title: Rapid sensitive analysis of IDH1 mutation in lower-grade gliomas by automated genetic typing involving a quenching probe.
abstract: The authors recently found that 80% of lower-grade gliomas (LGGs) harbored a mutation in IDH1. Intraoperative detection of the mutated IDH1 helps not only differentiate LGGs from other type of brain tumors, but determine the resection border. In the current study, the authors have applied an automated genetic typing involving a quenching probe to detect the mutated IDH1. If tumor cells with the mutated IDH1 contained 10% or more in the mixture of normal and tumor cells, the device could detect it sensitively. The intraoperative assessment of IDH1 mutation is useful in brain tumor surgeries.
authors.affiliation: a Department of Neurosurgery , Nagoya University , Nagoya , Japan.
authors.forename: Michihiro
authors.initials: M
authors.lastname: Kurimoto
authors.affiliation: a Department of Neurosurgery , Nagoya University , Nagoya , Japan.|b Department of Pathology and Tumor Biology , Kyoto University , Kyoto , Japan.
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Suzuki
authors.affiliation: a Department of Neurosurgery , Nagoya University , Nagoya , Japan.|b Department of Pathology and Tumor Biology , Kyoto University , Kyoto , Japan.
authors.forename: Kosuke
authors.initials: K
authors.lastname: Aoki
authors.affiliation: a Department of Neurosurgery , Nagoya University , Nagoya , Japan.
authors.forename: Fumiharu
authors.initials: F
authors.lastname: Ohka
authors.affiliation: a Department of Neurosurgery , Nagoya University , Nagoya , Japan.
authors.forename: Goro
authors.initials: G
authors.lastname: Kondo
authors.affiliation: a Department of Neurosurgery , Nagoya University , Nagoya , Japan.
authors.forename: Kazuya
authors.initials: K
authors.lastname: Motomura
authors.affiliation: a Department of Neurosurgery , Nagoya University , Nagoya , Japan.
authors.forename: Kentaro
authors.initials: K
authors.lastname: Iijima
authors.affiliation: a Department of Neurosurgery , Nagoya University , Nagoya , Japan.|c Department of Neurosurgery , Mie University , Tsu , Mie , Japan.
authors.forename: Akane
authors.initials: A
authors.lastname: Yamamichi
authors.affiliation: a Department of Neurosurgery , Nagoya University , Nagoya , Japan.
authors.forename: Melissa
authors.initials: M
authors.lastname: Ranjit
authors.affiliation: a Department of Neurosurgery , Nagoya University , Nagoya , Japan.
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Wakabayashi
authors.affiliation: d Division of Hematology, Respiratory Medicine and Oncology, Department of Internal Medicine, Faculty of Medicine , Saga University , Saga , Japan.
authors.forename: Shinya
authors.initials: S
authors.lastname: Kimura
authors.affiliation: a Department of Neurosurgery , Nagoya University , Nagoya , Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Natsume
mesh_terms: D001932:Brain Neoplasms / Q000235:genetics* / Q000473:pathology*; D004252:DNA Mutational Analysis / Q000379:methods; D005910:Glioma / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology*; D006801:Humans; D007521:Isocitrate Dehydrogenase / Q000235:genetics*; D058889:Molecular Typing / Q000379:methods; D009154:Mutation; D059645:Mutation Rate; D060787:Neoplasm Grading; D020641:Polymorphism, Single Nucleotide
chemical_list: D007521:Isocitrate Dehydrogenase; C543588:IDH1 protein, human
keywords: Brain tumors; Cancer biomarkers; Gene profiling