title: Toxicity and bioaccumulation of copper in Limnodrilus hoffmeisteri under different pH values: Impacts of perfluorooctane sulfonate.
abstract: Aquatic oligochaete Limnodrilus hoffmeisteri (L. hoffmeisteri) has been commonly used as a lethal and/or sub-lethal toxicological model organism in ecological risk assessments in contaminated water environments. In this study, experiments were conducted to investigate the potential toxic effects of copper (Cu(II)) with or without perfluorooctane sulfonate (PFOS) under different pH values (6.0, 7.0 and 8.0) on LC50, bioaccumulation, and oxidative stress biomarkers in L. hoffmeisteri after 3 and 7 days. The LC50 values of Cu(II) decreased with the increasing pH and the addition of PFOS. After each exposure, increasing bioaccumulation of Cu(II) in L. hoffmeisteri was observed in the combined exposure treatments, whereas the bioaccumulation of PFOS decreased. Moreover, the activity of superoxide dismutase, the level of glutathione, and the content of malondialdehyde were significantly altered after these exposures, possibly indicating that the bioaccumulation of Cu(II) and PFOS caused adverse effects on antioxidant defenses of L. hoffmeisteri. The integrated biomarker response index, indicates that the combined effect was proposed as synergism, which is coincided with the results of toxic unit. Moreover, this work showed that aquatic environment may become more livable when water conditions changed from acidic to near-neutral or alkaline.
authors.affiliation: State Key Laboratory of Pollution Control and Resources Reuse, School of the Environment, Nanjing University, Nanjing, Jiangsu 210023, PR China.
authors.forename: Lingjun
authors.initials: L
authors.lastname: Meng
authors.affiliation: State Key Laboratory of Pollution Control and Resources Reuse, School of the Environment, Nanjing University, Nanjing, Jiangsu 210023, PR China. Electronic address: yangsg@nju.edu.cn.
authors.forename: Shaogui
authors.initials: S
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Pollution Control and Resources Reuse, School of the Environment, Nanjing University, Nanjing, Jiangsu 210023, PR China.
authors.forename: Mingbao
authors.initials: M
authors.lastname: Feng
authors.affiliation: State Key Laboratory of Pollution Control and Resources Reuse, School of the Environment, Nanjing University, Nanjing, Jiangsu 210023, PR China.
authors.forename: Ruijuan
authors.initials: R
authors.lastname: Qu
authors.affiliation: State Key Laboratory of Pollution Control and Resources Reuse, School of the Environment, Nanjing University, Nanjing, Jiangsu 210023, PR China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Li
authors.affiliation: State Key Laboratory of Pollution Control and Resources Reuse, School of the Environment, Nanjing University, Nanjing, Jiangsu 210023, PR China.
authors.forename: Jiaoqin
authors.initials: J
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Pollution Control and Resources Reuse, School of the Environment, Nanjing University, Nanjing, Jiangsu 210023, PR China. Electronic address: wangzy@nju.edu.cn.
authors.forename: Zunyao
authors.initials: Z
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Pollution Control and Resources Reuse, School of the Environment, Nanjing University, Nanjing, Jiangsu 210023, PR China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Sun
mesh_terms: D017738:Alkanesulfonic Acids / Q000494:pharmacology / Q000633:toxicity*; D000818:Animals; D003300:Copper / Q000493:pharmacokinetics / Q000633:toxicity*; D005466:Fluorocarbons / Q000494:pharmacology / Q000633:toxicity*; D005978:Glutathione / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007928:Lethal Dose 50; D008315:Malondialdehyde / Q000378:metabolism; D009835:Oligochaeta / Q000187:drug effects* / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000493:pharmacokinetics / Q000633:toxicity*
chemical_list: D017738:Alkanesulfonic Acids; D005466:Fluorocarbons; D017382:Reactive Oxygen Species; D014874:Water Pollutants, Chemical; D008315:Malondialdehyde; D003300:Copper; C076994:perfluorooctane sulfonic acid; D013482:Superoxide Dismutase; D005978:Glutathione
keywords: Bioaccumulation; Copper; Different pH values; Limnodrilus hoffmeisteri; Oxidative stress; Perfluorooctane sulfonate