title: Protective effect of soluble eggshell membrane protein hydrolysate on cardiac ischemia/reperfusion injury.
abstract: BACKGROUND
  Soluble eggshell membrane protein (SEP) has been proved to hold the antioxidant activity. The functional role of SEP on cardioprotection was investigated in vivo and in vitro.
  
  
  METHODS
  Rats and cardiomyocytes were pretreated with SP2, a hydrolysate attained from SEP, and then subjected to ischemia/reperfusion (I/R) or hypoxia/reoxygenation (H/R) and hydrogen peroxide, respectively. The measurement of myocardial infarct size, cell apoptosis assay, cell viability assay, and caspase activity assay were performed on rats and cardiomyocytes.
  
  
  RESULTS
  The results showed that the treatment of SP2 induced the resistance to I/R or H/R injury on rats and cardiomyocytes as indicated by decreased infarct size and decreased cellular apoptosis. The cardioprotective roles of SP2 were partly resulted from the downregulated expression and activity of caspase-3 in which the effect was similar to the caspase inhibitor, z-VAD-fmk, and could be rescued by caspase activator, PAC-1.
  
  
  CONCLUSIONS
  This investigation has demonstrated that SP2 attenuated the damage of I/R and H/R on rats and cardiomyocytes by the caspase-dependent pathway. This cardioprotective effect of SP2 suggested a novel therapeutic agent of SEP for ischemic-related heart diseases.
authors.affiliation: National Engineering Laboratory of Rice and By-product Deep Processing, Central South University of Forestry and Technology, Changsha, People's Republic of China.|College of Food Science & Engineering, Central South University of Forestry & Technology, Changsha, People's Republic of China.|Department of Histology and Embryology, School of Basic Medical Sciences, Central South University, Changsha, People's Republic of China.
authors.forename: Tao
authors.initials: T
authors.lastname: Yang
authors.affiliation: National Engineering Laboratory of Rice and By-product Deep Processing, Central South University of Forestry and Technology, Changsha, People's Republic of China.|College of Food Science & Engineering, Central South University of Forestry & Technology, Changsha, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: National R&D Center for Egg Processing, Huazhong Agricultural University, Wuhan, People's Republic of China.
authors.forename: Meihu
authors.initials: M
authors.lastname: Ma
authors.affiliation: National Engineering Laboratory of Rice and By-product Deep Processing, Central South University of Forestry and Technology, Changsha, People's Republic of China.|College of Food Science & Engineering, Central South University of Forestry & Technology, Changsha, People's Republic of China.
authors.forename: Qinlu
authors.initials: Q
authors.lastname: Lin
authors.affiliation: National Engineering Laboratory of Rice and By-product Deep Processing, Central South University of Forestry and Technology, Changsha, People's Republic of China.|College of Food Science & Engineering, Central South University of Forestry & Technology, Changsha, People's Republic of China.
authors.forename: Shuguo
authors.initials: S
authors.lastname: Sun
authors.affiliation: Department of Histology and Embryology, School of Basic Medical Sciences, Central South University, Changsha, People's Republic of China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Department of Histology and Embryology, School of Basic Medical Sciences, Central South University, Changsha, People's Republic of China.
authors.forename: Xi
authors.initials: X
authors.lastname: Feng
authors.affiliation: Department of Histology and Embryology, School of Basic Medical Sciences, Central South University, Changsha, People's Republic of China; liujunwen@csu.edu.cn.
authors.forename: Junwen
authors.initials: J
authors.lastname: Liu
keywords: cardiomyocytes; cardioprotection; caspase; ischemia-reperfusion injury; soluble eggshell membrane protein