title: Quercetin Alleviates High-Fat Diet-Induced Oxidized Low-Density Lipoprotein Accumulation in the Liver: Implication for Autophagy Regulation.
abstract: A growing body of evidence has indicated that high-fat diet-induced nonalcoholic fatty liver disease is usually accompanied by oxidized low-density lipoprotein (ox-LDL) deposited in the liver. The current study aimed to investigate the effect of quercetin on high-fat diet-induced ox-LDL accumulation in the liver and to explore the potential underlying mechanisms. The results demonstrate that quercetin supplementation for 24 weeks significantly alleviated high-fat diet-induced liver damage and reduced hepatic cholesterol and ox-LDL level. Quercetin notably inhibited both mRNA and protein expression of CD36 (reduced by 53% and 71%, resp.) and MSR1 (reduced by 25% and 45%, resp.), which were upregulated by high-fat diet. The expression of LC3II was upregulated by 2.4 times whereas that of p62 and mTOR was downregulated by 57% and 63% by quercetin treatment. Therefore, the significantly improved autophagy lysosomal degradation capacity for ox-LDL may be implicated in the hepatoprotective effect of quercetin; scavenger receptors mediated ox-LDL uptake might also be involved.
authors.affiliation: College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China ; Hubei Collaborative Innovation Center for Processing of Agricultural Products, Wuhan Polytechnic University, Wuhan 430023, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Liu
authors.affiliation: National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Gao
authors.affiliation: Department of Nutrition and Food Hygiene, Hubei Key Laboratory of Food Nutrition and Safety, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Yao
authors.affiliation: College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China ; Hubei Collaborative Innovation Center for Processing of Agricultural Products, Wuhan Polytechnic University, Wuhan 430023, China.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Gong
mesh_terms: D000818:Animals; D001343:Autophagy / Q000187:drug effects*; D018955:CD36 Antigens / Q000096:biosynthesis; D002784:Cholesterol / Q000378:metabolism; D059305:Diet, High-Fat / Q000009:adverse effects; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008099:Liver / Q000378:metabolism / Q000473:pathology; D051379:Mice; D008869:Microtubule-Associated Proteins / Q000096:biosynthesis; D065626:Non-alcoholic Fatty Liver Disease / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D011794:Quercetin / Q000008:administration & dosage*; D012333:RNA, Messenger / Q000096:biosynthesis; D051117:Scavenger Receptors, Class A / Q000096:biosynthesis; D058570:TOR Serine-Threonine Kinases / Q000096:biosynthesis
chemical_list: D018955:CD36 Antigens; D008077:Lipoproteins, LDL; D008869:Microtubule-Associated Proteins; C495140:Msr1 protein, mouse; D012333:RNA, Messenger; D051117:Scavenger Receptors, Class A; C099252:oxidized low density lipoprotein; D002784:Cholesterol; D011794:Quercetin; D058570:TOR Serine-Threonine Kinases