title: N,N,N-trimethylchitosan modified with well defined multifunctional polymer modules used as pDNA delivery vector.
abstract: A novel non-viral gene carrier based on N,N,N-trimethylchitosan (TMC) has been fabricated. First, well-defined copolymer P(PEGMA-co-DMAEMA) was synthesized through reversible addition fragmentation chain transfer (RAFT) polymerization of poly(ethylene glycol) methyl ether methacrylate (PEGMA) and N,N-(2-dimethylamino)ethyl methacrylamide (DMAEMA). Then allyl group grafting N,N,N-trimethylchitosan (Allyl-TMC) was synthesized via the reaction between allyl bromide and hydroxyl of TMC. Finally, P(PEGMA-co-DMAEMA) and folate were ordinally grafted onto Allyl-TMC to obtain TMC-g-P(PEGMA-co-DMAEMA)-FA. In comparison with pristine chitosan, TMC-g-P(PEGMA-co-DMAEMA)-FA has achieved both better water solubility and stronger pDNA packaging ability, which can contribute to improving gene transfection. Gene delivery efficiency of a series of TMC based functional polymers with different chitosan molecular weights has been tested. The results show that 20k-TMC-g-P(PEGMA-co-DMAEMA)-FA/pDNA complex at the weight ratio of 20 achieve the highest transfection efficiency in 293 T cells. This work presents a new strategy to modify chitosan efficiently as gene carrier material.
authors.affiliation: Key Laboratory of Functional Polymer Materials, Ministry of Education, Institute of Polymer Chemistry, College of Chemistry, Nankai University, Tianjin 300071, China.
authors.forename: Hongqi
authors.initials: H
authors.lastname: Ren
authors.affiliation: Key Laboratory of Functional Polymer Materials, Ministry of Education, Institute of Polymer Chemistry, College of Chemistry, Nankai University, Tianjin 300071, China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Liu
authors.affiliation: Key Laboratory of Functional Polymer Materials, Ministry of Education, Institute of Polymer Chemistry, College of Chemistry, Nankai University, Tianjin 300071, China.
authors.forename: Jixiang
authors.initials: J
authors.lastname: Yang
authors.affiliation: Key Laboratory of Functional Polymer Materials, Ministry of Education, Institute of Polymer Chemistry, College of Chemistry, Nankai University, Tianjin 300071, China.
authors.forename: Xian
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Biochemistry and Molecular Biology, College of Life Science, Nankai University, Tianjin 300071, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Department of Biochemistry and Molecular Biology, College of Life Science, Nankai University, Tianjin 300071, China.
authors.forename: Jiatong
authors.initials: J
authors.lastname: Chen
authors.affiliation: Key Laboratory of Functional Polymer Materials, Ministry of Education, Institute of Polymer Chemistry, College of Chemistry, Nankai University, Tianjin 300071, China. Electronic address: tyguo@nankai.edu.cn.
authors.forename: Tianying
authors.initials: T
authors.lastname: Guo
mesh_terms: D002460:Cell Line; D048271:Chitosan / Q000737:chemistry*; D005822:Genetic Vectors / Q000737:chemistry; D006801:Humans; D008689:Methacrylates / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*
chemical_list: D008689:Methacrylates; C118071:N-trimethyl chitosan chloride; D011108:Polymers; D048271:Chitosan; C049840:2-(dimethylamino)ethyl methacrylate
keywords: Chitosan modified method; Grafting; Multifunctional modules; TMC based gene vector