title: Gene Expression Profiling of Non-Small Cell Lung Cancer.
abstract: OBJECTIVE
  cDNA microarray provided a powerful alternative, with an unprecedented view scope, in monitoring gene expression levels, and led to the discovery of regulatory pathways involved in complicated biological processes. This study was performed to gain better understanding of the molecular mechanisms underlying the carcinogenesis and progression of lung cancer.
  
  
  METHODS
  Using a cDNA microarray, representing 4, 600 cDNA clusters, we studied the expression profiles in 10 non-small cell lung cancer (NSCLC) samples and the adjacent noncancerous lung tissues form the same patients. The alterations in the levels of gene expression were confirmed by reverse-transcription PCR in 10 randomly selected genes.
  
  
  RESULTS
  Genes that were differently expressed in the cancerous and noncancerous tissues were identified. One hundred and nine genes (of which 68 were known) and 69 cDNAs (of which 32 were known) were up- and down-regulated in>70% of the NSCLC samples, respectively. In the cancerous tissues, the genes related to the cell cycle, metabolism, cell structure and signal transduction, were mostly up-regulated. Furthermore, we identified a few putative tumor suppressor genes that had previously been proposed by other workers.
  
  
  CONCLUSIONS
  S: These results provide, not only a new molecular basis for understanding the biological properties of NSCLC, but also useful resources for the future development of diagnostic markers and therapeutic targets for NSCLC.
authors.forename: Mee Sook
authors.initials: MS
authors.lastname: Roh
authors.forename: Hyuk Chan
authors.initials: HC
authors.lastname: Kwon
authors.forename: Jin Sook
authors.initials: JS
authors.lastname: Jeong
authors.forename: Dae Cheol
authors.initials: DC
authors.lastname: Kim
authors.forename: Choon Hee
authors.initials: CH
authors.lastname: Son
authors.forename: Soo Keol
authors.initials: SK
authors.lastname: Lee
authors.forename: Phil Jo
authors.initials: PJ
authors.lastname: Choi
authors.forename: Jae Ik
authors.initials: JI
authors.lastname: Lee
authors.forename: Ki Nam
authors.initials: KN
authors.lastname: Lee
authors.forename: Hyo Jin
authors.initials: HJ
authors.lastname: Kim
authors.forename: Jin Han
authors.initials: JH
authors.lastname: Yoon
authors.forename: Tae Ho
authors.initials: TH
authors.lastname: Hwang
keywords: Gene expression; Non- small cell lung cancer; cDNA microarray