title: C22-bronchial and T7-alveolar epithelial cell lines of the immortomouse are excellent murine cell culture model systems to study pulmonary peroxisome biology and metabolism.
abstract: In pulmonary research, temperature-sensitive immortalized cell lines derived from the lung of the "immortomouse" (H-2k(b)-tsA58 transgenic mouse), such as C22 club cells and T7 alveolar epithelial cells type II (AECII), are frequently used cell culture models to study CC10 metabolism and surfactant synthesis. Even though peroxisomes are highly abundant in club cells and AECII and might fulfill important metabolic functions therein, these organelles have never been investigated in C22 and T7 cells. Therefore, we have characterized the peroxisomal compartment and its associated gene transcription in these cell lines. Our results show that peroxisomes are highly abundant in C22 and T7 cells, harboring a common set of enzymes, however, exhibiting specific differences in protein composition and gene expression patterns, similar to the ones observed in club cells and AECII in situ in the lung. C22 cells contain a lower number of larger peroxisomes, whereas T7 cells possess more numerous tubular peroxisomes, reflected also by higher levels of PEX11 proteins. Moreover, C22 cells harbor relatively higher amounts of catalase and antioxidative enzymes in distinct subcellular compartments, whereas T7 cells exhibit higher levels of ABCD3 and plasmalogen synthesizing enzymes as well as nuclear receptors of the PPAR family. This study suggest that the C22 and T7 cell lines of the immortomouse lung are useful models to study the regulation and metabolic function of the peroxisomal compartment and its alterations by paracrine factors in club cells and AECII.
authors.affiliation: Institute for Anatomy and Cell Biology, Medical Cell Biology, Justus Liebig University, Aulweg 123, 35385, Giessen, Germany.
authors.forename: Srikanth
authors.initials: S
authors.lastname: Karnati
authors.affiliation: Institute for Anatomy and Cell Biology, Medical Cell Biology, Justus Liebig University, Aulweg 123, 35385, Giessen, Germany.
authors.forename: Saranya
authors.initials: S
authors.lastname: Palaniswamy
authors.affiliation: Institute for Anatomy and Cell Biology, Medical Cell Biology, Justus Liebig University, Aulweg 123, 35385, Giessen, Germany.
authors.forename: Mohammad Rashedul
authors.initials: MR
authors.lastname: Alam
authors.affiliation: Institute for Anatomy and Cell Biology, Medical Cell Biology, Justus Liebig University, Aulweg 123, 35385, Giessen, Germany.
authors.forename: Gani
authors.initials: G
authors.lastname: Oruqaj
authors.affiliation: Division of Cellular Pneumology, Research Center Borstel, Leibniz-Center for Medicine and Biosciences, Borstel, Germany.|Department of Anesthesiology, University of Lübeck, Lübeck, Germany.
authors.forename: Cordula
authors.initials: C
authors.lastname: Stamme
authors.affiliation: Institute for Anatomy and Cell Biology, Medical Cell Biology, Justus Liebig University, Aulweg 123, 35385, Giessen, Germany. Eveline.Baumgart-Vogt@anatomie.med.uni-giessen.de.
authors.forename: Eveline
authors.initials: E
authors.lastname: Baumgart-Vogt
mesh_terms: D000818:Animals; D001980:Bronchi / Q000166:cytology*; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000166:cytology*; D005455:Fluorescent Antibody Technique; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008954:Models, Biological; D020675:Peroxisomes / Q000378:metabolism*; D011650:Pulmonary Alveoli / Q000166:cytology* / Q000378:metabolism*
keywords: AECII; C22; Club cells; Oxidative stress; Peroxisomes; T7