title: Determination of work of adhesion of biological cell under AFM bead indentation.
abstract: Hertz contact theory has been widely used for the determination of cell elasticity based on AFM indentation experiments. In light of the adhesive contact between AFM tip and cell, this study applied Johnson-Kendall-Roberts (JKR) model to fit the indentation force-displacement (F-D) curves reported previously. A MIN6 cell has been modeled as first a sphere and then a flattened cell with different thicknesses. The results have shown that both basic JKR model and "generalized" JKR model can best describe the unloading force-displacement behaviors of the indentation curves. The Young׳s modulus of the cell and the work of adhesion of the cell-indenter interface are obtained. In comparison to the Hertzian contact model, the JKR model provides obviously better fitting to the experimental results, indicating that the adhesion is significant in the cell interaction.
authors.affiliation: School of Engineering, University of Warwick, Coventry CV4 7AL, UK.
authors.forename: Xinyao
authors.initials: X
authors.lastname: Zhu
authors.affiliation: School of Engineering, University of Warwick, Coventry CV4 7AL, UK.
authors.forename: E
authors.initials: E
authors.lastname: Siamantouras
authors.affiliation: School of Engineering, University of Warwick, Coventry CV4 7AL, UK.
authors.forename: K K
authors.initials: KK
authors.lastname: Liu
authors.affiliation: School of Engineering, University of Warwick, Coventry CV4 7AL, UK. Electronic address: X.Liu@warwick.ac.uk.
authors.forename: X
authors.initials: X
authors.lastname: Liu
mesh_terms: D001696:Biomechanical Phenomena; D002448:Cell Adhesion; D002460:Cell Line; D055119:Elastic Modulus; D008422:Materials Testing; D055595:Mechanical Phenomena; D018625:Microscopy, Atomic Force; D008863:Microspheres; D008954:Models, Biological; D014783:Viscosity
keywords: AFM indentation; JKR model; Work of adhesion; Young׳s modulus