title: Molecular Characterization of Five Potyviruses Infecting Korean Sweet Potatoes Based on Analyses of Complete Genome Sequences.
abstract: Sweet potatoes (Ipomea batatas L.) are grown extensively, in tropical and temperate regions, and are important food crops worldwide. In Korea, potyviruses, including Sweet potato feathery mottle virus (SPFMV), Sweet potato virus C (SPVC), Sweet potato virus G (SPVG), Sweet potato virus 2 (SPV2), and Sweet potato latent virus (SPLV), have been detected in sweet potato fields at a high (~95%) incidence. In the present work, complete genome sequences of 18 isolates, representing the five potyviruses mentioned above, were compared with previously reported genome sequences. The complete genomes consisted of 10,081 to 10,830 nucleotides, excluding the poly-A tails. Their genomic organizations were typical of the Potyvirus genus, including one target open reading frame coding for a putative polyprotein. Based on phylogenetic analyses and sequence comparisons, the Korean SPFMV isolates belonged to the strains RC and O with >98% nucleotide sequence identity. Korean SPVC isolates had 99% identity to the Japanese isolate SPVC-Bungo and 70% identity to the SPFMV isolates. The Korean SPVG isolates showed 99% identity to the three previously reported SPVG isolates. Korean SPV2 isolates had 97% identity to the SPV2 GWB-2 isolate from the USA. Korean SPLV isolates had a relatively low (88%) nucleotide sequence identity with the Taiwanese SPLV-TW isolates, and they were phylogenetically distantly related to SPFMV isolates. Recombination analysis revealed that possible recombination events occurred in the P1, HC-Pro and NIa-NIb regions of SPFMV and SPLV isolates and these regions were identified as hotspots for recombination in the sweet potato potyviruses.
authors.affiliation: Crop Protection Division, National Academy of Agricultural Science, Wanju 565-851, Korea.
authors.forename: Hae-Ryun
authors.initials: HR
authors.lastname: Kwak
authors.affiliation: Crop Protection Division, National Academy of Agricultural Science, Wanju 565-851, Korea ; Department of Genetic Engineering, Sungkyunkwan University, Suwon 440-746, Korea.
authors.forename: Jaedeok
authors.initials: J
authors.lastname: Kim
authors.affiliation: Crop Protection Division, National Academy of Agricultural Science, Wanju 565-851, Korea.
authors.forename: Mi-Kyeong
authors.initials: MK
authors.lastname: Kim
authors.affiliation: Crop Protection Division, National Academy of Agricultural Science, Wanju 565-851, Korea.
authors.forename: Jang-Kyun
authors.initials: JK
authors.lastname: Seo
authors.affiliation: Bioenergy Crop Research Center, National Institute of Crop Science, Muan 534-833, Korea.
authors.forename: Mi-Nam
authors.initials: MN
authors.lastname: Jung
authors.affiliation: Department of Plant Medicine, Andong National University, Andong 760-749, Korea.
authors.forename: Jeong-Soo
authors.initials: JS
authors.lastname: Kim
authors.affiliation: Department of Genetic Engineering, Sungkyunkwan University, Suwon 440-746, Korea.
authors.forename: Sukchan
authors.initials: S
authors.lastname: Lee
authors.affiliation: Crop Protection Division, National Academy of Agricultural Science, Wanju 565-851, Korea.
authors.forename: Hong-Soo
authors.initials: HS
authors.lastname: Choi
keywords: complete genomes; phylogenetic analyses; recombination; sweet potato viruses