title: Metabonomic analysis of quercetin against the toxicity of chronic exposure to a mixture of four organophosphate pesticides in rat plasma.
abstract: 1. A metabonomics approach was performed to investigate the effect of quercetin on the toxicity of chronic exposure to a mixture of four organophosphate pesticides (OPs) at their corresponding no-observed-adverse-effect level (NOAEL). The rats were divided into six groups (n = 10/group): control, two different doses of quercetin, OPs mixture and different doses of quercetin plus OPs mixture-treated groups. 2. Nine metabolites, including two quercetin metabolites and seven endogenous metabolites were identified in plasma. The intensities of metabolites significantly changed in the OP mixture-treated group compared with the control group (p < 0.01), such as lysoPE (16:0/0:0), lysoPC (17:0/0:0), lysoPC (15:0/0:0) and 4-pyridoxic acid, significantly increased; by contrast, the intensities of arachidonic acid and citric acid significantly decreased. Anomalous intensity changes in aforementioned metabolites were alleviated in the OP mixture plus 50 mg/kgċbw/d quercetin-treated group compared with the OP mixture-treated group (p < 0.05). 3. The results indicated that quercetin elicited partial protective effects against the toxicity induced by a mixture of OPs, which include regulation of lipid metabolism, improvement of tricarboxylic acid (TCA) cycle disorders, enhancement of antioxidant defence system to protect the liver.
authors.affiliation: a Department of Nutrition and Food Hygiene , Public Health College, Harbin Medical University , Harbin , Heilongjiang , China.
authors.forename: Can
authors.initials: C
authors.lastname: Cao
authors.affiliation: a Department of Nutrition and Food Hygiene , Public Health College, Harbin Medical University , Harbin , Heilongjiang , China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zeng
authors.affiliation: a Department of Nutrition and Food Hygiene , Public Health College, Harbin Medical University , Harbin , Heilongjiang , China.
authors.forename: Haidan
authors.initials: H
authors.lastname: Shi
authors.affiliation: a Department of Nutrition and Food Hygiene , Public Health College, Harbin Medical University , Harbin , Heilongjiang , China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Yang
authors.affiliation: a Department of Nutrition and Food Hygiene , Public Health College, Harbin Medical University , Harbin , Heilongjiang , China.
authors.forename: Wei
authors.initials: W
authors.lastname: Bao
authors.affiliation: a Department of Nutrition and Food Hygiene , Public Health College, Harbin Medical University , Harbin , Heilongjiang , China.
authors.forename: Lei
authors.initials: L
authors.lastname: Qi
authors.affiliation: a Department of Nutrition and Food Hygiene , Public Health College, Harbin Medical University , Harbin , Heilongjiang , China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.affiliation: a Department of Nutrition and Food Hygiene , Public Health College, Harbin Medical University , Harbin , Heilongjiang , China.
authors.forename: Xiujun
authors.initials: X
authors.lastname: Zhao
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D008297:Male; D055442:Metabolome / Q000502:physiology*; D055432:Metabolomics; D019055:No-Observed-Adverse-Effect Level; D009943:Organophosphorus Compounds / Q000633:toxicity*; D010575:Pesticides / Q000633:toxicity*; D011794:Quercetin / Q000494:pharmacology*; D051381:Rats; D036401:Toxicity Tests, Chronic
chemical_list: D000975:Antioxidants; D009943:Organophosphorus Compounds; D010575:Pesticides; D011794:Quercetin
keywords: Metabonomics; UPLC-MS; no observed adverse effect level; organophosphate pesticide mixture; quercetin; rat plasma