title: Effect of human umbilical cord mesenchymal stem cells on endometriotic cell proliferation and apoptosis.
abstract: The objective of this study was to observe the effects of human umbilical cord mesenchymal stem cells (UCMSCs) on the proliferation and apoptosis of endometriotic cells. Endometriotic cells and UCMSCs were primarily cultured in vitro. In the experimental group, a UCMSC and endometriotic cell non-contact co-culture system was established. The control group consisted of 1 x 10(5) endometriotic cells cultured alone. The proliferation and apoptosis of endometriotic cells were respectively detected using the MTT method and flow cytometry. The mRNA expression level of the tensin homologue gene (PTEN) in endometriotic cells was detected by reverse transcription-polymerase chain reaction amplification. Compared with the control group, the proliferation of endometriotic cells in the experimental group was clearly inhibited (P < 0.05) and time-dependent (P < 0.05). In addition, the number of apoptotic cells were significantly increased (P < 0.05), and the amount of cells, which entered S phase from G1 phase, decreased significantly. Furthermore, the mRNA expression level of the PTEN gene in the experimental group was significantly higher than in the control group (P < 0.05). These results suggest that UCMSCs might inhibit the proliferation of human endometriotic cells in vitro and promote their apoptosis by upregulating the expression of PTEN.
authors.affiliation: Department of Gynecology and Obstetrics, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong Province, China.
authors.forename: L N
authors.initials: LN
authors.lastname: Xu
authors.affiliation: Department of Surgery, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong Province, China.
authors.forename: N
authors.initials: N
authors.lastname: Lin
authors.affiliation: Department of Gynecology and Obstetrics, The First People's Hospital of Foshan, Foshan, Guangdong Province, China.
authors.forename: B N
authors.initials: BN
authors.lastname: Xu
authors.affiliation: Department of Gynecology and Obstetrics, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong Province, China.
authors.forename: J B
authors.initials: JB
authors.lastname: Li
authors.affiliation: Department of Gynecology and Obstetrics, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong Province, China.
authors.forename: S Q
authors.initials: SQ
authors.lastname: Chen
mesh_terms: D000328:Adult; D017209:Apoptosis; D049109:Cell Proliferation; D002478:Cells, Cultured; D018920:Coculture Techniques; D004715:Endometriosis / Q000473:pathology*; D004717:Endometrium / Q000166:cytology* / Q000473:pathology; D005260:Female; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology; D008875:Middle Aged; D051059:PTEN Phosphohydrolase / Q000235:genetics / Q000378:metabolism; D014470:Umbilical Cord / Q000166:cytology
chemical_list: D051059:PTEN Phosphohydrolase