title: Pharmacokinetics of Chiral Dendrimer-Triamine-Coordinated Gd-MRI Contrast Agents Evaluated by in Vivo MRI and Estimated by in Vitro QCM.
abstract: Recently, we developed novel chiral dendrimer-triamine-coordinated Gd-MRI contrast agents (Gd-MRI CAs), which showed longitudinal relaxivity (r₁) values about four times higher than that of clinically used Gd-DTPA (Magnevist(®), Bayer). In our continuing study of pharmacokinetic differences derived from both the chirality and generation of Gd-MRI CAs, we found that the ability of chiral dendrimer Gd-MRI CAs to circulate within the body can be directly evaluated by in vitro MRI (7 T). In this study, the association constants (K(a)) of chiral dendrimer Gd-MRI CAs to bovine serum albumin (BSA), measured and calculated with a quartz crystal microbalance (QCM) in vitro, were found to be an extremely easy means for evaluating the body-circulation ability of chiral dendrimer Gd-MRI CAs. The K(a) values of S-isomeric dendrimer Gd-MRI CAs were generally greater than those of R-isomeric dendrimer Gd-MRI CAs, which is consistent with the results of our previous MRI study in vivo.
authors.affiliation: Department of Energy and Hydrocarbon Chemistry, Graduate School of Engineering, Kyoto University, Nishikyo-ku, Kyoto 615-8510, Japan. miyake.yuka.46c@st.kyoto-u.ac.jp.
authors.forename: Yuka
authors.initials: Y
authors.lastname: Miyake
authors.affiliation: Department of Energy and Hydrocarbon Chemistry, Graduate School of Engineering, Kyoto University, Nishikyo-ku, Kyoto 615-8510, Japan. m0syunc0@gmail.com.
authors.forename: Syungo
authors.initials: S
authors.lastname: Ishikawa
authors.affiliation: Department of Energy and Hydrocarbon Chemistry, Graduate School of Engineering, Kyoto University, Nishikyo-ku, Kyoto 615-8510, Japan. ykimura@scl.kyoto-u.ac.jp.|Research and Educational Unit of Leaders for Integrated Medical System, Center for the Promotion of Interdisciplinary Education and Research, Kyoto University, Nishikyo-ku, Kyoto 615-8510, Japan. ykimura@scl.kyoto-u.ac.jp.
authors.forename: Yu
authors.initials: Y
authors.lastname: Kimura
authors.affiliation: Department of Energy and Hydrocarbon Chemistry, Graduate School of Engineering, Kyoto University, Nishikyo-ku, Kyoto 615-8510, Japan. son.aoi.3n@kyoto-u.ac.jp.
authors.forename: Aoi
authors.initials: A
authors.lastname: Son
authors.affiliation: Graduate School of Informatics, Kyoto University, Yoshida-honmachi, Sakyo-ku, Kyoto 606-8501, Japan. imai@sys.i.kyoto-u.ac.jp.
authors.forename: Hirohiko
authors.initials: H
authors.lastname: Imai
authors.affiliation: Graduate School of Informatics, Kyoto University, Yoshida-honmachi, Sakyo-ku, Kyoto 606-8501, Japan. tetsu@i.kyoto-u.ac.jp.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Matsuda
authors.affiliation: Department of Energy and Hydrocarbon Chemistry, Graduate School of Engineering, Kyoto University, Nishikyo-ku, Kyoto 615-8510, Japan. yamada.hisatsugu@tokushima-u.ac.jp.|Advanced Biomedical Engineering Research Unit, Center for the Promotion of Interdisciplinary Education and Research, Kyoto University, Nishikyo-ku, Kyoto 615-8510, Japan. yamada.hisatsugu@tokushima-u.ac.jp.
authors.forename: Hisatsugu
authors.initials: H
authors.lastname: Yamada
authors.affiliation: Department of Energy and Hydrocarbon Chemistry, Graduate School of Engineering, Kyoto University, Nishikyo-ku, Kyoto 615-8510, Japan. toshi-r@iris.eonet.ne.jp.|Division of Multidisciplinary Chemistry, Institute for Chemical Research, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan. toshi-r@iris.eonet.ne.jp.
authors.forename: Akio
authors.initials: A
authors.lastname: Toshimitsu
authors.affiliation: Department of Energy and Hydrocarbon Chemistry, Graduate School of Engineering, Kyoto University, Nishikyo-ku, Kyoto 615-8510, Japan. teruyuki@scl.kyoto-u.ac.jp.|Advanced Biomedical Engineering Research Unit, Center for the Promotion of Interdisciplinary Education and Research, Kyoto University, Nishikyo-ku, Kyoto 615-8510, Japan. teruyuki@scl.kyoto-u.ac.jp.
authors.forename: Teruyuki
authors.initials: T
authors.lastname: Kondo
mesh_terms: D000818:Animals; D002417:Cattle; D002460:Cell Line; D003287:Contrast Media / Q000737:chemistry* / Q000493:pharmacokinetics*; D050091:Dendrimers / Q000737:chemistry*; D019786:Gadolinium DTPA / Q000737:chemistry*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D051379:Mice; D011073:Polyamines / Q000737:chemistry; D058525:Quartz Crystal Microbalance Techniques / Q000379:methods; D012710:Serum Albumin, Bovine / Q000737:chemistry; D014018:Tissue Distribution
chemical_list: D003287:Contrast Media; D050091:Dendrimers; D011073:Polyamines; D012710:Serum Albumin, Bovine; D019786:Gadolinium DTPA
keywords: association constant (Ka); chiral dendrimer; contrast agent; gadolinium; magnetic resonance imaging (MRI); quartz crystal microbalance (QCM)