title: An essential cell cycle regulation gene causes hybrid inviability in Drosophila.
abstract: Speciation, the process by which new biological species arise, involves the evolution of reproductive barriers, such as hybrid sterility or inviability between populations. However, identifying hybrid incompatibility genes remains a key obstacle in understanding the molecular basis of reproductive isolation. We devised a genomic screen, which identified a cell cycle-regulation gene as the cause of male inviability in hybrids resulting from a cross between Drosophila melanogaster and D. simulans. Ablation of the D. simulans allele of this gene is sufficient to rescue the adult viability of hybrid males. This dominantly acting cell cycle regulator causes mitotic arrest and, thereby, inviability of male hybrid larvae. Our genomic method provides a facile means to accelerate the identification of hybrid incompatibility genes in other model and nonmodel systems.
authors.affiliation: Department of Biology, University of Utah, Salt Lake City, UT 84112, USA. nitin.phadnis@utah.edu hsmalik@fhcrc.org.
authors.forename: Nitin
authors.initials: N
authors.lastname: Phadnis
authors.affiliation: Basic Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA.
authors.forename: EmilyClare P
authors.initials: EP
authors.lastname: Baker
authors.affiliation: Department of Biology, University of Utah, Salt Lake City, UT 84112, USA.
authors.forename: Jacob C
authors.initials: JC
authors.lastname: Cooper
authors.affiliation: Department of Biology, University of Utah, Salt Lake City, UT 84112, USA.
authors.forename: Kimberly A
authors.initials: KA
authors.lastname: Frizzell
authors.affiliation: Basic Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA.
authors.forename: Emily
authors.initials: E
authors.lastname: Hsieh
authors.affiliation: Basic Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA.
authors.forename: Aida Flor A
authors.initials: AF
authors.lastname: de la Cruz
authors.affiliation: Genome Sciences, University of Washington, Seattle, WA 98195, USA. Howard Hughes Medical Institute, University of Washington, Seattle, WA 98195, USA.
authors.forename: Jay
authors.initials: J
authors.lastname: Shendure
authors.affiliation: Genome Sciences, University of Washington, Seattle, WA 98195, USA. Department of Human Genetics, University of Michigan, Ann Arbor, MI 48109, USA.
authors.forename: Jacob O
authors.initials: JO
authors.lastname: Kitzman
authors.affiliation: Basic Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA. Howard Hughes Medical Institute, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA. nitin.phadnis@utah.edu hsmalik@fhcrc.org.
authors.forename: Harmit S
authors.initials: HS
authors.lastname: Malik
mesh_terms: D000483:Alleles; D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000502:physiology*; D002453:Cell Cycle / Q000235:genetics*; D002678:Chimera / Q000235:genetics; D003433:Crosses, Genetic; D004331:Drosophila melanogaster / Q000235:genetics* / Q000254:growth & development; D000067636:Drosophila simulans / Q000235:genetics* / Q000254:growth & development; D018507:Gene Expression Regulation, Developmental; D020043:Genes, Essential / Q000235:genetics / Q000502:physiology; D017344:Genes, Insect; D005804:Genes, Lethal / Q000235:genetics / Q000502:physiology*; D049810:Genetic Speciation; D008297:Male; D008969:Molecular Sequence Data; D060047:Reproductive Isolation
chemical_list: D002352:Carrier Proteins; C494526:GFZF protein, Drosophila