title: Burkholderia jirisanensissp. nov., isolated from forest soil.
abstract: A Gram-negative, catalase-positive, mesophilic, obligately aerobic bacterium designated JRM2-1Twas isolated from forest soil of Jirisan Mountain, Republic of Korea, and its taxonomic position was investigated based on a polyphasic taxonomic approach. Cells of strain JRM2-1Tgrew optimally at pH 5.0-7.0 and at 25 °C. Strain JRM2-1Twas susceptible to chloramphenicol, gentamicin, kanamycin, nalidixic acid, rifampicin, streptomycin and tetracycline. On the basis of 16S rRNA gene sequence similarity, the closest neighbour of strain JRM2-1TwasBurkholderia rhizosphaeraeWR43T(98.1 %). On the basis of our phylogenetic analysis, strain JRM2-1Tis clearly distinguished from related species of the genusBurkholderiaand is clustered with plant-associated members of the genus. The major cellular fatty acids were C16 : 0, C17 : 0cyclo and C19 : 0cyclo ω8c. The polar lipid profile of strain JRM2-1Tcontained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, several unidentified aminolipids and an unidentified aminophospholipid. The isoprenoid quinone of strain JRM2-1Twas Q-8 and the DNA G+C content was 63.7 mol%. On the basis of our polyphasic taxonomic investigation, strain JRM2-1Tis considered to represent a novel species in the genusBurkholderia, for which the nameBurkholderia jirisanensissp. nov. is proposed. The type strain is JRM2-1T( = AIM 0373T= KCTC 42072T= JCM 19985T).
authors.affiliation: Center for Bioanalysis, Korea Research Institute of Standards and Science, 267, Gajeong-ro, Yuseong-gu, Daejeon 305-340, Republic of Korea.
authors.forename: Seil
authors.initials: S
authors.lastname: Kim
authors.affiliation: Clean Energy Research Center, Korea Institute of Science and Technology, 5, Hwarang-ro 14-gil, Seongbuk-gu, Seoul 136-791, Republic of Korea.
authors.forename: Gyeongtaek
authors.initials: G
authors.lastname: Gong
authors.affiliation: Clean Energy Research Center, Korea Institute of Science and Technology, 5, Hwarang-ro 14-gil, Seongbuk-gu, Seoul 136-791, Republic of Korea.
authors.forename: Han
authors.initials: H
authors.lastname: Min Woo
authors.affiliation: Clean Energy Research Center, Korea Institute of Science and Technology, 5, Hwarang-ro 14-gil, Seongbuk-gu, Seoul 136-791, Republic of Korea.
authors.forename: Yunje
authors.initials: Y
authors.lastname: Kim
authors.affiliation: Clean Energy Research Center, Korea Institute of Science and Technology, 5, Hwarang-ro 14-gil, Seongbuk-gu, Seoul 136-791, Republic of Korea.
authors.forename: Youngsoon
authors.initials: Y
authors.lastname: Um