title: The dark side of ID8-Luc2: pitfalls for luciferase tagged murine models for ovarian cancer.
abstract: Reliable mouse models are key in the discovery and development of novel anticancer treatments. Non-invasive monitoring techniques such as bioluminescence imaging (BLI) are useful tools to determine tumor engraftment and evaluate tumor growth. However, the development of ascites in ovarian cancer mouse models leads to possible difficulties. Ascites can interfere with the set-up of correct end points and can interfere with the evaluation of tumor volume using BLI. We provide optimized euthanasia criteria and in vivo data underlining the pitfalls of BLI.
authors.affiliation: Department of Gynaecology and Obstetrics, UZ Leuven, Leuven, Belgium ; Department of Oncology, Laboratory of Gynaecologic Oncology, KU Leuven, Leuven Cancer Institute, Leuven, Belgium.
authors.forename: Thaïs
authors.identifier: 0000-0001-7207-1519
authors.initials: T
authors.lastname: Baert
authors.affiliation: Department of Neuroscience, Laboratory of Experimental Neurosurgery, KU Leuven, Leuven, Belgium.
authors.forename: Tina
authors.initials: T
authors.lastname: Verschuere
authors.affiliation: Department of Oncology, Laboratory of Gynaecologic Oncology, KU Leuven, Leuven Cancer Institute, Leuven, Belgium.
authors.forename: Anaïs
authors.initials: A
authors.lastname: Van Hoylandt
authors.affiliation: Department of Pharmaceutical and Pharmacological Sciences, Laboratory of Molecular Virology and Gene Therapy and Leuven Viral Vector Core, KU Leuven, Leuven, Belgium.
authors.forename: Rik
authors.initials: R
authors.lastname: Gijsbers
authors.affiliation: Department of Gynaecology and Obstetrics, UZ Leuven, Leuven, Belgium ; Department of Oncology, Laboratory of Gynaecologic Oncology, KU Leuven, Leuven Cancer Institute, Leuven, Belgium.
authors.forename: Ignace
authors.initials: I
authors.lastname: Vergote
authors.affiliation: Department of Gynaecology and Obstetrics, UZ Leuven, Leuven, Belgium ; Department of Oncology, Laboratory of Gynaecologic Oncology, KU Leuven, Leuven Cancer Institute, Leuven, Belgium.
authors.forename: An
authors.initials: A
authors.lastname: Coosemans
keywords: Ascites; Bioluminescence imaging; ID8; Mouse model; Ovarian cancer