title: Influence of boar breeds or hybrid genetic composition on semen quality and seminal plasma biochemical variables.
abstract: The enzyme concentrations of seminal plasma are important for spermatozoa metabolism and function in boars. The need has arisen for introducing a biochemical evaluation of semen, along with the usual standard semen analyses. There are no data on the influence of boar breeds on the seminal plasma biochemical variables investigated in this study. Therefore, the objective was to determine the influence of breed and hybrid genetic composition of boars on semen quality and seminal plasma biochemical variables. Semen samples of 27 boars (Swedish Landrace, German Landrace, Large White, Pietrain and Pig Improvement Company hybrid-PIC-hybrid), aged between 1.5 and 3 years, were collected. After evaluation of semen quality, the seminal plasma was separated from the spermatozoa by centrifugation of semen. The seminal plasma was subjected to spectrophotometric analysis to determine alkaline phosphatase (ALP), acid phosphatase (ACP), γ-glutamyltransferase (GGT), creatine kinase (CK) and lactate dehydrogenase (LDH) and to atomic absorption spectrophotometric analysis to measure the concentration of calcium and magnesium. Conventional semen quality variables differed depending on breed and PIC-hybrid genetic composition, though these differences were typically insignificant. In the seminal plasma, significant differences were determined in enzyme activity (ALP, GGT, CK and LDH) and in calcium concentration among boars of different breeds. There are, therefore, differences in semen quality and significant differences in the seminal plasma biochemical variables among boars of different breeds and PIC-hybrid genetic composition. The data and differences in semen variables detected in the present study provide knowledge for enhancing evaluation and monitoring of boar reproductive potential, semen quality and explain the potential causes of boar infertility.
authors.affiliation: Department of Physiology and Radiobiology, Faculty of Veterinary Medicine University of Zagreb, Zagreb, Croatia.
authors.forename: Ivona Žura
authors.initials: IŽ
authors.lastname: Žaja
authors.affiliation: Clinic for Obstetrics and Reproduction, Faculty of Veterinary Medicine University of Zagreb, Zagreb, Croatia. Electronic address: smarko@vef.hr.
authors.forename: Marko
authors.initials: M
authors.lastname: Samardžija
authors.affiliation: Clinic for Obstetrics and Reproduction, Faculty of Veterinary Medicine University of Zagreb, Zagreb, Croatia.
authors.forename: Silvijo
authors.initials: S
authors.lastname: Vince
authors.affiliation: Center for Reproduction and Animal Breeding of Croatia d.o.o., Zagreb, Croatia.
authors.forename: Ivanka
authors.initials: I
authors.lastname: Majić-Balić
authors.affiliation: Department of Physiology and Radiobiology, Faculty of Veterinary Medicine University of Zagreb, Zagreb, Croatia.
authors.forename: Marinko
authors.initials: M
authors.lastname: Vilić
authors.affiliation: Veterinary Practice Đurđevac, Đurđevac, Croatia.
authors.forename: Dražen
authors.initials: D
authors.lastname: Đuričić
authors.affiliation: Department of Physiology and Radiobiology, Faculty of Veterinary Medicine University of Zagreb, Zagreb, Croatia.
authors.forename: Suzana
authors.initials: S
authors.lastname: Milinković-Tur
mesh_terms: D000818:Animals; D006824:Hybridization, Genetic; D008297:Male; D012661:Semen / Q000737:chemistry* / Q000502:physiology; D055101:Semen Analysis / Q000662:veterinary*; D013552:Swine / Q000235:genetics* / Q000502:physiology
keywords: Biochemical indicators; Boar; Breed; Ejaculate quality; Seminal plasma