title: Nanoscale Metal-Organic Framework-Hemoglobin Conjugates.
abstract: A metal-organic framework (MOF)-protein conjugate, NH2 -MIL-125(Ti)-hemoglobin [MIL-125(Ti)-Hb], was synthesized by a covalent postmodification strategy. The crystalline structure was maintained after chemical and protein modification. The content of grafted Hb was tuned by the stoichiometric ratio and reached 50 wt % if the mass ratio of MIL-125(Ti)/Hb was 1:1.25 in the feed. The oxygen-transporting capacity of grafted Hb was kept, and the P50 (the half O2 pressure saturated with O2 ) and Hill coefficients of the MIL-125(Ti)-Hb conjugate were found to be 22.9 mm Hg and 2.35, respectively, which are close to the respective values of free Hb. All the results indicate that the MIL-125(Ti)-Hb conjugate could be potentially used as an oxygen carrier.
authors.affiliation: State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, P. R. China.|The University of Chinese Academy of Sciences, Beijing, 100049, P. R. China.
authors.forename: Weiqi
authors.initials: W
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, P. R. China. leiwang@ciac.ac.cn.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, P. R. China.
authors.forename: Yubin
authors.initials: Y
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, P. R. China. xiez@ciac.ac.cn.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Xie
authors.affiliation: State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, P. R. China.
authors.forename: Xiabin
authors.initials: X
authors.lastname: Jing
mesh_terms: D000818:Animals; D001802:Blood Substitutes / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D002417:Cattle; D002460:Cell Line; D006454:Hemoglobins / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D006801:Humans; D008958:Models, Molecular; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D010100:Oxygen / Q000378:metabolism; D014025:Titanium / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D001802:Blood Substitutes; D006454:Hemoglobins; D009942:Organometallic Compounds; D014025:Titanium; D010100:Oxygen
keywords: conjugate materials; hemoglobin; metal-organic frameworks; nanoparticles; oxygen carriers