title: MicroRNA expression profiles in pediatric dysembryoplastic neuroepithelial tumors.
abstract: Among noncoding RNAs, microRNAs (miRNAs) have been most extensively studied, and their biology has repeatedly been proven critical for central nervous system pathological conditions. The diagnostic value of several miRNAs was appraised in pediatric dysembryoplastic neuroepithelial tumors (DNETs) using miRNA microarrays and receiving operating characteristic curves analyses. Overall, five pediatric DNETs were studied. As controls, 17 samples were used: the FirstChoice Human Brain Reference RNA and 16 samples from deceased children who underwent autopsy and were not present with any brain malignancy. The miRNA extraction was carried out using the mirVANA miRNA Isolation Kit, while the experimental approach included miRNA microarrays covering 1211 miRNAs. Quantitative real-time polymerase chain reaction was performed to validate the expression profiles of miR-1909* and miR-3138 in all samples initially screened with miRNA microarrays. Our findings indicated that miR-3138 might act as a tumor suppressor gene when down-regulated and miR-1909* as a putative oncogenic molecule when up-regulated in pediatric DNETs compared to the control cohort. Subsequently, both miRNA signatures might serve as putative diagnostic biomarkers for pediatric DNETs.
authors.affiliation: University Research Institute for the Study and Treatment of Childhood Genetic and Malignant Diseases, "Aghia Sophia" Children's Hospital, University of Athens, Athens, Greece. mbraoudak@med.uoa.gr.|First Department of Pediatrics, Hematology and Oncology Unit, Choremeio Research Laboratory, "Aghia Sophia" Children's Hospital, University of Athens Medical School, Thivon and Levadias, 11527, Goudi, Athens, Greece. mbraoudak@med.uoa.gr.
authors.forename: M
authors.initials: M
authors.lastname: Braoudaki
authors.affiliation: University Research Institute for the Study and Treatment of Childhood Genetic and Malignant Diseases, "Aghia Sophia" Children's Hospital, University of Athens, Athens, Greece.
authors.forename: G I
authors.initials: GI
authors.lastname: Lambrou
authors.affiliation: Department of Forensic Medicine and Toxicology, School of Medicine, University of Athens, Athens, Greece.
authors.forename: S A
authors.initials: SA
authors.lastname: Papadodima
authors.affiliation: Department of Pathology, Children's Hospital "Aghia Sophia", Athens, Greece.
authors.forename: K
authors.initials: K
authors.lastname: Stefanaki
authors.affiliation: Department of Neurosurgery, Children's Hospital "Aghia Sophia", Athens, Greece.
authors.forename: N
authors.initials: N
authors.lastname: Prodromou
authors.affiliation: University Research Institute for the Study and Treatment of Childhood Genetic and Malignant Diseases, "Aghia Sophia" Children's Hospital, University of Athens, Athens, Greece.
authors.forename: E
authors.initials: E
authors.lastname: Kanavakis
mesh_terms: D019540:Area Under Curve; D014408:Biomarkers, Tumor / Q000032:analysis / Q000235:genetics*; D001932:Brain Neoplasms / Q000235:genetics*; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000032:analysis / Q000096:biosynthesis*; D018302:Neoplasms, Neuroepithelial / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D012372:ROC Curve; D060888:Real-Time Polymerase Chain Reaction; D059467:Transcriptome
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs; C000610440:microRNA1909 microRNA, human; C000610441:microRNA3138 microRNA, human
keywords: Biomarkers; Brain tumors; MicroRNA microarrays; Pediatric; ROC curves; qRT-PCR