title: Blood levels of serotonin are specifically correlated with plasma lysophosphatidylserine among the glycero-lysophospholipids.
abstract: BACKGROUND
  Glycero-lysophospholipids (glycero-LPLs), which are known to exert potent biological activities, have been demonstrated to be secreted from activated platelets in vitro; however, their association with platelet activation in vivo has not been yet elucidated. In this study, we investigated the correlations between the blood levels of each glycero-LPL and serotonin, a biomarker of platelet activation, in human subjects to elucidate the involvement of platelet activation in glycero-LPLs in vivo.
  
  
  RESULTS
  We measured the plasma serotonin levels in 141 consecutive patients undergoing coronary angiography (acute coronary syndrome, n = 38; stable angina pectoris, n = 71; angiographically normal coronary arteries, n = 32) and investigated the correlations between the plasma levels of serotonin and glycero-LPLs. The results revealed the existence of a specific and significant association between the plasma serotonin and plasma lysophosphatidylserine (LysoPS) levels. On the contrary, regular aspirin intake failed to affect the plasma LysoPS levels despite the fact that the plasma lysophosphatidic acid, lysophosphatidylethanolamine, lysophosphatidylglycerol, and lysophosphatidylinositol levels were lower in those who had taken aspirin regularly.
  
  
  CONCLUSIONS
  We found a specific positive correlation between the blood levels of serotonin and LysoPS, a new lipid mediator. Thus, LysoPS might be specifically involved in strong platelet activation, which is associated with the release of serotonin.
  
  
  CONCLUSIONS
  Our present results suggest the possible involvement of LysoPS in the pathogenesis of atherosclerotic diseases.
authors.affiliation: The University of Tokyo, Department of Clinical Laboratory Medicine, Graduate School of Medicine, Tokyo, Japan ; CREST, Japan Science and Technology Corporation (JST), Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Kurano
authors.affiliation: Juntendo University School of Medicine, Department of Cardiovascular Medicine, Japan.
authors.forename: Tomotaka
authors.initials: T
authors.lastname: Dohi
authors.affiliation: The University of Tokyo Hospital, Department of Clinical Laboratory, Tokyo, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Nojiri
authors.affiliation: The University of Tokyo Hospital, Department of Clinical Laboratory, Tokyo, Japan.
authors.forename: Tamaki
authors.initials: T
authors.lastname: Kobayashi
authors.affiliation: Bioscience Division, TOSOH Corporation, Kanagawa, Japan ; Saitama Prefectural University, Laboratory Science, Department of Health Science, Saitama, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Hirowatari
authors.affiliation: Tohoku University, Laboratory of Molecular and Cellular Biochemistry, Graduate School of Pharmaceutical Sciences, Miyagi, Japan ; PRESTO, Japan Science and Technology Corporation (JST), Japan.
authors.forename: Asuka
authors.initials: A
authors.lastname: Inoue
authors.affiliation: CREST, Japan Science and Technology Corporation (JST), Japan ; Tohoku University, Laboratory of Molecular and Cellular Biochemistry, Graduate School of Pharmaceutical Sciences, Miyagi, Japan.
authors.forename: Kuniyuki
authors.initials: K
authors.lastname: Kano
authors.affiliation: Tohoku University, Laboratory of Molecular and Cellular Biochemistry, Graduate School of Pharmaceutical Sciences, Miyagi, Japan.
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Matsumoto
authors.affiliation: Bioscience Division, TOSOH Corporation, Kanagawa, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Igarashi
authors.affiliation: The University of Tokyo, Department of Clinical Laboratory Medicine, Graduate School of Medicine, Tokyo, Japan ; CREST, Japan Science and Technology Corporation (JST), Japan.
authors.forename: Masako
authors.initials: M
authors.lastname: Nishikawa
authors.affiliation: Juntendo University School of Medicine, Department of Cardiovascular Medicine, Japan.
authors.forename: Katsumi
authors.initials: K
authors.lastname: Miyauchi
authors.affiliation: Juntendo University School of Medicine, Department of Cardiovascular Medicine, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Daida
authors.affiliation: The University of Tokyo, Department of Clinical Laboratory Medicine, Graduate School of Medicine, Tokyo, Japan ; CREST, Japan Science and Technology Corporation (JST), Japan ; The University of Tokyo Hospital, Department of Clinical Laboratory, Tokyo, Japan.
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Ikeda
authors.affiliation: CREST, Japan Science and Technology Corporation (JST), Japan ; Tohoku University, Laboratory of Molecular and Cellular Biochemistry, Graduate School of Pharmaceutical Sciences, Miyagi, Japan.
authors.forename: Junken
authors.initials: J
authors.lastname: Aoki
authors.affiliation: The University of Tokyo, Department of Clinical Laboratory Medicine, Graduate School of Medicine, Tokyo, Japan ; CREST, Japan Science and Technology Corporation (JST), Japan ; The University of Tokyo Hospital, Department of Clinical Laboratory, Tokyo, Japan.
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Yatomi
keywords: ACS, acute coronary syndrome; Acute coronary syndrome; Aspirin; Glycero-LPL, glycero-lysophospholipid; Glycero-lysophospholipids; LC-MS/MS, liquid chromatography-tandem mass spectrometry; LPL, lysophospholipid; LysoPA, lysophosphatidic acids; LysoPC, lysophosphatidylcholine; LysoPE, lysophosphatidylethanolamine; LysoPG, lysophosphatidylglycerol; LysoPI, lysophosphatidylinositol; LysoPS, lysophosphatidylserine; Lysophosphatidylserine; NCA, angiographically normal coronary arteries; PS, phosphatidylserine; PS-PLA1, phosphatidylserine-specific phospholipase A1;; SAP, stable angina pectoris; Serotonin