title: Structure of the mouse Toll-like receptor 13 ectodomain in complex with a conserved sequence from bacterial 23S ribosomal RNA.
abstract: Toll-like receptor 13 (TLR13) recognizes a conserved 10-nucleotide sequence from bacterial 23S ribosomal RNA, and binding of TLR13 to the target rRNA molecule triggers immune responses. Recently, the crystal structure of the TLR13 ectodomain bound by a 13-nucleotide single-stranded RNA (ssRNA13) was determined by using the initial phases provided by a cryo-electron microscopy map of TLR13 in complex with a 25-nucleotide ssRNA (ssRNA25). This structural snapshot describes a unique method for solving the crystal structure of the TLR13-ssRNA13 complex based on medium-resolution reconstruction of TLR13-ssRNA25 cryo-electron microscopy images.
authors.affiliation: State Key Laboratory of Membrane Biology, School of Life Sciences, Tsinghua University, Beijing, China.|Center for Structural Biology, School of Life Sciences, Tsinghua University, Beijing, China.
authors.forename: Jiawei
authors.initials: J
authors.lastname: Wang
authors.affiliation: Center for Structural Biology, School of Life Sciences, Tsinghua University, Beijing, China.|Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, China.|Ministry of Education Key Laboratory of Protein Science, School of Life Sciences, Tsinghua University, Beijing, China.
authors.forename: Jijie
authors.initials: J
authors.lastname: Chai
authors.affiliation: Center for Structural Biology, School of Life Sciences, Tsinghua University, Beijing, China.|Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, China.|Ministry of Education Key Laboratory of Protein Science, School of Life Sciences, Tsinghua University, Beijing, China.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Wang
mesh_terms: D000818:Animals; D001665:Binding Sites; D017124:Conserved Sequence; D020285:Cryoelectron Microscopy; D018360:Crystallography, X-Ray; D051379:Mice; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D011119:Polynucleotides; D011485:Protein Binding; D054730:Protein Interaction Domains and Motifs; D017433:Protein Structure, Secondary; D012329:RNA, Bacterial / Q000737:chemistry* / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000737:chemistry* / Q000235:genetics; D051193:Toll-Like Receptors / Q000737:chemistry* / Q000235:genetics
chemical_list: D011119:Polynucleotides; D012329:RNA, Bacterial; D012338:RNA, Ribosomal, 23S; C539093:Tlr13 protein, mouse; D051193:Toll-Like Receptors
keywords: 23S rRNA; Toll-like receptor 13; cryo-electron microscopy; molecular replacement; phase extension