title: Conversion of a non-heme iron-dependent sulfoxide synthase into a thiol dioxygenase by a single point mutation.
abstract: EgtB from Mycobacterium thermoresistibile catalyzes O2-dependent sulfur-carbon bond formation between the side chains of Nα-trimethyl histidine and γ-glutamyl cysteine as a central step in ergothioneine biosynthesis. A single point mutation converts this enzyme into a γ-glutamyl cysteine dioxygenase with an efficiency that rivals naturally evolved thiol dioxygenases.
authors.affiliation: Department for Chemistry, University of Basel, St. Johanns-Ring 19, 4056, Basel, Switzerland. florian.seebeck@unibas.ch.
authors.forename: Kristina V
authors.initials: KV
authors.lastname: Goncharenko
authors.affiliation: Department for Chemistry, University of Basel, St. Johanns-Ring 19, 4056, Basel, Switzerland. florian.seebeck@unibas.ch.
authors.forename: Florian P
authors.initials: FP
authors.lastname: Seebeck
mesh_terms: D020134:Catalytic Domain; D049308:Dioxygenases / Q000737:chemistry* / Q000235:genetics; D007501:Iron / Q000737:chemistry*; D017354:Point Mutation
chemical_list: D007501:Iron; D049308:Dioxygenases