title: Effects of Focused Extracorporeal Shock Waves on Bone Marrow Mesenchymal Stem Cells in Patients with Avascular Necrosis of the Femoral Head.
abstract: To observe the effect of extracorporeal shock waves (ESWs) on bone marrow mesenchymal stem cells (MSCs) in patients with avascular necrosis of the femoral head, we collected bone marrow donated by patients and then cultivated and passaged MSCs in vitro using density gradient centrifugation combined with adherence screening methods. The P3 generation MSCs were divided into the ESW group and the control group. The cell counting kit for MSCs detected some proliferation differences. Cytochemistry, alkaline phosphatase staining and Alizarin red staining were used to determine alkaline phosphatase content. Simultaneously, real-time polymerase factor α1, osteocalcin and peroxisome proliferator-activated receptor γ. Together, the results of our study first indicate that moderate ESW intensity, which is instrumental in enhancing MSC proliferation, inducing conversion of MSCs into osteoblasts, and inhibiting differentiation of MSCs into adipocytes from MSCs, is one of the effective mechanisms for treating avascular necrosis of the femoral head.
authors.affiliation: Department of Orthopaedic Surgery, The Affiliated Hospital of Logistics College of Chinese People's Armed Police Force, Hedong District, Tianjin 300162, P. R. China; Department of Orthopaedic Surgery, The General Hospital of Tianjin Medical University, Heping District, Tianjin 300052, P. R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhai
authors.affiliation: Department of Nephropathy, The Affiliated Hospital of Logistics College of Chinese People's Armed Police Force, Hedong District, Tianjin 300162, P. R. China.
authors.forename: Nan
authors.initials: N
authors.lastname: Sun
authors.affiliation: Department of Immunology, Tianjin Key Laboratory of Cellular and Molecular Immunology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Department of Orthopaedic Surgery, The General Hospital of Tianjin Medical University, Heping District, Tianjin 300052, P. R. China.
authors.forename: Shui-Tao
authors.initials: ST
authors.lastname: Liu
authors.affiliation: Department of Orthopaedic Surgery, The General Hospital of Chinese People's Armed Police Force, Haidian District, Beijing 100039, P. R. China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: Department of Orthopaedic Surgery, The General Hospital of Tianjin Medical University, Heping District, Tianjin 300052, P. R. China.
authors.forename: Hai-Chao
authors.initials: HC
authors.lastname: Jin
authors.affiliation: Department of Orthopaedic Surgery, Tianjin Hospital, No 406 Jiefang Nan Road, Hexi District, Tianjin 300211, P. R. China.
authors.forename: Xin-Long
authors.initials: XL
authors.lastname: Ma
authors.affiliation: Department of Orthopaedic Surgery, The General Hospital of Chinese People's Armed Police Force, Haidian District, Beijing 100039, P. R. China. Electronic address: zhangbomianyi@163.com.
authors.forename: Geng-Yan
authors.initials: GY
authors.lastname: Xing
mesh_terms: D000328:Adult; D001854:Bone Marrow Cells / Q000473:pathology / Q000528:radiation effects; D002454:Cell Differentiation / Q000528:radiation effects; D048429:Cell Size / Q000528:radiation effects; D002470:Cell Survival; D005260:Female; D005271:Femur Head Necrosis / Q000473:pathology* / Q000628:therapy*; D019220:High-Energy Shock Waves / Q000627:therapeutic use; D006801:Humans; D008096:Lithotripsy / Q000379:methods*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000473:pathology* / Q000528:radiation effects*; D008875:Middle Aged; D011829:Radiation Dosage; D016896:Treatment Outcome
keywords: Bone marrow mesenchymal stem cells; Extracorporeal shock wave; Femoral head