title: An epigenetic biomarker combination of PCDH17 and POU4F2 detects bladder cancer accurately by methylation analyses of urine sediment DNA in Han Chinese.
abstract: To develop a routine and effectual procedure of detecting bladder cancer (BlCa), an optimized combination of epigenetic biomarkers that work synergistically with high sensitivity and specificity is necessary. In this study, methylation levels of seven biomarkers (EOMES, GDF15, NID2, PCDH17, POU4F2, TCF21, and ZNF154) in 148 individuals-which including 58 urothelial cell carcinoma (UCC) patients, 20 infected urinary calculi (IUC) patients, 20 kidney cancer (KC) patients,20 prostate cancer (PC) patients, and 30 healthy volunteers (HV)-were quantified by qMSP using the urine sediment DNA. Receiver operating characteristic (ROC) curves were generated for each biomarker. The combining predictors of possible combinations were calculated through logistic regression model. Subsequently, ROC curves of the three best performing combinations were constructed. Then, we validated the three best performing combinations and POU4F2 in another 72 UCC, 21 IUC, 26 KC and 22 PC, and 23 HV urine samples. The combination of POU4F2/PCDH17 has yielded the highest sensitivity and specificity of 90.00% and 93.96% in all the 312 individuals, showing the capability of detecting BlCa effectively among pathologically varied sample groups.
authors.affiliation: Department of Urology, Sun Yat-sen University Cancer Center, Collaborative Innovation Center for Cancer Medicine, State Key Laboratory of Oncology in South China, Guangzhou, 510060, China.|The Affiliated Luohu Hospital of Shenzhen University, Shenzhen Luohu Hospital Group, Shenzhen 518000, China.
authors.forename: Yongqiang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: BGI-Shenzhen, Shenzhen 518083, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yu
authors.affiliation: BGI-Shenzhen, Shenzhen 518083, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Ye
authors.affiliation: BGI-Shenzhen, Shenzhen 518083, China.
authors.forename: Duo
authors.initials: D
authors.lastname: Zhang
authors.affiliation: BGI-Shenzhen, Shenzhen 518083, China.
authors.forename: Qiaoling
authors.initials: Q
authors.lastname: Li
authors.affiliation: BGI-Shenzhen, Shenzhen 518083, China.
authors.forename: Dan
authors.initials: D
authors.lastname: An
authors.affiliation: Anhui Medical University, Hefei 230032, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Fang
authors.affiliation: Department of Urology, Zhujiang Hospital of Southern Medical University, Guangzhou 510280, China.
authors.forename: Youcheng
authors.initials: Y
authors.lastname: Lin
authors.affiliation: BGI-Shenzhen, Shenzhen 518083, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Hou
authors.affiliation: Department of Urology, Zhujiang Hospital of Southern Medical University, Guangzhou 510280, China.
authors.forename: Abai
authors.initials: A
authors.lastname: Xu
authors.affiliation: The Second People's Hospital of Guangdong Province, Guangzhou 510310, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Fu
authors.affiliation: Anhui Medical University, Hefei 230032, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Lu
authors.affiliation: Department of Urology, Sun Yat-sen University Cancer Center, Collaborative Innovation Center for Cancer Medicine, State Key Laboratory of Oncology in South China, Guangzhou, 510060, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Chen
authors.affiliation: Anhui Medical University, Hefei 230032, China.
authors.forename: Mingwei
authors.initials: M
authors.lastname: Chen
authors.affiliation: Anhui Medical University, Hefei 230032, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Zhang
authors.affiliation: The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510080, China.
authors.forename: Huiling
authors.initials: H
authors.lastname: Jiang
authors.affiliation: Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou 510080, China.
authors.forename: Chuanxia
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Urology, Sun Yat-sen University Cancer Center, Collaborative Innovation Center for Cancer Medicine, State Key Laboratory of Oncology in South China, Guangzhou, 510060, China.
authors.forename: Pei
authors.initials: P
authors.lastname: Dong
authors.affiliation: CAS Key Laboratory of Infection and Immunity, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Chong
authors.initials: C
authors.lastname: Li
authors.affiliation: The Third Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510630, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Chen
authors.affiliation: The Second People's Hospital of Guangdong Province, Guangzhou 510310, China.
authors.forename: Guosheng
authors.initials: G
authors.lastname: Yang
authors.affiliation: Department of Urology, Zhujiang Hospital of Southern Medical University, Guangzhou 510280, China.
authors.forename: Chunxiao
authors.initials: C
authors.lastname: Liu
authors.affiliation: Department of Urological Surgery, Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen 518000, China.
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Cai
authors.affiliation: Department of Urology, Sun Yat-sen University Cancer Center, Collaborative Innovation Center for Cancer Medicine, State Key Laboratory of Oncology in South China, Guangzhou, 510060, China.
authors.forename: Fangjian
authors.initials: F
authors.lastname: Zhou
authors.affiliation: The Affiliated Luohu Hospital of Shenzhen University, Shenzhen Luohu Hospital Group, Shenzhen 518000, China.|Department of Urological Surgery, Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen 518000, China.
authors.forename: Song
authors.initials: S
authors.lastname: Wu
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics / Q000652:urine*; D015820:Cadherins / Q000235:genetics*; D002681:China; D004247:DNA / Q000235:genetics / Q000652:urine; D019175:DNA Methylation / Q000235:genetics*; D006801:Humans; D007680:Kidney Neoplasms / Q000652:urine; D008297:Male; D011471:Prostatic Neoplasms / Q000652:urine; D012680:Sensitivity and Specificity; D050820:Transcription Factor Brn-3B / Q000235:genetics*; D001749:Urinary Bladder Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000652:urine*; D014545:Urinary Calculi / Q000652:urine; D019459:Urothelium / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; D015820:Cadherins; C529349:PCDH17 protein, human; C494190:POU4F2 protein, human; D050820:Transcription Factor Brn-3B; D004247:DNA
keywords: biomarkers; bladder cancer; epigenetics; methylation; qMSP