title: Gene expression profiling combined with functional analysis identify integrin beta1 (ITGB1) as a potential prognosis biomarker in triple negative breast cancer.
abstract: Triple negative breast cancer (TNBC) accounts for approximately 15-20% of all types of breast cancer, and treatment is still limited. This type of breast cancer shows a high risk of recurrence, visceral metastasis, a worse prognosis, and shorter distant metastasis-free survival. Several studies have been reported that genetics factors are associated with breast cancer disease progression and patients' survival. In this study, we combined Taiwanese microarray data from the GEO database and The Cancer Genome Atlas (TCGA) database to study the role of Integrin Beta1 (ITGB1) in TNBC. Two triple negative breast cancer cell lines (MDA-MB-231; MDA-MB-468) were used to validate the functions of ITGB1. We found that a higher ITGB1 gene expression level was associated to lower survival. Silencing of ITGB1 inhibited TNBC cell migration, invasion and store-operated calcium influx. Our study provided a potential candidate biomarker for breast cancer cells migration, invasion and TNBC patients' survival.
authors.affiliation: Department of Clinical Pharmacy, School of Pharmacy, Taipei Medical University, Taipei City 110, Taiwan.
authors.forename: Sukhontip
authors.initials: S
authors.lastname: Klahan
authors.affiliation: Institute of Cellular and Organismic Biology, Academia Sinica, Taipei City 115, Taiwan.
authors.forename: Wan-Chen
authors.initials: WC
authors.lastname: Huang
authors.affiliation: Department of Clinical Pharmacy, School of Pharmacy, Taipei Medical University, Taipei City 110, Taiwan; Master Program for Clinical Pharmacogenomics and Pharmacoproteomics, School of Pharmacy, Taipei Medical University, Taipei City 110, Taiwan.
authors.forename: Che-Mai
authors.initials: CM
authors.lastname: Chang
authors.affiliation: Department of Clinical Pharmacy, School of Pharmacy, Taipei Medical University, Taipei City 110, Taiwan; Master Program for Clinical Pharmacogenomics and Pharmacoproteomics, School of Pharmacy, Taipei Medical University, Taipei City 110, Taiwan.
authors.forename: Henry Sung-Ching
authors.initials: HS
authors.lastname: Wong
authors.affiliation: Department of Surgery, Cathay General Hospital, Taipei City 221, Taiwan; School of Medicine, Fu-Jen Catholic University, New Taipei City 242, Taiwan; School of Medicine, Taipei Medical University, Taipei City 110, Taiwan.
authors.forename: Chi-Cheng
authors.initials: CC
authors.lastname: Huang
authors.affiliation: Department of General and Computational Linguistics, University of Tübingen, Germany.
authors.forename: Mei-Shin
authors.initials: MS
authors.lastname: Wu
authors.affiliation: Department of Clinical Pharmacy, School of Pharmacy, Taipei Medical University, Taipei City 110, Taiwan.
authors.forename: Yu-Chiao
authors.initials: YC
authors.lastname: Lin
authors.affiliation: Department of Clinical Pharmacy, School of Pharmacy, Taipei Medical University, Taipei City 110, Taiwan.
authors.forename: Hsing-Fang
authors.initials: HF
authors.lastname: Lu
authors.affiliation: Cancer Center, Kaohsiung Medical University hospital, Kaohsiung City 807, Taiwan.
authors.forename: Ming-Feng
authors.initials: MF
authors.lastname: Hou
authors.affiliation: Department of Clinical Pharmacy, School of Pharmacy, Taipei Medical University, Taipei City 110, Taiwan; Master Program for Clinical Pharmacogenomics and Pharmacoproteomics, School of Pharmacy, Taipei Medical University, Taipei City 110, Taiwan; Department of Pharmacy, Taipei Medical University-Wan Fang Hospital, Taipei City 116, Taiwan; Cancer Center, Kaohsiung Medical University hospital, Kaohsiung City 807, Taiwan; Comprehensive Cancer Center of Taipei Medical University, Taipei 110, Taiwan. Electronic address: wcc@tmu.edu.tw.
authors.forename: Wei-Chiao
authors.initials: WC
authors.lastname: Chang
mesh_terms: D044466:Asian People / Q000235:genetics; D014408:Biomarkers, Tumor / Q000235:genetics*; D002118:Calcium / Q000378:metabolism; D002453:Cell Cycle; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D020868:Gene Silencing; D006801:Humans; D019012:Integrin beta1 / Q000235:genetics* / Q000378:metabolism; D011379:Prognosis; D012333:RNA, Messenger / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics; D013624:Taiwan; D064726:Triple Negative Breast Neoplasms / Q000235:genetics*
chemical_list: D014408:Biomarkers, Tumor; D019012:Integrin beta1; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D002118:Calcium
keywords: Cell migration; ITGB1; Store-operated calcium channel; Triple negative breast cancer