title: Genome-wide analysis of microRNAs identifies the lipid metabolism pathway to be a defining factor in adipose tissue from different sheep.
abstract: MicroRNAs are short (17-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. In recent years, deep sequencing of the transcriptome is increasingly being utilized with the promise of higher sensitivity for the identification of differential expression patterns as well as the opportunity to discover new transcripts, including new alternative isoforms and miRNAs. Here, we utilized RNA-seq technology to perform a genome-wide analysis of miRNAs from the adipose tissue of the two species of sheep to look for clues that might explain the fat deposition differences between the sheep. The RNA-seq analysis detected 3132 miRNAs from the adipose tissue of the Small-tail Han and Dorset sheep, of which 2893 were defined as potential new miRNAs. In addition, 54 miRNAs were differentially expressed between the two breeds of sheep. Gene ontology and pathway analyses of the predicted target genes that negatively associated with the differentially expressed miRNAs revealed that there was less active lipid metabolism in the adipose tissue of Small Tail Han sheep. This study can help understand the underlying mechanisms responsible for the morphological differences related to fat deposition between two breeds of sheep.
authors.affiliation: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Miao
authors.affiliation: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
authors.forename: Qingmiao
authors.initials: Q
authors.lastname: Luo
authors.affiliation: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Qin
authors.affiliation: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
authors.forename: Yuntao
authors.initials: Y
authors.lastname: Guo
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D015536:Down-Regulation / Q000235:genetics; D020869:Gene Expression Profiling; D015723:Gene Library; D063990:Gene Ontology; D016678:Genome; D055106:Genome-Wide Association Study; D050356:Lipid Metabolism / Q000235:genetics*; D053858:Metabolic Networks and Pathways / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D058977:Molecular Sequence Annotation; D015203:Reproducibility of Results; D017422:Sequence Analysis, DNA; D034561:Sheep, Domestic / Q000235:genetics*
chemical_list: D035683:MicroRNAs