title: Influence of Environmental Factors on Bacteriocin Production by Human Isolates of Lactococcus lactis MM19 and Pediococcus acidilactici MM33.
abstract: The influence of temperature, initial pH, and carbon and nitrogen sources on bacteriocin secreted by Lactococcus lactis MM19 (MM19) and Pediococcus acidilactici MM33 (MM33) was evaluated. It was found that 30 and 45 °C were the growth temperatures for higher nisin and pediocin production by MM19 and MM33, respectively. The initial pH values for higher production of nisin and pediocin were 9 and 6, respectively. Glucose and wheat peptone E430 were found as suitable carbon and nitrogen sources, respectively, for highest nisin production by MM19 at 30 °C and initial pH of 9. In these conditions, nisin production could be increased by 6.7 times as compared to the control medium (de Man, Rogosa, and Sharpe--MRS broth). Similarly, fructose and pea peptone were suitable carbon and nitrogen sources, respectively, for highest production of pediocin by MM33 at 45 °C and initial pH of 6. In these conditions, pediocin production by MM33 was increased by three times as compared to the control medium (tryptone-glucose-yeast extract-TGE broth).
authors.affiliation: Research Laboratories in Sciences Applied to Food, Canadian Irradiation Center, Institute of Nutraceuticals and Functional Foods (INAF), INRS-Institut Armand-Frappier, 531, Boulevard des Prairies, Laval, QC, H7V 1B7, Canada.
authors.forename: Mélanie
authors.initials: M
authors.lastname: Turgis
authors.affiliation: Research Laboratories in Sciences Applied to Food, Canadian Irradiation Center, Institute of Nutraceuticals and Functional Foods (INAF), INRS-Institut Armand-Frappier, 531, Boulevard des Prairies, Laval, QC, H7V 1B7, Canada.
authors.forename: Khanh Dang
authors.initials: KD
authors.lastname: Vu
authors.affiliation: BioK Plus International Inc., 495 Boulevard Armand Frappier, Laval, QC, H7V 4B3, Canada.
authors.forename: Mathieu
authors.initials: M
authors.lastname: Millette
authors.affiliation: BioK Plus International Inc., 495 Boulevard Armand Frappier, Laval, QC, H7V 4B3, Canada.
authors.forename: Claude
authors.initials: C
authors.lastname: Dupont
authors.affiliation: Research Laboratories in Sciences Applied to Food, Canadian Irradiation Center, Institute of Nutraceuticals and Functional Foods (INAF), INRS-Institut Armand-Frappier, 531, Boulevard des Prairies, Laval, QC, H7V 1B7, Canada. Monique.Lacroix@iaf.inrs.ca.
authors.forename: Monique
authors.initials: M
authors.lastname: Lacroix
mesh_terms: D001430:Bacteriocins / Q000096:biosynthesis*; D002244:Carbon / Q000378:metabolism; D003470:Culture Media; D005243:Feces / Q000382:microbiology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D013294:Lactococcus lactis / Q000302:isolation & purification / Q000378:metabolism*; D009584:Nitrogen / Q000378:metabolism; D010376:Pediococcus / Q000302:isolation & purification / Q000378:metabolism*; D013696:Temperature
chemical_list: D001430:Bacteriocins; D003470:Culture Media; D002244:Carbon; D009584:Nitrogen
keywords: Culture media; Lactococcus lactis; Nisin; Pediocin; Pediococcus acidilactici