title: Quantum dot-DNA aptamer conjugates coupled with capillary electrophoresis: A universal strategy for ratiometric detection of organophosphorus pesticides.
abstract: Based on the highly sensitivity and stable-fluorescence of water-soluble CdTe/CdS core-shell quantum dots (QDs) with broad-specificity DNA aptamers, a novel ratiometric detection strategy was proposed for the sensitive detection of organophosphorus pesticides by capillary electrophoresis with laser-induced fluorescence (CE-LIF). The as-prepared QDs were first conjugated with the amino-modified oligonucleotide (AMO) by amidation reaction, which is partial complementary to the DNA aptamer of organophosphorus pesticides. Then QD-labeled AMO (QD-AMO) was incubated with the DNA aptamer to form QD-AMO-aptamer duplex. When the target organophosphorus pesticides were added, they could specifically bind the DNA aptamer, leading to the cleavage of QD-AMO-aptamer duplex, accompany with the release of QD-AMO. As a result, the ratio of peak height between QD-AMO and QD-AMO-aptamer duplex changed in the detection process of CE-LIF. This strategy was subsequently applied for the detection of phorate, profenofos, isocarbophos, and omethoate with the detection limits of 0.20, 0.10, 0.17, and 0.23μM, respectively. This is the first report about using QDs as the signal indicators for organophosphorus pesticides detection based on broad-specificity DNA aptamers by CE-LIF, thus contributing to extend the scope of application of QDs in different fields. The proposed method has great potential to be a universal strategy for rapid detection of aptamer-specific small molecule targets by simply changing the types of aptamer sequences.
authors.affiliation: School of Ecological and Environmental Sciences, East China Normal University, 500 Dongchuan Road, Shanghai 200241, PR China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Tang
authors.affiliation: School of Ecological and Environmental Sciences, East China Normal University, 500 Dongchuan Road, Shanghai 200241, PR China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Deng
authors.affiliation: School of Chemistry and Molecular Engineering, East China Normal University, 500 Dongchuan Road, Shanghai 200241 PR China. Electronic address: mzhang@chem.ecnu.edu.cn.
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.affiliation: School of Chemistry and Molecular Engineering, East China Normal University, 500 Dongchuan Road, Shanghai 200241 PR China.
authors.forename: Guoyue
authors.initials: G
authors.lastname: Shi
authors.affiliation: School of Ecological and Environmental Sciences, East China Normal University, 500 Dongchuan Road, Shanghai 200241, PR China. Electronic address: tszhou@des.ecnu.edu.cn.
authors.forename: Tianshu
authors.initials: T
authors.lastname: Zhou
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry* / Q000235:genetics; D001483:Base Sequence; D015374:Biosensing Techniques / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods*; D005638:Fruit / Q000737:chemistry; D007834:Lasers; D008670:Metals / Q000737:chemistry; D009943:Organophosphorus Compounds / Q000032:analysis* / Q000737:chemistry; D010575:Pesticides / Q000032:analysis* / Q000737:chemistry*; D045663:Quantum Dots / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D052157:Aptamers, Nucleotide; D008670:Metals; D009943:Organophosphorus Compounds; D010575:Pesticides
keywords: Aptamer; CE–LIF; Organophosphorus pesticides; Quantum dot; Ratiometric detection