title: Four receptor-like cytoplasmic kinases regulate development and immunity in rice.
abstract: Receptor-like cytoplasmic kinases (RLCKs) represent a large family of proteins in plants. However, few RLCKs have been well characterized. Here, we report the functional characterization of four rice RLCKs - OsRLCK57, OsRLCK107, OsRLCK118 and OsRLCK176 from subfamily VII. These OsRLCKs interact with the rice brassinosteroid receptor, OsBRI1 in yeast cell, but not the XA21 immune receptor. Transgenic lines silenced for each of these genes have enlarged leaf angles and are hypersensitive to brassinolide treatment compared to wild type rice. Transgenic plants silenced for OsRLCK57 had significantly fewer tillers and reduced panicle secondary branching, and lines silenced for OsRLCK107 and OsRLCK118 produce fewer seeds. Silencing of these genes decreased Xa21 gene expression and compromised XA21-mediated immunity to Xanthomonas oryzae pv. oryzae. Our study demonstrates that these OsRLCKs negatively regulate BR signalling, while positively regulating immune responses by contributing to the expression of the immune receptor XA21.
authors.affiliation: State Key Laboratory of Hybrid Rice, Key Laboratory of Major Crop Diseases & Collaborative Innovation Center for Hybrid Rice in Yangtze River Basin, Sichuan Agricultural University at Wenjiang, Chengdu, Sichuan, 611130, China.
authors.forename: Xiaogang
authors.initials: X
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Hybrid Rice, Key Laboratory of Major Crop Diseases & Collaborative Innovation Center for Hybrid Rice in Yangtze River Basin, Sichuan Agricultural University at Wenjiang, Chengdu, Sichuan, 611130, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Hybrid Rice, Key Laboratory of Major Crop Diseases & Collaborative Innovation Center for Hybrid Rice in Yangtze River Basin, Sichuan Agricultural University at Wenjiang, Chengdu, Sichuan, 611130, China.
authors.forename: Chunfang
authors.initials: C
authors.lastname: Peng
authors.affiliation: State Key Laboratory of Hybrid Rice, Key Laboratory of Major Crop Diseases & Collaborative Innovation Center for Hybrid Rice in Yangtze River Basin, Sichuan Agricultural University at Wenjiang, Chengdu, Sichuan, 611130, China.
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Hybrid Rice, Key Laboratory of Major Crop Diseases & Collaborative Innovation Center for Hybrid Rice in Yangtze River Basin, Sichuan Agricultural University at Wenjiang, Chengdu, Sichuan, 611130, China.
authors.forename: Junjie
authors.initials: J
authors.lastname: Yin
authors.affiliation: State Key Laboratory of Hybrid Rice, Key Laboratory of Major Crop Diseases & Collaborative Innovation Center for Hybrid Rice in Yangtze River Basin, Sichuan Agricultural University at Wenjiang, Chengdu, Sichuan, 611130, China.
authors.forename: Weitao
authors.initials: W
authors.lastname: Li
authors.affiliation: State Key Laboratory of Hybrid Rice, Key Laboratory of Major Crop Diseases & Collaborative Innovation Center for Hybrid Rice in Yangtze River Basin, Sichuan Agricultural University at Wenjiang, Chengdu, Sichuan, 611130, China.
authors.forename: Min
authors.initials: M
authors.lastname: He
authors.affiliation: State Key Laboratory of Hybrid Rice, Key Laboratory of Major Crop Diseases & Collaborative Innovation Center for Hybrid Rice in Yangtze River Basin, Sichuan Agricultural University at Wenjiang, Chengdu, Sichuan, 611130, China.
authors.forename: Jichun
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Plant Pathology and the Genome Center, University of California, Davis, CA, 95616, USA.
authors.forename: Mawsheng
authors.initials: M
authors.lastname: Chern
authors.affiliation: State Key Laboratory of Hybrid Rice, Key Laboratory of Major Crop Diseases & Collaborative Innovation Center for Hybrid Rice in Yangtze River Basin, Sichuan Agricultural University at Wenjiang, Chengdu, Sichuan, 611130, China.
authors.forename: Can
authors.initials: C
authors.lastname: Yuan
authors.affiliation: State Key Laboratory of Hybrid Rice, Key Laboratory of Major Crop Diseases & Collaborative Innovation Center for Hybrid Rice in Yangtze River Basin, Sichuan Agricultural University at Wenjiang, Chengdu, Sichuan, 611130, China.
authors.forename: Wenguan
authors.initials: W
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Hybrid Rice, Key Laboratory of Major Crop Diseases & Collaborative Innovation Center for Hybrid Rice in Yangtze River Basin, Sichuan Agricultural University at Wenjiang, Chengdu, Sichuan, 611130, China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Hybrid Rice, Key Laboratory of Major Crop Diseases & Collaborative Innovation Center for Hybrid Rice in Yangtze River Basin, Sichuan Agricultural University at Wenjiang, Chengdu, Sichuan, 611130, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Qin
authors.affiliation: State Key Laboratory of Hybrid Rice, Key Laboratory of Major Crop Diseases & Collaborative Innovation Center for Hybrid Rice in Yangtze River Basin, Sichuan Agricultural University at Wenjiang, Chengdu, Sichuan, 611130, China.
authors.forename: Bintian
authors.initials: B
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Hybrid Rice, Key Laboratory of Major Crop Diseases & Collaborative Innovation Center for Hybrid Rice in Yangtze River Basin, Sichuan Agricultural University at Wenjiang, Chengdu, Sichuan, 611130, China.
authors.forename: Xianjun
authors.initials: X
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Hybrid Rice, Key Laboratory of Major Crop Diseases & Collaborative Innovation Center for Hybrid Rice in Yangtze River Basin, Sichuan Agricultural University at Wenjiang, Chengdu, Sichuan, 611130, China.
authors.forename: Shigui
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Plant Pathology and the Genome Center, University of California, Davis, CA, 95616, USA.
authors.forename: Pamela
authors.initials: P
authors.lastname: Ronald
authors.affiliation: State Key Laboratory of Hybrid Rice, Key Laboratory of Major Crop Diseases & Collaborative Innovation Center for Hybrid Rice in Yangtze River Basin, Sichuan Agricultural University at Wenjiang, Chengdu, Sichuan, 611130, China.
authors.forename: Xuewei
authors.initials: X
authors.lastname: Chen
mesh_terms: D060467:Disease Resistance / Q000502:physiology; D020868:Gene Silencing / Q000502:physiology; D012275:Oryza / Q000201:enzymology / Q000254:growth & development* / Q000276:immunology; D010802:Phylogeny; D010940:Plant Proteins / Q000502:physiology*; D030821:Plants, Genetically Modified; D015398:Signal Transduction / Q000502:physiology; D013312:Stress, Physiological; D014974:Xanthomonas
chemical_list: D010940:Plant Proteins
keywords: OsRLCK; pattern recognition receptor