title: Understanding the link between single cell and population scale responses of Escherichia coli in differing ligand gradients.
abstract: We formulate an agent-based population model of Escherichia coli cells which incorporates a description of the chemotaxis signalling cascade at the single cell scale. The model is used to gain insight into the link between the signalling cascade dynamics and the overall population response to differing chemoattractant gradients. Firstly, we consider how the observed variation in total (phosphorylated and unphosphorylated) signalling protein concentration affects the ability of cells to accumulate in differing chemoattractant gradients. Results reveal that a variation in total cell protein concentration between cells may be a mechanism for the survival of cell colonies across a wide range of differing environments. We then study the response of cells in the presence of two different chemoattractants. In doing so we demonstrate that the population scale response depends not on the absolute concentration of each chemoattractant but on the sensitivity of the chemoreceptors to their respective concentrations. Our results show the clear link between single cell features and the overall environment in which cells reside.
authors.affiliation: Department of Mathematics & Statistics, University of Reading, Whiteknights, PO Box 220, Reading RG6 6AX, UK.
authors.forename: Matthew P
authors.initials: MP
authors.lastname: Edgington
authors.affiliation: Department of Mathematics & Statistics, University of Reading, Whiteknights, PO Box 220, Reading RG6 6AX, UK ; Institute for Cardiovascular and Metabolic Research, University of Reading, Whiteknights, PO Box 218, Reading RG6 6AA, UK.
authors.forename: Marcus J
authors.initials: MJ
authors.lastname: Tindall
keywords: Agent-based modelling; Bacteria; Chemotaxis; Escherichia coli; Multi-scale; Signalling cascade