title: H19 derived microRNA-675 regulates cell proliferation and migration through CDK6 in glioma.
abstract: The long non-coding RNA (LncRNA) H19 is one of the most highly abundant and conserved transcripts involved in the mammalian development and tumorigenesis. H19 is expressed in both embryonic cells and tumor cells, but its physical and pathological functions still need to be further studied. Our results showed that microRNA-675, a microRNA in the first exon of H19, expressed in glioma. Over-expression of microRNA-675 in a range of glioma cell lines resulted in their immoderate proliferation and migration. In addition, H19 derived microRNA-675 was down-regulated in the glioma, and CDK6, a pivotal regulator in cell cycle, was a target of microRNA-675. The survival of glioma patients with low CDK6 expression significantly increased as compared to patients with high CDK6 expression. Moreover, the CDK6 expression was inversely correlated with microRNA-675 expression in the glioma. Our results suggest that H19 derived microRNA-675 may regulate giloma cell proliferation and migration through CDK6, and predict a poor prognosis of glioma patients.
authors.affiliation: Department of Neurosurgery, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University Guangzhou 510120, China ; Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University Guangzhou 510120, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Neurosurgery, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University Guangzhou 510120, China.
authors.forename: Bingxi
authors.initials: B
authors.lastname: Lei
authors.affiliation: Department of Neurosurgery, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University Guangzhou 510120, China.
authors.forename: Shuaibin
authors.initials: S
authors.lastname: Huang
authors.affiliation: Department of Neurosurgery, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University Guangzhou 510120, China.
authors.forename: Meiguang
authors.initials: M
authors.lastname: Zheng
authors.affiliation: Department of Neurosurgery, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University Guangzhou 510120, China.
authors.forename: Zhenghao
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Neurosurgery, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University Guangzhou 510120, China.
authors.forename: Zhongjun
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Neurosurgery, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University Guangzhou 510120, China.
authors.forename: Yuefei
authors.initials: Y
authors.lastname: Deng
keywords: H19; glioma; microRNA-675