title: Rapid Alterations in Perirenal Adipose Tissue Transcriptomic Networks with Cessation of Voluntary Running.
abstract: In maturing rats, the growth of abdominal fat is attenuated by voluntary wheel running. After the cessation of running by wheel locking, a rapid increase in adipose tissue growth to a size that is similar to rats that have never run (i.e. catch-up growth) has been previously reported by our lab. In contrast, diet-induced increases in adiposity have a slower onset with relatively delayed transcriptomic responses. The purpose of the present study was to identify molecular pathways associated with the rapid increase in adipose tissue after ending 6 wks of voluntary running at the time of puberty. Age-matched, male Wistar rats were given access to running wheels from 4 to 10 weeks of age. From the 10th to 11th week of age, one group of rats had continued wheel access, while the other group had one week of wheel locking. Perirenal adipose tissue was extracted, RNA sequencing was performed, and bioinformatics analyses were executed using Ingenuity Pathway Analysis (IPA). IPA was chosen to assist in the understanding of complex 'omics data by integrating data into networks and pathways. Wheel locked rats gained significantly more fat mass and significantly increased body fat percentage between weeks 10-11 despite having decreased food intake, as compared to rats with continued wheel access. IPA identified 646 known transcripts differentially expressed (p < 0.05) between continued wheel access and wheel locking. In wheel locked rats, IPA revealed enrichment of transcripts for the following functions: extracellular matrix, macrophage infiltration, immunity, and pro-inflammatory. These findings suggest that increases in visceral adipose tissue that accompanies the cessation of pubertal physical activity are associated with the alteration of multiple pathways, some of which may potentiate the development of pubertal obesity and obesity-associated systemic low-grade inflammation that occurs later in life.
authors.affiliation: Department of Biomedical Science, College of Veterinary Medicine, University of Missouri, Columbia, Missouri, United States of America.
authors.forename: Gregory N
authors.initials: GN
authors.lastname: Ruegsegger
authors.affiliation: Department of Biomedical Science, College of Veterinary Medicine, University of Missouri, Columbia, Missouri, United States of America.
authors.forename: Joseph M
authors.initials: JM
authors.lastname: Company
authors.affiliation: Department of Biomedical Science, College of Veterinary Medicine, University of Missouri, Columbia, Missouri, United States of America.
authors.forename: Ryan G
authors.initials: RG
authors.lastname: Toedebusch
authors.affiliation: Geriatrics, Research, Education and Clinical Center, VA of Greater Los Angeles Healthcare System, Los Angeles, California, United States of America.
authors.forename: Christian K
authors.initials: CK
authors.lastname: Roberts
authors.affiliation: Department of Biomedical Science, College of Veterinary Medicine, University of Missouri, Columbia, Missouri, United States of America.
authors.forename: Michael D
authors.initials: MD
authors.lastname: Roberts
authors.affiliation: Department of Biomedical Science, College of Veterinary Medicine, University of Missouri, Columbia, Missouri, United States of America.|Department of Medical Pharmacology and Physiology, University of Missouri, Columbia, Missouri, United States of America.|Department of Nutrition and Exercise Physiology, University of Missouri, Columbia, Missouri, United States of America.|Dalton Cardiovascular Research Center, University of Missouri, Columbia, Missouri, United States of America.
authors.forename: Frank W
authors.initials: FW
authors.lastname: Booth
mesh_terms: D015502:Absorptiometry, Photon; D017667:Adipocytes / Q000648:ultrastructure; D050156:Adipogenesis / Q000502:physiology*; D000818:Animals; D015153:Blotting, Western; D002452:Cell Count; D004435:Eating / Q000502:physiology; D004734:Energy Metabolism / Q000502:physiology; D020869:Gene Expression Profiling; D050152:Intra-Abdominal Fat / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D008297:Male; D005082:Physical Exertion / Q000502:physiology*; D012313:RNA / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar
chemical_list: D012313:RNA