title: Different zinc sensitivity of Brassica organs is accompanied by distinct responses in protein nitration level and pattern.
abstract: Zinc is an essential microelement, but its excess exerts toxic effects in plants. Heavy metal stress can alter the metabolism of reactive oxygen (ROS) and nitrogen species (RNS) leading to oxidative and nitrosative damages; although the participation of these processes in Zn toxicity and tolerance is not yet known. Therefore this study aimed to evaluate the zinc tolerance of Brassica organs and the putative correspondence of it with protein nitration as a relevant marker for nitrosative stress. Both examined Brassica species (B. juncea and B. napus) proved to be moderate Zn accumulators; however B. napus accumulated more from this metal in its organs. The zinc-induced damages (growth diminution, altered morphology, necrosis, chlorosis, and the decrease of photosynthetic activity) were slighter in the shoot system of B. napus than in B. juncea. The relative zinc tolerance of B. napus shoot was accompanied by moderate changes of the nitration pattern. In contrast, the root system of B. napus suffered more severe damages (growth reduction, altered morphology, viability loss) and slighter increase in nitration level compared to B. juncea. Based on these, the organs of Brassica species reacted differentially to excess zinc, since in the shoot system modification of the nitration pattern occurred (with newly appeared nitrated protein bands), while in the roots, a general increment in the nitroproteome could be observed (the intensification of the same protein bands being present in the control samples). It can be assumed that the significant alteration of nitration pattern is coupled with enhanced zinc sensitivity of the Brassica shoot system and the general intensification of protein nitration in the roots is attached to relative zinc endurance.
authors.affiliation: Department of Plant Biology, Faculty of Science and Informatics, University of Szeged, Szeged - 6726 Közép fasor 52, Hungary.
authors.forename: Gábor
authors.initials: G
authors.lastname: Feigl
authors.affiliation: Department of Plant Biology, Faculty of Science and Informatics, University of Szeged, Szeged - 6726 Közép fasor 52, Hungary. Electronic address: kolzsu@bio.u-szeged.hu.
authors.forename: Zsuzsanna
authors.initials: Z
authors.lastname: Kolbert
authors.affiliation: Department of Plant Biology, Faculty of Science and Informatics, University of Szeged, Szeged - 6726 Közép fasor 52, Hungary.
authors.forename: Nóra
authors.initials: N
authors.lastname: Lehotai
authors.affiliation: Department of Plant Biology, Faculty of Science and Informatics, University of Szeged, Szeged - 6726 Közép fasor 52, Hungary.
authors.forename: Árpád
authors.initials: Á
authors.lastname: Molnár
authors.affiliation: Department of Plant Biology, Faculty of Science and Informatics, University of Szeged, Szeged - 6726 Közép fasor 52, Hungary.
authors.forename: Attila
authors.initials: A
authors.lastname: Ördög
authors.affiliation: Department of Plant Biology, Faculty of Science and Informatics, University of Szeged, Szeged - 6726 Közép fasor 52, Hungary.
authors.forename: Ádám
authors.initials: Á
authors.lastname: Bordé
authors.affiliation: Department of Plant Biology, Faculty of Science and Informatics, University of Szeged, Szeged - 6726 Közép fasor 52, Hungary.
authors.forename: Gábor
authors.initials: G
authors.lastname: Laskay
authors.affiliation: Department of Plant Biology, Faculty of Science and Informatics, University of Szeged, Szeged - 6726 Közép fasor 52, Hungary.
authors.forename: László
authors.initials: L
authors.lastname: Erdei
mesh_terms: D001937:Brassica / Q000737:chemistry*; D015227:Lipid Peroxidation / Q000187:drug effects; D010788:Photosynthesis / Q000187:drug effects; D018515:Plant Leaves / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry*; D026361:Reactive Nitrogen Species / Q000737:chemistry; D017382:Reactive Oxygen Species / Q000737:chemistry; D015032:Zinc / Q000737:chemistry*
chemical_list: D010940:Plant Proteins; D026361:Reactive Nitrogen Species; D017382:Reactive Oxygen Species; D015032:Zinc
keywords: Brassica juncea; Brassica napus; Protein tyrosine nitration; Reactive nitrogen species; Reactive oxygen species; Zinc tolerance