title: MiR-28-3p as a potential plasma marker in diagnosis of pulmonary embolism.
abstract: OBJECTIVE
  Circulating miRNAs have been reported to have potential in detecting various diseases. However, few studies explored differentially expressed miRNAs in plasma of patients with pulmonary embolism (PE). Our study is to identify plasma miRNAs which can serve as potential biomarkers of PE.
  
  
  METHODS
  Exiqon miRCURY Ready-to-Use PCR Human panel I+II V1.M was conducted to identify differently expressed miRNAs in pooled plasma samples of PE patients compared with normal controls. Expressions of identified miRNAs were assessed in 37 PE patients as well as matched normal individuals followed by validation on six Beagle dogs by quantitative reverse transcription polymerase chain reaction (qRT-PCR).
  
  
  RESULTS
  Twelve miRNAs were identified from the screening phase. Moreover, miR-134, previously reported related with PE, and hypoxia-induced miR-210 were also submitted to the validation phase. Only miR-28-3p was found significantly elevated in the plasma of PE patients. Compared with the level of plasma miR-28-3p of the dogs before PE, the elevated miR-28-3p did not alter significantly at 1, 2, 4 and 6h after PE. The area under the receiver operating characteristic (ROC) curve of plasma miR-28-3p was 0.792 (95% confidence interval: 0.689-0.896). KEGG pathway analysis showed that miR-28-3p might involve in PE related pathways such as inositol phosphate metabolism and phosphatidylinositol signaling system.
  
  
  CONCLUSIONS
  Our study indicated that elevated plasma miR-28-3p could be used as a non-invasive and stable biomarker in the detection of PE. Further researches on the miRNA are warranted.
authors.affiliation: Department of Oncology, First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing 210029, PR China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Department of Thoracic Surgery, First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing 210029, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wen
authors.affiliation: Department of Respiration, The Affiliated Jiangning Hospital of Nanjing Medical University, 168 Gushan Road, Nanjing 211100, PR China.
authors.forename: Xia
authors.initials: X
authors.lastname: Shan
authors.affiliation: Department of Oncology, First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing 210029, PR China.
authors.forename: Jiaqi
authors.initials: J
authors.lastname: Qian
authors.affiliation: Department of Pathology, First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing 210029, PR China.
authors.forename: Hai
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Emergency, First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing 210029, PR China.
authors.forename: Ting
authors.initials: T
authors.lastname: Jiang
authors.affiliation: Department of Emergency, First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing 210029, PR China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Gastroenterology, First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing 210029, PR China.
authors.forename: Wenfang
authors.initials: W
authors.lastname: Cheng
authors.affiliation: Department of Cardiology, First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing 210029, PR China.
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Natural Medicines and Department of Pharmacognosy, China Pharmaceutical University, No. 24 Tongjia Lane, Nanjing, 210009,PR China.
authors.forename: Lianwen
authors.initials: L
authors.lastname: Qi
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, No. 22 Hankou Road, Nanjing 210093, PR China.
authors.forename: Yin
authors.initials: Y
authors.lastname: Ding
authors.affiliation: Department of Oncology, First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing 210029, PR China; Cancer Center of Nanjing Medical University, Nanjing 210029, PR China.
authors.forename: Ping
authors.initials: P
authors.lastname: Liu
authors.affiliation: Department of Oncology, First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing 210029, PR China. Electronic address: zhuwei1983213@163.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhu
authors.affiliation: Department of Emergency, First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing 210029, PR China. Electronic address: chenyandoc@163.com.
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood; D004285:Dogs; D005260:Female; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood* / Q000235:genetics; D011655:Pulmonary Embolism / Q000097:blood* / Q000175:diagnosis* / Q000235:genetics; D012372:ROC Curve; D059467:Transcriptome
chemical_list: D015415:Biomarkers; C570628:MIRN28 microRNA, human; D035683:MicroRNAs
keywords: Biomarker; MiRNA; Plasma; Pulmonary embolism