title: The complete chloroplast genome sequence of Cucumis sativus var. Hardwickii, the wild progenitor of cultivated cucumber.
abstract: The complete chloroplast genome sequence of wild cucumber (Cucumis sativus var. hardwickii) was determined and characterized in this study. The genome is of 155 277 bp in length, containing a pair of inverted repeats regions (IRs) of 25 198 bp, which are separated by a large single-copy region of 86 618 bp and a small single-copy region of 18 263 bp. The wild cucumber chloroplast genome has 130 known genes, including 85 protein-coding genes, 8 ribosomal RNA genes, and 37 tRNA genes. Among these genes, 19 comprise one or two introns. There are 11 tRNA genes present in the IR of the chloroplast genome. Phylogenomic analysis showed that C. sativus var. hardwickii forms a cluster with other Cucumis species with strong bootstrap supports and is closely related to C. sativus var. sativus. This newly sequenced chloroplast genome sequence may provide useful genetic information to explore wild cucumber germplasms for cucumber breeding programs.
authors.affiliation: a Faculty of Environmental Science and Engineering and.|b Faculty of Life Science and Technology , Kunming University of Science and Technology , Kunming , China.
authors.forename: Bang
authors.initials: B
authors.lastname: Liu
authors.affiliation: a Faculty of Environmental Science and Engineering and.|b Faculty of Life Science and Technology , Kunming University of Science and Technology , Kunming , China.
authors.forename: Dan
authors.initials: D
authors.lastname: Zhang
authors.affiliation: a Faculty of Environmental Science and Engineering and.|b Faculty of Life Science and Technology , Kunming University of Science and Technology , Kunming , China.
authors.forename: Li-Zhi
authors.initials: LZ
authors.lastname: Gao
mesh_terms: D002736:Chloroplasts / Q000235:genetics*; D018553:Cucumis sativus / Q000145:classification / Q000235:genetics*; D019662:Cucurbitaceae / Q000145:classification / Q000235:genetics; D018742:DNA, Chloroplast / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D054628:Genome, Chloroplast; D007438:Introns; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012335:RNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D012343:RNA, Transfer / Q000737:chemistry / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D018742:DNA, Chloroplast; D010940:Plant Proteins; D012335:RNA, Ribosomal; D012343:RNA, Transfer
keywords: Cucumis sativus var. hardwickii; chloroplast genome; wild cucumber