title: Generation and Characterization of Rat Monoclonal Antibodies Against Epidermal Growth Factor Receptor.
abstract: Overexpression of the epidermal growth factor receptor (EGFR) gene and dysregulation of EGFR signaling are observed in various cancer cells, and EGFR is a validated target for cancer therapy. In the present study, we report on the generation of two rat anti-EGFR antibodies (clones 2C2D3 and 4H7F4) by using the rat lymph node method. Flow cytometric analysis and immunofluorescence showed that both antibodies specifically bound to EGFR on the surface of cancer cells. Competitive analysis demonstrated that the epitope of each antibody had no overlap with that of the therapeutic anti-EGFR antibody cetuximab. These results suggest that 2C2D3 and 4H7F4 are potentially useful in EGFR-targeted cancer therapy.
authors.affiliation: 1 Department of Applied Chemistry and Bioengineering, Graduate School of Engineering, Osaka City University , Sumiyoshi-ku, Osaka, Japan .
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Osaki
authors.affiliation: 1 Department of Applied Chemistry and Bioengineering, Graduate School of Engineering, Osaka City University , Sumiyoshi-ku, Osaka, Japan .
authors.forename: Cai-Xia
authors.initials: CX
authors.lastname: Wang
authors.affiliation: 1 Department of Applied Chemistry and Bioengineering, Graduate School of Engineering, Osaka City University , Sumiyoshi-ku, Osaka, Japan .|2 Cell Engineering Corporation, Tennoji-ku, Osaka, Japan .
authors.forename: Taro
authors.initials: T
authors.lastname: Tachibana
authors.affiliation: 1 Department of Applied Chemistry and Bioengineering, Graduate School of Engineering, Osaka City University , Sumiyoshi-ku, Osaka, Japan .
authors.forename: Masayuki
authors.initials: M
authors.lastname: Azuma
authors.affiliation: 1 Department of Applied Chemistry and Bioengineering, Graduate School of Engineering, Osaka City University , Sumiyoshi-ku, Osaka, Japan .
authors.forename: Masaya
authors.initials: M
authors.lastname: Kitamura
authors.affiliation: 1 Department of Applied Chemistry and Bioengineering, Graduate School of Engineering, Osaka City University , Sumiyoshi-ku, Osaka, Japan .
authors.forename: Takeshi
authors.initials: T
authors.lastname: Nakanishi
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000737:chemistry / Q000276:immunology; D000918:Antibody Specificity; D002459:Cell Fusion; D045744:Cell Line, Tumor; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D066246:ErbB Receptors / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D005260:Female; D015870:Gene Expression; D057809:HEK293 Cells; D006801:Humans; D006825:Hybridomas / Q000166:cytology / Q000276:immunology*; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology; D008214:Lymphocytes / Q000166:cytology / Q000276:immunology; D051379:Mice; D009101:Multiple Myeloma / Q000276:immunology / Q000473:pathology; D041681:NIH 3T3 Cells; D010455:Peptides / Q000008:administration & dosage / Q000235:genetics / Q000378:metabolism; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D051381:Rats; D011921:Rats, Inbred WKY
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D010455:Peptides; C512478:EGFR protein, human; D066246:ErbB Receptors