title: Mechanism of regulation of tomato TRN1 gene expression in late infection with tomato leaf curl New Delhi virus (ToLCNDV).
abstract: Tomato leaf curl disease caused by geminiviruses is manifested by curling and puckering of leaves and thickening of veins, resembling developmental defects. This is probably due to the long-term altered regulation of expression of development related gene(s). Our results show that in the infected leaves the transcript level of TORNADO1 (SlTRN1), a gene important for cell expansion and vein formation, increased significantly. SlTRN1 is transcribed from two start sites. The preferential usage of one start site governs its expression in viral-stressed plants. To investigate the role of specific promoter elements in mediating differential expression of SlTRN1, we performed SlTRN1 promoter analysis. The promoter-regulatory sequences harbor multiple W-boxes. The SlWRKY16 transcription factor actively interacts with one of the W-boxes. WRKY proteins are commonly induced by salicylic acid (SA), and consequently SA treatment increased transcript level of SlWRKY16 and SlTRN1. Further mutational analyses confirmed the role of W-boxes in mediating SlTRN1 induction during ToLCNDV infection or SA treatment. We postulate that the activation of SA pathway during stress-response in tomato induces WRKY16, which in turn modulates transcription of SlTRN1 gene. This study unravels the mechanism of regulation of a developmental gene during stress-response, which may affect the severity of symptoms.
authors.affiliation: Division of Plant Biology, Bose Institute, P1/12CIT Scheme VII(M), Kolkata 700054, India.
authors.forename: Arunava
authors.initials: A
authors.lastname: Mandal
authors.affiliation: Division of Plant Biology, Bose Institute, P1/12CIT Scheme VII(M), Kolkata 700054, India.
authors.forename: Deepti
authors.initials: D
authors.lastname: Sarkar
authors.affiliation: Botany Department, University of Calcutta, 35 Ballygunge Circular Rd, Ballygunge, Kolkata 700019, India.
authors.forename: Surekha
authors.initials: S
authors.lastname: Kundu
authors.affiliation: Division of Plant Biology, Bose Institute, P1/12CIT Scheme VII(M), Kolkata 700054, India. Electronic address: pkundu@jcbose.ac.in.
authors.forename: Pallob
authors.initials: P
authors.lastname: Kundu
mesh_terms: D001483:Base Sequence; D053839:Begomovirus / Q000502:physiology*; D018506:Gene Expression Regulation, Plant; D018551:Solanum lycopersicum / Q000235:genetics* / Q000378:metabolism / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology; D018515:Plant Leaves / Q000378:metabolism / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D010940:Plant Proteins
keywords: Promoter analysis; Salicylic acid; Stress response; TRN1; Tomato leaf curl virus; WRKY16