title: Regulation of mesenchymal stem cell 3D microenvironment: From macro to microfluidic bioreactors.
abstract: Human mesenchymal stem cells (hMSCs) have emerged as an important cell type in cell therapy and tissue engineering. In these applications, maintaining the therapeutic properties of hMSCs requires tight control of the culture environments and the structural cell organizations. Bioreactor systems are essential tools to achieve these goals in the clinical-scale expansion and tissue engineering applications. This review summarizes how different bioreactors provide cues to regulate the structure and the chemico-mechanical microenvironment of hMSCs with a focus on 3D organization. In addition to conventional bioreactors, recent advances in microfluidic bioreactors as a novel approach to better control the hMSC microenvironment are also discussed. These advancements highlight the key role of bioreactor systems in preserving hMSC's functional properties by providing dynamic and temporal regulation of in vitro cellular microenvironment.
authors.affiliation: Hydrodynamics Laboratory, CNRS UMR7646, Ecole Polytechnique, Palaiseau, France.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Sart
authors.affiliation: Laboratory of Bioengineering, Catholic University of Louvain, Louvain-la-Neuve, Belgium.
authors.forename: Spiros N
authors.initials: SN
authors.lastname: Agathos
authors.affiliation: Department of Chemical and Biomedical Engineering, FAMU-FSU College of Engineering, Florida State University, Tallahassee, Florida, USA.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Chemical and Biomedical Engineering, FAMU-FSU College of Engineering, Florida State University, Tallahassee, Florida, USA. teng@eng.fsu.edu.
authors.forename: Teng
authors.initials: T
authors.lastname: Ma
mesh_terms: D019149:Bioreactors; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology; D044085:Microfluidics; D023822:Tissue Engineering
keywords: Bioreactors; Mesenchymal stem cells; Microenvironment; Microfluidics; Three-dimensional cell culture