title: Biocatalytic Resolution of Rac-α-Ethyl-2-Oxo-Pyrrolidineacetic Acid Methyl Ester by Immobilized Recombinant Bacillus cereus Esterase.
abstract: A new esterase-producing strain (Bacillus cereus WZZ001) which exhibiting high hydrolytic activity and excellent enantioselectivity on rac-α-ethyl-2-oxo-pyrrolidineacetic acid methyl ester (R, S-1) has been isolated from soil sample by our laboratory. In this study, the stereoselective hydrolysis of (R, S-1) was performed using the recombinant Bacillus cereus esterase which expressed in Escherichia coli BL21 (DE3). Under the optimized conditions of pH 8.0, 35 °C, and concentration of substrate 400 mM, a successful enzymatic resolution was achieved with an e.e. s of 99.5 % and conversion of 49 %. Immobilization considerably increased the reusability of the recombinant esterase; the immobilized enzyme showed excellent reusability during 6 cycles of repeated 2 h reactions at 35 °C. Thereby, it makes the recombinant B. cereus esterase a usable biocatalyst for industrial application.
authors.affiliation: College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou City, Zhejiang Province, 310014, People's Republic of China.
authors.forename: Jian-Yong
authors.initials: JY
authors.lastname: Zheng
authors.affiliation: College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou City, Zhejiang Province, 310014, People's Republic of China.
authors.forename: Yin-Yan
authors.initials: YY
authors.lastname: Liu
authors.affiliation: College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou City, Zhejiang Province, 310014, People's Republic of China.
authors.forename: Wei-Feng
authors.initials: WF
authors.lastname: Luo
authors.affiliation: College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou City, Zhejiang Province, 310014, People's Republic of China.
authors.forename: Ren-Chao
authors.initials: RC
authors.lastname: Zheng
authors.affiliation: College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou City, Zhejiang Province, 310014, People's Republic of China.
authors.forename: Xiang-Xian
authors.initials: XX
authors.lastname: Ying
authors.affiliation: College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou City, Zhejiang Province, 310014, People's Republic of China. hzwangzhao@163.com.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Wang
mesh_terms: D001409:Bacillus cereus / Q000737:chemistry / Q000201:enzymology*; D055162:Biocatalysis; D001709:Biotechnology; D004950:Esterases / Q000737:chemistry*; D011392:Proline / Q000031:analogs & derivatives / Q000737:chemistry; D011994:Recombinant Proteins / Q000737:chemistry*
chemical_list: D011994:Recombinant Proteins; D011392:Proline; C010983:homoproline; D004950:Esterases
keywords: Bacillus cereus; Enantioselective hydrolysis; Esterase; Recombinant; α-ethyl-2-oxo-pyrrolidineacetic acid methyl ester