title: Zingerone ameliorates lipopolysaccharide-induced acute kidney injury by inhibiting Toll-like receptor 4 signaling pathway.
abstract: Acute kidney injury (AKI) is a serious complication of sepsis. Zingerone, a phenolic alkanone isolated from ginger, has been reported to have anti-inflammatory effect. The aim of this study was to investigate the therapeutic effects of zingerone on lipopolysaccharide (LPS)-induced AKI in mice. Zingerone was administrated 1h after LPS challenge. The production of blood urea nitrogen (BUN) and creatinine were measured in this study. The expressions of inflammatory cytokines in serum and kidney tissues were detected by ELISA. The expressions of Toll-like receptor 4 (TLR4), MyD88, TRIF, Nuclear factor Kappa B (NF-κB) and IκB were measured by Western blotting. The results showed that zingerone suppressed LPS-induced BUN, creatinine, and inflammatory cytokines TNF-α, IL-6 and IL-1β levels in a dose-dependent manner. Zingerone also attenuated LPS-induced kidney histopathologic changes. Furthermore, zingerone was found to inhibit LPS-induced TLR4, MyD88, TRIF expression and NF-κB activation. In conclusion, the current study demonstrated that zingerone inhibited LPS-induced AKI by suppressing TLR4/NF-κB signaling pathway.
authors.affiliation: Tianjin Medical University, Graduate School, China; Department of Nephrology, Affiliated Hospital of Logistic University of Chinese People's Armed Police Force (PAPF), China.
authors.forename: Jie
authors.initials: J
authors.lastname: Song
authors.affiliation: Institute for Disaster & Emergency Rescue Medicine, Affiliated Hospital of Logistic University of Chinese People's Armed Police Force (PAPF), China.
authors.forename: Hao-jun
authors.initials: HJ
authors.lastname: Fan
authors.affiliation: Department of Nephrology, Affiliated Hospital of Logistic University of Chinese People's Armed Police Force (PAPF), China.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.affiliation: Institute for Disaster & Emergency Rescue Medicine, Affiliated Hospital of Logistic University of Chinese People's Armed Police Force (PAPF), China.
authors.forename: Hui
authors.initials: H
authors.lastname: Ding
authors.affiliation: Institute for Disaster & Emergency Rescue Medicine, Affiliated Hospital of Logistic University of Chinese People's Armed Police Force (PAPF), China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Lv
authors.affiliation: Institute for Disaster & Emergency Rescue Medicine, Affiliated Hospital of Logistic University of Chinese People's Armed Police Force (PAPF), China. Electronic address: houshikegz36@163.com.
authors.forename: Shi-ke
authors.initials: SK
authors.lastname: Hou
mesh_terms: D058186:Acute Kidney Injury / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology*; D033942:Adaptor Proteins, Vesicular Transport / Q000378:metabolism; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology* / Q000627:therapeutic use; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D006139:Guaiacol / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use; D053583:Interleukin-1beta / Q000096:biosynthesis; D015850:Interleukin-6 / Q000096:biosynthesis; D007668:Kidney / Q000187:drug effects / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D008070:Lipopolysaccharides / Q000494:pharmacology*; D051379:Mice; D008810:Mice, Inbred C57BL; D053594:Myeloid Differentiation Factor 88 / Q000378:metabolism; D051267:NF-E2-Related Factor 2 / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects*; D051197:Toll-Like Receptor 4 / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
chemical_list: D033942:Adaptor Proteins, Vesicular Transport; D000894:Anti-Inflammatory Agents, Non-Steroidal; D006139:Guaiacol; D053583:Interleukin-1beta; D015850:Interleukin-6; D008070:Lipopolysaccharides; D053594:Myeloid Differentiation Factor 88; D051267:NF-E2-Related Factor 2; D016328:NF-kappa B; D051197:Toll-Like Receptor 4; D014409:Tumor Necrosis Factor-alpha; C495636:Nfe2l2 protein, mouse; C476286:Ticam1 protein, mouse; C013738:zingerone
keywords: Acute kidney injury; LPS; NF-κB; TLR4; Zingerone