title: TLE4 promotes colorectal cancer progression through activation of JNK/c-Jun signaling pathway.
abstract: The Groucho transcriptional co-repressor TLE4 protein has been shown to be a tumor suppressor in a subset of acute myeloid leukemia. However, little is known about its role in development and progression of solid tumor. In this study, we found that the expression of TLE4 in colorectal cancer (CRC) tissues was significantly higher than that in their matched adjacent intestine epithelial tissues. In addition, high expression of TLE4 was significantly correlated with advanced Dukes stage, lymph node metastasis and poor prognosis of CRC. Moreover, enforced expression of TLE4 in CRC cell lines significantly enhanced proliferation, invasion and tumor growth. On the contrary, knock down of TLE4 repressed cell proliferation, invasion and tumor growth. Furthermore, our study exhibited that the TLE4 promoted cell proliferation and invasion partially via activation of JNK-c-Jun pathway and subsequently increased cyclinD1 and decreased P27Kip1 expression. In conclusion, these results suggested that TLE4, a potential prognostic biomarker for CRC, plays an important role in the development and progression of human CRC.
authors.affiliation: Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China.|Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, Guangdong, China.|State Key Laboratory of Oncology in Southern China, Department of Experimental, Guangzhou, Guangdong, China.
authors.forename: Shu-Yang
authors.initials: SY
authors.lastname: Wang
authors.affiliation: Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China.|Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, Guangdong, China.|State Key Laboratory of Oncology in Southern China, Department of Experimental, Guangzhou, Guangdong, China.|Department of Pathology, The Fifth Affiliated Hospital of Southern Medical University, Guangzhou, Guangdong, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Gao
authors.affiliation: Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China.|Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, Guangdong, China.|State Key Laboratory of Oncology in Southern China, Department of Experimental, Guangzhou, Guangdong, China.
authors.forename: Dan-Ling
authors.initials: DL
authors.lastname: Deng
authors.affiliation: Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China.|Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, Guangdong, China.|State Key Laboratory of Oncology in Southern China, Department of Experimental, Guangzhou, Guangdong, China.
authors.forename: Juan-Juan
authors.initials: JJ
authors.lastname: Cai
authors.affiliation: Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China.|Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, Guangdong, China.|State Key Laboratory of Oncology in Southern China, Department of Experimental, Guangzhou, Guangdong, China.
authors.forename: Zhi-Yuan
authors.initials: ZY
authors.lastname: Xiao
authors.affiliation: Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China.|Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, Guangdong, China.|State Key Laboratory of Oncology in Southern China, Department of Experimental, Guangzhou, Guangdong, China.
authors.forename: Liu-Qing
authors.initials: LQ
authors.lastname: He
authors.affiliation: Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China.|Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, Guangdong, China.|State Key Laboratory of Oncology in Southern China, Department of Experimental, Guangzhou, Guangdong, China.
authors.forename: Hong-Li
authors.initials: HL
authors.lastname: Jiao
authors.affiliation: Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China.|Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, Guangdong, China.|State Key Laboratory of Oncology in Southern China, Department of Experimental, Guangzhou, Guangdong, China.
authors.forename: Ya-Ping
authors.initials: YP
authors.lastname: Ye
authors.affiliation: Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China.|Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, Guangdong, China.|State Key Laboratory of Oncology in Southern China, Department of Experimental, Guangzhou, Guangdong, China.
authors.forename: Run-Wei
authors.initials: RW
authors.lastname: Yang
authors.affiliation: Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China.|Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, Guangdong, China.|State Key Laboratory of Oncology in Southern China, Department of Experimental, Guangzhou, Guangdong, China.
authors.forename: Ting-Ting
authors.initials: TT
authors.lastname: Li
authors.affiliation: Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China.|Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, Guangdong, China.|State Key Laboratory of Oncology in Southern China, Department of Experimental, Guangzhou, Guangdong, China.
authors.forename: Li
authors.initials: L
authors.lastname: Liang
authors.affiliation: Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China.|Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, Guangdong, China.|State Key Laboratory of Oncology in Southern China, Department of Experimental, Guangzhou, Guangdong, China.
authors.forename: Wen-Ting
authors.initials: WT
authors.lastname: Liao
authors.affiliation: Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China.|Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, Guangdong, China.|State Key Laboratory of Oncology in Southern China, Department of Experimental, Guangzhou, Guangdong, China.
authors.forename: Yan-Qing
authors.initials: YQ
authors.lastname: Ding
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000378:metabolism; D018938:Caco-2 Cells; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015179:Colorectal Neoplasms / Q000473:pathology*; D019938:Cyclin D1 / Q000096:biosynthesis; D050760:Cyclin-Dependent Kinase Inhibitor p27 / Q000096:biosynthesis; D018450:Disease Progression; D004789:Enzyme Activation; D045325:HCT116 Cells; D019073:HT29 Cells; D006801:Humans; D048031:JNK Mitogen-Activated Protein Kinases / Q000378:metabolism*; D008207:Lymphatic Metastasis / Q000473:pathology; D020935:MAP Kinase Signaling System / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009361:Neoplasm Invasiveness / Q000473:pathology; D009368:Neoplasm Transplantation; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D011379:Prognosis; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D014183:Transplantation, Heterologous; D025521:Tumor Suppressor Proteins / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; C530104:CCND1 protein, human; C436291:CDKN1B protein, human; D009687:Nuclear Proteins; D034741:RNA, Small Interfering; D012097:Repressor Proteins; C465817:TLE4 protein, human; D025521:Tumor Suppressor Proteins; D019938:Cyclin D1; D050760:Cyclin-Dependent Kinase Inhibitor p27; D048031:JNK Mitogen-Activated Protein Kinases
keywords: JNK; TLE4; c-Jun; colorectal cancer; proliferation