title: Anti-Ulcerogenic Properties of Lycium chinense Mill Extracts against Ethanol-Induced Acute Gastric Lesion in Animal Models and Its Active Constituents.
abstract: The objective of this study was to explore the gastroprotective properties of the aerial part of Lycium chinense Mill (LCA) against ethanol-induced gastric mucosa lesions in mice models. Administration of LCA at doses of 50, 100, 200 and 400 mg/kg body weight prior to ethanol consumption dose dependently inhibited gastric ulcers. The gastric mucosal injury was analyzed by gastric juice acidity, glutathione (GSH), superoxide dismutase (SOD), malondialdehyde (MDA), myeloperoxidase (MPO) activities. Furthermore, the levels of the inflammatory mediators, tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β) in serum were also analyzed using ELISA. Pathological changes were also observed with the aid of hematoxylin-eosin (HE) staining. Our results indicated that LCA significantly reduced the levels of MPO, MDA and increased SOD and GSH activities. Furthermore, LCA also significantly inhibited the levels of TNF-α, IL-6, and IL-1β in the serum of ulcerated mice in a dose dependent manner. Immunohistological analysis indicated that LCA also significantly attenuated the overexpression of nuclear factor-κB in pretreated mice models. This findings suggests Lycium chinense Mill possesses gastroprotective properties against ethanol-induced gastric injury and could be a possible therapeutic intervention in the treatment and management of gastric ulcers.
authors.affiliation: Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, China. pere@fastermail.com.|School of Pharmacy, Jiangsu University, Zhenjiang 212013, China. pere@fastermail.com.
authors.forename: Opeyemi J
authors.initials: OJ
authors.lastname: Olatunji
authors.affiliation: School of Pharmacy, Jiangsu University, Zhenjiang 212013, China. chhx2011@ujs.edu.cn.
authors.forename: Hongxia
authors.initials: H
authors.lastname: Chen
authors.affiliation: Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, China. njgzhou@163.com.|Dongtai Institute of Tidal Flats, Nanjing Branch of Chinese Academy of Sciences, Dongtai 224200, China. njgzhou@163.com.
authors.forename: Yifeng
authors.initials: Y
authors.lastname: Zhou
mesh_terms: D000818:Animals; D000897:Anti-Ulcer Agents / Q000494:pharmacology / Q000627:therapeutic use*; D016207:Cytokines / Q000097:blood; D004195:Disease Models, Animal; D004353:Drug Evaluation, Preclinical; D000431:Ethanol; D005750:Gastric Juice / Q000378:metabolism; D005978:Glutathione / Q000097:blood; D006863:Hydrogen-Ion Concentration; D032305:Lycium / Q000737:chemistry*; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008813:Mice, Inbred ICR; D018384:Oxidative Stress; D009195:Peroxidase / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D013270:Stomach / Q000187:drug effects / Q000473:pathology; D013276:Stomach Ulcer / Q000097:blood / Q000139:chemically induced / Q000188:drug therapy*; D013482:Superoxide Dismutase / Q000097:blood; D051996:Transcription Factor RelA / Q000378:metabolism
chemical_list: D000897:Anti-Ulcer Agents; D016207:Cytokines; D010936:Plant Extracts; C498144:Rela protein, mouse; D051996:Transcription Factor RelA; D000431:Ethanol; D008315:Malondialdehyde; D009195:Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione
keywords: Lycium chinense Mill; ethanol; gastric ulcer; gastroprotective