title: Glycolipids produced by Rouxiella sp. DSM 100043 and isolation of the biosurfactants via foam-fractionation.
abstract: Microorganisms produce a great variety of secondary metabolites that feature surface active and bioactive properties. Those possessing an amphiphilc molecular structure are also termed biosurfactant and are of great interest due to their often unique properties. Rouxiella sp. DSM 100043 is a gram negative enterobacter isolated from peat-bog soil and described as a new biosurfactant producing species in this study. Rouxiella sp. produces glycolipids, biosurfactants with a carbohydrate moiety in its structure. This study characterizes the composition of glycolipids with different hydrophobicities that have been produced during cultivation in a bioreactor and been extracted and purified from separated foam. Using two dimensional nuclear magnetic resonance spectroscopy, the hydrophilic moieties are elucidated as glucose with various acylation sites and as talose within the most polar glycolipids. The presence of 3' hydroxy lauroleic acid as well as myristic and myristoleic acid has been detected.
authors.affiliation: Section II: Technical Biology, Institute of Process Engineering in Life Sciences, Karlsruhe Institute of Technology, Engler-Bunte Ring 1, 76131, Karlsruhe, Germany. johannes.kuegler@kit.edu.
authors.forename: Johannes H
authors.initials: JH
authors.lastname: Kügler
authors.affiliation: Institute of Organic Chemistry, Karlsruhe Institute of Technology, Fritz-Haber Weg 6, 76131, Karlsruhe, Germany. claudia.muhle-goll@kit.edu.
authors.forename: Claudia
authors.initials: C
authors.lastname: Muhle-Goll
authors.affiliation: Section II: Technical Biology, Institute of Process Engineering in Life Sciences, Karlsruhe Institute of Technology, Engler-Bunte Ring 1, 76131, Karlsruhe, Germany. silla.hansen@kit.edu.|Institute for Biological Interfaces 1, Karlsruhe Institute of Technology, Hermann-von-Helmholtz-Platz 1, 76344, Eggenstein-Leopoldshafen, Germany. silla.hansen@kit.edu.
authors.forename: Silla H
authors.initials: SH
authors.lastname: Hansen
authors.affiliation: Section II: Technical Biology, Institute of Process Engineering in Life Sciences, Karlsruhe Institute of Technology, Engler-Bunte Ring 1, 76131, Karlsruhe, Germany. annika.voelp@kit.edu.|Section Applied Mechanics, Institute for Mechanical Process Engineering and Mechanics, Karlsruhe Institute of Technology, Gotthard-Franz-Strasse 3, 76131, Karlsruhe, Germany. annika.voelp@kit.edu.
authors.forename: Annika R
authors.initials: AR
authors.lastname: Völp
authors.affiliation: Department Microbiology of Natural and Technical Interfaces, Institute of Functional Interfaces, Karlsruhe Institute of Technology, Hermann-von-Helmholtz-Platz 1, 76344, Eggenstein-Leopoldshafen, Germany. frank.kirschhoefer@kit.edu.
authors.forename: Frank
authors.initials: F
authors.lastname: Kirschhöfer
authors.affiliation: Department Microbiology of Natural and Technical Interfaces, Institute of Functional Interfaces, Karlsruhe Institute of Technology, Hermann-von-Helmholtz-Platz 1, 76344, Eggenstein-Leopoldshafen, Germany. boris.kuehl@kit.edu.
authors.forename: Boris
authors.initials: B
authors.lastname: Kühl
authors.affiliation: Department Microbiology of Natural and Technical Interfaces, Institute of Functional Interfaces, Karlsruhe Institute of Technology, Hermann-von-Helmholtz-Platz 1, 76344, Eggenstein-Leopoldshafen, Germany. gerald.brenner-weiss@kit.edu.
authors.forename: Gerald
authors.initials: G
authors.lastname: Brenner-Weiss
authors.affiliation: Institute of Organic Chemistry, Karlsruhe Institute of Technology, Fritz-Haber Weg 6, 76131, Karlsruhe, Germany. burkhard.luy@kit.edu.|Institute for Biological Interfaces 4, Karlsruhe Institute of Technology, Hermann-von-Helmholtz-Platz 1, 76344, Eggenstein-Leopoldshafen, Germany. burkhard.luy@kit.edu.
authors.forename: Burkhard
authors.initials: B
authors.lastname: Luy
authors.affiliation: Section II: Technical Biology, Institute of Process Engineering in Life Sciences, Karlsruhe Institute of Technology, Engler-Bunte Ring 1, 76131, Karlsruhe, Germany. christoph.syldatk@kit.edu.
authors.forename: Christoph
authors.initials: C
authors.lastname: Syldatk
authors.affiliation: Section Bioprocess Engineering, Institute of Food Science and Biotechnology, University of Hohenheim, Garbenstr. 25, 70599, Stuttgart, Germany. rudolf.hausmann@uni-hohenheim.de.
authors.forename: Rudolf
authors.initials: R
authors.lastname: Hausmann
keywords: Biosurfactant; Emulsifier; Glycolipid; Hydroxy linoleic acid; Myristic acid; Myristoleic acid; Rouxiella; Serratia; Surfactant; Talose