title: MicroRNA-205 functions as a tumor suppressor in colorectal cancer by targeting cAMP responsive element binding protein 1 (CREB1).
abstract: BACKGROUND
  MicroRNAs (miRNA) have been documented playing critical roles in cancer progression. Aberrant expression of miR-205 has been reported in several types of cancer. However, the role and mechanism of miR-205 in colorectal cancer (CRC) remains unclear.
  
  
  METHODS
  In this study, the expression levels of miR-205 in CRC tissues and cell lines were detected by quantitative real-time PCR (qRT-PCR). MTT assay and transwell assays were applied to assess the effect of miR-205 on CRC cell proliferation, migration and invasion abilities in vitro. Furthermore, Dual-luciferase reporter assay was conducted to confirm the direct binding of miR-205 and target genes.
  
  
  RESULTS
  In the present study, we found that the expression level of miR-205 in CRC tissues and cell lines was significantly down-regulated. Functional assays showed that overexpression of miR-205 suppressed CRC cell proliferation, migration and invasion in vitro. In addition, cAMP responsive element binding protein 1 (CREB1) was identified as targets of miR-205. Silencing CREB1 had similar effects of miR-205 restoration on proliferation, migration and invasion in CRC cells.
  
  
  CONCLUSIONS
  Our results demonstrated that miR-205 may act as a tumor suppressor by targeting the CREB1 gene and suppressing CRC cell proliferation, migration and invasion. Thus, miR-205 may represent a potential therapeutic target for CRC intervention.
authors.affiliation: Department of Surgery, Affiliated Hospital of Nantong University Nantong 226001, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Li
authors.affiliation: Department of Surgery, Affiliated Hospital of Nantong University Nantong 226001, China.
authors.forename: Wan-Jiang
authors.initials: WJ
authors.lastname: Xue
authors.affiliation: Department of Surgery, Affiliated Hospital of Nantong University Nantong 226001, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Feng
authors.affiliation: Department of Surgery, Affiliated Hospital of Nantong University Nantong 226001, China.
authors.forename: Qin-Sheng
authors.initials: QS
authors.lastname: Mao
keywords: Colorectal cancer; cAMP responsive element binding protein 1; miR-205