title: Genome-wide epigenetic cross-talk between DNA methylation and H3K27me3 in zebrafish embryos.
abstract: DNA methylation and histone modifications are epigenetic marks implicated in the complex regulation of vertebrate embryogenesis. The cross-talk between DNA methylation and Polycomb-dependent H3K27me3 histone mark has been reported in a number of organisms [1], [2], [3], [4], [5], [6], [7] and both marks are known to be required for proper developmental progression. Here we provide genome-wide DNA methylation (MethylCap-seq) and H3K27me3 (ChIP-seq) maps for three stages (dome, 24 hpf and 48 hpf) of zebrafish (Danio rerio) embryogenesis, as well as all analytical and methodological details associated with the generation of this dataset. We observe a strong antagonism between the two epigenetic marks present in CpG islands and their compatibility throughout the bulk of the genome, as previously reported in mammalian ESC lines (Brinkman et al., 2012). Next generation sequencing data linked to this project have been deposited in the Gene Expression Omnibus (GEO) database under accession numbers GSE35050 and GSE70847.
authors.affiliation: Centro Andaluz de Biología del Desarrollo, Consejo Superior de Investigaciones Científicas/Universidad Pablo de Olavide, Seville 41013, Spain.
authors.forename: Elisa
authors.initials: E
authors.lastname: de la Calle Mustienes
authors.affiliation: Centro Andaluz de Biología del Desarrollo, Consejo Superior de Investigaciones Científicas/Universidad Pablo de Olavide, Seville 41013, Spain.
authors.forename: Jose Luis
authors.initials: JL
authors.lastname: Gómez-Skarmeta
authors.affiliation: ARC Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley 6009, Australia.
authors.forename: Ozren
authors.initials: O
authors.lastname: Bogdanović
keywords: DNA methylation; Embryogenesis; Polycomb; Zebrafish