title: A Simple Microfluidic Platform for Long-Term Analysis and Continuous Dual-Imaging Detection of T-Cell Secreted IFN-γ and IL-2 on Antibody-Based Biochip.
abstract: The identification and characterization, at the cellular level, of cytokine productions present a high interest for both fundamental research and clinical studies. However, the majority of techniques currently available (ELISA, ELISpot, flow cytometry, etc.) have several shortcomings including, notably, the assessment of several cytokines in relation to individual secreting cells and the monitoring of living cell responses for a long incubation time. In the present work, we describe a system composed of a microfluidic platform coupled with an antibody microarray chip for continuous SPR imaging and immunofluorescence analysis of cytokines (IL-2 and IFN-γ) secreted by T-Lymphocytes, specifically, and stably captured on the biochip under flow upon continued long-term on-chip culture (more than 24 h).
authors.affiliation: University of Grenoble Alpes, INAC-SPRAM, Grenoble F-38000, France. marchep@ujf-grenoble.fr.|CEA, INAC-SPrAM, Grenoble F-38000, France. marchep@ujf-grenoble.fr.|CNRS, INAC-SPrAM, Grenoble F-38000, France. marchep@ujf-grenoble.fr.|University Grenoble Alpes, Institut Albert Bonniot, Grenoble F-38000, France. marchep@ujf-grenoble.fr.|INSERM U823, Grenoble F-38000, France. marchep@ujf-grenoble.fr.
authors.forename: Dieudonné R
authors.initials: DR
authors.lastname: Baganizi
authors.affiliation: University of Grenoble Alpes, INAC-SPRAM, Grenoble F-38000, France. loic.leroy@ujf-grenoble.fr.|CEA, INAC-SPrAM, Grenoble F-38000, France. loic.leroy@ujf-grenoble.fr.|CNRS, INAC-SPrAM, Grenoble F-38000, France. loic.leroy@ujf-grenoble.fr.
authors.forename: Loïc
authors.initials: L
authors.lastname: Leroy
authors.affiliation: University of Grenoble Alpes, INAC-SPRAM, Grenoble F-38000, France. loic.leroy@ujf-grenoble.fr.|CEA, INAC-SPrAM, Grenoble F-38000, France. loic.leroy@ujf-grenoble.fr.|CNRS, INAC-SPrAM, Grenoble F-38000, France. loic.leroy@ujf-grenoble.fr.
authors.forename: Loïc
authors.initials: L
authors.lastname: Laplatine
authors.affiliation: University of Grenoble Alpes, INAC-SPRAM, Grenoble F-38000, France. sjfairley@hotmail.com.|CEA, INAC-SPrAM, Grenoble F-38000, France. sjfairley@hotmail.com.|CNRS, INAC-SPrAM, Grenoble F-38000, France. sjfairley@hotmail.com.
authors.forename: Stacie J
authors.initials: SJ
authors.lastname: Fairley
authors.affiliation: University of Grenoble Alpes, INAC-SPRAM, Grenoble F-38000, France. samuel.heidmann@cea.fr.|CEA, INAC-SPrAM, Grenoble F-38000, France. samuel.heidmann@cea.fr.|CNRS, INAC-SPrAM, Grenoble F-38000, France. samuel.heidmann@cea.fr.
authors.forename: Samuel
authors.initials: S
authors.lastname: Heidmann
authors.affiliation: University of Grenoble Alpes, INAC-SPRAM, Grenoble F-38000, France. samia.menad@cea.fr.|CEA, INAC-SPrAM, Grenoble F-38000, France. samia.menad@cea.fr.|CNRS, INAC-SPrAM, Grenoble F-38000, France. samia.menad@cea.fr.
authors.forename: Samia
authors.initials: S
authors.lastname: Menad
authors.affiliation: University of Grenoble Alpes, INAC-SPRAM, Grenoble F-38000, France. thierry.livache@cea.fr.|CEA, INAC-SPrAM, Grenoble F-38000, France. thierry.livache@cea.fr.|CNRS, INAC-SPrAM, Grenoble F-38000, France. thierry.livache@cea.fr.
authors.forename: Thierry
authors.initials: T
authors.lastname: Livache
authors.affiliation: University Grenoble Alpes, Institut Albert Bonniot, Grenoble F-38000, France. marchep@ujf-grenoble.fr.|INSERM U823, Grenoble F-38000, France. marchep@ujf-grenoble.fr.
authors.forename: Patrice N
authors.initials: PN
authors.lastname: Marche
authors.affiliation: University of Grenoble Alpes, INAC-SPRAM, Grenoble F-38000, France. Yoann.Roupioz@cea.fr.|CEA, INAC-SPrAM, Grenoble F-38000, France. Yoann.Roupioz@cea.fr.|CNRS, INAC-SPrAM, Grenoble F-38000, France. Yoann.Roupioz@cea.fr.
authors.forename: Yoann
authors.initials: Y
authors.lastname: Roupioz
mesh_terms: D000328:Adult; D055910:Antibodies, Immobilized / Q000737:chemistry* / Q000276:immunology; D002470:Cell Survival; D004867:Equipment Design; D006801:Humans; D007371:Interferon-gamma / Q000032:analysis* / Q000276:immunology; D007376:Interleukin-2 / Q000032:analysis* / Q000276:immunology; D056656:Lab-On-A-Chip Devices; D040081:Protein Array Analysis / Q000295:instrumentation; D020349:Surface Plasmon Resonance / Q000295:instrumentation*; D013601:T-Lymphocytes / Q000737:chemistry / Q000276:immunology*
chemical_list: D055910:Antibodies, Immobilized; D007376:Interleukin-2; D007371:Interferon-gamma
keywords: Cytokine; PDMS; SPR imaging; T-Lymphocytes; antibody; biochip; microfluidics; self-assembled monolayers