title: A Fluorescent Tile DNA Diagnocode System for In Situ Rapid and Selective Diagnosis of Cytosolic RNA Cancer Markers.
abstract: Accurate cancer diagnosis often requires extraction and purification of genetic materials from cells, and sophisticated instrumentations that follow. Otherwise in order to directly treat the diagnostic materials to cells, multiple steps to optimize dose concentration and treatment time are necessary due to diversity in cellular behaviors. These processes may offer high precision but hinder fast analysis of cancer, especially in clinical situations that need rapid detection and characterization of cancer. Here we present a novel fluorescent tile DNA nanostructure delivered to cancer cytosol by employing nanoparticle technology. Its structural anisotropicity offers easy manipulation for multifunctionalities, enabling the novel DNA nanostructure to detect intracellular cancer RNA markers with high specificity within 30 minutes post treatment, while the nanoparticle property bypasses the requirement of treatment optimization, effectively reducing the complexity of applying the system for cancer diagnosis. Altogether, the system offers a precise and rapid detection of cancer, suggesting the future use in the clinical fields.
authors.affiliation: School of Chemical Engineering, Sungkyunkwan University, Suwon, Gyeonggi-do, 440-746, South Korea.|Department of Chemical and Biomolecular Engineering, Sogang University, Seoul, 121-742, South Korea.
authors.forename: Kyung Soo
authors.initials: KS
authors.lastname: Park
authors.affiliation: School of Chemical Engineering, Sungkyunkwan University, Suwon, Gyeonggi-do, 440-746, South Korea.
authors.forename: Seung Won
authors.initials: SW
authors.lastname: Shin
authors.affiliation: School of Chemical Engineering, Sungkyunkwan University, Suwon, Gyeonggi-do, 440-746, South Korea.
authors.forename: Min Su
authors.initials: MS
authors.lastname: Jang
authors.affiliation: School of Chemical Engineering, Sungkyunkwan University, Suwon, Gyeonggi-do, 440-746, South Korea.
authors.forename: Woojung
authors.initials: W
authors.lastname: Shin
authors.affiliation: Department of Biotechnology, Yonsei University, Seoul, 120-749, South Korea.
authors.forename: Kisuk
authors.initials: K
authors.lastname: Yang
authors.affiliation: School of Integrative Engineering, Chung-Ang University, Seoul, 156-756, South Korea.
authors.forename: Junhong
authors.initials: J
authors.lastname: Min
authors.affiliation: Department of Biotechnology, Yonsei University, Seoul, 120-749, South Korea.
authors.forename: Seung-Woo
authors.initials: SW
authors.lastname: Cho
authors.affiliation: Department of Chemical and Biomolecular Engineering, Sogang University, Seoul, 121-742, South Korea.
authors.forename: Byung-Keun
authors.initials: BK
authors.lastname: Oh
authors.affiliation: School of Chemical Engineering, Sungkyunkwan University, Suwon, Gyeonggi-do, 440-746, South Korea.
authors.forename: Jong Wook
authors.initials: JW
authors.lastname: Bae
authors.affiliation: Department of Engineering Science and Mechanics, Virginia Tech, Blacksburg, VA 24061, USA.
authors.forename: Sunghwan
authors.initials: S
authors.lastname: Jung
authors.affiliation: Department of Chemical and Biomolecular Engineering, Sogang University, Seoul, 121-742, South Korea.
authors.forename: Jeong-Woo
authors.initials: JW
authors.lastname: Choi
authors.affiliation: School of Chemical Engineering, Sungkyunkwan University, Suwon, Gyeonggi-do, 440-746, South Korea.|SKKU Advanced Institute of Nanotechnology (SAINT), Sungkyunkwan University, Suwon, Gyeonggi-do, 440-746, South Korea.
authors.forename: Soong Ho
authors.initials: SH
authors.lastname: Um
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis / Q000235:genetics; D003600:Cytosol / Q000378:metabolism*; D004247:DNA / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D046529:Microscopy, Electron, Transmission; D049329:Nanostructures / Q000737:chemistry*; D009369:Neoplasms / Q000378:metabolism / Q000473:pathology; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012313:RNA / Q000032:analysis* / Q000378:metabolism
chemical_list: D014408:Biomarkers, Tumor; D005456:Fluorescent Dyes; D012313:RNA; D004247:DNA