title: Tuning cellular responses to BMP-2 with material surfaces.
abstract: Bone morphogenetic protein 2 (BMP-2) has been known for decades as a strong osteoinductive factor and for clinical applications is combined solely with collagen as carrier material. The growing concerns regarding side effects and the importance of BMP-2 in several developmental and physiological processes have raised the need to improve the design of materials by controlling BMP-2 presentation. Inspired by the natural cell environment, new material surfaces have been engineered and tailored to provide both physical and chemical cues that regulate BMP-2 activity. Here we describe surfaces designed to present BMP-2 to cells in a spatially and temporally controlled manner. This is achieved by trapping BMP-2 using physicochemical interactions, either covalently grafted or combined with other extracellular matrix components. In the near future, we anticipate that material science and biology will integrate and further develop tools for in vitro studies and potentially bring some of them toward in vivo applications.
authors.affiliation: Department of New Materials and Biosystems, Max Planck Institute for Intelligent Systems, Heisenbergstr. 3, D-70569 Stuttgart, Germany.|Department of Biophysical Chemistry, University of Heidelberg, INF 253, D-69120 Heidelberg, Germany, Tel: +49-6221-54 5064.
authors.forename: Elisa
authors.initials: E
authors.lastname: Migliorini
authors.affiliation: CNRS-UMR 5628, LMGP, 3 parvis L.Néel, F-38 016 Grenoble, France.|University Grenoble Alpes, Grenoble Institute of Technology, LMGP, 3 parvis Louis Néel, F-28016 Grenoble, France.|INSERM U823, ERL CNRS5284, Université de Grenoble Alpes, Institut Albert Bonniot, Site Santé, BP170, 38042 Grenoble cedex 9, France, Tel: +33-04-56529311.
authors.forename: Anne
authors.initials: A
authors.lastname: Valat
authors.affiliation: CNRS-UMR 5628, LMGP, 3 parvis L.Néel, F-38 016 Grenoble, France.|University Grenoble Alpes, Grenoble Institute of Technology, LMGP, 3 parvis Louis Néel, F-28016 Grenoble, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Picart
authors.affiliation: Department of New Materials and Biosystems, Max Planck Institute for Intelligent Systems, Heisenbergstr. 3, D-70569 Stuttgart, Germany.|Department of Biophysical Chemistry, University of Heidelberg, INF 253, D-69120 Heidelberg, Germany, Tel: +49-6221-54 5064.
authors.forename: Elisabetta Ada
authors.initials: EA
authors.lastname: Cavalcanti-Adam
mesh_terms: D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000737:chemistry* / Q000235:genetics / Q000493:pharmacokinetics / Q000494:pharmacology; D003094:Collagen / Q000737:chemistry* / Q000493:pharmacokinetics / Q000494:pharmacology; D005109:Extracellular Matrix / Q000737:chemistry* / Q000235:genetics; D006801:Humans; D015202:Protein Engineering; D013499:Surface Properties
chemical_list: C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D003094:Collagen
keywords: BMP receptors; BMP-2; Cell adhesion; Growth factor immobilization; Material surface; Signaling