title: Highly Sensitive Colorimetric Detection of Ochratoxin A by a Label-Free Aptamer and Gold Nanoparticles.
abstract: A label-free aptamer-based assay for the highly sensitive and specific detection of Ochratoxin A (OTA) was developed using a cationic polymer and gold nanoparticles (AuNPs). The OTA aptamer was used as a recognition element for the colorimetric detection of OTA based on the aggregation of AuNPs by the cationic polymer. By spectroscopic quantitative analysis, the colorimetric assay could detect OTA down to 0.009 ng/mL with high selectivity in the presence of other interfering toxins. This study offers a new alternative in visual detection methods that is rapid and sensitive for OTA detection.
authors.affiliation: Agriculture Environment, Beijing Research Center for Agricultural Standards and Testing, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China. luanyx@nercita.org.cn.|Risk Assessment Lab for Agro-products (Beijing), Ministry of Agriculture, Beijing 100097, China. luanyx@nercita.org.cn.
authors.forename: Yunxia
authors.initials: Y
authors.lastname: Luan
authors.affiliation: Agriculture Environment, Beijing Research Center for Agricultural Standards and Testing, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China. cjy_1666@126.com.|Risk Assessment Lab for Agro-products (Beijing), Ministry of Agriculture, Beijing 100097, China. cjy_1666@126.com.
authors.forename: Jiayi
authors.initials: J
authors.lastname: Chen
authors.affiliation: Agriculture Environment, Beijing Research Center for Agricultural Standards and Testing, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China. lic@nercita.org.cn.|Risk Assessment Lab for Agro-products (Beijing), Ministry of Agriculture, Beijing 100097, China. lic@nercita.org.cn.
authors.forename: Cheng
authors.initials: C
authors.lastname: Li
authors.affiliation: Grain Safety, Academy of State Administration of Grain, Beijing 100037, China. wsx@chinagrain.org.
authors.forename: Gang
authors.initials: G
authors.lastname: Xie
authors.affiliation: Agriculture Environment, Beijing Research Center for Agricultural Standards and Testing, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China. fhailong84@126.com.|Risk Assessment Lab for Agro-products (Beijing), Ministry of Agriculture, Beijing 100097, China. fhailong84@126.com.
authors.forename: Hailong
authors.initials: H
authors.lastname: Fu
authors.affiliation: Agriculture Environment, Beijing Research Center for Agricultural Standards and Testing, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China. mazh@nercita.org.cn.|Risk Assessment Lab for Agro-products (Beijing), Ministry of Agriculture, Beijing 100097, China. mazh@nercita.org.cn.
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Ma
authors.affiliation: Agriculture Environment, Beijing Research Center for Agricultural Standards and Testing, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China. luax@nercita.org.cn.|Risk Assessment Lab for Agro-products (Beijing), Ministry of Agriculture, Beijing 100097, China. luax@nercita.org.cn.
authors.forename: Anxiang
authors.initials: A
authors.lastname: Lu
mesh_terms: D000434:Alcoholic Beverages / Q000032:analysis; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D003124:Colorimetry; D005506:Food Contamination / Q000032:analysis; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D009793:Ochratoxins / Q000032:analysis* / Q000737:chemistry; D011095:Polyethylenes / Q000737:chemistry; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D012965:Sodium Chloride / Q000737:chemistry
chemical_list: D052157:Aptamers, Nucleotide; D009793:Ochratoxins; D011095:Polyethylenes; D000644:Quaternary Ammonium Compounds; C025589:ochratoxin A; C041004:poly-N,N-dimethyl-N,N-diallylammonium chloride; D012965:Sodium Chloride; D006046:Gold
keywords: Ochratoxin A (OTA); aptamer; cationic polymer; colorimetric assay; gold nanoparticles