title: Effect of iron oxide nanoparticles on the permeability properties of Sf21 cells.
abstract: It was recently reported that nanoparticles could significantly modulate the thermal properties of solutions at subzero temperatures, and as a result, nanoparticles have been widely used in both cryopreservation and cryosurgery. In cryopreservation, the water permeability coefficient of cell membrane is an essential parameter for quantitative investigation of cell dehydration and intracellular ice formation. However, few studies were focused on the effects of nanoparticles on the permeability properties of cell membrane. In order to optimize the processes of cryopreservation with nanoparticles, we measured the permeability properties of Sf21 cells in the presence of iron oxide nanoparticles in this study. The responses of Sf21 cells with iron oxide nanoparticles were obtained by the microperfusion system at -2, 5, 15 and 25 °C, respectively. The osmotically inactive cell volume (Vb), the cell membrane hydraulic conductivity (Lp) and it's activation energy (ELp), and the reference value of Lp at the reference temperature (Lpg) with 0.02%, 0.1% and 0.5% (w/w) iron oxide nanoparticles were determined by 2-parameter (2-p) model at -2, 5, 15 and 25 °C. We analyzed the effects of iron oxide nanoparticles on the permeability properties of the Sf21 cells. The results indicated that iron oxide nanoparticles have a significant influence on membrane permeability properties (Lpg and ELp) of Sf21 cells. The introduction of iron oxide nanoparticles tends to increase the values of Vb and Lpg, while decrease the value of ELp. These findings may provide a new route to optimize the biomaterial cryopreservation.
authors.affiliation: Center for Biomedical Engineering, Department of Electronic Science and Technology, University of Science and Technology of China, Hefei 230027, China.
authors.forename: Jianye
authors.initials: J
authors.lastname: Wang
authors.affiliation: Center for Biomedical Engineering, Department of Electronic Science and Technology, University of Science and Technology of China, Hefei 230027, China; Anhui Provincial Engineering Technology Research Center for Biopreservation and Artificial Organs, Hefei 230027, China. Electronic address: zhaog@ustc.edu.cn.
authors.forename: Gang
authors.initials: G
authors.lastname: Zhao
authors.affiliation: Department of Mechanical Engineering, University of Washington, Seattle, WA 98195, USA.
authors.forename: Zhiquan
authors.initials: Z
authors.lastname: Shu
authors.affiliation: Anhui Provincial Engineering Technology Research Center for Biopreservation and Artificial Organs, Hefei 230027, China; Center for Reproductive Medicine, The Affiliated Hospital of Anhui Medical University, Hefei 230032, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Zhou
authors.affiliation: Anhui Provincial Engineering Technology Research Center for Biopreservation and Artificial Organs, Hefei 230027, China; Center for Reproductive Medicine, The Affiliated Hospital of Anhui Medical University, Hefei 230032, China.
authors.forename: Yunxia
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Center for Biomedical Engineering, Department of Electronic Science and Technology, University of Science and Technology of China, Hefei 230027, China; Department of Mechanical Engineering, University of Washington, Seattle, WA 98195, USA.
authors.forename: Dayong
authors.initials: D
authors.lastname: Gao
mesh_terms: D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism*; D002463:Cell Membrane Permeability / Q000187:drug effects*; D048429:Cell Size; D003080:Cold Temperature; D015925:Cryopreservation / Q000379:methods*; D005290:Ferric Compounds / Q000494:pharmacology*; D053768:Metal Nanoparticles; D046210:Microfluidic Analytical Techniques; D009995:Osmosis; D061987:Sf9 Cells; D018411:Spodoptera / Q000166:cytology; D014867:Water / Q000378:metabolism
chemical_list: D005290:Ferric Compounds; D014867:Water; C000499:ferric oxide
keywords: Cryopreservation; Iron oxide nanoparticles; Microperfusion system; Permeability properties; Sf21 cell