title: Expression of Selenoprotein Genes Is Affected by Heat Stress in IPEC-J2 Cells.
abstract: The aim of this study was to explore the impacts of heat stress (HS) on expressions of selenoprotein genes in IPEC-J2 cells. Cells were cultured with 5 % CO2-humidified chamber at 37 °C until the cells grew to complete confluence and then exposed to a mild hyperthermia at 41.5 °C (HS) or 37 °C (control) for another 24 h, finally harvested for total RNA or protein extraction. Real-time quantitative PCRs (qPCRs) were performed to compare gene expression of 25 selenoprotein genes, 3 tight junction-related genes, and 10 inflammation-related genes. Protein expressions of heat shock protein 70 (Hsp70) and selenoprotein X and P (SelX and SelP) were also investigated by Western blot. The results showed that HS up-regulated (P < 0.05) Hsp70 and one tight junction-related gene [zonula occludens-1 (Zo-1)] in IPEC-J2 cells. At the same time, HS up-regulated (P < 0.05) 4 selenoprotein genes (Gpx3, Dio2, Selk, Sels) and three inflammation-related genes (Il-6, Icam-1, Tgf-β) and down-regulated (P < 0.05 or as indicated) six selenoprotein genes (Gpx2, Gpx6, Txnrd1, Selh, Selm, Selx) and three inflammation-related genes (Ifn-β, Mcp-1, Tnf-α) in the cells. HS also exhibited impacts on protein expressions, which up-regulated Hsp70, down-regulated SelX, and showed no effect on SelP in IPEC-J2 cells. Our results showed that HS affected the expression of inflammation-related genes and up-regulated gene and protein expressions of Hsp70. The changes of so many selenoprotein genes expression implied a potential link between selenoprotein genes and HS. Moreover, the results provided by this IPEC-J2 model may be used to further study the interactive mechanisms between selenoprotein function and potential intestinal damage induced by HS.
authors.affiliation: Animal Nutrition Institute, Sichuan Agricultural University, No 211 Huimin Road, Chengdu, Sichuan, 611130, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Cao
authors.affiliation: Animal Nutrition Institute, Sichuan Agricultural University, No 211 Huimin Road, Chengdu, Sichuan, 611130, China.
authors.forename: Jiayong
authors.initials: J
authors.lastname: Tang
authors.affiliation: Sichuan Provincial General Station for Animal Husbandry, Chengdu, 610041, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Li
authors.affiliation: Animal Nutrition Institute, Sichuan Agricultural University, No 211 Huimin Road, Chengdu, Sichuan, 611130, China.
authors.forename: Jingyang
authors.initials: J
authors.lastname: Xu
authors.affiliation: Animal Nutrition Institute, Sichuan Agricultural University, No 211 Huimin Road, Chengdu, Sichuan, 611130, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Jia
authors.affiliation: Animal Nutrition Institute, Sichuan Agricultural University, No 211 Huimin Road, Chengdu, Sichuan, 611130, China.
authors.forename: Guangmang
authors.initials: G
authors.lastname: Liu
authors.affiliation: Animal Nutrition Institute, Sichuan Agricultural University, No 211 Huimin Road, Chengdu, Sichuan, 611130, China.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Chen
authors.affiliation: Animal Nutrition Institute, Sichuan Agricultural University, No 211 Huimin Road, Chengdu, Sichuan, 611130, China.
authors.forename: Haiying
authors.initials: H
authors.lastname: Shang
authors.affiliation: Animal Nutrition Institute, Sichuan Agricultural University, No 211 Huimin Road, Chengdu, Sichuan, 611130, China.
authors.forename: Jingyi
authors.initials: J
authors.lastname: Cai
authors.affiliation: Animal Nutrition Institute, Sichuan Agricultural University, No 211 Huimin Road, Chengdu, Sichuan, 611130, China. zhua666@126.com.
authors.forename: Hua
authors.initials: H
authors.lastname: Zhao
mesh_terms: D000818:Animals; D002460:Cell Line; D006358:Hot Temperature; D051140:Selenoproteins / Q000235:genetics* / Q000378:metabolism; D013552:Swine
chemical_list: D051140:Selenoproteins
keywords: Expression; Heat stress; IPEC-J2; Selenoprotein