title: A smart core-sheath nanofiber that captures and releases red blood cells from the blood.
abstract: A smart core-sheath nanofiber for non-adherent cell capture and release is demonstrated. The nanofibers are fabricated by single-spinneret electrospinning of poly(N-isopropylacrylamide) (PNIPAAm), polycaprolactone (PCL) and nattokinase (NK) solution blends. The self-assembly of PNIPAAm and PCL blends during the electrospinning generates the core-sheath PCL/PNIPAAm nanofibers with PNIPAAm as the sheath. The PNIPAAm-based core-sheath nanofibers are switchable between hydrophobicity and hydrophilicity with temperature change and enhance stability in the blood. When the nanofibers come in contact with blood, the NK is released from the nanofibers to resist platelet adhesion on the nanofiber surface, facilitating the direct capture and isolation of red blood cells (RBCs) from the blood above phase-transition temperature of PNIPAAm. Meanwhile, the captured RBCs are readily released from the nanofibers with temperature stimuli in an undamaged manner. The release efficiency of up to 100% is obtained while maintaining cellular integrity and function. This work presents promising nanofibers to effectively capture non-adherent cells and release for subsequent molecular analysis and diagnosis of single cells.
authors.affiliation: State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China. shiqiang@ciac.ac.cn.
authors.forename: Q
authors.initials: Q
authors.lastname: Shi
authors.affiliation: State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China. shiqiang@ciac.ac.cn.
authors.forename: J
authors.initials: J
authors.lastname: Hou
authors.affiliation: Department of Polymer, School of Chemistry and Chemical Engineering, Yantai University, Yantai, 264005, People's Republic of China. xinzhirong2012@126.com.
authors.forename: C
authors.initials: C
authors.lastname: Zhao
authors.affiliation: Department of Polymer, School of Chemistry and Chemical Engineering, Yantai University, Yantai, 264005, People's Republic of China. xinzhirong2012@126.com.
authors.forename: Z
authors.initials: Z
authors.lastname: Xin
authors.affiliation: State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China. shiqiang@ciac.ac.cn.
authors.forename: J
authors.initials: J
authors.lastname: Jin
authors.affiliation: State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China. shiqiang@ciac.ac.cn.
authors.forename: C
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Mechanical Engineering, University of Akron, Akron, Ohio 44325-3903, USA.
authors.forename: S-C
authors.initials: SC
authors.lastname: Wong
authors.affiliation: State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China. shiqiang@ciac.ac.cn.
authors.forename: J
authors.initials: J
authors.lastname: Yin
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D000818:Animals; D002469:Cell Separation / Q000379:methods*; D004912:Erythrocytes / Q000166:cytology*; D057139:Nanofibers / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry*; D011817:Rabbits; D013381:Subtilisins / Q000737:chemistry*
chemical_list: D000180:Acrylic Resins; D011091:Polyesters; C016240:polycaprolactone; C052970:poly-N-isopropylacrylamide; D013381:Subtilisins; C053771:nattokinase