title: [Validation of ELISA Kits for Pesticide Residue Analysis in Vegetables and Fruits].
abstract: Five kinds of commercially available ELISA kits (acetamiprid, azoxystrobin, chlorothalonil, fenitrothion and imidacloprid) were validated for determination of pesticide residues in vegetables and fruits. The reaction characteristics were also examined to evaluate their influence on the determinations. The trueness value was 91-162%, the repeatability was 2.1-16.2%, and the reproducibility was 4.0-20.3%. The desired values were achieved for 18 among 30 combinations (60%) of the ELISA kits and the agricultural products examined. A standard curve was necessary for each of the ELISA examinations. The matrix of the agricultural products and pipetting skill of the lab technician both influenced the measurment results.
authors.affiliation: Advanced Science Technology & Management Research Institute of KYOTO.
authors.forename: Tomomi
authors.initials: T
authors.lastname: Yamasaki
authors.forename: Tomomi
authors.initials: T
authors.lastname: Inoue
authors.forename: Yuki
authors.initials: Y
authors.lastname: Hirakawa
authors.forename: Shiro
authors.initials: S
authors.lastname: Miyake
authors.forename: Eiji
authors.initials: E
authors.lastname: Ueno
authors.forename: Isao
authors.initials: I
authors.lastname: Saito
mesh_terms: D018556:Crops, Agricultural / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005638:Fruit / Q000737:chemistry*; D010573:Pesticide Residues / Q000032:analysis*; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results; D014675:Vegetables / Q000737:chemistry*
chemical_list: D010573:Pesticide Residues; D011933:Reagent Kits, Diagnostic