title: Frequency of Sperm DNA Fragmentation According to Selection Method: Comparison and Relevance of a Microfluidic Device and a Swim-up Procedure.
abstract: BACKGROUND
  Multiple rounds of centrifugation or washing spermatozoa can cause sperm DNA fragmentation (SDF); however, a microfluidic approach to select spermatozoa does not require centrifugation. Reports have suggested that sperm sorting using a microfluidic device is an effective method to select good quality spermatozoa, however, it is not known whether it reduces sperm DNA damage. We investigated whether the frequency of SDF was affected by selection method during sperm processing.
  
  
  METHODS
  Semen samples from ten men with normal, oligozoospermia and asthenozoospermia were split into two groups and sorted using a microfluidic device or by a swim-up method. Subsequently, semen parameters and SDF were measured and analyzed using paired or non-paired Student's t-tests.
  
  
  RESULTS
  For samples sorted by the microfluidic device (Sperm Sorter Qualis(®); Menicon, Kasugai, Japan) or the swim-up method, both showed a decrease in SDF. However, the decrease was more significant when the microfluidic device was used.
  
  
  CONCLUSIONS
  Sorting using the microfluidic device resulted in less SDF than did the swim-up method.
authors.affiliation: Embryologist, Hanabusa Women's Clinic , Sannomiya, Kobe, Japan .
authors.forename: Kanako
authors.initials: K
authors.lastname: Kishi
authors.affiliation: Director of Embryologist, Hanabusa Women's Clinic , Sannomiya, Kobe, Japan .
authors.forename: Hiromi
authors.initials: H
authors.lastname: Ogata
authors.affiliation: PhD, Hanabusa Women's Clinic , Sannomiya, Kobe, Japan .
authors.forename: Seiji
authors.initials: S
authors.lastname: Ogata
authors.affiliation: PhD, Hanabusa Women's Clinic , Sannomiya, Kobe, Japan .
authors.forename: Yuri
authors.initials: Y
authors.lastname: Mizusawa
authors.affiliation: Hanabusa Women's Clinic , Sannomiya, Kobe, Japan .
authors.forename: Eri
authors.initials: E
authors.lastname: Okamoto
authors.affiliation: Hanabusa Women's Clinic , Sannomiya, Kobe, Japan .
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Matsumoto
authors.affiliation: PhD, Hanabusa Women's Clinic , Sannomiya, Kobe, Japan .
authors.forename: Shoji
authors.initials: S
authors.lastname: Kokeguchi
authors.affiliation: PhD, CEO and Chief of Medical Clinic, Hanabusa Women's Clinic , Sannomiya, Kobe, Japan .
authors.forename: Masahide
authors.initials: M
authors.lastname: Shiotani
keywords: Sperm concentration; Sperm selection; Spermatozoa