title: Clickable and Antifouling Platform of Poly[(propargyl methacrylate)-ran-(2-methacryloyloxyethyl phosphorylcholine)] for Biosensing Applications.
abstract: A functional copolymer platform, namely, poly[(propargyl methacrylate)-ran-(2-methacryloyloxyethyl phosphorylcholine)] (PPgMAMPC), was synthesized by reversible addition-fragmentation chain-transfer polymerization. In principle, the alkyne moiety of propargyl methacrylate (PgMA) should serve as an active site for binding azide-containing molecules via a click reaction, i.e., Cu-catalyzed azide/alkyne cycloaddition (CuAAC), and 2-methacryloyloxyethyl phosphorylcholine (MPC), the hydrophilic monomeric unit, should enable the copolymer to suppress nonspecific adsorption. The copolymers were characterized using Fourier transform infrared (FTIR) and (1)H NMR spectroscopies. Thiol-terminated, PPgMAMPC-SH, obtained by aminolysis of PPgMAMPC, was immobilized on a gold-coated substrate using a "grafting to" approach via self-assembly. Azide-containing species, namely, biotin and peptide nucleic acid (PNA), were then immobilized on the alkyne-containing copolymeric platform via CuAAC. The potential use of surface-attached PPgMAMPC in biosensing applications was shown by detection of specific target molecules, i.e., streptavidin (SA) and DNA, by the developed sensing platform using a surface plasmon resonance technique. The copolymer composition strongly influenced the performance of the developed sensing platform in terms of signal-to-noise ratio in the case of the biotin-SA system and hybridization efficiency and mismatch discrimination for the PNA-DNA system.
authors.forename: Oraphan
authors.initials: O
authors.lastname: Wiarachai
authors.forename: Tirayut
authors.initials: T
authors.lastname: Vilaivan
authors.affiliation: Faculty of Chemistry, Materials and Bioengineering, Kansai University , 3-3-35 Yamate-cho, Suita-shi, Osaka 564-8680, Japan.
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Iwasaki
authors.forename: Voravee P
authors.initials: VP
authors.lastname: Hoven
mesh_terms: D001386:Azides / Q000737:chemistry; D020137:Base Pair Mismatch; D015374:Biosensing Techniques / Q000379:methods*; D001710:Biotin / Q000031:analogs & derivatives / Q000737:chemistry; D057930:Click Chemistry; D018076:DNA, Complementary / Q000032:analysis; D006046:Gold / Q000737:chemistry; D008689:Methacrylates / Q000138:chemical synthesis / Q000737:chemistry*; D020135:Peptide Nucleic Acids / Q000737:chemistry; D010767:Phosphorylcholine / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry; D019809:Streptavidin / Q000032:analysis; D020349:Surface Plasmon Resonance
chemical_list: D001386:Azides; D018076:DNA, Complementary; D008689:Methacrylates; D020135:Peptide Nucleic Acids; C000613796:poly((propargyl methacrylate)-ran-(2-methacryloyloxyethyl phosphorylcholine)); D010767:Phosphorylcholine; D001710:Biotin; D006046:Gold; D019809:Streptavidin