title: Fluorescence Quenching Property of C-Phycocyanin from Spirulina platensis and its Binding Efficacy with Viable Cell Components.
abstract: Phycocyanin is a natural brilliant blue colored, fluorescent protein, which is commonly present in cyanobacteria. In this study, C-phycocyanin was extracted and purified from Spirulina platensis, which are multicellular and filamentous cyanobacteria of greater importance because of its various biological and pharmacological potential. It was analyzed for its binding affinity towards blood cells, algal cells, genomic DNA of microalgae, and bacteria at different temperature and incubation time. It showed good binding affinity with these components even at low concentration of 2.5 μM. The purpose of this study was to evaluate the applicability of C-phycocyanin as a green fluorescent dye substituting carcinogenic chemical dyes.
authors.affiliation: Institute of Forensic Science, Gujarat Forensic Sciences University, DFS Campus, Gandhinagar, Gujarat, India.
authors.forename: Meenakshi B
authors.initials: MB
authors.lastname: Paswan
authors.affiliation: Institute of Forensic Science, Gujarat Forensic Sciences University, DFS Campus, Gandhinagar, Gujarat, India.
authors.forename: Meghna M
authors.initials: MM
authors.lastname: Chudasama
authors.affiliation: Department of Salt and Marine Chemicals, CSIR- Central Salt and Marine Chemical Research Institute, Bhavnagar, Gujarat, 364002, India.|AcSIR, CSIR-CSMCRI, Bhavnagar, India.
authors.forename: Madhusree
authors.initials: M
authors.lastname: Mitra
authors.affiliation: Department of Salt and Marine Chemicals, CSIR- Central Salt and Marine Chemical Research Institute, Bhavnagar, Gujarat, 364002, India.
authors.forename: Khushbu
authors.initials: K
authors.lastname: Bhayani
authors.affiliation: Department of Botany, CMS College, Kottayam, Kerala, Pin-686001, India.
authors.forename: Basil
authors.initials: B
authors.lastname: George
authors.affiliation: Department of Salt and Marine Chemicals, CSIR- Central Salt and Marine Chemical Research Institute, Bhavnagar, Gujarat, 364002, India.
authors.forename: Shruti
authors.initials: S
authors.lastname: Chatterjee
authors.affiliation: Department of Salt and Marine Chemicals, CSIR- Central Salt and Marine Chemical Research Institute, Bhavnagar, Gujarat, 364002, India. smishra@csmcri.org.
authors.forename: Sandhya
authors.initials: S
authors.lastname: Mishra
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000378:metabolism; D002417:Cattle; D022082:Cellular Structures / Q000378:metabolism*; D004269:DNA, Bacterial / Q000378:metabolism; D004912:Erythrocytes / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism; D005453:Fluorescence; D016680:Genome, Bacterial; D008214:Lymphocytes / Q000378:metabolism; D058086:Microalgae / Q000378:metabolism; D010798:Phycocyanin / Q000737:chemistry* / Q000378:metabolism*; D053545:Spirulina / Q000378:metabolism*
chemical_list: D004269:DNA, Bacterial; D010798:Phycocyanin
keywords: C-phycocyanin (C-PC); Fluorescence; Microalgae; Nucleic acid; Spirulina platensis