title: Induction of Human Regulatory T Cells with Bacterial Superantigens.
abstract: Regulatory T cells (Tregs) that suppress the activation of immune effector cells limit immunopathology and are fast emerging as therapeutic targets for autoimmune and cancer disease. Tools enabling Treg in vitro-induction, expansion, and characterization and manipulation will help future clinical developments. In this chapter, we describe in detail how to use bacterial superantigens to induce human Tregs efficiently from peripheral blood mononuclear cells. How to assess human Treg phenotype and suppressive capacity are also described. Technical details, variations, and alternative experimental conditions are provided.
authors.affiliation: Division of Clinical Medicine, BSMS-Brighton and Sussex Medical School, University of Sussex, Medical Research Building, Falmer, BN1 9PS, UK. s.caserta@bsms.ac.uk.
authors.forename: Stefano
authors.initials: S
authors.lastname: Caserta
authors.affiliation: Division of Clinical Medicine, BSMS-Brighton and Sussex Medical School, University of Sussex, Medical Research Building, Falmer, BN1 9PS, UK.
authors.forename: Amanda L
authors.initials: AL
authors.lastname: Taylor
authors.affiliation: Pharmacy and Biomolecular Sciences, University of Brighton, Moulsecoomb, Brighton, BN2 4GJ, UK.
authors.forename: Nadia
authors.initials: N
authors.lastname: Terrazzini
authors.affiliation: Division of Clinical Medicine, BSMS-Brighton and Sussex Medical School, University of Sussex, Medical Research Building, Falmer, BN1 9PS, UK.|Brighton and Sussex University Hospitals NHS Trust, Brighton, BN2 5BE, UK.
authors.forename: Martin J
authors.initials: MJ
authors.lastname: Llewelyn
mesh_terms: D000954:Antigens, Surface / Q000378:metabolism; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D018920:Coculture Techniques; D005434:Flow Cytometry; D006801:Humans; D018189:Immunomagnetic Separation / Q000379:methods; D056747:Immunomodulation; D016130:Immunophenotyping; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000378:metabolism; D008213:Lymphocyte Activation / Q000276:immunology*; D010641:Phenotype; D054504:Precursor Cells, T-Lymphoid / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D018089:Superantigens / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology* / Q000378:metabolism*
chemical_list: D000954:Antigens, Surface; D018089:Superantigens
keywords: Cell purification; Human; Immunological methods; PBMC; Regulatory T cells; Superantigen; Superantigen stimulation; Suppression assay; Treg phenotype