title: Study of dendritic cell migration using micro-fabrication.
abstract: Cell migration is a hallmark of dendritic cells (DCs) function. It is needed for DCs to scan their environment in search for antigens as well as to reach lymphatic organs in order to trigger T lymphocyte's activation. Such interaction leads to tolerance in the case of DCs migrating under homeostatic conditions or to immunity in the case of DCs migrating upon encounter with pathogen-associated molecular patterns. Cell migration is therefore essential for DCs to transfer information from peripheral tissues to lymphoid organs, thereby linking innate to adaptive immunity. This stresses the need to unravel the molecular mechanisms involved. However, the tremendous complexity of the tissue microenvironment as well as the limited spatio-temporal resolution of in vivo imaging techniques has made this task difficult. To bypass this problem, we have developed microfabrication-based experimental tools that are compatible with high-resolution imaging. Here, we will discuss how such devices can be used to study DC migration under controlled conditions that mimic their physiological environment in a robust quantitative manner.
authors.affiliation: Institut Curie, CNRS UMR 144, 26 rue d'Ulm, 75005 Paris, France. Electronic address: pablo.vargas@curie.fr.
authors.forename: Pablo
authors.initials: P
authors.lastname: Vargas
authors.affiliation: Institut Curie, Inserm U932, 26 rue d'Ulm, 75005 Paris, France.
authors.forename: Mélanie
authors.initials: M
authors.lastname: Chabaud
authors.affiliation: Institut Curie, CNRS UMR 144, 26 rue d'Ulm, 75005 Paris, France.
authors.forename: Hawa-Racine
authors.initials: HR
authors.lastname: Thiam
authors.affiliation: Institut Curie, Inserm U932, 26 rue d'Ulm, 75005 Paris, France.
authors.forename: Danielle
authors.initials: D
authors.lastname: Lankar
authors.affiliation: Institut Curie, CNRS UMR 144, 26 rue d'Ulm, 75005 Paris, France.
authors.forename: Matthieu
authors.initials: M
authors.lastname: Piel
authors.affiliation: Institut Curie, Inserm U932, 26 rue d'Ulm, 75005 Paris, France.
authors.forename: Ana-Maria
authors.initials: AM
authors.lastname: Lennon-Dumenil
mesh_terms: D056704:Adaptive Immunity; D000818:Animals; D016764:Cell Polarity; D060833:Cellular Microenvironment; D002633:Chemotaxis; D016207:Cytokines / Q000276:immunology / Q000378:metabolism; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism; D004867:Equipment Design; D006801:Humans; D007113:Immunity, Innate; D008853:Microscopy / Q000295:instrumentation / Q000379:methods; D055616:Microtechnology / Q000295:instrumentation / Q000379:methods; D008904:Miniaturization; D010641:Phenotype; D015398:Signal Transduction; D013997:Time Factors
chemical_list: D016207:Cytokines
keywords: Cell migration; Cell polarity; Chemokines; Confinement; Constrictions; Dendritic cells; Gradients; Imaging; Leukocytes; Lymphocytes; Micro-fabrication; Motility; Persistence