title: Temporal Evolution of MRI Characteristics in Dogs with Collagenase-Induced Intracerebral Hemorrhage.
abstract: Intracerebral hemorrhage (ICH) is one of the most lethal types of stroke. Neuroimaging techniques, particularly MRI, have improved the diagnostic accuracy of ICH. The MRI characteristics of the evolving stages of ICH in humans-but not those in dogs-have been described. In this study, we document the temporal MRI characteristics in a canine model of collagenase-induced ICH. Specifically, ICH was induced in 5 healthy beagles by injecting 500 U of bacterial collagenase from Clostridium histolyticum, which was delivered into the parietal lobe over 5 min by using a microinfusion pump. T1- and T2-weighted, fluid-attenuated inversion recovery, gradient-echo (GRE), and diffusion-weighted (DWI) imaging and measurement of the apparent diffusion coefficient (ADC) were performed serially at 6 different time points (before and 12 h, 3 d, 5 d, 10 d and 24 d after hemorrhage) by using a 3-T MR system. The temporal changes of T1 signal intensity (SI) corresponded well with the reported human data. The temporal changes of T2 and GRE sequences, with the exception of T2 and GRE hyperintensities at the early subacute stage, also matched. ADC measurements were high at the early subacute stage, and DWI-SI positively correlated with T2- and GRE-SI from the early subacute stage onward. In conclusion, MRI is an ideal method for characterizing the temporal evolution of parenchymal alterations after ICH in dogs. These data might be useful for differentiating clinical stages of ICH in dogs.
authors.affiliation: Laboratory of Molecular Imaging and Translational Research, Veterinary Medical Center, College of Veterinary Medicine, Chungbuk National University, Cheongju, South Korea.
authors.forename: Daegi
authors.initials: D
authors.lastname: An
authors.affiliation: Division of Convergence Biotechnology, Korea Basic Science Institute, Ochang, South Korea.
authors.forename: Junyong
authors.initials: J
authors.lastname: Park
authors.affiliation: Department of Veterinary Pathology, Small Animal Tumor Diagnostic Center, College of Veterinary Medicine, Konkuk University, Seoul, South Korea.
authors.forename: Jong-Il
authors.initials: JI
authors.lastname: Shin
authors.affiliation: Department of Biomedical Engineering, Kyung Hee University, Yongin, South Korea.
authors.forename: Hyung-Joong
authors.initials: HJ
authors.lastname: Kim
authors.affiliation: Research Institute of Life Sciences, Gyeongsang National University, Jinju, South Korea.
authors.forename: Dong-In
authors.initials: DI
authors.lastname: Jung
authors.affiliation: Veterinary Medical Center, College of Veterinary Medicine, Chungbuk National University, Cheongju, South Korea.
authors.forename: Ji-Houn
authors.initials: JH
authors.lastname: Kang
authors.affiliation: Veterinary Medical Center, College of Veterinary Medicine, Chungbuk National University, Cheongju, South Korea.
authors.forename: Gonhyung
authors.initials: G
authors.lastname: Kim
authors.affiliation: Veterinary Medical Center, College of Veterinary Medicine, Chungbuk National University, Cheongju, South Korea.
authors.forename: Dong-Woo
authors.initials: DW
authors.lastname: Chang
authors.affiliation: Department of Veterinary Pathology, Small Animal Tumor Diagnostic Center, College of Veterinary Medicine, Konkuk University, Seoul, South Korea.
authors.forename: Jung-Hyang
authors.initials: JH
authors.lastname: Sur
authors.affiliation: Veterinary Medical Center, College of Veterinary Medicine, Chungbuk National University, Cheongju, South Korea.
authors.forename: Mhan-Pyo
authors.initials: MP
authors.lastname: Yang
authors.affiliation: Division of Convergence Biotechnology, Korea Basic Science Institute, Ochang, South Korea.
authors.forename: Chulhyun
authors.initials: C
authors.lastname: Lee
authors.affiliation: Laboratory of Molecular Imaging and Translational Research, Veterinary Medical Center, College of Veterinary Medicine, Chungbuk National University, Cheongju, South Korea. kangbt@chungbuk.ac.kr.
authors.forename: Byeong-Teck
authors.initials: BT
authors.lastname: Kang
mesh_terms: D000818:Animals; D002543:Cerebral Hemorrhage / Q000139:chemically induced / Q000175:diagnosis / Q000601:surgery / Q000662:veterinary*; D017364:Collagenases / Q000633:toxicity*; D004283:Dog Diseases / Q000139:chemically induced / Q000175:diagnosis* / Q000601:surgery; D004285:Dogs; D005260:Female; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male
chemical_list: D017364:Collagenases