title: Facile preparation of mesoporous carbon-silica-coated graphene for the selective enrichment of endogenous peptides.
abstract: A sandwich-like composite composed of ordered mesoporous carbon-silica shell-coated graphene (denoted as graphene@mSiO2-C) was prepared by an in-situ carbonation strategy. A mesoporous silica shell was synthesized by a sol-gel method, and cetyltrimethyl ammonium bromide inside the mesopores were in-situ carbonized as a carbon source to obtain a carbon-silica shell. The resulting mesoporous carbon-silica material with a sandwich structure possesses a high surface area (600 m(2) g(-1)), large pore volume (0.587 cm(3) g(-1)), highly ordered mesoporous pore (3 nm), and high carbon content (30%). This material shows not only high hydrophobicity of graphene and mesoporous carbon but also a hydrophilic silica framework that ensures excellent dispersibility in aqueous solution. The material can capture many more peptides from bovine serum albumin tryptic digests than mesoporous silica shell-coated graphene, demonstrating great enrichment efficiency for peptides. Furthermore, the prepared composite was applied to the enrichment of low-abundance endogenous peptides in human serum. Based on Matrix-Assisted Laser Desorption/ Ionization Time of Flight Mass Spectrometry identification, the graphene@mSiO2-C could efficiently size-exclude proteins and enriches the low-abundant peptides on the graphene and mesoporous carbon. And based on the LC-MS/MS results, 892 endogenous peptides were obtained by graphene@mSiO2-C, hinting at its great potential in peptides analysis.
authors.affiliation: Division of Metrology in Chemistry, National Institute of Metrology, Beijing 100029, PR China; CAS Key Laboratory of Separation Sciences for Analytical Chemistry, National Chromatographic R & A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Dalian 116023, PR China.
authors.forename: Quanqing
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Division of Metrology in Chemistry, National Institute of Metrology, Beijing 100029, PR China. Electronic address: qhzhang204@gmail.com.
authors.forename: Qinghe
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: CAS Key Laboratory of Separation Sciences for Analytical Chemistry, National Chromatographic R & A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Dalian 116023, PR China.
authors.forename: Zhichao
authors.initials: Z
authors.lastname: Xiong
authors.affiliation: CAS Key Laboratory of Separation Sciences for Analytical Chemistry, National Chromatographic R & A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Dalian 116023, PR China.
authors.forename: Hao
authors.initials: H
authors.lastname: Wan
authors.affiliation: Division of Metrology in Chemistry, National Institute of Metrology, Beijing 100029, PR China.
authors.forename: Xiaoting
authors.initials: X
authors.lastname: Chen
authors.affiliation: Division of Metrology in Chemistry, National Institute of Metrology, Beijing 100029, PR China.
authors.forename: Hongmei
authors.initials: H
authors.lastname: Li
authors.affiliation: CAS Key Laboratory of Separation Sciences for Analytical Chemistry, National Chromatographic R & A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Dalian 116023, PR China. Electronic address: hanfazou@dicp.ac.cn.
authors.forename: Hanfa
authors.initials: H
authors.lastname: Zou
mesh_terms: D000818:Animals; D002417:Cattle; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000737:chemistry; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D010455:Peptides / Q000737:chemistry*; D016062:Porosity; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism; D012822:Silicon Dioxide / Q000737:chemistry*; D014357:Trypsin / Q000378:metabolism
chemical_list: D002593:Cetrimonium Compounds; D010455:Peptides; D012710:Serum Albumin, Bovine; D012822:Silicon Dioxide; D006108:Graphite; D014357:Trypsin; D000077286:Cetrimonium
keywords: Endogenous peptides; Graphene; In-situ carbonization; Mesoporous carbon; Sandwich-like composite; Size exclusion