title: High-content analysis of Rab protein function at the ER-Golgi interface.
abstract: The Rab family of small GTPases play fundamental roles in the regulation of trafficking pathways between intracellular membranes in eukaryotic cells. In this short commentary we highlight a recent high-content screening study that investigates the roles of Rab proteins in retrograde trafficking from the Golgi complex to the endoplasmic reticulum, and we discuss how the findings of this work and other literature might influence our thoughts on how the architecture of the Golgi complex is regulated.
authors.affiliation: a School of Biology and Environmental Science & UCD Conway Institute of Biomolecular and Biomedical Research; University College Dublin ; Dublin , Ireland.
authors.forename: George
authors.initials: G
authors.lastname: Galea
authors.affiliation: a School of Biology and Environmental Science & UCD Conway Institute of Biomolecular and Biomedical Research; University College Dublin ; Dublin , Ireland.
authors.forename: Jeremy C
authors.initials: JC
authors.lastname: Simpson
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D006056:Golgi Apparatus / Q000378:metabolism*; D006801:Humans; D021381:Protein Transport / Q000502:physiology*; D020691:rab GTP-Binding Proteins / Q000378:metabolism*
chemical_list: D020691:rab GTP-Binding Proteins
keywords: ER-Golgi interface; Golgi morphology; Rab proteins; high-content screening; retrograde traffic