title: Purification of Na,K-ATPase from Pig Kidney.
abstract: The method of purification of Na,K-ATPase from pig kidney is based on a differential centrifugation and SDS-treatment of a microsomal preparation. The yield is 0.4 mg protein per 1 g tissue with the specific (ouabain-sensitive) activity of 25-28 μmol Pi/min per mg protein and nucleotide binding capacity of 3 nmol/mg. The protein/lipid ratio is 1/1 (mg/mg) with a protein purity of ~80 %.
authors.affiliation: Department of Biomedicine, Aarhus University, Ole Worms Allé 6, bldg. 1185, DK-8000, Aarhus C, Denmark. nf@biomed.au.dk.|Centre for Membrane Pumps in Cells and Disease-PUMPkin, Danish National Research Foundation, Gustav Wieds Vej 10C, DK-8000, Aarhus C, Denmark. nf@biomed.au.dk.
authors.forename: Natalya U
authors.initials: NU
authors.lastname: Fedosova
mesh_terms: D000818:Animals; D007668:Kidney / Q000737:chemistry / Q000201:enzymology*; D007700:Kinetics; D008967:Molecular Biology / Q000379:methods*; D009711:Nucleotides / Q000737:chemistry; D010042:Ouabain / Q000737:chemistry; D000254:Sodium-Potassium-Exchanging ATPase / Q000737:chemistry / Q000302:isolation & purification*; D013552:Swine
chemical_list: D009711:Nucleotides; D010042:Ouabain; D000254:Sodium-Potassium-Exchanging ATPase
keywords: Kidney; Microsomal fraction differential centrifugation; Na,K-ATPase; SDS -treatment