title: Cell-Specific Growth Inhibition of Human Cervical Cancer Cell by Recombinant Adenovirus p53 in vitro and in vivo.
abstract: OBJECTIVE
  Despite the significance of the p53 adenoviral vector in cancer gene therapy, an advanced strategy for the development of preferential tumor cell-specific delivery and the long-term persistent gene expression control of p53 are required. In this study, the time-course expression patterns of p53 and E6, on cervical cancer cells, were investigated to obtain a molecular level understanding of the cell-dependent tumor growth suppression effects of a recombinant adenovirus expressing p53, both in vitro and in vivo.
  
  
  METHODS
  The expressions of p53 and E6 in CaSki, SiHa, HeLa, HeLaS3, C33A and HT3 cervical cancer cell lines were examined. After infection with AdCMVp53, the cell growth inhibition was studied via cell count, MTT and Neutral red assays. After transfecting the AdCMVp53 and AdCMVLacZ into the cancer cells-xenografted nude mice, the anti-tumor effects were investigated for one month.
  
  
  RESULTS
  The p53 protein levels were more notably expressed in the CaSki and HeLa than in the SiHa and HeLaS3 On day 6, the p53 was only detected in the HeLaS3. In contrast, the p53 expression was highly maintained in the C33A and HT3. The E6 mRNA levels gradually decreased in only the CaSki and HeLa. The growth suppression effects also showed cell-dependent patterns, which were consistent with the reciprocal expression patterns of p53 and E6. After transfection of the AdCMVp53, into the CaSki- and SiHa-xenografted nude mice, the tumor size was remarkably decreased in the SiHa cells as compared to that in the AdCMVLacZ transfected mice, indicating cell-specific growth inhibition patterns.
  
  
  CONCLUSIONS
  The adenovirus-mediated p53 gene transfection was very effective both in vitro and in vivo. Also, the anti-tumor effects were accomplished via the differential role of p53-specific apoptotic cell death, which was dependent on the cervical cancer cell line.
authors.forename: Su Mi
authors.initials: SM
authors.lastname: Bae
authors.forename: Yong Wook
authors.initials: YW
authors.lastname: Kim
authors.forename: Joo Hee
authors.initials: JH
authors.lastname: Yoon
authors.forename: Jin Young
authors.initials: JY
authors.lastname: Yoo
authors.forename: Young Seok
authors.initials: YS
authors.lastname: Seo
authors.forename: Sang Lyun
authors.initials: SL
authors.lastname: Nam
authors.forename: Joon Mo
authors.initials: JM
authors.lastname: Lee
authors.forename: Sung Eun
authors.initials: SE
authors.lastname: Namkoong
authors.forename: Chong Kook
authors.initials: CK
authors.lastname: Kim
authors.forename: Yong Wan
authors.initials: YW
authors.lastname: Kim
authors.forename: Woong Shick
authors.initials: WS
authors.lastname: Ahn
keywords: Cervix neoplasm; Gene therapy; p53 gene