title: Impact of Porosity and Electrolyte Composition on the Surface Charge of Hydroxyapatite Biomaterials.
abstract: The success or failure of a material when implanted in the body is greatly determined by the surface properties of the material and the host tissue reactions. The very first event that takes place after implantation is the interaction of soluble ions, molecules and proteins from the biological environment with the material surface leading to the formation of an adsorbed protein layer that will later influence cell attachment. In this context, the particular topography and surface charge of a material become critical as they influence the nature of the proteins that will adsorb. However, very limited information is available on the surface charge of porous substrates. Only until very recently was the determination of the zeta potential on porous membranes accurately determined. The goal of this work was to implement the previous findings for the determination of the zeta potential of a series of porous hydroxyapatite (HA) substrates and to assess how porosity affects the measurements. In addition, studies using various electrolytes were also performed to prove how the specific affinity of certain ions for HA can further impact surface charge. The results showed that all materials exhibited very similar external surface charge (approximately -23 mV), consistent with their almost identical topographies. However, the presence of interconnected pores underneath the sample surface resulted in an additional internal zeta potential that varied with the porosity content. Measurements with different electrolytes confirmed the selectivity of divalent ions for HA underlying the importance of testing biomaterials using relevant electrolytes.
authors.affiliation: Biomaterials, Biomechanics and Tissue Engineering Group, Department of Materials Science and Metallurgical Engineering, Technical University of Catalonia (UPC) , Avinguda Diagonal 647, 08028 Barcelona, Spain.|Centre for Research in Nanoengineering, Technical University of Catalonia (CRNE-UPC) , Pascual i Vila 15, 08028 Barcelona, Spain.
authors.forename: Montserrat
authors.initials: M
authors.lastname: Espanol
authors.affiliation: Department of Engineering Sciences, Uppsala University , 751 21 Uppsala, Sweden.
authors.forename: Gemma
authors.initials: G
authors.lastname: Mestres
authors.affiliation: Anton Paar GmbH , 8054 Graz, Austria.
authors.forename: Thomas
authors.initials: T
authors.lastname: Luxbacher
authors.affiliation: Biomaterials, Biomechanics and Tissue Engineering Group, Department of Materials Science and Metallurgical Engineering, Technical University of Catalonia (UPC) , Avinguda Diagonal 647, 08028 Barcelona, Spain.
authors.forename: Jean-Baptiste
authors.initials: JB
authors.lastname: Dory
authors.affiliation: Biomaterials, Biomechanics and Tissue Engineering Group, Department of Materials Science and Metallurgical Engineering, Technical University of Catalonia (UPC) , Avinguda Diagonal 647, 08028 Barcelona, Spain.|Centre for Research in Nanoengineering, Technical University of Catalonia (CRNE-UPC) , Pascual i Vila 15, 08028 Barcelona, Spain.
authors.forename: Maria-Pau
authors.initials: MP
authors.lastname: Ginebra
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D017886:Durapatite / Q000737:chemistry*; D004553:Electric Conductivity; D004573:Electrolytes / Q000737:chemistry*; D008628:Mercury / Q000737:chemistry; D016062:Porosity; D055672:Static Electricity; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D004573:Electrolytes; D017886:Durapatite; D008628:Mercury
keywords: bone substitute ion adsorption; hydroxyapatite; porosity; streaming current; surface charge; texture; zeta potential