title: Design and synthesis of magnetic binary metal oxides nanocomposites through dopamine chemistry for highly selective enrichment of phosphopeptides.
abstract: In this work, for the first time, magnetic binary metal oxides nanocomposites which integrated Zr and Ti into one entity on an atomic scale on polydopamine coated magnetic graphene (magG/PD/(Zr-Ti)O4 ) was designed and synthesized, and applied to the enrichment of phosphopeptides. The newly prepared magG/PD/(Zr-Ti)O4 composites gathered the advantages of large surface area, superparamagnetism, biocompatibility and the enhanced affinity properties to phosphopeptides. MagG/PD/ZrO2 , magG/PD/TiO2 , as well as the simple physical mixture of them were introduced to compare with magG/PD/(Zr-Ti)O4 composites. High sensitivity (1 pg/μL or 4.0 × 10(-11) M) and selectivity (weight ratio of β-casein and BSA reached up to 1:8000) toward phosphopeptides were also presented for magG/PD/(Zr-Ti)O4 composites. Additionally, mouse brain tissue was chose as the real samples to further investigate the phosphopeptides enrichment ability of this new material.
authors.affiliation: Department of Chemistry Fudan University, Shanghai, P. R. China.|Institute of Biomedical Sciences, Fudan University, Shanghai, P. R. China.
authors.forename: Mengyi
authors.initials: M
authors.lastname: Wang
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Fudan University, Shanghai, P. R. China.
authors.forename: Xueni
authors.initials: X
authors.lastname: Sun
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Fudan University, Shanghai, P. R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Chemistry Fudan University, Shanghai, P. R. China.|Institute of Biomedical Sciences, Fudan University, Shanghai, P. R. China.
authors.forename: Chunhui
authors.initials: C
authors.lastname: Deng
mesh_terms: D000818:Animals; D001923:Brain Chemistry; D002846:Chromatography, Affinity; D006108:Graphite / Q000737:chemistry; D007211:Indoles / Q000737:chemistry*; D059346:Magnets / Q000737:chemistry*; D019216:Metals, Heavy / Q000737:chemistry*; D051379:Mice; D053761:Nanocomposites / Q000737:chemistry*; D010087:Oxides / Q000737:chemistry*; D010748:Phosphopeptides / Q000032:analysis / Q000302:isolation & purification*; D011108:Polymers / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D007211:Indoles; D019216:Metals, Heavy; D010087:Oxides; D010748:Phosphopeptides; D011108:Polymers; C568283:polydopamine; D006108:Graphite
keywords: Binary metal oxide affinity chromatography; Enrichment; MALDI TOF MASS; Phosphopeptides; Technology; magG/PD/(Zr-Ti)O4