title: Expressions of Thyroid Transcription Factor-1, Napsin A, p40, p63, CK5/6 and Desmocollin-3 in Non-Small Cell Lung Cancer, as Revealed by Imprint Cytology Using a Malinol-Based Cell-Transfer Technique.
abstract: BACKGROUND
  The introduction of new therapies has made it important to differentiate between adenocarcinoma and squamous cell carcinoma. To allow the use of various immunocytochemical stains on limited materials, we tried transferring cells from a given smear to multiple slides. Using touch-preparation samples of 215 surgically resected non-small cell lung carcinomas of confirmed histologic classification (adenocarcinoma,n = 101; squamous cell carcinoma,n = 114), we performed immunocytochemistry for thyroid transcription factor-1, napsin A, p40, p63, CK5/6 and desmocollin-3, and compared cytologic staining results with the corresponding resection.
  
  
  METHODS
  We examined: (a) the expressions of the above 6 antibodies on cells transferred from touch imprints of resected specimens, the extent of staining being considered positive if more than 5% of the area was stained, and (b) the sensitivity, specificity, positive predictive value and negative predictive value for each antibody.
  
  
  RESULTS
  The histologic corresponding rate with Papanicolaou staining was only 73%. Regarding the differentiation of adenocarcinoma from squamous cell carcinoma, the sensitivity and specificity for napsin A in adenocarcinoma were 80 and 97%, respectively, while those for p40 in squamous cell carcinoma were 84 and 98%, respectively.
  
  
  CONCLUSIONS
  The immunocytochemical expressions of napsin A and p40 in imprint cytology seem to be of great utility for the accurate histological differentiation of lung cancers.
authors.affiliation: Department of Pathology and Laboratory Medicine, National Defense Medical College, Saitama, Japan.
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Kawai
authors.forename: Susumu
authors.initials: S
authors.lastname: Tominaga
authors.forename: Sadayuki
authors.initials: S
authors.lastname: Hiroi
authors.forename: Koji
authors.initials: K
authors.lastname: Kameda
authors.forename: Sho
authors.initials: S
authors.lastname: Ogata
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nakashima
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Ozeki
authors.forename: Kuniaki
authors.initials: K
authors.lastname: Nakanishi
mesh_terms: D000230:Adenocarcinoma / Q000737:chemistry* / Q000473:pathology / Q000601:surgery; D000077192:Adenocarcinoma of Lung; D016282:Aspartic Acid Endopeptidases / Q000032:analysis*; D014408:Biomarkers, Tumor / Q000032:analysis*; D002289:Carcinoma, Non-Small-Cell Lung / Q000737:chemistry* / Q000473:pathology / Q000601:surgery; D002294:Carcinoma, Squamous Cell / Q000737:chemistry* / Q000473:pathology / Q000601:surgery; D002454:Cell Differentiation; D051187:Desmocollins / Q000032:analysis; D003937:Diagnosis, Differential; D006801:Humans; D007150:Immunohistochemistry; D053555:Keratin-5 / Q000032:analysis; D053553:Keratin-6 / Q000032:analysis; D008175:Lung Neoplasms / Q000737:chemistry* / Q000473:pathology / Q000601:surgery; D009687:Nuclear Proteins / Q000032:analysis; D065006:Papanicolaou Test; D011237:Predictive Value of Tests; D013048:Specimen Handling / Q000379:methods*; D000074482:Thyroid Nuclear Factor 1; D014157:Transcription Factors / Q000032:analysis*; D025521:Tumor Suppressor Proteins / Q000032:analysis*
chemical_list: D014408:Biomarkers, Tumor; C490356:DSC3 protein, human; D051187:Desmocollins; C503940:KRT5 protein, human; D053555:Keratin-5; D053553:Keratin-6; C000614412:NKX2-1 protein, human; D009687:Nuclear Proteins; C115051:TP63 protein, human; D000074482:Thyroid Nuclear Factor 1; D014157:Transcription Factors; D025521:Tumor Suppressor Proteins; D016282:Aspartic Acid Endopeptidases; C498549:NAPSA protein, human