title: Profiling of Sox4-dependent transcriptome in skin links tumour suppression and adult stem cell activation.
abstract: Adult stem cells (ASCs) reside in specific niches in a quiescent state in adult mammals. Upon specific cues they become activated and respond by self-renewing and differentiating into newly generated specialised cells that ensure appropriate tissue fitness. ASC quiescence also serves as a tumour suppression mechanism by hampering cellular transformation and expansion (White AC et al., 2014). Some genes restricted to early embryonic development and adult stem cell niches are often potent modulators of stem cell quiescence, and derailed expression of these is commonly associated to cancer (Vervoort SJ et al., 2013). Among them, it has been shown that recommissioned Sox4 expression facilitates proliferation, survival and migration of malignant cells. By generating a conditional Knockout mouse model in stratified epithelia (Sox4 (cKO) mice), we demonstrated a delayed plucking-induced Anagen in the absence of Sox4. Skin global transcriptome analysis revealed a prominent defect in the induction of transcriptional networks that control hair follicle stem cell (HFSC) activation such as those regulated by Wnt/Ctnnb1, Shh, Myc or Sox9, cell cycle and DNA damage response-associated pathways. Besides, Sox4 (cKO) mice are resistant to skin carcinogenesis, thus linking Sox4 to both normal and pathological HFSC activation (Foronda M et al., 2014). Here we provide additional details on the analysis of Sox4-regulated transcriptome in Telogen and Anagen skin. The raw and processed microarray data is deposited in GEO under GSE58155.
authors.affiliation: Telomeres and Telomerase Group, Molecular Oncology Program, Spanish National Cancer Research Centre (CNIO), C/ Melchor Fernández Almagro, 3, E-28029, Madrid, Spain.
authors.forename: Miguel
authors.initials: M
authors.lastname: Foronda
authors.affiliation: Tumour Suppression Group, Molecular Oncology Program, Spanish National Cancer Research Centre (CNIO), C/ Melchor Fernández Almagro, 3, E-28029, Madrid, Spain.
authors.forename: Lucia
authors.initials: L
authors.lastname: Morgado-Palacin
authors.affiliation: Bioinformatics Unit, Structural Biology and Biocomputing Program, Spanish National Cancer Research Centre (CNIO), C/ Melchor Fernández Almagro, 3, E-28029, Madrid, Spain.
authors.forename: Gonzalo
authors.initials: G
authors.lastname: Gómez-López
authors.affiliation: Genomics Unit, Biotechnology Program, Spanish National Cancer Research Centre (CNIO), C/Melchor Fernández Almagro, 3, E-28029 Madrid, Spain.
authors.forename: Orlando
authors.initials: O
authors.lastname: Domínguez
authors.affiliation: Bioinformatics Unit, Structural Biology and Biocomputing Program, Spanish National Cancer Research Centre (CNIO), C/ Melchor Fernández Almagro, 3, E-28029, Madrid, Spain.
authors.forename: David G
authors.initials: DG
authors.lastname: Pisano
authors.affiliation: Telomeres and Telomerase Group, Molecular Oncology Program, Spanish National Cancer Research Centre (CNIO), C/ Melchor Fernández Almagro, 3, E-28029, Madrid, Spain.
authors.forename: Maria A
authors.initials: MA
authors.lastname: Blasco
keywords: Adult stem cells; Cancer; Microarray; Skin; Sox4