title: Evaluation of chromogenic agar, [corrected] VITEK2 YST and VITEK® MS for identification of Candida strains isolated from blood cultures.
abstract: The aim of this study is to compare conventional methods, chromogenic agar, [corrected] VITEK2 YST card and VITEK®MS system for the identification of Candida strains isolated from blood cultures. Fifty-four strains were identified according to conventional methods, chromogenic agar, [corrected] VITEK2 YST card and VITEK®MS. Sequencing was used as the reference method. The 54 strains included 32 Candida parapsilosis, 19 Candida albicans, 1 Candida glabrata and 2 Candida tropicalis according to the reference method. One C. albicans and one C. glabrata isolate were misidentified as C. parapsilosis by chromogenic agar. [corrected]. Two C. parapsilosis and three C. albicans isolates were misidentified by VITEK2 YST card. Chromogenic agar, [corrected] VITEK2 YST card and VITEK®MS identified correctly 96.2%, 90.7% and 100% of all strains, respectively. We found that the chromogenic agar, [corrected] VITEK2 YST card and VITEK®MS system are easy, rapid and accurate alternative methods for the identification of yeast species in the clinical microbiology laboratory.
authors.affiliation: Department of Microbiology, Kayseri Education and Research Hospital, Kayseri, Turkey.
authors.forename: Fatma Mutlu
authors.initials: FM
authors.lastname: Sariguzel
authors.affiliation: Department of Microbiology, Erciyes University Medical School, Kayseri, Turkey.
authors.forename: Elife
authors.initials: E
authors.lastname: Berk
authors.affiliation: Department of Microbiology, Erciyes University Medical School, Kayseri, Turkey.
authors.forename: Ayse Nedret
authors.initials: AN
authors.lastname: Koc
authors.affiliation: Department of Microbiology, Erciyes University Medical School, Kayseri, Turkey.
authors.forename: Hafize
authors.initials: H
authors.lastname: Sav
authors.affiliation: Department of Microbiology, Erciyes University Medical School, Kayseri, Turkey.
authors.forename: Gonca
authors.initials: G
authors.lastname: Aydemir
mesh_terms: D000362:Agar; D000071997:Blood Culture / Q000379:methods; D002175:Candida / Q000145:classification* / Q000302:isolation & purification*; D002176:Candida albicans / Q000302:isolation & purification; D041221:Candida glabrata / Q000302:isolation & purification; D041022:Candida tropicalis / Q000302:isolation & purification; D002177:Candidiasis / Q000175:diagnosis* / Q000382:microbiology*; D003470:Culture Media; D006801:Humans; D016533:Mycological Typing Techniques / Q000379:methods*; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity
chemical_list: D003470:Culture Media; D000362:Agar