title: Luoyutong Treatment Promotes Functional Recovery and Neuronal Plasticity after Cerebral Ischemia-Reperfusion Injury in Rats.
abstract: Luoyutong (LYT) capsule has been used to treat cerebrovascular diseases clinically in China and is now patented and approved by the State Food and Drug Administration. In this retrospective validation study we investigated the ability of LYT to protect against cerebral ischemia-reperfusion injury in rats. Cerebral ischemia-reperfusion injury was induced by middle cerebral artery occlusion followed by reperfusion. Capsule containing LYT (high dose and medium dose) as treatment group and Citicoline Sodium as positive control treatment group were administered daily to rats 30 min after reperfusion. Treatment was continued for either 3 days or 14 days. A saline solution was administered to control animals. Behavior tests were performed after 3 and 14 days of treatment. Our findings revealed that LYT treatment improved the neurological outcome, decreased cerebral infarction volume, and reduced apoptosis. Additionally, LYT improved neural plasticity, as the expression of synaptophysin, microtubule associated protein, and myelin basic protein was upregulated by LYT treatment, while neurofilament 200 expression was reduced. Moreover, levels of brain derived neurotrophic factor and basic fibroblast growth factor were increased. Our results suggest that LYT treatment may protect against ischemic injury and improve neural plasticity.
authors.affiliation: Cerebrovascular Diseases Research Institute, Xuanwu Hospital, Capital Medical University, Ministry of Education, 45 Changchun Street, Beijing 100053, China ; Department of Traditional Chinese Medicine, Xuanwu Hospital, Capital Medical University, Ministry of Education, 45 Changchun Street, Beijing 100053, China.
authors.forename: Ning-Qun
authors.initials: NQ
authors.lastname: Wang
authors.affiliation: Dongfang Hospital, Beijing University of Chinese Medicine, Beijing 100078, China.
authors.forename: Li-Ye
authors.initials: LY
authors.lastname: Wang
authors.affiliation: Cerebrovascular Diseases Research Institute, Xuanwu Hospital, Capital Medical University, Ministry of Education, 45 Changchun Street, Beijing 100053, China ; Key Laboratory of Neurodegenerative Diseases (Capital Medical University), Ministry of Education, 45 Changchun Street, Beijing 100053, China.
authors.forename: Hai-Ping
authors.initials: HP
authors.lastname: Zhao
authors.affiliation: Cerebrovascular Diseases Research Institute, Xuanwu Hospital, Capital Medical University, Ministry of Education, 45 Changchun Street, Beijing 100053, China ; Department of Neurology, Xuanwu Hospital, Capital Medical University, Ministry of Education, 45 Changchun Street, Beijing 100053, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Liu
authors.affiliation: Cerebrovascular Diseases Research Institute, Xuanwu Hospital, Capital Medical University, Ministry of Education, 45 Changchun Street, Beijing 100053, China ; Key Laboratory of Neurodegenerative Diseases (Capital Medical University), Ministry of Education, 45 Changchun Street, Beijing 100053, China.
authors.forename: Rong-Liang
authors.initials: RL
authors.lastname: Wang
authors.affiliation: Department of Neurology, Xuanwu Hospital, Capital Medical University, Ministry of Education, 45 Changchun Street, Beijing 100053, China.
authors.forename: Jue-Xian
authors.initials: JX
authors.lastname: Song
authors.affiliation: Department of Neurology, Xuanwu Hospital, Capital Medical University, Ministry of Education, 45 Changchun Street, Beijing 100053, China.
authors.forename: Li
authors.initials: L
authors.lastname: Gao
authors.affiliation: Key Laboratory of Neurodegenerative Diseases (Capital Medical University), Ministry of Education, 45 Changchun Street, Beijing 100053, China.
authors.forename: Xun-Ming
authors.initials: XM
authors.lastname: Ji
authors.affiliation: Cerebrovascular Diseases Research Institute, Xuanwu Hospital, Capital Medical University, Ministry of Education, 45 Changchun Street, Beijing 100053, China ; Key Laboratory of Neurodegenerative Diseases (Capital Medical University), Ministry of Education, 45 Changchun Street, Beijing 100053, China.
authors.forename: Yu-Min
authors.initials: YM
authors.lastname: Luo