title: The Roles of MicroRNA-122 Overexpression in Inhibiting Proliferation and Invasion and Stimulating Apoptosis of Human Cholangiocarcinoma Cells.
abstract: Our study investigated whether microRNA-122 (miR-122) played important roles in the proliferation, invasion and apoptosis of human cholangiocarcinoma (CC) cells. QBC939 and RBE cells lines were chosen and divided into five groups: miR-122 mimic group, anti-miR-122 group, negative control (NC) group, mock group and blank group. MiR-122 expression was measured by qRT-PCR. Roles of miR-122 in cell proliferation, apoptosis and invasion were investigated using MTT assay, flow cytometer and Transwell invasion assay, respectively. MiR-122 expression was lower in CC tissues and QBC939 cell than that in normal bile duct tissues, HCCC-9810 and RBE cells. In both QBC939 and RBE cells lines, miR-122 expression was higher in miR-122 mimic group than that in NC group, mock group and blank group; opposite results were found in anti-miR-122 group. Cell proliferation and invasion were remarkably inhibited in miR-122 mimic group after 48 h/72 h transfection, while apoptotic cells numbers were much greater in miR-122 mimic group; the opposite results were obtained from anti-miR-122 group (all P < 0.05). MiR-122 expression was significantly weaker in CC tissues, and miR-122 overexpression might play pivotal roles in inhibiting proliferation, stimulating apoptosis and suppressing invasion of CC cells, suggesting a new target for CC diagnosis and treatment.
authors.affiliation: Department of General Surgery, Hainan Provincial people's Hospital, Haikou 570311, P.R. China.
authors.forename: Ning
authors.initials: N
authors.lastname: Liu
authors.affiliation: Department of Geratology, Hainan Provincial people's Hospital, Haikou 570311, P.R. China.
authors.forename: Fan
authors.initials: F
authors.lastname: Jiang
authors.affiliation: Department of Pancreatic Surgery, Changhai Hospital, Second Military Medical University, Shanghai 200433, P.R. China.
authors.forename: Tian-Lin
authors.initials: TL
authors.lastname: He
authors.affiliation: Department of Interventional, the Third Hospital of PLA, P.R. China.
authors.forename: Jun-Kuan
authors.initials: JK
authors.lastname: Zhang
authors.affiliation: Department of Oncology, Xiangyang Central Hospital, Xiangyang 441021, P.R. China.
authors.forename: Juan
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Department of Oncology, Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430024, P.R. China.
authors.forename: Chun
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Interventional, the Third Hospital of PLA, P.R. China.
authors.forename: Gui-Xing
authors.initials: GX
authors.lastname: Jiang
authors.affiliation: Department of Interventional, the Third Hospital of PLA, P.R. China.
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Cao
authors.affiliation: Department of Interventional, the Third Hospital of PLA, P.R. China.
authors.forename: Peng-Cheng
authors.initials: PC
authors.lastname: Kang
authors.affiliation: Department of Interventional, the Third Hospital of PLA, P.R. China.
authors.forename: Xiang-Yu
authors.initials: XY
authors.lastname: Zhong
authors.affiliation: Department of Interventional, the Third Hospital of PLA, P.R. China.
authors.forename: Tian-Yu
authors.initials: TY
authors.lastname: Lin
authors.affiliation: Department of Interventional, the Third Hospital of PLA, P.R. China.
authors.forename: Yun-Fu
authors.initials: YF
authors.lastname: Cui
mesh_terms: D017209:Apoptosis / Q000235:genetics; D001652:Bile Ducts / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics*; D018281:Cholangiocarcinoma / Q000235:genetics* / Q000473:pathology; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D009361:Neoplasm Invasiveness / Q000235:genetics*
chemical_list: C531907:MIRN122 microRNA, human; D035683:MicroRNAs