title: CISD1 in association with obesity-associated dysfunctional adipogenesis in human visceral adipose tissue.
abstract: OBJECTIVE
  To investigate CISD1 mRNA and protein in human adipose tissue in association with obesity and adipogenesis.
  
  
  METHODS
  Subcutaneous (SAT) and visceral (VAT) adipose tissue CISD1 gene expression (real-time PCR) and protein (Western blot) levels were investigated in human adipose tissue and during human adipocyte differentiation.
  
  
  RESULTS
  SAT and VAT CISD1 mRNA and protein levels were significantly decreased in subjects with obesity and negatively correlated with BMI after controlling for age and sex. In participants with morbid obesity, VAT CISD1 gene expression was positively correlated with insulin sensitivity (r = 0.47, P = 0.01), and bariatric surgery-induced weight loss led to increased SAT CISD1 mRNA levels. In both VAT and SAT, CISD1 gene expression was significantly associated with SIRT1, ISCA2, and mitochondrial biogenesis-related (PPARGC1A, TFAM, and MT-CO3) and browning-related (PRDM16 and UCP1) gene expression. In addition, VAT CISD1 gene expression was significantly associated with adipogenic and iron metabolism-related genes. Importantly, these correlations were replicated in a second cohort. At the cellular level, CISD1 gene expression increased during human adipocyte differentiation in correlation with adipogenic genes (r > 0.60, P < 0.005).
  
  
  CONCLUSIONS
  These data suggest a possible role of CISD1 in obesity-associated dysfunctional adipogenesis in human VAT.
authors.affiliation: Department of Diabetes, Endocrinology and Nutrition, Institut D'investigació Biomèdica De Girona (IdIBGi), CIBEROBN (CB06/03/010) and Instituto De Salud Carlos III (ISCIII), Girona, Spain.
authors.forename: José María
authors.initials: JM
authors.lastname: Moreno-Navarrete
authors.affiliation: Department of Diabetes, Endocrinology and Nutrition, Institut D'investigació Biomèdica De Girona (IdIBGi), CIBEROBN (CB06/03/010) and Instituto De Salud Carlos III (ISCIII), Girona, Spain.
authors.forename: María
authors.initials: M
authors.lastname: Moreno
authors.affiliation: Department of Diabetes, Endocrinology and Nutrition, Institut D'investigació Biomèdica De Girona (IdIBGi), CIBEROBN (CB06/03/010) and Instituto De Salud Carlos III (ISCIII), Girona, Spain.
authors.forename: Francisco
authors.initials: F
authors.lastname: Ortega
authors.affiliation: Department of Diabetes, Endocrinology and Nutrition, Institut D'investigació Biomèdica De Girona (IdIBGi), CIBEROBN (CB06/03/010) and Instituto De Salud Carlos III (ISCIII), Girona, Spain.
authors.forename: Mònica
authors.initials: M
authors.lastname: Sabater
authors.affiliation: Department of Diabetes, Endocrinology and Nutrition, Institut D'investigació Biomèdica De Girona (IdIBGi), CIBEROBN (CB06/03/010) and Instituto De Salud Carlos III (ISCIII), Girona, Spain.
authors.forename: Gemma
authors.initials: G
authors.lastname: Xifra
authors.affiliation: Department of Diabetes, Endocrinology and Nutrition, Institut D'investigació Biomèdica De Girona (IdIBGi), CIBEROBN (CB06/03/010) and Instituto De Salud Carlos III (ISCIII), Girona, Spain.
authors.forename: Wifredo
authors.initials: W
authors.lastname: Ricart
authors.affiliation: Department of Diabetes, Endocrinology and Nutrition, Institut D'investigació Biomèdica De Girona (IdIBGi), CIBEROBN (CB06/03/010) and Instituto De Salud Carlos III (ISCIII), Girona, Spain.
authors.forename: José Manuel
authors.initials: JM
authors.lastname: Fernández-Real
mesh_terms: D050156:Adipogenesis / Q000235:genetics*; D000273:Adipose Tissue / Q000378:metabolism; D000328:Adult; D000368:Aged; D050110:Bariatric Surgery; D015992:Body Mass Index; D002454:Cell Differentiation / Q000235:genetics; D002478:Cells, Cultured; D015331:Cohort Studies; D005260:Female; D015870:Gene Expression; D006801:Humans; D007333:Insulin Resistance / Q000235:genetics; D050152:Intra-Abdominal Fat / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D024101:Mitochondrial Proteins / Q000235:genetics* / Q000378:metabolism; D009765:Obesity / Q000235:genetics* / Q000378:metabolism / Q000473:pathology / Q000601:surgery
chemical_list: C525595:CISD1 protein, human; D024101:Mitochondrial Proteins