title: Control of Synaptic Connectivity by a Network of Drosophila IgSF Cell Surface Proteins.
abstract: We have defined a network of interacting Drosophila cell surface proteins in which a 21-member IgSF subfamily, the Dprs, binds to a nine-member subfamily, the DIPs. The structural basis of the Dpr-DIP interaction code appears to be dictated by shape complementarity within the Dpr-DIP binding interface. Each of the six dpr and DIP genes examined here is expressed by a unique subset of larval and pupal neurons. In the neuromuscular system, interactions between Dpr11 and DIP-γ affect presynaptic terminal development, trophic factor responses, and neurotransmission. In the visual system, dpr11 is selectively expressed by R7 photoreceptors that use Rh4 opsin (yR7s). Their primary synaptic targets, Dm8 amacrine neurons, express DIP-γ. In dpr11 or DIP-γ mutants, yR7 terminals extend beyond their normal termination zones in layer M6 of the medulla. DIP-γ is also required for Dm8 survival or differentiation. Our findings suggest that Dpr-DIP interactions are important determinants of synaptic connectivity.
authors.affiliation: Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA.
authors.forename: Robert A
authors.initials: RA
authors.lastname: Carrillo
authors.affiliation: Dept. of Biochemistry and Molecular Biology, University of Chicago, Chicago, IL.|Howard Hughes Medical Institute, Depts. of Molecular and Cellular Physiology and Structural Biology, Stanford University School of Medicine, Stanford, CA.
authors.forename: Engin
authors.initials: E
authors.lastname: Özkan
authors.affiliation: Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA.
authors.forename: Kaushiki P
authors.initials: KP
authors.lastname: Menon
authors.affiliation: Howard Hughes Medical Institute, Program in Developmental Biology, Dept. of Molecular and Human Genetics, Jan and Dan Duncan Neurological Research Institute at TCH, Baylor College of Medicine, Houston, TX.
authors.forename: Sonal
authors.initials: S
authors.lastname: Nagarkar-Jaiswal
authors.affiliation: Howard Hughes Medical Institute, Program in Developmental Biology, Dept. of Molecular and Human Genetics, Jan and Dan Duncan Neurological Research Institute at TCH, Baylor College of Medicine, Houston, TX.
authors.forename: Pei-Tseng
authors.initials: PT
authors.lastname: Lee
authors.affiliation: Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA.|Howard Hughes Medical Institute, Depts. of Molecular and Cellular Physiology and Structural Biology, Stanford University School of Medicine, Stanford, CA.
authors.forename: Mili
authors.initials: M
authors.lastname: Jeon
authors.affiliation: Howard Hughes Medical Institute, Depts. of Molecular and Cellular Physiology and Structural Biology, Stanford University School of Medicine, Stanford, CA.
authors.forename: Michael E
authors.initials: ME
authors.lastname: Birnbaum
authors.affiliation: Howard Hughes Medical Institute, Program in Developmental Biology, Dept. of Molecular and Human Genetics, Jan and Dan Duncan Neurological Research Institute at TCH, Baylor College of Medicine, Houston, TX.
authors.forename: Hugo J
authors.initials: HJ
authors.lastname: Bellen
authors.affiliation: Howard Hughes Medical Institute, Depts. of Molecular and Cellular Physiology and Structural Biology, Stanford University School of Medicine, Stanford, CA.
authors.forename: K Christopher
authors.initials: KC
authors.lastname: Garcia
authors.affiliation: Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA.
authors.forename: Kai
authors.initials: K
authors.lastname: Zinn
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D004330:Drosophila / Q000254:growth & development / Q000378:metabolism*; D029721:Drosophila Proteins / Q000737:chemistry / Q000378:metabolism*; D007136:Immunoglobulins / Q000378:metabolism*; D007814:Larva / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism*; D008958:Models, Molecular; D005810:Multigene Family; D009474:Neurons / Q000378:metabolism*; D060066:Protein Interaction Maps; D016415:Sequence Alignment; D013569:Synapses
chemical_list: C454684:DPR1 protein, Drosophila; D029721:Drosophila Proteins; D007136:Immunoglobulins; D008565:Membrane Proteins
keywords: GFP tagging; Mimic; cell adhesion; immunoglobulin; optic lobe; synaptogenesis