title: Inflammation response and cytotoxic effects in human THP-1 cells of size-fractionated PM10 extracts in a polluted urban site.
abstract: To contribute to a greater characterization of the airborne particulate matter's toxicity, size-fractionated PM10 was sampled during different seasons in a polluted urban site in Torino, a northern Italian city. Three main size fractions (PM10 - 3 μm; PM3 - 0.95 μm; PM < 0.95 μm) extracts (organic and aqueous) were assayed with THP-1 cells to evaluate their effects on cell proliferation, LDH activity, TNFα, IL-8 and CYP1A1 expression. The mean PM10 concentrations were statistically different in summer and in winter and the finest fraction PM<0.95 was always higher than the others. Size-fractionated PM10 extracts, sampled in an urban traffic meteorological-chemical station produced size-related toxicological effects in relation to season and particles extraction. The PM summer extracts induced a significant release of LDH compared to winter and produced a size-related effect, with higher values measured with PM10-3. Exposure to size-fractionated PM10 extracts did not induce significant expression of TNFα. IL-8 expression was influenced by exposure to size-fractionated PM10 extracts and statistically significant differences were found between kind of extracts for both seasons. The mean fold increases in CYP1A1 expression were statistically different in summer and in winter; winter fraction extracts produced a size-related effect, in particular for organic samples with higher values measured with PM<0.95 extracts. Our results confirm that the only measure of PM can be misleading for the assessment of air quality moreover we support efforts toward identifying potential effect-based tools (e.g. in vitro test) that could be used in the context of the different monitoring programs.
authors.affiliation: Department of Public Health and Pediatrics, University of Torino, Piazza Polonia, 94, 10126, Torino, Italy. Electronic address: tiziana.schiliro@unito.it.
authors.forename: T
authors.initials: T
authors.lastname: Schilirò
authors.affiliation: Department of Public Health and Pediatrics, University of Torino, Piazza Polonia, 94, 10126, Torino, Italy. Electronic address: luca.al@hotmail.it.
authors.forename: L
authors.initials: L
authors.lastname: Alessandria
authors.affiliation: Department of Public Health and Pediatrics, University of Torino, Piazza Polonia, 94, 10126, Torino, Italy. Electronic address: sara.bonetta@unito.it.
authors.forename: S
authors.initials: S
authors.lastname: Bonetta
authors.affiliation: Department of Public Health and Pediatrics, University of Torino, Piazza Polonia, 94, 10126, Torino, Italy. Electronic address: elisabetta.carraro@unito.it.
authors.forename: E
authors.initials: E
authors.lastname: Carraro
authors.affiliation: Department of Public Health and Pediatrics, University of Torino, Piazza Polonia, 94, 10126, Torino, Italy. Electronic address: giorgio.gilli@unito.it.
authors.forename: G
authors.initials: G
authors.lastname: Gilli
mesh_terms: D000393:Air Pollutants / Q000032:analysis / Q000633:toxicity*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D002947:Cities; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics; D016207:Cytokines / Q000235:genetics / Q000276:immunology; D004784:Environmental Monitoring / Q000379:methods*; D006801:Humans; D016209:Interleukin-8 / Q000235:genetics / Q000276:immunology; D007558:Italy; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D009000:Monocytes / Q000187:drug effects / Q000276:immunology; D010316:Particle Size; D052638:Particulate Matter / Q000032:analysis / Q000633:toxicity*; D012621:Seasons; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000276:immunology; D014507:Urbanization
chemical_list: D000393:Air Pollutants; D016207:Cytokines; D016209:Interleukin-8; D052638:Particulate Matter; D014409:Tumor Necrosis Factor-alpha; D007770:L-Lactate Dehydrogenase; C569463:CYP1A1 protein, human; D019363:Cytochrome P-450 CYP1A1
keywords: CYP1A1; IL-8; LDH; Size-fractionated PM(10); THP-1 cells; TNFα