title: Naked-eye quantitative aptamer-based assay on paper device.
abstract: This work initially describes the design of low-cost, naked-eye quantitative aptamer-based assays by using microfluidic paper-based analytical device (μPAD). Two new detection motifs are proposed for quantitative μPAD measurement without using external electronic readers, which depend on the length of colored region in a strip-like μPAD and the number of colorless detection microzones in a multi-zone μPAD. The length measuring method is based on selective color change of paper from colorless to blue-black via formation of iodine-starch complex. The counting method is conducted on the basis of oxidation-reduction reaction between hydrogen peroxide and potassium permanganate. Their utility is well demonstrated with sensitive, specific detection of adenosine as a model analyte with the naked eye in buffer samples and undiluted human serum. These equipment-free quantitative methods proposed thus hold great potential for the development of more aptamer-based assays that are simple, cost-efficient, portable, and user-friendly for various point-of-care applications particularly in resource-constrained environments.
authors.affiliation: Guangxi Key Laboratory of Electrochemical and Magnetochemical Function Materials, College of Chemistry and Bioengineering, Guilin University of Technology, Guilin 541004, PR China. Electronic address: zy@glut.edu.cn.
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Guangxi Key Laboratory of Electrochemical and Magnetochemical Function Materials, College of Chemistry and Bioengineering, Guilin University of Technology, Guilin 541004, PR China.
authors.forename: Dong
authors.initials: D
authors.lastname: Gao
authors.affiliation: Guangxi Key Laboratory of Electrochemical and Magnetochemical Function Materials, College of Chemistry and Bioengineering, Guilin University of Technology, Guilin 541004, PR China.
authors.forename: Jinlong
authors.initials: J
authors.lastname: Fan
authors.affiliation: Guangxi Key Laboratory of Electrochemical and Magnetochemical Function Materials, College of Chemistry and Bioengineering, Guilin University of Technology, Guilin 541004, PR China. Electronic address: Niejinfang@glut.edu.cn.
authors.forename: Jinfang
authors.initials: J
authors.lastname: Nie
authors.affiliation: Guangxi Key Laboratory of Electrochemical and Magnetochemical Function Materials, College of Chemistry and Bioengineering, Guilin University of Technology, Guilin 541004, PR China.
authors.forename: Shangwang
authors.initials: S
authors.lastname: Le
authors.affiliation: Guangxi Key Laboratory of Electrochemical and Magnetochemical Function Materials, College of Chemistry and Bioengineering, Guilin University of Technology, Guilin 541004, PR China.
authors.forename: Wenyuan
authors.initials: W
authors.lastname: Zhu
authors.affiliation: Guangxi Key Laboratory of Electrochemical and Magnetochemical Function Materials, College of Chemistry and Bioengineering, Guilin University of Technology, Guilin 541004, PR China.
authors.forename: Jiani
authors.initials: J
authors.lastname: Yang
authors.affiliation: Guangxi Key Laboratory of Electrochemical and Magnetochemical Function Materials, College of Chemistry and Bioengineering, Guilin University of Technology, Guilin 541004, PR China.
authors.forename: Jianping
authors.initials: J
authors.lastname: Li
mesh_terms: D000241:Adenosine / Q000097:blood* / Q000737:chemistry; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D003124:Colorimetry; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D010084:Oxidation-Reduction; D010209:Paper; D019095:Point-of-Care Systems
chemical_list: D052157:Aptamers, Nucleotide; D006861:Hydrogen Peroxide; D000241:Adenosine