title: Genetic diversity and population structure of endangered Aquilaria malaccensis revealed potential for future conservation.
abstract: The endangered Aquilaria malaccensis,is an important plant with high economic values. Characterization of genetic diversity and population structure is receiving tremendous attention for effective conservation of genetic resources. Considering important repositories of biological diversity, the genetic relationships of 127 A. malaccensis accessions from 10 home gardens of three states of northeast India were assessed using amplified fragment length polymorphism (AFLP). Of the 1153 fragments amplified with four AFLP primer combinations, 916 (79.4%) were found to be polymorphic. Polymorphic information content (PIC) and marker index (MI) of each primer combination correlate significantly with the number of genotypes resolved. Overall, a high genetic diversity (avg. 71.85%) was recorded. Further, high gene flow (Nm: 3.37), low genetic differentiation (FST: 0.069) and high within population genetic variation (93%) suggests that most of the genetic diversity is restricted within population. Neighbour joining (NJ), principal coordinate analysis (PCoA) and Bayesian-based STRUCTURE grouped all the accessions in two clusters with significant intermixing between populations, therefore, revealed that two genetically distinct gene pools are operating in the A. malaccensis populations cultivated in home gardens. Based on the various diversity inferences, five diverse populations (JOH, FN, HLF, DHM and ITN) were identified, which can be potentially exploited to develop conservation strategies for A. malaccensis.
authors.affiliation: Division of Biotechnology, CSIR-Institute of Himalayan Bioresource Technology, P.O. Box 6, Palampur 176 061, India. rksharma.ihbt@gmail.com.
authors.forename: Pradeep
authors.initials: P
authors.lastname: Singh
authors.forename: Akshay
authors.initials: A
authors.lastname: Nag
authors.forename: Rajni
authors.initials: R
authors.lastname: Parmar
authors.forename: Sneha
authors.initials: S
authors.lastname: Ghosh
authors.forename: Brijmohan Singh
authors.initials: BS
authors.lastname: Bhau
authors.forename: Ram Kumar
authors.initials: RK
authors.lastname: Sharma
mesh_terms: D017343:Genes, Plant / Q000235:genetics*; D014644:Genetic Variation / Q000235:genetics*; D005828:Genetics, Population / Q000379:methods; D005838:Genotype; D007194:India; D012150:Polymorphism, Restriction Fragment Length / Q000235:genetics*; D029645:Thymelaeaceae / Q000235:genetics*