title: Updates on the Production of Therapeutic Antibodies Using Human Hybridoma Technique.
abstract: Therapeutic antibodies are implicated into the very promising and fast growing area of pharmaceutics. Human hybridoma technology, allowing generation of natural human antibodies in a native form, seems to be the most direct way that require no additional modifications for production of therapeutic antibodies. However, technical difficulties in human hybridoma creation discovered in the 80s of the last century have switched the mainstream therapeutic antibody development into new directions like display and transgenic mice techniques. These approaches have provided remarkable achievements in antibody engineering within last 15 years, but also revealed other limitations. Thus, it is time to turn back to forgotten human hybridoma technology. In this review, we describe new advances in all components of human hybridoma technology and discuss challenges in generating novel therapeutic mABs based on hybridoma technologies.
authors.forename: Xenia A
authors.initials: XA
authors.lastname: Glukhova
authors.forename: Olga V
authors.initials: OV
authors.lastname: Prusakova
authors.forename: Julia A
authors.initials: JA
authors.lastname: Trizna
authors.forename: Michael M
authors.initials: MM
authors.lastname: Zaripov
authors.forename: Gaida V
authors.initials: GV
authors.lastname: Afanas'eva
authors.forename: Anatoly S
authors.initials: AS
authors.lastname: Glukhov
authors.forename: Rimma A
authors.initials: RA
authors.lastname: Poltavtseva
authors.forename: Alexei A
authors.initials: AA
authors.lastname: Ivanov
authors.forename: Marco
authors.initials: M
authors.lastname: Avila-Rodriguez
authors.forename: George E
authors.initials: GE
authors.lastname: Barreto
authors.forename: Gjumrakch
authors.initials: G
authors.lastname: Aliev
authors.affiliation: Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Institutskaya st. 3, Pushchino, 142290 Moscow Region, Russia. ipbeletsky@gmail.com.
authors.forename: Igor P
authors.initials: IP
authors.lastname: Beletsky
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000627:therapeutic use; D020811:Biomedical Technology / Q000379:methods*; D015195:Drug Design; D006801:Humans; D006825:Hybridomas / Q000276:immunology*; D051379:Mice; D008822:Mice, Transgenic
chemical_list: D000911:Antibodies, Monoclonal