title: Evaluation of hair growth promoting activity of Phyllanthus niruri.
abstract: OBJECTIVE
  This study was designed to investigate the potential Phyllanthus niruri (P. niruri ) extracts in promotion of hair growth.
  
  
  METHODS
  Here, we studied the hair growth promoting activity of petroleum ether extract of P. niruri following its topical administration. Alopecia was induced in albino rats by subcutaneous administration of testosterone for 21 days. Evaluation of hair loss inhibition was done by concurrent administration of extract and monitoring parameters like follicular density, anagen/telogen (A/T) ratio and histological observation of animal skin sections. Finasteride solution was applied topically as standard. In vitro experiments were also performed to study the effect of extract on the activity of 5α-reductase enzyme.
  
  
  RESULTS
  Groups treated with petroleum ether extract of plant showed hair re-growth as reflected by follicular density, A/T ratio and skin sections. Histopathology and morphologic observations of hair re-growth at shaved sites showed active follicular proliferation. In vitro experiments results showed inhibitory activity of petroleum ether extract on type-2 5α-reductase enzyme and an increase in the amount of testosterone with increasing concentrations.
  
  
  CONCLUSIONS
  It could be concluded that petroleum ether extracts of P. niruri might be useful in the treatment of testosterone-induced alopecia in the experimental animal by inhibiting 5α-reductase enzyme.
authors.affiliation: Department of Pharmaceutical Sciences, Dr. Hari Singh Gour Vishwavidyalaya Sagar- 470003 (M.P.),India.
authors.forename: Satish
authors.initials: S
authors.lastname: Patel
authors.affiliation: Department of Pharmaceutical Sciences, Dr. Hari Singh Gour Vishwavidyalaya Sagar- 470003 (M.P.),India.
authors.forename: Vikas
authors.initials: V
authors.lastname: Sharma
authors.affiliation: Department of Pharmaceutical Sciences, Dr. Hari Singh Gour Vishwavidyalaya Sagar- 470003 (M.P.),India.
authors.forename: Nagendra
authors.initials: N
authors.lastname: S Chauhan
authors.affiliation: Institute for Laboratory Medicine Clinical Chemistry and Pathobiochemistry, Charite Universitäts Medizin, Campus Virchow Klinikum, Augustenburgerplatz 1, Berlin, Germany.
authors.forename: Mayank
authors.initials: M
authors.lastname: Thakur
authors.affiliation: Department of Pharmaceutical Sciences, Dr. Hari Singh Gour Vishwavidyalaya Sagar- 470003 (M.P.),India.
authors.forename: Vinod Kumar
authors.initials: VK
authors.lastname: Dixit
keywords: 5α-reductase; Alopecia; Finasteride; Hair growth; Phyllanthus niruri