title: Acute toxicity, cytotoxicity, genotoxicity and antigenotoxic effects of a cellulosic exopolysaccharide obtained from sugarcane molasses.
abstract: The acute toxicity, cytotoxicity, genotoxicity and antigenotoxic effects of BC were studied. Cytotoxicity of BC was evaluated in cultured C3A hepatoma cells (HepG2/C3A) using a lactate dehydrogenase (LDH) activity assay. Acute toxicity was tested in adults Wistar rats treated with a single dose of BC. The genotoxicity of BC was evaluated in vivo by the micronucleus assay. BC (0.33-170 μg/mL) added to C3A cell culture medium caused no elevation in LDH release over the background level recorded in untreated cell wells. The treatment with the BC in a single oral dose (2000 mg/kg body weight) caused no deaths or signs of toxicity. BC attenuated CP-induced and inhibition the incidence of MNPCE (female: 46.94%; male: 22.7%) and increased the ratio of PCE/NCE (female: 46.10%; male: 35.25%). There was no alteration in the LDH release in the wells where C3A cells were treated with increasing concentrations of BC compared to the wells where the cells received the cell culture medium only (background of approximately 20% cell death), indicated that in the dose range tested BC was not cytotoxic. BC was not cytotoxic, genotoxic or acutely toxic. BC attenuated CP-induced genotoxic and myelotoxic effects.
authors.affiliation: Center for Experimental Surgery, Department of Surgery, Center for Health Sciences, Federal University of Pernambuco, UFPE, Pernambuco, Brazil. Electronic address: fcmorone@gmail.com.
authors.forename: Flávia Cristina Morone
authors.initials: FCM
authors.lastname: Pinto
authors.affiliation: Laboratory of Environmental Toxicology, National School of Public Health, Oswaldo Cruz Foundation, FIOCRUZ, Rio de Janeiro, Brazil.
authors.forename: Ana Cecília A X
authors.initials: ACAX
authors.lastname: De-Oliveira
authors.affiliation: Laboratory of Environmental Toxicology, National School of Public Health, Oswaldo Cruz Foundation, FIOCRUZ, Rio de Janeiro, Brazil.
authors.forename: Rosangela R
authors.initials: RR
authors.lastname: De-Carvalho
authors.affiliation: Laboratory of Environmental Toxicology, National School of Public Health, Oswaldo Cruz Foundation, FIOCRUZ, Rio de Janeiro, Brazil.
authors.forename: Maria Regina
authors.initials: MR
authors.lastname: Gomes-Carneiro
authors.affiliation: Laboratory of Environmental Toxicology, National School of Public Health, Oswaldo Cruz Foundation, FIOCRUZ, Rio de Janeiro, Brazil.
authors.forename: Deise R
authors.initials: DR
authors.lastname: Coelho
authors.affiliation: Center for Experimental Surgery, Department of Surgery, Center for Health Sciences, Federal University of Pernambuco, UFPE, Pernambuco, Brazil.
authors.forename: Salvador Vilar C
authors.initials: SVC
authors.lastname: Lima
authors.affiliation: Laboratory of Environmental Toxicology, National School of Public Health, Oswaldo Cruz Foundation, FIOCRUZ, Rio de Janeiro, Brazil.
authors.forename: Francisco José R
authors.initials: FJR
authors.lastname: Paumgartten
authors.affiliation: Center for Experimental Surgery, Department of Surgery, Center for Health Sciences, Federal University of Pernambuco, UFPE, Pernambuco, Brazil.
authors.forename: José Lamartine A
authors.initials: JLA
authors.lastname: Aguiar
mesh_terms: D000818:Animals; D016587:Antimutagenic Agents / Q000737:chemistry; D002470:Cell Survival; D002482:Cellulose / Q000031:analogs & derivatives*; D004249:DNA Damage; D005260:Female; D056945:Hep G2 Cells; D006801:Humans; D008297:Male; D051379:Mice; D008965:Molasses / Q000382:microbiology*; D009153:Mutagens / Q000737:chemistry / Q000633:toxicity; D011135:Polysaccharides, Bacterial / Q000737:chemistry / Q000494:pharmacology / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D031786:Saccharum / Q000737:chemistry
chemical_list: D016587:Antimutagenic Agents; D009153:Mutagens; D011135:Polysaccharides, Bacterial; D002482:Cellulose
keywords: Antigenotoxicity; Bacterial Cellulose; Biomaterial; Cytotoxicity; Exopolysaccharide; Genotoxicity