title: Treatment with tanshinone IIA suppresses disruption of the blood-brain barrier and reduces expression of adhesion molecules and chemokines in experimental autoimmune encephalomyelitis.
abstract: Tanshinone IIA (TSIIA), one of the major bioactive components of the traditional Chinese herb Salvia miltiorrhiza, has been reported to have both anti-inflammatory and immunoregulatory effects. The effect of treatment with TSIIA in multiple sclerosis, an autoimmune inflammatory neurodegenerative disease, however, remains poorly understood. In the present study, experimental autoimmune encephalomyelitis (EAE), a classical experimental model of MS, was used to investigate the therapeutic effect of TSIIA. TSIIA attenuated motor dysfunction and improved inflammation and demyelination associated with EAE in a dose-dependent manner. TSIIA also significantly reduced the levels of glial fibrillary acidic protein (GFAP) and ionized calcium-binding adapter molecule-1 (Iba-1), and protected the integrity of the blood-brain barrier (BBB) by increasing the expression of critical endothelial tight junction (TJ) proteins. TSIIA also inhibited the expression of some adhesion molecules and chemokines, which are considered to be critical for adhesion of immune cells and migration across the BBB. TSIIA was thus shown to be effective in the treatment of EAE through preventing the infiltration of immune cells into the CNS, strengthening the integrity of the BBB and decreasing the numbers of adhesion molecules and chemokines.
authors.affiliation: Department of Neurology, Shengjing Hospital of China Medical University, No. 36, Sanhao Street, Shenyang Heping Area, Shenyang, Liaoning Province, PR China. Electronic address: cmu077331snow@163.com.
authors.forename: Xue
authors.initials: X
authors.lastname: Yang
authors.affiliation: Department of Neurology, Shengjing Hospital of China Medical University, No. 36, Sanhao Street, Shenyang Heping Area, Shenyang, Liaoning Province, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Yan
authors.affiliation: Department of Neurology, Shengjing Hospital of China Medical University, No. 36, Sanhao Street, Shenyang Heping Area, Shenyang, Liaoning Province, PR China. Electronic address: fengjuanneurology@yahoo.com.
authors.forename: Juan
authors.initials: J
authors.lastname: Feng
mesh_terms: D045784:Abietanes / Q000494:pharmacology*; D000818:Animals; D001812:Blood-Brain Barrier / Q000187:drug effects*; D002135:Calcium-Binding Proteins / Q000096:biosynthesis / Q000235:genetics; D015815:Cell Adhesion Molecules / Q000037:antagonists & inhibitors* / Q000096:biosynthesis*; D018925:Chemokines / Q000037:antagonists & inhibitors* / Q000096:biosynthesis*; D003711:Demyelinating Diseases / Q000188:drug therapy; D004305:Dose-Response Relationship, Drug; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000188:drug therapy* / Q000378:metabolism* / Q000473:pathology; D005260:Female; D005904:Glial Fibrillary Acidic Protein / Q000096:biosynthesis / Q000235:genetics; D008840:Microfilament Proteins / Q000096:biosynthesis / Q000235:genetics; D009069:Movement Disorders / Q000188:drug therapy / Q000209:etiology; D051381:Rats; D011917:Rats, Inbred Lew; D062725:Tight Junction Proteins / Q000096:biosynthesis / Q000235:genetics
chemical_list: D045784:Abietanes; C100745:Aif1 protein, rat; D002135:Calcium-Binding Proteins; D015815:Cell Adhesion Molecules; D018925:Chemokines; D005904:Glial Fibrillary Acidic Protein; D008840:Microfilament Proteins; D062725:Tight Junction Proteins; C021751:tanshinone
keywords: Adhesion molecules; Blood-brain barrier; Chemokines; Experimental autoimmune encephalomyelitis; Tanshinone IIA; Tanshinone IIA (PubChem CID: 164676).