title: Therapeutic effects of curcumin on the functional disturbances and oxidative stress induced by renal ischemia/reperfusion in rats.
abstract: OBJECTIVE
  Curcumin has anti-inflammatory and antioxidative properties. The objective of this study was to investigate the therapeutic effects of curcumin on functional disturbances, oxidative stress, and leukocyte infiltration induced by renal ischemia/reperfusion (I/R).
  
  
  METHODS
  Animals were randomly divided into 9 groups. The groups with 24-h reperfusion consisted of sham-24h, I/R-24h, and three I/R groups treated with curcumin at 10, 20, or 30 mg kg(-1), i.p. after the ischemic period. The 72-h reperfusion groups also included Sham-72h, I/R-72h, I/R treated with curcumin at single dose of 20 mg kg(-1), i.p., and I/R group which received three doses of curcumin at 20 mg kg(-1), i.p., consecutively. Renal functional injury was assessed by measuring serum creatinine and urea-nitrogen concentrations. Oxidative stress was evaluated by assessment tissue malondialdehyde (MDA) and the ferric reducing/antioxidant power (FRAP) levels. Moreover, renal tissue leukocyte infiltration was measured by histopathology examination.
  
  
  RESULTS
  Ischemia/reperfusion resulted in a significant increase in serum concentration of creatinine, urea-nitrogen, tissue MDA level, and leukocytes infiltration as well as reduced FRAP level. Treatment with curcumin in 24-h reperfusion groups could only lead to a significant change in the levels of MDA and FRAP. However, in 72-h reperfusion groups, curcumin was able to correct all functional disturbances, oxidative stress, and leukocytes infiltration with more effectiveness in groups that received three doses of curcumin.
  
  
  CONCLUSIONS
  The administration of curcumin during 72-h reperfusion following 30 minutes of ischemia can decrease renal oxidative stress and leukocytes infiltration as well as improve kidney function. However, during first 24-h reperfusion, curcumin only decreased oxidative stress.
authors.affiliation: Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran.
authors.forename: Houshang
authors.initials: H
authors.lastname: Najafi
authors.affiliation: Department of Physiology, School of Medicine, Arak University of Medical Sciences, Arak, Iran.
authors.forename: Saeed
authors.initials: S
authors.lastname: Changizi Ashtiyani
authors.affiliation: Department of Nephrology, Emam Reza Hospital, Kermanshah University of Medical Sciences, Kermanshah, Iran.
authors.forename: Sayed Abolhasan
authors.initials: SA
authors.lastname: Sayedzadeh
authors.affiliation: Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran.
authors.forename: Zeynab
authors.initials: Z
authors.lastname: Mohamadi Yarijani
authors.affiliation: School of pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran.
authors.forename: Sajad
authors.initials: S
authors.lastname: Fakhri
keywords: Acute renal failure; Curcumin; Ischemia/Reperfusion; Oxidative stress