title: Growth and lipid accumulation in three Chlorella strains from different regions in response to diurnal temperature fluctuations.
abstract: It was economically feasible to screen strains adaptive to wide temperature fluctuation for outdoor cultivation without temperature control. In this research, three Chlorella strains from arctic glacier, desert soil and temperate native lake were isolated and identified. The growth, biochemical composition, lipid content and fatty acid composition of each strain cultured under the mode of diurnal temperature fluctuations were compared. All the three Chlorella strains showed desirable abilities of accumulating lipid under diurnal temperature fluctuations and their fatty acid profiles were suitable for biodiesel production, although the growth and biochemical composition were seemed to be region-specific. The highest lipid content was at 51.83±2.49% DW, 42.80±2.97% DW and 36.13±2.27% DW under different temperature fluctuation of 11 °C, 25 °C, 7 °C, respectively. The results indicated that the three Chlorella strains could be promising biodiesel feedstock for outdoor cultivation by the cultural mode of diurnal temperature fluctuations.
authors.affiliation: Jiangsu Provincial Key Laboratory of Marine Biology, College of Resources and Environmental Science, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Weinan
authors.initials: W
authors.lastname: Yang
authors.affiliation: Jiangsu Provincial Key Laboratory of Marine Biology, College of Resources and Environmental Science, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Shanmei
authors.initials: S
authors.lastname: Zou
authors.affiliation: Jiangsu Provincial Key Laboratory of Marine Biology, College of Resources and Environmental Science, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Meilin
authors.initials: M
authors.lastname: He
authors.affiliation: Jiangsu Provincial Key Laboratory of Marine Biology, College of Resources and Environmental Science, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Cong
authors.initials: C
authors.lastname: Fei
authors.affiliation: Key Laboratory for Polar Science of State Oceanic Administration, Polar Research Institute of China, Shanghai 200136, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Luo
authors.affiliation: Jiangsu Provincial Key Laboratory of Marine Biology, College of Resources and Environmental Science, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Shiyan
authors.initials: S
authors.lastname: Zheng
authors.affiliation: Key Laboratory for Polar Science of State Oceanic Administration, Polar Research Institute of China, Shanghai 200136, PR China.
authors.forename: Bo
authors.initials: B
authors.lastname: Chen
authors.affiliation: Jiangsu Provincial Key Laboratory of Marine Biology, College of Resources and Environmental Science, Nanjing Agricultural University, Nanjing 210095, PR China. Electronic address: chwang@njau.edu.cn.
authors.forename: Changhai
authors.initials: C
authors.lastname: Wang
mesh_terms: D020418:Algal Proteins / Q000032:analysis; D001110:Arctic Regions; D056804:Biofuels; D018533:Biomass; D002708:Chlorella / Q000254:growth & development* / Q000302:isolation & purification / Q000378:metabolism*; D002734:Chlorophyll / Q000032:analysis; D002940:Circadian Rhythm; D005227:Fatty Acids / Q000096:biosynthesis; D008055:Lipids / Q000096:biosynthesis*; D010802:Phylogeny; D013696:Temperature
chemical_list: D020418:Algal Proteins; D056804:Biofuels; D005227:Fatty Acids; D008055:Lipids; D002734:Chlorophyll
keywords: Chlorella; Diurnal temperature fluctuation; Fatty acid; Growth; Lipid content