title: Global miRNA Expression Profiling Identifies miR-1290 as Novel Potential oncomiR in Laryngeal Carcinoma.
abstract: BACKGROUND
  Laryngeal squamous cell carcinoma (LSCC) is the most common group among head and neck cancers. LSCC is characterized by a high incidence in Europe. With the aim of better understanding its genetic background we performed global miRNA expression profiling of LSCC cell lines and primary specimens. By this approach we identified a cohort of 33 upregulated and 9 downregulated miRNA genes in LSCC as compared to epithelial no tumor controls.
  
  
  RESULTS
  Within this group we identified overexpression of the novel miR-1290 gene not reported in the context of LSCC before. Using a combined bioinformatical approach in connection with functional analysis we delineated two putative target genes of miR-1290 namely ITPR2 and MAF which are significantly downregulated in LSCC. They are interesting candidates for tumor suppressor genes as they are implicated in apoptosis and other processes deregulated in cancer.
  
  
  CONCLUSIONS
  Taken together, we propose miR-1290 as the new oncomiR involved in LSCC pathogenesis. Additionally, we suggest that the oncogenic potential of miR-1290 might be expressed by the involvement in downregulation of its target genes MAF and ITPR2.
authors.affiliation: Institute of Human Genetics, Polish Academy of Sciences, Department of Cancer Genetics, Poznan, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Janiszewska
authors.affiliation: Institute of Human Genetics, Polish Academy of Sciences, Department of Cancer Genetics, Poznan, Poland.
authors.forename: Marcin
authors.initials: M
authors.lastname: Szaumkessel
authors.affiliation: Institute of Human Genetics, Polish Academy of Sciences, Department of Cancer Genetics, Poznan, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Kostrzewska-Poczekaj
authors.affiliation: Institute of Human Genetics, Polish Academy of Sciences, Department of Cancer Genetics, Poznan, Poland.
authors.forename: Kinga
authors.initials: K
authors.lastname: Bednarek
authors.affiliation: Institute of Human Genetics, Polish Academy of Sciences, Department of Cancer Genetics, Poznan, Poland.
authors.forename: Julia
authors.initials: J
authors.lastname: Paczkowska
authors.affiliation: Department of Otolaryngology and Laryngological Oncology, University of Medical Sciences, Poznan, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Jackowska
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery and Department of Medical Biochemistry, Turku University Hospital and University of Turku, Turku, Finland.
authors.forename: Reidar
authors.initials: R
authors.lastname: Grenman
authors.affiliation: Institute of Human Genetics, Polish Academy of Sciences, Department of Cancer Genetics, Poznan, Poland.|Department of Audiology and Phoniatry, University of Medical Sciences, Poznan, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Szyfter
authors.affiliation: Department of Otolaryngology and Laryngological Oncology, University of Medical Sciences, Poznan, Poland.
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Wierzbicka
authors.affiliation: Institute of Human Genetics, Polish Academy of Sciences, Department of Cancer Genetics, Poznan, Poland.|Department of Otolaryngology and Laryngological Oncology, University of Medical Sciences, Poznan, Poland.
authors.forename: Maciej
authors.initials: M
authors.lastname: Giefing
authors.affiliation: Institute of Human Genetics, Polish Academy of Sciences, Department of Cancer Genetics, Poznan, Poland.|Department of Hematology, K. Marcinkowski University of Medical Sciences, Poznan, Poland.
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Jarmuz-Szymczak
mesh_terms: D020413:3' Untranslated Regions; D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D045744:Cell Line, Tumor; D019295:Computational Biology / Q000379:methods; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053496:Inositol 1,4,5-Trisphosphate Receptors / Q000235:genetics*; D007822:Laryngeal Neoplasms / Q000235:genetics*; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D051257:Proto-Oncogene Proteins c-maf / Q000235:genetics*
chemical_list: D020413:3' Untranslated Regions; C506927:ITPR2 protein, human; D053496:Inositol 1,4,5-Trisphosphate Receptors; C495607:MAF protein, human; C574604:MIRN1290 microRNA, human; D035683:MicroRNAs; D051257:Proto-Oncogene Proteins c-maf