title: Bacterial Species and Biochemical Characteristic Investigations of Nostoc flagelliforme Concentrates during its Storage.
abstract: Preservation of fresh algae plays an important role in algae seed subculture and aquaculture. The determination and examination of the changes of cell viability, composition, and bacterial species during storage would help to take suitable preservation methods to prolong the preservation time of fresh algae. Nostoc flagelliforme is a kind of edible cyanobacterium with important herbal and dietary values. This article investigated the changes of bacterial species and biochemical characteristics of fresh N. flagelliforme concentrate during natural storage. It was found that the viability of cells decreased along with the storage time. Fourteen bacteria strains in the algae concentrate were identified by PCR-DGGE and were grouped into four phyla, including Cyanobacteria, Firmicutes, Proteobacteria, and Bacteroidetes. Among them, Enterococcus viikkiensis may be a concern in the preservation. Eleven volatile organic compounds were identified from N. flagelliforme cells, in which geosmin could be treated as an indicator of the freshness of N. flagelliforme. The occurrence of indole compound may be an indicator of the degradation of cells.
authors.affiliation: Key Laboratory of Industrial Fermentation Microbiology (Tianjin University of Science & Technology), Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, P.R. China.
authors.forename: Lifang
authors.initials: L
authors.lastname: Yue
authors.affiliation: Key Laboratory of Industrial Fermentation Microbiology (Tianjin University of Science & Technology), Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, P.R. China.
authors.forename: Hexin
authors.initials: H
authors.lastname: Lv
authors.affiliation: Department of Medicinal Chemistry, Ernest Mario School of Pharmacy, Rutgers University, NJ 08854, USA.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhen
authors.affiliation: Research Center for Modern Analysis Techniques, Tianjin University of Science and Technology, Tianjin 300457, P.R. China.
authors.forename: Shengping
authors.initials: S
authors.lastname: Jiang
authors.affiliation: Key Laboratory of Industrial Fermentation Microbiology (Tianjin University of Science & Technology), Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, P.R. China.
authors.forename: Shiru
authors.initials: S
authors.lastname: Jia
authors.affiliation: Key Laboratory of Industrial Fermentation Microbiology (Tianjin University of Science & Technology), Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, P.R. China.
authors.forename: Shigang
authors.initials: S
authors.lastname: Shen
authors.affiliation: Key Laboratory of Industrial Fermentation Microbiology (Tianjin University of Science & Technology), Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, P.R. China.
authors.forename: Lu
authors.initials: L
authors.lastname: Gao
authors.affiliation: Key Laboratory of Industrial Fermentation Microbiology (Tianjin University of Science & Technology), Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, P.R. China.
authors.forename: Yujie
authors.initials: Y
authors.lastname: Dai
mesh_terms: D041963:Bacteroidetes / Q000235:genetics / Q000302:isolation & purification; D003470:Culture Media; D000458:Cyanobacteria / Q000235:genetics / Q000302:isolation & purification; D000068536:Bacillota / Q000235:genetics / Q000302:isolation & purification; D007211:Indoles / Q000032:analysis; D050296:Microbial Viability; D009284:Naphthols / Q000032:analysis; D046937:Nostoc / Q000737:chemistry / Q000145:classification* / Q000254:growth & development / Q000502:physiology*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011309:Preservation, Biological; D020560:Proteobacteria / Q000235:genetics / Q000302:isolation & purification; D055549:Volatile Organic Compounds
chemical_list: D003470:Culture Media; D007211:Indoles; D009284:Naphthols; D055549:Volatile Organic Compounds; C030374:indole; C001278:geosmin
keywords: Nostoc flagelliforme; bacteria; viability; volatile organic compounds