title: Isolation and experimental inoculation of an S INDEL strain of porcine epidemic diarrhea virus in Japan.
abstract: In 2013, porcine epidemic diarrhea (PED) was reported in Japan for the first time in 7years and caused significant economic losses. In the present study, we isolated PED virus (PEDV) circulating in Japan using Vero cell cultures and analyzed sequences of S1 genes of these PEDV isolates. Sequence analysis revealed that one of these strains contained distinct insertion and deletions in the S gene (i.e., S INDEL). Furthermore, inoculation of PEDV into 1-week-old pigs demonstrated that the S INDEL strain had a lower pathogenicity than the North American (NA) prototype strain. This is the first report comparing pathogenicity of an S INDEL strain with the NA prototype strain following experimental inoculation. Excretion of PEDV in the feces of S INDEL strain-inoculated pigs occurred later than in NA prototype strain-inoculated pigs. Thus, our findings suggested that the S INDEL strain had different viral dynamics than the NA prototype strain.
authors.affiliation: Research and Development Section, Zen-noh Institute of Animal Health, Chiba 285-0043, Japan. Electronic address: yamamoto-ryuuichi@zennoh.or.jp.
authors.forename: Ryuichi
authors.initials: R
authors.lastname: Yamamoto
authors.affiliation: Research and Development Section, Zen-noh Institute of Animal Health, Chiba 285-0043, Japan.
authors.forename: Junichi
authors.initials: J
authors.lastname: Soma
authors.affiliation: Research and Development Section, Zen-noh Institute of Animal Health, Chiba 285-0043, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Nakanishi
authors.affiliation: Research and Development Section, Zen-noh Institute of Animal Health, Chiba 285-0043, Japan.
authors.forename: Ryosaku
authors.initials: R
authors.lastname: Yamaguchi
authors.affiliation: Research and Development Section, Zen-noh Institute of Animal Health, Chiba 285-0043, Japan.
authors.forename: Shingo
authors.initials: S
authors.lastname: Niinuma
mesh_terms: D000818:Animals; D001483:Base Sequence; D002522:Chlorocebus aethiops; D018352:Coronavirus Infections / Q000662:veterinary* / Q000821:virology; D005243:Feces / Q000821:virology; D054643:INDEL Mutation; D007421:Intestine, Small / Q000821:virology; D007564:Japan; D008969:Molecular Sequence Data; D010802:Phylogeny; D053485:Porcine epidemic diarrhea virus / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology*; D064370:Spike Glycoprotein, Coronavirus / Q000235:genetics* / Q000378:metabolism; D013552:Swine; D013553:Swine Diseases / Q000821:virology*; D014709:Vero Cells; D014774:Virulence
chemical_list: D064370:Spike Glycoprotein, Coronavirus
keywords: Pathogenicity; Porcine epidemic diarrhea; S INDEL; Viral dynamics