title: Single nucleotide polymorphisms of the SLC22A2 gene within the Xhosa population of South Africa.
abstract: Human organic cation transporter 2 (hOCT2) is thought to play a critical role in the uptake, pharmacological effects and/or adverse effects of many cationic clinical therapeutics and xenobiotics. Moreover, genetic variations in hOCT2 gene, SLC22A2, are increasingly being recognized as a possible mechanism that can explain individual variation in drug response. To screen for variations in this gene, SLC22A2 was directly sequenced in 96 healthy Xhosa individuals. A total of 27 variations, including three novel ones, were identified in SLC22A2: eight in exons, 15 in introns, and four in the 5'-untranslated region. The minor allele frequencies (MAF) of genetic variants observed in the Xhosa population were compared both to other African and other world populations. Seventeen of the variants observed in the SLC22A2 gene of the Xhosa population were specific to/or occurred at a higher frequency in African populations or populations with a recent connection to the African continent.
authors.affiliation: Department of Biotechnology, University of the Western Cape, Robert Sobukwe Road, Bellville 7535, Cape Town, South Africa.
authors.forename: Clifford
authors.initials: C
authors.lastname: Jacobs
authors.affiliation: Department of Biotechnology, University of the Western Cape, Robert Sobukwe Road, Bellville 7535, Cape Town, South Africa.
authors.forename: Brendon
authors.initials: B
authors.lastname: Pearce
authors.affiliation: Department of Biotechnology, University of the Western Cape, Robert Sobukwe Road, Bellville 7535, Cape Town, South Africa.
authors.forename: Mornè
authors.initials: M
authors.lastname: Du Plessis
authors.affiliation: Department of Biotechnology, University of the Western Cape, Robert Sobukwe Road, Bellville 7535, Cape Town, South Africa.
authors.forename: Nisreen
authors.initials: N
authors.lastname: Hoosain
authors.affiliation: Department of Biotechnology, University of the Western Cape, Robert Sobukwe Road, Bellville 7535, Cape Town, South Africa. Electronic address: mbenjeddou@uwc.ac.za.
authors.forename: Mongi
authors.initials: M
authors.lastname: Benjeddou
mesh_terms: D020121:5' Untranslated Regions; D044383:Black People / Q000235:genetics*; D005091:Exons; D005787:Gene Frequency; D006801:Humans; D007438:Introns; D027701:Organic Cation Transport Proteins / Q000235:genetics*; D000075102:Organic Cation Transporter 2; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA; D013019:South Africa
chemical_list: D020121:5' Untranslated Regions; D027701:Organic Cation Transport Proteins; D000075102:Organic Cation Transporter 2; C491013:SLC22A2 protein, human
keywords: African populations; Genetic variability; Haplotype; Polymorphism; SLC22A2