title: Pattern characterization of genes involved in non-specific immune response in Staphylococcus aureus isolates from intramammary infections.
abstract: Staphylococcus aureus isolated from mammary gland are characterized by different genetic patterns. Ninety four isolates from 33 dairy herds were analyzed by the means of a microarray to investigate S. aureus virulence patterns and the distribution of genes believed to be involved in immune evasion. None of the 94 isolates considered were MRSA. However, 50% of the isolates belonged to complexes related to MRSA and to human diseases, while only about 25% of them can be considered as exclusively of bovine origin. The distribution of clonal complexes and the different gene patterns observed confirmed the presence of an influence of geographical localization. The assessment of the influence of genes related to immune evasion on quarter milk cell count showed as four of them showed to be significantly associated to an increase quarter milk SCC. These genes could be potential target for developing new vaccines against S. aureus.
authors.affiliation: Dept. Animal Pathology, Hygiene and Public Health, Università degli Studi di Milano, Via Celoria 10, 20133 Milano, Italy.
authors.forename: Maria
authors.initials: M
authors.lastname: Mazzilli
authors.affiliation: Dept. Animal Pathology, Hygiene and Public Health, Università degli Studi di Milano, Via Celoria 10, 20133 Milano, Italy.
authors.forename: Renata
authors.initials: R
authors.lastname: Piccinini
authors.affiliation: Dept. Animal Pathology, Hygiene and Public Health, Università degli Studi di Milano, Via Celoria 10, 20133 Milano, Italy.
authors.forename: Federico
authors.initials: F
authors.lastname: Scali
authors.affiliation: Dept. Animal Pathology, Hygiene and Public Health, Università degli Studi di Milano, Via Celoria 10, 20133 Milano, Italy. Electronic address: alfonso.zecconi@unimi.it.
authors.forename: Alfonso
authors.initials: A
authors.lastname: Zecconi
mesh_terms: D000818:Animals; D002417:Cattle; D005260:Female; D007113:Immunity, Innate; D007558:Italy; D008414:Mastitis, Bovine / Q000276:immunology* / Q000382:microbiology; D058885:Multilocus Sequence Typing / Q000662:veterinary; D013203:Staphylococcal Infections / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D013211:Staphylococcus aureus / Q000235:genetics* / Q000276:immunology
keywords: Clonal complex; Enterotoxins; Epidemiology; Immune evasion; Mastitis; Staphylococcus aureus