects related to AMPK-linked antioxidative pathway. The information uncovered in our study may serve as a valuable resource for further identification and provide experimental evidence for clinical trials of Tricholoma matsutake as an effective agent against fatigue related diseases.
authors.affiliation: School of Life Sciences, Jilin University, Changchun 130012, China.
authors.forename: Quan
authors.initials: Q
authors.lastname: Li
authors.affiliation: School of Life Sciences, Jilin University, Changchun 130012, China ; Zhuhai College of Jilin University, Jilin University, Guangzhou 519041, China.
authors.forename: Yanzhen
authors.initials: Y
authors.lastname: Wang
authors.affiliation: School of Life Sciences, Jilin University, Changchun 130012, China.
authors.forename: Guangsheng
authors.initials: G
authors.lastname: Cai
authors.affiliation: School of Life Sciences, Jilin University, Changchun 130012, China.
authors.forename: Fange
authors.initials: F
authors.lastname: Kong
authors.affiliation: School of Life Sciences, Jilin University, Changchun 130012, China.
authors.forename: Xiaohan
authors.initials: X
authors.lastname: Wang
authors.affiliation: School of Life Sciences, Jilin University, Changchun 130012, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: School of Chinese Medicine, Hong Kong Baptist University, Kowloon, Hong Kong.
authors.forename: Chuanbin
authors.initials: C
authors.lastname: Yang
authors.affiliation: School of Life Sciences, Jilin University, Changchun 130012, China.
authors.forename: Di
authors.initials: D
authors.lastname: Wang
authors.affiliation: School of Life Sciences, Jilin University, Changchun 130012, China ; Zhuhai College of Jilin University, Jilin University, Guangzhou 519041, China.
authors.forename: Lirong
authors.initials: L
authors.lastname: Teng
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000096:biosynthesis; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage* / Q000737:chemistry; D003470:Culture Media / Q000494:pharmacology; D005221:Fatigue / Q000188:drug therapy* / Q000503:physiopathology; D005786:Gene Expression Regulation / Q000187:drug effects; D006003:Glycogen / Q000378:metabolism; D006801:Humans; D051379:Mice; D025282:Mycelium / Q000737:chemistry; D010805:Physical Conditioning, Animal / Q000502:physiology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013550:Swimming; D055439:Tricholoma / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D003470:Culture Media; D017382:Reactive Oxygen Species; D006003:Glycogen; D055372:AMP-Activated Protein Kinases