title: Metabolic Engineering of TCA Cycle for Production of Chemicals.
abstract: The tricarboxylic acid (TCA) cycle has been used for decades in the microbial production of chemicals such as citrate, L-glutamate, and succinate. Maximizing yield is key for cost-competitive production. However, for most TCA cycle products, the maximum pathway yield is lower than the theoretical maximum yield (Y(E)). For succinate, this was solved by creating two pathways to the product, using both branches of the TCA cycle, connected by the glyoxylate shunt (GS). A similar solution cannot be applied directly for production of compounds from the oxidative branch of the TCA cycle because irreversible reactions are involved. Here, we describe how this can be overcome and what the impact is on the yield.
authors.affiliation: Bioprocess Engineering, Wageningen University, Wageningen UR PO Box 16, 6700AA, Wageningen, The Netherlands.
authors.forename: Kiira S
authors.initials: KS
authors.lastname: Vuoristo
authors.affiliation: Biobased Products, Wageningen University and Research Centre, Wageningen, The Netherlands.
authors.forename: Astrid E
authors.initials: AE
authors.lastname: Mars
authors.affiliation: Biobased Products, Wageningen University and Research Centre, Wageningen, The Netherlands.
authors.forename: Johan P M
authors.initials: JPM
authors.lastname: Sanders
authors.affiliation: Bioprocess Engineering, Wageningen University, Wageningen UR PO Box 16, 6700AA, Wageningen, The Netherlands; Biobased Products, Wageningen University and Research Centre, Wageningen, The Netherlands.
authors.forename: Gerrit
authors.initials: G
authors.lastname: Eggink
authors.affiliation: Bioprocess Engineering, Wageningen University, Wageningen UR PO Box 16, 6700AA, Wageningen, The Netherlands. Electronic address: ruud.weusthuis@wur.nl.
authors.forename: Ruud A
authors.initials: RA
authors.lastname: Weusthuis
mesh_terms: D002952:Citric Acid Cycle; D004926:Escherichia coli; D060847:Metabolic Engineering; D019802:Succinic Acid / Q000378:metabolism*
chemical_list: D019802:Succinic Acid
keywords: CO(2) fixation; citric acid; glutamic acid; glyoxylate shunt; metabolic engineering; succinic acid; tricarboxylic acid cycle