title: Development of EST-SSR markers in Barringtonia racemosa (Lecythidaceae) and cross-amplification in related species.
abstract: OBJECTIVE
  Microsatellite markers were identified and characterized to study the genetic diversity and structure of Barringtonia racemosa (Lecythidaceae).
  
  
  RESULTS
  Based on the transcriptome data of B. racemosa, 30 primer pairs were initially designed and tested, of which 15 were successfully amplified and displayed clear polymorphisms across the 43 individuals from three distant populations tested in the study. The results showed that the number of alleles per locus ranged from two to seven and the expected heterozygosity and observed heterozygosity per locus varied from 0 to 0.772 and from 0 to 0.933, respectively.
  
  
  CONCLUSIONS
  The expressed sequence tag-simple sequence repeat (EST-SSR) markers described here will be useful for studying genetic diversity and structure of B. racemosa. Furthermore, all loci were successfully cross-amplified in B. asiatica and B. acutangula and will be of great value for genetic studies across this genus.
authors.affiliation: Guangdong Key Laboratory of Plant Resources and State Key Laboratory of Biocontrol, Sun Yat-sen University, Guangzhou 510275, Guangdong, People's Republic of China.
authors.forename: Hongxian
authors.initials: H
authors.lastname: Xie
authors.affiliation: Guangdong Key Laboratory of Plant Resources and State Key Laboratory of Biocontrol, Sun Yat-sen University, Guangzhou 510275, Guangdong, People's Republic of China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: Guangdong Key Laboratory of Plant Resources and State Key Laboratory of Biocontrol, Sun Yat-sen University, Guangzhou 510275, Guangdong, People's Republic of China ; Division of Life Science, The Hong Kong University of Science and Technology, Hong Kong, People's Republic of China.
authors.forename: Xiaoting
authors.initials: X
authors.lastname: Fang
authors.affiliation: Guangdong Key Laboratory of Plant Resources and State Key Laboratory of Biocontrol, Sun Yat-sen University, Guangzhou 510275, Guangdong, People's Republic of China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, Guangdong, People's Republic of China.
authors.forename: Chao
authors.initials: C
authors.lastname: Yang
authors.affiliation: Guangdong Key Laboratory of Plant Resources and State Key Laboratory of Biocontrol, Sun Yat-sen University, Guangzhou 510275, Guangdong, People's Republic of China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Jin
authors.affiliation: College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, Guangdong, People's Republic of China.
authors.forename: Fengxiao
authors.initials: F
authors.lastname: Tan
authors.affiliation: Guangdong Key Laboratory of Plant Resources and State Key Laboratory of Biocontrol, Sun Yat-sen University, Guangzhou 510275, Guangdong, People's Republic of China.
authors.forename: Yelin
authors.initials: Y
authors.lastname: Huang
keywords: Barringtonia racemosa; EST-SSR; Lecythidaceae; cross-amplification; transcriptome