title: Crosslinked hydrogels based on biological macromolecules with potential use in skin tissue engineering.
abstract: Zero-length crosslinked hydrogels have been synthesized by covalent linking of three natural polymers (collagen, hyaluronic acid and sericin), in the presence of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide. The hydrogels have been investigated by FT-IR spectroscopy, microcalorimetry, in vitro swelling, enzymatic degradation, and in vitro cell viability studies. The obtained crosslinked hydrogels showed a macroporous structure, high swelling degree and in vitro enzymatic resistance compared to uncrosslinked collagen. The in vitro cell viability studies performed on normal human dermal fibroblasts assessed the sericin proliferation properties indicating a potential use of the hydrogels based on collagen, hyaluronic acid and sericin in skin tissue engineering.
authors.affiliation: "Gheorghe Asachi" Technical University, Faculty of Chemical Engineering and Environmental Protection, Department of Natural and Synthetic Polymers, 73 Prof. dr. docent Dimitrie Mangeron Street, 700050 Iasi, Romania; Université de Rouen, Laboratoire Polymères Biopolymères Surfaces, F-76821 Mont Saint Aignan, France.
authors.forename: Raluca
authors.initials: R
authors.lastname: Vulpe
authors.affiliation: "Gheorghe Asachi" Technical University, Faculty of Chemical Engineering and Environmental Protection, Department of Natural and Synthetic Polymers, 73 Prof. dr. docent Dimitrie Mangeron Street, 700050 Iasi, Romania; Academy of Romanian Scientist, 54 Splaiul Indenpendentei Street, 050094 Bucuresti, Romania. Electronic address: marpopa@tuiasi.ro.
authors.forename: Marcel
authors.initials: M
authors.lastname: Popa
authors.affiliation: Normandie Université, France; Université de Rouen, Laboratoire Polymères Biopolymères Surfaces, F-76821 Mont Saint Aignan, France; CNRS UMR 6270 & FR3038, F-76821 Mont Saint Aignan, France.
authors.forename: Luc
authors.initials: L
authors.lastname: Picton
authors.affiliation: "Grigore T. Popa" University of Medicine and Pharmacy, Faculty of Medical Bioengineering, Department of Biological Sciences, 9-13 Kogalniceanu Street, 700454 Iasi, Romania.
authors.forename: Vera
authors.initials: V
authors.lastname: Balan
authors.affiliation: Normandie Université, France; Université de Rouen, Laboratoire Polymères Biopolymères Surfaces, F-76821 Mont Saint Aignan, France; CNRS UMR 6270 & FR3038, F-76821 Mont Saint Aignan, France.
authors.forename: Virginie
authors.initials: V
authors.lastname: Dulong
authors.affiliation: "Grigore T. Popa" University of Medicine and Pharmacy, Faculty of Medical Bioengineering, Department of Biological Sciences, 9-13 Kogalniceanu Street, 700454 Iasi, Romania.
authors.forename: Maria
authors.initials: M
authors.lastname: Butnaru
authors.affiliation: "Grigore T. Popa" University of Medicine and Pharmacy, Faculty of Medical Bioengineering, Department of Biological Sciences, 9-13 Kogalniceanu Street, 700454 Iasi, Romania.
authors.forename: Liliana
authors.initials: L
authors.lastname: Verestiuc
mesh_terms: D000327:Adsorption; D001672:Biocompatible Materials / Q000737:chemistry*; D003094:Collagen / Q000737:chemistry; D003432:Cross-Linking Reagents / Q000737:chemistry; D048091:Guided Tissue Regeneration; D006820:Hyaluronic Acid / Q000737:chemistry; D020100:Hydrogels / Q000737:chemistry*; D047030:Sericins / Q000737:chemistry; D012867:Skin; D017550:Spectroscopy, Fourier Transform Infrared; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D020100:Hydrogels; D047030:Sericins; D006820:Hyaluronic Acid; D003094:Collagen
keywords: Biocompatibility; Collagen; Hyaluronic acid; Hydrogels; Sericin