title: Macrophage phenotypes in the adipose tissue of postmenopausal women.
abstract: Atherosclerosis pathology is the interplay between high intravascular LDL particle concentration and monocyte/macrophage presence within the sub-endothelial space of the artery. In this project, phenotypes of macrophages connected with subclinical inflammation in adipose tissue of living kidney donors were studied. Samples of subcutaneous adipose tissue of living kidney donors (n=36) were exposed to collagenase. Stromal vascular fraction (SVF) was eluted from the samples, then labeled with monoclonal antibodies (anti-CD14 and anti-calprotectin), conjugated with fluorochromes and analyzed by flow cytometry. The positive correlation between the number of total macrophages and calprotectin-positive macrophages with BMI in the subcutaneous adipose tissue of postmenopausal women was demonstrated (p<0.05; R=0.43 and p<0.01; R=0.60), whereas no positive correlation in premenopausal women and men was shown. In conclusion, we documented a significant effect of BMI increase on the presence of total macrophages in adipose tissue of postmenopausal women, in contrast to premenopausal women. This difference was much more pronounced when proinflammatory macrophages with membrane-bound calprotectin were analyzed.
authors.affiliation: Laboratory for Atherosclerosis Research, Centre for Experimental Medicine, Institute for Clinical and Experimental Medicine (IKEM), Prague, Czech Republic. rupo@ikem.cz.
authors.forename: A
authors.initials: A
authors.lastname: Králová
authors.forename: I
authors.initials: I
authors.lastname: Králová Lesná
authors.forename: J
authors.initials: J
authors.lastname: Froněk
authors.forename: S
authors.initials: S
authors.lastname: Čejková
authors.forename: A
authors.initials: A
authors.lastname: Sekerková
authors.forename: L
authors.initials: L
authors.lastname: Janoušek
authors.forename: F
authors.initials: F
authors.lastname: Thieme
authors.forename: I
authors.initials: I
authors.lastname: StříŽ
authors.forename: J
authors.initials: J
authors.lastname: Ždychová
authors.forename: R
authors.initials: R
authors.lastname: Poledne
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism; D000328:Adult; D015992:Body Mass Index; D005260:Female; D006801:Humans; D008264:Macrophages / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D017698:Postmenopause / Q000378:metabolism*; D050151:Subcutaneous Fat / Q000378:metabolism*