title: Simultaneous Scanning Ion Conductance Microscopy and Atomic Force Microscopy with Microchanneled Cantilevers.
abstract: We combined scanning ion conductance microscopy (SICM) and atomic force microscopy (AFM) into a single tool using AFM cantilevers with an embedded microchannel flowing into the nanosized aperture at the apex of the hollow pyramid. An electrode was positioned in the AFM fluidic circuit connected to a second electrode in the bath. We could thus simultaneously measure the ionic current and the cantilever bending (in optical beam deflection mode). First, we quantitatively compared the SICM and AFM contact points on the approach curves. Second, we estimated where the probe in SICM mode touches the sample during scanning on a calibration grid and applied the finding to image a network of neurites on a Petri dish. Finally, we assessed the feasibility of a double controller using both the ionic current and the deflection as input signals of the piezofeedback. The experimental data were rationalized in the framework of finite elements simulations.
authors.affiliation: Laboratory of Biosensors and Bioelectronics, Institute for Biomedical Engineering, ETH Zurich, CH-8092 Zurich, Switzerland.
authors.forename: Dario
authors.initials: D
authors.lastname: Ossola
authors.affiliation: Laboratory of Biosensors and Bioelectronics, Institute for Biomedical Engineering, ETH Zurich, CH-8092 Zurich, Switzerland.
authors.forename: Livie
authors.initials: L
authors.lastname: Dorwling-Carter
authors.affiliation: Laboratory of Biosensors and Bioelectronics, Institute for Biomedical Engineering, ETH Zurich, CH-8092 Zurich, Switzerland.
authors.forename: Harald
authors.initials: H
authors.lastname: Dermutz
authors.affiliation: Laboratory of Biosensors and Bioelectronics, Institute for Biomedical Engineering, ETH Zurich, CH-8092 Zurich, Switzerland.
authors.forename: Pascal
authors.initials: P
authors.lastname: Behr
authors.affiliation: Laboratory of Biosensors and Bioelectronics, Institute for Biomedical Engineering, ETH Zurich, CH-8092 Zurich, Switzerland.
authors.forename: János
authors.initials: J
authors.lastname: Vörös
authors.affiliation: Laboratory of Biosensors and Bioelectronics, Institute for Biomedical Engineering, ETH Zurich, CH-8092 Zurich, Switzerland.
authors.forename: Tomaso
authors.initials: T
authors.lastname: Zambelli
mesh_terms: D000818:Animals; D006624:Hippocampus / Q000648:ultrastructure; D018625:Microscopy, Atomic Force / Q000295:instrumentation* / Q000379:methods*; D020527:Microscopy, Scanning Probe / Q000295:instrumentation* / Q000379:methods*; D008962:Models, Theoretical; D009474:Neurons / Q000648:ultrastructure; D051381:Rats