title: Role of gga-miR-221 and gga-miR-222 during Tumour Formation in Chickens Infected by Subgroup J Avian Leukosis Virus.
abstract: Subgroup J avian leukosis virus (ALV-J) causes a neoplastic disease in infected chickens. Differential expression patterns of microRNAs (miRNAs) are closely related to the formation and growth of tumors. (1) BACKGROUND: This study was undertaken to understand how miRNAs might be related to tumor growth during ALV-J infection. We chose to characterize the effects of miR-221 and miR-222 on cell proliferation, migration, and apoptosis based on previous microarray data. (2) METHODS: In vivo, the expression levels of miR-221 and miR-222 were significantly increased in the liver of ALV-J infected chickens (p < 0.01). Over-expression of gga-miR-221 and gga-miR-222 promoted the proliferation, migration, and growth of DF-1 cells, and decreased the expression of BCL-2 modifying factor (BMF) making cells more resistant to apoptosis. (3) RESULTS: Our results suggest that gga-miR-221 and gga-miR-222 may be tumour formation relevant gene in chicken that promote proliferation, migration, and growth of cancer cells, and inhibit apoptosis. BMF expression was significantly reduced in vivo 70 days after ALV-J infection. They may also play a pivotal role in tumorigenesis during ALV-J infection.
authors.affiliation: College of Animal Science, South China Agricultural University &amp; Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, Guangzhou 510642, China. daizhenkai@stu.scau.edu.cn.
authors.forename: Zhenkai
authors.initials: Z
authors.lastname: Dai
authors.affiliation: China-UK-NYNU-RRes Joint laboratory of Insect Biology, Nanyang Normal Universiy, Nanyang 473000, China. jijun84@gmail.com.
authors.forename: Jun
authors.initials: J
authors.lastname: Ji
authors.affiliation: College of Animal Science, South China Agricultural University &amp; Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, Guangzhou 510642, China. wuliaozhiji404@163.com.
authors.forename: Yiming
authors.initials: Y
authors.lastname: Yan
authors.affiliation: College of Animal Science, South China Agricultural University &amp; Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, Guangzhou 510642, China. Linwencheng@cau.edu.cn.
authors.forename: Wencheng
authors.initials: W
authors.lastname: Lin
authors.affiliation: College of Animal Science, South China Agricultural University &amp; Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, Guangzhou 510642, China. lihongxing12@126.com.|Institute of Animal Science, Guangdong Academy of Agriculture Sciences, Guangzhou 510640, China. lihongxing12@126.com.
authors.forename: Hongxin
authors.initials: H
authors.lastname: Li
authors.affiliation: College of Animal Science, South China Agricultural University &amp; Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, Guangzhou 510642, China. fengch@scau.edu.cn.|Institute of Animal Science, Guangdong Academy of Agriculture Sciences, Guangzhou 510640, China. fengch@scau.edu.cn.|Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou 510642, China. fengch@scau.edu.cn.|South China Collaborative Innovation Center for Poultry Disease Control and Product Safety, Guangzhou 510640, China. fengch@scau.edu.cn.
authors.forename: Feng
authors.initials: F
authors.lastname: Chen
authors.affiliation: College of Animal Science, South China Agricultural University &amp; Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, Guangzhou 510642, China. 15813372565@163.coml.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: College of Animal Science, South China Agricultural University &amp; Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, Guangzhou 510642, China. Wgchen81@scau.edu.cn.|Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou 510642, China. Wgchen81@scau.edu.cn.|South China Collaborative Innovation Center for Poultry Disease Control and Product Safety, Guangzhou 510640, China. Wgchen81@scau.edu.cn.
authors.forename: Weiguo
authors.initials: W
authors.lastname: Chen
authors.affiliation: College of Animal Science, South China Agricultural University &amp; Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, Guangzhou 510642, China. yizbi@126.com.|Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou 510642, China. yizbi@126.com.|South China Collaborative Innovation Center for Poultry Disease Control and Product Safety, Guangzhou 510640, China. yizbi@126.com.
authors.forename: Yingzuo
authors.initials: Y
authors.lastname: Bi
authors.affiliation: College of Animal Science, South China Agricultural University &amp; Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, Guangzhou 510642, China. qmx@scau.edu.cn.|China-UK-NYNU-RRes Joint laboratory of Insect Biology, Nanyang Normal Universiy, Nanyang 473000, China. qmx@scau.edu.cn.|Institute of Animal Science, Guangdong Academy of Agriculture Sciences, Guangzhou 510640, China. qmx@scau.edu.cn.|Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou 510642, China. qmx@scau.edu.cn.|South China Collaborative Innovation Center for Poultry Disease Control and Product Safety, Guangzhou 510640, China. qmx@scau.edu.cn.
authors.forename: Qingmei
authors.initials: Q
authors.lastname: Xie
mesh_terms: D000818:Animals; D017209:Apoptosis; D001353:Avian Leukosis / Q000473:pathology* / Q000821:virology*; D001354:Avian Leukosis Virus / Q000254:growth & development*; D063646:Carcinogenesis; D002460:Cell Line; D002465:Cell Movement; D049109:Cell Proliferation; D002645:Chickens; D020869:Gene Expression Profiling; D054884:Host-Pathogen Interactions; D008099:Liver / Q000473:pathology; D035683:MicroRNAs / Q000378:metabolism*
chemical_list: D035683:MicroRNAs
keywords: BMF; apoptosis; cancer; cisplatin; doxorubicin; gga-miR-221; gga-miR-222; subgroup J avian leukosis virus