title: Effects of Marsdenia tenacissima polysaccharide on the immune regulation and tumor growth in H22 tumor-bearing mice.
abstract: One water-soluble polysaccharide (Marsdenia tenacissima polysaccharide, MTP), with an average molecular weight of 4.9 × 10(4) Da, was isolated from the dried rattan of M. tenacissima. MTP contained 93.8% carbohydrates, 5.6% proteins and 21.3% uronic acid, and were composed of arabinose, mannose, galactose, xylose, glucuronic acid at a molar ratio of 9.1, 17.7, 30.2, 22.4 and 20.6. The experiments on the animals showed that MTP could increase the serum hemolysin, promote the formation of antibody-forming cells and improve the phagocytosis of mononuclear macrophage in normal mice. Meanwhile, MTP could also inhibit the growth of tumor in H22 tumor-bearing mice dose-dependently, and increase the spleen index, thymus index and serum albumin level in the mice. In addition, MTP could elevate the serum level of TNF-α and IL-2, increase the activity of GSH-Px, CAT and SOD in the liver tissue, and reduce the content of VEGF and MDA. These results suggest that MTP can regulate the immune function in mice and suppress the growth of tumor in H22 tumor-bearing mice, and its antitumor activity may be related to its antioxidant and immunomodulatory effects.
authors.affiliation: College of Basic Medical Sciences, Changchun University of Chinese Medicine, Changchun 130117, Jilin, PR China. Electronic address: jiangshuang_2000@163.com.
authors.forename: Shuang
authors.initials: S
authors.lastname: Jiang
authors.affiliation: Qianwei Hospital, Changchun 130021, Jilin, PR China.
authors.forename: Limin
authors.initials: L
authors.lastname: Qiu
authors.affiliation: Animal Science and Technology College, Jilin Agricultural University, Changchun 130117, Jilin, PR China.
authors.forename: Yiquan
authors.initials: Y
authors.lastname: Li
authors.affiliation: College of Basic Medical Sciences, Changchun University of Chinese Medicine, Changchun 130117, Jilin, PR China.
authors.forename: Lu
authors.initials: L
authors.lastname: Li
authors.affiliation: College of Basic Medical Sciences, Changchun University of Chinese Medicine, Changchun 130117, Jilin, PR China.
authors.forename: Xingyun
authors.initials: X
authors.lastname: Wang
authors.affiliation: College of Basic Medical Sciences, Changchun University of Chinese Medicine, Changchun 130117, Jilin, PR China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Liu
authors.affiliation: College of Basic Medical Sciences, Changchun University of Chinese Medicine, Changchun 130117, Jilin, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Guo
authors.affiliation: School of Pharmaceutical Sciences, Jilin University, Changchun 130021, Jilin, PR China. Electronic address: wht@jlu.edu.cn.
authors.forename: Haotian
authors.initials: H
authors.lastname: Wang
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000627:therapeutic use*; D002374:Catalase / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D007376:Interleukin-2 / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008113:Liver Neoplasms / Q000188:drug therapy* / Q000378:metabolism*; D008315:Malondialdehyde / Q000378:metabolism; D031101:Marsdenia / Q000737:chemistry*; D051379:Mice; D010936:Plant Extracts / Q000737:chemistry / Q000627:therapeutic use*; D011134:Polysaccharides / Q000737:chemistry* / Q000627:therapeutic use*; D013482:Superoxide Dismutase / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D007376:Interleukin-2; D010936:Plant Extracts; D011134:Polysaccharides; D014409:Tumor Necrosis Factor-alpha; D042461:Vascular Endothelial Growth Factor A; D008315:Malondialdehyde; D002374:Catalase; D013482:Superoxide Dismutase; D005978:Glutathione
keywords: Antioxidant; Antitumor; Hepatic carcinoma; Immunity; Marsdenia tenacissima polysaccharide