title: CD147 overexpression promotes tumorigenicity in Chinese hamster ovary cells.
abstract: CD147 overexpresses in many epithelium-originated tumors and plays an important role in tumor migration and invasion. Most studies aim at the role of CD147 in tumor progression using tumor cell models. However, the influence of abnormal overexpression of CD147 on neoplastic transformation of normal cells is unknown. Here, the role of CD147 in malignant phenotype transformation in CHO cells was investigated. Three CHO cell lines that stably overexpressed CD147 (CHO-CD147), EGFP-CD147 (CHO-EGFP-CD147), and EGFP (CHO-EGFP) were generated by transfection of plasmids containing human CD147, EGFP-human CD147, and EGFP genes into CHO cells. Cell migration and invasion were detected by wound healing and transwell matrix penetration assay. Trypan blue exclusion, MTT, cell cycle analysis, and BrdU cell proliferation assay were used to detect cell viability and cell proliferation. Annexin V-FITC analysis was performed to detect apoptosis. We found that CD147 overexpression promoted the migration and invasion of CHO cells. CD147 accelerated the G1 to S phase transition and enhanced the CHO cell proliferation. Overexpression of CD147 inhibited both early- and late-stages of apoptosis of CHO-CD147 cells, which is caused by serum deprivation. CHO-EGFP-CD147 cells showed an increased anchorage-independent growth compared with CHO-EGFP cells as detected by soft-agar colony formation assay. The tumors formed by CHO-CD147 cells in nude mice were larger and coupled with higher expression of proliferating cell nuclear antigen and Ki-67 than that of CHO cells. In conclusion, human CD147 overexpression induces malignant phenotype in CHO cells.
authors.affiliation: State Key Laboratory of Cancer Biology, Department of Cell Biology, Cell Engineering Research Center, Fourth Military Medical University, Xi'an, 710032, China.
authors.forename: Yu-Le
authors.initials: YL
authors.lastname: Yong
authors.affiliation: Department of Oncology, Urumqi General Hospital of Lanzhou Military Command of PLA, Urumqi, 830000, China.
authors.forename: Cheng-Gong
authors.initials: CG
authors.lastname: Liao
authors.affiliation: State Key Laboratory of Cancer Biology, Department of Cell Biology, Cell Engineering Research Center, Fourth Military Medical University, Xi'an, 710032, China.
authors.forename: Ding
authors.initials: D
authors.lastname: Wei
authors.affiliation: State Key Laboratory of Cancer Biology, Department of Cell Biology, Cell Engineering Research Center, Fourth Military Medical University, Xi'an, 710032, China.
authors.forename: Zhi-Nan
authors.initials: ZN
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Cancer Biology, Department of Cell Biology, Cell Engineering Research Center, Fourth Military Medical University, Xi'an, 710032, China.
authors.forename: Huijie
authors.initials: H
authors.lastname: Bian
mesh_terms: D000818:Animals; D017209:Apoptosis; D051926:Basigin / Q000235:genetics / Q000378:metabolism*; D016466:CHO Cells; D002465:Cell Movement; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic; D006224:Cricetinae; D003412:Cricetulus; D016193:G1 Phase; D006801:Humans; D019394:Ki-67 Antigen / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009369:Neoplasms / Q000378:metabolism / Q000473:pathology; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D018809:Proliferating Cell Nuclear Antigen / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D016196:S Phase
chemical_list: D019394:Ki-67 Antigen; D018809:Proliferating Cell Nuclear Antigen; D051926:Basigin
keywords: EMPPRIN; malignant phenotype; metastasis