title: Inhibition of Vav3 gene can promote apoptosis of human gastric cancer cell line MGC803 by regulating ERK pathway.
abstract: Previous studies proved that Vav3 gene was overexpressed in cancers. However, the molecular mechanism of Vav3 in apoptosis still keeps unclear; therefore, the relationship between Vav3 gene and apoptosis of gastric cancer (GC) was explored in the present study. Vav3-siRNA was transfected into MGC803 cells, and then cell activity and apoptosis rate were tested with MTT and FCM; apoptosis-related genes and proteins in MAPK signaling pathway were also tested. Results showed that Vav3 was overexpressed in GC than in adjacent normal tissues (all P < 0.05), and expression of Vav3 was related to degree of histological differentiation, cancer invasion depth, and lymphatic metastasis (Χ (2)  = 7.185, P = 0.007; Χ (2)  = 18.654, P < 0.001; Χ (2)  = 5.058, P = 0.025). Vav3 silencing inhibited activity of MGC803 cells, and apoptosis rate of cells was affected. Vav3-siRNA transfection led to changes of apoptosis-related genes such as Survivin, xIAP, Bcl-2, caspase-3, and Bax (all P < 0.01). After transfection, ratio of phosphorylation of ERK significantly reduced. We concluded that Vav3 inhibition can suppress cell activity and promote apoptosis by regulating the apoptosis-related genes through the ERK pathway.
authors.affiliation: Department of General Surgery, the Fourth Affiliated Hospital, Hebei Medical University, No.12, Jian-Kang Road, Shijiazhuang, 050011, China.
authors.forename: Bi-Bo
authors.initials: BB
authors.lastname: Tan
authors.affiliation: Department of Medical laboratory, HeBei General Hospital, 348 He-Ping West St, Shijiazhuang, 050051, China.
authors.forename: Ming-Ming
authors.initials: MM
authors.lastname: Zhang
authors.affiliation: Department of General Surgery, the Fourth Affiliated Hospital, Hebei Medical University, No.12, Jian-Kang Road, Shijiazhuang, 050011, China. li_yong_hbth@126.com.
authors.forename: Yong
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of General Surgery, the Fourth Affiliated Hospital, Hebei Medical University, No.12, Jian-Kang Road, Shijiazhuang, 050011, China.
authors.forename: Qun
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: Department of General Surgery, the Fourth Affiliated Hospital, Hebei Medical University, No.12, Jian-Kang Road, Shijiazhuang, 050011, China.
authors.forename: Li-Qiao
authors.initials: LQ
authors.lastname: Fan
authors.affiliation: Department of General Surgery, the Fourth Affiliated Hospital, Hebei Medical University, No.12, Jian-Kang Road, Shijiazhuang, 050011, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of General Surgery, the Fourth Affiliated Hospital, Hebei Medical University, No.12, Jian-Kang Road, Shijiazhuang, 050011, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Wang
mesh_terms: D000368:Aged; D017209:Apoptosis / Q000235:genetics*; D015153:Blotting, Western; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D020935:MAP Kinase Signaling System / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D051567:Proto-Oncogene Proteins c-vav / Q000235:genetics* / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000235:genetics / Q000473:pathology*; D014162:Transfection
chemical_list: D051567:Proto-Oncogene Proteins c-vav; D034741:RNA, Small Interfering; C496621:VAV3 protein, human
keywords: Apoptosis; Gastric cancer; Signal transduction pathway; Small interfering RNA; Vav3 gene