title: Radical scavenging, antioxidant, and cytotoxic activities of the methanolic extracts from different organs of Ternstroemia pringlei.
abstract: Ternstroemia pringlei (Rose) Standl. (Theaceae) is widely used in Mexican traditional medicine to treat a diverse array of illnesses including rheumatoid pains, and is listed as one of the most consumed medicinal plants in the country. We selected T. pringlei given the strong relationship between oxidative stress and arthritis pathology, and investigated antioxidant potential of leaf, petal, fruit and seed methanolic extracts. Our method included assessing the in vitro free radical scavenger activity using the 2,2´-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) test, as well as the in vivo antioxidant action in the H2O2 protection model with Saccharomyces cerevisiae. Leaves and fruits afforded the most active extract in the ABTS assay, with antiradical activity of IC50=33.91 and 38.09μg/mL, respectively; while fruit extracts at 250μg/mL proved the most protective action against H2O2 oxidative stress. All extracts were non-cytotoxic against HF-6 (colon), PC-3 (prostate), MCF-7 (breast), SiHa (cervical) cancer cell lines and also toward the HFS-30 fibroblast normal skin cell line (IC50&>20μg/mL). Leaf methanolic extracts afforded ternstroside B, a known phenylethanoid glycoside with a strong free radical scavenging action. The presence of this kind of metabolites opens new research perspectives for the plant.
authors.affiliation: Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Morelos, Mexico.
authors.forename: Nahim
authors.initials: N
authors.lastname: Salgado
authors.affiliation: Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Morelos, Mexico.
authors.forename: Pablo Noé-Núñez
authors.initials: PN
authors.lastname: Aragón
authors.affiliation: Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Morelos, Mexico.
authors.forename: Macdiel
authors.initials: M
authors.lastname: Acevedo
authors.affiliation: Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Morelos, Mexico.
authors.forename: Lucia
authors.initials: L
authors.lastname: Corona
authors.affiliation: Facultad de Farmacia, Universidad Autónoma del Estado de Morelos, Morelos, Mexico.
authors.forename: Verónica
authors.initials: V
authors.lastname: Rodríguez
authors.affiliation: Faculté de Pharmacie, University de Picardie Jules Verne, Amiens, France.
authors.forename: François
authors.initials: F
authors.lastname: Mesnard
authors.affiliation: Faculté de Pharmacie, University de Picardie Jules Verne, Amiens, France.
authors.forename: Marc-André
authors.initials: MA
authors.lastname: Fliniaux
authors.affiliation: Faculté de Pharmacie, University de Picardie Jules Verne, Amiens, France.
authors.forename: Roland
authors.initials: R
authors.lastname: Molinié
authors.affiliation: Plateforme ICAP, UPJV, Centre Universitaire de Recherche en Santé (CURS), Amiens, France.
authors.forename: Paulo
authors.initials: P
authors.lastname: Marcelo
authors.affiliation: Faculté de Pharmacie, University de Picardie Jules Verne, Amiens, France.
authors.forename: María Luisa
authors.initials: ML
authors.lastname: Villarreal
authors.affiliation: Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Morelos, Mexico.
authors.forename: Alexandre Toshirrico Cardoso
authors.initials: AT
authors.lastname: Taketa