title: TBX3 Knockdown Decreases Reprogramming Efficiency of Human Cells.
abstract: TBX3 is a member of the T-box transcription factor family and is involved in the core pluripotency network. Despite this role in the pluripotency network, its contribution to the reprogramming process during the generation of human induced pluripotent stem cells remains elusive. In this respect, we performed reprogramming experiments applying TBX3 knockdown in human fibroblasts and keratinocytes. Knockdown of TBX3 in both somatic cell types decreased the reprogramming efficiencies in comparison to control cells but with unchanged reprogramming kinetics. The resulting iPSCs were indistinguishable from control cells and displayed a normal in vitro differentiation capacity by generating cells of all three germ layers comparable to the controls.
authors.affiliation: Institute of Neuroanatomy, Eberhard Karls University Tübingen, 72074 Tübingen, Germany.
authors.forename: Moritz
authors.identifier: 0000-0002-1476-4939
authors.initials: M
authors.lastname: Klingenstein
authors.affiliation: Institute of Neuroanatomy, Eberhard Karls University Tübingen, 72074 Tübingen, Germany.
authors.forename: Stefanie
authors.identifier: 0000-0001-9682-0211
authors.initials: S
authors.lastname: Raab
authors.affiliation: Institute of Neuroanatomy, Eberhard Karls University Tübingen, 72074 Tübingen, Germany.
authors.forename: Kevin
authors.initials: K
authors.lastname: Achberger
authors.affiliation: Department of Internal Medicine I, Ulm University, 89081 Ulm, Germany.
authors.forename: Alexander
authors.initials: A
authors.lastname: Kleger
authors.affiliation: Institute of Neuroanatomy, Eberhard Karls University Tübingen, 72074 Tübingen, Germany.
authors.forename: Stefan
authors.identifier: 0000-0003-0721-8187
authors.initials: S
authors.lastname: Liebau
authors.affiliation: Institute of Neuroanatomy, Eberhard Karls University Tübingen, 72074 Tübingen, Germany.
authors.forename: Leonhard
authors.identifier: 0000-0002-5439-8101
authors.initials: L
authors.lastname: Linta