title: Living cell imaging and Rac1-GTP levels of CXCL12-treated migrating neural progenitor cells in stripe assay.
abstract: This data article contains three figures and three videos related to the research article entitled "Applications of Stripe Assay in the Study of CXCL12-mediated Neural Progenitor Cell Migration and Polarization" Zhang et al. (2015) [1], which uses stripe assay to study mouse neural progenitor cell (NPC) migration and polarization. The current article describes the neurosphere method used to culture NPCs. NPCs in neurospheres and monolayer were characterized using immunocytochemistry method with antibodies against two classic NPC markers: nestin and SOX2. The article also describes method to obtain sufficient protein lysates from NPCs in the stripe assay. When protein lysates were subjected to Rac1 affinity precipitation, Rac1-GTP was detected in the pull-down samples. In addition, the articles provides live cell imaging data to better understand CXCL12-mediated cellular migration and polarization.
authors.affiliation: Center for Translational Neurodegeneration and Regenerative Therapy, Shanghai Tenth People's Hospital affiliated to Tongji University School of Medicine, Shanghai, China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Center for Translational Neurodegeneration and Regenerative Therapy, Shanghai Tenth People's Hospital affiliated to Tongji University School of Medicine, Shanghai, China.
authors.forename: Aihong
authors.initials: A
authors.lastname: Song
authors.affiliation: Center for Translational Neurodegeneration and Regenerative Therapy, Shanghai Tenth People's Hospital affiliated to Tongji University School of Medicine, Shanghai, China.
authors.forename: Siqiang
authors.initials: S
authors.lastname: Lai
authors.affiliation: Center for Translational Neurodegeneration and Regenerative Therapy, Shanghai Tenth People's Hospital affiliated to Tongji University School of Medicine, Shanghai, China.
authors.forename: Lisha
authors.initials: L
authors.lastname: Qiu
authors.affiliation: Center for Translational Neurodegeneration and Regenerative Therapy, Shanghai Tenth People's Hospital affiliated to Tongji University School of Medicine, Shanghai, China ; Department of Pharmacology & Experimental Neuroscience, University of Nebraska Medical Center, Omaha, NE 68198, United States ; Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NE 68198, United States.
authors.forename: Yunlong
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Center for Translational Neurodegeneration and Regenerative Therapy, Shanghai Tenth People's Hospital affiliated to Tongji University School of Medicine, Shanghai, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Center for Translational Neurodegeneration and Regenerative Therapy, Shanghai Tenth People's Hospital affiliated to Tongji University School of Medicine, Shanghai, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Zhu
authors.affiliation: Center for Translational Neurodegeneration and Regenerative Therapy, Shanghai Tenth People's Hospital affiliated to Tongji University School of Medicine, Shanghai, China ; Department of Pharmacology & Experimental Neuroscience, University of Nebraska Medical Center, Omaha, NE 68198, United States ; Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NE 68198, United States.
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Xu
authors.affiliation: Center for Translational Neurodegeneration and Regenerative Therapy, Shanghai Tenth People's Hospital affiliated to Tongji University School of Medicine, Shanghai, China ; Department of Pharmacology & Experimental Neuroscience, University of Nebraska Medical Center, Omaha, NE 68198, United States ; Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NE 68198, United States.
authors.forename: Jialin C
authors.initials: JC
authors.lastname: Zheng