title: Multiomic analysis of mice epilepsy models suggest that miR-21a expression modulates mRNA and protein levels related to seizure deterioration.
abstract: Epilepsy is now recognized as the second most common neurological disease in China. To determine the genetic cause of epileptic encephalopathy, we performed a multiomics study using mouse models of controls, anticonvulsant mice treated with five drugs and epileptic mice. Based on genome-wide profiling analysis, we discovered four genes in the epileptic mouse group with differentially-expressed mRNA. After isobaric tags for relative and absolute quantification (iTRAQ) validation, only one gene, SNCA, remained, which was associated with apoptotic response of neuronal cells, and regulation of dopamine release and transport. We also identified three miRNAs targeting SNCA, out of which mmu-miR-21a-3p demonstrated a seven-fold change in expression between control and epileptic mice.
authors.affiliation: BGI Education Center,University of Chinese Academy of Science,Beishan Industrial Zone,Yantian District,Shenzhen 518083,China.
authors.forename: Xiaoxiao
authors.initials: X
authors.lastname: Hu
authors.affiliation: China-Japan Union Hospital of Jilin University,Changchun,P.R. China.
authors.forename: Xin
authors.initials: X
authors.lastname: Fu
authors.affiliation: China-Japan Union Hospital of Jilin University,Changchun,P.R. China.
authors.forename: A O
authors.initials: AO
authors.lastname: Jiang
authors.affiliation: BGI-Shenzhen,Shenzhen,518083,China.
authors.forename: Xukui
authors.initials: X
authors.lastname: Yang
authors.affiliation: BGI-Shenzhen,Shenzhen,518083,China.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Fang
authors.affiliation: Medicinal Chemistry and Pharmacology Institute,Inner Mongolia University for the Nationalities,Tongliao,Inner Mongolia,P.R. China.
authors.forename: Guohua
authors.initials: G
authors.lastname: Gong
authors.affiliation: Medicinal Chemistry and Pharmacology Institute,Inner Mongolia University for the Nationalities,Tongliao,Inner Mongolia,P.R. China.
authors.forename: Chengxi
authors.initials: C
authors.lastname: Wei
mesh_terms: D000818:Animals; D004827:Epilepsy / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D020869:Gene Expression Profiling; D008297:Male; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D051844:alpha-Synuclein / Q000235:genetics / Q000378:metabolism*
chemical_list: D035683:MicroRNAs; D051844:alpha-Synuclein; C497605:Snca protein, mouse; C531950:MIRN21 microRNA, mouse