title: Competitive fluorescence assay for specific recognition of atrazine by magnetic molecularly imprinted polymer based on Fe3O4-chitosan.
abstract: A novel fluorescence sensing strategy for determination of atrazine in tap water involving direct competition between atrazine and 5-(4,6-dichlorotriazinyl) aminofluorescein (5-DTAF), and which exploits magnetic molecularly imprinted polymer (MMIP), has been developed. The MMIP, based on Fe3O4-chitosan nanoparticles, was synthesized to recognize specific binding sites of atrazine. The recognition capability and selectivity of the MMIP for atrazine and other triazine herbicides was investigated. Under optimal conditions, the competitive reaction between 5-DTAF and atrazine was performed to permit quantitation. Fluorescence intensity changes at 515 nm was linearly related to the logarithm of the atrazine concentration for the range 2.32-185.4 μM. The detection limit for atrazine was 0.86μM (S/N=3) and recoveries were 77.6-115% in spiked tap water samples.
authors.affiliation: School of Food Science and Engineering, Harbin Institute of Technology, Harbin 150090, PR China; Key Laboratory of Agro-Product Quality and Safety, Institute of Quality Standard and Testing Technology for Agro-Product, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
authors.forename: Guangyang
authors.initials: G
authors.lastname: Liu
authors.affiliation: Key Laboratory of Agro-Product Quality and Safety, Institute of Quality Standard and Testing Technology for Agro-Product, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
authors.forename: Tengfei
authors.initials: T
authors.lastname: Li
authors.affiliation: School of Food Science and Engineering, Harbin Institute of Technology, Harbin 150090, PR China. Electronic address: yangxin@hit.edu.cn.
authors.forename: Xin
authors.initials: X
authors.lastname: Yang
authors.affiliation: Key Laboratory of Agro-Product Quality and Safety, Institute of Quality Standard and Testing Technology for Agro-Product, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
authors.forename: Yongxin
authors.initials: Y
authors.lastname: She
authors.affiliation: Key Laboratory of Agro-Product Quality and Safety, Institute of Quality Standard and Testing Technology for Agro-Product, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
authors.forename: Miao
authors.initials: M
authors.lastname: Wang
authors.affiliation: School of Food Science and Engineering, Harbin Institute of Technology, Harbin 150090, PR China; Key Laboratory of Agro-Product Quality and Safety, Institute of Quality Standard and Testing Technology for Agro-Product, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China. Electronic address: w_jing2001@126.com.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: School of Food Science and Engineering, Harbin Institute of Technology, Harbin 150090, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Agro-Product Quality and Safety, Institute of Quality Standard and Testing Technology for Agro-Product, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Wang
authors.affiliation: Key Laboratory of Agro-Product Quality and Safety, Institute of Quality Standard and Testing Technology for Agro-Product, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
authors.forename: Fen
authors.initials: F
authors.lastname: Jin
authors.affiliation: Key Laboratory of Agro-Product Quality and Safety, Institute of Quality Standard and Testing Technology for Agro-Product, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
authors.forename: Maojun
authors.initials: M
authors.lastname: Jin
authors.affiliation: Key Laboratory of Agro-Product Quality and Safety, Institute of Quality Standard and Testing Technology for Agro-Product, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
authors.forename: Hua
authors.initials: H
authors.lastname: Shao
authors.affiliation: Key Laboratory of Agro-Product Quality and Safety, Institute of Quality Standard and Testing Technology for Agro-Product, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
authors.forename: Zejun
authors.initials: Z
authors.lastname: Jiang
authors.affiliation: Key Laboratory of Agro-Product Quality and Safety, Institute of Quality Standard and Testing Technology for Agro-Product, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
authors.forename: Hailong
authors.initials: H
authors.lastname: Yu
mesh_terms: D001280:Atrazine / Q000737:chemistry*; D048271:Chitosan / Q000737:chemistry*; D052203:Ferrosoferric Oxide / Q000737:chemistry*; D005453:Fluorescence; D054802:Molecular Imprinting / Q000379:methods*; D011108:Polymers / Q000737:chemistry*
chemical_list: D011108:Polymers; D048271:Chitosan; D001280:Atrazine; D052203:Ferrosoferric Oxide
keywords: Atrazine; Fe(3)O(4)-chitosan nanoparticles; Fluorescence assay; Magnetic molecularly imprinted polymers