title: XBP1 silencing decreases glioma cell viability and glycolysis possibly by inhibiting HK2 expression.
abstract: Glioma cells rely on glycolysis to obtain energy and sustain their survival under microenvironmental stress in vivo. The mechanisms of regulation of glycolysis in glioma cells are unclear. Signaling pathway mediated by the transcription factor X box-binding protein 1 (XBP1) is one of the most important pathways of unfolded protein response which is comprehensively activated in cancer cells upon the microenvironmental stress. Here we showed that XBP1 was significantly activated in glioma tissues in vivo. XBP1 silencing resulted in decreasing of glioma cell viability and ATP/lactate production under hypoxia, which is possibly mediated by inhibition of Hexokinase II (HK2)'s expression. More importantly, XBP1 silenced glioma cells showed the decrease of tumor formation capacity. Our results revealed that XBP1s activation was involved in glioma glycolysis regulation and might be a potential molecular target for glioma treatment.
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, People's Republic of China.
authors.forename: Yaohua
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, People's Republic of China.
authors.forename: Xu
authors.initials: X
authors.lastname: Hou
authors.affiliation: College of Basic Medicine, Beijing University of Chinese Medicine, Beijing, People's Republic of China.
authors.forename: Min
authors.initials: M
authors.lastname: Liu
authors.affiliation: Department of Endocrinology, First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, People's Republic of China.
authors.forename: Zhuowen
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, People's Republic of China.
authors.forename: Yunke
authors.initials: Y
authors.lastname: Bi
authors.affiliation: Department of Pain Medicine, Cancer Hospital of Harbin Medical University, Harbin, Heilongjiang Province, People's Republic of China.
authors.forename: Huichao
authors.initials: H
authors.lastname: Zou
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, People's Republic of China.
authors.forename: Jianing
authors.initials: J
authors.lastname: Wu
authors.affiliation: Department of Pharmacology (State-Province Key Laboratories of Biomedicine-Pharmaceutics of China), Harbin Medical University, Harbin, Heilongjiang Province, People's Republic of China.
authors.forename: Hui
authors.initials: H
authors.lastname: Che
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, People's Republic of China.
authors.forename: Chenguang
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, People's Republic of China.
authors.forename: Xiaoxiong
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, People's Republic of China.
authors.forename: Kaikai
authors.initials: K
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, People's Republic of China.
authors.forename: Chen
authors.initials: C
authors.lastname: Zhong
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, People's Republic of China.
authors.forename: Jiakang
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, People's Republic of China.
authors.forename: Tao
authors.initials: T
authors.lastname: Yu
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, People's Republic of China.
authors.forename: Qilong
authors.initials: Q
authors.lastname: Bian
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, People's Republic of China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Chai
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, People's Republic of China.
authors.forename: Huailei
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Pharmacology (State-Province Key Laboratories of Biomedicine-Pharmaceutics of China), Harbin Medical University, Harbin, Heilongjiang Province, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Ai
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, People's Republic of China. guangsz@hotmail.com.
authors.forename: Shiguang
authors.initials: S
authors.lastname: Zhao
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D017209:Apoptosis; D049109:Cell Proliferation; D004268:DNA-Binding Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D005260:Female; D020868:Gene Silencing; D005910:Glioma; D006019:Glycolysis; D006593:Hexokinase / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D006801:Humans; D000860:Hypoxia / Q000503:physiopathology; D019344:Lactic Acid / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D010101:Oxygen Consumption; D034741:RNA, Small Interfering / Q000235:genetics*; D017208:Rats, Wistar; D000071701:Regulatory Factor X Transcription Factors; D015398:Signal Transduction; D014157:Transcription Factors / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D014407:Tumor Cells, Cultured; D000071717:X-Box Binding Protein 1; D023041:Xenograft Model Antitumor Assays
chemical_list: D004268:DNA-Binding Proteins; D034741:RNA, Small Interfering; D000071701:Regulatory Factor X Transcription Factors; D014157:Transcription Factors; D000071717:X-Box Binding Protein 1; C000605543:XBP1 protein, human; C000605544:Xbp1 protein, mouse; C000605545:Xbp1 protein, rat; D019344:Lactic Acid; D000255:Adenosine Triphosphate; D006593:Hexokinase
keywords: Cancer metabolism; Glioma; HK2; XBP1