title: Resistome and pathogenomics of multidrug resistant (MDR) Pseudomonas aeruginosa VRFPA03, VRFPA05 recovered from alkaline chemical keratitis and post-operative endophthalmitis patient.
abstract: Eye infections due to Pseudomonas aeruginosa is an important cause of ocular morbidity. We presents the whole genomic comparative analysis of two P. aeruginosa VRFPA03 and VRFPA05 isolated from alkaline chemical injury mediated keratitis and post-cataract surgery endophthalmitis patients, respectively. The blaDIM-1 gene in VRFPA03 and the blaGes-9 gene in VRFPA05 were identified and reported for the first time from an ocular isolate. The current study revealed novel integrons In1107 and In1108, comprised of multidrug-resistant genes. Ocular virulence factors mainly mediated by exoenzymes T, Y, and U and exotoxin A, elastase B, and phenazine-specific methyltransferase. Genomic analysis uncovered multiple known and unknown factors involved in P. aeruginosa mediated ocular infection, which may lead to drug discovery and diagnostic markers to improve human vision care.
authors.affiliation: Dept of Microbiology, L & T Microbiology Research Centre, Vision Research Foundation, Sankara Nethralaya, Chennai, Tamil Nadu 6000 06, India.
authors.forename: Nandagopal
authors.initials: N
authors.lastname: Murugan
authors.affiliation: Dept of Microbiology, L & T Microbiology Research Centre, Vision Research Foundation, Sankara Nethralaya, Chennai, Tamil Nadu 6000 06, India. Electronic address: drjm@snmail.org.
authors.forename: Jambulingam
authors.initials: J
authors.lastname: Malathi
authors.affiliation: Centre for Bioinformatics, Vision Research Foundation, Sankara Nethralaya, Chennai, Tamil Nadu 6000 06, India.
authors.forename: Vetrivel
authors.initials: V
authors.lastname: Umashankar
authors.affiliation: Dept of Microbiology, L & T Microbiology Research Centre, Vision Research Foundation, Sankara Nethralaya, Chennai, Tamil Nadu 6000 06, India.
authors.forename: Hajib NarahariRao
authors.initials: HN
authors.lastname: Madhavan
mesh_terms: D002387:Cataract Extraction; D024901:Drug Resistance, Multiple, Bacterial; D009877:Endophthalmitis / Q000382:microbiology* / Q000601:surgery; D016680:Genome, Bacterial; D006801:Humans; D007634:Keratitis / Q000382:microbiology*; D011552:Pseudomonas Infections / Q000382:microbiology / Q000601:surgery; D011550:Pseudomonas aeruginosa / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D017422:Sequence Analysis, DNA; D037521:Virulence Factors / Q000235:genetics*
chemical_list: D037521:Virulence Factors
keywords: Exotoxins; Genomics; Integrons; Multidrug efflux pump genes; Multilocus sequence typing; Pseudomonas aeruginosa; Virulence factors