title: Ig Superfamily Ligand and Receptor Pairs Expressed in Synaptic Partners in Drosophila.
abstract: Information processing relies on precise patterns of synapses between neurons. The cellular recognition mechanisms regulating this specificity are poorly understood. In the medulla of the Drosophila visual system, different neurons form synaptic connections in different layers. Here, we sought to identify candidate cell recognition molecules underlying this specificity. Using RNA sequencing (RNA-seq), we show that neurons with different synaptic specificities express unique combinations of mRNAs encoding hundreds of cell surface and secreted proteins. Using RNA-seq and protein tagging, we demonstrate that 21 paralogs of the Dpr family, a subclass of immunoglobulin (Ig)-domain containing proteins, are expressed in unique combinations in homologous neurons with different layer-specific synaptic connections. Dpr interacting proteins (DIPs), comprising nine paralogs of another subclass of Ig-containing proteins, are expressed in a complementary layer-specific fashion in a subset of synaptic partners. We propose that pairs of Dpr/DIP paralogs contribute to layer-specific patterns of synaptic connectivity.
authors.affiliation: Department of Biological Chemistry, HHMI, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095, USA.
authors.forename: Liming
authors.initials: L
authors.lastname: Tan
authors.affiliation: Department of Biological Chemistry, HHMI, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095, USA.
authors.forename: Kelvin Xi
authors.initials: KX
authors.lastname: Zhang
authors.affiliation: Department of Biological Chemistry, HHMI, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095, USA.
authors.forename: Matthew Y
authors.initials: MY
authors.lastname: Pecot
authors.affiliation: Department of Molecular and Human Genetics, HHMI, Baylor College of Medicine, Houston, TX 77030, USA.
authors.forename: Sonal
authors.initials: S
authors.lastname: Nagarkar-Jaiswal
authors.affiliation: Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA.
authors.forename: Pei-Tseng
authors.initials: PT
authors.lastname: Lee
authors.affiliation: Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA.
authors.forename: Shin-Ya
authors.initials: SY
authors.lastname: Takemura
authors.affiliation: Department of Biological Chemistry, HHMI, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095, USA.
authors.forename: Jason M
authors.initials: JM
authors.lastname: McEwen
authors.affiliation: Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA.
authors.forename: Aljoscha
authors.initials: A
authors.lastname: Nern
authors.affiliation: Department of Biological Chemistry, HHMI, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095, USA.
authors.forename: Shuwa
authors.initials: S
authors.lastname: Xu
authors.affiliation: Department of Biological Chemistry, HHMI, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095, USA.
authors.forename: Wael
authors.initials: W
authors.lastname: Tadros
authors.affiliation: Department of Biology, New York University, 100 Washington Square East, New York, NY 10003, USA.
authors.forename: Zhenqing
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
authors.forename: Kai
authors.initials: K
authors.lastname: Zinn
authors.affiliation: Department of Molecular and Human Genetics, HHMI, Baylor College of Medicine, Houston, TX 77030, USA; Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA.
authors.forename: Hugo J
authors.initials: HJ
authors.lastname: Bellen
authors.affiliation: Departament de Genètica, Facultat de Biologia and Institut de Biomedicina (IBUB) de la Universitat de Barcelona, Barcelona 08028, Spain. Electronic address: mmorey@ub.edu.
authors.forename: Marta
authors.initials: M
authors.lastname: Morey
authors.affiliation: Department of Biological Chemistry, HHMI, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095, USA. Electronic address: lzipursky@mednet.ucla.edu.
authors.forename: S Lawrence
authors.initials: SL
authors.lastname: Zipursky
mesh_terms: D000818:Animals; D004330:Drosophila; D029721:Drosophila Proteins / Q000378:metabolism*; D005434:Flow Cytometry; D007136:Immunoglobulins / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D011971:Receptors, Immunologic / Q000378:metabolism*; D017423:Sequence Analysis, RNA; D013569:Synapses; D014785:Vision, Ocular
chemical_list: C454684:DPR1 protein, Drosophila; D029721:Drosophila Proteins; D007136:Immunoglobulins; D011971:Receptors, Immunologic
keywords: DIP; Dpr; Drosophila; synaptic specificity; visual system