title: [Role of long non-coding RNA BC088414 in hypoxic-ischemic injury of neural cells].
abstract: OBJECTIVE
  To investigate the role of long non-coding RNA (lncRNA) BC088414 in hypoxic-ischemic injury of neural cells.
  
  
  METHODS
  Rat adrenal pheochromocytoma (PC12) cells were divided into four groups: normoxic, oxygen glucose deprivation (OGD), siRNA-normoxic (siRNA group) and siRNA-OGD (n=3 each). Cells were incubated in glucose-free and serum-free DMEM medium under the conditions of 37℃ and 1% O2+99% N2/CO2 for 6 hours to establish an in vitro hypoxic-ischemic model. Quantitative real-time PCR was used to measure mRNA expression of lncRNA BC088414, β2-adrenoceptor (Adrb2), and caspase-6 (CASP6). siRNAs were used to inhibit BC088414 expression in PC12 cells. The TUNEL method was used to measure cell apoptosis.
  
  
  RESULTS
  The OGD group had a significantly higher cell apoptotic index than the normoxic group (P<0.01). After inhibition of BC088414 expression, the OGD group had a significantly reduced apoptotic index (P<0.05). The OGD group had significantly higher mRNA expression levels of lncRNA BC088414, Adrb2, and CASP6 compared with the normoxic group (P<0.05). The siRNA -normoxic group had significantly lower mRNA expression levels of Adrb2 and CASP6 than the normoxic group (P<0.05), and the siRNA-OGD group also had significantly lower mRNA expression levels of Adrb2 and CASP6 than the OGD group (P<0.05).
  
  
  CONCLUSIONS
  LncRNA BC088414 may promote apoptosis through Adrb2 and CASP6 and aggravate neural cell injury induced by hypoxia-ischemia.
authors.affiliation: Departement of Pediatrics, West China Second Hospital, Sichuan University, Chengdu 610041, China. tj1234753@sina.com.
authors.forename: Feng-Yan
authors.initials: FY
authors.lastname: Zhao
authors.forename: Jun
authors.initials: J
authors.lastname: Tang
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Shi-Ping
authors.initials: SP
authors.lastname: Li
authors.forename: Yi
authors.initials: Y
authors.lastname: Feng
authors.forename: Hai-Ting
authors.initials: HT
authors.lastname: Liu
authors.forename: Yi
authors.initials: Y
authors.lastname: Qu
authors.forename: De-Zhi
authors.initials: DZ
authors.lastname: Mu
mesh_terms: D000818:Animals; D017209:Apoptosis; D053178:Caspase 6 / Q000235:genetics / Q000502:physiology; D015687:Cell Hypoxia; D009474:Neurons / Q000473:pathology*; D016716:PC12 Cells; D062085:RNA, Long Noncoding / Q000502:physiology*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D018343:Receptors, Adrenergic, beta-2 / Q000235:genetics / Q000502:physiology
chemical_list: D062085:RNA, Long Noncoding; D012333:RNA, Messenger; D018343:Receptors, Adrenergic, beta-2; D053178:Caspase 6