title: Enhancing the lipid productivity of yeasts with trace concentrations of iron nanoparticles.
abstract: Oxidative stress induced by zero-valent iron nanoparticles (nZVIs) was used to improve lipid accumulation in various oleaginous and non-oleginous yeasts-Candida sp., Kluyveromyces polysporus, Rhodotorula glutinis, Saccharomyces cerevisiae, Torulospora delbrueckii, Trichosporon cutaneum, and Yarrowia lipolytica. The highest lipid yields occurred at 9-13 mg/L nZVIs. Gas chromatography-mass spectrometry was used for the quantitative and qualitative analysis of the fatty acids. It showed an increasing abundance of polyunsaturated fatty acids, especially essential linoleic acid, in the presence of nZVIs. Our results suggest that nZVIs can be used to improve not only lipid production by oleaginous microorganisms but also the nutritional value of biosynthesized unsaturated fatty acids.
authors.affiliation: Department of Biotechnology, University of Chemical Technology Prague, Technická 5, 166 28, Prague, Czech Republic.
authors.forename: Karolína
authors.initials: K
authors.lastname: Pádrová
authors.affiliation: Department of Biotechnology, University of Chemical Technology Prague, Technická 5, 166 28, Prague, Czech Republic.
authors.forename: Alena
authors.initials: A
authors.lastname: Čejková
authors.affiliation: Institute of Microbiology, CAS, Vídeňská 1083, 142 20, Prague, Czech Republic.
authors.forename: Tomáš
authors.initials: T
authors.lastname: Cajthaml
authors.affiliation: Department of Biotechnology, University of Chemical Technology Prague, Technická 5, 166 28, Prague, Czech Republic.
authors.forename: Irena
authors.initials: I
authors.lastname: Kolouchová
authors.affiliation: Laboratory of Cell Cycles of Algae, Centre Algatech, Institute of Microbiology, CAS, Opatovický mlýn 237, 379 81, Třeboň, Czech Republic.
authors.forename: Milada
authors.initials: M
authors.lastname: Vítová
authors.affiliation: Institute of Microbiology, CAS, Vídeňská 1083, 142 20, Prague, Czech Republic.
authors.forename: Karel
authors.initials: K
authors.lastname: Sigler
authors.affiliation: Institute of Microbiology, CAS, Vídeňská 1083, 142 20, Prague, Czech Republic. rezanka@biomed.cas.cz.
authors.forename: Tomáš
authors.initials: T
authors.lastname: Řezanka
mesh_terms: D003600:Cytosol / Q000737:chemistry; D005227:Fatty Acids / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry; D007501:Iron / Q000378:metabolism*; D050356:Lipid Metabolism; D053758:Nanoparticles / Q000378:metabolism*; D018384:Oxidative Stress; D015003:Yeasts / Q000187:drug effects* / Q000378:metabolism
chemical_list: D005227:Fatty Acids; D007501:Iron