title: Upregulation of EMMPRIN (OX47) in Rat Dorsal Root Ganglion Contributes to the Development of Mechanical Allodynia after Nerve Injury.
abstract: Matrix metalloproteinases (MMPs) are widely implicated in inflammation and tissue remodeling associated with various neurodegenerative diseases and play an important role in nociception and allodynia. Extracellular Matrix Metalloproteinase Inducer (EMMPRIN) plays a key regulatory role for MMP activities. However, the role of EMMPRIN in the development of neuropathic pain is not clear. Western blotting, real-time quantitative RT-PCR (qRT-PCR), and immunofluorescence were performed to determine the changes of messenger RNA and protein of EMMPRIN/OX47 and their cellular localization in the rat dorsal root ganglion (DRG) after nerve injury. Paw withdrawal threshold test was examined to evaluate the pain behavior in spinal nerve ligation (SNL) model. The lentivirus containing OX47 shRNA was injected into the DRG one day before SNL. The expression level of both mRNA and protein of OX47 was markedly upregulated in ipsilateral DRG after SNL. OX47 was mainly expressed in the extracellular matrix of DRG. Administration of shRNA targeted against OX47 in vivo remarkably attenuated mechanical allodynia induced by SNL. In conclusion, peripheral nerve injury induced upregulation of OX47 in the extracellular matrix of DRG. RNA interference against OX47 significantly suppressed the expression of OX47 mRNA and the development of mechanical allodynia. The altered expression of OX47 may contribute to the development of neuropathic pain after nerve injury.
authors.affiliation: Department of Anesthesiology and Pain Management, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Qun
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Department of Anesthesiology and Pain Management, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Yanyuan
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Department of Anesthesiology and Pain Management, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Yingna
authors.initials: Y
authors.lastname: Ren
authors.affiliation: Department of Anesthesiology, Yulin First Hospital, Yulin 710021, China.
authors.forename: Yandong
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Department of Anesthesiology and Pain Management, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Li
authors.initials: L
authors.lastname: Tian
authors.affiliation: Department of Anesthesiology and Pain Management, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Anesthesiology and Pain Management, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Yanan
authors.initials: Y
authors.lastname: Pu
authors.affiliation: Institute of Basic Medical Science, Xi'an Medical University, Xi'an 710021, China.
authors.forename: Xingchun
authors.initials: X
authors.lastname: Gou
authors.affiliation: Experiment Center of Biomedical Research, School of Medicine, Xi'an Jiaotong University, Xi'an 710061, China.
authors.forename: Yanke
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Anesthesiology and Pain Management, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Lu
mesh_terms: D000818:Animals; D051926:Basigin / Q000378:metabolism*; D005109:Extracellular Matrix / Q000378:metabolism; D005727:Ganglia, Spinal / Q000378:metabolism*; D006930:Hyperalgesia / Q000378:metabolism*; D008297:Male; D017288:Pain Threshold / Q000502:physiology; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013127:Spinal Nerves / Q000293:injuries*; D015854:Up-Regulation
chemical_list: D012333:RNA, Messenger; D051926:Basigin